LOCUS       DAOMXG010000001        58023 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_402_length_58023_cov_11.713687, whole genome shotgun sequence.
ACCESSION   DAOMXG010000001 DAOMXG010000000
VERSION     DAOMXG010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 58023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 58023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58023
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_402_length_58023_cov_11.713687"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            144..1118
                     /locus_tag="PLQ11_00005"
     CDS             144..1118
                     /locus_tag="PLQ11_00005"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRK23312.1"
                     /translation="MIKLMRLALAVSISGLAPLASAQSPWPDKPVTVIVPYAAGGNTD
                     VVGRIFTERLSQRLGKPFIVENKSGAGGTIGIAAMSRAPADGYTLGVVTAGNLFILPH
                     IYKDKLGYDTFKDLKPLAMVATQPNFFVVHPSIPATTPKEFVDHLKANPDKYSYGSSG
                     IGTSQHLCMELLAQKTGIKVTHVPYRASNQIMQDLIGGTIHMSCDQISTALAQVRNGR
                     AKGIAVSSPEAYGLAPEYPAMAATVPGIDVTWAAIFIAPANLPKPIADRLTSELGAIA
                     KDPDVIEKLKGLTVTPVSVVGDALEKRIKADFETWKPIVESAKIPPPQ"
     gene            1162..2355
                     /locus_tag="PLQ11_00010"
     CDS             1162..2355
                     /locus_tag="PLQ11_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015952837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HRK23313.1"
                     /translation="MKRNGAFMRRMCLALPLILAALPARADFASCLAGLREAAAASGI
                     SARTVSAALSNISPDMKIIEAMNNQPEFKTPIWDYLGTLVDDEKIAEGKAMLRRHAAT
                     LAAAEERYGVDRHAIVAVWGVESDFGKAGGKWPLPQALSTAACLAPRRNAFFKGELIA
                     ALKIVDRGDIPLESLVGSWAGAFGHTQFIPTTYLRLAVDGDGDGRRNLVSSIPDALHS
                     TANFLAKSGWQSGAGWGFEVRVPAGYGGASGRNPKQPIAAWAGRGITRIDGSALSGGG
                     SAGLLMPAGRNGPAFLVFRNYDAAYAYNGADSYALAISLLSDRLRGKGGIQAPWPTDD
                     LPLSREERRELQRLLIARGFDVGEPDGAVGAKTRAAIKSVEAEIGMPQTGRPGSKVLR
                     ALRGR"
     gene            2570..3679
                     /gene="leuB"
                     /locus_tag="PLQ11_00015"
     CDS             2570..3679
                     /gene="leuB"
                     /locus_tag="PLQ11_00015"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HRK23314.1"
                     /translation="MASYNLLLLPGDGIGPEVMVEVKKVLSFFKKAGIARFETETDLV
                     GGSAYDAHKVAITDQAMKAAQQADAVLFGAVGGPKWANVPYEARPEAGLLRLRKDLGL
                     FANLRPAVCYPALADASSLKRDVVEGLDILIVRELTGGVYFGEPKEIVTLENGQKRAV
                     DTQVYTTGEIERIARVAFELARTRRNKVSSAEKHNVMRSGVLWKDVVTGLHKADYQDV
                     ELEHVLADNCAMQLVRWPKQYDVLVTDNLFGDVLSDIAAMLTGSLGMLPSASLGAPVS
                     RTGKRKSLYEPVHGSAPDIAGKGLANPIAMIGSFGMALRYSLGLGEAADLVEKAIADV
                     LASGLRTRDIAAQGQNTIGTGEMGDAIVKALQRLA"
     gene            complement(3759..4046)
                     /locus_tag="PLQ11_00020"
     CDS             complement(3759..4046)
                     /locus_tag="PLQ11_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23315.1"
                     /translation="MRIALLSAALVATSLISAPLAARDRGLVIDVKPRSWLDAGNVAR
                     AGQGRDYVTNTQAFGGAPMHGISSRSESNLPTRGLSGSGFAFEFLGANAVR"
     gene            complement(4132..5322)
                     /locus_tag="PLQ11_00025"
     CDS             complement(4132..5322)
                     /locus_tag="PLQ11_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23316.1"
                     /translation="MSFVTEGRTGIRSEISGFVVHEDQSLEARLRRVYSSARQAAGDR
                     LAKAQDATAAPATRLSPLWERAGALGAAASDLASAKLNRSAKIISSALQAIAHRCLDR
                     SRLFTRLKDSGALVWLVVKVVSRLLWRVPRAVKLIGLTLIAVSAAMLWSDEKDGPSPA
                     QAVAMKPAEVEEARPRPDAWKAVASPIASYHLEAPELDRSTLAYRARTHRDGPRQDIL
                     AWTAQADSTGRSRARMSGTLFVESYGRLRPERETLFIDLSRRAALTGASIERLGTTIG
                     LKTKFGALETAEIALSSDGRAARGCLAFRHSTSEAVLDLHGWFCGTADRPVDRAELAC
                     LIDRIDLVRAGNDTRLKSYFAEVERARQPCGAARLVTIASNWMDGDDAPVLKAGFSSM
                     AGLR"
     gene            5458..6492
                     /locus_tag="PLQ11_00030"
     CDS             5458..6492
                     /locus_tag="PLQ11_00030"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HRK23317.1"
                     /translation="MGYKVAIAGATGNVGREMLNILSERAFPVSEVVALASQRSVGTE
                     VSFGDKTLKVKALEHYDFSDTDICLMSAGGEISKQWSPKIGAQGCVVIDNSSAWRYDA
                     DVPLIVPEVNADAVRGFAKKNIIANPNCSTAQLVVALKPLHERFGIKRVVVSTYQSVS
                     GAGKDGMDELFEQTKAIYSAAEPKTKKFPKRIAFNLIPHIDVFMEDGFTKEEWKMTAE
                     TKKILDPKIKLTATCVRVPVFIGHSEAVNIECQKPITADEAREVLRTAPGVLVIDKRE
                     PGGYITPHEAAGEDATYISRIREDATVENGLAFWCVSDNLRKGAALNAVQIAEVLVNR
                     KLLKPRSKAA"
     gene            6658..7662
                     /locus_tag="PLQ11_00035"
     CDS             6658..7662
                     /locus_tag="PLQ11_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23318.1"
                     /translation="MSHESLPSPARSRVERAILRLQGGGLAGAILAFLLIKLAGLGIT
                     LLWPLKSAKFAPLVLLCCAEVSWLFGGTVGLLVVLQWLVHELAHLHVSRRFGHMPGLR
                     SLTPFVGVYLGTTAAQWPSREAEATVAIAGVLAGLCLAFALHVAGSMSGDEAMVLAAR
                     LGFMMTLIHLLPFRPFDGGRIFPGLFWSIRSTTDPAEPMRMGSTTIRLCGWLVGLIPA
                     PVRRRLAVTGSVLGDGLVAALAWATVIVTLSGLALPAGAFMAIMLAAIIGLVALFAAV
                     LSIDPRHGPAASRRLLPRNGQVTVETSARPLPWTDRSAPRAAFCIYAAALLAAIAGSF
                     "
     gene            complement(7673..8326)
                     /locus_tag="PLQ11_00040"
     CDS             complement(7673..8326)
                     /locus_tag="PLQ11_00040"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HRK23319.1"
                     /translation="MAANADLPKGGRHGLPPRLVEGYEAFKANRLPREHQRFVEMARD
                     GQKPETMIIACCDSRVSPEVIFDANPGELFVVRNVANLVPPFEPMAGLHGVSAALEFA
                     VQALKIRNIVVMGHGRCGGVHAFVKRLRRPQQSDVLSPGDFIGKWIELIAPAAESLPA
                     QDDESDHDYAERLARTSIRNSIENLLTFPCISTLVNRGKMEIYGCIFDVTTGRLETL"
     gene            8433..9302
                     /locus_tag="PLQ11_00045"
     CDS             8433..9302
                     /locus_tag="PLQ11_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HRK23320.1"
                     /translation="MSFRPRRSALYVPANNVRAIEKAITLPADVIILDLEDAVPPQAK
                     NEARRNAASAIRSQAFRDKELVLRINGLDSDEAAEDLVVGAGSDAVLVPKVQDANDIH
                     RAEAGLNEIAGKDRPALWAMIETPKAILNIAAIAAVAARDDARLACFVLGTNDLAKDT
                     RVNLTGSRLAMLSWISQTVLAARAYDLAVLDGVYNNLKDPDGYLAECRQGRGFGFDGK
                     TVIHPSQIADANHIFGPTDSEIAEARAIVEAFARPENAGKGVLTVGGRMAELLHRDQA
                     ERTLAIARATGLA"
     gene            complement(9329..9865)
                     /gene="ppa"
                     /locus_tag="PLQ11_00050"
     CDS             complement(9329..9865)
                     /gene="ppa"
                     /locus_tag="PLQ11_00050"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020372439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HRK23321.1"
                     /translation="MRIDAIEIGKNPPFDVNVIVEVPIGGEPIKYELDKASGTLVVDR
                     FLYTPMHYPGNYGFIPHTLSDDGDPCDVLIANTRPIVPGALVNVRPVGVLKMQDDGGG
                     DEKIIAVPSPKLTKRYEHVHNYTDLPTITWKQIEHFFTHYKDLEPGKWVKLMGWGDAD
                     EARKLIVDGIERYKASSK"
     gene            10245..10967
                     /locus_tag="PLQ11_00055"
     CDS             10245..10967
                     /locus_tag="PLQ11_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010687123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur transferase domain-containing protein"
                     /protein_id="HRK23322.1"
                     /translation="MVFAIFRPERRYARRLARAARWDRPIAGRWQRFCAWTNMLLIDH
                     GLFRLIYLNAHPVTDDLWRSAQPSPHQVADFAARGIRTIVNLRGGREFGSWPLEREAC
                     DRAGITLVDFVARSREAPGIDTVLAAQKLFDSIEYPALIHCKSGADRAGFVSALYLML
                     KHGQPASLALEQLSAGYGHWRWSKTGILDAFIERYRDQGEARGLSLIDWVTTEYDPDE
                     LTRSFRPHAFSAFIVDRILRRE"
     gene            complement(10954..12759)
                     /locus_tag="PLQ11_00060"
     CDS             complement(10954..12759)
                     /locus_tag="PLQ11_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRK23323.1"
                     /translation="MTDASASAPPDPPAKLLHDRQRLIPAVRRLWQGWVRPHGGTILI
                     IWLTVLVIAAMSSMYPYIIKRAFDAFDAKDEAFLKLTPVLVIMVTAMRGFAMFAQLIL
                     TNRVVSKVEADLQTSLFHSLIDADVAQLSRETPASLTQRFTTDFAYVREALTRLSTVL
                     ARDVATAVAVLAVMVWIDWMITLAALMVLPIIVPPIERIGKKLRRTALSTQEQTGIMA
                     SLVAESLGGVRIAKTYGLEPYLKSKAADAFDAVRRLKVKAANQRARLDPLLEVGGGVT
                     VAAVLVFIGWRIAQGQSTIGDFTGYVTAVLLAAQSLRTLGNLNAILHEAVAALTRIFA
                     VIDERPQVEDAVDGPELVVKQGEVAFAGVSFSYANSGSALSDVSLVVPAGKTTAFVGR
                     SGAGKSSLLALVPRLFDPTAGVVTIDGTDIRSVSLKSLRAHLAVVSQDIVLFDDTIRA
                     NIAFGRQRASETEIIAAASAAAAHGFIAALPDGYDTRVGDRGGRLSGGERQRVALARA
                     FLRNAPILLLDEPTSALDAQSEDLVQKALGDLMRGRTTLVVAHRLATVRAADLIVVMD
                     EGRIVETGTHDSLMASDGAYAAFYRLQFGEGVTPA"
     gene            complement(12842..12916)
                     /locus_tag="PLQ11_00065"
     tRNA            complement(12842..12916)
                     /locus_tag="PLQ11_00065"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12882..12884),aa:Gly,seq:gcc)
     gene            13074..13562
                     /locus_tag="PLQ11_00070"
     CDS             13074..13562
                     /locus_tag="PLQ11_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HRK23324.1"
                     /translation="MDRLAIAHRTLQHVAVYTVAESRELRGTIPGAHSKNLFLKDKKG
                     RLFLVTALEDAPLNLKKLHEAIGGQGRVSFGSAEQLRACLGVEPGSVTPFAAMNDTDG
                     RVTVVLQRKLAEAPVLNFHPLTNTATTTITNQDLLQFLAAIGHPPHIVDLPEGEASSP
                     EA"
     gene            13641..14525
                     /gene="trxA"
                     /locus_tag="PLQ11_00075"
     CDS             13641..14525
                     /gene="trxA"
                     /locus_tag="PLQ11_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRK23325.1"
                     /translation="MLGAQPEGLIKDTTTQGFRADVIAESARQPVLVDFWAPWCGPCK
                     QLTPVIEKAVRAARGKVRLVKMNIDDHPEVAGQLGIQSIPAVIAFSNGKPVDGFMGAL
                     PESKVTAFIERLVGPSADDPATLIKLAEERKAGGDIGGAAEAYAVVLAKDPHHGAALA
                     GLAGLQMDTDNLEQARRILDSVPEAKRTEPAIVAAIARLELMERAASLGDLADLERRI
                     ASNPADHQARFDLAVALNAKGFRAEAADHLLDIIKMDRSWNDDGARKQLLQLFEAWGP
                     MDEVTLGARRRLSAYLFR"
     gene            14562..15230
                     /locus_tag="PLQ11_00080"
     CDS             14562..15230
                     /locus_tag="PLQ11_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HRK23326.1"
                     /translation="MPSNVQYQGPAQLPEVIPIFPLAGALLLPRGQMPLNIFEPRYLA
                     MVDAALAGDRLIGMIQPINEKAGEAPPDLMAVGCVGRLTEFAETGDGRYLITLTGIAR
                     FRLIEEVVTDAPFRKAQVSTAPFRPDFIARLGEDAVDREAVLIALRAFSDAKAVEIDW
                     ESVGQASNEALVNALSMMCPYGLKEKQALLEAPDLKTRAELLIALTEIDLSGPGGDGD
                     ITVQ"
     gene            15236..15445
                     /locus_tag="PLQ11_00085"
     CDS             15236..15445
                     /locus_tag="PLQ11_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019965359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HRK23327.1"
                     /translation="MTGSNAPERYATRSIDPKFLELLVCPLTKTALDYDAVRQELVSH
                     AAKLAFPIRDGIPIMLVDEARRLAD"
     gene            complement(15454..16677)
                     /locus_tag="PLQ11_00090"
     CDS             complement(15454..16677)
                     /locus_tag="PLQ11_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018261306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HRK23328.1"
                     /translation="MAVIDDVDVVIAGGGAAGLALALALNVAAGSALKVAVCDPELAR
                     DRRGDSRAYAIVAGAIRFFDSIGAWQAMRDEAQPILRMEITDSRLEDLVRMPLLTFGA
                     ATQDEAPPAMMLANGTILAALMARARSAGLSLFSEPVSGFDQTGGRIAAIVGGRRLRA
                     RLLVIADGRGSRLRERAGIAYHGWRYPQTAIVGTIHHTLDHEGVAVQHFLPAGPFALL
                     PLKGRRCSIVWNERPETVERLLRCDSDDLVTEIDRRAAGRFGTVTRVEGLASFPMSIG
                     IARRFVGPRLALLGDAAHGFHPIAGQGLNYGLRGAAALAETIIDAARLGLDIGSETAL
                     GPYEAARRPDVMAMAAATETLNRLFSNDFGPLRAIRDLGLSLVDRLPGLGARFMQEAA
                     GDSSRAPRAFRGLPI"
     gene            16747..17604
                     /gene="tesB"
                     /locus_tag="PLQ11_00095"
     CDS             16747..17604
                     /gene="tesB"
                     /locus_tag="PLQ11_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HRK23329.1"
                     /translation="MSVISDLLTILDLEPLEQTLFRGRVPQVGWQRVFGGLVISQALV
                     AAHRTVDGREPHSLHGYFLLGGDPSVPIIYDVDRIRDGRSFTTRRVVAIQHGRPIFAM
                     SCSFHEPEDGFDHQATMPDVPMPEDLPSIETVKKSVLPNLPHPVRAYFERERPIELRP
                     VDFERYTASGSAQLGTQIWIRAAGRLPDDPGIHQSVLAYASDMTLLDCSLVPHNTSVF
                     DPGIAPASLDHAMWFHRPFRADEWLLYSQESPNACAGRGFSRGLVFRRDGTLVASVAQ
                     EGLIRRKRP"
     gene            17818..19131
                     /locus_tag="PLQ11_00100"
     CDS             17818..19131
                     /locus_tag="PLQ11_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HRK23330.1"
                     /translation="MNPRLFSASALLMSAAVVDPALAQSTSKIDAADTGFMIVCTALV
                     LMMTLPGLALFYAGMVRRKTVLATMAQSAASIAIVSLLWIAVGYSLTFSGDGPIFGDL
                     SRAFLLGIKPDTTTALAPTIPELLFMLYQMTFAIITCALIGGSVADRMRFSAFLIFSV
                     LWLFLVYVPSAHMVWGNGWLAKLGVIDFAGGTVVHINAGIAGLVAALMIGPRHGYGRD
                     NFAPYDLSMAVVGTGLLWVGWFGFNGGSALGAGSRAVYAITATHLAACAGAVVWTGLE
                     WIQRGRPSVLGIISGAIAGLGTVTPASGFILPWHGVVIGVIAGALCYWACIWLKHKLA
                     YDDSLDVFGVHGVGGLLGTMLAGVFATGSLSATPELAEGLRGLVDGNPGQVVTQAIGV
                     VVTTLWCAAGTFVALKVTAFMTPLRASRDEEVEGLDLALHGERLH"
     gene            19377..19715
                     /locus_tag="PLQ11_00105"
     CDS             19377..19715
                     /locus_tag="PLQ11_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HRK23331.1"
                     /translation="MKTVTAIIKPFRLEEVRDSLTALGVHGMTVTEVKGYGRQKGHTE
                     IYRGAEYAVSFLPKLKLEVGVSDELVGPVVEAIAEAARTGQIGDGKIFVAPLDQVVRI
                     RTGERDGDAL"
     gene            19743..21215
                     /locus_tag="PLQ11_00110"
     CDS             19743..21215
                     /locus_tag="PLQ11_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HRK23332.1"
                     /translation="MSCKSIVLRLAAPCLVAGLTLAPAFADTPAPQPNKGDTAWMLIA
                     TALVLMMSVPGLALFYGGLVRAKNMLSVLTQVFAIVCLAGLMWVAVGYSLAFTNGGAL
                     NDFVGGFGKAFMAGITPASNAATFSNGVVIPEYLYLAFQMTFAMITPALIVGAFAERM
                     RFGAVMAFTALWSLLVYYPIAHMVWYWAGPDALADAAKAVAAATDAVAKTAAQAKLDE
                     AVADAGMIFKWGALDFAGGTVVHINAGIAGLVGCLMVGKRIGYGRELMAPHSLVMTMI
                     GAALLWVGWFGFNAGSSLEANGTAVLAFVNTMVATCAAALAWLIVEWLLKGKPSLLGM
                     VSGAVAGLVAVTPASGFAGPMGAIVLGFVSGAACFIFVAYVKGALGYDDSLDVFGIHC
                     IGGILGAIATGILASPDLGGAGIVDYVAKPGEAAPGDFVMATQLMAQLKAVAVTLLWS
                     GLVSMILFKLIDLAIGLRAESDDERMGLDIAEHGERAYSA"
     gene            21349..23856
                     /locus_tag="PLQ11_00115"
     CDS             21349..23856
                     /locus_tag="PLQ11_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HRK23333.1"
                     /translation="MRTTRLGSSATDYIPEAIRDGIMRRLAELAGLILLLAAGAGALA
                     LATWSVRDPSLSHAVAVPARNLLGYPGAVIADLLMQIFGLAAIALLLVMAIIGWRLMA
                     RRQLQRPALRLIVAMVAVICAAGLAGTLPRTDRWPLPGGLGGLVGDLLLYLPRLLTGS
                     LDMLLRLPVGFACAVGLIISIAYVGRRSSEEEAEEQARIAEEDVESSADDEPSVTIIS
                     LGAVIHALFSARSWIARRFALLMGRQRMSSEAPARRRTPASRRTEPTFEPTGPVQAPA
                     KRTRALPEPDFDSLDDTPFDDPQPVKAKVSAPVGALKPGRRAEREAQPSMFGQDGYQH
                     PPLTLLSEPKRAPGVIISKDALEQNARLLEGVLDDFGVRGEIINVRPGPVVTLYELEP
                     APGTKSSRVISLAEDIARSMSAVAARVAVVQGRNAIGIELPNAKRETVFLRELLACED
                     FEKSKMRLPLCLGKTIGGEPVIVELARMPHLLIAGTTGSGKSVAINTMILSLLYRLTP
                     DQCRLIMVDPKMLELSIYDGIPHLLTPVVTDPKKAVVALKWAVREMEERYRKMSKVGV
                     RNIDGFNARVAEARDKGEVIQRMVQTGFDRETGEPIFENEVMDLQPLPFIVVIVDEMA
                     DLMMVAGKEIEGLVQRLAQMARAAGIHVVLATQRPSVDVITGTIKANFPTRISFQVTS
                     KIDSRTILGEQGAEQLLGQGDMLYMAGGGRISRVHGPFVSDAEVEHVVAHLKAQGAPQ
                     YLDAITAEEESAEESDDGAIFDKGNFGEEPQDLYDKAVQVVLRDKKCSTSYIQRRLQI
                     GYNKAASLVERMEKEGLVGPANHAGKRELLLEGAEAG"
     gene            23964..24665
                     /locus_tag="PLQ11_00120"
     CDS             23964..24665
                     /locus_tag="PLQ11_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer-membrane lipoprotein carrier protein LolA"
                     /protein_id="HRK23334.1"
                     /translation="MKTTSFLAAGFALLLAIGGALAQLNLNPPAATTPGAPKGPAPAA
                     PAPAAAAGQNETPRPTVERVNAYFNAIGGMTADFVQIGPDGRRWEGKLSILRPGKMRF
                     EYKPPSPLEIISDGTTVAVRDRKANTQDEYFVGQTPLKFLLRDRIDVARDAKVISLAQ
                     QGSDAVLVIEDRQTVGGTSRIRLTFNNADMMLKQWIVTDPQGYDTRVQLANIDTTRRP
                     NAALFVIPTKVSTPN"
     gene            24775..25584
                     /gene="xth"
                     /locus_tag="PLQ11_00125"
     CDS             24775..25584
                     /gene="xth"
                     /locus_tag="PLQ11_00125"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012255182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HRK23335.1"
                     /translation="MTLTIATWNINSVRLRLDLVLRYLSEAAPDVLCLQETKCPDAEF
                     PRSAFVKAGYPHILHRGMKGYNGVAILSRLPLAAAENLTLCGREDARHIAAEVRPADG
                     PSLLVHNFYVPAGGDIPDRIENPKFGHKLDFLAEMTDWAERLRARHDRAVLVGDLNIA
                     PLVHDVWSHKALLKVVSHTPIECEALLHLQDSGGWIDAVRCLNPEPEKVYTWWSYRSP
                     DWNAADKGRRLDHIWVGPALKPAIASSSILRAARGWERPSDHVPLAVTLAL"
     gene            complement(25637..26074)
                     /locus_tag="PLQ11_00130"
     CDS             complement(25637..26074)
                     /locus_tag="PLQ11_00130"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRK23336.1"
                     /translation="MRLDEKTTALAGLALFRALEPEALHVLAFSATTQQLKAGEDLFR
                     KGARADTGYVVVSGRIGVQATDGAPGEGEVVGPGGLIGVSALLVAGERPGTALALEPS
                     RVLVLSQSLMRQVLEAHPASAAALRAHLAGELASTRQRLAEII"
     gene            complement(26086..26772)
                     /locus_tag="PLQ11_00135"
     CDS             complement(26086..26772)
                     /locus_tag="PLQ11_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRK23337.1"
                     /translation="MSNIRKILIVDDDAALREALAEQLHLHEEFAVQEADTATAALAI
                     AKADQIDMILMDVGLPDIDGREAVKMLRKNAFRGPILLLTGHDSEADTVLGLESGAND
                     YVTKPFRFAVLLARIRAHLRQHAASEDAVFAIGPYTFRPGAKLLVTTKGSKLRLTEKE
                     TAILRYLYRADQKVVPRDVLLQEVWGYNSNVTTHTLETHIYRLRQKIEPNSGHARLLV
                     TEGGGYKLVP"
     gene            26849..27376
                     /locus_tag="PLQ11_00140"
     CDS             26849..27376
                     /locus_tag="PLQ11_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRK23338.1"
                     /translation="MKGYCTTIVVYRLEPRGPRALLVAGPLAMPCRIGRNGAACAKRE
                     GDGASPLGTFALLGGWWRNDRRRRPQTGLCLTAIRKDDGWCDAPGHRAYNRPVRLPFA
                     ASHESMARNDHQYDVVLDISWNRHPRRQGWGSAIFLHLMSETGGGTAGCVAVPAGRID
                     RLLVRIGPQTRIRIR"
     gene            complement(27386..28066)
                     /locus_tag="PLQ11_00145"
     CDS             complement(27386..28066)
                     /locus_tag="PLQ11_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HRK23339.1"
                     /translation="MGESQEDHPGAVRIDAVRKLIRRAAEDCGRPAADVTLIAVSKTF
                     PVEAIEPVLLAGQRVFGENRVQEAQQKWPQLKQRFPDVDLHLIGPLQTNKAETAVTLF
                     DCIESLDRPRLAAALAAAIARTGHSPRLFVQVNTGAEAQKAGVLPEEADAFIAECRVK
                     HGLTIAGLMCIPPADQPPSPHFALLAKIARRNGIDQLSMGMSADFAAAIQSGATHVRI
                     GSAIFGAR"
     gene            28186..30786
                     /gene="leuS"
                     /locus_tag="PLQ11_00150"
     CDS             28186..30786
                     /gene="leuS"
                     /locus_tag="PLQ11_00150"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HRK23340.1"
                     /translation="MRNARYNPREAEPRWQKIWNDRNTFRTRNDDSRPKYYVLEMFPY
                     PSGRIHMGHVRNYAMGDVVARHKRAKGYCVLHPMGWDAFGMPAENAAMEKKSHPKAWT
                     YANIATMKGQLKSMGLSLDWSREIATCDPAYYRHQQAMFIDFLKAGLVARKTSKVNWD
                     PVDHTVLANEQVIDGRGWRSGALVEQRELTQWFLKITDYSQDLLDALAKLDRWPEKVR
                     LMQQNWIGRSEGLLIRWALDKAPAGIDAVEVFTTRPDTLFGASFIAVAADHPLARAAA
                     EKDARLAAFIEECQRMGTSVAVLETAEKKGYDTGIRAVHPFDSSWTVPVYVANFILMD
                     YGTGAIFGCPAHDQRDLDFANAYGLAVTPVVRDRALSDAEANAQFDRHAPAAVDDGIL
                     FNSRFLDGLTVDAAFNEVADRLEASRLAGEPVGRRKVNFRLRDWGISRQRYWGCPIPV
                     IHCEACGIVPVPKKDLPVELPEDVTFDVPGNPLDRHPTWKRVACPTCGRLARRETDTM
                     DTFVDSSWYFARFTDPFNGTSPTTRAVVDRWLPVDQYIGGIEHAILHLLYSRFFTRAM
                     RKTGHAGIDEPFAGLFTQGMVVHETYKSADGAWVEPAHVEIAAGPAGRTARDRRNGAA
                     ITIGSIEKMSKSKKNVVDPDDIIETYGADTARWFMLSDSPPERDVIWSEEGVQGAGRF
                     VQKIWRLICEIADRPADRLADRMGSALLRTAHKTAKAVDDDIERLRFNRCVAHIHDLS
                     NALATALGEGSETTTALREACEIMVRLFAPMMPHLAEECWEVLGHATPVSEAPFPVID
                     AELIIDLSITLPVQVNGKKRADVTVPADADRAAVETATLALEAVQRALEGRAPKKIIV
                     VPKRIVNIVV"
     gene            30798..31301
                     /gene="lptE"
                     /locus_tag="PLQ11_00155"
     CDS             30798..31301
                     /gene="lptE"
                     /locus_tag="PLQ11_00155"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HRK23341.1"
                     /translation="MVSRRFPLAALVLVLPLAGCFQPLYGPGLAGSTEAIGSFQIESM
                     PEHLGHQLKSELEFQLNNGTPPSKARFRLVAQPSGRSAGVIADTASSRPQVMSYIASA
                     RFSLHEIKTGKVITSGQAQVQASYDRDVQRFATVRALRDVEIRAAIQLAEQIKIRIMP
                     VVAGLKD"
     gene            31305..32345
                     /gene="holA"
                     /locus_tag="PLQ11_00160"
     CDS             31305..32345
                     /gene="holA"
                     /locus_tag="PLQ11_00160"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HRK23342.1"
                     /translation="MTAIKSGDLARFMAGGWQTCPVVLVFGPDEGAVREIAGQLVRAA
                     AGADPDPLNCIRLDGETLAQDPARLADEARTFGLFGGRRVVHVRNAARTPAASVTAIA
                     DDPVPDTLVILEAVDLKSGAPLRTLAEKHRSIAAIACYADGHRSLQTLIETTLSGHGL
                     SLTRDGRDALMGALGADRLLTRSELDKLTLYALGEKTVDARMVAEIVSDAGRHETSSL
                     IDFAFAGHLATVEPEANRIFATGIHPASLMTQVLSHIMLMRRLRRAVDRGQSLDALLR
                     QARLHFSRVPAVEAALRSWNCERLDRALVLASDAVLNSRRTARLADSLAIRLLWSIAR
                     MAGKDKVSSRIQ"
     gene            complement(32540..33409)
                     /locus_tag="PLQ11_00165"
     CDS             complement(32540..33409)
                     /locus_tag="PLQ11_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018262195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRK23343.1"
                     /translation="MVDETHRQRLGRGLAALIGDVGGEERSERMPRRASVAFLRANPN
                     NPRRTFTELEIEELTASVREKGILQPILVRAISGVPDQFEIIAGERRWRAAQRAGLHE
                     VPILVVEADDRQALELAIIENVQRADLNPLEEARGYESLLTQFGYTQADLSRVVGKSR
                     SHVANTLRLSRLPDSVKSLLSEGKLSAGHGRALLAVADPEGIAKRVLAQGLTVRDVER
                     LAQREVEDAETGVSPRPRPEKDADTRALEKALGDILGLPVSIDHRREGGALTVRYRSL
                     EQLDALCRRLRGA"
     gene            complement(33436..34233)
                     /locus_tag="PLQ11_00170"
     CDS             complement(33436..34233)
                     /locus_tag="PLQ11_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HRK23344.1"
                     /translation="MTQARILAIANQKGGVGKTTTTINLGTALAAIGENVLIIDLDPQ
                     GNASTGMGISQKERRQSTYDVLVGEITLGEAIHATAVPRVSLAPSTLDLLGAELELAS
                     RKDRALRLREAIAEHQSARAEPFSYILIDCPPSFNLLTINAMAAADAVLVPLQCEFFA
                     LEGLSQLLKTVDQVRQGLNPRLIIHGIVLTMFDPRNNLANQVISDVRQFLGDKVYQTV
                     IPRNVRVSEAPSHGKPVLLYDLKCAGSQAYLKLASEVIQRERMLRAA"
     gene            complement(34249..34926)
                     /gene="rsmG"
                     /locus_tag="PLQ11_00175"
     CDS             complement(34249..34926)
                     /gene="rsmG"
                     /locus_tag="PLQ11_00175"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HRK23345.1"
                     /translation="MNGQGDAVDRDSSGNRERFRALVDVSRETLEALDRYEYLLRRWQ
                     GSINLVGKTTLSEVWTRHFLDSLQIADLVPRARTFMDLGSGAGFPGLVIAIRLKERGI
                     GRITLIDSSAKKTAFLTKVQQTLELPAEVVTGRLEQWLPRLPVPEIVTARALAPLTQL
                     LDWCEPLLKSGSRALFLKGRNLDNELSEAAQYWEIDFVRHAHALEAESWILEVRSARR
                     RTPPSGT"
     gene            complement(34923..36833)
                     /gene="mnmG"
                     /locus_tag="PLQ11_00180"
     CDS             complement(34923..36833)
                     /gene="mnmG"
                     /locus_tag="PLQ11_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006468041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HRK23346.1"
                     /translation="MSTNQTFDVLVVGGGHAGTEAAAAAARMGAATALVTLRRDNLGV
                     MSCNPAIGGLGKGQLVREIDALDGLMGRIADAAGIQFRVLNRRKGPAVQGLRAQADRR
                     RYQRAMTEAVAAIDGLTVIEGEVADLVISDGVAAGVVLSDGRLLRCGAVVITTGTFLR
                     GTIHVGQSRQSGGRIGEEAANALSRRFEHLGLKLGRLKTGTPPRLDRGSIAWDRLELQ
                     HGDAEPVPFSFLTPSISGEQVACGITRTGADTHALIQGNLGRSAMYSGQISGRGPRYC
                     PSIEDKIVRFGDREGHQIFLEPEGICSDLVYPNGLSTSLPEDVQAAMIASIPGLERAV
                     IRQFGYAIEYDFIDPRELTADLQCKRLPRLFLAGQINGTTGYEEAAAQGVVAGLNAAL
                     VAGGRDSAVLEREGSYIGVMIDDLVTRGVTEPYRVFTSRAEFRLSLRSDNADRRLTPI
                     GLSLGCVGKQRKEAFEIWAQKFDSATKVLEACTITPNAARAAGLDIKQDGVRRSAYDL
                     LSLPGIDIARLTDVWPELHQIECDVREAVSVDARYAVYLGRQKQELERIERDRAIAIP
                     DALDYRGLPGLSSELASKLTMIRPRSIEQASRIEGMTPAALALVCAHARRWEPSRFTG
                     PSSGGCGHEGRQ"
     gene            complement(36929..38239)
                     /gene="mnmE"
                     /locus_tag="PLQ11_00185"
     CDS             complement(36929..38239)
                     /gene="mnmE"
                     /locus_tag="PLQ11_00185"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HRK23347.1"
                     /translation="MDRADTIFALSSGQGRSAVAIVRLSGPQVRFALETLCGSVPSVQ
                     SARLRSIRDPKTGGVLDRGIVLFFSGPNSQTGEDVGELHCHGGPAVVAAMLRALGSLP
                     GLRMAEPGEFARRAVFNGKMDLLDLEGLADLIDAETERQRVQAIEGGGSALRDVVEGW
                     RCSLIEVMAAVAVEIDFSDEGDVAERLDSDMERAVDALIGELKRALATAAGGERIRGG
                     VRVAIIGRPNAGKSSVLNALARRDVAIVSEVPGTTRDRIDIHLDLDGIPVIVSDTAGL
                     RDSADKIEQEGNRRSRQAAREADLVLWLQPVDDVRELPVEFRGDVRVVASKADLGEAP
                     FSAWCRVSTVAPGGCDELLGALKRAVEVLVGRTDVALVTRTRQRLRIEAALACLGRAR
                     QAGLGIEFVAEDLRQASVELDRLVGRIEVDDVLGAIYSRFCIGK"
     gene            38303..40318
                     /locus_tag="PLQ11_00190"
     CDS             38303..40318
                     /locus_tag="PLQ11_00190"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HRK23348.1"
                     /translation="MAPNRLAQALSPYLLQHSGNPVDWWPWRTDAFELARQTQRPVFL
                     SIGYAACHWCHVMAHESFEDPETAALMNELFVNVKVDREERPDIDQIYMAALHALGQQ
                     GGWPLSMFLTPDGQPFWGGTYFPKTRRYGQPAFSEVLRSVARAYHANPDTIRNNTEAL
                     IASLAAAPAEAGTIPPPDRLVTIARAIAGHFDPLHGGLAGAPKFPNAPVLDLISRAAT
                     ASKDPALLTPVLTTLERMVRGGLFDQIGGGFHRYSVDAAWLVPHFEKMLYDTAQLLPI
                     LAMTGRSTASELLLKAADRCVEWLKRDMIAPQGGFISSLDADSLTPDGHREEGAYYTW
                     TPDEVTAVLGPAAAPLCKAFDIMSPGNFDGRSIPNRLGRTGLHPPASDNHDVALDALL
                     AARLLRPPPPRDDKVLADWNGMMITGLARAADKLDHPDWRKLATSAYRATVELLEPNG
                     NLRHVWRGKPSVGPCFASDLASMALAATALHESTGDLNYIPDAERWLTTLITDYRLPS
                     GRLALTARSAEPLIHRPQSTQDDAVPNHHALAIEAMIRLGAQTGDLFWHQRASSLMSA
                     VSEAMLDNPYGHASLLSMVDFATRRCDILVAGQIDAAFAAAIRAVPYPNRTIRFLTAA
                     QAAAHPLTAALPCPDQPNVLICLADRCLPMATTLPEFRDRLATAGWL"
     gene            complement(40319..41590)
                     /gene="rho"
                     /locus_tag="PLQ11_00195"
     CDS             complement(40319..41590)
                     /gene="rho"
                     /locus_tag="PLQ11_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008191409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HRK23349.1"
                     /translation="MREIKLQELKAKSPTELLTFAEEVEVEGASTMRKQELMFAILKQ
                     LAARETEIIGEGVVEVLQDGFGFLRSPDANYLAGPDDIYISPSQIRRFALRTGDTVEG
                     QIRSPRDGERYFALLKVNKINFEDPEKARHKVHFDNLTPLYPEQRLKLEIEDPTKKDM
                     SARVIDIVSPIGKGQRALIVAPPRTGKTVLLQNIAKSITHNHPECYLIVLLIDERPEE
                     VTDMQRSVKGEVISSTFDEPATRHVAVAEMVIEKAKRLVEHGRDVVILLDSITRLGRA
                     YNTVVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTIISTALIDTGSRMDEVI
                     FEEFKGTGNSEIILDRKVSDKRVFPAIDITRSGTRKEELLVPQDILKRMYVLRRILNP
                     MGTVDAIEFLLDKLTKTKQNSEFFDSMNTRD"
     gene            41768..42631
                     /locus_tag="PLQ11_00200"
     CDS             41768..42631
                     /locus_tag="PLQ11_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HRK23350.1"
                     /translation="MQPFTPTQLPHPSNLSKPIFAYLQRLSCKARIRLPFPPQNLPLN
                     SPFVADNHTNLGSPPMRSKGSSLVLVTCALFAGNALAQQQGPQPNAGMNFFISSASLG
                     KGGDLGGLEGADRHCQTLATAVGQGSKTWRAYLSTQAADGKPAVNARDRIGKGPYLNF
                     KGEEIAKDIAGLHDPAANRINIETGLTERGQKVMGRGFLINMHDVLTGSQTDGTAFPP
                     EKDMTCGNWTKSGAGAAMVGHHDRIGLDDSAPAKSWNASHPSRGSEGGCSMADLRSTG
                     GGGLIYCFAAN"
     gene            complement(42696..43136)
                     /gene="hemJ"
                     /locus_tag="PLQ11_00205"
     CDS             complement(42696..43136)
                     /gene="hemJ"
                     /locus_tag="PLQ11_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018430514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase HemJ"
                     /protein_id="HRK23351.1"
                     /translation="MTVDGAYLWVKSLHVIAVISWMAGMLYLPRLFVYHVDAPKGSQQ
                     SETFKVMERRLLRAIINPAMIVSWLTGLWMAWYVFAFKGGWLHAKLAIVFVLLSGFHG
                     FLARWRKDFEADRNVRTAYFYRVMNEVPTVLMIAVVILVIVKPF"
     gene            complement(43133..44191)
                     /gene="hemE"
                     /locus_tag="PLQ11_00210"
     CDS             complement(43133..44191)
                     /gene="hemE"
                     /locus_tag="PLQ11_00210"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HRK23352.1"
                     /translation="MSSFVFERAHRRGPFLDALKGIPGSRPPMWLMRQAGRYLPEYRA
                     IRAKAPSFLDFCYSPDMAAEATLQPIRRFGFDAAILFSDILVIPDALGQTVRFVEGEG
                     PRLDPIRAAGGSAELKSSVDLERLAPVFETVRRVRAGLEPDKALIGFCGAPFTVATYM
                     IAGRGTPDQKPARMFAYKDPEAFHRLIDLLVDASADYLVAQFKAGVDCVQIFDSWAGV
                     LPRWEFDRWCLQPVEALVKKVRARVPGAPIIGFPRGASTELREFVERTGVDAVGVDTA
                     VDLGAVARSLDPAVTTQGNLDPFALIAGGSALDRAIEAILEAVDGRAHIFNLGHGILP
                     ETPIAHVERLVQKVRGEL"
     gene            44559..45389
                     /locus_tag="PLQ11_00215"
     CDS             44559..45389
                     /locus_tag="PLQ11_00215"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018262437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HRK23353.1"
                     /translation="MGRSFFHLHLVSDSTGETLLAVSRAASAQYQGISAIEHIYPLVR
                     NSNQLDRVLQQIDLTPGIVLYTLVDRSIAERLEHACRESGCPCLSVLEPVHSLFQSYL
                     GIGTTPRPGAQHMLNAEYFRRIDALNFTMMHDDGQLTDGIEQADVILVGVSRTSKTPT
                     SIYLANRGIKTANVPMVPGIPLPDILSSLERPLIVGLVASPERIVQIRQNRLLSLNAG
                     DDTAYVDRSSVAEELAYSRRLFDKYNWPIIDVSRRSIEETAAAILDLYHKRREQMIPE
                     "
     gene            45386..46009
                     /locus_tag="PLQ11_00220"
     CDS             45386..46009
                     /locus_tag="PLQ11_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003597702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HRK23354.1"
                     /translation="MNTAPGSLWQADAPLVLASASAVRRALLEAVGVPFVAQPARIDE
                     RALEAASKSRDPVELARLLADAKALAVSQDMPNRYVLGADQTLSIAGGMLHKPGSRRA
                     AVDQLMRLSGARHRLHAGLALAINGHVIWHHVETASLTMRPLSAAFVHAYLEAAGDPV
                     LASVGVYQIEALGMHLFEAIEGDHSTIMGLPLMPLLGRLRMMGLVLE"
     gene            46015..46638
                     /gene="coaE"
                     /locus_tag="PLQ11_00225"
     CDS             46015..46638
                     /gene="coaE"
                     /locus_tag="PLQ11_00225"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113853.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HRK23355.1"
                     /translation="MLVLGLTGSIGMGKSTTATLFGAAGVPVHDADATVHKLYRGRAA
                     PLIEARFPGSTNSGVVQRDRLAAAVLGRPAEIEALEAIVHPLVREEEIAFLTAARARA
                     ARLVVLDIPLLFETGAEARVDAILVVTAPDSVQKARVLARPGMTADKFAAILARQIPD
                     KEKRRRSHALIDTSFGLDAARRDVEALLRALAGHPGRRLVGPAVTSG"
     gene            46647..47366
                     /gene="dnaQ"
                     /locus_tag="PLQ11_00230"
     CDS             46647..47366
                     /gene="dnaQ"
                     /locus_tag="PLQ11_00230"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HRK23356.1"
                     /translation="MREIILDTETTGLDPRSGHRIVEIGCVECIDQFLTGRTFHHYVN
                     PERSMPPDAFAVHGLSDGFLKDKPVFADIADAFVEFLGEARLVIHNAGFDLGFLNHEL
                     ARCGRPLIGSDRIVDTLLLARRKHPGQSNSLDALCQRYGIDNSRRTKHGALMDAEILA
                     EVYAELSGGRQRSLGLAIAETVETNVVAIVPGQAYQPPPPLLGEAERAAHRALVESLG
                     GKALWRDYLGLDANQDRPPKA"
     gene            complement(47369..47863)
                     /gene="secB"
                     /locus_tag="PLQ11_00235"
     CDS             complement(47369..47863)
                     /gene="secB"
                     /locus_tag="PLQ11_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HRK23357.1"
                     /translation="MSQTPNAGQTEDTAPQLNALAQYIKDLSFENPRAPRSLTNQDGS
                     APNIQIQVNVNAQPIGGDDYEVELKLEGSAGTKPNLLFSFELNYGGVFRLRNIPQEHI
                     GGVLMVECPRLLFPFARQIVANAVRDGGFPPLYMDPIDFLALYQQRVAELQSQQGQPP
                     VKPS"
     gene            complement(47919..48392)
                     /locus_tag="PLQ11_00240"
     CDS             complement(47919..48392)
                     /locus_tag="PLQ11_00240"
                     /inference="COORDINATES: protein motif:HMM:NF016102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HRK23358.1"
                     /translation="MRIVRWSGSKIGWALILWFVVEVVLFWLATGTFGVLPTLAALAL
                     KGILGGMLLLAHLRSVLSGLPKAVLTRGLGGLGSTGFAALGALLIFLPGMLTTVAGLA
                     LFSPSVRNWVMNRFRRKQHASGAEGTITLEPNEWREVSSRSRRRAPAVQSGKVLP"
     gene            48507..49235
                     /locus_tag="PLQ11_00245"
     CDS             48507..49235
                     /locus_tag="PLQ11_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44/TimA family putative adaptor protein"
                     /protein_id="HRK23359.1"
                     /translation="MNDFLDIYTIVFIALAIFVILRLKSVLGERTGNERPPFDPFQRP
                     MPPKPDDGGNVVPLPTAARPAPAPAPIPAAVAAEAPADKLKGIAEPSSELGTNLIRVM
                     DAEPLFEPKSFLMGARAAYEMIVLAFARGDRRSLKDLLSKDVFEGFSAAISEREARGE
                     KVETTFVSIDKSDLTDASFKGGTVSVTVRFVSKMITATRDAAGAVIDGNPEKIVDLVD
                     VWTFSREAGARDPNWRLVSTQSDA"
     gene            49289..50404
                     /locus_tag="PLQ11_00250"
     CDS             49289..50404
                     /locus_tag="PLQ11_00250"
                     /inference="COORDINATES: protein motif:HMM:NF015523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HRK23360.1"
                     /translation="MTAAPASGPPVIAGARLESVSYSALDGWQDDDHAAAFAAFRLSC
                     RAIVEAAPELRLAQPAPPALVAACREGLALDSQDAAAARQFFETRFQPWRVRADGGAS
                     LLTGYFEPEVAGAMTPGQDFKAPVLGRPPDLVTLQPGNFDEALPPGLTSARRTSAGLE
                     PFPDRSAILDGALAGQGLERLYLKDAVEVFIVQVQGSARVRLADGRQLRLTYAGRNGH
                     PYTSIGRALIAQGSIAPAEMSLERLTSWLRANPEAGDRIMRLNRSYVFFEVSEVDDPA
                     RGPIGGAGIALTAGRSLAVDRNLWPYGVPIFIGADLDTLAPPLDRQRRLMIAQDTGSA
                     IVGPARADFFWGSGNEAGRRAGWTRHPLDFVVLWTRS"
     gene            50401..50955
                     /locus_tag="PLQ11_00255"
     CDS             50401..50955
                     /locus_tag="PLQ11_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HRK23361.1"
                     /translation="MTGRHPPRRGYLTREDMQLWAHVTQTVKPFRGRSAFVLPADLVE
                     TAPVKVTPPVAEPAPPAPVRFLPPLQPLDRRLKQRLTRGRKDIDGVIDLHGMRQAEAH
                     GALIDFIHRAHREHRSLVLVITGKGSTEGMSLEGRGVLNRLVPHWLTDPAIRRQIIGF
                     EPAGRSHGGSGALYVRIRKSRPPA"
     gene            51008..52006
                     /locus_tag="PLQ11_00260"
     CDS             51008..52006
                     /locus_tag="PLQ11_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HRK23362.1"
                     /translation="MIKTRYDLLCIGNAIVDVIAHAEETFLRRFGVAKGSMTLIDAHL
                     AETLYAAMGPATIVSGGSAANTAVGAGSLGGRAAYIGKIRQDSVGDIFAHDIRASGVS
                     FDTQRAEDGPPTARSFILVTPDGERTMCTYLGACQNLTEADIDQATVKAASITYLEGY
                     LWDPPAAKAAFRKAAAIAHSVGRDVALTLSDSFCVDRYRDEFLDLIRSGTVDLLFANE
                     HELKSLYQTADFNTALSAVRAQARRAVVTRSAKGALAVAGSETAEVAACPIDNLVDTT
                     GAGDLFAAGFLFGSTAGYKPEACLRLGALAASHVIQTLGARPQVSLRQLAIDNGLI"
     gene            complement(52026..52997)
                     /gene="coaA"
                     /locus_tag="PLQ11_00265"
     CDS             complement(52026..52997)
                     /gene="coaA"
                     /locus_tag="PLQ11_00265"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HRK23363.1"
                     /translation="MDARVSPLGSAVDLSPYRVFNREVWANLRADTPLTLTSSDLKKL
                     QSLNDPVSLDDVIEIYLPLSRLLSLYVAATQGLYKATQRFLMAEEGGKVPYIIAVAGS
                     VSAGKSTTARLLKALLSRWANTPKVELITTDGFLLPNAELQRLGLMERKGFPESYDAG
                     AILKFLARVKAGERNVEAPVYSHLVYDVLPDEVAVIDRPDILIFEGINVLQGPRKVKD
                     GKPIPFISDFFDFTVYIDADESDLHRWYVQRFMKLRETAFRDPKSFFRRYAELSEDEA
                     FRTANGLWERINLVNLRENIVPTRPRASLIMRKGADHRIEEVALRKL"
     gene            complement(53021..53338)
                     /locus_tag="PLQ11_00270"
     CDS             complement(53021..53338)
                     /locus_tag="PLQ11_00270"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HRK23364.1"
                     /translation="MPFTLSDLETIIARRASASPEESWTARLNAAGPARIAKKFGEEA
                     IEAVIAGAQGDRGALISESADVLYHLMVLLHSRGISAKDVLEELERRTAQSGLAEKAG
                     RSR"
     gene            complement(53372..54148)
                     /gene="hisF"
                     /locus_tag="PLQ11_00275"
     CDS             complement(53372..54148)
                     /gene="hisF"
                     /locus_tag="PLQ11_00275"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007009109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HRK23365.1"
                     /translation="MTLKVRVIPCLDVRDGRVVKGVNFVDLADVGDPVEAARAYDAAG
                     ADELCFLDITASHERRGTLLDIVQRTAENCFMPLTVGGGVRTAEDVRALMLAGADKVS
                     INTAAVRDPTIVARASERFGAQAIVVAIDAKQVSGPGETPRWEIFTHGGRTPTGIDAV
                     AFATLVAGYGAGEILLTSMDRDGTKNGFDTVLTRTIADAVTIPVIASGGVGTLDHLVD
                     GVRQGGASAVLAASIFHYGTFTIRQAKSHMAAAGLPMRMD"
     gene            complement(54145..54891)
                     /gene="hisA"
                     /locus_tag="PLQ11_00280"
     CDS             complement(54145..54891)
                     /gene="hisA"
                     /locus_tag="PLQ11_00280"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HRK23366.1"
                     /translation="MGAVILYPAIDLKNGQCVRLRQGDMDLATVFNDDPGAQARNFEA
                     QGFEALHIVDLDGAFAGRPMNAAAVDAVLAAIRMPVQLGGGIRDLKTVAAWLDKGVAR
                     VIIGTAAVRDPGFVRAAAVAFPGQIAVGIDARDGFVAVEGWAETSSLSAVDLGRRFED
                     AGVAAIIYTDIARDGMLSGVNWEATIALAEAVAIPVIASGGLASMDDIRRLVAPESAQ
                     LAGAITGRALYDGRLDPTEALRLVKAARAA"
     gene            complement(54879..55529)
                     /gene="hisH"
                     /locus_tag="PLQ11_00285"
     CDS             complement(54879..55529)
                     /gene="hisH"
                     /locus_tag="PLQ11_00285"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HRK23367.1"
                     /translation="MKVALIDYGAGNLHSAHRGLERALADIGRTGEVIVTADPDVVAS
                     ADRIVLPGDGAFRDCRGQLAAVSGLDQALDHAVRGKGRPFFGICVGMQLLADIGEEHG
                     ETAGLGWLGGHVKHLEPDGAGFKVPHMGWNTLRPLRKHPLIDGVRLGPDGLHAYFLHT
                     YHLVAADSSDMLAVADYGGQVTAMVARGPIAGSQFHPEKSQTLGRAIFANFLMWAP"
     gene            complement(55526..56005)
                     /locus_tag="PLQ11_00290"
     CDS             complement(55526..56005)
                     /locus_tag="PLQ11_00290"
                     /inference="COORDINATES: protein motif:HMM:NF022400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HRK23368.1"
                     /translation="MQSFTVHLADTAASENALEGAEFVSERFSMPALVFGAFWLAFNR
                     LWLALAGVLVVLALLAGIGHALSVPGPVQSAMSLLVHALIGLEAGALRRWTLERNGKP
                     TVAVVAARTLEEAEVRFFSNYRPMQAAPSAAMPSPTMPYPAAGSSVLGIFPAPGGRL"
     gene            complement(56005..56598)
                     /gene="hisB"
                     /locus_tag="PLQ11_00295"
     CDS             complement(56005..56598)
                     /gene="hisB"
                     /locus_tag="PLQ11_00295"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015822909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HRK23369.1"
                     /translation="MRKASLRRTTGETDIDVAINLDGSGRSEITTGVGFLDHMLTLLG
                     RHALFDLTVSAQGDTHIDDHHTVEDIGITLGKAFAQALGDKKGITRYADVHMPMDETL
                     TRVALDISGRPFLVFRTEFNAPKIGSFDCELVREWFQAFAINAGVTLHVETLYGSNNH
                     HIAESCFKGLARALRAAVELDPRARDLVPSTKGTLSV"
     gene            57048..57599
                     /gene="hslV"
                     /locus_tag="PLQ11_00300"
     CDS             57048..57599
                     /gene="hslV"
                     /locus_tag="PLQ11_00300"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HRK23370.1"
                     /translation="MQAHDHGQPGWHATTILMVRKNGETVIGGDGQVSLGATIIKGNA
                     RKVRRLGKGQVIGGFAGATADAFTLFERLEAKLEQYPGQLTRACVELAKDWRTDRYLR
                     RLEAMMLVADREAGLVLSGTGDVLEPEGGVMGIGSGGMYALAAARALMATDHDAETIV
                     RRAMAIAADICVYTNRELVVEKL"
     gene            57611..>58023
                     /locus_tag="PLQ11_00305"
     CDS             57611..>58023
                     /locus_tag="PLQ11_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRK23371.1"
                     /translation="MTDFSPREIVSELDRHIIGQSPAKRAVAIALRNRWRRQQLSGPM
                     REEVLPKNILMIGPTGCGKTEIARRLARLAGAPFLKVEATKFTEVGYVGRDVEQIIRD
                     LVEVAISLVREARRKDVRAKAELAAEERVIDALVGA"
BASE COUNT        10788 a        18641 c        17393 g        11201 t
ORIGIN      
        1 atctacatcg cagcagccgt catcaattca cgatgaatcc ccacgcgcct taggcggtca
       61 ggccgatctg caccccactt gatctgggcc gagtcctggt taacctgtct gtcatcagac
      121 aacagggtct tcgggaggca ttcatgatca agctgatgcg gctcgctctt gcggtatcca
      181 tctccggtct cgcaccattg gcttcggccc agagcccgtg gcccgacaag ccggtcacgg
      241 tgatcgtgcc ctatgctgcc ggcggtaaca ccgatgttgt cggccgcatc ttcaccgaac
      301 ggctgtccca aaggcttggc aagcccttca ttgtcgagaa caaatcaggt gccggcggca
      361 cgatcggcat cgccgcgatg agccgcgcgc ccgctgatgg ctatacgctc ggcgttgtca
      421 cggcgggaaa tcttttcatc ctgccgcata tctacaagga caagctcggc tacgacacct
      481 tcaaggacct gaaaccgctg gcaatggtgg caacgcaacc gaacttcttc gtcgtccatc
      541 cttcgatccc ggcaacgacg ccgaaggaat tcgttgatca cctgaaagcc aatccggaca
      601 aatattccta cggctcgtcc ggcatcggca cgtcgcagca tctgtgcatg gaattgctgg
      661 cgcagaaaac cggcatcaag gtcacccatg tgccctatcg cgcttccaac cagatcatgc
      721 aggatctgat tggcggcacc attcacatgt cctgcgatca gatttcgacc gcactggcac
      781 aggtcaggaa cggccgcgcc aagggcatcg ccgtgtcctc gcccgaagcc tacggccttg
      841 cgccggagta tccggcgatg gcagcgaccg tgccgggtat cgacgtgacc tgggcggcga
      901 tcttcatcgc cccggccaac ctgcccaaac ccatcgccga ccggctcacc agcgaactcg
      961 gcgcgattgc caaagacccc gatgtcatcg aaaagttgaa aggtttgacg gtgaccccgg
     1021 tctcggtggt gggcgatgcg ctggaaaaac gcatcaaggc cgatttcgag acctggaagc
     1081 cgattgtcga gagcgccaaa attccaccgc cgcaatagct cggttaacct ccgttaacca
     1141 tgtcgtccta gtgtagtgcg catgaaacgg aacggtgcct tcatgcgacg gatgtgtctt
     1201 gccttgccgt tgatcctggc tgcgctgcca gcgcgggcgg attttgcctc ctgtctggcc
     1261 gggttgcgtg aggctgcggc ggccagtggc atttcggcgc ggaccgtatc ggcggcgctg
     1321 tcgaacattt cgcccgacat gaagatcatc gaggcgatga acaaccagcc ggagttcaag
     1381 acgccgatct gggattatct cggtacgctg gtggatgacg aaaagatcgc cgagggcaag
     1441 gcaatgctgc gccgccatgc cgcgaccctg gctgccgccg aggagcgcta tggcgtcgac
     1501 cgccatgcca ttgtcgcggt ctggggtgtc gagagcgact tcggcaaggc cgggggcaaa
     1561 tggccgctgc cgcaggcact ttccaccgcc gcctgtctcg cgccgcgacg caacgccttt
     1621 ttcaagggcg agctgatcgc cgcgctgaag atcgtcgatc gcggcgatat tccgcttgaa
     1681 agcctggtcg gttcctgggc tggcgccttc ggacatacac agttcattcc caccacatac
     1741 ttgcgcctgg ctgttgatgg cgatggtgat ggtcgccgca atctcgtcag ttccattccc
     1801 gatgccctgc attccaccgc caatttcctg gccaagtcgg gatggcagag tggcgccggc
     1861 tggggttttg aggtgcgcgt tccggccggg tatggcggcg cgtcggggcg caatcccaag
     1921 cagccaattg cagcctgggc tgggcgcggc atcacccgca ttgacggcag cgccctgtcg
     1981 ggcggcggct ccgccggatt gctgatgccc gccggccgca atggcccggc cttcctggtg
     2041 ttccgcaatt atgacgcggc ctatgcctac aatggcgccg attcctacgc cctggcaatt
     2101 tcgctgctgt ctgatcggct gcgtggcaag ggcggcattc aggccccctg gcccaccgat
     2161 gatctgccgc tgagccgcga ggaacggcgg gaactccagc gcctgctgat cgcccgcggc
     2221 ttcgatgtcg gcgaaccgga cggcgctgtc ggcgccaaga cgcgcgcggc gatcaaatcg
     2281 gtcgaggccg agatcggtat gccccagacc gggcgacccg gatcgaaggt gttgcgggcg
     2341 ctgcgcgggc gataaacatt cccccaccct taataccaag tcgcaaggcg aggaactcgc
     2401 cgcgacttgg ttcgtttcaa aaatttctca cttttatcaa caagatggga aatttttgag
     2461 ttgcaccaaa gagtcatttt caatgacttt tggtataaaa gggcttggcc ttttgtccgc
     2521 atcgctttag gaacgcgcca gctttccctc cgaacgatgg cggatcctga tggcttccta
     2581 caatctcctg ctgcttcccg gtgacggcat cggccccgag gttatggtcg aggtgaagaa
     2641 ggtcctttcc ttctttaaaa aggccggtat cgcccggttt gaaaccgaga cggatctggt
     2701 cggcggatcg gcctatgacg cccacaaggt ggcgatcacc gatcaggcaa tgaaggccgc
     2761 acaacaggcc gatgcggtgc tctttggtgc ggttggcggc ccgaaatggg cgaacgtgcc
     2821 ctacgaggcc cggcctgagg cgggtctgct gcgcctgcgc aaggatctcg gcctgttcgc
     2881 aaatctcagg cccgccgtct gctatccggc gctcgccgat gcctcctcgc tcaagcgcga
     2941 tgtcgtcgag gggcttgaca tcctcatcgt gcgtgagctg accggcggcg tctatttcgg
     3001 cgagccaaag gaaatcgtca ccctcgagaa cggccagaaa cgcgcggtcg atacccaggt
     3061 ctacaccacc ggcgagatcg agcgcatcgc ccgcgtcgct tttgaactgg cgcgcacccg
     3121 ccgcaacaag gtctcctcgg ccgaaaagca caatgtcatg cgctccggcg ttctgtggaa
     3181 ggacgtcgtc accggcctgc acaaggccga ctatcaggat gtcgagcttg aacacgtcct
     3241 ggccgacaat tgcgccatgc agctggtgcg ctggcccaag caatatgacg tgctggtcac
     3301 cgacaatctg ttcggcgacg tgctctccga catcgccgcc atgctcaccg gctcgctggg
     3361 catgctgccg tccgcttcgc tcggtgcgcc tgtttccaga accggcaagc gcaagtcgct
     3421 ctatgagcct gtgcatgggt cagccccgga catcgccggc aagggcctgg ccaatccgat
     3481 cgcgatgatc ggctcgttcg gcatggcgct gcgctattcg ctggggctgg gcgaggccgc
     3541 cgatctcgtc gaaaaggcca ttgccgatgt gctcgcctcc ggcctgcgca cccgcgatat
     3601 cgctgcccag ggccagaaca ccatcggcac cggcgagatg ggcgacgcca tcgtcaaggc
     3661 cctgcagcgg ctggcctgat cggacttctt ggcttacgaa atagcaaagg gcgcctgcgg
     3721 cgcccttttt tctgtccgga acccaagtaa gcaggcgctc agcgcaccgc gttggcgccg
     3781 agaaactcga acgcaaatcc ggagccggac aggccgcgcg tcggcaggtt gctttcgcta
     3841 cggctggaaa tcccgtgcat cggcgcaccg ccgaatgcct gggtattggt cacgtaatcg
     3901 cgtccctggc cagcgcgcgc gacattgccg gcatccagcc aagagcgcgg tttgacgtcg
     3961 atgaccagcc cgcgatcacg ggcggccagc ggtgcgctga tcaggcttgt tgccacaagg
     4021 gcggcggaga ggagggcgat acgcatggag agaacctttc gacacctggg aggtgattca
     4081 cgctagccca atatggtgaa gaactggtgg atgataaagc gtgttttttg cctatcgcag
     4141 gcctgccatc gacgaaaacc cggctttcag gaccggcgca tcatcgccat ccatccagtt
     4201 tgaagcaatc gtgaccagcc gcgcagcacc gcagggctga cgggcccgtt ccacttcggc
     4261 gaaatacgat ttcagcctgg tatcgttgcc ggcgcgcaca agatcgatcc ggtcaatgag
     4321 acaggcaagt tccgcccggt ccaccgggcg atcggccgtg ccgcagaacc agccatgcag
     4381 atccagcacc gcctccgacg tgctgtgacg gaaggccaga cagccacgcg ccgcgcgacc
     4441 atcgctgctc aaggcgattt ctgccgtctc aagagcgccg aatttggtct tgagcccgat
     4501 cgtcgttccc agtcgctcga tgctggcgcc ggtcagcgca gcacggcgcg acaggtcgat
     4561 gaacagggtt tcgcgctctg gtcgaagcct gccatagctt tcgacaaaca gcgtgccgct
     4621 catcctggcg cgcgaccggc cggtcgaatc ggcttgtgcg gtccaggcca gaatgtcctg
     4681 acgcggacca tcgcggtggg tgcgggcgcg ataggcaagc gttgagcggt cgagttcggg
     4741 agcctccaga tgatagctgg cgatcggcga tgccaccgct ttccaggcgt ccggacgagg
     4801 ccgcgcctcc tcaacctcgg caggcttcat cgcaaccgcc tgggccggcg acggcccgtc
     4861 cttttcgtcc gaccacagca tggcggcgct gacggcaatc aacgtcaatc cgatgagttt
     4921 cacggcgcgc gggacacgcc agagcaaccg cgataccact ttgacgacaa gccagaccag
     4981 ggcgccgcta tccttgagtc tggtgaacag gcgagagcga tcgaggcacc tgtgagcgat
     5041 cgcctgcagg gcggatgaaa tgatcttggc cgaccgattc agcttggcag aagccagatc
     5101 cgacgccgca gcgccaaggg ctccggcacg ctcccagagg ggtgagagcc gtgtagccgg
     5161 cgctgcggtc gcgtcctggg ctttggccag acggtcaccg gccgcttggc gcgccgaact
     5221 gtagacgcgc cgcagccgcg cttcgaggct ctggtcctcg tggaccacaa accccgagat
     5281 ttcagaccgg attccggtac gcccttccgt tacgaaactc atcccgccct caaaccccgc
     5341 ttggcggcac caactccgcc caggctggca aaccccacat tgccgttcga acaaagttgg
     5401 cgtaaaagcg cggcttcccg gttaccggcc gggaaaaagt tatccggaga gtttacgatg
     5461 ggttacaagg tcgcaatcgc cggcgccacg ggcaatgtgg gccgggaaat gttgaacatc
     5521 ctttctgaac gggcattccc ggtcagtgaa gttgtcgcgc tcgcctccca gcgcagcgtt
     5581 ggcaccgaag tttcgttcgg cgacaagacc ctgaaagtca aagctctcga gcattatgat
     5641 ttttccgaca ccgatatctg cctgatgtcg gccgggggtg agatctccaa gcaatggtcg
     5701 ccaaagatcg gcgcccaggg ctgcgtggtc atcgataatt cgtccgcctg gcgctatgat
     5761 gccgatgtgc cgctgatcgt tccggaagtg aacgccgacg ccgtgcgcgg ctttgccaag
     5821 aagaacatca tcgccaatcc gaactgctcg accgcccagc tcgtcgttgc gctcaagccg
     5881 ctgcatgagc gtttcggcat caagcgcgtg gtggtttcga cctaccagtc ggtatccggt
     5941 gccggcaagg acggcatgga cgaactcttc gaacagacca aggcgatcta ttcggccgcc
     6001 gaaccaaaga cgaagaagtt ccccaagcgc atcgccttca acctcatccc gcatatcgac
     6061 gtcttcatgg aggacggctt taccaaggaa gagtggaaga tgacggccga gaccaagaag
     6121 attctcgacc cgaagatcaa gctgaccgca acctgcgtgc gtgtgccggt gttcatcggc
     6181 cattccgagg ccgtcaacat cgaatgccag aagccgatca ctgccgacga ggcgcgcgaa
     6241 gtgctgcgca cggcgccggg cgtgctggtg atcgacaagc gcgagccggg tggctacatc
     6301 accccgcacg aggcggcggg cgaggacgcc acctatatct cgcgcatccg cgaggatgcg
     6361 acggtggaaa acggcctggc cttctggtgc gtgtccgaca acctcaggaa gggtgccgcg
     6421 ctcaacgccg tgcagatcgc cgaagtgctg gtcaatcgca agctgctcaa gcctcgctcc
     6481 aaggctgcct gatcggtcat ctgtctgatc ggaacctggc cgccgcccgc aagggcggcg
     6541 gttttgtttg cggcgtatcg aatcgttcat cgttaacgac ccattattag actttcggct
     6601 aggatcagag gcagttcacg ccgggctgcc atgcccgctg tcctgacgag gccgtcgatg
     6661 agccacgagt ctctgccttc tcctgcccgc tcacgggtcg aacgcgctat tttgcgcctt
     6721 cagggcggcg gccttgccgg ggcaattctg gcatttctgc ttatcaagct ggccgggctg
     6781 ggaatcaccc tgctgtggcc gctgaaatcg gcaaaattcg ctccgcttgt gctgctctgt
     6841 tgcgccgaag tctcctggtt gttcggcggc accgtgggct tgctggtggt tctgcaatgg
     6901 ctggtgcacg aactggcgca tctgcatgtc tcccgccgtt tcggtcacat gccgggattg
     6961 cgcagtctga ccccgtttgt tggcgtctat cttggcacaa ccgctgcgca atggccgtcc
     7021 cgcgaggcag aggccactgt ggcgattgcc ggcgtgctgg ccggcctgtg cctggccttt
     7081 gctctgcatg tggcaggcag catgagcggc gacgaggcca tggtgctggc ggcccgcctc
     7141 ggcttcatga tgacgctgat tcacttgctg ccgttccggc ccttcgatgg tggccgcata
     7201 tttccggggc ttttctggtc gatccgctcc accaccgatc cggcagaacc catgcggatg
     7261 ggctccacca cgatacggct ctgtggctgg ctggtcggtc tgatcccggc gccagtgcgc
     7321 aggcgcctcg ctgtcaccgg atcggtactg ggagatggac tggtggccgc gctggcctgg
     7381 gccacggtca tcgtaaccct gtccggcctg gctctgccgg ccggcgcctt catggcaatc
     7441 atgctggcgg cgatcatagg ccttgtcgcc ttgtttgccg ctgtcctctc cattgacccg
     7501 cgccatggcc cggcggcgag ccggcgactg ttgccacgca acggccaggt aacggttgaa
     7561 acgtctgcca gacccttgcc ctggacggac cgcagcgccc cgcgcgccgc cttttgcatc
     7621 tacgcggcag cgctgcttgc ggcgatcgcc gggtcctttt gacccggctt gactacagcg
     7681 tttccagccg tcccgtggtc acatcaaaga tgcagccgta aatctccatt tttccgcggt
     7741 tcaccagcgt gctgatgcac ggaaaggtca acagattctc gatcgagttg cggatcgagg
     7801 tccgtgccag ccgttcggca taatcatgat cgctttcatc gtcctgcgcc ggcagcgatt
     7861 cggcggccgg ggcgatcagt tcgatccact tgccgatgaa atcgccggga gacagcacat
     7921 cggattgctg cgggcgccgc agccgcttga cgaaggcatg gacgccaccg caccggccat
     7981 ggcccatcac cacgatattg cggatcttca gtgcctgaac agcaaattcc agcgccgccg
     8041 agacgccatg caggccggcc atcggctcaa atggtggcac caggttggcc acattgcgta
     8101 cgacgaacag ctctcccgga ttggcatcga agataacctc gggagaaacg cgcgaatcgc
     8161 aacaggcgat gatcatcgtc tcgggcttct ggccatctcg cgccatctcg acaaagcgtt
     8221 ggtgttcgcg cggtaaccga ttggccttga acgcctcata gccttcaacc agccttggcg
     8281 gcaggccgtg ccgcccaccc tttggcaaat cagcattggc tgccatcgca tttctcctgt
     8341 cccggcctac atgggtagcg ccgcagcttg cgacaaggag gcaaatcgcg gcatacggtc
     8401 aaaatcacca taggtgcagc cggagaccct ccatgtcttt ccgcccacgc cgcagcgcgc
     8461 tctatgttcc ggccaacaac gtccgggcca tcgaaaaggc cattacgctg ccggcggatg
     8521 tcatcattct tgatttggag gatgcggttc cgccccaggc caagaacgaa gcgcggcgga
     8581 atgccgcctc ggccattcga tcccaggcct ttcgtgacaa ggagcttgtt ctgcggatca
     8641 atgggctcga cagtgacgag gccgccgaag atctggtggt gggggccggt tctgacgccg
     8701 ttctggtgcc caaggtccag gatgccaatg acatccaccg ggcagaggcg ggcctgaacg
     8761 agatcgccgg caaggaccgg ccagcccttt gggcaatgat cgagacgccg aaggcgattc
     8821 tcaacatcgc cgccatcgct gcggttgcgg caagagacga tgcgcgcctt gcctgtttcg
     8881 tgctgggcac caacgacctt gcaaaggaca ccagggtcaa tctgaccggc agccgtctgg
     8941 ccatgctgtc ctggatcagc cagacggttc tggccgcccg cgcctacgat cttgcggtac
     9001 tggacggggt ctataacaac ctcaaggacc cggatggtta tctggccgaa tgtcggcagg
     9061 gtcgcgggtt cggttttgac ggcaagacgg tgatccatcc cagtcagatc gccgacgcca
     9121 atcacatctt tggaccgacc gacagcgaga tcgccgaagc gcgtgcgatc gtcgaggcgt
     9181 ttgcccgacc ggagaatgcc ggcaagggcg tgctgacggt cggagggcgc atggccgaac
     9241 tgctgcacag ggaccaggcc gaacgaacac tggccattgc ccgcgccacg ggcctggcct
     9301 gacccgcccg ccgatatgac ggatcgcctt acttgctgct ggccttgtag cgctcgattc
     9361 cgtcgacgat cagcttgcgt gcctcatccg catcgcccca ccccatgagc ttgacccatt
     9421 tgccgggctc aagatccttg taatgggtga agaaatgctc gatctgcttc catgtgatgg
     9481 tcggcaggtc ggtatagtta tggacatgct cataccgctt ggtcagtttg ggcgagggca
     9541 ccgcgatgat cttctcgtcg ccgccaccat catcctgcat cttgagcacg ccgaccggtc
     9601 gaacattgac cagagcgccc ggcacgatcg gccttgtatt ggcgatcagc acgtcgcacg
     9661 ggtcgccatc atcggacagg gtatggggaa tgaacccgta attgcctgga taatgcatgg
     9721 gcgtgtagag aaagcgatcg accaccaatg tgccggacgc cttgtcgagc tcatacttga
     9781 tcggctcgcc accgatcgga acctcgacga tcacattgac gtcaaacggc ggattcttgc
     9841 caatctcgat tgcatcgata cgcattgacc cctccagctc aatcaggttg atctttaagg
     9901 ggcccggcgc cgttttccaa tcgcgccttt ggtatcgcgc cggcaaattg tcgatcaatg
     9961 tgaaggaagt gttggcactg gagcattttc gagcgaagtg ggttccggtt cgcgtcaaga
    10021 aaatgcgaaa aacaaagaaa ttgggcactt tctgtgagtt ggctttcgtc gaaagcgcac
    10081 cagagcacgc cctcgttccg gtcgaatcgc cgaagcgaga gataacgtga ccgataacaa
    10141 ctattttggg catgcttttt gcgaaaagcc gttcacgctt gttcgcagca tgctctcgcg
    10201 cccgttgtct ccggcaggac ggactgctac agccctttgc gcctatggtc tttgcaatct
    10261 tccgccccga acgccgctac gcccggcgcc tggctcgtgc cgcccgctgg gaccggccga
    10321 ttgccggtcg ctggcagcga ttctgcgcct ggaccaacat gctgctgatc gatcatgggc
    10381 tgttccggct gatttatctc aatgcccatc cggtcaccga cgatctgtgg cgctcggccc
    10441 agccgtcgcc gcatcaggtc gccgactttg ccgcgcgggg catccgcacg attgtcaacc
    10501 tgcgaggagg gcgcgagttc ggctcctggc cactcgagcg tgaggcctgc gacagggccg
    10561 gaataaccct tgtcgacttc gtcgcacggt cacgcgaagc ccccgggatc gacaccgttc
    10621 tggccgccca gaagctgttt gacagcatcg aatatccggc actgattcat tgcaagtccg
    10681 gcgccgaccg tgccggcttc gtctcggcgc tctatctgat gttgaagcat ggtcagccgg
    10741 cttcgcttgc gcttgaacag ctttctgccg gttatggcca ttggcgctgg tcgaaaaccg
    10801 gaattctcga cgccttcatc gaacgctacc gcgaccaggg cgaggcccgg ggcctgtctc
    10861 tgatcgactg ggtgacaacc gaatacgacc ccgacgaact gacccgttca ttcaggccac
    10921 acgcgttctc ggcctttatc gtggacagga tcctcaggcg ggagtgacgc cctcgccgaa
    10981 ctggagccgg tagaaggccg cataggcgcc atcgctggcc atcaggctgt catgcgttcc
    11041 ggtctcgacg atgcggccct cgtccatcac cacgatgaga tcggcggccc gcaccgtcgc
    11101 cagacgatgg gcaacgacca gcgtggtgcg gccgcgcatc agatcgccca gcgctttctg
    11161 gacgagatcc tcggattggg catcaagcgc ggaagtgggt tcgtccagca ggagaatcgg
    11221 cgcgttgcgc aggaatgccc gggcaagcgc aacgcgctgt cgttcgccgc ctgacagccg
    11281 tcccccccga tcaccgacgc gcgtatcata gccatccggc aaagccgcga taaatccatg
    11341 ggcggctgcc gcgctggcgg cggcgatgat ctctgtttcg ctcgctctct gccgaccgaa
    11401 agcgatattg gcccggatgg tgtcgtcgaa aaggacgata tcctgcgata ctaccgccag
    11461 atgcgcgcgc aggcttttaa gcgacactga acggatatcc gttccgtcga tcgtgaccac
    11521 ccccgcagtc ggatcgaaca ggcgcggcac cagggccagc aggctggatt tgccggctcc
    11581 cgaacgaccg acgaatgccg ttgtctttcc cgccggcacg acaagggaga cgtcggaaag
    11641 ggcagagccg gaattggcgt aggagaacga caccccggca aaggcgacct cgccctgttt
    11701 cacaaccagt tccggcccat cgaccgcatc ttcgacctgt ggacgttcat cgatgaccgc
    11761 gaagatccgg gtcagtgcgg ccaccgcctc gtgcaggatc gcgttgagat tgccgagtgt
    11821 tcgcaggctc tgggcagcca gcagaacggc ggtcacatat ccggtgaagt ctccgatggt
    11881 cgattgcccc tgggcaatgc gccatccgat aaacaccagc accgccgcaa ccgtcacgcc
    11941 gccgccaacc tcgagcagcg gatcaagccg cgcccgctga ttggcagcct tgaccttgag
    12001 ccgacggaca gcatcgaagg catcggcggc cttggatttc aggtagggtt caagaccata
    12061 cgtcttggca atgcgcacac cgccgaggct ttcggccacc aggctcgcca tgatgccggt
    12121 ctgttcctgt gtcgacagcg ccgtccgccg caattttttc ccgatccgct cgatcggcgg
    12181 gacgatgatc ggcagaacca tcagtgcggc cagcgtgatc atccagtcga tccacaccat
    12241 gaccgcaaga accgcaacgg cggtggcgac atcgcgcgcc agcaccgtgg aaagccgtgt
    12301 caacgcctca cgcacatagg caaaatcggt ggtaaagcgt tgagtaagcg atgcaggagt
    12361 ctcgcggctc agctgcgcca catcggcgtc gattaggctg tgaaacagcg aggtctgaag
    12421 atcggcctca accttggaga ccacccggtt ggtcagaatg agctgggcga acattgcaaa
    12481 gccgcgcatg gcggtcacca tgatcaccag aacgggcgtc agctttagaa acgcctcatc
    12541 cttggcatca aaggcatcga aggctcgctt gatgatgtag ggatacatcg acgacatcgc
    12601 cgcgatcacc agcacggtca gccagataat caaaatcgtg ccgccatgcg gccttaccca
    12661 accctgccac aggcgtcgaa cggccggtat cagccgctgg cggtcgtgca gcagctttgc
    12721 cggtggatca ggcggggccg aagccgaagc gtcggtcatc cctctactgt ccagatcgaa
    12781 atcggtggga aaggaacagc aagcatctcg tcatcggtca ccgtaagggg caggcggtga
    12841 ctggagcggg cgaagggatt cgaaccctcg accccaacct tggcaaggtt gtgctctacc
    12901 cctgagctac acccgcttgc gccggaccaa gccggctgcg gcgctcgtat gcaacatttg
    12961 gcgtttgcgt gcaagcccgt tatcaagcga tccgcgcttt tcattgttgc tccggatgac
    13021 ggctgatgac ctcttgcccc gatattcctg tcgcccgccc gctgtttgac ctcatggacc
    13081 gtctcgccat cgcgcatcgg acgcttcagc atgtcgcagt ctataccgtg gccgaatcgc
    13141 gggagctgcg cggcaccatt cctggcgcgc actccaagaa cctgttcctc aaggacaaga
    13201 agggtcggct attcctggtt acggcgctgg aggatgcccc gctgaacctc aagaagctgc
    13261 atgaagccat tggcggccag gggcgcgtat cgttcggctc ggccgaacaa ttgcgcgcct
    13321 gtctcggggt cgagcccggt tcggtgacgc cctttgccgc gatgaacgac accgacggcc
    13381 gggttaccgt tgttctgcaa cgcaagctcg cggaagcgcc ggttctcaat ttccacccgt
    13441 tgaccaatac cgcgacgact acgatcacca atcaggatct cctgcagttc cttgcggcca
    13501 tcggccatcc cccgcatatc gtcgacctgc ctgaaggtga ggcgtcctca ccagaggcgt
    13561 gatgtgtcat tgcctttcct gtccaccgac cccatgtagg atggggaagg ttcttttctg
    13621 atacacttgc cgaggttcac atgctgggcg cccaacccga aggcctgatc aaggatacga
    13681 cgacgcaagg tttccgcgcc gacgtcatcg ccgaatcggc gcgccagccg gtgctggtcg
    13741 atttctgggc gccatggtgt ggtccttgca aacaactcac tccggtcatc gaaaaggcgg
    13801 tgcgtgccgc gcgcggcaag gtgcgcctcg tcaagatgaa catcgacgac catccggagg
    13861 ttgccggcca gctcggcatt caatcgattc cggcagtcat cgccttttcc aatggcaagc
    13921 ccgtggatgg attcatgggc gccctgccgg agagcaaggt gacggctttc atcgaacgat
    13981 tggtcggtcc atccgccgat gatccggcaa ccctcatcaa gcttgccgag gagcgcaagg
    14041 cgggcgggga catcggcggc gctgcggaag cctacgcggt tgttctggcc aaggatccgc
    14101 accacggtgc ggcgctcgct ggccttgccg ggctgcagat ggacaccgac aacctcgaac
    14161 aggctcgccg gatcctcgat tccgttcctg aagccaagcg gacggagccg gccatcgtgg
    14221 ctgcaatcgc ccgtctggag ctgatggaac gggcggccag ccttggcgac cttgccgatc
    14281 ttgagcgccg cattgccagc aatcccgccg accatcaggc acgtttcgac cttgcggtcg
    14341 ccctcaatgc gaagggtttc cgcgccgagg ctgccgatca cctgcttgac atcatcaaga
    14401 tggatcgaag ctggaatgat gacggcgcac gcaagcagct cttgcaattg ttcgaagcct
    14461 gggggccgat ggacgaggtg acgcttggag cgcgccgccg cctttcggcc tatctttttc
    14521 ggtaggcccc ataacgccat ccggattgag gagcagaggc aatgccgtcc aacgtccagt
    14581 atcaggggcc agcgcaactg ccggaggtca tcccgatctt tccgctcgcc ggcgcattgc
    14641 tgctgccgcg tggccagatg ccgctcaata tcttcgagcc gcgctatctg gccatggtcg
    14701 atgcggccct cgccggtgac cgtttgatcg gcatgatcca gccgatcaac gaaaaggccg
    14761 gcgaggcacc gccagatctg atggctgtcg gctgcgttgg ccggctgacc gaatttgccg
    14821 agacgggcga tgggcgctac ctgatcacct tgacgggcat tgctcgcttc cgcctcattg
    14881 aggaggtcgt taccgacgcc cccttccgca aggcccaagt ttccaccgcg ccgttccggc
    14941 ccgacttcat tgcccgcctg ggtgaggatg ctgtggatcg tgaagcagtc ttgatcgcct
    15001 tgcgcgcgtt ctccgatgcc aaggcggtcg agatagactg ggagagtgtc ggccaggcct
    15061 ccaacgaggc gcttgtcaat gccctgtcga tgatgtgccc ctacgggctc aaggaaaagc
    15121 aggcgctatt ggaagcgcca gacctgaaaa cccgcgccga gctgcttatc gccctgaccg
    15181 aaatcgatct gtcggggccg ggcggggatg gcgacataac cgtccagtga gtaagatgac
    15241 cggttccaac gcccccgagc gctatgccac gcgcagcatc gaccccaagt tccttgaact
    15301 cctggtctgc ccgctgacca agaccgcgct cgactatgat gccgtgcgcc aggaactggt
    15361 gagccatgca gccaagctgg cattcccgat ccgcgacggt atcccgatca tgctggtcga
    15421 cgaggcccgc cgccttgcgg attgacgggc cgatcagatc ggcagtccgc gaaaggcccg
    15481 cggcgctcgg gaactgtcac cggccgcttc ctgcatgaag cgcgcgccca gtcccggcag
    15541 ccgatcgacg agcgaaagcc ccaggtcacg gatcgcccgc agcggaccga aatcgttgga
    15601 aaacagccgg ttgagtgttt cggtggccgc agccatcgcc atgacatcgg gccttcgggc
    15661 cgcctcgtag ggcccgagcg ccgtctccga tccgatatcc agccccagcc gggccgcgtc
    15721 gatgatcgtt tcggccaacg ccgccgcccc gcgcagcccg tagttgaggc cctgtccggc
    15781 aatcggatgg aagccatgcg cggcatcgcc cagcaacgcc agtcgtggcc cgacaaaccg
    15841 ccgggcaata ccgatcgaca tcggaaagga cgccaatccc tcaacgcgcg tgaccgttcc
    15901 gaaccgcccg gcggcgcgcc ggtcaatctc ggtgacgaga tcgtcggaat cgcatctcag
    15961 caaccgttcg accgtctccg gccgctcgtt ccagacgatc gagcagcgcc gacccttgag
    16021 cggcagcaag gcaaacggtc ccgccggcag gaaatgctgt accgcgaccc cttcatggtc
    16081 gagtgtatgg tggatggtgc cgacaatggc cgtctgtgga tagcgccagc catgataggc
    16141 gatgccggcc cgttcgcgca gccgcgaacc gcgcccgtcg gcgatcacca ggagtctggc
    16201 acgcagccgc cgtccgccga cgatcgcggc gatgcgcccg cccgtctggt caaatccact
    16261 gaccggttca gaaaaaagcg acagtccggc cgaccgggcc ctggccatca gcgctgcaag
    16321 gatggttccg ttcgccagca tcatcgccgg tggtgcctcg tcctgcgtgg cggccccgaa
    16381 ggtcagcaac ggcattcgca ccaggtcttc gagccggctg tcggtaattt ccatgcgaag
    16441 gatgggttgc gcctcgtccc gcatcgcctg ccaggcgccg atggagtcga aaaaccggat
    16501 ggcgccggcg acaattgcat aggcgcgcga atcgccacgc cggtcccgcg ccagttccgg
    16561 gtcgcagaca gctaccttga gcgccgaacc agcggccaca ttgagtgcca gcgccagcgc
    16621 cagaccggcg gctccgcccc cggcgatcac cacatcgacg tcatcgatca cagccatgcc
    16681 tcaccttgac gcttcggcca ttcccttgcc aagtggcgct cccgtcccgc ttgaaccggc
    16741 cccgtcatgt ccgtcatttc cgatcttctg accatactcg atcttgagcc gctcgagcag
    16801 actctttttc gtggccgtgt gccgcaggtc ggctggcagc gggttttcgg cggcctcgtc
    16861 atcagccagg cgctggtcgc cgcccatcgc accgtcgacg gacgcgaacc gcattcgctg
    16921 catggctatt tcctgctcgg cggcgatccc agcgtgccga tcatctacga tgtcgaccgg
    16981 atccgcgacg gacgcagttt caccacgcgc cgtgtggtgg ccatccagca tggccgaccg
    17041 atttttgcca tgtcctgttc attccatgag ccggaagacg ggttcgatca tcaggcgacg
    17101 atgcccgatg tgccgatgcc ggaagatctg ccatccatcg aaacggtgaa gaaatcggtc
    17161 ctgcccaatc tgccgcatcc cgtacgcgcc tatttcgagc gcgagcggcc gatcgaactc
    17221 aggccggtcg atttcgagcg ttacaccgcc agcggttcag cccagctcgg aacccagatc
    17281 tggatccggg cagccggtcg cctgcccgat gatcccggca tccaccaatc cgtcctggcc
    17341 tatgcgtcag acatgacgct gctcgattgc tctctggttc cgcacaacac ctcggtcttt
    17401 gatcctggca ttgcccctgc cagtctcgac catgcgatgt ggttccatcg cccgtttcgt
    17461 gccgacgaat ggctgctcta cagccaggag agccccaacg cctgcgctgg ccgcggcttt
    17521 tcacgcgggc tggtgttccg gcgggatggc acgctggttg cctcggtggc gcaggagggg
    17581 ctgatccgcc gcaagcgtcc gtagaccatc gaggcgccgc cgtgatgaga ggcagcgtgc
    17641 cgttctttta ggcagggcag actatccttt cagcaacatt tcgcccggag gggcagaatc
    17701 agcctgatat tgtgcgagag cgagccccaa taactggcat gaagcttgtt tcggcggcgg
    17761 tgggcttggc cgcgcggcac ggcctgccgt ttgccccttc tgtcacgagg ccccgccatg
    17821 aaccctcgcc tgttttctgc ttcagcgctg ctgatgagcg ccgctgttgt cgaccccgcc
    17881 cttgcccagt cgacatccaa gatcgatgct gccgataccg gattcatgat tgtctgcacg
    17941 gcgcttgtct tgatgatgac gctgccgggt ctggccctgt tctatgccgg aatggtgcgg
    18001 cgcaagacag tgctggcgac catggcccaa tcggcggcct cgatcgccat tgtctcgctg
    18061 ttgtggatcg cggtcggcta ttctctgacc ttcagcggag acggaccgat tttcggcgat
    18121 ttgtcgcggg cgtttctgct gggcatcaag ccggatacga ccaccgcgct ggcgccgacg
    18181 atccctgaac tcctgttcat gctctaccag atgaccttcg cgattatcac ctgcgcgctg
    18241 atcggcggca gcgttgccga tcgcatgcgg ttctcggcct tcctgatctt ctcggtgctc
    18301 tggctgttcc tggtctatgt gccgagtgcc catatggtct ggggcaacgg ctggctggcc
    18361 aagctgggcg tgatcgattt tgccggcggc acggtggtcc atatcaatgc aggtatcgcc
    18421 ggtctggtag ccgcgctgat gatcggcccg cgtcacggct atggccgcga caatttcgcg
    18481 ccctatgatc tgtcgatggc tgtggtcggc accgggctgc tgtgggtcgg ctggttcggc
    18541 ttcaacggcg gctcggcgct cggcgccggt tcgcgtgccg tctatgcgat caccgccacc
    18601 catcttgccg cctgtgccgg cgctgtggtc tggactggcc tcgaatggat ccagcggggc
    18661 cgtccttcgg ttctgggcat catttccggt gccattgccg ggctcggcac ggtgacgccc
    18721 gcctccggct ttattctgcc ctggcatggg gttgtcatcg gtgtgatcgc cggcgccctg
    18781 tgctactggg cctgtatctg gctcaagcac aagctcgcct atgacgattc gctcgatgtt
    18841 ttcggcgtcc atggtgtcgg cggcctgttg ggaaccatgc tggccggtgt ctttgccaca
    18901 ggcagcctgt cggcgacgcc ggaattggcc gagggcttgc gcggtcttgt cgacggcaat
    18961 ccggggcagg tcgtcaccca ggcgatcgga gtggtcgtta ccacgctctg gtgtgccgcc
    19021 ggcacgttcg ttgccctgaa ggtgacggcg ttcatgaccc cgctgcgcgc cagccgcgat
    19081 gaagaggtcg agggcctgga tctggccctg catggcgaac gcctgcactg accaaattgc
    19141 caatgcctgc actctcgccc cggcggccat caccgccggg gccgcctttt tgcgctgcac
    19201 catcaacgca tgcatctgcc catcttttga gcaaggcggc gcccggatag ccaggacttc
    19261 agccgaatcg gccggaatcg ggcggcaaat cccgtctggc acgccgcttg tagctgacgc
    19321 ttcatccagc gcggattgtc cgatccgcga tccaaggcag aaggagcgtc gagcccatga
    19381 aaaccgtcac agccatcatc aagcccttcc ggctcgaaga ggtgcgcgat tcgctgaccg
    19441 cccttggtgt ccacggcatg accgtgaccg aggtgaaggg ttacggccgc cagaagggtc
    19501 ataccgaaat ctaccgtggc gccgaatacg cggtgtcttt cctgcccaag ctcaagcttg
    19561 aggtcggcgt ctccgatgaa ctagtcgggc cggtggttga ggccatcgcc gaagccgccc
    19621 gtaccggcca gatcggcgac gggaaaatct ttgttgcgcc cctcgatcag gtcgtccgca
    19681 tccgcacggg tgagcgcgac ggcgacgccc tctgaattcc tgtaagaagt ggagcttcca
    19741 tcatgtcctg caaatcgatt gttcttcgcc tcgccgcccc atgtctggtg gctggcctga
    19801 cgctcgctcc tgcctttgcc gatacgcccg ccccgcagcc caacaagggc gatacggcct
    19861 ggatgctgat cgcaaccgcc ctggttctga tgatgtctgt gccgggtctg gcgctgttct
    19921 atggcggcct tgtgcgcgcc aagaacatgc tgtcggttct cacccaggtc ttcgccatcg
    19981 tctgtcttgc cgggctgatg tgggtggcgg tcggctattc gctggccttt accaacggcg
    20041 gcgccctcaa tgattttgtt ggcggattcg gcaaggcgtt catggccggc atcacccctg
    20101 catccaatgc cgcaaccttc tccaacggcg tggtgattcc agagtacctt tacctcgcat
    20161 tccagatgac ctttgcgatg atcacgccgg ctctgatcgt cggcgctttt gccgagcgca
    20221 tgcgttttgg tgcggtgatg gctttcaccg ccctgtggtc gctgctggtc tactatccga
    20281 tcgcccacat ggtctggtac tgggcaggac ccgacgcgct cgccgatgcg gccaaggctg
    20341 ttgcggcagc cacggacgcc gtcgcaaaga ccgctgctca agccaaactt gacgaggccg
    20401 ttgccgatgc aggcatgatc ttcaagtggg gtgcactcga ctttgccggc ggcaccgtgg
    20461 tgcatatcaa cgccggtatc gccggccttg tcggctgcct gatggtcggc aagcgcatcg
    20521 gctatggccg cgagctgatg gcaccgcatt cgctggtgat gacgatgatc ggagcggcgc
    20581 tgctgtgggt cggctggttc ggcttcaacg ccggttcttc gctcgaagcc aacggcacgg
    20641 cggtccttgc cttcgtcaac acgatggtcg ccacctgcgc ggcggcgctg gcgtggctca
    20701 tcgtcgaatg gctgctcaaa ggcaagcctt cgctgctcgg catggtctcc ggtgccgtgg
    20761 ccggtctggt tgcggtgacc ccggcctcgg gctttgccgg gcccatgggt gccatcgtgc
    20821 ttggctttgt ctcgggggcc gcctgtttca tcttcgtcgc ctatgtgaag ggcgccctgg
    20881 gctatgacga ctcgctcgac gtcttcggca tccattgcat cggtggcatt ctcggcgcga
    20941 ttgctaccgg catactggcc agccccgacc tgggcggcgc cgggatcgtc gattacgtcg
    21001 ccaagccggg cgaagccgcg ccgggcgatt tcgtgatggc aacgcagctt atggcgcagc
    21061 tcaaggcggt ggcggtcacg ctgctctggt cgggcctggt ctcgatgatc ctgttcaagc
    21121 tgatcgatct ggcaattggc ctcagggctg aaagcgacga tgagcgcatg ggtctcgaca
    21181 ttgccgaaca tggcgagcgc gcctacagcg cctgatcgac aggcaacgcc atagcgatcg
    21241 gcggccacaa cggccgccga tcgcacatca ggcaaccatt tcttaagctg gatttaaccc
    21301 atcctgaacc atgatccgga ccagactgcc ctcagtatgg aatggatcat gcggacgacg
    21361 cgactgggtt cttcggcgac ggattacatc cccgaggcaa tccgcgacgg tatcatgcgg
    21421 cgcctggcag aactcgccgg gctcatcctg ctgctcgccg ccggcgccgg ggcgctggcg
    21481 ctggcaacct ggtcggtgcg cgatcccagc ctcagccatg ccgttgccgt tccggcacgc
    21541 aaccttctcg gttaccccgg cgcggtcatt gccgatctgc tcatgcagat tttcggcctg
    21601 gcagcaatcg ctctgctgtt ggtgatggcc atcatcggtt ggcggctgat ggcgcggcgg
    21661 caattgcaac gcccggcgct gcgattgatc gtggccatgg ttgcggtgat ctgcgccgcc
    21721 ggactggccg gaaccttgcc acgcacggac cgctggccct tgccgggtgg tcttggtggt
    21781 ctggttggcg atctcctgct ctatctgccg cgtctgctga ccggctcgct cgacatgctg
    21841 ctccgtctgc cggtaggctt tgcctgcgcc gtcggcctga tcatctcgat cgcctatgtc
    21901 ggccgccgct ccagcgagga ggaggcagag gaacaggcgc gcatcgccga ggaggacgtc
    21961 gaatccagcg ctgatgacga accgtctgta accatcatct cgctcggcgc tgtcatccat
    22021 gcattgttca gcgccaggag ctggattgcc cggcgctttg cccttcttat gggtcgccag
    22081 aggatgagca gcgaggcacc ggcccggcgc cggacgccgg catcccggag aaccgagccg
    22141 actttcgagc cgaccggacc ggttcaagcc ccggcaaagc gcacgcgcgc gctcccggag
    22201 ccggacttcg acagcctcga cgacactccg ttcgacgatc cccagccggt caaggccaag
    22261 gtcagtgctc cggtcggagc gctcaagccc ggtcgccggg ccgagcgcga ggcccagcct
    22321 tcaatgttcg gccaggatgg ctaccagcat ccgccgctga ccctgctgtc cgagcccaaa
    22381 cgggcacccg gtgtcatcat ctcaaaggat gcgcttgagc agaatgcccg cctgctggaa
    22441 ggcgtgcttg atgatttcgg cgtgcgcggc gaaatcatca acgtgcgccc cgggccggtg
    22501 gtcaccctct atgaactcga gccggcgccg ggcaccaaat cctcgcgcgt catcagcctg
    22561 gccgaggata tcgcccgttc gatgagtgcg gttgctgccc gcgtcgccgt ggtgcagggc
    22621 cgcaatgcaa tcggcatcga attgccgaac gccaagcgcg agacggtttt cctgcgtgaa
    22681 ttgctggcct gcgaggattt cgagaaatcg aagatgcggc tgccgctctg cctcggcaag
    22741 acgatcggcg gcgaaccggt gatcgtcgag ctcgcccgca tgccgcatct gctcatcgcc
    22801 ggcaccaccg gatccggcaa gtcggtcgcc atcaacacga tgatcctgtc gctgctctat
    22861 cggctcactc cggaccagtg ccggctgatc atggtcgatc cgaagatgct cgaactgtcg
    22921 atctacgacg gcattccgca tctgctcacc ccggtcgtca ccgatcccaa gaaggcggtc
    22981 gtcgccctca aatgggcggt gcgcgagatg gaggagcgct accgcaagat gtccaaggtc
    23041 ggcgtgcgca acatcgacgg cttcaatgcc cgcgtcgccg aggccaggga caagggcgag
    23101 gtgatccagc gcatggtcca gaccgggttc gaccgcgaga ccggcgagcc gatcttcgag
    23161 aacgaggtga tggatctgca gccgctgccg ttcatcgtcg tcatcgtcga tgagatggcc
    23221 gacctgatga tggtcgccgg caaggagatc gaggggctcg tccagcgtct tgcccagatg
    23281 gcccgcgccg ccggcatcca tgtcgtgctc gccacccagc gcccttcggt ggacgtcatc
    23341 accggcacga tcaaggccaa cttcccgacc cgcatttcct tccaggtcac ctcgaagatc
    23401 gacagccgga cgattctcgg cgagcagggc gccgaacagc tgctcggcca gggcgatatg
    23461 ctctacatgg ccggcggcgg ccgcatctcg cgcgtgcatg gcccgttcgt ctccgatgcc
    23521 gaggtcgagc atgtcgtcgc ccatctgaag gcgcagggcg cgccgcaata tcttgatgcg
    23581 atcaccgccg aagaagagag cgccgaagag agcgatgacg gggcgatatt cgacaagggc
    23641 aacttcggcg aggaaccgca ggatctctat gacaaggcag tgcaggtggt gctgcgcgac
    23701 aagaaatgtt cgacctccta catccagcgc cgtctgcaga tcggctacaa caaggctgcc
    23761 agcctcgtcg agcggatgga gaaggaaggc cttgtcggcc ccgccaacca cgccggcaag
    23821 cgtgaacttt tgctcgaggg cgccgaggca ggctgaggcc gggctggccg agttttgcaa
    23881 atccgctttg cagtttgacg cgaatccgcc acaaccccgc tctagtctcg ggtcgatgat
    23941 gaccgattcg gcgggagcta ccggtgaaga caacgtcatt cctggctgct ggttttgccc
    24001 tgttgctcgc cattggcgga gcgctggcgc agcttaacct gaatcctccc gccgcaacca
    24061 cgccgggcgc ccccaagggg cctgcaccgg cggcgcctgc accggcagct gccgccggcc
    24121 agaacgagac gcccagaccg acggttgagc gggtcaacgc ctatttcaat gccatcggcg
    24181 gcatgacggc tgattttgtc cagatcggcc cggacggccg gcgctgggaa ggcaagctgt
    24241 caattctccg gcccggtaag atgcggttcg aatacaagcc gccgagtccg cttgaaatca
    24301 tctccgacgg caccaccgtc gcggtgcggg atcgcaaggc caatacccag gatgaatatt
    24361 tcgtcggtca gacaccactg aaattcctgc tgcgtgaccg aatcgatgtc gcgcgggacg
    24421 ccaaggtgat ttcgctggcc cagcagggca gcgatgccgt gctggtgatc gaggaccgac
    24481 agactgtggg cggcacctcg cgcatccgtc tgaccttcaa caatgccgac atgatgctca
    24541 agcaatggat cgtcaccgat ccgcagggct atgacacgcg cgtccagctg gccaatatcg
    24601 acaccacccg gcggccgaat gcggccctgt ttgtcattcc gaccaaggtt tcgacaccga
    24661 actgaccggc gaacacgcgc ctgacccaac aattcggtgg atggcgcagt ctcatttggg
    24721 ctcaaggcgg cagcatgtct ttccggcagc ctatgcaccg gccataactc tgccatgaca
    24781 ctgacgattg ccacctggaa catcaattcc gttcgcctgc gcctcgatct tgtgctgcgt
    24841 tacctgtcgg aggcagcgcc cgatgtgctg tgcctgcagg aaaccaaatg ccccgatgcg
    24901 gaatttcccc ggtccgcctt cgtcaaggcg ggctatccgc atattctgca tcgcggcatg
    24961 aagggctaca acggcgtggc catcctgtcg cgcctgccgc ttgcggcagc ggagaacctg
    25021 accctgtgcg ggcgggagga cgcccggcac atcgccgccg aggttcggcc ggccgatggc
    25081 ccttccttgc ttgtccataa tttctatgtg cccgccggcg gtgatattcc cgaccggatt
    25141 gaaaacccga agtttggtca caagctggat ttcctggcag aaatgaccga ttgggctgag
    25201 cgcctgcgcg cccgtcacga tcgggctgtg ctggtcggtg atctcaacat tgctccgctc
    25261 gtccatgatg tctggagtca caaggcgttg ctcaaggtgg tcagccatac accgatcgaa
    25321 tgcgaggcgc tgctgcactt gcaggacagc ggcggctgga tcgatgcggt gcggtgccta
    25381 aatccggagc cggagaaggt ctacacctgg tggagctatc gctcgccgga ctggaacgct
    25441 gccgacaagg gcaggcgtct cgaccatatc tgggtcgggc cggctttgaa gccggctatt
    25501 gcatcctcgt cgatcctgcg cgcggcccgt ggctgggagc gcccctcgga tcatgtgccc
    25561 ctggcagtaa cactcgctct ttgagcctgc gagagcatca tcttgccagg tggatgccgg
    25621 ctggcgggat gttgctctag atgatttcgg ccagccgctg ccgggtgctg gccagttctc
    25681 cggcgagatg ggctctcagc gccgctgcgc tggctggatg ggcctcgagc acctgccgca
    25741 tcagggattg cgacaggacg agcacgcggc tgggttcgag tgccagcgcc gttcccggac
    25801 gttccccggc aaccagaagg gccgatacgc cgatcagccc gcccggaccg acgacttccc
    25861 cttcgcccgg cgcaccatcc gtggcctgaa caccgatccg tccactgacc accacatagc
    25921 cggtatcggc cctggcaccc ttgcggaaca ggtcctcgcc cgccttgagt tgctgtgtgg
    25981 tcgctgaaaa tgcaagcaca tgcagggctt ccggctccag agcacgaaac agggcaaggc
    26041 cggcaagggc cgtggttttc tcgtcaagtc gcatcagagg gtccgctatg gcaccagctt
    26101 gtaaccgcca ccctctgtta ccagcaagcg ggcatgtccg gaattgggtt cgattttttg
    26161 tcgcagccgg tagatatgcg tttcaagcgt atgggtggtg acattggaat tgtagcccca
    26221 tacttcctgc agcaacacgt ctctgggcac gactttctgg tcagcgcggt agagatagcg
    26281 aaggatggcg gtttcctttt cggtcaaccg cagtttcgat cccttggtgg tgaccagaag
    26341 cttggcgccg ggacggaacg tatacgggcc gatcgcaaat accgcgtcct cgctcgccgc
    26401 atgttgacgc agatgggcgc ggatccgtgc cagaagcacg gcgaagcgga acggcttggt
    26461 cacatagtcg ttggcgcccg attccagtcc gagaaccgtg tcggcctccg aatcatggcc
    26521 ggtcagcagc agaatcgggc cacgaaacgc attcttgcgc agcatcttga cggcctcacg
    26581 gccatcgata tccggcagtc cgacatccat caggatcata tcgatctggt ctgccttggc
    26641 gatagccagc gcggcagttg cggtatccgc ttcctgaaca gcaaattcct catgcaggtg
    26701 caactgttca gccagcgcct cgcgcaatgc ggcatcgtca tcgacaatca ggatcttccg
    26761 gatattgctc atcaattgtc cttgcactga accgttggag cctatccaat gtgccatcat
    26821 gcggcaaggt tctgccaccc ggactgcgat gaaaggttat tgcacaacga ttgtggtcta
    26881 tcggcttgag cccagaggcc ctcgcgcgct gctggtggcg ggcccgcttg ccatgccctg
    26941 ccgcatcggt cgcaatgggg cagcctgcgc caagcgcgag ggcgatggcg catcgccatt
    27001 gggaaccttt gcactgcttg gcggctggtg gcgaaacgac cgccgccgtc ggccacagac
    27061 cggcctttgc ctcaccgcga tccggaagga cgatggctgg tgcgatgctc ccggccatcg
    27121 ggcctacaat cggcccgtgc gcctgccctt tgccgccagt catgaaagca tggcccgcaa
    27181 cgatcatcag tatgacgttg tgctcgacat cagctggaac cgccacccgc gccgccaggg
    27241 ttggggcagc gcaattttcc tgcatttgat gagtgagact ggcggcggca cggccggctg
    27301 tgtggccgtg cccgccggac ggatcgaccg gctgctggtc cgcatagggc cgcagacgcg
    27361 gatccggatc cgctgaacaa ccgaatcagc gcgcgccgaa aatcgcgctg ccgatccgca
    27421 catgagttgc gccggactga atcgctgcgg caaaatcggc gctcatcccc atcgacaact
    27481 gatcgatacc attgcggcgg gcaatcttgg ccaagagtgc aaaatgcgga cttggtggct
    27541 gatcggccgg aggaatgcac atcagcccgg caattgtcag cccgtgcttg acccggcatt
    27601 cggcgatgaa tgcgtccgcc tcctcgggca ggacaccggc tttctgcgct tcggccccgg
    27661 tattgacctg cacaaaaagc ctgggcgaat gaccggttct ggcgatggcc gccgccagtg
    27721 ccgccgccag cctgggccga tcgaggcttt cgatgcagtc gaacaatgta accgccgtct
    27781 cggccttgtt ggtctgaagc gggccgatca gatgcaggtc aacatccgga aaccgctgtt
    27841 tcagttgcgg ccacttctgc tgcgcctctt gcacccgatt ctcgccgaac acccgctggc
    27901 cggccagcag caccggctcg atcgcttcga ccggaaatgt cttggagacc gcaatcagtg
    27961 tcacgtcggc ggccgggcgc ccgcaatcct cggcggcacg gcggatcagt ttgcgcacgg
    28021 cgtcgatgcg cacggcgcct ggatgatctt cctggctctc gcccatgccg taaatcctca
    28081 cttggccgac aatcgtatgg gtgttgcagg tgccatacat cgcccgggtt gaccagaaaa
    28141 accgaatgat gtccaagggg ctggtccact gacaagcagc gacccatgcg caacgcccgt
    28201 tacaatcccc gcgaagccga acccagatgg cagaagatct ggaacgaccg caacacgttc
    28261 aggaccagga atgacgattc ccggccaaaa tattacgtgc tcgagatgtt cccctatccg
    28321 tctgggcgca tccacatggg ccatgtgcgc aattatgcga tgggcgatgt cgttgcccgg
    28381 cacaagcgcg ccaagggcta ttgcgtgctc catccgatgg gctgggatgc ctttggaatg
    28441 ccggctgaga atgcggcgat ggaaaagaaa tcccatccca aggcctggac ctatgccaac
    28501 atcgcgacga tgaagggcca gctcaagtcg atgggtctgt cgctggactg gagccgcgag
    28561 atcgccacct gcgatccggc ctattaccgc catcagcagg cgatgttcat cgatttcctc
    28621 aaggccgggc tggtggcgcg caagacctcg aaggtcaact gggatccggt tgatcacacg
    28681 gttcttgcca acgaacaggt gatcgacggg cgcggctggc gctccggtgc gctggtcgag
    28741 cagcgggagc tgacccaatg gtttctcaaa atcaccgatt attcacagga cctgctggac
    28801 gctctcgcca agcttgatcg ctggccggaa aaggtccggc tcatgcagca gaactggatc
    28861 ggccgctcgg agggcctgct gatccggtgg gcgctggaca aggcaccggc cggcattgat
    28921 gctgtcgaag tctttaccac ccgtcccgat acgctgttcg gtgcctcgtt tatcgcggtt
    28981 gccgcggacc atccgctcgc ccgcgctgcg gccgagaagg atgcaaggct cgctgccttc
    29041 atcgaggaat gccagcgcat ggggacttcg gttgccgtgc tcgaaacagc ggaaaagaag
    29101 ggatacgata ccggaatccg ggcggtgcat ccgttcgatt ccagctggac ggtgccggtc
    29161 tatgtcgcca acttcattct gatggattac ggcaccggtg cgattttcgg ctgcccggcg
    29221 cacgatcagc gcgatcttga tttcgccaat gcctacggtc ttgcggtcac cccggtcgtg
    29281 cgcgaccgtg ctctgtccga tgccgaggcc aatgcgcagt ttgaccgtca tgcgcccgct
    29341 gcggtcgatg acggaattct gttcaactcg cgctttctcg atggcctgac ggtcgacgcc
    29401 gccttcaacg aggtggctga ccggctcgaa gcctcccggc ttgccggtga gccggttggc
    29461 cggcgcaagg tcaatttccg gttgcgcgac tggggtattt cgcgccagcg ctattggggt
    29521 tgtccaattc cggtgatcca ttgcgaggct tgcgggatcg tcccggtgcc gaagaaggac
    29581 ctccccgtgg agcttcccga ggacgtgacc ttcgatgttc ccggaaaccc gctcgaccgg
    29641 catccgacat ggaagcgggt tgcctgcccg acctgtggca ggttggcccg ccgcgaaacc
    29701 gatacgatgg acacgttcgt cgattcgtcc tggtatttcg ctcgttttac cgatcccttc
    29761 aatggcacca gcccgaccac ccgcgccgtg gtcgaccgct ggctgccggt cgatcagtat
    29821 atcggcggga tcgagcacgc cattctgcac ctactatatt cgcgcttctt cacccgcgcg
    29881 atgaggaaga ccggtcacgc cgggatcgac gaaccgtttg ctggtctttt cacccagggc
    29941 atggtggtgc acgagaccta caaatcggct gatggggcct gggtggaacc ggcccatgtc
    30001 gagatcgccg ccggccctgc tggccggaca gcaagggatc ggcggaacgg cgctgcgatt
    30061 accatcggtt ccatcgagaa gatgtcgaaa tcgaagaaga acgtggtcga tccggacgat
    30121 atcatcgaga cctacggcgc cgataccgcc cgctggttca tgctgtccga ttcgccaccc
    30181 gagcgggatg tcatctggtc ggaagagggc gtgcaggggg ccggccgatt cgtccagaag
    30241 atctggcgtc tgatctgcga gatcgccgat cggccggcgg accggctggc cgaccgcatg
    30301 ggctcagcac tgctgcggac agcccacaag acagccaagg ccgtcgatga cgatatcgag
    30361 cgcctgcgct tcaaccgctg tgttgcccat atccatgatc tgtcgaatgc gctggccaca
    30421 gcgctgggcg aaggcagcga gaccacgacg gcattgcgcg aggcctgtga aatcatggtc
    30481 cggctgtttg cgccgatgat gccgcatctt gccgaggaat gctgggaggt ccttggccat
    30541 gcaactccgg tttcggaggc acctttcccg gtcattgacg ctgaattgat cattgatctg
    30601 tcgatcacgc taccggttca ggtgaacggc aagaagcgtg ccgacgtgac cgttcccgcc
    30661 gatgccgatc gtgcagcggt cgaaaccgcg actctggcat tggaggctgt ccagcgggct
    30721 ctggaggggc gggcgccgaa aaagatcatt gtcgtgccca agcgcatcgt caatattgtc
    30781 gtctgaggag tcctttcatg gtgtcccgca ggttccctct cgcagcgctt gtcctggtcc
    30841 tgccgctggc cggctgcttt cagccactct acggtccggg tctggcggga tcgaccgaag
    30901 ccattggatc attccagatc gagtcgatgc ccgaacatct tggccatcag ctcaaatctg
    30961 aacttgagtt ccagctgaac aacggcacac ccccgtcgaa ggcgcgtttc cggctggtgg
    31021 ctcaaccgag cggccgcagt gctggtgtca tcgccgatac tgcctctagc aggccccaag
    31081 tgatgagcta tatcgccagt gcccggttct cactgcatga aatcaagacc gggaaagtga
    31141 tcaccagcgg ccaggctcag gtgcaggcct cctacgatcg cgacgtccag cgttttgcca
    31201 cagtccgcgc cctgcgcgat gtcgaaatac gggcagcgat ccagctcgcc gagcagatca
    31261 agatccgtat tatgcctgtt gttgctggcc tgaaggattg acctgtgacc gcgatcaaat
    31321 cgggcgatct ggcccgattt atggcgggcg gttggcagac ctgtccggtt gttctggtgt
    31381 ttggtccgga tgaaggcgct gtgcgcgaga ttgccggcca gcttgtccgg gcagcagcgg
    31441 gcgcagatcc tgatcctctc aattgcatcc ggctggatgg cgagacattg gcccaggatc
    31501 ccgcccggct cgccgatgaa gcacgcacct tcggcctgtt cggcggacgg cgcgtggttc
    31561 atgtgcgcaa tgcggcacgc acccctgcag catcggtgac tgccatcgcc gacgatccgg
    31621 tgcccgatac gctggtgatc cttgaggccg ttgatctcaa atccggtgcg ccattgcgga
    31681 cgctggctga aaaacaccgc tccatcgccg cgatcgcttg ctatgctgac ggacaccgca
    31741 gcctgcaaac tttgatcgag acaaccctgt ccggccacgg attgtcgctg acgcgcgatg
    31801 gccgcgacgc cctgatgggg gcgcttggcg ctgaccggct gttgacccgc tcggaactcg
    31861 acaagctgac gctttatgcc ctcggtgaaa agaccgtcga tgcccgcatg gtcgccgaga
    31921 ttgtctctga tgccggccgt cacgaaacat cgtccctgat cgatttcgcc tttgctggcc
    31981 accttgcgac agtcgagccg gaggcgaatc ggatttttgc caccggaatt catccagcat
    32041 cgctgatgac ccaggtactg tcacacatta tgttgatgcg tcggctgcgc cgcgccgttg
    32101 atcgcggtca atcactggat gccctgttgc ggcaggcccg cctgcatttc tcccgggttc
    32161 cggcagttga ggcggctttg cggtcatgga actgcgaacg tctggatcgt gctctggtcc
    32221 tggcgtctga tgccgtcctg aacagccgca ggacggcccg attggccgac tcgcttgcca
    32281 ttcgtctttt atggagcatt gccagaatgg ccggcaaaga caaagtatct agtagaatac
    32341 aataaaaatg ccttaagagg cttatataaa tactatttgc tatcgattag taataataaa
    32401 aatgaaatat atgaaagatt tttatagttt tgaatatttg aggctagctt gaatttggcc
    32461 aatatcaaat aagaccctcc caaataagac catcccaagt cactgggggt cgggagggtc
    32521 tttgcagctg atcagaaaat caagccccac gcaatcgccg gcacagcgca tccagctgct
    32581 caagcgagcg gtagcgcaca gtgagcgcgc cgccctcccg gcgatggtca atcgataccg
    32641 gcagaccgag gatatcacca agcgcctttt ccagggcccg ggtgtcggcg tccttttccg
    32701 gacgggggcg gggtgaaaca cccgtctcgg cgtcctcaac ctccctttgg gccaggcgct
    32761 cgacatcgcg caccgtcaat ccctgcgcca ggacccgttt ggcaatgccc tccggatcgg
    32821 ccactgccag cagggcgcgg ccatgacctg ccgacagctt gccttcgctc agaagcgatt
    32881 tgacgctgtc gggcaggcgc gacaggcgca acgtattggc aacatgactt cggctcttgc
    32941 caacgacgcg cgacaaatcg gcctgggtgt agccgaattg ggtgagcagg ctttcgtatc
    33001 cgcgcgcttc ttcgagtggg ttcagatcgg cgcgctgaac gttttcgatg atcgccagtt
    33061 ccagcgcctg acgatcatcg gcttcgacaa cgagaatggg cacttcatgc aaccctgccc
    33121 gttgcgctgc tcgccagcgc cgctcgccgg cgatgatctc aaattgatcg gggacgccgg
    33181 agatagcccg gaccagaatg ggttgcagaa ttcccttttc gcggaccgag gccgtcagct
    33241 cctctatctc cagctcggtg aacgttcggc gcggattgtt tggattggct cgcaggaaag
    33301 ccaccgaagc acgccgcggc atccgttcgg accgctcttc gcctccgaca tcaccgatca
    33361 gcgctgccag tccgcgccct agcctttgtc tgtgagtttc gtctaccatt gttacgccta
    33421 tgaatcggat ggctgtcagg ctgctcgcag catgcgctcg cgctggatga cttcggatgc
    33481 aagctttagg taggcctggc tgcctgcaca tttgagatcg tagagcagga ccggcttgcc
    33541 gtgggacggc gcttccgaaa cccgcacatt gcgaggaatg acggtttgat agaccttgtc
    33601 acccagaaac tgccgaacat cggaaatcac ctggttggcc agattgtttc gtggatcaaa
    33661 catcgtcagg acgatgccat ggatgatcag ccgcggattg aggccctgac gaacctgatc
    33721 cactgtcttc agtaactggc tcaagccttc aagggcaaag aactcgcact ggagcggcac
    33781 cagcactgca tcagccgcag ccatcgcgtt gatcgtcaac agattgaatg agggcgggca
    33841 atcgataagg atatagctga atggttcagc ccgagccgat tgatgctcgg caatcgcctc
    33901 gcggagacga agggcgcggt ctttgcgcga agccagctcc agttcggctc ccagcagatc
    33961 aagcgttgac ggcgccagcg aaacccgcgg aacggcggtt gcatggatcg cttcgccaag
    34021 cgtgatctct cctaccagca catcataggt cgactgccgg cgctccttct gggaaatccc
    34081 catgccggtg gaagcatttc cctgcggatc cagatcaata atcagaacgt tttcgccaat
    34141 cgcagcaagc gccgtcccga gattgatcgt cgttgtggtt ttgccgacgc cgcccttctg
    34201 attggcgatg gcgagaattc gggcctgggt catgagcttt cagcagtcct atgttccact
    34261 tggtggggtc cgtcgcctgg ctgaacggac ttccagtatc caggactctg cctccagcgc
    34321 gtgggcatgg cggacaaagt cgatctccca atactgtgcc gcctctgata gctcattgtc
    34381 cagattgcgc cccttgagaa agagcgctct cgacccgctt ttcaacagcg gctcgcacca
    34441 atcaagcaat tgggtcagcg gggcgagggc gcgggcggtt acaatctccg gaactggaag
    34501 gcgcggaagc cattgctcaa gccgccccgt cacaacttcg gcgggcagtt ccagggtctg
    34561 ctgcaccttc gtcagaaaag ccgtcttctt ggcactggaa tcgatcaaag tgatgcgtcc
    34621 gatgccgcgc tccttgagac ggatcgcaat caccaatcct ggaaacccgg cacccgagcc
    34681 aagatccatg aatgtcctgg ccctggggac cagatcagcg atctgcaacg aatcgagaaa
    34741 gtggcgcgtc catacctccg agagcgttgt cttgccaacc agattgattg aaccctgcca
    34801 gcgcctcagc aagtattcgt agcgatccag ggcttccagc gtttcacgtg aaacatctac
    34861 caaggcgcga aaccgctccc ggttcccgct cgaatccctg tcgacggcat cgccctgacc
    34921 gttcattgcc gaccctcgtg cccgcaaccg cccgatgatg gccctgtaaa acgcgacggt
    34981 tcccaccgtc gcgcgtgcgc gcagaccaga gcaagagccg ctggtgtcat gccttctatg
    35041 cggctggcct gctcaatgga acgggggcga atcatggtga gcttcgacgc cagctcgctc
    35101 gacagccctg gcaggccccg gtagtccagc gcgtccggga tggcgatggc acgatctcgt
    35161 tcgatccgct ccagttcctg tttctggcgc ccgagataga ccgcatagcg cgcatcgaca
    35221 ctgacggcct cgcgcacgtc acactcaatt tggtgcagct ccggccacac gtctgtgagc
    35281 cgcgcgatgt caatgcccgg aaggctgagc agatcatagg ccgatcgacg gacgccgtcc
    35341 tgtttgatgt caagccccgc agcccgggca gcattcgggg tgatggtgca ggcctctaat
    35401 accttcgtag ccgaatcgaa tttttgcgcc cagatctcga atgcctcctt ccgttgtttc
    35461 ccgacacagc cgagcgacaa accaatcggc gtcagccgcc ggtcggcatt gtcagaccgc
    35521 agtgacagcc ggaactccgc gcgcgacgtg aacacgcggt agggctctgt gacgcccctt
    35581 gtgaccaaat cgtcaatcat gacgccgata tatgatccct ctcgttcgag caccgcgctg
    35641 tcccggcccc cggcaaccaa ggcggcattg agaccggcca caacaccttg agccgcagct
    35701 tcttcataac cggtcgttcc attgatctgc ccggcaagga agagtcgggg cagacgcttg
    35761 cactggagat cagctgtcaa ttcccgcggg tcaatgaaat catactcgat cgcatatccg
    35821 aactgccgga tgacagcgcg ttccaacccg gggatcgagg cgatcatcgc tgcttggacg
    35881 tcttccggaa gcgatgttga caacccattg ggatagacca gatcgctgca gatcccttcc
    35941 ggctcgagaa agatctgatg cccctcgcgg tcgccgaagc ggactatctt gtcttcgatc
    36001 gacggacaat agcgcggtcc cctcccggag atctggcccg aatacatcgc tgaacgaccc
    36061 agattgcctt ggatcagggc atgcgtatcg gcgccggtac gggtaatgcc acaggcgacc
    36121 tgctccccgc tgatagacgg cgtcaggaag gaaaacggca caggctctgc atcgccgtgc
    36181 tgaagctcca gacggtccca ggcaatactg ccccgatcaa gccgaggcgg cgtaccggtc
    36241 ttcaaacgtc ccagcttcag gcccaaatgc tcgaagcgcc tggacaaggc attcgccgct
    36301 tcttcgccga tgcgcccacc ggactgccga ctttgcccaa catggatcgt gccgcgcaga
    36361 aacgttccgg tagtaatcac caccgcgccg cacctcagga ggcgcccatc gctgagcacg
    36421 acacctgccg caacaccatc actgatcacg agatcggcga cttccccctc aatgaccgtg
    36481 aggccgtcga tggctgcgac ggcctctgtc atggctctct gatatcgccg ccggtcggct
    36541 tgtgccctca acccctgaac tgccgggccc ttgcgccggt tcagcacgcg gaactggatg
    36601 ccggccgcat cggcaatcct gcccatcagc ccatccagcg cgtcgatctc gcgcacgagc
    36661 tggcccttgc ccaaaccacc gatggcggga ttgcacgaca tcaccccgag attgtcccgg
    36721 cgcagggtaa ccaatgccgt ggcagcgccc atgcgggcgg cagcggcagc ggcttctgtg
    36781 cccgcgtgcc cccccccgac gacaaggaca tcgaacgtct gatttgtact catggtcccg
    36841 tccatacgct cctgacctcg gatcgtcaaa atctgccgat ggcaatccta atgtttcgga
    36901 atcgactcga aatgtttcac gtgaaacact acttcccgat gcaaaaccgg ctgtagatcg
    36961 cacccaaaac gtcatcgacc tcgatcctac cgaccagccg atcaagttcg acactcgcct
    37021 gccgaagatc ttctgccaca aactcaatcc ccaaccccgc ctgtcttgcc cgacccaggc
    37081 aggcgagggc ggcttcaatc cgcaaccgct gccgcgttcg tgtcaccagg gccacatcgg
    37141 tgcgtccaac cagcacctcg accgcgcgct taagtgcgcc gagcaactca tcgcaccccc
    37201 ctggtgcaac cgttgacacc cgacaccagg cggaaaacgg cgcctccccg agatccgcct
    37261 tcgacgcaac aacccggaca tcaccacgaa attcgacggg caactcgcgg acgtcgtcaa
    37321 ccggttgcag ccacagcacc aggtccgctt ccctggctgc ctgcctgctc cgcctatttc
    37381 cctcctgttc gatcttgtcc gcactgtcgc gcagaccggc cgtatccgag acgatcactg
    37441 gaatcccatc gaggtcgaga tggatatcga tgcggtcccg ggtcgtcccc ggaacctctg
    37501 aaacgatcgc aacatcgcgt ctggcaaggg cattcaacac actggacttg ccggcattcg
    37561 gccggccgat gatggctacg cggacgccac cccgtattct ctctccacct gcggcagtcg
    37621 ccagcgcccg cttcaattcc ccaataagcg cgtcaacagc ccgttccata tccgaatcga
    37681 gtcgttcggc gacatcaccc tcgtccgaga aatcgatctc taccgcaaca gccgccatca
    37741 cctcgatcaa actgcaccgc cagccctcca cgacatcgcg cagcgcactg ccgccacctt
    37801 caatggcttg cacccgctgt cgctccgtct ctgcatcgat cagatcggcc agcccttcga
    37861 gatccagcag gtccatcttt ccgttgaaca cggcccgccg tgcgaactct cccggctccg
    37921 ccattctcag ccccgggagc gatcccagcg ccctgagcat cgccgcaacg acagccggcc
    37981 ccccgtggca atgcaactcg cccacatctt cgccggtctg gctgttcggt ccggaaaaga
    38041 acaaaacaat cccacgatcg agaacgccgc cggttttcgg gtccctgatc gaccgcaagc
    38101 gggccgattg aaccgaaggg accgacccgc aaagcgtttc gagcgcgaat cgaacctgcg
    38161 gcccagacag cctgactatt gcaaccgcgc tgcgaccctg tcccgaactc aatgcaaaga
    38221 tcgtgtctgc ccgatccagc cccatccctg ctttcccttc tcgtcacttt gatctagcgt
    38281 gaccatcaac cacagaggtc ccatggctcc aaatcggctt gcacaggccc tcagcccata
    38341 cctgctccag cattctggca atccggttga ctggtggcca tggcgcaccg acgctttcga
    38401 gctagcccgc caaacccagc gtcccgtctt tctctctatc ggctacgcgg catgccattg
    38461 gtgccacgtc atggctcacg aaagcttcga ggatccggaa accgcggcgc tcatgaatga
    38521 gcttttcgtc aacgtcaagg ttgatcgtga agagcgccct gacatcgatc agatctatat
    38581 ggccgccctg catgcgctcg ggcaacaggg aggttggccg ctttcgatgt tcctgacacc
    38641 ggacggccag ccattctggg gcggcacata ttttcccaaa acacgccgct atggccagcc
    38701 agcgttttcc gaggtgctgc gatccgtcgc cagagcctac catgccaacc ccgacaccat
    38761 cagaaacaac accgaggccc tgattgcctc cctcgccgcc gctcccgccg aagccggcac
    38821 aatcccgccg cccgatcgcc ttgtgaccat cgcccgggca atcgccgggc atttcgatcc
    38881 gcttcatgga ggtcttgccg gcgcgccaaa attccccaat gcacctgttc tcgacctcat
    38941 tagccgtgcg gccactgcct ccaaagatcc tgccctgttg acgccggtcc tgaccacgct
    39001 tgaacggatg gtccgcggcg gcctgttcga tcagatcggc ggcggctttc accgctatag
    39061 cgtcgatgcg gcgtggctgg tgccgcattt cgaaaagatg ctctacgata ccgcgcagct
    39121 gctgcccatc ctggcgatga ccggtcgcag cacagcctcc gaattattgc tcaaggctgc
    39181 cgatcgctgc gttgaatggc tcaaacgcga catgattgct cctcagggtg gcttcatctc
    39241 cagtctggac gccgacagcc tcactcccga tggtcatcgg gaagagggcg cctattacac
    39301 ctggacgccc gatgaggtca cagcagtgct tggcccggcc gctgccccgc tgtgcaaagc
    39361 ctttgacatc atgtcgcctg gcaatttcga cggccgctcc atccccaacc gcctcggccg
    39421 gacgggcctt catccgcccg cgtcagacaa tcatgatgtc gcccttgacg ccctgctcgc
    39481 tgcccgcctg ctccgcccgc cgccgccgcg cgatgacaag gttctggccg actggaacgg
    39541 tatgatgatc actggccttg cgagggccgc cgacaagctc gatcatccgg actggcgcaa
    39601 gcttgcgacc agtgcctatc gcgccaccgt tgaactgctt gagcccaatg gcaacctccg
    39661 ccatgtctgg cgcggcaagc caagcgtcgg accatgcttt gccagcgatc ttgcgtcgat
    39721 ggcgttggcc gccacagccc tgcacgagtc aaccggcgat ttgaactata tccccgacgc
    39781 cgaacgctgg ctgacaaccc tgatcaccga ttaccgcttg ccatccggac gccttgcact
    39841 gaccgcccgc tccgccgaac cgttgatcca ccgccctcag tcaacccagg acgatgcagt
    39901 gcccaaccac catgcccttg cgatcgaggc catgatccgt ctcggtgctc agaccggcga
    39961 cctattctgg catcagcggg cgtcaagcct gatgtcggcg gtgtcagagg ccatgctcga
    40021 caacccctat ggccacgctt cgctgctctc gatggtcgac tttgccacgc gccgctgtga
    40081 catcctggtg gccggtcaga tcgatgcagc ctttgccgct gccattcgcg ctgttccgta
    40141 tccgaatcga acaatccgtt tcctcacagc agcgcaagca gcggcccatc cgctgaccgc
    40201 agcccttccc tgtcccgacc aacccaatgt cctgatctgc ctagcagatc gttgcctgcc
    40261 gatggcgaca acattacccg aattccgtga tcgcctcgca acagccggct ggctctgatc
    40321 aatcacgcgt attcatcgaa tcgaagaatt cggagttctg cttggtcttg gtcagcttgt
    40381 cgagcaggaa ctcgatggcg tcgaccgtgc ccatcgggtt gaggatacgc ctgagcacat
    40441 acatccgctt gagaatatcc tggggaacca gcagttcctc cttgcgcgtg cccgaccggg
    40501 taatgtcgat cgctggaaag acgcgtttgt cggagacctt gcggtcgagg atgatttccg
    40561 aattgccggt gcccttgaac tcttcgaaga tcacttcgtc catgcggctg ccggtatcga
    40621 tgagcgctgt cgagatgatg gtcagtgacc caccctcttc gatgttgcgc gcagcaccaa
    40681 agaaccgctt gggccgctgc agggcgttgg catcgacacc gccggtcaac accttgcccg
    40741 acgaggggac cacggtgttg taggcccggc ctagtctggt gatcgaatcg agcagaatga
    40801 cgacgtcacg accatgctcg accagccgtt tggctttctc gatgaccatt tccgccacgg
    40861 caacatggcg cgtcgccggc tcatcgaagg tcgaggagat cacctcgccc ttcaccgagc
    40921 gctgcatgtc ggtcacttct tccggccgct cgtcgatcag cagcacgatg agatagcact
    40981 caggatgatt gtgggtgatg gacttggcga tgttctgcaa caggaccgtc ttgccggtgc
    41041 gcggcggcgc cacgatcagc gcgcgttggc ccttgccgat cggagacaca atgtcgatga
    41101 cccgcgccga catgtccttc tttgtcgggt cctcgatctc aagcttgagc cgctgctccg
    41161 gatagagcgg ggtcagattg tcgaaatgga ccttgtgccg cgccttctcc gggtcctcga
    41221 aattgatctt gttgaccttg agcagcgcga aataccgttc cccatcgcgt ggcgaacgga
    41281 tctgcccctc gaccgtatcg ccggttcgca acgcgaaacg ccggatctgg gaaggcgaga
    41341 tatagatgtc atcagggccg gccagataat tggcatcggg cgagcgcagg aagccgaacc
    41401 cgtcctgcag cacctcgaca acgccttcgc cgatgatttc cgtctcccgc gccgcaagct
    41461 gcttgagaat ggcaaacatc agttcctgct tgcgcatcgt gctcgcgcct tcgacctcca
    41521 cctcttcggc aaaggtcagg agctcggtcg gagacttggc tttcagctcc tgaagcttga
    41581 tttccctcat gggttgtcct tcagacaatc tttcggcgtc gttacgttca ggactatgcc
    41641 cgcaaggcca gaacacattc gtccgaattg agacggtctg cggacagacg gcaatccgca
    41701 gaaaggggga tcgccatcac tcttcgttgc gctgccataa cgggttttgc cgaaccggac
    41761 aagcctcatg cagccattca cgccaaccca attgcctcac ccctcaaacc tcagcaagcc
    41821 aatatttgca tatttgcaaa ggctctcttg caaagccaga attcgcttgc catttccccc
    41881 gcagaatttg ccgttaaatt cgcctttcgt cgcagataac cataccaacc tagggagccc
    41941 gcccatgcga tcgaaaggtt cttctctcgt cctcgtcact tgtgccctat tcgctggcaa
    42001 tgcacttgct cagcaacagg ggccgcagcc gaatgccggg atgaattttt tcatctcatc
    42061 tgccagcctt ggcaagggtg gagacctcgg cggtcttgaa ggcgccgacc gacattgcca
    42121 gaccctcgcc actgctgtcg gccagggctc aaagacctgg cgcgcctatc tgtcgaccca
    42181 ggccgccgac ggcaagcccg ccgtcaacgc tcgcgaccgg atcggcaagg ggccctatct
    42241 gaacttcaag ggtgaggaaa tcgccaagga cattgctggc ctgcatgatc ctgcggccaa
    42301 ccggatcaat atcgagaccg gcctgacaga acgcggccag aaggtgatgg gccgcggctt
    42361 cctgatcaac atgcatgacg tgctgaccgg ttcgcagacc gacggcaccg cattccctcc
    42421 tgaaaaggat atgacctgcg gcaactggac caagagtggt gccggcgctg ccatggttgg
    42481 gcaccatgac cgtatcgggt tggatgattc agccccggcc aagagctgga atgcctccca
    42541 tccttcccgc gggtccgaag gcggttgcag catggccgac ttgcgttcca ccggcggcgg
    42601 cggcctgatc tattgctttg cggccaactg accccgagca cctgaaatcg agacagcccg
    42661 gaacgcagcc cgctgccttc cgggttttgc ttgcatcaga acggcttgac aatcaccaga
    42721 atgacaaccg cgatcatcag cacggtcggc acctcattca tgacccggta aaaatacgct
    42781 gtccggacat tgcgatctgc ctcgaaatcc ttgcgccagc gcgccagaaa cccgtgaaac
    42841 cctgacagca gaacaaaaac gatggcaagc ttggcgtgca gccagccgcc tttgaaggca
    42901 aagacgtacc aggccatcca caatccggtc agccacgata cgatcatcgc cggattgatg
    42961 attgctcgca aaagtcgccg ctccatgacc ttgaaggttt ccgattgctg cgagcccttt
    43021 ggagcatcga catgatagac aaacagtcgc ggcagataga gcattcccgc catccaggag
    43081 atcactgcaa tgacatgcag gctcttgacc cacagatagg ccccgtcaac cgtcatagct
    43141 cacctctgac cttctggacc agccgctcca catgggcgat cggggtctcc ggcaatatcc
    43201 cgtgccccag attgaagata tgcgcccgac cgtcaaccgc ttcgagaatc gcctcgatgg
    43261 ccctgtccag agccgatccg ccggcaatca gtgcgaacgg atcgagattt ccttgcgtgg
    43321 tgacagcagg gtccaaagat cgagccaccg cgccaagatc aaccgcagta tcaaccccaa
    43381 cagcatcaac gccggtgcgc tcgacaaact ctcgcagttc ggttgatgct ccccgcggaa
    43441 atccgatgat cggcgctccc ggaacccgtg cccgcacttt cttgaccagc gcttccactg
    43501 gctggaggca ccagcgatcg aattcccagc gtggaagaac cccggcccag ctgtcaaaga
    43561 tctgcacgca atcgacacca gccttgaact gggcaaccag gtagtctgcg ctggcatcga
    43621 ccaatagatc gataagccga tgaaacgctt ccggatcctt gtaggcaaac atccgcgccg
    43681 gcttttgatc cggagtgccg cgccccgcga tcatataggt cgcgactgtg aatggtgctc
    43741 cacaaaaacc gatcaaggcc ttgtctggtt caagtccggc acgaacccgg cgcacagttt
    43801 caaacaccgg cgccagccgt tccagatcaa ccgatgattt cagctcggcc gatcctccag
    43861 ctgcgcggat cggatccaga cgtgggccct ccccttcgac aaatcgcacc gtctgtccca
    43921 acgcatcggg aataacgaga atatccgaaa acaggatcgc agcatcaaag ccaaaccggc
    43981 ggatcggttg cagggttgcc tcagccgcca tgtctggcga ataacagaaa tcgagaaaag
    44041 acggcgcctt ggcgcggatt gcccgatact ccggcagata tcgtcctgcc tgccgcatca
    44101 gccacatggg cgggcgggat ccgggaattc ccttgagcgc atcgagaaat gggccgcggc
    44161 gatgggctct ttcaaagaca aaggaagaca agggaagaga ctcttctgtt tgttgcgact
    44221 cttagatcag accgaaatga tgacagtaag tcatccacag cctatcccca atttttgtgc
    44281 ggatcgcttg atgtcgcaca ctcgccgttt ttaccggttc gtgcggtcct tttcgatcac
    44341 aatcgatgga aagtctagtc atccgcagca gatggcgagc caccgattcc ttttacccat
    44401 tcctcacacc agacatctcc cgttggccta gctgtggaca aaggcggcag gaaatccacc
    44461 gccatcaaca cggcttggca taatccgagg ctggcatcgc ttggagagct cggttacaca
    44521 gggctgtcca cagggacaat ttcgctggcg gttgggatat gggacgcagc ttttttcatc
    44581 ttcatctcgt ctcggactcg accggagaaa ccctgctggc ggtcagccgc gcggcatccg
    44641 cccagtatca aggcatttcg gcgatcgagc atatctatcc gctggtgcgc aattccaacc
    44701 agcttgaccg cgtgctccag cagatcgacc tgacccctgg gattgttctg tacacactgg
    44761 ttgatcggtc gattgccgaa cgtctcgaac acgcctgccg cgaatccggc tgcccctgtc
    44821 tgtcggtact cgagccggtg cattcgctgt tccagtcgta tctgggaatt ggaacgacac
    44881 ctcggcccgg tgcccagcat atgctcaacg ccgaatactt ccgccggatc gatgcgctca
    44941 atttcacgat gatgcacgat gatggccagc tgaccgacgg gatcgaacag gccgatgtca
    45001 tcctggtcgg cgtcagccgg acgtcaaaga cgccgacgag catctatctt gccaatcgcg
    45061 gcatcaagac tgccaatgtc ccgatggttc cgggaatccc gcttccggac atcttgtcca
    45121 gtcttgaacg accgctgatt gtcggccttg tcgccagtcc ggagcgcatc gtgcagatcc
    45181 ggcagaaccg cctgctgtcg ctcaatgccg gcgatgacac ggcctatgtc gaccgctcat
    45241 cggtcgccga ggaactggcc tattcgcgcc gcctgttcga caagtacaac tggccgatca
    45301 tcgacgtcag ccggcgctcg atcgaggaaa ccgctgctgc gatcctcgat ctctatcaca
    45361 agcgccgcga acagatgatt ccagaatgaa caccgcgccg ggcagtctct ggcaggccga
    45421 tgcgcctctg gttctggcct ccgcaagtgc cgtccgccgc gccttgctgg aggctgtcgg
    45481 cgtgcccttt gtggctcaac cggctcggat cgacgagcgc gcccttgaag cggcaagcaa
    45541 gagccgtgat cccgtcgagc ttgcccgatt gctcgccgac gccaaggccc tggcggtcag
    45601 tcaagacatg ccgaaccgct atgttctggg cgcggaccag acgttgtcga ttgccggcgg
    45661 catgctgcac aaacccggct cgcgccgcgc agctgtcgat cagctgatgc gcctgtccgg
    45721 tgcgcgtcac cgcctgcatg cggggttggc actggccatc aacggtcatg tgatctggca
    45781 ccatgtcgag acggccagtc tgacgatgcg accgctctcg gcggcttttg tccacgccta
    45841 tctggaagct gcgggcgatc ccgtgctggc aagtgtcggt gtctatcaga tcgaggcgct
    45901 cggcatgcat ctgttcgagg ccatcgaggg cgatcattcc accatcatgg gcctgccgct
    45961 gatgccgctg cttggccggc tgcgcatgat ggggctggtt ctggagtgaa cgccatgctg
    46021 gtgctcggat tgaccggctc gatcgggatg ggaaagtcga cgacagcgac gctgtttggc
    46081 gccgccggcg ttcctgttca tgatgctgat gcaacagtgc acaagctgta tcgcgggcgg
    46141 gcggcgccgc tcattgaggc gagatttccc ggttcaacca actccggcgt agtccagcgc
    46201 gaccggctgg ccgctgccgt ccttggccgg ccggccgaga tcgaagcgct tgaggcgatc
    46261 gtccatccgc tggtgcggga agaagagatc gcctttctga ctgcggcacg ggcgcgggca
    46321 gcgcgtcttg tcgtactcga cattccgctc ctgttcgaga cgggcgccga ggcgcgtgtc
    46381 gacgcaatcc tggtcgtgac agccccggat tctgtgcaga aagccagggt tctggcccgg
    46441 ccgggcatga cggctgataa atttgccgcc atacttgccc gccagatacc ggacaaggaa
    46501 aaacgccgcc gcagccatgc gctgatcgat acgtcattcg gccttgatgc ggcgcgccgg
    46561 gatgtagagg ccctgttgag ggcattggct ggccatccgg gtcgacggct cgtcgggccg
    46621 gcagtgactt ctggatgagg cagggtatgc gcgagatcat tctcgacacc gaaacgaccg
    46681 gtcttgatcc gcggtccggg caccggatcg tcgaaatcgg ctgtgtcgaa tgcatcgacc
    46741 agtttctgac cggccggacc ttccatcact atgtcaaccc tgaacgatcg atgcctccgg
    46801 atgcctttgc ggtgcatgga ttgtcggacg gttttctcaa ggacaagccg gtatttgccg
    46861 atattgccga tgccttcgtc gaatttctcg gcgaggcacg gctggtgatc cacaatgccg
    46921 gttttgatct cggctttctc aatcatgaat tggcgcgctg cggtcgtccg ctgatcggat
    46981 ccgatcgcat cgtcgatacg ttgctgctcg cccggcgcaa acatcccggc cagtccaatt
    47041 cgctcgacgc cctttgccag cgctatggga tcgacaacag tcgccgcacc aaacatggcg
    47101 cgttgatgga tgccgagatc ctcgccgaag tctacgccga actcagtggc gggcgccagc
    47161 gcagcctcgg cctggccata gccgagaccg ttgagacaaa tgttgtcgcg attgtgccag
    47221 gacaggccta ccagcccccc cctcccctgt taggggaggc tgaacgcgcc gctcatcgcg
    47281 ccctggtcga aagcctcggc ggcaaggcct tgtggcgcga ctatctgggg ctcgatgcga
    47341 accaggaccg cccgccgaaa gcctgacctc aggatggctt gaccggcggc tggccctgct
    47401 gcgactgcaa ttccgcgacg cgctgctgat aaagcgccag aaaatcgatc ggatccatgt
    47461 aaagcggcgg gaagccgcca tcgcgcacgg cattggcaac gatctgccgg gcgaagggga
    47521 acagcagccg cggacattcg accatcagca ctccgccaat gtgttcctgc ggaatgttgc
    47581 gcaggcggaa gacgccgcca tagttgagtt caaacgagaa cagcaggttc ggtttggtcc
    47641 cggccgaacc ttcgagcttc agctcgacct cgtaatcgtc accgccgatt ggctgtgcgt
    47701 tgacattgac ctggatctgg atgttggggg ccgacccgtc ctgattggtg agtgagcgcg
    47761 gcgcccgcgg attttcgaat gacaggtcct tgatgtactg ggcaagcgca ttgagttgag
    47821 gagcagtgtc ctcggtctgg ccggcattgg gagtctggct catcgaaaaa tcctcaatct
    47881 gtcgatcggg atcggcttac acggaaccgc gccatccctc aaggcagaac cttgccggat
    47941 tgaacggccg gtgcgcgtcg ccggcttcgc gaagagacct cacgccactc atttggttcg
    48001 agcgtgatgg tgccctctgc tccgctcgcg tgttgtttgc gacggaaacg gttcatcacc
    48061 cagttgcgca ccgagggcga gaacagggcg agcccggcga cggtcgtcag cataccgggt
    48121 agaaaaatca gcaacgcccc aagagctgca aatcccgtgc tgccaagtcc gccaagcccc
    48181 cgggtcaata ccgccttggg cagcccagac agcaccgaac gcagatgggc caacagcagc
    48241 atgccgccca aaatcccctt gagcgccaga gcggcaagtg tcggcaacac accgaacgtt
    48301 ccggtggcca gccagaacag cacgacctcg acaacgaacc acaggataag ggcccagccg
    48361 atcttcgacc ctgaccaccg aactatccgc ataaccctat ctctcgtttg cctgaacgga
    48421 tgcacctata taaggatgga acccctgact cgcgaggacg gtgttgaccg tttccgcctg
    48481 aaccgatgct gagaggcaac gattcgatga acgattttct cgacatctac accatcgttt
    48541 tcatcgcgct ggcgatcttt gtcattctcc gtctcaaatc ggtgctggga gagcgcacgg
    48601 gcaatgaacg cccgcctttt gatccgtttc aacggccaat gccgccgaag ccggacgatg
    48661 gcggcaatgt cgtgcccctt cccacagccg cccgcccggc accagcgccg gcaccgatcc
    48721 ctgccgcagt ggctgccgag gcgccggcgg acaagctcaa ggggattgcc gaaccctcct
    48781 cggaactggg caccaatctg attcgcgtca tggatgcaga gccgctgttc gagcccaaat
    48841 cgtttctcat gggggcccgg gctgcctatg agatgattgt tctggcattt gcccgcggag
    48901 accggcggtc cctcaaggac ctgctgtcaa aagacgtctt tgagggtttt tctgccgcga
    48961 tcagcgaacg cgaggcgcgt ggcgagaagg tcgagacaac ctttgtctcc atcgacaaat
    49021 ccgatctcac cgatgcctcg ttcaagggcg gcaccgtttc ggtgacggtg cggtttgtct
    49081 ccaagatgat cacggcaacc cgcgacgctg cgggggccgt catcgacgga aatcccgaaa
    49141 agatcgtcga tctcgtcgat gtctggacgt tctcgcgcga ggctggcgcc cgcgatccca
    49201 actggcgcct ggtctccacc cagtccgacg cgtgacctgc gatgaccggt ttatcggcgc
    49261 tgcgttcggc ttggccggat ggctggcagt gaccgctgcg ccggcgtccg gcccgcctgt
    49321 gattgccggc gctcggctcg agtcggtctc gtattcggcg cttgacggct ggcaggacga
    49381 tgaccatgcc gccgccttcg cggccttccg cctgtcgtgc cgtgccattg tcgaagcggc
    49441 tcccgaattg cggctggcgc aacctgctcc gcccgcgctt gttgcggcct gccgagaagg
    49501 cttggctctt gacagccagg atgctgcggc cgcacgacag tttttcgaga caagattcca
    49561 gccgtggcgc gttcgcgcgg atggcggcgc cagccttctg accggatatt ttgagccgga
    49621 agtggcaggc gcgatgacgc caggacagga cttcaaggcc ccagttctgg gccgcccacc
    49681 cgatctggtc acactccagc caggcaattt tgatgaagcc ctgccgccgg gactgacgtc
    49741 agcgcgccgg acgtcagcgg ggcttgaacc ttttccggat cgttcggcca ttctcgatgg
    49801 cgccttggct ggccaggggc tcgaacggct gtatctgaag gacgcggtcg aggtttttat
    49861 cgtccaggta caaggttcgg cccgggtgcg cctggccgac gggcggcagc tgcgactgac
    49921 ctatgccggg cgaaacgggc acccctacac gtcgatcggc cgggcactga tcgcccaggg
    49981 atcaatcgca cccgccgaaa tgtcgctgga gcgcctgact tcctggctgc gcgccaatcc
    50041 ggaggctggc gatcgcatca tgcggctcaa tcggtcctat gtgttcttcg aagtgtccga
    50101 ggtcgatgat cccgcccgcg gacctattgg cggcgccgga atcgccttga cagccgggcg
    50161 cagtctggcg gtggatcgca acctctggcc gtatggggtt ccgatcttca tcggagccga
    50221 tcttgatacc ctggccccgc ccctcgaccg acagcggcgt ttgatgattg cccaggatac
    50281 cggttcggcc attgtcggcc cggcgcgcgc cgatttcttc tggggctcgg gcaacgaggc
    50341 tggccgccgg gccggctgga ctcgccatcc gctcgatttt gtggtcctct ggacgcgatc
    50401 atgaccggcc gccacccgcc acgacgtggc tatctgaccc gcgaggatat gcagctctgg
    50461 gcccatgtaa cccagacggt caagccgttc cgtggccgct cggcgttcgt cctgccggcc
    50521 gatctggtcg agacggcacc ggtcaaggtc acaccgccgg ttgccgaacc tgccccgccg
    50581 gcgcccgttc gcttcctgcc tccgctgcag ccgctcgacc ggcgcctgaa acaacgtttg
    50641 accagaggcc gcaaggacat cgacggggta atcgatctgc acggcatgcg acaggccgag
    50701 gcccatggcg ccctgatcga cttcatccac cgcgcccatc gcgaacaccg ctcactggtg
    50761 ctggtcatta ccggcaaagg cagcacggaa ggcatgtccc tggaagggcg cggcgtgctc
    50821 aaccggttgg ttccgcattg gctgaccgat ccagccatcc gccgccagat catcgggttt
    50881 gaacctgccg gccgcagcca tggcggctcg ggtgcgctct atgtccgcat ccgcaaatcg
    50941 cgcccgccgg cttgacctgc tgccaagctt cgttcagggg agccggacat aaatcaggac
    51001 ctccggcatg atcaagaccc gctacgacct tctgtgcatc ggcaatgcga ttgtcgatgt
    51061 gatcgctcat gccgaagaga cctttctgcg ccgctttggc gttgcaaagg gctcgatgac
    51121 gttgattgac gcgcatctgg ccgaaacact ctatgcggcg atggggcccg ccaccatcgt
    51181 atccggcgga tcggcggcga atacggcggt tggtgccggt tcccttggcg gtcgggcagc
    51241 ctatatcggc aagatccggc aggattcggt cggtgatatc tttgcccatg acatcagagc
    51301 cagtggcgtc agcttcgaca cgcaacgcgc cgaagacggg ccgcctactg cgcgcagctt
    51361 cattctggtg acgccggatg gcgagcgcac catgtgcacc tatcttggtg cctgccagaa
    51421 tcttaccgag gccgacatcg accaagccac ggtcaaggcg gcttcgatta cctatctcga
    51481 aggctatctg tgggatccgc cggcggccaa ggcggctttt cgcaaggccg cggcaatcgc
    51541 ccactccgtc ggccgcgacg tggcgctgac cctgtccgat tcattctgcg tcgatcgcta
    51601 ccgcgacgaa tttctcgacc tgatccgttc cggcaccgtc gatcttctgt ttgccaatga
    51661 gcacgaactg aaatcgctct accagacagc tgatttcaat acggccttgt cagccgtcag
    51721 ggcgcaggcc cggcgcgcag tggtgacccg gtcggcaaag ggagcgcttg cggttgcagg
    51781 ttcggaaacg gccgaggttg ccgcctgtcc gatcgacaat cttgtcgata ccaccggtgc
    51841 gggcgatctg ttcgccgcag gcttcctctt tggatcgaca gccggctaca agccggaagc
    51901 ctgtcttcgc ctgggtgccc tggcggccag tcacgtcatc cagactctcg gcgcacggcc
    51961 gcaggtctct ctgcgtcagc tcgccatcga caatgggctg atctgagcgc aggggccgga
    52021 gccggtcaca gtttgcgcag cgcgacttcc tcgatccggt gatcggcgcc cttgcgcatg
    52081 atcagcgagg cgcgcggccg ggtcggcacg atgttttcgc gcagattgac cagattgatg
    52141 cgctcccaca atccgtttgc cgtcctgaac gcctcgtcct cggacaattc cgcataacgg
    52201 cgaaagaacg acttcggatc gcggaacgcg gtttcgcgca gcttcatgaa gcgctgcacg
    52261 taccagcggt gcaggtcact ctcgtcggca tcgatataga cggtgaaatc gaaaaagtcc
    52321 gagatgaacg gaataggctt gccgtccttc accttccgcg gcccctgcag cacattgatg
    52381 ccttcgaaga tgaggatatc gggacgatcg atcaccgcca cctcatcggg aagcacgtcg
    52441 tagacaaggt gtgaatagac cggcgcctcg acgttgcgct cgccggcctt gaccctggcc
    52501 aggaatttca ggatcgcccc ggcgtcgtag ctttcgggaa agcccttgcg ttccatcagg
    52561 ccgaggcgtt gcagctcggc attgggcaga agaaagccat cggtggtgat caattccacc
    52621 ttgggcgtgt tcgcccagcg tgacaacagc gccttgagca accgggcagt cgtcgatttg
    52681 cccgccgaaa cggaacctgc aacagcaatg atatagggca ccttgccgcc ttcctcggcc
    52741 atcaggaagc gctgggtagc cttgtagagc ccctgcgttg ccgcaacata gagcgacaga
    52801 aggcgcgaca gcggcagata gatctcgatc acatcgtcga gcgagaccgg atcattgagc
    52861 gactgcaact tcttcagatc actgctggtc agcgtcagcg gcgtatcagc acgcagattg
    52921 gcccagacct cgcggttgaa cacgcgatat ggggaaagat cgaccgcaga accgagcggc
    52981 gagacccttg cgtccatcgg cagtcccctc ggattaggac tcagcgcgac cggccagcct
    53041 tttcagccag ccccgactgc gccgtccgcc gttccagttc ctcgagcaca tccttagcag
    53101 aaataccgcg gctgtgcagc aggaccatca ggtgatacag cacatcggcg ctttcgctga
    53161 tcaatgcgcc gcgatccccc tgagcgccgg caatcaccgc ctcgatggct tcctcgccga
    53221 acttcttggc aatgcgggca gggccggccg cgttcagccg tgccgtccag ctttcctccg
    53281 gcgaggcgga agcccgtcgc gcaatgatgg tctcaaggtc ggacagcgtg aacggcatcg
    53341 gctagtctcc tgggttgcgg cgaaggcagg ctcaatccat ccgcatcggc aggccggcgg
    53401 cggccatgtg gctcttggcc tgacggatgg tgaaggtgcc gtagtggaag atggaggcgg
    53461 cgagaacagc gctggcgcct ccctgccgca cgccatcgac cagatgatca agcgtgccca
    53521 cgccgcccga ggcaatgacc ggaatggtga cagcatcggc aatggtgcgc gtcagtacgg
    53581 tatcaaagcc gttcttggtg ccgtcgcggt ccatcgatgt cagcaggatt tcccctgctc
    53641 cgtagccggc aaccaatgtc gcgaaggcga ccgcgtcaat ccctgtgggg gtgcgaccgc
    53701 catgggtaaa gatttcccag cgcggcgtct ctccggggcc cgagacctgt ttggcatcga
    53761 tggcgaccac gatcgcctgg gcaccgaacc gttcggacgc ccgggcaacg atggtagggt
    53821 cgcgcacagc agcggtattg atcgacacct tgtcggctcc cgccagcatc agcgcgcgca
    53881 catcctcggc cgtccttaca cctccgccga ccgtcagcgg catgaagcag ttttccgccg
    53941 tcctctgcac gatatcaagc aaggtgccgc gtcgctcgtg actggcggtg atgtcgagaa
    54001 agcacaattc gtctgcgccg gcagcgtcat aggcgcgcgc ggcttcgacc ggatcgccga
    54061 catcggcaag atcgacgaaa ttgacgccct tgaccacacg gccgtcgcgc acatcgagac
    54121 aggggattac ccgaaccttg agcgtcatgc cgccctcgcc gccttgacca gccgcaaggc
    54181 ctcggtcgga tcgagtcgcc catcgtagag cgcgcgaccg gtaatcgcac cggcaagctg
    54241 cgcactttcc ggcgcgacca gccgccggat gtcatccatc gaggcgagcc cgcccgaggc
    54301 gatcaccggg atcgccaccg cttcagccag tgcgatggtt gcctcccagt tgacgccgga
    54361 gagcatgccg tcccgcgcaa tatcggtata gatgattgca gccacgccag catcttcgaa
    54421 gcgccggccg agatcgacag ccgacaggga cgaggtttct gcccaaccct cgaccgcaac
    54481 aaaaccatcg cgcgcgtcga ttccgaccgc aatctgcccc ggaaacgcca ccgccgccgc
    54541 cctgacaaaa ccagggtcgc gaaccgccgc cgttccgatg atgacgcgcg ccacgccctt
    54601 gtcgagccag gcggcgaccg ttttgagatc gcggataccg ccgccgagct gcaccggcat
    54661 gcggattgca gcaagcaccg catccaccgc cgccgcattc atcggccggc cggcaaaggc
    54721 gccgtcgaga tcgacgatat ggagcgcctc aaacccctgg gcctcgaaat tgcgtgcctg
    54781 tgctccagga tcgtcattga agacggtcgc cagatccatg tcgccctgcc gcaggcgcac
    54841 gcactggccg ttcttgaggt cgatggcggg atagaggatc acggcgccca catcaggaaa
    54901 ttggcgaaaa tggcccggcc gagcgtctgg ctcttctcgg gatgaaactg gctgccggcg
    54961 atcggccctc gcgccaccat cgcggtgacc tgcccgccat aatcggcgac ggcaagcata
    55021 tcgctgctgt cggcggcaac cagatggtag gtatggagga aataggcatg caatccgtcg
    55081 ggccccaacc ggaccccgtc gatcagcgga tgtttgcgca gcggacgcag cgtgttccag
    55141 cccatatgcg gcaccttgaa gcccgcgccg tcaggctcca gatgtttgac atggccgcca
    55201 agccagccga gcccggctgt ctcgccatgc tcctcgccga tatcggcaag cagctgcatg
    55261 ccgacgcaga tgccgaaaaa cggtcggccc ttgccacgga ccgcatggtc gagtgcctgg
    55321 tccagcccgg aaactgcagc gagctgaccc cggcagtcgc ggaatgcccc atcgcccgga
    55381 aggacgatgc ggtcggcgct tgccaccaca tccggatcgg ccgtcacgat cacctcgccg
    55441 gtccggccaa tgtcggccag ggcccgctcc agcccgcgat gggcggaatg caggttgccc
    55501 gcgccgtaat cgatcagtgc aaccttcaca gccggccccc gggagctggg aagataccaa
    55561 gcaccgagga gccggccgcc gggtatggca tcgtgggact tggcatcgcg gcacttggcg
    55621 cggcttgcat cggccggtag ttcgagaaaa accgcacttc tgcttcctcg agcgttcgcg
    55681 ccgccaccac ggccacggtt ggtttgccat tgcgctcgag agtccagcgg cgcaaggcgc
    55741 ccgcttcgag cccgatcagg gcatgaacca gaaggctcat agccgattgc accggtcccg
    55801 gcacggaaag cgcatggccg atcccggcca gcagcgccag cacgacgagg acgcccgcca
    55861 gcgccagcca caaccggttg aatgccagcc aaaaggcgcc aaaaaccaag gccggcattg
    55921 aaaaccgctc gctgacgaac tccgcgcctt caagtgcgtt ctcgctcgcc gcggtgtcag
    55981 ccaggtgaac cgtgaagctc tgcatcagac cgacaaagtc cccttggtcg aaggcacgag
    56041 gtcgcgggcg cgaggatcaa gctcgaccgc cgcgcgcagt gccctggcca ggcccttgaa
    56101 gcagctttcc gcaatatggt ggttgttgct gccataaagc gtctcgacat gcagggtcac
    56161 gccggcgttg atggcgaagg cctggaacca ttcgcggacc aattcgcaat caaaactgcc
    56221 gatcttgggc gcgttgaact cggtgcggaa caccaggaac ggccgcccgg aaatgtcgag
    56281 cgccacacgc gtcagggtct cgtccatcgg catgtgcaca tcggcatagc gggtgatgcc
    56341 cttcttgtcg ccaagcgcct gggcgaaggc cttgccgagc gtgatgccga tatcctccac
    56401 cgtatggtga tcatcgatgt gggtatcgcc ctgcgccgaa acggtcagat caaacagggc
    56461 atgccggccg agcaatgtga gcatatgatc gagaaagccc acgccggtgg tgatctcgct
    56521 gcggccggag ccgtcgagat tgatcgcaac atcgatatcg gtttcgccgg tcgtgcggcg
    56581 gaggctggcc ttgcgcatgg ttcggtgcct tcgagcagga atggaccatg cgctttagaa
    56641 catgatgcga caaaagggaa ttcggctttt tccgcttccg gaggaggccg tcttcgggcc
    56701 gtcggccaga gcccgcaggc tgtggggttc aaaatggtgc gtgcgaggtc tcgagcaggt
    56761 tgtctttaga cggaaccatt ccgcggtgag aaatttggcc gaagggccgg gaaagacccg
    56821 aagtgcatac atcggtatgg tcgagggggg gtcggaaatt tcggccagat tcctcccgcc
    56881 cttcgggttt ccgggcggtg ggcgcccaat ttggacgccg gctcgaccgt atccaaagat
    56941 acgctcttcg ccagcctccg cctggatcgc cgcaccgccc gagaagcaga attgattccg
    57001 tctaaagaca acctgctcta tatgcagggg taggcgacga gggttcgatg caggctcatg
    57061 atcacggcca gcccggctgg cacgcaacca ccatcctgat ggtgcgcaag aacggcgaaa
    57121 ccgtcatcgg cggtgacggg caggtgtcgc ttggcgccac catcatcaag ggcaatgcgc
    57181 gaaaggtgcg tcgacttggc aaggggcagg tgattggcgg ctttgccgga gcgaccgccg
    57241 atgccttcac cctgttcgag cggctggagg ccaaactcga gcaatatccg ggacagctca
    57301 cccgcgcctg cgtcgaattg gccaaggact ggcggacgga ccggtatctg cgccgtctgg
    57361 aggccatgat gctggtggct gatcgcgagg cgggcctggt gctgtcgggc acaggcgacg
    57421 tgcttgagcc ggaaggcggg gtgatgggga tcggatcagg cggcatgtat gctctggcgg
    57481 ctgcccgtgc cttgatggca accgatcacg atgcggagac gatcgtgcgc cgtgccatgg
    57541 cgattgctgc cgatatctgc gtctatacca atcgcgaact tgtcgttgaa aagctctgag
    57601 cccctgcccg atgactgatt tttctccccg cgagatcgtt tccgaacttg accgtcacat
    57661 catcggccag tcaccggcca agcgggcagt ggccattgcc ttgcgcaatc gctggcgccg
    57721 ccagcaattg agcggtccga tgcgcgagga ggttctgccc aagaatatcc tgatgatcgg
    57781 gcctaccggc tgtggcaaga cggagattgc ccgtcgcctt gcccggctgg ccggcgcccc
    57841 gttcctgaaa gtcgaggcaa ccaagttcac cgaggttggt tatgtcggcc gcgacgtcga
    57901 gcagatcatc cgggatctgg tcgaagtggc gatctcgctg gtgcgcgagg ccaggcgcaa
    57961 ggatgtgcgg gccaaggcgg aactggcggc cgaggaacgt gtgatcgacg ctcttgtggg
    58021 cgc
//
LOCUS       DAOMXG010000002        37629 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_756_length_37629_cov_13.964763, whole genome shotgun sequence.
ACCESSION   DAOMXG010000002 DAOMXG010000000
VERSION     DAOMXG010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 37629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37629
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_756_length_37629_cov_13.964763"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(185..2326)
                     /gene="glgB"
                     /locus_tag="PLQ11_00310"
     CDS             complement(185..2326)
                     /gene="glgB"
                     /locus_tag="PLQ11_00310"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019857059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HRK23372.1"
                     /translation="MHDDPFAVLGPHRHGRGTVQRAFTPGAETLRATTLSGEDLGVLE
                     RHHADGFFEGRIASPGKDQPVRLVATGAAGEWAFVDPYSFGPILGPLDDYYAAEGTHL
                     RLFDRLGAHVLMHQGVTGTHFAVWAPNARRVSVVGDFNLWDGRRHLMRRRIASGLWEI
                     FLPDVAAGAPYKFEIIGADGHLRLKSDPYAFAGEKRPANASIITAPQPFTWTDQEYRA
                     RREGLDQRRVPVSVYEVHAGSWRRGEDGGFPSYDMLADTLIPYVSDMGFTHIEFLPLS
                     EHPLDASWGYQPTGLFAPTARFGPPEAFARFVDRAHAAGLGVILDWVPAHFPTDAHGL
                     GWFDGTALYEHADPRQGFHPDWNTAIYNFGRKEVASFLINNALFWLERYHIDGLRVDA
                     VASMLYLDYSRKAGEWIPNREGGRENLEAIAFLRQLNTIVHAQHPGVLMIAEESTSFS
                     GVSQPVHLGGLGFGFKWNMGFMHDTLAYLKRDPLHRKHHHSDLTFGLIYAFSENFVLP
                     LSHDEVVHGKASLLHKMAGDDWQKFAHLRAYYGFMWGYPGKKLLFMGQEFGQRREWSE
                     ERGLDWELMQHAPHDGLRALVRDLNRLYSATPALHARDCEPEGFEWLVVDDAARSVTA
                     WLRRDGTGGMVAVISNFTPVPRDGYVLALPKAGRWIERINTDAALYGGSGLGNLGAVK
                     AVAKPLHGRPAQARVTLPPLSTIILELETSS"
     gene            2548..4071
                     /locus_tag="PLQ11_00315"
     CDS             2548..4071
                     /locus_tag="PLQ11_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008196340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HRK23373.1"
                     /translation="MSFPKPSSALVPNTAAFESLPLVKPTGFREYDARWWFGHQGSPK
                     APELNLMGVQAVGLGIGTLARRRGVAMEIVTGHDFRSYSGSIKTALIAGLMASGMRVH
                     DIGLALSPMAYFAQFALDVACVAMVTASHNDNGWTGVKMGIERPVTFGPEEMAELRDI
                     VLGGHFHLSGGGSYVFHQNFAERYIGDLTERPKLRRKLKVIAACGNGTAGAFAPQILE
                     RIGCEVIPLDCTLDHSFPRYNPNPEDMHMLHAIAEAVRTHGADVGLGFDGDGDRCGVV
                     DNTGNEIFADKIGVMLARDLSAVHGPSQFVVDVKSTGLYHSDPRLQAMGARTDYFKTG
                     HSYIKRRVRDLKALAGFEKSGHFFFNAPIGRGYDDGLVTAIAVVDMLDRNPDRSMAEL
                     YADLPKTWGTPTMSPHCDDEIKYQVVDRVIARVKAMQAAGETIAGARITDINLVNGIR
                     IGTEDGTWGLVRASSNKPELVVVVESPVSEARMRAMFAAVDSVLRQNQEVGAYNQSI"
     gene            4118..5530
                     /locus_tag="PLQ11_00320"
     CDS             4118..5530
                     /locus_tag="PLQ11_00320"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HRK23374.1"
                     /translation="MTKQIVPVILCGGAGTRLWPASRDSYPKQFLTLNSRRSLFQDTL
                     ARVAGAGYGRPIVLSGNAYRFLVADQAQALGIAADIVLEPARRDSCAAIAAAAAVAVS
                     RDPEAVVLVLSADHDVPDQAAFQAHVARGLAGALLGRIVTFGIQPTFPATGYGYIRPG
                     PALPVPGIHAIAAFVEKPDLATAERYQKEGYLWNSGNFLFRAQVFLDELKALAPDIHD
                     PVVESVAAASRDLDFLRLSEPAFTRARATSVDYAVMEKTALSAVVPSDFRWSDIGSWA
                     AVADMAEKDAAGNSAHGDAMFENARNCYIYSPDTLTSVVGLDNVVVVATRDAILVTDR
                     SNAEDVKKLVGRLSSAGRREAVEHLKNYRPWGHYEQIDRGARYQVKRITVAPGAKLSL
                     QSHFHRAEHWVVVTGTARVTVDDKVHLLGENQSIYIPLGAVHRMENPGQIPLELIEVQ
                     SGSYLGEDDIVRYEDDYNRS"
     gene            complement(5574..5882)
                     /locus_tag="PLQ11_00325"
     CDS             complement(5574..5882)
                     /locus_tag="PLQ11_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23375.1"
                     /translation="MRLLDLLAKLGILRWGWKAGVYRSGRDRPVEFLLDDVYNAKRDL
                     TTKEDLKDMARMVGLSPKQEAASPGEARRCPVCARDVSLAARFCPGCGQQMPNTHQKN
                     "
     gene            6218..6538
                     /locus_tag="PLQ11_00330"
     CDS             6218..6538
                     /locus_tag="PLQ11_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003604694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRK23376.1"
                     /translation="MPEITFVAADATYQTVKGRSGETLMETALRHAIPGIDAECGGAC
                     RCATCHVFVDEDWIDRLPPRTDEEEDMLDTVSSVRKTSRLSCQIRVTAELDGLIVTTP
                     RRQG"
     gene            complement(6687..7619)
                     /locus_tag="PLQ11_00335"
     CDS             complement(6687..7619)
                     /locus_tag="PLQ11_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006937102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HRK23377.1"
                     /translation="MKVIDSHTAGEPTRLIVAGGPDLGRGTMAERRARFQRDFDHIRS
                     FVVNEPRGYDALVGGLICEPVDPTAAAGIIFFNNVGMIGMCGHGTIGLAVTLAHLGRI
                     GPGRHRIETPVGEVAFELIDAHQVRITNVASYRHAKGIAVKVPGLGRVVGDVAWGGNW
                     FFLTETVPCPIKAAHLGELTAAATAIQQALDAQGIRGADGGRIDHVEMFGPPGAPGAH
                     SRSFVLCPGGAYDRSPCGTGTSAKLACLAADDKLAPGTTWVQESVIGSRFEASYQPGS
                     GGTIIPSITGRAYITGEAILLRDPADPFGDGIRF"
     gene            complement(7690..8250)
                     /locus_tag="PLQ11_00340"
     CDS             complement(7690..8250)
                     /locus_tag="PLQ11_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018859310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HRK23378.1"
                     /translation="MTEPSLRLPDDPEPGFPPPKQPGSREPAGSFASLIKLGLSLVIV
                     VAVSWLGQLGTMRGLVDWYPGLAKPSFTPPNIVFPIVWTGLYGLMAFSLWRLLRWPAA
                     TPGRDKALAVLAVQLGLNVLWSWAFFAARNPLAGLFVILALELTILWMIVVIAAVDRT
                     AAQAQIPYAAWVGFASLLNAAIWWLN"
     gene            complement(8262..9134)
                     /locus_tag="PLQ11_00345"
     CDS             complement(8262..9134)
                     /locus_tag="PLQ11_00345"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRK23379.1"
                     /translation="MLERVAPALFVLIWSTGWIVAKYAAVAGADPLTFLVVRYAIAAI
                     VIGLFAFAVGADWPRRRLDWVHGLVSGILLHAIYLGGVWWAIGTGLPAGLSALIAALQ
                     PLLTIMIARFVLDERVSLANLAGVVIGLVGVGIVLAPRVSSVTSETVAAMSGPILVNI
                     VSMVAVTIGTFYQKRFVAAGDLRSLTAVQYLGALMVTLPVALYLEPLNLPFTREVLYG
                     LVWSVLVLSIGAISLMLMMIRRGAVSKVASLIYLIPPTAAVQAWAMFGETLATGQIAG
                     MVVTAIGVYLATRP"
     gene            9427..9747
                     /locus_tag="PLQ11_00350"
     CDS             9427..9747
                     /locus_tag="PLQ11_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23380.1"
                     /translation="MAGSLIKSMGIDLLSPQSLIVIGFALAVFCTSCGWLAHFIAREI
                     SFGMLGNPVVMFVSLWLGLIAFHALAGPLPQHAPLTIAYVAVASAIGGLLIASTLKGQ
                     PWRT"
     gene            complement(9853..12468)
                     /gene="clpB"
                     /locus_tag="PLQ11_00355"
     CDS             complement(9853..12468)
                     /gene="clpB"
                     /locus_tag="PLQ11_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018045319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HRK23381.1"
                     /translation="MNLDRFTERARGFLQAGQAIALRDGHQNFTPEHLLKALLDDEQG
                     MANGLIQRAGGQPEEVRKANDAALAKMPKVQGSGAGSIYLTAPLGRVLAAAEAAAEKA
                     KDSFVTVERILLALAVEKDTEAGKALQRGGVTPQALNGAIEAIRKGRTADSATAENQY
                     DALRKYARDLTEAARTGKLDPVIGRDEEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIV
                     EGLALRIVNGDVPESLADKALLALDMGALIAGAKYRGEFEERLKGVLNEVTAAEGSII
                     LFIDEMHTLVGAGKADGAMDASNLLKPALARGELHCVGATTLDEYRKHVEKDAALARR
                     FQPVFVSEPTVEDTVSILRGLKEKYEQHHGVRITDSALVAAASLSNRYITDRFLPDKA
                     IDLVDEASARLRMQVDSKPEDLDSLDREIVRLKIEQEALKKESDTASKDRLQRLEKEL
                     AELEEKSAALTTAWQAEKSKLGKAADLKKSLDQARNDLAIAQRKGEYQRAGELAYGVI
                     PGLEKQLAEIEARGGGMLEEAVTSDHIAAVVSRWTGVPVDRMLEGEKDKLLRMEVEIG
                     KRVVGQEEALAAVSTAVRRARAGLQDPNRPIGSFMFLGPTGVGKTELTKALAAFLFDD
                     DTAMVRLDMSEYMEKHSVARLIGAPPGYVGYEEGGALTEAVRRRPYQVVLFDEIEKAH
                     PDVFNVLLQVLDDGRLTDGQGRTVDFRNTLIIMTSNLGSEFLVNQKEGEETDAVRDEV
                     MAVVRHHFRPEFLNRVDEIILFHRLRREHMGAIVDIQMARLGKLLAERKISLDLDEAA
                     RAWLAEAGYDPAYGARPLKRVIQKALQDPLAAQLLAGAVRDGETVPIRVGAGGLMVGS
                     EPAAPARRPKNAALN"
     gene            12620..13381
                     /locus_tag="PLQ11_00360"
     CDS             12620..13381
                     /locus_tag="PLQ11_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HRK23382.1"
                     /translation="MLIHVTRLTQYPVKGFTGADLPRVQLVAGDHFPGDRMFAIENGP
                     SGFDPAAPVHQPKFKFLVLMRMPALARLTGRYDAATNRFTLLQGDREVAAGELTTSEG
                     RKTIEDYLAATLAGELRGPPRILTAPPGFRFMDSQSGYISILNLASLRAIAARAGRTI
                     LAPERFRANIITDGLPPWGEFDLIGKTIRIGDVTLRMIKRIDRCAATHVDPVRGIRDL
                     DLVGLLEREFGHHDCGVYAEVVTGGLVSVGDRILA"
     gene            complement(13397..14191)
                     /locus_tag="PLQ11_00365"
     CDS             complement(13397..14191)
                     /locus_tag="PLQ11_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4167 domain-containing protein"
                     /protein_id="HRK23383.1"
                     /translation="MRGRNRRGPNPLTRSYESNGPDVKIRGTAAHIAEKYVSLARDAH
                     ASGDPVAAESYLQHAEHYLRLIAAAQQQQMQAQQLMQGGRVDEADDGMDGDDEFESSV
                     MDRFTFRAPQIQPPPGYVDPNGEQPRHEFAPRPDQPRMEHGRQDQPREHRGDFRRDRN
                     NGDFRRDRGFDRNQPRPDRPQPEAADDEGGLPAFLTAPVRIPIEAGDGPAPGGFAEAG
                     DNAQSGDEGGRFPVRGRRRRHRRGPREDMASGEGPAGEDAQPAISE"
     gene            14795..15694
                     /locus_tag="PLQ11_00370"
     CDS             14795..15694
                     /locus_tag="PLQ11_00370"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRK23384.1"
                     /translation="MKSAFLFASTALAIAAMPALASAQGLRLTPAPGAVTPVPHPVPA
                     AASGWYLRGDVAANANQIGSLKQDNLTLNGGSFLSKSASNTASVGVGVGYRFNDTLRV
                     DATWELRTGSNLKAVDNVRILNSRGQTAADIYSVYDGNIGTQVALLNAYIDLANWNGL
                     TPFIGASIGVARNSISGLTTSNASTLNIYSATAPYDLTSRISDASSGYSPDRVRYSFA
                     WGLTAGLAYAVSDRLTLEMAYRYLNLGNGAQTSLIHCTCGTIGTPLKVGNLSSHDAKL
                     GMRWTFSDTPRVMSHYQPVVAKY"
     gene            complement(15722..17815)
                     /locus_tag="PLQ11_00375"
     CDS             complement(15722..17815)
                     /locus_tag="PLQ11_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HRK23385.1"
                     /translation="MGTPGTAAPEARPLFGYRKHWAQRFGTAPFLPMSRAEMDALGWD
                     SCDVILVTGDAYVDHPSFGMAIIGRLLEAQGFRVGIIAQPDWQGADPFKALGKPNLYF
                     GVTGGNMDSMVNRYTSDRRLRHNDSYTPGGEGGRRPDRAVIVYAQRCREAYPDIPVVL
                     GGIEASLRRIAHYDYWSDKVRRSIVIDAKADLLIYGNAERALVEVTHRIARAGIRQDF
                     SDIRGLALVRSGVPDGWCEAHADDLDDPDEGVRRKERQDQPTVLRLPSFEQVSTDREA
                     YARASRVLHKESNPGNALPLVQAHGNRELWLTEPPIPLSTAEMDGVYELPYARAPHPA
                     YQGKKIPAWEMIRHSVTIMRGCFGGCSFCSITEHEGRIIQSRSEGSILKEIETIRDRT
                     EGFTGIISDIGGPTANMYRIGCKDKETEAICRRPSCVFPDICKNLNTSHEALIQLYRK
                     ARAVPGVKKVMVASGVRYDLAVESPAYVKELVEHHVGGYLKIAPEHTEEGPLSKMMKP
                     GIGSYDAFKALFDAAAKKAGKQYFLIPYFIAAHPGTTDEDMMHLALWLKKNRYRADQV
                     QTFLPSPMALSTAMYHTGVNPLKPVRRGASDPVETVRGLRQRRLHKAFLRYHDPENWP
                     VLREALKAMGRADLIGPGKHHLVPNWQPAGTGSAGEGRRVRSKGQFPKDMKAGSRPFL
                     TKGVPIKPSRRLRPD"
     gene            complement(17956..21078)
                     /locus_tag="PLQ11_00380"
     CDS             complement(17956..21078)
                     /locus_tag="PLQ11_00380"
                     /inference="COORDINATES: protein motif:HMM:NF025611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRK23386.1"
                     /translation="MFDAALTGALIDHGQPEGCCGLADDGGMSLGNSLVQASQNPGEH
                     TSTTTDSLVANEVAAQSGGGALYVIDANVDQKDVLIAGLPAGADILMIDSSRDGVVQL
                     AEALSSRDGVAAVHIFAHGTQGELSLGASVLNAETMNGLYRSTLTGMGTHLSENADIL
                     VYGCNFADGTPGEAAAGLLASLTGADVAGSDDLTGAARLGGDWDLEYVSGSLETDALV
                     FAAYDATLAPPVAVSETYATAHNTPVSGDVSPNDTDPESDPLTFSVHSQPAHGSVTLN
                     SNGTFTYTPNNGFGGEDFFIYRVTDNTSSNPNFANASVTIVVAAAPNAPPDAVNDSYS
                     TNVNTAVSGDVCANDSDPDGDIVSHSLTTNPSHGTVTLNSNGTFTYTPNTGYTGTDTF
                     TYTSNDGNGGTDTATVTITVSGPSNSPPNAVNDSYSTNVNTAVSGDVCANDSDPDGDI
                     VSHSLTTNPSHGTVTLNSNGTFTYTPNTGYTGTDTFTYTSNDGNGGTDTATVTITVSG
                     PSNSPPNAVNDSYSTSVNTAVSGDVCANDSDPNGDILTHTVATGPSNGTVTLNSNGTF
                     TYTPNTGYTGTDTFTYTSSDGRGGTDTGTVVVTVLATNTNPVIAAPMASQANVDGASI
                     TLNLATQVSDADSPVLVYSATGLPPGLSINPLTGMVSGTIDRLASQVGPTSNGVYSVT
                     LTVLDLSGGSATQTFTWTVTNPAPDAVNDAYSVNSGTVLTGSLAPNDSDPDGDSLTFC
                     ATTAPANGTVVVNANGSFTYTPNAGFVGTDSFTYCLTDSQGASDSAVATITVIEVNSA
                     PVAVGSVPTQNNLDNGTATLTMSSYFSDPDSNPLTYTVTGLPTGLSIDPTTGVISGTI
                     DRNACAGGPGGNGVYAITVTATDPRGLSAVQSFAWNVTNPGPLAVADNFATTGSTTVT
                     GTVATNDSDPDGDALTFALVSQPTGGTLTFNPNGTFSYTARDLFVGTDSFTYRVTDAN
                     GATATAIASIAVAAPAVAPVSVTCINTFGDLSHLRSLLAGDGAITTPPPTTTPSFSLN
                     GATFRPWWLG"
     gene            complement(21430..23574)
                     /locus_tag="PLQ11_00385"
     CDS             complement(21430..23574)
                     /locus_tag="PLQ11_00385"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRK23387.1"
                     /translation="MNAATVSVPLAGQANIPPTALPRLRAELEFSEAAPDRLGQPGLL
                     IVDPARNAYFQLAWPASAIVRLWRPIAAAELATDLERQLGLSVSAADIGAVTEFLYRN
                     ELTEADRSQSWRGLAAQREARASHPLIAAMQSFLFLRLPLFNPETLLGWMTPRLAPLF
                     SSGFLVACAGALLVAGYLIARQWSAFTGDLSQLFRLDALPIYALVLFGMKIVHEFGHA
                     VVAHRAGCRVPTMGLALMLGVPVLYTDTTDSWRLTRRRQRLAIVLAGVGAEAILAVLA
                     LLMWPFLPEGTMRLMAVAVATMAVTTSVLINLNPCMRFDGYFALSDLLNVPNLQDRSF
                     ALTRDHLRDLLLGAPRAPAPDLSDALRRALVVFGYVTWIYRLGLYLGIAALVYALSFK
                     LLGIVLFLFEILYFVVRPVVREVADWWTMRERLSTQRIRAFAAGLGLLCCLAVVPWNR
                     IVEAHAVLSARNDLPVHARSPARIADLMVADGDVVVEGQMLARLESPLLMTQVRRIDA
                     EIQALELRLSRAPALDSDRDALPVLETQLRSAREKRAALGRLAQDLEIRAPASGTVVD
                     LDPGLALGVWVSPAQELARIVADRGVRAYALTEEIDARRLQVGANAVFVSETGLRRPV
                     SLKVTSVAEATEASLGEPVLIDRHGGPIAVTEDRRKAVPRQALVAIALEGEGAAPLLL
                     DRGTIRIAAAGENPAARILRRIGQVLQREGGF"
     gene            complement(23578..24873)
                     /locus_tag="PLQ11_00390"
     CDS             complement(23578..24873)
                     /locus_tag="PLQ11_00390"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRK23388.1"
                     /translation="MALTTGARHLAGYDCALLVECVTATDGSLSRKDGCSWRVTAVSG
                     TASVDPEAPLVRAVTEFIATVADSAPSDTKAVQGLDLARQAPNSELAERTRAFPHLLW
                     VPLQTGDGRFIGGLAAFRSTLWPADRRVGLAALGEPFAHAYAALAGNGIGLRPGLRRM
                     VASGRLMLMVPMVALAVLLFPVRFSVLAPAEVVGSQPTLISAPLDGIVAMIAVSPGDP
                     VKKGDKLFGFVDTKLRNDVEIAQKNRAVAVARFQRVIQSAVANHRDSHEIAVARADLD
                     VADAELALANDLLQRVEVRAPRDGVAVFGAKTDWIGRPITTGERVMDIVDPSDVELRI
                     DLGVADSLTLEPGMGVRLFLDGQPLGARSASLTRIGYRPTPGPDQQMVYRIFADFPEA
                     SPAPRLGARGTARLDGSLVPLGFYLFRRPIAALRQKLGI"
     gene            complement(25038..25985)
                     /locus_tag="PLQ11_00395"
     CDS             complement(25038..25985)
                     /locus_tag="PLQ11_00395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRK23389.1"
                     /translation="MSEPSASLWPTAALTIASLLVVTQLVPALWTDEPPPPVRQVQTR
                     KAVPQSARQDPVKSSSGADDAIITGSLPSGRVLTAADSGRMARAVIKAEQTVAISAEL
                     NARIVKMPFKEGDRFRAGAVLVEFDCQRIKAELAAAAAANKLHRNAVETVSELHRLGS
                     AGRFNLRQAQFEMEKSAAEVDNLKAKQATCTIRAPFDGSIAERLAAAHEVVSPNQPLL
                     RIVDKGEPEVQMIVPSAWLAWLVTGSVLDVEVDETRRRYPAKVQHISGAVDPVSQTVR
                     VIARLDGQPADVVPGMSGTARFGSAQPAAPADRVSGGKR"
     gene            complement(25982..27532)
                     /locus_tag="PLQ11_00400"
     CDS             complement(25982..27532)
                     /locus_tag="PLQ11_00400"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRK23390.1"
                     /translation="MQGQVYRSVRMAALAAALGLAGCSLRPSPLGAGDAAHYVADIRT
                     AIAFDHEPLARPLDLYEALARALKFNQELKAEAMSRLLGEADLALRSAEMLPQLVASA
                     QVYQRSNSAASVSRAVASGTRSGFYSESSERLSRSGDLSFSWNLLDFGVSYFRARQAA
                     DRSLISAEQHRKVAARIIEETRAVFWRAAALQKLDQGLRALDHEMTGALANAAQLSLA
                     GLTEPGEALGVQRDLLAIRRDIDLHRRSLVGAEDQLRALINLPATEPLRLSIEGQAPI
                     PMPGRLSFEDLAHVALTNRPDVRQSIYEQRITAEEARLAFLELFPGLNLVAGAALDQN
                     PFLLNQGWTSVAARASWQLVKLLQYPARSGLIEAKGELDRQRSRALAVAAILQAQVGV
                     LRLRHAHDEYRTLDRLANVQRQLARHTENQQRVGRVGTQATTRERMNALLSDSRRDAA
                     YGEVQAAYAAVLTAHGQDAAELGQAAIMPVADLAAWLRQAERSAFAVMPRPATGPERL
                     AATGNGGR"
     gene            27711..28448
                     /locus_tag="PLQ11_00405"
     CDS             27711..28448
                     /locus_tag="PLQ11_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HRK23391.1"
                     /translation="MRAPLLTLALLAGFSLPPAPALAGSCERTNLQQRKVVLCTMDLD
                     RQTVRTYWKAPSGEAYGSLSDIGRKAQPQPGRMIFAMNAGMYHSDLSPVGLYIENGAV
                     LKGPNLARGPGNFHMLPNGVFYVARRRAGVLEARAFVRAGLRPDYASQSGPMLVIGGR
                     LHPRINGGGTSAKIRNGVGVRGSMAYFIVTDEPVTFTEFARMFRDDLKAPNALYLDGS
                     ISSIYAPSVGRADSFMPVGPIIAGFDR"
     gene            complement(28624..29739)
                     /gene="wecB"
                     /locus_tag="PLQ11_00410"
     CDS             complement(28624..29739)
                     /gene="wecB"
                     /locus_tag="PLQ11_00410"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HRK23392.1"
                     /translation="MAEAGLKKIVCLVGTRPEGIKMAPVIRALKRQPWATTVVVSTGQ
                     HREMIRQTLGIFDIGIDIDLDVMEANQTLAGLSARIFQRLDPLLVDLAPDLVLVQGDT
                     TSVMVAALNCFYRRVPVGHVEAGLRTHDLANPFPEEMNRLIAGHASAIHFAPTEASYN
                     NLIGERHDAATVHVTGNTVIDALLDVAGRDLPCDFPRPGRRLVLITAHRRENFGAPLV
                     NICRAIQSLHDRFEDVDFVYPVHPNPNIRGPVFEHLSGLERLHLTEPVNYLDLTALMK
                     ASTFVLTDSGGIQEEAPALGKPVLVLRAETERPEAVNAGVARLAGTETQAIVEECSRL
                     LTDPAAYAAMARGISPYGDGKAAGRIVSLCRTFLKVV"
     gene            30066..31124
                     /gene="rfbB"
                     /locus_tag="PLQ11_00415"
     CDS             30066..31124
                     /gene="rfbB"
                     /locus_tag="PLQ11_00415"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010974014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HRK23393.1"
                     /translation="MRFLVTGGAGFIGSAVCRRLVRGGAEVLNLDKLTYAGNLTSLAS
                     ISNAGNYRFGRIDICDLGTVNEAFETFRPDYVLHLAAESHVDRSIVGARAFIDTNIVG
                     TFSMLEAARRYWSALPAAQKDRFRFLHVSTDEVYGSLGDTGLFTETTPYDPSSPYSAS
                     KAAADHLVSAWVRTYGFPAVISNCSNNYGPFHFPEKLIPLIILNALHGKPLPVYGAGD
                     NIRDWLYVEDHARALDMIVRNGRVGEKYNVGGRNERRNIEVVRAICNILDRLTPGGAP
                     HDRLITFVTDRPGHDHRYAIDAEKLEKELGWQAMETFDTGLEKTVHWYLENEWWWRPL
                     RDKVYAGERLGLTSGSPA"
     gene            31124..32011
                     /gene="rfbD"
                     /locus_tag="PLQ11_00420"
     CDS             31124..32011
                     /gene="rfbD"
                     /locus_tag="PLQ11_00420"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012493933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HRK23394.1"
                     /translation="MRLIVTGRDGQVARALMAEAPALGVILIPLGRPELDLLHPTGLD
                     ALFAAHRPDGIINAAAYTAVDQAENDYDAALAVNGAGAGAVATAAAALNIPIIQLSTD
                     YVFDGTKPTPYIENDPVAPINAYGRSKLAGERSVIDATPNHAILRTSWVYAATGKNFV
                     LTMLRLAADRSSVTVVSDQRGAPSYAPDIARAVLDVARNLATRPDDALLRGIFHMTGG
                     GETSWAEFATEIFRQSAARGGPSAAVTPVPTSAFPTPARRPANSRLDCGRLKTVHGVA
                     LPDWRDGLGRCMDTIWAHR"
     gene            32051..32923
                     /gene="rfbA"
                     /locus_tag="PLQ11_00425"
     CDS             32051..32923
                     /gene="rfbA"
                     /locus_tag="PLQ11_00425"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018328206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HRK23395.1"
                     /translation="MKGIILAGGSGTRLHPITLAVSKQLAPVYDKPMIYYPLSVLMLA
                     GIRDVLIISTPHDLPMFRKLLGTGKAWGMAFSYAEQPVPNGLAQAFVIGEDFVGDDRA
                     ALVLGDNIFYGAGLSNLLVEASARESGATVFAYHVPDPERYGVIDFDATGRALSIEEK
                     PKLPRSNWAVTGLYFYDRDVVEIAKKIRPSARGEYEITDVNRVYLERGELNVQRMGRG
                     FAWLDTGTPDSLTDASNFVRAIESRQGLRICCPEEIAFDQGFIDARQLRRLARALGKS
                     SYGKYLEQVLADAG"
     gene            32963..33511
                     /gene="rfbC"
                     /locus_tag="PLQ11_00430"
     CDS             32963..33511
                     /gene="rfbC"
                     /locus_tag="PLQ11_00430"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HRK23396.1"
                     /translation="MKFERFDIDGPVLVTPVRIGDSRGFFSESFRADKFEAEIGPTRF
                     VQDNQSLSVVRGTIRGLHYQKAPHAQGKLIRVLRGAVFDVAVDIRKQSKTFGRHVAVE
                     LSSDNGQQLWVPPGFLHGFCTLCDNVEVMYKVTDYYSPACDAAVRWNDPDIGIRWPIA
                     EAEAQLSAKDQTAPWLRDISPM"
     gene            33793..34509
                     /locus_tag="PLQ11_00435"
     CDS             33793..34509
                     /locus_tag="PLQ11_00435"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HRK23397.1"
                     /translation="MTISSLLKDRRLWLALAAIATIALVRWSGVGDLLALETLRAHRE
                     TLAAWVSAHVVIASLAYLATYVVVVAFSVPGAFVLTLWGGFLFGSLLGTALTVIGATI
                     GATAIFIFARTIFGENALDRFGVSAARLADGLRRDAASYLLVLRLVPLFPFFLVNLVP
                     AFVGVPLSTYAITTVFGIIPGTAVFSLAGAGIGSILDQGGTLTPGSILTPEIVAGLVG
                     LAILSLIAIPLRRHFGSGGG"
     gene            complement(34604..35479)
                     /locus_tag="PLQ11_00440"
     CDS             complement(34604..35479)
                     /locus_tag="PLQ11_00440"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003597352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HRK23398.1"
                     /translation="MLLMKAKLGVIGGSGLYDLPGLNDPYQQLIETPWGQPSAALRFG
                     RMGGKDIVFLPRHGDRHRLAPGGINYRANIDAMKRVGVTDIVSFSAVGSYKEDLPPGR
                     FILPDQLLDRTFARQTSFFGNGCVAHVAFGDPFGPRLIQRVAEAARRAGIDIRRGGTA
                     VCMEGPQFSTRAESLYYKGLGYDIIGMTSMPEAKLAREAEITYCMCAMVTDFDCWHSD
                     HADVSVDMVIRQLNQNMLAARRLLVELVADFPEEREDCPVGSHRALDHAIITPPEARD
                     PDMLAKLDAVAGRVL"
     gene            35948..37228
                     /locus_tag="PLQ11_00445"
     CDS             35948..37228
                     /locus_tag="PLQ11_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HRK23399.1"
                     /translation="MPKKAKELTAKAVASLKADGRYAVGGADGLHLRIAGASRAWVLR
                     LVVGTRVAANGRPAIHRRDIGLGSCDDVTLAQAREKARELRRQVRDGTDPLEAKEAEQ
                     RAKLAAKAEEERRKTFEECAEAVIEKKGEELRNAKALAQWRSTLEKYAYPVLNDGRKI
                     DELKKEDVLRVLKPIWSQKTETASRLRGRIETVFDHARAKDAYHGDNPAAWKGMLEPL
                     LPKPNKVKKVRHHAALPYAEIGAFMVDLRKREGMSARALEFSILTAARSSEARFATWG
                     EIDMDAKVWILPRERMKADKEHRVALCDEAIALLRALPRIEGDDIVFPAPRGGALSDQ
                     AFQKLFERMGWENITQHGFRSTFRDWAGETTAHPREVIEQALAHSLKDKAEAAYARGD
                     LMAKRQRLMADWGRYCGIVRGGESNNVVSMREAV"
BASE COUNT         7272 a        11786 c        11455 g         7116 t
ORIGIN      
        1 ctgcccgagc agatcgccca gcccgccggc gccggcctgt tgcgcgccct gcggctgcat
       61 ggcgccctga agcagggcat cgagaatctt cttggaatcg aacatgggta atggccctcc
      121 gtgccggccg cgcaaatgca ctgttaaact tgaacagaat atgaccagtt tgagcctatg
      181 caggtcaact gctggtttcg agttcaagaa tgattgtcga gagcggcggc agcgtcacgc
      241 gcgcctgcgc cgggcggcca tgcagcggtt ttgcaacggc cttgaccgcg ccgagattgc
      301 cgaggccgga tccgccatac agcgctgcat cggtattgat gcgctcgatc caccgcccgg
      361 ccttcggcag cgccagcaca tatccgtcgc gcggcaccgg ggtaaaattg ctgattaccg
      421 cgaccatgcc gccggttccg tcgcgccgca accaggcggt gaccgagcgg gcggcatcat
      481 cgaccaccag ccattcgaac ccctccggct cgcagtcgcg cgcgtgcaac gccggcgtgg
      541 cggaataaag ccgattgaga tcacgcacca gcgcgcgcaa gccgtcatgc ggggcgtgct
      601 gcatcagctc ccagtcgaga ccgcgttcct cgctccattc gcgccgctgg ccgaattcct
      661 gccccatgaa cagcagcttc ttgcccggat agccccacat gaagccgtaa taggctctga
      721 gatgggcaaa tttctgccag tcgtcaccgg ccatcttgtg cagcagcgag gctttgccat
      781 gcaccacctc gtcatggctc agcggcaaca cgaaattctc gctgaaagcg tagatgaggc
      841 cgaaggtcag atcactgtgg tggtgtttgc gatgcagcgg atcacgcttc agataggcga
      901 gcgtgtcatg catgaacccc atgttccatt tgaagccgaa gccgaggccg cccagatgca
      961 ccggctgcga tacgccggag aatgaggtgg attcctcagc gatcatcagc acaccgggat
     1021 gctgggcatg gacgatggtg ttgagttgcc tgaggaaagc aatcgcctcg agattctcgc
     1081 gcccgccctc gcggttgggg atccattctc cggccttgcg cgaataatcg aggtagagca
     1141 tcgaggcgac cgcatcgacc ctcagcccgt cgatgtggta gcgttccagc cagaacagcg
     1201 cattgttgat cagaaagctg gcgacctcct tgcggccgaa attgtagatg gcggtattcc
     1261 agtccgggtg gaacccctgt ctggggtcgg catgttcata gagagccgtg ccgtcgaacc
     1321 agccaagccc gtgcgcatcg gtcgggaaat gcgccggcac ccagtcgagg atgacgccga
     1381 ggccggcggc atgcgcccgg tcgacaaacc tggcgaatgc ctcgggcggg ccgaaccgtg
     1441 cggtcggcgc gaacaacccc gtcggctgat agccccatga cgcatcgagc ggatgctcgg
     1501 acaagggcag aaactcgata tgcgtgaacc ccatgtcgga gacatagggg atcagtgtat
     1561 ccgccagcat gtcatagctg ggaaagccac catcctcccc ccgccgccac gatcccgcat
     1621 gcacttcgta gaccgacacc ggcacgcgcc gttgatcgag gccttcgcgg cgggcacgat
     1681 actcctgatc ggtccaggtg aaaggctgcg gagcggtgat gatcgatgca ttggcgggcc
     1741 gcttctcgcc ggcaaaggcg taaggatcgc tcttgagccg caaatgtcca tccgcaccga
     1801 tgatttcgaa cttgtagggc gcgccggcgg caacatccgg caggaagatt tcccataatc
     1861 cggaagcgat gcgccgccgc atcagatggc gccgcccgtc ccacaggttg aagtcgccga
     1921 cgaccgagac ccgccgcgca ttgggcgccc agacggcaaa atgcgtgccc gtcaccccct
     1981 ggtgcatcag cacatgggcg ccaagccggt cgaacagcct cagatgcgtc ccctcggcgg
     2041 cgtaataatc atcgagcggg ccgaggatcg gcccgaaact gtagggatcg acaaatgccc
     2101 attcgccggc ggctccggtt gccaccagcc gcaccggctg gtccttgccc gggctggcaa
     2161 tgcgcccctc gaaaaagccg tccgcatggt ggcgttcgag aacgccgagg tcttcgccgg
     2221 acagggtggt cgcccgcaat gtttcagcgc cgggggtgaa ggcccgctgc accgtgccac
     2281 ggccatgccg atgcggcccc agcaccgcga aggggtcatc atgcatgccc tgcacaatcc
     2341 gggcgatggt caatctgtcg agccgttcgc tctgcaccgg actgtatctc cgcaggcggg
     2401 gaaccgggtt gctcgcgccg gcaccgccaa aaggccgatg aaacccggat gcttcggtgc
     2461 cggaggctgg tttttccctg ctgaatcact ataggctggc tcttgtgtga actgcaatgc
     2521 aaccacgacc tgcgagcgag aacctgagtg tcttttccca agccatcctc agcccttgtc
     2581 cccaatacgg cagctttcga aagcctgccg ctggtcaagc caacaggctt tcgcgaatat
     2641 gacgcgcgct ggtggttcgg gcatcaggga tcgcccaagg cgcctgaact caacctgatg
     2701 ggtgttcagg ctgtggggct tggaatcggc actctggcgc ggcgacgcgg cgtggcgatg
     2761 gagatcgtca ccggccatga ttttcgctcc tattcggggt cgatcaagac ggcgctgatc
     2821 gccgggttga tggcctcggg catgcgtgtg catgatatcg ggctggcgct ttcgccgatg
     2881 gcctatttcg cccaattcgc ccttgatgtg gcctgcgtcg ccatggtcac cgcctctcat
     2941 aacgacaatg gctggaccgg ggtgaagatg ggcatcgaac gcccggtcac cttcgggcct
     3001 gaagaaatgg ccgaattgcg cgatatcgtg ctgggcggac atttccacct gtcgggcggc
     3061 ggttcctatg tctttcatca gaactttgcc gaacgctata tcggtgacct gaccgagcgg
     3121 ccgaagcttc gtcgcaagct caaggtgatc gccgcctgcg gcaacggcac cgccggtgcc
     3181 ttcgccccgc aaatccttga gcggatcggc tgcgaggtga tcccgctcga ttgcacgctc
     3241 gaccattcct ttccgcgcta caaccccaac cccgaggata tgcacatgct gcatgccatc
     3301 gccgaggcgg tgcgcacgca tggcgccgat gtcggccttg gctttgatgg cgacggcgac
     3361 cgctgtggcg tggtggacaa cacgggcaac gagatttttg ctgacaagat cggtgtgatg
     3421 ctggcgcgcg acctttcggc ggttcatggt ccgtcgcaat ttgttgtcga tgtcaaatcg
     3481 accggactct atcattccga cccgcgcctg caggcgatgg gcgcccgcac cgactatttc
     3541 aagaccggcc attcctacat caagcgccgg gtgcgcgacc tgaaggcgct ggcaggattc
     3601 gagaaatccg gtcatttctt cttcaatgcg ccgatcggcc ggggctatga cgacgggctc
     3661 gtcactgcca ttgcagtggt tgacatgctc gaccgcaatc cggatcgctc gatggccgag
     3721 ctttacgccg atttgccgaa gacctggggc acgccgacga tgtcgccgca ttgcgacgac
     3781 gagatcaagt atcaggttgt ggatcgggtg atcgcccggg tgaaagcaat gcaggcagcg
     3841 ggcgagacca ttgccggcgc acgcatcacc gacatcaatc tggtcaatgg catccgcatc
     3901 ggcaccgagg atggcacctg gggcctggtg cgcgcctcgt ccaacaagcc agagcttgtc
     3961 gttgtggttg aaagcccggt ctcggaagcg cggatgcgcg ccatgtttgc cgctgtcgat
     4021 tccgtgttac ggcaaaatca agaagttggt gcctacaacc agtcgatctg agacaggtgc
     4081 ggcatctgcc tgatgcgcac caacgacgag ggcatcgatg acaaagcaga ttgttccggt
     4141 catcctctgc ggcggagccg ggacccggtt gtggcccgcc tcgcgcgaca gctaccccaa
     4201 gcagttcctt accttgaact cgaggcgatc gctgtttcag gacaccctcg cccgtgtcgc
     4261 cggcgccggc tatggccggc cgattgtgct gtcaggcaat gcctaccgtt tcctcgtcgc
     4321 cgatcaggcg caggcgctgg gcatcgcggc ggacatcgtg ctggaaccgg cgcggcgcga
     4381 ctcctgtgcc gcgattgcgg cagcggcggc agtggcggtc tcgcgcgatc ccgaggctgt
     4441 ggttctggtg ctgtctgccg accatgacgt gccggatcag gctgcatttc aggcccatgt
     4501 cgcgcgcggc ctggccggtg ctctgctcgg ccgcattgtc accttcggga tccagccgac
     4561 gttcccggcc accggctacg gatacatccg gcccggaccg gccctgcccg tgcccggcat
     4621 ccatgccatc gccgcctttg tcgaaaagcc cgatctggca accgccgaac gttatcagaa
     4681 ggaaggctat ctgtggaatt ccggcaattt cctgttcagg gcccaggtgt ttctcgacga
     4741 gttgaaggcc ctggcgcccg atattcatga tccggttgtc gaatccgtcg ctgcagccag
     4801 ccgcgacctt gatttcctcc ggctgtcgga gccggccttc acccgcgcca gggcgacatc
     4861 ggtcgattac gcggtgatgg aaaaaaccgc cctcagcgcg gtggtgccgt ccgatttccg
     4921 ctggtcggat atcggctcct gggctgcggt tgccgacatg gccgagaagg atgcggccgg
     4981 caattccgcc cacggcgatg ccatgttcga aaatgcccgc aattgctaca tctattcgcc
     5041 tgacaccctc acttcggttg tcgggctcga caatgtcgtc gtggtcgcca cgcgcgatgc
     5101 cattctggtg accgacagat ccaatgccga ggatgtcaag aagctggtcg gtcgcctctc
     5161 aagtgccggg cgtcgcgagg ctgtcgagca tctcaagaac taccgcccct ggggccatta
     5221 cgaacagatt gatcgcggcg cacgctatca ggtcaagcgg attaccgtgg cgcccggggc
     5281 aaagctctcg ctgcaaagtc atttccaccg ggccgaacat tgggtggtgg tgacaggaac
     5341 tgcgcgcgtg acggtggacg acaaggttca tctgcttggt gaaaaccagt cgatctatat
     5401 cccgctgggc gccgtgcacc gcatggaaaa ccctggccag attccgcttg aactgatcga
     5461 ggtgcagtcg ggcagctacc tcggcgagga tgatattgtc cgctacgagg atgactataa
     5521 ccgctcgtag atccgcctga ttgctcccac agtctccgcg tctggtcgca aggtcagttc
     5581 ttttgatgcg tgttcggcat ctgttggccg cagccggggc agaaccgcgc cgctaatgag
     5641 acatccctgg cgcagaccgg gcaacggcgc gcctcgccgg ggctcgcagc ctcctgcttt
     5701 gggctcaacc caaccatccg cgccatgtcc ttcagatctt ccttggtcgt caggtcgcgc
     5761 ttggcattgt aaacatcgtc caacaggaac tcgacaggcc gatcccgccc ggaccgatag
     5821 acgcccgctt tccaacccca gcgcaggatg ccaagcttgg ccagaagatc gagcaaacgc
     5881 atgcgccccc cctaaggcat caaccggtcg aattggaccg tcgccctgtc ccatcatcgc
     5941 tgccagactc ctgaatgcaa tgcgcaggat caatggcaga accttgcgca ccggacacgg
     6001 gcaagtgaca tgacagccag ccggccgcgc gtctttttgc cgatttacct gcgcctcgcg
     6061 atctcctaga gcacgcatgg ttttgattga tgcatcaaaa cacggacaac gtgatcgcca
     6121 tcaatggctt agagcatgct tctggcgatt aaccgactcc acctaatcgc agcatgctcc
     6181 agagcatgct gcctaaagtg ccagtgggca ggatgacatg ccggaaataa cctttgtcgc
     6241 cgccgatgcg acgtatcaga ccgtcaaggg caggagcggc gagacgctga tggaaacagc
     6301 gttgcgtcac gccattcccg gcattgatgc cgaatgcggc ggcgcctgcc gctgtgcgac
     6361 ctgccatgtc tttgtcgatg aggactggat tgaccggctg ccgcctcgca ccgatgagga
     6421 ggaggacatg ctcgataccg tctcgtcggt cagaaagaca tcgcgccttt cctgtcagat
     6481 ccgcgtgacc gcagaactgg atggtctcat cgtgaccaca ccgcgccggc aggggtgaga
     6541 gctagagcat gctgcgaaaa agtgtaggcg gtttttcgct taaagcatgc tctaaacact
     6601 tgataacgat cacgttgtct cacgtttcgg tgattcaacc gaaacgaggg cgtgatctga
     6661 cgtccctgcc ctgtgccagg tcggcctcag aatcggatcc cgtcaccgaa aggatcggcc
     6721 ggatcgcgca gcagaatggc ctcgccagtg atataggcgc gtccggtgat ggagggaatg
     6781 atggtgcctc cgctgccagg ctggtaactc gcctcgaagc gcgaaccgat cacactttcc
     6841 tgcacccatg tcgtgcccgg cgcgagtttg tcatcggcgg caaggcaggc gagcttggcg
     6901 ctggtgccgg tgccgcaggg cgagcggtca taggctccgc ccggacacag cacgaacgag
     6961 cgggaatggg cgccgggggc gccgggcgga ccgaacatct cgacatgatc gatgcgcccg
     7021 ccatcagcac cacgaatgcc ctgcgcatca agcgcctgct ggatggcggt ggcggctgcc
     7081 gtcaattcgc cgagatgagc cgctttgatc gggcagggaa cggtctcggt gaggaagaac
     7141 cagttgccgc cccaggcaac atcgccgaca acgcggccaa gccccgggac cttgacagca
     7201 atgcccttgg catgccggta gcttgccaca ttggtgatgc gcacctgatg agcgtcaatc
     7261 agctcaaacg ccacctcgcc aaccggggtt tcaatccggt ggcggcccgg cccgatacga
     7321 ccgagatggg ccagcgtcac cgccagcccg atggtgccat gaccgcacat gccgatcatg
     7381 ccgacattgt tgaaaaagat gatgcccgcc gcagcggtcg gatcgaccgg ttcgcagatg
     7441 agccctccga ccagagcatc ataaccgcgc ggctcgttga cgacgaaaga gcggatgtga
     7501 tcgaagtcac gctggaaacg tgccctgcgc tcggccatgg tgccgcgtcc aagatcaggt
     7561 ccgcccgcaa cgatcaggcg tgtcggctca cctgcggtat gggagtcgat caccttcatt
     7621 gcatccccca tggacccgtg actgattgtc cggttggaca tgtcgatcag tcctatccgc
     7681 gccaactggt cagttcaacc accagattgc agcgttgagc aaactcgcaa atcccaccca
     7741 cgcggcatag ggaatctgcg cctgcgccgc cgtccgatcg actgcggcga tcacgacgat
     7801 catccacagg atggttagtt caagcgccag gatgacgaac aggcccgcga gcgggttgcg
     7861 ggcggcaaag aacgcccagg accataacac attgagccca agctgcaccg caaggacggc
     7921 tagcgcctta tcacgccctg gtgttgccgc tggccatcgc agcagccgcc agaggctgaa
     7981 ggccatcagc ccgtataggc cggtccagac gatcggaaag acgatgttgg gtggcgtgaa
     8041 ggacggtttg gccagacccg gataccagtc aaccagcccc cgcatcgtgc caagctgccc
     8101 gagccaggac accgccacca caatcaccag cgacagccca agcttgatca gactggcaaa
     8161 ggagccggcc ggctcgcggc tgccaggctg cttcggcggc ggaaagcccg gctcgggatc
     8221 atcgggcaga cgcagggagg gttcggtcat ggacttgcct ttcacggtcg cgtggcaaga
     8281 tagacaccga ttgccgtcac caccatgccg gcaatctggc ccgttgccag cgtttcgccg
     8341 aacatggccc aggcctgcac ggcggctgtc ggcggaatga gatagatcag cgaggccact
     8401 ttcgacaccg cccctcgccg gatcatcatc agcatcagcg aaatcgcccc gatcgacaac
     8461 accagcacgg accaaaccag cccatagagg acctcgcgtg tgaaaggcag gttgagcggc
     8521 tccagataga gggcgaccgg cagcgtcacc atgagcgcgc cgagatactg caccgccgtc
     8581 aggctgcgca gatctcctgc cgcaacaaac cgcttctggt agaaggtgcc gatcgtgacc
     8641 gccaccatcg acacgatatt gaccaggatg gggcccgaca tcgcggcaac cgtctcggat
     8701 gtgacactgg acacccgcgg cgccaggacg atgccaaccc cgacaagccc gatcaccacg
     8761 ccggcgagat tggccagact cacccgctca tccagcacaa accgcgcaat catgatggtc
     8821 agcagcggtt gcagcgcggc aatcagtgcc gacagacctg ccggcagccc ggtgccgatg
     8881 gcccaccaga cgccaccaag atagatcgca tgaaggagaa tgccggaaac cagcccatgc
     8941 acccagtcca gtcgacggcg tggccaatct gctcccacag caaacgcgaa caggccgatg
     9001 acgatggcgg caatcgcata gcggaccacg agaaaggtga gtggatctgc tccggcaacg
     9061 gccgcgtatt tggcaacgat ccagcccgtc gaccagatca atacgaacaa ggccggagcg
     9121 acgcgttcta gcaccggggg cgccctctca ggatggaggc ccgtcgtaaa cgagaccgct
     9181 ccgcgtgcca agccgaaaca gacagccctt cctcgtgccg ggccggcaca ttttcccgtc
     9241 gctcgaccct ggcataggcc ttgcaaccgc gcccctaccc tgcggatgtg caggctagat
     9301 cacgccgcat tttgatggaa tcatcaagat gcggataacg tgatcggtat caataagtta
     9361 gagcatgtct tctgcgatta accggtatcc acttaatcgc gacatgctct aaagttggag
     9421 gttcagatgg ctgggtccct catcaagagc atgggcattg atcttctgtc gccgcaaagc
     9481 ctcatcgtga tcggctttgc cctggcggtg ttctgcacca gctgcggctg gctggcccat
     9541 ttcatcgccc gcgagatcag ctttggcatg cttggcaatc cggtggtgat gttcgtcagc
     9601 ctttggcttg gcctgatcgc ctttcatgcc ctggccgggc cgctgccaca gcatgccccg
     9661 ctgaccattg cctatgtcgc ggttgcctcc gccatcggcg gattgctcat cgccagcacg
     9721 ctgaagggcc agccctggcg cacctgacga gcctgccgca cactcttccg gaaggatctc
     9781 cacgaggccg ggatcaggca gccgttgccc gtgctcctga ccaggcagga acacgggcca
     9841 atcgaaccat ggtcaattga gcgccgcatt cttcggtcga cgcgccggtg ctgccggctc
     9901 cgacccgacc atcagccccc ccgcgccgac gcggatgggc accgtctcgc catcacgcac
     9961 cgccccggca agcagctgcg ccgccagcgg atcctgcagc gctttctgga tcacccgctt
    10021 gagcgggcgc gcgccatagg ccggatcgta gccggcttca gccagccagg ctcttgctgc
    10081 ttcatcaagg tcgaggctga tcttgcgctc ggccagcagc ttgccgaggc gggccatctg
    10141 gatatcgacg atcgcgccca tatgctcccg ccgcagacgg tggaacagga tgatctcgtc
    10201 cacccggttg agaaattccg gccggaaatg gtggcgcacc actgccatca cctcatcgcg
    10261 caccgcatcc gtttcttcgc cctctttctg gttgacgaga aactccgatc cgagattgga
    10321 ggtcatgatg atcagcgtgt tgcgaaaatc gaccgtgcgg ccctggccat cggtcagccg
    10381 gccgtcatcg agcacctgca acaacacatt gaacacatcg ggatgggcct tttcgatctc
    10441 gtcgaacagc accacctgat acggccgccg ccgcaccgct tcggtcagcg cgccgccttc
    10501 ctcgtagccg acatagcctg gaggcgcgcc aatgagccgg gcaaccgaat gcttttccat
    10561 gtattctgac atgtcgagac gaaccatcgc ggtatcgtcg tcaaacagaa aagcggcgag
    10621 cgccttggtc agttctgtct tgccaacgcc ggtcgggccg aggaacatga acgagccgat
    10681 cggccggttg ggatcttgca aacccgcccg cgcacgccgc accgcggtcg aaaccgccgc
    10741 aagcgcttcc tcctgaccga cgacccgctt gccgatctct acttccatcc tgagcagctt
    10801 gtccttttct ccctcaagca tccgatcgac cggcacgccg gtccagcgcg acacgacagc
    10861 ggcaatgtga tccgacgtca ccgcttcctc aagcatgccg ccgcccctgg cctcgatctc
    10921 ggccagctgc ttttcgaggc cggggatgac gccataggcg agttcaccgg cccgctggta
    10981 ttcgcccttg cgctgggcga tggcgaggtc attgcgcgcc tgatcgagcg atttcttcag
    11041 atcagccgcc ttgccgagct tggacttctc cgcctgccag gccgtggtca gcgccgcaga
    11101 cttttcctca agctctgcca gttccttttc cagccgctgc aaccggtcct tggaggcggt
    11161 gtcggattcc ttcttcagcg cttcctgctc gattttcagc cggacgatct cgcgatcgag
    11221 actgtcgagg tcctctggct tggaatcgac ctgcatgcgt aaccgcgcgg aggcttcatc
    11281 gacgaggtcg atggccttgt ccggcaggaa ccgatcggtg atgtagcggt tggagaggct
    11341 cgccgccgcc accagtgccg aatcggtgat gcggacaccg tgatgctgct cgtatttctc
    11401 cttcagcccg cgcaggatcg agacggtatc ctccaccgtc ggctcggaga cgaacaccgg
    11461 ctggaaacgg cgggcgagcg ccgcgtcctt ttcgacatgt tttctgtatt catcgagcgt
    11521 agtggcgccg acgcaatgga gttcgccgcg cgccagagcc ggcttgagca ggttcgatgc
    11581 atccatcgcc ccgtccgcct tgcccgcgcc aaccagcgtg tgcatctcgt caatgaacag
    11641 gatgatgctg ccttcggcgg cggtgacctc gttgagcacg cccttcagcc gttcctcaaa
    11701 ctcgccgcga tatttggccc cggcgatcag ggcgcccatg tcgagcgcca gcagggcctt
    11761 gtcggcaagg ctttcgggaa catcaccgtt gacgatgcgc agcgccagcc cctcgacaat
    11821 cgctgtcttg ccaacgcccg gttcgccgat caacaccgga ttgttcttgg tgcggcgtga
    11881 cagcacctgg atcgtccggc ggatttcctc gtcgcgcccg atcaccggat cgagcttgcc
    11941 ggtgcgcgct gcctcggtca gatcgcgcgc atatttcctc agtgcgtcat actggttttc
    12001 cgctgtggcc gaatcggccg tgcgcccctt gcggatcgcc tcgatcgcgc cattgagcgc
    12061 ctgcggcgtc acgccgccgc gctgcagggc ttttccggct tcggtgtcct tctcgacggc
    12121 cagcgccagc aggatacgct cgaccgtaac gaaactgtcc ttggccttct ccgcagcggc
    12181 ttcggccgcc gccagcaccc ggcccagcgg cgccgtcaga tagatcgaac cggcgcccga
    12241 tccctgcacc ttcggcattt tggccagcgc cgcatcattg gctttgcgaa cctcttctgg
    12301 ctgcccgccg gcgcgctgga tcaagccatt ggccatgcct tgctcatcgt cgagcaaggc
    12361 tttcagcaga tgttcggggg taaagttctg gtgaccgtcc ctcagggcga tggcctggcc
    12421 cgcctgcagg aagccgcgcg cacgttcggt gaacctgtcc aaattcatgg tcatccctct
    12481 atcagcaagg atcgcgcccc cgatcaggca gcgctattgt gatgtgccgg acccgatcgg
    12541 catccggcgc ttgctgatgt ggggagggca atggcagaat caaggacccg cgcgtcccgc
    12601 aaggcacttt gaggttatcg tgttgatcca tgtcacccgc ctgactcaat atcctgtcaa
    12661 gggcttcacc ggtgcggacc tgccgcgggt gcaactggtt gccggggacc attttcccgg
    12721 cgaccggatg tttgccatcg agaacggccc ttccggcttt gatccggccg cacccgtcca
    12781 tcagcccaag ttcaaatttc tggtgctgat gcgcatgccg gctcttgccc ggctcactgg
    12841 ccgctatgat gcggcaacga accgcttcac ccttcttcag ggcgatagag aggtcgccgc
    12901 cggtgaattg acaacatccg aagggcgtaa aaccatcgag gattatctgg ccgcgaccct
    12961 cgcaggcgag cttcgtggtc cgccgcgcat cctgactgcg ccacccggtt tccgcttcat
    13021 ggactcacaa tcgggctata tctcgatcct gaacctcgcc agtctcaggg cgatcgccgc
    13081 cagggcaggg cgcacaattc ttgcgccaga gcgtttccgg gcgaacatca tcaccgacgg
    13141 tctgccaccc tggggcgagt tcgatctgat cggcaaaacc atccggatcg gtgacgtcac
    13201 cctgcgtatg atcaagcgaa ttgaccgttg cgccgccacc catgtcgatc cagtgcgcgg
    13261 catccgcgat cttgatctgg tcggcctgct tgaacgtgag ttcggccacc atgattgtgg
    13321 cgtctatgcc gaagtcgtaa ccggcggcct cgtcagcgtc ggagacagga tcctcgcatg
    13381 aaggaagccg cagccgtcac tcactgatgg ccggctgcgc atcctcgccc gcaggccctt
    13441 cgcctgaggc catatcctcg cgtggtcctc ggcgatgacg ccgccgccgc ccccgcacgg
    13501 ggaaccggcc gccttcatcg ccagactgtg cattgtcacc ggcctccgca aatccgcctg
    13561 gcgcaggacc atcaccggcc tcgatcggaa tgcggacggg cgcggtcaga aaagccggca
    13621 ggccgccctc gtcgtcggcg gcctcgggct gcggcctgtc tggccgcggc tggttgcgat
    13681 caaagccacg gtcgcggcga aagtcgccgt tgttgcggtc tcgccggaaa tcaccgcgat
    13741 gttcgcgcgg ctggtcttgc cggccgtgct ccatgcgggg ctgatccgga cgcggagcga
    13801 actcgtggcg tggctgctcg ccattgggat ccacatatcc cggcggaggc tggatctgcg
    13861 gagcgcggaa ggtgaagcga tccatcaccg agctttcgaa ctcgtcatcg ccatccatgc
    13921 cgtcatccgc ttcatcgaca cggccaccct gcatcaactg ctgggcctgc atctgctgct
    13981 gctgggcagc ggcgatgaga cgcagataat gctcggcgtg ctggagatag ctttcggccg
    14041 cgaccggatc gcccgaggca tgggcatcgc gggcgagcga tacatatttc tcggcgatat
    14101 gtgctgccgt gccgcgaatc ttgacgtcgg gcccgttgga ttcatacgaa cgggtcaatg
    14161 gattgggccc gcggcgattg cggccccgca tgcgcttgtt ctgtccaggt ctcatcaatg
    14221 gttctctggg gtcccagcca ggccggatcg ttcgccggcc gcttgcatct cttccatcgg
    14281 cgtcctgtca acgccgcgtc catttcccgg ccctgcctgg caaacagcag cctgacaggt
    14341 ctcgtttgaa cggtccagtc tgtcaggtct ggaatgtgcc accgggcgtg gaccactggg
    14401 cgcctgaatg acctgataac cggattgtcc cgagcctggt gcgaacccag cccgcccacc
    14461 gttcgagcga accgcatcca atcgactcat atcccgagca cattgtgcat agcgggctca
    14521 atgggtattg gcaagcgaaa gctcagcttt cttctgtgcg cccgagtgct gtcccccggc
    14581 gaccggcgca cccctcttgg agcgctcctg caatgctcgg aaggataaag taatcgtccg
    14641 ttcattactt caggttgcat tcgagcaaag tacattttta gaaaatcagc gccaaaactg
    14701 ctccgcctaa ccagctatta agaatctgta aaccatactg aaataccaag gacagaccgc
    14761 accgtcgcgt gcctgtctgc ctggtgatcc tgacatgaaa tcggctttcc tttttgccag
    14821 caccgctcta gccattgctg caatgcccgc cttggcatcc gcccaggggc tgcggctgac
    14881 acccgcgccc ggtgccgtga cgcccgtccc gcaccctgtg cccgcagcgg caagcggctg
    14941 gtatctgcgc ggcgatgtag ccgccaatgc caaccagatc ggttcgctga aacaggacaa
    15001 tctgacgctg aacggcggca gctttctatc aaagtccgcc tcgaacaccg cttcggtcgg
    15061 tgtcggcgtc ggctatcgtt tcaacgacac cctgcgtgtg gacgcaacct gggaactgcg
    15121 caccggctcg aatctgaagg ccgtcgacaa cgtccgcatt ctcaacagcc gggggcagac
    15181 tgccgcagac atctattccg tctatgacgg caacatcggc acacaggttg cgctgctcaa
    15241 cgcctatatc gatcttgcca actggaacgg gctgacgccg ttcatcggcg ccagtatcgg
    15301 cgttgcgcgc aactcgattt ccggcctgac gaccagcaat gcctccacgt tgaatatcta
    15361 ttccgctacc gctccctatg acctgaccag ccgcatcagc gacgcctcga gcggttattc
    15421 gccggaccgg gtcagatatt cttttgcctg gggactgacg gcaggtctgg cctatgcggt
    15481 gagcgaccgg ctgaccctcg agatggccta ccgctatctc aaccttggca atggcgctca
    15541 aaccagcctc attcattgca cctgcggcac catcggcacg ccgctcaagg ttggcaatct
    15601 gtcgagccac gatgccaagc tcggcatgcg ctggaccttc tcggacacgc cccgcgtgat
    15661 gtcccactat cagccggtgg tcgccaaata ttgatttgac cggaaatgat ggcctgcggc
    15721 gtcaatccgg ccgcagccgg cgggacggct tgatcggaac gcctttggtc aggaacgggc
    15781 gggacccggc cttcatgtcc ttcggaaact ggcccttgga cctgacacgc cggccctcgc
    15841 cggcagagcc cgtcccggct ggctgccagt tcggcacgag gtgatgcttg cccgggccga
    15901 tcagatcggc ccgacccatg gctttcagcg cttcgcgcag caccggccag ttttccggat
    15961 cgtgatagcg caaaaaggcc ttgtgcaaac gcctctgcct gagacctctc accgtttcga
    16021 ccggatcgga agcgccgcgg cgcaccggtt tcagcgggtt gaccccggta tgatacattg
    16081 ctgtcgacaa tgccatcggc gaaggcagga acgtctgcac ctgatcggca cggtagcgat
    16141 tcttcttcag ccacaacgca agatgcatca tgtcctcatc cgttgttccg ggatgggcgg
    16201 caatgaaata cggaatgagg aaatattgtt tgccggcttt cttggccgcc gcatcgaaca
    16261 gcgccttgaa tgcatcatag ctgccgatcc ccggtttcat catcttggag agcggcccct
    16321 cttcggtgtg ctcgggcgca atcttcagat agccgccgac atgatgttca accagctcct
    16381 tcacataggc ggggctttcg acagccaggt cataacgcac acccgatgcc accatcacct
    16441 tcttcacgcc cggcacggcg cgagccttgc gatagagctg gatgagggct tcatggctgg
    16501 tgttgagatt cttgcagata tcgggaaaga cacaggacgg ccttcggcag atcgcctcgg
    16561 tttccttgtc cttgcagccg atccggtaca tgttggcggt cggtccgccg atgtcggaga
    16621 tgattccggt aaacccttcg gtgcggtcgc ggatggtctc gatttctttc aggatggagc
    16681 cttcggagcg cgactggatg atccggccct catgttcggt gatggaacag aaggagcagc
    16741 cgccgaaaca gccgcgcatg atcgtcaccg aatggcggat catctcccag gcagggattt
    16801 tcttcccctg ataggcggga tgcggcgccc gcgcgtaggg aagttcataa actccatcca
    16861 tttcggcggt tgacagcggg atcggcggct cggtcagcca cagttcgcga ttgccatgcg
    16921 cctgcaccag cggcagggca ttgccggggt tgctttcctt gtgcaacacg cgcgatgccc
    16981 gggcataggc ctcacgatcg gtcgagacct gctcgaagga cggcagccgc agcaccgtcg
    17041 gctgatcctg ccgctccttg cgccgaacgc cctcgtccgg atcatcaaga tcgtcggcat
    17101 gtgcctcgca ccagccgtcc ggcacaccgg agcgcaccag agccagaccg cggatatcgg
    17161 aaaaatcctg ccggatgccg gcacgcgcaa tcctgtgggt gacctcgaca agggcccgtt
    17221 cggcattgcc ataaatcagc agatccgcct tggcatcgat cacgatcgag cggcgaacct
    17281 tgtccgacca atagtcgtaa tgggcgatgc gccgcagcga cgcctcgatc ccgcccagca
    17341 ccactggaat gtcgggataa gcctcacgac accgctgggc atagacgatc accgcccggt
    17401 ccggtcgccg cccgccctcg ccgcccgggg tatagctgtc attgtgacgc agccgccggt
    17461 cggaggtgta gcggttgacc atggaatcca tgttgccgcc ggttacaccg aaatagaggt
    17521 tgggctttcc cagcgccttg aatggatcag cgccctgcca gtccggctgg gcaatgatac
    17581 cgacccggaa tccctgggcc tcgagcagcc tgccgatgat cgccatgcca aaactcggat
    17641 ggtcgacata ggcatcgccg gtcaccagaa tgacgtcgca ggaatcccag ccgagcgcat
    17701 ccatttctgc ccgcgacatc ggcaggaagg gcgctgttcc aaaccgttgc gcccaatgct
    17761 tgcgatagcc aaaaagcggt ctggcttccg gcgcggccgt gcctggtgtt cccatggtgg
    17821 tgcccgtcct caaccaaacc gcaagtgcgg catgaaaccc gcgcgcaacc gcatcctgcc
    17881 gcatggaagg gctccatgcg gcaagttgcc gctgtcgtca atgccgcccg tgcacctgac
    17941 cggaccggtc ggcgtttagc cgagccacca cggcctgaaa gtggccccgt tgaggctgaa
    18001 actgggagtc gtggtcggag ggggcgttgt gatcgcgccg tcgccggcaa gcaggctcct
    18061 gagatggctg agatcgccga aggtgttgat gcaggtaacc gacacaggcg cgaccgctgg
    18121 cgctgcgacc gcgatcgagg cgatcgccgt tgccgtggcc ccattggcat cggttacccg
    18181 atacgtgaag ctgtcggtac caacgaacag gtcacgcgcc gtatagctga acgtgccgtt
    18241 gggattgaaa gtcagcgtgc cgccggtcgg ctgtgacacc agcgcgaaag tcagggcgtc
    18301 gccgtccgga tcggaatcgt tggtggcaac tgttccggtc accgttgtgg agccggtcgt
    18361 ggcgaagttg tcggcaacag ccaacggtcc cgggttggtc acgttccagg cgaaggattg
    18421 aacggcgctc aagccacggg gatcagtagc cgtcacggtg attgcataga cgccgttgcc
    18481 acccggcccg cccgcgcagg cattacggtc gatggtgccg ctgatcactc cggttgtcgg
    18541 gtcgatgcta aggccggtcg gcagcccggt gaccgtgtag gtcagggggt tgctgtccgg
    18601 atccgagaag taggacgaca tggtcaatgt ggccgtacca ttgtcgagat tgttctgcgt
    18661 tggcaccgaa ccaaccgcga ccggagccga gttcacctcg atgaccgtta tggtagcgac
    18721 ggcggaatcg ctggcgccct gcgagtcagt aaggcagtag gtgaagctgt cagtcccgac
    18781 gaacccggca ttgggagtgt aggtgaagct gccattggcg ttgaccacca cggttccgtt
    18841 ggcgggagcc gttgtggcgc agaaggtcag cgaatcgccg tcgggatcgc tgtcattcgg
    18901 cgccagagat cccgtcagaa ccgtgccgga attgaccgaa tacgcatcat tgacggcgtc
    18961 tggcgccgga tttgtcaccg tccaggtgaa ggtctgggtg gcgctgccgc cggagagatc
    19021 aagcaccgtc agagtcacgc tgtaaacgcc attgctggtc ggaccaacct gcgaggccaa
    19081 ccggtcaatc gtcccgctga ccatgccggt cagcggattg atcgaaagac ccggcggcaa
    19141 tccggtcgcc gaatagacca gaaccgggct gtcggcatcg ctcacctggg tggcgaggtt
    19201 cagggtgatc gaggcgccat ccacattggc ctggcttgcc atcggggcag cgatgaccgg
    19261 gttggtattg gtcgccagca cggtaacgac gactgtgccg gtgtcggtgc cgccgcgacc
    19321 atccgaagag gtataggtga acgtgtccgt gccggtgtag ccggtgttcg gcgtataggt
    19381 gaaggtgccg ttggaattga gcgttaccgt gccgttcgac ggaccggtag cgacagtgtg
    19441 ggtcagaata tcgccattcg gatcggaatc attggcacag acatcgccgg agaccgccgt
    19501 gttgacggag gtcgaatagc tgtcgttgac cgcattgggc gggctgttgg acggtccgct
    19561 gacggtaatg gtcacggtcg ccgtgtcggt gccgccattg ccgtcattcg acgtataggt
    19621 gaacgtatcc gtgccggtat agccggtgtt cggcgtgtag gtgaaggtgc cgttggaatt
    19681 gagcgttacc gtgccatgcg aggggttggt cgtcagcgaa tgcgagacga tatcgccatc
    19741 cggatcggaa tcattggcac agacatcgcc ggagaccgcc gtgttgacat tggtcgaata
    19801 gctgtcgttg accgcattgg gcgggctgtt ggacggtccg ctgacggtaa tggtcacggt
    19861 cgccgtgtcg gtgccgccat tgccgtcatt cgacgtatag gtgaacgtat ccgtgccggt
    19921 atagccggtg ttcggcgtgt aggtgaaggt gccgttggaa ttgagcgtta ccgtgccatg
    19981 cgaggggttg gtcgtcagcg aatgcgagac gatatcgcca tccggatcgg aatcattggc
    20041 acagacatcg ccggagaccg ccgtgttgac attggtcgaa tagctgtcgt tgaccgcatc
    20101 gggtggtgcg tttggcgcgg ccgcgacaac aatcgtgacg gaggcattgg cgaaattggg
    20161 attgctgctg gtattgtcag tgacgcggta gatgaagaaa tcctcaccgc cgaagccatt
    20221 gttcggcgta taggtgaagg tgccgttgga attgagcgtc accgagccat gcgccggctg
    20281 cgaatggacg ctgaaagtca acggatcgct ttcagggtcg gtgtcgttcg gactgacatc
    20341 gccggaaacc ggtgtgttat gcgccgtcgc ataggtttcg gacaccgcaa ccggcggagc
    20401 cagggtcgcg tcataggctg cgaacacaag cgcatccgtt tccaggctgc ccgacacata
    20461 ttcaagatcc cagtcgccgc caagccgggc ggcgcccgtc agatcatctg aaccggcgac
    20521 atccgcgccc gtcagggacg caagcaggcc ggctgccgcc tcgccgggcg tcccatcagc
    20581 aaaattacag ccatagacga ggatatcggc attttccgac agatgcgtgc ccatcccggt
    20641 cagcgtagag cgataaaggc cattcatcgt ctccgcgttc agcaccgacg caccaaggct
    20701 caactcacct tgcgtgccat gggcgaaaat atggaccgct gcgaccccgt cgcggctgct
    20761 cagcgcttcg gcaagctgga ccactccatc acgcgaagaa tcgatcatca gaatgtcggc
    20821 gcccgccgga aggccggcaa tcagcacgtc cttctgatcg acattggcgt cgatgacata
    20881 cagcgcaccg ccgcccgatt gggcagcaac ctcgttggcg acaagactgt ccgtcgtcgt
    20941 gctggtatgc tcgccaggat tctggcttgc ctggaccaga gaattgccca gcgacatccc
    21001 gccatcatcg gccagcccgc agcagccttc cggctgccca tgatcgatca acgcaccagt
    21061 aagggcggca tcgaacacgt accgccgctc caattggata agagcatgcg acagcgtgga
    21121 ttcatgggcg caagcagaag tcttgggcgg aagcgggcgg gctgcagact tgaagaaacg
    21181 catggaactg acctgaggac ttgctggcga cgcgcgggcc attctggcac cgcgccaaca
    21241 ggtcagatag agaagtatat agataaatct tttctaaaaa tacttgaaaa taaaaaactg
    21301 gatattcgct aattgagcca gaaaatatat aattaaccat cactgtagat gatgcgttaa
    21361 gatttactac tcccgatgga attggaaaag tcgctgctat gcaggatgtg cattcccgcc
    21421 gaagccggat cagaaaccgc cctcgcgttg cagcacctgt ccgatccgtc gtaagatcct
    21481 tgccgccggg ttttcccccg cagccgctat gcgaatggtt ccgcgatcca gcagcagcgg
    21541 tgccgcgcct tcgccctcaa gcgcgatagc gacaagggcc tggcgcggaa ccgcctttcg
    21601 ccgatcctcg gttacggcaa ttggaccgcc atgacgatcg atcaggaccg gttcgccgag
    21661 gcttgcctcc gtcgcctcgg caacggatgt caccttcaac gaaaccggcc gtctcagacc
    21721 tgtttcggat acaaagacag cgttcgcacc gacctgcagg cgacgcgcat cgatctcctc
    21781 ggtgagggcg taagcgcgca cgccccggtc ggcaacaatc cgtgccagct cctgggcagg
    21841 gctaacccag actcccagcg caagaccggg atcgagatcg accaccgttc cactggccgg
    21901 cgcccggatt tcgagatcct gcgccaggcg ccccagtgca gcccgcttct cgcgagcact
    21961 gcgcaattgc gtctccagca cgggcaacgc atcccggtcg ctatcaaggg ccggagcacg
    22021 cgacaggcgc aattcaagtg cctgaatctc cgcatcgatg cgacggacct gcgtcattaa
    22081 caacggcgac tccagccgcg ccagcatctg gccttccaca acgacatcgc catcggcaac
    22141 catgaggtcc gcgatgcggg cggggctgcg ggcatgcacc ggcaaatcgt tgcgtgccga
    22201 cagcacggca tgggcctcga caatccggtt ccagggcacg acggccagac agcagagcag
    22261 gcccagccca gcagcgaagg cacggatccg ttgcgtggag agtctttcgc gcatcgtcca
    22321 ccagtcggcg acttcacgca caacggggcg cacgacgaaa tagaggattt caaacaggaa
    22381 caggacgatc ccgagcagct tgaacgacaa tgcataaacc agcgcagcaa tgcccagata
    22441 gagcccgagc cggtaaatcc aggtgacata gccaaagaca accagtgcgc ggcgcaaagc
    22501 gtcgctcaga tcgggcgccg gcgcccttgg tgccccaagc agcaggtcgc gcaggtggtc
    22561 acgtgtcagc gcaaaggagc gatcctgcaa gttgggcaca ttgagcaggt ccgacaacgc
    22621 gaaatagcca tcaaaccgca tgcacggatt gaggttgatg agaaccgatg tcgtaacagc
    22681 catggtcgcg accgccaccg ccatcagccg catcgtcccc tctggcagga acggccacat
    22741 cagcagcgcc agaacggcaa ggatggcctc ggcgccgaca ccggccagca cgatggccag
    22801 ccgctgacgt cgccgtgtca gccgccagct gtcggtcgta tccgtataga gcacgggcac
    22861 accgagcatc agcgccagcc ccatggtcgg aacccggcag ccggcccgat gtgcgacaac
    22921 ggcatggccg aattcgtgca cgatcttcat cccgaacagg accagcgcat aaatcggcag
    22981 ggcatcgagc cggaacaact ggctgaggtc gccagtgaag gcagaccatt gccgggcaat
    23041 cagatagccg gccaccagca gcgccccggc acaggctacc agaaagccgc tggaaaacag
    23101 cggcgccaaa cgcggcgtca tccagccgag cagtgtctca ggattgaaaa gcggcagccg
    23161 caggaacagg aaggactgca ttgccgcgat cagcggatgg ctggcgcggg cctcgcgctg
    23221 agccgccagg cctcgccagg actgcgagcg atctgcctct gtcagctcat tgcgatagag
    23281 aaactcggtg acggcgccga tatcagccgc cgacaccgac aggccgagtt ggcgttcaag
    23341 gtccgtggca agctcggccg cagcgatcgg acgccagagc cggacgatgg ccgaggccgg
    23401 ccaggcgagc tgaaaatagg cattgcgcgc aggatcgacg atcagcaggc cgggctggcc
    23461 aaggcgatcg ggcgccgctt ctgaaaattc cagttcagcc cgcaatcgcg gcagcgcggt
    23521 cggcgggata ttagcctgcc ccgccaaggg aacggatacg gtggccgcat tcatcggcta
    23581 gatgcccagc ttctgccgca aggcagcgat cgggcggcgg aacagataga atccgagcgg
    23641 caccagcgat ccgtccagcc gcgccgtgcc gcgcgcgccg agccgtggcg ccgggcttgc
    23701 ctcgggaaag tcggcaaaga tgcgatagac catctgctgg tcaggcccgg gtgtcggccg
    23761 atagccaatg cgtgtcaggc ttgccgatct ggcgccgagc ggctggccat cgagaaacag
    23821 tcgcacgccc atgcccggct caagcgtgag cgaatcggca acaccgaggt caatgcgcaa
    23881 ttcgacatcg gacgggtcga caatgtccat cacccgctcg ccggtggtga tcggccggcc
    23941 gatccagtcg gttttggcgc cgaacaccgc gacaccatcg cgcggtgccc tgacctcgac
    24001 gcgctgcaac agatcgttgg ccagcgcaag ctctgcgtcc gccacatcga gatcggcccg
    24061 cgccacggcg atttcatggg aatcgcggtg attggccact gcactttgga tcacgcgctg
    24121 aaagcgggca acggcaacgg cccggttctt ctgggcgatc tcgacatcat tgcgcagctt
    24181 ggtatcgaca aatccgaaca gcttgtcgcc tttcttgacc ggatcacccg gactgaccgc
    24241 gatcatggcg acaatcccgt cgagcggcgc gctgatcagc gtaggctggc ttcccaccac
    24301 ctcggccggg gcaagaactg agaaccgcac agggaacagc aatactgcca gcgccaccat
    24361 gggcaccatc agcatcagcc tgccgctggc caccatgcgg cgcaatccgg ggcgcagacc
    24421 aattccgttg cccgccagcg cggcataggc atgagcgaag ggctcgccga gcgcggcaag
    24481 accgacgcgg cggtccgcag gccagagcgt agagcggaag gccgcgagcc cgccgatgaa
    24541 tcgcccatcg cccgtctgca aaggaaccca cagcagatga ggaaaggccc tggtgcgctc
    24601 agccagttcc gagttcggag cctgccgcgc aaggtcaaga ccctgtaccg ccttcgtgtc
    24661 acttggcgca gaatcggcga ctgtcgcgat gaattctgtc accgccctga ccagtggcgc
    24721 ctcgggatcg acactggcag tgcctgaaac cgccgtaaca cgccagctgc atccatcctt
    24781 gcgcgacagg ctgccatccg tcgccgttac gcattcaacc agcaaggcac aatcatagcc
    24841 ggctaggtgt ctggcgccgg tcgtcagcgc caagcgcaac gatgccaggt ccggcgcgcg
    24901 gcggaaatcc tgttccaggt cgatgaatgc ggcaacggcg ggtgatgcgg ccggttggac
    24961 cgcaacggca gtcgcaggcg ccttgattga ggatgctgcc ccatgcgatg gcgctcggac
    25021 gatacgacgc aaggcggtca tcgtttgcct ccgctgacac ggtctgccgg tgcagcaggc
    25081 tgggccgagc cgaagcgggc agtcccgctc atgccgggca ccacatcggc tggctgacca
    25141 tcgagccggg cgatcacgcg aaccgtctgg ctgaccggat cgaccgcccc cgagatatgc
    25201 tgcaccttgg cggggtagcg ccggcgcgtc tcgtcgacct cgacatcgag aacgctgccg
    25261 gtgaccagcc aggcaagcca tgccgatggc acgatcatct gcacttccgg ctcgcccttg
    25321 tcgacgatgc gcaacagcgg ctgattggga ctgaccacct catgggcggc cgccagcctt
    25381 tcggcgatgc tgccgtcaaa cggcgcccgg atggtacagg tcgcctgctt ggccttcaga
    25441 ttgtcgacct cggcggccga tttctccatt tcgaactgcg cctgcctcag gttgaaccgc
    25501 ccggccgacc cgaggcgatg cagctcgctg accgtttcaa cggcgttgcg atgcagcttg
    25561 ttggcggctg cagccgctgc aagttccgcc ttgatccgct gacagtcaaa ctcgaccaga
    25621 accgcacctg cgcggaaccg gtcaccctcc ttgaacggca tcttcacaat gcgggcattg
    25681 agttcggcgc tgatcgccac ggtctgttcg gccttgatga ccgcacgagc catccgtccg
    25741 gagtcggcgg ccgtcaggac acgcccggac ggcagggagc cggtgatgat cgcatcgtcg
    25801 gccccgctgg atgacttgac gggatcctgt ctggcgctct gcggcactgc cttgcgcgtc
    25861 tggacctggc gcaccggagg cggcggttca tccgtccaca aggccgggac gagctgggta
    25921 accaccagca gactggcgat ggtcagggcc gctgtcggcc acaggctggc gcttggctcg
    25981 ctcatcggcc accattgcca gtggcagcca accgttcagg cccggtggcc gggcgcggca
    26041 tgacagcaaa agccgaccgc tcggcctgcc gcagccaggc cgccagatca gcaaccggca
    26101 ttatcgccgc ctgaccgagt tcggcagcat cctggccatg cgcagtcagc acggcggcat
    26161 aggcagcctg tacctcgcca taggcggcat cacgccggga atcggacaaa agtgcattca
    26221 tccgctcccg cgtcgtcgcc tgggtgccga cccgtccgac gcgctgctgg ttctcggtgt
    26281 ggcgggccag ctggcgctgg acgttggcaa gccggtccag cgtgcggtat tcgtcatggg
    26341 cgtgtctgag ccgcagcaca ccgacctgcg cctggaggat tgccgccact gcaagcgcgc
    26401 ggctgcgctg gcggtcaagc tcgcccttgg cctcgatcaa gccggaccgc gccggatatt
    26461 gcagcagctt gaccaactgc caggaggcgc gtgccgccac cgaggtccag ccctgattga
    26521 gcaggaacgg gttctggtcg agcgcagcgc cagcgacgag gttgaggccc gggaacagtt
    26581 cgaggaaggc caggcgcgcc tcttcggcgg tgatccgctg ttcatagatg ctctgacgga
    26641 catcgggtct gttggtcagg gcaacgtggg ccagatcctc aaaactcagc cggcccggca
    26701 tcggaatcgg cgcctgcccc tcaatgctca gccgcagggg ttcggttgcc ggcaggttga
    26761 tgagcgcccg caactgatct tcggcgccaa ccagcgacct gcggtgcaga tcgatgtcgc
    26821 ggcggatcgc caacaggtcc cgctggacgc caagcgcctc gccaggctcg gtgaggcctg
    26881 cgaggctgag ctgggcggca ttggccagtg cgccggtcat ttcgtgatca agcgcccgca
    26941 gcccctgatc gagtttttgc agcgccgccg cccgccagaa gaccgcacgc gtttcctcga
    27001 tgatccgcgc ggcaaccttg cgatgctgct cggcggaaat cagactccgg tccgctgcct
    27061 gccgggcgcg gaaataggac acgccgaaat cgaggagatt ccaggaaaag cttaggtctc
    27121 cgctgcgcga cagccgctcc gacgattccg aatagaaacc cgaccgggtt ccgctggcca
    27181 ccgcgcggct gaccgaggcc gccgaattgg agcgctgata gacctgcgcc gaggcaacaa
    27241 gctgaggcag catttcggcc gagcgcaggg ccagatcggc ctcacccagc aacctggaca
    27301 tggcctcggc cttgagttcc tgattgaatt tcaacgcgcg ggccagcgct tcgtaaagat
    27361 ccagcggacg ggccagcggc tcgtgatcga aggcgatggc agtccggata tcggcgacat
    27421 aatgtgctgc atcgccggcg ccgagcggcg agggacgcag cgagcagccc gccaacccga
    27481 gggcggcggc cagagcagcc atcctgaccg aacgatagac ctgtccctgc attgcactcg
    27541 tcgcttctcg cccagcgatg attcatagtc tggtaacctt aatcgctggt aaaattggcc
    27601 cctgcccttc gtcccttgat gacaaaggcg tgcctttggc ggagacgctt ctacttgcag
    27661 ccaggctgtg ccagaaagcc ctgctcccgc cgtcactgac aggacattcg atgcgcgctc
    27721 ctctgctcac acttgccctt cttgccggtt tcagccttcc ccccgcccct gccttggcgg
    27781 gcagctgcga acggacaaac ctgcagcagc gcaaggtggt tctgtgcacc atggacctcg
    27841 atcggcagac ggttcgcacc tattggaagg cgccctcggg cgaggcctac ggatccttgt
    27901 cagacatcgg ccgcaaggca cagccacagc caggccgcat gatctttgcg atgaatgccg
    27961 gaatgtatca ctcggatctg tcgccggtcg gcctctacat cgagaatggc gcggtcctga
    28021 agggccccaa tctggcgcgt ggccctggca attttcacat gttgccgaat ggcgtgttct
    28081 atgtcgcccg ccgtcgtgcc ggagtcctgg aggcgcgcgc ctttgtcaga gccggtctgc
    28141 gacccgacta cgccagccag tcggggccga tgctggtgat cggcggccgc cttcatccca
    28201 ggatcaacgg cgggggaacc tcggcaaaga tccgcaatgg tgtcggcgtg cgcggctcga
    28261 tggcctattt catcgtcacc gacgagccgg tgaccttcac cgagtttgcg cgcatgttcc
    28321 gcgatgatct caaggcaccg aatgccttgt atctggatgg ctcgatttcc agcatctatg
    28381 ccccatcggt tggccgcgct gacagtttca tgccggtcgg accgatcatt gccggcttcg
    28441 atcgatgatg cgaatgggat gccaagtcgc gccggatacg cgagcagcgc actggaaagc
    28501 ttgtttctag agtttcggcg aattccaatt cagagaaagt gctctatctt cttgtttttt
    28561 cgtattttcc tcatgcgaac cggaatccac ctcgcttgaa aatgctccag tgtcgatggc
    28621 ggctcaaacc accttgagga aggttctaca cagggaaacg attcgccctg ctgccttgcc
    28681 atcgccataa ggcgaaatgc ctcgcgccat tgcggcatag gccgccggat cagtaagaag
    28741 ccggctacat tcctcgacga tcgcctgtgt ttctgtgccg gccagccttg ccacaccggc
    28801 attgactgcc tcggggcgct cggtctcggc ccgcagcacc agcaccggct tgcccagggc
    28861 cggagcctct tcctggatgc cgccgctgtc ggtaaggacg aaggtactgg ccttcatcag
    28921 ggctgtcaga tcgagatagt tgaccggttc ggtcaggtgg agccgttcga gaccgctgag
    28981 atgctcgaac accgggccgc ggatattggg attgggatgg acgggataga caaagtcgac
    29041 atcctcgaac cggtcatgca gactctggat ggcacggcag atattgacca gcggcgctcc
    29101 gaagttttcc cgccgatgcg cggtgatcag caccagacgc cgcccgggtc gcggaaaatc
    29161 gcagggcaga tcgcgcccgg cgacatcaag cagcgcatcg atcacggtat tgcctgtgac
    29221 atgcaccgtt gcagcatcgt ggcgttcgcc aatcaggttg ttataggagg cttcggtcgg
    29281 ggcgaagtga atcgccgagg catgtccggc gatcagccgg ttcatttcct cgggaaacgg
    29341 attggcaagg tcgtgggtgc gcagccctgc ctcgacatgg ccgaccggca cccggcggta
    29401 gaagcaattc agcgccgcaa ccatgacgga ggttgtatca ccctgcacca gcaccagatc
    29461 gggagcgaga tcgacaagca gcggatccag cctttggaag atccgcgccg atagcccggc
    29521 cagcgtctgg ttggcctcca tcacgtcaag gtcgatgtcg atgccgatgt cgaaaatgcc
    29581 cagcgtctga cggatcattt cgcgatgctg cccggtcgag acaaccaccg tggttgccca
    29641 gggttgccgc ttgagggcgc ggatcaccgg cgccatcttg ataccctcgg gccgcgtgcc
    29701 gaccaggcag acaattttct tcaatcccgc ttcggccata tccgttctcg tccagccgtt
    29761 gaatctctgc ggcagcgtgc tgccggggca tgactgggcc ggagcacggc gcctgccgcc
    29821 gatctgaccc gacagatcct tagcggttgc ggctcaacaa cccgttgcgg tctggcgagg
    29881 cagctcaaat atggcaaacg agggctgaag aaacgccgca acgtccggcc ggctcgaagg
    29941 atgccgatgg cctggccaaa ccagcgtcgg gttccccgca gccaccgcgc cgttcgcttg
    30001 acagtgggag gcaggaagtc ctacggctgc aaatctgcac cactttctgc cggaaaccat
    30061 caaaaatgcg ttttcttgtc accggcggtg ccggtttcat cggatctgct gtctgccgcc
    30121 gtctcgtgcg cggcggcgcc gaggttctca atctcgacaa actgacctat gccggcaatc
    30181 tgacgtcgct ggcctcgatt tccaatgccg gcaattaccg cttcggccgg atcgacatct
    30241 gcgacctcgg aacggtgaac gaggcgtttg agaccttccg cccggactac gtgctgcatc
    30301 tggctgccga aagccatgtg gatcgatcga ttgtcggcgc acgcgccttc atcgacacca
    30361 acatcgtcgg cacattttcg atgctggagg cggcgcggcg ttactggagc gcactgcctg
    30421 ctgcccagaa ggaccgtttt cggttcttac atgtatcgac cgatgaggtt tacggctcgc
    30481 ttggcgatac cggcctgttc acggaaacaa ccccctacga tccgtcctcg ccctactcgg
    30541 cctcgaaggc ggcggctgat catctggttt cggcctgggt gcgcacctac ggcttccctg
    30601 ccgtcatttc gaactgctcc aacaactacg ggccgtttca tttccccgaa aagctgatcc
    30661 cgctcatcat tctcaatgcg ctgcacggca agccattgcc ggtctatggc gccggcgaca
    30721 acatccggga ctggctctat gtcgaggatc acgcccgcgc gctggacatg atcgtgcgca
    30781 atggccgtgt gggtgagaaa tacaacgtcg gtggccgcaa cgagcggcgc aacatcgagg
    30841 tggtcagggc gatctgtaat atcctcgaca ggctcacacc cggcggagcc ccgcatgacc
    30901 ggctcattac cttcgtgacc gatcgcccgg gccatgacca ccgctatgcg attgacgcgg
    30961 aaaaacttga gaaggaactc ggctggcagg cgatggaaac gtttgacacg ggcctcgaaa
    31021 agaccgtgca ctggtatctc gagaatgaat ggtggtggcg ccccctgcgt gacaaggtct
    31081 atgccggcga acgcctcggg ctgacaagcg ggagcccggc ctgatgcggc tgatcgtgac
    31141 cgggcgggac ggacaggtgg cccgtgcgct gatggcggaa gccccggcgc tgggcgtaat
    31201 actcattccc cttggccggc cagagcttga tctgctgcat ccgaccggtc ttgacgctct
    31261 ttttgccgcg cataggccgg atggcatcat caatgcggca gcctataccg ccgtcgatca
    31321 ggccgagaac gactacgatg cggcacttgc cgtcaatggc gccggcgccg gcgcggttgc
    31381 cactgctgcg gccgccctga atattccgat catccagctt tcgaccgact atgttttcga
    31441 tggcacgaag ccaacaccct acatcgaaaa cgacccggta gcgccgatca atgcctatgg
    31501 ccggtcgaaa ctcgccggcg agcgatctgt catcgacgcc acgcccaacc atgcgatcct
    31561 gcgaacctcc tgggtctatg cggcaacggg taagaatttc gtcctgacca tgctgcggct
    31621 ggctgcggac cgctcttccg tgacggtcgt cagcgaccag agaggcgcgc caagctatgc
    31681 gcccgatatc gcccgggccg tgctcgacgt cgcccggaat ctggccacgc gaccggatga
    31741 tgccctcttg cgcggcattt tccatatgac cggcggcggc gagaccagct gggctgaatt
    31801 tgccacggag atattccgcc aatcggcagc gcgcggcggg ccgtcggcag cggtgacccc
    31861 ggttccgacc agcgcctttc cgacccctgc gcggcgcccg gccaattcca ggctcgattg
    31921 cggccggctg aaaactgtgc acggtgtggc cttgccggac tggcgtgacg ggctcgggcg
    31981 ctgcatggat acgatatggg cgcaccgatg acgattcggg ccgacgcgaa gagcaaatag
    32041 gggcaaggca atgaaaggga tcatcctggc cggcggcagc ggaacccggc ttcacccgat
    32101 caccctggcg gtctccaaac agttggcccc ggtctacgac aagccaatga tctactatcc
    32161 gctgtcggtt ctgatgctgg ccggcatccg cgacgtgctg atcatttcga caccgcatga
    32221 cctgccgatg ttccgcaagc ttctcggcac tggcaaggca tggggcatgg cgttttccta
    32281 tgccgagcag cccgttccca acggtctggc acaggccttc gtcatcggcg aagacttcgt
    32341 cggcgacgat cgcgccgccc tcgttctcgg cgacaacatc ttttacggtg ccggcctctc
    32401 caatctgctc gtcgaggcaa gcgcgcgcga aagcggcgcg acggttttcg cctatcatgt
    32461 gccggatccc gagcgctatg gcgtcattga tttcgatgcg acggggcggg cgctgtcgat
    32521 cgaggaaaag ccgaaactgc ctagatcgaa ctgggcggtg accggccttt atttctatga
    32581 tcgcgatgtg gtcgagatcg ccaagaagat caggccatcc gcgcgcggtg aatacgagat
    32641 caccgatgtc aatcgggtct atctggagcg gggggaactc aatgtccagc gcatggggcg
    32701 cggatttgcc tggctggata ccggaacgcc tgattcactg accgatgcat cgaatttcgt
    32761 cagggccatc gaaagccggc aggggcttcg gatctgctgc ccggaggaga ttgccttcga
    32821 tcaggggttc attgacgcca ggcagctgag aaggctggca cgggcgctcg gcaagagcag
    32881 ttacggcaaa tatctagagc aggtgctggc cgacgccgga tgatgcggca ggtgcggcag
    32941 caaagccgat ggggctgacc ggatgaaatt cgagcgcttt gatattgacg gaccggtgct
    33001 ggtaacgccg gtccggatcg gcgacagccg cggtttcttt tccgagagct ttcgcgctga
    33061 caaattcgag gccgagattg gcccgacgcg cttcgttcag gacaatcaat ccctttcggt
    33121 tgtccgcgga acaattcgtg gcctgcatta tcagaaggcg ccccatgctc agggcaagct
    33181 gatccgtgtg ctgcgcggcg cggttttcga cgtcgccgtc gacatccgca aacagtcgaa
    33241 aacctttggc cggcacgttg ccgtcgagct ttcaagcgac aatggccagc agctctgggt
    33301 gcctcccggc tttctccacg gcttctgcac gctctgcgac aatgttgagg tgatgtacaa
    33361 ggtgaccgat tattacagcc cggcttgcga tgctgcggtg cggtggaatg atcccgacat
    33421 cggcattcgc tggccgatcg cggaagctga ggcccaactg tccgcaaagg accagactgc
    33481 gccatggctc agggatattt caccgatgtg agaattgacg gcatccgcag cacggttttc
    33541 ggccgataga gcatgctgca attaagtgga agccggttaa tcgcagcatg ccttatacca
    33601 agtcgcaagg cgaggaactc gccgcgactt ggttcgtttc aaaatttctc actattatca
    33661 acgagatgag aaatttttga gttgcaccaa agagtcatta tcaatgactt gtggtattag
    33721 aggtgctttt gcggtgaatg accgacagat cagaaccgag gccactggca tgattgcagg
    33781 attgaggcgc tcatgaccat ctcctcgctc ttgaaggacc gcagattgtg gcttgccctt
    33841 gccgcaattg ccacgattgc gcttgtccgc tggtctggcg tcggagatct ccttgcgctt
    33901 gaaaccctca gggcgcatcg cgaaacgctg gcagcctggg tcagtgctca cgtcgtcatc
    33961 gccagcttag cctaccttgc aacctatgtt gtggtggtgg ccttctcggt gccaggcgcg
    34021 tttgtgctga ccctgtgggg cgggttcctg ttcggcagcc tgctaggaac cgcattgacc
    34081 gtgatcggtg ccacgatcgg cgcaacggcg atctttatct ttgccaggac gatttttgga
    34141 gaaaacgcgc tcgaccggtt cggcgtttcc gcagcacggt tggctgatgg cctcagacgg
    34201 gatgcagcgt cttatctgct ggtcttgcgc ctcgtcccgc tgtttccgtt ctttctggtc
    34261 aatctcgtcc cggccttcgt cggcgtgcca ctctccacct acgcaatcac caccgtcttt
    34321 ggcatcatcc ccggaactgc cgtgttctcg ctggctggcg ccggaatcgg ctctatcctc
    34381 gaccagggcg gcacattgac tccggggtcg atcctgacac cggaaatcgt cgccggtctc
    34441 gtcgggctgg caatcctgtc gcttatcgcc ataccgttgc gccggcactt tggctcaggc
    34501 gggggatgaa cagggccgtg ttgacgttgt cggcccatgc ttttctttcg cccctcgatg
    34561 gagccgccag cttgtgccga aagcctgaca cgcgatttcg gtattacagg acgcgtccgg
    34621 cgacagcatc gagcttggcc agcatatctg gatcccgtgc ttccggcggc gtgatgatgg
    34681 catgatcgag cgcgcgatgc gagccaacgg gacagtcttc ccgttcttct gggaaatccg
    34741 ccaccagttc gaccagcagc cgacgcgcgg cgagcatgtt ctgattcagc tgccggatca
    34801 ccatgtcgac gctgacatcg gcgtgatcgg aatgccagca atcaaaatcg gtcaccatcg
    34861 cgcacatgca ataggtgatc tccgcctcgc gggcgagctt ggcctcgggc atcgaggtca
    34921 tgccgatgat atcatagccc agacccttgt aataaaggct ctctgcccgc gttgagaact
    34981 gcggcccttc catgcagacc gccgtccctc cccggcgaat gtcgattcct gcccggcgtg
    35041 ccgcctcggc cacacgctgg atcagcctgg gaccgaacgg atcgccgaag gcgacatggg
    35101 cgacgcaacc attgccgaag aagctggttt gtcgagcaaa tgtgcgatcg agcaattgat
    35161 ccggaaggat aaaacgaccc ggtggcaagt cctccttgta ggagccgacg gctgaaaaac
    35221 tcacgatatc ggtgacgccg acacgtttca tcgcatcgat attggcgcgg tagttgatgc
    35281 ctccgggggc aaggcgatgg cgatcgccgt gacgcggtag gaaaacaata tccttgccgc
    35341 ccatccggcc aaagcggagt gctgccgaag gctgtcccca cggcgtctcg atcagctgct
    35401 ggtaaggatc attcaatccg ggcagatcat agagccctga tccgccaatt acaccgagct
    35461 ttgccttcat gagcaacatt cctgaggcca agacaatccg gccgtccgta tccagttacc
    35521 attgcagcag caggcgtcgg aagccagcgc cagtcagcct ggatcatact gtatttcttg
    35581 attgactcgt gacaagatgg aaaacgtgtt cgataataat aggttagaac gttttccgac
    35641 cggattgagc cacctgcgtt cccacaggtt cctgatgaca aaaggccgcc aatggatccg
    35701 ccgaagtaag ggtcggcgtc agggtcgcgc agtttctgaa aataattttg ttcaatttca
    35761 atggattaag ggtagaaatg gcggagaggg agtccgttag atttttgtct ttgtaggtct
    35821 ttttgaatct aattaccgta tctaatccaa tggcttataa aaattctctg tctccataca
    35881 gcttgcaatg cctgcataaa tccagtaatt tttgtgggac aagatgttgg acaaacgggg
    35941 ccggaccatg ccgaagaagg ctaaggaact cactgcgaaa gctgttgcga gtttgaaggc
    36001 agatggccgc tacgctgtcg gcggtgcgga cgggctgcat ctgcgcatcg cgggcgcttc
    36061 gcgcgcttgg gtgctgcgct tggttgtggg cacgcgcgtt gccgccaacg gacgccctgc
    36121 catccatcgc cgggacattg gtttgggtag ttgtgacgat gtgaccttgg cccaagcgcg
    36181 agaaaaagcc cgcgaactgc gcaggcaggt gcgggacggg accgatccgc ttgaagctaa
    36241 ggaagccgaa caacgcgcga agctggcagc gaaagccgaa gaggaacgcc gtaagacctt
    36301 cgaagagtgc gccgaagccg taattgagaa gaagggcgag gaattgcgta atgcaaaggc
    36361 gctggcgcaa tggcgtagca cgctcgaaaa atacgcatat cctgtactca atgacggtcg
    36421 gaaaattgac gaactgaaga aggaagatgt tctccgcgtg ctcaagccga tctggtcgca
    36481 gaaaaccgaa actgcatcgc ggcttcgcgg gcgaatagaa actgtctttg atcatgcgag
    36541 ggcaaaggac gcataccacg gcgacaatcc cgccgcttgg aaaggaatgc tcgaaccgct
    36601 gcttccgaag ccaaacaagg tcaagaaagt gaggcaccac gcggccttgc cctacgccga
    36661 aattggcgcg ttcatggtcg atcttcggaa gcgcgaaggt atgtcagcgc gggcgctgga
    36721 attcagcatc cttaccgccg cacgatcttc ggaagcgcgc ttcgcgactt ggggcgaaat
    36781 cgacatggat gcgaaggttt ggattttgcc gcgcgaacga atgaaggcag acaaggaaca
    36841 tcgggttgcg ctttgtgatg aagctatcgc cctgcttcgc gctctgccga ggatcgaagg
    36901 cgacgacatt gtatttccag ctccgcgcgg cggtgcgttg tcggaccaag cttttcaaaa
    36961 gctgtttgaa cgcatgggtt gggaaaacat cacgcaacac ggcttccgct caaccttccg
    37021 cgattgggcg ggcgaaacga cggcgcatcc gcgcgaagtg atcgaacaag cgcttgccca
    37081 cagcctcaag gacaaggccg aagccgctta tgcccgtggc gacctaatgg cgaagcggca
    37141 acgtcttatg gccgattggg gccgctattg cggtatcgtg cggggcggcg aaagcaacaa
    37201 cgtcgtatcg atgcgggaag cggtttgatg acaggtcgaa agccccgcgc gataatcccc
    37261 attgacgacg tttatatgtt cacggatgga cacgcgcgcc tgtcgaagtt tcgcgtcgaa
    37321 gctatcaaac tggccttgac cggaagcgac cttcgtaaag ttgccgaatt tatcgcccca
    37381 aaatgggcag ggcgatcatt tgaaacgaag acagaagatc aaaaggagga ttttatacgc
    37441 tacgtatata aagatattcg agactatatt aatgatttaa gtgatgaaga cagacaacgt
    37501 ataatgacgt cgtaaatttt tgccctgtga cggcgatttt tgctcgctgt aattcttcta
    37561 gcgccacata accgctaggc tcaggaccta ttaatttcca ttgagatgtg attcacggtc
    37621 tccataggt
//
LOCUS       DAOMXG010000003        37153 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_774_length_37153_cov_10.415440, whole genome shotgun sequence.
ACCESSION   DAOMXG010000003 DAOMXG010000000
VERSION     DAOMXG010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 37153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37153
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_774_length_37153_cov_10.415440"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1126)
                     /locus_tag="PLQ11_00450"
     CDS             complement(<1..1126)
                     /locus_tag="PLQ11_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRK23400.1"
                     /translation="MKAAHLNPAEYFHKVVDCQWACPAHTPVPEYIRLIAAGRYSDAY
                     MINWESNVFPGILGRTCDRPCEPACRRGRVEKDPVAICRLKRVAADNKDDIRARLPKK
                     AEQKNGKRIACVGAGPASLTVARDLAPLGYEVVVFDAEPKAGGMVRTQIPKFRLPERV
                     IDEETGYVLDLGVEFRHGHRIDSLKALLAEGWDAVFVGSGAPRGRDLDVPGRKEAARH
                     IHLGIDWLANVAFGHITSISKRVIVLGGGNTAMDCCRSARRMGGEDVKVIVRSGFEEM
                     KASPWEKEDAMHEGIPILNFMVPKEFRHDNGRLTGMVFEKVKAEYDARGRRNLIPTGE
                     PDTFVPCDEVLVAVGQENAFPWIERRQLLLVDRVGRQLEVH"
     gene            complement(1317..2249)
                     /locus_tag="PLQ11_00455"
     CDS             complement(1317..2249)
                     /locus_tag="PLQ11_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRK23401.1"
                     /translation="MHFDLSDLRLFVGVAEAGNLTRGARLSFLSAPAASARIKGLERQ
                     LATRLLWRDARGVRLTDAGETLLRHARVILRQVEHLKHEFGAAAGATGHIRIAANTTA
                     VTEFMPELLARFLAERPGVTVDLQERLTQDIVASVLDGSADLGIVSGRVPTEGLQALH
                     FSTDRLVLATPLLHPLAGRGGVAFAETLAYEHIGLHEGSTLLAFIRALMDQHGHDRPL
                     RIQVRSFEAMCRMVEAGVGVGIMPESAAIRHSKTMRIATAALVDSWALRERCVLVREI
                     EALPASARDLVDRMLRSGSGGSSVTSVASGHDKD"
     gene            2367..3212
                     /locus_tag="PLQ11_00460"
     CDS             2367..3212
                     /locus_tag="PLQ11_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HRK23402.1"
                     /translation="MAVDMALLNQWLGKTERFTERLSPFPSNALAATLDRCDPPYDVG
                     SDLPPLWHWLHFLPISPLAQAGPDGHPARGGFLPPVPLPRRMWAGSRLTFLRPLRIGE
                     SVDKLSRVTKIEHKSGRSGELVFVTVRHEFGGAAGPAIEEEHDIVYRGAAVPGAPAPA
                     PVPAPAHSEWRRPIHPDAVLLFRYSALTFNSHRIHYDLDYVTKVEGYPGLIVHGPLIA
                     TLLLDGLRRAHPGKRVTAFSFRAIAPTFHIDEFAVCGQLTGGRAQLWADKAGALTMDA
                     TADIA"
     gene            3268..4428
                     /locus_tag="PLQ11_00465"
     CDS             3268..4428
                     /locus_tag="PLQ11_00465"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006932631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRK23403.1"
                     /translation="MTRPDDAHQDIRDGVRAICAEFPAEYHRKIDEARGYPEAFVDAL
                     TKAGWMAALIPEEYGGSGLGLTEASVIMEEINRSGGNSGACHGQMYNMGTLIRHGSEE
                     QRRRYLPKIASGELRLQSMGVTEPTTGTDTTRIKTTAVRKGDRYVINGQKVWISRIQH
                     SDLMILLARTTPLDQVKKKSEGMSIFLVDLAEAQKKGLTVRPILNMVNHETNELFFDN
                     LEIPAENLIGEEGQGFKYILTGLNAERTLIAAECIGDGYWFIDKVSAYAKERIVFGRP
                     IGQNQGVQFPIAESYIEVEAANLMRFEACRLYDAHQPCGAQANMAKYLAAKASWEAAN
                     ACIQFHGGFGFACEYDVERKFRETRLYQVAPISTNLILSFVAEHILGLPRSF"
     gene            4470..5933
                     /locus_tag="PLQ11_00470"
     CDS             4470..5933
                     /locus_tag="PLQ11_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRK23404.1"
                     /translation="MNKPVTLTPAQGVFINNRWQPSRSAKTIAMIAPAEGRAFTEIAA
                     GNGEDIDLAVAAARKAIELGTWGRLTATERGRLLSRLSRLIDDHAEELARLESRDTGK
                     PLKQGRADIVALSRYFEFYGGAADKVHGETIPFLDGYLVNTLYEPLGVTGHIIPWNYP
                     AQMFGRTVAAALAMGNATVIKPAEEACLVPLRLAELAAAAGFPDGAVNVVPGLGEEAG
                     AALAAHPGIDFISFTGSPEVGTLVQTAAARNHIGCTLELGGKSPQIVFADADLDAAVA
                     SVAAAIVQNAGQTCSAGSRVLIERSLWDRFLADLALRFGKIRAGTPEMDLDLGPVISA
                     GQKARIEAMLARAEAQGAKILASGQIAENVPKDGFFVTPLVYHHVDPASELVQQEVFG
                     PVLAAMPFDSEDEAIRLANGTAYGLVAGVWSKDGARALRVARKVRVGQVFVNGYGAGG
                     GIELPFGGMKKSGHGREKGFEALYEFGALKTMIVKHG"
     gene            5948..7351
                     /locus_tag="PLQ11_00475"
     CDS             5948..7351
                     /locus_tag="PLQ11_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009209360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HRK23405.1"
                     /translation="METTLSITGSIARFGSDPSLVPTESARAVARLSLMDWAAAALAG
                     LDEPVARITRELADEEAGAAQATLFGLARKVPARMAALANGALSHALDYDDTHFAYVG
                     HPSVAILPAALAVAERQGASAAAFLDAALIGMEAACRIGHWLGTQHYNHGFHQTATSG
                     CFGATVAAGRLMGLDTAAMRQALGIATTRASGLKSQFGTMGKPFNAGIAAANGVEAAL
                     LAARGFVSRPDGLECLQGFGETHAGERTDLAGALAGLGNDFWFEQVQHKFHACCHGLH
                     AGLEALAALKAAHHLQPGSIAGVTITINPRWLRVCNIASPTTGLEAKFSYRLTTAMAL
                     AGVETAALQTYSDAICSRPDLMALRDRVVVRGDASLPDTAARVALQHTDGRALDGAFD
                     LDQPMPLSQRRERIAAKVRSLLAPQAAEAILALDSGLGRLGAIQVAETLGRLALSHPC
                     AAVMSPASSDRSSHASA"
     gene            7338..8516
                     /locus_tag="PLQ11_00480"
     CDS             7338..8516
                     /locus_tag="PLQ11_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002966418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HRK23406.1"
                     /translation="MRPLDGITVVTLEHAIAAPFCTRQLADLGARVIKIERPGVGDFA
                     RAYDARVKGLASHFVWTNRSKESLTLDVKHPAAAGILATLVEKADVLVQNLAPGAAAR
                     LGLSYDAVRKHNPRIIVCNISGYGEDGPYRDRKAYDLLIQSEAGFLSVTGTPDEPAKA
                     GCSIADIAAGMYAYTNILAALIQRGRTGKGCSIDVSMLESMVEWMNYPMYYAFDGASP
                     PARAGAAHATIYPYGPFPAGDGKVVMLGLQNEREWAIFCDKVLLQPEIAKDPRFTSNA
                     KRAANRTALRSLIVKAFAGLTAEALIARLDAAGIANSRMNDMHEVWQHAQLKARNRWR
                     EVMSPSGPIPALLPPGRTSSFEARMDGVPALGEHTSAILAELGLDMTRIKALQAEKAI
                     "
     gene            8616..9605
                     /locus_tag="PLQ11_00485"
     CDS             8616..9605
                     /locus_tag="PLQ11_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRK23407.1"
                     /translation="MGAAAAAMLAGSSLAALAQSPIVIKFSHVVANDTPKGKGAIKFQ
                     ELAEKYTAGKVKVEVYPNSQLYKDKEEMEALQLGAVQMLAPSLAKFAPLGVKEFEIFD
                     FPLIFKDKEALGKVTKGAVGKGLLDKLQDKGITGVAYWDNGFKIFSANKPIKSPTDML
                     GQKIRIQSSKVLDAQIRAVGAIPQVLAFSEVYQALQTGVVDGQENTPSNMFTQKMHEV
                     QKHAVMTNHGYIGYAVIVNKKFWDGIPADIRAQLDKAMAEATEYANTISDSENADALA
                     EMKKTGKINFYEPTKAEMEAWQKAMLTVHKDMTSRVGAEIVKQVYTAVGLPTN"
     gene            complement(9662..11158)
                     /locus_tag="PLQ11_00490"
     CDS             complement(9662..11158)
                     /locus_tag="PLQ11_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020810026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HRK23408.1"
                     /translation="MTTITEADLIQSLADALQYISYYHPEDYITNLVTAYEREESPAA
                     KQAMAQILINSRMAALGKRPMCQDTGSAQIFIRLGVGVRLATQRSLQEIADEATRIAW
                     QVSGNPLRASMVADPIFERKNTRDNTPAMLHVEVVAGDRLEVHVAAKGGGSENKAKFA
                     ALEPSDDIVAWVLKSVESMGAGWCPPGIMGLGVGGSPDKTLILAKSSLLDPIDMPDLL
                     RRGPSSKTEELRIELFHKINALGIGAQGLGGLSTVLDVKIRTLPTHAASKPVGLVPQC
                     AADRHAHFVLDGSGPAALKPPNLALWPDLKLDGPAGNVRRVDLDRLDSAQVQSWKAGD
                     TLLISGRLLTGRDAAHARMARMLAAGEKLPVDFAGRAIYYVGPVDAVAGEAVGPAGPT
                     TSTRMDRFTDMMLGQAGLAVMIGKAERGPEAIESIRRHKAAYLIAVGGAAYLVSKAIR
                     SARVLAFADLGMEAIHEFVVEDMPVTVAVDSTGASVHTLGPARWRRSL"
     gene            complement(11262..12335)
                     /locus_tag="PLQ11_00495"
     CDS             complement(11262..12335)
                     /locus_tag="PLQ11_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRK23409.1"
                     /translation="MRAAFLTGHGGNEVVTAGDRPDPVPGPGEVLVRVEAATVNRVDL
                     YMRNSGAGITHSLPQIMGVDGAGTIISVPAGESGLQPGQRVTLYAGVTCGRCEFCRRG
                     DRPLCLGARYLGEHRDGTFAGFVCLPAENVFPLPDMFSFAEGAALGITHLTAWRMITG
                     KARMQPHETALIFGIGGGVALAALQLIQAMGGRAIVTSRDPTKLDLAARFGALAGIDG
                     GREDVVRRVMDLTGGRGVDVVIETIGEAVWPQALRALVRGGRLVTCGATSGSHPSADI
                     QRLFIRQLQVFGSTLGSLAEFADLITFCERHDIRPVIDRVYALDDIHAALDRHDSGAN
                     TGKVVIAPHNQATAHSRACCEKV"
     gene            complement(12427..13614)
                     /gene="uxuA"
                     /locus_tag="PLQ11_00500"
     CDS             complement(12427..13614)
                     /gene="uxuA"
                     /locus_tag="PLQ11_00500"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011649592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HRK23410.1"
                     /translation="MRQGWRWFGPNDPVSFDDIRQAGASDVVSSLHHLPIGQGWTRSE
                     VSRHKALIETTPAGRTPLAWSVIESIPVPDDVKRLGAAATRSIAAWIASMEAAAAEGL
                     RIICYNFMPVVDWTRTELDYVMPTGATALRFDHVKFAAFELFILQRPGAERDYSEDVR
                     VKARKVFSAMSQADVDYIVMVIASALPGSTTEPMTIPMFRDKLKQYERIDAAVLRRHL
                     IEFLGAVTPRAEELGVRLTIHPDDPPRPLFGLPRVASQQSDFAALFDAVPSPANGLCF
                     CTGSLGVRADNDLPAMARRFASRIHFAHLRATRREADGLSFHESDHLDGDVDMVSVLR
                     ELLAEDRRRASSESIVFRPDHGHRMLNDIKAERVTPGYTAIGRLKGLAELRGIIRALD
                     AGA"
     gene            complement(13757..14719)
                     /locus_tag="PLQ11_00505"
     CDS             complement(13757..14719)
                     /locus_tag="PLQ11_00505"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRK23411.1"
                     /translation="MPQHRAARILVTGAGGFIGKVIVDRLRHAGHETRAGVRRRPPAQ
                     AGCLIADLDRPETLAAALRDVDLVVHAAYGDVVRMADQCGALLAAMTHAAACRLVLLS
                     SVAVYGNLEGPIDEHATLPPPIDGYAAGKLACETMVGHWTEQAGNRAIILRPGIVYGK
                     NSPFWIDRMVERIRLGGWGTFGAAGEGIAALIHVDDLAAMCLSAVQELEGESGQSISM
                     LNAIGPETPSWNDYFSSLAAAIGAPPLQPIAAPALRWRQALAVPAKVWRKLGMPGMAA
                     AASAPMPGEVALFMRKARYDMAAARQVLGFEPQVGLAEGLGRSL"
     gene            complement(14722..16200)
                     /locus_tag="PLQ11_00510"
     CDS             complement(14722..16200)
                     /locus_tag="PLQ11_00510"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRK23412.1"
                     /translation="MAESLRRQGATGSRLIGWLGWAGLDALGRLALLTGSTVVFSRLL
                     GAHEFGVSALVLAIVAAASLFVGAPFEEALTRQRGLRRRHLRAALGVSWVAAIVLIAA
                     SGLAGRWLATVFGEPEIALLLPVTMVSIVFSAHSDIMTALARRLKRFNDVAYATLMGH
                     VIGICLALGLAFAGFGIWALIGQRVLVVLARAILLQRALGYLILPLWSPADLRGLGRF
                     AGLSFLSRLTENLGYLAFNAIVQVIYGTQVLGHVNMAMRLIEPIRGAILATGHNLAFS
                     YFARSPDRHRLRERARDVVAKAALVTVPAFVGLAVVAPDLIPMMAGPGWEPAIALAIC
                     LSLASALAVPSALVYTAFSAAGRPEHSLASLVLGLISSVIILVVCAPLGPISVGLSRL
                     AGDGLRALCAVVLPAAHLGWTRLARLDALAHAWLLSALMGLVTILLAAPAAAASHVLS
                     LAIAVLLGVAVYALLLVAFARSRLAEMARLAGIPVPGFLERT"
     gene            complement(16193..17554)
                     /locus_tag="PLQ11_00515"
     CDS             complement(16193..17554)
                     /locus_tag="PLQ11_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GumC family protein"
                     /protein_id="HRK23413.1"
                     /translation="MAAAYRHYSPVRYPATAQLLFDPRGLKVFNNDLTSGYHDANAAI
                     SYVESQMGVIQSERVLSRVMGAECDELRELAKTATHSVYKASGHGFTRFCPREGWDGD
                     WQRGLQEMRRVLTVKRVERSYVVDVTATGATPQLATSLASAVVNAYIQEEAATRAETI
                     NRLTGDLSGRLSDLRKAVKDSEDRLDHYRRDKNLLRVGDRLIVETSLATATAALNESQ
                     TRIDRARARMRQIETAARNPSALGALGAEADTRQLLSLYERRSAVMAELAPLFARAGP
                     RHPALIDARSRVAEIDRAISHEMSAIRVAARSDLSRSEAEQGNLSKTVSELTVAVATA
                     KQSEIALRELEQSLEANRKVLESFETRMREAREYGKLDSANLRIVSVARSPLPQNPLP
                     KLLSFALLGWVLGIVLAVVGVAIWALVQSMRHPPHQLGGNSANPAYALQMRAEAFARY
                     RYG"
     gene            complement(17870..19261)
                     /locus_tag="PLQ11_00520"
     CDS             complement(17870..19261)
                     /locus_tag="PLQ11_00520"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HRK23414.1"
                     /translation="MAVLFTRLCFSLVAMLVDAATILAAMLASDFAYNEIVHDAGSVC
                     VANIQIGLFAAILFVGSNLLRNSYSLADYLDLSGHARQVFSQWNLAFLAAATFGFLAR
                     VIEDTSRGTFLVFYVAGLCALFLGRAGLVHLMKRNAVAGSALAARVVIVGFAKDIDDF
                     ISMQQPEQQGMLVVARHTIAEDRAIRDTDLAEATTMIRRLMPDDIVIAVPWNRTDTIE
                     ACVTAFLRVPASIHLHLDPDSPLNRFGGANMGANGAISGFRLRGYAIGTLGSVLKRGS
                     DIILSALALIMLAPVFLAIAVAIRLDSPGPVLFRQMRHGFNKEPFRILKFRSMKVAED
                     GPVVTQATADDPRITRVGRWLRRYNLDELPQLLNVLMGQMSLVGPRPHALVHDDAFEQ
                     NIVLYARRHNVKPGITGWAQVNGLRGETDTPEKISQRVHHDLYYIDHWSFVLDVWILF
                     LTLFSRKAYRNAG"
     gene            complement(19693..20619)
                     /locus_tag="PLQ11_00525"
     CDS             complement(19693..20619)
                     /locus_tag="PLQ11_00525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRK23415.1"
                     /translation="MSVSIVVPAYRATGTLARTLLSCREAVRPPDLIVVLDGEDAPLE
                     DVARTTIPGAKVVVLPAGSGAPMCRNTGLRLVSSPYVMFLDADDYIEGQLLASAAEAA
                     GKDAADVVLGSFSFEYPDGRRDAHNPATRYEDLSCGTVLKRWLSGGYTPPCSVIWRTD
                     YVRTLGGWDETLAKNQDGDLIHRALMQGVRITTAPKGQGIYVQDDNPGRITRKQTPRT
                     LQSQIRALDKIRIRLEELPFDPQRELALAYYALARLGYNNGMDDLGAIAEAAARDLGL
                     SGQHGSPGHVALASLLGLRNKQRLARFVRSLS"
     gene            20856..22019
                     /locus_tag="PLQ11_00530"
     CDS             20856..22019
                     /locus_tag="PLQ11_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020179866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRK23416.1"
                     /translation="MAASDITSIAFVGCGYVADSYRHCLPLHRQSLQLAGVYDRDPER
                     LAAFRATWNDHAYDSLEALLADPAVSLIVNLTPPEAHAEVTLAALAAGKHVYTEKPLA
                     MTTAEAIRLRDAAIKAGLRIGAAPCNLLGEASQTLWKAVREGRIGRPVLAYAELDDGM
                     VHRANYRQWISRSGRPWPAHGEFETGCTFEHAGYAITVLAGLFGPVRRVTAFSALLIA
                     NKDTDQPLDHPAPDFSVGMLEFDGGVVARITNSVVAPYDHRLRIIGESGTLEIAEPWD
                     YASPVRLKKPAETRLARLLERRFGRALGGETIAPARPVPFSGGRGRPTMDFLRGVAEL
                     AQSIREGRRSRLDEDFAVHVTEVTEILQHPDRFSRPQVVQSSFKPIMPMDWAD"
     gene            22022..22954
                     /locus_tag="PLQ11_00535"
     CDS             22022..22954
                     /locus_tag="PLQ11_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020179867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6492 family protein"
                     /protein_id="HRK23417.1"
                     /translation="MSDKPYSAGIVTCSYSGDYDACRILCESIDRFVPAAMAHTLYVP
                     RADVPMFAHLGNARRRIATQEELLPGWFWKLPLPSPEWRARLLLPRRNVYLTPFSLPV
                     RGWIAQQVMKLAATQRAETDIVVNIDSDNAFIRPLTMDHLVRDGRVRFYRNPDKVPLE
                     SHRRWHRAAGKLLGLPDSDFYGAEYIHPLVVWRRDVLRAMLDRIVEVTGMDWQKALAR
                     TTHFAEFVLYGVYADKVAGLDQAGLWASDQPFCLSRWAEDFRSAADEVAFVNELQPRH
                     VTCLIQSTIPMPPNERQRIFNLATDAAAAMDRAG"
     gene            23033..23674
                     /gene="maiA"
                     /locus_tag="PLQ11_00540"
     CDS             23033..23674
                     /gene="maiA"
                     /locus_tag="PLQ11_00540"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HRK23418.1"
                     /translation="MTLYGYFRSSAAYRCRIAFNLKGLKPNLVSVHLRKGEQRADDFL
                     KRNPQGFVPALETAGQVLTQSLAIIEWLEETHPEPALLPRDPFDRALVRALALTIACD
                     IHPLQNLRVLTHLRNHLGQDQAGIDAWCRTWIESGLGAYERMLVDKGCSGGFSFGATP
                     TLADACLIPQVFSAERFGADLTAMPTIRAIADHAGQHPAFAAAHPARQPDYEA"
     gene            23837..24883
                     /locus_tag="PLQ11_00545"
     CDS             23837..24883
                     /locus_tag="PLQ11_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009451631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRK23419.1"
                     /translation="MTFNRRHFSAALAAAVAAPAVFSGARAQAQVTLRLHHFLPPVSN
                     GQAKLLGPWSKKIEAESQGRIKIDIYPSMQLGGTPPQLFDQARDGVVDIVWTLPGNTP
                     GRFPTTEVFELPFICAEKGIVNAKASQEFANSFLKDEVKDVKLLAYWLHDAGHIHANK
                     QVRTMDDLKGLKLRNPTRLAGEALKALGATSVGMPVPQVPESLAQRVIDGAVIPWEVV
                     PAVKVHELVKFHTEITGSPTLYSASFFLAMNKARFDNLPDDLKAVIDANSGMAFADMA
                     GKMWDEEAVRVRDMIAKRGNTITQLAEDEKQRWIKATEPVYGAWYEQMKARSLDGPKM
                     VEAARALVAKHAKA"
     gene            24936..25448
                     /locus_tag="PLQ11_00550"
     CDS             24936..25448
                     /locus_tag="PLQ11_00550"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRK23420.1"
                     /translation="MADQEPPASGRDKRLERITGWLATFGGLLALAVAMLVAVSVLGR
                     WLFSAPIDGDFEFVKMATAVAVFSYLPYTQARCGNIMVDTFTTSLSPRINRRLDAFWD
                     FVYALFVGGMAVALAMGTLDALRSGETTMQRQILLWPSIGATAVLCLVLAMTALTTGM
                     ALLKDRQDRG"
     gene            25448..26890
                     /locus_tag="PLQ11_00555"
     CDS             25448..26890
                     /locus_tag="PLQ11_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007199167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRK23421.1"
                     /translation="MSGTGVAFIGFGVMLVMLALRAPVGLAMLVVGAAGYIHLSSAQA
                     FFAYMKTNAYHQFANYTLSVIPLFILMGALAERAGIAASLFKAAERGFGRMRGGLAMA
                     VIGACTAFGAICGSSVATTATFGRAALPELLKARYSGGLATATIAMGGTLGILIPPSV
                     ILVVYAITTEQNIAKLFQAALIPGLLAALFYCMTIAIVARLRPEQAPEGIPSELEPAS
                     HPAWIRLLAAALLLAAGAAYLSGHLGLWSAAGLAGLGLAIAIVDAAIMPAMTIAIIVV
                     GGIYGGVFTPTEGAAVGAIAMLGVGLANRSLSAGDILLAVRQTAETSGMIFIILLGAE
                     IFGAFLALSRLPTQAAEMIGSSGLSPTLVLAGMMVFYILLGAVMDELAMILLTLPVFF
                     PIVQALDFGMLPDEVAIWFGILVLIVVGIGLTMPPIGLNVFVISSLARTVPIKAIYIG
                     VMPFLVADFIRLGLVLAFPSLTLWLVRLLQ"
     gene            complement(26915..27496)
                     /locus_tag="PLQ11_00560"
     CDS             complement(26915..27496)
                     /locus_tag="PLQ11_00560"
                     /inference="COORDINATES: protein motif:HMM:NF016180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit gamma family protein"
                     /protein_id="HRK23422.1"
                     /translation="MVEGAPGKSPTQSGAAAGLILPVSPACTVRMAEERSRFVLQGPD
                     AARTMAGRAFGVALPVNPLEVMTGADGSRAALWLGPDEWLLLAPATEAAMLGEELGKA
                     LAGSPHALVDVTHRQMGLCLEGPLAARLIAAGCPLDLRLKSFPAGRATRSVFHKTEIL
                     LWRQTEHRFHIEIWRSFLPYLVGHLHHAMTGLD"
     gene            complement(27489..30479)
                     /locus_tag="PLQ11_00565"
     CDS             complement(27489..30479)
                     /locus_tag="PLQ11_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit alpha family protein"
                     /protein_id="HRK23423.1"
                     /translation="MKQSFRTAAGGRIDRTSPLRFSFDGLTYRGFKGDTLASALLANG
                     VHFVGRSYKYHRPRGILSAGIEEPNALVSVHRGKGRMTPNLRATEVPLHDGLEARSQN
                     NWPSLGLDIGAINDRFSRFLSAGFYYKTFMGPKMAGTTLWTRLFEPIVRHAAGLGARP
                     VDPDPDRYVQYYDHCDVLVVGGGPSGLAAALAAGEAGADVILCDEQSELGGSLLAEAR
                     CGIDGQDAAIWRDATLSRLMQLPNVRLLPRTQGFGLYGHNLVGLVERMDDSGPAPGER
                     PSQRLWQVRAGRVVLATGAIERPLVFSGNDRPGIILADAARIYLNRYGVRVGSRIVVV
                     TAHDSAYRAALDLHQAGITVAAICDLRPAARGALPAEARAAGLKVIEEAIVDGTRGRR
                     RVSGVLIRAGADERVGQAMELDCDAVLMSAGWTPSLHLSSQARQTPRWDAERQLFLPG
                     GGNSGIVSVGACNGVFGLAEALAEGHAAGCASTGQAPGRHFAVTGDPPQTGGWLGLQP
                     GDTDSGEAFVDFQNDVKAADIELAVREGYRSIEHVKRYTTTGMATDQGKTSNLNALAI
                     AARALGRSLPDVGLTTFRQPYTPVAFGALAGAHRDALFEPVRHTPMHDLAAAAGAVFE
                     DVGQWKRARYFPQHNEDLHAAVARECRAVRQAVGLFDASTLGKIEVCGPDAATFLERI
                     YCTPVASLRPGRCRYGLMLGETGFLMDDGVFARLDVNRFYLTTTTGGAARVLHHMEDY
                     RQTEWPDLDVWLTSVTEQWAVVAVQGPRARDLLAPLIQGFDIAAAAMPHMAIQEGQVA
                     GIPARVLRVSFTGELGFEVHVPAGYGRALWQVLRQAGEGHRAVTYGTEAMHVLRAEKG
                     YIIVGQETDGTVTPQDLGLAWAVGKSKGEFIGRRSLKRPDLVACGRKQLVGLLTDNPA
                     VVLDEGAQLTASAHPAPGSAALGHVTSSYWSAALGRSIALALLAEGRARQGEGLFAST
                     LSGSVPVRVTAPVFVDPEGSRLHG"
     gene            complement(30476..30769)
                     /locus_tag="PLQ11_00570"
     CDS             complement(30476..30769)
                     /locus_tag="PLQ11_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008507742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HRK23424.1"
                     /translation="MLLIRCPYCDMDRPEIEFRYGGEAHLVRPENPDSVSDDQWAEFL
                     YVRTNPKGLHAERWRHIHGCGRFFNALRDTVSDRFITTYRASEPRPDLPETMA"
     gene            complement(30777..32027)
                     /locus_tag="PLQ11_00575"
     CDS             complement(30777..32027)
                     /locus_tag="PLQ11_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007008824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit beta family protein"
                     /protein_id="HRK23425.1"
                     /translation="MRFSALSVLRQGLKKNAGWPAQWRSPEPKSAYDAVIVGAGGHGL
                     ATAYYLAREHGLANIAVVEKGWLGGGNTGRNTTVVRSNYLWEESEALYEHAMKLWQGL
                     SHELNYNVMYSPRGVMMLAQTHHDVQVLKRHVHANRLAGIDNEWLTPEQAKDFCPLLN
                     ISGDLRYPVLGATLQRRGGTARHDAVAWGYARAADRLGVDILERCQVLGIRRDAAGAA
                     TGVETSRGFIATRRIGVVAAGHTSVIMAMAGVRLPLESYPLQALVSEPVKPMMPCVVM
                     SNAMHAYMSQSDKGELVIGAGTDAYTSYSQTGGLHIATHTLDAVCELFPAVRRLRMLR
                     SWGGIVDVTPDRSPIIGLTPVPGLFVNCGWGTGGFKATPGSGHVFAHTLARGEPHPIA
                     APFSLDRFRTGRLIDEAAAAAVAH"
     gene            complement(32091..33722)
                     /locus_tag="PLQ11_00580"
     CDS             complement(32091..33722)
                     /locus_tag="PLQ11_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 44 protein"
                     /protein_id="HRK23426.1"
                     /translation="MSRAQPDRRNVLFGSLAGVVATGLPGRIEAGLIPSLTIDDTGPG
                     RPISPLIYGSNEIGVMDGGLASAVLDAAAGVTARRLGGDLMTGYNWLNNACHAGKNHH
                     HSNGPFLLEALQVPASDWDRPAAVIETMHAASLAIGAKSLVTLPLSPYLAADRLGRVG
                     EAEATPSPRFVPANWSLTDVPGTVSLPRLVAHLAERYGSSASPGGIHGYALDNEPGIW
                     HQTHPRLFPGRLQILAFIDRSIAAARAIKTIDPSARVFGPSSWGATEFVTFQNAPDWP
                     RHRAYGSFLGLYLDSFRRASEQDGRRLLDALDIHWYAFLEKGGLFRSEDPAHDQARLD
                     APRSLSEPGFVEQSWVPRALAQSGGALSLPLLPSLHRLIDRWFPGTALAITEFNYGGA
                     GQLTSGLALADALGRFATGGVAMATHWGSLPGWLGEAYRLYRQADPAGRRFGDRALEL
                     STLGAAEVSAYAASDAAGLALILINRSQRPVVLDIGLASGRAFAPGTMLGFDAGTGPT
                     RPLMPETTPQQGGWRLLLPPLAARRLRLNLALARF"
     gene            complement(33719..34648)
                     /locus_tag="PLQ11_00585"
     CDS             complement(33719..34648)
                     /locus_tag="PLQ11_00585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020179871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRK23427.1"
                     /translation="MDIVSVVIPTLNRQPSLKRALASLMQQRLDEQTAMDIIVVDNSV
                     DANARFLVEAMAEDGAPLRYFSEPVPGVATARNAGVAAATGRWVAFLDDDEQARPDWL
                     ARLVEVARSTGADAVFGPVLARSDGVHEIGGFAPYFCRRIDRADRADITDLAAYLGTN
                     NSMFDRARCLAGAGTFETSLNETGGEDSLLLKRLVLTGRRFAWAAEAEVLEWAAEKRL
                     NWAYVRKRKFLSGQIRVFVHHMVQPVEWGGIAKWMAVGGIQAVGAGLLAVALWPFARA
                     RAERAAATAWGGLGKVFWASRFRPSMYGSGLVS"
     gene            34770..36122
                     /locus_tag="PLQ11_00590"
     CDS             34770..36122
                     /locus_tag="PLQ11_00590"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRK23428.1"
                     /translation="MTDISSLKASFIIPGQAPSAAGTGRWRARLHGLLLFGFLLFTFV
                     GTRPFAEADAATRTDGNVLDRIAVLGLFGLSLLVLWLHRWRVARFALPALPVFVLVGF
                     AMASIVWSDFPDLTLRRSLLLFFLAVIAFAVAIGTENLRQLHTWMFATMTAIILINLA
                     GCVIVPSIAISEIGVRGMYSQKNVAGIVALITMILGTTWVLGSATRRETVIGMTALVP
                     TAFFLVITISKTSINLAALGIVVILFFGMIERHGRSFILFAIAVGLLLAVGITLWLAT
                     LEFDINAAMTTLIGDTSFTGRDELWAFARNAAEKRYWLGHGYGAFWDVGTANDPLIRA
                     EVGSWLAAVQIGVINQAHHGYLELWLHLGLPATVLATVITIWAALYGGLHACFGLAKP
                     ATRAALGAFAVLLLLYLAHNLTEATLFMRGASFNSTALLATFLIARARDFAFQDLAQP
                     "
     gene            36119..37093
                     /locus_tag="PLQ11_00595"
     CDS             36119..37093
                     /locus_tag="PLQ11_00595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRK23429.1"
                     /translation="MTTRTRLAIVLCTFHREALLAKALDSLSQQPIPQGLEVEILVVD
                     NSDAGTAREIVNQKAAASPVPMRWLAAHPANIAVARNAGVAATSADFIAFLDDDQQAA
                     SDWLRAVAENVRNGAIDAWFGAITPQFEAGSALTPMTRTLFSRWTDRPAGDRLVALGP
                     GKTAEVSLSTANSLFRRAAMLIGPGPFDPAFGNGGGEDYDLICRMQQRGSKMTWQPEM
                     QASEFVPASRCDPSYLRGRFYAGGQAFAEAAAKASAAPGLMRWIVRAKALVQAGLLSL
                     KLPAAAWRGGASWLDHSYVWAGVLGKLSFGQIKPIYRASDPRPAEANR"
BASE COUNT         7075 a        12087 c        11573 g         6418 t
ORIGIN      
        1 cgtgtacctc gagctgccgg ccgacgcggt cgacgagaag aagctggcgg cgctcgatcc
       61 acgggaaggc attctcctgt cccaccgcga ccagcacctc gtcgcagggc acgaacgtgt
      121 cgggctcgcc ggtcgggatc aggttgcggc ggccgcgggc gtcatattcg gccttgacct
      181 tttcgaacac catgccggtc agccggccgt tgtcatgccg gaactccttg ggaaccatga
      241 agttgaggat cgggatgccc tcgtgcatcg cgtcctcctt ctcccaggga gaggctttca
      301 tttcctcgaa gccggaacgg acgatcacct tcacatcctc gccgcccatg cggcgggcgg
      361 agcggcaaca atccatggcg gtgttgccgc cgccgagcac gatcacccgc ttggaaatcg
      421 aggtgatgtg gccgaaggcg acattggcca accagtcaat gccaaggtgg atatgcctgg
      481 ctgcttcctt gcggccgggc acgtcgagat cgcggccacg cggggcccct gaaccgacga
      541 aaaccgcatc ccagccctcg gcaagcaggg ccttcaggct gtcgatgcga tggccatggc
      601 ggaactcgac gccgagatcg agcacgtagc cggtctcctc gtcgatgacc cgctcgggca
      661 ggcggaattt cgggatctgg gtgcgcacca tgccgccggc cttcggctcg gcatcgaaca
      721 ccaccacttc atagccaagc ggcgccaggt ctcgcgccac cgtcagcgag gccgggccgg
      781 ccccgacgca ggcgatgcgc ttgccattct tctgctctgc cttcttcggc aggcgggcgc
      841 ggatatcgtc cttgttgtcg gcagcaacgc gcttgagccg gcagatcgcc accgggtcct
      901 tctcgacacg gccgcgccgg caggcgggct cgcaggggcg atcacaggtg cgtccgagaa
      961 ttcccggaaa aacgttcgac tcccagttga tcatgtaggc atcggaatat cgcccggctg
     1021 cgatcagccg gatgtactcc ggcaccggcg tatgggccgg acaggcccat tggcaatcga
     1081 caaccttgtg gaaatactcc gccggattga gatgtgcggc tttcacgaat cctcccgggc
     1141 atgctgtcct ttggctggcc accggatagg caccggttga ccgaatagac ggcaacgaga
     1201 ctccgattaa gccacgacgt caagcacaac agtcaagtgt gctgtcattt cgtcactcat
     1261 ctttggtcgg aagcctggtt tggctgctgg ctggccacct tggccctggc gccggctcag
     1321 tccttgtcgt ggccggaagc caccgatgtg acgctgcttc cgcccgaccc ggagcgcagc
     1381 atgcgatcaa cgagatcgcg cgccgatgcc ggcagagcct cgatttcccg aaccagcaca
     1441 cagcgttcac gcaaggccca ggagtccaca agcgctgccg tggcaatgcg catggtcttt
     1501 gaatgccgga tcgctgccga ttccggcatg atgccgacac caactcccgc ctccaccatg
     1561 cgacacatgg cctcaaacga acggacctga atgcgcaagg ggcggtcatg cccatgctgg
     1621 tccatcaagg cgcgaatgaa ggccagcagc gtcgagccct catgcaggcc gatgtgttca
     1681 taggccagcg tctcggcaaa agcgacgccg ccgcggcctg ccagcggatg cagcagcggg
     1741 gtcgccagca ccagccgatc ggttgaaaag tgcaatgcct gcaaaccttc ggtgggcaca
     1801 cggccggaaa cgatgcccag atcggccgag ccatcgagca cagaagcaac gatatcctgg
     1861 gtcaggcgtt cctgcagatc gacggtgacg ccaggccgct cggccagaaa gcgggccagc
     1921 agttccggca tgaattcagt gaccgccgtg gtgttggcgg cgatccggat gtggccggtt
     1981 gcccctgccg cagcgccgaa ttcatgcttg aggtgttcca cctgcctgag gatcacccga
     2041 gcatggcgca gcagggtctc acccgcatcg gtgagccgca cgccgcgcgc gtcgcgccac
     2101 agcaggcggg tggcaagctg ccgctccagc cccttgatgc gcgcgctcgc tgccggggca
     2161 gacaggaaac tcagccgggc gccgcgtgtc agattgccag cctcggcaac accgacgaac
     2221 aatctgagat cggacagatc gaaatgcatc gcagtctgct tctccgtcag ccttcggttt
     2281 tttggaatgc ttgcttatga aaatacgaat tctcagaacg cggcaagcat ggcatgggaa
     2341 aggcaacgaa ccttagggag ggcacgatgg ccgtcgacat ggcgcttctc aatcaatggc
     2401 tgggcaaaac ggagcggttc actgagcgtc tctcgccgtt tccttccaat gcgcttgccg
     2461 ccacgcttga ccgctgcgac ccgccctatg acgtggggag cgacctgccg ccgctgtggc
     2521 attggctgca cttcctgccc atcagcccgc ttgcccaggc gggcccggac gggcatccgg
     2581 cgcgtggcgg cttcctgccg ccggttcctc tgccacgccg gatgtgggca ggcagccggc
     2641 tgacgttcct caggccgctg cggatcggcg agagcgtcga caagctctcg cgcgtgacca
     2701 agatcgagca caagtcggga cgctccggtg aactggtgtt cgtcaccgtg cgtcatgaat
     2761 tcggcggcgc agccgggccg gcgatcgagg aggagcacga catcgtctat cgcggggcgg
     2821 cggtgccggg agccccggcg ccggctccgg ttccggctcc ggctcactct gaatggcggc
     2881 gaccgatcca tcccgatgcc gtgctgctgt tccgctattc ggcgctgaca ttcaactcac
     2941 accggatcca ctatgatctc gactacgtga ccaaggtgga aggctatccc ggcctgatcg
     3001 tgcatggtcc gctgatcgcc acgctgctgc tcgacgggct caggcgcgcc catcccggca
     3061 agcgggtaac ggcgttctcg ttccgggcga tcgcaccaac gttccatatc gacgagttcg
     3121 ccgtgtgcgg tcagctgacc ggcggcaggg ctcagctctg ggcggacaag gccggcgcgc
     3181 tgaccatgga cgccaccgcc gacatcgcct gacattgccg ccggccggtt tcggccttcc
     3241 cttcgtttca tcattaggac catcagcatg acccgtcccg acgacgccca tcaggatatc
     3301 cgtgacggcg tgcgcgccat ctgcgccgag tttccagccg agtatcaccg caagattgac
     3361 gaggcgcggg gctatccgga agccttcgtc gatgcgctga ccaaggccgg ctggatggcg
     3421 gcgctgatcc cggaagagta tggcggctcc ggcctcgggc tgacggaagc cagcgtcatc
     3481 atggaggaga tcaatcgctc cggcggcaat tccggcgcct gccacggcca gatgtacaat
     3541 atgggcacgc tgatccgtca tggttcggaa gaacagcgcc gccgctatct gccgaagatc
     3601 gcgagcgggg aactgcgcct gcaatcgatg ggcgtgacag agccgacaac cggcaccgat
     3661 acgaccagga tcaagacaac tgcggtgagg aaaggtgacc gctacgtcat caacggccag
     3721 aaggtgtgga tctcgcgcat ccagcattcc gacctgatga tcctgctcgc ccgcaccacg
     3781 ccgctcgacc aggtgaagaa aaagtccgaa ggcatgtcga tcttcctcgt cgacctcgcc
     3841 gaggcgcaga aaaaggggct gaccgtccgc cccatcctca acatggtcaa tcacgagacc
     3901 aacgaactct tcttcgacaa tctcgaaatc cccgccgaaa acctgatcgg cgaggaaggc
     3961 cagggcttca aatacatcct gaccgggctc aatgccgagc gcacgctgat cgcggccgaa
     4021 tgcatcggtg atggctactg gttcatcgac aaggtgagcg cctatgcgaa agagcgcatc
     4081 gtgttcggcc ggccgatcgg ccagaaccag ggcgtgcagt ttccgatcgc cgagagctac
     4141 atcgaggtgg aagccgccaa cctgatgcgt ttcgaggcct gccgcctcta tgacgcacat
     4201 cagccctgcg gcgcgcaggc gaacatggcc aagtatctgg cggccaaggc aagctgggag
     4261 gcggccaacg cctgcatcca gtttcacggc ggcttcggat ttgcctgcga atacgatgtc
     4321 gagcgcaagt tccgcgagac gcggctctat caggtcgcgc cgatctcgac caacctcatc
     4381 ctgtctttcg tcgccgagca tattctcgga ttgccgcgca gcttctgagg tatagtcgca
     4441 cagacaagga ccagtcgcgg gagacgccca tgaacaagcc cgtcacactc acgccggcgc
     4501 aaggcgtgtt catcaacaac cgctggcagc cttcgcgctc cgccaagacg attgcgatga
     4561 tcgccccggc ggagggccgc gcctttaccg agattgccgc aggcaacggc gaggatattg
     4621 atcttgccgt cgccgctgcc cgcaaggcta tcgagctcgg cacctggggc aggttgaccg
     4681 caaccgaacg cggccggctg ctgtcgcgcc tttcccggct gatcgacgac catgccgagg
     4741 aactcgcccg gctggaatcg cgcgacaccg gcaagccgtt gaaacagggg cgggcggata
     4801 tcgtcgcgct cagccgctac tttgaattct acggcggcgc ggccgacaag gttcacggcg
     4861 agaccattcc ctttctcgat ggctatctgg tcaacacgct ctacgagccc ctgggcgtga
     4921 ccggacatat catcccgtgg aactatccgg cgcagatgtt cggccgcacc gttgcggcgg
     4981 ctctggcaat gggcaatgcc accgtgatca agccggcgga agaagcctgt cttgttccct
     5041 tgcgccttgc cgaactcgcc gccgcagccg gctttccgga tggcgccgtc aatgtggtgc
     5101 ccggcctcgg cgaggaggcc ggcgcggcgc tggccgccca tcccggtatc gatttcatct
     5161 cgttcaccgg cagtccggaa gtcggaacgc tggtgcagac ggcggcggcc cgcaatcaca
     5221 tcggctgcac gctggaactc ggcggcaaat cgccgcagat cgtctttgcc gatgctgatc
     5281 tcgacgctgc cgtcgcctcg gtcgctgccg ccatcgtcca gaatgccggc cagacctgct
     5341 cggcgggttc gcgcgttctg atcgagcgct cgctctggga tcgtttcctt gccgatcttg
     5401 cgctgcggtt tggcaagatc cgcgccggaa cgcccgaaat ggatcttgac cttggtccgg
     5461 tgatcagcgc cggccagaaa gcgcggatcg aggcaatgct ggcacgggcg gaggctcagg
     5521 gcgcgaagat tctggcgagc ggccagatcg ccgagaacgt tccgaaggac ggattcttcg
     5581 tcacccccct ggtgtatcac cacgtcgatc cggcttcgga actggtgcag caggaggtgt
     5641 tcgggccggt tctggcggcc atgcctttcg acagcgagga cgaggcgatc cggctggcca
     5701 acggcacggc ttacgggctg gttgccggcg tctggtcgaa agacggcgca cgcgccctgc
     5761 gggttgcccg caaggttcgc gtcggccagg tgttcgtcaa cggctatggc gccggcggcg
     5821 gcatcgaact gcccttcggc ggcatgaaga aatccggcca cggacgcgag aagggcttcg
     5881 aggcgctcta cgaattcggc gcgctcaaga ccatgatcgt caaacacggc tgacgcgggg
     5941 ggcagcgatg gaaaccaccc tctcgattac cggctcgatc gcccggttcg gttccgaccc
     6001 gtcacttgtt ccgaccgaaa gcgcccgcgc cgttgcccgt ctgtcgttga tggattgggc
     6061 ggcggcagcc ttggcggggc tggatgaacc ggtggcgcgg atcacgcggg agcttgcgga
     6121 cgaggaggcc ggcgcggcgc aggcaaccct gttcggtctt gcccggaaag tgccagcccg
     6181 gatggcggcg ctggccaacg gcgcactgtc gcatgcactc gactatgacg atacgcattt
     6241 tgcctatgtc ggacatccga gcgtcgccat ccttccggcg gcgctggcgg tggccgagcg
     6301 acagggcgcc tccgccgccg ccttcctcga tgcggcgctg attggcatgg aggctgcctg
     6361 ccgcatcggg cattggcttg gcacgcagca ctacaatcac gggtttcacc agaccgcgac
     6421 ctccggctgc tttggcgcga cggtggctgc cggccgcctg atggggctcg atactgccgc
     6481 gatgcggcaa gccctcggca tcgcgacgac gcgcgcctcg gggctgaaat ctcagttcgg
     6541 caccatgggc aaaccgttca atgccggcat cgccgccgcc aacggggtcg aggccgcgct
     6601 gctggcagcg cgcggcttcg tgtcccgccc ggacgggctc gaatgcctgc aggggttcgg
     6661 cgaaacccat gccggcgaac ggacggacct ggcgggtgcg ctggcaggtc tcggcaacga
     6721 tttctggttc gagcaggtgc aacacaagtt ccacgcctgc tgccacgggc tgcatgccgg
     6781 gcttgaggcg ctggcagcac tgaaggcagc gcatcacctg caaccgggca gcatcgctgg
     6841 cgtgaccatc accatcaatc cgcgctggct gcgcgtgtgc aacatcgctt caccgacgac
     6901 cggtctcgaa gcaaagttca gctatcggct gaccacggca atggcgcttg ccggcgtcga
     6961 aaccgccgcg cttcagacct attcggatgc gatctgcagc cgacctgacc tgatggcgct
     7021 gcgcgaccgt gtcgtcgtgc ggggtgatgc gtcgctgccg gacacggcgg cgagggtcgc
     7081 tcttcagcat actgatggtc gtgcgcttga cggcgctttc gatcttgacc agccgatgcc
     7141 gctttcccag cggcgcgagc ggattgctgc caaggtgcgg tccctgctgg cgccgcaggc
     7201 ggcggaggcg atcctggcgc tcgacagcgg ccttggccgg cttggcgcaa ttcaggttgc
     7261 cgagaccctg ggccggctgg ccttgtccca cccttgcgcc gctgtcatgt cgccggcgtc
     7321 atccgacagg agttcccatg cgtccgcttg atggcattac cgtcgtcact ctcgaacatg
     7381 cgattgccgc cccgttctgc acgcgccagc ttgccgacct cggcgcccgc gtcatcaaga
     7441 tcgagcgacc gggtgtcggc gatttcgctc gcgcttacga tgcgcgggtc aagggcctgg
     7501 cctcgcactt tgtctggacc aaccggtcca aggagagcct gacgctcgac gtcaagcatc
     7561 ccgccgccgc cggaatcctc gcgaccctgg tcgagaaggc cgatgttctg gtgcagaatc
     7621 tggcgccagg ggcagccgcg cgcctcggct tgtcgtatga tgccgtcagg aagcacaatc
     7681 cccgcatcat cgtctgcaat atctccggct atggcgagga tggaccctac cgcgaccgca
     7741 aggcctatga tctgctgatc cagagcgaag ccggcttcct gtcagtcacc ggcaccccgg
     7801 acgagccggc aaaagccggc tgctccatcg ccgatatcgc cgccggcatg tatgcctata
     7861 ccaacattct cgccgcgctg atccagcggg gacgcaccgg caaaggctgt tcgatcgacg
     7921 tttccatgct tgaaagcatg gtcgagtgga tgaattaccc gatgtattac gccttcgatg
     7981 gcgccagccc gcccgcgcgt gccggtgcag cgcatgcaac catctatcct tatggcccgt
     8041 tcccggccgg cgacggcaag gtagtgatgc tgggactgca gaacgagcgg gaatgggcga
     8101 ttttctgcga caaggtgctg ttgcagccgg agattgccaa agatccgcgc tttacctcca
     8161 acgccaagcg cgcagcaaac cggacggctc tgcgctcgct gatcgtgaaa gccttcgctg
     8221 gcctcacagc cgaggcgctg atcgcccgtc tggatgcggc gggaatcgcc aattcgcgga
     8281 tgaacgacat gcatgaggtg tggcagcatg cccagctcaa ggcgcgcaac cgctggcgcg
     8341 aagtgatgag cccgtccggg ccgatcccgg cgctgctgcc gccgggccgg acgagcagct
     8401 tcgaggcgcg gatggacggg gtgccggctc tgggggagca tacgtcggcg atcctcgccg
     8461 aactcggtct ggacatgacc agaatcaagg ctcttcaggc cgaaaaggct atttgaatgc
     8521 cggtgatcat gaccttttac aaaaggctcg atttctgctt aaagaagcca ccagtcttag
     8581 gaggagacac catgactttc actcgccgta ttgtgatggg tgctgccgca gccgcgatgc
     8641 ttgccggctc gagccttgcc gccctcgccc agtcgccgat cgtcatcaag ttcagccatg
     8701 tggtcgccaa tgacacgccg aagggcaagg gcgcgatcaa gttccaggaa cttgccgaaa
     8761 aatacacggc gggcaaagtg aaggtcgagg tttacccgaa ctcccagctc tacaaggaca
     8821 aggaagagat ggaggcgctg cagctcggcg cggtccagat gctcgccccg tcgctggcca
     8881 agttcgcgcc gctcggcgtc aaggaattcg agattttcga cttcccgctg atcttcaagg
     8941 acaaggaagc gctcggcaag gtcaccaagg gcgccgtcgg caagggcctc ctcgacaagc
     9001 tgcaggacaa gggcatcacc ggcgttgcct attgggacaa cggcttcaag atcttctccg
     9061 ccaacaagcc gatcaagtca ccgacggaca tgctcggcca gaagatccgc atccagtcct
     9121 ccaaggtgct cgacgcacag atccgcgccg tgggtgccat cccgcaggtg cttgccttct
     9181 cggaagtcta tcaggcgctg cagaccggcg tcgtggacgg acaggagaac accccgtcca
     9241 acatgttcac ccagaagatg catgaagtgc agaagcacgc cgtcatgacc aaccatggct
     9301 acatcggcta tgcggtgatc gtgaacaaga agttctggga tggtatcccg gcagatatcc
     9361 gtgcccagct cgacaaggcg atggccgagg caaccgaata tgccaacacc atctccgact
     9421 ccgagaatgc cgatgcactg gccgaaatga agaagacagg aaagatcaat ttctacgagc
     9481 cgaccaaggc cgagatggaa gcctggcaga aggccatgct gacggtgcac aaggacatga
     9541 cctcgcgcgt gggcgccgag atcgtcaagc aggtctatac cgccgttggc ctgccgacca
     9601 actgattgga tgcttcatcc ttcatgatca ggaaaggggc gggtaaccgc ccctttctct
     9661 gtcatagact gcgtcgccag cgtgccggcc cgagcgtatg gactgaggcg ccggttgagt
     9721 cgaccgcgac cgtcaccggc atgtcctcga cgacgaattc gtggatggct tccatgccga
     9781 gatcggcaaa ggccagcacc ctggccgagc ggatcgcctt ggagaccaga taggcggctc
     9841 cacccacggc gatcagatag gcggccttgt ggcgccggat cgactcgatg gcctcggggc
     9901 cgcgctcggc cttgccaatc atcactgcca ggcctgcctg acccagcatc atgtcggtga
     9961 aacgatccat ccgcgtcgag gtggtggggc ctgccggtcc caccgcctcg ccggccacag
    10021 catcgaccgg gccgacgtag tagatcgcgc gtccggcaaa atcgaccggc agcttttcgc
    10081 ccgccgcgag catcctggcc atgcgcgcat gagcggcatc gcggccggtg agcagccggc
    10141 cggaaatcag cagcgtgtcg ccagccttcc agctctgcac ctgagcagaa tcgaggcggt
    10201 caaggtcgac acggcggaca ttgccggccg gaccatcaag cttcagatcc ggccagagcg
    10261 cgagattggg cggcttcaac gccgccggac cggagccatc gaggacgaaa tgcgcatgcc
    10321 ggtcagcggc gcattgcggc acaagcccca ccggcttgga ggcggcatgg gtcggcaggg
    10381 tacggatctt gacgtcaagc accgtggaga ggccgccaag cccctgcgca ccgatgccaa
    10441 gcgcattgat cttgtgaaac aattcgatgc gcaattcctc ggtcttgctg gaggggccgc
    10501 gacgcagcag gtccggcatg tcgatcgggt cgagcaacga ggacttggcc aggatcagcg
    10561 tcttgtccgg gctgccaccg acgccaagcc ccatgatgcc gggcgggcac caacctgccc
    10621 ccatactctc gaccgacttc agcacccagg cgacgatatc gtcactcggc tccagcgcgg
    10681 cgaacttggc cttgttctcc gaaccgccgc ccttggccgc gacatgcacc tccagcctgt
    10741 cgccggccac cacctcgaca tgcagcatgg ccggtgtgtt gtctcgggtg ttcttgcgct
    10801 cgaaaatcgg atcggccacc attgaggcgc gcaagggatt gccgctcacc tgccaggcga
    10861 tgcgggtcgc ctcgtcggcg atttcctgca gcgaacgctg cgttgcaagc cgcacgccga
    10921 cacccagcct gatgaaaatc tgcgccgagc cggtatcctg gcacatcggt cgcttgccca
    10981 gcgccgccat gcgcgaattg atgaggatct gcgccatcgc ctgcttggct gccgggctct
    11041 cctcgcgctc ataggcggtg accagattgg tgatgtagtc ctcaggatgg tagtaggaaa
    11101 tatactgcaa ggcatcggca agactctgga taagatccgc ctctgtgatt gtggtcatgg
    11161 gtgctccccg gtcctggatc acgctctcgt tttgattgaa tcatcaaaac gagagcgtga
    11221 tcgataatag caactttgag cgtgccttcg caaaaaaccg ttcacacctt ttcgcagcat
    11281 gctctggagt gggcggtggc ttgattgtgc ggtgcgatga ccaccttgcc ggtattggcc
    11341 ccgctgtcgt ggcggtcaag cgcggcatga atgtcatcaa gcgcatagac gcgatcaatc
    11401 accgggcgaa tgtcgtgacg ctcgcagaag gtgatcagat cggcaaactc cgcaaggctg
    11461 ccaagggttg agccgaacac ctgcaactga cggatgaaca gccgctggat atcggccgat
    11521 gggtggctgc cggacgtggc gccgcaggtg accagccggc cgccgcgcac cagcgccctc
    11581 agcgcctgag gccagaccgc ctcaccgatc gtctcgatca cgacatcaac gccgcggccg
    11641 ccggtcaggt ccatcacgcg cctgacgaca tcctcgcggc cgccgtcgat ccctgccaat
    11701 gcgccgaaac gagcggcaag atcgagcttg gtcggatcac gcgaggtgac gatggcgcgt
    11761 cctcccatgg cctggataag ctgcagcgcc gccaaggcca cgccgccgcc gatgccgaaa
    11821 atcagcgccg tctcgtgcgg ctgcatccgc gccttgccgg tgatcatccg ccacgccgtc
    11881 aggtgggtga tacccagcgc cgcgccttcg gcaaaactga acatgtcggg caagggaaag
    11941 acattctccg ccggcaggca gacgaacccg gcgaaggttc cgtcgcgatg ctcgccgaga
    12001 taacgggcac ccaggcacag gggtcggtcg ccgcgccggc agaattcgca tcgcccacag
    12061 gtcactccgg cataaagcgt gacacgttgc cccggctgca agccgctctc accggcagga
    12121 accgagataa tcgtgccggc gccgtcaacc cccatgatct gcggcaggct atgggtaatc
    12181 ccggcgcccg aattgcgcat gtagagatcg acgcggttga cggttgccgc ctcgacgcgc
    12241 accagcacct cacccggccc cggcaccgga tcaggccggt cgccagcagt tacaacctcg
    12301 ttgccgccat gtccggtcag aaaggccgcg cgcatcagtt cacctgcact gggggtcggc
    12361 ttcaaaacgt tccaaccgga ccggacaaca acctaatccg cccatgcccg cgcgagcaag
    12421 cgcccgtcac gctcccgcat ccagcgctcg gatgataccg cgcagttctg ccagaccctt
    12481 cagccggccg atggcggtat agccgggcgt cacccgttcc gccttgatgt cattgagcat
    12541 gcggtggcca tgatcggggc ggaaaacgat gctttcagag gacgcgcgtc gccggtcctc
    12601 ggccagaagt tcgcgcaaga ccgaaaccat gtcgacgtcg ccatcaagat gatcggattc
    12661 gtggaaggac aggccatcgg cttcacgccg tgtggcgcgc agatgggcaa aatggatgcg
    12721 cgaggcaaag cgtcgggcca tcgccggcag atcattgtcg gcgcgcacgc ccagcgatcc
    12781 ggtgcagaag cacaggccgt tggccgggct gggcactgca tcaaacaggg cggcgaaatc
    12841 ggattgctga gaggcaactc gcggcaggcc aaacagcggg cgtggcggat cgtccggatg
    12901 aatggtcagc cgcacaccca attcctcagc acgcggagta acggcgccaa gaaactcgat
    12961 gagatggcgg cgcaacaccg ccgcatcgat cctctcatac tgcttcagct tgtcacgaaa
    13021 catcgggatc gtcatcggct cggtggtcga gcctggcagc gccgaggcaa tcaccatcac
    13081 gatgtaatcg acatcggcct ggctcatcgc ggagaaaacc ttgcgggcct tgaccctgac
    13141 atcttcgctg taatcccgtt ccgcgcctgg acgctgaaga atgaaaagtt cgaacgcagc
    13201 gaatttcaca tgatcgaacc gcagcgcggt cgcaccggtg ggcatgacgt agtcaagctc
    13261 ggtgcgggtc cagtccacca ccggcatgaa attgtagcag atgatgcgca ggccctcggc
    13321 ggcggcggcc tccatgctgg caatccaggc ggcgatggag cgcgttgcag cagcacccag
    13381 acgcttcaca tcatcgggaa cagggatcga ctcgatcacc gaccaggcca gcggcgttcg
    13441 ccccgcaggc gttgtctcaa tcagcgcctt gtgtcgcgac acctcgctgc gtgtccatcc
    13501 ttgtccgatc ggcagatgat gcagcgagga aacgacatca ctcgcaccgg cctgacggat
    13561 gtcgtcgaac gagaccggat cgttcggtcc aaaccagcgc cagccctgcc gcattgtccg
    13621 ttctctccaa ccattccggc atcggggtgc tcgccggcaa aatgcgatgc ttctaaaata
    13681 ctagtatatc agagacctgg tcggatcaag aaagcataga gaacgcggcc gtcattcacc
    13741 gtgggcaggc gccggctcac agactacggc ccaatccttc ggcaagaccg acctggggtt
    13801 caaatcccaa tacctgccgc gcagccgcca tgtcgtagcg agcctttcgc atgaacaggg
    13861 cgacttcgcc cggcatgggc gccgaagcgg ctgcggccat tcctggcatc ccgagctttc
    13921 gccagacctt ggccggaacc gccagagcct gacgccagcg aagcgccggc gctgcaatgg
    13981 gctggagcgg tggcgccccg atggcagccg ccagactgct gaaataatcg ttccaactcg
    14041 gcgtttcggg gccgatggca ttcagcatac tgatcgattg ccccgattct ccctccagtt
    14101 cctgcacggc ggaaaggcac atggcagcca gatcatcgac atggatcaga gcggcgatcc
    14161 cctcgccggc agcaccgaac gtgccccagc cgccaaggcg gatacgttca accatccgat
    14221 caatccagaa tgggctgttt ttgccgtaga cgatgccagg ccggagaatg attgcccggt
    14281 tgcccgcctg ctccgtccag tggccgacca tggtttcgca ggcaagcttg ccggctgcat
    14341 agccatcaat cggcggcggc agggtcgcgt gttcgtcgat cgggccttcg agattgccat
    14401 aaacggcgac cgaactgagc aacaccagcc tgcatgcagc cgcgtgcgtc attgcagcga
    14461 gcagggcgcc gcattggtcg gccatgcgga ccacatcgcc ataggcagcg tgcaccacca
    14521 gatcgacatc ccgcagagcg gcggccagcg tttccggccg gtcgagatcc gcgatcaggc
    14581 agcccgcctg agccggaggc cgccgacgga caccggcgcg cgtctcatgg cccgcgtgcc
    14641 gcaaccggtc gacaatgacc ttgccgatga agccacccgc tcccgtcacc agaatacggg
    14701 cggcgcgatg ttgcggcatg ctcaggtcct ctcaagaaac ccgggaacag gtattcctgc
    14761 cagacgggcc atctcggcga ggcgggagcg tgcaaaagcc acgagcagca aggcatagac
    14821 cgcgacaccg agcaaaacag cgattgccag tgacaggacg tgcgaagccg ccgcagccgg
    14881 cgcagccagc aggatggtga caagacccat cagcgccgac agcagccagg cgtgggccag
    14941 cgcatcaagg cgggccagcc gcgtccagcc aagatgggca gcgggaagga cgacggcaca
    15001 gagcgcgcgc agtccatctc cggccaagcg cgacaggccg accgagatcg gtccaagggg
    15061 agcgcaaacc acgagaatga tcacactgct gatcaatcct agcacaagcg aagccagact
    15121 gtgttccggt cgtccggcgg ccgaaaaggc cgtatagacc agcgcggatg gcactgccag
    15181 ggcactggcc agcgacaggc atatcgccag cgcgatcgcc ggctcccagc cagggcccgc
    15241 catcatcggg atgagatccg gcgctacgac tgctagcccg acaaacgccg gaaccgtcac
    15301 caacgctgcc ttggccacga catcgcgggc gcgctcccgc aagcggtggc ggtccggact
    15361 gcgggcaaaa taggaaaagg cgagattgtg cccagtggcg agaatggcgc cgcggatcgg
    15421 ttcaatgagc cgcatcgcca tgttgacatg gccaagaacc tgcgtgccgt agatcacctg
    15481 aacgatcgca ttgaaggcaa gatagccgag attctccgtc agtcgcgaca gaaacgacag
    15541 gcccgcaaac cggcccaacc ctctgagatc cgccggcgac cagagcggca ggatcagata
    15601 gccgagggca cgctgaagca ggatggcgcg cgcaagcaca accagaaccc gttggccaat
    15661 cagcgcccag atgccaaagc cggcgaaagc caggccaagc gccaggcaaa tgccgatgac
    15721 atggcccatc aatgttgcat aggcgacatc attgaaacgc ttcaggcgtc gggccagcgc
    15781 cgtcatgatg tccgaatgag cggagaagac gatcgagacc atcgtcaccg gcagcaagag
    15841 agcgatctcc ggttcgccga aaaccgtggc caaccatcgt cccgccaggc cggaagcggc
    15901 gatcaacaca attgccgcga cccaggacac accaagcgca gcccgcaaat gccggcgtcg
    15961 caagcctcgc tggcgggtca acgcctcctc gaatggcgcg ccgacgaaca gcgaggccgc
    16021 cgcaacaatc gccagaacca gcgcggaaac cccgaattca tgcgcgccca gcaaccgcga
    16081 gaaaaccact gtgctgccgg tcaacagggc cagccggccc aatgcgtcaa gcccggccca
    16141 gccgagccat ccgatgagcc gcgagccggt cgctccctgt ctgcgcagac tctcagccat
    16201 agcgataccg cgcaaaggct tcggcgcgca tctgcagcgc ataggccgga ttggcggaat
    16261 tgccaccgag ctgatgcggc ggatggcgca tcgattggac cagcgcccaa atcgccaccc
    16321 cgaccacggc cagcacgatg ccaaggaccc agcccagaag ggcaaaactc aacagtttcg
    16381 ggagtgggtt ctgcggcagc ggagagcgcg caaccgaaac gatccgcagg ttggccgagt
    16441 caagcttgcc gtattcccgc gcctcgcgca tgcgcgtttc gaaactttcg agcaccttgc
    16501 gattggcctc aagcgattgc tccaactcgc gcaacgcgat ttccgactgc ttggcggtcg
    16561 caaccgccac cgtcagttcg ctgacggtct tgctgaggtt gccctgttcg gcttcgctgc
    16621 gggacagatc ggagcgggcg gcaactcgga tggcgctcat ctcgtgcgaa atcgcgcggt
    16681 cgatttcggc cacgcggctc cgtgcgtcga tcagcgccgg atgtcgtggc ccggcccgcg
    16741 cgaacaatgg cgccagctcc gccatcaccg cacttcgccg ttcatagagc gacagaagct
    16801 gcctggtatc ggcctcggcg ccgagcgccc ccagggcgct ggggttgcgc gcggccgttt
    16861 cgatctggcg catccgcgca cgcgcgcggt cgatacgggt ctgcgattcg ttgagggcgg
    16921 cggtggcggt cgccaggctg gtttcgacaa tcagacgatc gccgacgcgc aaaagattct
    16981 tgtcgcgacg atagtgatcc agccggtctt ccgaatcctt gacggccttg cgcagatcgc
    17041 tgagccgacc cgacagatcg ccggtcaacc ggttgatcgt ctcggcccgc gtggcggcct
    17101 cttcctgaat gtaggcattc acaaccgccg aagcgagact ggttgccagc tgcggcgttg
    17161 ccccggtcgc ggtgacatca accacatagc tgcgctcgac acgcttgacg gtcaggacgc
    17221 gacgcatttc ctgcaaaccg cgctgccagt cgccgtccca accctcgcgg gggcagaagc
    17281 gcgtaaagcc atggcccgat gccttgtaga cggagtgggt tgcggtcttg gctaactcac
    17341 gaagctcgtc gcattccgca cccatgacgc gcgaaagcac gcgctcggac tggatgaccc
    17401 ccatctggct ttcaacatag ctgatcgcgg cattcgcatc atgatatccg ctggtcagat
    17461 cgttgttgaa taccttcagg ccgcgcggat cgaacagcaa ttgtgccgtc gccggatagc
    17521 gaaccggact gtagtggcga taggcagccg ccatgccggc gaagatcagg ccactgagaa
    17581 aaaccgccca gaaccaccgc tttagaccct gaccaaaaag cgcaaaagtc gaccgccatt
    17641 cctggacggt tgccgaaacg gcagccccag gccttagact ggacgggatt tgcggtgtca
    17701 tccggttgag aatccgaagg ttttcggtcc caaactagcg agccggagaa agctaaactt
    17761 taaacgatcc cagcaatcag atgctatggt tagccaatca ttaccggagt ttccggcccc
    17821 gtcactccgt caggggcgcg gctcctttag gcaaccctga ccgctgacct catccggcat
    17881 tgcggtaggc cttgcgggag aacaaggtca ggaaaagaat ccagacatcc agcacgaacg
    17941 accagtgatc gatgtagtaa agatcatggt ggacccgctg cgagattttt tcaggcgtgt
    18001 ccgtttcacc gcgcagaccg ttgacctggg cccagccggt aatccccggc ttgacgttat
    18061 ggcggcgcgc atagagcacg atgttctgct cgaaagcatc gtcatgcacc agcgcatgcg
    18121 gacgcgggcc aaccagcgac atctgtccca tcaacacgtt caggagctgc ggcagttcat
    18181 cgagattgta gcggcgcagc cagcgaccga ctcgggtgat gcgcggatcg tccgccgtcg
    18241 cctgcgttac aacgggccca tcctcggcaa ccttcatgga gcgaaacttg agaatgcgga
    18301 acggctcctt gttgaagcca tggcgcatct gacggaacag gaccggaccc ggactgtcga
    18361 gccggatcgc gaccgcaatc gccagaaaca ccggcgcaag catgatcagg gccaatgccg
    18421 aaaggatgat gtcgctgccg cgcttgagca ccgagccgag cgtgccgatc gcatagccgc
    18481 gcaggcggaa gccggaaatc gccccgttgg cgcccatgtt ggcacccccg aagcggttga
    18541 gtggcgagtc agggtccagg tgcagatgga tcgaagccgg aacccgcaga aaggccgtca
    18601 cgcaggcttc aatagtgtcc gtccggttcc acggaaccgc aatcacgatg tcatccggca
    18661 tcagccggcg gatcatcgtg gtggcttcgg caagatcggt gtcgcggatc gcccggtcct
    18721 cggcaatggt gtggcgggcg accaccagca tcccctgctg ttcgggttgc tgcatggaga
    18781 tgaaatcgtc gatatccttg gcaaatccga caatcacaac ccgcgctgcg agggcgctgc
    18841 cggccaccgc attgcgcttc atcagatgca ccagtccggc gcgccccaga aacagcgcgc
    18901 acagaccggc cacatagaaa acgaggaacg tgccgcgcga ggtatcctcg atcacacggg
    18961 caagaaagcc gaaggttgcc gcggcgagaa aggcaaggtt ccattggctg aacacctgcc
    19021 gcgcatgacc ggacagatca aggtaatcgg ccagggaata gctgttccgc agcaggtttg
    19081 agccgacaaa cagaatggcg gcaaacaacc cgatctggat attggcaaca catacgctgc
    19141 cggcatcgtg gacaatctcg ttataggcaa agtccgaagc caacatcgcc gcaagaatgg
    19201 tcgcggcatc caccagcatt gcaaccagcg aaaaacacag gcgggtgaac aatacagcca
    19261 cgcgaccaga gccggcttcc gcctgcacca cgtcgcgctg ccgtaaagga atggcagacc
    19321 ctgtctctct gcatagcggc tccgcaagcc gcgacgcccc gcaaacgaat tcagtcataa
    19381 cggccccctg tcttcgcgcc ggtcgtcgag cgacattctt tccgcgatga cagggaccac
    19441 cttagcgatg gcgaataaac ggagccctaa ataacaggct gaatcggccg tttaccacgg
    19501 caactctagg caaagtaaac aataaatcac tcgaaatcca ttgtttacgt tacgaaagcc
    19561 acggcgttca caagccatga agtgtttatt ggtcatttaa ctaaaataca cagcaattct
    19621 gtgttagtgt tcatattttg ctttcatatt gctggaattg aaatgagtgt ctcgcctgta
    19681 accaggccac tctcaagaaa gcgaacgaac gaagcgggcc agccgctgct tgttccgcag
    19741 gccaagcagc gaggccaggg cgacatgccc ggggctgcca tgttgcccgg acagacctag
    19801 atcgcgcgcc gcagcctcgg cgatggcgcc aagatcgtcc atgccgttgt tgtaaccgag
    19861 gcgcgcaagt gcgtagtagg caagggcaag ctcgcgctgg gggtcgaaag gcagctcctc
    19921 cagacggatg cggatcttgt cgagcgcacg gatctgggat tgcagggtgc gcggcgtctg
    19981 tttgcgggtg atgcgtccag ggttgtcatc ctgaacatag atgccctgcc ctttcggcgc
    20041 cgtcgtgatg cgcactccct gcatgagggc gcgatggatg aggtcgccgt cctgattctt
    20101 ggccagcgtt tcgtcccagc ctccgagggt ccggacataa tcggttcgcc agatgaccga
    20161 acagggcggc gtgtaaccgc cgctgagcca gcgcttcagc accgtgccgc aggacagatc
    20221 ctcgtagcgc gtggccggat tgtgcgcatc acgacggcca tccggatatt cgaaggaaaa
    20281 actgccaagc accacatcgg ctgcatcctt gccggctgcc tcggccgccg aggcaagaag
    20341 ctgaccttcg atgtaatcat cagcatccag aaacatcaca tagggcgaac tgaccaggcg
    20401 caagccggtg ttccggcaca tcggcgcgcc gcttccggcg ggcagaacga cgaccttagc
    20461 tcccggaatc gtcgtgcgag ctacatcctc caacggggca tcttcaccgt ccagaacgac
    20521 tatcaggtcc ggcgggcgaa cggcttcccg gcagctcagc agcgtgcggg caagagtgcc
    20581 tgttgcgcga taggcaggga caacgatgct gacagacatg cccgacagac cttcatggtt
    20641 cccgggcgcc ggaaacgaat ggcagcagtt gatccgaggc gcgcccttgc ctggacaacg
    20701 ccactcaacc cgacaaccaa taaaggtcag cccggatttt ccgctgcatt ttagtcaaca
    20761 ccggctgtcg ctgcggaatg gccaattatg tttagggaat tgcaacggca ggtcgctacc
    20821 gtcccttgcg tcagcctgag gggcaattct gcgtcatggc cgcatcagat atcacttcga
    20881 tcgcattcgt cggttgcggc tacgtcgccg attcctatcg ccattgcctg ccgctgcatc
    20941 ggcaaagcct gcagcttgcc ggcgtctatg atcgcgatcc ggagcgtctt gccgcgttcc
    21001 gcgccacctg gaacgatcat gcctatgaca gcctggaggc gttgcttgct gatccagcgg
    21061 tcagcctcat tgtcaacctg acgccaccgg aagcccatgc agaggtcacg ctggcggctc
    21121 tggccgccgg caaacacgtc tatactgaaa aaccgctggc tatgaccacg gccgaggcaa
    21181 tccgattgcg cgatgcggcc ataaaggccg ggctcaggat cggcgccgct ccctgcaatc
    21241 tcttgggcga agcctcccag accctgtgga aggccgtgcg cgaagggcgc atcggcagac
    21301 cggttctggc ctatgcagaa cttgatgacg ggatggtgca tcgcgccaac taccgccagt
    21361 ggatcagccg gagtggcaga ccctggcccg cccacggcga attcgagacc ggctgcacct
    21421 ttgaacatgc cggatatgcg atcacagtcc tggccgggct gttcggcccg gtgcggcgtg
    21481 tcaccgcctt ctcggccctg ctcatcgcca acaaggatac cgaccagccg ctcgatcatc
    21541 cagcgccgga tttctcggtc ggcatgctcg aattcgatgg cggcgtggtg gctcgcatca
    21601 ccaattcggt ggttgctccc tatgatcaca ggctgcggat catcggcgag agcggcacgc
    21661 tcgaaatcgc cgaaccatgg gactatgcct ccccggtgcg tctgaaaaag ccagcggaaa
    21721 cccgcctggc ccgcctgctc gaacgacgct tcggcagagc actcggcggc gagaccatag
    21781 caccggcgcg gcccgtgccc ttcagcggcg ggcgtggcag accgaccatg gattttctgc
    21841 gcggggtggc agagctggcc caatccattc gcgaggggcg ccgcagccgg cttgatgagg
    21901 actttgccgt gcatgtaacg gaagtcacgg aaatccttca gcaccctgat cggttttcca
    21961 ggccacaggt ggtgcaatcc agtttcaagc caatcatgcc gatggactgg gcggattgat
    22021 catgtccgat aaaccctatt cggccggcat cgtcacctgc tcctacagcg gcgattacga
    22081 tgcgtgccgc atcctgtgcg agagcatcga tcgcttcgtg ccggcagcga tggcccatac
    22141 gctgtatgtt ccgcgcgccg atgtaccgat gtttgcgcat ctgggaaacg cccgtcgccg
    22201 catcgcgaca caggaggaac tgctgcccgg ctggttctgg aagctgccgt tgccttcgcc
    22261 ggaatggcgc gcgcggctgc tgctgccgcg ccgcaacgtc tatctcaccc cgttcagcct
    22321 gccggtacgc ggctggatcg cccagcaggt gatgaaactg gccgcaacgc agcgcgccga
    22381 gaccgacatc gtcgtcaaca tcgacagcga caacgccttt atccgcccgt tgacgatgga
    22441 tcatctggtc agggacggcc gcgtccggtt ctatcgcaat ccggacaagg tgccgcttga
    22501 gtcgcaccgg cgctggcatc gggcggcagg caagctgctg ggcctgccgg acagcgactt
    22561 ctacggtgcc gagtatatcc atccgctggt ggtctggcga cgcgatgtgc tccgggcgat
    22621 gctcgaccgg atcgtcgagg tcaccggcat ggattggcag aaggcattgg cacgcaccac
    22681 ccattttgcc gaattcgtcc tttacggggt ctatgccgac aaggtggccg gccttgacca
    22741 ggccggcctg tgggcgagcg accagccatt ctgcctgtcg cgctgggccg aggatttcag
    22801 aagcgcggcc gacgaggtcg ccttcgtcaa cgagctacag ccccgtcatg tcacctgcct
    22861 gatccagtcg accattccaa tgccgccgaa cgaacggcag cggattttca acctggccac
    22921 cgatgccgcc gcagcgatgg accgggccgg ctgaccgagc cgacgacgac tgcggttgac
    22981 actgtgcctt ccagcccgtt ggatgcctgc acgctcatcc ggacgccctg caatgaccct
    23041 ctacggctac tttcgttcat cggcagccta tcgctgccgc atcgccttca acctcaaggg
    23101 actgaaaccc aacctcgtct ccgtgcatct gcgcaagggc gaacagcggg cagacgattt
    23161 tctcaagcgc aatccgcaag gcttcgttcc ggcgctggaa accgcgggcc aggtgctgac
    23221 ccagtcgctg gcgatcatcg aatggcttga ggaaacccat cccgagccgg ccctgttgcc
    23281 cagggatccg tttgaccgcg cgctggtgcg ggctttggca ctgaccattg cctgcgacat
    23341 ccaccccttg cagaacttgc gcgtgctcac ccatttgcgc aaccatctcg gacaggatca
    23401 ggccgggatt gatgcctggt gccgcacctg gatcgagagc gggcttggcg cctacgaacg
    23461 gatgctggtg gacaagggct gttctggcgg cttttccttc ggcgccacgc cgacgctggc
    23521 ggatgcctgc ctcattccgc aggtgttttc agccgaacgc ttcggcgccg atctgaccgc
    23581 catgccgacg atccgggcca tcgccgatca cgcggggcaa cacccggcct ttgccgccgc
    23641 ccaccctgcc cggcagccgg actatgaggc ttgaccgggc ggacgtctcg ggccgcttgc
    23701 gtgatgacgg gttgaatgcg atgaaccaca acaggaacag gcattccgac gacgggctga
    23761 cggtggcgag gaaccggcaa gccaggttgt catcggatgc gcgtgatcgc cgatctaaaa
    23821 acgggagagg gaccctatga cgttcaaccg ccgccacttt tcggccgctc tggccgccgc
    23881 cgttgctgct ccggccgtgt tctccggcgc acgcgcgcaa gcccaggtga ccctgcggct
    23941 gcaccacttc ctgccgcccg tgtccaacgg tcaggccaag ctgctgggcc catggtcaaa
    24001 gaagatcgaa gcggaaagcc agggccgcat caagatcgac atctatcctt cgatgcagct
    24061 cggaggaacg cctccgcaac tgttcgatca ggcgcgcgac ggggtcgtcg atatcgtctg
    24121 gacgttgccg ggcaatacgc cgggccgttt cccgacaaca gaggtgttcg aactgccgtt
    24181 catctgcgcc gaaaagggca tcgtcaacgc caaggccagc caggaattcg ccaacagctt
    24241 cctcaaggat gaggtgaagg acgtcaagct tctggcctat tggctgcacg atgccgggca
    24301 tatccatgcc aacaagcagg tgcgcaccat ggatgacctc aagggcctta agctgcgcaa
    24361 tccgacgcgg ctggccggcg aggcgctcaa ggcgctcggt gcaacatcgg tcggcatgcc
    24421 ggtgccgcag gtgcccgaga gcctggccca gcgggtgatt gatggcgcgg tcattccgtg
    24481 ggaggtggtg ccggcggtca aggtgcatga actggtcaag ttccacaccg agatcaccgg
    24541 gtcgccgacg ctctattccg ccagtttctt cctggcgatg aacaaggcca ggttcgacaa
    24601 cctgcctgat gatctcaaag cggtcatcga cgccaattcc ggcatggcct ttgccgatat
    24661 ggccggcaag atgtgggatg aggaagcggt gcgcgtgcgc gacatgatcg ccaagcgcgg
    24721 caacaccatc acccagctcg ccgaggatga gaagcagcgc tggatcaagg cgacggagcc
    24781 ggtctacggc gcctggtatg agcagatgaa ggccagatcg ctggacgggc cgaagatggt
    24841 cgaggcggcg cgggcgctgg tcgccaagca cgcgaaagcc tgaacgcggg catattccgc
    24901 acatccggca gcgcggccgg cagggagtag gcagtatggc tgaccaggag ccgccagcgt
    24961 ccgggcgcga caagcggctg gaacggatca caggctggct ggcgacattc ggcggcctgc
    25021 tggcgctggc tgtcgccatg ctcgtggcgg tcagcgtcct cggtcgctgg ctgttcagtg
    25081 caccgatcga cggcgatttc gaattcgtca agatggcgac cgccgtcgcg gtgttctcct
    25141 atttgccgta tacgcaagcg cgctgcggca acatcatggt cgataccttc accacatcgc
    25201 tgtcgccgcg gatcaaccgg cgcctcgatg ccttctggga ttttgtctat gcgctgtttg
    25261 ttggcggcat ggcggtggcg ctcgcaatgg gcacactcga tgccctgcgc tcgggcgaga
    25321 cgaccatgca gcggcaaatc ctcctctggc ccagcattgg tgcaaccgcc gtgttatgcc
    25381 tcgtgctggc aatgacggcg ctgacaaccg gcatggcatt gttgaaggat cggcaggatc
    25441 gcggttgatg tcgggcacag gcgtcgcatt tatcggcttt ggcgtcatgc tggtgatgct
    25501 ggcattgcgc gcgccggtcg gccttgcgat gctggtcgtc ggtgctgccg gctacatcca
    25561 tctgtccagc gcgcaggcgt tcttcgccta tatgaagacc aatgcctatc atcagttcgc
    25621 caattatacg ctgtcggtca ttccgctgtt catcctgatg ggcgcgctgg ccgagcgggc
    25681 cggcattgcg gcgagcctgt tcaaggcggc tgaacgcggc tttggccgca tgcggggcgg
    25741 tctggccatg gccgtgatcg gcgcctgcac ggcgttcggc gcgatctgcg gctcatcggt
    25801 ggccacgacg gcaaccttcg gccgtgcggc cctgcctgaa ttgctcaagg cccgctacag
    25861 cggcgggctg gcgacagcca ccatcgccat gggcggcacg ctgggcattc tcattccgcc
    25921 atcggtcatc ctggtcgtct atgcgatcac caccgaacag aatatcgcca agctgttcca
    25981 ggcggcgctg atccccggcc ttctggctgc gctgttctat tgcatgacga tcgccattgt
    26041 tgcgcgcctc aggccggagc aggccccgga agggatcccg tcagagctcg aaccggcctc
    26101 ccatcccgcc tggatccgcc tcttggctgc agcactgctg ctggcggcgg gggcggccta
    26161 cctttcaggc catcttggcc tgtggtccgc tgccggattg gccggtcttg ggttggcaat
    26221 tgccatcgtc gatgctgcga tcatgcccgc catgaccatc gccatcatcg tcgtcggcgg
    26281 catttatggt ggcgtgttca cgccaaccga aggcgcggca gtgggtgcga ttgcgatgct
    26341 gggcgtcggg cttgccaacc gttcgctgag tgccggcgac attctcctgg cggtgcgcca
    26401 gactgctgaa acctcgggca tgatcttcat catcctgctc ggcgccgaga tcttcggagc
    26461 ctttctggct ctgtcgcgcc tgcccaccca ggcagccgag atgatcggct cgtccggcct
    26521 gtcgccaacg ctggtgctgg cgggcatgat ggtgttctac atcctgctcg gcgcggtgat
    26581 ggacgaactc gcgatgatcc ttctgacgct gccggtattc tttccgatcg tccaggcgct
    26641 tgatttcggc atgctgccgg acgaagtggc aatctggttt ggcattctgg tgctgatcgt
    26701 cgtcggtatc ggcctgacca tgccgccgat cggcctcaat gtctttgtca tatcgtcgct
    26761 tgcccgaacc gtgccgatca aggcgatcta catcggcgtg atgccgtttc ttgtcgccga
    26821 tttcattcgc ctcggactgg ttctcgcctt tccgagcctt acgctctggc tggtgcggct
    26881 gctgcaatag ggcgcctttt gcgggcgccc ggtctcagtc cagaccggtc attgcatggt
    26941 gcagatggcc gacaagatag ggcaggaatg agcgccatat ctcgatatga aagcggtgtt
    27001 cggtctgccg ccagagcagg atttcggtct tgtggaagac actgcgtgtc gctcgcccgg
    27061 ccgggaagga tttcaaccgc agatccagcg gacagccagc ggcgatcagg cgcgccgcca
    27121 gcgggccttc gaggcacaag cccatctgcc ggtgtgtgac atccaccaac gcatgtggcg
    27181 agccggccag cgccttgccc aactcctcgc caagcatcgc cgcctcggtt gccggggcca
    27241 gcagcagcca ttcgtccggg ccgagccaca gggcggcgcg cgacccgtcg gcgccggtca
    27301 tcacctccag ggggttcacc ggcaaggcca caccgaaggc cctgccggcc atcgtccgcg
    27361 ccgcatccgg accttgcagg acaaaccgcg accgctcctc cgccatacga accgtgcagg
    27421 ccggcgaaac cggcaggatc aaccctgcgg cggcgccgga ttgcgtcggg cttttcccgg
    27481 gtgcaccctc aaccatggag ccggctccct tcgggatcga caaagaccgg cgctgtcacc
    27541 cgcaccggca cggaccccga cagggtcgag gcgaacaggc cctcgccctg ccgcgctcgt
    27601 ccctcggcca gcagcgccag cgcaatcgag cggccgaggg cagcgctcca ataggacgag
    27661 gtgacatgac cgagtgcagc tgaacccgga gcgggatggg cgctcgcggt cagttgcgct
    27721 ccctcatcca gtacgaccgc aggattgtca gtcagcaacc cgaccagttg cttgcggccg
    27781 caagcaacca gatcggggcg cttcaacgaa cggcggccga tgaactcgcc tttcgacttg
    27841 cccaccgccc atgccagacc gagatcctgc ggtgtcacgg tgccgtcggt ttcctggccg
    27901 acgatgatat agcccttctc ggcgcgcaga acgtgcatgg cctcggtgcc ataggtaacc
    27961 gcccgatgcc cttcacccgc ctggcgcaga acctgccaca gcgccctgcc atagccggcg
    28021 gggacatgca cctcgaagcc caactcgccg gtaaagctta cgcgcaggac acgcgccggg
    28081 atgccggcaa cctgcccctc ctggatcgcc atatgcggca tcgccgcggc cgcgatgtca
    28141 aagccctgga tcagtggcgc cagaagatcc cgggcacgag gtccttgcac cgccaccact
    28201 gcccattgct cggtcaccga cgtcagccat acgtcgaggt cgggccattc ggtctgccga
    28261 taatcctcca tgtggtgaag cacgcgggcc gcaccaccgg tcgtggttgt caggtagaac
    28321 cggttcacgt cgaggcgggc gaaaacgcca tcatccatca ggaacccggt ctcgcccagc
    28381 atcaggccat agcgacagcg gccgggtcgg agcgaagcca ccggcgtgca atagatgcgc
    28441 tcaagaaacg tggcggcatc cggcccgcaa acctcgatct tgcccaatgt ggaggcatcg
    28501 aatagcccca ctgcctgccg caccgccctg cattcccggg caaccgcagc gtggagatcc
    28561 tcattgtgct gaggaaaata ccgggcgcgc ttccactggc cgacatcctc gaacacggca
    28621 ccggccgcag cggccagatc atgcatgggc gtgtgccgga ccggctcgaa caatgcatcg
    28681 cgatgggcgc cggcaagagc cccgaaggcg accggcgtat agggttggcg aaatgtagtc
    28741 aggccgacgt cgggcagaga gcgtccgagc gctctggcgg cgatcgccag ggcattgagg
    28801 ttggaggtct tgccctggtc ggtcgccatt ccggtggtgg tatagcgttt gacatgctcg
    28861 atggagcgat acccctcacg cacggccagt tcgatatcgg cggccttcac atcgttctgg
    28921 aaatcgacaa aggcctcgcc ggagtccgta tcgcccggct gcagaccgag ccagccgcct
    28981 gtctgcggcg ggtctccggt caccgcaaaa tgccgcccgg gcgcctgccc agtagaggca
    29041 catccggcgg catgtccttc ggccagcgct tcagcgaggc cgaaaacgcc gttgcaggcg
    29101 cccaccgaga caatcccgct gtttccgcca cccggcagga atagctgccg ttcagcgtcc
    29161 catcttggcg tctgccgcgc ctgcgaggac aggtgaaggc tgggagtcca gcctgcactc
    29221 atcagcacgg cgtcacagtc cagttccatc gcctggccca cacgctcgtc agcgcccgca
    29281 cggatcaaca cgccagaaac ccggcggcgg ccacgggttc cgtcaacaat ggcctcttcg
    29341 atgaccttga ggccggccgc tcgcgcctcg gcgggcaaag ccccccgcgc cgccgggcgc
    29401 agatcacaga tggccgccac cgtaatcccc gcctgatgca gatcgagggc agcgcgatag
    29461 gcactgtcat gggcggtgac cacgacgatc cggctgccga ccctgacccc atagcgattg
    29521 agatagatgc gcgccgcatc ggcgagaatg atgccgggtc gatcattgcc agaaaatacc
    29581 agcggccgct cgatggcgcc ggtcgccaga accacgcgcc ccgcccgcac ctgccaaagg
    29641 cgctggcttg gcctttcacc cggcgcgggc ccggaatcgt ccatacgttc aacaagaccg
    29701 acgagattgt ggccgtagag gccgaatcct tgcgtgcgtg gcagcaggcg cacattcggc
    29761 agctgcatca gccggctaag ggtggcatca cgccagatcg ccgcgtcctg accatcaatg
    29821 ccgcaccgcg cctccgccag cagcgatccg ccaagttcgg actgttcgtc gcacaggatc
    29881 acatcggcgc ccgcctcgcc cgcagccagc gctgctgcca ggccggaggg cccgcctccg
    29941 accaccagca cgtcgcaatg atcataatac tgaacataac ggtcgggatc cggatcgaca
    30001 ggccgcgcgc caaggccggc cgcatgacgc acgatcggct cgaacaggcg cgtccacagc
    30061 gtcgttccgg ccattttcgg tcccatgaag gtcttgtagt agaagccggc agagagaaaa
    30121 cgcgagaagc gatcgttgat ggcgccgatg tcaaggccca gcgacggcca gttgttctgg
    30181 ctgcgcgcct caaggccatc gtgaagcgga acttcggttg ccctgagatt gggcgtcatg
    30241 cgcccctttc cgcgatgcac cgagaccagc gcattgggct cctcgatgcc ggcggaaaga
    30301 atgccgcgcg gccggtgata cttgtaggag cggccgacaa aatgcacgcc attggcgagc
    30361 agcgcggagg ccagcgtatc gcccttgaag ccgcgatagg tcaggccgtc gaagctgaag
    30421 cgcagggggc ttgttcgatc aatccgcccc ccggcagcgg tgcgaaacga ttgtttcatg
    30481 ccatggtctc cggcagatcc ggacgtggct cactggctcg gtaggttgta atgaaacggt
    30541 cactcaccgt atcgcgcagg gcattgaaaa accgcccgca gccgtggata tggcgccagc
    30601 gttcagcatg caggcccttg gggttggtac gcacatagag aaactcagcc cactgatcat
    30661 cactgacgga atcggggttt tccgggcgaa cgagatgcgc ctcgccgcca tagcggaatt
    30721 cgatctccgg gcggtccata tcgcaatagg ggcagcggat cagcaacatg gttccctcaa
    30781 tgagccaccg cggccgcagc cgcctcgtca atcaggcggc cggtgcggaa ccggtcaagc
    30841 gagaacggag cggcgatcgg atgcggctcg ccgcgcgcca atgtgtgggc aaagacatgc
    30901 cctgatccgg gcgttgcctt gaacccgccg gttccccagc cgcaattgac gaacagcccc
    30961 ggaaccgggg tcagcccgat gatcggcgag cgatcgggcg tcacatcgac gatgccgccc
    31021 caggaacgca gcatgcgcag acgccgcacc gccggaaaca gctcgcagac cgcatcaagc
    31081 gtatgggtgg cgatatgcag ccctcccgtc tgcgaatagg atgtataggc gtcggttccc
    31141 gcgccaatca ccagttcgcc cttgtccgat tgcgacatgt aggcatgcat ggcgttggac
    31201 atgaccacgc agggcatcat cggcttgacc ggttcggaga cgagcgcctg caacggatag
    31261 ctttccagcg gcagacgaac gcccgccatc gccatgatca ccgatgtatg tcccgcagca
    31321 acgacgccga tcctgcgggt agcgatgaaa ccgcgactgg tctcaacgcc ggtcgccgcc
    31381 ccggcagcat cgcggcgaat gccaagcacc tggcaccgtt caagaatatc gacacccagg
    31441 cggtctgccg cccgggcata gccccaggcc accgcatcgt ggcgtgccgt gccgccgcgc
    31501 cgctgcaaag tggcaccgag caccgggtat ctcaaatcgc cgctgatgtt cagcagcggg
    31561 cagaagtcct tggcttgttc gggtgtgagc cattcattgt cgatgccggc gagccgattg
    31621 gcatgaacgt ggcgcttgag aacctgcaca tcatgatggg tctgggccag catcatgacg
    31681 ccgcgcggcg aatacatgac gttgtagttc agttcgtggc tgagcccttg ccacagcttc
    31741 atcgcatgtt cgtagagcgc ctcggactcc tcccacagat agttggagcg aacgaccgtg
    31801 gtgttgcgcc cggtattgcc gccaccgagc cagccctttt ccactacggc gatgttggca
    31861 aggccatgct ccctggccag ataataggcc gtcgccagcc catggccgcc tgcaccgaca
    31921 ataaccgcgt cataggcgga tttcggctcc ggcgagcgcc attgcgccgg ccagccagca
    31981 ttcttcttca atccctgcct caggaccgag agcgcggaga atcgcatccc tgataccacc
    32041 ccgcccctgt caaaccacgg tcgcgcagcg gctgcaagat cgcgcggcaa tcagaagcgg
    32101 gctaaagcca ggttcagcct caggcgtctg gccgcaaggg gaggcagtag cagacgccag
    32161 ccgccctgct gcggcgtggt ttccggcatc agaggccgtg tagggccggt gccggcgtca
    32221 aagccaagca tcgtgccggg ggcgaaggca cggccgctgg caagtccgat atccagcact
    32281 accgggcgct gtgaccggtt gatgagaatg agtgccagac cagcggcatc gctcgccgca
    32341 taggccgata cctcggcggc cccaagggtt gacaactcca gcgcccggtc gccgaagcgc
    32401 cgaccggccg gatcggcttg gcggtagagc ctgtaggcct cgcccagcca acccggcagg
    32461 cttccccagt gggttgccat cgccaccccg ccggtggcaa agcggccgag tgcgtcggcc
    32521 agtgccagtc ccgaagtcaa ttgcccggcg cctccgtaat tgaattcggt gatcgccagc
    32581 gccgttcccg gaaaccagcg atcaatcagc cgatgcagcg acggcagcaa tggcagagac
    32641 agagcgcccc cgctctgggc aagggcacgc ggaacccagc tctgctcgac aaaacccggt
    32701 tcggaaagcg aacgcggtgc atcgagccgg gcctgatcgt gggccggatc ctcgctgcgg
    32761 aacagaccgc ccttttccag aaaggcatac cagtgaatgt ccagggcatc gagcagacgg
    32821 cggccatcct gctccgaagc cctgcggaag gaatcgagat agaggccaag aaagctgcca
    32881 taggcgcggt ggcgcggcca gtcaggcgcg ttctggaagg tgacgaattc ggtagcgccc
    32941 cagcttgacg gcccgaacac ccgcgccgac gggtcgatcg tcttgatcgc acgcgcggcg
    33001 gcaatcgaac gatcgatgaa agccaggatc tgaagtcttc cgggaaacag ccgcgggtgc
    33061 gtctggtgcc agatgcccgg ttcattatcg agcgcatagc catggatgcc gccgggcgag
    33121 gcggaactgc catagcgttc ggccagatgg gcgaccaggc gcggcaggct gaccgtgccc
    33181 ggcacatcgg tcagcgacca gttggccggc acgaagcgcg gcgaaggggt tgcctccgct
    33241 tcaccaacgc gccccagccg gtcggcggcg agatagggcg agagcggcag cgtaacgagg
    33301 ctttttgctc cgatggccag agaggccgca tgcatcgtct cgatgacagc cgccggtcga
    33361 tcccagtccg aggcgggcac ttgcagtgct tccagcagaa acggaccatt ggaatggtga
    33421 tggttcttgc cggcatggca ggcattgttc agccagttgt agccggtcat cagatcgcca
    33481 cccaggcgcc gggccgtgac ccccgcggca gcgtcgagaa cggccgaggc aaggccgcca
    33541 tccatcacgc cgatctcgtt ggatccgtag atcagcgggc tgatcggccg gccggggccg
    33601 gtatcgtcaa tggtcaacga cgggatgagg ccggcttcga tccggccagg caggcctgtt
    33661 gcgacgacac cggccaggct gccgaaaagg acattgcgcc gatcaggctg cgccctgctc
    33721 aagacaccag gcccgacccg tacatggagg ggcggaaccg cgacgcccag aataccttgc
    33781 ccagcccgcc ccaggcggtc gcggcagccc gttcggcacg cgccctggca aacggccaga
    33841 gcgcaaccgc cagcaggccg gcgccaaccg cctgaatgcc gccaacggcc atccacttgg
    33901 cgataccgcc ccattcgacc ggctgaacca tgtgatggac gaacacgcgt atctggccgg
    33961 acaggaactt gcgcttgcgg acataagccc agttgagccg cttttcggcc gcccattcga
    34021 gaacctcggc ctcggccgcc caggcaaatc ggcgtccggt cagcacgagg cgcttgagca
    34081 ggagtgaatc ctcgccgccg gtctcgttga ggctggtttc gaaagttcct gcgccggcca
    34141 ggcaacgcgc ccggtcgaac atcgaattgt tggtgccgag ataggccgca agatcagtga
    34201 tgtcggcgcg atcggcacga tcgatccgcc gacagaaata gggggcaaag ccgccgatct
    34261 catgcactcc gtcggaccgg gccagaaccg ggccgaacac ggcgtcagca ccggtggaac
    34321 gcgcgacttc gaccaggcgg gccaaccagt ccggtcgggc ctgctcgtca tcgtcgagga
    34381 aggcaaccca gcgccccgtc gccgctgcca cgccggcgtt gcgggcggtg gcaacaccgg
    34441 gaaccggttc gctgaaatag cgcagcggtg caccatcctc tgccattgcc tcgaccagaa
    34501 agcgcgcgtt ggcatcaacg gaattgtcga caacgatgat gtccatcgcc gtctgttcgt
    34561 cgagccgctg ctgcatcaga ctggccagcg cgcgtttcag gctgggctga cggttgagcg
    34621 tgggaatgac gacggaaaca atgtccatga atcgtccagc ggataaggga atgttgacgt
    34681 ttggtcagcc agtctagggg cgagcggtaa ccaatctgct aagggccaga catgaaaggc
    34741 caatgccagc aggcgagcgg ccgggcgcca tgaccgacat cagcagtctc aaggccagtt
    34801 tcatcattcc ggggcaggcg ccgagcgcag ccggcaccgg ccgctggcgc gcgcggttgc
    34861 acggcctgct actgttcggc tttcttctgt tcacctttgt cggcacccgg cccttcgctg
    34921 aagccgacgc agcgacgcgc accgatggca atgtgctcga ccgcatcgcg gtgctcgggc
    34981 tttttggcct gtcgctgctg gtcttgtggc tgcatcgctg gcgcgtagcg cgcttcgcgc
    35041 taccagcgct gccggtattc gtgctcgtcg gctttgcgat ggccagcatt gtctggtccg
    35101 acttccccga tctgacgctg agacgcagcc tgctgttgtt cttcctcgcg gtcatcgcct
    35161 tcgcggtggc gatcggcacc gagaacctca ggcaattgca cacatggatg ttcgccacca
    35221 tgaccgcgat catcctgatc aaccttgccg gttgtgtgat cgtgccgtcc atcgccattt
    35281 ccgagatcgg cgtccgcggc atgtattcgc agaaaaacgt ggccggcatc gtggcactga
    35341 tcaccatgat ccttggcaca acctgggtat tgggatccgc aacacggcgc gagaccgtga
    35401 tcggcatgac cgcgttggtg ccgaccgcct tcttccttgt catcaccatc agcaagacct
    35461 ccatcaatct ggcggccctg ggcatcgtgg tgatcctgtt cttcgggatg atcgagcgcc
    35521 atggacgctc tttcatcttg tttgcgatcg cggtcgggtt gctgctggct gtcggtatca
    35581 cactatggct ggcgaccctc gagttcgaca tcaatgccgc catgaccacg ctcatcggcg
    35641 atacatcgtt caccggacgt gacgaactct gggcctttgc ccgcaatgcg gcggagaaac
    35701 gctactggct tggccacggc tatggcgcgt tctgggatgt tggaaccgcc aatgatccgc
    35761 tgatcagggc cgaggttggc agttggcttg cagccgtgca gatcggtgtc atcaatcagg
    35821 cccatcacgg ctatctcgaa ctctggctgc atctggggct accggcgacc gttcttgcaa
    35881 ccgtcattac gatatgggcg gccctttacg gcggactcca cgcctgtttc ggcttggcaa
    35941 agccggcgac acgggctgct ctcggcgcct ttgccgttct gctgctgctc tacctcgccc
    36001 acaatctcac ggaggccacg ctgttcatgc gcggcgcttc gttcaattca accgcattgc
    36061 tggcaacctt cctgattgcc cgtgcccgtg atttcgcgtt ccaggatctc gctcagccat
    36121 gacgacccga acacgcctcg ccattgtcct gtgcaccttc catcgcgagg cgctgctggc
    36181 caaagcgctc gacagcctgt cgcagcagcc cataccgcag ggacttgagg tcgagatcct
    36241 cgtcgtcgac aattccgatg ccggtacagc ccgggaaatc gtgaaccaga aggcagcggc
    36301 aagccctgtt ccgatgcgct ggctggcggc gcatccggcc aatatcgcag tggcccgcaa
    36361 tgccggggtt gcggcgacaa gcgccgattt catcgccttc ctcgacgatg accagcaggc
    36421 ggcgtcggac tggctccgtg cggttgccga aaacgtccga aatggcgcaa tcgacgcctg
    36481 gttcggtgcg atcacgccgc agttcgaggc cggcagcgcc ttgacgccga tgacccgaac
    36541 gttgttctcc cgctggactg atcggcccgc cggcgatcgc ttggtcgcgc tcggcccggg
    36601 caagaccgcc gaagtcagcc tttcaactgc caattccctg ttccgccgcg ctgcaatgct
    36661 gatcggaccg gggccatttg accccgcctt cggcaacggc ggcggcgagg attacgatct
    36721 gatttgccgc atgcagcagc gcggatcgaa aatgacctgg cagccggaga tgcaggcaag
    36781 cgagttcgtg cccgccagcc gctgcgatcc ttcctacctc agggggcgct tctatgccgg
    36841 cggacaggcc ttcgccgaag cggctgccaa agccagcgcc gcaccgggcc tgatgcgctg
    36901 gatcgtgcgc gccaaggcgc ttgtccaggc cgggctgctg tcgctgaaac tgccggccgc
    36961 agcatggcgc ggcggtgcaa gctggcttga ccacagctat gtctgggcgg gcgtgctggg
    37021 aaagctgtcg ttcggccaaa tcaagccgat ctatcgcgcc tccgacccga ggccagcaga
    37081 agcgaaccga tagcgattat accagtggcc ctcacaaatg acctgtcggt catttcccgg
    37141 gccgtccctc gca
//
LOCUS       DAOMXG010000004        36066 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_803_length_36066_cov_11.124073, whole genome shotgun sequence.
ACCESSION   DAOMXG010000004 DAOMXG010000000
VERSION     DAOMXG010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 36066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36066
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_803_length_36066_cov_11.124073"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2374
                     /locus_tag="PLQ11_00600"
     CDS             <1..2374
                     /locus_tag="PLQ11_00600"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRK23430.1"
                     /translation="EPADPLAAQPAPALDEEVARALDELKAEVETAKSESGRPAALEA
                     MAQSIESLRQEQETAKVPATASDTPASAPPTAPELPRGGNLVADMRAIAAGWFHNGRA
                     SDRINRGPGDVAMPEELTAAAADRGVSISHTEKSLKALTEKDFPCIALDKAGGSHVLL
                     GRPDKTTLLRKDGESAKPVRLSALQKIFSGVVFFVRPRPEPAGDTMAATSSRMAGPAA
                     ESSEVAAIKGIASTVAAEMFRTQKPLLLQLILATVLSNLFMFAMPLFSMAVYDRVVPH
                     LAWETLWALSLGVMIVLIADFAIRFIRLKLMDSVGLSTATALQTRLFSRLMHGRLKDL
                     PTAPGGVANGVREVEALCHVIPAVAISVAIDLPFFILATALIYSIAGFVAITPVLGAL
                     VMVAIHILPLIGEDAARKHSRYVGTQTNAVLEAMGAIESVKVTGAERVLLRRWERLVD
                     ASAYASHRNRLQGSLATQSSVVISQVVVVLAMIIGVYEISTGTMTIGAMTAAVLLIGR
                     MMAPISQMMSFIHRAHQLSSAAKTMLRIFSVPQEGAGDSSEAREETPINGHIAFRNVS
                     FTYPGEETPSLDSITLDIRPGEKVGLIGRVGSGKSTLLRLILRLHEPTSGTMMFDDRD
                     ARQISPRALRRVLGFMRQDSVLFDDTLQANICFGMETMRASDFERAVTVSGVKDFAAR
                     HPAGYGMRVGTRGERLSGGERQSVALARLLAENPKVFIMDEPTSAMDNALEARIVRDL
                     REIIAEKTFVVATHRAPLLSLVDRIIWMENGRIVADGPKADVMQRLSQAA"
     gene            2525..3403
                     /locus_tag="PLQ11_00605"
     CDS             2525..3403
                     /locus_tag="PLQ11_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23431.1"
                     /translation="MAGGGSGEEENYWPGFVDALTTMTMVLTFIMMILGVVIFSMSDK
                     ISKIMIKQIAEAAKIDISGGGSLDEVRDKIVAALKAQHREKSSAEDTDKITQFPLRGD
                     SYAAPQPASPNTGRTKLPSPVPNAINSDGKLAGNTASEQPLAGQHDEKKVAPPPSPED
                     RDPNKIEPDRRIEASTINEVAQKASGVTLSGDGAVLTVSFEKRVVQLDDAKTQEFRSV
                     VNNSNAVKTATKLEVRGYADVSVIGVTEARRIAYYRAMLIRKQLMMTGIDPERIAVRI
                     EDGASADGDLVKVFAK"
     gene            3489..5558
                     /locus_tag="PLQ11_00610"
     CDS             3489..5558
                     /locus_tag="PLQ11_00610"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRK23432.1"
                     /translation="MRSKSLASKIKVQSIRMAVVYTALVGLTAWQWHFVWEGVNHNIY
                     LNAMIFLVFLFGAALAAVDLRGMTNERLAFEALQEAYSDIQMGRIKGKEDPFWRHYRC
                     LEKGTVFTPPKLLGHLYELIVEELHRTKQMQISVATMQNLIAAIQHSNHQSRSLLQYM
                     SGLLVFLGLIGTFIGLMEMVSSVGGIIGGLANSEASGDAVKNLLGALQAPLTGMATGF
                     AASLFGLFTSLVLGLVNRFVSSAAHAIEAEFEAWLAGISQLENEKKSSGEGRGGEGGA
                     GGGSGSVEGVAPGDATFMAAPGVAAMITAMRSTNHTFEKTADAIMKLADKRKEENDTL
                     MATLAQMERLTAEQDSMRRAITALGSIRDDISGLRQLDYAQANRMVEGFSKIEHTLEE
                     SRIATSKGFEAMALQELEARKQARVETDLVLSKIDETKDEAIKQHLSTTDRLESLVQL
                     QASLSRLLQTTQDQTVDKIEDMQGSMSEIRAAHADLVQSLEHVRGDVTSGIKELLNRP
                     DTSPQIQQMSVSMDSTLVKGLGEVAQVLNTALAIMTSSMKDIAAKQADTAQALNVISD
                     TKELTTEIRSLGRSIDSGLSTGFAEMSRSFESIFLSYSELVQRMGTGGPAASAGAPAP
                     HMFQAPAAPSERVAVPVVDPVRQEQAREADAKKPTNEMTARQRIEHMEKLYESAKVRI
                     NDLLKSA"
     gene            5707..6213
                     /locus_tag="PLQ11_00615"
     CDS             5707..6213
                     /locus_tag="PLQ11_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRK23433.1"
                     /translation="MVKFAMPQAVSSVLRRLPFVKRDKGGKHMRAHSRHPCVLIAEMQ
                     IPDKGIKLDGMVIEVSRGGIRFREASTYILDRRGTPVVIHLSGHEFPGSIVNVDTSGY
                     GIKLDALIADDVVNSLVGGPNTGANAMRPNDSSGDPLSQPEAAAADEAGPAASNSEPT
                     AAEGPQPT"
     gene            6304..7089
                     /locus_tag="PLQ11_00620"
     CDS             6304..7089
                     /locus_tag="PLQ11_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRK23434.1"
                     /translation="MQLDSSIAAIVTGGASGLGEATARRLASHGVRVALFDLDEARGT
                     RVAGDIGGLFCKTDVTSEASVADALKAARSRHGIERIAVNCAGIAPGKRIVLKNRETG
                     ALAPQDLASFSKVVSVNLIGTFNVLAQSAAGMAGLEPLDEDGSRGVIVNTASVAAEDG
                     QVGQAAYAASKGAVKALTLPAARDLSQYGIRVVAILPGIFHTPMFDSIAEDFRKTLAA
                     AVPFPQRLGRPDEYAHLVEFICRNGMLNGETIRLDGAIRMAPR"
     gene            complement(7195..7271)
                     /locus_tag="PLQ11_00625"
     tRNA            complement(7195..7271)
                     /locus_tag="PLQ11_00625"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7235..7237),aa:Arg,seq:cct)
     gene            complement(7557..8252)
                     /locus_tag="PLQ11_00630"
     CDS             complement(7557..8252)
                     /locus_tag="PLQ11_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family protein"
                     /protein_id="HRK23435.1"
                     /translation="MKKILLCTASAIAVLGSASAFAADLGAPKKPVVVPVVDTFNPWM
                     VRVRALAVIPEDRSSVVAGGVAVAGRGNLDVSNSVVPELDITYFFNRNFAVELILGTT
                     PHTVKGAKTLAGVGDVGSSWLLPPTLTAQYHFHLTPTIKPYIGAGVNYTFFLDEKQKG
                     AFVAFNLKNTFGLALQAGVDIMLDKNWGINVDVKKLFLQPEYRAVLGPATVVTGKAKI
                     DPWLIGAGITYKF"
     gene            8515..9243
                     /locus_tag="PLQ11_00635"
     CDS             8515..9243
                     /locus_tag="PLQ11_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23436.1"
                     /translation="MSQSVACDWSQAARDRVVAIESNGDLLLAGGGLSRLAGLAWPSI
                     DRPAERRALKAALDAWLTGFEVDALAISGRDRWGRRAVRLRTVETAPERRIWVEAELL
                     GEGIAIAWPEPGPDPCWEEALAAEHPARRDRLGQWSALHGRSARTRLREPTGEPMVRR
                     IVFRGVVQSVRNGRQVVFVNFIGARDSQPAWMLTRRQVERWRAAGRDPATFVGKSLII
                     RGDISGRPPGRLTIGDPRQVQFED"
     gene            9516..11027
                     /locus_tag="PLQ11_00640"
     CDS             9516..11027
                     /locus_tag="PLQ11_00640"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HRK23437.1"
                     /translation="MPAWRAPSPIIARLRDRFARLCGAALAPALLAACASELLVVGPD
                     TPKPTVELPSGAPKVSGAETVAAQQHQRMLQAFGGEYRAAAAQEILDSLLIKLRNGSD
                     DPGQTYRVSILNSGLVNAFALPGGQLYVTRGLLLLANDQAEVASVLAHEIAHVTARHA
                     MERQELESRSVLVSRVRAEVLNNPGAAQLVRDQAQVAIASFSRQQELEADKIGVQTLA
                     TAGFDPYGAHRFLYSLGRNAEIQDRTAEARSKAEGLDFTTTHPTTPDRIQQALLAARQ
                     AGAPGQITVERNRWLQALTGMVYGEDSAEGFVRGRTFLHPRLGITLTAPEGFFLENSA
                     QAVLGMTPGAMQAMRLDSAKITPDKSLETFLHDNRIEGLTLTDVRPLMVNGLPAATGL
                     AQGSDWTFRIFAIRVGGQVYRLIMAARNFSPDVDQTFFASFETFRKLSAEEQERARPL
                     RIAVHIAKRGETAEDLSQRMGGVDRPLDRFYVLNGLENGAALEPGRAYKTIID"
     gene            complement(11109..11678)
                     /locus_tag="PLQ11_00645"
     CDS             complement(11109..11678)
                     /locus_tag="PLQ11_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019906096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HRK23438.1"
                     /translation="MMSSKKTTQREGFKTGEHIVYPAHGVGRIMAIEEQEIAGFKLEL
                     FVVTFAKEKMTLRVPTSKVVTVGMRKLSPAPTIEKALEVLTGRARIKRTMWSRRAQEY
                     EAKINSGDLIATSEVVRDLYRSEAQPEQSYSERQLYETALARLIDEIAAVESITETEA
                     MKRIDQSLAKNPKRSRGEAAESGDAEEAA"
     gene            complement(12040..12378)
                     /locus_tag="PLQ11_00650"
     CDS             complement(12040..12378)
                     /locus_tag="PLQ11_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HRK23439.1"
                     /translation="MTYVVLDNCIKCKYTDCVEVCPVDCFYEGENMLVIHPDECIDCG
                     VCEPECPADAIKPDTEPGLEKWLSLNAKYASIWPNITTKKEPLSTAKDFDGQSGKFEK
                     YFSEKPGSGD"
     gene            complement(12443..12757)
                     /locus_tag="PLQ11_00655"
     CDS             complement(12443..12757)
                     /locus_tag="PLQ11_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015824489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HRK23440.1"
                     /translation="MAGAEDLSRQRLDKWLWHVRLQPTRSKAALFIRAGYARLNGQRV
                     EDPAKSVRCGDVLTLALARETPVVRVEKILPRRLGAPLASAAYSRIDTGSGNRSAIEP
                     LP"
     gene            complement(12762..15920)
                     /locus_tag="PLQ11_00660"
     CDS             complement(12762..15920)
                     /locus_tag="PLQ11_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017483737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRK23441.1"
                     /translation="MPIPSLNDPFKLRGVTALLGPTNTGKTHFAIERMLGHASGMIGL
                     PLRLLAREVYERVVARVGREAVALITGEEKIRPREPRYWVATVEAMPRDLSVAFVAID
                     ECQLAADSDRGHVFTDAMLNRRGSEETILIGAATMRPLIEDLLPGANIIARPRLSKLS
                     FSGERKLTRLPPRSAIVAFSAEEVYAIAELLRRERGGAAVVLGALSPRTRNAQVALFQ
                     SGEVDLIVATDAIGMGLNLDIDHVAFAADRKFDGAGYRALTAAEMGQIAGRAGRHLRD
                     GSFGSTGRCPPLDEDIIAALEEHRFDPVRLIQWRNPDLDYASFARLQQSLAVVPTVPG
                     LVRARPADDERTFDILAARPSLAGYANSRAAVERLWDVCKLPDYRKLAPHVHADLIES
                     LFHPLVRDGRIRADWFKKELAACDRTDGDIATLSQRIAEVRTFTYCANRSDWLDHPAH
                     WQNVARALEDKLSDALHERLAQRFIDRRTSVLMRRLRENAMLEAEITELGDVLVENQH
                     LGRLDGFRFTPDARAEGPEAKALKAAAAKALAGEIEARARRLAEADDAAFVLALDGIL
                     RWIGSPVARLQAGDKLYEPRLVLLADEHLYGASRDAVEQRLARWLTTHIDKLLGPLRP
                     LEAGTGLNGAARGIGYQVAENLGVLERSKVADVVKGLDQTARGELRALGVRFGAHHLF
                     TAGLMKPAPRTLAAQLFALKHEPEATGVAEVAHLASSGRTSIPVDPAISKDLYRVAGF
                     RVCGGRAVRVDILERLADLIRPALAWRKDAGAPPPPGAYPGGGFVVTGSMTSLVGCAG
                     EDFASILTALGYRMEMKPVQAIPVAVEGVAPIVVPEAAGDTETVEATPENAAEAPAEG
                     AESGDAPADVAIDPTPDTAEEITAATDAAPAETAAAEPSLVAVWRPGRAEHDRPRHRP
                     RIPREGRADRQRRNDAPRVEQAPQSAQPEQADDGRRGRRPEQSGDSRSRDDQPRSNRS
                     RDDRPRDQRPVRTDRKEGDRPRDGDRRHGRDRGRPDRDKGRSGAEREQIWQDKPRVEK
                     QLDPDSPFAKLLALKAAMEGKS"
     gene            16617..16701
                     /locus_tag="PLQ11_00665"
     tRNA            16617..16701
                     /locus_tag="PLQ11_00665"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16651..16653,aa:Leu,seq:cag)
     gene            16861..18267
                     /locus_tag="PLQ11_00670"
     CDS             16861..18267
                     /locus_tag="PLQ11_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HRK23442.1"
                     /translation="MIVVVTFLLNAVLNFATGLAVAAVLGPTEFGRFAVASMAAIVLA
                     TTMFDWLRLSTVRHYNEESRIDRPQLRASLDRAYMIGAAVLILGTIVALAASLSGQTS
                     AISSGLILATAATAFANAQFDFWSALARSRFLNRTYALLVISKNVLALAAMIAVGLAY
                     HSAVATMAAFCGSVAVAIALVWARLRDAPSPPAQVDRALIATFLRYGMPVVAGNAIFQ
                     LIILINRWFVGTTLGYSDVGHLALATDICIRIFLSVGAGFDVLMFQKAVRLETTQGAD
                     AARSQIETNATLVLAILAPMAGGYYMVLPMFEALVVPTAYHGTFAAMSAILLPGVFAF
                     GVMQFAFGPAFQMAGRTYPFTVAALAGLTCDLVLLAAFVGRDALHFAYVQSAALCAAT
                     AVAGVYALRANRRLISVRDVAAVAVGTIAMMVAVIPMRSFATPALGLISAVLVGAAVY
                     GAVVVAFNIAGLRDKLNP"
     gene            complement(18447..19247)
                     /gene="phnE"
                     /locus_tag="PLQ11_00675"
     CDS             complement(18447..19247)
                     /gene="phnE"
                     /locus_tag="PLQ11_00675"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018690440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HRK23443.1"
                     /translation="MSGGIAREWQRFTLAERIARGVLYIVTAVAVVWSIKTIDIIPEF
                     LYDAPEQTADLFKRMWPFDWAFYPKAIHAALIETFHIATLGTILAILLALPLSFLAAR
                     NFTKSPVLNLVAQFIFVSSRSVNSLIWALLFVAVFGPGALAGVLSIAFRSIGFVGKLF
                     GEALEEAQRGPIEALIAAGAPKSMLLAKGYWPQVEPAFWSVTLLRWDINIRESAVLGL
                     VGAGGIGVALDSALNNLYWDQVGLILAVIFAVVIVTEVVTSIIRSRII"
     gene            complement(19244..20041)
                     /gene="phnE"
                     /locus_tag="PLQ11_00680"
     CDS             complement(19244..20041)
                     /gene="phnE"
                     /locus_tag="PLQ11_00680"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HRK23444.1"
                     /translation="MSLAASTVRPAAFPVNWPARLAWIGLALYTLYATSLLDLTWARF
                     ITGLDNGAKFLDRMFPPNVQADKLELISTGMLESIQIAVLATFFGVLLSMPLGLAAAR
                     NLAPGWLAWSARGLIAVFRSFHPVIVSILFVKAVGFGALAGILALIVSSMGFVSKLFA
                     EVVEEMNPKQVEAVRATGANFWNVLIFAVMPQVMARFIGLSVYQLDSNLRNSTMVGIV
                     GGGGIGAPLFTAYQRFDYDVVLTILIAIIALIMMTEIASGRIRRIFQ"
     gene            complement(20038..20799)
                     /gene="phnC"
                     /locus_tag="PLQ11_00685"
     CDS             complement(20038..20799)
                     /gene="phnC"
                     /locus_tag="PLQ11_00685"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010687325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HRK23445.1"
                     /translation="MSQALLSIRNLRKEYRAGQPVLKDISLDIDGNGVTAIIGPSGTG
                     KSTLIRCINRLVDPTSGEILFAGQDLAKLSGPALRMARRRIGMVFQEYNLVERLSVIE
                     NVLSGRLGYVPAWRAFLRKFPAEDVSRAFDLIDAVGLNQDFATRRADALSGGQRQRVG
                     IARAIMQEPALVLADEPTSSLDPKTSVEIMELMAEISQKRSVPVIVNIHNVDLARRYA
                     RRIIGMTGGEVVYDGPPDGLTHDHLKHIYGGEDFL"
     gene            complement(20796..21845)
                     /gene="phnD"
                     /locus_tag="PLQ11_00690"
     CDS             complement(20796..21845)
                     /gene="phnD"
                     /locus_tag="PLQ11_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007564854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_function: GO:0043225 - ATPase-coupled inorganic anion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HRK23446.1"
                     /translation="MVRALMMAAAVFAAAPALAQDSCPNRGQLDNAYCDANNDLVADT
                     PTDPARLKDPSTLIFAYSPVENPAVYQTVYQPFMDHLGSCTGKKIVYYPVQNNAAQIE
                     AMRSGRLHISGFATGVVGFAVNLAGAVPFAVIGSEKGVSGYKLFALVKSDSPYKSLPE
                     LKGRKVAHTSPSSNSGNLAPRVFFPDLGLKPDDDYKVLMSGGHDKSVMGVASGDYDMA
                     PVASDVYERMVARGAVKPDTLRLIWQSETFPTSSWSIPHDLKPELARKISQCFMDYKW
                     HEGLKKEYSGEDRFLPMTYKETWKPIRDVAEKSGTPYNRAAYDAQVKREAEAAAKKAA
                     EAAAKAAGQPQAPKP"
     gene            complement(21854..22930)
                     /gene="phnD"
                     /locus_tag="PLQ11_00695"
     CDS             complement(21854..22930)
                     /gene="phnD"
                     /locus_tag="PLQ11_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010687326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_function: GO:0043225 - ATPase-coupled inorganic anion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HRK23447.1"
                     /translation="MRGAIVAGLALTVSSWTLGITGAVAQDACANRGQLDILYCDADN
                     DLVADVPTDQKKWRDPSTLVWAYTPVEDPAVYANIFKPFTDHLEKCVGKKTVYYPVQS
                     NSAEIEAMRSGRLHFAGFSTGPTGFAVNLAGAVPFAAKGLGDEVRGYNLIAVVKASSP
                     YKTLADLKGKKVAHTAPSSNSGNLAPRVLFPEQGLKTDDDYKPLMSGGHDKSVLGVGS
                     GDYDMAAVASDVFDRMATRGTIKAEDFRIIYRSPVFPTSSFAHAHDLKPELAAKLREC
                     FYSFRFTPAMTKEFNGDDRFLPISYGKDWKVVREVAEKSGTPYNKAAYDAESKREADA
                     AAKKAAEAAAKAAQQPAAPAAPKP"
     gene            complement(23078..24064)
                     /locus_tag="PLQ11_00700"
     CDS             complement(23078..24064)
                     /locus_tag="PLQ11_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018261747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HRK23448.1"
                     /translation="MRQIRFAGAGGPEVIVLDAEAVVPKPGPGQVLVAVHAAGVNRPD
                     CLQRAGKYPPPPGATDIPGLEIAGRIVAVGEGVSASQIGREICALTISGGYADYAIAD
                     VTLCLPVPKGLSMIEAAGIPETYFTVYDNVVTRGRLTAGETILVHGGSSGIGSTAIQL
                     ACAFGATVHATAGTDEKCAFCVSLGATAAINYRTQDFVAEIKRLTGGQGVDVILDMVG
                     GAYIQRNISSLAVEGRLVQIAFLQTPVAEVNFMPVMLRRLTMTGSTLRPRSVALKAAI
                     AERLRAEVWPLLDAGTIRPVIDRVFPLEEARAAHALMESSAHSGKIILAMQS"
     gene            complement(24385..25785)
                     /locus_tag="PLQ11_00705"
     CDS             complement(24385..25785)
                     /locus_tag="PLQ11_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012649074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRK23449.1"
                     /translation="MTCDLLIVGAGPAGMAAAIAARRAGLSVVVADENPAAGGQVYRA
                     VMDGPLAKEDRLGADYRAGRPLAEAFAACGAQHLCRTTVFMIERQDAGGFTVSLAMPD
                     RACLIEAGHVMIASGALERPFPIPGWTLPGVMTAGAAQTLLKTSAIVPEGPTVLAGCG
                     PLLYLLAAQYAQAGIKLAAILDTTPRGNWLGALPHLPGFLASPYLFKGLKLLHEALSA
                     HRLIRNVSALEAKGENQLSSVEAVAGGRRITIPAATLLLHQGVVPQINLAMASGVSHL
                     WNKVRLAFEPSLSAAGETDIRGLFITGDSAGIAGAEAAAARGILGALAIADRIDPAGT
                     AARQGQRRDAVARLNRAQRGRAFLDALCRPASQFRIPADDVIVCRCEDVAAGDLRRLI
                     RQGAQGPNQLKVFSRAGMGPCQGRSCGLTLTELFAEQSGHSAQAIGHLRLRAPVKPVT
                     VGQMAGLEPYRSEDAG"
     gene            complement(25785..26087)
                     /locus_tag="PLQ11_00710"
     CDS             complement(25785..26087)
                     /locus_tag="PLQ11_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018236693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HRK23450.1"
                     /translation="MFRRLLEPVARVTITVDGQAVMAAEGDTVAAALLANGITRFRRS
                     PTSASPRGPFCAMGVCFECLVTIDGEPNRQACLIPVRDGMQVVTGAAAPDLLAERQ"
     gene            complement(26068..27204)
                     /locus_tag="PLQ11_00715"
     CDS             complement(26068..27204)
                     /locus_tag="PLQ11_00715"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRK23451.1"
                     /translation="MTDLSRCGNDCDIAVIGGGVVGLSVAYGLAMRGQAVTLLDEGDV
                     AYRASRGNFALVWVQGKGIDLPDYARWTLGSASAWKGFAETLQRDSGINVALEQRGGL
                     MACLSEEEQEERAAQLQRLHNAAPDSATVEMLDHQQMKALLPEIGSEVVGGTYCRLDG
                     HVNALRLFRALHQACIGRGIAYRPNATVESLMPAADGFRIVTSAGEFRARKVVLAAGL
                     GNATLAPMVGLSAPVRPQRGQVIVTEKLAPFLHYPLGTIRQTDEGGVMLGDSKEEVGF
                     DTGNSHQVLGQIAAHAVKVFPLLARAQIVRSWGALRVMSPDGYPVYDRSTGSPGAYLV
                     TCHSGVTLAAAHASVMAEAIASDIWPEAIAAFSARRFGDVPAAA"
     gene            complement(27207..28004)
                     /locus_tag="PLQ11_00720"
     CDS             complement(27207..28004)
                     /locus_tag="PLQ11_00720"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012649076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRK23452.1"
                     /translation="MKNGPLALIFHTLFATFLLAPMVVVCLVAFTPEGFLALPTNGFS
                     LRWFRRIAEYPEFSRAFQTSLVIATLSSMIALAFATPAAIAIARYRFAGRDALAALFM
                     SPLMIPHIVLGIAFLRFFSLVGLSGTTQGLVIAHVIVVFPFALRMVLASAAGMDKRIE
                     DAAVSLGASRSIVYRRVILPLMVPGLASGFALSFIQSFDEVTMTVFVASPTTTTLPVR
                     MFNYIQDSIDPLVCAISALLILGTVVLMLVLDRLYGLERLFVGDERN"
     gene            complement(28001..28861)
                     /locus_tag="PLQ11_00725"
     CDS             complement(28001..28861)
                     /locus_tag="PLQ11_00725"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012649077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRK23453.1"
                     /translation="MTPWDRHAFALAGPATLLFLGVVATPLAMTFLLSLNGWNSSQGI
                     VPVITAKNFVEIFSDSYFAEIFLRTLRISLWVTVICVLIGAPEAYILRRMREPWRSVF
                     ILVVIGPLLLSVVARTLGWALLLGGKGLVSTALQTLGLTSGPVSLMFTEAGVVIALVH
                     VLVPFMVLSVWAALSRMDPAVERAAQSLGASQATIFRRIVLPQAMPGILSGSIVVFSL
                     AASAFATPAIIGGRRLKVVSTLAYDEFLNTLNWPLGAALAVLLLVGIVAVVVGWNRVV
                     ERRYAQVFAS"
     gene            complement(28858..29898)
                     /locus_tag="PLQ11_00730"
     CDS             complement(28858..29898)
                     /locus_tag="PLQ11_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRK23454.1"
                     /translation="MSYLVLEGLQKAYGEVLAVERLDLAVAKGEFVSLLGPSGCGKTT
                     TLQMIAGFIEPTAGAIRLDGADLVRLPPEKRGLGIVFQSYALFPHMTVAQNVAFGLEM
                     RGIAKSEIGQRVTDVLALVGLSDKGERYPRQMSGGQQQRVALARALVIRPSILLLDEP
                     LSNLDAKMREEMQVELRQIQQRAGTTTILVTHDQHEALALSDRIVVMSAGKVEQIGAP
                     FDVYEHPQSAFVASFLGKTNVFAGEGLGQTVIVAGHTIPTGRSVAGPCTLAVRPEKLQ
                     LASADAAGLPGRVEARIFQGNHWLFQIATAGGPALVIRQNDGMGLPGIGDTVSLVIRP
                     QDALVTAREAAQ"
     gene            complement(29990..31018)
                     /locus_tag="PLQ11_00735"
     CDS             complement(29990..31018)
                     /locus_tag="PLQ11_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012649079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRK23455.1"
                     /translation="MSSRLKLFIGASLLAMAATAPALAQEALLVAGYGGSTEKIIREQ
                     IITPFEKAHNVKITYVAGNSTDTLAKLQAGKDKQEIDVALIDDGPMMRAVSLGFCAPL
                     QGVDQSKIHDVAKLPGGKAMGLGLIATGLMYNTKVFKENGWAPPTSWNDLKDPKYKGK
                     VVMPPLNNGYGLLTVVMLARMNGGGEKNIDPGFKVMKDAVGPSILAYEPSPAKHAELF
                     QTGQAIFGVWGSARVQSLAEAGVPVEFVYPAEGAPVVMTALCAVAKPTISPKAHAFVQ
                     HMLSAEAQSILADHAGFAPVNKDAKVQKAGMMPVGDLAKKLVAADWDTINPVRDEWNK
                     RWVREIER"
     gene            31133..32038
                     /locus_tag="PLQ11_00740"
     CDS             31133..32038
                     /locus_tag="PLQ11_00740"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRK23456.1"
                     /translation="MMSNLSRQLDAFRAVMLTGGMTAAAQTLFITQPAVSRLIKDLEH
                     DFGLRLFERHGNQIAATAEARRLFEEVERTYSGLSHLRAFADDLRGSRVGSLRIAALP
                     ALAIAFLPFCLARFRNQRPNLSIMLDGIPSHLVLERVASGLFDIGFAAVPAERPSLRL
                     TPVPGSLMVVMPAGHRLAGRTVLRAKDIAGESVIMLGRGSYLRHSIETALAQVHVETQ
                     IESPLAAIACAFVAQGLGITLVDPFTAADFLGRGVVARRFEPAIDVGYSLITSKDRPL
                     SQVANDFVASLGEATAAYLEEAQSR"
     gene            complement(32063..32689)
                     /locus_tag="PLQ11_00745"
     CDS             complement(32063..32689)
                     /locus_tag="PLQ11_00745"
                     /inference="COORDINATES: protein motif:HMM:NF018264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrsF family protein"
                     /protein_id="HRK23457.1"
                     /translation="MRTADLINALAADTTPAGNARSSLLRVVPLAVVVLAIVMGSAIG
                     LRPDATEAMPAIAMKLSITLSLATIALMLLLAMSVHLNLAGRLSVLAIVPVFALAFVV
                     MDLVTNSSAGAMTRLVGTKSSHCLVLIPALSSIPLAGFLAAARHGMPASPAGAGALAG
                     LAAAGIGASFYALNCMEDSPLFVAFWYSIATLIVTGAGAALGSRLLRW"
     gene            complement(32686..33234)
                     /locus_tag="PLQ11_00750"
     CDS             complement(32686..33234)
                     /locus_tag="PLQ11_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRK23458.1"
                     /translation="MAERGSDLWGDMMGRALAGDEATYRQLLMAMTPVLRAIVRRSAA
                     QHGGGETEDIVQDILLAVHLKRGNWDRSQPFRPWLFGVARHKIIDAYRRRGRTVAVPL
                     EDVEHELEAAPAPAADDAREAEHWLSRLGERDRAIVRAIAIEERPTLEVSRSLGMQEG
                     AVRVALHRALKKLAAFARGEAQ"
     gene            33416..33697
                     /locus_tag="PLQ11_00755"
     CDS             33416..33697
                     /locus_tag="PLQ11_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020180559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HRK23459.1"
                     /translation="MDRRMLSLALAGALTTALTAGATPAAAQGMEKCYGIALKGQNDC
                     KAGAGTTCAGTSKVDYQGDAWKNVPAGTCEKTASKSSKTGFGMLKEFKS"
     gene            33730..34620
                     /locus_tag="PLQ11_00760"
     CDS             33730..34620
                     /locus_tag="PLQ11_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HRK23460.1"
                     /translation="MLARPSAAPHSPRAGLPARAGIGLKSEHSEELRKGPSSIGFLEV
                     HAENYMGAGGQPHADLTALRALYPLSVHGVGLSIGSEQPLDATHLDRLRRVLERYQPE
                     SFSEHLAWSTHDGHYLADLLPVAYNEATLVRVVDHIDQVQTYLGRRMLLENPSTYVAF
                     SASDWDEVDFIAEVQRRSGCGLLLDVNNVAVTCANHGRDPIAYLDAFPVAAVGEIHLA
                     GYAEDCDSLGARLLIDAHGSPVATDVWQLYAHALTRTGPVATLIEWDNNVPALDRLIA
                     EATMAEGYLSAAAQRRDSAA"
     gene            34617..35375
                     /locus_tag="PLQ11_00765"
     CDS             34617..35375
                     /locus_tag="PLQ11_00765"
                     /inference="COORDINATES: protein motif:HMM:NF021364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HRK23461.1"
                     /translation="MSQATFVTALLDRDKPVPDGLRTWNGSDPLQRFRVYRNNVAVAL
                     IDTLAATFPVTLALCGEEFFRAMTRAFALGSPPASPLLADFGQDLPDFIAGYAAAQKV
                     PYLADVARLEAARALVWHAGDAAPLAAETFACWQDRIGEAVLTVHPAHAVLVSPYPVV
                     SIWAAHNGLGDLEDIDLSQPEEALVVRPNLETEIWRLPPGGAAFVRALEAGQSVADAA
                     MNTLADTPAFEPAMALSLVIRARLVTAIHSGACQ"
     gene            35372..35854
                     /locus_tag="PLQ11_00770"
     CDS             35372..35854
                     /locus_tag="PLQ11_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020180556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HRK23462.1"
                     /translation="MTRIASLVRPVIAALEALPYAVLALLARFSIAAVFWKSGQTKIE
                     GLAIDLIQGKVELGLPRVTASAIDLFREEYKLPLLPPELAAYLAAAAEHVFPVLILIG
                     LATRLSALALAIMTLVIQIFVYPDAYALHGTWLAVLLMLAKFGPGSLSVDAAIANRST
                     "
BASE COUNT         6802 a        11585 c        11147 g         6532 t
ORIGIN      
        1 cgagccggcc gaccctcttg ccgcacagcc ggccccggcc ctcgacgagg aagtcgcccg
       61 cgcgctcgat gaactcaaag ctgaagtcga gaccgccaag tccgaatcgg gacggcctgc
      121 cgccctcgaa gccatggctc agtcgatcga atcgttgcgg caggaacagg agactgccaa
      181 ggttccggcc accgcttccg acacgccggc ctctgcacca ccaaccgcgc ccgagttgcc
      241 ccggggcggc aatctggttg ccgacatgcg ggccatagcc gctggatggt tccacaacgg
      301 ccgggcgtcc gaccggatta accgcggacc cggcgatgtg gcgatgccgg aggaactgac
      361 cgctgccgca gccgatcgcg gcgtcagcat cagccacacc gagaaatcac tcaaggctct
      421 gaccgagaag gattttccct gcatagccct ggacaaggcc ggtggcagtc atgtgctgct
      481 tggcaggccc gacaagacca cgctgctgcg caaggacggc gagagcgcca aaccggtccg
      541 gctgagcgct ttgcagaaga tcttttccgg cgtcgtgttc ttcgtccgcc ccaggcctga
      601 acctgccggc gacacgatgg ccgcaacctc gtctcgcatg gctggcccgg ctgctgaatc
      661 gtccgaggtg gcagcgatca aggggattgc ctccacagtc gcggcggaaa tgttccggac
      721 ccagaagccg ctgctcttgc aactcattct ggcgactgtc ctgagcaacc tgttcatgtt
      781 cgccatgccg ctgttttcca tggcggtgta tgaccgggtg gtcccgcatc tggcctggga
      841 gacgctgtgg gccttgtccc tgggcgtgat gatcgttctc atcgccgatt tcgccatccg
      901 cttcatccgc ctcaagttga tggattcggt cggactttcg accgccaccg cgctgcagac
      961 acggttgttc agccgcctga tgcatggacg gctgaaagat ctgccaaccg cccctggtgg
     1021 ggttgccaat ggggtgcgcg aggtcgaggc cctgtgccat gtcatcccgg ccgtggcgat
     1081 ctcggtggcg atcgatctgc cgttcttcat cctggcaacc gctctgatct attccatcgc
     1141 cggctttgtt gccatcaccc cggttctggg agctctggtc atggtggcga tccacatcct
     1201 gccactgatc ggcgaagatg cggcgcgcaa gcattcccgg tatgtcggca cccagaccaa
     1261 cgcggttctg gaagcgatgg gcgccatcga atcggtcaag gtgaccggtg ccgagcgcgt
     1321 tctgctccgc cgctgggagc ggctggttga cgcctcggcc tatgccagcc atcgcaaccg
     1381 cttgcagggc agccttgcga cccaatcatc cgtggtgatt tcccaggtgg ttgttgtcct
     1441 ggcgatgatc atcggcgtct atgaaatttc gaccggcaca atgacgattg gcgccatgac
     1501 cgccgccgtt ctgctgatcg ggcgcatgat ggcgccgatc agccagatga tgtcgttcat
     1561 ccaccgcgcc catcagttgt ccagcgctgc caagacgatg ctgcgcatct tctcggtgcc
     1621 gcaggagggg gccggtgatt cctccgaggc ccgcgaagag acgccgatca acggccatat
     1681 cgccttcagg aatgtcagtt tcacctatcc gggcgaggaa acaccttctc tggatagcat
     1741 cacgctcgac atccggcccg gcgagaaggt gggtctgatc ggccgcgtcg gcagtggcaa
     1801 gagcactctc ttgcgcctca tcctgcgcct gcatgagccg acctcgggca caatgatgtt
     1861 tgacgatcgc gatgcacgcc agatttcgcc gcgggcattg cggcgggtgc tcggcttcat
     1921 gcggcaggac agcgttctct tcgacgatac gttgcaggcc aacatctgct ttggcatgga
     1981 gacgatgcgg gcgtccgact ttgaacgggc ggtcaccgtc agcggcgtca aggattttgc
     2041 cgcgcgtcat ccggccggct atggcatgcg ggtcggaaca cggggcgagc gtctgtccgg
     2101 cggcgagcgc cagtccgtgg cgctggcccg cctgctggcc gagaatccga aggtattcat
     2161 catggacgag cccacatcgg cgatggacaa cgctctggag gcccgcatcg tccgagatct
     2221 gcgcgaaatc atcgctgaga agaccttcgt tgtggccact caccgggcgc cgctgctcag
     2281 ccttgtcgac cgcatcatct ggatggaaaa cgggcgcatc gtcgccgatg gccccaaggc
     2341 cgatgtcatg cagcgcctgt cgcaggcggc ctgacgccgg gtaagcgacc cggtcaggac
     2401 aatgctaccc gtttcgttgg ccgccgatta acgaaatgat gaggtgttgc acgctaagga
     2461 tgtgggacga gtgcccgcat gcggcgggca gggtcagaac ccaggctggg tcgaggtcgg
     2521 ttccatggca ggcggaggct ccggcgagga agagaactac tggccgggat tcgtggacgc
     2581 gctcaccacc atgaccatgg tgctgacctt catcatgatg atcctcggcg tcgtgatctt
     2641 ctcgatgtcg gacaagattt ccaagatcat gatcaagcag atcgccgagg ctgccaagat
     2701 cgacatctcc ggcggcggca gcctggacga ggtgcgcgac aagatcgtcg ccgcactcaa
     2761 ggctcagcac cgcgaaaaaa gctctgctga ggacactgac aagatcaccc agtttcccct
     2821 gcgcggtgat tcctatgcgg ccccccagcc ggcgtcgccg aacaccgggc gcaccaaact
     2881 gccatcaccg gtgcccaatg ccatcaacag cgacggcaag ctggcgggca ataccgcttc
     2941 cgagcagccg cttgccggtc agcatgacga gaagaaggtg gccccgcctc cctcgcccga
     3001 ggatcgcgac cccaacaaga tcgagccgga ccgccgcatc gaagcctcga cgatcaacga
     3061 agtggcccag aaagcctctg gcgtcacgct gtcgggtgac ggcgccgtgc tgacggtgtc
     3121 gttcgaaaag cgggtcgtcc agcttgacga cgccaagacg caggaattcc gcagcgtggt
     3181 caacaattcg aatgcggtca aaactgcgac caagctcgaa gtgcgcggct atgcggatgt
     3241 ctcggtcatc ggcgtgaccg aggcccgccg tatcgcctat taccgggcca tgctgatccg
     3301 caagcagctg atgatgaccg ggatcgatcc cgagcgcatc gcggtccgca ttgaggatgg
     3361 cgcctccgct gacggcgacc tggtcaaggt ttttgccaaa tagccggtag aattaaccgt
     3421 ttgttgcctt tgcactgtta accttacccc catcaccggt ctgcgcaggg ccggcacggg
     3481 gtacgggtat gcgttcaaag agtcttgcgt ccaagatcaa ggtccagtcc atccgcatgg
     3541 ctgtggttta caccgcgctg gtcgggctga ccgcgtggca gtggcacttc gtctgggaag
     3601 gtgtcaacca caacatctac cttaacgcga tgattttcct cgtgttcctc ttcggcgcgg
     3661 cgctcgcggc ggttgacctg aggggcatga ccaacgagcg cctggcgttc gaggccttgc
     3721 aggaagccta ttccgatatc cagatgggcc ggatcaaggg caaggaagac cccttctggc
     3781 ggcattaccg ctgtctggag aagggaacgg tcttcacccc gcccaagctg ctgggccatc
     3841 tttatgaact gatcgtcgag gaattgcatc gcaccaagca gatgcagatt tccgtcgcga
     3901 cgatgcagaa tcttatcgcc gccattcagc actccaacca tcagagccgc tcgctgctgc
     3961 aatacatgag cggacttctg gtgtttcttg gtcttatcgg aaccttcatc ggcctgatgg
     4021 aaatggtcag ttcggtcggt ggtatcatcg gcggcctggc caattccgag gccagcggcg
     4081 atgccgtcaa gaacctgctc ggcgccttgc aggctccgct gaccggcatg gcgaccggct
     4141 ttgccgcctc gctgttcgga ctgttcacgt cactggttct gggtctggtc aaccggttcg
     4201 tttcctcggc ggcccatgcg atcgaggccg agttcgaggc ttggctcgcc ggcatctccc
     4261 agcttgaaaa cgagaagaag tcgagcggcg aaggccgtgg tggcgaaggc ggcgctggcg
     4321 gcgggtccgg ttcggtggag ggcgtggcgc cgggcgatgc caccttcatg gccgcaccgg
     4381 gcgtggcagc catgatcacc gccatgcgct cgaccaacca cacattcgaa aagaccgccg
     4441 atgcgatcat gaagctggcg gacaagcgca aggaagagaa cgacaccctg atggcgacgc
     4501 tcgcccagat ggagcgactg acagccgagc aggacagcat gcgccgcgcc atcactgcgc
     4561 tgggctccat ccgcgacgat atttccggcc tgcgtcagct cgattatgcc caggccaatc
     4621 gcatggtcga aggcttttcc aagatcgaac atacccttga ggaatcgcgt atcgccacct
     4681 ccaagggttt tgaagcgatg gccttgcagg agctcgaggc ccgcaaacag gcgcgggtcg
     4741 agaccgatct ggtgctcagc aagatcgacg agaccaagga tgaagccatc aagcagcatc
     4801 tgagcaccac tgaccgcctg gaatcgcttg tccagttgca ggccagcctc agccgcctgt
     4861 tgcagacgac acaggatcag acggtcgaca agatcgagga catgcaggga tccatgtcgg
     4921 agatccgcgc ggcccatgcc gatcttgtcc agagtctcga gcatgtgcgt ggtgacgtga
     4981 cctccggaat caaggaactg ctcaaccggc cagacacttc gccgcagatc cagcagatgt
     5041 cggtttcgat ggattcgacg ctggtcaagg gcctgggcga ggttgcccag gtgctcaaca
     5101 ccgcccttgc catcatgacc tccagcatga aggatatcgc cgccaagcag gccgataccg
     5161 cacaggctct caacgtcatt tccgacacca aggagcttac gaccgagatc cgttcgctcg
     5221 gccggtccat cgacagcggc ctgtcgactg gctttgctga aatgtcgcgt tcgttcgaat
     5281 cgatcttcct gtcctattcc gaactggtgc aacgcatggg aaccggtggg ccagcagcct
     5341 cggccggtgc ccctgccccg cacatgttcc aggccccggc cgcccccagc gaacgtgtcg
     5401 ctgttccggt ggtcgatccc gtgcgccagg aacaggctcg cgaggcggat gccaagaagc
     5461 ctaccaatga aatgaccgcc cgccagcgca tcgagcatat ggaaaagctc tatgaatcgg
     5521 ccaaggtccg catcaacgac ctcttgaaga gcgcctgacc agggcgcgtg gaacgactgg
     5581 agcatgcagc gatcaagcgg aagcaggtta accgccgcat gctccaaact tacagataac
     5641 gatcacgtta tctgtgtttt ggtgagtcca tcaaagcaca gcgtgatcta gagaagggcc
     5701 agatcgatgg tcaagttcgc catgccgcaa gcggtatcct cggttctgcg tcgcctgccg
     5761 ttcgtcaaac gcgacaaggg cggcaagcat atgcgggctc actcgcgtca cccctgtgtg
     5821 ctgatcgccg aaatgcagat cccggacaag ggcatcaaac ttgatggcat ggtcatcgag
     5881 gtctcgcggg gcggcatccg tttccgggaa gcgtcgacct acattctcga ccgccgcggc
     5941 acccccgtcg tcatccattt gtctggccat gaattccccg gatcgatcgt caacgtcgac
     6001 accagcggct atggcatcaa gctcgatgcg ctcatcgccg acgatgtggt caacagcctc
     6061 gtcggcggcc cgaacactgg cgccaacgcg atgcggccca acgattcttc gggcgatccg
     6121 ctctcccaac cggaggcggc cgcagcggat gaagccgggc cggcggcgtc aaactccgaa
     6181 ccgaccgctg cggaaggccc gcagccaacc tgaggcacgt ctcgaaggcc caggactttc
     6241 cccgttgcgc atgggcgcac gaaccggcat tgtcggccca tcaatcgaca acggatccgt
     6301 gccatgcagc ttgattcttc cattgccgcc attgtcacag gcggcgcctc cggccttggc
     6361 gaggcaaccg cccggcgcct tgcctcccat ggcgtgcgtg tggctctctt cgatcttgat
     6421 gaagcacgcg gcacccgtgt ggccggcgac attggtggcc tgttctgcaa gaccgatgtg
     6481 accagcgaag cctcggtggc cgacgcgctg aaggcggcac gcagccgcca tggcatcgaa
     6541 cgcattgcgg taaactgcgc cggcatcgcg ccgggaaagc gtatcgtcct caagaatcgc
     6601 gagaccggcg ccctggcgcc gcaggatctt gcctcattct cgaaggttgt atcggtcaac
     6661 ctgatcggca cgttcaacgt tctggcccag tcggcggcgg gaatggccgg gcttgaaccg
     6721 ctcgacgagg atggcagccg tggcgtgatc gtcaatacgg cctcggtcgc tgccgaagat
     6781 ggccaggtcg ggcaggccgc ctatgccgct tccaaaggcg cggtcaaggc actgacgctg
     6841 cccgctgcgc gggacctgtc gcaatacggc attcgcgtcg tcgccatcct gccgggaatc
     6901 ttccatacgc cgatgttcga ttccatcgcc gaagacttcc gcaagacgct ggcggcggcc
     6961 gtgcccttcc cgcaacggct ggggcgaccg gatgaatatg cccatctcgt cgagttcatt
     7021 tgccgcaacg gcatgctgaa cggcgagacc atccggcttg acggcgccat ccgcatggcg
     7081 ccgcgctgac cgggatacgc agcaatccgc cagcgacatg cgaccgaaat cgggcctcgc
     7141 agaagcaggc ccggccaaaa ctcaacggtt gcaggcgcgg gatcgatgca gttgtggcct
     7201 cccggaaggg attcgaaccc ctgacctacg gtttaggaaa ccgtcgctct atcctgctga
     7261 gctaccggga gacgtctaca ccgccagtcg tgtctatagg gcactcatct tccgaccgcc
     7321 agagccttct ctggccggtt cgggcgggat cagaggccat cgcccatcgc agcgcctcga
     7381 aggccctgcg ggcccggtat caagcctccc ggtgcggccg ctaggcagac cggtacgccc
     7441 cgccgcgccg ggcgaatgcc gcatgtcgaa cagcgagacc gatcccgttc gtcgcccaga
     7501 aagtcaaaag ccgccgtttc cggcggcttt cgcttgatca tccaaagttc cggaaatcag
     7561 aacttgtagg tgatgcccgc gccgatcagc cacggatcga tcttggcctt gccggtcacg
     7621 acagtcgccg gacccagcac cgcgcgatac tcgggctgca ggaacagctt cttgacgtcg
     7681 acgttgatgc cccagttctt gtcgagcatg atgtcgacac ccgcctgcag ggcgagaccg
     7741 aacgtgttct tcaggttgaa cgccacgaac gcgcccttct gcttctcgtc gaggaagaag
     7801 gtgtagttca cgccggcgcc gatgtagggt ttgatcgtcg gcgtcaggtg gaagtgatac
     7861 tgcgccgtca gggtcggcgg cagcagccag gagctgccga catcacccac gccggccagc
     7921 gtcttggcgc ccttgaccgt gtgcggcgtc gtgccgagga tcaattccac cgcgaagttg
     7981 cggttgaaga aataggtgat gtcgagttcc ggaacgaccg agttcgacac gtcgaggttg
     8041 ccacgcccgg caaccgccac gccgccagcc acaacactgc tgcgatcctc cgggatcact
     8101 gccagggcgc gaacgcgcac catccagggg ttgaaggtat cgacaaccgg cacgacaacc
     8161 ggcttcttcg gcgcgccgag atcggcggcg aaggccgaag ccgagccaag aacagcgatc
     8221 gcgctggcgg tgcaaaggag aattttcttc atgacagccc caaattcctg cgggttagac
     8281 cttcaagatc gcctcggcat taggacctcg cagggccttg ggcttcggct gatttaagga
     8341 tacgacgggt tgtgtgatcc cgtcccatat ctgctttttg agatggctcc cgacttgatc
     8401 tatgtcaaca gcactggggc gccgcacagc gtattcgccc gactgttgcg tttgcgccat
     8461 agtctttcgg cgatccatgt cgttttgaca tgcctaattg gcagtatctg tggattgtca
     8521 caatccgttg cctgtgactg gagtcaggcc gcgcgcgatc gcgtggtcgc catcgaatca
     8581 aacggcgacc tgctgctggc cggcggcggt ctgtcacgcc ttgccgggct ggcctggcca
     8641 tccattgacc gcccggccga gcgcagggcg ctcaaggctg ccctcgatgc ctggctgaca
     8701 ggcttcgagg ttgatgccct ggcaatttcg ggcagggacc gttgggggcg ccgggcggtg
     8761 cggttgcgaa cggtggagac cgcacccgag cgccgcatct gggtggaggc cgaacttttg
     8821 ggcgagggca tcgccatcgc ctggcctgaa ccggggccgg atccctgttg ggaggaggcg
     8881 cttgcggccg aacatccggc acgccgggat cgcctcggcc aatggtcggc gctgcatgga
     8941 cgcagtgccc ggaccagatt gcgcgaaccg accggcgagc ctatggttcg ccgcattgtg
     9001 ttcaggggtg tggtgcaatc ggtgcgcaat ggccgccagg tggtattcgt gaatttcatc
     9061 ggcgcgcgag acagccagcc agcctggatg ctgacacgtc gccaggtcga gcgatggagg
     9121 gccgcagggc gtgatcctgc cacatttgtt ggcaagagcc tgatcattcg cggtgacatc
     9181 agcggtcgcc cgccggggcg gctgacgatc ggcgatccgc ggcaggtgca gtttgaggac
     9241 tagagcatgt ttccttcata cggaaccatt ctgcggtgca gggtttgggc gaagggccgg
     9301 gaaattcccg aagagcatac cccgtgtatg gtcgagggga ttttccgagt attttgccca
     9361 aaacctgccc gtcctccgga tttccgggcg gcgggcgccc agtttggaag ccggcttgac
     9421 cgtatcgaca gatacgctct gcgccgcctt ccgcctggat cgcctccccg cccgagaaac
     9481 agaattgctt ccgtatgaag gaaacaggct ctagaatgcc agcttggcgc gcgccctccc
     9541 cgatcatcgc tcgcctgagg gaccggttcg cccggttgtg cggggccgcg ctggcgcccg
     9601 cgctgctggc ggcctgcgcc agcgaacttc tggtggttgg gccggatacg cccaagccga
     9661 ccgttgagtt accctccggc gctccgaagg tcagcggcgc cgagacggta gcggcacagc
     9721 agcaccagcg catgctccag gctttcggcg gcgaataccg cgccgccgcc gcccaggaaa
     9781 tcctcgattc gcttctgatc aagctgcgca atggcagcga tgaccccggc cagacctatc
     9841 gggtgagcat cctcaattcc ggattggtca atgccttcgc gctgccgggc ggccagctct
     9901 atgtcacgcg cggactgctg ctactggcca atgatcaggc cgaggttgcc tcggttctgg
     9961 cccatgagat tgcccatgtg acggcccgcc atgcgatgga gcggcaggag cttgaaagcc
    10021 gctcggtgct ggtcagccgt gtccgggccg aggtgctgaa caatcccggc gcagcccagt
    10081 tggtgcgcga tcaggcgcag gtcgccatcg ccagcttttc gcgccaacag gagcttgagg
    10141 ccgacaagat cggcgtgcag acgctggcga cggcgggatt tgatccttat ggcgcgcatc
    10201 gctttctcta ttcgctcggg cgcaacgccg aaattcagga ccggaccgcc gaggcccggt
    10261 caaaggccga ggggctcgat ttcaccacga cccatcccac cactccggat cgcatccagc
    10321 aggcgctgct tgctgcccgt caggccgggg cccccgggca gatcacggta gagcgcaacc
    10381 gttggctgca ggcactgacc gggatggtct atggcgagga ttcagccgaa gggttcgtgc
    10441 gcggccggac gttcctgcat ccgcggctgg gcattaccct caccgcgccg gaggggttct
    10501 ttctggaaaa ctcggcacag gccgttctgg gcatgactcc gggcgccatg caggccatgc
    10561 ggctcgacag cgccaagatc acgccggaca agagcctgga aacattcctt catgacaacc
    10621 gcatcgaagg actgaccctg accgatgtgc gcccgctgat ggtcaacgga ttgcccgccg
    10681 ccaccggact ggcgcaaggc agcgactgga ccttccggat cttcgccatt cgcgtcggcg
    10741 ggcaggtcta tcgcctgatc atggccgcgc gaaacttcag cccggacgtc gatcagacgt
    10801 tttttgccag ctttgaaacc ttccgcaagc tcagcgccga ggaacaggag cgggctcggc
    10861 cgctcaggat tgccgtccac atcgccaaac gcggcgagac cgccgaggat ctctctcagc
    10921 ggatgggcgg agtcgaccgg cctctggacc ggttctatgt cctcaacggg ctggaaaacg
    10981 gcgccgcgct ggagcctggc cgcgcctaca agaccatcat cgactgatgg ctggcaccgc
    11041 ccggatagca aaagacccgg agggaggcct ccgggtcctg atgcagatgc tctggtcagc
    11101 cagctggctc aggcagcttc ctcggcgtcg cccgattcgg cggcttcgcc gcggctgcgc
    11161 ttggggttct tggcaagact ctgatcgatg cgcttcatcg cttcggtctc ggtgatgctc
    11221 tccacggcgg cgatctcatc gatcaagcgc gccagtgccg tctcgtaaag ctgccgttcg
    11281 gaataggact gctcgggctg ggcttccgac cgataaagat cgcgcacgac ttcggacgtg
    11341 gcaatcagat caccggaatt gatcttggct tcatattcct gagcgcggcg gctccacatg
    11401 gtccgcttga tgcgcgcccg cccggtgagc acttccagcg ccttttcgat cgtcggagcc
    11461 ggcgacagct tgcgcatgcc gacggtgaca accttcgagg tcggcacacg cagggtcatt
    11521 ttctctttgg cgaaggtcac cacgaacagt tccagcttga aaccggcaat ctcctgttcc
    11581 tcgatcgcca tgatgcgacc gacgccatgg gccggataca cgatatgctc gccggtcttg
    11641 aaaccttcgc gctgcgtcgt cttcttggag ctcatcaaag tccctcgttg ccgaaccggc
    11701 cccttaacgg aaaccggccc gctcttttcc atccgcgccc cggcgcagat catgtcacca
    11761 agctgctccc ggcctgagcc ggaaaccgca gtccctcgtc gagtatgctt ggcagagcaa
    11821 gaccggagac tctacgcaca accacccatg gccagcccga tggagcatgg tgcacaggac
    11881 acgatcgcgc gggaagtcag ataaggttca agggctgccg ccagttcgac tgtatgcaag
    11941 ccctaccaca aaaatcggcc aaaatcaaag gttaggtagc caagcgttta ccacggttaa
    12001 ttttcttcac cgaacggtaa ccttaccagc ttggcctgat cagtccccgc tgccgggctt
    12061 ttccgaaaag tatttttcga actttccgga ctggccatcg aagtctttgg cggtagacaa
    12121 cggctctttc tttgtcgtga tgtttggcca gatcgacgca tatttggcgt tcagcgacaa
    12181 ccatttctcc agacctggct cagtatccgg cttgatcgca tcggccgggc actccggctc
    12241 gcagacgccg caatcgatgc attcatccgg atggatcacc agcatgttct cgccctcgta
    12301 gaaacaatcg acggggcaga cctcgacaca atcggtgtat ttgcacttga tgcagttatc
    12361 gagcacgaca taggtcacgg gatgcactcc gttatgcgcc acacgggcca aattcgcccg
    12421 ctctgctact agaccggggc tttcagggca agggctcaat tgcggatcgg ttgcccgacc
    12481 ccgtatcgat gcggctgtag gcggcgctag ccagcggtgc tccgagacgg cggggcagga
    12541 ttttctcgac gcggacaacc ggagtttcgc gcgccagcgc aagcgtgagc acatcgccgc
    12601 atcggacact ttttgccggg tcctcgacgc gctggccatt gaggcgcgcg tagcctgccc
    12661 ggatgaacag ggcggccttg ctgcgggtcg gctgcaggcg cacatgccaa agccatttgt
    12721 cgaggcgctg acgggagaga tcctccgccc cggccatgcg ctcaggactt gccttccatc
    12781 gccgccttca gcgccagaag cttggcgaaa ggcgaatccg gatcaagctg cttttccacc
    12841 cggggcttgt cctgccagat ctgctcgcgc tcggcgccgg agcgtccctt gtcccggtcg
    12901 ggccggcccc ggtcgcgacc gtgccggcga tcgccgtcac ggggcctgtc accctccttg
    12961 cggtcggtgc gcaccgggcg ctgatccctt gggcggtcat cgcgagagcg gttgctgcgc
    13021 ggttgatcat cgcgcgaccg gctgtcgccg gactgctccg gtcgcctgcc gcgtcgacca
    13081 tcgtcagcct gttcaggttg agccgattgt ggcgcctgtt cgacgcgcgg cgcatcattc
    13141 cggcgctggc ggtcggcccg gccctcgcgc ggaatgcgtg gccggtggcg ggggcgatca
    13201 tgctcggccc gtcccggacg ccagacagcg acgagggaag gctccgcagc ggctgtttcg
    13261 gccggcgcag catcggtcgc cgccgtgatc tcttcggccg tatccggagt gggatcgatc
    13321 gccacatccg ccggcgcatc cccggattcg gcaccctcgg ccggcgcttc ggcggcattc
    13381 tcaggggtcg cttccaccgt ttctgtgtcg cctgcggcct ccggtacgac gatcggcgcc
    13441 acgccctcga cggcgaccgg gatggcctgg accggcttca tctccatccg gtagccaagc
    13501 gcggtcagga tcgaggcaaa atcctcgccg gcacagccga caagcgaggt catgctgccg
    13561 gtgacaacga acccgccgcc gggataggcg ccggggggcg gtggcgcgcc ggcgtccttg
    13621 cgccaggcca gcgccggacg gatgagatcg gcaagccgct cgagaatgtc gacgcgcacc
    13681 gcccgtccgc cgcagacacg gaaaccggca acccgataca gatccttcga gatggccgga
    13741 tcaaccggaa tcgaggtgcg gccggagctt gccagatgcg ccacctcggc aacgccggtc
    13801 gcctccggct cgtgcttgag ggcgaaaagc tgagcggcaa gcgtgcgtgg cgccggcttc
    13861 atcaggccgg cggtgaacag atgatgggcg ccaaatcgga ccccgagggc gcgcaattca
    13921 ccacgcgcgg tctggtcaag acccttgacg acatcggcaa ccttcgaccg ttccagcacg
    13981 ccgaggtttt cggcgacctg atagccgatg ccgcgggccg caccgttgag cccggttccc
    14041 gcctccagcg gacgcaaggg gcccagcagc ttgtcgatat gggtggtgag ccaccgggca
    14101 agccgttgtt cgacggcatc gcgcgatgct ccgtaaagat gctcatccgc cagcagcacc
    14161 agccttggct catacagttt gtcgccggcc tgcagccgcg ctaccggcga accgatccag
    14221 cgcaaaatgc catccagcgc cagcacgaag gcagcatcat cggcctcggc cagccggcgg
    14281 gcgcgggcct cgatctctcc ggcgagagcc ttggcggcgg cggctttcag cgccttggct
    14341 tccggcccct cggcgcgggc gtcaggggtg aagcgaaatc catcgaggcg ccccagatgc
    14401 tggttctcga caaggacgtc gcccaactcg gtgatctcgg cttccagcat cgcattctct
    14461 ctcaggcgcc gcatcaggac ggatgtgcgg cgatcgatga agcgctgcgc gagtctttcg
    14521 tgcagagcat cggacagctt gtcctctagc gcgcgcgcaa cgttctgcca atgcgcagga
    14581 tgatcaagcc agtcactgcg gttggcgcaa taggtgaatg tgcgcacctc ggcgatgcgc
    14641 tgggacaggg tggcaatgtc gccgtcggtg cggtcgcagg cggcaagttc cttcttgaac
    14701 cagtccgcac ggatgcgccc gtcgcgcacc agcgggtgga acagggactc gatcaggtcg
    14761 gcatgcacat gcggggccag tttgcgatag tcgggcagct tgcagacatc ccacaggcgc
    14821 tcgaccgccg cgcggctgtt ggcatagccg gcaagcgacg ggcgggcagc caggatatcg
    14881 aaagtccgtt catcgtcggc ggggcgggcc cgcacaagtc ctgggaccgt cggcacaacg
    14941 gcaaggctct gctggaggcg ggcgaaggag gcataatcga ggtccggatt gcgccactgg
    15001 atcagccgca ccggatcgaa ccgatgctct tcgagcgcag caatgatatc ctcgtcgagc
    15061 ggcgggcagc ggcctgtgct gccaaaactg ccatcgcgca agtgccgtcc ggcgcgtccg
    15121 gcgatctggc ccatttccgc cgcggtcagc gcccgatagc ccgccccgtc gaacttgcgg
    15181 tcggcggcga aggcaacatg gtcgatatcg aggttcaatc ccattccgat ggcgtcggtc
    15241 gccacgatca gatcgacctc tccagactgg aacagggcaa cctgggcgtt gcgcgtgcgc
    15301 gggctgagcg cgccgagcac cacggccgcc cctccccgct cgcgtcgcag cagttcggcg
    15361 atcgcgtaaa cttcttcggc cgaaaatgcg acaatcgccg agcgcggcgg caggcgtgtc
    15421 agtttgcgtt caccggaaaa gctgagcttt gacagccgcg ggcgggcgat gatgttggcg
    15481 ccaggcaaca ggtcctcgat caacggccgc atggtggccg cgccgatcag gatggtttcc
    15541 tcactgccgc gacggttgag catggcatcg gtaaagacat ggccgcggtc ggaatcggcc
    15601 gccagctggc attcatcgat ggccacgaac gcgaccgaaa gatcccgcgg catcgcttcg
    15661 acggtcgcca cccagtatcg gggctctcgc gggcggatct tctcctcgcc ggtgatcagg
    15721 gccaccgctt cacgaccaac gcgcgcgaca acccgctcat agacctcacg cgccagcaac
    15781 ctgagcggca ggccgatcat tcccgacgca tgccccagca tacgctcgat ggcaaagtgt
    15841 gtcttgccgg tgttggtcgg ccccagcaac gctgtgacac cgcgcaattt gaacggatcg
    15901 ttgagactgg gaatcggcat ggttggctcg ctagatcacg ccgcattttg atggaatcat
    15961 caaaatgcgg ataacgtgat cggtatcaat aagttagagc ttgtcttctg cgattgaccg
    16021 gtatccactt aatcgcgaca tgctctagcc gtttcactgg ctcgaatgga tggcattctc
    16081 aaggaaaaag cgtgccggaa cggaacgctt tgggagaaat cggcgccggt cgcagaaagg
    16141 cttcgaacgg gaaaagaaca aaaagtcgac gaatcactga ctcgcttcga gtcttttccc
    16201 tttccgtcag agaaatgccg gtagttcacc cggaaatgcc gcagtgccgc ccaagaaaat
    16261 cgccgggtga gcgacgatca aattaacctt tggactcgcg cctgaacacg cccgctcttc
    16321 aggattcgtc cccgttgcgt ctctttatcg ttactgaacc atgaacggtg ctggcttgac
    16381 catgaagcag gccgtttacc tttggccaga acagcgaaca aagcatcgac gaatcactga
    16441 cctggggctc ggaaccccgc agctcttccc ggcagtgccc tggctcgaaa tcccgccggc
    16501 ccggaatatg tcgcgccacc gaccttgcca tgcacaccaa ggagtcgccc aggcgccagg
    16561 atgctgcgca cggcgattga caaggccgga tgcgggccct aatgagcacc ctcgttgccc
    16621 aggtggcgga attggtagac gcgctggttt caggtaccag tggtgcaagc cgtggaggtt
    16681 cgagtcctct cctgggcacc atccctttct gagggatggt ccgctgactt atctcttctg
    16741 acaacgcagt gttgggtgcc gaccgttcgt ctttgcgatg ccgggccccg ccgtaatttg
    16801 acgctttctt agaggaagct ggctaagcct tgttgtcatg aaacactcgc tccccgaacc
    16861 atgattgtag tcgtcacctt cctgctcaat gccgtgctga atttcgcgac agggctggcg
    16921 gtggcggctg tcctggggcc gaccgagttt ggccgcttcg ctgttgcctc gatggctgcc
    16981 atcgttctgg caacgacaat gttcgactgg ttgcggctgt cgacggtccg gcactacaac
    17041 gaggaaagcc gcatcgaccg gccgcaactc agggccagcc tcgaccgggc ctacatgatc
    17101 ggtgcagccg tcctgatcct ggggaccatt gtcgctctgg ccgccagcct ttccggccag
    17161 accagtgcca tatcaagcgg cctcattctg gccacggcag ccacggcctt tgccaatgcc
    17221 cagttcgatt tctggtcggc cctggcgcgg tcccgctttc tcaacaggac ctacgccctg
    17281 ctggtgattt ccaagaatgt gctggctctg gcggcgatga tcgccgttgg actggcgtat
    17341 cactcggcgg tggccaccat ggccgccttt tgcggcagcg ttgcggttgc catcgcgctg
    17401 gtctgggcca ggctccgtga tgcgccaagc ccgccggcac aggtcgaccg cgcgctgatt
    17461 gcaacatttc tgcgctatgg catgcccgtc gtggccggca atgcgatatt ccagctcatc
    17521 atcctgatca accgctggtt tgtcggcaca acgcttggct attcggatgt cggccatctt
    17581 gcgctggcga ccgatatctg tatccgcatt ttcctgtcgg tcggcgcggg cttcgacgtg
    17641 ctgatgttcc agaaggccgt ccggcttgaa acgacgcagg gggccgatgc cgcccgctcg
    17701 cagatcgaga ccaatgcgac ccttgttctt gcgatcctgg ctccgatggc cggcggttat
    17761 tacatggtgc tgccgatgtt cgaggcgctg gtggtgccga cagcctacca cggcacgttt
    17821 gcagccatgt cggccatcct gctgcctggg gtttttgcct tcggcgtcat gcaatttgcc
    17881 ttcggccccg cctttcagat ggcgggccgc acctatcctt tcaccgtcgc ggcgctggca
    17941 gggctgacct gtgacctcgt cctgctagca gcctttgtcg ggcgggatgc cctgcatttt
    18001 gcctatgtgc agtcggcagc cctgtgcgcc gcgacggcag tggccggggt ctatgcacta
    18061 cgtgcgaacc gccggctgat ctcggtacgg gatgtggcag cggttgcagt gggtacgatc
    18121 gccatgatgg tcgctgtcat accgatgcgc agcttcgcga caccggcctt gggcctcata
    18181 tccgcggtcc tcgtcggcgc agcggtctat ggcgccgtcg tcgttgcctt caacatcgcc
    18241 ggcctgcggg acaagctgaa cccttgatca gacgcatcgg cgcaccaggc aatgcacctt
    18301 ccgcgagcag ccgagcctag agcatgccgc attttgatgg aatcatcaaa atgcggatca
    18361 cgtgatcggt atcaataagt tagagcatgt cttctgcgat taatcggtat ccacttaatc
    18421 gcgacatgct ttagagcatt ctccgttcag atgatccgcg accggatgat cgaggtgacg
    18481 acttcggtaa cgatcacgac agcgaagatc acggccagga tcagcccgac ctgatcccag
    18541 tagagattgt tgagggctga atcgagcgca accccgatcc cgcccgctcc gaccagcccg
    18601 agcacggcgc tctcgcggat gttgatatcc cacctgagca gcgtcaccga ccagaaggcc
    18661 ggctccacct gcggccagta gcctttggcc agcagcatcg actttggcgc tccggccgcg
    18721 atcagcgcct cgatcgggcc gcgctgggct tcctccagtg cctcgccgaa caacttgccg
    18781 acaaacccga tcgaccggaa cgcaatggaa aggaccccgg ccaaggcgcc tggcccgaaa
    18841 accgccacga acaacaaggc ccagatcagc gaattgaccg aacgcgacga cacaaagatg
    18901 aactgagcga ccagattcag caccgggctt tttgtgaaat tgcgcgccgc cagaaacgac
    18961 aatggcaagg ccagcaggat ggcgaggatc gtgcccagcg tggcgatatg gaaggtttcg
    19021 atcagtgcgg catggatggc cttgggatag aaggcccagt cgaacggcca catgcgcttg
    19081 aacagatcgg cggtctgctc cggcgcatcg tagaggaatt cgggaatgat atcgatggtc
    19141 ttgatcgacc agacgaccgc gaccgccgtg acgatgtaaa gcaccccgcg ggcgatccgc
    19201 tcggccagcg tgaagcgctg ccattcgcgc gcgatgccgc cgctcactgg aagatcctcc
    19261 ggatgcgccc ggaggcaatc tcggtcatca tgatcagtgc aatgatcgcg atcaggatgg
    19321 tgagcacgac atcgtaatca aagcgctggt aggcggtgaa cagcggcgca ccgatgccgc
    19381 cgccgccgac aatgccgacc atcgtcgagt tcctcaggtt ggaatcgagc tggtacacgg
    19441 aaagcccgat gaagcgggcc atcacctggg gcatcaccgc gaagatcagc acgttccaga
    19501 aattcgctcc cgtggcgcgc accgcctcga cctgcttggg gttcatctct tccaccacct
    19561 cggcgaacag cttggacaca aagcccatcg acgagacgat cagcgccaga atgccggcca
    19621 gcgcgccaaa accgacggcc ttgacgaaca ggatcgagac aatcaccgga tgaaacgagc
    19681 gaaagacggc gatgaggccg cgcgcgctcc atgccagcca gccaggcgca agattgcgcg
    19741 ccgcagccag cccgagcggc atcgacagca gcacgccgaa gaacgtcgcc agcacagcaa
    19801 tctggatcga ttccagcatg ccggtcgaga tcagctccag cttgtccgcc tgcacattgg
    19861 gcggaaacat gcgatcgagg aacttggcgc cattgtccag accggtaatg aaccgcgccc
    19921 aggtcagatc aagcagcgag gtggcataga gcgtgtagag cgccagcccg atccacgcca
    19981 gacgcgccgg ccaattgacc ggaaaggcgg cagggcgaac ggtggaggcg gccaggctca
    20041 caggaaatcc tcgccgccgt agatgtgctt gaggtggtcg tgcgtcagcc cgtccggcgg
    20101 accgtcatag acaacctcgc caccggtcat gccgatgatg cgccgggcat agcgccgggc
    20161 aaggtcaaca ttgtgaatgt tgacgattac cggtaccgag cgcttctggc tgatctcggc
    20221 catcagttcc atgatctcga ccgaagtctt gggatcgagc gacgaggtcg gctcatccgc
    20281 aagcaccagc gccggctcct gcatgatcgc ccgggcgatg ccgacgcgct gacgctggcc
    20341 acccgacagc gcatcggccc tgcgggtggc aaaatcctga ttgagcccga ccgcatcaat
    20401 caggtcgaag gcgcggctga cgtcctcggc cgggaacttg cgcaggaagg cccgccaggc
    20461 cggcacatat ccgagccggc ccgacagcac gttctcgatg acgctcagcc gttcgacgag
    20521 gttgtattcc tggaacacca tgccgatgcg gcgacgcgcc atgcgcaggg ccggaccgga
    20581 gagcttggcc agatcctgcc ccgcgaacag gatttcgcct gatgtcgggt cgacgagccg
    20641 gttgatgcag cggatcagcg tcgattttcc ggttcccgaa gggccgatga tcgccgtgac
    20701 accgttaccg tcaatgtcga gcgatatgtc cttgagcaca ggctggccgg cccgatactc
    20761 cttgcggaga ttgcggatgg acagcagcgc ctggctcatg gcttgggcgc ctgcggctgg
    20821 ccggcggcct tggcagccgc ctcggccgcc ttcttcgctg cagcctccgc ttcgcgcttg
    20881 acctgggcat cataggcggc ccggttgtaa ggcgtgccgg acttttccgc cacgtcacgg
    20941 atgggcttcc aggtctcctt gtaggtcatg ggaaggaaac ggtcctcgcc ggaatattcc
    21001 ttcttcaatc cctcatgcca tttgtagtcc atgaaacact gcgaaatctt tctggccagt
    21061 tccggcttga gatcatgcgg aattgaccag gacgaggtcg ggaaggtttc cgattgccag
    21121 atgaggcgca aggtatccgg tttgaccgca ccgcgcgcca ccatgcgctc gtagacgtcg
    21181 gaggcaaccg gcgccatgtc gtaatcgccc gaggcgacgc ccatcaccga cttgtcgtgg
    21241 ccgcccgaca tcagcacctt gtagtcgtcg tccggtttca ggccgagatc ggggaagaac
    21301 acgcggggag ccagattgcc ggaattggac gagggcgagg tatgggctac cttcctgccc
    21361 ttcagttcgg gcaaggactt gtaggggctg tcggacttga ccagcgcaaa cagcttgtag
    21421 ccggaaacgc ccttctctga tccgatgacg gcgaatggga cggcgccggc gagattgacg
    21481 gcaaaaccga ccacgccggt ggcaaaaccg gaaatatgca ggcgcccgga acgcatcgcc
    21541 tcgatctgcg ccgcattgtt ctgaaccgga taatagacga tcttcttgcc cgtgcagctg
    21601 cccagatgat ccatgaacgg ctgatagacc gtctggtaga cagccggatt ttcgaccggc
    21661 gaataggcga agatcagcgt cgacggatcc ttgaggcggg ccggatcggt cggcgtatcg
    21721 gcgaccagat cgttgttggc atcacaatag gcgttgtcca gctgcccgcg attggggcag
    21781 gaatcctggg caagggccgg cgcggcggca aaaaccgccg cggccatcat cagggcgcgc
    21841 accatggtcg acctcacggc ttgggagcgg ccggagcagc aggctgctgc gcggccttgg
    21901 cggcggcctc ggccgccttc ttggcggcgg catcggcttc gcgcttggat tcggcatcgt
    21961 aggccgcctt gttgtagggc gtgcccgact tttcagccac ttcgcgcacc accttccagt
    22021 ccttgccgta ggaaatcggc aggaagcgat cgtcgccgtt gaattccttg gtcatcgccg
    22081 gggtgaagcg gaacgaatag aaacattccc tcagcttcgc cgcgagctcc ggcttcaggt
    22141 catgcgcatg ggcgaaggat gaggtcggaa acaccggcga gcggtagatg atgcggaagt
    22201 cctcagcctt gatggtgccg cgcgtggcca tccggtcaaa cacgtcggag gcaaccgccg
    22261 ccatgtcata atcccccgaa ccgacaccga gcaccgactt gtcatggcca ccggacatca
    22321 gcggcttgta gtcatcatcg gttttcagac cctgttccgg gaacagcacg cggggagcca
    22381 gatttccgga attggaggag ggcgcggtat gggccacttt cttgcccttg agatcggcca
    22441 gggtcttgta ggggctcgat gccttgacca cggcgatcag attgtagccg cgcacttcat
    22501 cgcccagtcc cttggcggcg aaaggcacgg cgccggcaag gttgaccgca aacccggtcg
    22561 gcccggtgga aaacccggcg aaatgtagcc ggcctgaacg catcgcttca atttcggcgg
    22621 aattggactg aacgggatag tagaccgtct tcttgccaac gcacttttcg aggtgatcgg
    22681 tgaacggctt gaagatgttg gcatagaccg ccgggtcctc gacaggcgtg taggcccaaa
    22741 ccagtgtcga cggatcgcgc cacttcttct gatcggtcgg gacatcagca acaagatcgt
    22801 tgtcggcgtc acaatacaag atgtcgagct gaccacgatt ggcacaggcg tcctgcgcaa
    22861 ctgcaccggt gatccccaag gtccaagacg aaacggtcag cgccaaaccg gcgacgatgg
    22921 ctccacgcat tcttcttctc ctcccgcgcc ggctttcaaa ccggcctgtc atggggccaa
    22981 tgttggcctg caattcgaag ctgtcaacgc cggatcgcat ttcgccggcc aagaatgcga
    23041 ctgatcccac aactccctgc acgtcccagc cggcaggtca tgattgcatg gcaaggatga
    23101 tcttgccgga atgggcagag gattccatca gtgcatgggc ggcgcgcgcc tcttcgagcg
    23161 gaaacaccct gtcaatgacc ggccggatcg ttccggcatc cagcagcggc cagacctcgg
    23221 cccgcagccg ttcggcaatg gccgccttga gcgcgaccga acgcgggcgc agcgtcgatc
    23281 cggtcatggt caggcgacgc agcatgaccg gcatgaaatt gacctcggcc accggggttt
    23341 gaaggaaggc gatctgcaca agacggccct cgaccgccag actggagatg ttgcgctgga
    23401 tataggcccc gccaaccatg tcgagaatga cgtcaacgcc ctggccgccg gtcaggcgct
    23461 tgatctcggc gacgaaatcc tgcgtccggt aattgatcgc agccgtcgca ccgagcgaga
    23521 cgcaaaaagc gcatttctcg tctgttccgg cggtggcatg gaccgtggcg ccgaaggcgc
    23581 aggcaagctg gatcgccgtc gagccgatgc cggacgagcc gccatgaacc aggatcgtct
    23641 cgccggcggt caggcgcccc cgggtaacga cattgtcgta gacagtgaaa taggtctcgg
    23701 gaatgcccgc agcctcgatc atcgacaggc ctttgggcac cggcaggcaa agcgtcacat
    23761 cggcaatggc atagtctgca tagccgccgg aaatcgtcag tgcacagatc tcgcgcccga
    23821 tctgcgacgc tgacacccct tctccgaccg ccacgatgcg cccggcaatc tccagccccg
    23881 gaatgtcggt tgccccgggt ggcggagggt atttgccggc ccgttgcagg cagtcgggac
    23941 ggttgacccc cgccgcatga accgcaacca gcacctggcc agggccgggc tttggcacga
    24001 ccgcttctgc atcaaggaca atcacctcgg ggcccccagc cccggcaaag cggatctgcc
    24061 gcattctctg gcctgaattc atggaacaca tctgagcaac cctggttttc aaacggctga
    24121 tggccagcag atccatagcc gatcattcaa ccacggccca agcggtcaga tcaccgtggg
    24181 ggctcatgac gcagccagcc gagcctggct gctgcgcgac ggcgccagca acagatgcgt
    24241 ttatgaagtg cccattcccg cgatcattac gccaccagca tcacctggcc ttccactggc
    24301 caaaagcctc aaatagggcc cagtcggccg gatcggtctc cggcctgcaa gaaacggtca
    24361 gccagaacgg atggcgcagc gatatcagcc ggcatcttca gagcgatacg gttccagccc
    24421 ggccatttgc ccgactgtga ccggcttgac cggcgcccgc aggcgcagat gcccgatcgc
    24481 ctgcgcacta tgccccgact gctcggcgaa aagctccgtc agggtcagcc cgcagcttcg
    24541 cccctgacag ggccccatgc ccgcacggga gaacaccttc aactgattgg gaccctgcgc
    24601 gccttgccgg atgagccgac gcaggtctcc ggcagcgaca tcctcgcagc ggcagacgat
    24661 cacatcatca gccggaatgc ggaattgtga ggctggccga cagagcgcat cgagaaaggc
    24721 gcgcccgcgc tgagcccggt tcaggcgagc cacggcatct ctccgctgcc cctggcgggc
    24781 agcggtgccg gccgggtcga tgcggtcggc aatggccagt gcaccaagaa tgccgcgtgc
    24841 cgctgccgcc tcggcgccag cgataccggc gctatctccg gtgatgaaca ggcctcggat
    24901 gtccgtctcg ccagcggctg acagcgacgg ttcgaaagcc agtcgcactt tgttccacag
    24961 atgcgacacg ccagaggcca tggccagatt gatctggggc acgacccctt gatgcaacag
    25021 gagggtggcg gccgggatcg tgatcctgcg cccgccggcg acggcttcca cactggacag
    25081 ttggttttca cccttggctt ccagcgcgct gacattgcga atgaggcgat gagcagatag
    25141 cgcctcgtgc aacagtttca gccccttgaa cagataaggg ctcgccagaa agcccggcag
    25201 atgcggcaag gcaccaagcc agttgccgcg cggcgtagtg tccagaatgg ccgcgagttt
    25261 gataccagct tgcgcatatt gcgccgccag cagatagagc agcggtccgc agccggccag
    25321 cactgtcggc ccttccggaa cgatggcgct ggtcttgagc agggtctggg cggcacccgc
    25381 tgtcatcaca ccgggcaatg tccagcccgg aatcgggaac ggccgttcca gcgcgccgga
    25441 tgcgatcatg acatggcccg cctcgatcag gcatgcccga tccggcatgg caagactgac
    25501 ggtgaagccc cccgcatcct gccgctcgat catgaatacc gtggtccggc aaagatgctg
    25561 cgcaccgcaa gccgcaaaag cctcggcaag cggccgacct gcgcgatagt cggcgccgag
    25621 ccgatcttcc tttgccaggg ggccatccat gacggcacga tagacctgtc cgcccgcagc
    25681 cgggttctca tcggccacga caaccgacag tccggcacgg cgggcggcaa tcgctgcggc
    25741 catcccggca gggccggccc ccacgatcag gagatcgcac gtcatcactg ccgctccgca
    25801 agtagatcag gcgccgccgc cccggtgacc acctgcatgc cgtcacggac cggaatcagg
    25861 caggcctggc ggttcggctc gccatcgatt gtcaccaggc attcgaaaca cacgcccatt
    25921 gcacagaacg gaccgcgcgg tgatgctgac gtcgggctgc ggcgaaagcg cgtgatcccg
    25981 ttggccagga gcgccgcagc aacggtgtcg ccttcggcag ccatcacggc ctggccatca
    26041 acggtgatgg ttacccgggc aaccggttca agcagccgcc ggaacatcgc cgaacctcct
    26101 tgcgctgaag gccgctatgg cttccggcca gatatcgctg gcaatcgcct cggccatcac
    26161 cgaggcatga gccgctgcca gcgtcacccc gctatggcag gtgacaagat aggcacccgg
    26221 agagccggtc gagcgatcat agaccggata gccatcaggg ctcatcaccc gcagggcccc
    26281 ccagcttcta acgatctgcg cacgggcaag cagggggaat accttcaccg catgcgcggc
    26341 gatctgcccc agaacctggt gggaattgcc ggtgtcaaag ccgacctcct ccttggaatc
    26401 tccgagcatg acgccgcctt cgtcagtctg gcggatcgtc ccgagcggat agtgcaggaa
    26461 aggagcgagc ttctccgtca cgatcacctg gccgcgttgc ggccgcaccg gcgccgaaag
    26521 cccgaccatc ggagcaagcg tcgcattgcc aagaccagcg gccagcacaa ccttgcgggc
    26581 ccggaactct ccggccgagg tcacaatgcg aaacccgtcg gctgcgggca tcagcgattc
    26641 gaccgtagca ttcgggcgat aggcgatacc gcgccctatg caggcctggt gcaaggcacg
    26701 gaacaggcgc aatgcgttca catgtccatc aagccggcaa taggtgccac ccacaacctc
    26761 cgagccgatt tcggggagca aggctttcat ctgttgatga tcaagcatct cgacagtggc
    26821 ggaatccggg gccgcgttat gcagccgctg caattgcgcc gcacgttcct cctgctcctc
    26881 ctcgctcagg caggccatga ggccgccgcg ctgttcgagc gcgacattga tgccgctgtc
    26941 gcgctggagc gtctcggcga accccttcca ggcagaggct gatccgagcg tccagcgcgc
    27001 atagtccggc agatcaatgc ccttgccctg aacccagacc agcgcgaaat tgccccgtga
    27061 cgccctgtag gccacatcgc cttcgtcgag cagcgtcacc gcctgccccc gcatggccag
    27121 cccataggcc accgacagcc cgaccacgcc gccgccgatc acggcaatgt cgcagtcatt
    27181 tccgcaccgg gacaggtcgg tcatgctcaa ttccgttcat ccccgacaaa aagccgttcg
    27241 agtccgtaga gacgatcgag aaccagcatc agaacaaccg tgcccaggat cagcaatgct
    27301 gaaatggcgc agaccagagg gtcgatcgaa tcctgaatgt agttgaacat ccggaccggc
    27361 aaggtggtgg tcgtgggtga ggcgacgaac accgtcattg tcacctcatc aaagctctgg
    27421 atgaaggaga gcgcaaaccc gctggcaagg cccggcacca tgaggggcaa gatgacgcgg
    27481 cgatagacga tggagcggga ggcgcccagc gagaccgcgg catcttctat ccgcttgtcc
    27541 atgccggccg cgctcgccag aaccatccgc agcgcaaagg gaaagacaac aatgacatgc
    27601 gcaatcacca gaccctgggt cgtgccggac aggccgacca gcgagaagaa ccgcaggaaa
    27661 gcgatcccga gaacgatatg cgggatcatc agcggcgaca tgaagagcgc cgcaagcgca
    27721 tcacggccgg caaaacgata gcgtgcgatc gcgatggctg ccggtgtggc aaaggcaagc
    27781 gcgatcatcg aggagagggt tgcgatcaca aggcttgtct ggaaggcccg agagaattcc
    27841 ggatactcgg cgatcctgcg gaaccagcgc agcgagaagc cgttggtcgg cagcgccaga
    27901 aatccttccg gcgtgaacgc cacgaggcag acgacgacca tcggcgccag caggaacgtc
    27961 gcgaacagag tgtggaagat cagcgcaagg ggcccgttct tcatgacgca aacacctgcg
    28021 cgtagcgccg ctcaaccacc ctgttccagc cgacaaccac ggcgacgatg ccgacgagga
    28081 gaagcaccgc cagggcagcc cccagcggcc agttcaatgt gttgagaaac tcgtcatagg
    28141 ccagggtcga gaccactttc agacggcgcc cgccgatgat ggccggcgtc gcaaaagcgc
    28201 tcgctgccag cgagaacacg acgatgctgc cggacaggat cccgggcatg gcctgcggca
    28261 gaacgatgcg gcggaagatc gtcgcctgcg acgccccaag cgactgggcc gcccgctcga
    28321 ccgccggatc catccgcgac agcgccgccc agacggacag gaccatgaag ggcacaagca
    28381 catgaaccag cgcgatgacc acgccggcct cggtgaacat cagcgacacc gggccggagg
    28441 taagccccag cgtctgcagg gccgttgaaa ccagcccttt gccgccgagc agaagcgccc
    28501 agccgagcgt gcgggccaca actgacagaa gcagcggacc gatgaccacc aggatgaaga
    28561 cggagcgcca gggttcgcgc atgcgtcgaa ggatataggc ttccggcgca ccgatcagga
    28621 cacagatgac ggtgacccag agcgaaatgc gcaaggtccg caggaagatt tcggcaaagt
    28681 agctgtcgga gaatatctcg acgaagttct tcgccgtgat caccggcacg atgccctgag
    28741 agctgttcca gccgttaagg gacagcagaa aggtcatcgc gagcggtgtc gcaacgacgc
    28801 cgaggaagag tagggtcgcc ggcccggcca gagcaaaggc atgccggtcc cagggggtca
    28861 ttgcgctgcc tcccgtgccg tgaccagcgc atcctgcggg cgaatgacca gcgacaccgt
    28921 gtcgccgatg ccgggcaggc ccatcccgtc attctggcga atgaccagtg cggggccgcc
    28981 agccgtcgca atctggaaca gccagtgatt accctggaag atgcgagcct cgacccgacc
    29041 gggaaggccc gccgcgtccg ccgacgccag ctgcagcttc tcgggccgga cggcgagcgt
    29101 gcaaggaccg gcaacgctgc gtcccgtcgg aatggtatgg ccggccacaa tcacggtctg
    29161 gccaagtcct tctccagcaa agacattggt cttgcccagg aacgaggcca cgaaggcgga
    29221 ttgggggtgt tcatagacat cgaaaggcgc cccgatctgt tcgaccttgc ctgcactcat
    29281 cacaacaatc cggtcggaca gggccagggc ttcatgctgg tcatgcgtga cgaggatcgt
    29341 cgtcgtcccg gcccgctgct ggatctggcg caactcgacc tgcatctcct cacgcatctt
    29401 ggcatcaagg ttggacagcg gctcgtcgag caagagaatg gatgggcgaa tgacgagcgc
    29461 acgcgccagg gccacacgct gctgctgccc gcccgacatc tggcgcgggt aacgctcgcc
    29521 cttgtcggag agcccgacga gcgccaggac atccgtgacg cgctggccga tctcggattt
    29581 ggcaatgccg cgcatctcaa ggccgaaggc gacattctgc gcaacggtca tgtgagggaa
    29641 aagggcatag ctctgaaaga caatgccaag gccacgcttc tctggcggca gtcttacgag
    29701 atccgcgccg tcaagccgga ttgcgccggc agtcggctcg atgaagccgg cgatcatctg
    29761 gagcgttgtc gtcttgccac agcccgaggg gccgagcagg gacacgaact cacccttggc
    29821 aacagcaaga tcgagccgct ccaccgcgag cacctcgcca taggccttct gcagcccctc
    29881 aagcacaaga tagctcacgg ctcccccttc gcagcgcaag ccgctctctg gtccatccgg
    29941 aaagtggtcc ctgcggcggt ggcccggcca agccaggcca cgcaggatgt cagcgctcga
    30001 tctcgcggac ccagcgcttg ttccattcat cgcgaaccgg gttgattgtg tcccagtctg
    30061 ccgccacaag tttctttgcc agatcaccga ccggcatcat cccggctttc tgcaccttgg
    30121 catccttgtt gaccggcgca aagccagcat gatcggcgag aatggattga gcttcggccg
    30181 agagcatgtg ctggacgaaa gcatgtgcct tgggcgagat tgtcggcttg gccacggcgc
    30241 aaagagcagt catcacgacg ggcgcacctt cggcaggata gacgaactcc accgggacgc
    30301 ctgcctcggc cagagactgc acacgcgcgg acccccagac gccaaagatt gcctgaccgg
    30361 tctggaacag ctcggcgtgt tttgctggag agggctcata ggcgaggata ctcggcccga
    30421 cagcgtcctt catcaccttg aagccggggt cgatgttctt ctcgcccccg ccattcatgc
    30481 gggcgagcat caccaccgtg agcaggccat agccgttgtt gagtggcggc atgaccacct
    30541 tgcccttgta tttcgggtct ttcagatcat tccaggaggt cggcggcgcc catccattct
    30601 ccttgaacac cttggtgttg tacatcaggc cggtcgcgat cagccccagc cccatcgcct
    30661 tgccgcccgg cagcttggcg acatcgtgga tcttcgactg atcgacaccc tgcaggggcg
    30721 cgcagaagcc cagcgagacc gcccgcatca tcgggccgtc atcaatcagc gcgacatcga
    30781 tttcctgctt gtccttgccg gcctgcagtt tggctagggt gtctgtcgag ttgccggcca
    30841 cataggtgat cttgacattg tgggccttct cgaagggcgt gatgatctgt tcgcggatga
    30901 tcttttccgt cgatccgcca tatccggcaa ccagaagcgc ttcctgggcc agagccggcg
    30961 ccgtggcggc catcgccaga agcgaggctc caatgaacag tttcaacctg ctggacatac
    31021 gacttcctcc cgttcgtcgg catgatcgcc cgaccttggc ccggatagtg aagggcttct
    31081 atcatcagat caaattcaaa attgacagct cttcatgcat aaatattatt gaatgatgtc
    31141 caatctgagc cgccagctcg atgcgtttcg cgccgtcatg ctgacaggag gcatgaccgc
    31201 cgcagcgcag accctgttca tcacgcaacc agcggtaagc cgcctgatca aggatctcga
    31261 gcatgacttc gggctccggc tgttcgagcg ccacggcaat cagatcgccg caacggcgga
    31321 agcgcgcagg ttgtttgagg aggtggagcg gacctactcc ggcctttccc atctgcgggc
    31381 cttcgccgat gatctgcgcg gttcgcgcgt cggctcgctc cggattgccg ccttgccggc
    31441 cctggccatc gcattcctgc cattctgcct tgcgcgcttc cggaaccagc ggccgaatct
    31501 gtcgatcatg ctggatggca taccgtccca cctcgttctg gagcgcgttg ccagcggcct
    31561 gttcgatatc ggctttgcag ctgtcccggc cgagcggccc agcctcaggc tgacccctgt
    31621 tccggggtca ttgatggtgg tgatgcctgc gggacatcgt cttgcgggcc ggacggtact
    31681 gcgcgcgaag gatattgcag gcgagagcgt tatcatgctc ggacgcggtt cctatctgcg
    31741 ccattccatc gagacggcac ttgcgcaggt tcatgttgag acgcagatcg aatcgccgct
    31801 tgcggccatt gcctgcgcct ttgttgcgca aggtctcggc attaccctgg ttgatccatt
    31861 caccgctgcg gatttcctcg gccgcggcgt ggtcgcgcgt cgcttcgagc cggcaatcga
    31921 cgtcggatac tcactcatca cttctaaaga caggcccttg tcgcaagttg ccaatgattt
    31981 tgtcgccagt ctcggtgagg cgacagccgc ctacctggag gaagcgcagt ccagataggc
    32041 attcttcgcc tgcctgcgcc cttcaccagc gcaacagccg cgatccgagt gcggcgccgg
    32101 cgccggtcac gatcaatgtg gcaatggaat accagaaggc gacgaagagc gggctgtcct
    32161 ccatgcagtt gagcgcatag aaactcgcgc caattccggc cgcagcgagc cctgccagcg
    32221 ctcctgctcc ggcaggcgag gccggcatgc cgtgccgcgc cgccgcaaga aaccccgcca
    32281 gcggaatgct tgagagcgcc gggatcagca caaggcagtg gctggatttc gtgccgacca
    32341 gacgcgtcat ggctcccgcc gagctgttgg tgacgagatc catgacgaca aaggccaatg
    32401 cgaaaaccgg cacaatcgcc agcaccgaca gtctgccggc aaggttgaga tggacgctca
    32461 tcgccagcag cagcatgagc gcaatggtgg ccaggctcag cgtgatcgac aatttcatcg
    32521 cgatggccgg catcgcctct gtggcatccg ggcgcaggcc aatcgcgcta cccatgacga
    32581 tggcaaggac aacaacggcg agcggcacga cgcgcagcag cgacgaacgc gcattgcccg
    32641 ccggcgtcgt gtcggcggca agcgcattga tcaggtcagc ggtcctcact gtgcttcccc
    32701 tcgtgcgaaa gccgccagtt tcttgagcgc ccgatgcagc gccacgcgca cagctccttc
    32761 ctgcatgccc agcgaccggg acacctccag cgtcgggcgt tcctcaatcg ctatggcgcg
    32821 gacaatggcc cggtcccgct cgcccagccg cgaaagccag tgctcggcct cgcgggcatc
    32881 gtcggctgcc ggcgcggggg ctgcttccag ttcgtgctcg acatcctcca gcggcacggc
    32941 aacggtccgg ccgcgccggc gataggcgtc gatgatcttg tgccgggcaa ccccgaagag
    33001 ccacggccgg aagggctgtg accggtccca gttgccacgc ttgagatgca cagcaagcaa
    33061 gatgtcctgc acaatatcct ccgtttcgcc gccgccatgt tgggctgcgg agcggcgcac
    33121 aatggcgcga agcactgggg tcatcgccat gagaagctgc cgatatgtcg cctcgtcgcc
    33181 cgccagcgcc cggcccatca tatcgcccca caggtctgat ccacgctcgg ccacaacttg
    33241 ccctgttcgt ctgtcagcgc gcttacgtta cagcccgcag caaaaaaatc gccagacagt
    33301 ctgtgctgcg gcgatgcggt tcacaagaac gtgagcgccg taacggacgc ctcgatcgct
    33361 gcgaattgcc caatgtcccg cccggagtgg ggaaaccatg actgaggaga atatcatgga
    33421 tcgtcgtatg ctgtcgctgg cccttgccgg cgccctgacc accgcgctca ccgcaggcgc
    33481 gacaccggcc gccgcacagg gcatggagaa gtgctacggc attgctctca aaggccagaa
    33541 tgactgcaag gcgggcgctg ggaccacctg cgccggcacc tcgaaggtcg actaccaggg
    33601 cgacgcgtgg aagaacgttc cggccggcac ctgcgagaag accgcttcca agtcctccaa
    33661 gaccggcttc ggcatgctca aggagttcaa gagctgatgc cgcgcgctgc cggatattcc
    33721 cctgtagctg tgctcgcgag accttcggca gcgccccatt cccctcgtgc cggcctgccg
    33781 gcacgagcgg ggatcggcct caagtccgaa cattcagagg agctgcgcaa ggggccttca
    33841 tcgattggtt tcctcgaggt ccatgcggaa aactacatgg gcgcgggcgg tcaaccccat
    33901 gccgacctga cggccttgcg ggccctctat cccctctcgg tgcacggggt gggcctgtcg
    33961 atcgggagcg aacagccgct tgatgcgacg catcttgatc ggctccgccg tgtgctggag
    34021 cgataccagc ctgaaagctt ttccgagcac cttgcctggt cgacccacga cggacactac
    34081 ctcgccgatc ttctgccggt cgcctacaat gaagcgacgc ttgtgcgcgt ggtggatcat
    34141 atcgatcagg tccagaccta tcttgggcga cgcatgctgc ttgagaaccc ctcgacctat
    34201 gtcgcctttt cggcatcgga ctgggacgag gtggatttca tcgccgaagt gcagcggcgc
    34261 agcggctgcg gcctgctgct ggatgtcaac aacgttgccg tgacctgcgc caaccatggc
    34321 cgcgatccga ttgcctatct cgatgcattt cccgtcgctg ccgtcggcga aattcatctt
    34381 gccggctacg cggaggattg cgattcgctc ggtgcccgat tgctgattga cgcacacggc
    34441 tcgcccgtcg cgaccgatgt ctggcagctc tatgcccatg cattgacccg caccggcccc
    34501 gttgcgacgc tgatcgaatg ggacaacaac gttccagcgc tcgaccgact gattgccgaa
    34561 gcaacgatgg cggaaggtta tctgtccgct gccgcacaac gtcgggacag cgccgcatga
    34621 gccaggccac ttttgttacc gcactgctgg atcgggacaa gccggttccg gacggtttgc
    34681 gcacatggaa cggctctgat ccgcttcagc gcttccgtgt ctatcgcaat aacgtcgcgg
    34741 tggcgctgat tgacactctg gcggcaacct ttccggtaac gcttgcactc tgcggcgagg
    34801 agttctttcg cgcaatgaca cgcgcgttcg ccctgggctc gccgccggct tcaccgctgc
    34861 tggcggattt cggccaggat ttgccggatt tcattgcggg ctacgctgcg gcgcaaaagg
    34921 tgccctatct cgccgatgtg gcacgcctcg aggcggcgcg tgctttggtc tggcatgccg
    34981 gggacgcggc gccgcttgct gctgagacct ttgcctgctg gcaggatcgg atcggtgaag
    35041 cggtgctgac cgttcacccg gcccacgccg tccttgtctc gccctatccg gtggtctcga
    35101 tctgggcggc gcacaatggc ctcggcgatc tagaggacat cgatctcagc cagcccgaag
    35161 aggcactggt tgtccgcccg aacctcgaga ccgagatctg gcgcctgccg cccgggggcg
    35221 cggcatttgt ccgcgcgctc gaagctggcc agagcgttgc cgacgccgcg atgaatactc
    35281 tggcagacac cccggctttc gaaccggcga tggcgctgtc gctggtcatt cgcgcccgcc
    35341 ttgtcactgc gatccattcc ggagcctgcc aatgacccgc attgcgtccc ttgtccgccc
    35401 agtgattgcc gcgctggaag cgcttcccta tgccgttctg gcccttctgg cccgcttctc
    35461 gattgcggcg gtattctgga agtctggcca gaccaagatc gagggtcttg cgatcgatct
    35521 cattcaaggc aaggttgagc ttggcctgcc gcgcgtcacg gcctcggcga ttgacctgtt
    35581 ccgcgaggaa tacaagcttc ccctgctacc gcccgaactg gccgcctatc tcgccgcagc
    35641 ggccgagcat gtgtttcctg tgctgatcct gatcggcctt gccacgcgcc tttcggccct
    35701 tgctttggcg atcatgacgc tggtcatcca gatcttcgtc tatcctgatg cctatgcact
    35761 gcatggcacc tggctggcgg tcctgctgat gctggccaag ttcggaccgg gatcgctgtc
    35821 ggtggatgct gccatcgcca atcgttccac atagtgtcta ttgccgctga gaagtaacat
    35881 attccgactt cagggccgag aattgccggc gaattcgggg gctgaagagg ctgaattctt
    35941 ggctttgtga gagcgccatt ctgaaggcac ctccggcaag ccccggaagt atcgaggtaa
    36001 ttttctcaca ttagagcatt ttcaagcgaa gtggagcccg gttcgcgcaa agaaaatgcg
    36061 acaaaa
//
LOCUS       DAOMXG010000005        34944 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_836_length_34944_cov_11.978589, whole genome shotgun sequence.
ACCESSION   DAOMXG010000005 DAOMXG010000000
VERSION     DAOMXG010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 34944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34944
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_836_length_34944_cov_11.978589"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..770)
                     /locus_tag="PLQ11_00775"
     CDS             complement(117..770)
                     /locus_tag="PLQ11_00775"
                     /inference="COORDINATES: protein motif:HMM:NF017985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1007 family protein"
                     /protein_id="HRK23463.1"
                     /translation="MNARRSGPLCGWTLAAALIAAAPAAAHPHVMVTARSALVMDAGK
                     VTGVRHSWIFDEAYSAYATMGFPKEKDGSFARRELDDLARLNVESMGEFKYFTSLKQG
                     RTELEFAAPPPGYRLEHNGKALVLHFLLPLKTPVVADSSIALRVDDESFFVAFSFAED
                     NPVMIEGNPGSCKLEMKKPQKSLGSADLSRLSEDMFANLKSGFTDQFATTIRLACAK"
     gene            complement(890..1138)
                     /locus_tag="PLQ11_00780"
     CDS             complement(890..1138)
                     /locus_tag="PLQ11_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23464.1"
                     /translation="MVISKDLFLAILAMDSYNRGYLPGVVLPGSAATGIGNATLRTDA
                     QPQDYEAASFFAQAYTLTGSYADASGGPGLSAGQTVIS"
     gene            complement(1143..1934)
                     /locus_tag="PLQ11_00785"
     CDS             complement(1143..1934)
                     /locus_tag="PLQ11_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23465.1"
                     /translation="MKALFWSLGLLIGLPILALAGWIGVGWVYMRATEVDHRFRLTVE
                     ITTPDGVRSASSVINVNRTDIKWMPLSGIANRFNHTITGEAVFVDFGSGRHAIGLLAH
                     GPRAEDSDTMITLWVQAYAGFMQRGNEDIWRGRRKLEGIVELAPELVPTIVTFTDLAD
                     PASAKVVYATGRDIRFVPDSPGSSSGAYGDFGPLVIDRFEETFGKGYALKRVTIEMTT
                     DPITTGIEGKINWIGDYAKETTFERLLRGNAGGSSLMPGRNLKRS"
     gene            2382..5876
                     /locus_tag="PLQ11_00790"
     CDS             2382..5876
                     /locus_tag="PLQ11_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HRK23466.1"
                     /translation="MELPLALRQVSLADKYDLSKNEVFLSGTQAIVRMLLMQRERDRR
                     AGLNTAGFVSGYRGSPLGGLDQQLIRARREIAAAPIRFEPGLNEDLAATACWGTQQAE
                     MRGEGRYDGVFALWYGKGPGVDRTGDVFRHANMAGTSRHGGVIALAGDDHTAESSTNA
                     HQTEFNFVDTMIPILNPAGVQEILDYGQHAYAMSRFAGVWVALKAVKDNVESTASVDG
                     SLERVSILTPGNYPMPDGGLNIRPRDNFLEQERRLHEFKREAALAYVRANGLNQMVFS
                     GGAKPRIGIITVGKSYLDTRAALASLGIDAGRAQTLGIRLFKIACPWPIDPEELKDFA
                     RGLDTIMVVEEKRSLIEVQVREELYGSANQPVVVGKRDEGGQWLFPVTAALDPNDIAI
                     AIGERLIARQPNEEIRARIAGLRQAQTGLARIAEVAARVPYFCSGCPHNSSTVVPEGA
                     RAYAGIGCHYMVQWMDRETEGFTQMGGEGANWIGESTFSTRNHVFQNLGDGTYNHSGS
                     LAIRWAIAAKTNITYKILFNDAVAMTGGQTHEGALTPWRIAQQVRAEGVERIALVSDD
                     PYKYGAEAKWPPGVSFHHRSELMPVQKELAAIPGVTVMIYDQTCAAEKRRRRKRGLYP
                     DPDKRVIINELVCEGCGDCGKKSNCVSVQPLETEYGRKRRIDQSNCNKDFSCLEGFCP
                     SFVTIEGARLKKAASIAGMAAIPSVPEPEFRDLDHDPWSCIVTGVGGTGVVTVGAILG
                     MAAHLEGRGCGMIDMAGLAQKGGAVFSHIRLARSPEDIHAIRVEAGSADLILGCDLVV
                     SGSRKVLAAMRQGETVAVVNTAEVASGEFTRKPDYQLPAERLKRSIREAAGEGRADFV
                     DATAMAARLFGNTLAANMFMLGYAFQRGAVPLVSAAIEQAIRLNQESVEMNLAAFRWG
                     RVALHDMASIRRLIDETIPPTRQETFSQGLDGLIERRMEFLTNYQNAGYALAYRRRVE
                     VIRAAEEARMPGSTRLTEAVARSYAKLLSYKDEYEVARLYTSGSFLKQVNAIFEGDMR
                     FTFHMAPPLLNRRHPSTGEPMKSSFGPWMMRALKVLARLKGLRGTAFDPFGRTAERRT
                     ERQLIADYEVLLDEFSRSLGPGNHAVAVELASLPEQIRGFGHVKDRHIEAARREHERL
                     MQRYRAGGDGPPLRAAAE"
     gene            5879..6250
                     /locus_tag="PLQ11_00795"
     CDS             5879..6250
                     /locus_tag="PLQ11_00795"
                     /inference="COORDINATES: protein motif:HMM:NF016854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HRK23467.1"
                     /translation="MSPADLEDFFAPFAKVNVRRMFSGHGIYVGTACFAISAFGTIWL
                     KADADTEAAFAAAGCRPFSMTTPDGSTRTIRSFWSLPEPALDDAEALKRWCGPALRAA
                     QATALEHAAKKWKRVFAKSML"
     gene            6545..7039
                     /locus_tag="PLQ11_00800"
     CDS             6545..7039
                     /locus_tag="PLQ11_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23468.1"
                     /translation="MRRTLQIVLLALSTSALAQSPGGHTGHGGMAAQGFVLPDDPRTA
                     VEIPPDVRAFLRYEMRGHLEQLATLMARIGEGNFKQAAAFARSGLGVMGNHPPGAPNP
                     AQFVPPEFRMMGQAMHRAANAVAEAGEAAVTPPSAADWKTMAAAAANLSAACAGCHGS
                     FRLR"
     gene            7111..7785
                     /locus_tag="PLQ11_00805"
     CDS             7111..7785
                     /locus_tag="PLQ11_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRK23469.1"
                     /translation="MIACDALARLAHWSGDLTPDERSRAARGVTEKTFAKGTYICHLG
                     DRLDCWVGVSEGLLKLSTVTRNGKAMTFAGIAEGGWFGEGSLLKNEPRRYDVVALRDC
                     RLALLDRATFLWLFEHSVGFNRFLVRHFNERLSQFMAQVEFDRTLNATERLARTIVWL
                     FNPVFYPKARSHIRISQEEIGLLCGLSRQSANQSLQALEAAGLIAMEAGGMTVRDLAR
                     LQRFET"
     gene            7931..9898
                     /locus_tag="PLQ11_00810"
     CDS             7931..9898
                     /locus_tag="PLQ11_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRK23470.1"
                     /translation="MSDTTAASRDTFPKWLAHHARVRGSRPAMRFKEAGIWQRWTWAE
                     IFDIVRDYAVGLTDLGLKRGDKIAIVGTNRPKLYWSIMAAQCLGAVPVPVYADAVADE
                     MAYVLDHAEVVMAAVEDQEQVDKLISVRDRVPRLRHILYDEPRGLRDYDHSHLHPISA
                     IIARGKVMQQEGPDVRGAIDRSIAAGKADDLSIMLYTSGTTGRSKGVMLSAGGSVNAC
                     SDTVKFDKLSDTDEVIAYLPLAWVGDHYLNYGQALVAGFCVNCPESPETAVDDRREIG
                     ATFFFAPPRVFEGMLTRIMIRMEDAGPLKRRMFHHFIGVAKQWGEKILNGETVPLGAR
                     LHYQLGEWLVYGPLKNVMGFSKMRVGYTAGEAIGPELFSFYRSIGLNLKQLYGQTEAF
                     LYVTCQPDGQIRADTVGPPAPNVDIKISEDGELLYKSPGMFQGYFKEPEKTAETMTPD
                     GYVRTGDSGFYDKTGHIRIIDRAKDVGKLASGALFAPKYIENKLKFFPNIKEAVAFGQ
                     GKDFVTVFVNIDMTAMGNWAERHNVAYASYQELANHPDVYKIIAGHMDEINKSLAAEP
                     MMARAQIARFLILPKELDADDGELTRTQKVRRGFIAERYEPLVTALYDGSKEADISVQ
                     VTFEDGRKGSISGRVRVADLNCYPVIEEKAA"
     gene            9895..10689
                     /locus_tag="PLQ11_00815"
     CDS             9895..10689
                     /locus_tag="PLQ11_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRK23471.1"
                     /translation="MSPQAGIERGDVLLAVDNVSLRFGGVKAITDVSFDIRKGEIRAI
                     IGPNGAGKTSMLNVINGFYHPQEGAITYKGVKREKMRPYVAASQGIARTFQNVALFKG
                     MTTLDNIMVGRTLKMRTPFFWQVWRHGPAMREEVEHRKFVEEIIDFLEIEHIRKVPVG
                     KLPYGLQKRVELGRALAMEPELLLLDEPMAGMNLEEKEDMCRFILDVSSQYGTTIALI
                     EHDMGVVMDLSDRVVVLEYGRKIADGTPAEVKADPTVIAAYLGVGH"
     gene            10700..11650
                     /locus_tag="PLQ11_00820"
     CDS             10700..11650
                     /locus_tag="PLQ11_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRK23472.1"
                     /translation="MLYNVFIDPFLQMVQAPDLLVQTLWEGLVAGVLYALIALGFVLI
                     FKASGVFNFAQGIMVVFAALTLVGLYEKGVPALLALILTIGVMYVLAWSTERFVLRPL
                     VNQPDIILFMATFGLTYVLIGLGEFIFGGNTKTMITEQLFLPKGAIDVQMLGGRVTFQ
                     KLDIAAAVIAGAMVAGLAAFFQYTRIGRALRAVADSHKAALSVGISLNQIWVIVWFTA
                     GIVALVTGIMWGARSDVSFGLQIIALKALPVLILGGFTSVPGAIVGGLIIGIGEKLGE
                     FYWGPLVGGGIESWLAFLIALAFLLFRPQGLFGDKIIERI"
     gene            11664..12740
                     /locus_tag="PLQ11_00825"
     CDS             11664..12740
                     /locus_tag="PLQ11_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009467584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRK23473.1"
                     /translation="MFYREAGQFKSTYESDMAVFPIRQDLIGVLAIIAVAYVLIPMTG
                     KFFIGTVMIPFLVFVMASIGLNILTGYTGLLSLGTGAFMGVGAYACYKLTTIFPGVNI
                     LIWIVVSGFFSAAIGVLFGLPSLRIKGFYLAVATLAAQFFLQWCFVRIPWLFNYNASG
                     AIEVPARMVLDIPVTGPNATELTRYYVLLTMVVFFTWIASNLVRGRYGRQWMAIRDMD
                     IAAELMGIRPLQAKLLSFAVSSFYCGVAGAMMMFLWYGGGEASDAFNINLSFNILFMV
                     IIGGLGSMIGCFFGAAMLHLVPIFLKFGLPAIGFPIGGATAEHLTHLIIGVLIIFFLI
                     VEPHGLARLWATGKQKLRMWPFPY"
     gene            12864..14183
                     /locus_tag="PLQ11_00830"
     CDS             12864..14183
                     /locus_tag="PLQ11_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009758035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRK23474.1"
                     /translation="MSLKKLALGLAAATAITTFAGAAQAQDSIYVPLLTYRTGPFASS
                     GVPIADGMHDYLRMLNERDGGIGGVRLTIEECETGYDTKKGVECYEATKGKKPAMYNP
                     YSTGITLQLIPKAGVDKVPVLSMAYGLSAAAVGNTFPWIFNPPVTYWDGASVFIKHII
                     EKEGGADKLKGKKIGLLHLDAPFGKEPIPLLENLAKEYGFELKLYPVPADKMQDQSSQ
                     WLAIRRDRTDWIYNQGWGAMNPTAVKEAVKNGFPMDKMVGVWWAGGDDDARAGEAGGK
                     GYKSLNFNGVGTEFGVMKDILKHVVDKGLSKYPKDKVGDNLYSRGVYNAMLIAEGIRT
                     AQAVTGKKAVVGEDVRRGLESLNITEARLKEMGMEGFAAPVKIACNDHNGHNKVYVAQ
                     WDGTKWTKASDWMSPMTDKVRPLLEAAAKDYAEKNNWPARTEACDAK"
     gene            14275..15117
                     /locus_tag="PLQ11_00835"
     CDS             14275..15117
                     /locus_tag="PLQ11_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009756455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRK23475.1"
                     /translation="MSAAAAAPAIEPAADTILSVNNIEVIYDHVILVLKGVSLTVPRG
                     GIVALLGANGAGKTTTLKAVSNLLQAERGDVTKGSIVFEGERIDQLTPNELVKRGCIQ
                     VMEGRHCFGHLTIEENLLTGAFTRRDGARAIKDDLEKIYNYFPRLRQRKASMAGYTSG
                     GEQQMCAIGRAMMSRPKMILLDEPSMGLAPQVVEEIFEIVKDLNAKENVSFLLAEQNT
                     NQALKYATYGYILETGRVVMDGEAKALRENEDVKEFYLGVGEGSRKSFRDVKSYKRRK
                     RWLA"
     gene            15228..16460
                     /locus_tag="PLQ11_00840"
     CDS             15228..16460
                     /locus_tag="PLQ11_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRK23476.1"
                     /translation="MAQFLDKLETRDPEKREKALFRDLRDLLVYAAPRAPTLRKQLRG
                     IDVGKLKGRDDLAKVPVIRKTDLKAMQQALPPFAGLAAARRGDLLRILVSPGPIFEPE
                     GRGIDWWGAARAFHAAGFRKGDLVHNCFSYHLTPGGHIMESGARALGCPVIPAGVGNT
                     EQQLEAIAGLKPDGYAGTPDYLKMLLDKGSEAGIDMGSLRKALVSGAAFPPSLQKEIA
                     GRGIEAYQAYATAELGIIAYETPARAGLVVNEDLIVEIVRPGTGEPVAEGEIGEVVVT
                     RLNGDYPLLRFGTGDMSKVLAGASPCGRTNMRLAGWLGRADQTTKVKGMFVHPGQVNE
                     IGKRHPELGRLRLVVTRANEQDVMTLKAETSSTDIGLAARVAETLNAVSKLKGQVDLV
                     AVGGLPQDGKVIADDRPI"
     gene            complement(16519..16836)
                     /locus_tag="PLQ11_00845"
     CDS             complement(16519..16836)
                     /locus_tag="PLQ11_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23477.1"
                     /translation="MKKIALGLLLAGLAATAAHSADQRLLNAEIANLVAGKQVFMGGG
                     YANFGKDRSFNFSGLFTGKWRATNRQICLVYDLGGTNCYPVVRNGKAYFILDNAGNRF
                     PVK"
     gene            complement(17023..18573)
                     /locus_tag="PLQ11_00850"
     CDS             complement(17023..18573)
                     /locus_tag="PLQ11_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HRK23478.1"
                     /translation="MTELTRRDTFRIAVAAGLASATVVASSAEEALAQAAAQKATPTF
                     RLLLVNDIYKMSGSAPGRGGFARLAAIARTERATGVPMIYAHAGDMYSPSLMSGFDQG
                     AHTVELLNVVPPDVFVPGNHEFDFGKEVYLKRRAEMKAPFLAANLRNEDGAPLAGHED
                     RRMFEMGPVKVGVFGVALATTPLMSSAAGFRFTDEMAAVQEQAKALREAGADIVVAVT
                     HTDMTTDFNIVRSRHVDVLLTGHDHDLRIYYDNRVAMVESGEEGDYVTIVDMYCTIAE
                     KDGKRTVTWRPVFRPIDSSHVTADPEADAIVKKYEAELSRELDVVIGKTAVDLDSRSA
                     VVRSQEAAIGNLMADAIRASTGAEVAVINGGGIRANKQYPAGATLTRRDVLTELPFGN
                     STVMVEVAGKDLKEALENGLSQVDNRAGRFPQISGIKLVYDPKAPAGNRIASIEVDGK
                     PLDPDKRYKVASNDFMYGGGDGYSALGRGRTLIGKTDGTLMANVVMAYIRKLGTVESK
                     VEGRITAR"
     gene            18711..19835
                     /gene="hemH"
                     /locus_tag="PLQ11_00855"
     CDS             18711..19835
                     /gene="hemH"
                     /locus_tag="PLQ11_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012253087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HRK23479.1"
                     /translation="MSQPVAPHLFRPSGHPDVPYGKIGVLLLNLGTPDGTDYWSMRRY
                     LKEFLSDKRVIEEPRWKWWPILNLIILTVRPGKKGKDYATIWNNEKNEGPLKTITREQ
                     AEQLAGRLAGYPNIIVDWAMRYANPTTESRIRALQEQGCERILLVPLYPQYAAATTAT
                     AADHAFRALMKLRWQPAVRVAPAYHDDPVYIDALARSIRAHLATLDFEPEVLLATFHG
                     MPKKYHLAGDPYHCHCMKTGRLLREALGWDKDRYVISFQSRFGNDPWLQPYTDETVEK
                     LAKKGIKRLVMVAPGFSADCLETLEELDGENREIFLHNGGENFSYIPALNASGPGLDV
                     IETLVRRELMGWVEDIHAMPESEAAIRAEATELDQRNLLP"
     gene            19832..20290
                     /locus_tag="PLQ11_00860"
     CDS             19832..20290
                     /locus_tag="PLQ11_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23480.1"
                     /translation="MRRGTVAGFVYGAAAFALGFLFGVIRVSLVTPRIGPLWAVLVEV
                     PVMLVLLWPLARRIASHWLVAVAFAPRLAMGTAALATLLTAEVALGMIGFNQGLDDVV
                     GRLASPEGLFGLAGQIAFASFPCWQMRRAMDCRAPSATLCAHRKQGSGLV"
     gene            20378..21391
                     /locus_tag="PLQ11_00865"
     CDS             20378..21391
                     /locus_tag="PLQ11_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRK23481.1"
                     /translation="MSGGGIFALMLFVFAIITLMAGIKQVPQGMNYTVERFRRFNRTL
                     SPGLGLVIPYIETIGHKVIMMEQVLDVPTQEVITKDNATVQVDGVVFFQVLDAASASY
                     EVANLHQALMNLTMTNIRTVMGSMDLDQLLSHRDEINSRLLRVVDAAASPWGLKVTRI
                     EIKDIVPPANLVEAMGRQMKAEREKRANILEAEGFKQAEVLRAEGHKQAEILAAEGRK
                     TAAFLDAEARERKAEAEAKATQVLSAAIVGGDTAAVNYLIAEKYVGALGQFATANNQK
                     VMIVPMDTAGLAGTLAGIAEITRNASPGGPPGGGQAAQRSQGFGSVPPHGGKPASPFG
                     KVE"
     gene            21398..21838
                     /locus_tag="PLQ11_00870"
     CDS             21398..21838
                     /locus_tag="PLQ11_00870"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HRK23482.1"
                     /translation="MMGSLGLWGWLALGFGLIAVEILLIPGGFPLWIGLAALAMGGIT
                     ALVTMGWEAELILFGLLSVVASVIAWKLHYQRSRADAADGLHDRVEQLIGRTFTLEEA
                     ISGGFGRVRIDDTSWRVSGADQPEGAKVKVVGVDGGTLKVEKAI"
     gene            complement(22018..22995)
                     /locus_tag="PLQ11_00875"
     CDS             complement(22018..22995)
                     /locus_tag="PLQ11_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HRK23483.1"
                     /translation="MSAAAPTIASALRTLDRETKGLAALSRALNDGLGAPFAQAVTRI
                     ATTRGRTIVTGMGKSGLVGRKIAATLASTGTPAHYVHPGEASHGDLGMITPDDVVLAL
                     SWSGETAELADIISYSRRFGVGLIALTSASDSTLAREADIALVLPKATEACPNGLAPT
                     TSTTMQMALGDALAVALLEARGFTPEEFRQFHPGGKLGARLTYVRQVMHVGAAVPIVA
                     AGTLMAEAILVMTARGFGCVGVTDTTGALIGIVTDGDLRRHMRPDLTTLPVDQVMSRA
                     PRTISGDDLAVEALEMLNRNRITAVLVVDGAGRPEGILHLHDLLRLGVA"
     gene            23137..24750
                     /locus_tag="PLQ11_00880"
     CDS             23137..24750
                     /locus_tag="PLQ11_00880"
                     /inference="COORDINATES: protein motif:HMM:NF021589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRK23484.1"
                     /translation="MTVAVARSLLPALLALTTALIGGPALAQVRGTVGQSTDRLYDPG
                     NADPGRMRSGTTGFDADPGLPARRSGNATPANPAELAGNSVVNSNTRVAVRKRTRSAA
                     TLTRPPNRAVIAPARPAQPVAGIAPPLAAPRAMPDIVAPGLTEPMVPRKKKKKEEDPY
                     APLGLRLGGLTVHAAVDLQAGYDSNPLRSGSGSAVKGSKFYQVAPEVSVVSDWSRHQL
                     QFDLKGAYLLFQDVNDADRPTLDAKLTFRGDISRDTTLNLELKEKVDTQRPGSLDLTS
                     AVKGRPAFYTHSGSIGVTQKFGYASITAAANVDRATYDQGVTYAGLSYDQSDRNYTTY
                     GARLRGAYEITPGIVPYVEAMVDSRQYDREFNASGYRRSSDGVIGRVGTTFEVTQQLT
                     GDVSAGYGMRRYDDSRLDHLSGLLIDASLVWQMSPLTKMTLKGTSEFVETATVGSSGA
                     VNRKVSVDLAHDLLRNVTVTGSLGYSQTLYQGIGLMEQTINAGLKFDYKIDRTFVVRG
                     SYAYERGLSDNAGQSYLAHVFLLGLRIQR"
     gene            24978..27290
                     /locus_tag="PLQ11_00885"
     CDS             24978..27290
                     /locus_tag="PLQ11_00885"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRK23485.1"
                     /translation="MPHQHTLADAGITLVENAAEESFDKVKVLVSSLFSSPASLASGA
                     VAALATGAICLYASGEILFVYWLIAALAINLYRLAFTRFFNTAVGDITTIAMLRYYER
                     HFVVGAMLQSLLMGTAAVIAILMTHSDAAQIASVTSAIAFSSGFVARNAARPRFVAAQ
                     LLVTVGPLTVAFFMSSMPDYNLIGLFAILFIVSNVVQTRSVHRNLVALSVATAREKEL
                     TQAVREKNRMLDIALAHMSQGLALFDEAGRLTVSNQRFLDFLSVSDAGNTHALPANQL
                     ASLLVVRGVLDRVSARALHKAWTDMQQNGGRPRFDLSLKSGHILDVILTPVEGGGFLM
                     TSEDVTAARKAQARIEHLARFDDLTGLLNRHEANVRLSQWCDRPNSFAVAYLDICQFK
                     EINGRFGHEAGDLTLQAVGRRLQSFVRDDGFVGRLGGDEFIVVLDVTDSAHAMRLLSR
                     LSQSLERPIMIEGRSLKAPVKIGFSMFPDHGTTALQLQRAADLALYRAKEKSIGPMAY
                     LTEMGQAAARLRDMEEDLRKALVKGGLELYYQPIIDLNTKTIVSFEALMRWKHPERGF
                     IPPSEFIPVAESTNLIVELGEWAIHEAARSIATWPRHVSVAVNVSPIQFASGERLLEV
                     VRSALAESGIPASQLTLEVTESVLIDDSGSVRDTMIKLRDMGVKCALDDFGTGYSSLS
                     YLASFPFYCVKIDRCFVKDIEASRASRATVEAVCHLARSLGIQTVAEGIEEAAQGDVL
                     AQIGVNRGQGYHYGRPAPWSEASARLSQAA"
     gene            27533..28438
                     /locus_tag="PLQ11_00890"
     CDS             27533..28438
                     /locus_tag="PLQ11_00890"
                     /inference="COORDINATES: protein motif:HMM:NF006452.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HRK23486.1"
                     /translation="MSVAASIAFKQSVSSDVLDAIYGWAGPLPQVELEFEAELKLLWL
                     TIRPEPKPIFTLQLLDSVVAVQKAIGHLWADLRPHDRPVRFLAFRGRGGVFTLGGDLD
                     FYLECIATGDRSRLMDYAEASVQGAIGNASGCYGSVITLSTIHAKALGGGIDAPRSCH
                     IMIAERQASFCYPEIRFNHFPITAVPVLSRKLGPMAAEALLLSGDEISAEEFRARGGL
                     EAVVEANQGEDWIRNYCQTNLESAAARLAIYGMTYEANRHYEQDLRDSAARWVQAMLN
                     MRPTEISKLQRISQMQEKLLARMRS"
     gene            complement(28567..29466)
                     /locus_tag="PLQ11_00895"
     CDS             complement(28567..29466)
                     /locus_tag="PLQ11_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HRK23487.1"
                     /translation="MTMTAPKNSLFRPVLLASALLAAGTATGADLEKSKKAMPPVAPA
                     AAPAWDMTFGARIVSDYNFRGISQTNRKPGVQGYAELTYQWLYAGIAGYSVDLPTKPL
                     AEIDFTYGIRPKVGPFTFDIGMIHYYYPREKQYVDGFGAIWTPKNTDFYEIAGKALYS
                     WEDKVFIGANLFHSPNWLGTGATGTYYSGTLKVVPPLPIEGLAVSGELGRYALGKTSA
                     YLGGLDLKDYTYWNAGVSYTYKAATLDLRYHDTDLSKANCFLNTSDPRGIVSGSGQSK
                     WCGAAFIATLSLDLSASGLGLIK"
     gene            29737..30144
                     /locus_tag="PLQ11_00900"
     CDS             29737..30144
                     /locus_tag="PLQ11_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23488.1"
                     /translation="MAGSGRQADSLTGVWHGQYAYPLLLEPVQFTATLIESGRQISGS
                     IHETGRRPGQVAQQLKAMLEGAVGGATLRFVKTYLPASDQHQDVIYIGTINAERTEIE
                     GEWIIPGDWSGRFVMIRMRDPAQSVEQRQMIEA"
     gene            complement(30192..31055)
                     /locus_tag="PLQ11_00905"
     CDS             complement(30192..31055)
                     /locus_tag="PLQ11_00905"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HRK23489.1"
                     /translation="MKKIRKAVFPVAGLGTRFLPATKSVPKEMMTVVDRPVLQWVVDE
                     AREAGIEHLIFVTGRNKAVIEDHFDIAYELEATLKERGKLKELEQLQKDLPPAGMATF
                     TRQQAPLGLGHAVWCARDIVGDEPFAVMLPDMLSKGCMKQMVAAYERHGGNIIALEQV
                     PMDQTHQYGIVAVGAAHGRVHELTGMVEKPPKGTAPSNYIISGRYILQPEIFDILSAK
                     EKGAGGEIQLTDAMIKLMAGQKFFGLHYDGRTYDTGSKIGFLSAQIAYGLDRAELRDE
                     FLDEIRRIAGL"
     gene            31268..32257
                     /gene="galE"
                     /locus_tag="PLQ11_00910"
     CDS             31268..32257
                     /gene="galE"
                     /locus_tag="PLQ11_00910"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HRK23490.1"
                     /translation="MTVLVTGGAGYIGSHMVHALRDRGERVAVLDNLSTGFAWAVPPD
                     VAFFGGDVADQDLVKRIIRDQEVTAICHFAAKIVVPDSVADPLGYYAGNTMASRNLID
                     AAIKAGVNQFIFSSTAATYGEPEIMPVSEDTPQVPCSPYGRSKLMTEWMLEDSARAYD
                     FRYVALRYFNVAGADPFGRTGQSTPNATHLIKAASQAAIGLRKHLDLYGTDYPTPDGT
                     CVRDYIQVSDLADAHVLALNYLRSGGISTVFNCGYGKGFSVREVIDMVKKVSGVDFPV
                     NVQGRRAGDAAAIVAKADSIRQKLGWMPKHDDLEEIVRQALRWEQHLRSRNAA"
     gene            32288..33484
                     /locus_tag="PLQ11_00915"
     CDS             32288..33484
                     /locus_tag="PLQ11_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012753285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HRK23491.1"
                     /translation="MPSLTRALRSLVMAVVLLAGPAALPARADFKAFVAGLWPEAQAA
                     GVSRATFDAAFAGVTLDASVVAQTKRQSEFIRPIWDYVGSAVSTSRISRGRAMAEQWS
                     ETVAAVERRYGVDRRVFLGIWGMETSYGSFFGGHSVIRALASLAYVRYRDDFFRKELI
                     VALQILEEGHVEKADMKGSWAGAMGHTQFMPSSFMKHAVDATGDGHKDIWGSVPDALA
                     STANYLKQHGWQSGLPWGFEVILPEGLDFKQALGRKSFSGFAALGIKRADEEAMPSRG
                     EANLFLPAGIKGPKFLITDNFKVIKAYNSSDAYAMGVGLLGDTIYGGEGLTGRWPKAD
                     KRITKAEGVAIQKLLNQRGLYNGKYDGRFGEQSREAVTRYQLSVGMVPDGYATPALLA
                     RLKGSR"
     gene            33543..34874
                     /locus_tag="PLQ11_00920"
     CDS             33543..34874
                     /locus_tag="PLQ11_00920"
                     /inference="COORDINATES: protein motif:HMM:NF016218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH family hydrolase"
                     /protein_id="HRK23492.1"
                     /translation="MLTALLVLVVVWSGAIGEALAQGDGSSVYTTAPKGRNDRGPLDW
                     LFGRRGGSVTPVIPQAAPRADSKPRTYRPARRTTPSTLPTVPGDPAAPADTNAPSVAG
                     DPAAPAPAPPPALPPVSVVVAGDSLSVFLGQGLQELYAEKPHVSFVKRHRDASGLVRD
                     DFHDWPKAFRDLAAGARPDALVLMIGSNDRQQLRDDQGVHEPLTPRWKELYAKRIDDV
                     VAAIRGQGIPLIWVGLPVMRSEKYSVDLTALNDLYRARAQAAGIAFVEIWEAFAGEDG
                     HYTVNGPDVGGEIVRLRTSDGVHFTKAGARKLAFFVDQELAKLLGAIKPQPPLPQQPA
                     TPQVDPGDLPPPPQVMAPPGPERFPAPASPSFDAMLGVPMPEIAAAPTLVPRPAQGPV
                     MPLNAAPLTTGGQLLSAQTVNRLDEARKVFVQGVGAPPKPGRLDDFRPPEP"
BASE COUNT         6445 a        10511 c        11253 g         6735 t
ORIGIN      
        1 gccgatgccg aacggtgcgc ggccggtccc ctgggccagc acggtctcgg cacaggccag
       61 acagacagca gccagcacga gggccgccac ggcggagaag acggcaggcc ggcggatcat
      121 ttggcgcagg caaggcggat ggtggtggcg aactggtcgg tgaaaccgga tttcagattg
      181 gcgaacatgt cttcggaaag acgcgagaga tcggcactgc caaggctctt ctgcggcttt
      241 ttcatctcca gcttgcagga gcctggattg ccctcgatca tcacgggatt atcctcggca
      301 aacgaaaagg cgacgaagaa gctctcgtcg tcgacccgca gcgcgatgga actgtcggcg
      361 acgacgggcg tcttgagcgg cagcaggaag tgcagcacca gtgccttgcc gttgtgttcg
      421 agccggtagc ccggtggcgg ggcggcgaac tcaagctccg tccggccctg cttgagcgag
      481 gtgaaatact tgaactcgcc catcgattcg acattgagcc tggccagatc gtcgagttcc
      541 ctgcgggcga agctgccgtc cttctccttg ggaaatccca tggtggcata ggccgaatag
      601 gcttcatcga agatccatga atggcgcacg ccggtgacct tgccggcgtc catcaccagc
      661 gcgctgcggg cggtgaccat gacatgggga tgggccgcag ccggggccgc cgcgatcagg
      721 gctgcggcaa gggtccagcc gcacaggggt ccgcttcgtc tcgcgttcat gctgcgccct
      781 cttcctgcaa ggctttctcc tgaatctagt atctattccc gttcctgcaa gcggcaggtg
      841 catggcgatc gctcgtcaga cctgtggcgg gcggctcgtt cgccccttct catgaaatca
      901 ccgtctggcc ggcggagagg ccgggcccgc cgctcgcgtc ggcatagctc ccggtcagcg
      961 tataggcctg cgcgaagaac gaggcggctt cgtagtcctg cggctgcgcg tccgttcgaa
     1021 gtgtcgcatt tccaattccc gttgccgccg atcccggcaa cacaactcct ggcaggtatc
     1081 cacggttgta ggaatccatt gcgagaatgg cgagaaacag gtctttcgat ataaccattt
     1141 cgtcagctcc ttttcaaatt tcgtccaggc atgagacttg aaccgcctgc gtttccgcgc
     1201 aggaggcgtt cgaacgtcgt ttctttggcg tagtcgccga tccagttgat cttcccctcg
     1261 atcccggtcg ttatggggtc ggttgtcatt tcgatggtga cgcgtttgag ggcgtagcct
     1321 tttccgaagg tctcttcgaa gcggtcgatg acgagggggc cgaagtcgcc gtaggcaccg
     1381 ctgctgctgc cgggggaatc cggcacgaac cggatgtcgc gccccgtggc gtagaccacc
     1441 ttcgcgctcg ccgggtcggc aaggtcggtg aaggtcacta tcgtcggcac cagttccggc
     1501 gcgagttcta caatgccttc gagcttacgg cgaccgcgcc agatatcctc attgccccgc
     1561 tgcatgaaac cggcataggc ctgcacccag agagtgatca tcgtgtcact atcctcagcc
     1621 cttggcccgt gcgccaacag gccgatggca tgacggccag agccgaaatc gacgaacacc
     1681 gcttcgccgg tgatcgtgtg attgaaccgg ttagcgatcc cgctgagcgg catccatttg
     1741 atgtcagtcc ggttcacgtt gataacgctt gaggcgcttc gaaccccatc tggcgtcgtg
     1801 atttccacgg tcaaccggaa gcggtgatca acctcggtcg cccgcatata gacccagccg
     1861 acgccaatcc atcccgccag cgcgaggatt ggcaggccga tcagcaaccc aaggctccag
     1921 aacaaggctt tcatgccgcg cgcccccaat ccggcgctgc cacggagcgg ggccggagag
     1981 gggcgagcct tccggcaaac gccgccggcg tgtcccatcc gctgtccgga ccgcccctaa
     2041 acgcacgcct gttccgccag cgaccggggg cctgcatgcc ccccggtctc gtcctgccgc
     2101 cctgcccatg cgcaatcatg atcgccccgc aatcgcattt ataaaatcag agtccggggt
     2161 cgacctggcg cccggatatg cctgatccta tactgatttg gccctttcgc gcaggggatg
     2221 gcagcgcctg cggagcgcgc cactcggtcg cggtcgcttg acacggcgtt ttaggtaagt
     2281 attattgaca tatacgaaag acggaaacac gggaagaatg cgccgaagcg attcggcccg
     2341 tgaaaggccc gacaggacag ggccgcgtca gggaggatgc gatggaattg cctctcgcct
     2401 tgcggcaggt cagccttgcc gacaaatacg atctgtcgaa aaacgaggtc tttctttccg
     2461 gcacgcaggc catcgtgcgc atgctgctga tgcagcgcga gcgcgaccgg cgggccgggc
     2521 tgaacacggc gggattcgtc tccggctatc gcggctcgcc gctcggcgga ctcgaccagc
     2581 aactcatccg tgctcggcgt gagatcgctg cggcgccgat ccggttcgag ccggggctca
     2641 acgaggatct tgccgccact gcctgctggg gcacccagca ggcggagatg cgcggcgagg
     2701 gcagatacga tggcgtcttc gcgctgtggt atggcaaggg gccgggcgtc gaccgaaccg
     2761 gtgacgtgtt ccgccatgcc aacatggccg gtacgtcgcg tcatggcggg gtgatcgcgc
     2821 tcgccggcga tgaccacacc gccgaaagct ccaccaacgc gcatcagacg gagttcaact
     2881 tcgtcgatac gatgatcccc atcctcaacc cggccggggt gcaggaaatc ctcgactatg
     2941 gccagcacgc ctatgcgatg agccggtttg ccggggtatg ggtggcgctg aaggcggtca
     3001 aggacaatgt cgaatcaacc gcttccgtcg acggttcgct ggagcgggtg tcgatcctga
     3061 cgccgggcaa ttatccgatg ccggatggcg ggctgaacat ccggccgcgc gacaacttcc
     3121 tcgaacagga gcgacgcctg cacgagttca agcgcgaggc ggcgctggcc tatgtgcgcg
     3181 ccaacggcct caaccagatg gtcttttccg ggggagccaa gccgaggatc ggcatcatca
     3241 ccgtcggcaa gagctatctc gatacgcggg cggcgctggc cagtctcggc atcgatgcag
     3301 gccgggcgca gacactgggt atccggctgt tcaagatcgc ctgtccgtgg ccgatcgacc
     3361 cggaggaact caaggatttc gcacgcgggc tcgataccat catggtcgtc gaggaaaagc
     3421 gttcgctgat cgaggtgcag gtgcgtgagg agctgtatgg ctcggccaat cagccggtcg
     3481 tcgtcggcaa acgcgatgag ggcgggcaat ggctgtttcc ggtcaccgcc gcgcttgatc
     3541 ccaacgatat tgccatcgcc atcggcgagc ggctgatcgc acggcaaccc aacgaagaga
     3601 tcagggcccg gatcgccggg ctcaggcagg cgcagacagg gctggcacgg atcgcggaag
     3661 tggcggcgcg tgtgccgtat ttctgttcgg gatgtccgca caattcgtcc acggtggtgc
     3721 cggaaggcgc gcgcgcctat gccggcatcg gctgtcacta catggtgcag tggatggatc
     3781 gggaaacgga aggcttcacc cagatgggcg gggaaggcgc caactggatc ggcgaatcaa
     3841 ccttctcgac gcgcaaccac gtattccaga acctcgggga cggcacctac aaccattccg
     3901 gttcgctggc gatccgctgg gcgatcgcgg caaagaccaa catcacctac aagatcctgt
     3961 tcaacgatgc cgtggcgatg accggggggc agacccatga gggcgcgctg acgccctggc
     4021 ggatcgctca gcaggtgcgt gccgagggtg tcgaacgcat cgcgctggtt tctgatgatc
     4081 cttacaaata cggcgccgag gccaaatggc cgccgggggt gagtttccat catcgctcgg
     4141 agctgatgcc ggtgcagaag gagctggccg caatccccgg tgtcactgtc atgatctatg
     4201 accagacctg tgccgccgaa aagcgccgcc ggcgcaagcg cgggctctat cctgatccag
     4261 ataagcgcgt catcatcaat gaactggtct gcgagggttg cggcgattgc ggcaagaaat
     4321 ccaattgcgt ttcggtgcag ccgctggaga ccgaatacgg ccgcaagcgg cgcatcgacc
     4381 agtccaattg caacaaggac ttctcctgtc ttgaaggctt ctgtccgtcg ttcgtcacca
     4441 ttgagggtgc gcggctgaag aaagcggctt cgattgccgg aatggcggca atcccctccg
     4501 tgccggaacc ggaattccgt gatctcgacc atgatccctg gtcctgcatc gttaccggcg
     4561 tcggcggcac cggtgtggtg acggtggggg caatcctcgg catggcggcg catctggagg
     4621 ggcgcggctg cggcatgatc gacatggcgg ggctggcaca gaagggcggt gcggtgtttt
     4681 cgcatatccg tctcgcccgg agcccggaag acattcatgc catccgtgtc gaggcgggct
     4741 cagccgacct cattctaggc tgcgatcttg tggtgtccgg atcgcgcaag gtgctggcag
     4801 cgatgcggca gggggaaacg gtggcggtgg tcaataccgc cgaggttgcc tcgggcgaat
     4861 ttacccgcaa gcccgactac caattgccgg ccgaacggct gaaacgctcc atccgtgaag
     4921 cggcaggaga ggggcgggcc gatttcgtcg atgccaccgc catggccgcg cgcctgttcg
     4981 gcaatacgct tgcggcgaac atgttcatgc tgggctatgc atttcagcgt ggcgccgtgc
     5041 cgctcgtttc tgcggcgatc gaacaggcga tccggctcaa ccaggaatcg gtcgagatga
     5101 atctcgccgc gttccgctgg gggcgggtgg cgctgcatga catggcctcg attcgcaggc
     5161 tgatcgacga gaccattcct ccgacgcggc aggagacctt cagccagggg ctcgacggat
     5221 tgatcgagcg gcgcatggaa ttcctgacca actatcagaa tgcaggctat gcgctggcct
     5281 atcgcaggcg ggtcgaggtg atccgcgcag cggaagaggc gcgtatgccc ggctccaccc
     5341 gcctgaccga ggcggtggcg cgctcgtatg cgaaactgtt gtcctataag gacgaatacg
     5401 aggtggcgcg gctctatacc tccggctctt tcctcaagca ggtgaacgcg atctttgaag
     5461 gtgacatgcg cttcaccttc cacatggcgc cgcccttgct caaccgcagg catccatcga
     5521 cgggcgagcc gatgaaatcg agtttcgggc catggatgat gcgggcgctc aaggtgctgg
     5581 cgcggctgaa gggcctgcgc ggcacggcct tcgatccgtt cgggcggaca gccgaacggc
     5641 ggaccgagcg ccagctcatc gccgattacg aggtgctgct cgacgagttc tcccgttcgc
     5701 tgggccccgg caaccacgct gtcgctgtcg aactggccag cctgccggag cagatccgcg
     5761 gcttcgggca tgtcaaggac aggcatatcg aggcggcgcg tcgcgagcat gaaaggctga
     5821 tgcagcgcta tcgtgccggc ggcgacgggc ctccgctgcg cgcggcggcg gaataacgat
     5881 gtcgccggcc gatctggagg atttcttcgc gcccttcgcc aaggtcaatg tccggcggat
     5941 gttttccggc catgggatct atgtcggcac agcctgtttt gccatcagcg ccttcggaac
     6001 gatctggctg aaagccgatg cggacacgga ggccgcgttc gcggcggcgg ggtgtcgtcc
     6061 attctcgatg acgacaccgg atggttcaac cagaacgata cgcagtttct ggagcctgcc
     6121 cgaaccggca ttggatgatg ccgaggccct caagcgctgg tgtggcccgg cattgcgggc
     6181 tgcgcaggcg acggccctag agcatgctgc gaaaaagtgg aaacgggttt tcgcgaaaag
     6241 catgctctaa actactgtaa tcgatcacgt tatctctcgt tttgatgagt ccatcaaaac
     6301 gggagcgtga tctagagcat tttcaagcga agtggattct ggttcacgtt aagaaaatgc
     6361 gaaaaaacaa aaagatagag cactttctgt gaattggaat tcactgaaaa tgctctagcg
     6421 aaggcggcca agctccgacg tgcaagggcg caggccgacc gcgtctgacc cctcaggccg
     6481 aattgccggt gacaggctcg gagcaatcct tatattagaa aaaattcatt aaccggaggc
     6541 catcatgcgc cgcactcttc agatcgtctt gctggctctt tcgacgagcg ccctggcgca
     6601 atcccccggg ggacacacgg ggcatggcgg tatggccgcg cagggctttg tgttgccgga
     6661 cgatccgcgc accgccgtcg agattccgcc cgatgtgcgc gcgttcctgc gctacgaaat
     6721 gcgcgggcat ctcgaacagc tcgccacgct catggcgcgg atcggcgagg gcaacttcaa
     6781 gcaagcggcg gcatttgccc gcagcggcct tggagtcatg ggaaatcatc cgccgggcgc
     6841 cccgaatccg gctcagttcg tgccgcccga attccgcatg atgggccagg cgatgcatcg
     6901 cgccgccaat gcggtggccg aagcgggcga agctgccgtt acgccgccca gcgcagccga
     6961 ttggaagaca atggcagcgg cggcggcaaa tctcagcgcg gcctgcgccg gctgccatgg
     7021 ttcgttccgg ctgcgataag accttcgagg gccggccatg cccctttttg cgtgggtgac
     7081 aaggcgggcc gccttcagga taatgccgcg gtgatcgcct gcgatgccct tgcccgactt
     7141 gcccattggt ccggagacct cactccggat gagcggagcc gcgcggcccg cggtgttaca
     7201 gagaagacgt ttgcaaaggg cacctatatc tgccacctcg gtgaccggct cgattgctgg
     7261 gttggtgtct ccgaggggct gctcaaactc tcgaccgtaa cccgaaacgg caaggcaatg
     7321 accttcgccg gcatcgccga gggtggctgg ttcggcgaag gatccctact caagaacgag
     7381 ccgaggcgct atgacgtggt ggccttgcgt gactgccggc tggcgttgct cgaccgggcg
     7441 actttcctgt ggctgttcga acattcggtc gggttcaacc gcttccttgt ccggcacttc
     7501 aacgagcggc tgagccagtt catggcacag gtcgaattcg accgaacgct caatgcaacg
     7561 gaacggctgg cgcgcaccat cgtctggctg ttcaatccag tgttctatcc gaaagcgcgc
     7621 agtcatatcc gcatttcgca agaagagatc ggactgctgt gcggcttgtc gcgccagagc
     7681 gccaatcagt ccctgcaggc gctggaagca gccggactga ttgccatgga ggcaggcggc
     7741 atgaccgtcc gggacctggc aaggctgcag cggttcgaaa cctgaaccaa tccggtagtg
     7801 taagagccgc aagccctggt gtctgtcaag cccgggcttg catcaagcgc gcgttgctgc
     7861 aagtctgccg gcaaatcaga cgggagcgcc ggcggcgcga cccgttgcat tctgttcagg
     7921 gaggctcaac ttgtctgaca cgaccgctgc gtcgcgcgac acgtttccga aatggctggc
     7981 acatcatgcc agggttcgcg gcagccgacc ggcaatgcgc ttcaaggaag ccggcatctg
     8041 gcagcgctgg acctgggcag agatcttcga catcgttcgc gattatgcgg tcgggctgac
     8101 tgatcttggt ctcaagcgtg gtgacaagat cgccatcgtc gggaccaatc ggcccaagct
     8161 ctactggtcg atcatggcgg cgcagtgcct cggggcggtg ccggtcccgg tctatgccga
     8221 tgccgtggcc gacgagatgg cctatgtgct cgatcatgcc gaggtggtga tggcggcagt
     8281 cgaggatcag gaacaggtcg acaagctgat ttcggtgcgc gatcgcgtgc cgcgcctgag
     8341 acacatcctt tatgatgaac cgcgcgggct gagggattac gaccacagcc acctgcatcc
     8401 gatttccgcc atcatcgcgc gcggcaaagt gatgcagcag gaggggccgg acgtgcgcgg
     8461 cgccatcgac cggtcgattg ctgccggcaa ggccgacgac ctgtccatca tgctctacac
     8521 atccgggacc accggacgat ccaagggcgt gatgctgtcg gcgggcgggt cggtgaatgc
     8581 ctgttccgat acggtcaagt tcgacaagct cagcgatacc gacgaggtga tcgcctatct
     8641 gccgctcgcc tgggtgggcg accattatct caactacggg caggcgctgg tcgccggctt
     8701 ctgcgtcaat tgtccggaaa gtcccgagac tgcggtcgat gaccggcgcg agatcggggc
     8761 aaccttcttc ttcgccccgc cgcgcgtgtt cgaaggcatg ctgacacgca tcatgatccg
     8821 catggaggac gccggaccgc tcaagcgccg gatgttccat cacttcatcg gcgtggccaa
     8881 gcaatggggc gagaagatcc tcaacggcga gacagtgccg ctgggcgccc gattgcatta
     8941 tcagctcggc gaatggctgg tctatggccc gctcaagaac gtcatgggat tttccaagat
     9001 gcgtgtcggc tataccgccg gcgaggcgat cgggccggag ttgttctcgt tctaccggtc
     9061 gatcgggctt aacctgaagc agctctacgg acagaccgag gctttcctct atgtcacctg
     9121 ccagccggac gggcagatca gggccgatac cgtcgggccg cccgcgccga atgtcgatat
     9181 caagatctcc gaggatggcg aactgctcta caagtcaccc ggcatgttcc agggttattt
     9241 caaggagccg gaaaagactg ccgagacgat gacgcctgac ggctatgtcc gcaccggcga
     9301 ttccggcttt tacgacaaga ccgggcatat ccgcatcatc gaccgggcca aggatgtcgg
     9361 caagctcgct tcaggcgcct tgttcgcgcc gaaatacatc gagaacaagc tcaagttctt
     9421 tcccaatatc aaggaagcgg tcgccttcgg tcagggcaag gatttcgtca ccgtgttcgt
     9481 caacatcgac atgacggcga tgggcaactg ggccgaacgg cataatgtgg cctatgcctc
     9541 gtatcaggaa ctcgccaacc atccggatgt ctacaagatc attgccgggc acatggacga
     9601 gatcaacaag tcgctggccg cagaaccgat gatggcgcgt gcgcagatcg cccgcttcct
     9661 gatcctgccc aaggaactcg acgccgacga tggcgaactg acccgcacgc agaaggttcg
     9721 ccgcggcttc atcgccgaac gatacgagcc gctggtcacg gcgctctacg acgggtcgaa
     9781 ggaggcggac atttcggttc aggtgacgtt cgaggatggc cgcaagggat cgatttcggg
     9841 tcgtgtgcgg gtcgccgacc tcaactgtta tccggtgatc gaggagaagg cggcatgagc
     9901 ccgcaggctg ggatcgagcg cggcgatgtg ctgctggcgg tggacaacgt gtccttgcgc
     9961 ttcggcgggg tcaaggccat caccgatgtg tcgttcgata tccgcaaggg cgagatccgc
    10021 gccatcatcg ggccaaacgg ggccggcaag acgtcgatgc tcaatgtcat caacggcttc
    10081 taccatcccc aggaaggggc gatcacctac aagggtgtca agcgcgagaa gatgcgcccc
    10141 tatgtggcgg caagccaggg tatcgcccgc accttccaga atgtggcgct gttcaagggc
    10201 atgacgacgc tggacaacat catggtcggc cgcacgctga agatgcgcac gccgttcttc
    10261 tggcaggtgt ggcggcatgg tccggcgatg cgcgaggagg tcgagcaccg caagttcgtc
    10321 gaggagatca tcgactttct tgaaatcgag cacatccgca aggtgccggt gggcaagctg
    10381 ccctacggct tgcagaagcg ggttgaactg gggcgcgcgc tggcgatgga accggaattg
    10441 ctgctgctgg acgagccgat ggccggcatg aacctcgagg agaaggagga catgtgccgc
    10501 ttcattctcg atgtctccag ccagtatggc acgacgattg cgctgatcga gcatgacatg
    10561 ggggtggtga tggacctcag cgatcgcgtg gtcgtgctcg aatacgggcg caagattgcc
    10621 gacggcacgc cggccgaggt caaggccgat ccgaccgtca tcgccgccta tctcggcgtc
    10681 gggcactgag ggggcgggca tgctctacaa cgttttcatc gatcccttcc tgcagatggt
    10741 ccaggcgcct gacctgctgg ttcagacgct gtgggagggg ctggtcgccg gcgttctcta
    10801 tgcgctgatc gcgctcggct tcgtgctgat cttcaaggct tcgggcgtgt tcaatttcgc
    10861 gcaaggcatc atggtggtgt ttgccgcgct gacgctggtg ggcctctatg agaagggggt
    10921 tccggcgctg ctggcgctga tcctgaccat cggcgtgatg tatgttctgg cctggagcac
    10981 ggaacggttc gtgctgcggc cgctggtgaa ccagcctgat atcatcctgt tcatggcaac
    11041 cttcgggctg acctatgtgc tgatcggcct cggcgagttc atcttcggtg gcaacaccaa
    11101 gacgatgatc accgagcagc tcttcctgcc caagggcgcg atcgacgtgc agatgctcgg
    11161 cgggcgggtg actttccaga agctcgacat cgccgcagca gtcatcgccg gggcgatggt
    11221 ggccgggctg gcggcgttct tccagtatac ccgcatcggc cgcgcgctcc gtgcggtggc
    11281 cgacagccac aaggcggcgc tgtcggtggg catctcgctc aaccagatct gggtcatcgt
    11341 ctggttcact gccggcatcg tggcgctggt caccggcatc atgtggggcg ctcgctcgga
    11401 cgtgtcgttc ggcctgcaga tcatcgcgct gaaggcgctg ccggtgctga tccttggcgg
    11461 cttcacctcg gtgccggggg cgattgtcgg cgggctgatc atcggcatcg gcgaaaagct
    11521 gggcgagttc tactggggcc cgctggtcgg cggcggcatc gaaagctggc tcgccttcct
    11581 gatcgcgctg gccttcctgc tgttcaggcc gcagggcctg ttcggcgaca agatcatcga
    11641 gcggatttga gggggacagg cccgtgttct atcgtgaagc aggacaattc aagtcgactt
    11701 acgaatccga catggcggtg ttccccatcc gccaggatct gatcggcgtg ctggccatca
    11761 tcgcggtagc ctatgtgctg atcccgatga ccggaaaatt cttcatcggc acggtgatga
    11821 tcccgttcct ggtgttcgtg atggcatcga tcgggctcaa tatcctgacc ggctataccg
    11881 gcctgctgtc gctgggcacc ggcgcgttca tgggggtggg tgcctatgcc tgctacaagc
    11941 tgacgacgat ctttccgggg gtcaacatcc tgatctggat cgtggtgtcg ggcttcttct
    12001 cggcggcgat cggggtcctg ttcggcctgc ccagcctgcg catcaagggg ttttatctcg
    12061 ccgtggcgac gctggcggcg cagtttttcc tgcaatggtg ctttgtgcgc attccctggc
    12121 tgttcaatta caacgcctcg ggcgccattg aagtgccggc gcgcatggtg ctggacattc
    12181 cggtgacagg gccgaacgcc acagaactca cccgttatta tgtgctgctg accatggtgg
    12241 tgttcttcac ctggattgcc tccaatctgg tgcgcggccg ctatggccgg cagtggatgg
    12301 cgatccgcga catggacatt gccgccgaac tgatgggcat ccgaccgctg caggccaagc
    12361 tgttgtcctt cgccgtctcc tcgttctact gcggcgtcgc gggggcgatg atgatgttcc
    12421 tgtggtatgg cggcggcgag gcgtccgatg cgttcaacat caacttgagc ttcaatatcc
    12481 tgttcatggt gatcatcggc ggccttggca gcatgatcgg ttgctttttc ggcgcggcga
    12541 tgctgcatct ggtgccgatc ttcctcaagt tcggtctgcc ggcgatcggt tttccgatcg
    12601 gcggagccac cgccgagcat ctgacgcatc tgatcatcgg cgttctgatc atcttcttcc
    12661 tcatcgtcga accgcatggc cttgcccgcc tctgggcaac cggcaagcag aagctgagga
    12721 tgtggccatt cccgtactga tcccgtccgg cggtttggcc gggatggatc ggaacgggtc
    12781 gtcgggcacc ggatgtcggc gcccatcgtg tcttgacccg gaggcctccg ccatccgggg
    12841 tgagtgaaaa gggaggatac cgcatgtcac tcaagaaact ggcgctcggc cttgccgccg
    12901 ccaccgccat caccaccttc gccggcgctg cgcaggcgca ggacagcatc tatgtgccgc
    12961 tgctgaccta tcgcaccggt ccgttcgcct cgtcgggcgt gccgatcgcc gatggcatgc
    13021 acgactatct gcgcatgctc aacgagcgcg acggcggcat cggcggcgtc aggctcacga
    13081 tcgaggagtg cgagaccggc tacgacacca agaaaggcgt cgaatgctac gaggccacca
    13141 agggcaagaa gccggcgatg tacaacccgt actccaccgg catcacgctg cagctgatcc
    13201 ccaaggcggg ggtcgacaag gttccggtgc tgtcgatggc ctatggcctg tcggcggcgg
    13261 cggtgggcaa caccttcccg tggatcttca acccgccggt gacctactgg gacggcgctt
    13321 cggtgttcat caagcacatc atcgagaagg aaggcggggc tgacaagctg aagggcaaga
    13381 agatcggcct gctgcatctc gacgcgccgt tcggcaagga gccgatcccg ctgctcgaga
    13441 acctcgccaa ggaatacggc ttcgaactga agctctatcc cgtgccggcc gacaagatgc
    13501 aggaccagtc ctcgcagtgg ctggcgatcc gccgcgaccg caccgactgg atctacaacc
    13561 agggctgggg cgcgatgaac ccgacggccg tcaaggaagc ggtgaagaac ggcttcccga
    13621 tggacaagat ggtcggcgtc tggtgggccg gcggtgatga cgatgcgcgt gccggcgagg
    13681 cgggcggcaa gggctacaag agcctgaact tcaacggcgt tggcaccgaa ttcggcgtga
    13741 tgaaggacat cctcaagcac gtcgtcgaca agggcctgtc gaaatatccc aaggacaagg
    13801 tgggcgacaa tctctacagc cgcggcgtct ataacgccat gctgatcgct gaaggcatcc
    13861 gcacggccca ggccgtgacc ggcaagaagg cggtggtcgg cgaggatgtg cgccgtggcc
    13921 ttgaaagcct caacatcacc gaggcgcggc tgaaggagat gggcatggaa ggcttcgccg
    13981 caccggtgaa gattgcctgc aatgatcaca atggccacaa caaggtctat gtcgcccagt
    14041 gggacggcac aaagtggacc aaggcgtcgg actggatgag cccgatgacc gacaaggtgc
    14101 gcccgctgct cgaggcggcc gccaaggact atgccgagaa gaacaactgg ccggcgcgca
    14161 ccgaagcctg cgacgccaag tgagtagcaa gtgacataaa tggggagggg gctccggccc
    14221 cctccctcaa tcccgatcgg gacgcattct atcatcagcc gcaaggaccc agcgatgagc
    14281 gccgccgcag cagcacccgc aatcgagccc gcagccgaca ccatcctgtc ggtcaacaac
    14341 atcgaggtga tctacgatca cgtcattctg gtgctcaagg gcgtgtcgct gaccgtgccg
    14401 aggggtggca ttgtggcatt gcttggtgcc aatggcgccg gcaagacgac gacgctcaag
    14461 gcggtctcga acctgctcca ggccgagcgc ggcgacgtta ccaagggatc gatcgttttt
    14521 gagggcgaac ggatcgacca gttgaccccc aacgagctgg tcaagcgtgg ttgcatccag
    14581 gtgatggaag gacgccactg cttcggccat ctcaccatcg aggaaaacct gctgaccggg
    14641 gcgtttacgc gccgcgatgg agcccgcgcc atcaaggatg atcttgagaa gatctacaat
    14701 tattttccgc gcctgaggca gcgcaaggcg tcgatggccg gttacacttc gggcggcgag
    14761 cagcagatgt gcgccattgg ccgggcgatg atgagccggc cgaagatgat cctgctcgac
    14821 gaaccgtcca tgggactggc gccgcaggtg gtcgaagaga ttttcgagat cgtcaaggat
    14881 ctcaacgcca aggaaaatgt ctccttcctg ctggccgagc agaacaccaa tcaggcgctg
    14941 aaatacgcca cctatggcta tattctcgaa accggccgcg tggtgatgga cggcgaggcc
    15001 aaggcgctgc gcgagaacga ggacgtgaag gagttctacc tcggcgtcgg cgagggatcg
    15061 cgcaagtctt tccgcgacgt caagagctac aagcgccgca agcgctggct cgcgtaatcc
    15121 tcaaagcgcc gtcatggtga gcctgccgaa ccgtgctgcg ccgatctcct ggcatcatcc
    15181 tgcgacatgc tcaggatgat aggcccccat tgctgagaaa accgtccatg gcccagtttc
    15241 ttgacaagct cgaaacccgc gatcccgaaa agcgcgagaa ggcgcttttc cgtgatctgc
    15301 gcgaccttct tgtctacgcg gctcccaggg cgccgacgct gagaaagcag ctgcggggca
    15361 ttgatgtcgg caagctgaag gggcgcgacg atcttgcgaa ggtgccggtg atccgcaaga
    15421 cggatctgaa ggcgatgcag caggcgctgc cgcccttcgc cggattggcg gcggcccggc
    15481 ggggcgatct tctgcgcatc ctggtttctc ccgggccgat tttcgagccg gaagggcggg
    15541 gcatcgattg gtggggtgct gcgcgcgctt ttcatgcggc cggattccgc aagggcgatc
    15601 tcgtccacaa ctgcttttcc tatcatctga ctccgggcgg gcacatcatg gaatcgggcg
    15661 cacgcgcgct gggctgcccg gtcattcctg ccggggtggg caataccgaa cagcagcttg
    15721 aagccatcgc cgggctcaag cccgatggtt atgcgggcac tccggactat ttgaaaatgc
    15781 tgctcgacaa gggcagtgaa gccggaatcg acatgggctc gctgcgtaaa gcgctggttt
    15841 ccggcgcggc ctttccgccg tcgctgcaaa aagagatcgc ggggcgcggc atcgaagcct
    15901 atcaggccta tgcgacagcc gaactcggta tcatcgccta tgagacgccg gcgcgggccg
    15961 gattggtggt caatgaggac ctcatcgtcg agatcgtgcg gccgggcacc ggtgagccgg
    16021 ttgcggaggg cgagatcggc gaggtggtgg tgacgcgcct caacggcgat tatccgctgc
    16081 tgcgcttcgg caccggcgac atgtcgaagg ttctggcggg tgcgagccca tgcggacgca
    16141 cgaacatgcg gctggccggc tggctcgggc gtgccgacca gaccaccaag gtcaagggaa
    16201 tgttcgtgca tcctggtcag gtcaatgaga tcggcaaacg gcatccagag cttggacggc
    16261 tgcggctggt cgtcacccgc gccaacgagc aggatgtgat gacgctgaag gccgagacgt
    16321 catccaccga catcggtctt gcagcccgcg ttgcggagac gctgaacgcg gtgagcaagc
    16381 tcaaggggca ggtcgacctc gttgccgtcg gcggcctgcc gcaggacgga aaggtgatcg
    16441 cagacgatcg gcccatctga cctcatacca tcggtcctta agactgacca atcggtcaat
    16501 cttgaggacc aagcggtatt acttgaccgg gaagcgattg ccggcgttat cgaggatgaa
    16561 gtaggccttg ccgttgcgca ccacgggata gcagttagtg cccccgagat cgtagaccag
    16621 gcagatctga cgattggtgg cccgccattt gccggtgaac agacctgaga aattgaacga
    16681 ccggtccttg ccgaaattgg cgtatccgcc acccatgaat acctgcttgc cggcgaccag
    16741 gttagcgatc tcggcgttca gcagacgctg atcggcagaa tgagcagccg tagcggcaag
    16801 accggccagc aacagaccca gggcaatttt cttcatgtca tgaccctccg accggggcgg
    16861 acttggtgcc cgcagcccgt agcttgggat ggcgcaaatc attgtgtcga tcactgtggc
    16921 aatccgccgc gatttgggca gtaatcggcc atgggagccg ggcaaccggc caatgcctgc
    16981 catattgtcg gaggctgtcg tttggctggc gctaggcaat gatcagcgcg cggtgatgcg
    17041 tccctcgact ttgctttcga cggttccaag cttgcggata taggccatca ccacattggc
    17101 catcagcgtt ccgtcggtct tgccgatgag ggtgcggccg cgcccaagcg cggagtatcc
    17161 gtcgccgccc ccatacatga agtcgttgga ggcgaccttg tatcgtttgt ccgggtccag
    17221 tggtttgccg tcaacctcga tcgaggcaat gcggttgccg gccggtgcct tgggatcgta
    17281 aaccagctta atgcccgaaa tctggggaaa ccgaccggcg cggttgtcca cctgcgacag
    17341 cccgttctcc agcgcttcct tcaggtcctt gccggcaacc tccaccatca ccgtagaatt
    17401 gccgaacggc agttccgtca gcacgtcgcg tcgtgtcagc gtggctcccg ccgggtattg
    17461 cttgttggca cggatgccac cgccgttgat caccgccacc tcggcgcctg tcgaagcacg
    17521 gatggcatcg gccatgaggt tgccaatcgc tgcttcctga gatcggacca cggcgctgcg
    17581 gctgtcgagg tcaacggcgg tcttgccgat gaccacatcg agttcgcgac tgagttcggc
    17641 ctcgtatttc ttgacgatgg cgtcagcctc gggatcggcg gtgacatggg acgaatcaat
    17701 cggcctaaac accggccgcc aggtcaccgt gcgcttgcca tccttttcgg cgatcgtgca
    17761 atacatgtcg acaattgtga cgtaatcgcc ctcctcgccg gattcgacca tggcgactcg
    17821 gttgtcgtag tagatgcgca gatcatgatc atggccggtc agcagcacat cgacatgacg
    17881 cgagcggacg atgttgaaat ccgtcgtcat gtcggtgtgg gtgaccgcca cgacgatgtc
    17941 ggcgccggcc tcgcgcaggg cctttgcctg ctcctgaacc gccgccatct cgtcggtgaa
    18001 gcggaagccg gcggccgagg acatcagcgg ggtggtcgcc agcgcaacgc cgaacacgcc
    18061 gaccttgacc ggccccatct cgaacatgcg cctgtcctca tggccggcga gcggcgcgcc
    18121 atcctcattg cgcaggttgg cagcgaggaa cggcgccttc atctcggcgc ggcgcttgag
    18181 atagacctcc ttgccgaaat cgaattcgtg gttgcccggc acaaacacat cgggcggcac
    18241 cacgttgagt agttccaccg tatgggcacc ctgatcaaag ccgctcatca gcgagggcga
    18301 atacatgtcg ccggcatggg cgtagatcat cgggacgccg gtggcgcgct cggtgcgggc
    18361 gatcgcggcc aggcgcgcaa agccgccacg cccgggggcg gagcccgaca tcttgtagat
    18421 gtcgttgacc agaagcagcc ggaaggtcgg cgttgccttc tgcgcggcag cctgggccag
    18481 cgcctcctcg gcgctcgatg ccaccaccgt ggcgctggca agtcccgctg ctaccgcgat
    18541 ccggaatgtg tcacgccggg taagctcggt catgggtcag gctccattgg tccccgcggg
    18601 cgccgctgtc accccgggca ggttcacatc acgcggccag atcatagaac agccgatttg
    18661 tgatgcaggc aagggctggc aggcggccga aaatatccta catagcgggt atgtcccagc
    18721 ccgttgctcc ccacctgttc cgcccctccg gccaccccga tgtgccttat ggcaagatcg
    18781 gtgtgctgct gctcaatctc ggcacacccg acggcaccga ttactggtca atgcggcggt
    18841 atctgaagga attcctttcc gacaagcggg tgatcgagga gccgaggtgg aaatggtggc
    18901 cgatcctcaa cctcatcatc ctgacagtgc gccccggcaa gaagggcaag gactacgcca
    18961 ccatctggaa caacgaaaag aacgaggggc cgctcaagac catcacgcgc gagcaggccg
    19021 agcagcttgc cgggcgcctc gcaggctatc ccaacatcat cgtcgattgg gcgatgcgct
    19081 atgccaatcc gaccaccgaa agccgcattc gtgcgctgca ggaacagggc tgcgaacgta
    19141 tccttctggt gccgctctat ccgcaatatg ccgccgcgac gaccgcgacc gccgccgacc
    19201 atgccttccg cgcgctgatg aagctgcgct ggcagccggc cgtgcgcgtt gcccccgcct
    19261 atcatgacga tccggtctat atcgatgcgc tggcgcggtc gatccgtgcc catctggcaa
    19321 cgctggattt cgagcccgaa gtcctgctcg ccaccttcca cggaatgccg aagaagtatc
    19381 atctggccgg cgatccctac cattgccatt gcatgaagac cgggcgcctg ctgcgcgagg
    19441 cgctgggctg ggacaaggac cgctatgtga tttcgttcca gtcgcgcttc ggcaatgatc
    19501 cctggctgca gccctacacc gacgagacgg tggaaaagct ggccaagaag gggatcaaga
    19561 ggctggtgat ggtggcaccc ggcttttccg ccgattgcct tgagacgctc gaggaactcg
    19621 acggcgaaaa ccgcgagatc ttcctgcaca atggcggcga gaacttcagc tatatcccgg
    19681 cgctcaacgc cagcggtccc ggactcgatg tcattgagac actggtgcgg cgcgagttga
    19741 tgggctgggt cgaggacatc catgcgatgc cggaaagtga ggctgccatc cgcgccgagg
    19801 cgacggaact cgaccagcgg aacctgctgc cgtgaggcgg ggaaccgtcg ccggatttgt
    19861 ctacggcgcg gccgccttcg cccttgggtt tctgttcggc gttatacggg taagtctggt
    19921 cacgccgcgc atcggtccac tctgggctgt cctggtggaa gtgccggtga tgctggtatt
    19981 gctctggccc ttggcgcggc ggatagcatc gcattggctg gtggctgttg cgttcgctcc
    20041 acgcctggcc atgggaacag cagcccttgc cacgctgctg acggcagaag tcgcgcttgg
    20101 catgatcggg ttcaatcagg ggcttgatga tgtggttggc cgattggcca gtcccgaagg
    20161 gctcttcgga ctggctgggc agatcgcttt cgccagcttt ccctgttggc aaatgcgccg
    20221 agcgatggat tgccgggcac cgtccgccac attgtgcgcg cataggaagc agggcagcgg
    20281 gctggtgtga acgcctccgg cccttgcaat ttactcaacg cggcgtcacc tatcgcatat
    20341 cgtgatcgaa ccacggactg gggagcggac gatggatctg agtggcggcg gtatttttgc
    20401 actcatgctt tttgtctttg ccatcatcac gctgatggcc ggtatcaagc aggttcccca
    20461 gggcatgaat tatacggtgg agcgcttccg ccgcttcaac cggacgctgt cgcccggcct
    20521 ggggctggtc attccctata tcgagaccat cggtcacaag gtcatcatga tggagcaggt
    20581 gctggatgtg ccgacccagg aggtgatcac caaggacaac gccaccgttc aggtggacgg
    20641 ggtggtgttc ttccaggttc tggacgcggc gagtgcctcc tacgaggtcg ccaacctgca
    20701 tcaggcattg atgaacctga ccatgaccaa catccgcacc gtgatgggat cgatggatct
    20761 cgaccagctc ctgtcgcatc gcgacgagat caattccagg ctgctgcgtg tggtcgatgc
    20821 ggcggcttcg ccatgggggc tgaaggtcac acgcatcgag atcaaggata tcgtgccgcc
    20881 cgccaatctg gtcgaggcga tgggtcgaca gatgaaggcc gagcgcgaaa agcgcgccaa
    20941 catccttgag gcggaagggt tcaaacaggc cgaggtgctg cgggccgaag ggcacaagca
    21001 ggcggaaatc ctcgctgccg aagggcgcaa gacggcggcc tttctcgatg cggaagcacg
    21061 cgagcgcaag gccgaggcgg aagccaaggc aacccaggtg ctgtccgcag ccatcgtcgg
    21121 gggcgatact gcggcagtca attatctcat cgccgagaag tatgtcggcg cgctcggcca
    21181 gttcgccacc gccaacaacc agaaggtgat gatcgtcccg atggatacgg cggggctggc
    21241 cggcacgctg gccggcattg ccgaaatcac ccgcaacgct tcgccgggcg gaccgcctgg
    21301 cggtggccag gcagctcaaa ggtcgcaagg cttcggctct gttccgcctc acgggggcaa
    21361 gcctgcctcg ccgttcggca aggtggagta agcggcgatg atgggctcgc tgggtctttg
    21421 gggctggctg gcgctcggct tcgggctgat tgccgtcgag atactgctca ttccgggcgg
    21481 ttttccgctg tggatcgggc ttgccgctct ggcgatgggc ggcatcaccg ccctcgttac
    21541 catgggctgg gaagccgaac tgatcctgtt cgggctgctg tccgtcgtgg cctcggtcat
    21601 cgcctggaag ctgcactacc agcgcagtcg cgcggatgcc gccgacgggc tacacgaccg
    21661 ggttgagcaa ctgatcggcc gcacattcac gctcgaagag gcgatttccg gcgggttcgg
    21721 ccgcgtcagg atcgacgaca cgtcctggcg cgtcagcggc gccgaccagc cggagggcgc
    21781 gaaagtcaag gtcgtcggtg tcgacggcgg cacgttgaag gtagaaaaag cgatatgatg
    21841 atgcctttgg tccacgggtt tgacctgtcg gtcagcctcg gaccatttcc ctgcaaccat
    21901 gcctttggcc ttgctcggcg tcgcgttggc tcgtgaccag ctctgttccg ttctgaccga
    21961 tcggccactc cggaagtccg ctggtgtgat agatgacgta gcagagaaga tagataatca
    22021 ggccacgccc agcctgagca gatcatgcag atgcaggatg ccttcgggcc gccctgcgcc
    22081 atcgaccacc agcacggcgg tgatgcggtt gcggttgagc atttccagcg cttcgacggc
    22141 gagatcgtca ccggagatgg tgcgcggcgc ccggctcatc acctgatcga ccggcagggt
    22201 ggtcagatcg ggccgcatgt ggcggcgcag gtcgccatcg gtgacgatgc cgatgagcgc
    22261 gcctgttgta tcggttaccc cgacgcagcc gaagccgcgc gccgtcatca ccagaatggc
    22321 ctcggccatc agcgtgccgg cggcaacgat cggcacggcg gcgccgacat gcatcacctg
    22381 ccggacatag gtcaggcgtg cgcccagctt gccgccgggg tggaactggc gaaattcttc
    22441 cggagtaaag ccgcgcgctt ccagcagcgc caccgccagc gcatcgccca gcgccatctg
    22501 catcgtggtc gaggtggtgg gcgccagacc gttggggcag gcttcggttg ctttgggcag
    22561 caccagcgcg atgtccgcct cgcgtgccaa agtcgagtcc gaggcagagg tgagggcgat
    22621 caggccgacg ccaaagcgcc gtgaatagga gatgatatcg gccaattctg ctgtttcgcc
    22681 ggaccaggac agtgccagca ccacatcatc cggcgtgatc atgccaaggt cgccgtggct
    22741 ggcttcgccc gggtgcacgt agtgagccgg cgtgccggtg gaggcgagcg ttgcggcgat
    22801 cttgcggccg accagcccgg atttgcccat tcccgtgaca atggtgcgtc cgcgggtggt
    22861 ggcgatgcgg gtcacggcct gggcgaaggg agcgccaagg ccgtcgttga gcgcacgcga
    22921 aagcgcggca agacccttgg tctcgcggtc aagtgtgcgg agcgcggagg cgatcgtggg
    22981 agccgctgct gacatggcga ccggttagca tgaatgggcc cggtgcggcc agtcccgcgc
    23041 caggaaaatc aacggagcgt taaccataag cgtttacgtc cgttaacggt tccggagtct
    23101 cttccggctg ctgctgattc gagagtgccc ttcgacgtga ccgtggccgt tgcccgctcc
    23161 cttctgccgg cgctgctggc actgacgacc gcgctgatcg gcgggccggc gttggcacag
    23221 gtgcgcggca cggtcggtca gtcgacggac cgcctctatg atcccggcaa tgcggatccc
    23281 ggtcgcatgc ggtcgggcac aacgggattc gatgccgatc cgggcctgcc tgcgcggcga
    23341 agcggcaatg caacgcctgc caatcctgcg gaactggccg gcaattcggt ggtcaattcc
    23401 aacacccgcg tcgccgtgcg caagagaacg cgctccgcag cgaccctgac ccggccaccc
    23461 aaccgggctg tcatcgctcc cgcccgcccg gcccagccgg ttgccggcat agcgccgccg
    23521 ttggccgcgc cgcgggccat gccggatatc gttgcgcccg gcctcaccga accgatggtg
    23581 ccgcgcaaga agaagaaaaa ggaagaggat ccctatgcgc cgctgggtct gcgtcttggc
    23641 ggtctcaccg tccatgcggc tgtcgatctg caggccggct atgacagcaa ccccctgcgc
    23701 agcggttccg gcagtgccgt caagggatcg aagttctacc aggttgcgcc tgaggtttcg
    23761 gttgtcagcg actggtcgcg ccatcagctc cagttcgacc tcaagggtgc ctatctgctg
    23821 tttcaggacg tcaacgatgc cgaccggccg acactggatg ccaagctgac cttccgtggc
    23881 gacatatcgc gcgataccac gctcaatctc gaactgaagg aaaaggtcga tacccagcgg
    23941 ccgggcagcc tggacctgac cagcgcggtg aaggggcggc cggctttcta tacccacagc
    24001 ggcagcatcg gcgtcaccca gaaattcggc tatgcctcga tcactgctgc ggccaacgtc
    24061 gatcgcgcca cctatgatca gggcgtcacc tatgccggac tgagctatga tcagagcgac
    24121 cgcaactaca cgacctatgg cgcaaggctg cgcggagcct atgaaattac gccgggaatt
    24181 gtgccctatg tcgaagcaat ggtcgatagc cggcaatatg atcgagagtt caatgccagc
    24241 ggctacaggc gttcgtcgga tggggtcatc ggacgggtcg gcaccacatt cgaggtgacg
    24301 cagcaactga caggggatgt atcggccgga tacgggatgc gccggtatga cgattcacgg
    24361 ctcgatcatc tgagcggtct gttgatcgat gcctcgctgg tctggcagat gtcgcccttg
    24421 accaagatga ccctgaaggg cacttccgag ttcgtcgaaa ccgcgaccgt cggatcaagc
    24481 ggtgcggtca atcgaaaggt cagcgttgat ctggcgcatg acctgttgcg gaatgtgacc
    24541 gtcaccggat cgctcggcta ttcccagaca ctttatcagg gtatcggact gatggagcag
    24601 accatcaacg ctggcctcaa gtttgactac aagatcgacc gcaccttcgt tgtcaggggc
    24661 agttatgcct atgagcgcgg gctctcggac aatgccgggc aaagctacct cgcccatgtt
    24721 ttcctgctgg gcctgagaat ccagcgctga gccggtaacc cgcttgttca ggaatttcag
    24781 cggcaccgat tgggcggcgc ctatcgtgat tgcacagatg aaatatcccg gacctgtcct
    24841 tgcgttgtcg catacagcgt taacaccata ttagctcatt gccgcaatga tggttctgct
    24901 gatgccaaaa tctcatcctg cgggaaggcg actcgcgggg tgatcaaaaa caagagcgcc
    24961 aaagcttaaa agctacaatg cctcatcaac acacattggc cgatgccggc atcacccttg
    25021 tcgagaatgc agccgaggag agctttgaca aggtcaaggt cctcgtctca tctctgtttt
    25081 cgtcgcccgc atcgctggct tcaggcgcgg ttgcggcttt ggcgaccggg gcaatctgcc
    25141 tttatgcaag tggcgaaatc ctgttcgtct attggctgat cgctgcgctg gcgatcaatc
    25201 tctaccggct cgcattcact cggtttttca acactgccgt cggcgacatc acgaccattg
    25261 ccatgttgcg ctattacgag cgccattttg tcgttggggc catgttgcag agcctgctga
    25321 tgggcacggc agcggtgatt gccattctga tgacgcacag cgatgcggcg cagattgcct
    25381 cggtgacctc ggccattgct ttcagttccg ggtttgtggc gcgcaatgca gcccgcccgc
    25441 gttttgttgc cgctcaattg ctggtcacgg ttggcccgct gaccgtcgcc ttcttcatgt
    25501 cgtcgatgcc ggactacaat ctgatcggtc tgttcgccat cctgttcatt gtctcaaatg
    25561 tcgtccagac ccgctcggtt caccgcaatc tggtcgcatt gtcggtcgca accgcccgtg
    25621 aaaaggaact gacccaggca gtgcgcgaga aaaaccgcat gctggacatc gcgctggctc
    25681 atatgagcca ggggctggcg ctgttcgatg aggccgggcg cctgaccgtt tccaaccagc
    25741 gctttctcga tttcctgtct gtcagcgacg ccggcaacac gcacgcgctg ccggccaacc
    25801 agctggcctc gctgctggtt gtgcgtggtg tgcttgaccg tgtttcagcg cgtgccctgc
    25861 acaaggcctg gaccgacatg cagcagaacg gcggtcggcc acgcttcgat ctttcgctga
    25921 agagcggaca tatccttgat gtcattctga cgcccgtcga gggtggcggc ttcctgatga
    25981 cttcggaaga tgtcactgcg gcgcgcaagg cacaggcacg gatcgagcat ctggcgcgct
    26041 ttgatgatct gaccgggttg ctcaaccgcc atgaagccaa tgtccgcctc agccaatggt
    26101 gcgaccgccc caattccttt gccgttgcct atctcgatat ttgccagttc aaggaaatca
    26161 acggccgttt tggccatgag gcgggcgatc tcaccctcca ggccgtcggt cgccgcctgc
    26221 agagcttcgt cagggatgac ggatttgttg gccggctcgg gggtgacgag ttcatcgtcg
    26281 tcctggatgt caccgattca gcccatgcca tgcgcctgct ttcccggctg tcgcagagtc
    26341 tcgaacggcc gattatgatc gagggacgtt cgctcaaggc tccggtcaag atcggctttt
    26401 cgatgttccc cgatcacggt acgacggcgc tgcaactgca gcgggccgcc gatttggcgc
    26461 tgtatcgggc caaggagaag tcgattggtc cgatggccta ccttaccgag atggggcagg
    26521 ccgcggctcg cctgcgtgac atggaagagg atctgcgcaa ggccctggtc aagggcggcc
    26581 tcgaactcta ctatcagccg atcatcgatc tcaataccaa gacgatcgtg agttttgagg
    26641 cgctgatgcg ctggaaacat ccggaacgcg gcttcattcc gccgtcggaa ttcatcccgg
    26701 ttgctgaaag cacaaatctc atcgtcgaac tcggcgaatg ggccatccat gaggcggcgc
    26761 gctcgatcgc gacctggccg cgtcacgtct cggtcgcggt caatgtctcg ccgatccagt
    26821 tcgcctcggg ggagcgcctg ctggaggtgg tccgttcggc ccttgcggaa agcggcattc
    26881 cagccagcca gctcacgctg gaagtgaccg aatcggtgct gatcgatgat tctggatcgg
    26941 tgcgcgatac gatgatcaag ttgcgcgaca tgggcgtgaa atgtgcgctt gacgatttcg
    27001 gcaccggcta ttcgtcgctg agctatctgg cgtccttccc gttctactgc gtcaagatcg
    27061 accgctgctt cgtcaaggat atcgaggcaa gcagggcatc gcgcgcaaca gtggaagcgg
    27121 tgtgtcatct cgcccgcagt ctcggcattc agacagtcgc cgaaggtatc gaagaagcgg
    27181 cccagggcga tgttctggcg cagatcgggg tcaaccgcgg gcagggctac cattacggtc
    27241 gaccggcgcc ctggagcgag gcctcggcgc ggctgagcca ggccgcatga gagcaaaatc
    27301 ccgaattccg ggacattgaa gcggttggga agcgacgggt gcgttccttc tgccgactat
    27361 acaaccgttg gctgttctag tctgctgttt cattccgaaa cagtcccggt agcgttaact
    27421 ccgttttaat ttttgcaaat agtaacgttt acaaattgca tcactcgagg cagtgtagct
    27481 gctccaaggt agctggagtt tggtccgggt gccgaagttc gcgagtgttt caatgtccgt
    27541 tgctgcatcc attgcattta agcaatcagt ttcttctgat gtccttgatg caatttatgg
    27601 ttgggctggc ccactgccgc aggtcgaact ggagttcgag gccgagctga aactgctttg
    27661 gctgacgatc cgtccggaac ccaagccgat cttcaccctg caactgctcg acagcgttgt
    27721 tgcggttcag aaggcgattg gccatctgtg ggcggatctg cggccacacg accggccggt
    27781 gcggtttctg gcattccgcg gccgcggcgg cgtgtttaca cttggtggcg atcttgattt
    27841 ttatctcgaa tgcatcgcca ccggtgaccg gtcgaggttg atggactacg ccgaggcttc
    27901 ggtccagggg gcgatcggca atgcgtcggg ctgctatggc tcggtgatca cgctttcgac
    27961 gattcacgcc aaggccctgg gcggcgggat cgatgcgccg cgctcatgcc acatcatgat
    28021 tgccgaacgg caggccagct tctgctaccc ggaaatccgc ttcaatcatt tcccgatcac
    28081 ggctgtgccg gttctgtcgc gcaagcttgg gccgatggcg gccgaagcgc tgctgttgtc
    28141 gggcgacgag atttcggccg aagaattccg tgcacgcggc ggtctcgaag cggtggtgga
    28201 ggccaatcag ggcgaggact ggatccgtaa ctattgccag acaaatcttg aaagcgcggc
    28261 agcacggctg gccatctacg gcatgaccta cgaggcgaac cgccactacg agcaggattt
    28321 gcgcgacagc gcagcccgat gggtgcaggc catgctcaac atgcgcccca ccgagatctc
    28381 caagctgcag cgcatcagcc agatgcagga gaagctgctg gcccgcatgc gtagctgatc
    28441 cagggtcggg accctggccg atcgcgccgg aacggtgggg caaagccttc cgtccggcgc
    28501 ctccggtgcc tcaagtcgcc cgccgccccg tcctgccgga caggttaccg gagaggtctt
    28561 gcgggatcat ttgatcaggc cgagaccgga ggctgacaag tccagcgaca gggttgcgat
    28621 aaaggctgct ccgcaccatt tcgactgccc cgatccggaa acgatgccgc gcggatcgga
    28681 ggtgttgaga aagcagttcg ccttcgacag atccgtatca tgatagcgca gatccagtgt
    28741 cgctgccttg taggtatagg aaacgccagc attccaatag gtgtagtcct tcaggtccag
    28801 cccgccgaga taggcgctgg tcttgccaag tgcgtagcgg ccaagctcgc ctgagacggc
    28861 cagcccttcg atcggcagcg gcggaaccac cttgagcgtg cctgaataat aggtaccggt
    28921 cgcgccggtg ccgagccagt tcggcgaatg gaacaggttg gcgccgatga aaaccttatc
    28981 ttcccacgaa tagagcgcct tgccggcgat ctcgtaaaaa tcggtattct tcggcgtcca
    29041 gatcgcgccg aagccgtcga catactgctt ttcgcgcggg taatagtagt ggatcatgcc
    29101 aatgtcgaag gtgaatggtc caaccttcgg gcggatgcca taggtgaagt cgatctcggc
    29161 gagcggcttt gtcggcaggt cgaccgaata gccggcgatt ccggcataga gccactgata
    29221 ggtcagttcg gcatagccct ggacgccggg tttgcggttg gtctggctga tgccgcggaa
    29281 gttgtagtcg gacacgatgc gggcgccgaa ggtcatatcc caggccggtg cggcggcagg
    29341 ggcaaccgga ggcattgcct tcttggattt ctccagatcg gcgccggtgg ccgtgcccgc
    29401 ggcgagcagg gctgacgcca gcaggacggg gcggaaaagg ctattcttgg gagcagtcat
    29461 ggtcatctcg cttcctgttc gcgatccctc gcatgtgcga gtcatcatgc cttgacaaag
    29521 cgcgcggggc cgcgaaggag caatgtcaat gggtgggcgc cctgcctgac cgttgggcaa
    29581 cgggctgcgc ctctgcccaa gccgcgggca ctgcacgaca aatctgaacg tctgttgcga
    29641 gacggccaca ggcgctgcgc cgccgtttta ctgccattca ggccgattgc agaacaatgg
    29701 aaccctgatg atcggaagcg ggcttgggag ggattcatgg ccggatcagg caggcaggcg
    29761 gacagcctga cgggcgtctg gcatgggcaa tatgcctatc cgctgctgct ggagccggtg
    29821 cagttcaccg ctacgctgat cgaatccggc cggcagatca gcggctcgat tcatgaaacg
    29881 ggtcgtcggc cgggtcaggt tgcgcagcag ctcaaggcca tgctggaagg cgcggtcggg
    29941 ggcgccactc tgcgcttcgt caagacctac ctgccggcat ccgaccagca ccaggacgtc
    30001 atctacatcg gcacgatcaa tgccgagcgc accgagattg aaggggaatg gatcatcccc
    30061 ggcgattggt caggcaggtt cgtgatgatc aggatgcgcg atccggccca gtccgtcgag
    30121 cagcgacaga tgatcgaggc gtagggccgg cgctggaatc tgcgtgacag ggcgctggtt
    30181 gtcggttgac ttcagagccc ggcgatccgc ctgatttcgt cgagaaattc gtcgcgcagt
    30241 tcggcgcggt cgagcccata ggcgatctgc gccgaaagga agccgatctt ggagcctgtg
    30301 tcgtaggtcc gcccgtcata gtgcagaccg aagaatttct ggcctgccat gagcttgatc
    30361 atggcatccg tgagctggat ctcaccgccg gcacccttct ccttggccga aaggatatcg
    30421 aagatttcgg gctgcaggat gtagcggccg gagatgatgt agtttgaagg cgctgtgccc
    30481 ttgggcggct tctcgaccat gccggtgagt tcgtgcacgc gaccgtgggc ggcgccaacc
    30541 gcgacgatgc cgtattgatg ggtctggtcc atcggcacct gctcgagcgc gatgatgttg
    30601 ccgccgtgac gctcgtaggc agcgaccatc tgtttcatgc agcccttcga cagcatgtcg
    30661 ggcagcatca cggcgaaggg ttcatcgcca acgatgtcac gtgcgcacca gaccgcatgg
    30721 ccaaggccga gcggcgcctg ctggcgggtg aaggtcgcca tgccggccgg tggcaggtcc
    30781 ttctgcagct gttcgagttc cttgagcttg ccgcgttcct tcagggtcgc ttccagttcg
    30841 taggcgatgt cgaaatggtc ctcgatcacc gccttgttgc gacctgtgac gaaaatcaga
    30901 tgctcgatgc ctgcctcgcg tgcctcatcg accacccatt gcagcaccgg gcgatcaacc
    30961 accgtcatca tttccttagg cacggacttg gtcgccggca gaaaccttgt gccgagtccg
    31021 gccacaggga agacggcctt gcggattttc ttcacgctca ttgttctgtc ctaagtgcag
    31081 ttggcagcga agcagaataa gcggcgatcc tttgcagatt tgcacttaaa gagcgccggc
    31141 ggccttgtct gccgcaaact tgcttaacga tccttgaact gtcggagggt atcacagcag
    31201 gggagcaatc ttttctgtcc atagttccca tccggtaacc ggacaggcga cagatttagg
    31261 agtggtcatg acagttctgg taacaggtgg agctggctat atcggcagcc atatggtgca
    31321 cgcattgcgt gaccgcggcg aacgggtggc tgttctggac aacctttcca ccggctttgc
    31381 ctgggcggtt ccgccggatg tggccttctt cggcggcgac gtggcggatc aggatctggt
    31441 caaacgcatc atacgggacc aggaagtgac cgctatctgt cacttcgctg ccaagatcgt
    31501 ggttcccgat tcggttgccg atccgctcgg ctactacgcc ggcaacacca tggcctcgcg
    31561 caacctgatc gacgcggcga tcaaggccgg cgtgaatcag ttcatctttt cctcgactgc
    31621 ggcgacctat ggcgagccgg aaatcatgcc ggtctccgaa gatacgccgc aggtgccctg
    31681 ctcgccctat ggccgttcga agctgatgac cgagtggatg ctggaagatt ccgcccgggc
    31741 ctacgacttc cgctatgtcg ccttgcgata cttcaatgtt gccggcgccg atccattcgg
    31801 acggacgggg cagtcgactc ccaacgccac tcatctgatc aaagccgcat cgcaggcggc
    31861 gatcgggctg agaaagcacc tcgatctcta cggcaccgat tatccgaccc cggacggcac
    31921 ctgtgtgcgc gactatatcc aggtgagcga tctggccgat gcgcatgttc tggcgctcaa
    31981 ctacctgcgt tctggcggga tcagcacggt tttcaactgc ggctatggca agggcttttc
    32041 cgtgcgggaa gtcatcgaca tggtcaagaa ggtcagcggc gttgattttc cggtcaacgt
    32101 tcagggacgg cgagccggcg atgctgccgc catcgtggcc aaggcagaca gcattcgcca
    32161 gaagcttggc tggatgccca agcatgacga tcttgaagag atcgtgcgtc aggcgctcag
    32221 gtgggaacag cacctgcgca gccgcaatgc tgcctgaacg cttcggaccc tggggtagga
    32281 ggctgacatg ccgagcctga cgcgcgcgct ccgatcgctt gtgatggctg tcgttctgct
    32341 ggcgggccct gcggctctgc cggcgcgcgc cgatttcaag gcattcgtcg caggcctgtg
    32401 gcccgaggca caggccgccg gggtgtcgcg cgccaccttt gatgctgcct ttgccggcgt
    32461 cacactggat gcgagtgtgg tggcgcagac caaaaggcag tcggagttca tccggcccat
    32521 ctgggactat gtcggcagcg cggtttcgac atcgcgcatc agccgcggcc gcgccatggc
    32581 ggagcaatgg agcgaaacgg ttgcggcggt cgaacgccgg tatggcgtcg atcgccgggt
    32641 gttcctcggc atctggggca tggagacgag ctacggcagt ttcttcggcg gccattcggt
    32701 gatccgggcg ctcgccagcc tcgcctatgt ccgataccgc gacgacttct tccgcaagga
    32761 gctgatcgtc gcgttgcaga tccttgagga aggccatgtc gagaaggccg acatgaaggg
    32821 gtcgtgggca ggggcgatgg gccataccca gttcatgccg tcgagtttca tgaaacacgc
    32881 ggtcgatgcg accggcgacg gacacaagga catctgggga tcggtgcccg atgcactggc
    32941 ctcgaccgcc aactacctca agcagcacgg ctggcagagc ggtctgccct ggggcttcga
    33001 ggtgatcctg ccggaagggc tggacttcaa gcaggcgctg gggcgcaagt ccttttccgg
    33061 ctttgccgcg ctcggcatca agcgggccga tgaagaggcg atgccgtccc gcggcgaggc
    33121 aaacctgttc ttgcctgcgg ggatcaaggg gcccaaattc ctgatcaccg acaatttcaa
    33181 ggtcatcaag gcctacaatt cctccgatgc ctatgcgatg ggcgtcggcc tgctcggcga
    33241 taccatctac ggcggtgagg gattgaccgg gcgttggccc aaggccgaca agcggatcac
    33301 caaggccgaa ggtgtcgcca tccagaagct gcttaaccag cgcggcctct acaatggcaa
    33361 gtatgacggc cgtttcggtg aacagagccg cgaggcggtg acgcgctatc agctttcggt
    33421 gggcatggtg cccgatggct atgctacgcc agcgctgctg gcgcgactga aggggtctcg
    33481 ctaaggcgcg cgcctcagcc tatactggca ccatgatacg ccagcatcat cgttatggga
    33541 cgttgctgac tgccttgctc gtgctcgtcg ttgtctggtc cggagcgatc ggcgaggcgt
    33601 tggcgcaagg tgacggctcc tcggtctata cgacagcgcc gaagggccgc aatgatcgtg
    33661 gtccgctcga ctggctgttc ggtcgccgtg gcggcagcgt cacgccggtg ataccgcaag
    33721 cggcgccgcg cgccgattcc aagccaagaa cctatcgtcc ggcgcggcgg acaacaccgt
    33781 caaccctgcc gactgtgccg ggcgatccgg cagcaccggc ggacaccaat gcgccatcgg
    33841 ttgcgggtga tcctgcggcc cctgcgccgg ctccgccgcc agcgctgcca ccggtttcag
    33901 ttgtggtggc tggcgattcc ctgtcggtct tcctcggaca gggcctgcag gagctctatg
    33961 ccgagaagcc gcatgtcagc ttcgtcaagc gacatcgtga tgcgtccggg cttgtccggg
    34021 acgacttcca tgattggccc aaggcgttcc gagacctggc cgccggtgcc cgaccggatg
    34081 ccctggtcct gatgatcggt tccaacgatc gccagcagct tcgtgatgac cagggcgtgc
    34141 acgaaccgct gacgccgcgc tggaaagaac tctatgccaa gcggatcgac gatgttgtcg
    34201 cagcgatcag ggggcagggg attccactca tctgggtcgg attgccggtg atgcgttccg
    34261 agaaatacag tgttgatctg acggccctca acgaccttta ccgggcgcgt gcccaggctg
    34321 cgggtattgc gtttgtcgag atttgggaag cattcgccgg agaggatggt cattacacgg
    34381 tcaatggtcc tgatgtcggt ggcgagatcg tgcggctgcg gacctccgac ggggtgcatt
    34441 tcaccaaggc gggagcgcgc aagctggcat ttttcgttga ccaggagctg gcaaagctgt
    34501 tgggcgcgat caagccgcag ccgccccttc cccagcagcc ggccacgccg caggttgacc
    34561 caggagatct gccgccgcca cctcaggtga tggcccctcc tggcccggag cgttttccgg
    34621 cacctgccag tccgtcgttc gacgcgatgc ttggcgtgcc gatgccggag attgccgccg
    34681 ctccgactct ggttccgcgg ccggcccagg gaccagtgat gccgctcaat gccgcgccgt
    34741 tgacaaccgg tggacagctt ttgtccgcac agacggtcaa ccggcttgat gaggcgagaa
    34801 aagtgttcgt gcagggcgtc ggtgccccgc ccaagccagg tcggctcgac gacttcaggc
    34861 cgcccgagcc atagagcatg gcgcgattgg gtggataccg gtcaatcgca gaagacatgc
    34921 tctaacttat tgataccgat cacg
//
LOCUS       DAOMXG010000006        32213 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_931_length_32213_cov_9.419553, whole genome shotgun sequence.
ACCESSION   DAOMXG010000006 DAOMXG010000000
VERSION     DAOMXG010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 32213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32213
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_931_length_32213_cov_9.419553"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(246..3992)
                     /gene="metH"
                     /locus_tag="PLQ11_00925"
     CDS             complement(246..3992)
                     /gene="metH"
                     /locus_tag="PLQ11_00925"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012453549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HRK23493.1"
                     /translation="MTQSPVTHVDGDDVRRALNEAARQRILVLDGAMGTEIQQLKLTE
                     EDFRGSLFKDHSHPLKGNNDILILTQPAAVRKVHLDYFLAGADIVETNTFSGTTIAQA
                     DYHLEGIVSELNFHGARLAREAALLAEKHDGRRRFVAGAVGPTNRTASISPDVNNPGF
                     RAVTFDDLRIAYAQQITALIKGGSDLILIETVFDTLNAKAAIFAAEQAFAATGRRLPV
                     MISGTITDLSGRTLSGQTPTAFWHSLRHARPLSFGLNCALGAREMRGHIAEIAKVADT
                     LVCAYPNAGLPNEFGLYDESPEAMAALVADFAKAGLVNIVGGCCGSTPAHIAAIANAV
                     AGLAPRAVPEIAPALRLSGLEPFTLTPDIPFVNVGERTNVTGSARFRKLIKDGKYPEA
                     LDVARDQVANGAQVIDVNMDEGLLDSEAAMVTFLNLVAAEPDIARVPVMIDSSKWNVI
                     EAGLKCVQGKPIVNSISMKEGVEAFIDHAKLCRAYGAAVVVMAFDEQGQADSYERKIE
                     ICQRAYKILTEEVGFPPEDIIFDPNVFAVATGIEEHNGYGVAFIEATRWIRQNLPHAH
                     VSGGVSNLSFSFRGNEPVREAMHAVFLYHAIRVGMDMGIVNAGQLAVYDALPAELRDL
                     CEDVVLNRRADATERLLEAAPRFKGDGKAEVKMADLAWRALPVEKRLEHALVNGITEF
                     IEADTEEARSAAARPLHVIEGPLMAGMNVVGDLFGAGKMFLPQVVKSARVMKQAVAYL
                     MPFMEEEKRRTGADQRASAGKVLMATVKGDVHDIGKNIVGVVLQCNNYEVIDLGVMVP
                     AERILEAARKERVDVIGLSGLITPSLDEMVHMASEMERQGFDLPLMIGGATTSRVHTA
                     VKIAPAYQAGQAVYVVDASRAVGVVSSLLNTDSGAGYKQALRAEYSKVAEAHARAEAE
                     KQRLPLARARANAFNADWANYTPPKPTFTGTRVFKTYGLAELVPYIDWTPFFQTWEMK
                     GRFPAVLEDEKQGAAARQLYEDAQAMLTRIVEERWFQARAVIGFWPANRVGDDIRLYT
                     GESRRDTLATLHTLRQQLTRRDGKPNLALADFIAPEGVKADWIGGFAVSTGFGEERIA
                     ERLKGRNDDYSSIMAKALADRLAEAFAERMHERVRREFWGYAADETVTKDGLLLEDYR
                     GIRPAPGYPAQPDHTEKATLFSLLGAERRIGVKLTDSYAMWPGASVSGLYFSHPDAYY
                     FGVAKIERDQVEDYAQRKGMAVTEVERWLGPVLNYRPLDGAAEE"
     gene            complement(4023..4922)
                     /gene="metF"
                     /locus_tag="PLQ11_00930"
     CDS             complement(4023..4922)
                     /gene="metF"
                     /locus_tag="PLQ11_00930"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HRK23494.1"
                     /translation="MIAPLLRHSRSPSTQPPRVSFEFFPPKNDEMEATLWASIQRLAP
                     LDPSFVSVTYGAGGSTRERTHATVRRIIEETRLRPAAHLTCVAASKAEVEEVIRAYHA
                     VGVRHIVALRGDPVEGVGHAFRPHPDGYRSSAELVAGIKAIGDFDVTVSAYPEKHPES
                     ASLDEDLDILKAKIDAGADRAVTQFFFDNDCYFRYLDRVRARGISIPIVPGILPVQSF
                     KQAAGFATRAGASVPDWLAQRFEGLDRDAETRKLVAAAVAVEQVYDLLDRGVSDFHFY
                     TMNRADLVFAICHMLGLRATSAA"
     gene            complement(4922..5929)
                     /locus_tag="PLQ11_00935"
     CDS             complement(4922..5929)
                     /locus_tag="PLQ11_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRK23495.1"
                     /translation="MPASLPFPAAISALEAAGEPTRLRILALVSQAELTVTELMAILG
                     QSQPRVSRHLKLLVEAGLVERHKEGAWAFFRRSDAASAAMLAGEILRWIDPGDVTVAE
                     DRRRLAAVRSERARQAAQYFAQHAEDWGRIRAMHVPESRVEAAILDILGDIRIEAMLD
                     IGTGTGRMLEILAPRARRAVGLDVSPAMLAVARANLERAGIGNVQLRQGDAYALAPGE
                     GGFNLIVIHQVLHFLDDPVRAVREAARLLAPGGRLLIADLAPHADERLRSQFNHRRLG
                     FAPEEIASVLRDAGLEAIRVRALKPLPGEGDVLTVMLWLGKDSRIQTDAPFSKQSVEV
                     A"
     gene            6125..6820
                     /locus_tag="PLQ11_00940"
     CDS             6125..6820
                     /locus_tag="PLQ11_00940"
                     /inference="COORDINATES: protein motif:HMM:NF018755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1353 domain-containing protein"
                     /protein_id="HRK23496.1"
                     /translation="MRSLKILAVLSFLMIGTAQAAFIGTFELVDNPGGKDGKLKKLKN
                     DFAFKDKSGVGWEAAAGLETDGASIPFWAQPVIGQPYDGSYIKAAVLHDHYCDRKVRD
                     WKKTHRMFYDALIEQGVPKIKAGVMYGAVMIGGPTWTEVNVGKKCKLTDVCIRTVGGD
                     RARMVWNQRPAVYDQIGDLAERLRKIEQRLNAAGDFSAEDVEAIAVDLAPENSNIRQP
                     AHAELEEISLPTK"
     gene            complement(6824..8287)
                     /locus_tag="PLQ11_00945"
     CDS             complement(6824..8287)
                     /locus_tag="PLQ11_00945"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018044964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HRK23497.1"
                     /translation="MKSGGFIEVASPGEAVDALIGLHGQATGALRQALGRYLETRRPP
                     SAAERAAFRYPELRLTYEPDGVPHSTRRAYAKFQRVGVYSTTITQPDAFAPYLLEQLQ
                     PLVAEYGARMQVGLSRQEMPYPYVVQAGDELGRAGIGPAELARFFPVPRLSAVGDEVA
                     DGTYQVGENEPWPLALFDAARVDYSLKRLVHYTGSDWRHVQPYILLTNYHRYVEQFVR
                     WGMAQLGGATRFSKMVLPGNVEVKEPGTGDAGDSLIDISAWSKFQMPAYHLAGEGGGI
                     TLVNIGVGPSNAKNITDHLAVLRPHVFLMIGHCGGLRQSQEIGDYVLAHAYLRQDRIL
                     DALVPPEIPIPALAEVQVALQEAAAIVTGERGEGLKRRLRTGTIVTNDDRNWELRWSQ
                     ERHRINLSRAIAVDMESGTIAANGYRLRVPYGTLLCVSDKPLHGEIKLPGAASAFYER
                     AVGQHLQIGLKAVDLLRGKLDQLHTRKLRSFEEPPFR"
     gene            8452..9300
                     /gene="panC"
                     /locus_tag="PLQ11_00950"
     CDS             8452..9300
                     /gene="panC"
                     /locus_tag="PLQ11_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012496579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HRK23498.1"
                     /translation="MAGPKVLSSVADLRAAVAGWRRAGETVALVPTMGALHAGHVSLA
                     RMAQGKARRVIVSIFVNPTQFAPTEDFSKYPRTFAADCAKLAEAGVDAVYAPTPEVIY
                     PQGFVTTVSLAGPATAGLEDKFRPTHFAGVATVVSKLHIQAMPDIAIYGEKDFQQLAV
                     ITRMNRDLDLPITVLGGPTVREADGLALSSRNVYLSSADRKVAPVLHATMQDCARRLR
                     SGADLETVLAAGRAAITSAGFALDYLELRDAASLGPADPGAGHPLRLLVAARIGATRL
                     IDNIAV"
     gene            9505..10410
                     /gene="prmB"
                     /locus_tag="PLQ11_00955"
     CDS             9505..10410
                     /gene="prmB"
                     /locus_tag="PLQ11_00955"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HRK23499.1"
                     /translation="MTNVPPELATLRDFLRYAVSRFRAAKLAFGHGTANAHDEAAFLL
                     LEALHLPIDRLDPYLDARLTGDERQRIADLIEARITTRKPACYLLNKAYIQGIPFYVD
                     ERVIVPRSYIGEMLMGGLFEDGPLADERDPAEVGRVLDLCTGSGCLAILAAHVFPNAT
                     IDAADLSRDALKVARRNVDEHGLEDRVALIESDLFSALKGRRYDLILTNPPYVTAAEV
                     AAFPPEYAAEPQMAHLGGADGMDLVRTILKDAKKHLNPGGGLLCEIGLGREIIESEYA
                     DIPFLWLDSEESEGEVFWLQKEELP"
     gene            complement(10422..11342)
                     /locus_tag="PLQ11_00960"
     CDS             complement(10422..11342)
                     /locus_tag="PLQ11_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRK23500.1"
                     /translation="MSNDQTGTGRTIQLFLVDGVPNGLIIASIHGWTGSILVSTQSTF
                     AKLLARPEADRTGIYVLFGPDPDDPLSMRAYIGEGDSVRERVGQSAKTRAFWETVAVV
                     TTSDEALTKGHVRYLEARLIEMAVMAGRVTLDNGNAPSADRKRLPEADRANMEAFLEN
                     LKVVLPVVGFEFLKPKATRTLATDSSLGDDRSQFEIRHKSGIKAFALEIDGEFIVRKG
                     SEALKDAGYKHNSSYGELRKELIEQGKMVPDPAGKRYVFVEDFPFRSPSAAAAVVLDR
                     NSNGRTEWKVVGEKNLTYDDWQIARSNSPL"
     gene            complement(11346..12998)
                     /gene="ettA"
                     /locus_tag="PLQ11_00965"
     CDS             complement(11346..12998)
                     /gene="ettA"
                     /locus_tag="PLQ11_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HRK23501.1"
                     /translation="MSRQFIYHMRGLSKTYPGGKQVLNNIHLSFYPDAKIGVLGVNGS
                     GKSTLLKIMAGIDKEWTGEGWVAEGARVGYLPQEPRLDETRTVRENVMLAVAREQALI
                     DEYNALMADYSDENAEKAAKIQDVIDAENLWDLDSRIDQAMDALRCPPDDWQVDKLSG
                     GEKRRVALCKLLLEKPDLLLLDEPTNHLDAETTQWLENHLREYPGAILIVTHDRYFLD
                     NVTGWILELDRGQGIPYEGNYSSWLTQKQKRLEQEGREEAARQRTLARESEWVAASPK
                     ARQAKNKARIQRYDELVQRAEEKTAQTAQIIIPIAERLGGNVIDFEGLSKGFQDKLLI
                     DDLSFKLPPGGIVGVIGPNGAGKTTLFRMISGTEQPDGGKIVIGDTVQLGYVDQSRDS
                     LDDKKTVYEEISGGNEVIYLGKKEMNARAYCGAFAFKGGDQQKKVGMLSGGERNRVHL
                     AKMLKKGANVLLLDEPTNDLDVDTLRALEEALEDYAGCAVIISHDRWFLDRIATHILA
                     FEGDSHVEWFEGNFADYEEDKKRRLGPDSVLPKRINYRQFTR"
     gene            complement(13088..14395)
                     /gene="chrA"
                     /locus_tag="PLQ11_00970"
     CDS             complement(13088..14395)
                     /gene="chrA"
                     /locus_tag="PLQ11_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018263577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HRK23502.1"
                     /translation="MRQGAATASLAETFRAFLTIGLMSFGGPAGQIALMHRMLVEERR
                     WVSEDQFLHGLNFCTLLPGPEAMQLATYIGWLRHGVLGGLIAGVLFVAPGFCVILALS
                     MLYAQFESQTWFTALFFGLKAAVLAVVLEALVRVAKKSLRGGWAHAIAAASFLALFAF
                     RVPFPLVVLAAGIAGWALAGRLAGVSASGTLPAADVPVPGWHHIVTTLAIWGGLWAAL
                     PLAMIALFGAGTVFPALAVFFSKMAVVTFGGAYAVLAYVAQAAVETYGWLKPGQMIDG
                     LALAETTPGPLILVLSFVGFLAAYQAPATLPPLLAGVLGAVMTTWVTFIPCFLWIFLG
                     APFLERVRHNPALKGALAGIGAAVVGVILNLSLWFALQTLFARVGEVAAGPLRLHWPE
                     WTSLDGQAAVLTILAMVALFRLHLGMIPVLGLCAVLGVALRLI"
     gene            complement(14392..15207)
                     /locus_tag="PLQ11_00975"
     CDS             complement(14392..15207)
                     /locus_tag="PLQ11_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase/chromate resistance protein"
                     /protein_id="HRK23503.1"
                     /translation="MPFDPRLSTKDLFLSLGRAEAPLIVDVRIDDDFAADPRRLPGSV
                     LCSHSSVKDFMARRSDRVVAVVCQRGLKLSEGAAAMLRLDGITARVLEGGFEAWKKAG
                     LPLVPEGENPLAGGAARSLWVTRERPKIDRIACPWLIRRFIDRNAEFLFVRDTEVAAV
                     AERFGAIPFDVEGVRWSHSGPECTFDTMIRAFDLMHEPMQRLAAIVRGADTARLDLAP
                     QAAGLLAVSLGLSRLFSDDLAQLDAGMLIYDALYRWCVEASGETHNWPAGKTQ"
     gene            15619..>16221
                     /locus_tag="PLQ11_00980"
     CDS             15619..>16221
                     /locus_tag="PLQ11_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23504.1"
                     /translation="MTASAQLQPQQPARVLVVAQDAASVASLTSEMMRRSALVAIAAT
                     VAEAGAAVRSNGFDVIFVAAREADAETALLIQLLRGASQIAPRILFLAHPDHIADHPM
                     AVAAADATISWTLPALEIADLAVPRAEPPKKKPAQIITKPSRKTRTAEIEAADDETAA
                     SIDAALIPAAETIARAAEAIARAAESIASRQMQASERPAET"
     assembly_gap    16222..16231
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16232..18899
                     /gene="bcsA"
                     /locus_tag="PLQ11_00985"
     CDS             <16232..18899
                     /gene="bcsA"
                     /locus_tag="PLQ11_00985"
                     /inference="COORDINATES: protein motif:HMM:TIGR03030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016759 - cellulose synthase activity
                     [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HRK23505.1"
                     /translation="LTGSAEVEDIPSPAEPVSAPVAEVSVEQPLVQAPAVPRAPAPAP
                     HLAAVSTPPAPAPVTPPALREAPKPHVATVARLPQRPVHMPELPPKPAVEAVPEPQGE
                     ARQNRRFYDAKDAAPKHSRASKLFFATGWFLLAGAIVGLTTWPISTQTQFTLGLVVLA
                     AMTVIRKLRLGGIWRPTFLALGITMILRYVYWRTTSTLPPMHDWVNFTPGFMLYLAEM
                     YCVLMFFISMFIIADPIERKPPPDLPDDRLPTVDVYIPSYNEGADLLAATIAAATQMD
                     YPKDKLNVYLLDDGGTDQKCNQSDPEKAAEAIARRAELQSLCLEMGINYITRARNEHA
                     KAGNMNNALQQTSGQIIVVFDADHAPARQFLRETVGYFALDRRLFLVQTPHFFINPDP
                     VEKNLQTFDRMPSENEMFYGTIQKGLDKWNGAFFCGSAALISRRALESVGGFSGISIT
                     EDCETALDLHKRGWNSIYVDKPLIAGLQPESLVSFIGQRSRWCRGMVQILVLKNPLLA
                     RNISLKQRICYVSSMFYWLFPIPRMIFLLSPFLYIFFSMQIFQANAQEFFAYVSIYML
                     THMSIQNYLHGSTRWPWISEAYEYVQTIYLVKGIWGVLLNPRKPTFNVTAKGLTLDND
                     HLSELAWPYFAFFGLILLSLVVAVSRFLAENEFNELLLIVGGWSFYNLLIAGVALGIV
                     AERRERRKTPRLVIERAGELHVGEHIVPVDIQDCSIGGMRIKPKEGRLPEGYGRGTPA
                     FIHVLPTRPDFDTDAIPCTIQNASNDGKGAVYGLQFRELRPVHFRGIADAMYSDMAVF
                     ENFRMSRRKIKGITMGTLQFLWWSMHHTVRAFYYMIALRNVEAPKVQDGEQMAPTEYK
                     GAHPDFHEPATPVDITHPQPEAIIARPVITAH"
     gene            18912..21467
                     /locus_tag="PLQ11_00990"
     CDS             18912..21467
                     /locus_tag="PLQ11_00990"
                     /inference="COORDINATES: protein motif:HMM:NF015149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HRK23506.1"
                     /translation="MRVLKPLLAASILALSAAAGQAVERSAADPGIDWSEVTGSIGLQ
                     AGPFFQFTPVAPSDGEAITSLQRFSVDSRQLRFEGEMGRRDIPVYLRKEQTADGARLK
                     LRFINSVAVMPEASRLTISINDTPVAEIALDAAGEAGNVDVAVPSGLLGAGYNAVTFA
                     VQQRHRVDCTIEAANELWTQIDASVSGLQVNAVSDAIGKFEDLNSLPVGEDGAMPITV
                     VLPQGADNVLIDQSFRAAQALAIRIGTNLPRIQFARESTQNTGILLYVGTISDLRSRG
                     VEANPLGDQAFHIAGKANGQVRVAISGATAADVNQNIFRMLVERPNKADIGTPAGLRA
                     RAAQRGIMIAPETEISLADVGLASEEFSGRVYRASFNIQMPPDFYPADNGKATMFVNA
                     EYVAGLSPTNEALVRVNGKVAGATRLARTGGDSLTRRPIEVTMKSLTAGFNTLTLEIR
                     SATDEDKDCNPLDLMQPPKRLSVDSATTFTVPKISRIFHLPNLSATAGTGFPFNETKQ
                     PLTLFMPKPDMALLGAAATLVTRVAIASGRPYETRLSSRAPDQNTDSALIVGSIRDLP
                     ISTIDYFGLKKDMVPASWQKHFGTQTAPAKTAPAKPAANAPAKGKTNAPAAKDAAAAE
                     AKPGAEAPAKAQVPLASSSIFGAPLPAPQPGTAASASLLAPAEPPPEAEEEKKDIRVR
                     WGETLDDRSSLDKARQGLSSFLQRNIGYTADQLSFLNGDRSAITATRNTRLILAQQQA
                     MAGGAATWLLLTGPDSGTIQRETTRFVAQTSWGQVQGRMVAFDPSEAELVAWPEGRHY
                     HYDMRDWSFSNITLVAAGWLSNHIQYYVAVILILCGVFGLFTRKLLNRVGAQP"
     gene            21464..22552
                     /locus_tag="PLQ11_00995"
     CDS             21464..22552
                     /locus_tag="PLQ11_00995"
                     /inference="COORDINATES: protein motif:HMM:NF013438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HRK23507.1"
                     /translation="MISLLRLVAAAVVSLGLSLPLGAEQTPFKTLRAEPDAWPAGTWS
                     AYKEKFIRAEGRVVDDGNGNISHSEGQGYAMLLAVLADDRATFDSVWAWTRKELMKRP
                     DGLASWKWVPDATPNITDPNNASDGDLLIAWALAEAADRWNHADYNREAARLAEALDK
                     ATGTRSPAGRIFLPGVNGFSAKEREDGPVVNPSYWVFPAMERLKKVALGVDWDGHRAS
                     GYTLLRTARFGAASLPTDWVSIKDKPRPADGFPPRFSYNAVRVPLYLAWTERPNKEAL
                     RPFVSYGDQNNQKMFEYDVTTSKSAEEMGGQGYLAVFALAKCAFDDTPLPPSLRSVSL
                     DQYYPTTLHMLALAAARLRFSKCWSGPR"
     gene            22531..31122
                     /locus_tag="PLQ11_01000"
     CDS             22531..31122
                     /locus_tag="PLQ11_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23508.1"
                     /translation="MLVRASLISAFLLSLIAEAGAAEIGAKASGEQGKSASPAEAATP
                     RATTAEDLFSQPMPQGMKLPFPASKPAGEAEAAAPAAAKPATSAVIRSSRQKVDESGL
                     RYFASQNQQKRVEKEIQRLKALYPDWTPPDDLFLGPPGGPDEQPFWDLFAVDKLEELR
                     ALIAERMKVEPKWKPSPALVYKLERKESRNKLVAAYEAKQWQKVIEVAASDPKIMVCA
                     DIDVPWRVAEAFSQIGQKGQTFEVYRFLLTNCRDRQTRLTTLKKSLAYFEPRQIDVLV
                     AMGARYEDGSQEFDEVKADLLRIRIGKAAEAKLNEGKPAAALPVEAKVVEAKVVEAKP
                     AQPSTAEVKPAATKAADVRAAAPKPADAKPAETKPADVLTLADLRFFEGYTRKHQNSA
                     DAALLGWYLLAHKQPAPAADMFRNALDWPAQLREDGTVMEPPAKMAEGYILALKEQEK
                     YDDAEEIAFKWSTSNDTLRLLFVEIAGEALLKAPVERTFAVDRLERFANAASRSRSAF
                     GTQALAWYHFNRQQWADAAPWFRMSLDWSDKGKEDIATVTGLVVALRSSDKFDEAEEL
                     AYAWRERDPAMRTLFIDIFAESLVRQKAPQTYPAERIARYSAEIERDRSAVGAQGLAW
                     YHHDRAQWGDAARWFRTAMDWTPGGKGTEKLAEGLAQSLRQDKQFEKAEDVAFDWHEV
                     SASLKQLFVEISAENLVTMPAERTYPMHRLNRFASHVTQIKSSPGAEAIAWYLYNRKS
                     YTEGVRWFRTALDYKPEKARELKLVEGCILSMRYLGQIVEAENYAFDWHSRSDDLRKV
                     YFEIAAEAISTLKPPATYPQAQFDRFVALTREVKSSFGAQAIGWYFHQRGEANTAAEW
                     FNTSLEWGGAITDAKTAQGYLLALISLGRMEEADKLAFEWHEKSDEFRTLYIDVFGGA
                     ILRTMPPQTFPEGMLKRYEYITAFGRSVFGSQAIAWYYYDRQDWPSSSRWFQASLDWS
                     GEAFHDQKTVEGFAQSIRYLARREEAEDILYAWRDKNPLLRKLYIELFSEALVNTSNP
                     VFFSDERLERYAEVVLNDRDFFGAQGLAWYRHDRRQWPDAVRWFKYAREWSKDAAGDA
                     KMAEGLAFSLRNLDLYDESEMVSVEWKDKRPAMRKLYSETVGEFLVRMKPEFSYPDDR
                     MERFLDTVTADANNIGAQSAAWYYYARAMYDPAANWFLQSLKWGPDFVRDPKTAEGYI
                     TSLRLGGRLAEAELAAFDWFDKSPALRQLYFEIAGQLLVEIKPPQTFPEERLARFAEM
                     ATEARSVTGAIALAWYHQTRSDHKASVEWFERALEWGGDTPDVAAVEGYVVALIGLDR
                     FEDAENLVFKWRDRSDKIAQLYIDVFGGKLLSYPQNKHIDPARLDRYAMLAGFERSIY
                     GAQALAWYYYDRQTWPEAARWFEANLTWSGTGNHDQKSVEGFAQSLRYIGRREEAEEI
                     LYAWRDRNPTLRKLYIEIMSEGLVSSSNPVFFSDERLERYAEVILKDRDFFGAQGLAW
                     YRHDRRQWPDAVRWFKYAREWSADGKGDAKMAEGLAFSLRNLDLYDESEFVSVEWKDR
                     SPAMRKLYSETVGEYLVRMKPDYTYPDDRMARFLAVTNEDANNIGAQSAGWYYYAREM
                     YDPAANWFLASLNWGPDVVRDPKTAEGYVTSLRLGGRLVEAEAAAFTLRDRSPALAAM
                     YFDIAGQVLTTLKPPESFPVERLARFAELSSESRSVAGAQALAWYHQAREEWSEAAYW
                     FQKSIEWNDKTDDPKSHEGLLVALVGLGRYEEAENLIHTWRTTNPAMAAMYMDVFGGA
                     LLRFPRTEQIDIARLDRFGVLAGLEKSVFGAQALAWYHYERENWADAVRWFQSSLDWT
                     QAGKHDVKSIEGFVQALRHVGRRDEAEELLFQWRDANSGMRKLYLETFGEALINQSTP
                     IIFPDDRIDRYAHIALADRSAFGAQALAWYRYNRRSWADAARWFKNARAWSPDGKGDA
                     KLAEGLAFALRNMDSYDESEAVSWEWRERSPAVRKLYTETVAEFIVRLKPNILYPVDR
                     LSRFQTIVTNDKSSIGAQSIAWYFHARERCGEAVPWFRNALDWGPDFVRDPKTAEGLA
                     SCLRLSGSIDEAETFAYSWRDRSDSLREMYLEYAAFALSRVQPPATYPVDRMARFVAL
                     TNTLKSHAGAQAIAWYRFSRKEWDEAIVWFKQALDWQGDTKDVRTVEGYTLALRNAGR
                     FDEAETLALAWRDRSDSLGQQYLEMLAETMARMPATETLSPDRLQRFSQLVTERQWTA
                     GAQALGWYMVNRQKLGEGETWFKNALAWSADGQKDPGLIEGYALAQKLQNKYGEADTA
                     IVELVDKSDKARELYQLNWMEWLVKTKPPATVPPERLKKFVDLATFTKHAASARSLGW
                     YFYDRKDWAQAHVWFKNATDWEADKTNAQGAEGYILTLRQMGKDEEAEALAYDWRDRS
                     EAIRALYIDSIPAMLAKLGETGTFPTERLNRFAAQVTAAKSAYGAQSLGWYRFQRKDY
                     AQAVGWFKSALDWAGDAKDPKLAEGYAMALRASGRVDDAETIAYDWRERADGLRNLYV
                     EIFAEALTKNNPPPAFAPERLARYAAIVSADKSATGAQALGWYSYNIKQLRPARAWFE
                     KAMQWGPSEGSALGLALAAQAMNDKAGFATIITNYRGTYPKLGDLLNPQTATATQTLA
                     VTNAPRMVLSTAPSVRYVEEVETVTAPSSRRVVTRDSRPPPPTETTRKGANCHSGLDP
                     VNLGWCLLNNDRPQEAAQAFERGRNGPKAADAAYGESLARLRSGETDEAIRSSMSAPM
                     SQKQRTDVTQQILAQRIIASYRADRYYDTLRYLDERGAVAPETRDLAMLRGWSLYKIG
                     RTDAARQIFTELDRQLSTKDTQNALSVLMEIRGKY"
     gene            31142..32161
                     /gene="bcsN"
                     /locus_tag="PLQ11_01005"
     CDS             31142..32161
                     /gene="bcsN"
                     /locus_tag="PLQ11_01005"
                     /inference="COORDINATES: protein motif:HMM:NF028348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsN"
                     /protein_id="HRK23509.1"
                     /translation="MLRLVTVGMLAVGLSGCGMVFDYSDPVGDPKPFDTTLGVSIPPS
                     QAMAMLPPGAGQVVSVIERRRGNAIQHDITLAGAPPNFGENQINVTAFRYVEESAENP
                     KTDMLKIERPDEATIYEEMQERIPGGALPTSNAVPRNAMGNFGYAFGRRNGANCLYAW
                     QWLESGQARPFSPFAGKTPAPVSVRVRLCRPGVTEEVLVDFVRQMFVQPRSDDAYRVS
                     ASRGPAPMAGYGAGGYGRPGMGLAATSGAYGAGYGYGNPYGGGYGDGYGPRYEAPRWQ
                     ATGTTREVAAAPVRRKKIVRRIVRRVVPQETISYSATPVTVPSSRPATMVVSPGGYSA
                     VPMPQ"
BASE COUNT         5969 a        10780 c         9729 g         5725 t
ORIGIN      
        1 ggttgaaagg cgtggatggt cgggacaagc ccgggcatga cgacgttgtg gtctgtttcc
       61 accgcaacac agcgtgatct agaaaccgac gatttcaatc gagagcatca cgccctttcg
      121 ccgtcattgc gaggatggct tctgcccgac gcggcaatcc agtatccgac ccgtttcctg
      181 gattgcttcg ctgacgctcg caatgacggc acgttgcaga cgattccatc cagaatcggt
      241 tgtgatcact cctccgctgc gccatccagc ggccgatagt tcagcaccgg tccgagccag
      301 cgctcgacct cggtaactgc catgcccttg cgctgcgcat aatcttcgac ctgatcgcgc
      361 tcgatcttgg cgacgccgaa gtaataggca tcgggatgcg agaaatagag ccccgacact
      421 gaggctcccg gccacattgc atagctgtcg gtgagcttga cgccgatccg ccgctcggcg
      481 ccgagcagtg agaacagcgt tgccttttcg gtatggtcgg gctgggcagg ataacccggc
      541 gccgggcgga tgccgcgata gtcttcgagc agcagcccgt ccttggtcac ggtctcatcc
      601 gcagcatagc cccagaattc gcgccggacg cgctcatgca tgcgctctgc gaaggcttcc
      661 gccagtcggt cggccagcgc cttggccatg atcgaggaat agtcatcgtt gcggcccttg
      721 agccgttcgg caatgcgctc ctcgccaaac ccggtcgaga ccgcaaagcc gccgatccag
      781 tcggccttta cgccttccgg cgcgatgaaa tcggcgagcg cgagattggg cttgccatcg
      841 cgacgggtca gctgctggcg cagagtgtgg agcgttgcca gcgtgtcccg gcggctctcg
      901 ccggtataga ggcggatgtc gtcgccgacg cggttggccg gccagaaacc gatcaccgcg
      961 cgcgcctgga accagcgctc ctcgacgatg cgcgtcagca tcgcctgcgc atcctcgtaa
     1021 agctgccgcg ccgccgcgcc ctgcttctcg tcctccagca ccgccgggaa ccgccccttc
     1081 atctcccagg tctggaagaa cggagtccag tcgatatagg gcacgagttc agcaagaccg
     1141 taggtcttga acacacgcgt tccggtgaac gtcggcttcg gcggggtgta attggcccag
     1201 tcggcgttaa aggcattggc acgggcgcgc gccagcggca ggcgctgctt ttcggcctcg
     1261 gcacgggcat gcgcctcggc gaccttgctg tattcggctc tgagtgcctg cttgtatccg
     1321 gcgccgctgt cggtgttgag cagcgaggaa accacgccga ccgcgcggct ggcatcaacc
     1381 acatagaccg cctggcccgc ctgataggcc ggcgcgatct tcaccgccgt atgcacgcgc
     1441 gaggtcgtcg caccaccgat catcagtggc aggtcgaacc cctgccgctc catctcgctg
     1501 gccatatgca ccatttcgtc gagcgagggc gtgatcagcc cggacagacc gatgacatcg
     1561 accctttcct tgcgtgcggc ctcaagaatg cgctcggccg gcaccatcac gccgaggtcg
     1621 atcacctcgt aattgttgca ctgcaagacg acgccgacga tgttcttgcc gatgtcgtgc
     1681 acgtcgccct tgacggtcgc catcagcacc ttgcccgccg aggcgcgctg atcagcgccg
     1741 gtgcgacgct tttcctcttc catgaacggc atcagatagg cgacggcctg cttcatcacg
     1801 cgcgctgatt tcaccacctg cggcaggaac atcttgccgg cgccgaacag gtcgccgacg
     1861 acattcatgc ccgccatcag cgggccttcg atcacatgca gcgggcgggc ggcagcactg
     1921 cgggcttcct ctgtatcggc ctcgatgaac tcggtgatgc cgttgaccag cgcatgttcc
     1981 agccgctttt cgaccggcag cgcccgccag gcgagatccg ccatcttgac ctcggccttg
     2041 ccatcgccct tgaagcgcgg cgcggcctcc agcagccgct cggtcgcatc ggcgcggcga
     2101 ttgagcacca catcctcgca cagatcgcgc agctccgccg gcagcgcgtc atagacggcc
     2161 agctggccgg cattgacgat acccatgtcc atgccgacgc ggatggcgtg gtagagaaac
     2221 accgcatgca tcgcctcgcg caccggttca ttgccacgga acgagaacga aaggttcgat
     2281 acaccgcccg agacatgcgc atgcggcaga ttctggcgga tccagcgcgt cgcctcgatg
     2341 aaagcgactc catagccgtt gtgttcctcg atcccggtcg ccaccgcgaa cacgttcgga
     2401 tcgaagatga tgtcctcggg cggaaagccg acctcttcag tcaggatttt gtaagcacgc
     2461 tgacaaatct cgatcttgcg ctcgtagctg tcggcctggc cctgctcgtc gaaggccatc
     2521 accaccacgg cagcgccata ggcgcggcac agcttcgcat ggtcgatgaa cgcctcgacg
     2581 ccctccttca tcgagatcga attgacgatc ggcttgccct gcacacattt gaggccggcc
     2641 tcgatgacat tccactttga actgtcgatc attaccggca cgcgggcgat atccggctcg
     2701 gcggcgacga ggttgaggaa ggtcaccatc gccgcttcgc tgtcgagcag gccctcgtcc
     2761 atgttgacgt cgatcacctg cgccccgttc gccacctgat cgcgggcgac atccagtgct
     2821 tcggggtatt tgccgtcctt gatcagcttg cggaaacgcg ccgaaccggt gacattggtg
     2881 cgctcgccga cattgacgaa cggaatgtcc ggcgtcagcg tgaacggttc cagccccgac
     2941 agccgcagcg ccggcgcgat ttccggcacg gcgcgcggcg ccagcccggc cacggcattg
     3001 gcgatggccg cgatatgcgc cggcgtcgag ccgcagcagc cgccgacgat attgacaagg
     3061 cccgctttgg cgaagtcggc gaccagcgcc gccatcgcct ccgggctttc gtcatagaga
     3121 ccgaattcgt tgggcagtcc tgcgttggga taggcgcaga ccagcgtatc ggcgaccttg
     3181 gcaatctccg cgatatgccc gcgcatctcg cgcgcaccga gcgcgcagtt aagcccgaac
     3241 gacaacggcc gcgcatgccg cagcgagtgc cagaaggccg tcggcgtctg ccccgacagg
     3301 gtgcgaccgg aaaggtcggt gatcgtgccg gagatcatca ccggcagccg ccggccggtg
     3361 gcggcgaagg cttgctcggc cgcgaaaatc gctgccttgg cgttcagcgt gtcaaacacc
     3421 gtctcgatca gaatgagatc gctgccacct ttgattagcg cggtgatctg ctgcgcatag
     3481 gcgatcctca ggtcatcgaa cgtaaccgcc cggaagcccg gattgttcac atccggcgag
     3541 atcgacgcgg tgcggttggt cggccccacc gcaccggcca cgaaccgccg ccggccatcg
     3601 tgcttttctg ccagcaacgc cgcctcgcgc gccagtcgcg cgccatggaa gttcagctcg
     3661 ctgacgattc cttcgagatg gtaatcggcc tgcgcgatcg tcgtgccgga aaacgtattg
     3721 gtttcaacga tatccgcccc ggccagaaag taatcgagat gcaccttccg caccgctgcc
     3781 ggctgggtga ggatcaggat atcgttgttg cccttgagcg gatgcgaatg atccttgaac
     3841 agggagccgc gaaaatcctc ttcggtcagc ttgagctgct ggatctcggt gcccatcgcc
     3901 ccatcgagca ccagaatgcg ctgccgggcg gcttcattca gcgcgcggcg aacgtcgtcg
     3961 ccgtcgacat gggtcaccgg agattgtgtc atcgttgatc cttcagcatt gtccggcttt
     4021 gctcaggcgg cggacgtggc ccgcaacccc agcatgtggc agatggcaaa caccaggtcg
     4081 gcccggttca tcgtgtagaa atggaagtcg ctgacaccgc ggtcgagcag atcgtagacc
     4141 tgctccactg cgacagccgc agccaccagc ttgcgcgtct cggcgtcgcg gtcgagcccc
     4201 tcgaaccgct gtgccagcca gtccggaacg ctggcgccgg cccgcgtggc aaagccggcg
     4261 gcctgcttga agctctgcac cggcaggatt cccggcacga tcggaatgct gattccgcgt
     4321 gcccgcacgc gatcgagata gcggaaatag caatcgttat cgaaaaagaa ctgtgtgacc
     4381 gcccgatccg cgccggcgtc gatcttggcc ttcagaatgt cgagatcctc atcaagcgag
     4441 gcgctttcgg gatgcttttc cggataggcc gaaaccgtaa catcgaaatc gccgatcgcc
     4501 ttgatgccgg ccacaagctc cgcgcttgac cggtagccgt ccggatgcgg ccggaacgca
     4561 tggccgacgc cctcgacagg atcgccgcgc agcgccacga tatgccgcac gccgaccgcg
     4621 tgataggcgc ggatcacctc ttcgacctcc gccttgctcg ccgccacgca ggtcaggtgt
     4681 gcggccggcc tcagccgcgt ctcctcgatg atgcgccgga ccgtcgcatg ggtgcgctcc
     4741 cgggtcgagc ctccggcccc gtaggtcacc gacacaaagc tcggatcgag cggcgccagc
     4801 cgctggatcg acgcccacag cgtcgcttcc atttcatcgt tcttgggcgg gaagaactcg
     4861 aacgacaccc gcggcggctg cgtggacgga gagcgcgagt ggcgcagaag cggggcaatc
     4921 atcaggcaac ctcgacggac tgtttggaaa atggcgcatc ggtctgaata cggctgtcct
     4981 tgcccagcca cagcatgacg gtcaggacgt caccctcgcc cggcagcggc ttcagcgcgc
     5041 gcacccggat cgcctccagt cccgcatcgc gcaggacgct ggcaatttcc tctggtgcga
     5101 acccgagtcg gcggtgattg aactggctgc gcagccgttc atcggcatgc ggagcaaggt
     5161 cggcgatcag cagccggccg cccggcgcga gcaaacgggc cgcttcgcgc acggcgcgca
     5221 ccggatcatc gaggaagtgc agcacctggt ggatgacgat caggttgaaa ccaccctcgc
     5281 ccggcgccag tgcataggca tcgccctgcc ggagctggac attgccaatc ccggcccgtt
     5341 cgagattggc ccgcgccacg gccagcatcg ccggcgaaac gtcgaggccg acggcacgcc
     5401 gcgcacgcgg ggcaagaatt tccagcatgc gtccggtgcc cgtgccgata tcgagcatcg
     5461 cctcgatccg gatatcgccc aggatgtcga gaatcgcggc ctcgacacgg ctttcgggga
     5521 catgcatggc gcggatgcgg ccccagtcct ccgcatgctg cgcaaaatac tgggccgcct
     5581 gcctggcccg ttccgaccgc accgccgcca ggcgccgccg gtcctcggcg acagtcacgt
     5641 cgccggggtc gatccagcgc aggatttcac cagccagcat tgccgcgcta gccgcatcgg
     5701 aacggcggaa aaacgcccag gcgccttcct tgtgccgctc caccagcccc gcttcaacca
     5761 gcagcttgag gtggcgcgac acacgcggct gcgactggcc aaggatggcc atcagttcgg
     5821 taaccgtcag ctccgcctgc gaaaccagcg ccagaatgcg caggcgcgtt ggttcaccgg
     5881 ccgcttccag cgcagaaatc gcagcgggaa aaggtaggga ggcaggcaag catcgctcct
     5941 ggcaaatgat ataaacatat ctttatatcg tttacttatt gcgctcaagg tattttgcaa
     6001 tccatgcttc ctaccgttgt ggttgagcgc acggcaaggg gccatcatcc gatcatgtga
     6061 tgcagtctga cagcgatgcg ccgatagtga tgacacttgt cgcagagcaa gaggaggaag
     6121 ccccatgcga tctttgaaga tactggctgt tttgtcgttc ttgatgatag gaacggcaca
     6181 ggcagcgttc attggcacct tcgagttggt cgacaacccc ggcggcaaag atggcaagtt
     6241 gaagaagctg aaaaatgact ttgccttcaa ggacaagagc ggcgttggct gggaagctgc
     6301 ggcgggcctt gagacggatg gcgcgtctat ccccttctgg gcgcagcctg tgatcggtca
     6361 accgtatgac ggcagttaca tcaaggctgc agttcttcac gatcattact gcgatcgaaa
     6421 ggtgcgggat tggaagaaaa cacaccgcat gttttatgac gccctcatcg aacagggggt
     6481 tcccaagatc aaggcgggcg tcatgtatgg cgccgtcatg atcggtggcc ccacctggac
     6541 cgaggtcaat gttggcaaga agtgcaaact caccgatgtc tgcattcgga cagttggcgg
     6601 cgacagagca aggatggtct ggaaccagcg gcctgccgtc tatgaccaaa tcggcgatct
     6661 ggctgaacgc ctgaggaaaa tcgagcagcg cttgaacgca gccggcgatt tcagcgcaga
     6721 ggatgtcgaa gccatcgctg tcgacttggc accggaaaac tcgaatatcc ggcagcccgc
     6781 ccatgccgaa ctggaggaaa tcagcctgcc aaccaagtag ggttcaccgg aacgggggct
     6841 cctcgaagct gcgcaatttc cgcgtatgca gctggtccag cttgccgcgc aacagatcga
     6901 cggccttgag cccgatctgc agatgctggc cgacggcccg ttcgtagaag gcgctggccg
     6961 caccgggcag cttgatctcg ccatgcaagg gcttgtcgga gacgcagagc agcgttccgt
     7021 acgggacccg cagccgatac ccgtttgccg cgatcgtgcc cgattccata tccacggcga
     7081 tggcgcgcga cagattgatg cgatgccgtt cctggctcca gcgcaattcc cagttgcgat
     7141 catcattggt gacgatcgtg ccggtgcgca ggcgccgttt cagcccctcg ccccgctcgc
     7201 ccgtcacgat ggccgccgcc tcctgcaagg cgacctgcac ttcggccagc gccggaattg
     7261 gaatctccgg aggcaccagc gcatcgagaa tgcggtcctg cctcagatag gcatgggcaa
     7321 gcacataatc cccgatctcc tggctctgcc tcagcccgcc acaatggccg atcatcagga
     7381 acacatgcgg cctgagcacg gcgagatgat cggtgatgtt cttggcgttc gatggcccga
     7441 cgccgatgtt gaccagcgtg atcccgccgc cctcgcccgc cagatgatag gccggcatct
     7501 ggaacttcga ccaggcggag atatcaatga ggctgtcgcc cgcatcgccg gtgcccggct
     7561 ccttcacctc gacattgccc ggcagcacca ttttgctgaa tcgcgttgcg ccacccagct
     7621 gggccatgcc ccagcggacg aactgttcga catagcgatg gtagttggtg agcaggatat
     7681 agggctgcac atgccgccag tcagagccgg tatagtgcac cagccgcttg agcgaataat
     7741 cgacgcgcgc cgcatcgaac agggccaacg gccagggctc gttttcgccc acctgatagg
     7801 tgccgtcggc gacctcgtcg cccactgccg aaagccgcgg caccgggaaa aaccgcgcca
     7861 gttccgccgg gcctatgccg gcgcggccca gttcgtcacc ggcctgaacg acatagggat
     7921 aaggcatttc ctggcgcgac aggccgacct gcatgcgcgc gccatattcg gcgaccagcg
     7981 gctgcaattg ttcgagcaga tagggcgcaa acgcatccgg ctgggttatc gtggtcgaat
     8041 acacgcccac ccgctggaac ttggcatagg cccggcgtgt cgaatgcggc acgccatcgg
     8101 gttcgtaggt cagcctgagt tcgggatagc ggaatgccgc ccgctcggct gcggagggcg
     8161 ggcggcgcgt ctccagataa cgacccagcg cctgtctgag ggcaccggtt gcctggccgt
     8221 gcaacccgat cagcgcgtca acggcctcgc ccggtgacgc cacctcgatg aacccgcctg
     8281 atttcatcgt cccacccgat tcgagcctgc atccttcata gcaacacgct gccggcctgt
     8341 cccggcccga tcggcggcgg cgcaatgcct ctgttcatct cgaccatgat agcgctcgac
     8401 cctgcccgaa ccatcgacta tcaccgccga atgctagggg agttcaggta aatggcaggc
     8461 ccgaaggttc tttcctccgt tgcagatctg cgcgccgccg tggccggctg gcgcagggcc
     8521 ggcgagaccg ttgcgctggt gcccaccatg ggcgcgctgc atgccggcca tgtctcgctg
     8581 gcccggatgg cccaggggaa ggcccggcgg gtcatcgtct ccatttttgt caacccgacc
     8641 cagtttgcgc cgacagagga cttttccaaa tatccccgca ccttcgccgc cgattgcgcc
     8701 aaactggccg aagccggggt tgatgccgtc tatgccccca ccccggaggt gatctatccg
     8761 caaggtttcg tcaccaccgt ttcgcttgca ggcccggcaa cggccgggct cgaggacaag
     8821 ttccgcccca cgcattttgc aggcgtcgcc acggttgtct ccaagctgca tatccaggcc
     8881 atgccggata tcgccatcta cggcgagaag gatttccagc agctggcggt gatcacccgc
     8941 atgaaccgcg acctcgacct gccgatcacc gtcctcggcg gccccacggt gcgcgaggcc
     9001 gatggtctgg ccctctcgtc gcgcaatgtc tatctcagct ctgccgatcg caaggttgcc
     9061 ccggtcctgc atgcaaccat gcaggactgc gcccggcggc tgcgtagcgg cgctgatctg
     9121 gaaacggttc tggccgccgg ccgcgcggcg atcaccagcg ccggcttcgc gctcgattat
     9181 ctggaactgc gcgatgccgc ttcgctcggc ccggcggacc ccggtgccgg gcacccgctg
     9241 cgcctgctgg tggcggcgag gatcggcgcc acccggctga tcgacaacat cgcggtgtga
     9301 tgccaacggc gctcaggagt gaccgatagg tcatgatgaa ggggcgttgg cttaggagcg
     9361 aatgcgacgc cgagcagggc cggaggcccg ttgcgaggga ccggcccgag gttgaccgtc
     9421 aggtcaatcc cgagggccac tggcagaatg ccttgtcgtc ctgcggcggc gggtctatcc
     9481 ctcggcgcca gcgacgaggc cctcatgaca aatgttcccc ctgaacttgc caccctgcgc
     9541 gactttctgc gctatgccgt cagccgcttc cgcgccgcca aactcgcctt cggccatggc
     9601 accgccaatg cccatgacga ggcggcgttt ctgctgcttg aggcgctgca tctgccgatc
     9661 gaccggctcg atccctatct cgatgcgcgg ctgaccggcg acgagcgcca gcgcatcgcc
     9721 gatctgatcg aggcgcgcat caccacccgc aaaccggcct gttacctgct caacaaggcc
     9781 tatatccagg gcatcccgtt ctatgtcgac gagcgggtga tcgtgccgcg ctcctatatc
     9841 ggcgagatgc tgatgggcgg actgttcgag gatggcccgc tggcggacga acgcgacccc
     9901 gccgaggtcg gccgcgtgct cgatctctgc accggttcgg gctgtctcgc cattctcgcc
     9961 gcgcatgtgt ttccgaacgc gaccatcgac gccgccgacc tgtcgcgcga tgcgcttaag
    10021 gtcgcccgcc gcaatgtcga cgagcatggg ctggaggatc gcgtcgcgct gatcgaaagt
    10081 gatctgttca gcgcgctgaa aggccgccgc tacgatctga tcctcaccaa tccgccctat
    10141 gtgacggcgg cagaggtcgc cgccttcccg cccgaatacg cggccgaacc gcagatggcc
    10201 cacctcggcg gcgccgatgg catggatctg gtgcgcacca tcctcaagga tgcgaaaaaa
    10261 cacctcaacc ccggcggcgg cctgctctgc gaaatcggcc tcgggcgcga gatcatcgaa
    10321 agtgagtatg ctgacattcc cttcctctgg ctcgacagcg aggagagcga gggggaggtg
    10381 ttttggctac agaaagagga acttccctaa agaatttcat ttcaaagcgg gctgtttgat
    10441 ctggctatct gccaatcatc gtaggtcaaa ttcttttcgc caacgacttt ccattctgta
    10501 cgaccgtttg aattacggtc tagcaccact gctgccgccg ccgaggggct cctgaacggg
    10561 aagtcctcaa caaaaacata tcgtttgcca gcaggatcag gaaccatctt cccttgctcg
    10621 atgagttcct tccgcagttc tccataagag ctgttatgct tgtagccagc atccttaaga
    10681 gcttcgctgc ccttacggac tatgaattct ccatcaattt ccaatgcaaa agccttgatg
    10741 ccacttttgt gtcgaatttc aaattgagac ctgtcgtcgc cgagagatga atccgttgcc
    10801 agcgtccgcg tcgccttggg tttaagaaat tcgaagccaa ccactggcaa cacaactttc
    10861 agattttcaa gaaatgcttc catattggct ctgtctgctt ccggcaggcg cttccgatcc
    10921 gcagatggtg cgttgccatt gtccaacgtc acccggccag ccatgacggc catttcgata
    10981 agtcgcgcct ccaaataacg aacatgccct ttcgttaggg cttcatcgct cgtagtaacg
    11041 accgcaaccg tctcccaaaa ggcgcgggtc ttggcgcttt ggccaacacg ctcccgaacg
    11101 ctgtcacctt cgccaatata agcgcgcatc gacaacggat catcgggatc cgggccaaac
    11161 aagacataaa tgcccgtccg atccgcttcc ggacgcgcca gcaacttcgc aaaagtggat
    11221 tgggtcgata caagaataga gcctgtccac ccatgaatcg aagcaatgat cagaccattg
    11281 ggaacgccat caaccagaaa caattggatg gtgcgtccgg tgcctgtctg atcatttgac
    11341 atctctcacc tcgtgaactg ccggtaattg atccgcttcg gcaacactga atccggcccc
    11401 agccgccgct tcttgtcctc ttcgtaatcg gcaaagttgc cctcgaacca ctcgacatgg
    11461 ctgtcgccct cgaaggcgag gatgtgcgtg gcgatgcggt cgaggaacca gcgatcatgg
    11521 ctgatgatga ccgcgcagcc ggcgtaatcc tccagcgcct cttccagcgc gcgcaaggta
    11581 tcgacgtcga gatcgttggt cggctcgtcg agcagcagga cgttggcgcc cttcttcagc
    11641 atcttggcaa gatgcacgcg gttgcgctcg ccgcctgaca gcatgccgac cttcttctgc
    11701 tggtcgccgc ccttgaaggc gaaggcgccg caataagccc gcgcgttcat ttccttcttg
    11761 ccgaggtaga tcacctcgtt gccgccggag atttcctcat agaccgtctt cttgtcgtcg
    11821 agactgtcgc ggctctggtc gacatagcca agctgcaccg tatcgccgat cacgatcttg
    11881 ccgccgtccg gctgttcggt gccggagatc atgcggaaca gcgtcgtctt gccggcgccg
    11941 ttcggcccga taacgccgac gatgccgccc ggcggcagct tgaacgacag atcgtcgatc
    12001 agcagcttgt cctggaatcc cttggaaagc ccttcgaaat caatgacatt gccgccaagc
    12061 cgctcggcaa tcgggatgat gatctgtgcg gtctgcgcgg tcttttcctc agcacgctga
    12121 acaagttcgt cataacgctg aatacgtgcc ttgttcttgg cctgccgggc tttcggcgag
    12181 gccgccaccc attccgactc gcgcgccagc gtgcgctggc gggcggcctc ctcgcggccc
    12241 tcctgctcca gccgtttctg cttctgcgtc agccacgagg aatagttgcc ctcgtaaggg
    12301 atgccctgcc cgcgatcgag ttcgagaatc cagcccgtca cattgtcgag gaagtagcgg
    12361 tcatgggtga cgatcaggat cgcgccggga tactcgcgca ggtggttttc cagccattgc
    12421 gtggtctcgg cgtcgagatg gttggtcggc tcgtcgagca gcagcagatc gggcttttcg
    12481 agcagcagct tgcacagcgc gacgcggcgt ttctcaccgc ccgagagctt gtcgacttgc
    12541 cagtcatccg gcgggcagcg cagcgcatcc atcgcctgat cgatgcggga atcgagatcc
    12601 cacaggttct cggcgtcgat cacatcctgg atcttggcgg ccttttcggc attctcgtcc
    12661 gaataatcgg ccatcagcgc gttgtattca tcgatcagcg cctgctcccg cgccaccgcg
    12721 agcatcacgt tctcgcgcac ggtcctggtc tcgtcgagcc tgggctcctg cggcaggtag
    12781 ccgacgcgcg cgccctcggc cacccagcct tcgccggtcc attccttgtc gatcccggcc
    12841 atgatcttga gcagcgtcga tttgcccgaa ccgttgacgc ccagcacgcc gatcttggca
    12901 tcgggataga acgacaagtg gatgttgttc agcacctgct tgccgcctgg ataggtcttg
    12961 gacaggccgc gcatatggta gatgaactga cgggacatcg gggctctttg tgcttgagga
    13021 ttgatctgcc gccgatgtag cggggcgagc gcccccgcgc aaggcgggga taccgatccg
    13081 ccgcaggcta gatcagccgc aatgccactc ccaacaccgc gcaaagcccg agcaccggga
    13141 tcattcccag atggaggcgg aacagcgcca ccatcgccag gatggtcagc accgccgctt
    13201 gcccgtccag gctggtccat tccggccaat gcagccgcag cggtccggcg gcaacctcac
    13261 cgacgcgcgc aaacagcgtc tgcaacgcaa accacagcga caggttgagg ataaccccca
    13321 ccaccgccgc gccgatcccg gccagggcac ccttcagcgc cggattgtgc cgcacgcgtt
    13381 ccagaaaggg ggcgccgagg aaaatccaca gaaagcaggg aatgaacgtc acccaggtcg
    13441 tcatcacggc gccaagcacg ccggccagca gcggcggcag cgtcgccggc gcctgatagg
    13501 cggcaagaaa gccgacaaag ctcaacacca ggatgagcgg ccccggtgtc gtctcggcga
    13561 gcgccagacc gtcgatcatc tggccgggct tcagccagcc ataggtctcc accgccgcct
    13621 gcgccacata ggccagcacc gcataggcgc cgccgaaggt caccaccgcc atcttggaaa
    13681 agaacaccgc cagcgccgga aacacggtgc ccgcgccgaa cagcgcaatc atcgccagcg
    13741 gcagcgctgc ccataatccg ccccagatgg ccagcgtcgt cacgatgtgg tgccagcccg
    13801 gcactggcac gtcggcggca ggcaaggtcc ccgatgcaga aacgcccgcc agccggcctg
    13861 cgagcgccca gcccgcaatc ccggccgcga gcaccaccag cggaaacggc acgcggaagg
    13921 caaacagcgc cagaaacgat gccgccgcaa tggcatgggc ccagccaccg cgcagcgact
    13981 tcttcgccac gcggaccagc gcttcaagca caaccgcgag caccgccgcc ttcagcccga
    14041 aaaacagcgc cgtgaaccac gtctggcttt cgaactgggc atagagcatc gacagcgcca
    14101 ggatcacgca gaagccaggc gcgacgaaca gaacgccggc gatcagcccg ccgagaaccc
    14161 catgccgcag ccagccgata taggtcgcca gctgcatcgc ttccggcccc ggcagcaacg
    14221 tgcagaagtt gagcccgtgc aggaactggt cctccgacac ccatcgtcgt tcctcgacca
    14281 gcatcctgtg catcagcgcg atctggcccg ccgggccacc gaacgacatg aggccgatgg
    14341 tcagaaaggc gcggaatgtc tcagccagcg aagccgtcgc ggcgccctgt ctcattgcgt
    14401 cttccccgca ggccagttgt gggtttctcc cgacgcctcg acacaccagc gatagagcgc
    14461 gtcatagatc agcattccgg catcgagctg ggcaagatcg tcgctgaaaa gccgcgacag
    14521 gccgagtgaa acagccagca gccccgccgc ctgcggcgca aggtcaagcc gcgctgtatc
    14581 tgctccgcgc acgattgcgg ccaaccgttg catcggctca tgcatcaggt caaacgcgcg
    14641 gatcatggta tcgaacgtgc actccgggcc gctgtggctc cagcgcacgc cttcgacatc
    14701 gaacggaata gccccgaatc gctcagccac tgccgcgacc tcggtgtccc tgacgaagag
    14761 aaactcggcg tttctgtcga tgaagcgccg gatcagccac gggcaggcaa tccggtcgat
    14821 ctttggccgc tcgcgtgtca cccacaggct gcgcgccgcg ccccctgcca gcggattttc
    14881 tccttccggc accagcggca accccgcctt tttccaggct tcaaagcccc cctccaggac
    14941 gcgcgccgtg atcccgtcga gccgcaacat tgcggcagcg ccctcgctca gcttgagacc
    15001 acgctggcag acgacggcca ccacgcgatc ggatcgtcgt gccatgaaat ccttgaccga
    15061 ggaatgggaa caaagcactg agcccggcag tcgccgggga tctgcagcaa agtcatcgtc
    15121 gatgcgcaca tcgacgatga gtggcgcttc ggcacgcccg agactgagaa acagatcttt
    15181 ggtggataac ctggggtcaa acggcatacg cgccccttcc ctgaaaagcg agaatggacg
    15241 cgattcttgg gcataccgcc tgacggggag atcgcgaaaa cccccgatgc cgacagctga
    15301 cacagaccag aggtgcccgt caacccgctg gttagggcac atttcccgaa gtgccccctg
    15361 acgcagctcc gactgaatac ttgtgcttct gcgccgtagc gtcgccgttg gtccggtgtg
    15421 caagtcccgc agcggcaacg taaggttttt gcaacaacgg gtagctgatt tcagtcccta
    15481 ttaaccatgc cgatcgagaa cagcggcgca atggaccaaa tacaacccct gcgcgacaac
    15541 gatggtataa attcacttct gcttattctt tccaagtcat ttctgagcgg tcaattgcca
    15601 gaagcaggcc tatcgtccgt gaccgcatcc gcccaactac agccccagca gcccgcgcgc
    15661 gttctggtcg tcgcgcagga tgccgcgtcg gtggcgtcgc tgacatcgga gatgatgcgc
    15721 cgcagcgcgc tggtggcgat tgcggcgacc gtggccgagg ccggcgcggc ggttcgttcc
    15781 aatggctttg acgtcatttt cgtcgccgcc cgcgaggccg atgccgaaac cgcgctgctg
    15841 atccagttgc tgcgcggcgc ttcgcagatc gccccccgca tcctgttcct cgcccaccct
    15901 gatcacattg ccgatcatcc gatggcggtc gctgccgccg atgcgacgat ttcctggacg
    15961 cttcctgccc ttgagatcgc cgatctggcc gtgccgcgtg ccgaaccgcc gaagaagaag
    16021 ccggcgcaga tcatcaccaa gccgtcgcgc aagacccgga cggcagagat cgaagccgcc
    16081 gacgatgaga ccgcagcatc gattgatgcc gcgctgatcc ctgcggccga gaccattgcc
    16141 cgcgcagcag aagcgatcgc ccgcgccgcc gagagcatcg caagccggca gatgcaggcg
    16201 tctgagcggc cggccgagac cnnnnnnnnn nactgaccgg ttccgccgag gtcgaggaca
    16261 ttccttcccc ggccgaaccg gtcagtgctc cggtcgccga agtcagcgtc gagcagcccc
    16321 tcgtccaggc tccagcggtc ccccgcgccc cggcgccggc acctcacctt gccgccgtca
    16381 gcactccacc cgctccagcg ccggtgaccc cgccggcatt gcgcgaggca ccgaagccgc
    16441 atgtcgccac ggtggcacgc ctgccgcagc gtccggtgca tatgcccgag ctaccgccca
    16501 agccggcggt cgaggcggtg ccggagccgc agggtgaagc caggcagaac cgccgcttct
    16561 atgatgccaa ggacgcggca ccgaagcatt cgcgcgcctc gaagctgttc tttgcaaccg
    16621 gttggttcct gcttgccggc gccatcgtcg gtctgaccac ctggccgatc tcgacccaga
    16681 cccagttcac gctgggtctc gtcgtgctcg cggcaatgac ggtcatccgc aagctgcgcc
    16741 tcggcggcat ctggcggccg acgttcctgg cgctcggcat caccatgatc ctgcgctatg
    16801 tctactggcg caccacatcg acgctgccgc cgatgcacga ctgggtgaac ttcacgcccg
    16861 gtttcatgct gtatctggcg gaaatgtatt gcgtcctgat gttcttcatc tcgatgttca
    16921 tcatcgccga tccgatcgag cgcaagccgc cgccggacct gcccgatgat cgcctgccaa
    16981 cggtcgatgt ctacatcccc tcctacaacg agggtgcgga tctgctcgcc gcgacgatcg
    17041 ccgcagccac gcagatggat tatccgaagg acaagctgaa cgtctatctg ctcgacgacg
    17101 gcggcaccga ccagaaatgc aaccagtcgg acccggaaaa ggccgccgaa gcgatcgccc
    17161 gccgcgccga attgcagtcg ctctgcctgg aaatgggcat caactatatc acccgcgcgc
    17221 gtaatgagca cgccaaggcg ggcaacatga acaatgccct gcagcagacc agcgggcaga
    17281 tcatcgtcgt gttcgacgcc gaccatgcac cggcccgcca gttcctgcgt gagacggtcg
    17341 gctatttcgc gctcgaccgc cggctgttcc tcgtccagac accgcacttc ttcatcaacc
    17401 ccgacccggt cgagaagaac ctccagacct tcgatcgcat gccatccgag aacgagatgt
    17461 tctacggcac gatccagaag ggcctcgaca agtggaacgg cgccttcttc tgcggttcgg
    17521 ccgcgctgat ctcgcgtcgt gcgctggaaa gcgtcggcgg cttttcgggc atctcgatca
    17581 ccgaggattg cgagaccgcg ctcgatctgc acaagcgcgg ctggaattcg atctatgtcg
    17641 acaagccgct gatcgccggc ctgcagccgg aaagccttgt ctcgttcatt ggccagcgct
    17701 cgcgctggtg ccgcggcatg gtgcagatcc tggttctgaa gaacccgctt ctggcccgca
    17761 acatctcgtt aaagcagcgc atctgctacg tttcgagcat gttctactgg ctgttcccga
    17821 tcccgcggat gatcttcctg ctgtcgccgt tcctctacat cttcttctcg atgcagattt
    17881 tccaggcgaa cgcacaggag ttctttgcct acgtttcgat ctacatgctt acccacatgt
    17941 cgatccagaa ctacctgcat ggttcgaccc gctggccgtg gatctccgaa gcctatgaat
    18001 atgtgcagac catctatctg gtgaagggca tctggggcgt gctgctcaat ccgcgcaagc
    18061 cgaccttcaa cgtgaccgcc aagggcctga cgctcgacaa cgaccatctt tcggaactgg
    18121 cctggccgta tttcgccttc ttcggcctga tcctgctcag ccttgtggtg gcggtcagcc
    18181 gctttctggc cgagaacgag ttcaatgaac tgctgctgat cgtcggcggc tggtcgttct
    18241 acaacctgct gatcgccggt gttgcgctgg gcattgttgc cgagcgccgc gagcgccgca
    18301 agacgccgcg cctggtgatc gagcgggcgg gcgaacttca tgtcggcgag cacatcgttc
    18361 cggtcgacat ccaggattgc tcgatcggcg gcatgcgcat caagccgaag gagggccgcc
    18421 tgccggaggg ctacgggcgc ggcacaccgg cgttcattca tgtcctgcct acccggcccg
    18481 atttcgatac cgacgcgatc ccctgcacga tccagaatgc atcgaacgat ggcaagggcg
    18541 ccgtctatgg cttgcagttc cgcgagctca ggccggtcca tttccgcggt atcgccgacg
    18601 ccatgtattc ggacatggcg gtgttcgaga atttccgcat gagccgccgc aagatcaagg
    18661 gcatcaccat gggcacgttg cagttcctgt ggtggtcgat gcaccacacg gtgcgcgcct
    18721 tctattacat gatcgccctt cgcaacgtcg aagcccccaa ggtgcaggac ggcgagcaga
    18781 tggccccgac cgaatacaag ggcgcccatc ccgacttcca cgagccggcc actccggtgg
    18841 acatcaccca tccccagccc gaggcgatta ttgcccggcc ggtgatcacg gcgcactgag
    18901 gaggcggtgc gatgcgcgtc ttgaaacccc ttcttgcggc ctcgatcctg gccctgtcgg
    18961 ctgcggcagg acaggcagtc gagcgctccg ctgccgatcc gggcatcgac tggtcggagg
    19021 tcacgggctc catcggcctg caggccggcc cgttcttcca gttcactccg gttgccccga
    19081 gcgatggcga ggccatcacc tcgctgcaga ggttcagtgt cgattcccgg cagctccgct
    19141 tcgagggcga aatggggcgc cgcgacatcc cggtctatct gcgcaaggaa cagaccgctg
    19201 acggcgcgcg gctcaagctg cgcttcatca attcggtcgc ggtgatgccg gaagcctcac
    19261 ggctgacgat cagcatcaac gatacgccgg tggcggaaat cgcgctcgat gctgcgggcg
    19321 aggcgggcaa tgtcgatgtt gccgttccgt ccggattgct cggtgccggc tacaatgccg
    19381 tgaccttcgc cgtgcaacag cgccatcgtg tggattgtac catcgaggcg gccaatgaac
    19441 tgtggaccca gatcgacgca tccgtgtcgg gtcttcaggt caatgcggtc agcgacgcga
    19501 tcggcaagtt cgaggatctc aactccctgc cggtgggtga ggatggcgcc atgccgatta
    19561 cggtggtgct gccgcagggc gccgacaatg tgctgatcga ccagtcgttc cgcgccgccc
    19621 aggcgctggc aatccgcatc ggcaccaatc ttcctaggat ccagttcgcc cgcgagtcga
    19681 cgcagaacac cggcatcctg ctctatgtcg gaacgatctc cgatctgcgc tcgcggggcg
    19741 ttgaagccaa tccgctcggc gaccaggctt tccatattgc cggcaaggcc aacggccagg
    19801 tgcgcgtggc gatttccggc gccacggcag cggatgtgaa ccagaacatc ttccgcatgc
    19861 tggtcgaacg gcccaacaag gccgacatcg gcacacccgc cggtctgcgc gccagggcag
    19921 cccagcgcgg catcatgatc gcgccggaaa cggaaatctc gctggcggat gtcggacttg
    19981 cctccgagga attcagcgga cgtgtctatc gggcctcgtt caatatccag atgccgccgg
    20041 acttttatcc ggctgacaac ggcaaggcga cgatgttcgt caacgccgaa tatgttgccg
    20101 gtctctcccc caccaacgaa gcgctggtgc gggtcaatgg caaggtcgcc ggcgccaccc
    20161 gcctcgcccg gacgggtggc gattcgctga cccgccgtcc catcgaagtg acgatgaagt
    20221 cgctgaccgc cggtttcaac acgctcacgc tggaaatccg cagcgccaca gacgaggaca
    20281 aggattgcaa cccgctggac ctgatgcagc cacccaagcg cctcagcgtc gacagcgcaa
    20341 ccacattcac ggtgccgaag atctcccgca tcttccacct gcccaatctc agcgccactg
    20401 ccggcactgg atttccgttc aacgaaacca agcagccgct gacgctgttc atgcccaagc
    20461 cagacatggc attgcttggc gcggccgcca cgctggtcac ccgtgtggcg atcgccagcg
    20521 gccggcctta tgaaacgcgg ctatcgtcgc gcgctcccga ccagaacacg gattcggcgc
    20581 tgatcgtcgg ctcgatccgc gatcttccga tcagcaccat cgattatttc ggcctgaaga
    20641 aggacatggt gccggccagc tggcagaaac attttggaac gcagaccgcc cctgcaaaga
    20701 ccgccccggc caagcctgcc gccaatgctc cggccaaagg caagacaaat gcgcccgccg
    20761 ccaaggacgc agcagcagcc gaggcaaagc ccggtgcgga ggctcctgcc aaggcccagg
    20821 tgccgctggc cagttcctcg atcttcggtg cgccgctgcc cgccccgcaa cccggcaccg
    20881 ccgccagcgc cagtctgctc gcacctgccg agccgccacc ggaggcagag gaggagaaga
    20941 aggatattcg cgtgcgctgg ggcgagacgc tggacgatcg cagcagcctc gacaaggcgc
    21001 gccagggcct gtccagcttc ctgcaacgca acatcggcta tacggccgac cagctctcgt
    21061 ttctcaacgg agaccgctct gccatcacgg cgacgcgcaa caccaggctg atcctcgccc
    21121 agcagcaggc aatggctgga ggagccgcca cctggctgct gctgaccggc ccggattccg
    21181 gcaccatcca gcgtgagacc acccgctttg ttgcacagac cagctggggc caggtgcagg
    21241 gccgcatggt tgccttcgat ccgtccgaag ccgaactcgt cgcctggccg gaaggccggc
    21301 attatcacta tgacatgcgc gactggtcgt tctccaatat cacgctggtg gccgccggct
    21361 ggctgtccaa tcacatccag tattatgtgg cggtgatcct gatcttgtgc ggcgtcttcg
    21421 gcctcttcac ccgcaaactg ctcaaccggg tcggcgccca gccgtgatca gcctgctccg
    21481 tcttgtcgct gcggctgttg tcagcctcgg cctgagcctg ccgcttggcg ccgagcagac
    21541 gccgttcaag acgctgcgcg ccgaaccgga cgcctggccc gccggcacct ggtccgccta
    21601 caaggaaaag ttcatccgcg ccgagggccg ggtcgtcgac gacggcaatg gcaatatcag
    21661 ccattccgag ggccagggct atgccatgct gctggctgtt cttgccgatg accgggccac
    21721 attcgactcc gtctgggcct ggacccgcaa ggaactgatg aagcgccccg atgggctggc
    21781 ctcgtggaaa tgggtgccgg atgccacccc gaacatcacc gaccccaaca acgcctccga
    21841 cggcgacctg ttgatcgcct gggcactggc cgaggccgcc gatcgctgga atcatgccga
    21901 ctacaaccgt gaggctgccc gccttgccga agctctcgac aaggcgacag gcacacggtc
    21961 gcccgccgga cgcatctttc ttcccggcgt caatggcttt tccgccaagg agcgcgagga
    22021 cggcccggtg gtgaatccgt cctactgggt gttcccggcc atggagcgcc tgaagaaggt
    22081 ggctcttggc gtcgattggg acggccatcg cgcctcgggc tacaccctgc tgcggaccgc
    22141 gcgcttcggc gcggccagcc tgccgaccga ttgggtgtcg atcaaggaca agcccaggcc
    22201 cgccgacggc tttccgccgc gcttctccta caatgccgta agggtgccgc tgtatctggc
    22261 ctggaccgaa cggccgaaca aggaagccct gcgtcctttc gtcagctatg gcgatcaaaa
    22321 caaccagaag atgtttgaat atgacgtaac aacctccaaa tccgccgagg aaatgggggg
    22381 gcaaggctat ctggccgtat tcgcactggc aaaatgtgca tttgacgaca cgccactgcc
    22441 gccaagttta cgttctgtaa gccttgatca gtattatcct acgacgctgc atatgttggc
    22501 gttggcagcg gcgcgactca ggttcagtaa atgttggtca gggcctcgct gatttcggct
    22561 ttcctgcttt cgctcattgc ggaagcgggc gcggcggaaa ttggcgcgaa ggcatccggc
    22621 gaacagggca agtcggccag ccccgccgag gcagcgaccc cgcgcgcgac cacagccgaa
    22681 gaccttttca gccagccgat gccgcagggc atgaagctgc ccttccctgc cagcaaaccg
    22741 gcgggcgaag ccgaggctgc cgccccggca gcagccaaac ccgccacatc tgcagtcatc
    22801 cgctccagtc gccagaaggt ggacgaaagc ggcctgcgct attttgcctc gcagaaccag
    22861 cagaagcgcg tcgagaagga aatccagcgc ctgaaagcgc tctatccgga ctggacaccg
    22921 cccgatgacc tgttcctcgg cccgcccggc ggtccggacg agcagccgtt ctgggatctg
    22981 ttcgccgtcg acaagcttga ggaactgcgt gcgctgattg ccgagcgcat gaaggtcgag
    23041 ccgaagtgga aaccgtcgcc ggcattggtc tacaagctgg agcgcaagga atcgcgcaac
    23101 aagctggtcg ccgcttacga ggccaagcag tggcagaagg tgatcgaggt cgcggcctcc
    23161 gatcccaaaa tcatggtctg cgccgatatc gacgtgccct ggcgcgtcgc cgaagctttt
    23221 tcgcagatcg gccagaaggg ccagaccttt gaggtctacc gcttcctgtt gaccaactgc
    23281 cgtgaccgcc agacgcgcct gaccacgctg aagaaatcgc tcgcctattt cgaaccgcgc
    23341 cagatcgacg ttctcgtcgc aatgggcgca cgctacgagg atggcagcca ggaattcgac
    23401 gaggtcaagg ccgatctgtt gcgcatccgc atcggcaagg ccgccgaggc caagctgaac
    23461 gaaggcaaac ccgccgccgc acttcccgtc gaggcgaagg ttgtcgaagc gaaggttgtc
    23521 gaagcaaagc ccgcccagcc aagcaccgcc gaggtcaagc ctgcggccac gaaggcggcc
    23581 gacgtcaggg ctgcggcccc taagccggca gacgccaaac ctgccgaaac caaaccggcg
    23641 gatgtcctga ccctcgccga cctgcgcttc ttcgaaggct atacccgcaa gcaccagaac
    23701 tccgccgatg cggcgctgct gggctggtat cttctcgccc acaagcagcc cgcgcccgcc
    23761 gccgacatgt tccgcaatgc cctcgactgg ccggcacagc tgcgcgagga cggcaccgtg
    23821 atggagccgc cggccaagat ggccgagggc tacattctgg cgctgaagga gcaggaaaaa
    23881 tacgacgacg ccgaggaaat cgccttcaag tggtcgacat cgaacgacac gctgcggctg
    23941 ctgtttgtcg agatcgccgg cgaggcgctc ctgaaggcgc cggtcgagcg aaccttcgcg
    24001 gtggaccggc tggaacggtt tgccaatgcc gcctcccgct cccgctcggc ttttggcacg
    24061 caggcgctcg cctggtatca cttcaatcgc cagcaatggg ctgatgccgc gccctggttc
    24121 cgcatgtcgc tcgactggtc ggacaagggc aaggaggaca tcgccaccgt taccggcctg
    24181 gtggtggcgc tgcgctcctc cgacaagttc gacgaggccg aggaactcgc ctacgcctgg
    24241 cgcgagcgcg atccggcgat gcgcacgctg ttcatcgaca tttttgccga aagcctggtg
    24301 cgccagaagg cgccgcagac ctatccggcc gaacgcatcg cccgctattc cgccgagatc
    24361 gagcgcgacc gctcggcggt cggcgcgcag ggccttgcct ggtatcacca cgatcgcgcc
    24421 cagtggggcg atgccgcccg ctggttccgc accgcgatgg actggacgcc cggcggcaag
    24481 ggcaccgaaa agctggccga gggtcttgcc cagtcgctcc ggcaggacaa gcagttcgag
    24541 aaggccgagg atgtcgcttt cgactggcac gaggtttctg ccagcctgaa gcagctgttc
    24601 gtcgagattt cggctgaaaa tcttgtgacc atgccagccg aacgcaccta tccgatgcat
    24661 cggctgaacc gctttgccag ccatgtcacg cagatcaagt cgtctcccgg cgccgaggcc
    24721 atcgcctggt atctctacaa ccgcaagagc tacaccgaag gcgtgcgctg gttccgcacc
    24781 gcgctcgact acaagccgga gaaggcgcgc gaactcaagc tggtcgaagg ctgcatcctg
    24841 tcgatgcgct atcttggcca gatcgtcgag gcggaaaact atgccttcga ctggcacagc
    24901 cgatcggacg acctgcgcaa ggtctatttc gagattgccg ccgaggcgat ctcgacgctg
    24961 aaaccgcccg cgacctatcc gcaggcccag tttgaccggt tcgtggcgct cacccgcgag
    25021 gtcaagtcca gcttcggcgc ccaggccatc ggctggtatt tccaccagcg cggcgaggcg
    25081 aacaccgctg ccgagtggtt caacacctcg ctcgaatggg gtggggcgat caccgatgcc
    25141 aagaccgccc agggctatct tctggcactg atcagccttg gccgcatgga ggaggcggac
    25201 aagctcgcct tcgaatggca cgagaaatcg gacgagttcc gcacgctcta tattgacgtg
    25261 ttcggcggcg cgatcctgcg caccatgccg ccgcagacct tcccggaagg catgctcaag
    25321 cggtatgaat acatcaccgc gttcggcagg tcggtgttcg gctcgcaggc catcgcctgg
    25381 tattactacg accggcagga ctggcccagt tcctcccgct ggttccaggc cagcctcgac
    25441 tggtcgggcg aggctttcca cgaccagaag acggttgaag gcttcgccca gtcgatccgc
    25501 tatctcgccc gccgcgagga agcggaggac attctctacg cctggcgcga caagaacccc
    25561 ctgttgcgta aactttatat cgaattgttc tcggaagcgc tggtcaacac ctccaacccg
    25621 gtgttcttct ccgacgagcg actggaacgc tatgccgagg tcgtgctgaa tgatcgcgac
    25681 ttcttcggcg cgcagggtct tgcctggtat cggcacgacc gccgccagtg gccggatgcg
    25741 gtgcgctggt tcaaatatgc ccgcgagtgg tcgaaggatg cggcgggcga tgccaagatg
    25801 gccgagggcc ttgccttctc gctgcgcaac ctcgatctct acgacgagag cgagatggtc
    25861 tccgtcgaat ggaaagacaa gcgcccggcg atgcgcaagc tctattccga gaccgtcggc
    25921 gaattcctcg tccggatgaa accggaattc tcctatccgg acgaccggat ggagcgcttc
    25981 ctcgacaccg tgacggcaga tgccaacaac atcggcgccc agtcggccgc gtggtattac
    26041 tacgctcgcg cgatgtatga tccggcggcg aactggttcc tccagagcct caaatggggc
    26101 ccggatttcg tccgcgatcc caagacggcc gaaggctaca tcacctcgct gcgcctcggc
    26161 ggccggcttg cggaagccga gctggccgcc ttcgactggt tcgacaagtc cccggcgctg
    26221 cgccagctct atttcgagat cgccggtcag ctgctggttg agatcaagcc tccgcagacc
    26281 ttcccggagg agcggctggc ccgctttgcc gaaatggcga cggaggcccg atcggtcacc
    26341 ggcgccattg cgctcgcctg gtaccaccag acccgctctg accacaaggc ttcggtcgag
    26401 tggttcgagc gggcgctgga atggggcggc gacacccccg atgtcgcggc ggtggagggc
    26461 tatgtcgttg ccctgatcgg cctcgaccgc ttcgaggatg ccgagaacct ggtgttcaag
    26521 tggcgcgatc gatccgacaa gatcgcccag ctctatatcg acgttttcgg cggcaagctg
    26581 ctcagctatc cgcagaacaa gcatatcgac ccggcccggc tcgaccgtta cgccatgctg
    26641 gccggcttcg agcgttccat ctacggcgcc caggcgctcg cctggtacta ctatgaccgc
    26701 cagacctggc ccgaagccgc ccgctggttc gaggccaacc tcacctggtc cggcaccggc
    26761 aaccacgacc agaaatcggt cgagggcttt gcccagagcc tgcgctatat cggccgccgc
    26821 gaggaagccg aagagattct ctacgcctgg cgcgatcgca acccgaccct gcgcaagctc
    26881 tatatcgaga tcatgtcgga ggggctggtc tcctcgtcca acccggtgtt cttctccgat
    26941 gagcggcttg aacgctacgc cgaggtcatt ctcaaggacc gcgatttctt cggcgcgcaa
    27001 ggcctggcct ggtatcgcca cgaccgccgg cagtggccgg atgcggtacg ctggttcaaa
    27061 tatgcccgcg agtggagcgc cgacggcaag ggcgacgcca agatggccga aggcctcgcc
    27121 ttctcgctgc gcaatctcga cctctacgac gagagcgaat tcgtctccgt cgagtggaag
    27181 gaccgctcgc cggcgatgcg caagctctat tccgagacgg tgggcgaata tctcgtccgc
    27241 atgaagccgg actataccta tcccgacgac cggatggcgc gcttcctcgc cgtcaccaac
    27301 gaggatgcca acaacatcgg cgcccagtcc gccggctggt actactacgc ccgcgagatg
    27361 tatgatccgg cggccaactg gttcctcgcc tcgctcaact gggggccgga tgtcgtccgt
    27421 gatcccaaga cggcggaagg ctatgtcacc tcgcttcggc tgggtggccg gctggtcgag
    27481 gccgaggctg cggccttcac tctgcgcgac cgctcgccgg cactcgcggc gatgtatttc
    27541 gacatcgccg gacaggttct gaccacgctg aagccgccgg agagtttccc ggtcgagcgc
    27601 ctcgcccgct ttgccgaact gtcctccgaa tcccgctcgg ttgccggtgc acaggcgctt
    27661 gcctggtatc atcaggcgcg cgaggaatgg agcgaggccg cctactggtt ccagaagtcg
    27721 atcgaatgga acgacaagac cgacgatccc aagtcgcatg aagggctgct ggtcgccctc
    27781 gtcggcctcg gccgctacga ggaagccgag aacctcatcc acacctggcg caccaccaat
    27841 cccgccatgg cggcgatgta tatggatgtc ttcggcggcg cgctgctgcg ctttcccagg
    27901 accgaacaaa tcgacattgc ccgtctcgac cgcttcggtg tgctggccgg cctcgagaaa
    27961 agcgtgttcg gcgcccaggc gctggcctgg tatcactacg agcgcgagaa ctgggccgac
    28021 gccgtgcgct ggttccagtc ctcgctcgac tggacgcagg ccggcaagca tgacgtcaag
    28081 agcatcgaag gcttcgtgca ggcgctgcgc catgtcggcc gccgcgatga ggctgaggaa
    28141 ctgctgttcc agtggcgcga tgccaattcc ggcatgcgca agctctatct ggagaccttc
    28201 ggcgaagcgc tgatcaacca gagcacgccg atcatcttcc ccgacgatcg catcgaccgc
    28261 tacgcgcata tcgcgctggc cgaccgttcg gctttcggcg cgcaggcact cgcctggtat
    28321 cgctacaacc gccgttcctg ggccgatgct gcccgctggt tcaagaatgc ccgcgcctgg
    28381 tctccggatg gcaagggcga tgccaagctg gccgaggggc tcgcttttgc cctgcgcaac
    28441 atggattcct acgatgaatc cgaagcggtc tcgtgggaat ggcgcgagcg ttcgccggcg
    28501 gtgcgcaagc tctataccga gacggtggcc gagttcatcg tccggctcaa gccgaatatc
    28561 ctctatccgg tcgatcgcct gtcgcgcttc cagaccatcg tcaccaacga caagtcgtcg
    28621 atcggcgcac aatcgattgc ctggtatttc cacgcccgcg agcgctgcgg cgaagccgtt
    28681 ccgtggttcc gcaacgcgct cgactggggc ccggatttcg tccgtgatcc caagaccgcc
    28741 gagggactgg caagctgcct gcggctgtcc ggcagcatcg acgaggccga gaccttcgcc
    28801 tattcttggc gcgaccgctc ggattcgctg cgcgagatgt atctcgaata cgccgccttc
    28861 gcgctcagcc gggtgcagcc accggcgacc tatccggtcg accgcatggc gcgctttgtt
    28921 gcgctcacca acaccttgaa gagccacgcc ggcgcacagg cgattgcctg gtatcgcttc
    28981 agccgcaagg aatgggacga ggccatcgtc tggttcaagc aggcgctgga ctggcagggc
    29041 gacaccaagg atgtgcgcac cgtcgagggc tacaccctcg ccctgcgcaa cgccggccgc
    29101 ttcgacgaag ccgagacgct ggcgctggcc tggcgtgacc gctcggactc gctcggccag
    29161 cagtatctcg aaatgctcgc cgagacgatg gccaggatgc cggcgaccga aaccctctcg
    29221 cccgatcgcc tgcagcgctt cagtcagctg gtcacggaac ggcaatggac ggccggcgcc
    29281 caggcgctcg gctggtatat ggtcaaccgc cagaagctcg gtgagggcga gacctggttc
    29341 aagaacgctc tcgcctggtc ggcggacggg cagaaggacc cgggcctgat cgagggctat
    29401 gcgctcgccc agaagctcca gaacaagtat ggcgaggccg acaccgccat tgtcgaactg
    29461 gtcgacaaat cggacaaggc gcgcgaactc tatcagctca actggatgga atggctggtc
    29521 aagaccaaac cgccggcgac cgttccgccg gagcggctga agaaattcgt cgatctggcc
    29581 actttcacca agcatgccgc aagcgcccgc tcgctcggct ggtatttcta tgaccgcaag
    29641 gactgggcac aggcccatgt ctggttcaag aacgccaccg actgggaagc cgacaagacc
    29701 aacgcgcagg gcgccgaagg ctacattctg accctgcgcc agatgggcaa ggacgaggaa
    29761 gccgaggccc tggcctatga ctggcgcgac cgctccgagg cgatccgtgc gctctacata
    29821 gactcgatcc ctgccatgct cgccaagctc ggtgagaccg gcaccttccc gaccgaacgg
    29881 ctcaaccgct tcgccgcgca ggtcacggcg gcaaaatccg cctatggcgc ccagtcgctc
    29941 ggctggtatc gcttccagcg caaggattat gcgcaggcgg tcggctggtt caaatccgcg
    30001 ctcgactggg ccggcgatgc caaggacccc aagctggccg aaggctatgc catggccctg
    30061 cgcgcctccg gccgtgtcga cgatgccgag acgatcgcct acgactggcg cgagcgggcc
    30121 gatggcctgc gcaatctcta tgtcgagatc ttcgccgaag ccctgaccaa gaacaacccg
    30181 ccgccggcct tcgcgccgga gcggctggcc cgttacgctg ccatcgtttc cgccgacaaa
    30241 agcgccaccg gcgcccaggc gctcggctgg tattcctaca atatcaagca gttgcgccct
    30301 gcccgggcct ggtttgaaaa agccatgcaa tggggcccga gcgaaggttc ggccctcggt
    30361 ctggcgctcg ctgcgcaggc gatgaacgac aaggccggct ttgccacaat catcaccaac
    30421 taccgcggca cctatccgaa gctgggcgat cttctcaatc cgcagaccgc caccgcgacc
    30481 cagaccctgg cggtgaccaa tgcgccgcgc atggtgctct ccaccgcccc gtcggtgcgc
    30541 tatgtcgagg aagtcgagac agtgaccgcg ccctcgtcgc gccgcgtggt cacgcgcgat
    30601 tcgcgcccgc caccgccgac agagaccacc cgcaagggcg ccaactgtca ttcgggtctt
    30661 gatccggtca atctcggctg gtgcctgctc aacaatgatc gcccgcagga agccgcacag
    30721 gccttcgagc gcggtcgcaa cggcccgaag gccgccgatg ccgcctatgg cgaaagcctt
    30781 gcccgcctgc gctccggcga aaccgacgaa gcgatccgct catcgatgtc ggcgccgatg
    30841 agccagaagc agcgcaccga cgtcacccag cagattctcg cccagcggat catcgcctcc
    30901 taccgggccg accgctacta tgacacgttg cgctaccttg atgagcgcgg cgcggtggcg
    30961 ccggaaacgc gtgatctggc gatgctgcgc ggctggtcgc tctacaagat cggccggacc
    31021 gatgcggccc ggcagatttt caccgagctt gatcgtcaat tgtcaaccaa agacacgcag
    31081 aatgcactct ccgtgctgat ggagattcgc ggcaaatact gaccgcgcga ggagttggat
    31141 catgctgagg ctggttactg tcggaatgct tgccgtcggc ctgtcgggct gcggcatggt
    31201 attcgactat tccgatccgg tgggcgatcc caagccgttc gataccacgc tgggcgtttc
    31261 gatcccaccc agtcaggcta tggcgatgct gccgcccggc gccgggcagg tcgtctcggt
    31321 gatcgagcgt cgtcgcggca acgccattca gcatgacatc acccttgccg gtgccccgcc
    31381 gaatttcggc gagaaccaga tcaatgtcac cgccttccgc tatgtcgagg aaagcgccga
    31441 gaaccccaag accgacatgc tcaagatcga gcgtcccgac gaagcgacga tctacgagga
    31501 aatgcaggag cgcatccccg gcggcgccct gccgacctcc aatgcggtgc cgcgcaatgc
    31561 catgggcaat ttcggctatg ccttcggccg ccgcaacggc gccaactgcc tctacgcttg
    31621 gcagtggctc gaatcgggcc aggcccgccc cttctcacct ttcgccggca agacacccgc
    31681 cccggtctcg gtccgcgtcc gcctgtgcag acccggagtg accgaggaag tcctggtcga
    31741 ttttgtccgc cagatgttcg tccagccccg ctcggatgat gcctatcggg tcagcgcatc
    31801 acgcggtccc gctccgatgg ccggctacgg cgccggcggc tatgggcggc cgggcatggg
    31861 cctcgccgcc acttcggggg cgtatggagc gggctatgga tatggcaatc cctacggcgg
    31921 cggctacggc gatggctacg gcccccgcta cgaggccccg cgctggcagg cgaccgggac
    31981 gacccgcgag gtggctgcgg ccccggtgcg ccgcaagaag attgtgcgcc gcattgtccg
    32041 tcgtgtcgtg ccgcaggaga ccatctccta ctcggccact ccggtgaccg tgccgtccag
    32101 ccggccggca acaatggtgg tctcgcccgg cggctattcc gccgtgccga tgccgcaata
    32161 ataccagcgg ccctgacaat tgaccgttag atcacgccgc attttgatgg aat
//
LOCUS       DAOMXG010000007        31352 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_962_length_31352_cov_11.198454, whole genome shotgun sequence.
ACCESSION   DAOMXG010000007 DAOMXG010000000
VERSION     DAOMXG010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 31352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31352
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_962_length_31352_cov_11.198454"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..836
                     /locus_tag="PLQ11_01010"
     CDS             54..836
                     /locus_tag="PLQ11_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008128196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRK23510.1"
                     /translation="MFSFRPLEPIGQGARIALGIAFFVLFFAAWGIATFGGFVPKTFL
                     ADPMTMVQDGWLLLTKFGFAADIGVTVWRVVGGFVLAALIAVPLGVLMGAYKPIEALL
                     EPMVSFARYLPASAFVPLLILWAGIGETQKLLVIFIGSVFQIILMVAVTVGNTRRDLV
                     EAAYTLGSTSGGVVKRVLIPSSAPDIAEILRLVLGWAWTYVIVAELIGSSSGIGHMII
                     ESQALMATGQIIFGIIIIGLIGLVSDFLFKMINRRFFAWRLA"
     gene            833..1627
                     /locus_tag="PLQ11_01015"
     CDS             833..1627
                     /locus_tag="PLQ11_01015"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRK23511.1"
                     /translation="MSEPVQLRVEGVGRIFPSVSGRPPVTALQPTDLAIAKNDFITLL
                     GPSGCGKSTLLRIVAGLDRPTSGRVLLDGKVVAGPGPDRGMVFQSYTLFPWLTVAENI
                     AFGLRERGVPEKERTPIITEYIAKVGLKGFENHLPKQLSGGMQQRTAIARALANDPEI
                     LLLDEPFGALDNQTRGLMQELLLGIWERERKTVIFVTHDIEEAVFMASRVLVMSARPG
                     RIKADVPVDLPHPRHYTIKTSPEFSRLKAMLTEEIRAEAVLAAAEH"
     gene            complement(1695..3071)
                     /locus_tag="PLQ11_01020"
     CDS             complement(1695..3071)
                     /locus_tag="PLQ11_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HRK23512.1"
                     /translation="MAHSVHPVDEVLPAPRLTALGLQHVLVMYAGAVAVPLIVGRALE
                     LTPQQIAFLINADLFACGLVTLVQSLGFPGVGIRLPVMMGVTFASVGPMLAMVSAGKA
                     AGLPKEQTLNLIYGSVIAAGIFGVLISPFISRMIRFFPPVVTGTIILVIGISLMRIGI
                     NWAAGAPVDTIPSYGDPVHLGVAAFVLLVILALTKFGSGFIANVAVLLGIIAGSVLAL
                     IIGKMGFDKVAAAPWFEFVMPFQFGMPIFDFWSIVTMCLVMIVVMIESLGMFLAIGTM
                     TGRTLSQDDLTRGLRADGVGTIIGGVFNTFPYTSFSQNVGLVGVTGVRSRWVCIAGGI
                     ILLLLGLTPKLAQLVESVPTFVLGGAGIVMFGMVAATGIRILSAVDYASNRNNLYIVA
                     ISVGFGMIPLVAPKFFQYMPKAVGPLLHSGILLAAIVAVILNLYFNGVKSQAEAEAEA
                     AASSHGSE"
     gene            complement(3154..3519)
                     /locus_tag="PLQ11_01025"
     CDS             complement(3154..3519)
                     /locus_tag="PLQ11_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23513.1"
                     /translation="MPAPPEIAAKLEHFPAGLNHCDDQFRVKDKKNATSVSFDPVGAF
                     FAGFGPKMIIPPGFRAAGARFGRWLGRIDGYTLRQPPPGSPRGPPNAMGSIWSESALA
                     QGCLAIQKIALKMILSLMT"
     gene            3518..3868
                     /gene="uraH"
                     /locus_tag="PLQ11_01030"
     CDS             3518..3868
                     /gene="uraH"
                     /locus_tag="PLQ11_01030"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010686664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HRK23514.1"
                     /translation="MARLSTHVLDTSIGRPAQGVKLRLARVTERGETEVATATTNSDG
                     RTDQPLLSGQAYQPGTYVLYFDVREYFSARTPPAASPFLDVVPIRFSMVEPDGHYHVP
                     LVVTPWSYSTYRGS"
     gene            3872..5311
                     /gene="puuE"
                     /locus_tag="PLQ11_01035"
     CDS             3872..5311
                     /gene="puuE"
                     /locus_tag="PLQ11_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase PuuE"
                     /protein_id="HRK23515.1"
                     /translation="MAYPRDLIGYGAHPPHPRWPGEARLAVQFVINFEEGGENNILHG
                     DEASEAFLSEIVGAAPWPGQRHMNMESIYEYGSRAGFWRLHRMFTDRALPVTVFGVAS
                     AMARHPDAVRAMVAAGWDIATHGLKWIDYRSLPAATEAAHIAEAIRLHQELTGRRPNG
                     MYQGRTSENTLRLTQEEGGFLWSADSYADELPYWVEGPNGRGGTGPFLMVPYTLDAND
                     MRFATPQGFNAGDQFFSYLKDSFDTLYAEGEAAPKMLSIGLHCRLVGRPGRAAALARF
                     LDYVKGHDRVLVATRQAIAEHWVMNHPPAGGWSPSRLSRPLFVARFGGIFEHSPWVAE
                     KAFDAGLGPETDTVGGLHAAMVKAMRAGTQSQQRDLILKHPDLAGKLHAAKQLTADST
                     AEQASAGLDHLTDADRKRFTELNDGYKARFGFPYILAVKGKTKAEILANFEERLTNSA
                     QQEFATALAQIERIALLRLTDMPGLGVRA"
     gene            5308..5796
                     /locus_tag="PLQ11_01040"
     CDS             5308..5796
                     /locus_tag="PLQ11_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HRK23516.1"
                     /translation="MIRLPIRPLTREAFAPFGTVIETTGEGLTINAGFARRFNDRCKV
                     DVGCEGGETNLSIFIARARALPLTIPMMEMHPLGSQAFLPLQDAPWLVVVCTDPDDPA
                     SYHAFACQGTQGVNYARGTWHFPLITFGEGDRFIVVDRKGPGNNLLEHDLAAHRQPIV
                     TD"
     gene            5806..6642
                     /locus_tag="PLQ11_01045"
     CDS             5806..6642
                     /locus_tag="PLQ11_01045"
                     /EC_number="3.5.3.26"
                     /EC_number="3.5.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004037 - allantoicase activity [Evidence
                     IEA];
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional allantoicase/(S)-ureidoglycine
                     aminohydrolase"
                     /protein_id="HRK23517.1"
                     /translation="MSRRPYFAPHGGLPPQTQLLTDRAMFTPWYGVIPKGTFSDIVTS
                     YLPFWDKTRLWVIARPMSGFAETFSHYIMDVAPGGGSTRPEADPEAQGVLFVVEGGLC
                     LTIDGKAHDLGAGGYAFLPAGCRWTLANPTSQPVRFHWIRKRYQAVEGLGHPEAFVTS
                     DQAVKPIPMPDTNGAWATTRFVDPSDLRHDFHVNIVNLEPGAVIPFAETHVMEHGLYV
                     LEGKAVYRLNQDWVEVEAGDYMWLRAFCPQACYAGGPGRFRYLLYKDVNRHMPLGPFQ
                     QR"
     gene            complement(6646..7866)
                     /locus_tag="PLQ11_01050"
     CDS             complement(6646..7866)
                     /locus_tag="PLQ11_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020094529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HRK23518.1"
                     /translation="MEAYLMDWGSMLLRWLHVVTAIAWIGASFFFMHLDASLKPDPSI
                     KAGGATWQVHGGGFYEMRKYFVAPDTLPAEITWHKWQSYWTWISGFILLVWVYYAQAT
                     LYLIDPSVMALKPWQASAIGLVSLALGWVVYDQLCKSPLGKNDNLLALAGFGFVVAMS
                     WFYTQVFSGRGALIHTGALMATMMTANVAMIIMPNQRKVIAMLEKGETPDPKYGKQAK
                     QRSAHNNYITLPVLFMMLSNHYPMVWANAAVIPAIVVLIIVAGALIRHFYNIRHTDHA
                     KSPWWTWAAAAIALGIAFYVAMAASPGGRQRLGLAPRETPNVMLAGLRPAPAHVSDII
                     VSRCSMCHAPEPVWDGIQIAPKGVMLDTPERIALQADAIRMWSVLTHAMPPNNLTEMT
                     GEERRTLADWLKSL"
     gene            8028..8810
                     /gene="hyi"
                     /locus_tag="PLQ11_01055"
     CDS             8028..8810
                     /gene="hyi"
                     /locus_tag="PLQ11_01055"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HRK23519.1"
                     /translation="MPRFAANLSMLFNEHAFLDRFEAAAKAGFLGVEFLFPYAEDKAV
                     LAEKLAANGLTQVLFNLPPGNWDAGERGIACLPDRVGEFQDGVGKAIDYARALGNRQV
                     NCLSGIAPAGVPREQMLRTLVENLRFAAEKLKEVGILLIVEPINFYDMPGFFLNRSSE
                     AVAIFDEVGSDNIKLQYDIYHMQRMEGELAATIERLLPRIAHIQIADNPGRHEPGTGE
                     INYPFLFALLDRLGYPGWVSCEYRPKAGTLAGLGWMAGTSRV"
     gene            complement(9122..10129)
                     /locus_tag="PLQ11_01060"
     CDS             complement(9122..10129)
                     /locus_tag="PLQ11_01060"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRK23520.1"
                     /translation="MKRSKIRLSDVARDAGVSPATVSRAISQPELLSQATLARVRRSA
                     ERLGYRPDAAARALASGRSLTIGAVMPTLDNAIFSRALQAMQARLATEGYQLLVASHD
                     YNLAAETEAIRTLLSRGVDGLILVGAERASEASALLAAADVPVVLTWRAPPGAPAVVV
                     DNELAGDMAARHLIALGHRRIAVFTGAIRFNDRQRARQLGVLRALEAAGLTLPPSMIC
                     EAPTTLAGGRMGCTKLLELAEPPTAIICGLDLLAIGCMVEAQARGLAVPNDVSVVGID
                     DLDMSAHVSPSLTTVHVPTARIGAEAAETLIAMLRHQDANTSICLPVELIVRRSTGRC
                     P"
     gene            10303..11088
                     /locus_tag="PLQ11_01065"
     CDS             10303..11088
                     /locus_tag="PLQ11_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRK23521.1"
                     /translation="MASVGSRRTMIGLATLAGLIVLWFLLTTVTGLVTGARFPSPSEF
                     WTSLTQISGRGYAGASLATHALHSLKLVAMGFSFAVLTGIPLGLWMGWSRRVEAFVNP
                     IFLIIRPIPPLAWIPLAILWLGLGDAAKVMVIWFSAFVPSVINAFTGVRNIERPVIEA
                     MQMLGTPRWRMIREVIVPAASPMIFTGLRLSLQASWTTLVAAELVGALVGVGFVLNMA
                     QQDIYPGMILVGMITVGILGWATTMLLGWAERRALAWNVAARD"
     gene            11092..11880
                     /locus_tag="PLQ11_01070"
     CDS             11092..11880
                     /locus_tag="PLQ11_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRK23522.1"
                     /translation="MTGRPLSSLETVLLGLAGLCAFVGLWTLLSVTGIVPRQFLPTPL
                     DVATRMIQLLSSPFAGATLPMHLASSFQRYAYGVVLAALIGVPLGLLMGWFRLLDDIV
                     TPIFDGLRFIAPIAWVPFAALWFGTGIGGPVMIIFAGAFPPCLINAYRGARFVEPRLI
                     EAGRMLGTPNARMILEVLLPAAVPSIIAGLRVSAGLGWQSLVGAELIVAAAGVGFMMV
                     QAQGSVQTATVMVGMVAIGLVGMLIDVLLRQGEGWLRRRRGLEA"
     gene            11890..12687
                     /locus_tag="PLQ11_01075"
     CDS             11890..12687
                     /locus_tag="PLQ11_01075"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRK23523.1"
                     /translation="MGSISFRKVDKVFGSSSGGVKALDDINLEIADKEFVAIVGPSGC
                     GKTTCLRMVAGFEAASSGEVLVNGKAVTEPGPDRAVVFQQFALFPWKTVRENIELGLR
                     NKNLPAPKRDELIAGALKLMSLESHANAFPHQLSGGMQQRVAIARAYVLDPVVLLMDE
                     PFGALDAQTRVVMQEELVRLARRNPRTVLFITHAVEEAVYLADRVAIMTRRPGRIKEV
                     LDIKTIRETENWDSHSKIEDVMDLESFVHLRTHIWKSLREEKGFKDV"
     gene            12680..13927
                     /locus_tag="PLQ11_01080"
     CDS             12680..13927
                     /locus_tag="PLQ11_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRK23524.1"
                     /translation="MSEPRRLVRDLTEPEPIPEAGIARAVELMRSGRLFRYGEMGADQ
                     LDVSLLETEFAAFAGRRYCVAVNSGGCALFLALKVAGVAPGDPVLVNGFTLAPVPGAI
                     LHAAARPVIVEIGTDYVIDPDDLRRKAQASGARVLMLSHMRGHIADMGAVMAVAEECG
                     LAVIEDCAHAMCASWQGRAIGTFGVAAAFSAQTYKHLNAGEGGFLVLDDPAMAARAIL
                     HSGSYMLHGQHLSSPGEAALAAAGDHVPNFSMRMTALAAAMLRPQLKELPRRVARWNA
                     IHDRIAAGLQRSQHLHVPERGGGQAYSATSVQFSVSGLEAAEIERFLGLARTRGVPIK
                     WFGAERQTGFTSAPRHWRYAGDQGALAPTHRVLASLCDIRTPVTLSDEDCDLIAEIVR
                     ESIETVAADRVASNGKQTTLLPA"
     gene            13955..15004
                     /locus_tag="PLQ11_01085"
     CDS             13955..15004
                     /locus_tag="PLQ11_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010686705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRK23525.1"
                     /translation="MKRILTGLALATALATTMPLTASAQTAIGVSYQPSLYWALPFHY
                     ANVKGWWKDVGLAPNFTTFPAGAPQIAAAPSKSWDVGGTGSVPAVLGAVRFNILTIGI
                     TNDESKTNAMMVVGSKFDAIKANPASLKGQRLLLTTNSTVDYASRKCLVKMGLAANDM
                     QFVNLGQAQIITALTSNNGDIAGVWAPNTYTLEDNAGAKYLCSGADANAIVPGALIVR
                     GDFAKERPDDVAKFLAVYLRGWSWAKANPAEARTLALDFYKQGGLEVSARAMDQEFAL
                     RPTFGLDEQLKILDRSASGSTVDGWFSEIGKFITEVGTIPANPDAKSYITNEFMKKVA
                     ADPKLKAFATEFDKK"
     gene            15088..16401
                     /gene="hisD"
                     /locus_tag="PLQ11_01090"
     CDS             15088..16401
                     /gene="hisD"
                     /locus_tag="PLQ11_01090"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010686704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HRK23526.1"
                     /translation="MAVTYLKKAAKTPETETDAARKVVTEMLAAIEAGGEAAVKDYAF
                     TLDRWSGPIVMDEAMIAERTRDVPQSVKDDIRLAAGQVRRFAEAQKASCKDFSIELSP
                     GLQLGQKMVPVNTAGCYVPTGRYAHIASAYMSIATAKAAGVKTVVACSAPFKGTGIHP
                     HVLYAMTVAGADVIMCLGGVQAIAAMTYGLFTGKPADIIVGPGNKFVAEAKRMLFGKV
                     GIDVFAGPSEVAVLADDSADPLIVATDLVGQAEHGHESPAWLITDSRRIADEVSALVP
                     KLIATLPPTARDAAGAAWRDYGEVVLCGSREEMVRVSDAYACEHLEVHCTDLDWWHAN
                     LTNYGSLFLGEETNVAFGDKVSGPNHILPTKFAARYSAGLSVHKFLKPLTWQKMSRDA
                     CATLAPVSARISRLEGMEAHARTSDVRMAKYAPGMQIELGRPVES"
     gene            16402..17166
                     /locus_tag="PLQ11_01095"
     CDS             16402..17166
                     /locus_tag="PLQ11_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010686703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRK23527.1"
                     /translation="MSFRLTHLFDLSGRKALVTGGNSGIGLAMARALGLAGAKVILVA
                     RREAELAQAATGLRQDGIDAVAVAADLADAAVGPSLASCCGTDIDILVNAAGVNLRQP
                     FAEVTAAAFDLHMALHLRAPFLLTQAFAPAMAARRWGRIINLASLQSSRAFPNSAPYG
                     AGKGGIVQLTRAIAEEWSRHGVTCNAIAPGFFPTPLTAPVFGDPERAARNAAQTAIGR
                     NGDLDDLTGATVFLASDAAAYVTGQTLAVDGGFTAK"
     gene            17176..18171
                     /locus_tag="PLQ11_01100"
     CDS             17176..18171
                     /locus_tag="PLQ11_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HRK23528.1"
                     /translation="MKALVYTGPNSLAYRDEPDPVPQAGEVLVRIEAVGICGSDMHAY
                     HGHDSRRPAPLILGHEAAGRIVGGARDGERVTINPLVVDPACPYAIEGRWHLSPTRQI
                     ISMPPRPGAFAEYVRVPAPNAVAVPATMPITHAALAEPVAVSWHAVRIGMQQLHQPLA
                     CCRVVVLGGGAIGLAAAIVARHFGAKDIRIAETNPLRTRLIETEEGFTVYAPGSAGAP
                     ADNTIDLVIDAVGANATRAAASAMVRPGGVIVHAGLLPGAEGLDIRKITLQEIIVTGT
                     YCYTPVDFHQTIAALASGALGSLTWFAERPLADGAQAFRDIDAGVASAPKIVLRC"
     gene            complement(18294..19280)
                     /locus_tag="PLQ11_01105"
     CDS             complement(18294..19280)
                     /locus_tag="PLQ11_01105"
                     /inference="COORDINATES: protein motif:HMM:NF014715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HRK23529.1"
                     /translation="MRVAFPALLADIGGTNVRFAMLARANAPLSDCLRFETGGVPDCA
                     AAVQMACRKAGWPMPASLLLGAAGAISGRSVRLTNAAWVIDGPALAQRLGLRQGLLLN
                     DFETLALALPALASDDLMPIGAGLSGHGTRLIVGPGTGLGVGALIRAGQTLMPVSSEG
                     GHAALAAGSPAEHRLFARLAADGRSLSAESLLAGPALTRLRLAFDPACPPDAARPPAI
                     VEAALEGDPACRQVIDLWLTLLARFCAGMALTFLATGGVFLAGGILPRLRSLIDAAAF
                     RAAFEDNSAHAELLGRIPVQLVTAQEPGLIGLEALARAPERYLLDYAGRAWC"
     gene            complement(19285..20367)
                     /locus_tag="PLQ11_01110"
     CDS             complement(19285..20367)
                     /locus_tag="PLQ11_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003597542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HRK23530.1"
                     /translation="MSVRLIGLAAVLASSVAWAQDRAVNVYNWNDYIDPKVLEDFTRE
                     TGIKVVYDTYDNNEIVETKMLTGKSGYDIVVPSGPFLQRLIQAKVFEKLDQARLPNLK
                     HMWAEVRQKLAVYDPGNVYAVNYLWGTTGIGINVAKVKERLGDMPLNSWDLVLKPQVV
                     EKLKDCGVYMLDAPEDVFPGVLRYLGLEPASKKPEDITKAADHLMKVRGAVRKFHSSE
                     YINALAAGDICVALGYSGDVLQARRRAEEANNGVKIDYLIPKEGALMWFDSFVIPADA
                     PNKGNAYAFLDFMMRPDVAARNSNMVSYASGNLAAKAEIKKEILDNPNIYPDAATFAR
                     LFTNTAYDERVQKVVTRQWTRVKTGK"
     gene            complement(20452..21597)
                     /locus_tag="PLQ11_01115"
     CDS             complement(20452..21597)
                     /locus_tag="PLQ11_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HRK23531.1"
                     /translation="MNSVFSRLPTTIFDTMSRLAREHQAVNLGQGFPDDPGPLDVRQK
                     AAEAVVHGWNQYPPMFGLPELRKAVARHYQRFQGITVDPEREVMITSGATEAIAGALL
                     ALIEPGDEVVLFQPMYDAYLPLVRRAGGVPRFVKLRPPHWRFTESDLAAAFTDRTRVV
                     LFNNPLNPAGVVYDREDLELLARFCVRHGAIAVCDEVWEHVIFDGKHHVPLIGLPGMA
                     ERTVKIGSAGKIFSLTGWKVGFVLAAPPLMQVLSKAHQFLTFTTPPNLQAAVAYGLDK
                     EDSYFAGMRQGFQRSRDRFVAGLTALGFDVLTSTGTYFVNVDISSLGETDDAAFCQRL
                     VVQEGVAAIPVSAFYAEDAVRSVVRFCFAKADTTLDAALDRLARLKR"
     gene            21897..23159
                     /locus_tag="PLQ11_01120"
     CDS             21897..23159
                     /locus_tag="PLQ11_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008598226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HRK23532.1"
                     /translation="MRVDGQRLWRRLEAMAAITEPDRPYTRRSFTPRFIEGRAWIADA
                     FRGAGLVTQIDAGGNLIGRLGGSDNNLKPIVIGSHSDTVPSGGRYDGVLGVLAALEVA
                     EALQAAGKRLRHPLEVVDFLAEEPSEFGLSCIGSRAWAGALSPADLDRRRPDGMTLAE
                     AIAYVGGDPSRLAGVRRAKGGTAAYLEVHIEQGIVLERSGTPIGIVTDIVGIRRERWM
                     ISGRADHAGATPMNLRKDALVGAAHVIGEASAAATATLGTAKPLVATIGHIEVEPNAA
                     NAVPGRVVMTLEVRSGDDAAVRRFGDTLVERTKDRLAALGLELARSEISHALPTPCAD
                     VIRSAIRHAAHDLGLATVDLPSGAGHDGVFVAATGPVGMIFTPCEGGRSHAPEEAMTP
                     EQAESGAAALLAAVLRLDETLPTDSLPT"
     gene            complement(23248..24381)
                     /gene="dnaJ"
                     /locus_tag="PLQ11_01125"
     CDS             complement(23248..24381)
                     /gene="dnaJ"
                     /locus_tag="PLQ11_01125"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HRK23533.1"
                     /translation="MPKRDYYEVLGVQKNADEAALKSAFRKLAMQYHPDKNPGDKAAE
                     AKFKELNEAYQCLSDSQKRAAYDRFGHAAFEQGGGGQGPFGGGGGGFSGMGDIFEDLF
                     GEVFGGGRRSSGGRERGADLRYNLEITLDDAFRGKAATIKVPTSITCEACSGSGAKAG
                     SKPKRCATCGGHGRVRATQGFFSIERTCHACHGRGETIDDPCRACNGGGRVTRERTLS
                     INVPAGVEDGTRIRLSGEGEAGLRGGPSGDLYVFLSVKPHGLFQRDGADLFARVPISM
                     VAAALGTEIAVPTLDGTDAKVRIPEGTQSGKQFRVKGKGMPVLRSKDRGDLFIQTVVE
                     TPQNLTRRQKELLREFEQGSADGTHPESEGFFARMKKDFFGGQ"
     gene            complement(24474..25394)
                     /locus_tag="PLQ11_01130"
     CDS             complement(24474..25394)
                     /locus_tag="PLQ11_01130"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020092420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HRK23534.1"
                     /translation="MAFAGGGALASAVTGAGGLGLIGGGYGDDTWLDEQWRLAGPSAI
                     GVGFITWSLRKSPALLTAVLRHRPKAVMLSFGDPRPFVDEIRAAGAAIICQCQSLDHV
                     WDAIDIKADVIVAQGTEAGGHGALRGLVSFVPEAADLIRAEAPEAILVAAGGIADGRG
                     LAAALALGAEGVLVGTRFWASREALVHPRHHHAIVEATGDETIRTSVVDIARQIAWPP
                     GFTARVRRNAFTSRWHGHEAALADAIAAEAPRYRQAFVEGDPDNTGVWFGEAAGLIAD
                     IQPAAAIVQSMTSDALAILRRLGGQDAPTG"
     gene            complement(25563..27470)
                     /gene="dnaK"
                     /locus_tag="PLQ11_01135"
     CDS             complement(25563..27470)
                     /gene="dnaK"
                     /locus_tag="PLQ11_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015823233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HRK23535.1"
                     /translation="MGKVIGIDLGTTNSCVAVMDGSTPKVIENSEGARTTPSIVAFTD
                     DGERLVGQPAKRQAVTNPERTFFAIKRLIGRTFNDPMTQKDMGLVPYKIIKAGNGDAW
                     VTADGKQYSPSQISAFTLMKMKETAEAHLGQPVTQAVITVPAYFNDAQRQATKDAGKI
                     AGLEVLRIINEPTAAALAYGLDKKKAGTIAVYDLGGGTFDVSILEIGDGVFEVKSTNG
                     DTFLGGEDFDMRLVEYLVDEFRKEQGIDLKKDKLALQRLKEAAEKAKIELSSSAQTEI
                     NLPYITADASGPKHLTLKLTRAKFESLVDDLIQRTVEPCRKALKDAGLSAGQIDEVVL
                     VGGMTRMPKVQDVVKQFFGKEPHKGVNPDEVVAIGAAVQAGVLQGDVKDVLLLDVTPL
                     SLGIETLGGVFTRLIDRNTTIPTKKSQVFSTAEDNQQAVTINVFQGEREMARDNKSLG
                     RFDLDGIPPAPRGMPQIEVTFDIDANGIVSVTAKDKATGKEQQIRIQASGGLSDADIE
                     KMVKDAEANAAEDKKRRELVEARNQGEAMIHSAEKALKEHGEKVGAAEKEAIEKAVSE
                     LREVVSGDDAEVIAARTEGLMQASMKLGEAMYKASQEEAAHADAAKHAAKDDVIDADF
                     KEVKKDDKKGA"
     gene            27662..27913
                     /locus_tag="PLQ11_01140"
     CDS             27662..27913
                     /locus_tag="PLQ11_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23536.1"
                     /translation="MIPPSVIVTSVVAIAGTLWLGSLVLKSMTAKRKIADTRYGRRSD
                     GTVTSMSDGSSGEGGRSASSSDSDSCSGDSGGDGGGCGD"
     gene            28029..28178
                     /locus_tag="PLQ11_01145"
     CDS             28029..28178
                     /locus_tag="PLQ11_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23537.1"
                     /translation="MSGFVVLGLVLFLGGTAMALVRMNRLSCMPCNSADDSAYCNPPR
                     PHRPA"
     gene            complement(28183..29124)
                     /locus_tag="PLQ11_01150"
     CDS             complement(28183..29124)
                     /locus_tag="PLQ11_01150"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006936919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HRK23538.1"
                     /translation="MLPNRSPAFPSPPALRLLKRWTPRLAGPSTPHRFGHSAPLPAGS
                     LCRLGSLEVRLAQDAGDLRAVQRLRYEVFFNECQAVPTARMLLARRDADRFDRICDHL
                     MVIDHDGQGLSGRSARPKVVGTYRLLRGDVADAHGGFYSAQEYDLRGLMRAHPGARVL
                     ELGRSCVLKPYRTKRIVELLWAGIWAYVRHHRMDMMIGCASLPGTDPAALALPLSFLH
                     HHARAPDAWQVAALPDRYVPMNRLPAEAVESRAALGVLPPLVKAYLRLGGRFGDGAVV
                     DRAFGTTDVLVIVRVADIAPRYIDFYGARREGRGEED"
     gene            complement(29213..>31352)
                     /locus_tag="PLQ11_01155"
     CDS             complement(29213..>31352)
                     /locus_tag="PLQ11_01155"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRK23539.1"
                     /translation="ARLTTLDTGLPVPDVNRSSVHQSATLGAASSFDEGQAMNADGLF
                     LSTATWMAVAIVALAVAGLLTGTAIFLIRDMARLRKRARSIMRHVSELDCARAEAEAR
                     LKAASSAVESAGAFLIICDTDGTIRDSNQAYHDFCIRHGLDPLRFDLVGEAGRSRDRN
                     GPIDSVLAGVSVRWTIGMARAGQGAQRCVAIGQPVAAKPQMEDQNGAKSRFLATISHE
                     MRTPLNGVIGMAGLLDDTALTLEQRNYVDAIRSSGEALLSLINEILDYARLGAGRLER
                     VQERVAIAPLVESVVELLAPRAQDRGIEIATLVSPDVPPVVLGDAARLRQVLTNLAGN
                     AVKFTERGGVGLRVESQQGGMIAFTVADTGPGIAIERQQAVFEDFEQADSDTGRRHGG
                     SGLGLAISRRIVTHLGGTIALISRPGAGSIFRVALPLPAASEAAAEVAVPNAVLAGQR
                     VVIVSASPFEAPFLAERLSRLGARPRLATTTGQGLSCLADADVLVVDAALGSDACRRL
                     TDAAAAAGVSRRIVLLSPFERRSFGAPADAGFDGYLVKPVRARSLIARFASDNGPEPA
                     AGDELQATPAKPLAGRRLLLAEDNPVNALLARTLIERLGGDVVWVKDGLEALARLEAP
                     DEGMFDLALFDVRMPGLDGIDLTRKLRQRESMGGAPHLPVIALTANAFAEDRAACLAS
                     GFDAFLSKPLDRAVFEQTLADVLKNPHKAA"
BASE COUNT         5597 a        10049 c         9963 g         5743 t
ORIGIN      
        1 cccgatgggg gccggcggca gcgccggccc ctcccccgct tcagggaccg gcgatgttct
       61 ccttccgccc gcttgaaccc atcggccagg gtgcccgcat cgcgctcggc atcgccttct
      121 tcgtgctgtt ctttgcggcc tggggcatcg ccaccttcgg cggcttcgtg cccaagacct
      181 ttctggccga ccccatgacc atggttcagg acggctggct gctgctgacg aaattcggtt
      241 ttgccgccga catcggcgtc acggtgtggc gtgtggtggg cggcttcgtg ctggccgcac
      301 tcatcgccgt gccgctcggc gtgctgatgg gcgcctacaa gccgatcgag gcactgctcg
      361 aaccgatggt gagttttgcg cgctatctgc cggcctcggc gttcgtgccg ctgctgatcc
      421 tgtgggccgg catcggcgag acgcagaagc tgctggtcat cttcatcggc tcggtattcc
      481 agatcatcct gatggtggcg gtaaccgtcg gcaacacccg gcgcgatctc gtcgaggcgg
      541 cctatacgct gggctccacc tcgggtggcg tcgtcaagcg tgtgctgatc ccctcgagcg
      601 cgccggacat cgcggaaatc ctccggctcg tgctcggttg ggcctggacc tacgtcatcg
      661 tcgccgaact gatcggctcg tcctcgggca tcggccacat gatcatcgag agtcaggcgc
      721 tgatggccac cgggcagatc atcttcggca tcatcatcat cggcctgatc ggtcttgtct
      781 cggatttcct gttcaagatg atcaaccgcc gcttcttcgc ctggaggctg gcatgagcga
      841 gcctgtgcaa ttgcgtgtgg agggcgtcgg ccgcatcttt ccatcggttt ccggtcgccc
      901 gccggtcacg gcgctgcaac ccaccgacct ggctattgcc aagaatgatt tcatcacatt
      961 gctcggcccg tccggctgcg gcaagtcgac cctgctgcgc attgttgccg gcctcgaccg
     1021 gccgacgtcg ggtcgcgtgc tgcttgacgg caaggtcgtg gccgggccgg ggccggatcg
     1081 cggcatggtg ttccagagct acacgctgtt cccctggctg acggtggccg agaacatcgc
     1141 cttcgggctg agagaacgcg gtgtgccgga gaaggagcgc acgccgatca tcaccgaata
     1201 catcgccaag gtggggctga aaggcttcga aaaccacctg cccaagcagc tttccggcgg
     1261 catgcagcag cgcaccgcca ttgcgcgtgc gctcgccaac gatcccgaaa tcctgctgct
     1321 ggacgagcct ttcggcgcgc tcgacaacca gacccgcggg ctgatgcagg aattgctgct
     1381 cggcatctgg gagcgcgagc gcaagacggt gatcttcgtc acgcacgata tcgaggaggc
     1441 ggtgttcatg gcctcgcgcg tcctcgtcat gtcagccagg ccgggccgca tcaaggccga
     1501 tgtgccggtc gatctgccgc atccgcgtca ctacaccata aagaccagcc cggaattctc
     1561 gcgcctgaag gcgatgctga ccgaggaaat ccgcgccgag gccgtgctgg cggccgccga
     1621 gcattgacgg cgacaacgaa ccgccggagg ccaagcctcc ggcgctccgt ttgccttgcc
     1681 cgacaggccg tgccctactc gctgccgtgg cttgaggcgg ccgcctccgc ctcggcttcg
     1741 gcctggcttt tcacgccgtt gaaatagaga ttgaggatca cggcgacgat tgccgccagc
     1801 aggatgccag agtgcagcag tggcccgacc gccttgggca tgtactggaa gaacttcggc
     1861 gcaaccagcg ggatcatccc gaagccgacc gaaatggcga cgatgtagag gttattgcgg
     1921 ttgctggcat aatccaccgc ggagaggatg cggatgccgg tggccgccac catgccgaac
     1981 atcacgatac cggctccgcc cagcacgaag gtcggcacgc tttccaccag ttgggccagt
     2041 ttgggcgtca ggcccagcag cagcaggatg ataccgccgg ctatacagac ccagcgcgaa
     2101 cgcacgccgg tcactccgac cagcccgaca ttctgcgaga aggacgtgta ggggaaggtg
     2161 ttgaacacgc cgccgatgat cgtgccgacg ccatcggcgc gcaggccgcg ggtcagatcg
     2221 tcctgactga gagtcctgcc ggtcatggtg ccgatcgcga ggaacatgcc gagcgactcg
     2281 atcatgacga caatcatcac caggcacatg gtcacgatcg accagaaatc gaatatcggc
     2341 atgccgaact ggaacggcat tacgaattcg aaccacggcg ccgcagcaac cttgtcgaac
     2401 cccatcttgc cgatgatgag cgccagcacc gacccggcaa tgattccgag cagtaccgcc
     2461 acgttcgcga tgaagccgga cccgaatttg gtcagcgcca ggatcaccag caggacgaag
     2521 gctgccaccc cgagatgcac cgggtcgcca taggacggga tcgtgtccac cggggcaccg
     2581 gcggcccagt tgataccgat gcgcatcagc gagatgccga tcaccaggat gatcgtgccg
     2641 gtcaccaccg gcgggaagaa gcggatcatc cgggagatga acggcgatat cagcacgccg
     2701 aaaatgccgg cggcaatcac cgatccatag atcaggttca gcgtctgctc cttgggcagc
     2761 cccgcagcct ttccggccga aaccatggcc agcatcggtc cgactgacgc gaaggtcaca
     2821 cccatcataa ccggaagacg gataccgaca ccgggaaagc cgagcgactg gaccagcgtg
     2881 acgaggccgc aggcgaaaag atctgcattg atcagaaacg cgatctgttg cggcgtcagc
     2941 tccagcgccc ggccgacgat cagcggaacc gcaacagccc cggcgtacat gaccagaacg
     3001 tgctggagcc ccagcgccgt caatctgggt gccggcaaaa cctcgtctac aggatgcacc
     3061 gaatgggcca tcaaagcctc ccccttgttg cttccggcgg tcaagcgccg cccggtatcg
     3121 ttcgccccac tgtcgccaga gcgcgagggt ggattaagtc atcaaactca ggatcatttt
     3181 caaagcaatt ttttgaattg ccaggcaacc ctgtgctaga gcactttccg accagattga
     3241 acccattgcg tttggcggac cgcgtggcga tccgggcgga ggttggcgaa gagtgtatcc
     3301 gtcgatacgg ccgagccagc gtccaaaccg ggcgcccgcg gcccggaaac ccggagggat
     3361 gatcattttc gggccaaatc cggcgaaaaa tgctccaacc ggatcgaagg acacgcttgt
     3421 cgcatttttc ttgtccttga cccgaaactg gtcatcgcaa tgattcaatc cggccggaaa
     3481 gtgctctagt ttcgccgcaa tctctggagg agccggcatg gcgcgattgt ccacacatgt
     3541 tctggatacc agcatcgggc gcccggcaca gggcgtgaag ctgcggctcg cccgtgtcac
     3601 ggagcgtggc gagaccgagg ttgcaaccgc aaccaccaac agcgacgggc ggacggatca
     3661 gccgctgctg tccggtcaag cctaccagcc gggaacctat gtgctgtatt ttgatgtgcg
     3721 ggagtatttt tccgcgcgga caccgccggc ggcctcgcct tttctggatg tcgttcccat
     3781 tcgcttttcc atggttgagc ccgacggaca ttatcatgtg ccgctcgtcg tcaccccgtg
     3841 gagctattcg acctatcggg gatcctgacg gatggcttat ccgcgcgatt tgatcggcta
     3901 tggcgcccat ccgccgcacc cgcgctggcc gggcgaggcc cgcctcgccg tgcagttcgt
     3961 catcaatttc gaagagggcg gcgagaacaa tatcctgcat ggagatgagg cctccgaagc
     4021 cttcctctcc gaaatcgtcg gtgcggcgcc gtggccgggc cagcgccaca tgaacatgga
     4081 atcgatatac gaatacgggt cgcgcgccgg tttctggcgg cttcaccgga tgttcaccga
     4141 ccgcgcgctt ccggtgacgg tgttcggtgt tgccagtgcc atggcacgcc atcccgatgc
     4201 agtacgggcg atggtcgcgg ccggctggga tatcgccacc catggcctca agtggatcga
     4261 ttatcgctcc ctgcctgcgg ctaccgaggc cgcccatatt gcagaggcga tccgtctgca
     4321 tcaggagctg accggccgac ggcccaacgg aatgtatcag ggccgcacct cggagaacac
     4381 tttgcgtctg acccaggaag aaggtggctt tctatggtcg gccgacagct acgcggatga
     4441 actgccctat tgggtcgagg ggccgaacgg gcgcggcggc accgggccgt ttctgatggt
     4501 gccctatacg cttgatgcca atgacatgcg ctttgccacg ccgcagggct tcaacgcggg
     4561 cgatcagttc ttttcctatc tcaaggacag tttcgacacc ctctatgccg aaggcgaggc
     4621 cgcgccgaag atgctgtcga tcggcctgca ctgccgcctc gtcggccgac cggggcgcgc
     4681 ggcagcgctg gcgcgttttc tcgattatgt aaaaggccat gatcgcgtct tggtggcgac
     4741 ccgccaggcg atcgccgagc actgggtgat gaaccatccg ccggccggag ggtggtctcc
     4801 gtcgcggcta tcgcgcccgc tgttcgttgc gcgcttcggc ggcatattcg aacattcgcc
     4861 ctgggtggcg gaaaaagcct tcgatgcggg gctgggaccg gagacggata cagtcggcgg
     4921 attgcatgcc gccatggtca aagccatgcg cgccggcact caaagccagc agcgcgacct
     4981 gatcctcaaa catccggacc ttgccggcaa gctgcatgcg gccaaacaac tgaccgccga
     5041 ttccaccgcc gagcaggcct ccgccgggct cgatcatctg accgatgccg atcgcaagcg
     5101 gttcaccgaa ctcaacgatg gctacaaggc aaggttcggc tttccctata ttctcgcggt
     5161 caagggcaag accaaggccg aaatcctggc caatttcgaa gagcggctga cgaattccgc
     5221 ccaacaggag tttgctaccg cgctggccca gatcgaacgg atcgcgctgc tgcggctcac
     5281 cgacatgccg ggtcttgggg tgcgcgcatg atccgcctgc cgatccggcc gctgacgcgt
     5341 gaagcttttg ccccgttcgg cacggtcatc gagaccaccg gcgaaggcct gaccatcaac
     5401 gccggttttg cccgtcgctt caatgaccgc tgcaaggtcg atgtggggtg cgaaggcggc
     5461 gagaccaacc tcagcatctt catcgcccgc gcccgcgcgc tgccgctgac catccccatg
     5521 atggaaatgc acccgctcgg cagtcaggcg ttcttgccgc tgcaggatgc gccgtggctg
     5581 gtggtggtct gcaccgatcc ggatgatcca gccagttacc acgcctttgc ctgccagggc
     5641 actcagggcg tcaactatgc tcgcggcacc tggcatttcc cgctgatcac cttcggcgaa
     5701 ggggaccgct tcatcgttgt tgaccgaaag ggaccgggca acaatcttct cgaacatgat
     5761 ctggcagcgc atcgccagcc aattgtcaca gactgagagt cgtcaatgtc acgccgcccc
     5821 tatttcgccc cgcacggtgg cctgccaccg cagacgcagc ttctgaccga ccgggcgatg
     5881 ttcacgccct ggtatggcgt catccccaag ggaaccttct ccgatatcgt cacctcctac
     5941 ctgccgttct gggacaagac ccggctgtgg gtgatcgccc gcccgatgag cggctttgcc
     6001 gagaccttct cgcattacat catggacgtg gcacccggcg gcggctcgac ccggcccgag
     6061 gccgatcccg aggcgcaggg cgtgctgttc gtggtcgagg gcgggttgtg cctgacaatt
     6121 gacgggaagg cgcatgacct tggcgccggc ggctatgcct ttctgccggc cggttgccgg
     6181 tggacgctgg ccaatcccac cagccagccg gtgcggttcc actggatccg caagcgctat
     6241 caggcggtgg aggggctggg ccacccggag gctttcgtca cctccgatca ggccgtcaaa
     6301 cccatcccga tgccggacac caatggcgcc tgggccacca cacgctttgt cgacccgtcc
     6361 gacctgcggc acgacttcca tgtcaacatt gtcaatctgg aacccggtgc ggtcattccc
     6421 ttcgccgaaa cccatgtgat ggagcatggc ctctacgtgc tcgaaggcaa ggcggtctat
     6481 cggctcaatc aggattgggt cgaggtcgag gccggcgatt acatgtggct gcgcgccttc
     6541 tgtccgcaag cctgctacgc tggcgggccg gggcggttcc gctacctgct ctacaaggac
     6601 gtcaaccgcc atatgccgct gggcccgttc cagcagcgct gatcctcaca gcgatttcag
     6661 ccagtcggcc agcgttcgcc gctcctcgcc ggtcatctcg gtgaggttgt ttggcggcat
     6721 cgcatgggtc agcaccgacc acatgcggat ggcgtcggcc tgtagggcga tccgttcggg
     6781 cgtgtccagc atcacgcctt tgggcgcgat ctggatgcca tcccagaccg gctccggcgc
     6841 atggcacatc gagcagcggg agacgatgat gtccgataca tgcgccggcg cgggcctgag
     6901 gccggccagc atcacgttag gtgtctcgcg cggagcaaga cccagccgct ggcgcccccc
     6961 gggcgaggcc gccatcgcca catagaacgc aatgcccaga gcgatggcgg cggcagccca
     7021 ggtccaccac ggcgacttgg catgatcggt atggcggata ttgtagaaat ggcggatcag
     7081 cgcgccggcg acaatgatca ggaccacgat ggccgggatc accgccgcat tggcccagac
     7141 catcgggtag tggttcgaca gcatcatgaa cagcacgggc agcgtgatgt agttgttgtg
     7201 cgcgctgcgc tgcttggcct gcttgccata tttgggatca ggcgtttcgc ccttttccag
     7261 catcgcgatc accttgcgct ggttgggcat gatgatcatc gccacattgg cggtcatcat
     7321 cgtcgccatc agcgctccgg tatggatcag ggcgccacgt ccggaaaaca cctgggtata
     7381 gaaccacgac atcgccacca cgaagccgaa cccagcaagc gccagcagat tgtcattctt
     7441 gccgagcggc gacttgcaga gctggtcata gactacccag ccgagcgcca gcgaaacaag
     7501 tccgatcgca cttgcctgcc acggcttcag cgccatcacg gaaggatcga tcagatacag
     7561 cgtcgcctgg gcgtaataga cccagaccag caggatgaag ccggagatcc atgtccagta
     7621 ggattgccat ttgtgccagg tgatctcggc cggcagggtg tcgggcgcga cgaaatactt
     7681 gcgcatctcg taaaagccgc cgccatgcac ctgccaggtc gccccgccgg ccttgatcga
     7741 ggggtcaggt ttcagcgagg catcaagatg catgaaaaag aatgacgccc cgatccaggc
     7801 gatggcagtg accacatgca gccagcgcag cagcatgctg ccccagtcca tcagatatgc
     7861 ctccatactg ccgcctccct taccctgccg acatcgttat cattagctta gagcgtgctt
     7921 tcggcaatca accggcttcc acacaatcgc agcatactcc aggctgacgc gggtgccgcc
     7981 aggcgttatg ctgacttatc ggacacccta catctgcgga caccatcatg ccacgttttg
     8041 ctgccaacct gtcgatgctg ttcaatgaac atgcgtttct ggatcggttc gaggcagcgg
     8101 cgaaggccgg attcctgggc gtcgagtttc tgtttcccta tgccgaagac aaggccgtgc
     8161 tggccgaaaa gctggcggcc aacggcctga cgcaggtatt gttcaatctg ccgcccggca
     8221 attgggacgc cggagagcgc ggcattgcct gcctgcccga ccgggtcggc gagtttcagg
     8281 acggtgtcgg caaggcgatc gattatgccc gggcgcttgg caaccggcag gtcaattgcc
     8341 tgtccggcat cgcgccggca ggcgtgccgc gcgagcagat gctgcgcacg cttgtcgaaa
     8401 acctgcgatt tgcggccgaa aaactcaagg aagtcggcat cctgctgatc gtcgaaccga
     8461 tcaatttcta cgacatgccc ggctttttcc tcaaccgctc ctccgaggcc gttgcgattt
     8521 tcgatgaggt gggatcggac aacatcaagc tgcaatacga tatctatcac atgcagcgga
     8581 tggagggcga actggccgcc accatcgaaa ggcttctgcc gcgcattgcc catatccaga
     8641 ttgccgacaa tcccggtcgg cacgaaccgg ggaccggcga gatcaactat ccattcctgt
     8701 tcgcgctgct cgatcggctc ggatatcccg gttgggtcag ctgtgaatac cgccccaagg
     8761 cagggacgct tgcggggttg ggctggatgg ccggcaccag ccgggtctga gccaccagca
     8821 cctctatgac gccgttctga tgtctggccg gaacgcggct tgtgggcgat ttgccacatc
     8881 acccgtttgg gtgatgcatt cagccatctc agcggcctaa cctcgtgttc aattactggc
     8941 ttggcggttt ccggcatccg gatttgagca cctaatcata ttttttccct ttgctcagac
     9001 gccgtgaatt ggcgttgcgc atcagaccag ccaggacata cgcattccct taccatcccg
     9061 atttgccagc tgtctcttgt ggccggcaac gaccgcgccc acgagcttgc cgacaggcgc
     9121 atcatgggca tcgaccggtt gagcgacgga caatcagttc gaccggcagg cagatcgagg
     9181 tgttggcgtc ctgatgcctc agcatcgcaa tcagtgtttc cgccgcctcc gcgccgatgc
     9241 gtgccgtggg aacatgaacc gttgtcagcg atggagagac atgcgcggac atgtcgagat
     9301 catcaatgcc gaccaccgaa acatcgttgg gcacggccag gccacgggcc tgtgcctcga
     9361 ccatgcagcc aattgccagc agatccagac cgcagatgat cgccgttggc ggttcagcca
     9421 gttccagcag cttggtacaa cccatccgcc cgccggccag ggtggttggc gcctcgcaga
     9481 tcatcgatgg cggcagcgtc agtcctgcgg cctcgagcgc tctgaggacg ccgagctgac
     9541 gtgccctctg gcgatcattg aagcggatcg cgccggtgaa tacggcgatc cggcgatgtc
     9601 cgagtgcgat gaggtgccgc gcggccatgt ccccggccag ctcgttgtcc accaccaccg
     9661 ccggcgcccc gggcggagcg cgccatgtca gcacaaccgg cacatctgcg gcggccagca
     9721 gggcgctggc ctcgctcgcc cgctcagcac caaccaggat cagcccgtca accccacgcg
     9781 acagcagggt gcggatggcc tcggtctcgg cggcgagatt gtagtcgtgg gaggcaacca
     9841 gcaactggta tccctcggtt gccaggcgcg cctgcatggc ctgcaaggcc cttgagaaga
     9901 tcgcattgtc gagggtgggc atgaccgcgc cgatcgtcag cgagcgcccc gaagccagcg
     9961 cccgcgcagc cgcatccggg cgatagccga gccgttcggc cgagcggcgg acgcgggcga
    10021 gcgtcgcctg cgacagcagc tccggctggc tgatggcgcg cgaaaccgtt gcgggcgata
    10081 ccccggcatc ccgcgccacg tcactcagcc ggatcttgct gcgcttcatc ggaacaggac
    10141 tgaaagaatt tgcatcaacc gacgatagag caatttgtac aatttgcgca atcgctgctg
    10201 aaaattcttt caggaaattg gcgattgact caagatcggc cactcatcac tatgaatgaa
    10261 agcgctttca actatgcctt ggatcaccgg aggggacgtc ggatggcatc ggtcggcagc
    10321 cgcaggacca tgatcggctt ggcaacgctg gctggcctga ttgtcctgtg gtttctcctg
    10381 accacggtga cggggctggt gacgggagcg aggttcccgt caccgtccga attctggaca
    10441 tcgctgacgc agatttccgg gcgcggatat gccggggcga gcctggcgac ccatgcgctc
    10501 cacagtctca aactggtggc gatgggcttc agctttgccg ttctgaccgg cattccgctt
    10561 ggcttgtgga tgggttggag ccgccgggtg gaggctttcg tcaatccgat tttcctgatc
    10621 atccggccga tcccgccgct ggcatggatt ccgctggcga tcctgtggct gggtctgggc
    10681 gatgcggcca aggtcatggt gatctggttc tcggccttcg tgccctcggt catcaatgcc
    10741 ttcaccggcg tgcgcaacat tgaaagaccg gtgatcgagg cgatgcagat gctgggcacg
    10801 ccgcgctggc ggatgatccg ggaagtgatc gttcctgctg cttcaccaat gatttttacc
    10861 gggctcaggc tgtccctgca ggcaagctgg accacgctgg tcgccgccga gcttgtcggc
    10921 gcgctggttg gcgtcggttt tgttctgaac atggcgcagc aggacatcta tcccggcatg
    10981 atcctggtcg gcatgatcac ggtgggcatc ctcggctggg cgacaaccat gctgttgggc
    11041 tgggcggaaa ggcgcgcgct ggcctggaac gttgcggcga gggattgatc catgaccggt
    11101 cgtcccctgt cctcgcttga gaccgtgctg ctgggccttg cggggttgtg tgcttttgtc
    11161 ggcctgtgga cgctgctgtc ggtgaccggc atcgtgccga gacagtttct gccgacgccg
    11221 ctcgatgtgg cgacgcgtat gatccagctg ctgtcctcgc ctttcgccgg cgcgaccttg
    11281 ccgatgcacc tcgcctcgag tttccagcgc tatgcctatg gcgtcgtgct ggcagcgctg
    11341 atcggcgtgc ccctcgggct gctgatgggc tggttccggc tgctcgatga catcgtcacc
    11401 ccgattttcg acgggttgcg tttcatcgcc ccgatcgcct gggtcccgtt cgccgctttg
    11461 tggttcggaa caggcatcgg cggcccggtg atgatcattt ttgccggcgc ttttcctccc
    11521 tgcctgatca atgcctatcg cggcgcgcgg tttgtcgagc cgcgtctgat cgaggccggc
    11581 cgcatgctcg gcacgccgaa tgcgcggatg atccttgagg tgctgctgcc ggcggcggtg
    11641 ccctcgatca tcgccggcct gcgcgtctcg gccggtctcg gctggcagag tctagtcggc
    11701 gccgagttga tcgtcgcggc ggccggtgtc ggcttcatga tggtgcaggc gcaaggctcg
    11761 gtgcagaccg cgaccgtgat ggtcggcatg gtcgccatcg gcctggtcgg catgctgatc
    11821 gacgtgctgc tgcggcaggg tgaaggctgg ctgcgccgtc gtcgcggtct tgaggcatag
    11881 gagaaggcga tgggctcgat cagctttcgc aaggtcgaca aggtcttcgg atcgtccagc
    11941 ggcggcgtca aggcgctcga tgacatcaat ctggaaatcg ccgacaagga gtttgtcgcc
    12001 attgtcgggc cttccggttg cggcaagacg acctgtctgc ggatggtggc ggggtttgag
    12061 gcggcatcat ccggtgaggt tctggtcaat ggcaaggcgg tgacggaacc ggggccagac
    12121 cgtgccgtgg tattccagca gtttgcgctg tttccctgga agaccgttcg ggagaacatc
    12181 gaactcgggc tgcgcaacaa gaacctgcca gcacccaagc gcgatgagct gatcgccggc
    12241 gcgctcaagc tgatgagtct tgaaagccac gccaatgcct tcccgcatca actgtccggc
    12301 ggcatgcagc agcgcgtcgc catcgcccgt gcctatgtgc tcgatccggt cgtgctgctg
    12361 atggatgaac ctttcggcgc gctcgatgcc cagacgcgtg tggtcatgca ggaagaactg
    12421 gtgcggctgg cccgccgcaa tccccgcacg gtgctgttca tcacccatgc ggtggaggag
    12481 gcggtctacc ttgccgaccg tgtggcgatc atgacccgcc gccctgggcg gatcaaggaa
    12541 gtgctcgaca tcaagaccat ccgcgagacc gagaactggg acagtcattc caagatcgag
    12601 gatgtgatgg atcttgaatc cttcgtccat ctgcgcaccc acatctggaa gtcgctgcgc
    12661 gaggaaaaag gcttcaagga tgtctgaacc gcgccgcctc gtccgcgacc taacggagcc
    12721 ggagccgatc cccgaggcag gcatcgcccg cgcggtagaa ctgatgcggt caggccgtct
    12781 gttccgctat ggcgagatgg gcgcggacca gcttgatgtc tcgctgctgg aaacggagtt
    12841 cgcggctttt gccgggcggc gctactgtgt tgccgtcaat tcgggcggtt gcgcgctgtt
    12901 tctcgcgctc aaggttgcag gtgtcgcgcc gggcgatccg gttctggtca acggcttcac
    12961 gcttgccccg gtgccgggcg ccatcctgca tgccgcagca aggccggtga tcgtcgagat
    13021 cggcaccgat tacgtgattg atcccgatga cctgaggcgc aaggcgcagg catcgggcgc
    13081 tcgcgtgctc atgttgtcgc acatgcgcgg ccatattgcc gacatgggcg ctgtgatggc
    13141 tgtggccgag gaatgcggtc tggccgtcat cgaggattgc gcgcatgcga tgtgcgcctc
    13201 ctggcaaggg cgcgccatcg gcacgtttgg cgtcgcggcc gccttttccg cccagaccta
    13261 caaacatctc aacgccgggg aaggtggctt tcttgtgctc gacgatccgg cgatggcggc
    13321 ccgcgccatc ctgcattcgg gcagctacat gctgcatggc cagcatctga gttctcccgg
    13381 cgaggcagcg ctggccgcag cgggcgatca tgtgccgaat ttctcgatgc gcatgacggc
    13441 gctggctgcg gcgatgctgc gtccgcaact caaggaactg ccgcgccgcg ttgcacgctg
    13501 gaatgccatc cacgaccgga tcgccgccgg attgcagcgc agccagcatc tgcatgtgcc
    13561 cgagcggggc ggcgggcagg cctattcggc gacgtccgtg cagttttcag tgtccggcct
    13621 tgaagcggcg gagatcgagc gctttctcgg ccttgccagg acccgcggcg tgccgatcaa
    13681 atggttcggc gccgaacggc agaccggctt taccagtgcg ccgcgccatt ggcgctatgc
    13741 cggcgatcag ggcgcgctcg cgccgaccca tcgggtgctg gccagccttt gtgacatcag
    13801 gacccccgtc acgctgagcg atgaggattg cgatctcatc gccgagatcg tgcgggagtc
    13861 gatcgagacg gttgctgcgg atcgcgtggc atcgaacggc aaacagacga cactgttgcc
    13921 agcatagccg gatgacaacc aacgggagga cttcatgaaa cgcattctga caggattggc
    13981 tctggccacg gccctcgcca ccacaatgcc gctgaccgcc agcgcccaga cggcgatcgg
    14041 cgtcagttat cagccctcgc tctactgggc gctgccgttc cactatgcca acgtcaaggg
    14101 ctggtggaag gatgtcgggc ttgcgcccaa tttcaccacc ttcccggccg gagcgccgca
    14161 gatcgcggcg gcgccgtcaa agtcctggga tgtcggcgga accggctcgg ttcccgccgt
    14221 gctgggcgct gtgcgcttca atatcctgac catcggcatc accaatgatg agtccaagac
    14281 caacgccatg atggtcgtcg gcagcaagtt cgacgcgatc aaggccaatc cggcctcgct
    14341 caagggccag cgcctgttgc tcaccaccaa ctcgaccgtc gactatgcct cgcgcaaatg
    14401 cctggtgaaa atggggttgg ccgcaaatga catgcagttc gtcaatcttg gtcaggcgca
    14461 gatcatcacc gcactgacgt cgaacaacgg cgacatcgcc ggcgtctggg cgcccaacac
    14521 ctacacgctc gaggataacg cgggagccaa atacctctgc tcgggcgccg atgccaatgc
    14581 gatcgtcccc ggcgcgctca tcgttcgcgg cgacttcgcc aaggagcggc cggacgacgt
    14641 ggccaagttc ctggccgtgt atctgcgcgg ctggtcctgg gccaaggcca atccagctga
    14701 agcccgcacg ctggcgctgg acttctacaa acagggcggt ctcgaagtct cggcacgggc
    14761 catggatcag gaatttgcgc tacgcccgac cttcggcctc gacgagcagc tcaagatcct
    14821 cgatcgcagc gcgtccgggt cgacggtcga tggctggttc tcggaaatcg gcaagttcat
    14881 caccgaggtc ggcaccatcc cggcgaaccc cgacgcgaag agctacatca ccaacgaatt
    14941 catgaagaag gtcgcagccg atccgaagct gaaggctttt gccacggagt tcgacaagaa
    15001 gtgaggaaat cgggcgcagc cgcgtccgat gggactgacg ggccgccgca gggcggcccg
    15061 gacgacttga tggatcagga gcaggatatg gccgtcacct atctcaagaa agccgccaag
    15121 acaccggaga ccgagacgga cgctgcgcgc aaggtcgtga ccgagatgct ggcggcgatc
    15181 gaggccgggg gcgaggcggc cgtgaaggat tatgccttca cactcgacag gtggtccggc
    15241 ccgatcgtca tggacgaggc gatgatcgcc gagcgcacca gggacgtgcc gcagtcggtc
    15301 aaggacgaca tccgtctcgc ggccggccag gtacgccgct ttgccgaggc gcagaaagcc
    15361 tcctgcaagg acttttccat cgaactgtcg cctggcctgc agcttggcca gaagatggtg
    15421 ccggtcaata ccgccggctg ctacgtgccg acaggccgct atgcgcatat cgcctcggct
    15481 tacatgtcga ttgccacggc caaggcggcg ggcgtgaaga cggtggtggc ctgttcggct
    15541 ccgttcaagg gcaccggcat ccatccgcat gtgctctatg ccatgacggt ggccggcgcc
    15601 gacgtcatca tgtgcctcgg tggcgtgcag gcgattgcgg cgatgaccta tggccttttc
    15661 accggcaagc ctgctgacat catcgtcggc ccgggcaaca aattcgtggc ggaagccaag
    15721 cgcatgctgt tcggcaaggt gggcatcgat gtgttcgccg gcccgtccga ggttgccgtt
    15781 ctggccgatg acagcgccga cccgctgatc gtcgccaccg atctggttgg ccaggccgag
    15841 catggccacg aaagcccggc ctggctgatc accgattcgc gccgcatcgc cgatgaggtc
    15901 tcggcactgg tgcccaagct gatcgccaca ttgccgccga ccgcgcgcga tgctgcgggg
    15961 gcggcatggc gcgactatgg agaggtcgtg ctgtgcggca gccgcgaaga aatggtacgc
    16021 gtgtcggacg cctatgcctg cgagcatctg gaggtgcatt gcaccgatct cgactggtgg
    16081 catgccaacc tgaccaatta cggttcgctc tttcttggcg aggagaccaa cgtggcgttt
    16141 ggcgacaagg tctccggccc caaccacatc cttccgacca aatttgcggc gcgctattcg
    16201 gccggccttt cggtgcacaa gttcctcaaa ccgctgacct ggcagaagat gagccgagat
    16261 gcctgcgcca cgctggcgcc ggtctcggcc cgcatttcgc ggcttgaagg gatggaggcc
    16321 catgcccgca cctccgatgt gcgcatggcc aaatatgcgc ctggaatgca gatcgaactc
    16381 ggccggccgg tggagtcctg agtgtcgttc cgcctgacgc atctgttcga cctttccggt
    16441 cgcaaggcgc tggtcaccgg cggcaacagc ggcattggtc tggccatggc gcgggcactc
    16501 gggctggcag gcgcaaaggt cattctcgtt gcccgtcgcg aggcagagct ggcgcaggcc
    16561 gcaaccggcc tgcggcagga tggaatcgat gccgtcgccg ttgctgcgga tcttgccgat
    16621 gccgcagtcg gtccctcgct cgcgtcctgc tgcggcaccg acatcgacat tctggtcaat
    16681 gcggccgggg tgaacctgcg ccagcccttt gccgaggtga cggcagcggc gttcgatctg
    16741 catatggcgc tgcatctgcg cgcgcccttc ctgctcaccc aggcttttgc gcccgccatg
    16801 gccgcgcggc gctggggccg catcatcaac ctcgcctcct tgcagagcag ccgtgccttt
    16861 cccaacagcg cgccctatgg cgccggcaag ggcggcatcg tgcaattgac ccgcgccatc
    16921 gccgaggaat ggtcacgcca tggggtgacg tgtaacgcca tcgctcctgg gtttttccca
    16981 acgccgctga cggcgccggt gttcggcgac cccgagcgcg ctgcgcgcaa cgcagcccag
    17041 acggccatcg gtcgcaacgg agatctggac gatctgaccg gtgccacggt attcctggcc
    17101 tcggatgccg cagcctatgt caccgggcag acgctggccg tcgatggtgg ctttaccgcg
    17161 aaataggaac tttccatgaa ggcgcttgtc tacaccggcc ccaacagtct ggcctatcgc
    17221 gacgaacccg atcccgttcc gcaagcgggc gaggttttgg tgcggatcga ggctgttggc
    17281 atctgcggct cggacatgca tgcctatcac gggcatgaca gccgcaggcc ggcgccgttg
    17341 atcctcggcc atgaagcggc cggccgcatc gtcgggggcg cacgggacgg cgagcgcgtg
    17401 acgatcaacc cgctggttgt cgatcccgcc tgcccctatg cgatcgaggg gcgctggcat
    17461 ctttccccga cacggcagat catctccatg ccgccccgcc cgggggcttt tgccgaatat
    17521 gtcagggttc cggcgccgaa tgcggtggcc gttcctgcga ccatgccgat cacacatgcg
    17581 gcattggcag agccggtggc tgtctcgtgg cacgcggtcc ggattggcat gcagcagctg
    17641 catcagccac tggcctgctg ccgcgtagtg gtgctgggtg gcggcgccat cggtcttgcc
    17701 gcagccatcg ttgcccgtca ttttggcgca aaggacatcc gtatcgccga gaccaacccg
    17761 ctcagaaccc gcctgatcga gaccgaggaa gggtttacgg tctatgcgcc cggctcagcc
    17821 ggagcgcccg ccgacaacac cattgacctt gtgatcgacg cggtgggcgc caatgccacg
    17881 cgcgccgctg cctcggcgat ggtcagaccc ggcggggtca tcgtccatgc cggtctgctg
    17941 ccgggtgccg agggcctcga tatccgcaag atcaccttgc aggagatcat tgtcaccgga
    18001 acctattgtt ataccccggt cgattttcat cagaccatcg ccgctctggc ctcgggagcg
    18061 ctcggatcgc tgacatggtt tgcggagcgg ccgctggccg acggggcgca ggccttccgc
    18121 gatattgatg ccggtgtcgc cagcgcgccc aagatcgttc ttaggtgctg atcgcaaatc
    18181 cagagcgctt tcagttaatt ccagttcacg taaatgtgct ctatcttctt gttttatcgc
    18241 attttcttaa cgcgaaccgg aatccacttc gcttgaaaat gctctagccc ggttcagcac
    18301 catgcccgac cggcataatc cagcagatag cgctccgggg cgcgggccag cgcctccagg
    18361 ccgatcagcc ccggctcctg cgccgtcacc agctgcaccg gtatccggcc gagcaactcg
    18421 gcatgggcgc tgttgtcctc gaatgccgcg cgaaaagccg ccgcatcgat cagcgaccgg
    18481 aggcgcggca ggatcccgcc agccagaaaa acgccacctg tcgccagaaa ggtcagcgcc
    18541 atgcccgcac agaacctggc gagtagcgtc agccacaggt cgatcacctg ccggcacgcc
    18601 gggtctcctt ccagtgccgc ctcgacaatc gccggcggcc ttgccgcgtc cggagggcag
    18661 gcgggatcga acgccagccg caaccgggtc agtgccggac ccgccagcag gctttcggcc
    18721 gacaggctgc gcccatcggc cgccagacgg gcaaacaggc ggtgttcggc aggcgagccg
    18781 gcggccagcg ccgcatgacc accctcggac gagaccggca tgagagtctg gccagccctg
    18841 atcagggcgc cgaccccgag accggtgccg gggccgacga tgagccttgt gccgtgcccc
    18901 gaaaggccag ctccgatcgg catgaggtca tccgatgcca gcgcgggcag ggccagtgcc
    18961 agcgtctcga aatcattgag cagcaacccc tgccgcaagc cgagccgttg cgccaacgcc
    19021 ggcccgtcga tcacccaggc ggcattggtc agccgcaccg agcggccgct gatcgccccg
    19081 gccgcaccga gcagcaggct ggccggcatc ggccagccgg ccttgcgaca agccatctgc
    19141 acggctgcgg cacaatcggg cacgccgcca gtctcaaagc gcagacagtc cgacagcggt
    19201 gcattcgccc gcgccagcat ggcaaagcgc acattggtgc cgccgatgtc ggccagcagc
    19261 gccgggaagg cgacccgcat acgcctattt tccggtcttc acccgcgtcc actgccgcgt
    19321 caccaccttc tggacccgct cgtcataggc ggtgttggtg aacaggcgcg cgaatgtcgc
    19381 ggcatcgggg tagatattgg ggttgtcgag gatctccttc ttgatctcgg ccttggccgc
    19441 gagattgccg gaggcatagg agaccatgtt ggagttgcgg gccgccacat cgggccgcat
    19501 catgaaatcg agaaaggcgt aggcattgcc cttgtttggc gcgtcggccg gaatgacgaa
    19561 ggaatcgaac cacatcagcg ccccttcctt ggggatcagg tagtcgatct tgacgccatt
    19621 gttggcctct tcggcgcggc gcctggcctg cagcacatcg cccgaatagc cgagcgcaac
    19681 gcagatgtcg ccggcagcaa gcgcattgat atactcggag gaatgaaact ttctcacagc
    19741 cccgcgcacc ttcatcaggt gatcggcagc cttggtgata tcctcgggct tctttgaggc
    19801 cggttccagc ccgagatagc gcagcacacc ggggaacacg tcttcggggg cgtcgagcat
    19861 gtagacgccg caatccttca acttttcgac cacctgcggc ttcagcacca gatcccatga
    19921 gttgagcggc atatcgccca gccgctcctt gacctttgca acgttgatgc cgatgccggt
    19981 cgtgccccag agataattga ccgcgtagac attgccggga tcgtagacgg ccagcttctg
    20041 ccggacttca gcccacatgt gcttgaggtt cggcagccgc gcctgatcca gcttctcgaa
    20101 gaccttggct tgaatcagcc gctgcaggaa cgggcccgag ggcacaacga tgtcatagcc
    20161 ggacttgccg gtcagcatct tggtctcgac aatctcgttg ttgtcgtagg tgtcatagac
    20221 gaccttgatg ccggtctcgc gggtgaaatc ctcgagcacc ttcggatcga tatagtcgtt
    20281 ccagttgtag acgttgaccg ccctgtcctg tgcccaggca acggacgagg ccagcaccgc
    20341 cgcaaggccg atcaggcgaa ccgacatgaa ttcctcccat gcttcaaatc tgacatgctc
    20401 tggtgttttg gtaaggtcta tgcgtcggca gcgcaagcgg ccgggctggg tttagcgttt
    20461 gagccttgcc agccggtcca gtgcggcatc aagcgtggta tctgccttgg caaaacagaa
    20521 gcggacaacg ctgcgcacgg catcctcggc atagaaggcc gaaacgggga tcgccgccac
    20581 cccttcctgc acaaccaggc gctgacagaa cgcggcatcg tcagtctcac ccaggctgga
    20641 gatgtcgaca ttgacgaaat aggtcccggt cgaggtcagg acgtcaaagc cgagcgccgt
    20701 cagcccggcg acgaaacggt cacggcttcg ctgaaagccc tgccgcatgc cggcaaaata
    20761 actgtcctcc ttgtcgagcc cataggcgac ggccgcctgc agattgggcg gcgtggtgaa
    20821 ggtgagaaac tgatgggctt tcgacaagac ctgcatcagc ggtggcgccg ccagcacaaa
    20881 gccgaccttc cagccagtca gggaaaagat cttgccggcc gagccgatct tgacggtgcg
    20941 ctcggccatg ccgggcaggc cgatcaacgg cacatgatgc ttgccgtcga agatgacatg
    21001 ctcccagacc tcgtcgcaga ccgcgatcgc gccatggcgg acgcagaagc gggccagcaa
    21061 ttcgaggtcc tcccggtcat agacgacgcc ggccggattg agcggattgt tgaacaggac
    21121 aacacgcgtc cgatcggtga aggccgcggc aaggtccgat tcggtgaagc gccagtgcgg
    21181 tggccgcagc ttgacgaagc gcggcacacc gccggcacgg cgcaccagcg gcagataggc
    21241 atcatacatc ggctggaaca gcaccacctc gtcgcccggc tcgatcagcg ccagcagggc
    21301 tccggcaatc gcctcggtcg cgccggaggt aatcatcacc tcgcgctccg gatcgaccgt
    21361 gatcccctga aaccgctggt agtggcgggc aacggcctta cgcagttccg gcaggccgaa
    21421 catcggcgga tactggttcc agccatgcac cacggcttcg gcggccttct gccgcacatc
    21481 gagcggccca ggatcatcgg ggaagccttg cccgaggttg acggcctgat gctcgcgcgc
    21541 cagccgggac atcgtatcga aaatggtggt gggcaatcgg gaaaagacgc tgttcacggg
    21601 ggctgctctc cttgcaaact gcatagacca gcaggacgct gcgtaaaagt gcccggatgc
    21661 gcgcaggctg ttgcggctgt ccgtgttggt gtcgccggag cggatttcgc cagcctgccg
    21721 ttgccgggcg gcgttcggcc tcgcgcggcc aggaaaactg gactatgaaa ggtttccagc
    21781 ctgtcgttca ggatttgaat ccgtgctctg gacgatcacc gggtctaagt gtctcggacg
    21841 tgtgccccgg catgaatgac agcagcatga ttttgcagcg ccaggaggtc tgagccatgc
    21901 gtgtcgacgg ccaacgtctc tggcgccggc ttgaagccat ggcggccatc accgagcccg
    21961 accgtcccta tacgcggcgt tccttcacgc cgcggtttat cgaggggcga gcctggatcg
    22021 ctgatgcgtt ccgcggggcg gggctggtga cgcagattga tgctggcggc aatctgatag
    22081 gccggcttgg cggcagcgac aacaacctca agccgattgt catcgggtcg cattccgata
    22141 ccgtgccgtc cggcggtcgc tatgacggtg ttctgggcgt tctggcggcg cttgaggtgg
    22201 ccgaagccct gcaggcagca ggcaagcggc tgaggcaccc gctggaagtc gtcgatttcc
    22261 tagccgagga gccttcggaa ttcggactgt cctgcatcgg cagccgggcc tgggccggag
    22321 ccctctcccc cgccgatcta gatcgccggc gccctgatgg catgaccctg gcggaggcga
    22381 ttgcctatgt tggcggcgat ccgtcgcgtc tggcgggtgt caggcgagcc aaggggggca
    22441 ccgccgccta tctcgaggtc catatcgaac agggcatcgt gcttgaacgg tcaggcacgc
    22501 cgatcggcat tgttaccgac attgtcggca ttcgccgcga gcgctggatg atttccggtc
    22561 gcgccgacca tgccggcgcc acaccgatga acctgcgcaa ggatgcgctt gtcggcgctg
    22621 ctcatgtgat cggggaagcc tccgccgccg ctacggcaac gctgggaacg gccaagcctc
    22681 tggttgccac aatcggccac atcgaagtcg aaccgaatgc cgccaatgcg gttccaggca
    22741 gggtggtcat gacgctggag gttcgctccg gcgatgatgc tgcagtccgc cggttcggtg
    22801 acacgctggt agagcggaca aaggaccgcc ttgccgctct cggccttgag ctggcgcgaa
    22861 gcgagatcag ccacgccttg ccaaccccct gtgccgatgt gatccggtcc gcaatccggc
    22921 atgcggcaca tgatcttgga ctggcgacgg tggatttgcc cagcggtgcc ggccatgatg
    22981 gtgttttcgt cgcggcaacc gggccggtgg gcatgatctt caccccttgc gaggggggac
    23041 gtagccatgc tccggaagaa gcgatgacac ccgagcaggc cgaatccggg gcagctgcct
    23101 tactggctgc ggtgctcagg ctcgacgaga cgttgccaac cgacagcctg ccgacctgaa
    23161 atccggtttg gaataaccgg gcttggagca ttttcaagcg aaccggactc cgcccttcct
    23221 gaaaacgccc cagggtcagg cgcccgccta ctgacctccg aagaagtcct tcttcatacg
    23281 ggcaaagaag ccttcggatt ccgggtgcgt gccgtcggcg gacccctgtt cgaactctcg
    23341 cagcagttcc ttctggcggc gggtgagatt ctgcggcgtt tccacaaccg tctggatgaa
    23401 cagatcgccc cggtctttgg atcgcagcac cggcatcccc ttgcccttca cccggaactg
    23461 cttgcccgac tgggtgcctt cgggaatcct caccttcgca tcggtgccgt caagtgtcgg
    23521 caccgcgatc tcggttccga gcgcggcagc gaccatcgag atcggcaccc gggcgaagag
    23581 atcggcgccg tcgcgctgga aaaggccgtg cggcttgacc gacaggaata cgtagagatc
    23641 gccggacggg ccgccgcgca ggccggcttc gccctcgcct gacaggcgaa tacgggtgcc
    23701 atcctcgacg ccggccggaa cgttgatcga cagggtgcgt tcgcgggtga cccgaccgcc
    23761 accgttgcag gcacgacagg gatcgtcaat ggtctcgccg cgcccgtggc aggcgtggca
    23821 ggtgcgttcg atcgagaaga acccctgcgt cgcccgcacc cggccgtgac caccgcaggt
    23881 ggcgcagcgc ttgggcttgg agccggcctt ggcgcccgag cctgaacagg cttcgcaggt
    23941 gatcgaggtc ggcaccttga tggtcgccgc tttgccgcgg aaagcatcat caagcgtgat
    24001 ttccagattg tagcgcagat cggcgccgcg ttcgcgcccg ccggaggagc ggcgcccgcc
    24061 gccgaagacc tctccgaaca gatcctcgaa tatgtcgccc atgcctgaga acccgccacc
    24121 accgccaccg aacggtccct gcccaccgcc gccctgctcg aaagcagcat ggccgaagcg
    24181 atcataggcg gcgcgcttct ggctgtcgga caggcattga taggcttcgt tgagttcctt
    24241 gaacttggct tccgccgcct tgtcgccggg gttcttgtcc gggtgatact gcatggcgag
    24301 cttgcggaaa gcgctcttca gcgcggcctc gtcagcattc ttctgaacgc cgagaacctc
    24361 gtaatagtcg cgttttggca ttgcgatgat gatccctcat cgcggcgcgc gggccgggga
    24421 agcagagtgt tacgcactaa tggggattat gacgcggatt cgcaagacgc aacctacccg
    24481 gtcggcgcat cttgaccgcc caaccggcga agaattgcca gggcatcgct ggtcatggac
    24541 tgcacgatcg ctgccgcagg ctgtatgtcg gctatcaatc ccgctgcctc gccaaaccag
    24601 acgccggtgt tgtcagggtc tccctcgaca aaagcctggc ggtagcgcgg cgcctcggcg
    24661 gcaattgcat cggcaagggc agcttcgtgc ccatgccatc gactggtgaa cgcgttgcgt
    24721 cgtacccgag cggtaaagcc gggcggccag gctatctgtc gtgcaatatc gaccaccgag
    24781 gtgcggatgg tttcatcgcc cgtcgcctcg acaatggcat ggtgatggcg gggatggacc
    24841 agtgcctcgc gcgaggccca gaatcgcgtg ccgacaagca cgccctctgc ccccagggca
    24901 agcgccgcag caagcccgcg gccgtcggcg ataccgccgg ctgcgacgag aatcgcctca
    24961 ggagcctcag cccggatgag atccgccgct tccgggacaa aggaaacgag gccgcgcagg
    25021 gcgccgtgac cacccgcctc ggtgccctgc gcgacaataa cgtctgcctt gatgtcgatc
    25081 gcgtcccaga catggtcgag cgactggcat tggcagatga tcgccgcgcc agcggcgcga
    25141 atctcgtcga caaaggggcg cgggtcgccg aacgacagca tcaccgcttt cgggcgatgc
    25201 ctgagaacag ccgtcagcaa ggcgggcgat ttgcgcaagg accaggtgat gaagccaacg
    25261 ccgatggctg acggccctgc cagtcgccat tgttcgtcaa gccaggtatc atctccataa
    25321 ccgccgccga tcagcccaag tccgcccgcc ccggtgacgg cagacgccag cgcaccgcca
    25381 ccggcaaagg ccatcggcgc cgacaggatg ggggttcgaa tcccgaatcg ctctgtcagc
    25441 cgggttgaaa gtgagacagg ggcacattca ctcatcgcgg ttctctctgc catgccgggc
    25501 ttggcacaac aagggccgca gcttgcttgc tgcggccctc agttccggcg cgatggaggc
    25561 gatcaggcgc ccttcttgtc gtctttcttc acttccttga agtcggcgtc gatgacatcg
    25621 tccttggcgg cgtgcttggc ggcatcggca tgggcggcct cttcctggct ggccttgtac
    25681 atcgcctcgc caagcttcat ggaggcctgc atcaggcctt ccgtcctggc cgcgatcacc
    25741 tcggcatcat cgcccgaaac aacctcgcgc agttcggaca ccgccttttc gatcgcctcc
    25801 ttttcggctg cgcctacctt ttcgccatgc tccttcagcg ccttttccgc cgaatggatc
    25861 atcgcctcgc cctggttcct ggcctcgacg agttctcggc gcttcttgtc ttcggcggca
    25921 ttggcttcag cgtccttgac catcttttcg atgtcggcat ccgacaggcc gcccgaggcc
    25981 tggatgcgga tctgctgctc cttgccggtg gccttgtcct tggcggtcac cgagacgatg
    26041 ccgttggcgt cgatgtcgaa ggtgacctcg atctgcggca tgccgcgcgg ggccggcggg
    26101 atgccgtcga gatcaaagcg gccgagcgac ttgttgtcgc gcgccatctc gcgttcgccc
    26161 tggaacacgt tgatggtcac ggcctgctgg ttgtcctcgg cagtggagaa cacctggctc
    26221 ttcttggtcg ggatcgtcgt gttgcggtcg atcaggcggg tgaatacgcc gcccagcgtc
    26281 tcgatgccca gcgacagcgg ggtcacatcg agcagcagca cgtccttgac atcgccctgc
    26341 agcacgccgg cctgcactgc cgcgccgatc gccaccacct cgtcggggtt gacgcccttg
    26401 tgcggctcct tgccgaagaa ctgtttgacg acgtcctgca ccttgggcat gcgggtcatg
    26461 ccgccgacga gcacgacctc gtcgatctgc cctgcgctga gcccggcatc cttcagcgcc
    26521 ttcctgcacg gctctaccgt gcgctggatg aggtcatcga cgaggctctc gaacttggcg
    26581 cgggtgagct tcagcgtcag atgcttgggg cccgacgcat cggcggtaat gtagggcagg
    26641 ttgatttcgg tctgggccga ggagctgagt tcgatcttgg ccttttccgc cgcttccttg
    26701 aggcgctgga gggcgagctt gtccttcttc aggtcgatgc cctgctcctt cctgaactcg
    26761 tcgacaagat attcgacgag gcgcatgtcg aaatcctcgc caccgaggaa ggtgtcgccg
    26821 ttggtggatt tgacctcgaa cacgccgtcg ccgatctcga ggatcgacac gtcgaaggtg
    26881 ccgccgccga ggtcatagac cgcgatggtg ccggccttct tcttgtcgag gccataggcg
    26941 agcgccgccg ccgtcggctc gttgatgatg cgcagaactt caaggccggc gatcttgccg
    27001 gcgtccttgg tggcctgacg ctgggcgtcg ttgaaatagg ccggaacggt gatgacggcc
    27061 tgcgtcacag gctggccaag atgggcttcc gccgtctcct tcatcttcat cagcgtgaag
    27121 gcggaaatct gcgagggcga atattgcttg ccgtccgccg tcacccaggc atcgccgttg
    27181 ccggccttga tgatcttgta ggggacgagg cccatgtcct tctgcgtcat cggatcgttg
    27241 aaggtgcggc cgatgagtcg cttgatggcg aagaaggtgc gctcggggtt ggtcaccgcc
    27301 tggcgcttgg ccggctggcc gacaaggcgc tcgccgtcgt cggtgaaggc gacgatcgag
    27361 ggcgtggtgc gcgcgccttc cgagttttcg ataaccttcg gcgtcgagcc gtccatcacg
    27421 gcgacgcagg aattggtggt gccgaggtcg atgccaatca ctttacccat ggcggtgatc
    27481 ccttgttgaa gcggaaaccc tgccggaccc gaagcatccg gctgtcgagg gcggatatag
    27541 gaagggggcc gtttgcgcgc aagagcggag gcggcgggat ttcgcctttc acagcgaagc
    27601 agcgggcgcg ttgtcaccgt ctatggccaa tgataaaatt cggaatgccg gagacgcgac
    27661 cgtgatccct ccttccgtga tcgtcacttc tgttgtggcc attgccggca cgctgtggct
    27721 cggcagtctg gtcctcaagt ccatgacggc gaaaagaaag atagccgaca cccgttatgg
    27781 gcgacggtcc gatggcactg tgacctcgat gtccgacggc agcagcggcg agggtggccg
    27841 cagcgcaagt tccagcgaca gtgattcgtg tagcggggac agtggcggcg acggcggcgg
    27901 gtgtggcgac tgatccagcc tcccgcagct caggccaaca ctttctcaag catagcgtgc
    27961 aaatccgcgc ggtaggcccc gacgagtttg ctataaggct tttttgccaa cgtttcaggg
    28021 gtgagaacgt gtctggcttt gtcgttctgg ggctggtgct gtttcttggc ggcacggcca
    28081 tggctcttgt ccgcatgaac cggctgagct gcatgccctg caattcggct gacgattcgg
    28141 cctattgcaa tccgccgcgg ccgcatcggc cggcgtagac agtcaatcct cctctcctcg
    28201 cccttcgcgg cgggcgccgt agaagtcaat gtagcgcggg gcgatatcgg cgacgcggac
    28261 aatgaccagc acatcggtcg taccgaacgc gcgatccacc acagcgccat cgccgaaccg
    28321 gccgccgagc ctgaggtagg ccttgaccag cggtggcaac acgccgagcg ccgcgcggga
    28381 ctccaccgcc tccgctggca ggcggttcat cggcacatag cgatccggaa gggccgcgac
    28441 ctgccaggca tcgggagcgc gggcatgatg atgcagaaaa gacagcggca gggccagtgc
    28501 agcgggatcc gtgcccggca ggctggcgca gccgatcatc atgtccatgc gatgatggcg
    28561 gacataggcc cagataccgg cccacagcaa ctcgacgatc cgcttggtgc ggtagggctt
    28621 gagcacacag gaacggccga gttcgagaac gcgggcgccg ggatgggcgc gcatcagccc
    28681 gcgcaggtca tattcctgtg ccgaatagaa cccgccgtgc gcgtcggcca catcgccgcg
    28741 cagcaaccgg taggtgccga caacctttgg ccgggcggag cggccggaca gcccctgccc
    28801 gtcatggtca atgaccatca gatgatcaca gatgcggtcg aaccggtcgg catcacggcg
    28861 ggcaagaagc atgcgcgcgg tcggcaccgc ctgacattca ttgaaaaaca cttcgtagcg
    28921 cagccgctga accgcgcgca ggtcgccggc atcctgtgcc agacgaacct cgagcgagcc
    28981 cagccggcaa agactgcccg caggcagtgg cgcggaatgg ccaaaacgat gcggcgtcga
    29041 tggaccggcc aggcgcggcg tccaccgttt cagcagacga agtgcaggag gagacgggaa
    29101 tgccggggat cggttcggaa gcatggcttg gccgcctctc cgtggaatct gggccgggga
    29161 tcgacccaaa gcacgatagg gatacggcgg gccgatgaca aacgtgtgac gttcacgcgg
    29221 ccttatgagg gtttttcagg acatcggcga gggtctgttc aaacaccgcc cggtcaagcg
    29281 gcttggacag gaaggcatca aagccggaag caaggcaggc cgcccgatcc tcggcgaagg
    29341 cgttggccgt cagggcaatg accggcaggt ggggcgctcc ccccatgctc tcgcgttggc
    29401 gcagttttct ggtcaggtcg atcccgtcca accccggcat gcggacatcg aacagggcaa
    29461 gatcgaacat tccctcatcg ggagcctcaa gccgggccag agcctcaagc ccatccttca
    29521 cccagacgac atcgccgcca agcctctcga tcagggtgcg cgccagaagc gcattgaccg
    29581 ggttgtcctc ggccagcagg agccgccggc cggcgagagg cttggccggg gttgcctgca
    29641 gctcgtcccc ggcagccggc tcaggaccat tatccgacgc aaagcgggcg atcagcgaac
    29701 gggcccgcac cggcttgacc agatagccat caaaccctgc gtcggcggga gcgccgaagc
    29761 tgcgtcgctc gaacggcgac agcaggacga tacggcggga gaccccggca gcggcagcgg
    29821 cgtcggtcaa gcgccggcag gcgtcagagc ccagcgccgc gtccaccacc aggacatcgg
    29881 catctgccag acaggaaagc ccctgcccgg tggtcgtcgc aagccgcggc cgcgcaccaa
    29941 gccgggaaag ccgttcggcc agaaacggcg cctcgaacgg agacgccgag acaatgacca
    30001 cgcgctggcc ggcaagcacc gcattcggca ccgccacctc tgcggctgct tcgctggcgg
    30061 caggcaatgg cagggcaaca cggaaaatcg aaccggcacc ggggcggctg atcagcgcta
    30121 tcgttccacc gagatgggtg acgatccgcc gggaaatcgc cagtccgagc cccgacccgc
    30181 catgacggcg accggtatcg gaatcggcct gttcaaaatc ctcaaaaacg gcctgttgac
    30241 gctcgatcgc aatgccggga ccggtatcgg caacggtaaa ggcaatcatg ccaccctgct
    30301 ggctctcgac cctgaggccg acgccaccgc gttcggtgaa cttgaccgca ttgccggcca
    30361 gattggtcaa cacctgccgc aatcgtgccg catcacccag cacaacgggt gggacgtccg
    30421 ggctgacaag ggtggcgatc tcgatgcccc tatcctgcgc acgcggggcc agaagctcca
    30481 cgacgctttc aaccagagga gcaatggcaa cacgctcctg cacgcgctcg agcctgccgg
    30541 cgccgaggcg ggcataatcg aggatctcgt tgatcagcga caacagcgcc tcgccggatg
    30601 agcggatcgc atcaacgtag ttgcgctgtt cgagcgtcag cgccgtatca tccagaagcc
    30661 ccgccatgcc gatgacgcca ttgagcggtg tgcgcatctc gtggctgatg gttgcaagga
    30721 agcgggactt ggcgccgttc tgatcctcca tctgaggctt ggccgcaacc ggttggccaa
    30781 tggcgacgca tctctgcgca ccctggccgg ctcgtgccat gccaatggtc cagcgaacgg
    30841 agacgccggc cagaacgctg tcaatcggac cgttccggtc acggctgcgg ccagcttccc
    30901 caacgagatc gaaacgcaac ggatcgagcc catgccggat gcagaaatcg tgataagctt
    30961 ggttgctgtc gcggatggtc ccgtccgtgt cgcagatgat caggaaagcg ccggcgctct
    31021 ccaccgccga actggccgct ttcagccttg cttctgcctc ggcgcgtgcg cagtcaagtt
    31081 cggaaacatg ccgcatgatc gagcgcgccc gcttgcgcag ccgcgccata tcccgaatga
    31141 gaaagattgc agtgccggtc aacagacccg cgacagccag cgcaacaatg gcaacggcca
    31201 tccaggttgc cgtgctcaga aacagaccat ccgcgttcat cgcttgcccc tcgtcgaacg
    31261 aggatgccgc accaagcgtt gcagattggt ggacagacga gcggttcacg tccggcaccg
    31321 gcaagccagt gtccagcgtg gttaagcgag cg
//
LOCUS       DAOMXG010000008        30350 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_998_length_30350_cov_9.697904, whole genome shotgun sequence.
ACCESSION   DAOMXG010000008 DAOMXG010000000
VERSION     DAOMXG010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 30350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30350
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_998_length_30350_cov_9.697904"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="PLQ11_01160"
     CDS             complement(<1..863)
                     /locus_tag="PLQ11_01160"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HRK23540.1"
                     /translation="MYGMVRASLLVLVAAGFSAAAVAETVGTAGAVSPTAKARGAGAA
                     RVLNVGSSVLYGETLTTGPNGALQVLFVDRTTMTIGPNSQVVIDEFVFDRVSNSGRMA
                     ATLTRGALRFVGGQTSHTGGATLRTPVTTLGVRGGVASVRHDGASGTRVVNHFGRISV
                     AGPEGETMLRRSGFAMGVGGTAAGSGPARSTRAEVDSFADALAPPRVAAKMSAQRSAT
                     RAAAQAAAAQADAAGVTSIQSANASSAAARHSVAGMPGQASPSRQAGDAGSRAGAARQ
                     AALLPSIAPPEA"
     gene            complement(874..2199)
                     /locus_tag="PLQ11_01165"
     CDS             complement(874..2199)
                     /locus_tag="PLQ11_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRK23541.1"
                     /translation="MRHRLWFGLAMWLAAGSAAAQLAPISDDPQLRADHDALFARVLK
                     APADAALSRRYAELAARMGDFEAGIGALERVVLAHPDDWAAHLAIGEMYVRLGALELA
                     RSAFERASAVEAPEATRARAAAFLIEIERRLQPTGWSFSGQTGLRWQSNANAAPSSPL
                     VRALGFDTSLPNTSLRKPDWNAFVQGSLRLVHDLENQRGDTIEAQLTGYYARQFRLAS
                     LNAGVVEANLGPRLALEPDLLPGWSVRPYLLASGVTLGDAPFAHAVGAGVTLAIPLGA
                     ILVEPGYEMHRRIFHTSSGMPTAAESNGRWHNGFVTVSGTLPPGLRWFARLQASRAEA
                     RADRQAHRAHGFDAGVAYGFAVPSLGRKWTLAPFVGWTHTRYDAPDPLVDPLRRRRDR
                     EWRLGVSLDAPVSDRLGFGITVQLVNNRSTLPNYARRNVSVSFGPTLRF"
     gene            complement(2454..3281)
                     /locus_tag="PLQ11_01170"
     CDS             complement(2454..3281)
                     /locus_tag="PLQ11_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009492523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRK23542.1"
                     /translation="MLGLPLADLLLFAAALLGAGMITGLLAGLFGVGGGAVIVPVLYQ
                     IFGIVGVPEEARMHLCVGTSLAIIIPTSIQSFRTHLAKGKTLMDVLKIWALPVIAGVA
                     LGAVIAAYAPGKVLKAVFVVVATINAIKLLAGRDDWRLGDQLPGRAGLASFGFVIGLA
                     SSLMGIGGGVIANMFLTLFGVSMHAAVATSAGLGVLISIPGALGYVLAGWSQMAVLPP
                     LSLGFISVLGFLLVAPTSTFLAPYGARLAHATPKRRLEIGFGCFLLLTAARFVWSMV"
     gene            3387..4619
                     /locus_tag="PLQ11_01175"
     CDS             3387..4619
                     /locus_tag="PLQ11_01175"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRK23543.1"
                     /translation="MPSTAAHAPFNRVTWVAIGGLGITQIIGWGSTYYLLTLLGRPIA
                     TELGLSQAFVSAGLSLMLIMGAVFGPVAGRAMDQRGARPVMALGSLIASAGLVALAFV
                     RGPVGYMAAWFVIGLSAALILYPAAFTALTQIAPHQARRAISWLTLPGGLASTVFWPL
                     TEALLTIMDWRSICLFYAALNLCVCLPLHLAVIRRDPAAADQQDESGPLPQGLPADAR
                     RMAFLLFAAALALNSLLVTGLLNHFIAIMRDFGQPAQAAVAIGMVFGLSQTSARILEL
                     ISGARTDPLMLGVAVAAGFPLAFAAMMVAPASLVAGMAFAVILGMCNGLWTIVRGAMV
                     LRLFGVAGYGENLGTIIVAQGLAAGVAPILLASLMEIAGVYAAFVACGLVSATALVAM
                     VLLFRHARRVEAAARPGP"
     gene            4616..6010
                     /locus_tag="PLQ11_01180"
     CDS             4616..6010
                     /locus_tag="PLQ11_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-efflux pump"
                     /protein_id="HRK23544.1"
                     /translation="MTDTARQTNAARKEWAARISVFASACIALLKLAAGLVSGSLALL
                     SEAGHALIDLGATVLTWFAVKTGDKPADDEHHYGHGKIEAVAALAETGLLIALACYVL
                     YEAIHRLRFGGEVPEITWPVLAVLVVSILVDFNRWMHLRKVAKETHSVALAADALHFS
                     SDLVSSLMVLVGLGAVMAGFPKGDALAAIAVAIFILVAAFELGKRTVNTLIDAAPHGA
                     QDQVVGILREITGVIDVRSVKVRPAGSTLFVEALVSVPRLLPLDRVQQIREAAADAIT
                     AAMPEAEATITVEPKAMDDESVLERVILIAARRRIPVHHVTVQTIGKRLSVSLDVEVD
                     GRMSLAAAHQIATRLEAAIRAEFGPDTEVETHIEPLEAQGLVGIDVDEDERIELARMI
                     LDCAKQIDGLTHVHDLRVRRTDKGLVVTLHLRADPQASVADVHESVDRLEHLARAARP
                     DIARIVTHAEPRKE"
     gene            6060..8678
                     /gene="pepN"
                     /locus_tag="PLQ11_01185"
     CDS             6060..8678
                     /gene="pepN"
                     /locus_tag="PLQ11_01185"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HRK23545.1"
                     /translation="MRTDVAPVIRLADYQPPDFLVRTVEMLFDLRPEATRVRTVLSMQ
                     RNEHGFIGTPVVLEGDELTLVGLNLDGAPLPADRYTVAPDKLVIPNPPERPFTLTIET
                     LLDPAANTKLMGLYRSNGVYCTQCEAEGFRRITYFPDRPDVMSRYRVRIEADRDEAPI
                     LLSNGNPGAIGLIEGTNRHFAEWDDPFPKPSYLFALVAGDLGFISDTFTTASGKPVEL
                     NIFVEKGKEDRAGYAMDALKRSMRWDEQAFGCEYDLGVFNIVAVSDFNMGAMENKGLN
                     VFNDRYILASPETATDADYHNIEAIVAHEYFHNWTGNRITCRDWFQLCLKEGLTVFRD
                     QEFSSDMRSRAVERISAVRVLRSAQFVEDAGPLAHPVRPESYKEINNFYTATVYNKGA
                     EVIRMLKTLIGGSAFRRGMDLYFARHDGEAATVEQFIACFAEASGRDLSQFMRWYRQA
                     GTPVLTVRQSYDADARRLTLDFSQTIPATPGQPDKQPAVIPVLMGAIAADGREVIPNQ
                     VLVLESQRGTKTFENIAEPPVLSLLRGFSAPVRLDFDCDESALLTLARHDSDPFNRWE
                     ALQTYAMRLLKRSVAEVRAGRTPLTDAGLADAFRALLADGAIDPAFAALALTLPGDND
                     VAREIGADVDPDAIGAALDALSADIGAACLTAAEVRHEGLSAPRPFSADAESAGLRSL
                     RGAALAWLAWGDAKRGSATALAQFESATTMTDRLNALAVLNRLGGAGRDAAFQSFEKR
                     YRDEPLILDSWFTFQAMTRTRDALERVRKLMDHPGFVRTNPNRVRALIGAFANANPRQ
                     FHRPDGEGYRFLAGFIRDYDAINPQVAARLATAFRSYRTLEAGRQKKAAEALRSLQDS
                     GPLSRDVADIVERALA"
     gene            8945..11284
                     /locus_tag="PLQ11_01190"
     CDS             8945..11284
                     /locus_tag="PLQ11_01190"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRK23546.1"
                     /translation="MARIDATSARETFASSLGLAQSMGHPGYGRLTRAKPWIHRAVPI
                     LIVVFLAVVGTSGIVHLLQSRDAAIAGARKDLEVAAALAASRLKDALATRGSSEISEE
                     ITAVLVPPSALTDHRQLLIVNANGQVLGADPVGIGPVKTLGDLIGGADDLIRFGERNG
                     VVTVPLNRGGEAIAAARKLGEGPAGSLIAVVTQPMDAVLAGWFSLTQGVVTLMITLGG
                     MVASLGFAFYVEAARANRSDTFCSNVMMRIDTALGEARCGLWQWDIVHGRFYWSDSMF
                     ALIGYEPKQEPLAFGEVQALIERNDFDLMALANAMLRTGSHSTEQEFRMRHASGHWVW
                     LRARLQLNTDIDGSAPRLTGVIIDVTEQKRFAEQTRRADERLSDAINAISETFALWDS
                     SGRLVLCNAKFRALYGIDPCADIIGQHHDALIAASHVASSMDLTAAAHHNASRVYESQ
                     LADGRWVQISERQMDGGGFVSVGTDITALKEHERRLADSEHRLIDTVSDLRRSRQALQ
                     FKTIELADLAENYRAQRIAAEAANRAKTEFLANMSHELRTPLNAIIGFSEIMESRLFG
                     CLGSDKYEEYVGDIRKAGTGLMGIIDDILEMSRIEAGRVQLNREVADLSTIVQAAVMQ
                     VRPEAETKTIQLRTATPDLVSLSCDPRVLRHALVQFLRNSVKYTGSGGAIAVRVRRSG
                     DGVNIFIEDNGIGIAPENLSRFGAPFEVFSQQLDNGNKGSGLGAAIAKSLIELHGGRL
                     RVRSAIGIGTIILVHLPLSPVVDPGPLPDIGHPPSPLLH"
     gene            complement(11301..14225)
                     /locus_tag="PLQ11_01195"
     CDS             complement(11301..14225)
                     /locus_tag="PLQ11_01195"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF010706.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HRK23547.1"
                     /translation="MIETGVLAGRLVAGPKFSKAGLKQVDLLCAHDDEGAADLARVLG
                     QYPKARDLLAGIQEGSHYLWRLVVRDPGRAAALLADSPDHHVRRLVNIAIDTASLASD
                     ADAMRRLRRLKAEAALLIGMADLGGVWSVDQVVAGLSDFADACVIGAFDFVLRHAIAE
                     GKLAPPDPARPLQQLGMFILALGKHGAGELNYSSDIDLSVFFDPESATFRPSRDPQDQ
                     AVRMTKQMVRLLSDRNADGYVQRVDLRLRPDPGSTPIVVPVEAAYTYYETVGQNWERA
                     AMIKARPVAGDLAAGREFLRQLVPFIWRKYFDYAAIADIHAMKRQIYAVKGHESVAIA
                     GHDVKVGRGGIREIEFFVQTQQLVYGGRRPELRGSRTLDMLAALVTDGWITAAARDDL
                     AEAYRFLRSIEHRIQMVGDEQTQRLPTHDEELDRFARFCGYTRAGFEKALTRRLLTVE
                     RHYARLFESTPGLASEVGSLVFTGVEDDPATLETLRKLGFAKPAMVADTVRGWHFGRR
                     SAVTSARARETLTELIPALLESFGNSSDPDAALIAFDTALGKMPAAVELFSILKNHPG
                     VRDLFAEILGSAPRLAEIVARTPHVLDIVVDAGFTRLPEEVEIEARAEAQLQREKDAE
                     LFLEHARTMARHEQFLVAGQVLSRVLPAVDAGGAHTAIAEAFLRIVLRRTIADLAIRH
                     GRIPGASMAILGYGKLGSREMTAQSDLDLVIVYDCPGDAMSDGDKPLFPSEWFARLTQ
                     RLISALSAPLRNGALYEIDLRLRPSGRKGPVAVSLASFHSYQRDEAETWEHMALTRAR
                     PVAGDESLFGPLMAAIDDVIAKPRDPKKTAREIASMRALMDQERKASGRFDLKLMPGG
                     LVDIEFIAQFIVLTQAAPGLAAGTGVRAAIAAAGEAGRIARDDADLLLEAYALYSGLT
                     QALRIVLIDDKAVDEQPPAFKLKLAQATGLPSFKLLVTEITRLGKDVRRIFERTLGPV
                     RE"
     gene            complement(14222..15631)
                     /locus_tag="PLQ11_01200"
     CDS             complement(14222..15631)
                     /locus_tag="PLQ11_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRK23548.1"
                     /translation="MTGFAKLIRTTAFRLVVTYLLVFVLFAAALLGYIAWNTRRVLEA
                     QLNETIETEINSLAEQYRLGGPRRLAASIERRSREARGFFYLFADARGEQIAGNAVNI
                     GAVLQRQPGWHELSYTRAEDGASEQTFARVRLFRLPNNLTLVVGHDLDDRQRMRLVIR
                     QSMAMSLALVFVLGGIGAWVVSRRVLRPIDSMTEAASRIMAGNLAERLPVRGVGDELD
                     RLAEHLNLMLNRINELMAGLRDVSNNIAHDLRTPLTRLRAGAEEALRTASTPEELKAA
                     LDRTIEESDGLMRIFNALLMIARVEAGAVPGALADVELAGVVSDVAELYEPAAEEAGL
                     DLALSIEATPVVRGNRELLGQALANLIDNAVKYGRPADQGTKPPPLRLALSTHDGMAE
                     IAMSDHGPGIPPEAREKVLDRFVRLEKSRAQPGYGLGLSVVAAIARLHGGSIRIEDNS
                     PGTKAILSLPLARVEGVTR"
     gene            complement(15615..16295)
                     /locus_tag="PLQ11_01205"
     CDS             complement(15615..16295)
                     /locus_tag="PLQ11_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRK23549.1"
                     /translation="MKILVVEDDAEAASYLIKGLRESGHGVDHAVDGETGLEMALSAT
                     YDVAVIDRMLPRRDGLSLISSVRAEGRDLPILVLSALGQVDDRVMGLRAGGDDYLTKP
                     YAFTELLARVEALGRRRAASPAQTIYRVGDLELDRLAHSARRGGIAIDLQPREFRLLE
                     YLMRHAGQVVTRTMLLEHVWDYHFDPQTNVIDVHISRLRAKIDKNFDRPLLHTVRGAG
                     YMIRDGLR"
     gene            complement(16490..16972)
                     /locus_tag="PLQ11_01210"
     CDS             complement(16490..16972)
                     /locus_tag="PLQ11_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020090895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HRK23550.1"
                     /translation="MSRRTLFAALAVLGLAGPVAALSPSERLADPAQEARAREISAEL
                     RCLVCQNQSIDDSDADLAKDLRRIVRERIAAGDSNSAVKSFLVARYGEFVLLRPTFSL
                     HNALLWLLPVFALGAGVIVARRQFGNHRPVVDGAVRELTEDERAELARLTGEDTPPRT
                     "
     gene            complement(16969..18942)
                     /locus_tag="PLQ11_01215"
     CDS             complement(16969..18942)
                     /locus_tag="PLQ11_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016553312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HRK23551.1"
                     /translation="MIVELGHFALALALAVSLVAMVLPLWGALRNDGRLMAFGPPSAV
                     LQFLLVALAFGALTYAYVGSDFSVTNVFENSHTDQPMIYRFSGVWGNHEGSILLWVLI
                     LVLFGACVAAFGRSMPSPLRAATIAVQSSIVAAFLAFILFTSNPFLRVLPAPTEGRSL
                     NPVLQDPGLALHPPLLYIGYVGFSVVFSFAAAALILGRIDAAWARIVRPWILAAWTTL
                     TGGIALGSYWAYYELGWGGYWFWDPVENASLMPWLAGTALLHSVIVMERREALKVWTI
                     LLAVLAFSLSLLGTFLVRSGVLTSVHAFASDPARGVFILLILCAFIGGALLLFAMRGP
                     QLAGGALFSPVSREGSLVLNNLFLSAACAAVFIGTLYPLALEAVTGDKISVGPPFFNA
                     TFVPLSLPILLAMPFGQQLAWKRGELLPIAMRLFVAILAGAAVMLAVLALSSGREAIA
                     ALGLGLGAFVVVGSLDDLRIRAMRSGAGSFFSRLFGLPRTIVAAALAHMGVGLMVIGI
                     AATAFHEEQIANLEPGQTLNVGGIEISFEGMRRTTGPNFIATVGQFTARKDGVELMRM
                     EPGKRTYVGRSMPTSETSRLQRGLGEVYLAVGDAHEGKLDVRAYWKPYVLLIWLGPLV
                     MTLAGLLSLTDRRMRIAAPRRRVAPGAKPEPAE"
     gene            complement(18939..19403)
                     /gene="ccmE"
                     /locus_tag="PLQ11_01220"
     CDS             complement(18939..19403)
                     /gene="ccmE"
                     /locus_tag="PLQ11_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HRK23552.1"
                     /translation="MTRKQRRLALIGAAGLVLAVAVGLILTALNDRIVFFRSPAEVAQ
                     LNVQPGTRFRLGGLVENASVERPGAQQVVFRVTDGTRSMTVRYSGLLPDLFREGQGVV
                     CEGTLQADGSFNADTVLAKHDEKYMPREVSDALKKQGHWQGDAAPGGEGKPK"
     gene            complement(19400..20506)
                     /gene="ccmI"
                     /locus_tag="PLQ11_01225"
     CDS             complement(19400..20506)
                     /gene="ccmI"
                     /locus_tag="PLQ11_01225"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HRK23553.1"
                     /translation="MFFWLSAALLVVAALIPLIRSLVAKPATMDAASADVLFYKAQEA
                     EIERQLGAGLLSEGEASSLRTDAARKLLANARAAGGDGNAQTRTLGVVMALAVLVALP
                     VLASLFYLRQGKPGLPDMPFATRTDISRAEQDMARLLARLEAHLAENPDDLRGHELAF
                     PVYMRMARYDRAATSAGRIVALKGETGETLANFAEAMIFASQGEINEDARKALIASNK
                     LDPKLARSRFYLALLAEQDGNVASAVEQLRRLAEDVSDEAEKQAVNAQIARLSKATAL
                     PPGGDALKALPEAERNDAIRGMVDGLAQRLTEKGGSVEEWGRLIRSLAVLGQKDRAGE
                     MLGRARAGLADNTAALETLNALAGELGVKEGGAP"
     gene            complement(20603..21874)
                     /locus_tag="PLQ11_01230"
     CDS             complement(20603..21874)
                     /locus_tag="PLQ11_01230"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRK23554.1"
                     /translation="MTTAFDQRLRLYMKVLVADIAAVTDGTSTDVGNLGEPQFQIPLS
                     GWYWQIVKVDQEKSTDVILRVSRSLFSTRLPYLVSPLDPARQSDTGEGYLAGPDGRRL
                     RMLEQLIDLGDDNRYYISVGGNSADIDAQTRSFNTALILTFLLLGMALVASTIIQVRF
                     GLQPLARLRRGLEDIRRGEADSIGETFPDEVAPLAQELNQLVEANRDIVNRARMQVGN
                     LAHALKTPLSVLVNEASGDQTPLADKLREQTAVMKDQVQYYLDRARAATRAATIGSVT
                     DVDPVIAAFLRTFPKIYRDRNLSFAHSGVSGLRFRGERQDFEDMVGNLVDNAGKWASS
                     RVEITVAQVASDMAAAHPLRIIIDDDGPGLPPEGRIEATRRGRRLDETRPGSGLGLSI
                     VVDLAGLYHGRFELEESPLGGLRAVLTLPSV"
     gene            complement(21997..22671)
                     /locus_tag="PLQ11_01235"
     CDS             complement(21997..22671)
                     /locus_tag="PLQ11_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRK23555.1"
                     /translation="MRLLVVEDDKDLNRQLVTALQHAGYAVDTALDGEEGHFLGETEP
                     YDAVILDLGLPRMGGVEVLEKWRKAGRRMPVLILTARDRWSDKVQGFDAGADDYVTKP
                     FHLEEVLARLRALVRRAAGHATSEFQVGPVLLDTRSSKVVVDGNPVKLTSHEYRLLAY
                     LMHHQGRVVSRSELVEHLYDQDFDRDSNTIEVFIGRLRKKLGVDIIETVRGLGYLVGQ
                     PIKPPR"
     gene            complement(22700..23008)
                     /locus_tag="PLQ11_01240"
     CDS             complement(22700..23008)
                     /locus_tag="PLQ11_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018259819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23556.1"
                     /translation="MIRTCLFLLALMAALVPAAAASPAADGCLTDEQARTEILSHKLV
                     SIQQATRAARASVRGDVVSANLCRVNERLVYVLAILAPGGKVSRVTVDATTSAIRDQR
                     "
     gene            complement(23112..23891)
                     /locus_tag="PLQ11_01245"
     CDS             complement(23112..23891)
                     /locus_tag="PLQ11_01245"
                     /inference="COORDINATES: protein motif:HMM:NF017638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 108 family protein"
                     /protein_id="HRK23557.1"
                     /translation="MVARNFSRCLALTLRHEGGYSDHPADPGGATNLGITRAVLAEWR
                     GRPVTRAEVRALQRSEAEVIYRRLYWDAVGGDELPAGIDLAVFDYAVNSGVDRAVRTL
                     QRILGLAADGIAGVDTIRAARAARSVDVIRAICAARRGFLGRLKTFFVFGRGWLRRVE
                     AIERLALSLAADLSGAPLSSAHQSQPQESSAMNGSKSLIESRTVWANVIGLAAFGAGL
                     LGVDVGGVDQSAVVDSVLKVVTGASFIASMLFRIGATRKIV"
     gene            complement(23916..27875)
                     /locus_tag="PLQ11_01250"
     CDS             complement(23916..27875)
                     /locus_tag="PLQ11_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase/phage tail family protein"
                     /protein_id="HRK23558.1"
                     /translation="MATLVLQAAGSALGSALGGPILAAVGQAAGGLIGGMIDQRLMGS
                     GGRRYVEGPRLKQLDGITASEGSPIPRIYGRVRVGGQIIWATRFEEQVETTRSGKSGG
                     KSSGSRQPQTVTTTYAYFANVAIALCEGPIAFVRRIWADGKPLDLNGLTMRTYVGSQT
                     QMPDPLIVARSGAAPAYRGTAYVVFERLPLADYGNRLPQLSFEVVRPVDGLGGMIRGV
                     DMIPGATEFGYHTTVVSRNIGQGATQPENRNQLTAATDFEASVDALQALMPNATSVAL
                     VSSWFGTDLRAGECRIAPAVDRAAKTTSAGTLSGLDPFASAGPDQWSVADVTRGAARV
                     VTEVNGRAAYGGTPSDASIRAAIADLKARGLSVVFYPFVMMDIASGNALPDPWNAGAS
                     QPAFPWRGRITCHPAPGRAGSPDGTAAAAAQVATFFGTVSASHYSVTGGVISYSGPEE
                     WSYRRMVLHQAALCKAAGGVDAFIIGSELIGLTRVRSAAGVYPAVAELKLLAAEVRAL
                     LGPLVKIGYAADWTEYGSHVLSSGAEVRFPLDPLWSDANIDFVGIDYYPPVADWRDGT
                     SHLDAADWPDGQDVGYLKNRLGAGEAFDWYYASDADRIAQTRTPITDGAFNKPWTFRA
                     KDLVGWWSNAHVERVAGAETAATGWVASGKPIWLTEVGCPAVDKGANAPNLFPDAKSA
                     ESGLPPFSSGARDDAVQARYLEAVMRRFDPSLPGFEPAHNPLSTVYGGRMVDPARIHV
                     WAWDARPYPAFPHLQSVWGDGANWATGHWITGRIESLALDRLIRAVAADYALPLPCCR
                     GVDGMLDGYIIDRPMSARAALEPLLGLFAVEAGVRSGELCFFGRGRGEPRLIDRDDIV
                     PAKGGVEILRSRAQETELPRQITIGFADAEQQYKRSAVASRRLETASRREDASETTAV
                     LRRAEAQRLAEIALQDAWIQRETLEIGLRPGLIALETGDLVRIAGEPHTCQITRITDG
                     SERRCNLRIIEPAVFGLRSTDNALADWTPPRIPGPPLGLIVDLPADPATPSVLSWIGV
                     RAEPWVGPYTLWRSSDGASFSPVGTIGRAAILGETVSALPPGPLWRWDRAAALTVRLE
                     GGALAAMGDAGALAGSQVLAVQGADAAWELLSFSQAELIGPGTWRLARLLRGLAGSEA
                     QASRLAPPGSRAVLIDGALEPLYTAPEDIGSHAYFRISAAGRDHADPGALSFDHPAGN
                     AALRPLAPVFARARRLPAGIELTWIRRTRRDGDSWDVLEVPLSEGSEAYRVDIYSGAT
                     LKRRLATNMPQVLYPAADEIADFGSVQAALSVRIVQISQSIGDGTAHVATIPVRLSV"
     gene            complement(27875..28312)
                     /locus_tag="PLQ11_01255"
     CDS             complement(27875..28312)
                     /locus_tag="PLQ11_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HRK23559.1"
                     /translation="MTALTRPDIISAARAWIGTPYLHQASLRGIGCDCLGLLRGVWRE
                     VIGSEPETPPPYAPDWAEAGFDETFAQACRQHLVPVVVNAARPGDVLLFRWRSGLPAK
                     HAAILTAADHIIHAHDGAAVAEVPLGLWRRRIAFAFCFPGVID"
     gene            complement(28309..29187)
                     /locus_tag="PLQ11_01260"
     CDS             complement(28309..29187)
                     /locus_tag="PLQ11_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2163 domain-containing protein"
                     /protein_id="HRK23560.1"
                     /translation="MRAIPQPLASHLAGGATTLCRCWLLVRRDGTAFGFTDHDRDLTF
                     DGQIYSATSGLAPSEVESQLGLAVSTGEIGGALTDARLTEADIIGGRYDDAEITTWLV
                     NWADVSQRLMLDGSTIGEIRRMDQSFVAELRGPLHRYDQEQGRLYTQGCTADLGDAAC
                     GIDLAARAVTAAVTATDGRLNLTVAAIAATAASHFAGGRAELLTGANAGLTRAIQTHG
                     AGGTLALWEPLPQSLAVGDSLRLTPGCDKSFATCGLKFVNAANFRGFPHIPTPDFILT
                     YARPGEGGHDGGPLDP"
     gene            complement(29199..29837)
                     /locus_tag="PLQ11_01265"
     CDS             complement(29199..29837)
                     /locus_tag="PLQ11_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2460 domain-containing protein"
                     /protein_id="HRK23561.1"
                     /translation="MAFHEVRFPLDVSLRASGGPERRTDVVTMASGHEERNQRWAHSR
                     RRYDAGYGVKSFATLAAIIAFFEERRGRLHGFRFRDRLDWSSALPGAAILPGDQFLGN
                     GTGTRSTFQLVKRYGGQHAPYVRSIVKPVAGSVRIAVAGIEKVAGTDFTVDSTTGLVT
                     FQAGHLPGAGQAVTAGFAYDVPVRFDSDMLDIDIEAFEAGAIPKIPLVEILI"
     gene            29924..30160
                     /locus_tag="PLQ11_01270"
     CDS             29924..30160
                     /locus_tag="PLQ11_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019962736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HRK23562.1"
                     /translation="MSHTPHEIAAEFPDKAEKIHQLKMTNAHFARLVDEYHHVNRQIH
                     RIETEVEPTSDQVLETFKKQRLHLLDQVAGMLRA"
     gene            complement(30171..>30350)
                     /locus_tag="PLQ11_01275"
     CDS             complement(30171..>30350)
                     /locus_tag="PLQ11_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012253046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HRK23563.1"
                     /translation="ILPLARGADGRLGVAAQGGGGAAPVVVNIQTPDIASFARAESQI
                     SAQLARAVARGRRAT"
BASE COUNT         5845 a         9805 c         9696 g         5004 t
ORIGIN      
        1 gcttccggcg gggcgattga tggcagcaat gctgcctggc gggcagcgcc ggcccgggag
       61 cccgcatcgc cagcctgacg gcttgggctt gcctgtcccg gcatgccggc aactgaatgc
      121 cgggctgccg cagaggaggc attcgccgat tggatgctgg tcacaccagc ggcatcggcc
      181 tgagcagcgg ccgcttgcgc tgctgccctg gtggccgaac gctgcgccga cattttcgcc
      241 gcaacgcgcg gcggtgccag agcatcggcg aaactgtcga cttcggcgcg cgtgctcctg
      301 gccggaccgg aaccggcggc ggttccgccg acgcccatgg caaagccgga gcggcgcagc
      361 atggtctcgc cctcaggacc ggcaaccgag atccgtccga aatgattgac aacgcgtgtg
      421 ccactcgcac cgtcgtgccg gacagaggcg acaccgccgc ggacgccgag ggtggtcacg
      481 ggcgttcgga gggtggcgcc tccggtatgg ctggtctgcc cgccaacaaa gcgcagcgcg
      541 ccgcgcgtca gcgttgccgc catccggcca gaattgctga cgcgatcgaa cacgaattcg
      601 tcgatcacca cctggctgtt ggggccaatg gtcatcgtcg tgcgatcaac aaacagcact
      661 tgcagcgcgc cgttcgggcc ggtggtgagc gtctcgccat agagaaccga gctgccaacg
      721 ttgagcacgc gcgccgcgcc ggcgccgcgc gccttcgctg tcgggctgac ggcacctgcc
      781 gttcccactg tctcggcgac cgccgcagcc gaaaaccccg ccgccaccag aacaagcaat
      841 gaggcccgaa ccattccata catggcattc tcctcagaat cgcagcgtcg gcccgaacga
      901 gacactcaca ttccggcgag catagttggg caaggttgaa cggttgttga ccaattgcac
      961 ggtgatcccg aaaccaagac ggtcgctcac cggcgcatcc agcgagaccc cgagccgcca
     1021 ttcccggtcg cggcgacggc gaagcgggtc caccagcgga tcgggcgcat catagcgcgt
     1081 atgtgtccag ccgacaaagg gggccaacgt ccatttgcgc ccaagcgagg gcactgcaaa
     1141 accataggca acgccggcat cgaagccatg cgcccgatgc gcttgccggt cggcgcgggc
     1201 ctccgcgcgg cttgcctgaa gccgggcaaa ccagcgcagg ccaggcggga gcgtgccgga
     1261 aacggtgaca aagccgttgt gccagcgccc gttggactcg gcggctgtcg gcatgccgga
     1321 cgaggtgtgg aagatgcgcc ggtgcatctc gtagcccggc tcgaccaaaa tcgcgcccag
     1381 cgggatggca agggtcacac ccgcgccaac cgcatgggca aagggcgcat cgccgagtgt
     1441 cactccgctg gccagcagat aaggccgcac cgaccagccg ggcagcagat ccggttcaag
     1501 cgccagccgc gggcccagat ttgcctcgac aacgcccgca ttcagcgagg ccagccggaa
     1561 ctggcgcgca taatagccgg tcaattgcgc ttcgatcgta tcgccgcgct ggttctccag
     1621 atcatggaca aggcgcaggc tgccctgcac gaaggcgttc cagtccggct tgcgcagcga
     1681 cgtgttcggc agggaggtgt caaaaccgag cgcccgaacc agcggcgatg acggcgcggc
     1741 attggcattg ctctgccagc gcaagcccgt ctgcccgctg aagctccagc cagtgggttg
     1801 caggcgccgt tcgatctcga tcagaaacgc cgccgcccgt gcccgcgttg cctccggcgc
     1861 ctcaacagca gaggcccgct cgaaagccga ccgcgccaac tccaaagcac caaggcgcac
     1921 atacatctcg ccgatggcaa gatgggcggc ccaatcatcg ggatgggcaa gcacaacccg
     1981 ctccagcgcg ccgatgcctg cctcgaaatc gcccatgcgt gccgccagtt cagcatagcg
     2041 gcggctcagc gccgcatcgg ccggcgcctt caagacgcgc gcaaacagcg catcatggtc
     2101 ggccctgagc tgtggatcat cggaaatggg cgcaagctgc gctgcggcac tgccggccgc
     2161 cagccacatc gccaagccaa accacagcct gtggcgcaat gcaccgctcc tgaaccatca
     2221 aagtggcctt tttccccggg ctcctgccga cgaaaaggcg ttccttgcaa atcacttacg
     2281 taaaatatgc gcctttccga atcaatacaa tgcggcataa ccgtataggc attgccaaat
     2341 tcaaagtctt ttcgtcgatc acgggtcttt ccgctgtatc gtcagcgatg atgaacaaaa
     2401 atctacagct tgttcagtgc cctgccgccc ggatgttcat cagtgccgtg gcgttacacc
     2461 atgctccaga caaagcgcgc ggcggtcagc agcagaaagc agccaaagcc gatctccagg
     2521 cgccgcttgg gcgttgcatg ggcgagtcgt gcgccatagg gcgcaagaaa ggtcgaggtt
     2581 ggagcaacca gcaggaaccc gagcaccgat atgaaaccca gcgacagcgg cggcaggacc
     2641 gccatctggc tccagccggc cagcacatag cccaatgcgc cggggatcga gatcagcacg
     2701 ccaagtccgg cagaggttgc gaccgccgca tgcatcgaaa cgccgaacag cgtcaggaac
     2761 atgttggcga tcacaccgcc gccgatgccc atcagcgatg aggccaagcc gatgacgaag
     2821 ccgaagctgg ccagacccgc cctgcccggc aattgatcgc ccaggcgcca gtcatcgcga
     2881 ccggccagca gcttgatggc attgatcgtg gccaccacca cgaacacagc cttgagcacc
     2941 ttgccgggcg cataggctgc gatgacagcc cccagagcga cccctgcgat gaccggcaag
     3001 gcccagatct tcaacacgtc catcagggtc ttgcccttgg cgaggtgcgt gcggaacgac
     3061 tggatcgatg ttggaatgat gatggcgagc gaggtgccga cgcaaagatg catccgcgct
     3121 tcttccggca caccgacaat accgaaaatc tggtagagca ccggcacgat cacggcgccg
     3181 ccgccgacac cgaacagccc tgccagcaac ccggtgatca tcccggcgcc gagcaaagca
     3241 gcagcaaaaa gcagaagatc ggccagaggc agtccaagca tcgcagcggg ttccgactgt
     3301 catcccatgt ctggccgatt ggtgctggcc gggcggtgct tatccgtcta aaagaatttt
     3361 ctcccattgc caaatctcca gacaccatgc cgtcgaccgc cgcccatgcc ccgttcaacc
     3421 gcgtcacctg ggtggcgatc ggcgggctgg gcattaccca gatcatcggc tggggctcga
     3481 cctactacct tttgacgctg ctggggcggc ccatcgccac cgaactcggc ctttcccagg
     3541 cgttcgtctc ggccggcctg tcgctgatgc tgatcatggg cgcggtgttc ggaccggtcg
     3601 caggacgcgc gatggaccag cgcggagcgc ggccggtgat ggcgctgggc agcctgatag
     3661 cctccgccgg gctggtggcg ctcgccttcg tgcgtgggcc ggtcggctac atggctgcct
     3721 ggttcgtgat tggactgtcg gcagcgctga tcctctatcc agcggccttc accgcgctga
     3781 cacagatcgc cccgcatcag gcgcggcggg cgatttcctg gctgaccctg cctggcggac
     3841 tggcttcgac ggtgttctgg ccgctgaccg aggccctgct gacaatcatg gactggcgca
     3901 gcatctgcct gttctatgcg gcgctcaatc tctgtgtctg tctgccgctg catctggcgg
     3961 tgatcaggcg cgatccggcc gccgcagacc agcaggacga atcagggcca ttgccgcagg
     4021 gccttccggc cgacgcccgg cggatggcct ttctgctgtt tgcggcggcg ctggcgctca
     4081 attccctgct ggtcaccggc ctgctcaacc atttcatcgc catcatgcgc gatttcggac
     4141 aaccggcgca ggcggctgtt gcgattggca tggtgttcgg cctttcgcag acctcggcac
     4201 gcattctcga actgatcagc ggtgcccgca ccgatccgtt gatgctgggc gttgcagttg
     4261 ccgccggctt ccccctagct tttgcggcca tgatggtggc cccggcatcg ctggtggcgg
     4321 gcatggcctt tgcggtgatc cttggcatgt gcaacgggct gtggaccatc gtgcgtgggg
     4381 cgatggtgct gcggctgttc ggtgtcgccg gctatggcga gaacctcggc acgatcatcg
     4441 tcgcgcaggg actggcggcc ggggtggcgc cgatcctgct ggcatcgctg atggaaatcg
     4501 ccggggtgta tgcggccttc gttgcctgcg gccttgtgtc ggcaacggcg ctggttgcca
     4561 tggttctgct tttccgccat gcacgccgcg tcgaggcagc agcgagaccc ggaccatgac
     4621 tgatacggcg cgacagacca acgcagcacg caaggaatgg gcagcgcgca tttcggtgtt
     4681 tgccagcgcc tgcatcgcgc tgctcaagct ggcagcgggg ctggtttcgg gatcgctggc
     4741 actgctgtcc gaggccggcc acgccttgat cgaccttggc gcgacggttc tcacctggtt
     4801 tgcggtcaag accggtgaca aaccggctga tgacgagcac cattacggcc atggcaagat
     4861 cgaggctgtc gccgcgttgg cagagaccgg cctgcttatc gcgctcgcct gctatgtgct
     4921 ctacgaggcg attcatcgcc tgcggttcgg cggggaggtt ccggaaatca cctggccggt
     4981 gctggccgtg ctggtggttt cgatcttggt cgatttcaat cgctggatgc acctgcgcaa
     5041 ggtggcgaag gaaacccact cggtcgcact ggcggccgat gcgctgcact tctcatcgga
     5101 tctggtcagt tcgctgatgg ttctggtcgg cctcggcgcg gtgatggcag gctttccgaa
     5161 aggcgatgcg ctggcggcga tcgcggtggc gatcttcatc ctggtggcgg catttgaact
     5221 gggcaagcgc accgtcaata cgctgatcga tgcggcgccg catggcgcgc aggaccaggt
     5281 tgtcggaatt ctcagggaga tcaccggcgt catcgacgtg cggtcggtca aggtgcggcc
     5341 ggccggctcg acgttgttcg tcgaggcgct ggtcagcgtg ccgcggctgc tgccgcttga
     5401 ccgggtgcag cagatccgcg aggcagcggc agacgcgatc actgcggcca tgccggaagc
     5461 ggaagcgacg atcaccgtcg agccgaaggc gatggacgac gaaagcgtgc tcgaacgcgt
     5521 catcctgatt gccgcaaggc gacgcattcc ggtgcatcat gtcaccgtgc agacgatcgg
     5581 aaaaaggctc agtgtatcgc tggatgtgga ggtggacggc cggatgagtc tcgcagccgc
     5641 ccatcagatc gccacgcggc tggaggcggc gatccgcgcc gaattcggcc ccgacaccga
     5701 ggtcgagacg catatcgaac ccctggaggc ccagggcctg gtcggcattg atgttgacga
     5761 ggacgagagg atcgaactgg cccgcatgat cctggactgc gccaagcaga tcgacggcct
     5821 gacgcatgtg catgatctcc gggtgcggcg caccgacaag ggactggttg tcacgctgca
     5881 tctgcgcgct gatccgcagg ccagcgttgc cgatgtgcat gaatcggtgg accggctgga
     5941 acatctggcc cgtgcggcac gtccggacat cgcgcggatc gtcacgcatg ccgaacctcg
     6001 caaggagtga tcggctggtg tagtttggtg attcgcttca cgaactccgc gaggctgcca
     6061 tgcgcaccga tgttgctccc gttatccgcc ttgccgacta ccagccgcca gattttctcg
     6121 tcaggactgt cgagatgctg tttgacctcc ggcccgaggc aacgcgggtg cgcacggttc
     6181 tttcgatgca gcgcaacgag cacggtttca tcggaacacc ggtggtgctg gaaggcgacg
     6241 agctgacgct ggttgggttg aatctcgacg gagcaccgct gcccgccgac cgctatacgg
     6301 tcgcgcctga caagctggtg attcccaatc cgccggagcg cccgttcacg ctgacgatcg
     6361 agacgctgct cgatccggcc gccaacacca aactgatggg gctctatcgc tccaacggag
     6421 tctattgcac gcaatgcgag gcagagggtt tccgccgcat cacctatttc cctgaccggc
     6481 cggacgtgat gagccgctac cgcgtgcgca tcgaggccga tcgcgacgaa gcaccgattc
     6541 tgttgtccaa cggaaacccc ggcgcgatcg gattgatcga gggaacaaac cggcatttcg
     6601 ccgagtggga cgatccgttt cccaagccgt cctatctgtt cgcgctggtc gccggcgatc
     6661 tcggcttcat ctccgatacg ttcacgaccg cctcgggcaa gccggtcgaa ctcaacatct
     6721 ttgtcgagaa gggcaaggag gaccgcgccg gctatgccat ggatgcgctc aagcgctcga
     6781 tgcgctggga cgagcaggca ttcggctgcg agtatgacct tggcgtattc aacatcgtcg
     6841 ccgtgtcgga tttcaacatg ggggcgatgg agaacaaggg tctcaacgtc ttcaatgacc
     6901 gctacatcct ggccagcccg gagacggcga ccgacgccga ctatcacaac atcgaagcca
     6961 tcgtcgccca tgagtatttc cacaactgga ccggcaaccg catcacctgt cgcgattggt
     7021 tccaattgtg cctgaaggaa ggactgacgg tgttccgcga tcaggagttc tcctccgaca
     7081 tgcggtcacg ggcagtcgag cggatcagcg cggtgcgggt gctcaggtcg gcacagttcg
     7141 tcgaggatgc tggcccgctg gcacatccgg tacgcccaga aagctacaag gaaatcaata
     7201 acttctacac agccacggtg tataacaagg gcgccgaggt gatccgcatg ctcaagacgc
     7261 tgatcggtgg atcggcgttc cggcgcggca tggatctcta ttttgccagg catgacgggg
     7321 aggcggcaac cgtcgagcaa ttcatcgcct gttttgccga agcctcgggg cgcgatctca
     7381 gccagttcat gcgctggtat cggcaggccg gaacgccggt tctgacggtg cgccagagct
     7441 atgatgccga tgcccggcgt ctgacactgg atttttccca gaccatcccg gctacccccg
     7501 gccagcccga caaacagcca gccgtcattc ctgtactgat gggggcgatt gccgctgacg
     7561 gtcgggaagt gatccccaat caggtgctgg tgctggaaag ccagcgggga accaagacct
     7621 tcgagaatat cgccgagccc ccggttctgt cgctgctacg cggcttttcg gcgccggtgc
     7681 ggctcgattt cgattgcgat gagtctgccc tgctgacgct ggcgcggcat gacagcgatc
     7741 cgttcaatcg ctgggaagcg ttgcagacct atgcgatgcg cttgctcaag cgctcggtcg
     7801 ccgaggtgcg cgccgggcgc acgccgctga ccgatgcggg gctggccgac gcattccgtg
     7861 cactgctggc agacggcgcc atcgaccctg cgtttgccgc gctggcgctg accttgccgg
     7921 gcgacaatga tgttgcgcgc gagatcggcg ccgatgtcga tcctgatgcg atcggcgccg
     7981 cactcgatgc actttctgcc gacatcggcg cggcgtgcct gacagcggcc gaagtgcggc
     8041 atgaggggct gtccgccccg cgcccgttct cagccgatgc ggagagcgcc ggcctgcgct
     8101 cgctcagggg cgcagcgctc gcctggctgg cctggggcga tgccaaacgc ggcagcgcga
     8161 ccgcgcttgc ccagtttgaa tccgccacga cgatgaccga taggctgaac gcgctggctg
     8221 tgctcaatcg tctgggcgga gcgggccgtg atgcggcctt ccaatctttc gagaagcgct
     8281 atcgcgatga accgctgatc ctggactcgt ggtttacctt ccaggcgatg acccggacgc
     8341 gcgatgcgct ggagcgggtg cgcaaactga tggaccatcc cggcttcgtg cgcaccaatc
     8401 ccaaccgcgt gcgcgcgctg atcggcgcct tcgccaatgc caatccacgt caattccatc
     8461 gtcccgacgg tgaaggctat cgcttcctgg cgggtttcat ccgcgactat gacgcgatca
     8521 atccgcaggt cgctgcccgg ctggcgacag cctttcgcag ctaccgaacc cttgaggccg
     8581 gcaggcagaa gaaggccgca gaggccctcc gctcgctgca ggatagcggt cctttgtcgc
     8641 gcgatgtcgc cgatatcgtc gaacgggcct tggcataacg tgcaagccat accgtatgga
     8701 tacaagaaat aaaatcaaac ataacaagaa taaaaagcga acatcaagat ctgaatggca
     8761 gaagacctac caacctgaga cgtctgctga ttctcgaccg gccggcacag ggcaaaaaac
     8821 taaaaaaacg gtaatttttc ttctggacag cgcccgcaag ccagattcaa ctgtgactga
     8881 ttcggggcgc gtcgctggtc ctcgtcgggc aaactttcgt tcggttttat cgttggggga
     8941 cagcatggcg cggattgacg cgaccagtgc gcgggagacc tttgcgtcaa gtctgggatt
     9001 ggcacaatcg atgggccatc ccggttatgg gcgcctgacg cgggccaaac cctggatcca
     9061 ccgggcggtc ccaatcctga tcgtggtctt tcttgcggtg gtgggcacca gcggcatcgt
     9121 gcatctgctg caatcgcgcg atgcggccat cgccggcgcc cgcaaggatc tggaagtggc
     9181 ggccgcgctg gctgcgtcgc ggctgaaaga cgccctggcc acccgtggct ccagcgagat
     9241 cagcgaggag atcacggcgg ttctcgtgcc gccatcggcg ctgaccgacc atcgccagct
     9301 gctgatcgtc aatgccaatg gacaggtgct cggcgccgat ccggtgggta tcggcccggt
     9361 caagaccctc ggcgatctga tcggcggggc tgacgacctc atcaggttcg gcgaacgcaa
     9421 cggcgttgtg acggttccgc tcaaccgcgg cggcgaagcc atcgcggcgg cgcgaaaact
     9481 gggggagggc ccggccggtt ccctgatcgc agtggtcacc cagccgatgg atgcggtgct
     9541 ggccggctgg ttctcgctga cccagggggt ggtcacgctg atgatcacac tgggcggcat
     9601 ggtcgcctcg ctgggctttg ccttctatgt cgaggctgcg cgggccaatc ggtcggatac
     9661 gttctgctcg aatgtcatga tgcggatcga caccgcgctg ggcgaagcgc gctgcggctt
     9721 gtggcaatgg gatatcgtgc acggcagatt ctactggtcc gactccatgt ttgcgctcat
     9781 cggctacgag cccaagcagg agccgcttgc cttcggcgag gtgcaggcgc tgatcgagcg
     9841 aaacgacttt gacctgatgg cgctggccaa tgcgatgctc aggacagggt cgcattccac
     9901 agagcaggag ttccggatgc gccatgccag cggccattgg gtctggctgc gcgcccggct
     9961 tcagctcaat accgatatcg acggctcggc cccacggctg accggtgtca tcatcgacgt
    10021 gaccgagcag aagcgttttg ccgagcagac acggcgcgct gatgaaaggc tgtccgatgc
    10081 gatcaacgcg atttccgaaa ccttcgcgct gtgggattcc tcaggccggc tggtgctgtg
    10141 caatgccaag ttccgcgcgc tttatgggat cgacccctgt gccgacatca tcggccagca
    10201 ccatgacgcc ctgattgccg cctcgcatgt tgccagttcg atggacctga cggcggcggc
    10261 gcaccacaat gcgtcccgcg tttacgaaag ccagcttgcc gatggccgct gggtgcagat
    10321 ttccgagcga cagatggacg gcggcgggtt cgtctccgtg ggcaccgaca tcacggcgct
    10381 caaggaacac gagcggcggc tggccgacag cgagcatcgg ttgatcgaca ccgtgtccga
    10441 tttgcgtcgg tcgcgccagg cgctgcagtt caagaccatt gaactggccg atcttgccga
    10501 gaattaccgc gcccagcgga tcgcggccga agccgccaac cgcgccaaga ccgagttcct
    10561 ggccaacatg agccatgaat tgcgcacgcc gctcaatgca atcatcggct tttcggaaat
    10621 catggaaagc cggctgttcg gctgtctcgg ctcggacaaa tatgaggaat atgtcgggga
    10681 catccgcaag gccggcaccg gactgatggg catcatcgac gatattctcg agatgtcgcg
    10741 catcgaggcc gggcgggtgc aactcaaccg cgaggtcgcc gacctttcaa cgatcgtgca
    10801 ggctgcggtc atgcaggttc gcccggaggc cgagaccaag acaattcagc tgcgcacggc
    10861 aacgccggat ctggtgtcgc tctcctgtga tccgcgcgtg ctgagacatg cgctggtgca
    10921 attcctgcgc aactcggtga aatacaccgg ctccggtggg gcgattgcag tgcgggtgcg
    10981 ccggagcggg gacggcgtca acatcttcat cgaggacaac gggatcggta tcgcgccgga
    11041 aaacctctcc aggttcggcg cgccgttcga ggtgttcagc cagcagctcg acaacggcaa
    11101 caagggttca ggactgggcg ctgccatcgc caaatcgctg atcgaactgc atggcggccg
    11161 gctgagagtg cgctctgcga tcggaattgg caccatcatt ctggtccatc tgccgctgtc
    11221 gccagtggtt gatcccggcc ccctgcccga catcggtcac ccgccgagcc cgctactgca
    11281 ttaaccctcc gccggctgct ctactcccgc accggcccga gcgtccgctc aaagatacgg
    11341 cgcacgtcct tgcccaggcg ggtaatctca gtgaccaaaa gcttgaaact gggcaggccg
    11401 gtggcctgtg ccagcttcaa cttgaaggct ggtggctgct catcaaccgc cttgtcgtcg
    11461 atcagcacga tcctgagcgc ctgggtcagg ccggaataga gcgcgtaggc ctccagcagg
    11521 agatcggcat catcgcgggc gatgcggccg gcttcgccgg cagcggcaat cgccgcccga
    11581 acgccggtgc cggctgcaag gcccggcgcc gcctgcgtga gaacgatgaa ctgggcaatg
    11641 aattcgatat cgaccagccc gcccggcatg agtttgagat caaagcggcc gctcgccttg
    11701 cgttcctgat ccatcagtgc ccgcatggag gcgatttcgc gggcggtctt cttggggtcg
    11761 cgcggctttg cgatgacatc gtcgatcgca gccatcagcg gaccgaacag gctttcgtcg
    11821 ccggcgacgg ggcgggcccg cgtcagggcc atatgctccc aggtttcggc ttcatcgcgc
    11881 tggtaggaat ggaacgaggc gagcgacacc gccaccggcc ccttgcgacc ggatggccga
    11941 agccgcaggt cgatctcgta gagcgcgccg ttgcgcaaag gcgccgacag cgccgaaatc
    12001 agccgctggg tcagccgggc gaaccattcg gaggggaaca ggggcttgtc accatccgac
    12061 atcgcatcgc ccgggcaatc gtagacgatc accagatcaa ggtcggactg ggcggtcatt
    12121 tcgcgtgagc cgagcttgcc ataacccagg atcgccatgc tcgcaccggg gatgcgtcca
    12181 tgcctgatcg ccagatcggc gatcgtgcgg cgcagcacga tcctgaggaa cgcctcggcg
    12241 atcgccgtat gcgccccgcc agcatcgacc gccggcagaa cgcgcgaaag cacctgaccg
    12301 gcgacgagaa actgctcgtg acgggccatg gtgcgggcat gttccagaaa cagttcggcg
    12361 tccttctcgc gctgcaactg cgcctcggcg cgggcctcga tctctacctc ctccggaagg
    12421 cgggtgaagc cggcatcgac cacaatgtcg agcacatgcg gcgttcgggc gacgatctcg
    12481 gcaagacgcg gcgccgagcc gagaatttcg gcaaagagat cgcgcacgcc agggtggttc
    12541 ttcaggatcg agaagagttc gactgccgca ggcatcttgc ccagcgcagt atcgaaggca
    12601 atcagcgccg catcgggatc ggaggaattg ccgaaggatt ccagcagcgc cgggatcaac
    12661 tctgtcaatg tctcgcgggc gcgcgcactg gtgaccgccg agcgccggcc aaaatgccag
    12721 ccgcgcaccg tatcggccac catcgccggc ttggcgaagc cgagcttgcg cagcgtttcc
    12781 agcgtcgccg ggtcatcctc gacgccggtg aacaccagac tgccgacttc cgaggcaaga
    12841 ccgggggtcg attcgaacag ccgggcatag tgccgctcaa cggtgagcag ccggcgggtc
    12901 aacgcctttt cgaaccccgc ccgggtatag ccgcagaagc gggcgaaccg gtcaagttcc
    12961 tcatcatgcg taggcagccg ctgagtctgt tcatcgccga ccatctgaat gcgatgctcg
    13021 atggagcgca ggaagcgata ggcttcggca aggtcgtcgc gtgccgccgc agtgatccag
    13081 ccatccgtga ccagcgccgc cagcatgtcg agcgtgcgcg agcccctgag ttccggtcgg
    13141 cggccgccat agacgagttg ctgggtttga acaaaaaact cgatctcgcg gataccaccg
    13201 cgcccgacct tcacgtcatg gccggcgatg gcaacacttt catgcccttt gacggcgtaa
    13261 atctgtcgct tcatcgcgtg aatgtcagca atcgcggcgt aatcgaaata cttgcgccag
    13321 atgaacggca ccagctggcg cagaaattcc cgccccgccg ctagatcgcc ggcaaccggc
    13381 cgcgccttga tcatcgcagc gcgttcccag ttctggccaa cggtctcgta gtaagtatag
    13441 gcagcttcaa cgggaacgac gatcggcgtc gagccggggt ccggtcgcaa acgcaggtcc
    13501 acgcgctgga catagccatc ggcattgcga tcagaaagca gccggaccat ctgcttggtc
    13561 atccggacag cctgatcctg cggatcgcgg gagggacgga acgtcgccga ttccggatcg
    13621 aaaaagacgc tcaggtcaat gtcagaggag taattgagct cccctgcccc gtgtttgccc
    13681 agggcgagga tgaacatgcc gagctgctgc agcggccttg ccggatcggg cggggcgagc
    13741 ttgccctcgg cgattgcatg gcgcagcacg aaatcaaacg cgccgatgac gcaggcatcg
    13801 gcaaaatcgc tgagcccggc aacaacctga tcaacactcc acacgccgcc aagatcggcc
    13861 atcccgatca gcaaggcggc ctcagccttg agacggcgca gacgccgcat cgcatcggca
    13921 tccgaggcga ggctggcggt gtcgatcgcg atattgacca ggcgtcggac atgatgatcc
    13981 gggctgtcgg ccagcagcgc cgccgcccgc ccggggtcac ggaccacgag acgccagaga
    14041 taatgcgagc cttcctgaat gccggccagc agatcgcgcg ccttcggata ctggccaagc
    14101 acacgggcca gatcggcagc gccttcgtca tcatgggcac agagcagatc gacctgcttc
    14161 aatcccgcct tggaaaactt tggcccggcg acgaggcgcc cagccagaac tccagtctca
    14221 atcaccgcgt cacgccctcc actctggcga gcggcaagga tagtatggct ttggtgccgg
    14281 ggctattgtc ttcgatgcga atggaaccgc catgcaggcg cgcaatcgcc gcaaccacgc
    14341 tgagaccaag gccgtaacct ggctgggccc ggctcttctc aagccttaca aagcgatcca
    14401 gcaccttttc ccgcgcctcg ggcggaattc ccggaccgtg atcggacatg gcgatttcgg
    14461 ccatgccgtc atgtgtggaa agcgcaagac gcaagggtgg cggttttgtg ccctgatccg
    14521 ccgggcgacc atatttgacc gcattgtcga tgagattggc gagagcctgt ccgagaagct
    14581 cgcggttgcc gcggacgacc ggtgtcgcct cgatcgagag ggcaaggtcc aatccggcct
    14641 cctcagctgc cggctcgtag agctcggcca cgtcggagac gacccccgca agttcgacat
    14701 cggccaacgc cccaggaaca gcaccggcct cgacgcgggc gatcatcagc aaggcattga
    14761 atatgcgcat caacccgtcg gattcctcga ttgtcctatc aagcgctgcc ttgagttcct
    14821 caggtgtgga ggcggtgcgc aatgcctctt ccgcgccggc acgaagccgc gtcagcggtg
    14881 tgcgcaggtc atgggcgatg ttgttggaga catcgcgcag gcccgccatc agttcgttga
    14941 ttcggttcag catcaggttg agatgctcgg ccaggcggtc gagctcgtcg ccgaccccgc
    15001 gcaccggcag gcgctcggcc agatttcccg ccatgatgcg gctggcggcc tctgtcatgc
    15061 tgtcgatcgg acgcagcacc cggcgtgaca cgacccaggc gccgatgccg cccagcacga
    15121 aaaccagcgc cagcgacatg gccatcgact ggcggatcac caaacgcatg cgctgacgat
    15181 catcgagatc gtggccgacg accagggtca gattgtttgg cagacgaaac agacgcactc
    15241 gggcgaaagt ctgctcggaa gcgccgtcct cggcgcgggt ataggaaagt tcgtgccagc
    15301 ccggctggcg ttgcaggacc gcgccgatgt tgacggcatt gccggcaatc tgttcgccac
    15361 gcgcatcagc gaacagatag aagaagcccc gcgcctcgcg gctgcgccgt tcgatcgagg
    15421 cggccagccg tcgcgggcca ccgaggcgat actgttcggc cagactgtta atctcggtct
    15481 cgatggtctc gttgagctgc gcttccagca cccggcgcgt attccaggcg atgtaaccca
    15541 gcagagccgc tgcaaacagc acgaatacca gaagataggt caccacaagc cggaaggccg
    15601 tggtgcgtat gagcttagcg aagcccgtca cggatcatgt atccggcgcc acgcaccgta
    15661 tgcagcagcg ggcggtcgaa gttcttgtcg atcttggcac gcaggcggga aatatggacg
    15721 tcgatgacat tggtctgcgg atcgaaatga tagtcccaga catgttcgag cagcatcgtg
    15781 cgcgtcacga cctgtccggc atggcgcatc aggtattcca agagccggaa ctcgcgcggc
    15841 tgaagatcga tggcgatgcc gccgcgccgg gcagaatggg ccagccgatc cagttccaga
    15901 tcgccaaccc ggtagatcgt ctgcgccggc gaggccgccc gccggcggcc cagcgcttcg
    15961 acgcgcgcca gaagttcagt aaaggcatag ggcttggtga gataatcatc gccgccagcg
    16021 cgcaggccca tgacacgatc atcaacctgc ccaagcgccg aaagcaccag gatcggcaag
    16081 tcacgccctt cagccctcac ggagctgatc agcgaaaggc cgtcacgacg gggcagcatg
    16141 cggtctatga ccgcaacatc ataagtagcg ctcagcgcca tttcgaggcc agtctcgcca
    16201 tcgaccgcat gatcgacacc atggccggat tcgcgcaagc ccttgatcag ataacttgca
    16261 gcttcggcat catcctcgac gaccagaatc ttcatgggag gtctttagca cattcagaca
    16321 tgaaccggca ggctgaacct gttgaatgat ccagcctgcc ggcaaggtga tcccggaaca
    16381 gcttcgccat ttggggggcg acggggggtg aacagcgggc ctctccggaa gcgctctcac
    16441 cttgtcagtc cggccgtttc cgcaacctgt ccggagaatg acattttcgt catgtccggg
    16501 gcggagtgtc ctcgccggtc aagcgggcaa gctcggcgcg ctcatcttcg gtcaattccc
    16561 gaaccgcacc gtccaccacg ggccggtgat taccgaactg gcggcgagcc acgatgaccc
    16621 cggcccccag ggcaaaaacc gggagcagcc acaacaatgc attgtgcagg gaaaaggtcg
    16681 gccgcaacag aacgaattct ccatagcggg caaccaggaa tgatttgacc gcactgttgc
    16741 tatcgccagc ggcaatacgt tcgcgcacta ttctccgcag gtccttggcc agatcggcgt
    16801 cagaatcgtc aattgactga ttctggcaga cgaggcagcg caattcggcg gagatttccc
    16861 gggcacgggc ttcctgcgcc gggtcagcca gacgttcgga cggcgacagc gccgctaccg
    16921 gcccggcaag gcccaataca gcaagcgcgg cgaaaagcgt gcggcggctc attcggcggg
    16981 ctccggcttg gcacccggcg ccacgcgccg tcgcggagca gcaatgcgca tccgccgatc
    17041 ggtgagtgat agaaggccgg caagagtcat caccaatggc cccagccaga tcagcagcac
    17101 gtagggcttc caataggccc gcacatcgag cttgccttca tgcgcatcgc cgactgcaag
    17161 gtagacctcg ccgagtccgc gttgcagacg tgatgtctct gacgtcggca tggagcggcc
    17221 gacataggtg cgcttgcccg gttccatgcg catcagttcc acaccgtcct tgcgggcggt
    17281 gaactggcct acggtggcaa tgaaattcgg gccggtggtc cgccgcattc cttcaaagct
    17341 gatctctatc ccgcccacat tcagcgtctg gccaggttca aggtttgcaa tctgctcctc
    17401 atggaaagcg gtggcggcaa tgccgatcac catcaggccg acacccatat gcgccagcgc
    17461 cgctgcaaca atggtgcgcg gcagaccgaa cagacgggag aagaacgacc cggcgccgct
    17521 gcgcatcgcc cgaatgcgca ggtcatccag ggatcccacc acgacaaaag cgcccagccc
    17581 caaccccaga gcggcaatcg cctctcgccc gctggacagt gccagcaccg ccagcatcac
    17641 cgcagcgccg gccagaatcg ccacgaacag gcgcatggcg atcggcagca gttctccgcg
    17701 cttccacgca agctgctgac caaacggcat agccagcagg atgggcagcg acagcggcac
    17761 gaacgtagcg ttgaagaacg gcgggcccac cgagatcttg tcgcccgtga ccgcttccaa
    17821 tgccagagga tagagcgtgc cgataaacac ggccgcacag gcagcggaga gaaacaggtt
    17881 gttgagcacc aggctgccct cgcgcgagac cggcgagaac agcgctcccc cggccagctg
    17941 cggtccacgc atcgcaaaga gcagcaacgc tccgccgatg aaggcgcaga gaatgagcag
    18001 gatgaaaacg ccgcgcgccg ggtcggaggc aaaggcatgc accgaggtca gcacgcccga
    18061 acggacgagg aaggtgccga ggagcgacag cgaaaaggcc agcaccgcaa gcaggatggt
    18121 ccagaccttc agcgcttcgc gccgctccat aacgatcacg gaatgcagga gcgcggtgcc
    18181 agccagccag ggcatcagcg aggcattctc gaccggatcc cagaaccagt agccgcccca
    18241 gcccaattcg taataggccc agtatgaccc aagcgctatg ccaccggtca gggtcgtcca
    18301 ggcggccaga atccacggcc taacgatgcg ggcccaggcg gcatcgatgc ggccgagaat
    18361 cagcgccgca gccgcgaagg aaaacaccac cgaaaagccg acatagccga tgtagagcag
    18421 cggcggatgc agcgcgaggc cgggatcctg caagacagga ttgagcgacc gcccctcggt
    18481 cggagccggc aggacgcgca ggaacggatt ggaggtgaac aggatgaagg caaggaaagc
    18541 ggcaacgatc gaggattgta cagcaatcgt cgccgcacgc agtggcgacg gcatcgagcg
    18601 gccgaaagcc gcaacgcagg cgccgaacag caccagaatc agcacccaca gaaggatcga
    18661 gccctcgtgg ttgccccaga cgccggagaa ccggtagatc ataggctggt cggtatggct
    18721 gttttcgaaa acattggtga ccgaaaagtc cgatccgaca taggcatagg tgagcgcgcc
    18781 aaaggccagg gcgaccagca gaaactgcaa cacggcagag ggcgggccga atgccatcag
    18841 ccggccgtca ttgcgcaagg cgccccaaag cggcaacacc atcgccacaa gcgacaccgc
    18901 aagcgccaga gcgagggcga aatggccgag ttcgacgatc atttcggttt gccctcccca
    18961 ccgggcgcgg catcaccctg ccaatgcccc tgcttcttca gcgcatccga tacttcgcgc
    19021 ggcatgtatt tctcgtcgtg cttggcaagc acggtatccg cgttgaaact gccatcagcc
    19081 tgcagcgtgc cctcacagac aacgccctgt ccctcccgga acagatcagg cagcaggccg
    19141 gaataacgca cggtcatcga ccgcgttccg tcagtcaccc tgaagacaac ctgctgggca
    19201 ccgggacgct cgacactggc attttcgacc agaccgccca gacgaaaccg cgtgccaggc
    19261 tggacattga gctgggccac ctccgccggc gagcggaaga acacgatgcg atcattgaga
    19321 gcggtcagga tcagaccgac cgcgaccgcc agaaccaatc ctgccgcacc aatcaaggca
    19381 agccggcgct gcttgcgcgt catggcgcgc ctccctcttt gacacccagt tctccggcca
    19441 gagcattgag tgtctccagc gcggcggtat tgtccgcaag gccggcgcgc gcgcgcccga
    19501 gcatctcgcc agcacgatcc ttttgcccga gcacagccag cgaccggatc agacgccccc
    19561 attcctccac gctgccaccc ttctcggtca ggcgttgagc cagcccgtcg accatgccac
    19621 ggatggcatc gttgcgctca gcctccggca gggctttcag cgcatcgccg ccaggcggca
    19681 gggctgttgc cttggacagg cgggcgatct gggcattgac cgcctgcttc tctgcctcgt
    19741 cgctgacgtc ctcggcgagg cgtctcaact gttcgacggc cgaggcgaca ttaccgtcct
    19801 gctcggcaag cagggcaaga tagaagcggc tgcgcgccag cttggggtcc agcttgttgg
    19861 atgcgatcag cgccttgcgc gcgtcttcgt tgatctcgcc ttgcgaagca aaaatcatgg
    19921 cctcggcaaa attggcaagc gtttcgcccg tttccccttt caaagccaca atgcggcccg
    19981 cagaggtcgc cgcgcgatca taacgggcca tccgcatgta aaccggaaaa gccagttcat
    20041 gaccgcgcag atcgtcagga ttctctgcca gatgggcctc gagccgcgcg agcagccgcg
    20101 ccatgtcctg ctccgcacgg gaaatgtcgg tgcgcgtcgc aaaaggcatg tcaggcagtc
    20161 ccggcttgcc ttgccgaaga tagaacaggg aggccagaac cggcaatgcc accagcacgg
    20221 ccagcgccat gaccacacca agggtccggg tctgggcatt gccatcgccg ccggcagccc
    20281 tggcattggc cagaagcttg cgggcggcat ccgtgcgcaa gctgctggcc tcgccctcgc
    20341 tcagcaggcc ggcgccgagc tgacgttcga tttcagcctc ctgcgccttg taaaacagaa
    20401 catcggcgct ggcagcatcc atcgtcgccg gtttggcaac cagcgaacgg atcagcggga
    20461 tcagggcggc gacaaccagc agcgccgcag aaagccagaa aaacatggca gggggcatac
    20521 cccagcttcg agccaaaagg aacgcgattt atcaagtcgc gtgcgcgtcc ttccggcaga
    20581 aacccgacca aattgcggca ggtcaaacgc tcggcagggt cagcacggcc cgcaacccgc
    20641 caagtggcga ttcttcaagt tcgaagcggc cgtgatagag gccggcaaga tcaacgacga
    20701 tcgacagccc cagcccggag cctggtctgg tctcatcgag ccggcgcccc cgccgcgtgg
    20761 cctcgatccg gccttcaggc ggaaggcccg gtccgtcatc gtcgatgatg atgcgcaagg
    20821 gatgggcagc agccatgtcc gacgccactt gcgccactgt gatctccacc cgtgacgatg
    20881 cccactttcc ggcattgtcg acaagattgc cgaccatatc ctcgaaatcc tggcgttcgc
    20941 cgcgaaatcg caggcctgac acgcccgaat gggcgaagga caggttccgg tcgcggtaga
    21001 ttttcggaaa agtccgcaaa aaggcggcaa tgacggggtc gacgtccgtc acggagccta
    21061 tggttgcggc gcgcgtcgcc gcccgggcac ggtccagata gtattgcacc tgatccttca
    21121 tcaccgccgt ctgctcgcgc agcttgtcgg caagcggagt ttgatcgccg gacgcctcgt
    21181 tgaccagcac ggagagcggc gtcttgagcg catgggcgag attgccgacc tgcatccgcg
    21241 cgcgattgac aatatcgcgg ttggcctcga cgagctggtt gagttcctgg gcaagcgggg
    21301 ccacctcgtc gggaaaggtc tcgccgatgc tgtcggcctc accacggcga atgtcctcga
    21361 gaccgcggcg caggcgcgcc agcggctgga gaccgaaacg cacctgaatg atcgtggagg
    21421 cgacaagcgc catgcccagc agcaggaacg tcaggatcaa tgccgtgttg aaactgcggg
    21481 tctgcgcatc aatgtcggca gaattgccgc cgaccgaaat gtagtaacgg ttgtcgtcgc
    21541 caagatcgat caactgttcc agcatgcgaa ggcgccgacc atccggaccg gcaagatagc
    21601 cttcgccggt gtcagactgg cgggcaggat ccagcgggct gaccagatag ggcaggcggg
    21661 tggaaaagag cgaccgcgac acccgcagga taacatccgt gcttttttcc tgatcgacct
    21721 tgacgatctg ccaataccag cccgacagcg gaatctggaa ctgcggctcg ccgagattgc
    21781 cgacatctgt gctggtgcca tcggtcaccg ccgcgatgtc tgccaccaga accttcatgt
    21841 aaagccgcag ccgctgatcg aatgcagtgg tcacggcgcg gctgttcacc gatgacagaa
    21901 ccacgccagc agagagtaga atgaggctgc acagcgccgt tgccgacagc agcaggcggc
    21961 cggataggga cgtcagcctg ccgcccgcca ttggcgtcag cgcggtggct tgatgggctg
    22021 gccgaccaaa tagccgaggc cgcggacggt ttcgatgatg tccacgccga gcttcttgcg
    22081 cagacgcccg atgaacactt cgatggtgtt ggaatcgcgg tcaaaatcct ggtcgtagag
    22141 atgttcgacc agttcagagc gtgacaccac ccgcccctgg tgatgcatca gataggcaag
    22201 cagtcgatac tcgtggctgg tgagtttgac cggattgcca tcgaccacca ccttgctgga
    22261 acgggtatcg agaagcaccg gaccaacctg aaattcactg gtcgcatggc ccgcggcccg
    22321 gcgcaccaga gcacgaagac gcgcaagcac ttcctcaaga tgaaacggct tggtcacata
    22381 atcgtccgcg ccagcatcaa acccctgaac cttgtcgctc cagcggtcgc gcgccgtgag
    22441 gatcagaacc ggcatccgcc gcccggcctt gcgccatttc tcaagcacct cgacaccgcc
    22501 catgcgcggc aggccgagat cgaggatcac cgcatcatag ggttcggtct cgccgaggaa
    22561 atgaccttcc tccccatcga gtgccgtgtc gaccgcgtag cccgcatgct gaagggccgt
    22621 gacaagctgg cgattcagat ccttgtcatc ctcgacgacg agcagtctca tgatggaaag
    22681 cttccgggtg ttgaatgggt cagcgttggt cccggatggc gctggtcgtc gcatcgacag
    22741 tgacgcgcga gaccttgccg ccgggggcca ggatggccag aacatacacc agtcgctcgt
    22801 tcacgcgaca gagatttgct gagaccacgt ccccgcggac cgaggcacgg gccgcacggg
    22861 ttgcctgctg gatgctgacg agcttgtgcg aaaggatttc cgtgcgggcc tgttcgtccg
    22921 tcaggcagcc atccgccgca ggcgaagcag cggctgcagg gaccagcgcg gccatcaggg
    22981 ccagcaggaa cagacaggtt cggatcatcc ggcaatcgtg gttcccagcc cctgaacccg
    23041 tcatgaacgc gctggcgcga gatcacgctg tgccttgacg gttcaatcag gacacagcgt
    23101 gatctggggc gtcagacgat cttgcgggtg gcgccgattc tgaacagcat cgatgcgatg
    23161 aagctcgccc cggtgacaac cttgaggacg gaatcgacaa cagcgctctg gtcaacgccg
    23221 ccgacatcga cccccagaag accggccccg aaagcggcca atccgataac attggcccag
    23281 accgtgcggc tttcgatcag tgatttgctt ccattcatgg cacttgactc ctgtggttgg
    23341 gattggtggg cggatgacaa gggtgcgccg gataggtctg cggcaagcga gagcgcgagg
    23401 cgctcgatcg cttcgacacg ccgcagccag ccgcgcccga acacgaagaa cgtcttcagc
    23461 cgaccgagaa agccgcgccg tgccgcacag atggctcgga tcacgtccac agatctggca
    23521 gcccttgcgg cacggattgt gtcaacgcca gcgatgccat cggccgctaa gcccaggatc
    23581 cgctgcaagg tgcgcacggc gcgatcgacg ccggaattga ccgcgtagtc aaacaccgcg
    23641 agatcgatgc cggcaggcag ttcgtccccg cccaccgcat cccagtagag ccgccggtag
    23701 atgacctcgg cctctgaacg ctggagtgcg cgcacctcgg ccctggtcac cgggcgcccg
    23761 cgccattcgg ccagcaccgc gcgggtgatg ccgagattgg tggcaccacc cggatcggcc
    23821 gggtgatcgg agtagccgcc ttcatgtcgc agggtgagag cgaggcagcg ggagaaattg
    23881 cgggcaacca tcagcaacct ttcacaacgg acagatcaga cagaaaggcg gaccgggatg
    23941 gtcgcgacgt gagccgtgcc gtcgccgatg ctctggctga tctggacaat acggaccgac
    24001 agggcagcct gaaccgagcc gaagtcggca atttcatcgg ccgccgggta gagaacctgc
    24061 ggcatattgg tggcaagacg gcgcttgagc gtggcgccgg aatagatatc gaccctgtag
    24121 gcctcgctgc cctccgacag gggcacctcc agaacgtccc acgaatcgcc atcgcgccgg
    24181 gtccgccgta tccaggtcag ttcgatgccg gccggcagcc gtctggcacg agcgaacacc
    24241 ggggccagcg gccttaatgc cgcatttcct gccggatggt cgaaggacag ggcgccaggg
    24301 tcggcatggt cgcgtccagc cgccgatatg cggaaatagg catgggagcc gatgtcctcg
    24361 ggggcggtat aaagcggctc cagcgcccca tcgatgagga ccgcgcggct gcccggcgga
    24421 gccagacggc tcgcctgtgc ctcggagccg gcaagcccgc gcagcaggcg tgccagacgc
    24481 caggtgcctg gaccgatcag ttcggcctgg gagaaagaca gcagttccca ggccgcatcc
    24541 gcaccctgaa cggcgagcac ttggctgcct gccagcgctc ccgcatcgcc catcgccgca
    24601 agcgccccgc cttccagccg gacggtcagg gccgccgccc tgtcccagcg ccacagcggg
    24661 ccgggaggca gggcggaaac cgtctcgccc agaatggccg cacgcccgat ggtgccgacc
    24721 ggggagaaac tcgctccgtc gctcgaccgc cagagtgtgt aggggccgac ccagggttcg
    24781 gcccgcacgc caatccaact cagcacgctt ggtgtcgccg gatcggccgg cagatcgacg
    24841 atcagcccca gcggtggtcc ggggatgcgc ggcggggtcc agtcggcgag cgcgttatcg
    24901 gttgagcgga ggccgaacac agctggctcg atgatccgca ggttgcagcg ccgctcgctg
    24961 ccatcggtga tgcgagtgat ctggcaggta tgcggctcgc ccgcgatgcg gacgaggtct
    25021 ccggtctcga gtgcgatcag accaggccgc aagccgattt cgagcgtctc gcgctggatc
    25081 caggcatcct gcagggcgat ttcggcgagg cgctgagcct cggcccggcg caggacagcc
    25141 gtggtttcgc tggcatcctc gcggcggctg gcggtttcca ggcgacgcga ggctacggca
    25201 gagcgcttgt actgctgctc ggcgtcggca aagccgatgg tgatctggcg gggaagttcg
    25261 gtttcctggg cgcgcgagcg caggatctcg acgcccccct tggccggcac gatatcatcg
    25321 cggtcgataa ggcgaggctc tccccgaccc cggccaaaaa agcaaagctc gccggaccgc
    25381 accccggcct cgacggcgaa gaggcccagc agcggctcaa gtgcagcgcg cgccgacatc
    25441 ggccggtcga tgatatagcc gtcgagcatg ccgtcgaccc cgcgacagca ggggagcggc
    25501 aaggcataat cggccgcaac agccctgatc aatcggtcga gcgcgagtga ttcgatccgc
    25561 ccggtgatcc agtggccggt cgcccagttg gcgccatcgc cccatacgct ctggagatgg
    25621 ggaaaggcgg gatatggccg cgcgtcccat gcccagacgt gaatgcgggc cggatccacc
    25681 atgcgaccgc catagaccgt cgagagaggg ttatgcgccg gctcgaaccc cggcagactg
    25741 gggtcgaacc gccgcatgac tgcttcgaga tagcgggcct gcacggcatc gtcgcgagcc
    25801 cctgaggaaa acggcggcag accactttcg gccgacttcg cgtcgggaaa cagattcggg
    25861 gcattggcac ccttgtcgac cgccggacag ccgacctcgg tcagccatat gggcttgcct
    25921 gacgcaaccc agcccgtggc tgcggtctcg gcgccggcaa cgcgctcgac atgcgcattt
    25981 gaccaccagc cgaccagatc cttggcgcgg aaggtccagg gcttgttgaa agcgccatcg
    26041 gtgatcggcg tgcgggtctg cgcgatgcgg tcggcatcgc tggcataata ccagtcgaag
    26101 gcctcgcctg ctcccaggcg gttcttcaga tagccgacat cctgcccgtc cggccagtct
    26161 gctgcatcga gatggctggt gccgtcgcgc cagtcggcga cggggggata atagtcgatg
    26221 ccgacgaagt cgatattggc gtcggaccac agcgggtcca gcggaaagcg aacctcggcg
    26281 ccgctggaca gcacatggct gccatattcg gtccagtcgg cggcataacc gatcttcacc
    26341 aaggggccga gcagggcgcg cacctcggcg gccagcagct tgagctctgc cactgccgga
    26401 tagacgccgg ccgccgagcg cacccgcgtc agcccgatca gttctgagcc gatgatgaag
    26461 gcatcgaccc cgccagccgc cttgcacagc gccgcctgat gcagcaccat gcgccgatag
    26521 ctccactctt ccggaccgga atagctgatc accccgccgg tgaccgagta atggctggcc
    26581 gaaaccgtgc cgaagaacgt ggccacctgc gccgcagccg ccgctgttcc atccggagag
    26641 ccggcgcggc ccggcgccgg gtggcaggtg atgcggccgc gccaggggaa cgccggctgg
    26701 ctggcgccgg cgttccaggg atcaggcaag gcattgccag aagcgatgtc catcatcacg
    26761 aacggataga acaccaccga aagcccgcgc gccttcagat cggcaatggc ggcccggatc
    26821 gacgcatccg acggtgttcc gccataagcg gcgcgaccgt tcacctcggt cacgacgcgg
    26881 gcggcgcccc gcgtcacatc ggcgacgctc cattgatccg ggccagcgga ggcaaaggga
    26941 tcaaggccgg acaatgtgcc tgcgctggtc gtcttggcgg cgcggtcgac cgccggggcg
    27001 atgcggcatt cacccgccct cagatccgtg ccaaaccagc ttgacaccag cgcgacgctg
    27061 gtggcgttcg gcatcagggc ctgcaatgcg tcgacagacg cctcgaagtc ggtggctgcg
    27121 gtcaactggt tgcggttttc gggctgggtt gcgccctggc cgatgttccg gctgaccacg
    27181 gtcgtgtgat agccgaattc ggtggcaccg gggatcatgt cgacgccgcg gatcatcccg
    27241 cccagaccgt cgaccgggcg caccacctcg aacgacagct gcggcaggcg attgccatag
    27301 tcggcaaggg gcagacgttc aaaaacgaca taagccgtgc cgcgataggc cggcgcagcg
    27361 ccagagcgcg caacgatcaa gggatcgggc atctgtgtct ggctaccgac ataggtgcgc
    27421 atggtcagac cattgagatc aagcggcttg ccatccgccc agatgcggcg gacgaaggcg
    27481 atcggtccct cgcacaacgc aatcgccaca ttggcgaaat aggcataggt ggtggtcacc
    27541 gtttgcggct gcctcgatcc ggaggacttg ccgcccgact tgccgctgcg ggttgtctcg
    27601 acctgttcct cgaaccgcgt cgcccagatg atctgcccgc cgacgcgcac ccggccatag
    27661 atgcgaggga tcggcgagcc ttcggatgcg gtgattccgt cgagctgctt gaggcgcggc
    27721 ccttcgacat agcgacgccc tccactgccc atcaggcgct gatcgatcat gccgccgatg
    27781 aggccgccgg ccgcctggcc gaccgctgcc agaatgggac cgcccagggc ggatccgagt
    27841 gccgaaccgg cagcttgcag aacgagcgtg gccatcagtc gatcactcct ggaaagcaga
    27901 aggcaaaggc gatgcgacgg cgccacaggc cgagcggaac ctcggcgacg gcagcgccat
    27961 catgggcatg gatgatgtga tccgcggcgg tgaggatcgc agcgtgtttg gccggcaggc
    28021 cggagcgcca gcggaacagc aggacgtcgc ctggcctggc cgcattgacg acaaccggga
    28081 ccagatgctg ccggcaggcc tgagcgaatg tctcgtcgaa accggcttcg gcccagtccg
    28141 gcgcataggg cggcggtgtt tccggctcag aaccgatcac ctcgcgccaa acgccgcgca
    28201 gaaggccgag gcagtcgcag ccgatgccgc gaagcgatgc ctgatgcaga tagggtgtgc
    28261 caatccaggc ccgggccgca gaaatgatgt ccgggcgcgt cagggccgtc atgggtcgag
    28321 cgggccgccg tcatgccccc cctcgcccgg cctggcgtat gtgaggatga agtccggcgt
    28381 cggaatgtgg ggaaagccgc ggaaattggc ggcgttgacg aatttcaggc cacaggtggc
    28441 aaaggacttg tcgcaacccg gcgtcaggcg caggctgtcg cccactgcca gggattgcgg
    28501 caaaggctcc cacaaggcga gcgtcccgcc cgctccatgc gtctgaatgg cgcgcgtcag
    28561 cccggcattc gctcctgtga gcaattctgc ccgcccgccg gcaaaatggg acgcggcagt
    28621 tgcggcgatg gcagcaaccg tcaggttcag gcggccatcg gtggccgtca cggccgccgt
    28681 gaccgcacgg gcggcgaggt cgattccaca agcggcatcg ccaagatcgg cggtgcaacc
    28741 ttgcgtataa agccggccct gctcctgatc atagcggtgc agcggccccc gcaactcggc
    28801 gacaaagctc tgatccatgc gccggatctc gccgatggtc gaaccatcca gcatcagccg
    28861 ttgcgaaaca tcggcccagt tgaccagcca ggtggttatt tcggcgtcat catagcgacc
    28921 accgatgatg tcggcctcgg tcagccgcgc atcggtcaag gccccgccga tctccccggt
    28981 ggaaacggca aggccgagct ggctttccac ttcactcggc gcaagaccgc tggtggccga
    29041 atagatctgg ccatcgaagg tcaggtcccg gtcatgatcg gtaaagccga aggccgttcc
    29101 gtcacgccgc accagcagcc agcagcggca cagggtggtg gctcctccgg ccagatggct
    29161 ggccagaggt tggggaatcg cacgcatggc aggctccgtc agatgaggat ttcgaccagc
    29221 gggatcttgg gaatggcccc ggcttcgaag gcttcgatgt cgatgtcgag catgtcactg
    29281 tcgaagcgca ctggaacatc ataggcaaag ccggcggtga ccgcttgccc cgcccccggc
    29341 aggtgcccgg cctgaaatgt gacaaggccg gtggtggaat cgaccgtgaa atcggttcct
    29401 gccaccttct cgatcccggc cacggcgatg cgcacactgc cggccaccgg cttgacgatc
    29461 gaccgcacgt agggagcgtg ctggccgcca tagcgtttga ccagctggaa cgtgctgcgc
    29521 gtgccggtgc cattgccgag gaactgatcg cccggcagga tcgcggcgcc gggaagcgcg
    29581 gaggaccagt ccagccgatc gcggaagcgg aagccgtgca ggcgcccgcg ccgctcctcg
    29641 aagaacgcaa tgatggccgc cagggtggcg aaggacttta ccccgtagcc agcgtcatat
    29701 cgccgccgcg aatgggccca gcgctggttg cgctcttcgt ggccggacgc catcgtcacg
    29761 acatcggtcc ggcgttcagg accaccgctg gcccgcagcg agacatccag cggaaaccga
    29821 acctcatgaa aagccatggc aggcacctct ttgacattga gccgagaata tgcatttcgg
    29881 cgggacagtt tcgtttccga gaaccagccg atgaggagtt gacatgagcc acacgccaca
    29941 cgaaatcgcc gccgaattcc ccgacaaggc cgagaagatc catcaactga agatgaccaa
    30001 cgcgcatttc gcccggctgg tggacgagta tcaccatgtg aaccggcaga ttcaccgcat
    30061 cgagaccgag gtcgagccga cctccgatca ggtgctcgag acgttcaaga agcagcgcct
    30121 gcatctgctg gatcaggtcg ccggcatgct gcgcgcctga ggcgcacgat tcaggtcgcg
    30181 cgccgaccgc gcgcgaccgc ccgtgccagt tgcgccgaga tctgactctc ggcccgggca
    30241 aagctggcaa tatccggggt ctggatgttg acgaccaccg gcgccgcccc tccccctccc
    30301 tgcgctgcaa caccaagccg cccgtcggcg ccgcgtgcca gcggcaggat
//
LOCUS       DAOMXG010000009        26818 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_1141_length_26818_cov_9.082950, whole genome shotgun sequence.
ACCESSION   DAOMXG010000009 DAOMXG010000000
VERSION     DAOMXG010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 26818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26818
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1141_length_26818_cov_9.082950"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(492..878)
                     /gene="pcaC"
                     /locus_tag="PLQ11_01280"
     CDS             complement(492..878)
                     /gene="pcaC"
                     /locus_tag="PLQ11_01280"
                     /EC_number="4.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003084072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047575 - 4-carboxymuconolactone
                     decarboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxymuconolactone decarboxylase"
                     /protein_id="HRK23564.1"
                     /translation="MSDDSSRHAAGMSVRRAVLGDAWVDRANARLTPFNREFQDLITR
                     YAWGEIWTRPGMDRTTRSCCVLSMMIALGAWEEFRLHVRAAFNNGLDRDAIKEVILQA
                     AIYCGVPKANHAIREAEAVFAEMDKD"
     gene            complement(875..1651)
                     /gene="pcaD"
                     /locus_tag="PLQ11_01285"
     CDS             complement(875..1651)
                     /gene="pcaD"
                     /locus_tag="PLQ11_01285"
                     /EC_number="3.1.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047570 - 3-oxoadipate enol-lactonase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipate enol-lactonase"
                     /protein_id="HRK23565.1"
                     /translation="MPMLTIGNEAFHYQLEGPKGAPVLMLSNSLGTDLTMWNTQAAAW
                     SQSFRVLRYDSRGHGRSTAPARPYSIAELGRDALAIMDALKLDRVHWCGLSKGGMVGQ
                     WLLTNARDRIGKSVLANTASRMGPPELWNTRIRTVTASGLGAIADTTRERWFSPKARA
                     SANPEIDRIIETFRSVPVAGYCGCCAAIRDMDQHWSIRSIANPVLVIIGKHDPATPPA
                     AGEHIARSIRGAKKKLLDAAHLSNIEQPAAFTRAVAEFLA"
     gene            complement(1670..3007)
                     /gene="pcaB"
                     /locus_tag="PLQ11_01290"
     CDS             complement(1670..3007)
                     /gene="pcaB"
                     /locus_tag="PLQ11_01290"
                     /EC_number="5.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047472 - 3-carboxy-cis,cis-muconate
                     cycloisomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxy-cis,cis-muconate cycloisomerase"
                     /protein_id="HRK23566.1"
                     /translation="MPGIYGELFSDAAMTDLFSDRATLQGMLDFEAALARDQASDGII
                     PESAVAPIAAAARADLYDIADIGRQATLAGNPAIPMVRLLTERVAAADPEAARWVHWG
                     ATSQDAIDTGRLIQIAGALRLIEARIDRLAAILARTADQHRRTVMAGRTLLQQALPVT
                     FGLKAAYWLDALLGHAAALSRIAPAASLQLGGAAGTLASLGDKGPALQERMMGFDLAV
                     PWHATRQALLRQGAELGLLTGTLGKIGRDVTLLMQTEIGEVSEAAAEGKGGSSTLPHK
                     RNPVQSVALTAIATRTPGLVATLLSAMVQEHERAAGAWQAEWQTLPDLFILAGAALAH
                     AVALIDGLGVDKARMRDNLDRTGGLILSERVAMALGEVMPRIEAKARVEAACAIAISE
                     RRPLRDVLAADAVIAAHLDGAALSRLFDPATYLGANDAVIDRVLALHRLHIAR"
     gene            3101..3550
                     /locus_tag="PLQ11_01295"
     CDS             3101..3550
                     /locus_tag="PLQ11_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HRK23567.1"
                     /translation="MAMTMTGTVDLPASRETAWAKLNDPEVLRVCIPGCQSLEGSIDE
                     GYRAEVKLKIGPVNAKFRGKVSLTDLDPPNGYRISGEGEGGIAGFAKGGATVRLAPIA
                     EGTTLTYEVEAHVGGKIAQLGARLINGVAKKLADQFFAKFAEAVKGG"
     gene            3595..5565
                     /gene="uvrC"
                     /locus_tag="PLQ11_01300"
     CDS             3595..5565
                     /gene="uvrC"
                     /locus_tag="PLQ11_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019995654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HRK23568.1"
                     /translation="MGRPNDDLPPDEPETDDTEEALPEPEEAGEASADAYGLGVDVIR
                     RFCKTLPNDPGVYRMLGADGGVLYVGKARSLKKRVASYTRGVGHSNRIQRMIAATASM
                     EFVTTETEAEALLLETNYIKQLKPRFNVLMRDDKSFPYILITGDHPAPQIVKHRGARN
                     RKGNYFGPFASAQAVGRTVNALQRAFLLRTCTDSYYDNRTRPCLLFQIKRCAAPCTGE
                     IGIADYAGLVDEARQFLSGRSVAVKQRIAAEMQAASDDLEFERAARYRDRLAALSAVQ
                     ASQDINPREVEEADVFAIHEEAGQFIVEVFFFRTFQNWGNRAFFPRADRSMAPAEVLD
                     AFVSQFYDERPAPRLVLTSHAIENAALIGEALSARAGRKVEVVHPRRGEKKDLVDHAG
                     RNAREALARKLADSAGQRKLLAALAEAFSLDKPPRRIDVFDNSHIMGTNAVGAMIVAG
                     EAGFMKTHYRTFNIKSEELTPGDDYGMMREVLTRRFSRLLKEAPRQQGAEEESFPPWP
                     DLVLIDGGEGQLAAVRGALAEIGVTDVPLVGVAKGPDRDAGREVFHVAGRPPFRLSPR
                     DPALYFIQRLRDEAHRFAIGTHRAKRKREMVANPLDEAPGVGPRRKRALLLHFGTAKA
                     VSRASLEDLMRTPGINEATARAVYDFFHEKQG"
     gene            5646..6248
                     /gene="pgsA"
                     /locus_tag="PLQ11_01305"
     CDS             5646..6248
                     /gene="pgsA"
                     /locus_tag="PLQ11_01305"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HRK23569.1"
                     /translation="MTSARRNPFNLPNILTYGRIAAVPVVVACLFWPKDDWVRWTALT
                     IYIVAGITDFLDGYLARIWEQQSSMGRMLDPVADKLLVGSVLLMLVHTGDIHSYSVWA
                     AIVILCREFLVSGLREFLAEVRVSVPVSRLAKWKTTAQLVALGFLIAGDAGDSLLPAG
                     WTINIGLALLWLAAVLTLYTGFDYFRAGIRHILEEDEKAS"
     gene            6245..6496
                     /gene="moaD"
                     /locus_tag="PLQ11_01310"
     CDS             6245..6496
                     /gene="moaD"
                     /locus_tag="PLQ11_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020090648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HRK23570.1"
                     /translation="MKLVYFAWVRERIGLSEEEVELPADVRTVADLLAWQQRRGERYA
                     HAFEKAPFIRVALDRSHARADQPVTGAREIAFFPPMTGG"
     gene            6500..6967
                     /locus_tag="PLQ11_01315"
     CDS             6500..6967
                     /locus_tag="PLQ11_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008192233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HRK23571.1"
                     /translation="MAVPLRIRVQAEDFDAAAEVRRLTEGRMDIGAVVTFTGLCRSEE
                     GRLAALELEHYPGMAEEEMTRVAEDAVRRWPLTGLSVIHRHGRIAPGAQIVLVVTTSA
                     HRRAAFEAAEFLMDFLKTRAPFWKKEHGVDGSEGGWVEARDEDDSAAARWTES"
     gene            complement(7016..7330)
                     /locus_tag="PLQ11_01320"
     CDS             complement(7016..7330)
                     /locus_tag="PLQ11_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23572.1"
                     /translation="MTPVKKLAAVAIASLTLATTFATSAEAQYRRHGWGPRPGPVYVN
                     RHRGFRGAPLAAGLLGSAVIGSMLMATRPAYAAPVYQDECYREYVGRDRYGRRLYQTV
                     CY"
     gene            7601..8089
                     /locus_tag="PLQ11_01325"
     CDS             7601..8089
                     /locus_tag="PLQ11_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HRK23573.1"
                     /translation="MATVSMTVNGKAVSADIDPRTLLVQFLRETLRLTGTHVGCDTSQ
                     CGACVVHVDGKAVKACTALALSCEGANVVTIEGLANGQQLHPMQEAFRDHHGLQCGFC
                     TPGMIMTAVDLVNRKGHDLDEATIREELEGNICRCTGYHNIVKAVDAGARAMGKGKKK
                     VA"
     gene            8152..10515
                     /locus_tag="PLQ11_01330"
     CDS             8152..10515
                     /locus_tag="PLQ11_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HRK23574.1"
                     /translation="MSATGIGASVKRKEDFRFITGKGNYTDDINRPGQTYAYFLRSPH
                     AHAAIKSIDLKAALKVPGVLAIYTGDDIAADKVGGLICGWMIHNKDGSPMAAGAHPAL
                     AQGKVRYVGDHVAVVIAETLDIAKDAAEKVKVNYDVLPALADVSAARKKGAALIHDNC
                     PGNRVYDWHLGDKAATDAAFKAARHVVKLDLVNNRLIPNAMEPRAAIGDYDAGNDALT
                     LYTTSQNPHVARLVLSAFIGIAPEHKLRVIAPDVGGGFGSKIFIYAEETVCLWAARKV
                     GRPVKWTGERIESFLSDAHGRDHVTHAEMAIDEGGKVTGVRVSTKANFGAYLSTFSSS
                     VPTYLYAPLLSGQYDIPAIYCEVEGIYTNTAPVDAYRGAGRPEATFVIERLMEIGARQ
                     TGQDPAAFRRKNFIRKFPHQTPVIMCYDAGDYNAALKKAMELADVKGFARRRRDSGRK
                     GKLRGLGYSAYIEACGIAPSQAVGSLGAGVGLWESAEVRVNPIGTVEILTGSHSHGQG
                     HETTFAQLVSDRLGIPIDNVAIVHGDTDKVQMGMGTYGSRSGAVGMTAIAKALDKIEA
                     KAKKVAAYVLEASETDIDFKNGQFSVKGTDKSIDFGSVALQAYIAHKFTGAQLEPGLK
                     EGAFYDPTNFTFPAGVHICEVEIDPETGVTKVERWTAVDDFGNVINPMIVEGQVHGGI
                     AQGIGQALCEGAVYGENGQLLTASYMDYHMPRADDLPSFKVDTVVTPCPSNPLGIKGC
                     GEAGAIAAPPAVINAITDALGHEDVRMPATAQEVWRAAHKARKQAAE"
     gene            10655..11458
                     /locus_tag="PLQ11_01335"
     CDS             10655..11458
                     /locus_tag="PLQ11_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HRK23575.1"
                     /translation="MYAFKYHRPASLRKAAALIAKHEDARLLAGGHTLLPTMKNRLAS
                     PSHIVDLGALADLAGVVRKGRNVVIGAMTCHADVATNADVIAALPGLAALAGNIGDPA
                     VRNRGTIGGSIANNDPAADYPAACLALGATIHTNKRKIAADEFFTGLFSTALDEGEII
                     TKVSFPTGGKFAYAKFSNPASRYAMVGVAVSKRGGEVRVAVTGAGADGVFRWAEAEKA
                     LAARFSAKALEGKSHPAKGLNSDIHASAEYRAHLIGVMARRAVAAATGK"
     gene            11817..12137
                     /locus_tag="PLQ11_01340"
     CDS             11817..12137
                     /locus_tag="PLQ11_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23576.1"
                     /translation="MLHKSMIPVAVLAACLMGASGQAMAKSGKNKALLTGGAVGLAAG
                     AVGGYMLGKSQSAPAAQAVDREPTGSTTYHRASARETCSVRSVELFDRKGNFVKSERM
                     RVCN"
     gene            complement(12204..13127)
                     /locus_tag="PLQ11_01345"
     CDS             complement(12204..13127)
                     /locus_tag="PLQ11_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRK23577.1"
                     /translation="MTDSPHPDAPPETTRQRMVAIVLVMGAVLMFTIIDASAKWLNPR
                     IGVLETVWLRYVVAFLWTLPFLNPWSVPGLLHSEKPVLQALRGFLLLASTALNFIALQ
                     YLQLAQTVSIAFMAPLMVSLLAGPILGEWAGPRRLAAIIVGFVGVLIMTRPGSGVFHP
                     AAILSLLSMTSYSIILLMTRRLQATDSPETTIFYTSLFGSLVLMPPALMLWSWPTGAL
                     EWLAIAMLGFGGALGHWMLIQAQRRTEAPVLAPFLYTQIIWMTLAGYLVFGDVPDRWT
                     LVGGTVVIASGLYLLHRERVRARERAAGRLP"
     gene            complement(13214..14395)
                     /locus_tag="PLQ11_01350"
     CDS             complement(13214..14395)
                     /locus_tag="PLQ11_01350"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HRK23578.1"
                     /translation="MASQRHAVIAGAGIGGLTAALTLARAGWTVDVFEQAHKLEPVGA
                     GVQLGPNATRILYDLGLEAALAETAVATEQLIVRTGLDGRRRNGMRHTGEAVRRWNAP
                     FLVIHRGDLQMTLLAAASATPGVTIMLGQRLEDISERTGRTMASFSKYGEVTEIDADL
                     IVGADGVWSKARSHVGLTGPTNFSGSVAWRTVVPAEAVPEAMRARNTNLWLGPGAHVV
                     HYPVQSGRTINIVAVVDDGWRERGWSESGDISWINRRFIDWHKDLRTLIGSAEHWLRW
                     SLFDRPPEPKWSRGIITLLGDAAHPMLPFLAQGAAQAIEDAAALAAALADTPDVAAAL
                     RNYERRRIPRTARVQKLSHRQRRIYHASGPLATARDIAMGLMGEKGLADRFDWLYRED
                     P"
     gene            complement(14408..14647)
                     /locus_tag="PLQ11_01355"
     CDS             complement(14408..14647)
                     /locus_tag="PLQ11_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HRK23579.1"
                     /translation="MAHHAIPHFHNDSGVAVIEIAAREFMCIGAKPPFDHPHVFLDMG
                     ADNEIICPYCSTLFRYSKALHGTQSQPPSLWTDAA"
     gene            14822..15598
                     /locus_tag="PLQ11_01360"
     CDS             14822..15598
                     /locus_tag="PLQ11_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRK23580.1"
                     /translation="MHRFVHKGVGLNYIDVPPHEGSGPAVLLIHGFASNHSVNWVATQ
                     WVRSLTHAGYRVIALDNRGHGLSDKLYDPAEYTTFLMADDAAALLGHLGIEGAGVIGY
                     SMGARIAAFLTRAHPRLVRVLAMGGLGYRLVDGIGLPQSIAEALEAPGLNAIEDPMGR
                     MFRAFAEMTGSDLRALAACIRGSRQTMPLEHVRMIATPTIVAVGTNDPIAGDARRLTD
                     VMQNAVPFDIEGRDHNVAVGDITHRKAVIAFFDNHLKKEP"
     gene            16017..16844
                     /gene="cysE"
                     /locus_tag="PLQ11_01365"
     CDS             16017..16844
                     /gene="cysE"
                     /locus_tag="PLQ11_01365"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HRK23581.1"
                     /translation="MSANVHGLKANVAPVDPVWRRVRLEAEAVLKAEPEMAGLVLSNI
                     LHQPSLEAAMAQRVSSRLDHDDLSGALIRQAFEEMFAAQPDLGQWLRMDIMAVLDRDP
                     ACDRLIEPILYFKGFQALQTHRLTHWLRSKGRGDFALYLQSRSSNVFQVDINPAARIG
                     KGVFFDHATGLVVGETAVIEDNVSILQGVTLGGTGKEHGDRHPKIRHGVLIGAGAKIL
                     GNIEVGHCARVAAGSVVLQDVPHNKTVAGVPARIVGDSGCAEPSRLMDQILHGDPGI"
     gene            16919..17128
                     /locus_tag="PLQ11_01370"
     CDS             16919..17128
                     /locus_tag="PLQ11_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3126 family protein"
                     /protein_id="HRK23582.1"
                     /translation="MEKPDIQRVESYLRRTFNNANIRLMARARKKDSAEVFIGEEFIG
                     LVHEDKEDGDLSYNFTMAILDTDLE"
     gene            complement(17135..17797)
                     /locus_tag="PLQ11_01375"
     CDS             complement(17135..17797)
                     /locus_tag="PLQ11_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23583.1"
                     /translation="MVRLLVISCIAGLAAIGGMLARRIYVETRPPSGHAVFHVGSHRL
                     TVPRAMVRNLDLRDGGAVNRLDLVLNWPDLSGAAGNLGGKTAPALVFLSLEDSSALAK
                     SRDDIDPAERPVELYARFLEADAVTGPAGLVQRRFSRGSPYEGEDLFMATPDERQFSA
                     RCPRGAAIPQPAEQCLWQTRQAGLELHVRFAPALIQDWQRLTSAIRALASVLRTQPPA
                     RP"
     gene            18050..18403
                     /locus_tag="PLQ11_01380"
     CDS             18050..18403
                     /locus_tag="PLQ11_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23584.1"
                     /translation="MGWDALSALATMLAGLVFAGGLIEAVAGFTRQRIGFDLVWRSVR
                     LGWLALPFLIMAGPAIVLRETALRRTGPGWNGALFAVGLVVALAWALASGHVLLSIIR
                     ASEPLLVTGQVRLLN"
     gene            complement(18570..19118)
                     /locus_tag="PLQ11_01385"
     CDS             complement(18570..19118)
                     /locus_tag="PLQ11_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like cysteine peptidase"
                     /protein_id="HRK23585.1"
                     /translation="MAMHAPPAGAETTPPIGWVEFCERSGRECMVEALPAEIIPLDET
                     SWREIVRINAAVNREITPVTDMENHGVIELWSYPTDGKGDCEDFVLEKRKRLMRAGFP
                     RQALLITVVRDNKGDGHAVLTVKTDHGDFILDNQASKVLAWHETGYRFIKRQSQETPN
                     RWVSLGGIDTATVAVSRRPRMR"
     gene            complement(19805..20401)
                     /locus_tag="PLQ11_01390"
     CDS             complement(19805..20401)
                     /locus_tag="PLQ11_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRK23586.1"
                     /translation="MLLSGSKLAINPVEQRRFQRVEVILHGRFMLPDHSEHECVTIDM
                     SPGGARLRTRAHGKVNDRVIAYLETVGRIEGQIARLTEDGFAMTINATLRRQDKLAAQ
                     LTWLANRHELGLPEDRRHERYVPRNPHGRLVTAGGAEVMVRLIDVSLSGAALTTDLSF
                     TRGDLVMLNNTPSKVVRVFERGIAVEFSRPPAPGAFEV"
     gene            complement(20503..21099)
                     /locus_tag="PLQ11_01395"
     CDS             complement(20503..21099)
                     /locus_tag="PLQ11_01395"
                     /inference="COORDINATES: protein motif:HMM:NF018960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRK23587.1"
                     /translation="MRHPVSKHLFAYWRRLRGPRPAPERADINPADLGRLLPHMFVLE
                     GTGDGAFRIRIAGSRLCALFDTELRGTDLQALMAPEAVDDLRDILAIVATDLNPVIAG
                     ASVLLMNEAPAAAELLLLPLLHRSQPGERLLGCLTFGAELPPPRVACLGIDIISFRVI
                     DDSETRFLHPPEPELPIHLRGMAERRGHLTVLEGGQHP"
     gene            21469..21897
                     /locus_tag="PLQ11_01400"
     CDS             21469..21897
                     /locus_tag="PLQ11_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007197488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRK23588.1"
                     /translation="MIVAHILNQKGREVATVEPGQPVSDVVSVLGTRSYGALVVSGSD
                     RRIQGIISERDVVKLLARSGAGALSEPVSKHMTAKVVTCAADDTIDQVMQAMTKGRFR
                     HMPVEHDGKLNGIISIGDVVKARLEQLANESEALRAYING"
     gene            complement(22267..23241)
                     /locus_tag="PLQ11_01405"
     CDS             complement(22267..23241)
                     /locus_tag="PLQ11_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRK23589.1"
                     /translation="MSTPLPEPKPQPKGPVIGLALGGGVARGWAHIGILRVLEREGIR
                     PKIVTGTSIGAVVGGCWATGYLDQLELFARRLTKRGIFNLMDFSLSGSGLVGGRRLRE
                     MLDEALGDRAIENLPTRFAAVATEYGTGHEIWLKRGHLGTAVRASYAIPGVFEPVKVN
                     DKWLLDGALVNPVPVTTARALGATMVIAVNLNNEHFGRSTVISDRVAEPPPAETPDAT
                     GRSGLFRPVKDAANAVSYYFNGRPSTPSGTPSIASVMIETINITQDRIARSRLAGDPP
                     DVMIAPKLARIGLFEFHRADEAIALGAEAAERMLPDLREVFDALAARQ"
     gene            complement(23400..23825)
                     /gene="hisI"
                     /locus_tag="PLQ11_01410"
     CDS             complement(23400..23825)
                     /gene="hisI"
                     /locus_tag="PLQ11_01410"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015950928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HRK23590.1"
                     /translation="MWTPKTPFAPPGDKVELEQGTQLTPKFDADGFIPVIAQEAGSGQ
                     VLMLAYMNAEALRRTIETGEAWYWSRSRKAFWRKGETSGNSQTVIAIATDCDQDTLLV
                     TVRQTGPACHTGRKSCFYRTLSRAGDGTLTLSFTETSGG"
     gene            23920..24363
                     /locus_tag="PLQ11_01415"
     CDS             23920..24363
                     /locus_tag="PLQ11_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003609453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HRK23591.1"
                     /translation="MLNDVYNQRILELAGNIPRLGRLAAPMASATAHSRLCGSTVTVD
                     LAIRDGKVSDFAHDVKACALGQASSSIMARNIVGSTPAELRSVRETVRRMLKENGAPP
                     ADGKWTDLRVLEPVRDYKARHASTMLTFDAVVEALDAIEAGGKGR"
     gene            complement(24383..25597)
                     /locus_tag="PLQ11_01420"
     CDS             complement(24383..25597)
                     /locus_tag="PLQ11_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23592.1"
                     /translation="MAAQGRKPVATPATSPEPMTFRLVSSNPDAPRERQIRWVTASGA
                     ITNDTPRRFDAFLKDNPLDETLTIYFDSKGGSILGGLELGERLRTVRARVAIGRSTAV
                     DNGDAEGAAGPARLPRHQLVPNLGLCFSSCAYAFLGGRHRFVPIGASYGVHMFWPSDK
                     MEDLFGRRYGFEEIERAQRISARLGTYIQKMGVDLKLLELAASSPPRNSIRRLSPREI
                     VELKVGSVVTGVPLFSGEGAWGLATADRTVSLSTGGLARRPGKPDLRYVLELSCHPDT
                     GFLRARFEQSPVVAPTEAAPVTLSRAVLASGETSATLAASGKDIIALPARFPRLAATN
                     AGHWIGKSGVIVDDPLRNLLARPADGLRVMLDEDENRRSEIVIPTASFASAGRAFLNA
                     CDGLRKGDKPVE"
     gene            complement(25699..26526)
                     /locus_tag="PLQ11_01425"
     CDS             complement(25699..26526)
                     /locus_tag="PLQ11_01425"
                     /inference="COORDINATES: protein motif:HMM:NF018352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HRK23593.1"
                     /translation="MLRWVACFLAVLAIFAGHRPAAAADTTRVAFEPARIDGYIVTVK
                     ANAFASPKWQGAGVMSAIAYPTVSFRKVGEAPKFSSPDDGIGFGLLDNKVFTAGPVFR
                     YVGGRYDGSDKDLRGIHDARWTLESGLFADAWITPNLRARAEIRRGFREKDGFAANLG
                     LDLVNSFNSWTVALGPRLAFGDGSYMRNHFGVTARDAALNPRVYKYRAKSGMKSVGIY
                     ASATYKFSDTWSATLHGGYDRLVRSAANSPITARLGSTNQYTVGLTLSYSFPMKGFW"
     gene            26617..>26818
                     /gene="yidD"
                     /locus_tag="PLQ11_01430"
     CDS             26617..>26818
                     /gene="yidD"
                     /locus_tag="PLQ11_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010660102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HRK23594.1"
                     /translation="MKDEGPGRLSRLMALPLIGLVRIYRYSLSSLMGRTCRYLPTCSE
                     YTEEALRRHGGWAGGWIGLARIC"
BASE COUNT         4895 a         8218 c         9078 g         4627 t
ORIGIN      
        1 tttgtaagtg gctcgctgcg cgagcatccc tcgcactcgc tcggcgtcgc ttcgcaggtt
       61 tgtaagtggc tcgctgcgcg agcatccctc gcactcgctc ggcgtcgctt cgcaggtttg
      121 taagtggctc gctgcgcgag catccctcgc actcgctcgg cgtcgcttcg ctcctatggc
      181 agggcgctgt cgtcttccct cccccttgcg gggagggtgg tccaaacatc gcagacaagt
      241 ctacgcagtc tgcgtagact tgtctgcgtg tggaggaccg ggtgggggtg gtgctgcatg
      301 acatgcggag cccggatcat ccacccccat ccccaaccct tccccgcaag ggggaaggga
      361 gaggcctcat accaaaagtc attgaaaatg actctttggt gcaactcaaa aatttctcat
      421 ctcgttgata atagtgagaa atttttgaaa cgaaccaagt cgcggcgagt tcctcgcctt
      481 gcgacttggt atcagtcctt gtccatctcg gcgaacaccg cctcggcctc cctgatggca
      541 tggttggcct tgggcacgcc gcagtagatg gcggcctgca ggatcacttc cttgatggcg
      601 tcgcggtcga ggccgttgtt gaaggcggcg cggacatgca ggcggaattc ctcccacgca
      661 cccagcgcga tcatcatcga cagcacgcag caggagcgcg tcgtgcggtc catgccgggc
      721 cgggtccaga tctcgcccca ggcatagcgt gtgatgagat cctgaaattc gcggttgaac
      781 ggggtcagcc tggcattggc gcggtcgacc caggcatcgc cgaggacggc acggcgcacg
      841 ctcattccgg cggcgtggcg ggacgaatca tcgctcatgc gagaaactcc gcgaccgcgc
      901 gggtgaaggc ggcgggctgt tcaatgttgg agaggtgagc ggcatccagc agcttcttct
      961 ttgcgccgcg gattgagcgg gcgatatgct caccggcagc gggcggggtg gccgggtcgt
     1021 gcttgccgat gatgaccaga accggattgg cgatcgagcg gatcgaccaa tgctggtcca
     1081 tgtcgcggat ggccgcgcag caaccgcaat agccggcgac cggcaccgaa cggaacgttt
     1141 cgatgatgcg gtcgatctcc ggattggcag aggcgcgggc tttcggcgag aaccagcgtt
     1201 cgcgcgtggt atcggcgatg gcgcccaagc cgcttgccgt cacggtgcgg atgcgggtgt
     1261 tccacagctc cggcggtccc atgcgcgagg cggtgttggc gagcaccgac ttgccgattc
     1321 gatcccttgc gttggtgagc agccattgcc cgaccatgcc gcccttggac aggccgcacc
     1381 aatgcaccct gtcgagtttg agcgcatcca tgatcgccag cgcgtcgcgg ccgagttcgg
     1441 cgatggaata gggccgggcc ggggcggtgc tgcggccgtg gccgcggctg tcatagcgca
     1501 acacgcggaa gctctgcgac catgcggcgg cctgggtgtt ccacatcgtc agatcggtgc
     1561 cgagcgaatt ggacagcatc agcaccgggg cgcctttcgg cccctccagc tgatagtgaa
     1621 acgcttcgtt cccgattgtc agcatcggca tcggcgggcc tccttccctt cagcgggcaa
     1681 tatgcaggcg gtgcagcgcc agcacgcggt cgatgaccgc atcattggcg ccaagatagg
     1741 tggcgggatc gaacagccgg ctgagcgccg ccccgtcgag atgggcggca atgacggcat
     1801 ccgctgccag cacatcgcgc agcgggcgcc gttcgctgat ggcgatcgca caggccgcct
     1861 cgacgcgggc tttggcctcg atgcgcggca tgacctcgcc gagcgccatg gcgacccgtt
     1921 cggacaggat caggccgccg gttcggtcga gattgtcgcg catccgtgcc ttgtcgacgc
     1981 cgagtccgtc gatcagcgcc acggcatggg ccagtgcggc gccggcgagg atgaacagat
     2041 cgggcagggt ctgccattcc gcctgccagg cgccggcagc gcgctcatgc tcctgcacca
     2101 tggcagagag cagggtggcg acgagccccg gggtgcgggt ggcgatggcg gtcagggcaa
     2161 cgctctgcac cggattgcgc ttgtgcggga gggtggacga gccgcccttg ccctcggcgg
     2221 cggcttcgct cacctcgccg atctcggtct gcatcagcag cgtgacatcg cggccgatct
     2281 tgccgagcgt gccggtgagc aggccgagtt cggccccttg gcgcagcagc gcctggcggg
     2341 tcgcatgcca cggcacggcg aggtcaaacc ccatcatccg ctcctgcagc gccgggccct
     2401 tgtccccgag cgaggcgagt gtgcccgccg cgccgccgag ctggaggctg gcggccgggg
     2461 cgatacggga gagcgcggcg gcatgaccga gcagcgcatc cagccaatag gccgctttca
     2521 ggccgaacgt caccggcagc gcctgctgca gcagcgtgcg gccggccatg acggtgcggc
     2581 gatgctggtc tgcggtgcgg gcgaggatgg cggcgagccg gtcgattcgc gcctcgatca
     2641 atcgcagcgc tccggcgatc tggatcagcc gcccggtgtc gatggcatcc tggctggtgg
     2701 cgccccagtg cacccagcgg gcggcttccg gatcggcggc tgccacccgc tcggtcagca
     2761 gccgcaccat gggaatcgcc ggattgcccg ccaatgttgc ctgccggccg atgtcggcga
     2821 tgtcgtagag gtcggcgcgg gcggcggcgg cgatcggcgc aaccgcgctt tcgggaatga
     2881 ttccgtcgct ggcctgatcg cgcgccaggg ctgcctcgaa atcgagcatg ccctgcagcg
     2941 tcgcccggtc ggaaaacaga tccgtcatcg cggcgtcgga aaacagttcg ccataaatgc
     3001 ccggcattgc cattcgcctc cttccccaag gtgcaggttt ggtccagact agcgaccaga
     3061 tgcggcgccg caagccgctt tgccactgag gggacgcacc atggcaatga cgatgaccgg
     3121 aacggtggat ctgccggcca gccgcgagac cgcttgggcg aagctcaacg atccggaggt
     3181 gctgcgggtc tgcattcccg gatgccagag ccttgaaggg tcgatcgatg aaggttaccg
     3241 ggccgaggtg aagctgaaga tcgggccggt caatgccaag ttccgcggca aagtcagcct
     3301 gaccgatctc gatccgccga acggctaccg gatcagcggc gaaggcgagg ggggaattgc
     3361 cggcttcgcc aagggcggtg caacggtcag gctggcgccc atcgcggagg gaaccacgct
     3421 cacctacgag gtcgaggcgc atgtcggcgg aaaaatcgcc cagctcggcg cgcggttgat
     3481 caatggcgtc gccaagaaac tggccgatca gttcttcgcc aaattcgccg aggcggtgaa
     3541 gggcggctga gttcgcgctt ccgcagggaa aacaaaacac ctatctctgc tgccatggga
     3601 cgcccgaacg acgatctgcc gcccgacgag ccggaaaccg acgacaccga ggaggccctg
     3661 ccggagccgg aggaggccgg cgaggccagc gccgatgcct atgggcttgg cgtcgacgtc
     3721 atccggcgct tctgcaaaac gctccccaat gatcccggcg tctatcgcat gcttggggcc
     3781 gatggcggcg tgctctatgt cggcaaggca cgctcgctga aaaagcgcgt cgccagctac
     3841 acgcgcggtg ttggccattc caaccgcatc cagcggatga tcgccgccac cgcctcgatg
     3901 gagttcgtca ccaccgagac ggaagccgag gcgctgctgc tcgaaaccaa ctatatcaag
     3961 cagttgaagc cgcgcttcaa tgtgctgatg cgcgacgaca agtcgtttcc ctatatcctg
     4021 atcaccgggg atcatccggc gccgcagatc gtcaagcatc gcggcgcgcg caaccgcaag
     4081 ggcaattact tcgggccgtt cgcctcggcc caggcggtgg ggcggacggt caatgccctc
     4141 cagcgcgcct tcctgctgcg cacctgcacc gacagctact atgacaatcg cacgcgcccc
     4201 tgcctgctgt tccagatcaa gcgctgcgcc gccccctgca ccggcgagat cggcatcgcc
     4261 gattatgccg ggctggttga tgaggcgcgg cagttcctgt cggggcggtc agtggcggtc
     4321 aagcaacgga tagctgcgga aatgcaggcg gcctccgatg acctcgaatt cgagcgcgcc
     4381 gcacgctatc gcgaccggct ggcggcgctc tcggcggtgc aggcaagcca ggacatcaac
     4441 ccgcgcgagg tggaggaggc ggatgtgttc gccatccatg aggaagccgg gcagttcatc
     4501 gtcgaggtgt tcttcttccg caccttccag aactggggca accgtgcctt ctttccgcgg
     4561 gccgaccggt cgatggcgcc ggccgaagtg ctcgatgcct tcgtcagcca gttctacgac
     4621 gagcggccgg caccaaggct ggtgctcacc agccacgcca tcgagaatgc cgccctgatc
     4681 ggcgaagcgt tgtcggcgcg ggcagggcgc aaggtcgagg tcgtccatcc caggcgtggc
     4741 gagaagaagg atcttgtcga ccatgccgga cgcaatgccc gcgaagcgct ggcgcgcaag
     4801 ctcgccgaca gtgccggcca gcgcaagctg ctggccgccc tggccgaggc gttcagcctc
     4861 gacaagccgc cgcgccgaat cgacgtgttc gacaattccc acatcatggg caccaacgct
     4921 gtcggcgcga tgatcgttgc cggcgaggcg gggttcatga aaacccatta ccgcaccttc
     4981 aacatcaaat ccgaggagct gacgcccggc gacgattacg ggatgatgcg cgaggtgctg
     5041 acgcggcggt tttcacgctt gctgaaggag gccccgcgcc agcagggggc ggaggaggag
     5101 agctttccgc cttggccgga tctggtgctg atcgacggcg gcgagggcca gcttgcggcg
     5161 gtgcgcggcg cgctggcgga gatcggggtg accgacgtgc cgttggtcgg tgtcgccaag
     5221 ggaccggatc gtgatgcggg gcgggaagtg ttccatgtgg ccgggcggcc gccgttccgg
     5281 ctcagcccgc gcgatccggc gctgtatttc atccagcggc tgcgcgacga ggcgcatcga
     5341 ttcgccatcg gcacgcatcg ggccaagcgc aagcgcgaga tggtcgcaaa tccgctcgat
     5401 gaagcgcccg gcgtcggccc ccgccgcaag cgggcgctgc tgctgcattt cggcacggcc
     5461 aaggctgtca gccgggcgtc gctcgaggat ctgatgcgca cgccgggcat caacgaagcg
     5521 acggcgcgcg cggtctacga tttcttccat gaaaaacaag gttgagaccg gcggttggcg
     5581 gttgacggct ttggcgccgg tatgctccgg tgccgccatc tagtctccga tccaacccag
     5641 ctacgatgac ctcggcacgc cgcaatccgt tcaacctgcc gaacattctc acctacggcc
     5701 gcatcgccgc ggtgccggtg gtggtggcat gtctgttctg gccgaaggat gactgggtgc
     5761 gctggaccgc gctcactatc tatatcgtgg ccggcatcac cgactttctc gatggctatc
     5821 tggccagaat ctgggaacag caatcgtcca tggggcggat gctcgatccg gtggccgaca
     5881 agctgctggt cggctcggtg ctgctgatgc tggtccatac cggcgatatc catagctatt
     5941 cggtctgggc ggccattgtc atcctgtgcc gcgagtttct ggtgtcggga ttgcgcgaat
     6001 tcctcgccga ggtccgcgtt tcggtgccgg tatcgcggct ggccaagtgg aaaaccacgg
     6061 cgcaactggt ggcgctgggc tttctgatcg ccggagatgc aggcgacagc ctgctgccgg
     6121 ccggatggac gatcaacatc gggctggcgc tgctatggct tgccgctgtt ctgacactgt
     6181 atacgggctt tgattatttc agggcgggca tccgccatat tctggaagag gatgaaaagg
     6241 cgtcatgaag ctcgtctatt tcgcctgggt gcgcgagcgg atcggcctgt ccgaggaaga
     6301 ggttgaactg cccgccgacg tgcggacggt tgcagacctg ctggcctggc agcagaggcg
     6361 gggcgagcgc tatgcccatg ccttcgaaaa ggcgccgttc attcgcgtgg cgctcgatcg
     6421 cagccatgcc agggccgatc agccggtgac cggcgcgcgg gagatcgcct ttttcccgcc
     6481 gatgaccggg ggctgagcga tggccgtgcc gcttcgcatc cgtgtgcagg cggaggattt
     6541 cgacgccgca gccgaagtgc ggcgcctcac cgaagggcgg atggatatcg gcgcggttgt
     6601 gaccttcacc ggcctttgcc gcagcgagga agggcggctc gccgcgctcg aactcgaaca
     6661 ttatccgggc atggccgaag aggaaatgac acgggtcgcg gaggatgcgg tgcggcgctg
     6721 gccgctgacc gggctgagcg tgatccaccg ccacgggcga atcgccccag gcgcccagat
     6781 cgtgctggtg gtgacaacct cggcgcatcg gcgggctgcg ttcgaggcgg ctgaatttct
     6841 catggacttt ctcaagacgc gcgcgccgtt ctggaagaag gagcacggcg ttgacgggtc
     6901 cgagggcgga tgggtcgaag cgcgcgacga ggacgacagc gccgccgcgc gctggacaga
     6961 atcctgaacg gccccgccgg agcggggccg catgcagcaa tccagttgtg ccggatcagt
     7021 agcagacggt ctgatacagg cgccggccgt aacggtcgcg gccgacatat tcgcggtagc
     7081 attcgtcctg atagaccggc gcggcatagg ccgggcgggt tgccatcagc atcgagccga
     7141 tcaccgcgct gccgagcaga ccggccgcca gcggagcgcc gcggaaaccg cggtgacggt
     7201 tcacatagac cgggccgggc cgcgggcccc agccatggcg gcgatactgc gcctcggcgc
     7261 tggtggcgaa agtggtggcg agcgtgagcg aggcaatcgc aaccgcagca agcttcttca
     7321 caggggtcat ggcatcctcc tcagggccca tcacaggcag aaccgaacac tcggcctgac
     7381 gttgatggtg agttaaccat cggctagctg aacggaggct gagtttttcg tttgtcattg
     7441 cgacagaggg ccgggttggc tcttgaccgg tttcaagggg cgggccaatc tcgctgacca
     7501 acggcgtgtg cgtcccttca ctggcaggcg tgatgccgaa agacacgtgc cggccacgct
     7561 ttgggcagac cgggccggac gcatcgagga ggaacaccct atggccactg tctcgatgac
     7621 ggtgaatgga aaagctgtat ccgcagacat cgatccgcgc acgctgctgg tgcagttcct
     7681 gcgcgagacg ctgaggctga cgggcacgca tgtcggctgc gacaccagcc agtgcggcgc
     7741 ctgcgtcgtt catgtcgatg gcaaggcggt gaaggcatgc accgcgctgg cgctgtcctg
     7801 cgagggcgcc aatgtcgtca ccatcgaggg gctggccaac gggcagcagt tgcacccgat
     7861 gcaggaggcg ttccgcgatc atcacgggtt gcaatgcgga ttctgcacgc cggggatgat
     7921 catgacagcg gttgatctgg tcaatcgcaa ggggcacgac ctcgacgagg cgaccatccg
     7981 cgaggaactc gaaggcaata tctgccgctg caccggctac cacaacatcg tcaaggcggt
     8041 cgacgccggg gcgcgggcga tgggcaaggg caagaagaag gtcgcctgag cccgcctgcc
     8101 atcgtgcctt atcagtctga tccagccatt catccctcgg gaggggacgt catgagtgcc
     8161 acaggcatcg gagcttccgt caaacgcaag gaagacttcc gcttcatcac cggcaagggc
     8221 aattacaccg acgatatcaa ccgccccggc cagacctacg cctatttcct gcgctcgccg
     8281 catgcccatg cggcgatcaa gtccatcgac ctgaaggcag cgctgaaagt gccgggcgtg
     8341 ctggcgatct ataccggcga tgacatcgct gccgacaagg tcggcgggct gatctgtggc
     8401 tggatgatcc acaacaagga tggctcgccc atggccgccg gcgcgcatcc ggcgctggcg
     8461 cagggcaagg tgcgctatgt cggcgaccat gtggcggtcg tcatcgccga gacgctcgac
     8521 atcgccaagg acgcggccga gaaggtcaag gtcaactatg atgtgctgcc ggcgctggcg
     8581 gatgtgtccg ccgcgcgcaa gaagggcgcg gcgctcattc atgacaattg ccccggcaac
     8641 cgcgtctatg actggcatct cggcgacaag gcagcgaccg atgcggcgtt caaggctgcc
     8701 aggcatgtcg tcaagctcga tctggtcaac aatcggctga tccccaatgc gatggagccg
     8761 cgcgccgcaa tcggcgacta tgacgcgggt aacgatgcgc tgacgctcta taccaccagc
     8821 cagaatccgc atgtggcgcg gctggtgctg tcggcattca tcggcatcgc gcccgagcac
     8881 aagttgcggg tgatcgcgcc tgatgtcggc ggcgggttcg gctcgaagat tttcatctat
     8941 gccgaggaga cggtgtgcct gtgggcggcg cgcaaggtcg gccggccggt gaaatggacg
     9001 ggcgagcgga tcgaatcatt cctgtccgac gcccatggcc gcgatcatgt cacccatgcc
     9061 gagatggcga tcgacgaagg cggcaaggtg accggcgtgc gggtttcgac aaaggccaat
     9121 ttcggcgcgt atctgtcgac tttctcgtcg tcggtgccga cctacctcta cgcgccgctg
     9181 ctgtcgggcc agtatgatat tccggcgatc tattgcgagg tggagggcat ctacaccaac
     9241 accgcgccgg tcgatgccta tcgcggcgcc ggccgcccgg aggccacttt tgtcatcgag
     9301 cggctgatgg aaatcggcgc gcgccagacc gggcaggacc cggcggcgtt ccggcgcaag
     9361 aacttcatcc gcaagttccc gcaccagacg ccggtgatca tgtgctacga cgccggcgac
     9421 tataacgccg cgctgaaaaa ggctatggaa ctggccgacg tgaagggctt tgccaggcgc
     9481 cggcgcgaca gcgggcgcaa gggcaagctg cgcggcctgg gctattcggc ctatatcgaa
     9541 gcctgcggca tcgcgcccag ccaggcggtt ggttcgctcg gcgccggcgt cggcctgtgg
     9601 gaatcggccg aggtgcgcgt caatccgatc ggcacggtgg aaatcctcac cggatcgcac
     9661 agtcacgggc aggggcatga gaccaccttt gcccagcttg tctccgaccg gctcggcatt
     9721 ccgatcgaca atgtggcgat cgtccatggc gataccgaca aggtgcagat gggcatgggc
     9781 acctacggct cgcgttccgg cgcggtcggc atgacggcga ttgccaaggc gctcgacaag
     9841 atcgaggcaa aagccaagaa ggtcgccgcc tatgtgctgg aagcgtccga gaccgatatc
     9901 gacttcaaga acgggcagtt cagcgtcaag ggcaccgaca agagcatcga cttcggatcg
     9961 gtggcgttgc aggcctatat cgcccacaag ttcaccggag cgcagcttga gccggggctg
    10021 aaggaggggg cgttctatga tcccaccaac ttcaccttcc cggccggcgt gcatatctgc
    10081 gaggtcgaga tcgaccccga gaccggtgtg acgaaagtgg agcggtggac cgcggtcgac
    10141 gatttcggca atgtcatcaa cccgatgatc gtcgaaggcc aggtgcatgg cggcatcgcc
    10201 cagggcatcg ggcaggcgct gtgcgaaggg gccgtctatg gcgagaacgg ccagctcctg
    10261 acggcgagct acatggacta ccacatgccg cgtgccgacg acctgccgtc gttcaaggtg
    10321 gatacggtgg tcactccgtg cccgtcgaac ccgctcggca tcaagggctg cggcgaggcc
    10381 ggagcgatcg cggcgccgcc ggcggtcatc aatgccatta ccgatgcact cggccatgag
    10441 gatgttcgca tgccggcgac cgcgcaggaa gtgtggcgtg cggcgcacaa ggcacgcaag
    10501 caggcggcgg aataattgtt tcttctcccc gccccgcggg gagaaggtgg ccgaaggccg
    10561 gatgaggggt caggcgataa gcccctcacc ctagccctct ccccgccagc ggggagaggg
    10621 gatcgcgcca gggatcttgc aggagcactt ttccatgtat gctttcaaat atcaccgtcc
    10681 ggcctccctc aggaaagcgg cggcgctgat cgccaagcat gaggacgccc ggttgctggc
    10741 aggcggccac acgctgctgc cgacgatgaa gaaccggctg gcgagcccga gccatatcgt
    10801 cgatctcggc gctctggccg acctcgccgg agtggtgcgc aaagggcgca atgtggtcat
    10861 cggcgccatg acatgtcatg ccgatgtggc gaccaatgcc gatgtgatcg cggcgctgcc
    10921 gggcctggcg gcgctggccg gcaatatcgg cgatccggcg gtgcgcaatc gcggcaccat
    10981 cggtggctcc atcgccaaca acgacccggc ggccgattat ccggcggcct gtctggcgct
    11041 gggggcgacc atccatacca acaagcgcaa gatcgcagcg gatgagttct tcaccgggct
    11101 gttctccaca gctctggacg agggcgagat catcaccaag gtgtcgttcc cgacgggggg
    11161 caaattcgcc tatgccaagt tctccaaccc ggcctcgcgc tatgcgatgg tcggcgtggc
    11221 ggtgtccaag cgtggcggcg aggtgcgcgt ggcggtgacc ggtgccggtg cggatggcgt
    11281 gttccgctgg gcggaggcgg agaaggcgct ggcggcccgg ttctcggcca aggcgctgga
    11341 gggcaagagc catccggcca aggggctcaa tagcgacatc catgcctcgg ccgagtatcg
    11401 cgcccatctg atcggcgtga tggcgcggcg ggcggtggcg gctgccaccg gaaaatagcc
    11461 acactagagc attttcaagc gaagtggatt ccggttcgcg ttaagaaaat gcgaaaaaac
    11521 aaaaagatag agcactttct gtgaattgga attcactgaa agtgctctaa acatttcgct
    11581 cacaaatagg ggcgttgcgg cccctttttc ttgaggctgg cagatatatt gccggtcaat
    11641 caaccgctca ttaaccatga acgcaggccc ggtcagggtt tcccaaagct taagcgcttt
    11701 gcaagacttt gacgcgacag tcgcaacagg gacggcctcg atgcagggcg agcaacaggc
    11761 cgattgttca gtttcgcgtg cggggacccg gtccggcgca aggttgggtg agtatcatgt
    11821 tgcacaagtc gatgattccg gtcgccgttc tcgctgcctg cctgatgggc gcctccggac
    11881 aagccatggc caagagcggc aagaacaagg cgctgctgac cggcggcgcg gtggggctcg
    11941 ccgccggagc ggtgggcggc tacatgctcg gcaagtcgca gagcgcgccg gcagcgcaag
    12001 cggtggatcg cgagccgacc ggttcgacga cctatcatcg cgcttcggca cgcgagacct
    12061 gttcggtgcg aagtgtcgaa ctgttcgacc gcaagggcaa tttcgtcaaa tccgagcgca
    12121 tgcgcgtctg caattgatgg cggacgctgc ggatcgcgca ggcttgaacc agacggaggg
    12181 ggcgcaaggc ccccttttcg ctgtcagggc aggcgcccgg ccgcccgttc gcgggcgcgg
    12241 acccgttcgc ggtgcagaag gtagagcccg gaggcgatga cgacggttcc gccaaccaag
    12301 gtccagcggt ccggaacatc accgaacacc agatagccgg ccagggtcat ccagatgatc
    12361 tgcgtataga gaaacggcgc cagcaccggc gcctcggtgc ggcgctgggc ctggatcagc
    12421 atccagtggc cgagcgcgcc gccaaagccg agcatggcga tcgccagcca ttccagcgcc
    12481 cctgtcggcc aggaccagag catcaatgcc ggcggcatca gcaccagcga accgaacagc
    12541 gacgtataga aaatcgtggt ctccggactg tcggtggcct ggaggcggcg ggtcatcagc
    12601 aggatgatgg aatagctcgt catcgacaac agcgacagaa tcgccgccgg gtgaaacacg
    12661 ccggaaccgg ggcgtgtcat gatcaggacg ccgacaaagc cgacgatgat cgccgcaaga
    12721 cgccgtggtc cggcccattc ccccaaaatg gggccagcca gcagcgagac catcagcggc
    12781 gccatgaagg cgatcgacac cgtctgggcc agttgcaggt attgcagagc gatgaaattc
    12841 agcgccgtcg acgccagcag cagaaagcca cgcagcgcct ggagaaccgg cttctcgctg
    12901 tgcagcaatc cgggaacgct ccacggattg aggaacggca gggtccacag aaaggccacg
    12961 acataccgca gccagacggt ttccaggaca ccgatgcgcg ggttgagcca cttggcggag
    13021 gcatcgatga tggtgaacat caatacagcg cccatcacca gcacaatcgc gaccatgcgc
    13081 tggcgggtgg tctcgggcgg ggcgtcaggg tgcggcgaat cggtcacggg ccacggctct
    13141 cgccgtcatc attatagacc taagaattgt caggctagag tatgctgcga ttaagtggaa
    13201 gccgtccgcc ggctcaggga tcttcgcgat agagccagtc gaaacggtcg gccagcccct
    13261 tctcgcccat caggcccatg gcaatgtcgc gcgccgtggc aagcgggcct gaggcgtgat
    13321 agatgcgccg ctgacggtgc gacagcttct gaacccgcgc ggtgcgggga atgcggcggc
    13381 gttcatagtt gcgcagcgcg gccgcaacgt ccggcgtgtc ggccaaagct gcggcaaggg
    13441 cggcggcgtc ctcgattgcc tgcgcggcgc cctgagccag gaacggcagc atcggatgcg
    13501 ccgcatcgcc cagcagggtg atgatgccgc gcgaccattt cggctctggc gggcggtcga
    13561 acagcgacca gcgcagccag tgctcggcgc tgccaatcag cgtccgcagg tccttgtgcc
    13621 aatcgatgaa gcgacgattg atccaggaaa tatcgcccga ttccgaccag ccgcgctcgc
    13681 gccagccatc atcgacaacc gcgacgatgt tgatggtgcg gccggattga accggataat
    13741 ggacgacatg ggcgccgggc ccaagccaca ggttggtatt gcgcgcccgc atcgcctcgg
    13801 gcacggcttc ggccggaacc accgtacgcc aggcaaccga tccggaaaag ttggtggggc
    13861 cggtcaggcc gacatggctg cgggcttttg accagacgcc atcggcgccg acgatcagat
    13921 cggcgtcaat ttcggtgacc tcgccatact tggaaaagct ggccatcgtc ctgccggtgc
    13981 gctcgctgat atcctccagc cgctggccga gcatgatggt gacgcccggt gtggccgaag
    14041 ccgctgccag cagcgtcatc tgcagatcgc cgcggtgaat gaccaggaac ggcgcattcc
    14101 agcgtcgcac cgcctcaccg gtatggcgca tgccattgcg ccggcgcccg tcgaggccgg
    14161 tgcggacgat cagctgttcg gtcgcaacag cggtttcggc caaggcagcc tcaagcccga
    14221 gatcgtagag aatgcgggtg gcattgggac caagctgcac gccggcgcca accggctcaa
    14281 gcttgtgggc ctgttcgaaa acgtcgaccg tccatccggc acgggcgagg gtgagcgctg
    14341 ccgtcaaccc gccgatgccg gccccggcga tgaccgcatg gcgttggctg gccatggctg
    14401 catctcctca agccgcatcc gtccacaagg agggcggctg cgactgggtg ccgtgcagcg
    14461 ccttggaata acggaacagc gtcgagcaat aagggcagat gatttcgttg tcggcgccca
    14521 tgtcgagaaa gacatgcgga tggtcgaacg gcggcttggc gccgatgcac atgaattccc
    14581 tggcggcgat ctcaatgacg gcaacgccgg agtcgttgtg aaaatgcggg atcgcgtgat
    14641 gagccatggc gcttcagtcc gggtgtgcga aaaggtgcgt tcacctctag ccattccgga
    14701 cgcggattcc tagaggcctt gtctgcaggc gggactattt gccaagtgtc ggaatggcga
    14761 caggcttgag ccggacagga gtttcgtgtc aggcaacgat cctgcaagag ccggagggga
    14821 aatgcacagg ttcgttcata agggcgtagg actcaactat atcgacgtgc cgccccatga
    14881 aggcagcggg ccggcggttc tgctgatcca cggctttgcc tccaaccatt cggtcaactg
    14941 ggtcgcgacg caatgggtcc gctcgctgac ccatgccgga taccgggtga tcgccctcga
    15001 caatcgcggc catggcctgt ccgacaaact ctacgatccg gccgagtaca ccacattcct
    15061 catggcggac gatgcggcag cgctgcttgg ccatctcggt atcgaagggg ccggcgtcat
    15121 cggctattcc atgggggcgc gcatcgccgc ttttctcacc cgcgcccatc cacggctggt
    15181 gcgcgtgctg gcgatgggcg ggctcggata ccggctggtc gatggcatcg gcctgccgca
    15241 aagcatcgcc gaggcgctgg aggctcccgg cctcaacgcc atcgaggacc cgatgggccg
    15301 gatgttccgt gctttcgccg agatgaccgg ctccgatctc agggcgctgg cggcctgcat
    15361 ccggggctcg cgccagacca tgccgctcga acatgtccgc atgatcgcca ccccgaccat
    15421 cgtggccgtc ggcaccaacg acccgatcgc cggcgacgcg cggcggctga ccgacgtgat
    15481 gcagaacgct gtccccttcg acatcgaagg gcgcgatcac aatgtcgcgg taggggacat
    15541 cacccatcgc aaggcggtca ttgccttctt cgacaatcat ctcaagaagg agccctgacc
    15601 cgttgccggt caatggcttg gcgagacttg ctgtgattct gattccgcct tcttcaggtg
    15661 atgattccgg tcgttgaagt atcgaatcat accagtggtc cagacaattg accgtcaggg
    15721 gccattgtca gggccgctgg tcaggagcga atgcgacgcc gagcacggcc gaaggcccgg
    15781 ttgcgaggga cggcccggga aatgaccggc aggtcaattt gtaagggcca ctggtatcag
    15841 gccggctctc cggcgggtgg ctggcatgcg gacggacagg ggccgcgtcc gcatcctgag
    15901 cgccgggctg gcaggagcgc gcacgaggca ttgtcaacat ttctgtgata ggtctcgttt
    15961 ccggtcggtt tggcgcctat gtcgggttct gcgatcggcg agcagcgagg tggtgcatgt
    16021 ctgccaatgt gcatgggctg aaggccaatg tggcgccggt cgatccggta tggaggcggg
    16081 tgcgtcttga ggccgaggcg gttctgaagg ccgagccgga aatggccggc ctggtgctgt
    16141 ccaatatcct gcatcagccc tcgctggaag cagcaatggc gcaacgtgtg tcatcgcggc
    16201 tcgatcatga cgacctgtcg ggcgcgttga ttcgccaggc gttcgaggaa atgttcgcgg
    16261 cccagccgga cctcggccag tggctgcgca tggacatcat ggccgtcctc gatcgtgatc
    16321 cggcctgcga ccggctgatc gagccgatcc tttatttcaa gggctttcag gcgttgcaga
    16381 cgcatcggct gacgcattgg ctgcgcagca aggggcgggg cgatttcgca ctctatctgc
    16441 agagccgctc gtcgaacgtg ttccaggtcg acatcaaccc ggcggcgcgg atcggcaagg
    16501 gcgtgttctt cgatcacgct accggactgg tcgttggcga gacggcggtg atcgaggaca
    16561 atgtgtcgat cctgcagggc gtcacgctgg gcggaaccgg aaaggaacat ggcgaccgcc
    16621 atcccaagat ccgccatggc gtgctgatcg gggcaggggc caagatcctg ggcaatatcg
    16681 aggtcggcca ttgcgcgcgc gtcgccgccg ggtcggtggt gctgcaggat gtgccgcaca
    16741 acaagacagt ggccggagtg ccggcccgca tcgtcggcga ttcgggttgt gccgaaccgt
    16801 cacggctgat ggatcagatc ctgcacggtg atcccggcat ctgaggatgg cttgaacggc
    16861 gccgcgcaac gtggcaagga cgggcctgcc gatcaggcgc caacacgggg agacagcctt
    16921 ggagaagccg gacatccagc gcgtggaaag ctatctgcgc cgcaccttca acaatgccaa
    16981 catccggctg atggcccgcg ccagaaagaa ggattcggcc gaggtgttca tcggcgagga
    17041 attcatcggt cttgtccatg aggacaagga ggacggggac ctgtcctaca atttcaccat
    17101 ggccattctg gataccgatc tcgaataggc tgtttcacgg ccgggctggc ggctgtgtcc
    17161 gcagcacgct tgccagagcc cggatggcag aggtcagccg ctgccagtcc tgaatgaggg
    17221 cgggcgcaaa gcggacgtgc agttccaggc cggcctgacg ggtttgccac aggcattgtt
    17281 cggccggctg cggaatggcc gcccctcgcg gacaacgggc cgaaaactgg cgctcgtccg
    17341 gtgttgccat gaacagatcc tcgccctcgt agggagaccc cctggaaaag cggcgctgca
    17401 ccagcccggc cgggccggtc accgcgtccg cctcgagaaa gcgcgcataa agctccactg
    17461 gccgttcggc agggtcgata tcgtcgcgcg acttggccag ggcgctcgaa tcctcaagcg
    17521 acagaaacac cagcgctggt gcggtcttgc cgccgaggtt gccggccgca ccgctcagat
    17581 ccggccagtt cagcaccaga tcgagcctgt tgacggcgcc gccatcgcga agatccaggt
    17641 tgcggaccat ggcgcggggc acggtcagcc ggtgcgatcc gacgtgaaac accgcatggc
    17701 ccgacggcgg gcgggtctcg acatagatgc gccgcgccag cattccgccg atggcggcaa
    17761 ggccggcaat gcagctgatg acgagcagtc gcaccatgca cccatctttc cagaaacggc
    17821 ggcgacccta gagcatgctg cgatcaagtg gaagccggtt gatcgccgaa gcatgctcca
    17881 accacctgaa aacgatcacg ttatctctcg ctttgatggt tccatcaaag cgagagcgtg
    17941 atctgtgcga ttcgcgctac tggcacgaat tccgcaacgg gcggcctgaa aggcagggag
    18001 cgccgcaatc tgcgccaatc cggcacaggg cgtatcgcaa aaggaaacaa tgggctggga
    18061 tgcgctgtcg gctctggcga cgatgctcgc ggggctggtg tttgccggcg gattgatcga
    18121 ggcggtggcc gggtttaccc gtcagcgcat cggctttgat ctggtctggc ggtctgtgag
    18181 gctcggatgg ctggcgctgc cgttcctgat catggccggg ccggcgatcg tgctgcggga
    18241 aaccgccctg cggcgaaccg ggccagggtg gaatggcgcg ctgtttgccg tcggcctggt
    18301 ggtcgcgctg gcctgggcac tcgcctcggg tcatgtgctg ctgtcaatca tccgggcgag
    18361 cgagccgctg ctggtcacgg gccaggtccg gctgctcaac tgagtgcgga ctgcaaggtc
    18421 aaacgggctg tcgtaagggc tcttgggctg agcatctcgc atgctcagaa aggggcggag
    18481 ccggcccccc ggggaacggg ggccggctcc tttttcagat ccggtctggg gacggggagg
    18541 gtggggaagg accgaacctg aaattccgat cagcgcattc gcggccggcg cgacacggcg
    18601 accgtcgccg tatcgatgcc gccaagcgag acccagcgat tgggtgtctc ctgcgactgg
    18661 cgcttgatga agcggtagcc tgtctcgtgc caggccagca ccttgctcgc ctgattgtcg
    18721 agaatgaaat cgccatggtc ggtcttgacc gtcaggacgg catggccgtc gcccttgttg
    18781 tcacgcacga ccgtgatgag cagcgcctga cgcggaaagc cggcccgcat cagacgcttg
    18841 cgcttttcca gaacgaaatc ctcgcaatcg cccttgccat ccgtcggata ggaccacaat
    18901 tcgatcacgc catggttttc catgtcggtc accggagtga tctcgcggtt gacggcagcg
    18961 ttgatgcgca caatctcgcg ccagctggtt tcgtcgagcg gaatgatctc ggcgggaagc
    19021 gcctcgacca tgcattcgcg gccgctgcgc tcgcagaatt ccacccagcc gatcgggggc
    19081 gtcgtctcgg cgccggccgg cggtgcatgc atcgccattt gctgggccgt ggcaagctgg
    19141 gcgccgacgc agaacattat cccgccgatc agtccgaacc tggctatctt ggttgcatgc
    19201 ttcccgaaca tttgtctctc cccaaccgtc aggtcgagga ttggctgcat gctcttgaat
    19261 tgacttggat ttttccggga agactcgagc gttccggttc atgctttgct taccatgggc
    19321 agcctccggt gctaggcagc ggtgcgtcct gcctgctcca gaaatccata agctgttgta
    19381 ataaaagcag aaaatggctt ttgtgaagca attgattcaa cttgtcgcaa ttggcctaac
    19441 cccggctttg ctgtgcccct tagggtcctg tcagggttga tgcggattca tcagtggggg
    19501 ccgtgcggga tcgtgcggca gcgcacggca gaaagcgggc cgggaccatg aaaaagcccc
    19561 gccgaagcgg ggttcatgtc gatcgagcca aaggttgtgg ggagaaggcc tgtccgggga
    19621 gcaagcgctc agtgtggtga tcgcttgacg cgcgcactgg ccgcgtccga gggactgtga
    19681 aggcggcggg ccagagcact ttcagcgaat tccacttcgc ttgaaatgcc ctctcttttg
    19741 gctttttgca ttttcttagc gcgaaccgga ggccacttcg cttgaaaatg ctcaggtgag
    19801 gaggtcagac ctcgaacgct ccgggggccg gcgggcggct gaattccacg gcaataccgc
    19861 gttcaaacac gcgcaccacc ttggatgggg tgttgttgag catcacgaga tcgccgcggg
    19921 tgaagctgag atcggtcgtc agcgcggcgc cggacaacga aacgtcgatg aggcggacca
    19981 tgacttccgc gccgccggcg gtgaccaggc ggccgtgcgg gttgcgcggc acatagcgtt
    20041 cgtggcggcg gtcctcgggc aggccgagtt catggcggtt ggcgagccag gtcagctgcg
    20101 cggcgagctt gtcttgccgg cgcagggtgg cgttgatggt catggcaaaa ccgtcctcgg
    20161 tgaggcgggc gatctgcccc tcgatccggc cgacggtttc aagataggcg atcacgcggt
    20221 cattgacctt gccatgggct ctggtgcgca gccgtgcgcc accgggcgac atgtcgatcg
    20281 tcacgcattc atgttcgctg tgatcgggca gcatgaaccg gccatgcagg atcacctcca
    20341 cgcgctggaa gcggcgttgc tcgactggat tgatggctag ctttgaaccg gacagcagca
    20401 tgcgagattt cccgacagaa tcaccacgag cgacgtgatt cctgatcgga acaatagtcc
    20461 ccaagcgtta aagaagccgt taagcctgtt gcgcagttct cgtcaggggt gctgcccgcc
    20521 ttccagaacg gtcaggtggc cacgccgttc cgccattccc ctcaggtgaa tgggcagttc
    20581 gggctccggc gggtggagga agcgggtctc gctgtcgtcg atcaccctga agctgatgat
    20641 gtcgatgccc aaacaggcaa cgcggggtgg cggcagctcg gcgccgaagg tcagacagcc
    20701 cagcagccgc tcgcccggtt ggctcctgtg cagcagcggc aacaaaagca actcggcggc
    20761 ggccggtgcc tcgttcatca gcagtaccga ggctccggcg atgacagggt tgaggtcggt
    20821 ggcaacgatg gcaaggatgt cacgcagatc atccactgct tccggggcca tgagggcctg
    20881 gaggtcggtg ccgcgcaact cggtatcgaa caaggcgcac aggcgcgagc cggcgatcct
    20941 gatccggaat gcgccgtcgc cggttccttc cagcacgaac atgtggggca gcaggcggcc
    21001 aagatcggcg gggttgatat ccgcccgttc gggggcgggg cggggaccgc gcaggcgccg
    21061 ccaataggca aacaggtgct tggatacggg gtgtcgcatg gtgccgcctc ggtcgtgttt
    21121 tctttcggaa cagcagcgag agacttgtgc cggaatgctc cggggaagtc aggcgcgtgc
    21181 tgtcgtccgc gacgccgtgc agagggcatg ccaagggcgt taacgtttgg gcgacggtga
    21241 atttaacctt aagcccagcg gccgtttgca tttgcgaaag atgtatgaga ccttggtgac
    21301 gctccgattg aattgacccc cgcgccgctt cgctcccggc gcgggggttt gcttttcagc
    21361 ctgtgcagtt ccttctcccg gcgagcaaga ttccggttgc cagatggcgg gcgggcggca
    21421 attctggcca tggccgcatc aatgcgcgga cacatcggga gggacagcat gatcgttgcg
    21481 catattctca accagaaggg tcgcgaggtc gccaccgtcg agccgggcca gcctgtttcg
    21541 gatgtggtct cggtgcttgg cacccgctcc tacggggcac ttgttgtgtc tggcagtgac
    21601 cgcaggatcc agggcatcat ttccgagcgc gacgtggtca agctgctggc ccggtccgga
    21661 gccggcgcgc tgagcgaacc ggtgtcaaaa cacatgaccg ccaaggtcgt gacctgtgct
    21721 gccgacgata cgatcgatca ggtgatgcag gcgatgacca agggccgctt ccgccatatg
    21781 ccggtcgagc atgacggcaa gctcaatggc atcatctcga tcggcgacgt ggtcaaggcc
    21841 cgtctcgaac aactcgccaa cgagagtgag gcgctgaggg cctatatcaa cggctgaccg
    21901 gccatcaggt tagagcatgc tgcgagaagt gtgggcaatt tcgccaaaag catgctctaa
    21961 taccggtggt cctcaggatt gacctgttcg cctcgggcga aacaagtcaa tttcggacct
    22021 gttcctcgca tgggcccggc gtcgcattcg ctcctaacca acggccatcg gtcaaacccc
    22081 atgaccgttg gtataacatc ttgttttatc gcatgttctt gacgcgaacc gggttccgct
    22141 tcgcttgaaa acgctttata ccagcggccc tgacaattgg ccattagggg caattggcag
    22201 gcggctggtt aggagcgaat gcgacgccga gcagggccga aggcccggtt gtgagggcca
    22261 ctggtattat tgccgggcgg cgagcgcgtc gaaaacctcg cgcagatccg gcaacatgcg
    22321 ttcggccgct tcggcgccga gcgcgattgc ctcatcggcg cgatgaaact cgaacaggcc
    22381 gatccgcgcg agcttgggcg cgatcatcac atcgggcggg tcgccagcaa ggcgcgatcg
    22441 ggcgatgcgg tcctgggtga tgttgatggt ttcgatcatc accgaggcga tggacggcgt
    22501 gccggacggc gtcgagggcc ggccgttgaa gtagtaggag acggcattcg ccgcgtcctt
    22561 gaccggacgg aacagtccgg agcgaccggt cgcatccggg gtttcggccg gtggcggttc
    22621 agcgacgcgg tcggaaatca cggtcgagcg gccgaaatgc tcattattca gattgacggc
    22681 gatcaccatg gtcgcgccga gcgcgcgggc agtggtcaca ggcaccggat tgaccaatgc
    22741 gccgtcgagc agccacttgt cgttgacctt caccggctcg aacacgccgg ggatggcata
    22801 ggaagcgcgc acggcagtgc cgagatggcc gcgcttgagc cagatctcgt ggccggtgcc
    22861 atattcggtg gcgacggcgg caaagcgcgt cggcagattc tcgatggcgc ggtcgccaag
    22921 ggcctcgtcc agcatctcgc gcaggcgtcg cccaccgaca aggccggagc cggagagcga
    22981 gaaatccatc agattgaaaa tgccgcgctt ggtcaggcga cgggcaaaga gctcaagctg
    23041 gtcgaggtat ccggtcgccc agcagccgcc aaccaccgcg ccgatcgagg ttccggtgac
    23101 gatcttcggg cgaatgcctt cgcgttcgag aaccctgaga atgccgatat gcgcccatcc
    23161 gcgggcgacg ccgccgccaa gggcaaggcc gatgaccggc cccttcggct ggggcttcgg
    23221 ctccggcaag ggagtggaca cgacttccgc gctccgtcga aactgcaacg ccgacagcac
    23281 catgcggttc cccccaaggc gggcgggatc ggcgtcgtga tccgcacctg tggaccaggc
    23341 aaccgtccgg ttatgcgatg acccgaggac cagcctgcgc gcaacaggtg actgaaagcc
    23401 taaccgccgg acgtttcggt gaaggacagc gttaatgttc cgtcaccggc gcgggacagc
    23461 gtgcggtaga agcagctctt gcgcccggta tggcaggcag ggccggtctg gcgtacggtg
    23521 accagcaggg tgtcctgatc gcaatcggtg gcgatggcga tcaccgtctg gctgttgccc
    23581 gacgtctcgc ccttgcgcca gaaggccttg cgcgagcgcg accaatacca ggcctcgcct
    23641 gtctcgatgg tgcgccgcag ggcctcggcg ttcatgtagg caagcatcag cacctggccg
    23701 ctgccggcct cctgcgcgat caccggaatg aagccatcgg catcgaattt cggcgtgagc
    23761 tgcgtgcctt gctccagttc gaccttgtca ccgggagggg caaaaggggt tttcggggtc
    23821 cacatgccgc cttgtctctg ccggacggcc ggacctgtca aggcggacct cgcaaacctc
    23881 cgcaaaagcg attacatctg agacgatcag ggacgcgcaa tgctcaacga cgtctacaac
    23941 cagcggattc tcgaactggc cggcaatatt ccgcggcttg gccggctcgc cgcgccgatg
    24001 gccagcgcga ccgcccattc caggctctgc ggttcgaccg tcacggtcga tctggcaatc
    24061 agggatggca aggtcagcga ctttgcccat gacgtgaaag cctgcgcgct gggacaggcg
    24121 tcctcgtcga tcatggcgcg caatatcgtc ggctcgaccc cggccgaact gcggagcgtg
    24181 cgcgagaccg taaggcgcat gctgaaggaa aacggagcgc cgccggccga cggcaaatgg
    24241 accgatctgc gtgtgctcga accggtgcgc gactacaagg cccgccatgc ctccaccatg
    24301 ctgacgttcg atgccgtggt cgaggcgctc gacgcgatcg aggcgggcgg caagggccgg
    24361 taggatgccg gcctttcgct ggtcactcca ctggcttgtc gcctttgcgc aggccatcgc
    24421 aggcgttaag gaacgcccgt ccggcactgg caaaggatgc ggtcgggatg acaatctcgg
    24481 agcggcggtt ttcgtcctcg tcgagcatca cgcgcaggcc atcggccggg cgggcgagca
    24541 gattgcgcaa gggatcgtcg acaatgaccc ccgacttgcc gatccagtgc ccggcattgg
    24601 tcgccgccag gcgcggaaag cgggcgggca gggcgatgat gtccttgccc gatgccgcaa
    24661 gggtggccga tgtctcgccg gaagccagaa cggcacggga gagagtgacc ggggcggctt
    24721 ctgtcggcgc cacaaccggg ctttgctcga agcgggcgcg caggaagccg gtgtcaggat
    24781 ggcaggacag ttcaagcaca tagcgcagat ccggcttgcc ggggcgccgg gccagaccgc
    24841 cggttgacag tgacaccgtg cgatccgccg tcgccagccc ccaggctccc tcgccggaaa
    24901 acagtggaac gccggtcacc accgagccga ccttgagttc gacgatctcg cgcgggctga
    24961 ggcggcggat cgaattgcgg ggcggggacg aggcggcaag ctccagcaac ttgaggtcaa
    25021 cgcccatttt ctggatgtag gtgccgaggc gggcggaaat gcgctgggcg cgctcgattt
    25081 cctcaaagcc gtagcggcgg ccgaacaggt cctccatctt gtccgatggc cagaacatgt
    25141 gcacgccata agaagcgccg atcggcacga agcggtgccg gccgccaagg aaagcatagg
    25201 cgcaggagga aaagcaaagg ccgagattgg gcacgagctg gtggcgcggc aagcgcgccg
    25261 gaccggccgc tccctcggca tcgccattgt cgactgcggt tgaccggccg atggccacgc
    25321 gcgccctgac ggtgcgcagc cgctcgccca gttcaaggcc gccgagaatg ctgccgccct
    25381 tggaatcgaa atagatcgtc agcgtctcgt cgagcgggtt gtctttcagg aaggcatcga
    25441 agcgcctggg cgtgtcgttg gtgatggcgc cgctggcggt gacccagcgg atctgccgtt
    25501 cgcgcggggc gtcaggattg ctggagacca gacggaaggt catcggctcg ggcgatgtcg
    25561 ccggcgtcgc aacaggcttg cgaccctgcg ccgccacggg aagcgccaag agtccaaaca
    25621 caaccggcag gaggagccaa tgccttcgca tcagcccatc ctgccggcaa accatgaaca
    25681 aaatcgggcg aaagacggtt accagaaacc cttcatcggg aaggaatagg acagggtcag
    25741 gcccaccgta tactggttgg tcgaaccaag ccgcgcggtg atcgggctgt tggccgcaga
    25801 gcggaccagc cggtcatagc cgccgtgcag cgtcgccgac caggtgtcgc tgaacttgta
    25861 ggtggccgag gcatagatgc caaccgactt catcccggat ttggcgcggt atttatagac
    25921 acgcgggttc agcgccgcat cgcgggcggt cacaccgaaa tggttgcgca tgtaggagcc
    25981 gtcgccgaag gccaggcgcg ggccgagagc gaccgtccag gaattgaagc tgttgaccag
    26041 atcgaggccc agattggcgg caaagccgtc cttttcgcgg aagccgcggc ggatttctgc
    26101 gcgggcgcgc aggttcgggg tgatccaggc gtcggcgaac agacccgatt caagggtcca
    26161 gcgtgcatcg tggatgccgc gcaggtcctt gtccgatcca tcatagcgac cgccgacata
    26221 gcggaacacg gggccggccg tgaacacctt gttgtccaga aggccaaagc cgatgccatc
    26281 atcgggggag gagaatttcg gcgcctcgcc caccttgcgg aacgagaccg tggggtaggc
    26341 aatcgcgctc atcacgccgg cgccctgcca tttcggcgag gcgaaagcat tggccttgac
    26401 ggtgacgatg tagccgtcaa tcctcgctgg ctcgaacgcg acccgggtgg tatcggctgc
    26461 cgccgccgga cggtggccag caaagatggc gagaaccgcc aggaaacacg caacccaacg
    26521 aagcatgaca caaccttacc cttgtccccc acagtcgatt taccctgatt gggttaacgc
    26581 tccgccaaga tggccggtgc agcgtggaaa aatcacatga aggatgaagg gcctggacgg
    26641 ctcagccggc tgatggcgct gccgctgatc gggctggtca ggatttaccg ctacagcctg
    26701 tcatcactga tgggacggac ctgccggtat ctgccgacct gttcggaata taccgaggag
    26761 gcgctgcggc ggcatggtgg ctgggccgga ggctggatcg gcctggcgcg catctgcc
//
LOCUS       DAOMXG010000010        26775 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_1143_length_26775_cov_11.180614, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000010 DAOMXG010000000
VERSION     DAOMXG010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 26775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26775
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1143_length_26775_cov_11.180614"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..152
                     /locus_tag="PLQ11_01435"
     CDS             <1..152
                     /locus_tag="PLQ11_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1674 domain-containing protein"
                     /protein_id="HRK23595.1"
                     /translation="RALAEAAARRRAQEEREAVLKRQREINGRGGLDPVRYEDWEVKG
                     IASDF"
     gene            256..810
                     /locus_tag="PLQ11_01440"
     CDS             256..810
                     /locus_tag="PLQ11_01440"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HRK23596.1"
                     /translation="MRTSSLFALVLLLALCGSALSRRPAAETAMVAASRGVNAPVAPG
                     SLPATRPDRSRPLLQGPLAAELLSVIDGDTIEVLVQTFIDQTTRVRVRLRGIDAPELS
                     TACAEEHDLAQAARNALAGLLAGGRLILTEIGRDKYAGRVVARVLSVNGEDVGARLLA
                     EGYALAYAGGRRQPWCDTSLTALR"
     gene            complement(794..1264)
                     /locus_tag="PLQ11_01445"
     CDS             complement(794..1264)
                     /locus_tag="PLQ11_01445"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003598583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRK23597.1"
                     /translation="MPAIGVSFSAEARERVPLPGAYSAGSIIVSTGQRRLYLVLGDGS
                     ALRYPVAVGRPGKQWFGQRVVDGKYVRPAWSPPDDVRRDNPHLPNVIPGGVPSNPMGE
                     RAITLSPGQYAIHGTNVPGSIGRPASYGCIRMFNHDIVELFERVSLGTTVTVVR"
     gene            1501..2880
                     /gene="hemN"
                     /locus_tag="PLQ11_01450"
     CDS             1501..2880
                     /gene="hemN"
                     /locus_tag="PLQ11_01450"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019566222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HRK23598.1"
                     /translation="MSAPETLALSAETIRALATPVPRYTSYPTAPHFNAGVSAADYAR
                     WLSELPAQSRLSLYMHIPFCDTLCWFCGCSTKITQKYAPVSSYLDLLVREIGLIGTLV
                     PSDARVAHIHWGGGSPTMLTPPDIARLAAATRAAFKLDIDCEFAIEVDPRGLDVAHID
                     ALAKAGLTRVSIGVQDFDRQVQQAINRVQSFEETRAAVEGFRARGITSLNIDALYGLP
                     HQTTERMIETLRQVMLLRPDRIALFGYAHVPWMKRHQAMIDEAALPDVVERYQQAETA
                     AAYLVAHGYQRIGFDHFALATDSMAIAARDGTLKRNFQGYTVDPADALIGLGATAIGA
                     LPQGYVQNDTATGRYMTAIGKGMVATEKGLALSAEDRLRRAVIERLMCSLTFSAAEVR
                     ALAPSADLARTLIEEARQVVAEDEYGFVAATGDGFVITEAGRPFMRSIAARFDAYLPR
                     RTARHSVAV"
     gene            complement(2881..3585)
                     /locus_tag="PLQ11_01455"
     CDS             complement(2881..3585)
                     /locus_tag="PLQ11_01455"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRK23599.1"
                     /translation="MAAILFDKDGTFADFSATWGPAIDSVMRSLAKGDEAAYQRLATF
                     NRFEPSTGRLYPDSPFIAQASADFAAGWARALGVSFDTDFHRMMDRLLDAAALAHVTP
                     IGDPGAVFDRLRGRGLRLGVITNDTEAGARAQCDRLELTARLDAIIGYDSGFGRKPGP
                     GQILAFAERFGLVPSRMAMVGDTTHDLAAARAAGCLAIGVLSGFASREELAPHADLVL
                     DGIEALPAWLNNADLP"
     gene            complement(3607..4095)
                     /locus_tag="PLQ11_01460"
     CDS             complement(3607..4095)
                     /locus_tag="PLQ11_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HRK23600.1"
                     /translation="MSRDGEAAGRVALYRTVKQFGHSEGLSCCFRQWRATHSHCTFLH
                     GYALAFTFHFVTDRLDANNWCYDFGGMKEVRAWLHSMFDHTVLVAEDDPDLETFQDLA
                     RRKIVDLRVLPRVGCEAVAELAAAHVAAFIDKKTRGRVWLERVDVAEHDGNSASYAPP
                     SG"
     gene            complement(4092..4760)
                     /locus_tag="PLQ11_01465"
     CDS             complement(4092..4760)
                     /locus_tag="PLQ11_01465"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF013398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HRK23601.1"
                     /translation="MKSNILRMTAARLCNEAVDGYARPAEREALKAAAAQKIEELFDI
                     LRIDHRNDHNTRETPQRVAKMLVEELLAGRYETPPKITEFENVRCFDDLIVTGPIEVR
                     STCAHHMMPIYGHAFIGVLPSPDGTIIGLSKYDRIVQYFAARLQIQEELVKQIQDFVV
                     EKTHPRGLAVRISAVHMCKTHRGVRASHASRMVNTSYYGELDRNAALRAEFLQECATL
                     GPRG"
     gene            5010..7481
                     /locus_tag="PLQ11_01470"
     CDS             5010..7481
                     /locus_tag="PLQ11_01470"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRK23602.1"
                     /translation="MKRVRIVRLVSAIVALLLLAGAGYQGFQIRAMKQDSAASSHIAL
                     ARQSADASAELASLIGKVGELGVAATAYRDSSGLAGVRASMASLQQHQKELAAPIHKA
                     FIAADSTHARVPADLAAMLTQLDRRLGNLTERRFVPEVLELLSAMERPLLGLAHAAAE
                     HSSRALEAERSLLTGMNHRLMAWLAGLALAMMVFLGALLSELNVLYRNNSQLNVVAGS
                     LEKTKGDLETANKSIQNAYAELNLHNQILKVHDKEMRTQNLRFDAALNNMTQGLCMTG
                     ADGKLIVCNEQFRQLFSIDKETARPGTDMTAILVRMGVALKLGETGTAEWMRTHDTLV
                     AAAKHEAFTRDCPDGRSFSIHHQPMTDGGWVETYEDITERRKAEEKIFHMAHHDALTG
                     LPNRVYFMQHLEEALGRVRRGEAACAVLCLDLDRFKAVNDTLGHPVGDLLLKEVAARL
                     RAAVNPSDVVARFGGDEFAILKNSDVEPEALAGLGEKLVKSISAPFHLDGNEVTVGTS
                     VGIGIGFQDGASTEQLLKNADLALYHSKSEGRGAYHFFEAHMDAKAQARRLVETDLKR
                     AIPAGELDLFYQPLVDVMTRRIVGFESLIRWRHAEKGMISPAEFIPIAEEVGLIIQIG
                     EWALRRACATALLWPEDIKVSVNLSPVQFRDRNLVQVVKNVLAETGLAPHRLDLEITE
                     TALVQDIDFTVKVLLALREMGVQVSLDDFGTGYSSLSYLRSFPFDKIKIDQSFVRDLA
                     TRPDCLAIVKSVTTLGENLGMKTLAEGVEEEEHLTILRELGCQEAQGYLFSRPVPNPE
                     ALKLIETFNGVTIAAPAAAKAAVAA"
     gene            7635..9434
                     /locus_tag="PLQ11_01475"
     CDS             7635..9434
                     /locus_tag="PLQ11_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015441 - ABC-type beta-glucan transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015775 - beta-glucan transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan ABC transporter ATP-binding protein/
                     permease"
                     /protein_id="HRK23603.1"
                     /translation="MSLFRLYARVIAQLGNELRMGMLLMFANLVLAIAQFAEPYLFGR
                     IINLLTGTSGGTKPTLDALMPLIIAWLVFGLFTIGGGVLVALHADRLSHRRRLGVMAG
                     FFEHVLALPLAFHSQTHSGRVLKVMLEGSNGMAWLWLGFFRDHCAALISLFVLLPFTL
                     FVNWRLGLLLCVLVVVFAVLTAIVLRKTETKQGAVERYHTDLAEHASDAFGNIPVIQS
                     FTRVQAELHHLRDIIDRLLTAQIPVLSWWALASVATRAASTLTVTAIFLLGTWLHYQD
                     LASIGDIVTFMGYAGLLIGKLEAAVGFVNSLFMQAPKMEEFFEVLDTTPSVHDKPHAI
                     ELSRPEGAVRFESVSFSYDGKRAAIKDVEFSVEPGQTIALVGATGSGKSTTLGLLHRA
                     FDPQSGRITVDGHDIRDVTLLSLRRNIAVVFQEPMLFARSIRENLQVGRPDATDEEMM
                     EALKRAQALEFIERQDAGLDTVIGERGRSLSGGERQRLSIARALLKDPPILILDEATS
                     ALDATTERKLQAALDEVMKNRTTFVIAHRLATIRNASRIFVFERGEVVENGNFEQLVA
                     LNGRFATLARAQFMAGSDQAAQTPASADPVVYG"
     gene            complement(9425..10561)
                     /gene="aroB"
                     /locus_tag="PLQ11_01480"
     CDS             complement(9425..10561)
                     /gene="aroB"
                     /locus_tag="PLQ11_01480"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006470978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HRK23604.1"
                     /translation="MSAPFFTVPVALAGRSYDIRIGRNLIDTAGDEIARLVPGARAAI
                     ITDQTVAGLHLDRLTRSLASAGIAARTLVLKQGEATKNWTELGHVVEALLADRLERRD
                     IVVAFGGGVIGDLAGFAAAIVRRGMAFVQIPTTLLAQVDSSVGGKTAVNSPHGKNLVG
                     AFHQPILVLADTALLDTLSAREFRAGYAEVVKYGLIDQPAFFDWLEAGHWRGILDGSA
                     DREEAVRISCAAKAAVVARDETETGDRALLNLGHTFGHALEAVTGYNSARLVHGEGVA
                     IGIALAHRFSQRLGLCTGQDALRVSRHLQAVGLPTRLQDVPGGVGSADELMAAIRQDK
                     KVSRGALTFILTRGIGQSFIARTVDPGAVVSFLTDELNERTPQP"
     gene            complement(10561..11166)
                     /locus_tag="PLQ11_01485"
     CDS             complement(10561..11166)
                     /locus_tag="PLQ11_01485"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HRK23605.1"
                     /translation="MSKNEPDAVPVTAAAQGRTIVERLGDRSIVLVGMMGAGKTSIGK
                     RLGQFLDLPFIDADDAIEEAADMSIPDIFARHGEAEFRAGERRVIARLLKQGPQVLAT
                     GGGAFMNEATRQAIREAGLSLWLKADFDVIMRRVRRRSNRPLLKTDDPEATVRKLIAE
                     RYPVYALADITVLSADVPHEEMVDVVVAALANHLAAQPEQS"
     gene            11373..11516
                     /locus_tag="PLQ11_01490"
     CDS             11373..11516
                     /locus_tag="PLQ11_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23606.1"
                     /translation="MLAIVRLLVFFSVLAAIGFGSMIALVQFVEPEQRDYSIVVPPSK
                     FNK"
     gene            11521..12468
                     /locus_tag="PLQ11_01495"
     CDS             11521..12468
                     /locus_tag="PLQ11_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HRK23607.1"
                     /translation="MAPRVSRRTAIDDFLDMMAAERGASINTLAAYRRDLDDYRAWLA
                     ATGHDEASVVTNAIRAYLADLAGRGLKASSAARKLSALRQFHAFLYREGRRSDDPTVI
                     IEGPKRSRPLPKSLGVDDVDRLLAVAAEGCGDASRPLADRIAAARLSALLELVYATGL
                     RVSELVTLPTEAVRSGRDTMMVRGKGRKERLVVLSPAAIAAVQRYRDLTTPKGGPRPK
                     FLFPAEGAAGHLSRQVFARDLKRCAAAAGLDPERISPHVLRHAFASHLLQNGADLRIV
                     QQLLGHADVATTQIYTHVLDERSRSMVRDLHPLTDTDDR"
     gene            12563..13513
                     /locus_tag="PLQ11_01500"
     CDS             12563..13513
                     /locus_tag="PLQ11_01500"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HRK23608.1"
                     /translation="MRYFDFETSVAELENKLAELKALASTGGNRDIGDDVRKLEGRAH
                     DMLKTLYASLTPWQKTLVARHQQRPHCIDYIRELITDFSPLAGDRSFGEDEAIIGGFG
                     RFNGQSICVIGQEKGTDTESRLRHNFGMARPEGYRKAVRLMEMADRFAIPVLCLIDTA
                     GAYPGIDAEERGQAEAIARSTEACLALGVPNVAVIIGEGGSGGAVAIATANRVAMLEH
                     SIYSVISPEGAASILWRDSARAQDAASTMKITAQDLLKFGVIDTIIPEPVGGAHRDPK
                     AAINNAGAVIQTALGELVQRGPEELRTARAEKFLAMGRKL"
     gene            13776..15068
                     /locus_tag="PLQ11_01505"
     CDS             13776..15068
                     /locus_tag="PLQ11_01505"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase family protein"
                     /protein_id="HRK23609.1"
                     /translation="MSEKGTNKHAPVVLRAFKKESELEIWKQNAAGEYILLKTYPMCR
                     WSGQLGPKVREGDRQVPEGFYSITPTSLNPNSSFYLSFNVGYPNAYDRAHGRTGSAIM
                     VHGACSSAGCFSMTDEQIAEIYAITREAFAGGQKAIQLQSYPFRFTTQNLAKFRADPH
                     MPFWKMLKEGSDSFELSKQEVQVSVCEARYAFNRGQRTDGSPVCGPQDGTSPLVQAVA
                     AKARSDEQKVAELAASGVGAVRRIYKDGDQHPAFRTTHFAATDSEGRTIASPRSSASR
                     VAEVSRPDALDHGPVEVAVDSLKGRGQAPAAALARTNLATQAPALPSPPPVVAAPATP
                     AAPSANAFSPTVTGNSSIFRGLLGGSPEPSRPDVVTAVEPDRPQPVRIPLPPRAPQRQ
                     AGQPAAPLEAAPAEIPNQPSASFQTVLKGFNALIPGMR"
     gene            complement(15113..15643)
                     /locus_tag="PLQ11_01510"
     CDS             complement(15113..15643)
                     /locus_tag="PLQ11_01510"
                     /inference="COORDINATES: protein motif:HMM:NF022115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2478 domain-containing protein"
                     /protein_id="HRK23610.1"
                     /translation="MRSSSLAAVLYREGASCDALLADVARQLAGEGYRLAGAVQTNDR
                     YDALCACDMTLSDLNSGRSIRISQRLGQYARGCRLDPGAMAEVVSLVEAGLAGGADLL
                     ILNKFGKSEAAGHGFRPAIAEAVLQGIPVLVGVSAANLPAWMEFTGGEADVLEPDAAA
                     ILAWIGDPPPPIRASA"
     gene            complement(15654..16367)
                     /locus_tag="PLQ11_01515"
     CDS             complement(15654..16367)
                     /locus_tag="PLQ11_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HRK23611.1"
                     /translation="MNDVAQDAAVTKPRGMPTRAFRWVFSQSRLAWGLIAIAFFAFGI
                     LFWGGFNWAMELTNTEKFCISCHEMKTNVYNEYRNTVHYSNKTGVRATCPDCHVPKEW
                     GHKVVRKIQASNELYHKVLGSIDTPEKFNAHRLELSKNVWRAMKTTDSRECRNCHKFE
                     SMDYSMQEPRAAKLHQAGLNEGKTCIDCHQGVAHQLPPKAGVAYQDMLDNIDKVGTVQ
                     RIIDYLQGADVEKAKAAKR"
     gene            complement(16379..16837)
                     /locus_tag="PLQ11_01520"
     CDS             complement(16379..16837)
                     /locus_tag="PLQ11_01520"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: protein motif:HMM:NF015828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HRK23612.1"
                     /translation="MKHKADVLNVIAAAALLLGISVAGTGLAQDSRTKALREANLTDT
                     TPAPDILKQSTPAEGFSRAYRQQPPLIPHKVDTYQVTAENNQCMSCHDWPGNTRVNAP
                     KISETHYVDRQGVRLDKVAGTRYFCMQCHVPQADTKPLVGNSFQNATQIK"
     gene            complement(16874..17800)
                     /gene="napH"
                     /locus_tag="PLQ11_01525"
     CDS             complement(16874..17800)
                     /gene="napH"
                     /locus_tag="PLQ11_01525"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012605638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HRK23613.1"
                     /translation="MSANLIPYVEARSAKGWWQAHRFGIVRRLSQFAFLALFLTGPLF
                     GVWIAKGTLASSMTLGVLPLTDPLVLLQTLAARHWPELTAVIGAALVVGGYLAFGGRT
                     FCSWVCPVNPVADLAAWLRRRLGLTQTAKLRPSLRNWMIAAVLGASFATGIVVWELVN
                     PVTAVHRALVFGLWFGLLPVAAIFLFDLLTAKHGWCGHICPVGACYGVIGKAAVLRVS
                     AAARSRCDDCMDCFAVCPEPQVIGPALRGDKTGASPVILSGDCTLCGACVDVCPERVF
                     RFATRFDHRLDGATAPSPASPRPGGDPVAEAA"
     gene            complement(17797..18579)
                     /gene="napG"
                     /locus_tag="PLQ11_01530"
     CDS             complement(17797..18579)
                     /gene="napG"
                     /locus_tag="PLQ11_01530"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014286833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HRK23614.1"
                     /translation="MSAAGKTPKAPANPQRRKALEDMARFGGGALACGVMLSAFAEQS
                     RAKPAEALRPPGALPEKQFLSACVRCGLCVRDCPYGTLKLADWADGPALGTPFFHARN
                     VPCEMCDTIPCVKACPTGALDHSLIDINKSKMGVAVITSRETCLNLQGLRCDVCYRVC
                     PVIDKAITLERTHNNRTGKHAIFEPVVHAEFCTGCGKCEKSCVLTEAAIKVLPVEIAV
                     AAQDAHYRRGWVEKQKAGEALVDAPVKLEIRRPDAPPQGTPK"
     gene            complement(18598..21153)
                     /gene="napA"
                     /locus_tag="PLQ11_01535"
     CDS             complement(18598..21153)
                     /gene="napA"
                     /locus_tag="PLQ11_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase catalytic subunit NapA"
                     /protein_id="HRK23615.1"
                     /translation="MTKHVKPIEDQIAHDRRAVLKATAAAAAAASVGVTLPAEEALAQ
                     AGKTDGIRWDKGVCRFCGTGCGVIVGTKGGRVVATQGDPDAPVNRGLNCIKGYFLSKI
                     MYGDDRLTKPLLRMKNGQFDKNGDFTPISWDEAFKIMAEKWKAALKASGPSGVGMFGS
                     GQWTIWEGYAAAKLYKAGFRSNNLDPNARHCMASAVAGFMRTFGIDEPMGCYDDVENA
                     DAFVLWGSNMAEMHPILWSRITDRRLTHEGCEVHILSTFEHRSYELADNAMIFTPHGD
                     LAILNYIQNYIIKNGAVNKAFTEKHVEFARTVEDIGYGLRPTHPLEAKAVNASHKTGK
                     DGALDPAGMKDKIGFEEFAKLLEPYTLDYAHEKSGVPKDKLERLAKLYADPKKKIVSY
                     WTMGFNQHTRGTWVNNMIYNVHLLTGKISEPGNGPFSLTGQPSACGTAREVGTFAHRL
                     PADMVVVNPQHRAVTEKIWQLPEGTLPGNIGFHAVVQHRMLKDGKLNAYWVQCTNNMQ
                     TAPNMNEEGYPGYRNPANFIVVSDPYPTVTAMAADLILPTAMWMEKEGAYGNAERRTQ
                     FWRQQVKAPGEARSDLWQTMEFAKHFKMEEVWPEELLAKKPEYRGKTLYDVLFANGRI
                     NKFPLTETQKGYDNDESTHFGYYVQKGIFEEYAEFGRGKAHDLASFDVYHQTRGLRWP
                     VVDNKETLWRFKEGYDPYVKAGEGFSFYGKPDKKARAIFCPFEPAAEIPDKEFDLWLC
                     TGRVLEHWHSGSMTRRVPELHRSVPSAVLYMHPDDAAERKLNRGQTVKIQTKRGELIT
                     RVETKGRNKPPKGLVYFPFFDESQLANKLTLDATCPISKETDFKKCACKVVTA"
     gene            complement(21157..21501)
                     /locus_tag="PLQ11_01540"
     CDS             complement(21157..21501)
                     /locus_tag="PLQ11_01540"
                     /inference="COORDINATES: protein motif:HMM:NF015861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HRK23616.1"
                     /translation="MTIASQSDALNVVGCVVQTAPGKASSVRENLIGLPGVDVHAEAE
                     DGRLVITILDTDSGMAIDQLAAINRMPGVVSTMLAYHGIDHSHPVEDGSSAGCSTCSS
                     SDRTSSRFSERA"
     gene            complement(21498..21740)
                     /locus_tag="PLQ11_01545"
     CDS             complement(21498..21740)
                     /locus_tag="PLQ11_01545"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRK23617.1"
                     /translation="MTAACVEPKGVTCRRCGDECEAGAIRFRLSGRGKADVLVDQALC
                     TGCGACLSVCPTNGLELVNADRATFVAGLVALEAGQ"
     gene            complement(22071..23864)
                     /locus_tag="PLQ11_01550"
     CDS             complement(22071..23864)
                     /locus_tag="PLQ11_01550"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF and EAL domain-containing protein"
                     /protein_id="HRK23618.1"
                     /translation="MAVPEVSGAFGMDHLDRRVLGVFVDQTEQQSSPALASPSNAFDP
                     RTILSSVGEAVYSWDIETDRMTWSQNATEVLGINDIAQITSGSGFAVVTDSASPTSLQ
                     ATIFECQRQDKGTGVAYSARYLFNPTPGRRIWIEDKGRWFANAAGRPWLAHGVVRVVS
                     AASDAEAAIATTRQIDPATGALTRPEFCNCLGEALEKVRKDKRHLALVIFGIEDLAHH
                     NKAYGYNVGDELIAAIARRIRSHVRKHDVIGRYAGNKLALLLATTIPDDLERTVSRIA
                     QVVRDRPIDTSAGAISASVKAGTVAAPRDVNDVAEILTAAEEALADAKADANCVVAIW
                     TKDAHRTANRKQNAAQTDEVLSALNERRIQLAFQPITHAKGGGIAMYESLVRMRSRTG
                     QLVGAGEIIPVAEKLGFVHLVDHRVMELAFATLQERADVTLTFNAGVSTVLHPEWMHT
                     FKSLIGAHPDVAPRMIVEITETSLIEDLSVATKVIESIKALGACVAIDDFGAGHTSFR
                     NMRMLPIDILKIDGTFIKNLMQSSDDRFFVRTLLQLASHLNIRTVAEWVQDQESAKLL
                     ADWGVAYLQGDFIGDPLNELPPAPSVVAASA"
     gene            complement(24199..24390)
                     /gene="rpmF"
                     /locus_tag="PLQ11_01555"
     CDS             complement(24199..24390)
                     /gene="rpmF"
                     /locus_tag="PLQ11_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HRK23619.1"
                     /translation="MAVPKRKTSRMKRGHRRSHDALAAPAYVEDKNSGELRRPHHVDL
                     KSGMYRGRQILKPKVEAGA"
     gene            24736..25590
                     /locus_tag="PLQ11_01560"
     CDS             24736..25590
                     /locus_tag="PLQ11_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRK23620.1"
                     /translation="MAALLHSGNRRPERLMAAMRHGTLAGGKRLRPYLVLNSARLCGQ
                     PEGWARIIAMALECIHCYSLVHDDLPAMDNDDMRRGRPTVHRAFDEATAILAGDGLLT
                     MAFSLLASADAPGDPATRLHIIAALADAAGIDGMVGGQMLDLAAEGRFADLAEPLDLA
                     AVSRLQALKTGALIRFAAMAGALMTGGRQPDHLAALDRYGTALGLAFQIRDDLIDVEV
                     DSATAGKATGKDDDAGKGTFVSLLGLDGARARLEAVTHEGLSALAPFGAAAGPLAAAL
                     TFNQTRRA"
     gene            25663..>26775
                     /locus_tag="PLQ11_01565"
     CDS             25663..>26775
                     /locus_tag="PLQ11_01565"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRK23621.1"
                     /translation="MKKLLGLLLAGVAVAGTVWAFLGHDNGTPATASYRTASAERGTI
                     IAQVTTSGAIAPVTTVIVGSQLSGQVIEILADYNSEVKNGQVLARLNSDQIRAKLDGA
                     RADLAQARASRVMLDAQLEKSRADAQKAAANADDMGHQLEKTETQLADAEKTLARQTS
                     LNAQGIVATAALQTAQTQRDTLRAARESAHAQIASAKAQIAALAADQKMIAAQMVSAE
                     AQIAQRSAVVRQIEVDLGNTDIRSPVDGVVVQRNVELGQPVAASLQAPTLFLVAQDLR
                     QVQIQANVDEADVGRVRDGQPVSFTVNAYPGRTFQGKVKLVRLGSQTVQNVVIYTTVI
                     DVDNPDIALKPGMTANLRIVTEQRDNVVRVPNAALRW"
BASE COUNT         4871 a         8747 c         8220 g         4937 t
ORIGIN      
        1 agcgggcgct ggccgaagcc gctgcccgcc gccgcgcgca ggaggagcgc gaagccgtgc
       61 tgaagcgtca gcgcgagatc aatggccgcg gcggcctcga tccggtccgt tacgaggatt
      121 gggaggtcaa ggggattgcc agcgatttct gatcgcggaa aataaaaatc acaacctgac
      181 gctgcgtcag gaagttcaca atttgttcgc gacttgctgg ataggctgca tctgatccct
      241 tgacggagcg ggacgatgcg cacctcatcg ctgtttgccc ttgtgttgct gcttgcgctc
      301 tgcggcagcg ctctgagccg ccgacctgct gcagagacgg caatggtggc cgccagccgc
      361 ggtgtgaatg ctccggttgc cccaggcagc ctgcccgcca cgaggccgga tcgttcgcgc
      421 ccgctcctgc aaggcccgct cgccgccgag cttctttccg tcattgatgg agatacgatc
      481 gaggtgctgg tgcagacctt catcgatcag acgacccggg tgagggtcag gctgcgcggt
      541 attgatgcgc ccgaactgtc caccgcctgt gccgaggagc acgatcttgc acaggccgcg
      601 cgcaatgcgc tggccggcct gctggcgggc ggacgcctca tcctgaccga gattggccgc
      661 gacaagtatg ccggtcgcgt tgtggcgcgc gtgctctccg tcaacggcga ggatgtcggc
      721 gcccgcctgc tcgccgaggg ctacgcgctt gcctatgccg gcggacgccg gcagccctgg
      781 tgcgacacca gtcttaccgc actacggtga cggtcgtgcc cagggaaacc cgctcgaaca
      841 gctcgacgat atcgtgattg aacatgcgga tgcagccata ggatgccggc cggccgatcg
      901 agcccggaac attggtcccg tggatcgcat attgccccgg tgacagggtg atcgcccgct
      961 cgcccatcgg attggaggga acgccgcccg gaatgacatt cggcagatgc ggattgtcgc
     1021 gccgcacatc gtcgggcggg ctccaggccg ggcggacata tttgccgtcg acgacgcgct
     1081 ggccaaacca ctgcttgccc gggcgtccga cggcgaccgg atagcgcagc gccgatccat
     1141 cgccgagcac cagatacagc cgccgctgcc cggtcgaaac gatgatcgac ccagccgaat
     1201 aagcgcctgg cagcggcacg cgctcgcgcg cttcggcact gaacgacact ccaatcgccg
     1261 gcatggccaa aagaccaccc accagcgtac gacgcgacag actactcgac atgacacacc
     1321 cttgaggcta cacggggtcc tctgagcgga tcaggccagt ctggcggttc ataacaaatg
     1381 aatcaagatc ggtcggctga tcttaacctt acgaagacgt gaagccgccc cggggctgca
     1441 ccgccccgtc gcttgacgca gaacaaggtg gcacgcgcct tgatatgaca ggcagagccg
     1501 atgagcgcac ctgaaaccct cgccctttct gccgaaacca tccgcgccct ggccaccccg
     1561 gtgccgcgtt ataccagcta tccgaccgcc ccgcatttca acgccggcgt ttcggcggcc
     1621 gattatgcgc gctggctcag cgaattgccg gcgcaaagcc ggctgtcgct ctatatgcat
     1681 atacccttct gcgataccct gtgctggttc tgtggctgtt cgaccaagat cacccagaaa
     1741 tacgcgccgg tctccagcta tctcgacctg ctggtgcgcg agatcggatt gatcggcacg
     1801 ctggtcccct ccgatgcccg cgtggcgcac atccattggg gcggcggttc gccgaccatg
     1861 ctcacaccgc cggacattgc ccggctggct gcggcgaccc gggcggcctt caagctggac
     1921 atcgactgcg aatttgccat cgaggtcgat ccacgcggcc tcgatgtcgc ccatatcgac
     1981 gcgctggcca aggccggcct gacccgtgtt tcgatcggcg tgcaggattt cgacaggcag
     2041 gtgcagcagg cgatcaaccg ggtgcagagc ttcgaggaga cgcgcgctgc ggtcgagggc
     2101 ttccgcgccc gcggcatcac ctcgctcaac atcgatgcac tttacggcct gccgcaccag
     2161 accacggaac ggatgatcga gaccctccgg caggtcatgc tgctcagacc ggaccgtatc
     2221 gccctgttcg gctatgccca tgtgccgtgg atgaagcgcc atcaggcgat gatcgacgag
     2281 gctgccctgc cggatgtggt ggaacgctat caacaggccg aaaccgccgc ggcctatctg
     2341 gtcgcccatg gctatcagcg gatcggcttc gaccactttg cgcttgcgac cgattccatg
     2401 gcgatcgcgg cgcgcgatgg cacgctcaag cgcaatttcc aaggctatac ggtcgatccg
     2461 gccgacgcat tgatcggcct cggcgccacc gccatcggcg ccctgccgca gggctatgtg
     2521 cagaatgaca ccgccaccgg ccgttacatg acggccatcg gcaagggcat ggtcgccacc
     2581 gagaaaggac tggctctctc tgctgaggat cgcctgcgcc gtgcggtcat cgagcggctg
     2641 atgtgcagcc tgaccttttc ggcggcagaa gtgagagcgc ttgctccttc ggcagacctt
     2701 gcccggacgc tgatcgagga ggcgcggcag gttgtcgccg aggacgaata cggatttgtg
     2761 gccgccaccg gcgatggctt tgtcatcacc gaggccgggc gcccgttcat gcgctcgatt
     2821 gccgccaggt tcgatgccta tctgccgcgc cggaccgcgc gccattctgt cgccgtctga
     2881 tcagggcagg tccgcgttat tcaaccatgc cggcagggct tcgatgccgt cgagcaccag
     2941 atcggcatgg ggggccagtt cctcgcgact ggcaaagccg gacagcaccc cgatcgccag
     3001 gcatcccgcc gcccgcgcgg ctgccagatc atgggttgtg tcacccacca tcgccatacg
     3061 cgacggaacg agcccgaacc gttcggcaaa ggccaggatc tgaccgggcc ccggcttgcg
     3121 cccgaagcct gaatcgtagc cgataatcgc atcgaggcgc gccgtcagtt ccagcctgtc
     3181 gcattgggcg cgggcgcccg cttcggtatc gttggtgatg acgccgagcc taagcccccg
     3241 ccctctcaac cggtcgaaca ccgcgcccgg atcaccgatc ggggtcacat gagccaacgc
     3301 cgccgcatca agcaggcgat ccatcatccg gtggaaatcg gtgtcgaaag acacccccag
     3361 cgcccgcgcc cagccggcgg caaaatcggc tgaagcctgt gcgataaatg gcgaatccgg
     3421 atagagccgc ccggtcgacg gttcgaaccg attgaaagtg gccagccgct gataggctgc
     3481 ctcatccccc ttggccaggc tgcgcatcac gctgtcgatt gcgggtcccc aggtcgccga
     3541 gaagtcggca aacgtcccat ccttgtcgaa cagaatggcc gccaacttca ccgccgagcc
     3601 ctcgccttaa ccgcttggtg gcgcatagct ggcgctgttg ccgtcatgtt cggccacatc
     3661 cacccgctcc agccacaccc gcccgcgcgt cttcttgtcg atgaaggctg cgacatgcgc
     3721 cgccgccagt tccgccacag cctcacaccc tacccttggc agcacccgga ggtcgacgat
     3781 cttgcgccgg gcgaggtcct gaaaggtctc caggtccgga tcgtcctcgg caaccaggac
     3841 cgtatgatcg aacatcgaat gcagccaggc acgcacctcc ttcatcccgc cgaaatcata
     3901 gcaccagttg ttggcgtcga gccggtcggt gacaaaatgg aaggtgaagg ccagcgcata
     3961 gccatgcagg aaagtgcaat gcgaatgtgt tgcccgccat tgccggaagc agcaggacaa
     4021 gccttcgctg tggccgaact gcttgacggt gcgatagagc gccacgcgcc ccgcagcttc
     4081 gccatcccgg ctcatccacg cggtcccagc gtcgcgcatt cctgcaggaa ctcggcccgg
     4141 agcgccgcat tgcggtcaag ctcgccgtaa tagctggtgt tcaccatccg gctcgcatgg
     4201 gaagcccgca cgccgcgatg ggtcttgcac atgtgcaccg cgctgatgcg cacggcgagg
     4261 ccgcgtggat gggtcttttc gacgacaaaa tcctggatct gcttgaccag ctcttcctgg
     4321 atctgcaagc gcgccgcgaa atattggaca atccggtcat atttcgacaa gccgatgatt
     4381 gtcccgtccg gcgagggcag cacaccgatg aaggcatgcc cgtagatcgg catcatgtgg
     4441 tgcgcgcagg tcgagcgcac ctcgatcggt ccggtgacga tcagatcgtc gaaacagcgg
     4501 acattctcga attcggtgat cttcggcggt gtctcgtagc gaccggcgag cagctcctcc
     4561 accagcatct tggcaacccg ctgcggtgtt tcgcgcgtgt tatggtcgtt gcgatggtcg
     4621 atccgcagaa tgtcgaacaa ctcctcgatc ttctgcgctg cggcggcctt gagtgcctcg
     4681 cgttccgccg ggcgggcata gccatccacg gcctcattgc acaggcgcgc cgccgtcatc
     4741 ctgagaatgt tcgacttcat aaccactcca gacatgcgcc caagacataa ccccgaggcc
     4801 ggctgtcccg gcccgactac cacaacagtc acaccggacc aaagccaatt cgccacggct
     4861 gatcgctgcg acactctgga tgtcggtgcg cttaatcaaa ctttcaagct ttcgcgcaac
     4921 ccttggtgca caacgttgcc ttcttcgccg ccacgcgcga ccggcagcgt caacggctga
     4981 cacccggcgc gacgggtgtg gatggatcga tgaagcgggt tcgtatcgta cgtttggtga
     5041 gcgcgattgt cgcgctcctg ctgttggccg gcgcaggcta ccagggcttc cagatccgtg
     5101 cgatgaaaca ggacagtgct gcatcttcgc atattgcact ggcgcgccag agtgcggatg
     5161 ccagcgctga gctggccagt ctgatcggca aggtcggcga actgggcgtt gcggcgacgg
     5221 cctatcgcga ttcatccggt cttgccggtg tgcgcgcctc catggccagc ctgcagcagc
     5281 accagaagga actcgccgcg ccaatccaca aagcgttcat cgccgcagac agcacccacg
     5341 cacgggtccc ggccgatctt gcggcgatgc tgacacagtt ggacaggcgg ctgggcaatc
     5401 tgactgagcg ccgattcgtg ccggaggtgc tggagctgct gtccgccatg gaacgcccgc
     5461 tgctgggtct tgcccatgct gccgccgaac actccagccg ggccctggag gcggagcgca
     5521 gcctgttgac cggcatgaac catcggctca tggcgtggtt ggccggtctt gctttggcta
     5581 tgatggtgtt tctgggcgcg ctgctgtccg aactcaacgt gctctatcgc aacaattcgc
     5641 agctcaatgt ggtcgctggc agcctcgaaa agaccaaggg agacctcgaa actgccaaca
     5701 agagcatcca gaacgcctat gccgaactga atctgcacaa ccagatcctg aaagtgcatg
     5761 acaaggagat gcggacgcag aacctgaggt tcgacgccgc gctcaacaac atgacgcagg
     5821 gcctgtgcat gaccggcgcc gatggcaagc tgatcgtctg caacgaacag ttccgccagt
     5881 tgttctcgat cgacaaggag accgcccgcc ccggcaccga catgaccgcc attctggtgc
     5941 gcatgggcgt tgcgctcaaa ctgggcgaga ccggcaccgc cgaatggatg cgaacccacg
     6001 atacgctggt cgcggctgcc aaacacgagg ctttcacccg cgactgcccg gatgggcgct
     6061 ccttctccat tcaccaccag ccgatgacgg atggcggatg ggtggaaacc tatgaggaca
     6121 tcaccgagcg ccgcaaggcc gaggaaaaga tcttccatat ggcgcatcat gatgcgctca
     6181 ccggcctgcc caaccgcgtc tatttcatgc agcatctgga agaagcgctg ggccgggtgc
     6241 gccgcggcga agccgcctgc gccgtgttgt gtcttgacct cgaccggttc aaggcggtca
     6301 acgatactct gggccatccg gtcggcgacc tgctgctcaa ggaagtcgcc gcccggctgc
     6361 gagctgcggt gaatccttcg gatgttgtgg cacgtttcgg cggtgacgag ttcgccatcc
     6421 tgaagaattc cgatgtcgaa ccggaggctc tggcgggtct tggcgaaaag ctggtcaaga
     6481 gcatctccgc cccgttccat cttgatggca acgaggtcac ggtcggcacc tcggttggca
     6541 tcggcatcgg ctttcaggat ggggccagca ccgagcaatt gctcaagaat gccgacctcg
     6601 cgctgtatca ttccaaatcg gaagggcgcg gcgcctatca cttcttcgaa gcgcatatgg
     6661 atgccaaggc acaggcccgc cgcctggtcg agaccgatct caagcgcgcc atccctgccg
     6721 gcgaactcga ccttttctat cagccactcg tcgatgtcat gacccgccgc atcgtcggct
     6781 tcgaatcgct gatccgctgg cgtcatgcgg aaaagggcat gatttccccg gccgaattca
     6841 tccccatcgc cgaggaagtc ggccttatca tccagattgg cgaatgggca ctccggcggg
     6901 cgtgcgccac cgcgctgctt tggcccgagg acatcaaggt ttcggtcaac ctgtcgccgg
     6961 tgcagttccg cgaccgcaat ctcgtccagg tggtcaagaa cgttcttgcc gaaaccggcc
     7021 tcgcccccca ccggctcgat cttgaaatta ccgagacagc cctcgtccag gacatagact
     7081 tcacggtgaa ggtgctgctg gcactcaggg aaatgggtgt tcaggtgtcg ctcgacgact
     7141 ttggcaccgg ctattcttcg ctcagctacc tgcgctcctt cccgttcgac aagatcaaga
     7201 tcgaccagtc gttcgtgcgc gatcttgcca cccgtccgga ttgcctcgcc atcgtcaaat
     7261 cggtcaccac gctcggcgag aacctaggca tgaagaccct cgccgaaggt gtcgaggaag
     7321 aggagcatct gacgatcctg cgcgagctgg gctgccagga ggcgcagggc tacctgttca
     7381 gccggccggt tccgaacccg gaagcgttga agctcatcga gacgttcaat ggcgtcacca
     7441 ttgcagcgcc ggccgcagcc aaggctgcag tcgccgcctg accgtttgtc ggaaccacag
     7501 cgcgccgaca gacagtgtga tcgttatcaa tcatgtagag cgtgcctatc gcgattaacc
     7561 ggcttccact gaagcgcagc atgctctaga cctccgccac cgcgtatctg ccaacaggtc
     7621 gcaagaggtc gttcatgtcg ctgttccgtc tctatgccag ggtgattgcc cagctcggca
     7681 acgagttgcg catgggcatg ctgctgatgt tcgccaatct tgtgctggcg atcgcccagt
     7741 ttgccgaacc ctacctgttc ggccggatca tcaatctgct gaccggaaca tcgggcggca
     7801 ccaagccaac cttggatgca ctgatgccgc tgatcatcgc ctggctggtg tttggcctgt
     7861 tcaccatcgg tggcggcgtt ctggtcgccc tgcatgccga ccggctgtcg catcgccgtc
     7921 gcctcggcgt gatggccggt ttcttcgaac acgtgcttgc cctgccgctg gctttccatt
     7981 cgcagaccca ttcgggacgt gtcctgaagg tgatgctgga aggctcaaat ggcatggcct
     8041 ggctgtggct gggcttcttc cgcgatcatt gcgcagcgct gatcagcctg ttcgtgctgt
     8101 tgccgttcac cctctttgtc aactggcgcc tcggcctgct gctctgcgtg ctggtcgttg
     8161 tcttcgccgt gctgaccgcc atcgtgctgc gcaagaccga aaccaaacag ggcgctgtcg
     8221 agcggtatca taccgacctg gccgaacacg cttccgacgc cttcggcaac atcccggtga
     8281 tccagagttt cacccgggtg caggccgaac tgcaccattt gcgcgacatc atcgaccggt
     8341 tgctgactgc tcagatcccc gtgctgtcct ggtgggccct ggcctcggtg gcgacccgcg
     8401 ccgcctcgac gctgaccgtc accgccattt tcctgctcgg cacctggctg cattaccagg
     8461 atctcgccag catcggcgac atcgtcacct tcatgggtta cgcgggcctg ttgatcggca
     8521 agctggaggc cgccgtcggc ttcgtcaatt cgctgttcat gcaggcgccg aagatggagg
     8581 aattcttcga ggtgctcgac accaccccca gcgtgcacga caagccccac gccatcgaac
     8641 tcagccgccc ggaaggcgca gtgcgctttg aatccgtctc gttttcctat gatggcaagc
     8701 gcgcggcgat caaggatgtc gagttttcgg tcgagcctgg ccagacgata gcgctggtgg
     8761 gcgccactgg atcaggcaag tcgaccacgc tcggcctgct gcaccgggcg tttgatccgc
     8821 agtcgggccg tatcaccgtg gatggtcatg acatccgcga tgtcacactg ttgtcgctgc
     8881 gccgcaacat cgccgtcgtg ttccaggagc cgatgctgtt tgcccgttcg atccgcgaaa
     8941 atctccaggt cggccggccg gatgcgacgg acgaggaaat gatggaggcg ctcaagcgtg
     9001 cccaggcgct tgaattcatc gaacggcagg acgccggtct cgacacggtc attggcgaac
     9061 gcggccggtc cctgtccggt ggtgagcgcc agcgcctgtc gattgcccgc gcgttgctga
     9121 aggacccgcc gattctcatc ctcgacgaag ccaccagcgc attggacgcc accaccgagc
     9181 gcaagctgca ggctgcgctc gacgaggtca tgaagaaccg caccactttt gtcattgccc
     9241 atcgcctcgc caccattcgc aacgcctcgc gcatcttcgt gttcgagcgc ggcgaagtgg
     9301 tcgagaatgg caacttcgag caactggtgg cactcaatgg tcgctttgcc acgctggccc
     9361 gcgcccagtt catggctggc agcgaccagg cagcgcagac ccctgcctcg gccgatccgg
     9421 tggtttacgg ctgaggcgtc cgctcgttca actcatcggt gaggaaggaa acgaccgccc
     9481 ccggatcgac tgtcctggca atgaagctct ggccgatccc gcgcgtcagg atgaaggtga
     9541 gcgccccgcg cgaaactttc ttgtcctgcc ggatcgccgc catcagttcg tccgccgaac
     9601 cgaccccgcc cggcacatcc tgaagccgcg tcggcaagcc gaccgcctgc aggtgccggc
     9661 tgacacgcaa ggcgtcctgc cctgtgcaga gcccaagcct ttgcgaaaag cgatgcgcca
     9721 gggcaatgcc gatggccact ccttcgccgt ggacaagccg ggccgaattg tagccggtca
     9781 ccgcctccag cgcatgaccg aatgtatggc ccagattgag cagggcgcgg tccccggtct
     9841 cggtttcatc gcgcgccacc actgcggcct ttgccgcgca ggaaatgcgc acggcctctt
     9901 cacggtcggc agagccgtca agaatgccgc gccaatgccc ggcctcaagc caatcgaaga
     9961 acgccggctg gtcaatcagg ccatacttca ccacttcggc atagccggcg cggaactccc
    10021 gcgccgacag cgtatcgagc aatgccgtgt cggcgagcac caggatcggc tggtggaagg
    10081 ccccgaccag attcttgcca tgcggagaat tgacggccgt cttgccgccg accgaggaat
    10141 cgacctgcgc cagcagcgtc gtcgggatct gcacaaaggc catgccgcgt cgcacgatgg
    10201 ccgccgcaaa gccggcaagg tcgccgatca ccccgccgcc gaacgccacc acgatgtcgc
    10261 gccgctccag tcggtcagcc agcagggctt cgacaacatg gccgagctcg gtccagttct
    10321 tggtcgcctc cccctgtttc agcacgaggg tgcgcgctgc aatgccagcg gaagccaggc
    10381 ttcgggtgag gcggtcaagg tgcaacccgg ccaccgtctg gtcggtaatg atcgccgccc
    10441 tcgcccccgg caccagccga gcgatctcgt cgccggcagt atcgatcagg ttccggccga
    10501 tgcggatgtc atagctgcgc ccggccaggg caaccggaac agtgaagaac ggggcggaca
    10561 tcagctttgc tccggttgcg cggcaaggtg gtttgccagt gctgcgacca ccacatcgac
    10621 catctcttca tgcggcacat cggcggacag cacggtgata tcggccagcg catagacggg
    10681 atagcgctcg gcgatcagct tgcggacagt ggcctcggga tcgtcggtct tgagcagcgg
    10741 ccggttcgac cgccgtctta cccgccgcat gatgacgtcg aaatccgcct tcagccagag
    10801 cgagagaccg gcttcgcgga tcgcctgccg ggttgcctcg ttcatgaaag ccccgcctcc
    10861 cgtcgccagc acctgtggcc cctgcttcag caatcgggcg atgacgcggc gctcaccggc
    10921 ccggaactcc gcctctccat gcctggcgaa gatgtccgga atactcatgt ccgctgcttc
    10981 ctcgatggcg tcatcggcat cgatgaacgg cagatccaga aactggccaa ggcgcttgcc
    11041 gatcgaggtc ttgccggcgc ccatcatccc gacaagtacg atcgaccgat cgccgagtcg
    11101 ctcgacgatg gtccgcccct gcgctgcggc cgtcaccggg acggcatccg gctcattctt
    11161 gctcatggcc tccggcactt caccaacaca acatataagc cattgaattt tatagtattc
    11221 tcaatgcact gacgacccca gcgccatgac agggaggtct agcacggata ccggcgaacg
    11281 caaatcgcaa ccggctcccg gcgcgctttc ggcgaaaaag ccgttgccac tcttccgcaa
    11341 gctgctctag ggttgcaccg gaagaggtgc ctgtgctcgc catcgtgcga cttcttgtgt
    11401 ttttcagcgt gctggcggcc attggctttg gctcgatgat tgcgcttgtg caatttgtcg
    11461 aacctgaaca gcgtgactat tccatcgtgg ttccgccgtc gaaattcaac aagtagtgcc
    11521 atggcgcccc gcgtttcccg ccgcacggcc attgatgatt ttctcgacat gatggctgcc
    11581 gaacgggggg ccagcatcaa tacgctggcg gcctatcggc gtgatctcga tgattatcgt
    11641 gcctggctgg cggccaccgg gcacgatgaa gccagcgtcg tcaccaatgc catccgcgcc
    11701 tacctcgccg acctggccgg tcgcgggctg aaggcctcca gcgctgcccg caaattgtcg
    11761 gcgctgcgcc agtttcacgc cttcctctat cgcgaaggtc gccgcagcga tgatccgacc
    11821 gtcatcatcg agggccccaa gcgcagccgg ccgctgccga agagccttgg ggtggacgat
    11881 gtcgatcgcc tgcttgccgt tgcggccgaa ggctgcggcg acgcgtcccg gccgcttgcc
    11941 gatcgcatcg cggccgctcg gctctctgcc ctgctcgaac tggtctatgc aacgggtctg
    12001 cgcgtatccg aactggtgac cctgcccacc gaagcggtgc ggtccggcag ggataccatg
    12061 atggtgcgcg gcaagggtcg caaggagcgt ctggtagtgc tgtcgccggc cgccatcgcg
    12121 gcggtgcagc gctatcgcga cctgaccacc ccgaaaggcg gcccgcgccc gaagttcctg
    12181 ttccccgcag agggagccgc cgggcatctt tcgcgtcagg tctttgcccg cgacctcaaa
    12241 cgctgtgcag cagccgcggg tctcgatccc gagcggatca gcccgcatgt tctgcgtcat
    12301 gcctttgcca gccatctgct gcagaacggt gccgatctgc gcatcgttca gcaattgctg
    12361 ggccatgcgg atgttgccac gacgcagatc tacacccatg tgctcgacga gcgctcgaga
    12421 tcgatggtgc gtgatctgca ccctcttacc gatacggatg accgctgagg caacgcaccg
    12481 gctttccctt ccccgcggct tcctgtaagg acgctggcgg atctggcaga cgcccgagac
    12541 ctgtcgcaag cgaagagtgt cgatgcgcta ttttgatttt gaaacctccg ttgccgaact
    12601 ggagaacaag ctggccgaac tgaaggccct cgccagcacc ggcggcaaca gggatatcgg
    12661 cgatgatgtg cgcaagctgg aaggccgcgc gcacgacatg ttgaagacgc tctacgccag
    12721 cctgacgccc tggcagaaga cgctggttgc ccgccaccag cagcgcccgc attgcattga
    12781 ctatatccgc gaactgatca ccgacttttc gccgctggcc ggcgatcgca gctttggaga
    12841 ggatgaggcg atcattggcg gcttcggccg cttcaacggt cagtcgatct gcgtgatcgg
    12901 ccaggaaaag gggacggaca ctgaatcccg cctgcgccat aatttcggca tggcccgccc
    12961 cgaggggtat cgcaaggccg tccggttgat ggagatggcg gatcgctttg ccatcccggt
    13021 gctgtgtctg attgataccg ccggcgccta tcccggcatt gacgccgagg aacgcggcca
    13081 ggccgaagcg atcgcccgtt cgacggaggc atgccttgcg cttggcgtgc cgaacgttgc
    13141 tgtcatcatc ggcgagggcg gctcaggcgg cgctgtggct attgccaccg ccaatcgcgt
    13201 cgcgatgctg gaacattcga tctacagcgt gatctcgccc gaaggcgccg cgtcgatcct
    13261 gtggcgggac agcgcccgtg cccaggatgc agccagcacg atgaagatca ctgcccaaga
    13321 tctgctcaaa ttcggtgtca tcgataccat tatccccgaa ccggtcggcg gtgcccatcg
    13381 cgatccgaaa gccgcgatca acaatgccgg agcggtgatc cagaccgcac ttggcgagtt
    13441 ggtgcaacgc ggcccggagg aattgcgtac ggcgcgggcg gagaaattcc tcgccatggg
    13501 gcgcaagctg taacggcggg ctttgttaac aggccgttga cattgtggcc cgaattcatg
    13561 gcgttgtggt aacctttggt gaactctgac ggccataaag ataggagctg tcactccgtg
    13621 cgttattgga tcatccctcg atgagactat cccgttggat tcggcctggc gccgcgcttg
    13681 ccgcagttct cctccttgca ggttgcgatg aggatggacg cctgtcgcgt aatgcccggc
    13741 attacacgcc gatcccggct cccatggtcg cgttgatgag cgagaagggc acgaacaagc
    13801 atgctcctgt cgtgctgcgt gccttcaaga aggaaagcga actggaaatc tggaaacaga
    13861 atgctgccgg cgaatatatc ctgctcaaga cctatccgat gtgccgctgg tccggtcagc
    13921 tggggccaaa ggtgcgtgag ggtgaccgtc aggtcccgga aggcttctat tcgatcacac
    13981 ccacctcgct caatcccaac tcgtcgttct atctgtcgtt caacgtcggc tatcccaacg
    14041 cctatgaccg cgcccatggc cgcaccggat cggccatcat ggtgcatggc gcctgttcgt
    14101 ccgccggctg tttttcgatg accgacgagc agatcgccga aatctacgcg atcacccgcg
    14161 aggcatttgc cggcggccag aaggctatcc agcttcaatc ctatcctttc cgcttcacca
    14221 cccagaacct ggccaagttc cgtgctgatc cgcatatgcc attctggaag atgctgaagg
    14281 aaggttcgga ttcgtttgaa ttgagcaagc aggaagtgca ggtcagcgtc tgcgaagccc
    14341 gctatgcgtt caatcgcggt caacgcaccg acggcagccc cgtctgcggc ccgcaggatg
    14401 ggacctcgcc gcttgtccag gcggtcgccg ccaaggctcg ctccgacgaa cagaaggttg
    14461 ccgagcttgc cgccagcggt gtcggcgccg ttcgccggat ctacaaggat ggcgatcagc
    14521 atccggcctt ccgcaccacc cattttgccg ccaccgattc cgaaggccgg accatcgcct
    14581 ccccgcgctc ttctgcctcc cgtgtggccg aggtcagccg ccccgacgcg ctggatcatg
    14641 gtccggtcga ggttgccgtc gattcgctga aaggccgcgg gcaggccccg gcagcagcgc
    14701 tcgcccgcac caacctggca acccaggcgc cggccctgcc gtctccgccg cccgttgttg
    14761 ctgcgccagc gacgccggca gcacccagcg ccaacgcctt ttcgcccacg gtcaccggca
    14821 acagctcgat tttccgaggc ttgctgggcg gctcgcccga accctcccga ccggatgtcg
    14881 tgacggctgt ggaaccggac agaccgcagc ctgtccgcat cccgctgcca ccgcgcgccc
    14941 cgcagcgtca ggctggtcag ccagccgcac cgcttgaagc ggctccggca gaaatcccga
    15001 accagccatc cgcctcgttc cagacggtac tcaagggctt caacgccctg atccccggca
    15061 tgcgctgaag acgacagtcc gcgccaggca gaacagaaat cagaaaagcg gatcaggcgc
    15121 ttgcgcggat tggcggaggc ggatcaccaa tccatgccag gatggccgct gcatccggct
    15181 ccagaacatc ggcctcgcct ccggtgaact ccatccaggc gggcaggttc gctgcactga
    15241 cgccaaccag caccggtatg ccctgcagga cagcttcggc gatcgccggg cgaaatccat
    15301 gcccggccgc ttcggatttc ccgaacttgt tgagaatgag cagatccgct ccgcccgcca
    15361 gcccggcctc taccagcgag acaacctcgg ccatcgcgcc cgggtccagc cggcagccgc
    15421 gcgcatactg gcccagccgt tgcgaaatgc ggatggaacg accgctgttg agatcgctca
    15481 gcgtcatgtc gcaggcgcag agcgcatcgt agcggtcgtt ggtctgcacg gcgccggcaa
    15541 gccgataccc ctccccagca agctgccgtg caacatcggc gagcagggcg tcgcacgacg
    15601 ccccttcgcg atagagcacg gcagcgagcg atgacgaacg catcttaccc tcttcagcgc
    15661 ttggcggcct tggccttctc cacgtccgcc ccttgcagat agtcgatgat gcgctgaaca
    15721 gtaccaacct tgtcgatatt gtcgagcatg tcctgatagg caacaccagc cttgggcggc
    15781 aactgatgcg ccacgccttg atggcagtcg atgcatgtct tgccctcatt caggccagcc
    15841 tggtgcagct tggcagcacg cggctcctgc atcgaataat ccatcgattc gaacttgtgg
    15901 cagttgcggc actcgcgcga gtcggttgtc ttcatcgccc gccagacgtt cttggacaat
    15961 tccagccggt gagcgttgaa cttctcgggc gtgtcgatcg agccaagcac cttgtggtaa
    16021 agctcgttgc tcgcctggat cttgcggaca accttgtggc cccattcctt cggcacatgg
    16081 caatccggac aggtggcgcg cacaccggtc ttgttcgaat aatgaacggt attgcggtat
    16141 tcgttgtaga cgttggtttt catctcatga cacgagatgc agaatttctc cgtattggtg
    16201 agttccattg cccagttgaa cccgccccag aacaggatgc cgaaggcgaa gaaggcaatg
    16261 gcaatcaaac cccacgctag cctggactgg gaaaagaccc agcgaaacgc gcgggttggc
    16321 atacccctgg gtttggtgac agcagcgtct tgtgcaacat cgttcatgac gccccccgtt
    16381 acttgatctg ggttgcgttc tggaaggagt ttccaaccag aggcttggtg tccgcctgcg
    16441 gcacatggca ctgcatgcag aagtagcggg tccccgccac cttatcgagg cgcacgccct
    16501 ggcggtcgac atagtgggtt tcagaaatct tcggcgcatt gacgcgggtg tttcccggcc
    16561 agtcatggca gctcatgcac tggttgttct cggccgtgac ctgataggta tcaaccttgt
    16621 gcgggatgag cggcggctgc tgccggtagg cacggctaaa cccctcggct ggtgtcgact
    16681 gcttcaggat atcgggagca ggcgtcgtgt cggtcaggtt ggcctcacgc agggccttgg
    16741 tcctgctgtc ctgggcaaga cctgttccgg ccacggaaat cccgagcagc agcgccgctg
    16801 ccgcgatgac gttcaggaca tcagctttgt gtttcatggg ggatccttcc cagatcaaat
    16861 gcgaacaggg tcatcatgcg gcctccgcga cgggatcgcc gccgggccgg ggtgaagcag
    16921 gggatggggc cgtcgcgcca tccagccggt ggtcaaaacg ggttgcgaag cggaataccc
    16981 gctcggggca gacatcgacg caggcgccgc acagcgtgca atctcccgac aggatgaccg
    17041 gcgaggcgcc ggtcttgtcc ccgcgaagcg cagggccaat cacctgcggc tccggacaga
    17101 cagcaaagca gtccatgcaa tcgtcgcaac ggctgcgcgc cgccgccgac acccgaagca
    17161 cggcggcctt gccgatcacg ccatagcagg caccgaccgg acagatatgg ccgcaccagc
    17221 catgtttggc ggtcaggagg tcgaacagga aaatggcggc gaccggcagc aggccgaacc
    17281 atagcccgaa caccagcgcc cgatgtactg cggtcaccgg attgaccagt tcccagacga
    17341 cgatgccggt agcgaacgag gcgccaagca ccgctgcaat catccagttg cgcaaggacg
    17401 gccgcagctt ggcggtttgc gtcaacccaa gccggcgcct gagccaggca gcaagatcag
    17461 ccaccggatt gaccgggcag acccacgaac agaaggttcg gccgccaaag gcaagatagc
    17521 ccccgaccac cagagctgcg ccgatcaccg ccgtcagctc cggccagtgc cgcgccgcca
    17581 gcgtttgcag caataccagc ggatcggtca gcggcaggac gcccagcgtc atcgacgagg
    17641 ccagcgtgcc cttggcgatc caaacgccga acagcggccc ggtgaggaac agcgccagga
    17701 atgcaaactg ggacaggcgc cgcacaatgc cgaagcgatg cgcctgccac catcccttgg
    17761 cggaccgcgc ttcaacgtag gggatcaggt tcgcgctcat ttcggcgtcc cctgcggcgg
    17821 tgcatctggg cgccgaatct ccagcttgac cggagcatcg accagcgcct cgccggcctt
    17881 ctgcttctcc acccagccac gccggtaatg cgcatcctgc gccgcgactg cgatctcgac
    17941 cggcaatacc ttgatcgccg cctcggtgag aacgcaggat ttctcgcatt tgccgcagcc
    18001 ggtgcagaat tcggcatgaa ccaccggttc gaagatcgcg tgtttgccgg tgcggttgtt
    18061 atgcgtccgc tccagcgtga tggccttgtc gatgaccggg cagacccggt agcagacatc
    18121 gcagcgcaag ccctgcagat tgaggcaggt ctcgcggctg gtgatgactg ccacgcccat
    18181 cttggatttg ttgatatcga tgaggctgtg gtcaagcgcc ccggtcgggc aggcttttac
    18241 gcacggaatc gtatcgcaca tctcgcaggg cacattgcgc gcatggaaga acggcgttcc
    18301 gagcgccggc ccgtcggccc agtcggccag cttgagcgtg ccgtaggggc aatcgcgcac
    18361 gcacaacccg cagcgcacgc aggccgagag gaactgcttt tccggcaagg cccccggagg
    18421 ccgcaaggcc tccgccggct tggcgcgcga ctgttcggca aaggccgaga gcatgacccc
    18481 gcaggccagc gcaccgccgc cgaaccgcgc catatcctcc agcgccttgc ggcgctgggg
    18541 gttggcggga gccttggggg tcttgccggc ggcggacatg gcgatgcttc caggtcctta
    18601 tgcggtcacg accttgcagg cgcacttctt gaagtcggtc tccttggaga tcggacaggt
    18661 cgcatccagc gtcagcttgt tggccagctg gctctcgtcg aagaacggga agtagaccag
    18721 ccccttcggc ggcttgttgc gccccttggt ttcgacgcgg gtgatgagtt cgccgcgctt
    18781 ggtctggatc ttcaccgtct ggccgcggtt gagcttgcgc tcggccgcat cgtccggatg
    18841 catgtagagc accgccgacg gcaccgagcg gtgcagttcc ggcacccggc gcgtcatcga
    18901 gcctgaatgc cagtgctcca gcacgcggcc ggtgcacagc cacaggtcga actccttgtc
    18961 cgggatctcc gccgccggct cgaacgggca gaagatggcg cgcgccttct tgtccggctt
    19021 gccgtagaag gagaagccct cgccggcctt cacataggga tcatagccct ccttgaagcg
    19081 ccacagcgtc tccttgttgt cgaccaccgg ccagcgcaga ccgcgcgtct gatggtagac
    19141 atcgaaggag gcaagatcat gcgccttgcc gcggccgaat tcggcatatt cctcgaagat
    19201 gcccttctgg acgtagtagc cgaagtgtgt tgactcgtcg ttgtcatagc ccttctgcgt
    19261 ctcggtcagc gggaacttgt tgatccggcc gttggcgaac agcacatcgt agagcgtctt
    19321 gccgcggtat tcgggcttct tggccagcaa ttcctccggc cacacctctt ccatcttgaa
    19381 gtgcttggca aattccatgg tctgccacag gtcggaccgc gcctcgcccg gtgccttgac
    19441 ctgctgacgc cagaactgcg tccggcgctc ggcgttgcca taggcgcctt ccttctccat
    19501 ccacatcgcg gtgggcagga tcaggtcggc cgccatcgcg gtgacggtcg gatagggatc
    19561 ggagaccacg atgaagttgg ccgggttgcg atagcccgga tagccctcct cgttcatgtt
    19621 cggcgcggtc tgcatgttgt tggtgcactg cacccagtag gcattgagct tgccgtcctt
    19681 cagcatgcgg tgctggacga cggcatggaa gccgatgttg ccgggcaatg tgccttccgg
    19741 aagctgccag atcttttcgg tcaccgcgcg atgctgcgga ttgaccacca ccatgtcggc
    19801 cggcaggcgg tgggcgaaag tgccgacctc gcgcgccgtg ccgcaggccg aaggctggcc
    19861 ggtgagcgag aacggaccgt tgcccggctc cgagatcttt ccggtcagca gatgcacgtt
    19921 gtagatcatg ttgttgaccc acgtgccgcg cgtgtgctgg ttgaagccca tcgtccagta
    19981 ggagacgatc ttcttcttcg ggtcggcgta gagcttggcc agccgctcca gcttgtcctt
    20041 cggcacgccg gacttctcat gcgcgtagtc gagcgtgtag ggctcgagca gcttggcaaa
    20101 ttcctcgaag ccgatcttgt ccttcatgcc ggccggatcc agcgcgccat ccttgccggt
    20161 cttgtgcgag gcgttgaccg ccttggcttc cagcggatgg gtcggacgca ggccatagcc
    20221 gatgtcctcg accgtgcggg cgaattcgac gtgcttttcg gtgaacgcct tgttcaccgc
    20281 gccgttcttg atgatgtagt tctggatgta gttgaggatc gccagatcgc cgtgcggggt
    20341 gaagatcatg gcgttgtcgg ccagttcata cgagcgatgc tcgaaggtcg acaggatatg
    20401 gacttcgcaa ccctcatgag tgaggcgacg gtcggtgatg cgcgaccaca ggatcggatg
    20461 catttccgcc atgttcgagc cccacagcac gaaggcgtcg gcattctcga cgtcgtcgta
    20521 gcagcccatc ggctcgtcga tgccgaaggt gcgcatgaag cctgccaccg ccgaagccat
    20581 gcagtgccgc gcattgggat cgaggttgtt ggaacggaac ccggccttgt agagcttggc
    20641 ggccgcatag ccctcccaga tcgtccactg gcccgagccg aacatgccca cgcccgacgg
    20701 gccggacgcc ttcagcgccg ccttccactt ctcggccatg atcttgaagg cctcgtccca
    20761 ggagatcggc gtgaaatcgc cgttcttgtc gaactggccg ttcttcatgc gcagcagcgg
    20821 cttggtcagc cggtcatcgc catacatgat cttggacagg aaatagccct tgatgcagtt
    20881 gaggccgcgg ttgaccgggg catcgggatc gccctgtgtg gccaccacgc ggccgccctt
    20941 ggtgccgacg atgacgccgc agccggtgcc gcagaaccgg cacacgccct tgtcccagcg
    21001 gatgccgtcg gtcttgccgg cctgcgccag cgcctcttcg gccggcagcg tcacaccgac
    21061 actggcggcg gcggcagccg cagcggtcgc cttgagaacg gcgcgacgat cgtgggcgat
    21121 ctggtcttcg atcggcttga catgtttggt catggttcag gccctttcag agaaacggga
    21181 cgaggttcgg tcagaactgg aacaggtgga acaaccggca ctggagccgt cctccaccgg
    21241 atgggaatga tcgatgccgt gataggccag catggtgctg accacgcccg gcatgcggtt
    21301 gatggcggca agctggtcga tcgccatccc ggaatcggta tcgagaatgg tgatgaccag
    21361 ccttccatcc tcggcctcgg catggacatc caccccgggc aggccgatga ggttctcgcg
    21421 gacgcttgac gccttgccgg gcgccgtctg gacgacacag ccaacaacgt tgagagcatc
    21481 tgactgggaa gcgattgtca ttggcccgcc tccagcgcga caagcccggc gacaaacgtc
    21541 gcacgatcgg cattcaccag ttcaagcccg ttcgtcgggc agacggaaag gcaggctccg
    21601 cagccggtac acaacgcctg atcgaccaga acgtcagcct tgccgcgccc actaagccgg
    21661 aaccggatcg cgccggcctc gcattcatca ccacagcggc ggcaggtgac gcccttcggc
    21721 tcgacgcagg cggcggtcac gcgcgcaagc tgtgctggcg gagcgctgac cgcagcatga
    21781 tcccgaccct caatcggccg gaaggctcgg aataagccac gtctggaaat agccgttgtc
    21841 attcgtctta cctgatcccc ccgcttgttg ttgcatcgcg gcaaggtcgc cttgattttt
    21901 gtcaggttgg ccaaccaagt gaattgagcg tttgaggcat gtcaagcgcc tctgccaatc
    21961 cggcagggat ttttgcctaa ttcttgcgca gatgcgaaaa ggcgccggtt gcgcatgcaa
    22021 ccggtgaaag ctgcgaactg gtgatccgtc gggctctgta aaacgccgtc tcaggcgctc
    22081 gccgcgacca cgctcggagc gggcggcagc tcgttgagtg ggtcgccgat gaaatcgccc
    22141 tgcaaatagg caacacccca atcggccagc agtttggccg attcctgatc ctgcacccat
    22201 tcggccaccg tgcggatatt cagatggctg gccaattgca gcagggtgcg gacaaagaag
    22261 cgatcatcgc tggattgcat gaggttcttg atgaaggtgc cgtcgatctt gagaatatcg
    22321 atcggcagca tccgcatgtt gcggaatgag gtatggccag caccgaaatc gtcgatggcg
    22381 acgcaggcgc cgagggcctt gatcgattcg atcaccttgg tggcaaccga cagatcctcg
    22441 atcagcgatg tctcggtaat ctcgacgatc atccgcggcg caacatcggg atgggcaccg
    22501 atcaggctct tgaacgtgtg catccattcc ggatgcagca cggtgctgac gcccgcattg
    22561 aaggtcagcg tcacatcagc gcgctcctgc agggtggcga acgccagttc catgacccgg
    22621 tggtcgacca gatggacaaa gccaagcttt tcggcgaccg ggatgatctc cccggccccg
    22681 acgagctgac ccgtgcggct gcgcatccgg acaagcgatt catacatggc aatgccgccg
    22741 cccttggcat gggtgatcgg ctggaaggcc agttgaatac gccgctcgtt gagggcggac
    22801 agcacctcgt cggtctgtgc ggcattctgc ttgcggttgg ccgtgcggtg ggcgtccttg
    22861 gtccagatcg caacgacgca attggcatcg gccttggcat cggccagcgc ctcttcggcg
    22921 gcggtcagga tttcggcgac atcgttgaca tcccgaggag cggccaccgt gccggccttg
    22981 accgaggcgg aaatggcgcc cgcgctggtg tcgatcggcc ggtcacgcac aacctgggcg
    23041 atgcgcgaga cggtccgttc gagatcgtcc ggaatggttg ttgccagcag caatgccagc
    23101 ttgttgccgg catagcgacc aatgacgtcg tgcttacgca catgcgagcg gatacggcgg
    23161 gcgatggcag cgatcagctc gtcgccgaca ttatagccat aggccttgtt atgatgggct
    23221 agatcctcga tgccgaagat gaccagtgca agatggcgct tgtccttgcg caccttttcg
    23281 agcgcttcgc caaggcagtt gcagaactcc ggccgggtca gggccccggt agcagggtca
    23341 atctgccgcg tcgtggcaat cgccgcctcg gcgtccgaag cggcggagac cacgcgcacc
    23401 accccatggg ccagccaggg acggccggcc gcattggcga accagcgccc cttgtcctcg
    23461 atccagatgc gccggcccgg cgtcggattg aacagatagc gtgccgaata ggccacgccc
    23521 gtccccttgt cctgacgctg gcattcgaag atcgtcgcct gcaacgaggt cgggctggcc
    23581 gaatcggtga caaccgcaaa tcccgagccc gaggtgatct gcgcaatatc gttgatgccg
    23641 agcacctcgg tcgcgttctg cgaccaggtc atgcggtctg tttcaatgtc ccaggaatag
    23701 acagcttcgc cgactgacga gagaatcgtg cgcggatcga aagcattgga cggcgacgcc
    23761 agcgctggtg aggattgttg ttcagtttga tccacgaaaa cccccaacac cctgcggtcc
    23821 agatgatcca tgccaaaggc tccggacacc tccggaacag ccatggactc tgcttgaaaa
    23881 tggtaaaccg ccagctaacg gcgcggcgat gcgctgcttt cggaaatgcc gcaggggaac
    23941 ggtcaaggcc ccgcgccatg ctggctcgat ccttgcgtga gggcgcccat gcgcccgctc
    24001 cttgtgccag acaggaacag ccacaacggc ccctcccccg gtcctgccgg gccggacgca
    24061 tgctttctcg tccagcttat ccgcgacctg tcgccgtcca tgcgcatcat gccggcgggg
    24121 agccatggca gcgccaacga caaaaagggc gcgtcaaaga cacgcccttc aagcctggac
    24181 ctcagactgt gatccggctc aggcgccggc ttcaaccttc ggcttgagga tctggcgacc
    24241 gcgatacatg cccgacttga ggtcgacatg atgcggacgg cgaagctcgc ccgaattctt
    24301 gtcctcaaca taggccggag cggcaagcgc atcatgcgaa cggcggtggc cgcgcttcat
    24361 gcgggaagtt tttcttttcg gaacggccat cgaactctcc ggcgaacccg cgacggcgac
    24421 ctccggttga cccggtgacg cgtcacgaca aaaagggtga cactatgctc gcatacgacc
    24481 ccaatgcgac aggtgatgct caatcgcatc acaccgccgc cggatcatgc gtgcggaagc
    24541 gcgccttata gtggcctcgt ccgaaaaagg ctagagccaa tttgacacga aatgaaatcg
    24601 gcacgccgtc ttacggaaac ctcgggaatc aagcctttga cgcatccctc gtactcgaca
    24661 gaaccgaccc acaaggcagg agaagcggcc ctggccgatt ggtttgccag cggcgccgcg
    24721 cgctttgaga ccgatctggc cgccctgctc cattccggca accgccggcc cgagcggctg
    24781 atggcggcca tgcgccatgg cacgcttgcc ggcggaaaac gtctgaggcc ctatcttgtc
    24841 ctcaacagtg cgcgcctgtg cgggcagcct gaaggctggg cccgcatcat cgccatggcg
    24901 ctggaatgca tccattgcta ctcgctggtc catgatgacc tgcccgccat ggacaatgat
    24961 gacatgcgcc gtggtcggcc aactgttcac cgggccttcg acgaggcgac agcgatcctt
    25021 gccggcgacg ggttgctgac catggccttc tcgctgctgg cctcggccga tgcgccgggc
    25081 gaccctgcga cccgcctcca catcatcgcc gccctggctg atgccgccgg catcgatgga
    25141 atggtgggtg ggcagatgct cgatcttgcc gccgagggcc gttttgccga tttggctgag
    25201 cccctcgatc tggccgcggt cagtcgtctg caagcgctca agaccggagc actgatccgc
    25261 tttgccgcca tggccggggc gctgatgacc ggcggacgtc agccggatca tctggcggct
    25321 ctcgaccgct acggcacggc gctggggctc gccttccaga tccgcgatga cctcattgat
    25381 gtcgaagtgg attcggcaac ggccggcaag gccaccggaa aggacgacga cgccggaaag
    25441 ggcacatttg tctcgctgct cgggctcgac ggagcccgcg cccggctcga agccgtgacc
    25501 cacgaggggc tttcggcgct cgcccccttc ggggcggcag caggtccgct ggctgcagcc
    25561 ctcaccttca accagacaag gcgtgcctga ccacttcgcg cttggcactg gctcttcgat
    25621 gccgtcctga taggataagc cgaactggga aggatttcag gcgtgaaaaa gcttctgggc
    25681 ctgttgcttg ctggcgttgc cgtggccggc acggtctggg cctttctcgg ccatgataac
    25741 ggcacgccgg ccaccgccag ctaccgcacc gcttccgccg agcgcggaac catcatcgca
    25801 caggtcacca cgtccggcgc cattgccccc gtaacgacgg tgattgtcgg ctcgcaactg
    25861 tcggggcagg tgatcgagat tctggctgac tacaattccg aggtgaagaa cggtcaggtg
    25921 ctggcccggc tcaacagcga tcagatacgt gccaagcttg acggcgcccg cgccgatctt
    25981 gcccaggccc gggccagccg cgtgatgctg gatgcccagc ttgagaaatc cagggcggat
    26041 gcgcagaagg cggccgccaa cgccgacgat atgggccacc agctcgaaaa gaccgaaacc
    26101 cagctcgccg atgcggaaaa gacgctcgcc cggcagacca gcctcaacgc ccagggcatc
    26161 gttgcaacag ccgccctgca gaccgcccag acccagcgcg acaccttgcg tgcggcccgc
    26221 gaatccgccc atgcccagat cgcctccgcc aaggcgcaga ttgccgctct ggccgccgat
    26281 cagaaaatga tcgctgccca gatggtgtcg gccgaggcgc agatcgcgca gcgatctgcc
    26341 gtggttcgcc agatcgaggt tgacctgggc aataccgaca tccgctcgcc ggtcgatggc
    26401 gtcgtcgtgc agcgcaacgt cgaactgggc cagccggtcg ccgcctccct ccaggcaccg
    26461 accttgtttc tcgtcgccca ggacctgcgg caggttcaga tccaggccaa tgtcgatgag
    26521 gccgatgtgg ggcgggtgcg tgatggccag ccggtgagct ttaccgtcaa tgcctatccg
    26581 gggcgcacct ttcagggcaa ggtcaagctg gtccgcctgg gctcgcagac cgtgcagaat
    26641 gtggtgatct acaccacggt catcgatgtc gacaatcccg acatcgccct caagccgggc
    26701 atgaccgcca atctgcgcat tgtgaccgaa cagcgcgaca atgtcgtgcg cgtgccgaac
    26761 gcggcactgc gctgg
//
LOCUS       DAOMXG010000011        25413 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_1227_length_25413_cov_10.021965, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000011 DAOMXG010000000
VERSION     DAOMXG010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 25413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25413
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1227_length_25413_cov_10.021965"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /locus_tag="PLQ11_01570"
     CDS             <1..872
                     /locus_tag="PLQ11_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23622.1"
                     /translation="APASGTWRFFSNEDGIVALFEKAGAEADSPALLRLTCVSEDATL
                     NILSRGVLDDEVEQRAERNELVLTLTTGGRTLSLPGYIARAPEATVAGFETPLTPALM
                     DMLKSPGLSLETAGVSLKVSDAGMRRALDQFIAACPAPAAAADASGWGSYTGLSHGYR
                     LELPKSLFRLQAGDRNGRHYTAERGLMELRIYGEANAMERKFPAVVQELAPDIDKVTL
                     RRTGKDFLVLSGHAGSRIVYFLGRATCNNSNVAFINLTYDPEAIDFYAPIITRMAQSF
                     LKETWPDGKPLCP"
     gene            869..4054
                     /locus_tag="PLQ11_01575"
     CDS             869..4054
                     /locus_tag="PLQ11_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23623.1"
                     /translation="MMRRRAAHFFLLLSAMLAGVVLAGTSAAAQSLTQAADGLMAAFE
                     NRLPPEARQMVVRPFGPSGARLVENQGATLWRVSYGRALSSRILHDYFSAGVRVEFDA
                     RRYRTREAAVADCQRNPRWPTGSAEQALQVPARLARYECARAFAKHDVERQKLPHNMR
                     PYEWRTRVHVFSRGPYVVEVSEHAISDDVDHTPAPQFPAQPLIDEIADRLDRLTDAGS
                     AQKPATGSGQSARPPDPAQPGSKLTKQSRDLVSLLKTPEGGLLLSALGASALSALGAL
                     LAMTQSGVPATQALNDLGNLLRGRLPPDAYADWRARQQALGNRIVRSGRVEYVQPMSQ
                     MVEDLDPGGLSPFRSGQISAAPPTPPLQPRDGETNPATGEVWSDEDRGWVSRSMYDWE
                     QKNRAEIKRIAEYNRTAVMSWQREAQELSQKIAEAKASQQAAAEAEAQRRALLASKLS
                     KAYAAEGKDPLEIAGLLASGNSAELQDRYASHLRSVMNRSSAEAAAEARWATAMDVGY
                     YTSKIVLAGAKTGLMAVGGPMSAAVSIGANAVISGVEEGTTAYLDPSGKKSVAGSFAA
                     GFVSGVKDGAIGRYTNLPGTSTIKRVLLPAAGDAGEMYLRTGDVEKSLTTGLLSASGG
                     VISHGMSDIGHAGLRGGTEVLAGGIIGGAQSQIAGGSFAEGFADGLASSIGSKAGGAI
                     ATAGAPMTAQDLAMDAGYRANRARAEDLAGELDHALRSGSDADKAKAVNAVLSHREAR
                     QFLKTIPDETLKKGYADLADAHRTKPLIDDTVNRLNKVTMTAPDGTTVQRYGVMTDSG
                     KGHVLVPIDPAHFRSGSGTQGGAPGMDLDLYVKAKIIDKATGRPLKPDIVDDALGEAC
                     ASLGISRKLQEINYVHPKHAEAFALQPGEDPQAFIARGGTLTGREAQSVTEVAGYKLN
                     EAKLLYQPGQAHTADGGASSLSELSRIAIKDHDRLTTKLLETHPDAQVPAVFSQIDGT
                     TGRTPLDIMREVGNGTLSPGIGNAQFRALTGMSLESGATKLVSLPEFIGKGMGGGGQA
                     NTVYVPGTASTDITGAALREAMRSTLNRSGR"
     gene            4056..5084
                     /locus_tag="PLQ11_01580"
     CDS             4056..5084
                     /locus_tag="PLQ11_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRK23624.1"
                     /translation="MTDPAPYPDHLTLSEAAFAAALSGQEPAGADAQARPILTSLAAS
                     RVTLNLRLWAGPGASSETSMMFPDAIGLGGGVVANRLATGEVAVSGFVDGETPARLVA
                     AMLPPDRPVPVFALQLAPPVLMALAALLDIGVSEALTRRIAGAEERRPVAAGVIADMP
                     AIGAGAVAGWLASQWGLGGFGQLTTFVLALGLVTAPPDRAGVAQMLDALARQGLLTAE
                     KSGRYRPTELLEPLASLRLGLTAGLQWQRLTRLDETSVEVSEKLFLLGPEGCMLDLSA
                     TAAGLIRMEIVGRRQVVDFIARDCVAGPPRPADTPAAGGPMKRFCGACGARLNPGARF
                     CGTCGKAL"
     gene            5142..6161
                     /locus_tag="PLQ11_01585"
     CDS             5142..6161
                     /locus_tag="PLQ11_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015342664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HRK23625.1"
                     /translation="MTMKALRIEAEGIARFADVPRRAPGAGDVRVRVRHVGLCGSDLN
                     TFRGLNPLVQLPRIPGHEIGGVIVEAGAGVGPEYRPGRHVIVLPYTTCGTCSSCRKGR
                     FNACRYNRTLGVQQDGGLAEEIVLATDKLILNDTLEPRFLALVEPLSVGFHAVARGEV
                     GSGDRVVVLGCGMIGMGIVIAAAARGAEVIAVDPSDDKTALALSFGARHALNPERDDI
                     AAGVAALTGGDGADVAFEAVGLPATFTQAIDLACFAGRIVYVGYAKAPVSYQTQMFNL
                     KELNIMGSRNATRADFEAVIAHLATIGSAADRLISRIIPFAEAESALPYWSQNRDVVK
                     IIIEV"
     gene            6267..7688
                     /locus_tag="PLQ11_01590"
     CDS             6267..7688
                     /locus_tag="PLQ11_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HRK23626.1"
                     /translation="MAGGTFFDEMGGFDGSVRDGYKVLAKWLEQAPPDLLANRKAEAE
                     HFFRRIGITFNVYGDDQGTERLIPFDLVPRILTSKEWQRLSRGLEQRVNALNAFIGDV
                     YGEQRTIREGIVPEDLVMTNPCFRPEMTRFRPARDIYVQIAGIDIVRVDADTFYVLED
                     NARTPSGVSYMLENREAMLRLFPELFATMRIAPVDNYADALLSTLKSVAPVSAPADPT
                     VVLLTPGQFNSAYYEHSFLADKMGIELVEGRDLFVRANVVYMRTTEGPRRVDVIYRRI
                     DDEYLDPLAFNPTSMLGVPGLMSAYRAGNVTISNAVGTGVADDKAIYSYMPEIVKFFT
                     DEEPILNNVPTWRCREPDALKYVLEHLDELVVKEVHGSGGYGMLVGPHATKAERQAFA
                     AKIAASPDGFIAQPTLSLSTVPCFVESGLAPRHVDLRPYVLCGADGVRTVPGGLTRVA
                     LKQGSLVVNSSQGGGTKDTWVVE"
     gene            7693..8634
                     /locus_tag="PLQ11_01595"
     CDS             7693..8634
                     /locus_tag="PLQ11_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018263153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HRK23627.1"
                     /translation="MLSRTADNMFWLSRYIERAENLARILDVASRMATLPSAYAGSSN
                     EWESAIATAACQDAFHSFYDKATRDNVVEFIVASPDNPSSIKSCIDIARSNARAVRTA
                     MTGEMWEIINDAWHEMQKINLRKLDAARLPRFLTFVKEASLRFDGAAYRTMLRTDHYW
                     FQRLGTFVERADNTARLLDVKYHVLLPETEAIGGGLDYFQWSSILRAVSAQTSFHWVY
                     RESVKPWLVADFLILSRKQPRSLISTCIQINDVLDRLSETYGRRGTSQRLARQIYAIL
                     DGAEAEALFQGGLHEFLADFIARNNELGQAISQQYLQ"
     gene            8637..9461
                     /locus_tag="PLQ11_01600"
     CDS             8637..9461
                     /locus_tag="PLQ11_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019905188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HRK23628.1"
                     /translation="MRLTVRHDTLYRYARPAVSAIQHLRMTPRGHDGQFIRKWRVEID
                     ADCRLDREEDAYGNIVHTFTADGPISELRVSVAGDVETIDTHGVVAGTIERFPLVFWQ
                     RATALTEACRNIAEFARDTSSGEGGDALATLHALNAGLVRLMRFAVGDTDTRTTASDA
                     FAARAGVCQDFAHVFIAAARSLAIPARYVGGYYLRTDMNEQEAGHAWAEAHVAGIGWI
                     GFDPAHGMSTTDRYVRVAAGLDYLDAAPIRGTRIGGSGETMAVTVHVEQGPVIVEE"
     gene            9603..10343
                     /locus_tag="PLQ11_01605"
     CDS             9603..10343
                     /locus_tag="PLQ11_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HRK23629.1"
                     /translation="MTYGVGVLVKEGLVMVADTRTNAGLDNIATFRKLHLFEDPGEKT
                     MALATAGNLAISQSVISLLREGIRNPETDRIETLTDQPSLFKAAQFVGHVIRTVHDTD
                     GVSLERNGVSFEVSMLLGGQFKGHSPRLFMLYKAGNFIEATPDTPYFQVGEHKYGKPI
                     LDRAVTHKSSLLDCLKLSLISMDSTMRSNLSVGLPIDVVMLREGAEGWDINRRIEAGE
                     PYFHDLRERWSAALRKAHTDIPTPPYRD"
     gene            10727..10803
                     /locus_tag="PLQ11_01610"
     tRNA            10727..10803
                     /locus_tag="PLQ11_01610"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10761..10763,aa:Arg,seq:acg)
     gene            complement(11402..12319)
                     /locus_tag="PLQ11_01615"
     CDS             complement(11402..12319)
                     /locus_tag="PLQ11_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23630.1"
                     /translation="MALGGDGAFPILSISGEQGTGKSTFATLLRKLVDPNEVDHRSLP
                     RDTRDLAVAACNSHVLSFDNLSHIDAEMADALCRASTRSGFATRQLHSDKEETVIQLH
                     NPVILNGIPALTERADLADRLVTVRLLPFPESERRTERELWPAFESEAPFILGALLDG
                     LCSGLRNIEKVELPTKPRMADFVTWVEACAPGLGWAQGDFAALYTDNRANAADSAFEA
                     DAVAVAIATMMQARGRVTWTGTPTELLAELALHTSEAARHARTWPVTAQGLGNRMDRA
                     APLLRRRGVHFEKRHSGQRYYSIWCDPAE"
     gene            complement(12326..13057)
                     /locus_tag="PLQ11_01620"
     CDS             complement(12326..13057)
                     /locus_tag="PLQ11_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23631.1"
                     /translation="MTVPDDQDSGTAPANDPHQRISDMVEGSFNNPLPAFSHGVEDTG
                     RADGPPDPNDRTQRKRRKRPASSKVGESDASARQRAPAQRDNLLSVCQTVDLWHAPDG
                     TEYATFSVRAHRENWPIRSRRFKSWLAGRAYAELGIAPGAQALEDSLRILESRALHEG
                     TERVPIKRTARRDGLAIIDTGRADWSVIEVAGSGWRIVERHDWPIVRSNSAQALPVPE
                     AGEAIDRLRQFVNTSEEADFKLAVA"
     gene            complement(13054..13683)
                     /locus_tag="PLQ11_01625"
     CDS             complement(13054..13683)
                     /locus_tag="PLQ11_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23632.1"
                     /translation="MSRPIAGADFDAVFVPHGGNGGGGDDDTKHGTLQYWCPYCECVH
                     VHGAANRGDEPRIEHRGAHCFHGVSPHAGRGIDLAVRPSSEASPLYPKALIDTAHHAL
                     TPRLRANLHAQGPAIRRAFHKALLLDTGAHRRKGKWGVWHGPDLRAFAIEGAGVTPAM
                     ANDLADLAARIFGVPWEVVARRFLETAIGRPLPAERALAVEAALRGDDA"
     gene            complement(13680..13895)
                     /locus_tag="PLQ11_01630"
     CDS             complement(13680..13895)
                     /locus_tag="PLQ11_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRK23633.1"
                     /translation="MTQSKLVDEKQAAEFLALSPRTLQAWRLTGGGPAFKKIGRVVRY
                     DSADLATFISARTHTSTASVTMKEAAQ"
     gene            complement(14134..14424)
                     /locus_tag="PLQ11_01635"
     CDS             complement(14134..14424)
                     /locus_tag="PLQ11_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23634.1"
                     /translation="MAPEIRVNGTLTASSRGWFNIDSEMHIRQIEKLAGLCVMVCDMV
                     QIQDDPTENAASLASKLEAVFIEIEARAEWLREIAEIESYPTFKQAQAEQAT"
     gene            complement(14471..14758)
                     /locus_tag="PLQ11_01640"
     CDS             complement(14471..14758)
                     /locus_tag="PLQ11_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23635.1"
                     /translation="MAPTFDNASLDMLADEIGALDLEIKALEGRMAAAKAELKARKVE
                     KAEGRRFAVTVADSLRSSLDTKGIREAMGEAWCNLHSKIAVVTTVRIKAIA"
     gene            14843..15097
                     /locus_tag="PLQ11_01645"
     CDS             14843..15097
                     /locus_tag="PLQ11_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRK23636.1"
                     /translation="MTVEQIKAARALLRWNQADLAEASGVSLPTIRRLEAQTGPLTGY
                     SRTQDAIRDAIETAGVEFIEAGSSSAPAGAGVRLKGKCDE"
     gene            15090..16259
                     /locus_tag="PLQ11_01650"
     CDS             15090..16259
                     /locus_tag="PLQ11_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23637.1"
                     /translation="MNERPLLADACKRIVLPETWQAWCDATDPENAEETPLPGASSAE
                     LVRHQYHIRQTVLALAEAADALNRDLRGVFTAGGWLAWGFRFCRTDPLRQNPSLSLIG
                     ASEWGRFDVINVPGSRLESKHSGAGYEGICCAPLLSRANASNAEAVLAGISLSGAVER
                     FILADPEVQNLYRPSRRMQGMEGFADAFTKAITAGVTLPLSDKPTIASALLPIITPLV
                     NGNAEALADVLARRFDALAFYLDKLHVAWGNDPDVPAGKLDADFVRATTRLNLSDFSL
                     ADAGAGEAITNLRFAPPSKANAPSASDSKSKGGRPRIYDKHLAALEVEVVADPQKAKR
                     LTTKTAIYCAVEKRISGGDSAAGTKGGKGAEKAFAGNPLAHSIMERIISVAKNLS"
     gene            complement(16231..16440)
                     /locus_tag="PLQ11_01655"
     CDS             complement(16231..16440)
                     /locus_tag="PLQ11_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23638.1"
                     /translation="MPVEVKPWDVTEILTSEEIIAAYLEEAQAEGDPAMLAKALEHVQ
                     RAREKNREASSAEEAGVKTGSSQLK"
     gene            complement(16451..16780)
                     /locus_tag="PLQ11_01660"
     CDS             complement(16451..16780)
                     /locus_tag="PLQ11_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23639.1"
                     /translation="MGLRSIKDHPFYIRMKLNAPEEVATWVLGEVPIEEAGAKDRKAA
                     FRRVAKDITSGKAERTKYGFNSDTNGEIARAMEWAFKAGMKAGQTATAKPKKSAGKPS
                     APSSCND"
     gene            complement(16799..17089)
                     /locus_tag="PLQ11_01665"
     CDS             complement(16799..17089)
                     /locus_tag="PLQ11_01665"
                     /inference="COORDINATES: protein motif:HMM:NF027249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HRK23640.1"
                     /translation="MLAYFVCILEGDGDNWGARIFDIDGCVGAGSTPDEAIAGVTEAL
                     RDVIDHMRNDNHPVPSPSNIAHVLASGEVRAGEHLVLVPLWGGTEDHALAGA"
     gene            complement(17103..17294)
                     /locus_tag="PLQ11_01670"
     CDS             complement(17103..17294)
                     /locus_tag="PLQ11_01670"
                     /inference="COORDINATES: protein motif:HMM:NF025742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HRK23641.1"
                     /translation="MSTPEWVAKFDKAEVRRGRPKAEAPKVSTTLRLDADIIERFRAD
                     GPGWQSRINAALRDWLKAG"
     gene            complement(17326..17541)
                     /locus_tag="PLQ11_01675"
     CDS             complement(17326..17541)
                     /locus_tag="PLQ11_01675"
                     /inference="COORDINATES: protein motif:HMM:NF016268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HRK23642.1"
                     /translation="MARAGEVFESPHVTIADDRKDYGEDRFLSVGFLDARMVFLAWTP
                     RGAARRIISMRKANGREQEKYGHRLIQ"
     gene            complement(17671..18936)
                     /locus_tag="PLQ11_01680"
     CDS             complement(17671..18936)
                     /locus_tag="PLQ11_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019962497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRK23643.1"
                     /translation="MDEAKPAEKPAFLWDSEVPGFGALVLASGVKSFIFQYRNEARRS
                     RRLTIGRYGAMTVDEARALAREAAVKVSKGGDPVSARRAYREAPTMAQLLDRYLAEHV
                     SNHNAESTAKDIAALIEKHIRPRIGSIKANDFKRSDAAKLHTAMKQTPRRANYALAIL
                     SKALSLAEVWDIRPENTNPCGSIQRYQENHRTRFLSAEEVTRLGEALVEAETIGLPWR
                     IEGGKANSKHLPKDDKRRTPLSWQAIAAVRLLLLTGARLNEIISLKWADVDATAGTIA
                     LPGKKGGKREPHPVSAAVLTILASLPRPKGASHVLPRDSGPKRHISREVMEAAWQRLR
                     WCVGIEDVRIHDLRHTVGTYAAQSGVSSFIVRDLLRHQNITTTARYANFDAKPVRQIS
                     NTLGDMLTANLEGQTGAQVVPFGRGVKAT"
     gene            19428..19688
                     /locus_tag="PLQ11_01685"
     CDS             19428..19688
                     /locus_tag="PLQ11_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23644.1"
                     /translation="MRVVAQTHLVRRGNTFHFRIAVPLELVARVGKVEIKTTLRTTDP
                     LTARQRGRVLSSAIEALFEEVRRMPELTRQHVERRVREYFQA"
     gene            complement(19754..20023)
                     /locus_tag="PLQ11_01690"
     CDS             complement(19754..20023)
                     /locus_tag="PLQ11_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23645.1"
                     /translation="MFDSSWGRQATAPQLLPRACADRPSEPGRVSETFAALKANPVTA
                     NIRNKVKFILAIDGEMVEAEVLTSDEPSLAWNTPHFDGIIKMRIT"
     gene            complement(20108..20181)
                     /locus_tag="PLQ11_01695"
     tRNA            complement(20108..20181)
                     /locus_tag="PLQ11_01695"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20147..20149),aa:Gly,seq:tcc)
     gene            20354..21508
                     /locus_tag="PLQ11_01700"
     CDS             20354..21508
                     /locus_tag="PLQ11_01700"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRK23646.1"
                     /translation="MQKIDDILAAAVGAGDLPCIVAMVADRHGVVYAGAHGRQNMAEA
                     GPARLDTTFRLHSMTKAITAVAALQLVERGLIDLDEPLGARFPALADKHILTGFAADG
                     TPLTRKAQRPITLRHLLTHTAGFCYDMWNGDIARWQKATGTPGSRSGKLASLNQVLSF
                     EPGTRWQYSIAIDHVGRIVEALTGTDLETCLRRSVLDPLGMTETTFRQSQTQAERRVA
                     MHRRLADGGIEALPSPPEVPEYLNGGGALFGTVPDYLKFTRCLLAGGAPLVRPDSCAA
                     MVSNQLGSLQIETLKAAIPALVADVEFWPGLAKGWGLSFLINLERTPEGRNAGSFGWA
                     GLSNLYYWVDPAAGLTGVFASQMLPFFDRRAIDAFRAFERAVYEEFGKTA"
     gene            21600..22127
                     /locus_tag="PLQ11_01705"
     CDS             21600..22127
                     /locus_tag="PLQ11_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23647.1"
                     /translation="MPTDSAPTGFVYAAIAQNEVERGYVKIGYCADPPSRYASRTGAP
                     YRRMLLIQPDRLLPAKDHVAQGAIEQQVLDFFRRGRGMLYEGRAFPPGYAELVRFDEG
                     AREALDRRLRRPNASMASPEAMRFIRTLLAMWEDQMPVDPWSAQAVLPDLAMVPPQRR
                     PAPRINTAAEPQLWM"
     gene            complement(22307..22391)
                     /locus_tag="PLQ11_01710"
     tRNA            complement(22307..22391)
                     /locus_tag="PLQ11_01710"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22355..22357),aa:Tyr,seq:gta)
     gene            22531..23337
                     /locus_tag="PLQ11_01715"
     CDS             22531..23337
                     /locus_tag="PLQ11_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRK23648.1"
                     /translation="MRKDRPHRPPPHRKDRNAGRPRPSGAGDLDGLHLIYGLHSVREA
                     LANPGRTFRSLLVTENALARLTEGGQALPIQPEIVRPDVIGRQLTPDAVHQGMLLIAE
                     PPPAPPLDALPRGSLIIALDQVTDPHNVGAILRSACAFGVSAVITPVRHSPEVTGVLA
                     KAASGALEHVPIIAVRNLVRTLEELGERGLLRVGLDSDAPVDLGEAQLALPMVLVLGA
                     EGKGLRQSTRAACDILARIDLPGAIRSLNVSNAAAVSLYAIRRRLDRPRG"
     gene            23667..25346
                     /locus_tag="PLQ11_01720"
     CDS             23667..25346
                     /locus_tag="PLQ11_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007764382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRK23649.1"
                     /translation="MAHAPTIDDRTRPMTAEEKKVILASSLGTVFEWYDFYLYGSLAA
                     MIATHFFSAFDINTRSIFALLAFAAGFLVRPFGALVFGRLGDLIGRKQTFLITILIMG
                     LSTFFVGLLPSYATIGWIAPVTLIALRMLQGLALGGEYGGAAVYVAEHAPEGRRGFYT
                     SWIQTTATLGLLLSLFVIVGLRLWMGEQEFAAWGWRIPFLGSIFLLAISVWIRLQMQE
                     SPAFQKMKEEGSLSKAPLSEAFGQWKNLKIVILALVGLVIGQAVVWYTGQFYALFFLQ
                     AILKIDLLTANVLIAWSLILGTGGFIVFGSLSDRIGRKPVILGGCLIAALTFFPLFKM
                     MAETANPKYAKALDAVKVEVSADPATCGSLFDPVGIRQFSAPCDLARRALSQQAIKYS
                     YVPGAAGAPLKVTVNGQEAAWGAGLADFTKNIGAAATAAGYPAANDADKVKVSGFFAA
                     LGDAKALKIIGILTILVIYVTMVYGPMAAALVEFFPTRIRYTAMSLPYHIGNGWFGGL
                     LPATSFAMVAATGDIYYGLWYPVGFAIMTFIVGLLFVKETHKRDLRTFEGN"
BASE COUNT         4541 a         8092 c         7874 g         4906 t
ORIGIN      
        1 cggcgccagc cagtggcaca tggcgcttct tctccaacga ggatggcatt gtcgcgctgt
       61 tcgaaaaggc cggcgccgaa gccgacagcc ccgccctgct gcggctgacc tgcgtgagcg
      121 aggatgcgac cctcaacatc ctgtcacgcg gcgttctcga tgacgaggtc gagcaacggg
      181 ccgaacgcaa cgagctggtc ctgaccctga ctaccggggg ccgcacgctg agccttccgg
      241 gctacattgc ccgggccccg gaagcgacgg tggcggggtt tgaaacgccg ctgacgccgg
      301 cattgatgga tatgttgaaa agccccggct taagccttga aacagcaggt gtttcattga
      361 aggttagcga tgccggcatg cgccgggctc tggatcagtt catcgccgcc tgcccggcgc
      421 ctgccgccgc ggcggatgcc agcgggtggg gcagctatac cggcctcagc catggctacc
      481 ggctggaact gccaaaaagc ctgttccggc tgcaggccgg agatcgcaac gggcgccact
      541 acaccgccga acgcggactg atggagctca ggatctacgg cgaggccaat gcgatggagc
      601 gcaaattccc cgccgtcgtg caggagcttg cccccgatat cgacaaggtg acgctgcgcc
      661 gcaccggcaa ggactttctg gtgctctccg gccatgccgg cagccgcatc gtctattttc
      721 tcggccgggc gacctgcaac aacagcaatg tcgccttcat caacctgacc tacgaccctg
      781 aggcgatcga cttctacgcg ccgatcatca cgcgcatggc gcagagcttc ctgaaggaga
      841 catggccgga cggaaagccg ctctgcccat gatgcgccgc cgagccgccc atttcttcct
      901 gctgctgagc gcaatgctgg caggtgtcgt gctggccggc acaagcgctg cggcgcagtc
      961 tttgacgcaa gctgccgatg ggttgatggc ggccttcgag aaccgcctgc cgcccgaagc
     1021 gcggcagatg gtggtgcggc ctttcggccc gtccggcgcc cgtctggttg aaaaccaggg
     1081 cgcaacgctc tggcgcgtat cgtatgggcg ggcgctgtcc tcgcgcatcc tgcatgacta
     1141 tttctctgcc ggagtccggg tcgagttcga tgcccggcga taccgcacgc gcgaggccgc
     1201 cgttgccgat tgccagcgca acccgcgctg gccgaccggt tcggccgaac aggccttgca
     1261 ggtgccggcg cggctggcac gctatgaatg tgccagggct ttcgccaaac atgacgtaga
     1321 acggcagaag ctcccccaca atatgcgacc ctatgaatgg cggacgcgcg tgcatgtgtt
     1381 cagccggggt ccctatgtcg tagaggtttc cgaacacgcc atcagcgacg atgtcgacca
     1441 tacccctgcg ccgcaatttc cggcccagcc gctgattgac gagatcgccg accggctcga
     1501 ccgtctgacg gatgcgggct cggcgcagaa gccggccacc ggcagcgggc agagcgcgcg
     1561 gccgcccgat ccggcccagc cgggcagcaa gctgaccaag caatcgcgcg atctcgtgag
     1621 cctgctcaag acgccggaag ggggattgct gctctctgcc cttggcgctt ccgccctgtc
     1681 ggcgctcggc gcgctgctgg cgatgacgca atcgggcgtt ccggcgacgc aggcgctcaa
     1741 tgacctcggc aacctgctgc gcggccgtct gccgccggat gcctatgcgg actggcgcgc
     1801 ccgccagcag gcgctcggca accggatcgt gcgcagcggg cgggtcgaat atgtccagcc
     1861 gatgtcgcag atggtcgagg atctcgatcc gggcggcctc agcccattcc gcagcgggca
     1921 gatcagcgcc gctcctccga caccgccgct gcagccgcgc gacggcgaga ccaaccccgc
     1981 caccggcgag gtgtggagcg atgaggatcg cggctgggtc agccgttcaa tgtatgactg
     2041 ggaacagaag aaccgcgccg agatcaagcg catcgccgaa tacaaccgca cggcggtgat
     2101 gagctggcag cgcgaggcgc aggaactcag ccagaagatc gccgaggcca aggccagcca
     2161 gcaggccgcc gccgaggccg aggcacagcg ccgcgccttg ctggcgagca agctcagcaa
     2221 ggcctatgcc gccgagggca aggatccgct ggaaatcgcc gggctgctgg ccagcggcaa
     2281 cagtgcggag cttcaggatc gctatgccag ccacctgcgc tcggtgatga accgcagctc
     2341 tgccgaagcg gcagccgagg cgcgttgggc cacagcgatg gatgtcggct attacacatc
     2401 caagatcgtg ctggccgggg ccaagaccgg attgatggct gtcggcgggc cgatgagcgc
     2461 ggccgtctcc atcggcgcca atgcggtcat ctcgggcgtc gaggaaggca ccaccgccta
     2521 tctcgatcca tccgggaaga agagcgtggc ggggtccttt gccgccggct tcgtctccgg
     2581 ggtcaaggat ggtgcgatcg gccgttacac caacctgccc ggcacatcga cgatcaagcg
     2641 cgtgctgctg ccggcagcag gggatgccgg cgaaatgtat ctgcggaccg gcgatgtgga
     2701 aaaatcgctg accacagggc tgctctcggc ttccggcggc gtgatcagcc atggcatgtc
     2761 cgacatcggc catgccggcc tgcgcggcgg aaccgaggtt ctggccggcg gcattatcgg
     2821 tggagcgcaa tcgcagattg ccggaggcag ctttgccgaa ggcttcgccg atggtctggc
     2881 aagcagtatc ggcagcaagg cgggcggtgc catcgcgacc gccggcgccc ccatgaccgc
     2941 ccaggatctg gcgatggatg ccggctaccg cgccaaccgc gcacgcgccg aggacctggc
     3001 tggagaactc gaccatgctc tgcgttccgg ctccgatgca gacaaggcca aggcggtcaa
     3061 tgccgtgctg tcgcatcgcg aggcgcggca gtttctcaag accatccccg atgagacgct
     3121 gaaaaaaggc tacgccgacc ttgccgatgc gcatcgcacc aaaccgctga tcgacgatac
     3181 ggtgaaccgc ctcaacaagg tgaccatgac agcgccggat gggacgaccg tgcagcgcta
     3241 tggcgtgatg accgacagcg gcaaggggca tgtgctggtg ccgattgatc cggcccattt
     3301 caggagcggc agcggcacgc aaggcggcgc tcccggcatg gatctcgatc tttatgtgaa
     3361 agcaaagatc atcgacaagg cgaccggccg gccgctcaag cccgacatcg tcgatgacgc
     3421 gctgggtgaa gcctgcgcct cgctcggcat cagccgcaag cttcaggaaa tcaactacgt
     3481 tcaccccaaa cacgccgagg ccttcgcatt gcagccaggc gaggatccgc aggcattcat
     3541 cgcccggggc ggcacactga ccgggcgtga ggcgcaatcg gtgacggaag tggccggcta
     3601 caagctcaac gaggccaagc tgctctacca gcccggccag gcccatacag ccgacggcgg
     3661 ggcctcctcg ctttcggaac tgagccgcat cgcgatcaag gatcatgacc ggctgactac
     3721 caagctgctc gaaacacatc ccgatgccca ggttccggcg gtgttcagcc agatcgacgg
     3781 cacaacgggc cgcaccccgc tcgatatcat gcgcgaagtg gggaacggca cgctcagccc
     3841 aggcatcggc aatgcgcagt ttcgggcgct gaccggcatg tcgcttgaat ccggcgccac
     3901 caaactggtc tcgctgccgg aattcatcgg caagggaatg ggcggcgggg ggcaggccaa
     3961 cacggtctat gtgcccggca cggcctccac cgacatcacc ggcgcggcgc tgcgggaagc
     4021 gatgcgctcg acgctcaacc gaagcggacg ctgacatgac cgatccagcg ccctaccccg
     4081 atcatctgac cctcagcgag gcggcgttcg ccgcagccct gtccggacag gagccggccg
     4141 gagccgatgc gcaggcccgg cccatcctga ccagcctggc ggccagccgc gtcacgctca
     4201 atctccggct ctgggccggt cccggcgcga gcagcgaaac cagcatgatg tttcccgatg
     4261 ccatcgggct tggcggcggt gtggtggcaa accggctggc gaccggcgaa gttgcagttt
     4321 caggctttgt tgacggggaa acgccggcgc ggctggtggc ggcgatgctg ccgcccgatc
     4381 ggcccgttcc ggtgtttgcg ctgcaactgg ctccgcctgt cctgatggcg ctggccgcgt
     4441 tgctggatat cggcgtcagc gaagccctga cccggcgtat cgccggcgca gaggagcggc
     4501 gccccgttgc ggccggggtg atcgccgaca tgcccgccat cggcgctggc gccgtcgccg
     4561 gctggcttgc atcgcaatgg ggtcttggcg gcttcgggca gctgaccacc ttcgttctgg
     4621 cgctcggact ggtgactgcg ccgccggacc gtgccggggt tgcgcagatg ctggacgcat
     4681 tggcccggca gggactgctg accgccgaaa agtccgggcg atatcggccg accgagttgc
     4741 tcgagccgct ggccagcctg cggctgggac tgaccgctgg cctgcaatgg cagcggctga
     4801 cccggcttga tgagaccagt gtcgaggtca gcgaaaagct gttcctgctt ggccccgagg
     4861 gatgcatgct cgacctctcc gccacggcgg cggggctgat ccgcatggag atcgtcgggc
     4921 ggcggcaggt tgtcgacttc atagcccgcg attgtgtcgc cgggccgcct cgccctgcgg
     4981 acactcccgc tgccggtggg ccgatgaaac gcttctgcgg cgcctgcggc gcccgtctca
     5041 atcccggggc ccggttttgc ggcacctgcg gaaaggcttt atgaccggtt tctcgtccgg
     5101 cgccggggcc ggagccggca actcaggaga gcaggcagcc catgaccatg aaggctctca
     5161 ggatcgaagc cgaaggcatt gcccgtttcg ccgatgtgcc ccgccgtgca ccgggggcgg
     5221 gcgacgtgcg cgtccgcgtg cgccatgtcg gcctgtgcgg cagcgacctc aacaccttcc
     5281 gcggcctcaa cccgctggtg cagctgccgc gcataccggg gcacgaaatc ggcggagtga
     5341 ttgtcgaggc cggcgccggt gtcggaccgg agtatcggcc cggccgccac gtcatcgtgc
     5401 tgccctacac cacctgcggc acctgttcct cctgccgcaa gggccggttc aacgcctgcc
     5461 gatacaatcg cacgctcggg gtgcagcagg atggcgggct ggccgaggag atcgttctgg
     5521 caaccgacaa gctgatcctc aacgatacgc tggagccacg gtttctggcg ctggtcgagc
     5581 cgctttcggt gggcttccac gcggtggcgc gcggcgaggt cggcagcggc gaccgcgttg
     5641 tggtgctcgg ctgcggcatg atcgggatgg ggatcgtgat tgcagcagcg gcgcgcgggg
     5701 ccgaggtgat cgcggtcgat cccagcgatg acaagaccgc gctggcgctg agtttcgggg
     5761 ctcgccatgc gctgaacccc gaacgggatg acattgccgc cggtgtcgcc gccctgaccg
     5821 ggggggatgg cgccgatgtc gccttcgagg cggttggcct tccggcgacc ttcacccagg
     5881 ccatcgacct tgcctgcttt gccgggcgca tcgtctatgt cggctatgcc aaggctccgg
     5941 tgagctacca gacgcagatg ttcaacctca aggagctgaa catcatgggc tcgcgcaatg
     6001 ccacccgcgc cgatttcgag gccgtcatcg cccatctggc gacgatcggt tccgccgcgg
     6061 atcgcttgat ctcccggatc attccctttg cagaagcgga gagcgccctg ccctactgga
     6121 gccagaaccg cgacgtggtg aagatcatca tcgaagtcta gatcacgctg gccgccaatc
     6181 cagcagttcc gcatccggct ttggaacagg gcttgcttgc gggaccaaaa agggattgcc
     6241 tgaggcaggg gcaggaaagg catgtaatgg cgggcgggac attcttcgac gagatgggcg
     6301 gcttcgatgg cagcgtacgc gacggctaca aggtgctggc aaagtggctt gagcaggcgc
     6361 cgcccgacct tctggcgaac cgcaaggcgg aggccgagca ttttttccgc cgcatcggca
     6421 tcactttcaa cgtctacggc gacgatcagg gcacagaacg gctgattccg ttcgatctgg
     6481 tgccgcgcat tctcacctcg aaggaatggc agcggctgtc gcgcgggctg gagcagcgcg
     6541 tcaatgccct caacgccttc atcggcgatg tctacggcga gcagcgcacc atccgcgaag
     6601 gcatcgtgcc ggaggatctg gtgatgacga atccctgctt ccggccggag atgacgcggt
     6661 ttcgaccagc gcgcgacatt tatgtgcaga ttgccggaat tgacatcgtc agggtcgatg
     6721 ccgacacgtt ctacgtgctt gaggacaatg cgcgcacgcc ctccggcgtt tcctatatgc
     6781 tggagaaccg cgaagcgatg ctgcggctgt ttccggaact gttcgccacg atgcgcatcg
     6841 ctccggtgga caattacgcc gatgcgctgc tgtcgaccct gaaatcggtg gctccggtct
     6901 cggcgccggc cgatccgacg gtggtgctgc tgacccccgg gcaattcaat tccgcctatt
     6961 acgaacactc cttccttgcc gacaagatgg ggatcgaact ggtcgaggga cgcgacctgt
     7021 tcgtgcgcgc caatgtcgtc tacatgcgca ccaccgaagg tccgcgccgc gtcgatgtca
     7081 tctatcggcg catcgacgat gaatatctcg atccgctcgc cttcaacccg acctcgatgc
     7141 tcggcgtgcc cgggctgatg agcgcctacc gcgccggcaa tgtcaccatt tccaacgcgg
     7201 tcggcaccgg tgtcgccgat gacaaggcga tctattccta catgccggag atcgtaaaat
     7261 tcttcaccga tgaggagccg atcctcaaca atgtgccgac ctggcgctgc cgcgagccgg
     7321 atgcgctcaa gtatgtgctc gaacatctgg acgaactggt ggtgaaagaa gtgcacggct
     7381 cgggcggcta cggcatgctg gtcggtccgc atgcgacaaa ggccgaacgg caggctttcg
     7441 ccgccaagat cgccgcaagc ccggacggct tcattgccca gccgacgctg tcgctctcca
     7501 cggtgccctg ctttgtcgaa tccggcctgg cgccacgcca cgttgatctg cgcccctatg
     7561 tgctctgcgg cgccgatggc gtgcgcacgg tgccgggcgg cctgacccgc gtggcgctca
     7621 agcagggctc gctggtcgtc aattcctcgc aagggggcgg aaccaaagac acctgggtgg
     7681 tggagtagga ccatgctgag ccgcaccgcc gacaacatgt tctggctgtc gcgctatatc
     7741 gagcgcgccg agaacctcgc ccgcattctc gacgttgcct cgcgcatggc aacgcttccg
     7801 agcgcctatg ccggctcgtc caacgaatgg gaaagcgcca tcgccaccgc tgcctgccag
     7861 gatgcgttcc attccttcta tgacaaggcg acgcgcgaca acgttgtcga gttcattgtc
     7921 gcctcgccgg ataacccgtc ctcgatcaag tcctgcatcg acattgcccg ttccaatgcc
     7981 cgcgccgtcc gcacggccat gaccggcgag atgtgggaga tcatcaacga cgcctggcac
     8041 gagatgcaga agatcaatct gcgcaagctc gatgcggcgc ggctgccgcg ctttctcacc
     8101 ttcgtcaagg aagcctcgct gcgctttgat ggcgccgcct accgcaccat gctgcgcacc
     8161 gatcactact ggttccagcg cctcggcacc ttcgtcgaac gcgccgacaa caccgcccga
     8221 ctgctcgacg tgaaatacca tgtgctgctg ccggagaccg aggcgatcgg cggcgggctc
     8281 gactatttcc agtggtcgtc gatcctgcgc gcggtttcgg cgcagacctc gttccactgg
     8341 gtctaccgcg aatcggtcaa accctggctg gtggccgatt tcctgatcct cagccgcaag
     8401 cagccgcgct cgctgatctc gacctgcatc cagatcaatg acgtgctcga ccggctgtcc
     8461 gagacctatg gccggcgcgg caccagccag cgcctcgccc ggcagatcta cgcaatcctc
     8521 gacggggccg aggccgaggc gctgtttcag ggaggattgc atgagttcct ggccgatttc
     8581 atcgcccgca acaacgagct tggccaggcc atctcccagc agtatctgca atagccatgc
     8641 gcctgaccgt ccgccacgac actctctatc gctatgcacg gccggctgtc tcggctatcc
     8701 agcacctcag gatgacgccg cgcgggcacg acggccagtt catccgcaaa tggcgcgtcg
     8761 agatcgatgc cgattgccgg ctcgaccggg aggaggacgc ctacggcaac atcgtgcata
     8821 cgttcaccgc cgacggtccg atcagcgaat tgcgcgtctc ggtggccggc gatgtcgaaa
     8881 ccatcgatac ccacggggtg gtcgccggaa cgatcgagcg attcccgctg gtgttctggc
     8941 agcgggccac agccttgacc gaggcatgcc gaaacattgc cgaatttgcc cgcgacacct
     9001 cctccggtga aggcggcgat gcgctggcaa cccttcacgc gctcaatgcc gggctggtgc
     9061 ggctgatgcg ctttgcggtt ggcgataccg atacgcgcac cacggccagc gatgcattcg
     9121 ccgcaagggc cggcgtctgt caggattttg cccatgtgtt catcgcagcc gcgcgcagcc
     9181 tggcgatccc cgcgcgctat gtcgggggct attacctgcg caccgacatg aacgagcagg
     9241 aagccggcca cgcctgggcg gaggcccatg tcgccggaat cggctggatc ggcttcgacc
     9301 cggctcacgg catgtccacc accgaccgct acgtgcgcgt tgccgccggg ctcgattatc
     9361 tcgatgcggc cccgatccgc ggcacgcgca tcggcggatc gggcgagacc atggctgtca
     9421 ccgtccatgt cgaacagggg ccggtgatcg tcgaggagta gagcattttc aggcgaagtg
     9481 gagtccggtt cgcgtcagga aaatgcgatc attcgagaaa ccatccacaa ttgccaagcc
     9541 ggcgggccat cgctatcagg cgggcggcga tgctttgaga attgaacccg gggactgatg
     9601 caatgacgta tggcgtgggc gttctggtga aggaaggtct ggtcatggtg gccgacaccc
     9661 gcaccaacgc gggtctcgac aatatcgcta ccttccgcaa gctgcacctg ttcgaggatc
     9721 cgggcgagaa aaccatggcg ctggccaccg ccggcaatct cgccatcagc cagtcggtga
     9781 tctcgctgct gcgcgaaggc atccgcaacc cggagaccga ccgcatcgag accctgaccg
     9841 accagccgag cctgttcaag gcggcccagt tcgtcggcca tgtcatccgc acggtgcacg
     9901 ataccgacgg cgtttcgctg gagcgcaacg gcgtctcctt cgaggtctcg atgctgctcg
     9961 gcggccagtt caagggccat tcgccgcggc tgttcatgct ctacaaggcc ggcaatttca
    10021 tcgaggcaac gcccgatacc ccctatttcc aggtcggcga gcacaagtac ggcaagccca
    10081 tcctcgatcg cgccgtgacc cataaatcca gcctgctcga ctgcctcaag ctcagcctca
    10141 tctcgatgga ttcgaccatg cgctcgaacc tgtcggtcgg cctgccgatc gacgtggtga
    10201 tgctgcgcga gggagccgag ggctgggaca tcaaccgtcg catcgaggcg ggcgagccgt
    10261 atttccatga ccttagggaa cgctggtcgg cggcgctgcg caaggcgcat acggacattc
    10321 ccaccccgcc ctaccgggac tgacccgagc cgcgcatgtc gatgttgccg ccaggattct
    10381 gcgcttcaac gcaaacgtcc ggccagccct gacagcccca gaaacgccgg cctgccgttg
    10441 ccagtggcag aatgggtggg cagatcatgc tcctgttttg aaagaaacat caatgcgaga
    10501 gataacatga tcgttatcaa gtgcttagag catgcttgtt gcgattagcc ggattccaac
    10561 cgatcgcaac atgctctcgt tgtcgcaagc ccggcacggt ggccaaaagc tcctgggcca
    10621 ggcggccaag tggccagcca ctgtccggcc gatttaacgc aaaacgctct tgcaatacgg
    10681 acgcgatagg gctaatcaac cgcctcgctt ggatcaccaa gcggccgcgc ccgtagctca
    10741 gctggataga gcatcagact acgaatctga gggtcagagg ttcgaatcct ttcgggcgcg
    10801 ccattttatc tcattgatat tattgagata atcaaccaac ggtcactgtt ggctctttcg
    10861 gctgcgtttt ccgtgtaagc aatatgtaag caccagcggc aatgtcgccg cccattcgcc
    10921 tgcttccggc ctgcccctgc gcccgctgct tcagcctgct tccttccgtt ccccgcgccc
    10981 cttgccaacc ttggggctac cggcgtccat catgagtgac accgctatct cttttctcca
    11041 aaagagagaa agccgtccaa gccgtccaca ttgtccaaac gacatgaatt caatggcttg
    11101 taggccaggc caagccgtcc acgggtcgtc caaggtcgtc caccccatcg acataggggg
    11161 tggggggcat tgggtccttc ctgcaccccc taccccctgc gggcgggacg cggctaccca
    11221 gattcgctag cactgcaagt ccaattctgg gttgacacta ctgacacacc tgacaccgtg
    11281 ttgacgcacc tgacaccctg acacgttttg gttgtggcac tcgcggcgca tcagaaaaag
    11341 tgaaagcgcc ggaagggggc ggacctccgc gctcggttgc atcatggggt ttggggagtg
    11401 gttattctgc gggatcgcac cagatcgagt aatagcgctg gcctgaatgg cgcttctcga
    11461 aatgcacgcc gcgacgcctg agcaagggcg cggcacggtc catccggttg ccaagccctt
    11521 gggcggtgac aggccatgtg cgggcatggc gcgccgcctc actggtgtga agcgccagtt
    11581 ccgccagtaa ctcggttggc gtgccggtcc atgtgacgcg cccgcgcgcc tgcatcatcg
    11641 tggcgatggc cacggccacc gcgtctgcct cgaaggcgct atctgcggca ttggcgcggt
    11701 tgtcggtgta gagcgcggcg aaatcgcctt gcgcccatcc gaggccgggg gcgcacgctt
    11761 ccacccaagt cacaaagtcc gccatgcgag gctttgttgg caattccact ttttcgatgt
    11821 tcctgaggcc ggaacatagc ccgtccaaca gcgcaccaag aatgaagggc gcttcgctct
    11881 cgaaagcagg ccaaagttcg cgctcggttc gccgctcgct ttctggaaac ggaagaagcc
    11941 gaacagtcac cagccggtca gccaaatcgg cgcgctcggt caaagccggg atgccgttga
    12001 ggatcacggg gttatgaagc tggataaccg tttcttcctt gtcggaatga agctgccgcg
    12061 tggcaaaacc ggatcgggtt gaggcgcggc aaagcgcatc ggccatttca gcgtcgatat
    12121 gagacaggtt gtcgaatgac aggacgtgcg aattgcaggc cgcgaccgcc aaatctcgcg
    12181 tgtcgcgggg gagcgagcgg tggtccactt cgttcggatc caccagtttc cgcaacaagg
    12241 tggcaaaggt ggattttccc gtgccctgtt cgccggaaat cgacaggatc ggaaaggcgc
    12301 cgtccccgcc caatgccatc acaagtcagg ctacggccag cttgaaatct gcttcctctg
    12361 atgtgttcac gaactgccgt agtcggtcga tagcctcccc cgcctctggc acgggcaatg
    12421 cctgagccga attgctgcgc acgattggcc aatcgtgacg ctccacgatg cgccaaccgc
    12481 ttcccgccac ctcgatcacg ctccaatcgg cgcgccctgt gtcgatgatg gccagaccat
    12541 cacgccgcgc tgtccgcttg atcggcacgc gctctgtgcc ttcatgcaag gcgcgcgatt
    12601 caaggatgcg caaggaatcc tcaagcgcct gcgcaccggg cgcaatgcca agttctgcat
    12661 aggctcgtcc ggccagccat gacttgaagc gccgcgacct gattggccag ttctcacgat
    12721 gcgccctcac tgaaaacgtg gcgtattccg tgccatcggg cgcgtgccac aagtcgaccg
    12781 tctggcaaac gctcaacagg ttatcgcgtt gggctggtgc gcgctggcgg gctgatgcgt
    12841 cgctttctcc cactttcgag gaagccgggc gcttgcgccg cttcctttgc gtgcggtcgt
    12901 tgggatcggg cggcccatct gcccgccccg tatcttctac cccatgcgag aatgccggta
    12961 gagggttgtt gaatgaaccc tccaccatgt ctgaaatgcg ctggtgtgga tcgttcgcgg
    13021 gcgcagtccc ggagtcttgg tcatccggca ctgtcatgcg tcatccccac gcaatgccgc
    13081 ttccaccgcc aacgcgcgtt cggccggaag cggcctgccg atagcggttt ccagaaaccg
    13141 ccgggcgaca acttcccacg gcacgccgaa gattcgcgcc gcaaggtcag ccaagtcatt
    13201 tgccattgct ggtgtgacgc ccgcgccctc gatggcgaat gcccgcaagt ccgggccatg
    13261 ccacacgccc catttgccct tccgcctgtg cgcgcccgtg tccagcaaca gcgccttgtg
    13321 aaaggcccgc cggatcgcag ggccttgcgc gtgaaggttg gcgcgcaggc gcggcgtcaa
    13381 ggcgtgatgc gccgtatcga taagcgcttt cgggtagagt ggcgatgcct cgcttgaggg
    13441 cctgacggca aggtcaatcc cgcgcccggc atgggggctt accccgtgaa agcagtgtgc
    13501 cccgcgatgc tcgatgcggg gttcgtcgcc gcgattggca gcgccatgga catgcacgca
    13561 ctcacaatag gggcaccagt attgcaatgt gccgtgcttg gtatcgtcgt cacctccgcc
    13621 gccattgccg ccatggggca cgaaaacggc gtcgaagtcg gctccggcta tcgggcgact
    13681 cattgtgcgg cctccttcat ggtgacgctg gcggtgctgg tgtgcgtccg cgcgctgatg
    13741 aaggtggcaa gatcggcgct gtcatagcgc acaacgcggc cgatcttctt gaaagcgggg
    13801 ccgccgccgg tgagccgcca tgcttgcagg gtgcgaggcg atagcgccag aaactccgcc
    13861 gcctgctttt cgtccaccaa cttcgattgg gtcatcgaag gctttccttc cgttacgctc
    13921 acggaccatc cgagagcgtg gaaagcgtct gacgactggc ggcctttgtc ttccctctaa
    13981 acccctgaaa tacccctgaa acccggttaa tggggtgcta cttggcccgc ccattgtgat
    14041 gtttggccca atccagtgcc catcgggcga cggcctgagc cgcgcgggcg attgtcatga
    14101 aggccgccgc cattgcggca tggaatatgc gcttcacgtc gcctgttccg cttgggcctg
    14161 cttgaaagtc ggatagcttt cgatttccgc gatctctcgc aaccattcgg cccgcgcctc
    14221 gatctcgata aacaccgcct ccagcttgga tgccagcgat gccgcgttct cggtggggtc
    14281 gtcctgaatc tgcaccatgt cgcagaccat tacgcatagc ccggccaact tctcgatttg
    14341 ccggatgtgc atttccgaat cgatgttgaa ccagccccgg cttgaggcgg tcagggttcc
    14401 attcactctg atttcggggg ccatcttcgt tcctttctgg tttctggtgg tgtgccgcat
    14461 cgcctgaggt tcaggcgatg gccttgatgc gaacggttgt gacgacagca atcttgctat
    14521 gcagattgca ccaggcttcg cccatcgcct cccgaatgcc tttcgtgtcc aacgaagaac
    14581 gaaggctatc cgccactgtc acggcgaagc ggcgaccttc cgccttctcc accttgcggg
    14641 ccttcaactc cgccttggcg gcggccatgc ggccttccag cgctttgatc tccagatcca
    14701 atgcgccgat ttcgtcggcc agcatatcga gcgaagcatt gtcaaaggtg ggcgccattt
    14761 gggtttctcc ttgcatattg atatttttaa tataatctat atgaagcagt gtcaatatta
    14821 aaaatatcat ctttggtgcg atatgacagt cgaacaaatc aaggcggcgc gcgcccttct
    14881 ccggtggaac caagccgact tggcagaggc ttccggggtg tcactgccaa ccattcggcg
    14941 cttggaagcg caaacaggcc cattgaccgg ctatagccga acgcaagatg ccatccggga
    15001 tgccattgag acggctggtg ttgaattcat cgaggcggga tcgtcaagcg cgcctgccgg
    15061 tgcgggtgtg cggttgaagg ggaaatgcga tgaatgagcg ccctttactg gccgatgcct
    15121 gcaaaaggat cgtgcttccc gaaacttggc aggcatggtg cgatgccact gatccagaga
    15181 atgcagagga aacgccgctt ccgggcgcaa gctctgctga acttgttcgc caccagtatc
    15241 atatccgcca aactgttttg gctctggcgg aagcagccga tgcactgaac cgtgatttgc
    15301 gcggcgtttt cacagccggt ggttggcttg cttggggatt tcgattttgt cgaacagatc
    15361 ctcttcgcca gaatccgtcc ttgtcgttga ttggtgccag cgaatggggt cggttcgatg
    15421 tgatcaatgt tcccggttcg aggttggaat ctaagcactc cggcgcgggt tatgaaggca
    15481 tatgctgtgc cccgctcctg tcgcgggcca acgcaagtaa tgccgaagca gtgcttgccg
    15541 gtatctctct ttcaggcgct gttgagcgct tcattctggc ggacccggaa gtgcagaacc
    15601 tctatcgccc atcgcgccgg atgcagggga tggaaggatt tgccgatgcg ttcacgaaag
    15661 ccatcacggc gggggtgacg ttgccgctat ccgacaagcc gaccatcgcg tcggcattac
    15721 tgccgatcat tacgccgctg gtgaatggaa atgccgaagc ccttgccgat gtgctggcgc
    15781 gccggtttga tgcgctggcc ttctatctgg ataagttgca tgtggcctgg ggcaatgatc
    15841 ctgatgttcc ggcgggtaag ttggacgctg attttgtgcg cgcaacaacg aggctcaact
    15901 tgtccgattt cagcctagcc gacgcaggtg caggggaagc tatcaccaat ctccgtttcg
    15961 cgccgccttc aaaggccaat gccccttcgg caagtgacag caagtcgaag ggtggaaggc
    16021 cacgcatcta cgataaacac ttggccgcgc tggaagttga ggtggttgcc gatccacaaa
    16081 aggcgaagcg cttgaccacc aagacagcaa tttattgtgc ggtcgagaag cgcatttcgg
    16141 ggggcgactc tgccgctgga accaagggtg gcaagggggc agaaaaggct tttgcaggca
    16201 acccattggc tcattcgatc atggaacgca tcatttcagt tgcgaagaac ctgtcttgac
    16261 gccggcttcc tccgccgaac tggcctcccg gtttttctcc cgcgcccgct gcacatgctc
    16321 aagcgccttg gccagcatgg ccggatcgcc ttcggcctgc gcttcttcga gataggccgc
    16381 aatgatctcc tcggaagtca ggatctcggt tacatcccaa ggcttcactt caaccggcat
    16441 ggccttcctc tcaatcgttg catgaacttg gggcggatgg ctttcctgcg gatttctttg
    16501 gcttcgctgt tgccgtctgc ccagccttca tcccggcctt gaaagcccat tccatcgcac
    16561 gcgcaatttc gccgttcgta tccgaattga agccgtattt cgtgcgttct gccttaccgg
    16621 atgtgatgtc cttggccacg cgccggaacg cagccttgcg gtctttcgcg cccgcttctt
    16681 caatgggcac ttctccaagc acccacgtcg caacttcttc tggcgcgttc agcttcatgc
    16741 ggatgtagaa tgggtgatcc ttgatgctgc gcaggcccat ggttttttcc tgtatcgcct
    16801 atgcgccagc gagcgcgtga tcttcggtgc cgccccacag gggaacaagc acaaggtgtt
    16861 cgccagccct cacttcgcca cttgccagaa catgggcgat gtttgagggg cttggaacgg
    16921 ggtgattgtc gttgcgcatg tggtcgatca catcgcgcag cgcctcggtc actccggcaa
    16981 tggcctcatc cggtgtgctg cctgcaccaa cgcatccgtc gatatcgaag atgcgcgcac
    17041 cccaattgtc gccatcgcct tcgaggatgc acacgaaata ggccagcatg gcaccctctc
    17101 catcagcctg ctttcagcca atcccgcaag gcggcattga tgcgggattg ccagcccgga
    17161 ccatcggccc ggaagcgctc gataatgtcg gcgtcaaggc gcagcgtggt ggaaaccttg
    17221 ggggcttctg ccttgggtct gccgcgccgc acctccgcct tgtcgaactt cgcaacccat
    17281 tccggggtgc tcatgtcggg gatttcatca gggtctaccc atccttcatt ggatgaggcg
    17341 atggccatac ttttcttgct cgcgcccatt tgctttcctc attgagatga tccggcgcgc
    17401 agcgccacgc ggcgtccacg ccagaaagac cattctggca tccaagaacc caacagagag
    17461 aaaccgatct tccccgtagt ctttacgatc atccgcgata gtgacgtgcg ggctttcaaa
    17521 aacctctccg gcccgcgcca tgtccacgcc gcgatggtcc agagtggctt gcgcttggcc
    17581 ggatcgaatt cgatttccat gattattgtt actacaaaaa cagcgacgga acaaggattt
    17641 tgttactacg aaaatttgga cacggcgcag ttaggtcgcc ttcacgccgc gcccgaaagg
    17701 cacaacctga gcacctgtct gcccctcaag gttcgctgtc agcatgtcgc ccagggtgtt
    17761 ggaaatctgc cgcaccggct tggcgtcaaa attggcatag cgggcggttg tcgtgatgtt
    17821 ctgatggcgc aacaaatcgc gcacgatgaa actggaaacg ccggattgcg ccgcataggt
    17881 gccaaccgtg tggcgaagat catggatgcg cacatcttcg atgccgacgc accagcgcag
    17941 gcgctgccac gcggcttcca ttacctcgcg tgagatatgg cgctttgggc cactgtctcg
    18001 cggcagaaca tgtgaagcgc ctttcgggcg tggcaggctg gcaaggatcg tcagaacggc
    18061 ggcgctgacc gggtgcggtt cgcgtttacc acccttcttt cccggcaggg cgattgtgcc
    18121 tgccgttgcg tccacatccg cccatttcag gctgatgatt tcattgaggc gcgcgcccgt
    18181 gagcaagagc aagcgcaccg ctgcaatggc ctgccaggat agcggcgtgc ggcgcttgtc
    18241 atccttgggc aggtgcttcg aattggcctt gcccccctcg atccgccaag gcaaaccgat
    18301 ggtttccgcc tccaccagcg cttcgcccag ccgggtcact tcttcggcgc tgagaaaccg
    18361 tgtccggtgg ttttcctgat agcgctggat tgaaccgcac ggattggtgt tctccggtcg
    18421 aatgtcccaa acctccgcaa gcgaaagcgc cttggacagg atcgcaaggg catagttcgc
    18481 ccggcgcggt gtctgcttca tcgccgtgtg caacttggcc gcatcggatc gcttgaagtc
    18541 attggccttg atcgagccaa tccggggcct gatgtgtttc tcgatcagcg ccgcaatatc
    18601 cttggcggtg gattcggcat tgtggttgct gacatgctcg gcaaggtagc ggtccagcaa
    18661 ttgcgccatc gtcggcgctt cccgataggc gcgtcgggct gacacgggat caccgccctt
    18721 gctcaccttg accgccgctt cgcgcgcaag ggcgcgggct tcatcaaccg tcatcgcacc
    18781 atagcgccca atcgtcagac gccgtgagcg ccgggcttca ttccgatact ggaagatgaa
    18841 ggacttgacg ccgctcgcca gcaccagcgc gccaaagccc ggcacttcgc tatcccacag
    18901 gaacgcgggc ttttctgccg gtttggcctc atcgatcagg cgtttgtcag cttgggcatg
    18961 gcgtgtgttc cggttggacg atccttggac ggttttggac gacgaatgga cgacctttgt
    19021 attcagttat ctatttgttt ttattgttat atttctagtt atggacaact tggacggctt
    19081 tccgccccta ctgtcttaaa tgagcactga tgttcatcgc cctgttggcc acctcatccc
    19141 atgtaagcgc catgtaagca atatgtaagc aggaaagcca gaatgagtgg gtattacatc
    19201 gcgttccatc gcacccgtta tgcctgtaac ccattgaaat atcgtaaatc gtagtagtgc
    19261 ttggtaagta gcagaaagtc aacaccacag actacgaatc tgagggtcag aggtttgaat
    19321 cctttcgggc gcgccatctt ctccgcaagg ctctgctctt gctcaggttt tcaggaaact
    19381 agctacgccg ttgcccttct tggccagaac tttggccaga aaggaccatg cgcgtggtcg
    19441 cccagaccca tctcgtcagg cgtgggaaca ccttccactt ccgcatcgcc gtgccgcttg
    19501 aactggtggc gcgtgtcggc aaagtcgaga tcaagacgac gctcagaaca actgacccct
    19561 tgacggcgcg gcagcgcggt cgtgtgctca gcagtgctat agaagccctg ttcgaggagg
    19621 tgagacgaat gcccgaactg acgcgccaac atgtcgagcg acgcgtcagg gagtattttc
    19681 aggcctagag catgtcgcga ttaagtggat gccggtcaat cgcagaagac atgctctaac
    19741 ttattgatac cgatcacgtt atccgcattt tgatgattcc atcaaaatgc ggcgtgttcc
    19801 aggctagcga gggctcgtcc gatgtgagga cttctgcttc caccatctcg ccatcgatgg
    19861 cgagaatgaa cttcaccttg ttgcgaatgt tggcggtgac ggggttggcc ttcagcgccg
    19921 cgaaggtttc gctgacgcgg cccggttcac tgggtctatc ggcgcatgca cgtggcagca
    19981 actgcggagc ggtggcttgc cgcccccacg aagaatcaaa caaccgtcgg acctttggct
    20041 gcgggacccc acagggaccc catactgcgc cagcgaacag caatcttatc taggttattg
    20101 gagccattgg agcgggtgaa gggaatcgaa ccctcgtatt cagcttggaa ggctgctgct
    20161 ctaccattga gctacacccg cgaggccggc gcgaggccga cggttttcta tttggcgtga
    20221 atcgcggcgt ttgccaaggt agggaacgac attaaggctg ctgtcgatgg cagggcacga
    20281 cgcgggccag aagcggccct gctgtttcgg ccaggccagc gccaggacag gcacggacaa
    20341 gcgggagaca ggtatgcaaa agatcgacga cattctggcc gcagccgttg gcgccgggga
    20401 cctgccgtgc atcgtcgcca tggtcgccga ccgtcacggc gtcgtctatg ccggcgcaca
    20461 tggccggcag aacatggcgg aggcggggcc ggcgaggctg gacacgactt tccgcctgca
    20521 ctcgatgacc aaggcgatca ccgccgtcgc cgctttgcaa ctggtcgagc gcgggctgat
    20581 cgacctcgac gaaccgctcg gcgcgcggtt ccctgccctc gccgacaagc acatcctgac
    20641 cggttttgcc gccgatggca ccccgctgac gcgcaaggcg cagcgaccga tcacgctccg
    20701 ccatctgctg acccataccg ccggcttttg ctatgacatg tggaacggcg acatcgcgcg
    20761 ctggcagaag gccacgggaa cgccgggttc gcgctcgggc aagctcgcct cactcaatca
    20821 ggtcctgagc ttcgaaccgg ggacgcgctg gcagtattcc atcgccatcg atcatgtcgg
    20881 ccgcatcgtc gaggcgctga ccggcaccga tcttgaaacc tgtctcaggc ggtcggtgct
    20941 cgatccgctc ggcatgacgg agaccacgtt ccgccagagc cagacgcagg ccgaacggcg
    21001 ggtagccatg caccgccggc tggccgatgg cggcatcgag gcgctgccct ccccgcccga
    21061 ggtgcccgaa tatctcaatg gcggcggcgc gctgttcggc accgtgccgg attatctgaa
    21121 attcacccgc tgtctgctcg ccggcggggc gccacttgtc cgccccgaca gctgcgccgc
    21181 catggtcagc aaccagctgg gctcacttca gatcgaaacg ctcaaggcag cgataccggc
    21241 cctggtggcc gatgtcgaat tctggccagg tctggccaag ggctggggcc tgtcgttcct
    21301 gatcaatctg gaacggacgc ccgaaggccg caacgccggc tcgttcggct gggccggcct
    21361 cagcaatctc tactactggg tggatcccgc agcagggctg accggcgtct ttgccagcca
    21421 gatgctgccg ttcttcgacc ggcgggccat tgacgctttc cgcgcgttcg agcgggcggt
    21481 ctatgaggaa ttcggcaaaa cggcgtgaat acccgccgtt ttgttcaaat tgcagcgatt
    21541 tccgcagcgg ttatgcaggg catggcgggc atgattgccc cgatcaggag accctgccca
    21601 tgccgactga ctctgccccg accggatttg tctatgccgc catcgcccag aacgaggtcg
    21661 agcgcggcta tgtgaagatc ggctattgcg ctgatccgcc ctcccgctac gcgtcgcgaa
    21721 ccggggcgcc ctatcggcgc atgctgctga tccagcccga tcggctgctg ccggcaaagg
    21781 atcatgtcgc gcaaggggcg atcgaacagc aggtgctcga tttcttccgg cgcggacgcg
    21841 gaatgctcta tgagggacgc gccttcccgc ccggctatgc cgaacttgtc cgcttcgatg
    21901 agggtgcgcg cgaggccctc gaccgccggc tcaggcggcc gaacgccagc atggcctcgc
    21961 ccgaggcgat gcgcttcatc cgcaccttgc tggcgatgtg ggaggaccag atgccggtcg
    22021 atccgtggag cgctcaggcg gttctgccgg atctcgccat ggtgccgccg cagcgccgcc
    22081 cggcgccgcg gatcaatacc gcagcagagc cgcaactgtg gatgtagagc attttcaagc
    22141 aaagtggagt ccggttcgcg tcaagaaaat gcgaaaaaca aaaaataaag ccttttcaag
    22201 cgaagtggtg cccggttcgc gtcaagaaaa tgcgaaaaac aaaaatagag ccatttttcg
    22261 tgcactggat ttcgccgaaa gcgcactaac cggtcgcggg aatgaatggt gggagaggta
    22321 ggactcgaac ctacgaaggc ttagccagcg gatttacagt ccgccccctt tgccgctcgg
    22381 gacactctcc cagctcggcc tgaaccgagg cgcgctttat cgtcagggag ccggggcctg
    22441 tcaaccgctt ttgcggccgc gacgtgtttt tgccgggtcg ccctgccagg gcggcttttc
    22501 cttccgcgca tttcctgcca tggaaccccg atgcgcaagg acagacccca ccgcccgccg
    22561 cctcatcgca aggatcgcaa tgccggtcgc ccccggccgt ccggcgccgg agatctcgac
    22621 ggtctgcatc tcatctacgg gctgcattcg gtgcgggagg cattggccaa tccgggccgg
    22681 accttccgat cgctgctggt gaccgaaaat gccctggcgc ggctgacaga gggcgggcag
    22741 gccctgccca tccagccgga gatcgtccgg ccggatgtca tcggacggca gctcacgccc
    22801 gatgccgtgc atcagggcat gctgctgatc gccgaaccgc cccctgcccc gccgcttgac
    22861 gccctgccgc gcggttcgct gatcatcgcg ctcgatcagg tgacggaccc gcacaatgtc
    22921 ggcgccatcc tgcgctcggc ctgtgccttc ggggtcagcg ccgtgatcac gccggtccgc
    22981 cacagccccg aggtgaccgg cgtgctggcc aaggcggcct ccggcgcgct cgaacatgtg
    23041 ccgatcatcg ccgtccgaaa tctggtgcgg acgctggagg aactgggcga gcgcggcctg
    23101 ctgcgtgtcg gccttgattc cgacgcgccg gtcgatcttg gcgaggcaca gcttgccctg
    23161 ccgatggttc tggtgctcgg cgccgagggc aagggactgc gccaatcgac ccgcgcggcc
    23221 tgcgacatcc tcgcccggat cgacctgccg ggcgcgatcc gcagccttaa tgtctcgaac
    23281 gccgccgcgg tcagcctcta tgcaatccgc cggcgactcg accgcccccg gggctgacgg
    23341 aagcgtgtgc agcgcaatat gtgcacttgc gaattctggc gtgaatcgcg gctttttcgc
    23401 cgttttttcc cgggtttcgc gcataaatat gattaattga tacgaaagtc taatagccca
    23461 attggcgagc attggcgcct cgaccgcact ttaacaaaag attaatcctt gaaccaagac
    23521 tgcgcggttt ggcgtccgga ctgttccggg gggtgccggg cgtagtccag ggtcaaatcg
    23581 gctctgaaaa aaaccgttcg ccggtgtgct cgcctgtctg ggccatccgg tggattgatc
    23641 gaaacttggg aaagggaaca gatcccatgg ctcacgcacc gactattgac gatcgcacgc
    23701 gtccgatgac ggcggaggaa aagaaggtca ttcttgcctc ctcgctcggc actgtgttcg
    23761 agtggtatga cttctacctc tacggctcgc tcgcggcgat gatcgcgaca cacttcttct
    23821 cggccttcga tatcaacacc cgctcgatct tcgcgctgct ggcgttcgcg gcgggctttc
    23881 tggtccgccc gttcggcgcg ctggtgttcg gccgcctcgg cgacctgatc ggccgcaagc
    23941 agaccttcct gatcaccatt ctgatcatgg gcctgtcgac cttcttcgtc ggcctgctgc
    24001 cgtcctatgc caccatcggc tggatcgctc cggtcaccct gatcgcgctg cgcatgctgc
    24061 agggcctggc gctcggcggt gagtatggtg gcgcggctgt gtacgttgcc gagcacgctc
    24121 cggaagggcg tcgcggtttc tacacctcgt ggatccagac cacggcgacc ctcggtctgc
    24181 tgctgtcgct gttcgtcatc gtcggcctgc gcctgtggat gggcgagcag gaattcgcgg
    24241 cctggggctg gcgcattccg ttcctcggct cgatcttcct gctggccatc tcggtttgga
    24301 ttcgtctcca gatgcaggag tcgccggcgt tccagaagat gaaggaagag ggttcgctgt
    24361 ccaaggcgcc gctgtcggaa gccttcggcc agtggaagaa cctgaagatc gtcatcctcg
    24421 cgctcgtcgg tctggtcatc ggtcaggcgg tcgtgtggta tacgggtcag ttctatgccc
    24481 tgttcttcct gcaggccatt ctgaagatcg acctcttgac ggccaacgtg ctgatcgcct
    24541 ggtcgctgat cctcggcacg ggcggcttca tcgtcttcgg ctcgctgtcg gacaggatcg
    24601 gccgcaagcc ggtgatcctc ggtggctgcc tgatcgcggc gctgaccttc ttcccgctgt
    24661 tcaagatgat ggcggagacg gccaacccga agtatgccaa ggcgctggac gcggtgaagg
    24721 tcgaggtttc ggctgatccg gccacctgcg gctcgctgtt cgatccggtc ggcatccgtc
    24781 agttctcggc tccgtgcgat ctcgcccgcc gcgccctgtc gcagcaggcg atcaagtaca
    24841 gctacgtgcc gggcgctgcc ggcgctccgc tgaaggtgac cgtgaacggc caggaagctg
    24901 cctggggtgc cggcctcgcc gacttcacca agaacatcgg cgctgcggcg accgctgccg
    24961 gctatccggc cgcgaacgat gctgacaagg tgaaggtgtc gggcttcttt gctgccctgg
    25021 gtgatgccaa ggcactgaag atcatcggca tcctgaccat cctcgtcatc tatgtgacga
    25081 tggtgtatgg cccgatggcg gcggcgctgg tcgagttctt cccgacccga atccgctaca
    25141 ccgcgatgtc gctgccctac cacatcggca acggctggtt cggcggcctg ctcccggcga
    25201 cctcgttcgc catggtggcg gcaaccggcg acatctacta cggcctgtgg tatccggtcg
    25261 gcttcgcgat catgacgttc atcgtcggcc tcctgttcgt gaaggaaacc cacaagcgcg
    25321 acctgcgcac cttcgagggc aactgatcgt tgcgtcgggc gcctcggcgc ccggcactcc
    25381 tcgcaagaac aggaagggcc cgccccggcg ggg
//
LOCUS       DAOMXG010000012        24568 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_1269_length_24568_cov_11.133847, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000012 DAOMXG010000000
VERSION     DAOMXG010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 24568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24568
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1269_length_24568_cov_11.133847"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PLQ11_01725"
     CDS             <1..206
                     /locus_tag="PLQ11_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiB"
                     /protein_id="HRK23650.1"
                     /translation="HAARLPDTLETAGRVLARLETASQDGFRLSEASIEAIGRAEGRG
                     NRTGSLALWALVALAAYAVFFGG"
     gene            210..1427
                     /gene="coaBC"
                     /locus_tag="PLQ11_01730"
     CDS             210..1427
                     /gene="coaBC"
                     /locus_tag="PLQ11_01730"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017484238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HRK23651.1"
                     /translation="MATAGLGGKRILLIIGGGIAAYKTLDLIRRLKERGVGVRAILTK
                     AGGEFVTPLSVAALTGEKVQTDLFSLTDEIEMGHIELSRSADLVVVAPATADLMAKMA
                     QGLANDLASTALLATDKPVLLAPAMNVRMWQHPATQRNLAQLKADGIRLVGPNDGAMA
                     CGEFGPGRMAEPMEILAAIEQALAPAGKGPLAGRHVLVTSGPTHEPIDPVRYIANRSS
                     GKQGHAIAAAAAAAGARVTLVSGPVTVPDPAGVRVVRVESARDMLAAVEAALPADIAI
                     FAAAVADWRVARSAEEKIKKGAGGPPSLALVENPDILATIARSGKRRPPLVVGFAAET
                     EAVIEHARAKLARKGCDLIVANDVSPETGIMGGDRNTVHLVASDAVETWPDLPKTEVA
                     RRLVAALAKRMMP"
     gene            1424..1909
                     /gene="dut"
                     /locus_tag="PLQ11_01735"
     CDS             1424..1909
                     /gene="dut"
                     /locus_tag="PLQ11_01735"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HRK23652.1"
                     /translation="MTLRVAFRQLAHGAGLPLPAYATKGAAGLDLAAAVEVPLVLAPG
                     ARALVPTGLAMALPSGYEAQLRPRSGLALKHGVTVLNAPGTIDCDYRGELKALLINHG
                     EEPFTIERGMRIAQLVVAPVTTIRVDLVADLDETERGDGGYGSTGGIRAFSSEVDSGS
                     H"
     gene            2110..3072
                     /gene="cysK"
                     /locus_tag="PLQ11_01740"
     CDS             2110..3072
                     /gene="cysK"
                     /locus_tag="PLQ11_01740"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003527809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HRK23653.1"
                     /translation="MSQVSKPGRGRIFSSITETIGDTPLVRLDRLAAEAGLKARLIAK
                     LEFFNPIASVKDRIGVAMIEAAEKAGTIAPGRTTLVEPTSGNTGIALAFVAAARGYRL
                     ILTMPETMSVERRKMLAYLGAELVLTEGAKGMKGAIAKAEELIATIPGSVMPQQFQNP
                     ANPAIHRATTAEEIWNDTGGQIDVFLAGVGTGGTITGVGQVLKSRRPELKVIAVEPEE
                     SPVLSGGAPGPHKIQGIGAGFVPDILDTRVIDEVIKVSSATAIETARKLARLEGIPGG
                     ISSGAATAAALQVASRPEMAGKTIVYIVPSFAERYISSLLFEGL"
     gene            3183..3259
                     /locus_tag="PLQ11_01745"
     tRNA            3183..3259
                     /locus_tag="PLQ11_01745"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3217..3219,aa:Arg,seq:ccg)
     gene            complement(3463..4467)
                     /locus_tag="PLQ11_01750"
     CDS             complement(3463..4467)
                     /locus_tag="PLQ11_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014762816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HRK23654.1"
                     /translation="MDFAVFSMLWLGKPLWMWLLFLGIVIALLVFDLGILHGTEREIG
                     VRESLLLSTMYISLGLAFGGWVWWQLGAESGINYLTGFVVEKTLALDNVFVIALIFSF
                     FAVPRQYQHRVLFWGILGVIVLRAIMIGVGAVLVEQFSWLLYVFALFLIITGIKMLYL
                     GDHPPEVGNNVVIRFMRNRFNVTDRLHGKAFLVRQPHPVTGKPALFITPLFMALVMVE
                     IVDVVFAVDSVPAIFAITTDPFIVYTSNIFAILGLRALYFALAAMIHRFRYLKPALAI
                     VLIFIGSKIFIADLLALQKFPAALSLAITFAIIAAGVIWSLVKTRDEAEKANKVPGLQ
                     "
     gene            complement(4572..4781)
                     /locus_tag="PLQ11_01755"
     CDS             complement(4572..4781)
                     /locus_tag="PLQ11_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HRK23655.1"
                     /translation="MDSYLTALRCKRDKIQADIDAEYARPCPDGLILTELKKAKLKLR
                     ERIEQAERSRLGPAFAALPAQTQPA"
     gene            4880..5800
                     /locus_tag="PLQ11_01760"
     CDS             4880..5800
                     /locus_tag="PLQ11_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-inducible genes activator"
                     /protein_id="HRK23656.1"
                     /translation="MPYRPTFRQLEYVAALDDLRHFGQAAARCHVSQPTLSVQIAQLE
                     RHLATPLFDRASFGVTPTPVGAEIARRARLLLAQLDDIVALSEADRLRLGGLIRLGTA
                     PSFGPYFLPQLVRQVHGKYPALKLYIREDRPLDIERAVAEGQIDCAVGPEPHEAGLVF
                     RAVGAERIWVGVPTEDPLAARPVLSLADLAGARFLSLGAGHRLAEILRELADAAGAHI
                     IDDYEGTSLDAIRQMVSIGMGLSIFPELYARSEFRGDDGVSLRPIAGWAGRRAVGLFW
                     REGAGRAAHYLALASEADGIAERLGLTGTG"
     gene            6126..7655
                     /gene="rpoN"
                     /locus_tag="PLQ11_01765"
     CDS             6126..7655
                     /gene="rpoN"
                     /locus_tag="PLQ11_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HRK23657.1"
                     /translation="MAIAPKLVMRQSQSLVMTPQLLQAIKLLQLSTSELSAFVEAELE
                     RNPMLERADSEEEAPFQTPSESVAETREEGDWALDRMPADRQTLERDLGTELENAFPD
                     DAPPTPAASVSDAPADSPFWDVRGGGGGIALDGDDPNLEAYLAASTSLAEHLHHQLAE
                     TLAEGTERLIGAVIIEAIDPTGYLAEPLSDIARRLGVSIEEVEAVLGIIQGFEPSGIG
                     ARSLAECLAIQLRERDRFDPAMQVLVGHLDLLAKRDFAGLRRLCQVDEEDLADMVREI
                     RALDPKPGLAFSAPPAEPVVPDVIVRSAPDGSWRVDLNSEALPRVIANRSYYARISRD
                     KEMAVRSFADEAWSNATWIVRSLEQRARTVLKVATEIVRQQDGFFAYGVAHLRPLNLK
                     TIADAVQMHESTISRVTANKFMATPRGLFEMKYFFTTAIASADGGEAYSAEAVRHRIR
                     QMIEAEAPDRILSDDAIVDALRSIGIDIARRTVAKYREAMHIPSSSVRRREKSVARAS
                     L"
     gene            7748..8302
                     /gene="raiA"
                     /locus_tag="PLQ11_01770"
     CDS             7748..8302
                     /gene="raiA"
                     /locus_tag="PLQ11_01770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HRK23658.1"
                     /translation="MSLRVTGKNLDIGDVLRNQAEEKINEVIAKYADGGYSGHVTVVK
                     DGQGFRTDCTLHLGNGQDLHVAGEAADAYASLSVAVERIAKQLRKIKRRRSDHAGHGA
                     GARPPEPALHDDDDVDVVETTSAGAAIVAEPVANARTLTVLGAVAAFEGNQAPVLVFR
                     NAGTGRINVLYRRADGHYGWVDIG"
     gene            8428..8892
                     /gene="ptsN"
                     /locus_tag="PLQ11_01775"
     CDS             8428..8892
                     /gene="ptsN"
                     /locus_tag="PLQ11_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HRK23659.1"
                     /translation="MKLGSILRPDMIAGGLKANSKKQALQELAELAARDTGLSERDVF
                     DALLQRERLGSTGIGSGIAIPHSRMPQAKRLFGAFARLDKPIDFESVDGIPVDLVFVL
                     LAPEDAGADHLQGLARVARLLRTPNVVQALRATRDPAALYSILTVGDTSRAA"
     gene            complement(9090..10340)
                     /locus_tag="PLQ11_01780"
     CDS             complement(9090..10340)
                     /locus_tag="PLQ11_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23660.1"
                     /translation="MDTRENRAHESPAADAAGLLLRIGFGLLVLAAPVSAIYSRRAFV
                     VLVPIGALLIVGAGLVRDSADLLRHARAAVLSPIGGLLLLFSLWLLLSLIWTPFPGPA
                     GERALRTVGNLLMAVMVAHALPERMRASNLHLMTIGVAAATLALSLSALVGPYAFRAL
                     HNPETPTLGRAAVAASVMVWPAVAWTHLRNRVWHGLALVVVCALAVAASGSPDALVTL
                     VVALAVYLAAQRWPMASGRGLAFLAAGIVLFAPLLALAARALVTTTILADRSLLAQAA
                     SWAPDLLQEPLRLLTGHGFDTSHRASKAGLVGQQAQDGLLQMLWFDLGLPGALLCAMV
                     LFFTFRTLAERPRPLAPAAMTVLATSFVVAMIDPSATQAWWLSVCVVTAVMLSAVARG
                     QYRTARPAANVVAPALRPAEPAAG"
     gene            10484..11050
                     /locus_tag="PLQ11_01785"
     CDS             10484..11050
                     /locus_tag="PLQ11_01785"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HRK23661.1"
                     /translation="MTIAYDALIQRRSASAKHLVAPGPETSVLHRILFAASRAPDHGR
                     LHPFRFVIIRPESRVRFADVLAEAARQDRPDLPETEIGRAREKAHQGPCLIGLIARID
                     TAHPKIPASDQWLTVGCALENLLLATAAEGFAAAVRSGRSLEAPAIREAFALGPNEHF
                     TCFIALGTPSEWPPAKPKPDVAELTSQW"
     gene            complement(11110..12984)
                     /gene="phnD"
                     /locus_tag="PLQ11_01790"
     CDS             complement(11110..12984)
                     /gene="phnD"
                     /locus_tag="PLQ11_01790"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_function: GO:0043225 - ATPase-coupled inorganic anion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HRK23662.1"
                     /translation="MALRRVVVGITGVLLAGTAVVAQQVIVVPDNRAVPAAQVQRVTQ
                     GAIDTRAPAPGTFVIPENKVVIPPAPGLVPYVPADGRIAGAPQAFYPQDFRTQRTEVP
                     IVAGEARTGWSEHRGQGDPNIRARPQDSRPMVAATSSGPVDYRAAAQRVSTQPPPAYV
                     PAEAPAVPAFTPPAIPPGMVIERTAAQPGYLRQPSADPRVAEPVRPAPPRLAPGVVVP
                     AATVQEPQLPVYEAKPGVIDPRVVIPREEKYIDYKPVEGRPYDFRPLDFKPVDTRAME
                     YRSLENKIQDQKVLDPRLKDKGPEIQARLECKAILYRAVECNFLDYKEVDPRLQELFA
                     KYPELTIGVVPKEYSKETPDRWAGLMTHLSREIGLKVSLKIANDYQALIESQRAGLIH
                     IAVYSPLAYARARNTGSKVEAFAVETNGDGTRGSHAVIYALNRGAAAKVEDYRGKSIG
                     LVDPNSISGYSVPRFAVMGQKLDPDSVFSRQVFAGSHENALVALSQGLVDYAVGQWTS
                     DEDSTLAKLLARGALRNVDGTPMRRDDFRVVLKSELIINSPIAYLSEMPEDIKATIRK
                     AFLEAPMRDRSAFEKIYESKGRSWETIEASAYDNTIDLVRFIDDAKRLQATANKQAAR
                     "
     gene            complement(13163..14041)
                     /gene="mmsB"
                     /locus_tag="PLQ11_01795"
     CDS             complement(13163..14041)
                     /gene="mmsB"
                     /locus_tag="PLQ11_01795"
                     /EC_number="1.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008442 - 3-hydroxyisobutyrate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyisobutyrate dehydrogenase"
                     /protein_id="HRK23663.1"
                     /translation="MTTIAFIGLGNMGGPMAANLIKADHSVTGFDLSDASKAAAQEAG
                     VAIAADARAAVKGASVVVTMLPAGKHVRAVWADLVAAAGKGTLFIDSSTIDVESARAV
                     HKLAAEAGMASIDAPVSGGTGGAKGATLTFMCGGEASAFDRAKPILEKMGKRIVHCGG
                     AGAGQAAKICNNMILGISMIAVSEGFSLAEKLGLSHQALFDVASTSSGQCWSLTTYCP
                     VPGPVPTSPANNGYKPGFAAELMLKDMKLAIEAASASGASVPLGAAAAQLYQLFNASG
                     HAGDDFSGIINMIRGA"
     gene            complement(14043..14546)
                     /locus_tag="PLQ11_01800"
     CDS             complement(14043..14546)
                     /locus_tag="PLQ11_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012651423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23664.1"
                     /translation="MPEAKRPNLVGAAGEHYVMCQLLRRGMIAALAPQGVPNADIVVT
                     DEIGDRLCAIQVKTRRDIGSDRGWHMKAKHEDIVGDRLFYCFIDFGKSLITPPKCWVV
                     PSAIVASILKSSHGNWLEQPGKKGQPHKDGEMRRFRPDYSELGLGSTHGEGWLEKYYE
                     AWNLLED"
     gene            complement(14554..15690)
                     /locus_tag="PLQ11_01805"
     CDS             complement(14554..15690)
                     /locus_tag="PLQ11_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRK23665.1"
                     /translation="MSFALNDDQIAIRDMARSFADEKIAPFALKWDEEKHFPIDVIKQ
                     AAGLGMAGIYVREDVGGTGLGRLEAALIMEQLATGCPAVSAYISIHNMVSWMIDKYGS
                     ETLRQTWLPRLTTMDLLSCYCLTEPGSGSDAAALRTTARREGDHYVLNGTKQFISGAG
                     AGDLYLIMVRTGAEGPKGISAVLVEKGTPGLSFGPNEKKMGWHAQPTRQVIMEECRIP
                     VANLIGEEGQGFKIAMAGLDGGRLNIGACSLGGAQSAIEKAVAYAAQRKAFGKAINEF
                     QSLQFRLADMATKLEAARVMLYSAASAYDRQDPETTVRCAMAKRFASDTGSEVVDDAL
                     QIHGGYGYLHEYGVEKLARDLRVHRILEGTNEIMRLIVARKMIG"
     gene            complement(15766..16083)
                     /locus_tag="PLQ11_01810"
     CDS             complement(15766..16083)
                     /locus_tag="PLQ11_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23666.1"
                     /translation="MSALFLTPLVIANRLPQLWFEALHPNPLVSDESRLAVTEKVEAF
                     TESFVGAHLATLTAPFTVAVDLLNGRSMMGAALGVQKKIAHATVQPIEKRLRHNLRRL
                     RRR"
     gene            complement(16166..17662)
                     /locus_tag="PLQ11_01815"
     CDS             complement(16166..17662)
                     /locus_tag="PLQ11_01815"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002964909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102662 - malonate-semialdehyde
                     dehydrogenase (acetylating, NAD+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HRK23667.1"
                     /translation="MITYGHFIGGKHVPGTSGRFSEVFQPLDGSVRGKVALASKADLD
                     AAVADAAAAQPAWAATNPQRRVRVLMKFLELIARDNDKLADCLAREHGKTIPDAKGDI
                     QRGVEVVEVCLGAPHFLKGEFTDGAGPGIDIYSMRQPLGVVCGITPFNFPAMIPLWKL
                     APAIACGNAFILKPSERDPGVPMMLAELAIEAGLPQGILNVVNGDKEAVDAILDHPDI
                     KAIGFVGSTPIAEYIYSRGCANGKRVQCFGGAKNHMIIMPDADMDQAVDALVGAGYGA
                     AGERCMAISVAVPVGEKTANELVRRLIPRVESLKVGPSTDGSADFGPLVTAAHRDKVK
                     GYVDLGVKEGAKLVVDGRGFKMQGYEKGFYLGGCLFDHVTTDMRIYKEEIFGPVLSVV
                     RARDYAGAVKMINDHEYGNGTAIFTRDGDAARDFAARINVGMVGINVPIPVPLAYYTF
                     GGWKRSGFGDLNQHGPDAFRFYTKTKTVTARWPSGIKDGADFVIPTMQ"
     gene            17758..18645
                     /locus_tag="PLQ11_01820"
     CDS             17758..18645
                     /locus_tag="PLQ11_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019995048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRK23668.1"
                     /translation="MDWDHARVFLAVARAGQFLAAARHLRLDHATVGRRIGALEDGIG
                     ARLFERRTNGCVLTPAGEKFLIAAERVEAEMLKAQADLSEADVAVAGTVRIGAPDGFG
                     TLFLSGRLGRLMVRHPRLTIQLVPLPRTFSLSKREADIAIVIDRPEEGRLHVRKLTDY
                     TLGFYAAATYLCEHEEPRSIEALTGHRLVTYVQDLQFAGALNYFPQGFEPRFQRFECA
                     GVLGQFEAVRSGAGIGILHDYHARSDPSLRPVLPDHRIQRTYWLVTHDDTRSLARVRA
                     VAEFIGDEVAGAARAFLPQ"
     gene            18759..19745
                     /locus_tag="PLQ11_01825"
     CDS             18759..19745
                     /locus_tag="PLQ11_01825"
                     /inference="COORDINATES: protein motif:HMM:NF017773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRK23669.1"
                     /translation="MTLMHSRRAALSSLVALPLAGCWAGPYSSHEGAALAARTNDAPV
                     LHVITSRRLAGSGAQPPYFDWRRAAELTYAEARYARPDRSVLGRIGSVVSGDFGMSEV
                     SPFAGEALDRLAEVLSSRDSLLFVHGYNQTFEAAARDAARLGDDIGFDGRTILFTWPS
                     KGGLFDYGYDRESAMLARDHLADTLHALLGEGRSGRVHLVAHSMGTLATLEALRGYRD
                     RHGEAGLERLGTIVLAAPDIDFDVFKAGLSRLGPLRQKLTVITATNDRALDLSRRLAG
                     GDRVGALSGEQLVGLGVRIVDATDFASGLTRHDAFISNDDVRSVIRRAIERS"
     gene            19845..21116
                     /locus_tag="PLQ11_01830"
     CDS             19845..21116
                     /locus_tag="PLQ11_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23670.1"
                     /translation="MRAKSYLAGILSGLGFVCSAYASDAPSPAPLTPDIGLRSYIDIA
                     PDYSDRILDWQWDVNDKAVQPLEAIKNGQLAPGKLYVGGLVRWSYSNEHSNTLGKFPF
                     LGQFPDQHKKQFRIGETMFNNTHLGLTLVPAHWIAGSAEVIYTDKTYPGQDNVQIRKA
                     FISVGDLKTFPIYASFGKNSVDFGDMRSFNPFVHSMTNHYFNVHSEDAVGMVGYFRDG
                     WHVTASAIGGGRQLRVADSPSKRGSIGNFAINGSKTFEFADGFRVKLGGGYIDNTIYN
                     TPTPHHTSRQVAASGARVHNGAWDVNGMVSYKGFDVQTEYTSTVGRWPATDHPVSTLT
                     VQGRYTTDLWGWQTIISAGFSRGEQGADNTRWQQMRQIVAGLEIKPHKQLSLLMEYAH
                     LSGFVPLVAIKSVADPGVRSDVWVFGARAHF"
     gene            21260..21970
                     /locus_tag="PLQ11_01835"
     CDS             21260..21970
                     /locus_tag="PLQ11_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRK23671.1"
                     /translation="MTSRPESPFKFEKADIKMSVDKGEFRLQLKGPVINLKLKKPDFK
                     LKKPDIKLDDDTRFVLSWLGSPLKTGAVSPSGKELARAMAEPIDPAIPGSIIELGPGT
                     GPVTEALLARGIAPERLILIEYNTDFCKLLRQRFPGVQVIQGDAYNLAATLAGQVENK
                     VAAVVSSLPLMTRPDPECARLAREAFAMMAPDAPFIQFTYGPTSPVPLKGQPFRGRAV
                     KRVWKNLPPARVWVYRAM"
     gene            22095..22667
                     /locus_tag="PLQ11_01840"
     CDS             22095..22667
                     /locus_tag="PLQ11_01840"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020180962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HRK23672.1"
                     /translation="MSVKILVFSGSSRAGSVNDRLAGIVARQLQGMGAEVTYINLGKF
                     DLPMVNADGYGSAAPKAAHDLRAIIDEHDGLFIVSPEYNAGYTPLLKNALDWASVAKP
                     GAPASGLSGKVVALGAVSPGPMGGYRSLTQLRTVLELGFGATLVPEMVAVGNAGSAFA
                     ESGGLADERTAGFLNATLSKLITMTKKLKA"
     gene            22664..23254
                     /locus_tag="PLQ11_01845"
     CDS             22664..23254
                     /locus_tag="PLQ11_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007633885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRK23673.1"
                     /translation="MIGTHHLYLTHPQVTIDPAVPVPLWGLSAVGRARMERLAGEGWI
                     RRFGRIVSADETKALEAAAILAAALGCQVEQRGGLHENDRSATGFLPPPEFEATADRF
                     FADPDRSIRGWERAVDAQQRIVTGVRAIIGEHPDLPTIFVGHGGVGTLLRCALRGIAI
                     SRKEDQGPGGGGCHFAFALEPVSVFYPWRPLEIPPA"
     gene            23266..23967
                     /gene="pyrF"
                     /locus_tag="PLQ11_01850"
     CDS             23266..23967
                     /gene="pyrF"
                     /locus_tag="PLQ11_01850"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HRK23674.1"
                     /translation="MPVPPRERLIVALDLPGVEPARAMVARLGDKVSFYKIGKELVYA
                     GGLDLARDLVAAGKKVFLDLKLHDIPATVERATAAVSDLGATYLTVHAYPQTLAAAVR
                     GKGDSNLRILGVSVLTSMNDDDLAKAGYGLGVSDLVRRRARQAYEAGAAGLICSPLDI
                     AIVRDSVGDGLEIITPGVRPAGSDHGDQKRVLTPGEAIRAGADRLVVGRPITGAADPA
                     AAAAAIVAEIAGAAA"
     gene            24145..24453
                     /locus_tag="PLQ11_01855"
     CDS             24145..24453
                     /locus_tag="PLQ11_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HRK23675.1"
                     /translation="MTKGYWIARVDVYDAEAYKNYVAHNSAAFKKYGARFLVRGGQFA
                     LKEGMARSRNVVIEFPSYQAALDCFHSPEYQHALSFRRGPVSAGDVVIIEGYDGPQPG
                     "
     gene            24460..>24568
                     /locus_tag="PLQ11_01860"
     CDS             24460..>24568
                     /locus_tag="PLQ11_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HRK23676.1"
                     /translation="MSDLKLAVVGAAGRMGRMLVRAAIEPDGVALAGALE"
BASE COUNT         4474 a         7691 c         7799 g         4604 t
ORIGIN      
        1 gccatgccgc ccggctgccc gacacgctgg agaccgccgg gcgggttctt gcccggctcg
       61 agaccgcctc gcaggatggg ttccggctgt cggaggccag catcgaggcc atcggccggg
      121 ccgaagggcg cggcaaccgc accggttcgc tggcactgtg ggcgctggtg gcgctggcgg
      181 cctatgcggt gtttttcggg gggtgaggca tggcgacagc ggggcttggc ggcaaacgca
      241 tccttctgat catcggcgga ggtatcgccg cctacaagac gcttgatctc atccgccgcc
      301 tgaaggagcg cggcgtcggt gtgcgcgcca ttctgaccaa ggccggcggt gaattcgtca
      361 cgccgctatc ggtggccgcg ttgaccggcg aaaaggtgca gaccgatctg ttctcgctca
      421 ccgacgagat cgagatgggg catatcgaat tgtcgcgctc ggccgatctt gtcgtggtgg
      481 ctccggcgac cgccgacctg atggcgaaga tggcgcaggg ccttgccaat gatctggcct
      541 cgaccgcgct gctcgccacc gacaagcccg tcctgctggc cccggcgatg aacgtgcgga
      601 tgtggcagca tccggcgacg cagcgcaacc ttgcgcagct caaggctgat ggtatccggc
      661 ttgtcgggcc caatgacggg gccatggcct gcggcgagtt cggccccggc cgcatggccg
      721 agccgatgga gatcctcgct gccatcgaac aggcgctggc tcctgccgga aaagggccgc
      781 tcgccggccg ccatgtgctg gtgacctccg ggccgaccca tgaaccgatt gacccggtgc
      841 gctacatcgc caaccgttct tccggcaagc agggccatgc catcgccgcc gccgccgcgg
      901 cggcgggtgc gcgggtgacc ctggtctccg gtcccgtcac tgttcccgat ccggcaggcg
      961 tgcgggtcgt gcgtgtggaa agcgcgcgcg acatgctggc agcggttgag gccgccctgc
     1021 cagccgacat tgccattttc gccgccgccg tcgccgactg gcgcgttgca cgctcagccg
     1081 aggagaagat caagaagggc gcgggtggcc cgccctccct ggcgctggtt gaaaaccccg
     1141 acattctcgc caccatcgcc aggagcggca agcgtcgccc gccgctggtt gtcggctttg
     1201 ccgcagagac cgaggcggtg atcgagcacg cccgggccaa gcttgcgcgc aagggctgcg
     1261 acctgattgt tgccaacgat gtcagcccgg aaaccggcat catgggcgga gaccgcaaca
     1321 ccgtgcatct ggtcgccagc gacgcggtcg aaacctggcc tgacctgccg aaaacagagg
     1381 tggcccgccg ccttgttgcc gcgcttgcga aaaggatgat gccatgaccc tgcgcgtggc
     1441 gttcaggcag cttgcgcatg gcgccggtct gcccctgccg gcctatgcca caaagggcgc
     1501 agccggcctc gatctcgccg ctgcggtcga agtgccgctg gtgctggcgc ccggcgcgcg
     1561 ggcgctggtg ccgacaggct tggcgatggc gctgccaagc gggtatgagg cccagcttcg
     1621 gccccgttcg gggctggcgc tcaaacatgg cgtgacggtg ctcaatgctc ccggcaccat
     1681 cgattgcgat tatcgaggcg agttgaaagc cctgctgatc aatcatggcg aggagccgtt
     1741 caccatcgag cgcggtatgc ggatcgccca gctcgtggtc gcgccggtga ccacgattcg
     1801 ggtggacctg gtggccgatc tcgacgagac ggaacgcggc gatggcggct acggctcgac
     1861 cggagggatt agagcatttt caagcgaagt ggattccggt tcgcattaag aaaatgcgat
     1921 aaaacagaaa gatagagcac tttatgtgaa ttggagttcg cccaccatgc tccagcagaa
     1981 aatagttctt tcttggggca attctgtagt ccgtattgct gttcgaggcc ggaaaataac
     2041 aagtcgtcga acattgtttc gcagcacggg ctggcatagg ctgcggctcc ccctttgccg
     2101 gagagcatca tgtcacaggt aagcaagccg ggtcgcggcc gcatcttttc ctcgatcacc
     2161 gagaccatcg gcgatacgcc gctggtgcgg ctcgaccggc tggcggcaga ggcggggttg
     2221 aaggcaaggc tgattgccaa gcttgaattc ttcaacccta ttgcctcggt gaaggatcgc
     2281 atcggcgttg ccatgatcga ggcggcggag aaggccggga cgattgctcc tggccgaacc
     2341 acgctggttg agccgacttc cggcaacacc ggcattgcgc tggccttcgt tgcggctgcg
     2401 cgtggctacc ggctgattct gaccatgccg gaaaccatgt cggtggagcg gcgtaagatg
     2461 ctcgcctatc tcggcgccga actggtgctg accgaaggcg caaagggcat gaagggcgcc
     2521 atcgccaagg ccgaagaact gattgcgacg attcccgggt cggtcatgcc acagcagttc
     2581 cagaacccgg ccaatccggc cattcaccgc gccaccaccg ccgaggagat ctggaacgac
     2641 accggtggcc agatcgacgt gtttctggcc ggcgtcggca ccggcggcac catcaccggc
     2701 gtcggccagg tgctgaaatc ccgcaggccg gagctgaaag tcatcgcggt cgagccggag
     2761 gaaagtccgg tcctgtccgg tggcgcgccg ggtccgcaca aaattcaggg gatcggcgcc
     2821 gggttcgtgc cggatattct cgataccagg gtgatcgacg aagtgatcaa ggtgtcgagc
     2881 gccaccgcca tcgagaccgc gcgcaagctc gcgcggcttg aaggcattcc cggcggcatt
     2941 tcctcggggg cagcgaccgc agcggcgctg caggtggcca gccgccccga gatggccggc
     3001 aagacaatcg tctacatcgt gccctcgttc gcagagcggt atatctcaag cctgctgttc
     3061 gagggattgt gagggcgtgc cctgccggat gcggcactgg cggctcgcgg tccctgcaag
     3121 aacgacgggc gagacggttg ctccggcagc cggactcgtc tattgcgtga gcagtcacga
     3181 ctgcacccgt agctcagctg gatagagcgt tgccctccga aggcaaaggt cacacgttcg
     3241 aatcgtgtcg ggtgcgccaa tgatttctac aaggtcacaa ggtcacaagg tcacaagatt
     3301 gcggcggatc gctaccaccg cccgtcatga ccttaagcca ttcccggtcc ggtgaccggc
     3361 aagacggcgt gaccccagga tcgcgcagtg ttcgggctcc ctgtcgcagg actgaaacca
     3421 cctgtccgcc gacggcgggc agcgagattg atacgtcttt catcattgaa gacccgggac
     3481 cttgttcgcc ttctcggcct cgtcgcgcgt cttgacgagg ctccagatga cgcccgctgc
     3541 gatgatggcg aaggtgatcg ccagcgaaag cgcggcaggg aatttctgca aggcgagcag
     3601 atcggcgatg aatatcttcg agccgatgaa gatcagcaca atggccagcg ccggcttgag
     3661 atagcggaaa cgatggatca tcgcggcaag ggcgaaatac agggcgcgaa ggccaaggat
     3721 ggcgaagata ttggaggtgt agacgatgaa cgggtcggtg gtgatggcaa agatcgccgg
     3781 caccgaatcg accgcaaaaa ccacgtcgac gatctccacc atcaccagcg ccatgaacag
     3841 cggtgtgatg aacagggcgg gcttgccggt caccggatgc ggctggcgca ccaggaaagc
     3901 cttcccgtgc agccgatcgg tgacgttgaa ccggttgcgc atgaagcgaa tgacgacatt
     3961 gttgccgact tcgggcggat gatcgcccag atacagcatc ttgatgccgg tgatgataag
     4021 gaacagggca aaaacataga ggagccacga gaattgctcc accagcacgg cgccgacacc
     4081 gatcatgatc gcacgcagca cgatgacgcc cagaatgccc cagaacagta cgcgatgctg
     4141 gtattgccgc ggcactgcga agaacgagaa gatgagcgcg atgacaaaga cattgtcgag
     4201 cgccagggtc ttttcgacga caaaacccgt cagatagttg atcccggatt cggcgccgag
     4261 ttgccaccat acccagccgc cgaaggcaag gccgaggctg atatacatcg tcgacagcag
     4321 cagcgattcg cgcacaccga tttcgcgctc ggttccgtgc agaatgccga ggtcaaagac
     4381 aagaagagcg atgacgatac ctaggaacag cagccacatc cacagcggct tgcccagcca
     4441 gagcatcgag aagacagcaa aatccatggg gtcgatatcc aggtaaaacg gccacatcgc
     4501 gggccaaaat gcacctaata cccggcctca gcagggtact gagtgcggac tgctgggctt
     4561 ttggggtgag attacgctgg ctgcgtctga gcgggcaatg ctgcaaaggc ggggccgagc
     4621 cgagagcgct cggcctgttc gatacgttcg cgcaacttga gctttgcctt cttgagttca
     4681 gtcaggatga gcccgtcggg gcagggccgg gcatattccg cgtcgatgtc ggcctggatt
     4741 ttgtcgcgct tgcatcgaag cgccgtcagg tagctatcca ttgcggtctc ctccttgagc
     4801 catgaagagg acaatgggcg atgcctttcg attgagcaaa ttgattgtaa taatacattt
     4861 gatagtttag aactatcgaa tgccctatcg cccgacattt cgtcagctcg aatatgtggc
     4921 cgcactggat gatctgcgcc atttcggcca ggctgcggct cgctgtcatg tctcacagcc
     4981 gacgctgtcg gtgcagatcg cccagcttga gcgccacctt gccaccccgc tttttgaccg
     5041 ggccagtttt ggtgtgacgc ccacaccggt cggggcggag attgcccggc gcgcacggct
     5101 tctgctggcg cagctcgacg atatcgtggc cctgtcggaa gctgaccgcc tgcgtctcgg
     5161 cggtctgatc cgcctcggca cggcgcccag cttcgggccg tatttcctgc cgcagctcgt
     5221 gcgtcaggtg catggaaaat acccggcgct gaaactctat atccgcgagg atcgcccact
     5281 ggacatcgag cgggccgttg cggagggcca gatcgattgc gcggtcggtc cggaaccgca
     5341 tgaagccggg ctggttttcc gcgcggtcgg tgcggaaagg atatgggttg gcgtgccgac
     5401 ggaagatccg ctggcggcca ggccggtgct tagccttgcc gatctcgccg gcgcacgctt
     5461 tctgagtctg ggcgccggcc atcggctggc cgagatcttg cgcgaactcg ccgatgcggc
     5521 aggggcgcat atcatcgacg actacgaagg caccagcctt gatgccattc gccagatggt
     5581 gtcgatcggc atggggctgt ccatcttccc ggagctttat gcacggtcgg aattcagggg
     5641 tgacgacggg gtctcgttgc gtccgatagc cggatgggcc ggtcgtcgcg ctgtcgggct
     5701 gttctggcgc gagggggcag gacgggccgc ccattacttg gcgctggcca gcgaagcgga
     5761 cgggatcgcc gagcgcctgg ggctgacagg gaccggttga aggatccgac ctgatgccat
     5821 caggtcgcca gccattcccg gccatggtgg gccaacctcg gccaaacggc ctgtgacgct
     5881 gggtcggcca ctttcatttt tggtcaagca agtttcatgc tagtttgcgg acggcaaggg
     5941 tccggcaacc ttcagggcgc tattgctgta gtcctatgat cacgctctcg ttttgataag
     6001 gcaatcgaaa cgagggataa cctgatcgat ctcaacaggt tagagcatgc ttgttgcgat
     6061 tggccgggct ccacctaatc gcagcatgct ctagcagcgt gaacctcggg tctcgacagg
     6121 tcagtatggc gattgccccc aaactcgtga tgcgtcagag ccagtcgctg gtgatgacgc
     6181 cgcaattgct gcaggcgatc aagctgctgc agttgtcgac gagcgagctg tcggcctttg
     6241 tcgaagccga gcttgagcgc aatcccatgc tcgagcgggc agactcggag gaggaagcgc
     6301 cctttcagac gccatcagag agcgtggcgg aaacccggga agagggtgac tgggcactcg
     6361 accggatgcc ggccgatcgc cagacgctgg aacgcgacct tggcaccgaa cttgaaaatg
     6421 cctttcctga cgatgcgccg ccaaccccgg ccgccagtgt cagcgacgcg ccggccgaca
     6481 gcccgttctg ggatgtgcgg gggggcggag gcgggattgc gctggatggt gatgacccca
     6541 atctggaagc ttatctcgcc gccagcacct cgcttgccga acacctgcat caccagttgg
     6601 cggagacgct ggccgaggga accgagcgcc tgatcggcgc tgtgatcatc gaggcgattg
     6661 acccgaccgg ctaccttgcc gagccgttgt ccgacatcgc ccgccggctg ggggtctcga
     6721 tcgaggaggt cgaggcggtg ctcggcatca tccaggggtt tgagccaagc ggcattggcg
     6781 cccgctcgct ggccgagtgt ctggcgatcc agctcaggga gcgcgaccgt ttcgatccgg
     6841 ccatgcaggt gctggtcgga catctcgacc tgcttgccaa acgcgatttt gccgggctcc
     6901 ggcggctctg ccaggtcgat gaggaggatc tggctgatat ggtgcgggaa atccgcgcgc
     6961 ttgatcccaa gcccggcctt gccttcagcg cgccgccggc agagcctgtc gtgccggatg
     7021 tgatcgtccg ctccgccccg gatggcagct ggcgggtcga tctcaacagc gaggccctgc
     7081 cgcgcgtcat cgccaaccgc agctattatg cccgcatatc gcgcgacaag gaaatggcag
     7141 tgcgttcgtt tgccgatgaa gcatggagca acgccacgtg gattgtccgt tctctcgagc
     7201 agcgggcgcg caccgttctg aaagtcgcca ccgagatcgt ccggcagcag gatggctttt
     7261 ttgcctacgg cgtcgcccat ctcaggccgc tcaacctcaa gaccattgcc gacgccgtgc
     7321 agatgcatga atcgaccatc tcccgggtca ctgccaacaa gttcatggcg acgccgcgcg
     7381 gcctgttcga gatgaagtat tttttcacca ccgcgattgc ctcggccgat ggtggcgaag
     7441 cctattctgc cgaggccgtg cgccatcgca tccgccagat gatcgaggcc gaggcgccgg
     7501 atcgcatcct gtcggatgac gccatcgttg atgcgctccg cagcattggc atcgacatcg
     7561 cgcggcgcac ggtcgccaaa tatcgtgagg cgatgcacat cccctcttcc tcggtgcgcc
     7621 ggcgcgaaaa gtcggtggcg cgcgccagcc tgtgaagccc aggatgcctt ttattcccga
     7681 ttgcctgtga ctagactctc accatgcggc ggcagattcg ccgcccatct caaccaggga
     7741 acccttcatg tctctcagag tgaccggaaa gaacctcgac atcggtgatg tgctgcgcaa
     7801 ccaggcagaa gagaagatca acgaggtgat cgccaaatat gctgatggcg gctattccgg
     7861 ccatgtcacg gtggtcaagg atggccaggg ctttcgtacc gattgcacgc tccatcttgg
     7921 caacggtcag gacctgcatg tcgccggtga ggcggccgat gcctacgctt cgctgtcagt
     7981 tgcggtcgaa cggattgcca agcagcttcg caagatcaag cgccgccgat ccgaccatgc
     8041 aggccacgga gccggggcgc ggccgccgga accggcgctg cacgacgacg acgatgtcga
     8101 tgtggtcgag accacatcgg cgggagcggc tattgtggcc gaaccggttg ccaatgcccg
     8161 gacgttgacc gttcttggtg cggtggcagc ctttgagggc aaccaggcgc cggttctggt
     8221 tttccgcaac gccggcaccg gccgtatcaa tgtgctttac cgtcgtgccg atgggcatta
     8281 tggctgggtt gatatcggct gatcgtaact gtctggtggg ccccggcgat cggcctgggc
     8341 ccgcagctgt ggaagcagac ccatgatccc tgtgcaatcg ccggtccggc actggatcgt
     8401 gcacgccatt tcgactaagt agagctcatg aagcttggta gcatcctgcg ccccgatatg
     8461 atcgccgggg ggttaaaggc caacagcaag aaacaggcac tgcaggaact cgcagagctt
     8521 gcagcgcgcg atactggcct cagcgagcgc gacgtgttcg atgccctgtt gcagcgcgag
     8581 cggcttggtt cgaccggaat tggcagcgga attgccattc cgcacagccg tatgccacag
     8641 gccaagcgcc tgttcggtgc ctttgcccgg ctcgacaagc cgatcgattt tgaatcggtc
     8701 gacgggatcc cggttgatct tgtcttcgtg ctgctcgcgc ccgaagatgc aggcgccgac
     8761 catcttcagg gattggcccg cgttgcgcga ttgctgcgca ctcccaatgt ggtgcaagcc
     8821 ttgcgcgcca cccgcgatcc tgctgcgctc tattcgatcc tgacggtagg cgatacctcc
     8881 agagcggcct aataccaagt tgcaaggcga ggaactcgcc ttgacttggc tcgcttcaaa
     8941 aatttctttt ttatcaacga gatgagaaat tttgagttcc accaaagagt cattttcaat
     9001 gacttttggt ataaagcatt ttcaagcgaa gtagggtccg gttcgcgtta agaaaaggcg
     9061 ctttccggtg cgatcggctg tgacccgccc tatcccgctg ccggttcagc cggtcgcagg
     9121 gccggcgcca ccacattcgc cgccggcctt gcagtgcggt attgtcccct ggcgaccgcc
     9181 gacagcatca cggcggtcac cacgcacaca ctcagccacc atgcctgggt cgcgctcggg
     9241 tcaatcatcg ccacaacaaa agacgtcgcc agcaccgtca tcgccgctgg cgcgagcggg
     9301 cgtggccgtt ccgccagggt gcggaaggtg aagaacagca ccatggcgca aagcaaggcg
     9361 ccaggcaggc cgagatcaaa ccacagcatc tgcaacagcc cgtcctgcgc ctgttgaccg
     9421 accagcccgg ccttcgaggc cctgtggctt gtatcaaacc cgtgccctgt cagaagccgc
     9481 aggggttcct ggaggagatc cggcgcccag gatgcggcct gggccagcag cgaacggtcc
     9541 gccaagatgg tggttgtcac aagcgcccgc gccgccaatg ccagcagcgg agcgaagagc
     9601 acgatgccgg cagccagaaa ggccaggccg cgtccgcttg ccatcggcca gcgctgcgcc
     9661 gcaagataga ccgcaagggc gacaaccaga gtcaccagcg cgtccggcga gccgctggcg
     9721 gcgaccgcca aggcgcagac caccacaagc gccagaccat gccacactcg gtttcgaagg
     9781 tgtgtccacg caacggcggg ccaaaccatg acgctggccg cgacagcggc gcggccaagg
     9841 gtcggcgttt cagggttgtg caacgcgcga aaagcatagg ggccgaccag ggcggacagc
     9901 gacagcgcca gtgtcgccgc cgccacgccg atcgtcatca gatgcaggtt ggaggcgcgc
     9961 atgcgctcgg gcagcgcatg ggcgaccatc accgccatca gcaggttgcc gaccgtgcgc
    10021 agcgctcgtt cgccggccgg gccgggaaat ggcgtccaga tcaatgatag caacagccag
    10081 agcgagaaca gcagcaggag cccgccgatc ggcgacagaa cggccgctct ggcatggcgc
    10141 agaagatcgg cactgtcccg cacgaggcca gcgccgacaa tcagcagcgc gccgatcggc
    10201 accaggacca cgaaggcgcg acgcgaatag atggcggata ccggcgctgc cagcaccagc
    10261 aggccaaatc cgatgcgcag cagcaggccg gccgcatcag cagccggtga ttcatgagcc
    10321 cggttttcgc gcgtgtccat gtccgtcaac agcttccggt cagtctctgg tccttgccat
    10381 agcgatgctt atggcaaagt ctctggcgtt gctgtagggg gcttgccaca gtttcgccca
    10441 aaatggcacc cttacccgat ctgtcacgtc caaatggtgt tgcatgacca ttgcctatga
    10501 tgccctcatc caacgccgct cggccagcgc caagcacctc gtcgccccgg gtccggagac
    10561 ttcagtcctg catcgcatcc tgtttgccgc ctcgcgcgcc ccggatcatg gccggttgca
    10621 tccgttccgc ttcgtcatca tccggccgga aagccgcgtc cgcttcgccg atgtgctggc
    10681 agaggccgcc cggcaggaca ggccggatct gccggaaacg gaaatcgggc gcgcccgcga
    10741 aaaggcccat caggggccct gtctgatcgg gctgatcgcc cggatcgata cggctcatcc
    10801 caagatcccg gcgagtgacc agtggctgac cgtcggttgc gcgctggaga acctgttgct
    10861 ggcgaccgct gcggaaggat ttgccgccgc cgtccgctcc gggcgctcgc tggaagcgcc
    10921 ggcgatccgc gaggctttcg cccttggccc caacgagcat ttcacctgtt ttatcgcgct
    10981 gggcacgccg tccgaatggc cgccggccaa gcccaagccg gatgtggcag agctgacgtc
    11041 gcaatggtga aggggcggat tgctccgccc cctcgctgcc ggtcttgacg atcgacagaa
    11101 atcgtttcgt cagcgcgccg cctgcttgtt ggctgtcgcc tgcagccgct tggcatcgtc
    11161 gatgaagcgc acgagatcga tggtattgtc ataggcggag gcctcgatgg tttcccagct
    11221 gcggcccttg ctctcataga tcttctcgaa tgcggaccgg tcacgcatcg gcgcctcgag
    11281 gaatgccttg cggattgtcg ctttgatgtc ctcgggcatt tccgaaagat aggcgatggg
    11341 cgaattgatg atcagttcgg acttcaggac gacgcggaaa tcgtcgcgac gcatgggcgt
    11401 tccatcaaca ttgcgcaggg cgccccgagc cagaagcttg gccagggtcg aatcctcgtc
    11461 cgaggtccac tggccgacag cataatcgac cagaccctgc gagagcgcta ccagcgcatt
    11521 ttcatggctg ccggcaaaga cctgccggga gaagacgcta tcgggatcga gcttctggcc
    11581 catcaccgca aaccgcggca cggaatagcc ggatatcgaa ttgggatcga caagaccgat
    11641 gctcttgcca cgatagtctt cgaccttcgc cgcggcgcca cgattgagcg catagatcac
    11701 ggcgtggctg ccacgagtgc cgtcaccgtt ggtctcgacc gcgaaagcct caaccttgga
    11761 accggtgttg cgggccctcg cataggccag cggcgaatag acagcgatgt gaatgaggcc
    11821 agcccgctgg ctctcgatca gcgcctgata gtcattggcg atcttgaggc tgactttcag
    11881 gccgatctcg cgcgacagat gcgtcatcag ccccgcccag cgatccggcg tttccttgga
    11941 atattccttt ggaacaacgc cgatggtcag ctcgggatat ttggcgaaca gctcctgcag
    12001 gcgtggatcg acttccttgt aatcgaggaa attgcactcg accgcacgat aaaggatcgc
    12061 cttgcattcc agcctcgcct ggatttccgg ccccttgtcc ttgaggcgcg gatcaagaac
    12121 cttctgatcc tggatcttgt tctccagcga gcggtattcc attgcccgtg tgtcgaccgg
    12181 cttgaagtcg agcgggcgga aatcgtaagg acgcccctca acgggcttgt agtcgatata
    12241 cttctcctcg cgcgggatga cgacgcgcgg atcgatcacg ccgggtttgg cctcatagac
    12301 cggcagctgc ggttcctgaa ccgttgcggc aggcacgacc acgccgggcg ctaaacgcgg
    12361 gggtgccggc cgcaccggtt cggcaacgcg cggatcggcc gacggctgtc ggagatagcc
    12421 gggctgggca gcggtgcgct cgatcaccat gccaggcgga attgccggcg gtgtaaaggc
    12481 cggcaccgcc ggcgcctcgg ccggaacata ggccggtggc ggctgggtgc ttacgcgctg
    12541 ggcggccgcg cggtaatcaa ccgggccgga cgacgttgcg gcgaccatgg gccgcgaatc
    12601 ctgcgggcgc gcccggatgt tgggatcgcc ttgcccacgg tgctcgctcc agccggtgcg
    12661 ggcctcaccc gcaacaatcg gaacctcggt gcgctgggta cggaaatcct gcggatagaa
    12721 tgcctgcggc gcaccggcga tgcggccatc ggccgggaca tagggcacca gtcccggcgc
    12781 cggcggaatg accaccttgt tttcagggat gacgaatgtg ccgggcgcag gggcgcgtgt
    12841 atcgattgcg ccttgcgtga cccgctggac ctgggcggcc ggaaccgccc gattgtccgg
    12901 aactacgatc acctgctggg caacgactgc cgtgccggcc aacagcacac ccgtgatccc
    12961 caccacaacg cgacgcaacg ccatgacaca ctccgaaccc gtcgccggga aatgcgaccg
    13021 aacgcggcgc caccgtcaca cggcagacgc caagatcccc gcgtttaccc tgcgtcacga
    13081 atgctttgcg catggtaaaa acaggcttgc cggccccgtc tgccggagtg aacttcccgg
    13141 tagggttaac ggaacgtgtg gatcaggcgc cgcggatcat gttgatgatg ccggaaaaat
    13201 cgtcccccgc atggcccgac gcattgaaca gctgatagag ctgcgccgcc gccgcgccga
    13261 gcggcaccga ggctcccgag gcactcgccg cctcgatggc gagtttcatg tccttcagca
    13321 tcagttcggc ggcaaacccc ggcttgtagc cgttgttggc cggtgaggtc ggcaccggac
    13381 ccggcaccgg gcaataggtg gtgagactcc agcactggcc cgacgaagtg gaagcgacat
    13441 cgaacagcgc ctgatggctg aggccaagct tttcggccag cgaaaagcct tccgagaccg
    13501 cgatcatcga aatgccgagg atcatgttgt tgcagatctt ggccgcctgc ccggcgccgg
    13561 cgccgccgca atggacgatc ctcttgccca ttttttcaag gatcggcttt gcccggtcga
    13621 acgccgacgc ctcgcccccg cacatgaagg tgagcgtcgc gcccttggca ccgcccgtgc
    13681 cgcccgagac cggcgcatca atcgacgcca tgcccgcttc tgccgccagc ttgtggaccg
    13741 cgcgggcgct ctcgacatcg atggtcgagg aatcgatgaa cagcgtgccc ttgcccgccg
    13801 ccgcgaccag atcggcccag acggcgcgga catgcttgcc ggccggcagc atggtgacga
    13861 cgacagaagc gcccttgact gccgccctgg catccgccgc gatcgcaacg ccggcctcct
    13921 gcgccgccgc cttcgaggcg tcgctgagat caaacccggt taccgaatgg tcggccttga
    13981 tgagattggc cgccatcggg ccgcccatgt tgccgaggcc gatgaatgcg atggtggtca
    14041 ttttaatcct ccagaagatt ccaagcttcg tagtactttt ccagccaacc ctcgccgtga
    14101 gtgctgccta accccagttc cgaataatcc gggcggaaac gtcgcatttc accgtctttg
    14161 tgcggttggc ctttcttgcc aggctgctca agccaatttc catgacttga ctttaaaata
    14221 gaagccacga tggcgcttgg aacgacccag cactttggcg gcgtgatcaa tgactttcca
    14281 aagtcaatga aacaataaaa caggcgatct ccgactatgt cttcatgttt ggctttcata
    14341 tgccaacctc gatcagagcc aatatcccgc cgtgttttga cctgaattgc acacagtcgg
    14401 tctccgatct catcggtcac aacgatatcg gcattgggaa cgccctgagg cgccagagct
    14461 gcaatcatgc cgcgcctgag cagttgacac atcacataat gttcgccggc agcaccaacc
    14521 aaatttgggc gctttgcttc gggcatccta cgctcaccca atcatctttc ttgccacgat
    14581 caggcgcatg atctcgttgg tgccttcgag aatgcggtgc acgcgcaggt cgcgcgccag
    14641 tttctcgacg ccgtattcgt gcagatagcc gtagccaccg tgaatctgca gcgcgtcgtc
    14701 gaccacttcg gagccagtat cggaggcaaa gcgcttggcc atggcgcagc gcaccgttgt
    14761 ttccgggtcc tgccggtcat aggcggaggc ggcggaatag agcatcaccc gcgccgcttc
    14821 cagcttggtc gccatgtcgg cgagccggaa ctgcaatgac tggaattcgt tgatcgcctt
    14881 gccgaacgcc ttgcgctggg cggcataggc caccgccttt tcgatcgccg actgcgcccc
    14941 gccaagcgag caggcgccga tgttgagccg gccaccgtca agtccggcca tggcgatctt
    15001 gaagccctgg ccctcctcgc cgatcaggtt ggcgaccggg atgcggcact cctccatgat
    15061 cacctgccgc gtcggctggg catgccagcc catcttcttc tcgttcggcc cgaacgacag
    15121 gccgggcgtg cccttctcca ccagcacggc ggaaatccct ttcggccctt cagcaccggt
    15181 gcgcaccatg atgaggtaga gatcgcccgc accggcgccc gaaatgaact gctttgtgcc
    15241 gttgagtacg taatggtcac cctcgcgccg cgccgtggtg cgcagggccg ccgcgtccga
    15301 gcccgagcct ggctcggtga ggcaatagca ggacagcaga tccatcgtgg tcaggcgcgg
    15361 cagccaggtc tggcgcagcg tctcggagcc gtatttgtcg atcatccacg acaccatgtt
    15421 gtggatggaa atataagccg agaccgcagg acagccggtg gcgagctgct ccatgatcag
    15481 cgcggcctcc agccggccaa gaccggtgcc gccaacatcc tcgcgcacat agatgccggc
    15541 catgccgagg ccggccgctt gcttgatgac atcgatcggg aaatgcttct cctcgtccca
    15601 tttgagggcg aagggcgcaa tcttctcatc ggcaaagctg cgggccatgt cgcggatggc
    15661 gatctggtca tcattcaggg caaaggacat cgtggctccc atcgtaacgg cgggagtctt
    15721 gccgagagcc tgcgccgtgt cgagcgcaac cttcggcgat tcccgtcagc gccgccggag
    15781 acggcgcagg ttgtgacgca ggcgtttctc gatcggctgc accgtggcat gagcgatctt
    15841 cttctgcacc cccaacgccg ctcccatcat tgagcgcccg ttgagcaggt caaccgccac
    15901 tgtgaacggc gcggtcagtg ttgccagatg cgcgccaacg aagctttcgg tgaacgcctc
    15961 gaccttttcc gtcaccgcca gccggctttc gtcgctgacc agcggattgg gatgcagcgc
    16021 ctcgaaccac agctgcggca ggcggttggc gatcaccagc ggcgtcagga acaaggcgct
    16081 catcgtcatg ccctcaaagg ggacggattc gtccgtcccc ggagccagtc tcttagcagt
    16141 cttgtcccgc cggatcatcc cctacctatt gcatggtggg gatgacaaaa tcggcgccgt
    16201 ccttgattcc cgatggccag cgtgccgtca ccgtcttggt cttggtatag aagcggaacg
    16261 catccggccc atgctggttg agatcgccga agccggaccg cttccagccg ccgaaggtgt
    16321 aataggccag cggcaccggg atcggcacgt tgatgccgac catgccgacg ttgatgcggg
    16381 cagcgaaatc gcgtgccgca tcgccgtcgc gggtgaagat cgccgtgccg ttgccgtatt
    16441 cgtgatcatt gatcatcttg accgcaccgg cataatccct ggcgcgcacc accgacagca
    16501 ccgggccgaa gatctcttcc ttgtagatgc gcatgtcggt cgtcacatga tcgaacaggc
    16561 agccgccgag atagaaaccc ttctcgtagc cctgcatctt gaagccgcgc ccgtcgacga
    16621 caagtttggc gccttccttg acgccgagat cgacatagcc cttcaccttg tcgcggtggg
    16681 cggcggtgac cagcgggccg aaatcagccg aaccatcggt cgaggggcca accttgaggc
    16741 tttcgacgcg cgggatcagg cgcctgacca gttcgttggc ggttttctcg cccaccggca
    16801 cggccaccga gatcgccatg cagcgctcgc ccgccgcgcc gtaaccggct ccgaccagcg
    16861 catccaccgc ctgatccatg tcggcgtcgg gcatgatgat catatggttc ttggcgccgc
    16921 cgaagcactg cacgcgcttg ccgttggcgc agccgcgcga atagatgtat tcggcgatcg
    16981 gcgtggagcc gacaaagccg atcgccttga tgtcggggtg atcaaggatg gcatcgaccg
    17041 cttccttgtc gccgttgacc acgttgagga tgccctgtgg cagacccgcc tcgatggcca
    17101 gttcggccag catcatcggc acgcccggat cacgctcgga gggcttgagg atgaaggcat
    17161 tgccacaggc gatcgccggg gccagcttcc acagcgggat catcgccggg aagttgaacg
    17221 gggtgatgcc gcagaccacg ccgagcggct ggcgcatcga atagatgtcg atgccaggcc
    17281 cggcgccatc ggtgaactcg cccttgagaa aatgtggcgc gccgaggcag acctcgacca
    17341 cctcgacgcc gcgctggatg tcacccttgg cgtcgggaat ggtcttgcca tgctcgcggg
    17401 ccagacaatc ggccagcttg tcattgtcgc gggcgatcag ttcgaggaat ttcatcagca
    17461 cacgcacccg gcgctgcgga ttggtcgcgg cccatgcggg ctgcgccgcc gctgcatcgg
    17521 ccaccgccgc atcaagatcg gccttcgagg cgagtgcgac cttgccccgg acactcccgt
    17581 ccaatggctg gaaaacctcg gaaaagcggc ctgacgtgcc aggcacatgc ttgccgccga
    17641 tgaaatggcc ataggtgatc atgaacagcc tcctgcgttg gcgtgaccct aggtcttgtc
    17701 ttttctcaca tcaaggcaga agatcgcgca tccgttgtgc agaaaatagg gtccaccttg
    17761 gattgggatc atgcccgcgt gtttctggcc gtggcccggg ccggccagtt tctggccgca
    17821 gcgcgccatc tgaggctgga ccatgcaacg gtcggacgtc gcatcggggc gctcgaggac
    17881 gggatcggcg cacgattgtt cgagcggcgc accaacggct gcgtgctgac accggcgggg
    17941 gagaagttcc tgatcgccgc cgagcgggtc gaggccgaga tgctgaaggc gcaggccgat
    18001 ctttcggaag cggatgtcgc cgtcgccggc acggtgcgga tcggtgcgcc ggacgggttt
    18061 ggaacgctgt tcctgtccgg tcgtctcggc cggctgatgg tccggcatcc gcgtctgacc
    18121 atccagttgg tgccgctgcc gcgcaccttt tcgctttcca aacgcgaggc ggacatcgcc
    18181 atcgtcatcg accgaccgga agagggacgg ctgcatgtgc gcaagctgac cgattatacg
    18241 ctcggcttct atgcggcagc gacctatctc tgcgaacatg aggagccccg ttcgatcgag
    18301 gccctgaccg gtcaccgact ggtcacctat gttcaggacc tgcaatttgc cggagcgctg
    18361 aattatttcc cgcaagggtt cgagcccagg ttccagcggt tcgaatgcgc cggcgtcctg
    18421 ggccagttcg aggcggttcg ctccggtgcc ggcatcggaa tcctgcatga ttaccacgca
    18481 cgctccgatc catcgttgcg gccggtcctg cccgatcaca ggatccagcg gacctattgg
    18541 ctggtcaccc atgacgacac ccgctcgctg gcgcgtgtgc gggcggtggc cgagttcatc
    18601 ggcgatgaag tggccggtgc ggcgcgggca ttcctgccac aatagacctc gaccatgttt
    18661 tggccggatt ttgggatcag gggagggccg gcttaaccct cccttcaagg ctgtgacgta
    18721 tcggaccggc ttgccctgct gatctgcact atccactcat gacccttatg cattcgcgcc
    18781 gcgccgccct gtccagtctc gttgctctgc cgcttgccgg atgctgggct ggcccttatt
    18841 cgtcgcacga gggagcggct ctggccgccc gaaccaatga tgcacccgtc ctgcatgtga
    18901 tcaccagccg ccgactggcg ggcagcggag cgcagccacc ctatttcgat tggcgcaggg
    18961 ccgccgaact gacctatgcc gaagcccgct atgcccggcc cgaccgctcg gtgcttgggc
    19021 gcatcggctc ggtcgtctca ggcgattttg gcatgtccga ggtcagtccg ttcgccggcg
    19081 aagccctcga ccgcctcgcc gaggtgctga gcagtcgcga cagcctgctg ttcgtgcacg
    19141 gttataacca gaccttcgaa gcggcggcgc gcgacgccgc gcggctcggc gatgacatcg
    19201 gcttcgacgg gcggacgatt ctattcacct ggccctcgaa aggcggcctg ttcgactacg
    19261 gctatgatcg cgaatcggcg atgctggcgc gggaccatct tgccgatacg ctgcatgcgc
    19321 tgctgggcga gggacggtct ggcagggttc acctcgttgc tcattcgatg ggcacgctgg
    19381 caacgctcga agccttgcga ggctatcgcg accggcatgg cgaggccggg ctggagcgtc
    19441 ttggaacgat cgtgctggcg gcgcctgaca ttgatttcga cgtgttcaag gccggcctgt
    19501 cgcggctggg gccgctgcgt cagaaactga ccgtcatcac tgcgaccaat gaccgcgccc
    19561 tggacctgtc ccgccgtctg gccggtggtg accgcgtcgg cgccctttcg ggcgaacagc
    19621 ttgtcgggct cggcgtgcgt attgtcgatg ccactgattt tgcttcaggg ctgacgcggc
    19681 atgatgcctt catctccaat gacgacgtcc ggtcggttat ccgccgcgcg atcgaaagaa
    19741 gctgacaatc ccgcagtcag ccgcaagcat gtgcggcccg atacagcaga ttgttaaccc
    19801 taacgctgca tgaatacgac agttgttcta acggatctgt ctgtatgcgc gcgaaatctt
    19861 acctagcagg aattcttagc gggcttggtt ttgtttgctc cgcttatgca agcgatgctc
    19921 cttccccggc acctctgaca cccgacattg gcctgcgctc ctacattgac attgcgccgg
    19981 actattcaga ccgcattctc gactggcagt gggatgtcaa cgacaaggcc gtccagccgc
    20041 tggaagcgat caagaacggg cagcttgcgc cgggcaagct ctatgtcggc ggcctggtgc
    20101 gctggtccta ttccaatgaa cattccaaca ccttgggcaa gtttccgttc cttggccagt
    20161 ttcctgacca gcacaagaag cagttccgca tcggcgagac gatgttcaac aacacccacc
    20221 tcggcctgac actggtgccg gcgcactgga tcgccggctc tgccgaggtc atctacaccg
    20281 acaagaccta tccaggtcag gacaacgtgc agatccgcaa ggcattcatc tcggtcggcg
    20341 atctgaaaac cttcccgatc tatgcctcgt tcggcaagaa ttccgtcgat ttcggcgata
    20401 tgcgttcatt caacccgttc gtccattcga tgacgaacca ttatttcaac gtgcatagcg
    20461 aagacgcggt cggcatggtc ggctatttcc gcgacggctg gcacgttact gcatccgcca
    20521 tcggcggcgg ccggcagctg cgggttgccg acagccccag caagcgcggt tcgatcggaa
    20581 attttgccat caatggctcc aagaccttcg agttcgcgga tggattcagg gtcaagctgg
    20641 gcggcggcta tatcgacaac accatctaca acacgccgac accgcaccac acctcgcggc
    20701 aggtcgcagc ttccggcgcg cgcgttcaca acggcgcctg ggatgtgaac ggcatggtca
    20761 gctacaaggg cttcgacgtt cagaccgaat atacctcgac cgtcggccga tggcccgcaa
    20821 ccgaccatcc cgtctcgacc ttgaccgtgc agggccgcta cacgaccgac ctctggggct
    20881 ggcagaccat catttcagcc gggttcagcc gcggtgaaca gggcgccgac aacacccgct
    20941 ggcagcagat gcgccagatc gttgccggtc tcgagatcaa gccgcacaaa cagctgtcgt
    21001 tgctgatgga atacgcccat ctctccggct tcgttccgct ggtggcgatc aagagcgtgg
    21061 cagatccggg ggtccgctcg gatgtctggg tctttggcgc gcgcgcccat ttctgatcgg
    21121 gatgtgacgc aaggcaggcc tcaatggccg gaatgccgcc ggcttcggct cttgcgaggg
    21181 gggcacggtc gacattgccg cttgtcggtc ctcttgtgag acgttaccat caggcagttc
    21241 ctgaaatgac gagttgttcg tgacgtctag gccggagtcg ccattcaagt tcgaaaaagc
    21301 cgacatcaag atgtcggtgg acaagggcga gtttcgcctt cagctcaagg ggccggtcat
    21361 caacctcaag ctgaagaagc cggacttcaa gctcaagaag cccgatatca agcttgatga
    21421 cgatacgcgc tttgtccttt catggctggg cagcccgctg aagaccggtg ccgtttcgcc
    21481 gtctggcaag gagctggcac gtgcgatggc cgaaccgatt gacccggcga ttccaggttc
    21541 gatcattgaa ctcgggccgg gaaccgggcc ggtgaccgag gccttgctgg cgcgcggcat
    21601 tgccccggag cggctgatcc tgatcgaata caataccgac ttctgcaagt tgctgcgcca
    21661 gcggttcccc ggtgtgcagg tcattcaggg ggacgcctac aatctggccg cgacactggc
    21721 tggccaggtc gagaataagg ttgccgccgt tgtctcctca ctgccgttga tgacgcggcc
    21781 ggatccggaa tgcgcccgac tggcccgtga ggcgtttgcg atgatggcac ccgatgcgcc
    21841 gttcatccag ttcacctatg gcccgacctc gccggttccg ctcaagggac agccgttccg
    21901 tggccgtgcg gtcaagcggg tctggaaaaa cctgcctccg gcccgtgtct gggtttaccg
    21961 ggcgatgtga ccgacacgac tatttttgca ttcacgcaaa ataaatgatg gggccttgca
    22021 attgcgccat gctggtcaaa ttgccggacc cgtcctactt ctgggtttga attgccaatc
    22081 gcgaggggtc tagcatgagc gtcaagatac tggtgttttc gggttcatcc cgcgccggct
    22141 cggtgaatga tcgactggcc ggcattgtcg cccgccagtt gcagggcatg ggcgcggagg
    22201 tgacctacat caacctcggc aagttcgacc ttcccatggt caacgcagat gggtatggca
    22261 gcgctgcccc aaaggcagcg catgacctga gggcgatcat cgacgagcat gatgggttgt
    22321 tcatcgtctc accggaatat aatgccggct atacacctct cttgaagaac gcgcttgact
    22381 gggcatcggt ggccaagccc ggcgcgcccg cgtctggcct ttcgggcaag gtggtggcgc
    22441 ttggcgcggt ttcgccagga ccgatgggcg gctatcggtc tttgacccag ctgcgcacgg
    22501 tgctcgaact cggcttcggc gcgacgctgg ttccggagat ggtggcggtt ggcaacgccg
    22561 gttcggcctt tgccgaatca ggcggcctgg ctgatgagcg gaccgccggc ttcctcaacg
    22621 cgacgctgag caaactgatc acgatgacca agaagctcaa ggcgtgatcg gcacgcatca
    22681 cctctatctg acccacccgc aggtcaccat agacccggca gttccggtgc cgctctgggg
    22741 gctgtcggct gtcgggcgcg cacgaatgga acggctggca ggcgagggct ggatccgtcg
    22801 atttggtcgt atcgtgtccg ctgacgagac caaggcgctg gaggcggcag cgattctggc
    22861 ggcagcgctc ggctgtcagg ttgaacagcg gggcggcctg catgagaacg accgcagcgc
    22921 caccggtttt ctgccgccgc cggaatttga ggcgacggcc gaccggttct ttgccgaccc
    22981 ggaccgcagc attcgtggct gggagcgggc cgtcgatgcc cagcagcgca tcgttacggg
    23041 cgtgcgcgcc atcatcggcg agcatccgga tctgccgacg atcttcgttg gccatggcgg
    23101 ggtcggaacc ttgctccgat gcgcgctgcg gggaatagct atttcgcgca aggaggatca
    23161 gggcccgggc ggtgggggct gccatttcgc atttgcgctt gagccggttt cggtgttcta
    23221 cccttggcga ccgctcgaga ttccgccagc ctgaaggagg gcgtcatgcc tgtcccgcca
    23281 agagaacgcc tgattgtcgc gcttgacctg cccggtgtcg agccggcgcg ggccatggtg
    23341 gcccggctgg gtgacaaggt cagtttctac aagatcggca aggaactggt ctatgcgggc
    23401 gggctcgatc tggcgcgtga cctcgtcgca gccggcaaga aggtttttct cgatctcaag
    23461 ctgcatgaca ttcccgccac ggtcgaacgg gccacggcgg cggtttcgga tctgggcgcc
    23521 acctatctga ccgttcacgc ctatccgcag acgctggccg cagccgttag gggcaagggc
    23581 gacagcaatc tgcgtattct cggcgtttcg gtgctcacct cgatgaacga tgatgatctg
    23641 gccaaggccg gctacggtct cggtgtcagc gatctggtcc gccgccgggc acgtcaggcc
    23701 tatgaggcgg gcgcggcggg gttgatctgt tcgccgctcg atatcgcgat tgtccgtgac
    23761 agcgtcggcg atggcctgga aatcatcacc cccggcgtgc gcccggccgg cagcgatcat
    23821 ggcgaccaga agcgcgtgct tacgccgggt gaagcgatcc gggccggtgc cgaccggctg
    23881 gtggtcggtc ggccgatcac cggagccgct gatcctgccg ctgcggcggc tgcgatcgtc
    23941 gccgagattg cgggcgcggc agcctgaccc gtccggcgtt gaccgggacg ccgctccgtg
    24001 gcctagatca cgctctcgtt ttgatgggct catcaaagcg agagataacg tgatcgatta
    24061 caatagttta gagcatgctt tccgcgaaag accgttttca ctttttcgca gcatgctcta
    24121 acagacgaca tcaggagaca gcgcatgacc aagggctact ggattgcccg cgtcgatgtt
    24181 tacgatgccg aggcttacaa gaactacgtc gcccataatt cggcggcctt caagaaatac
    24241 ggcgcccgct ttctggtgcg cggcggccag ttcgcgctga aggaaggcat ggcgcgctcg
    24301 cgcaacgtgg tgatcgagtt tccctcttac caggcggcgc tcgattgctt tcactcgcct
    24361 gaataccagc atgcgctcag cttccggcgc gggccggtgt cggcgggcga tgtggtcatc
    24421 atcgaaggct atgacgggcc gcaaccggga taggcaacca tgtctgattt gaaactcgcg
    24481 gtggtcggcg ccgccggacg catgggccgc atgctggtgc gcgcggccat cgagcccgac
    24541 ggggtggccc tggccggtgc gctggagc
//
LOCUS       DAOMXG010000013        24495 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_1272_length_24495_cov_12.733429, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000013 DAOMXG010000000
VERSION     DAOMXG010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 24495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24495
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1272_length_24495_cov_12.733429"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="PLQ11_01865"
     CDS             complement(<1..391)
                     /locus_tag="PLQ11_01865"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HRK23677.1"
                     /translation="MAWTREQMAARAAKELRDGFYVNLGIGIPTLVSNYIPAGMSVQL
                     QSENGMMGMGPFPYEGEEDPDLINAGKQTVSELPTTSYFSSADSFAMIRGGHINLSIL
                     GAMQVAENGDLANWMIPGKMVKGMGGAM"
     gene            complement(391..1122)
                     /locus_tag="PLQ11_01870"
     CDS             complement(391..1122)
                     /locus_tag="PLQ11_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HRK23678.1"
                     /translation="MRKVFPDAKSALDGICRDGMTIMAGGFGLCGMPEDLIIALRDTG
                     TKGITAISNNAGVDGKGLSLLLETRQIRKMISSYVGENKLFAAQYLAGELELEFNPQG
                     TLAERIRAGGAGIPAFYTKTGYGTIIADGKPTAEFDGEHYVMERWLRADVSLVHAYMA
                     DTEGNLVFRKTARNFNPMMATAGKVTVVQCEHLVRAGDINPDHIHTPGVFVQRIVHTP
                     NAAKYIENRTTRKHTSAPAGTGGDV"
     gene            1322..1774
                     /locus_tag="PLQ11_01875"
     CDS             1322..1774
                     /locus_tag="PLQ11_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23679.1"
                     /translation="MHLASARRALALGLVLTLPAVAQDCMTMGAMTMCRNGFMSFGLG
                     VPQPLPGGGFSMTLDQTAPGGTMMFHSFGLPPAGGTFVSTQAMVAPDGTRTQMTLRET
                     ANPDGSRSRRVETRVTASNGQVRETVEDWIIHPDGRACRVDAGKPACE"
     gene            1885..2829
                     /locus_tag="PLQ11_01880"
     CDS             1885..2829
                     /locus_tag="PLQ11_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HRK23680.1"
                     /translation="MQLYDAGVVQAALNWPDLIAAIEQHFAAPVEVPQRQVLTIANPK
                     GENGVLLLMPAWLGGDSIGVKAVTFFPGNTAKGISTISAAYLLFDGETGAIRAAMDGD
                     AITVRRTAATSAAAAKRLARTDARRLLVIGTGQLSEHMASAHAAVRVYESIEIHGRDP
                     AKASAVAGALRRSGLAASVALDLEASVCRADVISCCTSASTPLFPGAWLQPGCHVDLV
                     GSFKDDMREVDDEAVRRSAIFVDAKPGALLSGDLAQPLRDGVINEGHILADLADLVSG
                     RHPGRGNAAMITLFKSVGNAREDLAAARLIAETRKVPG"
     gene            2848..3099
                     /locus_tag="PLQ11_01885"
     CDS             2848..3099
                     /locus_tag="PLQ11_01885"
                     /inference="COORDINATES: protein motif:HMM:NF016828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HRK23681.1"
                     /translation="MTGRSLLKSGGINCIFGSCWHHGPIGRRTMKKIIQQFIENRSGA
                     TAIEYALICALVFLAIITAVQTYTNSANKMYEKISNTMK"
     gene            complement(3205..3627)
                     /locus_tag="PLQ11_01890"
     CDS             complement(3205..3627)
                     /locus_tag="PLQ11_01890"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006335425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRK23682.1"
                     /translation="MKKILAVLVMAWAMLFSASLAQAKVVAKISVAQQKMTVSVDGEV
                     VHVWNVSTARKGFVTPRGTYAPKRLHARYYSKKYYNSPMPYSIFFKGGYAVHGTSAVG
                     KLGAPASHGCVRLATGNAAKLFALVKAYGPAKAKIVIT"
     gene            3829..4788
                     /gene="mepA"
                     /locus_tag="PLQ11_01895"
     CDS             3829..4788
                     /gene="mepA"
                     /locus_tag="PLQ11_01895"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018045601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HRK23683.1"
                     /translation="MIRRLSIARGLATASLVSAGFWLALAAGTAAQETYTEEMATRAK
                     LLQTLPADAAKRHFGSAKAAVPGPARTIGSYAKGCIAGARALATNGPTWQVMRLSRNR
                     NWGHPDLVALIERIARKAPSAADWPGLLVGDLSQPRGGPMLTGHASHQVGLDADIWLT
                     PMPERTLSSSERENMSATNVVASDWMDVDPARWTKAHVGIIRLAAEQPAVARIFVNPA
                     IKKALCRDAGNERAWLSKVRPMWGHNYHFHIRIGCPEGDSACQDQDPPPSGDGCGKEV
                     DDWLAMQHRAYFGPKDPNAKPKPPVIVPLSAMQEACQQIVLQK"
     gene            4785..5915
                     /locus_tag="PLQ11_01900"
     CDS             4785..5915
                     /locus_tag="PLQ11_01900"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRK23684.1"
                     /translation="MTHTGQPTELLSATPRPALTSALIMTIGVIFAFEVLVPVAPGDN
                     WYSPSTATLMAYGGMSGHLVLEKGEVHRLLTAPLLHAGPLHLAFNALALKIAGDVLER
                     MLGRWWLGALLILGALAGSLASVLVNAPNVIGVGASGAIMALFGCFLAMAFRFPPGPV
                     RRSFLGNGLGTLLPSLLPGLLPMVTGGEGFAIDYAAHGGGAALGLVFGGLLLWLWPAD
                     HPHPGGRGFAKAVTVLGLAGIVFAVVQLFGSLSLYRYVGAMIPEQAIPKTDAEARAAG
                     PHLSERYPLDPRSHFYRALDRIAAKDLAGAETALQEAVRLADAWPQVFPADFRVRLHM
                     VQALVLADRGKLETARAVARPACTAQVDDSIRGPLRRAGLCR"
     gene            complement(5916..6461)
                     /locus_tag="PLQ11_01905"
     CDS             complement(5916..6461)
                     /locus_tag="PLQ11_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HRK23685.1"
                     /translation="MLERAVERFLFASRWLLAPFYIGLVLALCALLVKFLQEVWHGIT
                     HLITATEADVILMTLALVDLTFTASLIVIVIFSGYENFVSRIDAGAHADWPEWMGKID
                     FSGLKLKLMSSIVAISAIQLLKAFMNIRNVGEREVLWLVIIHLVFVVSGLLMAWTDRL
                     GEHHASPSAKVSADDSKGADH"
     gene            6665..7627
                     /locus_tag="PLQ11_01910"
     CDS             6665..7627
                     /locus_tag="PLQ11_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HRK23686.1"
                     /translation="MAKLVTVFGGSGFVGRHLVRALAQRGWRVRVAVRRPDLAGFLQP
                     LGGVGQVQAVQANVRNPASVAHAVAGADAVVNLVGILSESGRNTFGAVQAAGARTVAE
                     AVAAAGVRSFVHMSALSANASSVSDYAASKAYGEQAALKAVPNAVIMRASVIFGPEDK
                     FFNRFASIARMSPVIPLIGGGETRFQPVFVGDVAEAIAVALEGKAKPGMIYELGGPEV
                     ASFKTLMEYVLKETGRTRMPVKLPFWAARMQASVLQILPKAPLTVDQVNLLEYDSIVS
                     PEAAREGRTLAGLGISPTTYESVVPSYLYRFREHGQFERSRAAS"
     gene            complement(7655..8647)
                     /locus_tag="PLQ11_01915"
     CDS             complement(7655..8647)
                     /locus_tag="PLQ11_01915"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HRK23687.1"
                     /translation="MTKQRPVVIVTRRLPEVIEQRMAELFDVRFNQADTPMSEAELVE
                     AMNACDVLLPTVTDRIDEALVARAGPRLKLIANFGNGVDNIDVAAARKRGITVTNTPG
                     VLTEDMADFTMALIVAVARRFSEGARIIPGGHWTGWSPNWMLGRRIHGKRLGIVGMGR
                     IGLAVARRARAFGLQVHYHNRRPVSARTEEELQATYWESLDRMVARMDIITIHCPHTP
                     ATFHLMSARRLKLLKPDAILVNTARGEIVDSEELTDLLESGHLAGAGLDVYEHEPAIS
                     DRLVRLADQGRVLLLPHMGSATFESRIEMGEKVIINIKTFTDGHTPPDRVLPQL"
     gene            8823..9371
                     /locus_tag="PLQ11_01920"
     CDS             8823..9371
                     /locus_tag="PLQ11_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRK23688.1"
                     /translation="MWVAMKTCSIRSRMVCALVPVGLGLAAGPGLAAEQITTGTATGL
                     PIPRYVSLKPSDTPMREGPSKDHRIKWVFKREGLPVEITAEFETWRRVRDAEGTEGWV
                     FHSRLSSRRTALVAPWSKEANLPLHDEGSETSGIVARLQPTVLANVETCTGKWCRING
                     EGFRGWMKQDQLWGVYPGESVK"
     gene            complement(9392..9856)
                     /locus_tag="PLQ11_01925"
     CDS             complement(9392..9856)
                     /locus_tag="PLQ11_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019905806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HRK23689.1"
                     /translation="MTMQDTPIKPSSGSSRSGCPVHDLKGMLRTAGLRPTRQRVALGW
                     LLFGKGDRHVTAESLHEEAQKARVPVSLATVYNTLHQFTGAGFLREVAVEGAKAYFDT
                     NTGNHFHFYVEGEGRLIDIANADIEMRQLPEPPAGMEIAGIDVVLRLRPRAG"
     gene            10002..10535
                     /gene="fabA"
                     /locus_tag="PLQ11_01930"
     CDS             10002..10535
                     /gene="fabA"
                     /locus_tag="PLQ11_01930"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013527885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     3-hydroxyacyl-[acyl-carrier-protein] dehydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     FabA"
                     /protein_id="HRK23690.1"
                     /translation="MSQQAQTGRAKSSYTYDDLLACGRSELFGPGNAQLPLPPMLMFD
                     RITDISETGGAFDKGYIRAEFDIRPDHWFFPCHFHGNPIMPGCLGLDAMWQLTGFFLG
                     WLGEPGYGMALSTGEVKFKGMVTPKTRLVEYGIDFKRVMRGRLVLGIADGWLKADGET
                     IYHATDLKVGLSKQKAG"
     gene            10569..11789
                     /gene="fabB"
                     /locus_tag="PLQ11_01935"
     CDS             10569..11789
                     /gene="fabB"
                     /locus_tag="PLQ11_01935"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003604005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HRK23691.1"
                     /translation="MRRVVVTGMGIVSSIGNNVQEVTASLHEAKSGITYAPDFEEHGF
                     RSRVYGRPTLDPAQVVDRRAMRFHGGGSGWNHVAMDEAIRDAGLGEAEISNVRTGIVM
                     GSGGPSTRTVIEAVDKARESGNSKRVGPFAVPKAMSSTASATLATWFKIKGVNYSISS
                     ACATSNHCIGNAYELIQWGKQDRVFAGGCEELDWTLSVLFDAMGAMSSTYNNSTASRA
                     YDKNRDGFVIAGGAGVLVLEDMEVAKARGAKIYAEIVGYGATSDGYDMVAPSGEGAVR
                     CMQMALGSLKAPIDYINPHGTSTPVGDIKEIEAVREVFGARAQAIPISATKSLTGHSL
                     GATGVQEAIYSLIMMKNGFVCESAHIEEIDPDMADMNIVRKRIDNARLGHVMSNSFGF
                     GGTNATIILKHPDA"
     gene            11896..12690
                     /gene="fabI"
                     /locus_tag="PLQ11_01940"
     CDS             11896..12690
                     /gene="fabI"
                     /locus_tag="PLQ11_01940"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HRK23692.1"
                     /translation="MLMQNKRGLIMGVANSNSIAWGIAEMLSRHGAELAFTYQGEALG
                     KRVRPLAEGIGARLVLPCDVEDISSVDSLFETLDREWGSLDFIVHAIGFSDKNELRGR
                     YADTSRDNFVRTMVISCFSFTEIAKRAAALMPNGGSMITLTYGGSVRVMPNYNVMGVA
                     KAALEASVRYLAADLGPQGIRINALSPGPVRTLAGAGIADARSMFSFQQKHAPLRKSV
                     SLQEIGGSALYFLSDLSGGVTGEVHHIDAGYNIISMPRREDMEKPE"
     gene            complement(12782..13543)
                     /locus_tag="PLQ11_01945"
     CDS             complement(12782..13543)
                     /locus_tag="PLQ11_01945"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRK23693.1"
                     /translation="MIDLLPPGISPFVALALVAVSFATSALTAAFGIGGGVAMLGALA
                     STVEPTMVVAVHAVVQIGSNIGRTVLQRVHVVWPIAARFTFGSIFGIALGATLFVALP
                     VRVLLALIGVFILAMTWVPKPGIPGLERSGVVLGGAVSSVITMFVGATGPFINAMLKV
                     LGLERRQMVATQAMCMTIQHTLKAVAFGLIGFSFGQWVPLMAAMIASGFAGTWLGTQL
                     LERMSERFFSIVLKTLLTLIALDLLRQSLGVPLPF"
     gene            complement(13866..15275)
                     /locus_tag="PLQ11_01950"
     CDS             complement(13866..15275)
                     /locus_tag="PLQ11_01950"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRK23694.1"
                     /translation="MATRYEADTARRTELPVPPRIAKPILLIEDDATLNRLIISQLSR
                     AGYNLKGVLRAQDAKRAIEEEEPALILLDIRLPDRDGLDFLQELQHICPVIVLTAHGA
                     IHQAVRALKLGAHDYLTKPIRPEELELAITRALDHAQLKRSGDFLKDRLQSLVATTMV
                     GTSKVFDEVRRQIALFGPTDATVLILGESGAGKELVARSIHESSARAKDAYVAVDCST
                     LHENLFESELFGHERGAFTGADRRKLGLVEVAEGGTVFLDEIGELSLGLQAKLLRVLE
                     LGTYRRVGGVTDLKANVRFIAATNRDLVAMGAQNRFRTDLYYRLSRFVMNVPALRDRG
                     DDAVQLARHFVRTRNFHRGVDKVLDDSAVAAIRTYQWPGNVRELRNVVERAVLLSGEE
                     SAITAADLGLGIKAEPPAPDEGIRFTFKHLPTMDELQAQYIEMLLERRHFSRAKIAEA
                     LGISERSVYRILQTLKTRG"
     gene            complement(15259..17154)
                     /locus_tag="PLQ11_01955"
     CDS             complement(15259..17154)
                     /locus_tag="PLQ11_01955"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRK23695.1"
                     /translation="MRSTSLFAILTRSLLFYSLLVALLFIGGFAWVYQNQIKDQRAEA
                     ATRLGLLLQVSLENAMLKRDIPGLKDIIDRLGRQDGIASVMILNPQGEVRFASDPRLL
                     DRSFSFASGALCPGCAWDGKSLLERSEILQSGPLSDMPVLRSVKAVANREACSQCHGN
                     PQLTPFNGVLVVDYHAQDVQKKALTGALQLVGAGLLIVLGLLAGTYHALHRHVLAPVA
                     SLAEASRALAGGDLTRRVHGASQTELGDLGRTFNDMAERLARSISELAARERFVQALL
                     DAMPDGIRVIDSDYRIVHANRTYREHVGSTSGSEVGRFCYQSSGNRTEPCVPTLVTCP
                     LVALTDTNSVLKYQSHHTRPDGGVIHVEINAARVRLEGPDGNRFLVIEVIRDLDAEMR
                     ISHEQRLSEIGQLATGVAHEIRNPLSSVAMLLANTEANLRRGEAAEAEMAMRLIGHEI
                     DRCLKITDSLLKLGTPPGSEPQLISLNEVVADIVSLLRYQAEDGHVVMSSDLTPDLRV
                     LGSDSDLRIVLINLIQNAFHSMPQGGQLTLVGRRGARQTVSLSVSDTGVGIAEEHLNN
                     IFLPFWSRRADGVNGTGLGLAICRSVISHLGGTITVTSTLGVGSTFTMTLPDPDASQE
                     AQHGHAL"
     gene            complement(17156..17614)
                     /locus_tag="PLQ11_01960"
     CDS             complement(17156..17614)
                     /locus_tag="PLQ11_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23696.1"
                     /translation="MTYWCQRVIDAIGRLGPGGFVVDFASYRPDLVQDMAAALQCAHV
                     DFRRLVMAPLGSNAHTVPLTAIEATIAGQANERGIVLHNAEALLATRSASDRRSFVEG
                     FLAAPRSNVVVLPMALFGREIADHERIVRLTQEDLPTESLLVQLASMRFQ"
     gene            complement(17611..18615)
                     /locus_tag="PLQ11_01965"
     CDS             complement(17611..18615)
                     /locus_tag="PLQ11_01965"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRK23697.1"
                     /translation="MNPLGSCFPEIDADRCVHALAPFASCRACVEICPTGAWLLDEEG
                     LALATDRCTSCGQCVPACPREALQMPRPMAILSRAKDQSDAFAACQRSGVPGATLACV
                     HAIGLRDLDRMADDGINSLTIATGACATCDRAPKGPDLEGAAARHARLRASRRQPVTT
                     LRRLDADAYSRALARAKEDVDPVDRSRRRLFSAFVDRPPAVVATVPPEPAEPRAALRP
                     QIDSARCVACDACIRACPDGAMLSVATETGPAYVIEPLACTGCRLCIDVCDQHAVSLD
                     RDGLAASQQIGLDPYRCRACGVRYYALSGVAAPDAGGLCRICARTGHFKKLYQVFKDA
                     "
     gene            complement(18615..19394)
                     /locus_tag="PLQ11_01970"
     CDS             complement(18615..19394)
                     /locus_tag="PLQ11_01970"
                     /inference="COORDINATES: protein motif:HMM:NF014651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HRK23698.1"
                     /translation="MNIGLRDHPGQPGGNDHREILGLMAEDLLLLAALHDREVSAPLL
                     GELRRGPLAERLFLGPGDAAGESAFAVFDAALASDECDGTSQHMDELAADFAAIYLNN
                     SYSLSPLESTWVDPEGLIMQEAMFEIRRWYHHYGVAAGDWRKRPDDHLVHQLEFIAHL
                     LTHPVEHAPKDAAAFIDHHILRWIEPFSMRVAQRCEKQFYAGLALVTAAVLHRLRDVL
                     VDVADMPLIELEPIEEEKRRRREEAQKQASERYLPGAAPSW"
     gene            complement(19391..20056)
                     /locus_tag="PLQ11_01975"
     CDS             complement(19391..20056)
                     /locus_tag="PLQ11_01975"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRK23699.1"
                     /translation="MANETNKAAIKEAVKRKKREVYVPVKPDMQLGFIHHNVDCIGCR
                     ACEIACKDKNGLPPGPRFRRVQYIEGGTYPEVYAYKINMSCNHCAEPACLPTCPTGAI
                     FKRQKDGIVDIDSTLCIGCRRCEAACPFGAPQYNPETNTVMKCNMCVDEIDAGRKPYC
                     VMGCMMRVLDIGPIDKLRDGSYETKARGKNDEVVRQVKNMADPELTNPSIVFIPHSKG
                     RVG"
     gene            complement(20069..22618)
                     /locus_tag="PLQ11_01980"
     CDS             complement(20069..22618)
                     /locus_tag="PLQ11_01980"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRK23700.1"
                     /translation="MNDRLDPKRLNGALKLDRRRLLELAAVGGAAGLLPTAAPTPANA
                     FAYEPYPTDDQLETVVTSCAHNCGSRHALIAHKKGDVIVRLSTDDGRYQKDGFFGKDT
                     EEEPQVRACLRGRSYRARLYSAERLLYPMMRVGERGEGKFKRVSWDEALTYVANKMME
                     LKAKYGPTAIVDQSYAGASYGVLHKSDQIEGLLGRFLGMFGCRTSSWSVPSYQGTTFA
                     SRMTFGTIEDGDEDDAFAHSKLIIMWGWNPAYTFHGGNTFYYMRLAKQRGCKFVLVDP
                     QYTDSAAAYDAWWIPIRPNTDAAMLAAMAHVIFTENLHNQAFINKFVLGMDAGTMPDW
                     AKDKENFKDYILGKYDKTPKTPKWAEAITGVKADDIIKLARMYATTKPAALKASWSPG
                     RNAYGEQYNRMASAVQAMTGNIGVLGGCSEGVGKGFKNDAVAYPYDEFANVWFAAIKS
                     DRWAHIVLNYPNVKREEVGLWPRGDQLDGVIPNIKGIFWQGSDWFNQLTNINKEIQAI
                     KKLELVVCMDSTITPSGLYADVLFPIATHFERHDVALPWYKGHYYIHRPKVIQPLGES
                     KTDFQVFTELAYRLGFGPAYNPKANRDYFLNDDNVDEAYLREWWEGKVMQNKYSKVTM
                     SWDEFKQRGIYKFLLTEPHVAFKQQIEKGVPFPTPSGKIEIFSTQLAQITDWKRTQYG
                     EEIPYLPKWIEPWESLNHPLTKKFPFHLVSPHPRWRTHSIFNNIPWLRETFSQEVTIN
                     ASDATKLGIKTGDTVELYNDRGRTVVPAYVTERCMPGVLVLHEGAWMDVDKDGVDRSG
                     NPDFLTDDNPSPAGAFAYNTVLCAIKKTDLVHKPGWDELATARSHVFRRDM"
     gene            complement(22630..23562)
                     /locus_tag="PLQ11_01985"
     CDS             complement(22630..23562)
                     /locus_tag="PLQ11_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23701.1"
                     /translation="MNRTYWMATALALSASIGAAEFLAPDGWRPGIASEAIAQAKPAA
                     TLPADRSDTMKKFDAETKKALAGDYETARYHPIHFPPNIASSTRSDCLTCHQEVLTTK
                     PRAESQAGAKANSVLAWYQTLDSYEGNQESFHWRHLESALAKQVMKLECNFCHQGSDP
                     REQSPVFTVPNALMTSNNGQAPFTNRKRVNPSETCLRCHGNFPYENMGLAGPWHEQRA
                     DLEPEGTPNGCMTCHAGIRTEKHNVTYLNKDGIDKASENSSDVCYGCHGGRSWYRISY
                     PYPRTPWPDMDKTVPDWAKNRPTKSESRYLISGK"
     gene            complement(23639..>24495)
                     /locus_tag="PLQ11_01990"
     CDS             complement(23639..>24495)
                     /locus_tag="PLQ11_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23702.1"
                     /translation="GMGGMSGMSGMGGMGGMSGMSGMSGMGGMSGMSGMGGMSGMSGM
                     GGMSGMSGMGGMSGMGGMGGMSGMSGMGGMGGMSGMGGMGGMSGMGGGNSWVCTQWSY
                     RPSMSGMSGMGGMSGMSGMGGMGGMGGMSGMGGMGGMSGMGGMGGMSGMSGMGGMSGM
                     SGMGGMSGMGGMSGMGGMSGMSGMGGMGGMGGMGGMSGMGGMSGMGGMSGMSGMAGAA
                     PGWVCTQWSSGAMRGMSGMSGMGGMSGMSGMGGMSGMSGMGGMSGMSGMGGMSGMSGM
                     GGMAGQKK"
BASE COUNT         4730 a         7604 c         7523 g         4638 t
ORIGIN      
        1 ccatcgcgcc gcccatgccc ttgaccatct tgccggggat catccagttg gcgagatcgc
       61 cattctcggc cacctgcatg gcgccgagga tcgacaggtt gatatgcccg ccgcggatca
      121 tcgcgaaact gtcggccgag gagaaatacg acgtcgtcgg cagttcggac accgtctgct
      181 tgccggcgtt gatcaggtcg gggtcttcct cgccctcgta agggaagggc cccatgccca
      241 tcatgccgtt ttcggactgg agctgcacgc tcattccggc cggaatgtaa tttgagacca
      301 gcgtcgggat gccgataccg aggttcacat agaagccgtc gcgcagctcc ttggcggcgc
      361 gtgccgccat ctgttcgcgt gtccaggcca tcagacgtct cctcctgtgc cggcaggggc
      421 cgaggtatgc ttccgcgtgg tgcggttttc gatgtacttg gccgcattcg gcgtatgcac
      481 gatgcgctgc acgaaaacgc ccggcgtatg gatatggtcg ggattgatat caccggcccg
      541 caccaggtgc tcgcattgca ccacggtcac cttgccggcg gttgccatca tcggattgaa
      601 gttccgggcg gtcttgcgga aaacaaggtt gccctcggtg tcggccatgt aggcatgaac
      661 gaggctgaca tcggcgcgca gccagcgctc catcacataa tgctcgccat cgaattccgc
      721 cgttggcttg ccatcggcga tgatcgtgcc gtagccggtc ttggtgtaga aggccgggat
      781 gccggcgccg ccggcgcgga tacgctcggc cagcgtgccc tgcgggttga actcaagctc
      841 aagttcaccg gccaggtact gggccgcgaa cagcttgttc tcgccgacat aagaggagat
      901 catcttgcgg atctgccggg tttccagcag cagcgacagg cccttgccgt cgacacccgc
      961 attgttggaa atggcggtga tgcccttggt gccggtatca cgcagcgcga tgatcagatc
     1021 ttccggcatg ccgcatagac cgaacccgcc ggccatgatc gtcatgccgt ccctgcagat
     1081 gccgtccagg gcggatttgg cgtcgggaaa aactttcctc atgaaagaga cgctccttgt
     1141 gtgaaagact tttcccgata gctcaggccg catggaatgg ccagagcaat gtccggtgcc
     1201 ccggccggtt aggaaccgtc agtcagcgaa aatgtcgcag gggattttag cccctagaca
     1261 acgcgccgcc ttgatggcgc cgcgcttgca tcaccatatc ggtcttgctc ggagaatgac
     1321 gatgcacctt gcttctgccc gccgtgccct agctcttggc ctggtcctga cgctgcctgc
     1381 tgtggcgcag gattgtatga cgatgggtgc catgaccatg tgccgcaacg gcttcatgtc
     1441 ctttggcctg ggcgtgccgc agcccctgcc gggcgggggc ttttccatga ctctggacca
     1501 gacggcaccg ggaggcacca tgatgttcca cagtttcggc ttgcctccgg caggcggcac
     1561 gttcgtttcc acgcaggcga tggtcgcgcc ggatggcacg cgcacccaga tgaccctgcg
     1621 tgaaaccgcc aaccccgacg gcagccgctc gcgccgtgtc gaaacgcggg tcacggccag
     1681 caatgggcag gttcgcgaaa cggtcgagga ctggatcatc catcccgacg gacgtgcctg
     1741 ccgcgttgat gccggcaagc cggcctgcga atagcacgca ggagcgcccg agccgttcat
     1801 tccgtagctt ggagacagga ttggcttgcg gcgcctgcgt ggtctaagac aggatcctct
     1861 tcaggagctg aacgggggat caggatgcaa ctgtatgatg cgggcgtggt gcaggccgca
     1921 ttgaactggc ctgatctgat cgccgccatc gagcagcatt tcgcagcccc tgtcgaggtg
     1981 ccgcaaagac aggttctgac gatcgccaat ccgaagggcg aaaacggtgt gctgctgctg
     2041 atgccagcct ggctgggcgg cgattcgatc ggcgtcaagg ccgtgacgtt ctttccaggc
     2101 aataccgcaa agggcatttc gacaatttcg gccgcctatc tgctgtttga tggcgaaacc
     2161 ggggcaatcc gtgccgcgat ggatggcgac gcgataacgg tgcggcgcac cgcagcaacc
     2221 tccgcagccg cagcaaagcg gcttgcccgc acggatgccc ggcgcctgct ggtcatcggc
     2281 accggacaat tgtcggaaca catggccagc gcccatgcag cggtcagggt ttatgaatcg
     2341 atcgagatcc acggccggga tccagccaag gcatcggcgg tggccggtgc cctgcgccgc
     2401 agcgggctgg cggcctcggt cgcccttgat ctggaagcct cggtgtgcag agccgacgtc
     2461 atttcctgct gcaccagcgc cagcacaccg ttgtttcccg gggcctggct gcaaccaggg
     2521 tgtcatgtcg accttgtcgg gtcgttcaag gatgacatgc gcgaagtgga tgacgaggca
     2581 gttcggcgtt cagccatttt cgtcgacgcc aaacccggcg cactgctctc gggcgatctc
     2641 gcccagccat tgcgcgacgg cgtgatcaat gaagggcaca tccttgccga tcttgccgac
     2701 cttgtgagtg gaaggcatcc cggccggggc aatgccgcga tgatcacgct gttcaagtcg
     2761 gtgggcaatg cccgcgagga cctcgccgcg gcccgtttga tcgcagagac ccgcaaggtg
     2821 cccggctgat accaaaggtc gccagcaatg accggccggt cattgctgaa gtcgggtggt
     2881 attaactgta tttttggcag ctgttggcat catggcccta tcggaaggcg gaccatgaaa
     2941 aaaattatcc agcagtttat cgagaaccga tcgggcgcga ctgcgatcga atacgcgctg
     3001 atctgcgcac ttgtgttcct ggccattatt acagctgtgc agacctatac gaacagtgcc
     3061 aacaagatgt atgagaagat ctccaatacc atgaagtgat acttccagcg gactgcgccc
     3121 ttgaagcgga ggccgaccag actggctcag atcgaacact ccaggctatt gatatagcat
     3181 cttctttttt accatcggca tttcctaggt aatgacgatc ttggccttcg ccggaccata
     3241 ggctttcacc agggcaaaga gtttggccgc gttgccggtc gccagtcgca cacacccatg
     3301 cgaggctgga gcgccgagct tgccgaccgc actggttccg tgaaccgcat agccgccctt
     3361 gaagaaaatc gaatagggca tcggtgaatt ataatatttc tttgagtaat aacgggcatg
     3421 caatcgcttc ggcgcataag tgccgcgcgg agtgacgaaa ccttttcgtg ctgtcgagac
     3481 attccataca tggacaactt cgccatcaac actgaccgtc atcttttgct gggcgacact
     3541 gatcttggcc accactttgg cctgagccag agacgcagag aacagcattg cccaggccat
     3601 taccaatacg gcaagaatct tcttcatcga cgccaacccc tgtttgtctg ttgcggctgt
     3661 gccggcccgg cttgaatgca aggctctgac gcgcagccct tcccgatctg tccgcataca
     3721 aaactgccag gcccgtttgt tcccggtaaa cggacgtggt ttataatcgg cttcggtagt
     3781 gccatggtaa tcagcctgtt tatcaagcct ttccagagag ttacgctgat gattcgacgt
     3841 ttatccatcg ctcgcggact ggcgacggca agtcttgtgt cggcaggatt ctggcttgca
     3901 ctggcggcgg gaacggcggc acaggagacc tacaccgagg aaatggcaac gcgggccaaa
     3961 ttgctgcaga ccctgccggc cgatgccgcc aagcgccatt tcggctcggc caaggcagcg
     4021 gttcccggcc cggcgcggac catcggttcc tatgccaagg gctgcatcgc cggggcgcgg
     4081 gcactcgcca ccaacgggcc gacctggcag gtgatgcggt tgtcgcgcaa ccgcaactgg
     4141 ggtcatcccg atctggttgc cctgatcgag cggatcgccc gcaaagcgcc gtctgcggct
     4201 gactggcccg gcctcctggt cggcgatctg tcacaaccgc gtggcgggcc gatgttgacc
     4261 gggcatgcct cgcatcaggt cggtcttgat gccgatatct ggctgacgcc gatgccggag
     4321 cgcaccctgt ccagcagcga gcgcgagaat atgtcggcga ccaatgtcgt cgccagcgac
     4381 tggatggatg tcgacccggc ccgctggacc aaggcgcatg tcggaatcat ccggctcgct
     4441 gccgaacagc cggccgtcgc ccgcatcttc gtcaatccag ccatcaagaa agcgctctgc
     4501 cgcgatgccg gcaacgagcg cgcctggctg tccaaggtca ggccgatgtg gggtcataac
     4561 tatcacttcc atatccgcat cggttgtccc gagggcgaca gcgcctgtca ggatcaggat
     4621 ccgccgccgt caggcgatgg ctgcggcaaa gaggtcgatg actggctggc gatgcagcat
     4681 cgcgcctatt tcggccccaa ggatcccaat gccaagccaa agccgccagt gatcgtgccg
     4741 ctctcggcca tgcaggaagc ctgtcagcag atcgtcttgc agaaatgacc cacacgggac
     4801 aaccgacaga gcttctttcc gcaacgcccc ggccggcgct gaccagcgcg ctgatcatga
     4861 cgatcggcgt aatattcgcg tttgaggtgc tggtgccggt ggctcccggc gacaactggt
     4921 attcgccttc gaccgccacg ctgatggcct atggcggcat gagcgggcac ctcgtcctgg
     4981 aaaaggggga agttcatcgc cttctcacgg cacctctgct gcatgccggc ccgctgcatc
     5041 ttgcgttcaa tgcgctggca ctgaagatcg ccggcgatgt gctggagcgg atgctcggtc
     5101 gatggtggct cggcgcattg ctgattctgg gggccctggc gggcagcctc gcctcggtgc
     5161 tggtcaatgc accgaatgtg atcggtgtcg gcgcctccgg tgcgatcatg gcgctgttcg
     5221 gttgttttct tgcaatggcg ttccgttttc cgcccggacc agtccgccgc tccttcctgg
     5281 ggaacgggtt gggcaccttg ctgccgtcgc tgctcccggg gttgctgccg atggtcaccg
     5341 gcggcgaagg ttttgcaatc gactatgcgg cgcatggggg cggggcggcg ctgggactgg
     5401 tcttcggcgg cctgctgctt tggctctggc cggcggatca cccgcatccc ggcgggcggg
     5461 gtttcgccaa agcggtgacc gtcctgggcc ttgccggcat cgtgttcgcc gtggtccagc
     5521 tcttcggcag cctgagcctt taccgctatg tcggggcgat gatccccgaa caggccattc
     5581 ccaagaccga tgcggaggcc cgagccgccg gcccgcactt gagcgagcga tatccccttg
     5641 atccgcgtag ccacttctat cgcgcccttg accggatcgc ggcaaaggac ctggccgggg
     5701 cggaaacggc cctccaggag gccgttcgcc ttgccgatgc ctggccgcag gtattccccg
     5761 ccgatttccg ggtgcggctg catatggtgc aagcgctggt gctggccgat cgtggcaagt
     5821 tggagaccgc ccgcgccgtc gctcgacccg cctgcactgc gcaggtggac gacagcattc
     5881 gcgggccgct gcgccgggcc ggcctgtgcc gctgatcagt gatcagcacc cttgctgtcg
     5941 tcggccgata ccttggctga gggggacgca tgatgctcgc cgagccgatc ggtccaggcc
     6001 atcagcaaac ccgagacaac gaagacgagg tgaatgatca ccagccacaa gacctcacgc
     6061 tctccgacat tgcggatgtt catgaaggcc ttgagcagct ggatcgccga aatcgccacg
     6121 atcgacgaca tcagtttgag cttgagaccg gaaaaatcga ttttgcccat ccattcgggc
     6181 caatcagcgt gggccccagc gtcgatccgg gagacaaagt tctcatagcc ggagaagatc
     6241 acgatgacga tcagcgaagc ggtgaaagtc agatcaacca gcgccagcgt catcaggatc
     6301 acatcggcct cggtggccgt gatcaggtgg gtgatcccgt gccagacctc ctgcaggaat
     6361 ttgaccagca gcgcacatag tgccagcacc agaccgatat agaacggcgc cagcagccag
     6421 cgactggcaa aaagaaatcg ttcgactgcc cgttccagca tccgctcatc tcctctttct
     6481 gcctgccgcc gcgcccgccg accgggttcg gtcaggcgcc gggcatcgcc agcagtctct
     6541 cgctcacgat atcatccgga ccatggatcg aaattcccgg tcgccggagc ctatccgcga
     6601 cgttgatcta ctatacacag ggcgccggat tgactagacg aggaagcaaa tggagtgtgc
     6661 ggacatggcg aagttggtca cggtcttcgg cgggtcaggt ttcgttgggc ggcatcttgt
     6721 gcgtgccctg gcgcaaaggg gctggcgcgt ccgggttgcg gtgcggcgac cggatctggc
     6781 gggatttctg caaccgctcg gcggcgtcgg acaggtccag gccgttcagg ccaatgtccg
     6841 caatccggcc tcggtggccc atgcggtggc cggcgccgat gcggttgtca atcttgtcgg
     6901 catcctttcg gaaagcggtc gcaacacgtt cggggccgtc caggcagcgg gcgcacgcac
     6961 cgttgccgag gcggtcgcgg cggccggtgt ccgctctttc gtccatatgt cggcactttc
     7021 ggccaacgcc agttcggtgt ccgattatgc tgcctccaag gcctatggcg agcaggcggc
     7081 gctgaaggcc gtgccgaatg ccgtcatcat gcgggcctcg gtgatcttcg gccccgagga
     7141 caagttcttc aaccgcttcg cctcgatcgc ccgcatgtcg ccggttattc cgctgatcgg
     7201 cggcggtgag acgcggttcc agccggtctt tgtcggcgac gtcgccgagg ccattgccgt
     7261 cgcgctcgag ggcaaagcca agcccggaat gatctacgag ctaggcgggc cggaagtggc
     7321 aagcttcaag acgctgatgg aatatgtgct caaggagacc ggtcggacac ggatgcctgt
     7381 caaactgccg ttctgggctg cgcgcatgca ggccagcgtg ctgcagatcc tgcccaaggc
     7441 gccactgacg gtcgatcagg tcaatctgct cgaatatgac agcatcgtca gcccggaagc
     7501 cgcgcgcgag ggacgcaccc ttgccggtct cgggatttca ccgacgacgt atgaatcggt
     7561 ggttccttcc tatctttacc ggttccgcga gcatggccag ttcgaacgct cccgtgcagc
     7621 gtcctgagcc cggctgcctc gcccaaaacc tcgctcagag ctgaggcagg acccggtccg
     7681 gcggggtgtg gccgtccgtg aaggtcttga tattgatgat gaccttctcg cccatctcga
     7741 ttcggctttc gaaggtggcc gatcccatat ggggcaacag cagcacccgc ccctgatccg
     7801 ccagccgaac aaggcgatcc gaaatggctg gctcatgctc atagacgtcg aggccggcgc
     7861 cggcgagatg gcctgattca agcagatcgg tcagctcttc gctatcaacg atctcgccgc
     7921 gcgcggtgtt gaccagaatg gcatcgggct tgagcaactt gaggcgccgc gccgacatca
     7981 ggtgaaaggt cgcgggcgtg tgcgggcaat ggatggtgat gatgtccatg cgggccacca
     8041 tccggtcgag gctctcccaa taggttgcct gcaattcctc ttcggtgcgg gccgagaccg
     8101 gccggcgatt gtgataatgc acctgcaggc cgaaagcgcg ggcgcggcgg gccacggcaa
     8161 ggccgatgcg ccccatgcca acaatcccaa ggcgcttgcc gtgaatgcgc cggccgagca
     8221 tccagttcgg cgaccagccg gtccagtgcc cgccggggat gatccgtgct ccctccgaga
     8281 accggcgcgc cactgccacg atcagcgcca tggtgaaatc agccatgtcc tcggtcagga
     8341 cgccgggtgt gttggtgacg gtgatgccgc gcttgcgggc ggcggcaaca tcaatgttgt
     8401 caacaccatt gccgaaattg gcaatgagct tgagccgtgg accggccctg gccacaagag
     8461 cctcgtcgat acggtcggtg acggttggca aaaggacatc gcaagcgttc atcgcttcga
     8521 ccagctcagc ctcggacatc ggcgtgtccg cctggttgaa ccggacatcg aacaactcgg
     8581 ccatgcgctg ttcgatcacc tctggcaggc gccgggtcac gatcaccacc ggtctttgtt
     8641 tggtcatggt tgtctggcac tcccgaatcc aggcacattc gcggtgcggc cttaccagcc
     8701 gttaacggtg tcgggcgcaa tctcggcaga ctccgcagcc gcctccgtcc ggtctatcag
     8761 gtgcgtcgcc tgttgaaaat gcccctgggg gcgctggctg tcgagattgc agtgtggtga
     8821 cgatgtgggt ggcgatgaaa acctgttcga tccgttcaag gatggtgtgt gcgctcgttc
     8881 ctgttggact tgggcttgcc gccggtccgg gcctggctgc agagcagatc acgaccggca
     8941 cggcaaccgg cttgcccatt cctcgttatg tcagcctcaa gccttcagat acgccgatgc
     9001 gtgaagggcc gagcaaggac catcgcatca aatgggtgtt caagcgtgaa ggcttaccgg
     9061 tcgagatcac ggccgaattc gagacatggc gccgcgtccg cgatgccgaa gggaccgaag
     9121 gctgggtgtt tcattcgcgc ctgtccagcc gtcggactgc gctggtcgca ccgtggtcga
     9181 aagaagcaaa tcttcccctg catgacgagg gcagtgaaac cagcggaatc gtggcgcgtc
     9241 tgcaaccaac cgtgctggcc aatgtcgaaa cctgcaccgg caaatggtgc cggatcaatg
     9301 gcgaaggttt ccgtggctgg atgaagcagg accaattgtg gggcgtctat cccggcgaat
     9361 cagtgaagtg accggatcgt aaaggcgccg atcaaccagc gcgtggacgc agacgcagca
     9421 ccacgtctat tccggcaatt tccattccgg ccggcggttc aggcaactgt cgcatttcga
     9481 tatcggcatt ggcgatgtcg atcagccgcc cttcaccctc gacatagaaa tggaaatggt
     9541 tgccggtgtt ggtgtcgaaa taggcctttg ccccttccac cgccacttcg cggaggaagc
     9601 cggccccggt gaactggtgc agcgtgttgt agacagtggc caaggacacc ggcacgcgcg
     9661 ccttctgagc ctcttcgtgc aacgattcgg ccgtgacatg ccggtcgccc ttgccaaaca
     9721 gcaaccaccc aagcgccaca cgctggcggg tcgggcgcaa gcccgccgtc cgcagcatgc
     9781 ccttaaggtc atgcacgggg cagccgctcc ggctggagcc gctcgacggc ttgatcggcg
     9841 tatcctgcat cgtcattatg aggaatatag cgctgcctgt gcgtctcggc aacacgcacg
     9901 accaaagttc ggtttctctt aagtaatcga caatctgcca gtttcggagc agcacacgtc
     9961 atgttaatga gctttaacaa ttcgtgaggg atcgtatcac catgtcgcag caggcacaga
    10021 caggccgcgc caagtcgagc tacacctacg acgatctgct ggcctgcggg cggtctgagc
    10081 tgttcgggcc tggcaatgcc cagttgccgt tgccacccat gctgatgttt gaccggatca
    10141 ctgacatcag cgagacgggc ggcgcgttcg acaagggcta catccgcgcc gagttcgata
    10201 tccggccgga tcactggttt tttccctgcc atttccacgg caacccgatc atgcccggct
    10261 gtcttggcct tgatgcgatg tggcagctca ccggcttctt cctcggctgg ctgggcgagc
    10321 caggctacgg catggcattg tcgaccggcg aggtgaagtt caaaggtatg gtcacgccaa
    10381 agaccaggct ggtcgaatac ggtatcgact tcaagcgcgt catgcgcggc cgtctggtgc
    10441 tgggcattgc cgacggctgg ctgaaagccg atggcgagac tatctaccat gcgactgacc
    10501 tcaaggttgg tctgtccaaa cagaaggccg gctgaccggt cgctgcggca agagcaaggg
    10561 actttgacat gaggcgtgtc gtcgtaaccg ggatggggat cgtctcatcg atcggcaata
    10621 atgtgcagga ggtgaccgcc tccctgcatg aagccaagtc cggcatcact tatgcgccgg
    10681 acttcgagga gcacgggttc cgatcgcgtg tctatggccg cccgaccctg gatcctgctc
    10741 aggtcgttga tcgccgcgcc atgcgttttc atggcggggg cagcggctgg aaccatgtgg
    10801 cgatggatga ggcgattcgg gatgccggtc tgggcgaggc cgaaatttcc aacgtgcgca
    10861 ccggtattgt catgggttcg ggcggacctt cgacccgcac cgtcatcgag gccgtcgaca
    10921 aggcgcgaga gagcggcaat tccaaacgcg tcggcccgtt cgccgtgccc aaggcgatgt
    10981 cctcgaccgc ctcggcgacg ctggcgacct ggttcaagat caagggcgtc aattattcga
    11041 tttcctcggc ctgcgcgacc tccaatcatt gcatcggcaa tgcctatgaa ctgatccagt
    11101 ggggcaagca ggaccgtgtt ttcgccggcg gttgcgagga actcgactgg acgctctcgg
    11161 ttctgttcga cgccatgggc gcgatgtcat ccacctacaa caattcgaca gcctcccgtg
    11221 cctatgacaa gaatcgtgac ggctttgtca ttgccggcgg cgccggcgtt ctggtgctgg
    11281 aggatatgga ggtcgccaag gcgcgcggcg ccaagatcta tgccgaaatc gttggctatg
    11341 gcgcaacatc ggacgggtat gacatggtcg ctccctcggg cgaaggcgcc gtacgctgca
    11401 tgcagatggc gctcggctcg ctcaaggccc cgatcgacta tatcaacccg cacggcacct
    11461 cgaccccggt cggggacatc aaggaaattg aagcggtgcg ggaggtgttc ggtgcccgag
    11521 cgcaggcgat cccgatctcg gccaccaagt cgctgaccgg ccattcgctg ggcgcaacgg
    11581 gggtgcagga ggctatctat tcgctgatca tgatgaagaa cggctttgtc tgcgaaagcg
    11641 cccatatcga ggagatcgat cccgacatgg ccgacatgaa cattgtccgc aagcgaatcg
    11701 acaacgccag gctgggccat gtcatgtcga attccttcgg cttcggcggg accaatgcaa
    11761 caatcattct caagcatccc gatgcgtgag accaacaagc cgattgcaac gatctgcaat
    11821 catttgtgaa tttacccgag gccgggttgc cgccaaaagg cgtgacaaac caaggttggg
    11881 tgggggacat ccagcatgct gatgcagaac aagcgcggcc tcatcatggg ggtcgccaac
    11941 agcaattcca ttgcctgggg catcgccgag atgctgtcga ggcacggggc ggagctggcc
    12001 ttcacctatc agggcgaagc gctgggcaag cgtgtgcggc cgctggccga gggaatcggc
    12061 gcacgcctgg tgctgccatg cgatgtcgag gacatttcat cggtcgacag cctgttcgaa
    12121 acgctggacc gggaatgggg gtcgctcgat tttatcgtcc acgccatcgg cttttccgac
    12181 aagaatgaac tcaggggccg ctacgccgat accagccgcg acaatttcgt ccgcaccatg
    12241 gtgatctcgt gcttttcgtt cactgagatc gccaaacggg cggcggccct gatgccgaac
    12301 ggcggctcaa tgattacgct gacctacggc gggtcggtgc gggtcatgcc caattacaat
    12361 gtcatggggg tggcaaaggc ggcgctggaa gcatcggtgc ggtatcttgc cgccgatctg
    12421 ggcccgcagg ggattcgcat caatgcactc tcgcccggtc cggtccgcac acttgccgga
    12481 gccggtatcg ccgatgcccg ctcgatgttt tccttccagc agaagcatgc accgctgcgc
    12541 aagtccgtgt cgctgcagga gatcggtggc tcggcgctct atttcctgtc ggatctgtcg
    12601 ggcggcgtca ccggtgaagt tcaccatatc gatgccggct acaacatcat ttcgatgccg
    12661 cgccgcgagg atatggaaaa gcccgagtaa tactggtggt cctcgagttt ggcccgtcgg
    12721 tcattccgga ggtttgctgg catcaatcga ccgctccata cgccagagat cgtgcgcccg
    12781 ctcagaatgg cagcggcacg ccgagtgact ggcgcagcaa gtcaagcgcg atcagcgtca
    12841 acaacgtctt gagcacgatc gaaaagaacc gttcgctcat ccgttcaagc agttgcgttc
    12901 cgagccaggt tcccgcaaag cccgaggcaa tcatggcggc catcagcggc acccattgcc
    12961 cgaaggaaaa tccgatcaac ccgaaggcaa cggccttcag cgtatgctgg atggtcatgc
    13021 acatggcttg cgtagcgacc atctgacgac gctcaaggcc caataccttg agcatcgcat
    13081 tgatgaacgg cccggtcgcg ccgacaaaca tggtgattac cgacgagaca gcgccgccga
    13141 gcacaacacc agagcgctcc agacccggaa tgcccggctt gggaacccag gtcatcgcca
    13201 ggatgaagac accgatcagc gccagcagca cccgtaccgg cagcgcaaca aacagggtgg
    13261 ctcccaacgc aatgccaaaa atggaaccga aggtgaatcg ggccgcgatc ggccagacga
    13321 catggacacg ctgaagcacg gtgcggccga tgttggaacc gatctgcaca accgcatgca
    13381 ccgccacgac catagtcggc tcaacagtgc tggcaagcgc gccaagcatt gcaacgcccc
    13441 cgccgatgcc aaaggcggcc gtcagagccg aggtagcaaa ggagacggca acaagcgcga
    13501 gggcgacaaa cggggatatc ccgggcggca aaaggtcgat cataggctct tgtgccgccc
    13561 gctgcggtca atgtcaaagc ataggtcgca aagaccaggc agctggtgca tgccgcgatt
    13621 aagtggcagc cggctaatcg ccaaagcatg ctctaaacac ttgataacga tcacgttata
    13681 ccagtggccc gcacaaatga cctgccggtc attttccggg ccgtccctcg caaccgggcc
    13741 ttcggccctg ctcggcgtcg cattcgctcc taaccagccg cctgacaatt gcccctaacg
    13801 gtcaattgtc agggccgcgg gtattatctc acgttctgat gattccatca aaacgagagc
    13861 gtgatctagc cgcgtgtctt cagggtctgc aggatgcgat agaccgagcg ctccgaaatg
    13921 ccgagcgcct cggcgatctt ggcgcgcgaa aaatgccgcc gctcaagcag catctcgatg
    13981 tactgcgcct gcaattcatc catcgtcggc aggtgtttga aggtgaagcg gatgccctca
    14041 tcgggcgccg gcggctcggc cttgatgccg aggccgagat cggcggcggt gatggccgac
    14101 tcctcgcccg acagaagcac ggcgcgctcg accacgttgc gcaattcgcg cacgttgcca
    14161 ggccattgat aggtgcggat ggccgccacg gcgctgtcat ccagcacctt gtccacgcca
    14221 cgatggaaat tccgcgtgcg aacgaaatgc cgagccagct ggacggcgtc gtcgccccga
    14281 tcccgcagcg ccggcacatt catcacaaag cgtgacagcc ggtaatagag atcggtgcgg
    14341 aagcggttct gcgcccccat cgccacaaga tcgcggttgg tggcagcgat gaaacggacg
    14401 ttggccttca gatcggtaac gccaccgacc cggcgatagg tgcccagctc gagcacacgc
    14461 agcagtttgg cctgcaggcc gagcgacaat tcgccgattt cgtcgagaaa gaccgtgccg
    14521 ccttcggcca cctcgaccag gccgagcttg cgccggtcag ccccggtgaa tgcgccacgc
    14581 tcatggccga agagttcact ctcgaacagg ttttcgtgca gggtcgaaca gtcgacagcc
    14641 acataggcat ctttggcacg cgcggaggat tcgtgaatgg agcgagccac caattccttg
    14701 ccggcgccgc tttcgccgag aatcagcact gtcgcatcgg tcggaccgaa cagggcaatc
    14761 tgccgacgca cctcatcaaa caccttgctg gtgccaacca tcgtggtggc gaccagcgat
    14821 tgcaggcgat ccttgaggaa atcgccgctg cgcttgagtt gggcatggtc cagcgcgcga
    14881 gttatcgcca gctcgagttc ttcggggcgg atcggcttgg tcaggtaatc atgcgcgccg
    14941 agcttgagtg cccgcacggc ctgatggatc gcgccatgcg ctgtcagaac gatcaccggg
    15001 cagatgtgct ggagttcctg cagaaaatcg agcccatcgc gatcgggcag gcgaatgtcg
    15061 agcaggatca gcgccggctc ttcctcctcg atggcgcgct tggcatcctg ggcgcgcagc
    15121 acacccttga ggttgtagcc cgcacgcgac aattgcgaaa tgatcagccg gttgagggtc
    15181 gcatcatcct cgatgagcag aatcggcttg gcgatgcgtg gcggcaccgg cagttcggta
    15241 cgcctggcgg tatcggcttc ataacgcgtg gccatgctgg gcctcctggg atgcgtccgg
    15301 atccggcagt gtcatggtaa aggtcgagcc aacgccaagc gtgctcgtta cggtgattgt
    15361 tccacccaga tggctgatca ctgatcggca gattgcgagg ccaaggcccg tgccattgac
    15421 cccgtctgcg cgccgactcc agaatggcag gaagatattg ttcaggtgtt cctcggcaat
    15481 gccgacaccg gtatcgctga ccgacagact gaccgtctgc cgggcgccac ggcgcccgac
    15541 cagcgtcagc tgcccgcctt gcggcattga atggaaagcg ttctgaatga ggttgatcag
    15601 cacgatcctg agatcgctgt cggagcccag gacgcgcagg tcgggcgtaa gatcgctgga
    15661 catgacgaca tgaccatcct ctgcctggta gcggagcagg ctgacaatgt cggcgaccac
    15721 ctcgttcagc gagatcagtt gcggctcgga ccccggcggc gtgccgagct tgagcaggct
    15781 gtcggtgatt ttcaggcagc gatcgatctc atgcccgatc agccgcatgg ccatctcggc
    15841 ctcggcagcc tcaccacgcc ggagattggc ttcggtgttg gcaagcagca tggcaaccga
    15901 tgacagcgga ttgcggatct catgtgcgac accggtcgcc agctgaccga tttccgacag
    15961 ccgctgctca tgcgagatcc gcatttcggc atccagatcg cgaatcacct ctatgacaag
    16021 gaaacggttg ccgtcagggc cttccagcct gacccgcgcg gcgttgattt ccacgtggat
    16081 cacccccccg tcaggccggg tgtggtgcga ttgatacttg agaacactgt tcgtatccgt
    16141 cagggcgacc agtggacagg taaccagcgt cggcacacag ggctcggtgc gattgccgga
    16201 cgattggtaa cagaagcgac cgacctcgga accgctggtg ctgccaacat gttcgcgata
    16261 ggtccgattg gcatggacga tccgatagtc ggagtcgatc acccgtatgc cgtcgggcat
    16321 ggcatccagc agagcctgca cgaagcgttc tctggccgcc agttcactga ttgatcgggc
    16381 cagacgctcc gccatgtcgt tgaatgtgcg gcccagatcg cccaattcgg tctggctggc
    16441 accatggacc cggcgcgtga ggtcgcctcc cgccagcgca cgacttgctt cagcaaggct
    16501 cgcgaccggc gccaggacat gccgatgcag ggcatgatag gtgccggcca acagtcccag
    16561 gacgatgagc aggcccgccc ccaccagctg gagggcgccg gtcagggcct ttttctggac
    16621 atcctgggca tgataatcga ccaccagcac gccattgaag ggcgtgagct gcggattgcc
    16681 atgacactgg ctgcatgctt cgcggttggc cacggccttg acgctacgca gtacaggcat
    16741 atcggacagc ggaccgcttt ggagaatttc actccgctcg agcagcgact tgccatccca
    16801 cgcacatccc ggacaaagag cgccagaggc gaaggaaaag cttcggtcga gcaaacgggg
    16861 atcggaggca aaccgcacct cgccctgcgg gttgaggatc atcaccgagg cgatgccgtc
    16921 ctgccgtccg agccggtcga tgatgtcctt gagaccagga atgtcacgct tgagcatcgc
    16981 gttctcaagc gaaacctgca gcaaaagccc aagccgcgtg gccgcttcgg cacgctggtc
    17041 cttgatctgg ttctggtaga cccaggcaaa tccgccgatg aacagcaatg ccacaagcag
    17101 gctgtagaac agcaacgagc gtgtcaggat ggcgaacagg ctcgtcgaac gcatgtcact
    17161 gaaaccgcat tgaggcgagc tggaccagca gcgattcggt cggaaggtct tcctgtgtca
    17221 ggcggacaat gcgctcgtga tcagcaattt cgcgaccaaa aagcgccatt ggcagcacaa
    17281 cgacgttgga acgcggtgca gccaggaagc cttcgacaaa gctgcgccga tcactggctg
    17341 acctggtcgc cagaagggcc tcggcattat gcagaacgat gccgcgctca ttggcttggc
    17401 ccgcgatggt cgcctctatt gccgtgagcg gcacagtatg cgcattcgat ccaagtggcg
    17461 ccatcaccag acggcgaaaa tcgacatgcg cacattggag cgctgccgcc atgtcctgca
    17521 caagatcggg ccgataggaa gcgaaatcga caacaaagcc gcctggcccc agacggccaa
    17581 tggcatcgat caccctctga caccagtagg tcatgcgtct ttgaacacct gatagagttt
    17641 cttgaaatga cccgttcggg cacaaatgcg acacaaaccg cccgcgtccg gtgcggccac
    17701 cccactgagg gcataatagc gcactccgca ggccctgcat cgatatggat caaggccaat
    17761 ctgctggctg gcggcaagtc catccctgtc aagcgaaacg gcatgctggt cacaaacgtc
    17821 gatgcaaagc cggcagccgg tgcacgcaag gggttcgatg acataggccg gccccgtttc
    17881 ggtggcgacg ctcaacatgg caccatccgg gcaagcgcgg atacaggcat cgcaggcaac
    17941 gcagcgcgca ctgtcgatct gcggtcgcag ggcggcccgc ggttctgcgg gttcgggcgg
    18001 aacggtcgcc acaacggcag gaggccgatc aacaaaggcc gaaaacagcc gccgccggct
    18061 cctgtccacc ggatcgacat cctccttcgc ccgcgccagt gcgcgactgt aggcgtctgc
    18121 atcaaggcga cgaagggtcg tgaccggttg ccggcgactt gcgcgcagcc gtgcgtgccg
    18181 cgcagccgct ccctccaggt ccggaccttt gggggcccga tcgcaggtgg cgcaggcgcc
    18241 ggttgcgatc gtcaggctgt tgatcccgtc atcggccatg cggtcgagat cgcgcagacc
    18301 gatggcatgc acacaggcca atgttgcgcc gggaacaccg gaccgctggc aggcggcaaa
    18361 agcatccgac tggtcctttg cccgggacag gatggccatc gggcgcggca tctgcagggc
    18421 ttcgcgcggg caggccggca cacattgtcc gcaagacgtg cagcgatccg ttgccagcgc
    18481 cagcccctcc tcatccagca gccatgcacc cgtcgggcag atttccacgc aagccctgca
    18541 cgaggcgaag ggagccagcg catgcacgca gcgatcggca tcgatttcgg gaaagcaaga
    18601 cccgagcgga ttcatcacca actgggcgct gcgccgggca gatatcgctc ggaggcctgc
    18661 ttctgcgcct cttcgcgccg gcgccgcttt tcttcctcga tcggctcaag ctcaatcagc
    18721 ggcatatctg ccacgtccac gagaacatcg cgaagtcgat gcaataccgc cgcagtcacc
    18781 agcgcaagcc ctgcataaaa ctgcttctcg cagcgctggg caacgcgcat ggagaacggc
    18841 tcgatccagc gcaggatgtg gtgatcgatg aatgcggccg catccttggg cgcgtgctca
    18901 accgggtggg tcagcagatg cgcgatgaat tcaagctggt gaacgagatg atcgtccgga
    18961 cgcttgcgcc agtcaccggc cgcaaccccg taatggtgat accagcgcct gatctcgaac
    19021 atcgcctcct gcatgatcag cccctcggga tcgacccagg tgctttcaag cggtgacagc
    19081 gaatagctgt tgttgagata gatggcggca aaatcggccg caagttcgtc catatgctgg
    19141 gatgtgccgt cgcattcgtc cgaggccagc gcggcgtcga acacggcaaa cgcactctcg
    19201 ccggccgcat cgcctggacc aaggaagagc cgctcggcca gcgggccgcg acgcagttcg
    19261 cccaacaacg gagcggacac ctcacgatca tgcaaagcgg ccaacagaag caaatcctcg
    19321 gccatcaggc cgaggatctc gcggtggtcg ttgcctcccg gctgcccggg atggtcgcgc
    19381 agaccaatgt tcatccaacc ctacccttgg agtgcgggat aaagacgatc gaaggattgg
    19441 taagttcggg atcggccatg ttcttgacct gccgcaccac ttcgtcgttc ttgccccgcg
    19501 ccttggtctc gtagctgcca tcgcgcaact tgtcgatggg gccgatatcc agcacgcgca
    19561 tcatgcaccc catgacgcaa tacggcttgc ggccggcgtc gatctcgtcg acgcacatat
    19621 tgcacttcat gaccgtattg gtctcggggt tgtactgcgg agcgccgaac gggcaggcag
    19681 cctcgcaacg gcggcagccg atgcacagcg tcgagtcgat gtcgacgatg ccgtccttct
    19741 gccgcttgaa gatcgcaccg gtcgggcagg tgggcaggca cgccggctct gcacaatggt
    19801 tgcacgacat gttgatttta taggcgtaga cctcaggata ggttccgccc tcgatatact
    19861 gcacgcggcg aaagcgcggc cccggtggta ggccgttctt gtccttgcag gcgatctcgc
    19921 aggcgcggca gccgatgcaa tccacattgt ggtggatgaa gccgagctgc atgtccggct
    19981 tgaccggaac atagacctcg cgcttcttgc gcttcaccgc ttccttgatg gcagccttgt
    20041 tggtttcgtt agccatgatc agcccccatc acatatcgcg gcggaacacg tggctgcgcg
    20101 ctgtcgcaag ctcgtcccat ccaggcttat ggacaagatc ggtcttcttg atcgcgcaga
    20161 gcacagtatt gtaggcaaaa gcacccgccg gcgagggatt gtcgtcggtg aggaagtcgg
    20221 gattgccgga gcgatccacg ccgtccttgt cgacatccat ccacgcccct tcgtgcagca
    20281 cgagaacgcc cggcatgcag cgttcggtca cgtaggccgg cacaaccgtg cgaccgcggt
    20341 cattgtagag ttcaaccgta tcgccggtct tgatgccgag cttggtggca tcggaggcat
    20401 tgatggtgac ctcctgcgag aaggtttccc gcagccaggg gatattgttg aagatcgagt
    20461 gcgtccgcca gcgcggatgt ggcgagacca gatggaaggg gaacttcttg gtcagcggat
    20521 ggttgagcga ttcccatggc tcgatccact tcgggaggta tggaatctcc tcgccatact
    20581 gagtccgctt ccagtcggtg atctgggcga gctgggtcga aaagatctcg atcttgcccg
    20641 atggcgtcgg gaacgggacc cccttctcga tctgctgctt gaaggcgaca tgcggctcgg
    20701 tcagcaggaa cttgtagatg ccacgctgct tgaactcatc ccaagacatg gtcaccttgg
    20761 aatacttgtt ctgcatcact ttgccttccc accactcacg caggtaggcc tcgtcaacgt
    20821 tgtcgtcgtt gaggaagtaa tcgcggttgg ccttggggtt gtaggcaggc ccgaagccaa
    20881 gccggtaggc cagttcggtg aagacctgga agtcggtctt ggattcgccg agcggctgaa
    20941 tgaccttcgg acggtggatg tagtaatggc ccttgtacca gggcagcgcc acgtcatgcc
    21001 gctcgaaatg cgtggctatc gggaagagca cgtcagcgta aaggcccgag ggggtgatgg
    21061 tggaatccat gcacaccacc agttcgagct tcttgatcgc ctggatctcc ttgttgatgt
    21121 tggtgagctg gttgaaccag tccgacccct gccagaagat gcccttgatg ttgggaatga
    21181 ccccgtcaag ctgatccccg cgcggccaga gcccaacctc ctcacgcttc acattcggat
    21241 agttgagcac gatatgggcc cagcgatccg acttgatcgc cgcgaaccat acgttggcaa
    21301 actcgtcata gggataagca acagcgtcgt tcttgaagcc cttgccaacg ccctccgagc
    21361 agccgcccag aacgccgatg ttgccggtca tcgcctgaac ggccgaggcc atacggttgt
    21421 attgctcacc ataggcattg cggcccggcg accacgaggc cttgagcgcg gccggcttgg
    21481 tcgtcgcata catgcgggcg agcttgatga tatcgtccgc cttcacgccg gtgatcgcct
    21541 cggcccattt cggggttttc ggcgtcttgt catacttgcc gaggatgtaa tccttgaagt
    21601 tttccttgtc cttggcccag tccggcatgg tgccggcatc cattccgagg acaaacttgt
    21661 tgatgaaggc ctggttgtgc aggttttcgg tgaagatgac atgggccatc gccgcgagca
    21721 tcgccgcgtc ggtattcggc cggatcggga tccaccaggc atcataggcg gcagccgaat
    21781 ccgtatattg cgggtcgacc agaacgaact tgcagccgcg ctgtttggcc aggcgcatat
    21841 agtagaaggt gttgccaccg tggaatgtat aggccgggtt ccagccccac atgatgatca
    21901 gcttggaatg ggcgaaggca tcgtcctcgt cgccatcctc gatcgtgccg aaggtcatgc
    21961 gcgaggcaaa cgtcgtgccc tgataggagg ggactgacca cgacgaggtg cggcagccga
    22021 acatgccgag gaagcggccg agcagacctt cgatctgatc agacttgtgc aacacgccat
    22081 aggaggcgcc ggcgtaggac tggtcgacaa tggcggtggg gccgtatttg gccttcagct
    22141 ccatcatctt gttggcgaca taggtgagcg cctcgtccca cgacacgcgc ttgaacttgc
    22201 cctcgccgcg ctcgccgaca cgcatcatcg gatagagcag ccgctccgcc gaataaagac
    22261 gcgcacggta ggagcggccg cgcagacagg cgcgcacctg cggctcttcc tcggtgtcct
    22321 tgccgaagaa accgtccttc tgatagcgcc catcgtcagt cgacagacgg acgatcacat
    22381 cgcccttctt atgggcgatg agtgcgtggc gcgagccgca gttatgggcg cagcttgtca
    22441 caacggtttc aagctgatcg tcggtggggt agggctcata ggcgaaggca ttggccgggg
    22501 ttggcgccgc agtcggcaac aatccggcag cgccacccac tgccgcaagt tcaagtaagc
    22561 gccgacggtc aagcttcagc gcgccgttga gccgcttagg atcgagacga tcgttcacga
    22621 catcctcctt catttgccgc tgataagata acgggattcc gacttggtcg ggcgattctt
    22681 cgcccaatcc ggcacagtct tgtccatgtc cggccacggc gtgcgcggat agggatagct
    22741 gatccgatac caggaacgac cgccatggca gccgtagcag acatccgaag agttctcgga
    22801 agccttgtcg atcccgtcct tgttcagata ggtgacattg tgcttttcgg tccgaattcc
    22861 ggcatggcag gtcatgcagc cgttcggggt gccctcgggc tccagatccg cccgctgctc
    22921 atgccagggg cctgccagcc ccatgttctc gtaggggaaa ttgccatggc acctgaggca
    22981 ggtctcggac ggattgacac gctttctgtt ggtgaagggc gcctgcccat tgttcgacgt
    23041 catcagcgca ttcggaaccg taaaaaccgg tgattgctcg cgcggatccg aaccctgatg
    23101 gcagaagttg cactccagct tcatcacctg cttggccaac gccgattcca gatgccgcca
    23161 atggaacgat tcctgattgc cctcgtagct gtcgagggtc tgataccagg caagcacaga
    23221 atttgccttg gcgcccgcct gagattcagc gcgcggcttg gtcgtcagca cctcctgatg
    23281 acaggtcaga caatcgctgc gcgtggatga ggcaatattc ggaggaaagt ggatcgggtg
    23341 atagcgggcc gtctcatagt cgcccgccag agctttcttc gtctcggcat cgaatttctt
    23401 catcgtgtcg gaacggtcgg caggaagagt tgccgcaggc tttgcctggg cgatcgcttc
    23461 gctggcgatg ccagggcgcc atccgtcggg cgccagaaac tcggctgcgc caatcgacgc
    23521 ggaaagcgcc agcgcggttg ccatccaata ggtcctgttc atggcctcgg cagttccctg
    23581 agacagagtt gaattgagct cggagcgggg ccgaatggcc ccgctccagc tatacggctt
    23641 acttcttctg gccggccatg ccgcccatgc cggacatacc cgacatgccg cccatgccgg
    23701 acatacccga catgccgccc atgccggaca tgccagacat gccgcccatg ccggacatac
    23761 ccgacatgcc acccatgccg gacataccgc tcatgccgcg catagcgccg gacgaccatt
    23821 gagtgcagac ccaaccagga gccgcaccgg ccataccgga cataccggac atgccgccca
    23881 taccggacat gccgcccata ccggacatgc cacccatgcc acccatgcca cccatgccac
    23941 ccatgccgga catgccggac atgccgccca tgccggacat gccacccatg ccagacatgc
    24001 cgcccatgcc ggacatacca gacatgccgc ccatgccgga cataccagac atgccgccca
    24061 tgccgcccat gccggacatg ccacccatgc cacccatgcc ggacatgccg cccatgccgc
    24121 ccatgccgcc catgccggac ataccagaca tgccgcccat gccgctcatt ccggacatcg
    24181 acggacgata gctccactgg gtgcagaccc agctattgcc gccgcccata ccggacatgc
    24241 cgcccatgcc gcccataccg gacatgccgc ccatgccgcc catgccggac atacccgaca
    24301 tgccgcccat gccgcccatg ccggacatgc cgcccatgcc ggacatacca gacatgccgc
    24361 ccatgccgga catgccggac atgccgccca tgccggacat accagacatg ccgcccatac
    24421 cggacatgcc ggacatgccg gacatgccgc ccatgccgcc catgccggac atacccgaca
    24481 tgccgcccat gccgc
//
LOCUS       DAOMXG010000014        24153 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_1297_length_24153_cov_10.069881, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000014 DAOMXG010000000
VERSION     DAOMXG010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 24153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24153
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1297_length_24153_cov_10.069881"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1238
                     /locus_tag="PLQ11_01995"
     CDS             <1..1238
                     /locus_tag="PLQ11_01995"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRK23703.1"
                     /translation="AERLLGREPMTLLLKAQAAQLAGDRPAAEQAFSRMLETDETRLL
                     GLRGLFVEARRTGNDAARSFAEQAYRLAPATPWAAEALLDYRSGEKDWSGARAIVDDS
                     ASRRLVDREVSKRQKAALLTAEAIDLKEQSPDQAFQRATEALKLNPALVPAAALVAQR
                     LIARGETTKAARVLESAWKQEPHPDLAEAYLGVRYGDSAHDKLKRARHLAKLTPGARE
                     ARLTLAAALLDAREFDEARKELEALVLDQPSVRVCHLMAELEDKESGHIGMVRKWLAR
                     AAQAPRDPAWVADGFVSESWQPVSPVTGRLDAFRWTTPPQSIESKLRASIDADRLIAS
                     ARSRDDHAKPTAPAALQAPGPVPAAPQPAAAEPVILPDAVAAEAEALALEPPEQRPPD
                     DPGPRKPEPKARKGWRLFS"
     gene            1304..1379
                     /locus_tag="PLQ11_02000"
     tRNA            1304..1379
                     /locus_tag="PLQ11_02000"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1337..1339,aa:Thr,seq:cgt)
     gene            complement(1495..2451)
                     /locus_tag="PLQ11_02005"
     CDS             complement(1495..2451)
                     /locus_tag="PLQ11_02005"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRK23704.1"
                     /translation="MTTALSPVLMIQSKRERAPGVPLPTGSNRGRTSGNLYNKKPAAG
                     PHVHLFSLMECGILNETSHVLTEKRTGCFMADSAEQSHSWLHQRFLNARNRLQRRSIL
                     NEQDIEARFTKTYHLNYWGSVESRSGSGSTAEATQQIRRMLPEVIKQFGVRRLFDAPC
                     GDFNWMQLLRPSLDVDYTGGDIVRPMIEHLSQTHADARTRFVHFDITRSDFPDVDLWL
                     CRDCLFHLSYADARRALEGFARSSIPYVLMTTHLNDGQFVNRDISTGDYRSMDLFSAP
                     YSLPRDVLFRFDDPTSARREMCLWSREQIVAALAAHSWPAAA"
     gene            complement(2519..3049)
                     /locus_tag="PLQ11_02010"
     CDS             complement(2519..3049)
                     /locus_tag="PLQ11_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4'-dihydroxyacetophenone dioxygenase family
                     protein"
                     /protein_id="HRK23705.1"
                     /translation="MTSPVPPSSDKWLPYRHPMPKESPPELVVPNVIPQDERVWVPTD
                     DNVWFRPLCMVASRGYWVNLLRVRRAGVVSRHRHPQPVHGYVLKGEWRYLEHDWVARE
                     GGYVYEAPGETHTLVVDPHAGEMITLFQVNGAMIYCDPDGTCTGFDDVFTRIDRCRRH
                     YIANGLGEGFIDQFIR"
     gene            complement(3066..3770)
                     /locus_tag="PLQ11_02015"
     CDS             complement(3066..3770)
                     /locus_tag="PLQ11_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRK23706.1"
                     /translation="MKQALVTGGTRGIGEGIARALAEAGFQVLACGLTEAECAAMTPR
                     PGIRTTVLDVTSDASVADALRGMDRLDVLVNCAGIIQRGGVEFDLAAFEKTIAVNLTG
                     TMRMCVTAKELLKTSGGSIVNMASMLTFQGSPFVPGYSASKGGVGQLTKSLAAAWAAE
                     GIRVNAIAPGWIDTPLTRPLVEDAARSAPILARTPMNRWGTPADIGGVVVFLASDAAR
                     FVTGAIVPVDGGYLAV"
     gene            complement(3772..4707)
                     /locus_tag="PLQ11_02020"
     CDS             complement(3772..4707)
                     /locus_tag="PLQ11_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HRK23707.1"
                     /translation="MQEYPDILAIGEPMVEFNQTGGSGTRTYLQGFGGDTSNFAIAAA
                     RQGARVGYVSRLGDDPYGRMLRDLWEAERVDHRFVTTDPAAFTAIYFVTHDASGHQFS
                     FFRSGSAASRMHPDDLPVDAIRRAKVMHFSGISLAISSSACDACYRAVEIARGAGVKV
                     SFDTNLRLRLWSKERARAIITDVIGLSDICLPSYDDVVAITGLSDADAIVDFCLERGA
                     RQVALKRGSEGVLVAEKGRRLGIAPVPCKPVDATGAGDTFGGAFVARLVAGDDLFDAA
                     RYANAAAALSTEGFGAVEPIPDAAKVRAALARTDG"
     gene            complement(4726..5376)
                     /locus_tag="PLQ11_02025"
     CDS             complement(4726..5376)
                     /locus_tag="PLQ11_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HRK23708.1"
                     /translation="MTTDELIARFRRVATASVADAVDKICGRRGYLDSAVKPRINEKK
                     ICGPAATILEGPTTEFVPPQHALDLIDEAEAGSVICIATGGEAEMAVWGGLMTAGAIA
                     NRHEAAVLDGGVRDIVEIRRDYDFPVFARAVSPGTTLGRYKTLAANIEIFIGGVAISP
                     GDIVVGDIDGVVVVPRARAEAVLAMAEEIDVRELEQAKLIIAEKSLRKGLAKYGRI"
     gene            complement(5392..6360)
                     /locus_tag="PLQ11_02030"
     CDS             complement(5392..6360)
                     /locus_tag="PLQ11_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HRK23709.1"
                     /translation="MVHELHPLRVVVVDDGYDSYDTERAVLAAAGATLELRPCRGQAE
                     RVVMAIADADAILVRESPVSAAAIAAAPRLRAIVRYGIGVDNVDRNAASERRIYVANV
                     PDYGTEDVSDHALALLMAVARRIVTRDRAVRAGGWFTGSSEKMYRPFGRTLGLVGYGR
                     IARAFERRMRALGVGRVLVHDPFADLPASAEAVTMERLCAESDYVSLHAPLTPATRHI
                     LNAARIALLKPTAIIVNTSRGPLIDEVALVEALSAGRIFGAGLDVFECEPPTRNNPLL
                     TMTNVVLADHAGWYSEESVADLQKKAAEEVARILSGKPPRHWINPW"
     gene            complement(6528..7550)
                     /locus_tag="PLQ11_02035"
     CDS             complement(6528..7550)
                     /locus_tag="PLQ11_02035"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRK23710.1"
                     /translation="MTIHRRMLLAGSVAIAALFSLPALAQQVTLKYAHFQPARDDQPK
                     HKAALAFKEHVEKATKGSVSVQIFPAGQFGNDAATMEGLKLGTLEMAVAHDGAISTVY
                     RPVGILGIPFMFESHAHAWAVYDSAWRDEFGAGMTKATGIRLLEFADNGIRHFTNSKA
                     AISSPADMKGMKMRIQPSPVFRALVESLGASASAIPWAELPTALQSKVVDGQENGVSN
                     ILAASLFQYQKFVSLTGHVYSSHAYLVNDAFFSKLTADQQKAVREGAVLARDIHRKMT
                     SDQDKDAKRILTEKGLTVTELSAAQIAEFRKLAQPPVVEWANKEIGKEHVDALLKAVD
                     ANRPKS"
     gene            complement(7575..8864)
                     /locus_tag="PLQ11_02040"
     CDS             complement(7575..8864)
                     /locus_tag="PLQ11_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRK23711.1"
                     /translation="MGATLGLVFVLGMLIGLPIAILMGVSGVAALIVDGRFALISAPQ
                     RFFNGINSFPLLAVPFFILAADLMSASAITSRILAFTNSLIGHRRGGLGHVNVLTNMI
                     FAGISGSALADAAGPGAVMMRMMQRSGYEPHYAAALSGATATIGPIIPPSIIMVIYAL
                     ADSRVTVAGLFAAGIVPGIMLGIALAITNHIISVRRGYGGAHERKGWSERLRALGGAL
                     WALLMPVIIIGGIVSGIFTSTESAAVAVAYALFVGLFVTRKLTAELIVRAVLQTALVS
                     SAALIIVSTASLFAWLLTILGVPQAVASWIATLTNDPLIVMFLVAIFVLICGFFIDTL
                     PAVIILVPVLAPLTDIFGINPLHFAMTIVLNLTIGLITPPVGGVLFIITSVSKLRFER
                     IARAIMPMVFAEIAVLVLLILFPALSLTVPAWLGYSR"
     gene            complement(8868..9437)
                     /locus_tag="PLQ11_02045"
     CDS             complement(8868..9437)
                     /locus_tag="PLQ11_02045"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRK23712.1"
                     /translation="MTDCKPDLLSSAMSFPPCPPSGLRRLEWQVVWLNAALVLAITAL
                     MVVLVVGNVFFRYVLNASLIWAEELSQYLMVWMVFLGAGLAFRQGRHVAVEMLQDALP
                     GPTGRMLRWVVLVISAVFLALLVVLGTRYALFAAEQQTPALQISAAVPYSAVPFGAAL
                     FLFHLVMAAPAFVEKRYEEMGSLEQGEES"
     gene            9552..10247
                     /locus_tag="PLQ11_02050"
     CDS             9552..10247
                     /locus_tag="PLQ11_02050"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRK23713.1"
                     /translation="MLTVVPGPVERPKTLTDIAAAAIREAIITGRIAQGEQVSEAQLA
                     GSLGLSKTPVREALLRLQTEGLVAVSPHRGTFVFSLSDGELTHICELRLALETGAVIL
                     ALERNRERLVSGWLAILDRMGAALNLPDPAAYLVSDSEFHEHLIHCSGNRYFQDAYSL
                     IAAKVTTLRLKLGRDSFHMEKSFLEHRLLVDHVRTGAVEQAQQVLRRHIARKEGSYWE
                     QLRPVKSSYLDAS"
     gene            10398..11312
                     /locus_tag="PLQ11_02055"
     CDS             10398..11312
                     /locus_tag="PLQ11_02055"
                     /inference="COORDINATES: protein motif:HMM:NF016894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HRK23714.1"
                     /translation="MSKPALIGVDWGTSSLRLWRFRADGSVSDTKRSDQGIMNVGEGR
                     FRPVFEAAAGAWLEPGVPILMCGMVGSRQGWAEASYAFCPAEVATLADHLKPVPGYPN
                     AFIVPGVAVRAADGRRDVMRGEETQILGAASGPGRHLIVLPGTHSKWVIVENGCIRTF
                     HTFMTGELYSLLRHHSILARLMPQSDAEVDEAAFETAVRGALDGAAAVMQSLFAIRAR
                     GLFAELAPEQAASSLSGLLIGAELREGLALARPLGVDHVAPWVIGSSALAQRYAQAFA
                     VAGFRVRTEDEGASMRGLWAIARQRGLV"
     gene            11346..11987
                     /locus_tag="PLQ11_02060"
     CDS             11346..11987
                     /locus_tag="PLQ11_02060"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010683568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HRK23715.1"
                     /translation="MLDFDGAMASLPLIAVLRGLPPDDAVGVGEVLVETGFRLIEVPL
                     NSPEPLVSIGRLVKEFGSMAVIGAGTVTSQTDAKAVLGVGGRLIVMPHSDPAVIQSAK
                     AGGGIVAAGVATPTEAFAALAAGADALKLFPAEMISPAAVAAMRAVLARSVRLLPVGG
                     ITPALMPAYHAVGVNGFGLGSALYKPGLAREAVAARAQQFVSAWQALTSRSSG"
     gene            complement(12200..12865)
                     /locus_tag="PLQ11_02065"
     CDS             complement(12200..12865)
                     /locus_tag="PLQ11_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23716.1"
                     /translation="MVDATRFQTLKRIAIFAGLGFGIAACATPANQQARVQAYANANA
                     ATAPGQPDDSLETSSINQAKLTHYVEFRSRYAHSYGHTFLVHGRLNGKRIATGEVAGL
                     HPRGDDPSVWVLGHFIPVASETGASDGDTEDQYISARYRIYLTAAQYDKVSAKIRQLQ
                     ASSPMWHAVWYNCNAFVADVAGFMGLQSPNSLELPADFINGMRRLNNSANTIIPAALT
                     IDR"
     gene            complement(13032..13151)
                     /locus_tag="PLQ11_02070"
     CDS             complement(13032..13151)
                     /locus_tag="PLQ11_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007815309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HRK23717.1"
                     /translation="MKKLSMVAVAILVAMSVSACGTVGKGKGKAPAAPIVTKG"
     gene            complement(13780..14115)
                     /gene="grxD"
                     /locus_tag="PLQ11_02075"
     CDS             complement(13780..14115)
                     /gene="grxD"
                     /locus_tag="PLQ11_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019907034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HRK23718.1"
                     /translation="MSDVNARIDAEVTNNDVVLFMKGTPQFPMCGFSGQVVQILDYLG
                     VPYKGINVLEDGAIREGIKAYSNWPTIPQLYIKGEFVGGCDITREMFQAGELQAMLAD
                     KGIKGAARS"
     gene            14272..15240
                     /locus_tag="PLQ11_02080"
     CDS             14272..15240
                     /locus_tag="PLQ11_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23719.1"
                     /translation="MSIGRGIAGIAGLIVAGCATHPLPDWPTGYSTAQIVQHIRCEAR
                     QAVAEVMLEKAGIPVTQSGVDTFTFATREDGSNDPLGKLKAAYRDAAIAFDFKLTMTE
                     LNKFSAGASIADKFNRGHRSGGASSGTLVMGAKLDQTRQNERMFVVSTNLDDLFEGPE
                     APNCRVPASPANPFYPVTGSIGIKEVILTFVELAEASVLRGKDGAGNSRRLVDTLQFT
                     TDTSAGVDSGKLTIAPVGLVLNLSEVSVAGSAGRKDVHQVGIALSLAVVQAAQVVPGP
                     KAHKRARQTEPNPKKQEARQNAVDGFVERDYRRLQDALTVIQRRLE"
     gene            complement(15254..15484)
                     /locus_tag="PLQ11_02085"
     CDS             complement(15254..15484)
                     /locus_tag="PLQ11_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006924640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HRK23720.1"
                     /translation="MAMKAADIEALIREAFPDATVEIRDLAGDGEHYAATVIAPAFKG
                     KSRVAQHQMVYAALKGRMGGELHALALTTRSE"
     gene            complement(15496..17724)
                     /gene="purL"
                     /locus_tag="PLQ11_02090"
     CDS             complement(15496..17724)
                     /gene="purL"
                     /locus_tag="PLQ11_02090"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HRK23721.1"
                     /translation="MPNDIKITPELVAQHGLKADEYQRFLKLLGREPTITELGIVSAM
                     WNEHCSYKSSKVHLKTLPTKGERVVHGPGENAGVVDIDDGDVVVFKMESHNHPSYIEP
                     YQGATTGVGGILRDVFTMGARPIACLNALRFGDPSHPKTRHLVGGVVAGIGGYGNSFG
                     VPTVGGQVGFHPRYNGNCLVNAMAVGIAKRDLIFTSMATGVGLPIVYLGSKTGRDGIH
                     GATMASAEFDENSEEKRPTVQVGDPFAEKLLLEACLEIMAKGCVVAIQDMGAAGLTCS
                     AVEMGAKGDLGVELNLDNVPCREERMTAYEMMLSESQERMLMVLHPEKEKEAEDVFRK
                     WGLDFAIVGHTTDTLRFVIKHEGEVKADMPIKELGDQAPEYNRPWVAPAPRPGNSALI
                     NGIVSVAEAKAADHAAALLKLLGSPDFCSRRWVWEQYDHIILGNTVQRPGGDAAIVRV
                     GEGPKGLALTCDVTPRYVEADPFEGGKQAVAECWRNLTATGSTPIALTDNLNFGNPEK
                     PEIMGQFALAIKGIGEAARALDFPVVSGNVSLYNETQGKAILPTPTIGGVGLIKDVTQ
                     SVGIGFARPGEAIVLIGRTDGWLAASAYLATILGREDGAPPPVDLAIEKRNGDFVRSL
                     ILAGRVSACHDLSDGGLAIALAEMAMAGGLGASIERLPAGIAPVAALFGEDQARYVIS
                     VPAAKAEALIAEAGKAGIAALLLGTTGGATLTLPGCAPLSVAQLSKAHEHWLPAFMSG
                     QA"
     gene            complement(17796..18443)
                     /locus_tag="PLQ11_02095"
     CDS             complement(17796..18443)
                     /locus_tag="PLQ11_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HRK23722.1"
                     /translation="MRIIRTDADIEEGMAHLGKADPRLARVAAIAGPIPLRRHVPGLA
                     GLCSIIVSQQLSVASADAIWRKTKAGLGEITAEAILGADEATLRAFGLSTPKMRTLRG
                     LAEAVRSGTLPVDALCDMPGEEAMTALTSVPGIGPWTAEIYLMFYAGHADVFAAGDLA
                     LQEAARLALGFDARPSARELAVLAEGWRPWRAVAARLLWAFYRVAKARDGITGGG"
     gene            18473..19321
                     /gene="gluQRS"
                     /locus_tag="PLQ11_02100"
     CDS             18473..19321
                     /gene="gluQRS"
                     /locus_tag="PLQ11_02100"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HRK23723.1"
                     /translation="MQPVFRFAPSPNGFLHLGHAYSALLNQHLARESGGLLLLRIEDI
                     DTTRCRPELLEAMFEDLEWLGLEFDQPVLRQSTEFARYRAVLERLEADGLVYPCFCSR
                     KDIAAAAGDHPVRDPDGAPLYAGTCRRLSNAQIRQKRSEGGAFALRLDMAAALARIGT
                     RPLDWQEAGQGRIAAEPALWGDVVLGRKEVPTSYHLSVVLDDAMQGVTDIVRGRDLFS
                     ATSIHRLLQVLLDLPEPRYRHHRLLTDSDGLKLAKSRSSPALRQLRSEGVSAADLRLH
                     LGFAPI"
     gene            complement(19314..20234)
                     /locus_tag="PLQ11_02105"
     CDS             complement(19314..20234)
                     /locus_tag="PLQ11_02105"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HRK23724.1"
                     /translation="MIETFIILFPIFAVILLGWLAVRLRFVGVESGNGLSEFVFVIAI
                     PLMLFRTIATAPLPASPPWSYWLSYYLAMAIVWTIVAQVARRAMKRDNREASIIGFSV
                     GQSNLVLISIPMVLRVFGEEGSVPLFLMVAVNLPITLTVATVLIETSDAPAGRYRAML
                     VKLATNPILIGIAAGLLWRQTGWLIPDPVMSSLKFIGDASAPCALFAMGAALSRYGLG
                     GDYRLLALVVALKLLVFPAIVYLLAYKLFALPPVWAGVITMIAAAPCGINAYLLAERY
                     RLGLGLTSGAIAVSTVLSVVTSTFWVWLVR"
     gene            20393..21511
                     /locus_tag="PLQ11_02110"
     CDS             20393..21511
                     /locus_tag="PLQ11_02110"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HRK23725.1"
                     /translation="MQGQQIERLRDLAHDLRSPLGGIDSMIELLAKTRIGPDQDVLIQ
                     GLQAASAHLRAVAGAMLAPAEVPGQEATTPLMQVLRTFSPSARARSRVREQYYAEAID
                     PSCRTILIGDATALRQVLENLVDNAFRVAPGGPVSLAVGEAIRPDGMAVVRLALSDKG
                     PGLTEAEAAVVFERGTTLADRQAGTGLGLAIVRRLVEQAGGSCGASSPGKGQGATFWV
                     EWPVAGRQQEFAESSPMPAQTDGGPQIVIVDDDATSRNLLKLLLDHFELPAVALASPL
                     EALRVIESQRPAAILTDMSMPQMDGLAFVEAVRGLFDVESCPPIIAVTGRVHPEDQKA
                     MREAGCAAIVEKPLTIHDLRQALKATGLTGSGSTVKAA"
     gene            complement(21512..23488)
                     /locus_tag="PLQ11_02115"
     CDS             complement(21512..23488)
                     /locus_tag="PLQ11_02115"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRK23726.1"
                     /translation="MSGTQVYIPDSTIDLGVEPPLALFNRSGEPPPKGRISARWLAGT
                     VLTGFAGMGLMLAAAISAIEHRGAIVELPHMIKPREIASQLPGELVIAARKGDRLVQK
                     ADIVAARQTYKTPVVRKTAGGDVIRPASFTRISSPLAMETLGFANVIPAFNTARLIAD
                     ASEDRALEAAQGMPLDSDVSLSTRTIEDGDRPALFLTSLSEVEVQAQVLEALSATRRT
                     APTLPAQMLLAKAMRAPEQTSSLPFAGTNDPFSRLVVRMVPENVTVAPRLEAALARNA
                     QEQRLVGIRSEDNAEQVLRAAGLTPSQARDIVRSLTRGGQVLDTSKRLKLTLQALEPG
                     AAKSVVRVDLYNEEIRAAAAGRKDDGSFVLIEATRPAAPSRRTTGEDDEGEGGFSLFS
                     SLHETARKHGIPPPMIDEIVRVLFFDVDLQRAVGSNDSIEVLIGNDDSDTPGELMMLS
                     LTFGGETRKFYRFALPDDDVVDYFDDQGRSAKKFLIRKPITDGELRSTFGSRRHPILG
                     YWRQHNGVDWAAKIGTPILAAGNGTVRFAAWDSGYGRRVEIQHTNGYVTTYSHMSAFG
                     RGIAEGVRVRQGQVIGYLGSSGLSTGPHLHYEVMIDERYVDPLAIRLPRGRELNGPQL
                     ADFRRQREQTDQVLKKAPGSAASLTAAGQASARP"
BASE COUNT         4669 a         7296 c         7730 g         4458 t
ORIGIN      
        1 atgccgagcg gctgctggga cgcgagccga tgaccctgct gctcaaagct caggcggcgc
       61 agctggccgg tgaccggcca gcggcggaac aggcattcag ccgcatgctt gaaacggatg
      121 agacgcgcct gctgggcctg cgcgggctgt tcgtcgaggc acggcgcacc gggaacgatg
      181 cggcgcggag cttcgccgag caagcgtaca ggctggcgcc ggccactcct tgggcagcgg
      241 aggcgctgct cgattaccgt tccggcgaga aggactggag cggggcgcgg gccatcgtcg
      301 atgattccgc cagccggcgg ctggtcgatc gcgaggtatc gaaacgccag aaggcggcgc
      361 tgctgaccgc cgaagcgatt gaccttaagg agcaatcgcc ggatcaggcc ttccagcggg
      421 caaccgaggc cctgaagctc aaccctgcgc tggtcccggc ggccgcgctg gtggcgcagc
      481 ggctgatcgc gcgcggggag acgaccaagg cggcgcgcgt gttggaatcg gcctggaaac
      541 aggagccgca tcccgatctc gccgaggcct atctcggcgt ccggtatggg gattcagcgc
      601 atgacaagct caagcgcgcg cggcatttgg ccaagctgac gcccggcgcg cgcgaggcac
      661 gcctgacatt ggccgctgcg ctgctcgatg cacgggaatt cgacgaggcg cggaaggagc
      721 ttgaggcact ggttctggat cagccgagcg tgcgtgtgtg ccacctgatg gccgaactcg
      781 aggacaagga gagcgggcat atcggcatgg tccgcaagtg gctggcgcgg gcggcgcagg
      841 cgccgcgtga tcctgcctgg gtggcggatg gctttgtatc ggaaagttgg cagccggtca
      901 gtccggtcac cggccgcctc gatgcgttcc gctggacgac gccgccccaa tccatcgaat
      961 ccaagctgcg cgccagtatc gacgccgatc ggcttatcgc gtcggctcgc tctcgggatg
     1021 atcatgccaa gccaaccgct cctgcggcgc tccaggcgcc cgggccggtt cctgccgcac
     1081 ctcagcctgc cgcagcggag ccggtgattc tgcctgatgc cgttgccgcc gaggccgaag
     1141 ctctggcgct tgaaccaccc gagcagcgcc cgccggacga tccggggccg cgcaagccgg
     1201 agcccaaggc gcgcaagggc tggcggctgt tcagctgaac cggtgcgcag caggaacagg
     1261 cctgttgcac tcggagccga ctagggctat ggcagcaacc gatgccgcaa tagctcagct
     1321 ggtagagcac gtcattcgta atgatggggt cgggggttcg attccctctt gcggcaccac
     1381 tttccccttc aagatctatc aaaagctcca ccgaccgttg cgttttcgac gcgtttttct
     1441 ttgcgcgaac cggactccgc ttcgcttgaa attgctctgg acggtgtggc cttttcaggc
     1501 ggcggcgggc caggaatgcg cagccagagc cgcaacgatc tgctcacggc tccacagaca
     1561 catctcgcgg cgggccgagg tcggatcatc gaagcggaac agcacgtcgc ggggcagaga
     1621 atagggcgca gaaaacaggt ccatgctgcg gtagtcaccg gtagaaatgt cgcggttgac
     1681 aaactgcccg tcattcaggt gcgtcgtcat cagcacatag ggaatcgagg agcgcgcaaa
     1741 gccctccaat gcgcggcgcg cgtcggcata agagagatga aacaggcaat cgcggcacag
     1801 ccagagatcg acatcaggaa aatcgctgcg tgtaatgtcg aaatgcacga aacgggtgcg
     1861 tgcatcggca tgggtctgtg acaggtgttc gatcatcggt ctgacgatgt cgccgccggt
     1921 atagtcgaca tcaagcgagg gacgcagcaa ctgcatccag ttgaaatcgc cacatggcgc
     1981 atcgaaaagg cgccgcacac caaattgttt gatgacctct ggcagcatcc ggcggatttg
     2041 ttgtgtggcc tcggctgttg atccgctgcc gctacggctt tccacggatc cccagtagtt
     2101 tagatgatag gtcttggtaa accgtgcctc gatatcctgt tcgttcagaa tggatcggcg
     2161 ctgcaagcgg tttctcgcat tgagaaaacg ttgatgcaac cagctgtgtg actgttccgc
     2221 actgtcggcc atgaaacacc ctgttcgctt ttctgttaag acatgtgacg tttcatttaa
     2281 aataccgcac tccatcaaac taaacaaatg aacatgggga ccagccgccg gcttcttgtt
     2341 gtaaaggtta cccgaggtcc ggccccggtt cgatccggtc ggaaggggaa cccctggggc
     2401 gcgctctctt tttgattgaa tcatcaaaac gggagataac gcggtcgtca tcaatagttt
     2461 ggagcatgct tggctcgact ggagcgcttt cagtgaactc caattcactg aaagcgcttc
     2521 agcggatgaa ctgatcgatg aagccttcgc caagcccgtt ggcgatgtag tgcctgcggc
     2581 agcggtcgat gcgggtgaac acgtcatcga agccggtgca ggtgccgtcc gggtcgcagt
     2641 agatcatcgc gccgttgacc tggaacagcg tgatcatctc gccggcatgg ggatcgacga
     2701 cgagggtgtg ggtctcgccc ggcgcctcgt agacatagcc gccctcgcgc gccacccaat
     2761 cgtgttcgag atagcgccat tcgcccttca gcacatagcc atgcaccggt tgcggatggc
     2821 gatggcgtga gacgacacct gcacggcgca ccctgagcag attgacccaa tagccacgcg
     2881 aagccaccat gcacagcggc cggaaccaga cattgtcatc ggtcggcacc cagacgcgct
     2941 catcctgcgg aatgacgttc ggcaccacca gttcgggcgg gctctccttc ggcatcggat
     3001 ggcgataggg cagccatttg tcggagctcg gcggcacggg gctggtcatg gcattctcct
     3061 tcaaatcaga cagcgaggta accgccatcg accgggacga tcgccccggt gacgaaacgg
     3121 gcggcatcag aggcaaggaa caccacaacc ccgccgatat cggcgggcgt gccccaacgg
     3181 ttcatcggcg tgcgagcgag gatgggtgcc gagcgggcgg catcctcgac cagcggacgg
     3241 gtcagtggtg tgtcgatcca gcccggcgcg atcgcgttga cgcggatccc ctccgctgcc
     3301 caggcggctg caagactctt ggtcagctgg ccaacgccgc ccttggaggc cgagtagccg
     3361 ggcacgaagg gcgaaccctg gaaagtcagc atcgaggcca tgttgacgat ggatccgcct
     3421 gacgtcttga ggagttcctt ggccgtcacg cacatgcgca tcgtgccggt gaggttgacc
     3481 gcgatggttt tctcgaatgc agccagatcg aactcgacgc cgccacgctg gatgatgccg
     3541 gcgcagttga cgagaacatc gagccggtcc atcccccgaa gcgcgtcggc gaccgacgca
     3601 tcgctggtca cgtcgagcac tgtggtcctg atcccggggc ggggcgtcat cgccgcgcat
     3661 tcggcctcgg tcaggccgca ggccagcacc tgaaaccctg cttctgccag cgcccgtgca
     3721 atcccctcgc cgatgccgcg cgttccaccg gtgacaagcg cctgcttcat gtcacccgtc
     3781 ggtccttgcc agggcggccc gcaccttggc cgcatccggg ataggctcca ccgctccgaa
     3841 accttcagtg gaaagcgctg ccgcagcatt ggcatagcgg gccgcatcga aaagatcatc
     3901 gccggccacc aaccgtgcca cgaacgcgcc gccgaacgtg tcgccggccc cggtggcatc
     3961 aaccggcttg cagggcaccg gggcaatgcc gagccgccgt cccttctcgg ccaccagaac
     4021 gccttccgag ccgcgcttca gcgcgacctg ccgtgcgccc ctctccaggc agaaatcgac
     4081 gatcgcatcg gcatcgctga ggccggtgat cgcaacaaca tcatcgtagc tgggcagaca
     4141 gatgtcggac aggccaatga catcggtgat gatggcgcgg gcgcgttcct tgctccacag
     4201 gcgcaggcga aggttggtgt cgaaactgac cttcactccc gcgccccggg cgatttcgac
     4261 cgcccggtag caggcatcgc aggcagaact ggaaatggcc agcgaaatgc cggaaaaatg
     4321 catcaccttg gccctgcgga tggcatcaac cggcaaatcg tccggatgca tgcggctcgc
     4381 ggccgaaccg gaccggaaga aggaaaactg gtgcccgctg gcgtcatggg tgacaaagta
     4441 gatcgcggta aacgccgccg gatcggtggt gacaaagcgg tgatcgaccc gttcggcctc
     4501 ccacagatcg cgcagcatcc ggccataggg atcgtctccc agccggctga catagccgac
     4561 gcgggcgccc tggcgcgctg ccgcgatggc gaaattggag gtgtcgccgc cgaagccctg
     4621 aagataggtc cgggtccctg aaccgcccgt ctggttgaac tcgaccatcg gctcgccgat
     4681 ggccagaatg tcgggatatt cctgcatcat tgcctctcgg gcggatcaga tccggccata
     4741 ttttgccagc ccctttcgca gggacttttc agcaatgatc agttttgcct gttcgagttc
     4801 gcgcacgtca atctcttctg ccatggcaag gacagcttcg gccctggcac gggggaccac
     4861 gacaacgccg tcaatgtcgc cgacgacaat atcgccgggg ctgatcgcca ctccgccgat
     4921 gaatatctcg atattggcgg ccagggtctt gtagcgcccc agtgtggtgc cgggagaaac
     4981 ggcacgcgca aagacgggga aatcgtaatc gcggcggatc tcgacgatgt cgcgcacccc
     5041 gccgtccaga acggccgcct catgcctgtt ggcgatcgcg cctgcggtca tcaggccgcc
     5101 ccataccgcc atctcggctt cgccgccggt cgcaatgcag atcaccgagc cggcttccgc
     5161 ttcgtcgatc agatcgagcg catgctgtgg cggaacgaat tccgtcgtcg gcccttccag
     5221 gatcgtggct gccgggccgc aaatcttctt ttcattgatg cggggcttga ccgcgctgtc
     5281 gagatagccg cggcgcccgc agatcttgtc cacggcatcg gcgacagagg ccgttgcgac
     5341 ccggcggaaa cgcgcgatca gttcgtctgt ggtcatgaga tgctccagca gttaccaggg
     5401 attgatccaa tgtcggggag gtttgccaga cagaatcctg gcgacctcct cggctgcttt
     5461 cttctgcaga tcggcaacgc tttcctcgga ataccaaccg gcatgatcgg cgaggacgac
     5521 gttcgtcatc gtcagcaggg gattgttccg agtcggcggt tcgcattcaa agacgtcgag
     5581 cccggcgccg aaaatcctcc ccgctgaaag ggcctcgacc agcgccacct catcgatcag
     5641 cggcccgcgc gaggtgttga cgatgatcgc ggtcggcttg agcagggcga tccgggcggc
     5701 attcaggata tggcgggtgg ctggtgtcag cggtgcatgc agcgaaacat agtcgctttc
     5761 agcgcacagc ctttccatcg tcaccgcttc ggccgatgcc ggcagatcgg cgaaggggtc
     5821 gtgaaccagc acgcggccaa caccgagcgc acgcatcctg cgctcgaagg cccgggcaat
     5881 ccggccgtag ccgacaagac cgagggtgcg accaaaaggt cggtacatct tttccgacga
     5941 accggtgaac cagccgccgg cccgtaccgc gcgatcccgc gtcacaatac ggcgcgccac
     6001 cgccatcagc agcgcgagcg catgatccga gacatcctca gtgccgtaat cgggcacgtt
     6061 ggccacatag atccggcgtt ccgacgcggc gttccggtcg acgttatcga cgcctatgcc
     6121 gtagcgaacg attgcgcgca ggcgcggcgc cgcagcgatc gccgccgcag agaccggcga
     6181 ttcgcgcacc aggatcgcat cggcatcggc gatggccatc acaacgcgct cggcctgacc
     6241 gcggcagggg cgaagctcca gagtcgctcc ggccgcagcc agaacagcgc gttcggtgtc
     6301 atagctgtcg taaccgtcgt cgacgacgac aacacgcaac ggatgcagtt catgcaccat
     6361 cggaggcatt ccattcggtc agggttggcc ggagcgctag agcactttca gtgaattcca
     6421 attcacaaaa atgctctatc ttgctgtttt ttcgcatttt cttgacgcga accggaatcc
     6481 acttcgcttg aaaatgcgct aagctctccg gccgttatat ccggcgctca ggacttggga
     6541 cggttggcgt cgacagcctt cagcaatgcg tcgacatgct ccttgccgat ttccttgttg
     6601 gcccattcca ccaccggcgg ctgggccagc ttgcggaatt cagcgatctg ggccgccgac
     6661 agctcggtca ccgtcaaacc cttttccgtc aggatgcgct tggcatcctt gtcctgatcg
     6721 ctggtcatct tgcgatggat gtcccgcgca aggactgcgc cttcgcgcac ggccttctgc
     6781 tgatccgccg tcagtttgga aaagaaggca tcattcacca ggtaagcatg gctggaatag
     6841 acatggccgg tgagcgagac gaatttctga tactggaaca gcgaggccgc cagaatattg
     6901 gaaacaccgt tctcctgccc gtcaacgacc ttcgactgca gggcggtggg cagttctgcc
     6961 cacggaatcg ccgaggccga ggcaccgagg ctttccacca atgcgcggaa caccggcgaa
     7021 ggctgaatgc gcatcttcat gcccttcatg tcggccggag agctgattgc cgccttcgaa
     7081 ttggtgaaat ggcgaatgcc gttatcggca aattcaagca gccggatgcc ggtggccttg
     7141 gtcatgccgg cgccgaattc atcgcgccag gcgctgtcat agaccgccca ggcatgggca
     7201 tggctctcga acatgaacgg aatgccgagg attcctaccg ggcgatagac tgtcgaaatc
     7261 gcgccgtcat gggcgaccgc catttcgagc gtcccaagct tgaggccttc catcgttgcg
     7321 gcatcgttgc cgaactggcc tgccggaaag atctggacgc tcaccgagcc cttggtggcc
     7381 ttttcaacat gctccttgaa ggccagggcc gccttgtgtt tcggctgatc atcccgcgcc
     7441 ggctggaaat gcgcgtattt cagcgtcacc tgctgggcaa gggccggcag ggaaaacagt
     7501 gcggcgatgg caaccgagcc tgccagcagc attcggcgat ggattgtcat ggtcatttcc
     7561 tccgtgttga ttggttagcg gctgtagccc agccaggccg ggacggtgag cgagagagcc
     7621 gggaacagga tcagcagcac caagaccgcg atctcggcaa agaccatcgg catgatggcg
     7681 cgggcgatcc gttcaaaacg cagcttcgag accgaggtga tgatgaacag cacaccaccg
     7741 accggcggag tgatcagtcc gatcgtcagg ttgagcacga tcgtcatggc aaaatgcagc
     7801 gggttgatgc cgaagatgtc ggtcagcggc gccagcaccg gcaccagaat gatgacggcg
     7861 ggaagcgtat cgatgaaaaa gccgcagatc agaacgaaga tcgcgaccag aaacatcacg
     7921 atcagcggat cgttggtcag tgtcgcgatc cagctggcaa ccgcctgcgg aacacccaga
     7981 atcgtcagga gccaggcaaa cagcgaggct gtcgaaacaa tgatcagcgc tgcggaggaa
     8041 accagcgccg tttgcaggac tgcccggaca atcaactccg cagtcagctt gcgggtgacg
     8101 aaaaggccga caaacagcgc ataggcgacc gcaacggcag ccgattcggt ggaggtgaag
     8161 ataccggaga caatgccgcc gatgatgatg accggcatca gcagcgccca gagcgccccg
     8221 ccgagcgccc gcaaccgctc gctccagccc ttgcgctcat gcgccccgcc atagccacgg
     8281 cgcaccgaga tgatgtggtt ggtgatagca agcgcgatgc ccagcatgat gccggggacg
     8341 atgccggcgg cgaacaggcc tgctacagtc acgcggctgt cggccaaagc gtagatcacc
     8401 atgatgatcg agggcggaat gatcggcccg atcgtggccg tcgccccgga gagggcggcg
     8461 gcatagtgcg gctcgtagcc ggagcgctgc atcatccgca tcatcaccgc gccaggtcca
     8521 gccgcatcgg caagggccga ccctgaaatg ccggcaaaaa tcatgttggt gagaacgttg
     8581 acatggccaa gtccgccgcg acggtgaccg atcaggctgt tggtgaaggc gagaatgcgg
     8641 ctcgtgatcg ccgatgctga catcaggtcg gcagccagga tgaaaaacgg cactgccagc
     8701 aacggaaagg aattgatgcc gttaaagaag cgctgcgggg cagagatgag cgcaaagcgg
     8761 ccatcgacaa tcagagcggc tacacccgaa acgcccatca ggatggcaat gggcaagcca
     8821 ataagcatgc cgagaacaaa gacgaggccc agtgtggcac ccatggctca gctctcctcg
     8881 ccctgttcaa ggctgcccat ttcctcgtag cgcttctcga caaatgccgg ggctgccatg
     8941 acgagatgga acaggaacag cgcagcgccg aagggaaccg ccgagtaggg aacggcagcg
     9001 gagatctgca atgcaggggt ctgctgctcc gctgcgaaca gagcatatcg cgtgcccagg
     9061 accaccagca gcgccaggaa cacggctgag atcaccagaa caacccagcg gagcatgcgc
     9121 ccggtcgggc cgggcagcgc atcctggagc atctcgacag caacgtgccg cccctggcgg
     9181 aaggccagac cggcgccgag aaacaccatc cagaccatca gatactggga cagttcctcc
     9241 gcccagatca gggatgcgtt gagcacgtag cggaaaaaga cattgccgac gaccagcacc
     9301 accatgagcg cggttatcgc aagaaccaga gcagcgttca gccagacaac ctgccactca
     9361 aggcggcgga gacccgacgg cgggcatgga ggaaagctca tagcggaaga aagcaggtcg
     9421 ggcttgcagt ccgtcatcgt cggcctccct gccgccaaac agaactttga tatctgatat
     9481 atgagatata ttggctcgac aaccccaatc ttcatgctaa cgtcgcatca gacatgcgag
     9541 gttctttttt catgctgaca gtcgttccgg gaccggtgga gcgcccaaag acgttgaccg
     9601 atattgctgc cgcagcgatc cgcgaggcga tcatcaccgg gcgcatcgct cagggggaac
     9661 aggtttccga agcccagctc gccggctcac tgggcttgag caagacgccg gttcgcgaag
     9721 ccctgctgcg gcttcagacc gaggggctgg ttgccgtgtc accgcatcgg gggacgttcg
     9781 tgttctcgct gagtgacggg gagctgactc atatctgcga gttgcgcctg gcgctcgaga
     9841 ccggcgctgt cattctcgcg ctggagcgca accgggagcg gctcgtctcc ggctggcttg
     9901 ccattcttga ccgaatggga gcggcgctga acctgccgga ccccgcggcc tatctggtca
     9961 gcgacagcga gtttcacgaa catctcatcc attgttcggg gaaccgctat tttcaggacg
    10021 cttattcgct gatcgccgcc aaggtcacga ccctaagact caagctcggg cgcgattcgt
    10081 tccatatgga gaagtcgttt ctcgagcaca ggctgctggt cgatcatgtc cgcacggggg
    10141 cggtggagca ggcgcagcag gttttgcggc gccatatcgc ccgcaaggaa ggctcctact
    10201 gggagcagct gaggccggtc aaatcctcct acctggacgc atcgtaaccg agcgatcagc
    10261 ggatcgccgg gggtggcctt cgatcaatcg acgctgacgg cgctcgcaag gcccttgata
    10321 gtgttcgacc aagggcgctg ggcactgata cgatgcggca ggctcggcat gtcttggacg
    10381 ggcagggtgg gaacaggatg agcaagccgg cgctgattgg ggttgattgg ggcaccagtt
    10441 ccctgcgcct ctggcgtttt cgggcggacg gatccgtgag cgataccaag cgatcggacc
    10501 agggcatcat gaatgttggc gaaggccggt ttcgcccggt cttcgaggca gctgccggcg
    10561 cgtggctgga accaggggtg cccattctta tgtgtggcat ggtcggctcg cgccaggggt
    10621 gggcagaggc cagttatgcg ttctgcccgg ctgaggtcgc aaccctggcc gatcatctca
    10681 agccggtgcc aggctatccc aatgccttca ttgtccccgg cgttgcggtg cgggcagccg
    10741 atggccggcg cgacgtgatg cgcggcgagg aaacccagat cctgggcgct gccagtggcc
    10801 caggccggca tctgatcgta ttgcccggca cccacagcaa atgggtgatc gtcgagaatg
    10861 gctgcatccg gacgtttcat accttcatga caggagagct ctacagcctg ttgcggcacc
    10921 attccattct ggcccggctg atgccccaga gcgatgcgga ggtcgatgag gccgccttcg
    10981 agacggccgt gcgcggcgcg ctcgatggtg cggccgcagt gatgcagtca ctgtttgcca
    11041 tcagggcgcg cgggcttttt gccgaattgg cgccggagca ggcggcctca tcgctgtcag
    11101 gattgctgat cggggccgaa ttgcgcgagg ggcttgcttt ggctcgcccg ctcggggtgg
    11161 atcatgtggc gccatgggtc atcggaagct cggcgcttgc gcagcgctac gcccaggcgt
    11221 ttgccgttgc gggcttcagg gtccgaacgg aggatgaagg cgccagcatg cgcggccttt
    11281 gggcgatcgc acggcagagg gggctggtct gattctctca gcgagcaaga cttccggagg
    11341 gcctgatgct ggattttgat ggtgcgatgg cgtctctgcc attgatcgcg gtgttgcggg
    11401 gcctgccgcc ggatgatgcg gtcggtgtcg gtgaggttct ggttgagacg ggcttccggc
    11461 tgatcgaggt tccgctcaat tcacctgagc cgctggtatc gatcggacgg ctggtcaagg
    11521 aattcggctc gatggcggtg atcggggcgg gaacggtgac ttcgcaaacc gatgccaagg
    11581 cggttctcgg cgttggcggc cggctgatcg tgatgccgca ttccgatcct gctgtcatcc
    11641 aatccgccaa ggcaggaggc gggatcgtcg cagccggtgt tgccactccg accgaagcct
    11701 ttgccgcgct tgctgccggg gcggatgcgc tgaagctgtt cccggcggag atgatctcgc
    11761 cggctgcggt agccgcgatg cgtgccgtgc ttgcccgatc ggttcgactt cttccggtcg
    11821 gcggcatcac gccggcgctc atgcctgcct atcacgctgt tggcgtgaac ggctttggcc
    11881 ttggttcggc gctgtacaag ccgggcctgg cgcgcgaggc cgttgccgct cgggcgcagc
    11941 agttcgtgtc tgcctggcag gcgttgacgt ctcgatcgag cggataggca agcgcccggc
    12001 gcagcctcaa tcggccgcaa gcctttttgt cttcgcataa tcttaacgcg aagcggaacc
    12061 catttcgctt gaaaatgctc taataccagt ggccctcaca aatgacctgc cggtcatttt
    12121 ccgggccgtc cctcgcaacc gggccttcgg ccctgctcgg cgtcgcattc gctcctaacc
    12181 agccgcctgc caattgcccc taacggtcaa ttgtcagggc cgctggtata atggtattgg
    12241 cgctgttgtt caggcgacgc atgccgttga tgaagtcagc cggaagctcc agtgaattgg
    12301 gtgattgcag ccccatgaag cccgcgacat cggcgacgaa tgcattgcag ttgtaccaga
    12361 cggcatgcca catcggtgag cttgcctgaa gttggcggat cttggccgag accttgtcgt
    12421 attgcgcggc cgtcaaatag atgcggtacc gggccgaaat atactggtcc tcggtgtcgc
    12481 catcgctcgc ccccgtctcc gaggcaaccg gaatgaaatg accaagcacc caaaccgacg
    12541 ggtcatcgcc ccgcggatgg aggccggcga cttcgcctgt cgcaatccgc ttgccgttca
    12601 gcctgccatg gacaagaaac gtatgcccgt aactatgggc atagcgcgaa cggaactcga
    12661 cgtaatgcgt aagcttggcc tgattgatgc ttgaggtttc cagactgtca tccggctggc
    12721 cgggtgcggt ggccgcattg gcattcgcat aggcctggac gcgcgcctgc tggttggcgg
    12781 gggtggcaca ggccgcaatc ccgaagccca atccggcaaa gatcgcaatc cgtttcagcg
    12841 tctggaatct ggtggcatca accatgatgg ggagcagcct tcaagtgagt ctgcaactcg
    12901 acgcaaaaca gaacgagccg atcaaaaaaa catggcttcg gaatacgccc cgaagggata
    12961 cttctgccca ggcagaggaa gcgggcagcg aagggacggc tgcgcaggat gcgccgccgt
    13021 cccgccgggg atcagccctt ggtgacaatc ggggcagccg gagccttgcc cttgcccttg
    13081 ccaaccgtgc cgcaagcgct gaccgacatg gccaccagaa tcgcgaccgc aaccatcgaa
    13141 agcttcttca tcgcattacc ctcgtcgttg aacttcgcgc cgctctcgca agcgcgatct
    13201 gtgccaccca gatgaagcac agaatcacta taaatctgaa ttgagctaca acgaagtgca
    13261 aaaaagacac agtttgtcga cgttctttgg tatttaaggc gaaaaatcac aaggtcctgt
    13321 agcattataa aaattatatt ctataatatt aattgtatta aatattatca tttgattaac
    13381 aataaagata atataaatta attgccaatg ctatttatga atattgatgt atatgagaac
    13441 cattttatga gtttttgtgt tttttggtct gttccctttg ccagaatcgg gaacgtatct
    13501 attctctttc agagtataaa aatcgctcgc gttggccgaa cggcctcgcc agcaaaaaca
    13561 agatggaaac aacagacgcg ctctgccaca gcgggatgat gctgtcagcg cgtctggaac
    13621 gtaacggagt cataccagtg gcccttacaa atgacctgcc ggtcattttc ccgggccctc
    13681 cctcgcaacc gggccttcgg ccctgctcgg cgtcgcattc gctcctaacc tgcggccctg
    13741 acaattgccc ctaacggtca attgtcaggg ccgctggtat cagctccgtg cggcaccctt
    13801 gatgcccttg tcggccagca tggcctgaag ttcaccagcc tggaacatct cgcgagtgat
    13861 gtcgcaaccg ccgacaaatt cgcccttgat gtaaagttgc gggatggtcg gccagttgga
    13921 ataggccttg atgccttcgc ggatggcgcc gtcctcgagc acgttgatgc ccttgtaggg
    13981 cacgccgagg tagtcgagaa tctgcacgac ctggccggaa aagccgcaca tcggaaactg
    14041 cggcgtgccc ttcatgaaca gcaccacatc attgttggtc acttcagcat cgatacgggc
    14101 attcacatcg ctcatggcgc tctccttgcc gggttcaagg tccggatcga tggagatata
    14161 gcggctcgtg tggtgtttgg gtaggtcgcg ccttgcgcca tcgtagtctg ttcgaatgac
    14221 gtttccggtt cagcagtgtg ttttgatcct gcaaacgact gggggtggga catgtcaatc
    14281 gggcgcggaa ttgcgggtat tgcgggcttg atcgttgccg gttgtgcgac ccatccgctt
    14341 ccggactggc ctacaggcta ttcaacggcg cagattgttc agcacatccg ctgcgaggca
    14401 aggcaagctg ttgcggaagt catgctggaa aaagccggca ttcctgttac gcagagcggg
    14461 gtggacacgt tcacattcgc aacaagggag gacggttcga atgacccact gggcaaactg
    14521 aaagctgcct atcgagacgc tgccatcgcg tttgacttca agctgaccat gaccgaactg
    14581 aacaagttca gtgccggggc aagcatcgcc gacaagttca atcgtggcca cagatcgggt
    14641 ggagcaagca gtggcactct tgtgatgggc gccaagctgg accagacgcg tcagaatgaa
    14701 cgcatgttcg ttgtctcgac caatctggac gatctgtttg aaggtccgga ggcgccaaat
    14761 tgcagagtgc cggcgtctcc cgccaatccg ttttatcctg tcaccggttc catcggcatc
    14821 aaggaagtga tcctgacctt tgtcgaactg gcggaagcct ccgtgctgcg tggcaaggac
    14881 ggagcgggca attcgcgtcg cctcgtcgat acgctgcagt tcaccaccga tacatcggcc
    14941 ggtgttgatt caggcaagct gactatcgcg ccggtggggc tggttctgaa cctcagcgaa
    15001 gtgtctgttg ccggttccgc gggccgtaag gatgttcatc aggtcgggat tgccctgtcg
    15061 ctagcggttg tgcaggctgc gcaagtagtg ccgggcccaa aggcgcacaa aagagccagg
    15121 cagacagagc cgaatcccaa aaagcaggag gcccgtcaga atgcggtgga tggttttgtc
    15181 gagcgcgact accggcggct gcaggatgcg ctgacggtga tccagcgccg actggaatag
    15241 cgggagcgcg ctctcactcc gaccgagtcg tcagcgccag tgcatggagt tcgccgccca
    15301 tgcgcccctt cagcgcggca tagaccatct gatgctgggc gacgcgcgat ttgcctttga
    15361 aggccggggc gatgacggtg gcggcataat gttcgccatc gccggcgagg tcacggattt
    15421 ccacggtcgc atcgggaaag gcttcgcgga tcagcgcttc gatatcggca gctttcatgg
    15481 ccatggggcg gttccttatg cctgtccgct catgaaggcg ggcagccagt gttcgtgggc
    15541 tttggatagt tgggcaaccg acagcggggc gcagcccggc agggtgagcg tggcgccacc
    15601 ggtggttccg agcagcaggg ctgcaatgcc ggccttgccc gcttctgcga tcagcgcctc
    15661 ggcctttgcc gccggcactg agatgacata acgcgcctga tcctcgccga acaatgcggc
    15721 caccggggca attccggccg gcaggcgctc gatcgaggcg cccagaccgc cggccatcgc
    15781 catttccgcc agcgcgatgg cgaggccgcc gtccgacaga tcatggcagg cgctgacgcg
    15841 gcctgcgagg atgagcgagc gaacgaaatc gccattgcgc ttttcaatgg ccagatcgac
    15901 cggcggcggg gcgccgtcct cgcgaccgag aatggtggcg agataggccg aggccgccag
    15961 ccagccatct gtccggccga tcagcacgat ggcctcgccg ggtctggcaa agccgatccc
    16021 gaccgattgc gtgacatcct tgatcaggcc gacgccgccg atggtcgggg tcggcaggat
    16081 cgccttgcct tgcgtctcgt tgtagagcga gacgttgccg gaaacgaccg ggaaatcgag
    16141 cgcgcgcgcc gcttcgccga tgcctttgat ggcaagggcg aactgaccca tgatctcggg
    16201 cttttccgga ttgccgaaat tgaggttgtc ggtcagcgcg atcggcgtgg agccggtggc
    16261 ggtcaggttg cgccagcatt cggcgaccgc ctgcttgccg ccctcgaacg gatcggcctc
    16321 gacatagcgc ggggtcacat cgcaggtcag cgcgagcccc tttggcccct cgccgacgcg
    16381 gacgatcgcc gcatcaccgc cggggcgctg cacggtattg ccgagaatga tgtggtcgta
    16441 ttgttcccac acccagcgcc tggagcagaa atcgggtgag ccgagcagct tgagaagcgc
    16501 ggcggcatgg tccgctgcct tggcctcggc cacgctgaca attccgttga tcagcgcgga
    16561 attgcctggg cgcggcgccg gcgccaccca ggggcggttg tattcgggtg cctggtcgcc
    16621 aagttccttg atcggcatgt cggcctttac ctcgccttcg tgcttgatga cgaagcgcag
    16681 ggtatcggtg gtgtggccga caatggcgaa atccaggccc catttgcgga aaacgtcctc
    16741 ggcttccttc tccttttccg gatgcagcac catgagcatg cgctcctggc tttccgacag
    16801 catcatttca taggcggtca tccgctcctc gcggcagggc acgttgtcga gattgagttc
    16861 gacaccgaga tcgcccttgg cgcccatttc caccgccgaa caggtcaggc cggccgcacc
    16921 catatcctgg atggcgacga cgcagccctt ggccatgatt tcaagacagg cttcgagcag
    16981 cagcttttcg gcgaagggat caccgacctg cactgtgggg cgcttttcct cggaattctc
    17041 gtcgaattcg gccgaagcca tggtggcgcc gtggataccg tcacgcccgg tcttggagcc
    17101 gagatagacg atcggcaggc cgaccccggt cgccatcgag gtgaagatca ggtcgcgctt
    17161 ggcaattcca accgccatgg cattgaccag gcaattgccg ttgtagcgag ggtggaaccc
    17221 aacctggccg ccaaccgtcg gcacaccgaa ggaattgcca tagccgccga tgccggcgac
    17281 gacgccgccg accagatgcc gggtcttggg gtgcgaggga tcgccgaagc gcagggcatt
    17341 gaggcaggcg atcgggcggg cgcccatggt gaagacatcg cgcaggatgc cgccgacgcc
    17401 ggtcgtggcc ccctgatagg gctcgatata ggacggatga ttatgggatt ccatcttgaa
    17461 aacaacgaca tcgccatcgt cgatatcgac cacgccggca ttctcgccag gtccgtggac
    17521 gacgcgctca cccttggtcg gcaaggtttt cagatgcacc ttcgaggatt tgtaggagca
    17581 atgttcgttc cacattgccg agacgatgcc gagttcggtg atcgtcggtt cgcgtcccag
    17641 cagtttgagg aaacgctggt attcgtcggc cttcagcccg tgctgggcga cgagttcagg
    17701 cgtgatcttg atgtcgttcg ggatcattgc ggcgggccat gtgtcgatgt ggcgccttca
    17761 taccgataga gcggtcgtct cgccacactt tcttgtcagc cgccaccggt aatgccgtcg
    17821 cgcgctttgg ccacccggta gaaggcccag agcaggcggg cagcgaccgc gcgccagggg
    17881 cgccagcctt cggccagaac ggccagttcc ctggcggagg ggcgggcgtc aaagcccaaa
    17941 gccaggcgcg ccgcctcctg cagggcaaga tcacctgcgg cgaacacatc ggcgtggccg
    18001 gcatagaaca tcagatagat ctcggcggtc caggggccga tgcccggaac agaggtcagc
    18061 gcggtcatcg cctcctcgcc cggcatgtcg cacaatgcat cgaccggcag ggtgccggat
    18121 cgcaccgcct cggcaagccc ccgcagtgtg cgcattttcg gcgtggagag gccaaaggcg
    18181 cgcaaagtcg cttcatcagc gccgagaatc gcctccgccg tgatctcgcc gaggccagct
    18241 tttgtcttgc gccagattgc atcggcgctg gccaccgaca attgctggct gacaatgatg
    18301 ctgcaaaggc cggcaaggcc aggaacatga cgcctcagcg gaatggggcc ggcaatggcg
    18361 gcaacgcggg caaggcgcgg atcggccttg ccgagatggg ccattccctc ttcaatgtcg
    18421 gcgtcagtcc ggatgatgcg catcaggaca ggaaaggcat acgagccccg ctttgcaacc
    18481 cgttttccgt tttgctccca gccccaacgg tttccttcat ctcggccatg cctattcggc
    18541 gctgctcaac cagcatctgg cgcgtgaatc gggggggctg ctgctgctca ggatcgagga
    18601 catcgatacc acccggtgcc ggccggagct gctcgaagcg atgttcgagg atcttgaatg
    18661 gctggggctg gagttcgatc agccggtgct gcggcaatcg accgaatttg cacggtatcg
    18721 ggctgtgctg gagcgtctgg aggctgatgg tctggtctat ccctgtttct gctcgcgcaa
    18781 ggatattgcg gcggcggcgg gcgatcatcc cgtgcgcgat ccggacggtg cgccgctcta
    18841 tgccggcacc tgccggcggc tgagcaatgc acagatcagg cagaagcgat cagagggcgg
    18901 ggcttttgcg ctccggctcg acatggcggc ggcgctggcc cggatcggca cgcggccgct
    18961 cgactggcag gaagccgggc agggacgcat cgcggcggag ccggcgctgt ggggggatgt
    19021 ggttctgggg cgcaaggagg tgccgaccag ttatcacctt tccgtcgtgc tcgacgatgc
    19081 gatgcagggc gtcaccgata tcgtgcgcgg gcgcgatctc ttttccgcaa catcaatcca
    19141 ccgcctgttg caggtcctgc tggatctgcc ggagccgcgc tatcggcacc atcggttgct
    19201 gaccgattcg gatggtctga aactggccaa gagccgttcg tcgcctgcct tgcgccagtt
    19261 gcgcagcgag ggtgtgagcg ctgccgatct gcgcctgcat ctgggatttg cgcctatctg
    19321 accagccaga cccagaaggt cgaggtgacc acggacagaa ccgtcgagac cgcaattgcc
    19381 cccgatgtga ggcccagtcc aagccggtag cgctcggcca acaggtaggc gttgatcccg
    19441 caaggggccg cggcgatcat ggtgatgacg cccgcccaga ccggcggaag cgcaaagagc
    19501 ttgtacgcca gaagatagac gatggccgga aacaccaaaa gtttcagcgc caccaccagc
    19561 gccagcagcc ggtagtcccc gcccagacca tagcggctca gggccgcgcc catagcaaac
    19621 agtgcgcagg gtgccgaggc atcgccgatg aacttcagac tcgacatgac cgggtcagga
    19681 atcagccagc cggtctggcg ccacaacagc ccggcggcaa tgccgatgag gatcgggttg
    19741 gtcgcaagct tgaccagcat ggcccggtat cgcccggcgg gcgcgtccga ggtctcgatc
    19801 agcaccgtcg cgacggtcag ggtgatgggc aggttgaccg ccaccatcag gaacagcggt
    19861 acgctgcctt cctcgccgaa tacgcgcagg accatgggaa tcgagataag caccagattt
    19921 gactggccga cggaaaagcc aatgatgctg gcttcacgat tgtcgcgttt catggcgcgg
    19981 cgggcgacct gtgccacgat tgtccagacg atcgccatcg ccagataata gcttagccag
    20041 tagctccagg gcgggctcgc cggcaggggc gcggtcgcga tggtgcggaa cagcatcagc
    20101 ggaatggcga tgacgaacac gaattccgaa agaccgttac cggattctac tcccacaaac
    20161 cgcaggcgca ccgccagcca gccgagcaga atcactgcga aaatcgggaa gaggatgatg
    20221 aaggtctcga tcatgtcggc ttcccttacg gcacttgtct tgcattttac cctttggttt
    20281 ggccccgact taaaccgctg ccgttagagc tttgccatcg agttcgcacc agccaggtgc
    20341 caatacgcag gggacaggat tgatggcggg tcaaatcagt aacgctgctg caatgcaagg
    20401 ccaacagatc gaacggctga gggatctggc gcatgatctg cggtcaccgc tcggcggcat
    20461 cgattcgatg atcgagctgc tcgccaaaac gcggattggg ccggaccagg atgtgctgat
    20521 ccaggggttg caggctgcct cggcccatct cagggcagtt gccggggcga tgctcgcgcc
    20581 ggccgaggtt ccgggccagg aagcgaccac gccattgatg caggttctga ggacgttttc
    20641 gccttcggcc cgagcacggt cgcgcgtgcg cgagcaatat tatgcggaag cgatcgatcc
    20701 gtcctgccgg accattctca tcggcgacgc aactgcgttg cggcaggttc tggaaaacct
    20761 ggttgacaat gcctttcggg tcgcaccggg cggtccggtc agtcttgcgg tcggcgaggc
    20821 catccggccc gacgggatgg ccgtcgttcg actggcgttg agtgacaagg ggccgggcct
    20881 gaccgaggcg gaagccgcgg tggtattcga gcgcgggaca accctggccg accggcaggc
    20941 cgggaccggc ctcgggcttg ccatcgtccg gcggctcgtc gaacaggcgg ggggcagctg
    21001 tggcgcatcc tctcccggca agggccaggg cgcgacattc tgggttgaat ggccggtggc
    21061 cggccggcag caggaatttg ccgaatcgtc tccgatgccg gcgcaaaccg acggagggcc
    21121 gcagatcgtc atcgtcgatg acgatgcgac cagccggaac ctgctcaaac tcctgctgga
    21181 tcattttgaa ctgcccgctg ttgcgctggc ctcgccgctg gaggccctcc gggtgatcga
    21241 gagccagcgg ccagctgcaa tcctgactga catgtcgatg ccgcagatgg acgggctggc
    21301 gtttgtcgag gccgtgcggg gattgttcga cgttgaaagc tgcccgccga ttatcgcggt
    21361 gaccgggcgc gtccatcccg aggaccagaa ggcgatgcgt gaggcgggct gtgcggcgat
    21421 tgtcgaaaaa ccgctgacca tccacgatct caggcaggcg ctgaaggcga cgggtctgac
    21481 cggcagcgga tcgacggtga aagccgcgtg atcagggccg cgcagaggcc tggccagctg
    21541 ccgtaaggct ggcggcgctg ccaggcgcct tcttgagcac ctgatcggtc tgttcgcgct
    21601 ggcggcggaa atccgccagc tgagggccat tgagctcgcg gccgcgcggc agacgaatcg
    21661 ccagcggatc gacataacgc tcgtcgatca tgacttcgta gtgcaggtgc gggccggtcg
    21721 acaggccgga tgagcccaga tagccgatga cctggccctg acggacgcga acgccttcgg
    21781 cgatgccgcg cccgaaggcg gacatgtgac tgtaagtggt gacatagcca ttggtgtgct
    21841 gaatctccac ccggcggcca taacccgaat cccaggcggc gaaacggaca gtgccgttgc
    21901 cggcggcgag aatcggagtg ccgatcttgg cggcccaatc aacgccgttg tgctgacgcc
    21961 aatagccaag aatcggatgc cggcgactgc caaaagtcga gcgaagctcg ccatcggtga
    22021 tcggcttgcg gatcaggaat ttcttggccg accggccctg atcgtcgaaa tagtcgacaa
    22081 cgtcatcatc aggcagggcg aagcgataga atttacgcgt ttcgccgccg aaggtcagac
    22141 tgagcatcat cagttcgccg ggggtatcgg aatcgtcgtt gccgatcagc acctcaatgg
    22201 aatcgtttga gccgactgct cgttgcaagt cgacatcgaa gaacagaacc cggacgattt
    22261 cgtcgatcat cggcggtgga atgccgtgct tgcgcgcggt ctcatgcagg ctgctgaaca
    22321 ggctgaagcc gccttcgccc tcatcgtcct cgccggtcgt gcgccggctc ggcgctgcag
    22381 gacgcgtggc ttcgatcagc acgaagctgc cgtcatcctt gcgaccggcg gcggcggcac
    22441 ggatctcttc gttatagaga tcaacccgga caaccgattt ggccgccccc ggctcaagcg
    22501 cctgaagtgt cagtttgagc cgcttggagg tgtccagcac ctgcccaccg cgggtcaggc
    22561 tgcgcacgat gtcgcgtgcc tgggacggtg tcagcccggc ggcgcgaagg acctgttcgg
    22621 cattatcctc cgagcggatg ccgaccagcc tctgctcctg cgcattgcgc gcgagcgccg
    22681 cctcgagacg gggggcgacc gtgacgtttt ccggcaccat gcggacgacc aggcgggaaa
    22741 acggatcatt ggttccagca aagggcagcg atgaggtctg ttccggtgcg cgcatagcct
    22801 tggccagaag catctgcgcc ggtagggtgg gcgcggtgcg gcgggttgcg gacagggcct
    22861 caagcacctg cgcctgcact tccacttcgg acagactggt caggaacagg gcagggcgat
    22921 cgccatcctc gatcgtgcga gtggacagcg aaacgtctga atcaagcggc atgccctggg
    22981 cagcctcaag cgcgcggtcc tcgctggcgt ccgcaatcag ccgtgcggta ttgaaggccg
    23041 gaatgacatt ggcaaaaccc agtgtttcca tcgccaatgg gctggaaatc cgggtgaagc
    23101 tggccggacg aatcacatca ccgccagcag tcttgcgcac caccggcgtc ttgtaggtct
    23161 gacgtgcggc aacgatgtcg gccttctgca ccaaccggtc gccctttcgg gcagcgatga
    23221 ccagttcgcc ggggagttgg gaggcgatct cgcgcggctt gatcatatgg ggcagttcga
    23281 caatcgcgcc ccggtgctcg atggcgctga tcgcggccgc gagcatcaat cccatcccgg
    23341 caaaccctgt cagcaccgtt ccggcgagcc agcgggccga gatccggccc ttcggcggcg
    23401 gttcaccaga acgattgaac agcgccaacg gaggctcgac gccgaggtcg atcgtgctgt
    23461 ccgggatata gacctgcgtc cctgacacgt tagtgccctg cctcccacct tgctgcgcag
    23521 cgtcgcgcga tttgtcttgc caatacgtca agctcgaatg actgccgctt tgccctgtct
    23581 atcaaggccg gacgcaggcg tccacgcgcg aggagctggt tcaaccatgg cgatggcaaa
    23641 gttgtggcga acaatgcgcc gaacgcccgc gcactctgcc gtgcgcgttc agtgcgcgtg
    23701 ttgtgcagac gcgaagggag atgggccatt ttggtgcatc gcgaaaaaaa tgcccggttt
    23761 tcaaaaaagg ccgttgacag ggagaaaagc ttggggctat acagccgcca tgttgacgcg
    23821 ctgcttcggc ggcgcgggct tcgcttcctc tcgaaagacg agttggtccc tgacctggct
    23881 ctgctgggaa agaaaagcgt ggcttcgctg tttggcaatt gaagacgaga aagagaaacg
    23941 tggacggcga tgttcttgcg gaccgatcct aaccgatcgg tcatacgaga cttcggcggt
    24001 ctaacgtttc aggtgagtgt acacacgctc gacgtcgaaa gatgtcgatg agtgcactcc
    24061 gtcaatgaac gttgtgatca gccggttcaa atctcgagtc caacttgaga gtttgatcct
    24121 ggctcagaac gaacgctggc ggcaggctta aca
//
LOCUS       DAOMXG010000015        24102 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_1301_length_24102_cov_11.089783, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000015 DAOMXG010000000
VERSION     DAOMXG010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 24102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24102
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1301_length_24102_cov_11.089783"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1758)
                     /locus_tag="PLQ11_02120"
     CDS             complement(<1..1758)
                     /locus_tag="PLQ11_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018264527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HRK23727.1"
                     /translation="MAPPRRERSGRSAEPRGKKRPPTRRRGFLRGLVYWSAVASVWCA
                     IGLAGLIGYYALRLPPIDQLAVPKRPPNIAILAADGSLMANRGETGGSNVAIGELPPY
                     VPKAFVAIEDRRFYEHFGLDVVGLGRAFMRNVTARRLEQGGSTLTQQLAKNIFLTQER
                     SIARKAQEAILALWLERSYSKDQILELYLNRVYFGSGAYGIEAAAQRYFGKTARLLTV
                     AESAMLAGLMKSPSRLAPNRNPEAARERASLVLKEMREQAHISEQQHKVALSAPAEAR
                     RNHGAGSVNYAADWVMDMLDDYVGTIGEDVQVTTTFMMPVQNAAERALTAALDAQGAD
                     KNVGQGAIVAMAPDGAVRALVGGANYADSQFNRAVLARRQPGSAFKPFVYLAALDAGL
                     TPETVREDAPVNIKGWQPENYTREYFGPVSLTRALSLSLNTIAVKLGQEVGTKAVIRT
                     AQRLGINAPLAANASLALGTSEVSPLELTTAYAAFANGGIGVAPYGIRRVRTASGKVI
                     YERKEHNRGRVIEPSVLAYLNGMMRETLVNGTARKAELPGWLAAGKTGTTQEHKDAWF
                     VGYTGALVATVWLGNDDGEP"
     gene            complement(2120..3295)
                     /locus_tag="PLQ11_02125"
     CDS             complement(2120..3295)
                     /locus_tag="PLQ11_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HRK23728.1"
                     /translation="MTDAPLAGLRVLELARILAGPWAGQTLADLGADVVKVERKGAGD
                     DTRAWGPPFVPARDGGHLSAAYFHSCNRGKRSIEADFDNPDDIAMICRLAARADVVIE
                     NFKVGGLNKYGLDAESLMALNPRLIYCSITGFGQDGPYAPRAGYDFLIQGMGGAMSIT
                     GDAEGQPTKTGFAIADIFTGLYASVAILAALNRRRDTGAGAVIDCALLDTQVAVNANQ
                     AMNYLVSGKVPGRMGNAHPNLVPYEVFPVADGHIIIATGNDGQYRKLMNVLGQPELGS
                     HPDYIDNASRIANRSALIPLIGSLSLRFAKADLLARLEAAGVPAGPINAMDEVFADPQ
                     VIHRGMRVDLVEDQAAAGTIPSVRSPILIDGAPQVSGRASPALGAHTAEVLADPDWT"
     gene            complement(3299..4105)
                     /gene="dapB"
                     /locus_tag="PLQ11_02130"
     CDS             complement(3299..4105)
                     /gene="dapB"
                     /locus_tag="PLQ11_02130"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HRK23729.1"
                     /translation="MMVRVVIAGATGWVGKALVQAVDAAPDLTLVAAVSRSAAGKDAG
                     MMAGGAANGVTIVATLDEALAAPSDVVIDYTKPQVVKGHTFTALKAGRGVVIGTSGLG
                     ADDYAEIDAAAMSARLGVLAAGNFSITATLLRRFALEAAKYVPDVEVIDYASAKKPDS
                     PSGTGRELAELLGQVRMDGTSVPVDKVGGIPATRGAAVGGPIPVQVHSVRMPSFVLSC
                     EAIFGAPDERLVIRHDAGSSAAPYVAGTLLAARKVGGFRGLRRGLDHVLD"
     gene            complement(4102..5295)
                     /locus_tag="PLQ11_02135"
     CDS             complement(4102..5295)
                     /locus_tag="PLQ11_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRK23730.1"
                     /translation="MAAVTKAAFDWSDPFLLDDQLNEDERMIRDLARSYAQDKLMPRV
                     RAAYAQERTDPAIFREMGELGLLGITVAEQYGGAGASYVSYGLVAREVERVDSGYRSM
                     MSVQSSLVMYPIEAYGSEAQKKKYLPRLASGEWIGCFGLTEPDAGSDPGGMRTRAVKT
                     AGGYRLNGAKMWISNSPIADVFVVWAKSEAHNDEIRGFVLEKGMKGLSAPKIEGKLSL
                     RASITGEIVLDNVEVGEEALLPHASGLKGPFGCLNRARYGISWGAMGAAEFCFHAARQ
                     YGLDRRQFGRPLANTQLYQKKLADMLTEISLGLQASLRVGRMFDAGTLAPEAISIVKR
                     NNCGKALDIARAARDMHGGNGISEEFHVMRHAANLETVNTYEGTHDVHALILGRAITG
                     LQAFF"
     gene            complement(5377..6903)
                     /locus_tag="PLQ11_02140"
     CDS             complement(5377..6903)
                     /locus_tag="PLQ11_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012649969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HRK23731.1"
                     /translation="MTNRPAPRVLRLNAADNVVIAVDQIDLGSAVNGVTATARIPRGH
                     KMAVSRIEKDQPIRKFGQIIGFASEAVLPGAWVHEHNCYFAAFDRDYAFCADARDEPV
                     LALDRRATFEGYRRDSGKVGTRNYIGILTSVNCSATVARFMAEEVMRSGLLNEYPGID
                     GVIPLVHGLGCAMSTKGEGYEVLKRTQWGYAANPNMAGVVMVGLGCEAFQIQRWKEAY
                     GLKESDTFRSFTIQEVGGTRKTIAAGVDAIRTMLPIANRARRQTCPASDLMLALQCGG
                     SDGYSGITANPALGVASDLLVAHGGTTILSETPEIYGAEHLLTRRARTREIGEKLVEI
                     IRWWEDYTERNRMDMNNNPSPGNKAGGLTTILEKSLGAAAKGGTATMNGVFRYAEPVT
                     VKGFAFMDTPGYDPVGVTGQVAGGANVLCFTTGRGSAFGCKPTPSIKLATNTPIYEKM
                     VDDMDINCGDVLDGVSLEAKGREIFDRILAVASGERSKSEQLGYGDAEFVPWQIGATM
                     "
     gene            7031..8200
                     /locus_tag="PLQ11_02145"
     CDS             7031..8200
                     /locus_tag="PLQ11_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRK23732.1"
                     /translation="MTLSLTPEATLPVDGTAGTLVARVLVPDVGPCIAAVRAEGVFDV
                     TAAFPTLRDLCEADDPAGALWAEKGLRIASLSAILANTPPKDAAKPHLIAPIDLQAVK
                     AAGVTFAVSMLERVIEERARGNPAAAAEIRKEVTRLVGDDLSRLKPGSPEAMRLKQVL
                     IAQGAWSQYLEVGIGPDAEIFTKAQTLSSVGTGFEAGLHPMSQWNNPEPEVVLVVSSR
                     GRIVGATLGNDVNLRDVEGRSALLLSKAKDNNASCAVGPFVRLFDATFSLDDVRQTEV
                     TLRVEGRDGFVLNGASNVAKISRDPADLVKQMIGRHHQYPDGAVLFLGTMFAPTKDRD
                     GEGKGFTHKLGDIVTIAAPKLGRLTNVMTHSDKAPEWTFGVGALMRNLARRGLLS"
     gene            complement(8216..8722)
                     /locus_tag="PLQ11_02150"
     CDS             complement(8216..8722)
                     /locus_tag="PLQ11_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018264235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRK23733.1"
                     /translation="MITPDYLRMMARYNRWQNRNLYAAAGALADAQRRADRGAFFGSL
                     HGTLSHLYWGDCIWMHRFSGSPKPSGTIADSPRFRPVWDDLVHDRDALDAAILAWAEV
                     VDPAWLAGETRWFSAAAGRELAQLNARLVVHFFNHQTHHRGQAHAILTGLGARPADTD
                     LILMPDIV"
     gene            complement(8811..10043)
                     /locus_tag="PLQ11_02155"
     CDS             complement(8811..10043)
                     /locus_tag="PLQ11_02155"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008550601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRK23734.1"
                     /translation="MPVLLPILSLVESIKGIAGMFDVVVAGGAVMGSSIACHLAGQSG
                     FAGSILVVERDRSYRQAASALSASSIRQQFSQPVNIRISMHGIAFLRDIGNLLEVDGA
                     RPDIGLREGGYLYLATVPGMAVLSANHILQAREGADIALMDRAALSVRFPFLATEDLA
                     GGSFGRSGEGWFDGYQLMQAFAAKARSLGVTYRQAGVVAVERQGPRIVAVHLDDGSRI
                     PCGAFVNAAGASGARALAAACDVPIPVYAKKRSVFSWSCRERLDGFPLLIDTSGVWCR
                     PEGEGFIGGYSPADDSETDEGQDFEVNWNEFDEVIWPNLAARIPAFEAIRPGRAWAGH
                     YDMNLLDHNAIVGRLGDLENAYIAAGFSGHGLQQAPAVGRGLAELIATGAYRTLDLAD
                     LSFERIVAGRPLRETNVI"
     gene            10118..11647
                     /locus_tag="PLQ11_02160"
     CDS             10118..11647
                     /locus_tag="PLQ11_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRK23735.1"
                     /translation="MPASLADETLGLLKRLGVAEGRLAKSGLAARSPISGETLCHVRT
                     NSSGETTAAIGRAEAAFRLWRSVPAPKRGEFVRLLGEELRAAKADLGLLVTIEAGKIR
                     SEGLGEVQEMIDICDYAVGLSRQLCGLTIATERANHRMMETWHPLGVVGVITAFNFPV
                     AVWAWNAALAFVCGDSVVWKPSEKTPLTALAVQAIVARAAARFQAAGNGMVPQGLSEV
                     IIGGPETGQTLVDDSRVPLVSATGSTAMGRKVGPRLAQRFARSLLELGGNNAAIVAPS
                     ADLDLALRGIAFAAIGTAGQRCTTLRRLIVHESVYDRLIARLIKVYDSVRVGDPREAG
                     VLVGPLIDRSALDSMQTALKDARAAGGRVHGGVPVRSLAGAYVKPALVEMPAQCGPML
                     HETFAPILYVVKYRDFDAAIALQNDVAAGLSSSLFTLDMREAERFVAATGSDCGIANV
                     NIGPSGAEIGGAFGGEKETGGGREAGSDSWKAYMRRATNTINFGTDLPLAQGVSFDID
                     D"
     gene            11813..13135
                     /gene="ugpB"
                     /locus_tag="PLQ11_02165"
     CDS             11813..13135
                     /gene="ugpB"
                     /locus_tag="PLQ11_02165"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HRK23736.1"
                     /translation="MSLIRTAAFLAATALTAVAAPAMAQTEIQWWHAMTGANNDVINK
                     LAEDFNASQKDYKVVVSYKGSYADTMNAGIAAYRAGNAPHIMQVFEVGTASMMAAGKA
                     IKPVHELMKEAGEAFDPASYLPSVAGYYSTVKGEMLSFPFNSSSAVMWYNKDAFAKAG
                     LNPDAPPATWPEVFEAAKKLQGAGYASCGFSTAWITWVNIEQFSIWHNIPIGTKANGI
                     DGFDTEFKINSPVHVRHWANLAELQKTKTFDYSGRTNTGEGRFTAGECPIFLTSSAFL
                     GNVRANAKFAWANAPMPYYPDVAGAPQNSTLGGASLWVMGGKAANEYKGVAKFFTFLS
                     DTDRQAKLHTESGYLPITKAAYEKVKASGYYKDNPYNETPLKELTNKPPTENSRGLRF
                     GNLVQIRDIWAEEVEEVLTGKKTPQAGADAMVSRGNAVLRQFERTVSR"
     gene            13220..14101
                     /gene="ugpA"
                     /locus_tag="PLQ11_02170"
     CDS             13220..14101
                     /gene="ugpA"
                     /locus_tag="PLQ11_02170"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HRK23737.1"
                     /translation="MDKRTVFSGYAIPAALLLPQLLITVIFFYLPAGQAVWQSFQLED
                     AFGTSRQFVGFENYQILFSKPEYFKAMGVTLVFSSAVAFLSLSISLLLATMANKHIRG
                     AGAYKTLLIWPYAVAPAVAGVLWLFMFQPSLGMVAQLLKRWGIDWNPLLNGNHALMLI
                     ILAATWKQISYNFLFFLAGLQSIPHSLIEAAAIDGARPVRRFWTVIFPLLSPTTFFLL
                     VVNITYVFFDTFGIIDAVTGGGPAASTTTLVFKVYHDGKSGGDLGLSAAQSVILMVIV
                     VALTAIQFRYIERKVNY"
     gene            14101..14952
                     /gene="ugpE"
                     /locus_tag="PLQ11_02175"
     CDS             14101..14952
                     /gene="ugpE"
                     /locus_tag="PLQ11_02175"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018480638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HRK23738.1"
                     /translation="MVERRSFLNVALPHIILMLGVVIVFFPVYLAFVASTWDPATIIN
                     GNMGVLPGPHLIENYNRILFSGTSSSTREPVINMMFNSLCMAVMIAVGKIIISIFSAY
                     AIVYYRFPFRMAAFWIIFVTLMLPVEVRIYPTYKIVSDLGLLDTYPGLTIPLIASATG
                     TLLFRQFFMTIPDELLEASKIDAAGPFRFFKDTVLPLSMTTIAALFVIQFIYGWNQYL
                     WPLLITTKDSMATIVIGIKKMIVTQDALTEWQLAMATAVLALLPPVIVVISMQKLFVK
                     GLVETEK"
     gene            15067..16128
                     /locus_tag="PLQ11_02180"
     CDS             15067..16128
                     /locus_tag="PLQ11_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
                     /protein_id="HRK23739.1"
                     /translation="MADVTLKDIRKVYPGGVEAVKDIDFAIPDGGFCVLVGPSGCGKS
                     TLLRMIAGLETITDGECRIGGRIVNDVEPADRDIAMVFQNYALYPHMTVYNNMAYGLR
                     NRKTPEDEIARRVAEAARILAIEPFLDRKPRALSGGQRQRVAMGRAIVRKPQVFLFDE
                     PLSNLDAKLRVHMRVEIKKLHNSLKVTSIYVTHDQVEAMTLADMIVVMSAGRIEQIGT
                     PADVYSRPESVFVATFIGSPAMNLVKGEVVADGQVSLAGTRLACPTGAAAAGTEVLIG
                     VRPDEVTLDPTGPFHSAFTFIEELGPSRLYHGQIAGQDFVVQAPHHALSSTVDSLHFA
                     IDVRAVHLFEAKSGKRIMT"
     gene            16125..16475
                     /locus_tag="PLQ11_02185"
     CDS             16125..16475
                     /locus_tag="PLQ11_02185"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - diacylglycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HRK23740.1"
                     /translation="MKRLLSAFRNSMAGLGWAIRHEAAVRQEAILLAIGVPLAFLLTG
                     DGATRLMLIGGLALLMAVELLNTAIEKLADFITLDRDPRIGLVKDLGSAAVFVLIVLN
                     GTIWAWAVLSWLLR"
     gene            complement(16493..17554)
                     /locus_tag="PLQ11_02190"
     CDS             complement(16493..17554)
                     /locus_tag="PLQ11_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRK23741.1"
                     /translation="MAGAYGLYSHIQANRRRSVILIVGLFLLVYAMVFAGALVVESFG
                     PAPTLDRMLRQSLDNTLSALPLATIGTAVWIAIAYRFHQTMIDSLTDSHTVSRAEAPE
                     LYNLLENLCISRGLAMPQLKIMEADVLNAFASGMSEAQYAVTVTRGLLKELDRNELEA
                     VLAHELTHIRNGDVRLMVVAVIIAGVIGFFGELAFRWATYSPRVVFPDESPRARKTSP
                     AGTALFIAGMIIFLAWLASGLLRFALSRSREYLADAGAVELTKNPDAMISALMKISGR
                     AELPGAPSGLMEMCIENEHAGFFDLFATHPPIDARIAALVDHAGGQKPTDAPPQPSFT
                     RDAVHLDRRLAPWGRQTGA"
     gene            complement(17577..18143)
                     /locus_tag="PLQ11_02195"
     CDS             complement(17577..18143)
                     /locus_tag="PLQ11_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HRK23742.1"
                     /translation="MSAGWVILGILVLLVLWGIGIYNGLVNMRQRAKQAFADIDVQLK
                     QRHDLIPNLVETVKGYAGHEKETLQAVINARNAAISAQGPAAQAQAEAGLSGALGRLI
                     ALTEAYPDLKANANFQQLQGELSDIENKLAAARRFFNNAVAEFNAGIENFPAVLIASR
                     FGFSAMSMFDVGEETRKTLDATPPQVKF"
     gene            complement(18252..18719)
                     /locus_tag="PLQ11_02200"
     CDS             complement(18252..18719)
                     /locus_tag="PLQ11_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009467793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcB family DNA repair protein"
                     /protein_id="HRK23743.1"
                     /translation="MSGAETEALEDGRQSPAALRIARGTRRFLRNLDMTTLTEVPLRS
                     NRRADLVAVGPKGEIWIIEIKSSVADFRADTKWPDYRQHCDRFFFATALDVPVAIFPS
                     DAGLIIGDGFSAAVEWEAPLVPLASATRKELLVRLGRLAADRLHALQDPALPL"
     gene            complement(18716..20338)
                     /locus_tag="PLQ11_02205"
     CDS             complement(18716..20338)
                     /locus_tag="PLQ11_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HRK23744.1"
                     /translation="MTAGQQADETFDYIVIGAGSAGSILGNRLSADGKRVLVLEAGGK
                     DNWIWFHIPVGYLFAIGNPRSDWCLRTTRQPGLNGRDIAYPRGKAIGGSSAINAMLYL
                     RGQAADYDGWRQLGLAGWGWDDVLPIFMGHEGHAGGANAFHNDRGPLRVDFARARWEI
                     LDAWRDAAATEGIPKTDDFNRGDNLGSSYFQLTQNNGLRCSAAKAFLKPAMNRPNLKV
                     ELGAHVERLALNGRRVVGVTYVKDGARRTAGCRIETILSAGAVMSPHLLELSGIGRGD
                     VLQRLGIPLVHELPGVGENLQDHLQLRPMYKVQGVKTLNEMDKSWIQKAWMGLEFVLF
                     RRGPLTMPPSQVGAFARSNPEVATPNVEFHVQPLSLDRFGEPLHSFPAFTTSICNLRP
                     TSRGTIHATSPDPMVAPAIDPNYLSTDEDRGVAVESLRLARRIVAAEPLRKYRPEEFL
                     PGAHLTSDDELVRGAGDCGTTIFHPVGTAKMGIASDRMAVTDERLRVRGMDNLRVIDA
                     SIMPRIVSGNTNAPSIMIGEKGAAMVIEDNRG"
     gene            20491..21531
                     /locus_tag="PLQ11_02210"
     CDS             20491..21531
                     /locus_tag="PLQ11_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HRK23745.1"
                     /translation="MPNGSGLPLGFTLALMAGTSFAIVFLLIPRLMPLFARYALARPS
                     ARSSRDTPTPQGGGAPIIAAILITLALAGLGEAFVTAAAGVKHYYWLLLAGAVVLALL
                     GALDDIQPLPAGFRLGVQTIIVALVVFGAPESWRLMPALPAPIERAVLVVAGMWFVTL
                     TNFMDGIDWMVVAEFTPMLALLLLWSAFELVSLPAGAVAATLLGGLVGFAPFNRHPAR
                     LFLGDVGSLPIGLIIAALLFEVAMRVSLAAALLLPLYFLFDATETLLRGLLGRQNVMQ
                     AHRRHAYQNAVDGGCPAPEVTAAVFQLNLGLAVLAGWAALVETAWIAGALLATGLAAT
                     ILLTRRFRIARL"
     gene            complement(21697..23682)
                     /locus_tag="PLQ11_02215"
     CDS             complement(21697..23682)
                     /locus_tag="PLQ11_02215"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRK23746.1"
                     /translation="MSKPCAIRAAFAAGLIVFSSLSQAQQSTPDQDIVLTPGRSPQAL
                     ARSGSAVSIIRSEELEKASPRSTADLLRQIPGVSVTEAGGPGQAATVRIRGSEARHTL
                     VLIDGVRANDPSTGAAEFDFNALVPGDIERIEVLRGPQSALYGSDAVGGVVNIITRKG
                     RGPTRGYASLEGGSYGSSAGKVGLSGGNQTFDYALALSGARGAGFSAYGYRIGRITAW
                     QPAGLENDAFARLGANARFGWRPVEGLSFDAGITSSINNAQYDAAFGARPDTPSYARA
                     RLTSQFLRSQFDLFGGRLRNSVTLFANQTDRSYRDISYFDFGFGLIREWDRYSYAGRR
                     LGAEYQGDLALDSYGKLIFGARIEDESLTIRTQPIETLFNTASREHARQQTRSLFALY
                     QLPIGNRLDLSFGARIDDVGKADRFATWRLTGAYRIDETGTKVRASIGTGGKAPSLFQ
                     LYSPQYGTPRLEAERSIGVDAGIDQSLFGGRLTLSATAFHNRISNLIDFSPYSLVSFT
                     YPACPVSQQFSGCYLNVGKAQTLGVELSGDAVLIEGLLTARAAYTNLVARDLATGLAL
                     ARRPGHEGRVSFRMTPLDGLTIEPSVQLVGQRFSSAGERLKLAPYARFDLAIDYRINP
                     AFSVFARGENLTGAKYQEIYNYGTTGRAAYAGIRAQW"
     regulatory      complement(23715..23935)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT         4442 a         7687 c         7563 g         4410 t
ORIGIN      
        1 cggctcgcca tcgtcattgc ctagccagac cgtcgcgaca agggcacccg tatagccgac
       61 gaaccaggcg tccttgtgtt cctgcgtggt gccggtcttg ccggccgcca gccagcctgg
      121 cagctccgcc ttgcgggcag tgccgttgac cagcgtttcg cgcatcatgc cattgagata
      181 ggccagaaca ctcggctcga tcacccggcc gcgattatgc tccttgcgct cgtagatcac
      241 cttgccgctg gcggtcctga cccggcggat gccgtagggc gcaacaccga tgccgccatt
      301 ggcgaaagcg gcataggctg tggtcagctc cagtggcgag acctcggaag tgcccagagc
      361 cagcgaggcg ttggccgcaa gcggcgcatt gatcccgagc cgctgggcgg tcctgatcac
      421 cgccttggtg ccaacctcct ggccgagctt gaccgcaatc gtattgaggg acagcgacag
      481 cgcgcgcgtc agcgacaccg ggccgaaata ctcgcgggta tagttttccg gctgccagcc
      541 cttgatgttg accggcgcgt cctcgcgcac ggtttcgggc gtcagaccgg cgtccagggc
      601 ggccagatag acaaacggct tgaacgccga acccggctga cgccttgcca gcaccgcacg
      661 gttgaactgg ctgtcggcgt aattggcgcc gccgaccagg gcgcggaccg cgccatcggg
      721 cgccatggcg acaatcgccc cctgtccgac attcttgtcc gctccctgcg catccagcgc
      781 tgccgtcaga gcccgttctg ccgcattctg taccggcatc atgaacgtgg tcgtgacctg
      841 cacgtcttcg ccgatggtgc cgacgtaatc gtcgagcatg tccatcaccc aatcggcggc
      901 ataattgacc gagccggcgc catggttgcg cctggcttcc gctggggcgg acagcgcgac
      961 cttgtgctgc tgttcgctga tatgggcctg ctcgcgcatt tccttgagca ccagcgaagc
     1021 gcgctcgcgg gccgcttccg gattccggtt gggcgcaagg cgcgagggcg atttcatcaa
     1081 ccctgccagc atcgccgatt ccgccacggt cagcagccgc gccgtcttgc cgaaatagcg
     1141 ttgggcagcc gcttcgatac cataggcacc cgaaccgaaa taaacccggt tgagataaag
     1201 ttcgaggatc tgatccttgc tgtagctgcg ctccagccac agggcgagga tggcttcctg
     1261 cgccttgcgg gcgatcgaac gctcctgggt gaggaagatg ttcttggcaa gctgctgggt
     1321 gagcgtggac cccccctgtt cgagccgcct tgccgtgaca ttgcgcatga aggcgcggcc
     1381 aagcccgaca acgtccaggc caaaatgctc gtagaaccgg cgatcctcga tcgccacgaa
     1441 ggctttggga acatacggag ggagttcgcc gatcgcaaca ttgctgccac cagtctcgcc
     1501 gcggttggcc atcagcgaac catcggccgc caggatggcg atattgggcg gccgcttcgg
     1561 cacagccagc tggtcgatcg gcggcagccg cagcgcgtaa tagccgatca atccggcaag
     1621 gccgatggca caccagaccg atgccaccgc actccagtag acaaggccgc gcagaaatcc
     1681 gcgccgccgt gttggaggac gcttctttcc gcgtggttca gccgatcgcc cggatctttc
     1741 ccgtcgcgga ggagccatgt cgaattcatc gtgatcctcg ggcggcccgc gacgagcggc
     1801 gcggcgaggg gcttcgtccc aatcatcgtc cgtatctggg gttgggcggc ggcgcggcgt
     1861 gttacggtca tgacgggatg gataggcttc gtcataggcg tcacggcgcg atcggtgatc
     1921 cgcgtcgctc cccgcacccc gccgtctgcc gcgtccgcgg tcctcgtcgt cccaatcggc
     1981 catcacacaa cccgccgccc gtgccgctcc ggtcgtcacc ttagagcgat cgccttaagg
     2041 gcccgttagc gttatcgggc cgtggcgtgg caatacggca tgatgatggc ggccaaaccg
     2101 gccaaaccgc cctgcgacgt caggtccagt caggatcagc gagaacctcg gcggtatgcg
     2161 cccccagagc cggcgaagcg cgcccggaaa cctgcggcgc cccgtcgatc aggatcggtg
     2221 aacgaaccga gggaatggtt ccggccgctg cctgatcctc caccagatcc acccgcatgc
     2281 cgcgatggat cacctgcgga tcggcaaaaa cctcgtccat cgcattgatc ggcccagccg
     2341 gaacacctgc cgcttccagc cgtgccaaca ggtcagcctt ggcaaagcga aggctgagcg
     2401 agccgatcaa cggaatgagc gcggagcgat tggcgatccg gcttgcgttg tcgatatagt
     2461 cggggtggct gccaagctca ggctggccca gcacattcat cagcttgcga tactgcccat
     2521 cattgccggt ggcgatgatg atgtggccat cggcgacggg gaacacctcg tagggcacga
     2581 gattgggatg ggcattgccc atgcggcccg gaaccttgcc cgagaccaga taattcatcg
     2641 cctgattggc gttgacggca acctgggtgt cgagcagcgc gcaatcgatg acggcgcctg
     2701 cgccggtatc cctccgccgg ttgagcgcgg cgaggatcgc cacgctggcg taaagcccgg
     2761 tgaagatgtc ggcgatcgca aagccggtct tggtcggctg gccctccgca tcgccggtga
     2821 tcgacatcgc cccgcccatg ccctggatca ggaaatcgta gccggcacgc ggcgcataag
     2881 gtccgtcctg accgaaaccg gtgatcgagc aatagatgag gcgcggattg agcgccatca
     2941 ggctctcagc atcgaggcca tacttgttca gaccaccgac cttgaaattc tcgatcacca
     3001 catcggccct ggccgccagc cggcaaatca tcgcgatgtc gtcggggttg tcgaaatcgg
     3061 cctcgatcga ccgcttgccg cgattgcagg aatgaaaata ggccgccgac agatgcccgc
     3121 catcgcgggc cggcacgaag ggcggccccc aggcgcgcgt atcatcgccg gcacccttgc
     3181 gctcgacctt gacaacatcg gcaccgagat cagccagtgt ctggccagcc catggcccgg
     3241 cgagaatgcg ggcgagttcg agaacgcgga ggccggcgag cggggcgtct gtcatgggtc
     3301 aatccagaac gtgatcgagt ccgcgcctga ggcctctgaa cccgccgacc ttgcgggcgg
     3361 ccagcagcgt acccgcgaca tagggcgcgg cagacgagcc ggcgtcgtgg cggatgacga
     3421 gccgctcgtc cggcgcaccg aagatcgcct cgcaggacag gacgaacgat ggcatccgca
     3481 ccgaatgcac ctgcaccggg atcggcccgc cgaccgcggc gccgcgcgtc gcgggaatgc
     3541 cgccgacctt gtccaccgga accgaggtgc cgtccatgcg gacctgcccg agcagttcgg
     3601 caagttcccg gcccgtgccg gagggtgaat ccggcttctt ggccgaggcg tagtcgatca
     3661 cctcgacatc cggcacatat ttcgccgcct ccagcgcaaa ccggcgcagc agcgtagcgg
     3721 tgatcgagaa attgccggcg gccagaactc cgagccttgc ggacatcgcg gcggcgtcga
     3781 tctctgcata gtcgtccgca ccgaggccgg atgtgccgat gacaacgccg cgtcccgctt
     3841 tcagggcagt gaaggtgtgg cccttcacca cctgcggctt ggtgtagtcg atgaccacat
     3901 cggagggagc ggccaatgcc tcgtccagtg tcgcgacgat ggtgacgcca ttggccgctc
     3961 cgcctgccat catcccggca tccttgccgg cagccgaacg cgaaacggcg gcaaccagcg
     4021 tcaggtccgg cgcggcatcg acagcctgca ccagcgcctt gccgacccag ccggtcgctc
     4081 cggcgatgac gacgcggacc atcagaaaaa cgcctggaga ccggtgatcg cacggccaag
     4141 gatgagggcg tgcacgtcat gggtaccctc gtaggtgttg accgtctcga ggttggcggc
     4201 gtggcgcatc acatggaact cttcggaaat gccgttgccg ccatgcatgt cacgcgcagc
     4261 ccgggcaata tcgagcgcct tgccgcaatt gttgcgcttg acgatggaaa tggcctcggg
     4321 cgccagcgtt ccggcatcaa acatgcgccc cacgcgcagc gacgcctgca gtccgaggga
     4381 aatctcggtc agcatgtcgg cgagcttctt ctgataaagc tgggtattgg cgagcgggcg
     4441 gccaaactgc cggcgatcga gaccatattg acgcgcggca tggaaacaga attcggctgc
     4501 tcccatcgcg ccccaggaaa tgccgtagcg ggcacgattg agacagccga acggcccctt
     4561 gaggccggag gcatgaggca acagcgcctc ctcgccaact tcgacattat ccagcacaat
     4621 ctcgccggtg atggaggctc gcagggataa cttgccctcg atcttcggcg ccgacaggcc
     4681 cttcatgcct ttttccagca caaaacctcg gatctcgtca ttgtgcgctt cggatttcgc
     4741 ccaaaccaca aaaacgtcgg cgatcggcga attggaaatc cacatttttg cgccgttaag
     4801 ccgataaccg ccggcggttt tgaccgcacg cgtgcgcata ccaccgggat cggagccggc
     4861 atcgggctcg gtcaggccga aacagccgat ccattcgcca ctggccagcc tgggcagata
     4921 tttcttcttc tgcgcctcgg atccgtaggc ctcgatcgga tacatcacca gcgaggattg
     4981 cacgctcatc atcgagcgat accccgaatc gacccgctcc acttcgcggg caaccaggcc
     5041 ataggagaca tagctggctc cggcgccgcc atattgctcg gccaccgtga tgccgagcag
     5101 gccgagttcg cccatctcgc ggaaaatcgc ggggtcggtc ctttcctgcg cataggctgc
     5161 gcggacgcgc ggcatcagct tgtcctgcgc ataggaacgg gccagatcac ggatcatccg
     5221 ctcgtcttcg ttcaactggt catcaagcag gaacggatcg gaccaatcga atgcggcttt
     5281 ggtgacagcg gccatggcag aaactttcag gttgcagagt ccagcacagg ccgtcttgtt
     5341 gcccgtaaaa ccattacggt gcaagggcca gaggccctac atcgttgcac cgatctgcca
     5401 gggcacgaac tcggcatcgc catagccgag ctgctcggat ttcgagcgct cgcccgatgc
     5461 aacagccaga atcctgtcaa agatctcgcg ccccttggcc tccagcgaga cgccatcgag
     5521 cacatcgccg cagttgatgt ccatgtcgtc caccatcttc tcatagatcg gcgtgttggt
     5581 ggcgagcttg atcgacggcg tcggcttgca gccgaaggcg gacccgcgcc cggtggtgaa
     5641 gcacagcaca ttcgccccgc ccgccacctg cccagtcaca ccaaccggat catatccggg
     5701 cgtatccatg aaggcaaagc ccttcacggt caccggttcg gcatagcgga acacgccgtt
     5761 catcgtggca gtgccgccct tggcggcggc gcccagcgac ttctccagaa tggtggtcag
     5821 cccgccggcc ttgttgccgg gcgaaggatt gttgttcatg tccatgcggt tgcgttcggt
     5881 ataatcctcc caccagcgga tgatctcgac cagcttctcg ccgatctcgc gggttctggc
     5941 acggcgggtg agcaggtgtt cggcgccata gatctccggg gtctcggaca gaatggtggt
     6001 gccgccatgg gcgaccagca gatccgaggc aactccgagc gccgggttgg cggtgatgcc
     6061 ggaatagcca tccgatccgc cgcattgcag cgccagcatc aggtccgagg ccgggcaggt
     6121 ctggcgccgc gcacgattgg cgatcggcag catggtgcgg atggcatcga cgccggcggc
     6181 gatggtcttt ctggtccctc ccacctcctg gatggtgaag gagcggaagg tgtcactttc
     6241 cttcagaccg taggcttcct tccagcgctg gatttggaat gcctcgcagc ccagaccgac
     6301 catcacaacc ccggccatgt tgggattggc cgcatagccc cattgcgtgc gcttgagcac
     6361 ctcatagccc tcacccttgg tcgacatcgc acaaccaagg ccatggacaa gcggaatgac
     6421 cccatcaatg ccgggatact cattcaacaa acccgaacgc atcacttcct cggccatgaa
     6481 tcgcgccacc gtggccgagc agttcaccga ggtcagaatg ccgatgtaat tgcgggtgcc
     6541 gaccttgccg ctgtcgcggc gatagccttc aaaggtggca cgccggtcca gcgcaagcac
     6601 cggctcatcc cttgcgtcgg cacaaaaggc ataatcgcga tcaaaagccg caaagtagca
     6661 attgtgctcg tgcacccagg cgccgggcag caccgcctcg gaggcaaagc cgatgatctg
     6721 gccgaacttg cggatcggct gatccttctc gatacggctg accgccatct tgtggccgcg
     6781 gggaatgcgc gccgtggcgg tcacgccgtt taccgcgctg cccaggtcaa tctggtcaac
     6841 cgcaatcacg acattgtcgg cggcattgag gcgcaggacg cgcggggcgg ggcgattggt
     6901 catggcgtaa tccggctggt gttgcggttt tggttgtcat acaagctatg gcaacccaac
     6961 ggaattcaaa aattcctgcg caacccgggc gccgcaaccg actggaacag gcacaggagc
     7021 cgaggcaccg atgaccctgt cgcttacccc tgaagccacc cttccggtcg acggaaccgc
     7081 cggcacgctg gttgcccgcg tcctggttcc cgatgtcggc ccttgcatcg cggcggtgcg
     7141 ggcggagggc gtgttcgacg tcactgcggc ctttcctacg ctgcgtgatc tgtgcgaggc
     7201 cgatgatccg gccggcgcgc tctgggcaga gaaagggctc aggatcgcaa gcctttccgc
     7261 cattcttgcc aatacgccgc caaaggacgc tgcaaagccg catctgatcg cccccatcga
     7321 ccttcaggca gtaaaggcgg cgggggtgac cttcgccgtc tcgatgctgg aacgggtgat
     7381 cgaggagcgg gcgcgtggca acccggccgc cgccgccgag atccgcaagg aagtcacccg
     7441 gctggtcggc gacgatctct cgcgcctcaa gcccggctcg cccgaggcga tgcggctgaa
     7501 gcaggtgctg atcgcacagg gggcctggag ccagtatctc gaggttggca ttggcccgga
     7561 tgcggagatc ttcaccaagg cgcagacgct gtcctcggtc ggcaccgggt tcgaggccgg
     7621 actgcatccg atgagccagt ggaacaatcc ggagccggaa gtggtgctgg tcgtctcgtc
     7681 gcgcgggcgg attgtcggcg caactctcgg caatgacgtc aatctgcgcg atgtcgaagg
     7741 ccgctcggcg ctgctgctgt cgaaggccaa ggacaacaat gcctcctgcg cggtcggccc
     7801 gttcgtgcgg ctgttcgatg cgacgttcag cctcgacgat gtgcgccaga ccgaagtgac
     7861 actgcgcgtc gagggccgcg acggattcgt tcttaatggc gcgtcgaatg tcgcaaaaat
     7921 cagccgcgac cctgccgatc tcgtcaaaca gatgatcggc cgccatcatc agtatccgga
     7981 tggcgccgtg ctgttcctcg gaacgatgtt tgcgccgacc aaggatcgcg atggcgaggg
     8041 aaagggattt acccacaagc tcggcgacat cgttaccatc gccgcgccga agctcggccg
     8101 cctgaccaat gtgatgaccc attccgacaa ggcgccggaa tggaccttcg gggtcggcgc
     8161 attgatgcgc aacctcgccc ggcgtgggct gctgtcctga cccggtcgcc gccgatcaaa
     8221 cgatgtccgg catcagaatg agatcggtat cggccgggcg ggcgccgagg ccggtgagaa
     8281 tcgcatgcgc ctgcccacga tgatgggtct ggtgattgaa aaaatgcacc accagacgcg
     8341 cattcagctg cgcaagttcg cggccggccg ctgcggaaaa ccagcgcgtt tcgcccgcca
     8401 gccaggcggg atcgaccacc tccgcccagg caaggatggc cgcatcaagc gcgtcacgat
     8461 cgtgcaccag atcgtcccag accggccgga accggggcga atccgcgatg gtcccggacg
     8521 gtttcggcga cccggaaaac cggtgcatcc agatgcagtc gccccagtag aggtggctca
     8581 gcgtgccatg cagcgagcca aaaaaggcgc cgcgatcagc ccggcgctgg gcatcagcaa
     8641 gggcacctgc cgccgcatag aggttgcggt tctgccagcg gttgtagcgc gccatcatgc
     8701 gcagatagtc gggcgtgatc atgcttgtct ccacttcacg acgttaatcc gatgccggcc
     8761 gcggcaaaaa ttgaaatcgt tgataggcgg cattcaaccc catcatgggg tcaaatgacg
     8821 tttgtctcgc gcagcggccg gccggcgacg atccgctcga aagacagatc ggcaaggtcg
     8881 agcgtccggt aggcgccggt ggcaatcagt tcagcaaggc cgcgcccgac agccggggcc
     8941 tgttgcagac cgtggccgga aaaaccggcg gcgatgtagg cgttttcgag atcgcccagc
     9001 cgtccgacga tcgcgttgtg atcgagcagg ttcatgtcat agtgcccggc ccaggcccgc
     9061 cccggccgga tcgcctcgaa ggccgggatg cgggcggcaa gattgggcca gatcacctcg
     9121 tcgaattcgt tccagttgac ctcgaagtcc tgcccctcgt cagtctcgct gtcatcggcc
     9181 ggagaatagc cgccgatgaa gccctcgcct tccggccgac accagacgcc tgacgtgtcg
     9241 atgagcaggg gaaagccatc gaggcgctcg cggcagctcc aggaaaacac gctgcgcttc
     9301 ttggcataaa ccggaatcgg cacgtcgcag gccgcggcaa gcgcacgcgc gcccgaggca
     9361 cccgccgcat tgacgaaggc gccgcaggga atgcggctgc catcatcgag gtgcaccgca
     9421 acgatgcggg gaccctgccg ctccacggca acaacacctg cctgcctgta ggtgacgcca
     9481 aggctgcgcg ccttggccgc gaatgcctgc atcagctgat agccgtcaaa ccagccttcg
     9541 ccgctgcggc cgaaactgcc gccggccagg tcctcggtcg ccagaaatgg gaacctgaca
     9601 gacaacgccg ccctgtccat cagagcaatg tcggcgccct cgcgggcctg gaggatgtgg
     9661 ttggccgaca gcaccgccat gccaggcacc gtcgccagat agagatagcc cccctccctg
     9721 agcccgatgt ccggtcttgc gccgtcaacc tccaggaggt tgccaatgtc gcgcaggaag
     9781 gcaattccgt gcatcgagat gcggatgttg acgggctgtg agaactgctg acggatggaa
     9841 cttgccgaca gcgcggacgc ggcctgccgg taactgcggt ctcgctcgac gaccaggatc
     9901 gacccggcaa agccggactg gcccgccaga tgacaagcga tggacgatcc catcaccgcc
     9961 ccgccggcca ctaccacatc gaacatcccg gcaatccctt tgatgctttc gaccagcgac
    10021 aaaataggca gcagaacagg cattgcctac ccctccctgt ttgccgtgaa cgaggtggcg
    10081 cgctaacaat caggcgacat cgcacagggg acttttaatg cctgcctcgc tcgccgacga
    10141 aacattgggc cttctcaaac gcctcggcgt tgccgaaggc cgccttgcca aatccggtct
    10201 tgccgcccgc tcgccgatca gcggcgagac gctctgtcat gtgcgcacga acagcagcgg
    10261 tgagaccaca gccgcgattg gccgggccga ggcggctttc aggctgtggc gttcggttcc
    10321 ggcgccgaag cggggcgaat tcgtgcggct gctgggtgaa gaattgcgtg ccgccaaggc
    10381 tgatctcggg cttttggtga cgatcgaggc cggcaagatc cgatccgaag gcctcggcga
    10441 agtgcaggag atgatcgata tctgcgacta tgcggtgggc ctgtcccggc aattgtgcgg
    10501 gctgacgatc gccaccgagc gcgccaacca ccgcatgatg gagacctggc atccgcttgg
    10561 cgtcgtcggc gtgatcacgg ctttcaattt tccggtggcc gtctgggcct ggaatgcggc
    10621 gctggccttc gtctgcggcg actcggtggt ctggaagccc tcggaaaaga cgccgctgac
    10681 cgcgcttgcc gtccaggcca tcgtggcgcg cgcggcggcg cgattccagg cggccggcaa
    10741 cggcatggtt ccgcaggggc tcagtgaggt gatcatcggc ggtccggaga ccggacaaac
    10801 gctggtggat gattcgcgcg tgcccctggt ctcggcgacg ggttcgacgg cgatggggcg
    10861 caaggtcggc ccgcgcctcg cccagcgatt cgcccggtcg ctgctcgaac ttggcggcaa
    10921 caatgccgcc attgtcgccc ccagcgccga tctcgacctt gccttgcgcg gcatcgcctt
    10981 tgccgcgatc ggcaccgccg gccagcgctg cacgacgctg cggcggctga tcgttcacga
    11041 aagcgtctat gaccggctga ttgcgcggct gatcaaggtc tatgacagtg tccgggtagg
    11101 cgatccgcgc gaggcaggcg tgctggtcgg cccgctgatc gatcgctccg cgctcgattc
    11161 catgcagacc gcgctcaagg acgcgcgcgc ggctggcgga cgggtccatg gcggagtgcc
    11221 agtcaggagc ctggcgggcg cctatgtgaa gcccgccctg gtggagatgc cggcgcaatg
    11281 cgggcccatg ctgcatgaga ctttcgcacc cattctctac gttgtgaaat accgcgactt
    11341 cgatgcggcc atcgccttgc agaacgatgt ggccgcaggc ctttcctcat cgctgttcac
    11401 gctcgacatg cgcgaggcgg agcggtttgt cgcggcgacc ggttccgact gcggtatcgc
    11461 caatgtcaac atcggcccgt cgggcgcgga aatcggcggc gccttcggcg gggaaaaaga
    11521 aaccggaggc ggccgcgagg cagggtcgga ttcatggaag gcctatatgc ggcgcgccac
    11581 caacacgatc aatttcggca cggatcttcc gctcgcccag ggcgtcagtt tcgatatcga
    11641 tgactgatcg ctccctaatt gatcacaact gaagcatcag ccctggttcg gctgttgcgt
    11701 gggccgatgt ggcgtgcttg gatgatcgac aaatcgtcat ccggcagtca tcgaatccgc
    11761 cgatggtggc gggaccggaa caaccccgtg acccggaacc agggagatcg ccatgtcatt
    11821 gatcagaaca gccgccttcc ttgctgcgac cgcgctgacc gccgtcgctg cgccagccat
    11881 ggcgcagacg gaaatccagt ggtggcatgc aatgaccggc gccaacaacg atgtgatcaa
    11941 caagctggcc gaggatttca acgccagcca gaaggattac aaggtcgtcg tctcctacaa
    12001 gggcagctat gccgacacga tgaacgccgg cattgcggcc tatcgggccg gcaatgcgcc
    12061 gcatatcatg caggtgtttg aagtcggcac cgcctcgatg atggcggccg gcaaggccat
    12121 caagcccgtg catgaattga tgaaagaggc cggcgaggct ttcgatccgg cgtcctacct
    12181 gccgtcggtc gccggctatt attcgacagt aaagggcgaa atgctgtcgt tcccgttcaa
    12241 ttcgtcctcg gccgtcatgt ggtacaacaa ggacgccttc gccaaggccg ggctcaatcc
    12301 ggatgcgccg cccgctacct ggccggaggt gtttgaagcc gccaagaagc ttcagggagc
    12361 cggctatgcc tcttgcggct tttcaacggc ctggatcacc tgggtcaaca tcgagcagtt
    12421 ctcgatctgg cacaacattc cgatcggcac caaggcaaat ggaatcgatg gattcgatac
    12481 cgagttcaag atcaattccc cggtccatgt gaggcactgg gccaatcttg ccgagttgca
    12541 gaagaccaag acgttcgatt attccggccg gaccaacacc ggcgaagggc gtttcactgc
    12601 cggcgaatgt ccgatcttcc tgacctcttc ggccttcctc ggcaatgtcc gtgccaacgc
    12661 caaattcgcc tgggccaatg cgccgatgcc ctattatccg gatgttgcgg gcgcaccgca
    12721 gaattcaacg ctcggcggcg catcgctgtg ggtgatgggg ggcaaggcgg ccaacgaata
    12781 caagggcgtc gccaaattct tcaccttcct gtccgacacc gaccggcagg ccaagctgca
    12841 taccgaatcc ggctatctgc ccatcaccaa ggcggcctat gagaaggtca aggcatcggg
    12901 ctattacaag gacaatccct acaacgagac gccgctcaag gaactgacca acaagccgcc
    12961 gaccgagaat tcgcgtggct tgcgcttcgg caatcttgtc cagatccgtg acatctgggc
    13021 cgaagaagtc gaagaggtgc tcaccggcaa gaaaaccccg caggctggcg ccgatgccat
    13081 ggtcagccgc ggcaatgccg tgctgcgcca gttcgaacgc accgtgtcgc gataaggatc
    13141 gggtccgccg ccccctggcg gcagccggcc atcgcgccag ggcgcaggcc ggttgaccgc
    13201 tggcggcgga ctgcatcaca tggacaagcg aaccgtcttt tccggttatg cgatcccggc
    13261 agcgctgctg ctgccgcagt tgctcattac cgtcattttc ttctatctcc ccgccggaca
    13321 ggccgtctgg cagtcgttcc agcttgagga cgcattcggc acatcaaggc agttcgtcgg
    13381 cttcgaaaat taccagatcc tgttctcaaa gccggaatat ttcaaagcca tgggcgtcac
    13441 gctggttttc tccagcgccg tagcgtttct gtcgctgtcg atttcgctgc tgctggcgac
    13501 aatggcgaac aagcacatcc gcggcgccgg cgcctacaag acgctgctga tctggcccta
    13561 tgcggttgcg ccggcggttg ccggcgtgct ctggctgttc atgttccagc cgtcgctggg
    13621 gatggttgca cagctgctca agcgctgggg cattgactgg aacccgttgc tcaatggcaa
    13681 tcacgccctg atgctgatta tcctggccgc gacctggaag cagatttcct acaatttcct
    13741 gttctttctc gccggcctgc agtcgattcc gcacagcctg atcgaggctg ccgccatcga
    13801 cggggcgcgc ccggtccggc gcttctggac cgtgatcttt ccgctgctct cgccgaccac
    13861 gttctttctt ctggtcgtca acatcaccta tgtgtttttc gacacgttcg gcatcatcga
    13921 cgccgtgacc ggaggcgggc cggccgcatc aacaacgacg ctggttttca aagtctacca
    13981 tgatggcaag tctggcggcg acctcggcct gtccgccgcg caatcggtca tcctcatggt
    14041 catcgtggtg gcattgaccg cgatccagtt ccgctacatc gagcgcaagg tgaactactg
    14101 atggtcgagc gccggtcgtt tctcaatgtc gccctgccgc acatcatcct gatgctcggc
    14161 gtcgtcatcg tgttctttcc tgtctatctc gccttcgtcg cctcgacctg ggatccggcg
    14221 acgatcatca acggaaacat gggggtgctg cccggcccgc atctgatcga gaactacaac
    14281 cggatcctgt tttccggcac ctcgtcctcg acccgcgagc cggtgatcaa catgatgttc
    14341 aattcattgt gcatggccgt gatgatcgcc gtgggcaaga tcatcatctc gatcttctcc
    14401 gcctatgcca tcgtctatta tcgatttccc ttccgcatgg ccgcgttctg gatcatcttc
    14461 gtgacgctga tgctgccggt cgaggtgcgc atctatccga cctacaagat cgtctcggat
    14521 ctcgggctgc ttgataccta tccgggcctg accatcccgc tgatcgcctc ggcgaccgga
    14581 actttgctgt ttcgccagtt cttcatgacc attcccgatg aactgctgga ggcatcgaag
    14641 atcgacgcgg cgggaccttt ccgcttcttc aaggacaccg ttctgcccct gtcgatgacg
    14701 accatcgcgg cgctgttcgt gatccagttc atctacggct ggaaccagta tctctggccg
    14761 ctcctgatca ccaccaagga ttccatggcg accatcgtca tcggcatcaa gaagatgatc
    14821 gtaacccagg acgctctcac cgaatggcag ctggcgatgg cgaccgctgt gctggcgctg
    14881 ctgccgccgg tcatcgtggt gatcagcatg cagaagctgt tcgtcaaggg attggtggag
    14941 acggagaagt gatgatgggc acggaaggat cgccacttgc cctttgcaat gggggatgcg
    15001 gccgccccgg catggttgag ccgagaccag tgccgactgc cgattgccga ttgctgaggt
    15061 cccgccatgg ctgacgtcac gctcaaagac atccgaaaag tctatcccgg cggtgtcgag
    15121 gcggtgaaag acatcgactt cgccattccc gatggcgggt tctgcgtgtt ggttggccct
    15181 tcgggttgcg ggaaatcgac cctgctgaga atgattgccg gcctcgaaac catcaccgat
    15241 ggcgaatgcc ggatcggcgg gcgcatcgtc aacgatgtcg agccggcgga tcgcgatatc
    15301 gccatggtgt tccagaacta tgcgctctac ccgcatatga cggtctacaa caacatggcc
    15361 tatggcctca ggaaccggaa gacgccggag gacgagatcg cccggcgtgt agccgaagcc
    15421 gccaggattc tggccatcga accgttcctt gaccgcaagc cccgggctct gtccggcggg
    15481 cagcgccagc gcgtcgccat gggccgggca atcgtgcgca agccgcaggt gttcctgttt
    15541 gacgaaccat tgtccaatct tgatgcgaag ctgcgggtgc atatgcgcgt cgagatcaag
    15601 aagctgcaca attcgctgaa ggtgacgtcg atctatgtca cgcatgatca ggttgaggcg
    15661 atgacgctcg ccgacatgat cgtggtgatg agcgccgggc gtatcgagca gatcggcaca
    15721 cctgccgatg tctattcacg ccccgaaagc gtctttgtcg ccacattcat cgggtccccc
    15781 gccatgaatc tggtcaaggg cgaggttgtg gctgacggcc aggtctcgct tgccggaacg
    15841 cgcctcgcct gtccgaccgg cgccgccgct gccggaacgg aggttctgat cggggtgcgc
    15901 ccggacgagg tgacgctgga ccccaccggg cccttccatt ctgcgttcac gttcatcgag
    15961 gaactcggcc cctcgcggct ctatcatgga cagatcgccg gccaggattt tgttgtgcag
    16021 gccccgcatc atgccctgtc cagcaccgtg gacagcctgc attttgccat cgatgtccga
    16081 gcggtgcatc tgttcgaggc caagagcggc aagcggatca tgacatgaag cgtttgttgt
    16141 ccgccttccg caactcgatg gctggcctgg gctgggcaat ccgccacgaa gcagcggtgc
    16201 gacaagaagc gatcctgctc gccatcggcg tgcctctggc cttcctgctg accggcgatg
    16261 gcgcgacacg cctcatgctg atcggcggcc ttgcgctgct gatggcggtg gaactgctca
    16321 acacggcgat cgaaaagctt gccgatttca tcacgcttga ccgcgatccg cgcattggcc
    16381 tggtcaagga tctcggcagc gcggcggtgt tcgtgctcat cgttctcaat ggcacgatat
    16441 gggcctgggc ggtgctgagc tggctgctcc gctaaccaga gggccaatgg catcaggcgc
    16501 ctgtctgccg gccccagggc gcaaggcgcc gatcaagatg gaccgcatcg cgcgtgaagg
    16561 acggttgcgg cggcgcatcg gtcggcttct ggccgccggc atgatcgacc agcgcggcga
    16621 tgcgcgcatc gatcggcgga tgggtggcaa acagatcgaa aaagccggca tgctcatttt
    16681 cgatgcacat ttccatgagt ccggagggag ctccgggcaa ttcagctctg cctgaaatct
    16741 tcatcagcgc cgagatcatg gcatccgggt tcttggtcag ttcaacggca ccggcatcgg
    16801 caaggtactc gcgggaacgc gacagcgcga accgcaacag gccggaggcc agccaggcaa
    16861 gaaaaatgat catcccggca atgaacaggg cggtgccggc agggctggtc ttgcgggcgc
    16921 gcggggattc gtcgggaaaa accacgcgcg gactgtaggt cgcccagcga aaagccagtt
    16981 cgccgaaaaa accgatcacc ccggcgatga tcaccgccac caccatcagc cggacgtcgc
    17041 cgttgcggat gtgggtcagt tcatgggcaa gcacggcttc gagttcgttt cggtcgagtt
    17101 ccttcaacag gccgcgcgtg accgttacgg catattgcgc ttcactcatg ccgctggcaa
    17161 aggcattgag cacatccgct tccatgatct tgagctgcgg catcgccaac ccgcgcgaga
    17221 tgcacagatt ctccaagagg ttatagagtt caggggcttc ggcgcggctg accgtatggc
    17281 tgtcggtcag tgaatcgatc atcgtctgat ggaaccggta ggcaatcgcg atccagaccg
    17341 ccgttccaat cgtggccagg ggcagtgcgg acagcgtgtt gtcgagcgac tggcggagca
    17401 tcctgtcaag tgttggcgca gggccgaagc tttcgacgac cagggcgccg gcaaacacca
    17461 ttgcatagac aagcaggaac aacccgacga tcaggatcac cgagcgtcgg cggttcgcct
    17521 ggatatggct gtaaagacca taggcgccgg ccatggcgcg ccccgtgcat cagcgctcag
    17581 aacttgacct ggggcggggt ggcatcgagg gtcttgcgcg tctcctcacc gacatcaaac
    17641 atcgacatgg ccgaaaaacc gaaccggctg gcaatcagca ccgcagggaa attctcgatg
    17701 ccggcgttga attccgcaac cgcattgttg aagaagcggc gggcggcggc gagcttgttc
    17761 tcgatatcgg acaattctcc ctgcaattgc tggaagttgg cattggcctt caggtccgga
    17821 taggcctcgg tcaaggcgat cagccgtccg agcgcgccgg aaaggccggc ttccgcctgg
    17881 gcctgcgctg ccggcccctg cgccgaaatg gcggcattgc gggcattgat caccgcctgc
    17941 aacgtctcct tttcgtgccc ggcgtaacct ttcacggtct cgaccagatt ggggatcaga
    18001 tcatggcgtt gcttgagttg gacatcgatg tcggcaaacg cctgcttggc ccgctgacgc
    18061 atgttgacga ggccgttgta gatgccgatg ccccacagga ccagcagcac cagaatacca
    18121 aggatcaccc agccagccga catgtcaccc tcccgcacgc aggaaagacc ccgcggatca
    18181 aatctgggtc aagcatagcc aaactcacgg cccgatgaaa gccaatcccg cccggccgcc
    18241 ccttgcctgc ctcatagcgg caaggccgga tcttgcagcg cgtgcagccg gtctgccgcc
    18301 agccgcccga ggcgcaccag gagttccttg cgcgtcgcag aggccagcgg aaccagcggc
    18361 gcctcccatt cgacggcggc ggaaaaacca tcgccgatga tcagaccggc atcggacggg
    18421 aagatcgcca caggcacatc cagcgctgtt gcgaagaaga atcgatcgca gtgctggcga
    18481 tagtccggcc atttggtatc ggcgcggaaa tccgccaccg aggatttgat ctcgatgatc
    18541 cagatttcac ccttcgggcc aacggccaca aggtcggccc ggcggttgga tcgcagcggc
    18601 acttcggtca gcgttgtcat gtcgaggttg cgcagaaatc gccgggtgcc gcgcgcgata
    18661 cgcaaggcag ccggcgattg ccggccgtcc tccagcgcct cggtttctgc tccgctcacc
    18721 cgcggttgtc ctcgatcacc atggctgccc ccttctcgcc gatcatgatc gacggcgcat
    18781 tggtgttgcc cgagacgatg cgcggcatga tcgaggcatc aatgaccctc aggttgtcca
    18841 tgccgcgtac ccgcaggcgt tcatcggtca ccgccatccg gtcactggcg attcccatct
    18901 tggcggtgcc gaccggatgg aaaatggtgg tgccgcaatc gcccgccccg cgcaccagtt
    18961 catcgtcgct ggtcagatgg gcgccgggca ggaactcctc cggccgatac ttgcgcaagg
    19021 gctcggccgc cacgatccgt cgggcaagac gcagcgattc gacggcaaca ccgcgatcct
    19081 cgtcggtcga cagatagttg gggtcaatgg cgggggccac catcggatcg ggggatgtcg
    19141 catggatcgt gccgcgactg gtcggcctga gattgcagat cgaggtggtg aaggccggaa
    19201 aactgtgcag cggttcgccg aaccggtcga gcgacagcgg ctggacatgg aactcgacat
    19261 tgggggtcgc cacctccgga ttggagcggg caaaggcgcc gacctgcgag ggtggcatgg
    19321 tcagcgggcc gcgccggaac agaacgaatt ccagccccat ccaggccttc tggatccatg
    19381 acttgtccat ttcattgagg gtcttgacgc cctgaacctt gtacatcgga cgcaactgga
    19441 gatgatcctg aaggttctcg ccgacacccg gcagctcatg aaccagcgga atgccaagcc
    19501 gctgcagcac atcgccgcga ccgattcccg acaattcgag cagatgcgga ctcatcaccg
    19561 cgccggcgga aagaatggtt tcgatgcggc aaccggcggt tcgtcgcgcg ccgtccttga
    19621 cataggtgac accgaccacg cgccggccat tgagcgccag cctttcgacg tgggcaccta
    19681 gttcgacctt gaggttgggc cggttcatcg caggcttcag gaacgccttg gccgctgaac
    19741 agcgcagccc gttgttctgc gtcagctgga aataggacga gccgagattg tcgccgcggt
    19801 tgaaatcatc cgttttcgga attccttctg tggctgcggc atcgcgccag gcatcgagaa
    19861 tctcccagcg ggctcgggca aaatccaccc gcagggggcc ccggtcattg tgaaaggcgt
    19921 tggccccgcc ggcatgacct tcgtggccca tgaagatggg cagaacatca tcccagcccc
    19981 agcctgcaag ccctagttgc cgccacccat catagtctgc ggcctgaccg cgcagataaa
    20041 gcatggcatt gatggctgac gagccgccga tcgccttgcc gcgcggatag gcaatgtccc
    20101 ggccattgag acccggctgc cgtgtggttc gcaaacacca gtcggaccgc ggattgccga
    20161 tggcgaacag gtagccgacc ggaatgtgga accagatcca gttgtccttg ccgccggctt
    20221 ccagcaccag cacgcgtttg ccatcggcac tcagcctgtt gccgaggatc gagccggccg
    20281 aaccggctcc gatcacgata tagtcaaagg tctcgtccgc ctgctgccca gccgtcatcg
    20341 attgtctcct gcttgccgca ctctggcaaa ggcactgcct cgacgcaacg gcggggcgag
    20401 tctgtagctt gcggcaagct gaccgatctg ccaagggaat ggctcttgac caggcggacg
    20461 gggttgtccc cgcatctttg ggaggatgca ttgccgaatg gatcaggcct gccgctcggc
    20521 tttaccctgg cactgatggc tggaacatcc ttcgcgatcg ttttcctgct gattccgcgc
    20581 ctgatgccgc tgtttgccag atatgcactg gcccgtccca gcgctcgctc cagccgtgac
    20641 acccctacac cacaaggggg cggcgcgccg atcatcgccg ccattctgat cacccttgcc
    20701 ctggccggtc tcggcgaggc ctttgtcaca gcggcagccg gggtgaagca ctattactgg
    20761 ctactgctgg ccggcgccgt tgtgctggcc ctgttggggg cgcttgacga cattcagccg
    20821 ctgccggccg gtttccggct gggtgtccag accatcattg ttgcgcttgt cgtgtttggg
    20881 gcgccggaaa gctggcgcct gatgcctgca ctgccggcgc ccatcgagcg cgcggttctg
    20941 gtggttgccg ggatgtggtt cgtcactctc accaatttca tggatggcat cgactggatg
    21001 gtcgttgccg agttcacacc gatgctggcc ctgctgctgc tgtggtcggc gttcgagctc
    21061 gtctcgctgc cggccggggc agtggccgca acattgctgg gcggtcttgt cggctttgcg
    21121 ccgttcaatc gacacccggc ccggttgttt ctgggggatg tcggcagcct gccgatcggc
    21181 ctgatcatcg cggcgctgct gttcgaggtg gcgatgcggg tgagtcttgc tgccgcactg
    21241 ctcctgccgc tctattttct gttcgatgcc accgaaaccc tgctcagggg cctgcttggc
    21301 cggcagaatg tcatgcaggc tcatcgtcgc cacgcctatc agaatgctgt ggatggcggg
    21361 tgcccggcgc cggaggtcac cgctgcggtg ttccagctca atctggggct ggcggtgctg
    21421 gcgggctggg ctgcgttggt cgaaaccgcc tggatcgccg gcgccttgct ggcgaccggt
    21481 ctggcggcaa cgatcctgtt gacgcgccgg ttccggattg cccgcctgtg accggcgctg
    21541 ttttggtaac cggagtgacc ggttttgccg gcaggatgct ggtgagcgcc ctgccggatg
    21601 cccggttgcg ccgtggttgc agcggtgcgc aaccggtctt ctgcacttcc gcccgctgtc
    21661 gaacaacggg ccatcggccc agaggttgtc gatggcctac cactgcgccc tgataccggc
    21721 ataggcggca cggccggtcg taccgtagtt atagatttcc tgatacttcg cgcctgtcag
    21781 gttctcccca cgggcgaaaa cactgaaggc cgggttgatc cggtaatcga tcgccagatc
    21841 gaagcgcgcg taaggggcca gcttgagccg ctcgccggcc gaggaaaagc gctgtccgac
    21901 cagttgcacg ctcggctcga tggtcaatcc atccagaggc gtcatccgga acgaaacgcg
    21961 gccctcatgc ccgggccgac gcgccagtgc caggccagtg gcaagatcgc gcgccaccag
    22021 gttggtatag gctgcccggg cggtcagcag gccctcgatc agcaccgcat cgcccgagag
    22081 ttcgacgccg agtgtctgcg ccttgccgac attgagatag cagccggaga actgctggct
    22141 gaccggacag gccgggtaag tgaagctgac caggctgtag ggcgagaaat cgatgagatt
    22201 ggagatacgg ttgtgaaagg cggtcgccga cagcgtcagg cgcccgccga agaggctctg
    22261 gtcgatgccg gcatcgacgc cgatcgagcg ctccgcctcc agtcgcggcg tgccatattg
    22321 cggactgtag agctggaaca gcgacggcgc cttgccgcct gtgccgatgc tggcgcggac
    22381 cttggtcccc gtctcgtcga tccggtaggc gccggtcagg cgccaggttg caaagcgatc
    22441 ggctttaccg acgtcgtcga tccgggcgcc aaacgacaga tccagccgat tgccgatcgg
    22501 cagctgatac agcgcaaaca gcgagcgggt ctgctgacgc gcatgctcgc gagaggcggt
    22561 attgaacaat gtctcgatcg gctgggtgcg gatggtcaga gactcatcct cgatccgggc
    22621 tccgaaaatc agctttccgt aggaatccag cgcaagatcg ccctgatact cggcgccaag
    22681 ccggcgtccg gcatagctgt agcgatccca ttcgcggatc aggccgaagc cgaagtcgaa
    22741 ataggaaatg tccctgtatg agcggtcggt ctgattggcg aacagcgtca ccgaattgcg
    22801 caggcggccg ccgaacaggt cgaactggct gcgcaggaac tggctggtga gccgcgccct
    22861 ggcataggac ggcgtatcgg gacgcgcgcc gaaggccgca tcatactggg cattgttgat
    22921 gctggacgta atgcccgcat cgaacgacag gccctcgaca ggtcgccagc caaagcgggc
    22981 attggcgcca agccgggcaa aggcatcgtt ctcaagccca gccggttgcc acgctgtgat
    23041 gcggccgatc cggtagccgt aggctgaaaa accggcgccg cgggcgcccg agagcgccag
    23101 cgcataatcg aaggtctggt tgccgcccga gaggccaacc ttcccggcac tggaaccata
    23161 gctgcccccc tcaaggctgg catagccacg cgttggcccg cgtcccttcc gggtgatgat
    23221 gttgacaacc ccgccgactg catccgaacc gtagagcgct gattgtggcc cgcgcagcac
    23281 ttcgatccgc tcgatatcgc ccggcaccag cgcgttgaaa tcgaattcgg cggcgcccgt
    23341 cgacgggtca ttggcccgca cgccatcgat cagaaccagc gtatggcggg cttcgctgcc
    23401 gcgaatccgg acggtggcag cctggccggg gccgccggct tcggttacag acacgccggg
    23461 tatctgacgc aacagatcgg cagtgctccg ggggctcgcc ttttcgagtt cctcggaacg
    23521 gatgatcgag acggccgagc cggatcgggc gagcgcctgc ggcgaacggc cgggggtcag
    23581 aacgatgtcc tggtccggcg tggactgctg ggcttgagaa aggctggaaa aaacaatcaa
    23641 accggccgca aaggcggcgc ggatcgcgca aggcttcgac atggtatctc ctgtccggcc
    23701 caccgccggc tctgggttaa acactgtcga ttggccggtc tcctggcttg cggagttaac
    23761 ggttgtcccc gatccttccc aggcgccttg gcccagtggc atcgcatgtg gggacaaccc
    23821 tccgcctaca gttgcggggg cagcagcgga cttggctctc agggagccgc accgcattcc
    23881 cgtttcaccc tgttgtccga acggacgtca aggcaccaac gacagccgga ccataggaga
    23941 caatcaccgg gcaaggcaag ccctgttaac ctgtccttaa ggacgaaggc ccggatcggt
    24001 tgcggatagg aagtgccccg agcgtcgggg cgatcaggag cctagggcac gccgcatttt
    24061 gatggaatca tcaaaatgcg gataacgtga tcggtatcaa ta
//
LOCUS       DAOMXG010000016        23982 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_1311_length_23982_cov_10.990680, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000016 DAOMXG010000000
VERSION     DAOMXG010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 23982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23982
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1311_length_23982_cov_10.990680"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1167)
                     /locus_tag="PLQ11_02220"
     CDS             complement(<1..1167)
                     /locus_tag="PLQ11_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRK23747.1"
                     /translation="MTLTVDVSRRLFLAGVGAAAGSFSLGMALPLPAVAQGMVPEVNA
                     WVVIHPDERVVIRVARSEMGQGTLTGLCQLVAEELEADWSKVTWEYPTPGQNVARGRV
                     WRNFSTGGSRGIRESEQYVREGGAAARMMLVAAAAMQWGVPDLECKAEKGVITHRPTG
                     RQTSFGRVAALAARIPPPPAVVLKDPATWTIAGKPLKRLDTADKLNGRQVYGADMRLP
                     GMLNAAIRDCPVFGGKLVSFDAVKVSGMPGVRAVVRVDASTVAVVADTWWQAKTALDA
                     LPIVWDEGPNAKVSSETIAAMLKEGLDADQAYEGNRRGDAKAALAGAARRIEAVYSYP
                     FQNHACMEPMNATALWTPERCEVWCPTQNGEAAFAAAVAASGLPAARCDVYKLHL"
     gene            complement(1180..1629)
                     /locus_tag="PLQ11_02225"
     CDS             complement(1180..1629)
                     /locus_tag="PLQ11_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006334234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HRK23748.1"
                     /translation="MARLHINGALREVDVTPETPLLWAIRDHIGLTGTKYGCGIAQCG
                     ACTVHVDGEAVRSCSVTVGSIRPEQKIVTIEGLSPNLSHPVQKAWLELDVPQCGYCQS
                     GMIMAAAALLARTPKPTDADIDAAMTNICRCGTYNRVRAGIKLAAGI"
     gene            complement(1743..2903)
                     /locus_tag="PLQ11_02230"
     CDS             complement(1743..2903)
                     /locus_tag="PLQ11_02230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRK23749.1"
                     /translation="MSEALNGGDRARLRVLCVTPVGLDGMGGIDRLYCYLRQHAAETG
                     APDDVVINYFASRGPASGLKSILGFPLRFLSFAWKMATGGYDIVHLNHSTDGSALRKW
                     ALGSVAKAFGVRLSTHFHGMIEESAYRTRPLWLAALRSLSAKSDRIVILGDYFSGEFR
                     DRLGIPSEKLTVIHNGIPDFSADLPLPKPVRPTRLILFAGEVGPRKGAGLLLEALAIL
                     RQRAPGWRCVMAGNGDIHEYKAMAAMLGLKDRVSFTGWVGSDEVHALMREADVVVLPS
                     RVEALPLSLIEGACAGAALVSSMAGASGDITVDGRNGHIVPLESEAIAGALAGLINDP
                     QRLAAMQLESRALFVERFRIEQFARAFRAMLLSLANRPEPVVAPTVDRRAPA"
     gene            3059..4519
                     /locus_tag="PLQ11_02235"
     CDS             3059..4519
                     /locus_tag="PLQ11_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CoF synthetase"
                     /protein_id="HRK23750.1"
                     /translation="MTQFLDVARGAYAKLPFRARAYLGSMLQFLPPRYRYGATYQAWR
                     DRLVAARKAPEIARTMQDAMRVDLVRLAYDKSPYYRAAITREFGEAFDPASVLDPAIW
                     QRLPLLSPETVSDYREALCTEPSSGLSSGSTGGTSGKPVRFFLDRNRSPIEYAFVFDA
                     WSRVGYKAGDWRAVFRGVEVSDAAGKAIEKDPALAELRFSIFNLTDDLMTRFRAEILD
                     AGIVYLHGYPSAIGMFASFLMRSGFGPMPSIRAIMLMGERLYPNYREAIEKAFPNAQL
                     VPFFGLSEKCAFAVEVPGRPDVYDFEPLYGFTELLDARNRPVTTPGQRGRIVSTGLLF
                     KGMPFLRYDTRDEATLIEAPTAANGWRLRLERIVPRRGHEFLVSRDGALIPILALVVF
                     GTEMDEIREFQFVQDTPGAALLRIVRPAGGPEPDVKPFLALMERKSGGRITLTHEFVD
                     ALPVSPRAKRRFIDQRIDVSAIENRMNLAPDAVASE"
     gene            4534..6660
                     /locus_tag="PLQ11_02240"
     CDS             4534..6660
                     /locus_tag="PLQ11_02240"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide transport family protein"
                     /protein_id="HRK23751.1"
                     /translation="MSFVIKHDGRGTAGLPVPAGNFPVPAVVQEGASPALTVDFRQLL
                     RSILRRKFWILIPLVLCSLGGLLVALTSTPKYISAVQILVDPREIQVIRSDTPLRQQI
                     PETSTTVAENALVMLRARSLLAVLIDREKLTEDSEFIGKKSPVGQADTPEARKLRALA
                     TLERRISVKRADRSSVIEAQIWTSDAGKSAKLANALADLFIEQQQDAESGTARKVATS
                     VSSKLDELAQRVQKAEREVEEYKARNSLQSANGKLVGEQQLQELNSQLVLARARASEA
                     RSRHDSIRKLSLAAIERGELPDATNSTVIGQLRLKFAEASRQEADARSRLGSKHPEMA
                     SISAQVRDARQMIADELARISRAAQGEYERAKAAEEALSRSLDQLRNQSSNTGDAMVR
                     LRELERQADANRTIYNSFLKRTRELTEQEDIATINARIVSPATPAQFPSGPGKSIVVV
                     GSTVVGGILGLLLAILAEQLDGSLRTRKQFQQVTGLPVLSEIPFGNAGRRRVGLRAPV
                     LEAPRSPAAIGAFHAADLFASRAYADRARSVLFLSFAEEAPAAEVALNISIAAAQATW
                     RVLMIDADGSGNGLSANLDLAPEWGLGDVIERRCLLSQALLTDDRTGLRILANAGAQH
                     MRTGSLRPAPQQIKASLLQPADDYELVFIDGGTIGRDATAYALAASVDDIVLVAGVGQ
                     NSSNQLRDALDMLGPFRDRVRGLITI"
     gene            complement(6760..8655)
                     /locus_tag="PLQ11_02245"
     CDS             complement(6760..8655)
                     /locus_tag="PLQ11_02245"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HRK23752.1"
                     /translation="MSPAEVLHRFHELALKRAWRSRRDGWTSFEGFDAGPIAEFPLLR
                     ARMALALPHLDAALQDTRARRYHGLGHVFDGAAHGPVWFHDPVGNTRWPGPETYCFDC
                     NVRSDGTVRGDVKFVWEINRLQMLHPLAVAIAAKADPADIDLAFAILADWQAANPPFR
                     GINWFSGIELAMRMVSLTLIVAAMPAGGWTDERRALITRMVTAYGFWLHRYPSRFSSA
                     NNHLVAEGLGLLLAGMVLPRLADAPEWRAEGRRILETEASLQIFADGVGAEQSPTYQA
                     FTMEMLAFADLVAGSAGQPLSGIVRERLIAGATFLRALMDTAGRCPAIGDDDEGRVLA
                     QPPDREPRYVASVVAMVAGCVGDPALAPPARDPHLRDALFAVPEKPETTPSGLHVFAE
                     GGYSIGRCDQGSVRSVLVFDHGPLGYLSLAAHGHADALAIWLSVGDQPVIVDAGTYLY
                     HSGGTTRNMLRSSQAHNTVTVNGASQSRPSAAFSWSSKARGQLTQVCDEEWFSVTATH
                     DGYRGRYGLIHERQIERRPNGFAVTDRLVGATRLHEAEVGFLIHPDLEVQPNGHWFRI
                     LGPAGPLVDIVGPDSLKPLIIRADASSGQGFFSPRFGELRPARRIVFAGDLGADPVVT
                     QIVIAGS"
     gene            8993..10318
                     /gene="wecC"
                     /locus_tag="PLQ11_02250"
     CDS             8993..10318
                     /gene="wecC"
                     /locus_tag="PLQ11_02250"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008126140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HRK23753.1"
                     /translation="MLMTNTIHPQASFSAQAAGRSRPAFATIAVVGLGYVGLPTAALL
                     ASRGLDVVGVDIKKDAVDRINEGRAHISEPDLDIVLNAVVKGGKLKAYTEPQPADVFL
                     IAVPTPINDDKTADMSLVKSALDSIAPVIKKGDLIVIESTSPVGTTERAAEQLRRLRP
                     DLTFPDTMPELSDIMMAYCPERILPGQTLRELIDNARIVGGLDHRSAVSARDVYRTFA
                     MGKIKLTTSRVAELSKLTENAFRDVNIAFANELSLVCDHLGVDVWSVVKMANLHPRVN
                     ILSPGPGVGGHCIPVDPWFIHEALPEATPLIRNARAVNDTKPVRVADQITRLAGRYKE
                     PVIAVLGLAYKPNVDDLRESPALRIAEDLAERNVGRLLIVEPNIEALPKGLAGKPNVT
                     LADHHTAVADADVVAILVGHSKFRNVDRAALASKVVIDTVGLLQSQDDE"
     gene            10358..12145
                     /gene="asnB"
                     /locus_tag="PLQ11_02255"
     CDS             10358..12145
                     /gene="asnB"
                     /locus_tag="PLQ11_02255"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HRK23754.1"
                     /translation="MCGFAGLVQRQQSIETSLKFRATAQRSLGRRGPDDFRAETVDGV
                     ELIHARLSIIDLANGQQPRIEPEGAIAFNGEIYNHADLHLPGVVYQTTSDTEVLLKGM
                     IHRGLDFLRRADGMFAFAYLDRRARKIYLVRDLFGIKPAYFIHKPDVFAFASTIPSLM
                     AFSDQRINPQAYLEFYLARGARGTHTLFADIHEVAPGQAVVYDLASHSIEVVAWESRR
                     QSPERNASEADLLAELDDLLHRSVQRHLVSDVPVASLLSGGIDSGLLTAIAARYVPDM
                     ACFSIGFTDKRYDESPHSKAIAEAYNLRHFVKFVDGEDLVGMLEDWPTIADDASAEPA
                     SVMRYLVARYAHDSGFKVVLSGEGADEFFGGYNQYRRFQLARQIGSVGRFFPFLGPLA
                     ERFAWPNTRLVHYLMQATRDPSYYGTSMIFEPYLIGKVFQGNIGALPKAGSLRDALFL
                     DQRYRLADDLLTITDRSTMHASIEARVPFITRTVADFATSLPESMLVRGSQQKYLLRK
                     LALKYLPHEAVERPKVGFDMPLGRWLRTDLRPMVEDALDSTWQSEFIQPGAMRRIVDA
                     HMAGKADFADKIWAFITLERNVKALKAMA"
     gene            12224..12982
                     /locus_tag="PLQ11_02260"
     CDS             12224..12982
                     /locus_tag="PLQ11_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007606847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HRK23755.1"
                     /translation="MRVSLFDTPVDILTRKETLDRAIKAMETRSRCQHVALNVAKLVN
                     ARSDRELDRDLRDSDLVGIDGMGIVFALRLFGHAVPERVAGVDLFEDLMAVCAQRGFR
                     PYLLGATQWVLDATIAELKRRHPALEFAGAQHGYFRPEDDARIAAQVRASGADCLFIA
                     MPTPRKERFMRAWRDTMNVPFLMGVGGTFDVVAGHVDRAPAIYQKLGFEWLYRLMQEP
                     RRMFWRYARTNTLFLGLILPGLVRYWLSFKRRAA"
     gene            12985..14181
                     /locus_tag="PLQ11_02265"
     CDS             12985..14181
                     /locus_tag="PLQ11_02265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRK23756.1"
                     /translation="MERGPTVLHLISSLKIGGAERLLVSMMRAAKATGGQRFVICVMN
                     RDVDDGLRAELTATGFPVVWLGRREGHLSPRYLRAIVALVDTYGIDIIHTHNDGSRTW
                     GMLAKLLRPRLKLVYTVHAEGIGRQITGLKRLAYHRMVDASVAISSFVAGECESFGAR
                     LVRTIENGVPLEAFRRTAPRTPRPSGAPLQLVNVARFAAIKGQDILIEALAMVRARGL
                     DAHLTLVGVASEPAFHAALTDQVARQGLGAHVRFVTNRTSFADILHAGDAFVLPSREE
                     GFGLVLIEAMAAGIPVIASRTGGPADLVRHGANGLLFERLDAPDLANKVIQLAADPAL
                     AARCIAGGQATAEGYDIHRTLEQHRALYDALVPAGAQTRSSLNGAQGEVTNRLSETPP
                     SFRATP"
     gene            complement(14094..15545)
                     /locus_tag="PLQ11_02270"
     CDS             complement(14094..15545)
                     /locus_tag="PLQ11_02270"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRK23757.1"
                     /translation="MMRGANFITYFLAGAVPRLGMFVILFVLAQRISIAETGLFALVV
                     TVGEMVEMTSANWLRVFVQAREAGQDRIRSLRAGRILVLALAMLVLALLTVVPIASIV
                     APSRMGQFAIAVCAYIAAFALLRLVLAVQQITHQHRLFARIELARGVTVLVTVIAATF
                     LPGASFFEPALALAGTTLIAAIWGAVAGRERVSRPAFSGHGFGTAFRFGLPVIADTGF
                     NFLIVYFDRLVLNEFLGPAGVGLYAMAFALGRQPVDFVTGPMNNLVVPVLFTARAREG
                     DARAREMQTGFAVTLTVLCAAVLVGVLLLREPIAALLVKAELRDEICNILPILTAAAC
                     VLVFKVFLYDNLFFMTGRNALKLKAVAPAALIGAMISFLMVRSHGLFGAAMAALIASC
                     LALACSVVATRSFFLFPLRYGVMAFVVGAAMIAGLALAGAAALAAPLGPLAQIMAGFI
                     AFCLVYGVALKLGGVSLKRLVTSPWAPLRDDRV"
     gene            complement(15594..16529)
                     /locus_tag="PLQ11_02275"
     CDS             complement(15594..16529)
                     /locus_tag="PLQ11_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007565977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRK23758.1"
                     /translation="MAALLTAIAVGATVITLLLPLLDRNQFKSRMKAVAIERESIRKR
                     ERERMTSENGRPVLRSAPKSYMKRMVEAFDLKKHAASEDLKAKLAAAGYRTSSAEYTF
                     LFFRLVSPIVMIGIVVIVVFVLQLVDYSPMVKVLLCILALLVGFKLPEMFLSSQTDKR
                     QVSMKRAFPDSLDLLLICVESGMSVEQSFRKVSTEIVAQSLALAEELALLTAELSYLP
                     DRRQAYENLAKRTGLESIKSVCTALVQSERYGTPVGTALRTLSQESRDQRMNEAEKKA
                     AALPPKLTVPMILFFLPVLFVVILTPAMIRVFDWK"
     gene            complement(16582..17556)
                     /locus_tag="PLQ11_02280"
     CDS             complement(16582..17556)
                     /locus_tag="PLQ11_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRK23759.1"
                     /translation="MELQQILSMVLAATAAGGLAYAVVIPYVTGDVKAEQRQKKLLVA
                     PDRSDKLGKVAPRNRQVAESLKEIERKKADQDTVTLQDRIIHAGLDWTRQKFYMVSVI
                     CGGAAGVLAFLASGFDPLFMIGGIAIGALGLPRWILIFKRNRRVKQFIQEFPNAMDVI
                     VRGVKSGLPLGDCLRIIANEAAEPVRSEFRQIVETQALGLSLAEASQGLFKRVPTPEA
                     NFFGIVIDIQSKAGGNLSEVLGNLSRVLRERRKMRDKVSAMSMEAKASAAIIGALPII
                     VAVLVGITTPDYMALLFTTEAGKFSIVISAFWMACGILSMKKMINFDI"
     gene            complement(17569..19065)
                     /locus_tag="PLQ11_02285"
     CDS             complement(17569..19065)
                     /locus_tag="PLQ11_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HRK23760.1"
                     /translation="MFGKRSTFGGGSASPAGFKPITSASTDPSPGSPAVAPQPAHASA
                     PAAPPPPVEPKRIAEAKVNTKSEEYYATKSMIFGAMIEAIDLAQLSRLDAESAREELR
                     DIVTEIISLKNVVMTIAEQEELLEDICNDVLGYGPLEPLLARDDIADIMVNGAAKTFI
                     ETGGKIQLTNIRFRDNAQLMNICQRIVSQVGRRVDEASPICDARLLDGSRVNVIAPPL
                     AIDGPALTIRKFKKDKLTLDNLVKFGSISPEGAEILKIIGRVRCNVVVSGGTGSGKTT
                     LLNCLTRYIDDDERIITCEDAAELQLQQSHVVRLETRPPNLEGNGAITMRDLVKNCLR
                     MRPERIIVGEVRGPEAFDLLQAMNTGHDGSMGTLHANSPREALSRIESMITMGGFSLP
                     TKTIREMVCSSVDVIVQATRLRDGSRRITHITEVMGMEGDVIITQDVILYQMNGEDAN
                     GKIIGKHVGTGIGRPRFWERARYYGETDRLAAALDTAGAKSKEDEQAA"
     gene            complement(19113..20384)
                     /locus_tag="PLQ11_02290"
     CDS             complement(19113..20384)
                     /locus_tag="PLQ11_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRK23761.1"
                     /translation="MMTGLDDQQASGDAHIGPLPRVSIQVFTETQEVAQVVQAACQDR
                     RMSKAHVKIQTGGAGAAVEAYRNAPTPNAIIIESRLNAQTLLSLLDELAGQCDAGTRV
                     LVIGHVNDVLLYRELMRRGVSEYMIAPLTTLGFIRAVSEVFNQDQGAGLGRIISVIGC
                     KGGVGASTVAHNIGHTIGAALDQETVIVDLDLAYGTAGLDFNQDPATGVVEAASDPER
                     LDANLLDRMLSRCQDRLSLLAAPATLERTYDFTFQNYEPILDLLRKAVPHIVIDLPHS
                     WNDWTRRVLLASDEILIVASPDLANLRNAKNMMDHLVQHRKHDRKPHLVLNQVGLPKR
                     PEIAVKEFAHAVGAEPAAVIPFDAQLFGTAANNGQMLGDVQAKSKPVEAIETLVRLVT
                     GKGGEPVKAKGALSTSVLAPILAKLRAKRAG"
     gene            complement(20408..21142)
                     /locus_tag="PLQ11_02295"
     CDS             complement(20408..21142)
                     /locus_tag="PLQ11_02295"
                     /inference="COORDINATES: protein motif:HMM:NF021025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaD family pilus assembly protein"
                     /protein_id="HRK23762.1"
                     /translation="MTSPAQKRFVLRPGAMMGRWALVVLAGLAAAGCVRRPVEVQAQI
                     PDAYQERHPIVLGDVQAKLDVFLAANGGLGYRQAGDVKDFVQQYSRQGKGALVATLPA
                     GAPGGSVRHTLGEIRKIAAANGVKPGAIQVRSGGETHPTAASVRLTFARLDARVVSKC
                     GQWPHDLAGGSTLQSWENRPYYNFGCGYQTMLAAQVADPVDMIRGRPESPIDIEKRLG
                     DLESVRENSDPTTKWPSDQTKINSAL"
     gene            complement(21202..22701)
                     /locus_tag="PLQ11_02300"
     CDS             complement(21202..22701)
                     /locus_tag="PLQ11_02300"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein family
                     protein"
                     /protein_id="HRK23763.1"
                     /translation="MARYLGSLRSAVSCTVVLVAGMALTAATPSAFAKDQGRSAMSYD
                     TVEVPKVQPGPADAHTARRFDIPLGKSIVVDLPRDAKEVFVSSPEIANAVVRTARKIY
                     IIALKPGSTSVFVNDSEGRQLAAFDIAVSKELSRELNVMREVLRTALPGAKIEVNGVG
                     ENIVLSGTVDSPLEAQRAVDIANDLVGKSRSLFFESSGSVINAMRIRGKDQVMLKVTV
                     AEVQRTVLKQLGINFDGTWKVGDSTISALMDNPLSVQKQVLSSTSIGAALNNGRQNPS
                     LKALERQGVLRTLAEPTLTAISGESAKFIAGGDIPVPQSETCDYSTGRRICTVQLTYR
                     AIGVSLNFTPVVMAEGRISIRLATEVTDLDSDNQFTGAVSNSPAFRTRKAETTVELPS
                     GGVLATAGLIMQQSRQAINGLPALMNVPILGTMFRSRDYQRSETELMVLVQPFIARPS
                     EAGQIARPDEGFADASDPSTVFMNRLVRSYGQGISRPNSFRGPVGFITD"
     gene            complement(22749..23549)
                     /gene="cpaB"
                     /locus_tag="PLQ11_02305"
     CDS             complement(22749..23549)
                     /gene="cpaB"
                     /locus_tag="PLQ11_02305"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HRK23764.1"
                     /translation="MRSSNLIVAGVALAAGLGAWVLSGPAPQPIEVQANAPAPTISTV
                     DVLVAAVEIPVGGTINDAEMKWVAFPTGASDKFITKVEGQDVMAEVRGSIARYSFAEG
                     EPLRREKIVKVSGGAGFLSAVLPVGKRAVAIKTESAGENSAGGFILPNDFVDVVAVMR
                     DEEASKARGLEVFRTTTVLTGVRVLAIGQNIQERNGEKFITGQTATLELDPVQGEKVM
                     LAQRVGTLTLALRSLRDASKSGESIEANDVKRGVNVVRFGQAQEMTVK"
BASE COUNT         4550 a         7723 c         7435 g         4274 t
ORIGIN      
        1 gagatgcagc ttgtagacat cacagcgcgc agccggcagg ccggaggccg ccaccgccgc
       61 cgcaaaggct gcctcgccgt tctgggtcgg gcaccagact tcgcagcgct ccggggtcca
      121 tagcgcggtg gcattcatcg gctccatgca ggcatggttc tggaacggat aggaatagac
      181 tgcctcgatg cgcctggccg cgccggccag cgccgctttc gcatcgccgc gccggttgcc
      241 ctcataggcc tggtcggcgt cgagcccctc cttcagcatc gcagcaatcg tttcgctgga
      301 gaccttggca ttcggcccct cgtcccagac gatcggcaag gcatcaagcg cggtcttggc
      361 ctgccaccag gtatcggcaa ccacggcgac cgtcgaggca tcaacccgca cgacggcgcg
      421 cacaccgggc atgccagaga ccttgacggc atcaaacgag accagcttgc cgccgaacac
      481 cgggcaatca cggatcgcag cattcagcat gccgggcaaa cgcatgtcgg cgccataaac
      541 ctgtcggcca ttcagcttgt cggctgtgtc gagccgtttc agcggctttc cggcgatggt
      601 ccaggttgcc ggatccttga gaacgacggc cggtggcggc gggatccgcg cggcaagagc
      661 cgcaaccctg ccgaagctcg tctgccgtcc tgtcggacga tgggtgatta cccccttctc
      721 ggccttgcac tccaaatcgg gcacgcccca ttgcatggcg gcggcggcga ccagcatcat
      781 ccgggctgcg gcgcctccct cgcgcacata ttgctcgctt tcgcgaatgc cgcgcgagcc
      841 gccagtggaa aaattgcgcc agacgcggcc gcgcgcaacg ttctggcccg gtgtcggata
      901 ttcccacgtc acctttgacc agtcggcctc gagttcctcg gcgacgagct ggcagaggcc
      961 ggtgagcgtg ccctgaccca tttcagagcg cgcaacgcgg atcaccaccc gctcgtcggg
     1021 gtgaatcaca acccaggcat tgacttcagg aaccatgccc tgtgcgactg cgggcagtgg
     1081 cagggccatg ccgagactga aactgccggc agcagcgccg acaccagcga gaaacaggcg
     1141 gcgggagaca tcgactgtca gcgtcatgat cgcctcccct cagatgccgg cggcaagctt
     1201 gatgcccgcc cgcacgcgat tgtaggtgcc gcagcgacag atattggtca tcgccgcgtc
     1261 gatatcggca tccgtcggct tgggcgtcct tgcgagcagc gctgccgccg ccatgatcat
     1321 gcccgactgg caatagccgc attgcggcac gtcgagttcc agccaggctt tctgcacggg
     1381 atgcgacagg ttcggcgaca gcccctcgat ggtgacgatc ttctgctccg gccggatcga
     1441 ccccacggtg accgaacagg agcggactgc ctcgccatcg acatgcacgg tgcaggcccc
     1501 gcattgggcg ataccgcaac cgtatttcgt gccggtcagg ccgatgtgat cacggatcgc
     1561 ccagagcagc ggcgtctccg gcgtgacatc cacctcacgc aatgcgccgt tgatatgcag
     1621 cctcgccatg cgtctgtcct ccactcagcc ttttcgggca tcgtaacgga gcgctgcgcc
     1681 aagtcatgac gcggcgcagc ggaagaccgc tgcttgcgat cacgagcgcg tgattgcagg
     1741 tctcaggctg gggcgcggcg gtcaacagtc ggagcgacaa ccggctccgg gcggttcgcc
     1801 agcgacagca gcatggcgcg gaaggcgcgg gcgaactgtt caatgcggaa gcgttcgaca
     1861 aacagcgcac gactttccag ctgcatcgcc gccaggcgct ggggatcatt gatcaatccg
     1921 gcaagcgcac cggcgatggc ttccgattcc agtggcacga tatggccgtt gcgaccgtcc
     1981 acagtgatat caccgctggc gcccgccatg gacgagacca gcgccgctcc ggcacaggcc
     2041 ccctcgatca gcgacagggg cagcgcctca acccgcgagg gcagcacgac aacatcggcc
     2101 tcgcgcatca gggcatgcac ctcatccgag ccgacccagc cggtgaagct gacacgatct
     2161 ttcaggccaa gcatggcggc catcgccttg tattcgtgga tgtcgccatt gccggccatc
     2221 acgcagcgcc agccgggcgc acgctggcgc aggatggcca gggcctccag cagcagcccg
     2281 gcgcccttgc gtggaccgac ttcgccagcg aacaggatca ggcgggtcgg ccggaccggc
     2341 ttcggcagtg gcaagtcggc cgaaaaatcc ggaatgccgt tgtggatgac ggtcagtttc
     2401 tctgaaggga tgccaagcct gtcgcgaaac tcgccggaga aataatcccc cagaatgaca
     2461 atccggtcgg atttggcgct gagcgatcgc agcgcagcca gccacagggg acgggtgcga
     2521 taggcgctct cctcgatcat gccatgaaaa tgggtcgaca ggcgcacacc gaacgccttt
     2581 gcgaccgagc cgagtgccca tttgcgcagg gcactgccat cggtggaatg gttgagatgc
     2641 acgatatcat agccgccggt tgccatcttc caggcaaaag acagaaagcg cagcggaaag
     2701 ccaaggatcg acttcagtcc gctcgccggg ccgcgcgagg caaaatagtt gatgacgaca
     2761 tcatccgggg cgccggtttc agcagcatgc tggcgcagat agcaatagag ccggtcgatt
     2821 ccacccatgc cgtccagtcc gaccggggtg acacacagca cacgcaatcg cgctcgatcg
     2881 ccgccattca gtgcctcgct catcgtgcct ccgtgcccca gatccgcgca aggttctgaa
     2941 aacctgtgcc tgatagcttt atcggatcgg acttaagaca cggtttagcg cccccggcat
     3001 ggcacatgtc ttgctgagcg cggtgtcacc cagtctgaaa ccagtaaggc gtatcgttat
     3061 gacacagttc ctggatgtag cccgcggcgc ctatgccaag ctgccgttcc gcgcgcgggc
     3121 ttaccttgga tcgatgttgc agttcctgcc gccacgctat cgctatggcg cgacctatca
     3181 ggcctggcgc gaccggctgg ttgccgcgcg caaggcgccc gagatcgccc ggaccatgca
     3241 ggatgcgatg cgagttgatc tggtccgcct cgcctatgac aagagcccct actatcgcgc
     3301 cgcgatcacc agggaattcg gcgaggcttt cgacccggcc agcgtcctcg acccggccat
     3361 ctggcagcgg ctgccgctgc tcagccccga gaccgtctcg gactatcgcg aggcgctgtg
     3421 cacagagcca agctccggac tatccagcgg ctcgaccggc ggcacgtccg gcaagccggt
     3481 gcggttcttt ctcgaccgca atcgcagccc gatcgaatat gccttcgtgt tcgatgcctg
     3541 gtcgcgggtc ggctacaagg ccggcgactg gcgcgcggtg ttccgcggcg tcgaggtgag
     3601 cgatgcagcc ggcaaggcga tcgaaaagga cccggcgctc gccgaactcc ggttttcgat
     3661 cttcaacctc accgacgacc tgatgacgcg gttccgcgcc gagatcctcg atgccggtat
     3721 cgtctacctg catggttacc cctcggccat cggcatgttc gcctcgttcc tgatgcgctc
     3781 gggcttcggc ccgatgccgt ccatccgcgc catcatgctg atgggcgaaa ggctctatcc
     3841 gaactaccgg gaggccatcg aaaaagcctt cccgaacgcg cagctcgtgc cgttcttcgg
     3901 cctgtcggag aaatgcgcct tcgccgtgga agtacccggc cgtccggatg tctacgattt
     3961 cgagccgctc tacggcttta ccgaactgct cgatgcccga aacaggcccg tcaccacccc
     4021 cggccagcgc gggcgcatcg tgtccaccgg gctgctgttc aagggcatgc cgttcctgcg
     4081 ctacgatacc cgcgacgaag cgacactgat cgaggcgccc actgcggcca acggctggcg
     4141 gttgcggctc gaacgcatcg tgccgcgccg tggtcacgaa ttcctcgttt cgcgcgatgg
     4201 cgcgctgatc ccgatcctgg ctctggtggt gttcggcacg gaaatggacg agatccgtga
     4261 attccagttc gtgcaggata cgccgggcgc ggcccttctg cgcattgtcc gcccggccgg
     4321 cggtccggag cccgatgtca agcctttcct ggcgctgatg gagcgcaagt cgggtggtcg
     4381 catcacgctt acccatgaat ttgtcgatgc actgccggtg agcccccgcg ccaagcggcg
     4441 gttcatcgac cagcgcattg acgtttcggc gatcgagaac cggatgaacc tggctcccga
     4501 tgctgttgcc agcgagtaac cggagtcgag cgcatgagtt ttgtcatcaa gcacgacgga
     4561 cgcgggacag caggtctgcc tgttccggct ggcaattttc cggttcctgc ggtggtgcag
     4621 gagggcgcaa gcccggcgct gaccgtcgat ttccggcaat tgctgcggtc cattctgagg
     4681 cgcaagttct ggatcctgat cccgctcgtt ctgtgctcgc tcggcggcct gctggttgct
     4741 ctgacgtcaa cccccaaata tatcagcgcc gtgcagatcc tggtcgatcc gcgcgaaatc
     4801 caggtgatcc gttcggatac gccgctgcgc cagcagatcc cggagacgag caccaccgtg
     4861 gccgagaacg cgctggtcat gttgcgggcc cgttcgttgc tcgcggtgct gatcgaccgg
     4921 gagaaactca ccgaagacag cgaattcatc ggcaagaagt cgccggtcgg ccaggccgat
     4981 acgccggaag cgcgcaagct gcgggcgctg gccaccctgg aacgccgcat cagcgtcaag
     5041 cgagccgacc gcagttcggt gatcgaggcc cagatctgga ccagcgatgc cgggaaatcg
     5101 gcgaagctcg ccaacgcgct cgccgacctg ttcatcgagc agcagcagga tgccgaatcc
     5161 ggaactgccc gcaaggtcgc gacctcggtc tcctcaaagc tcgacgaact ggcccagcgc
     5221 gtgcagaagg ccgagcgcga ggtcgaggaa tacaaggccc ggaacagcct gcaatcggcc
     5281 aacggcaagc tcgtcggcga acagcaattg caggaactga actcgcaact ggttctcgcc
     5341 agggcccgcg cgtcggaagc ccgttcgaga catgacagca tccgcaagct gtcgctcgcg
     5401 gccatcgagc gcggtgaact gcccgacgcc accaattcca ccgtcatcgg gcagctgcgg
     5461 ctgaaatttg ccgaagcctc gcgccaggaa gccgatgccc gctcgcgcct cgggtccaag
     5521 catcccgaga tggcctcgat ctcggcacag gttcgtgatg cccgccagat gatcgcggat
     5581 gaactggcgc ggatctcgcg cgccgctcag ggcgaatacg agcgggccaa ggccgccgag
     5641 gaagccttga gccgcagcct tgaccagttg aggaaccaat cctccaatac gggcgatgcg
     5701 atggtccgcc tgcgcgaact tgaacggcag gccgatgcca accggacgat ctataactcg
     5761 ttcctcaagc gcacccgcga attgactgag caggaagata tcgcgacgat caatgcccgc
     5821 atcgtttcgc ctgcgacccc cgcccagttt ccaagtggtc caggcaaatc catcgtggtg
     5881 gttggctcga ccgtcgtggg tggaatcctc ggcctcttgc ttgctatcct cgccgaacag
     5941 ctcgacggca gtctgcgcac gcgcaagcag ttccagcaag tcacaggact gccggtgctc
     6001 agcgaaatcc cgtttggcaa tgctggccgg cgccgcgtcg gattgcgcgc gccggtgctg
     6061 gaagcgccgc gctcaccggc cgccatcggt gcctttcatg cggcggacct gttcgcctca
     6121 cgcgcctatg ccgacagggc gcgcagcgta ctgttcctgt cgttcgcgga agaggcgccg
     6181 gccgccgaag tggcgctcaa catctcgatt gccgccgccc aggctacctg gcgtgtcctg
     6241 atgatcgatg cagacgggtc cggcaatggc ctgtcggcca acctcgatct tgcgccggaa
     6301 tgggggctcg gcgatgtcat cgagcgccgc tgcttgctca gccaggcgct gctgaccgat
     6361 gatcgcaccg ggctgcgcat cctggccaat gccggcgccc agcatatgcg cacgggttcc
     6421 ctgcgaccgg ccccgcagca gatcaaggcc agcctgctcc agccggccga cgattacgaa
     6481 ctggtgttca tcgacggggg aacgatcggc cgggatgcga ccgcctatgc gctggcggcc
     6541 agcgtcgacg atatcgtcct ggttgccgga gtcggccaga actcgtcgaa ccagttgcgc
     6601 gatgcactcg acatgctcgg accattccgc gatcgcgtgc gtggcctgat caccatctag
     6661 agcattttca agcgaagtgg atgccggttc gcgttaagaa aatgcgaaaa aacaagaaga
     6721 tagagcgctc ttacgtgaat tggaattcac taaaattgct tagctgcccg caatgacgat
     6781 ctgggtcacc acgggatcgg cgcccagatc gccggcaaag acgatccgtc gcgccggacg
     6841 caattcaccg aagcgcgggg agaaaaagcc ctgaccggat gatgcatcgg cgcggatgat
     6901 cagcggcttg agactgtccg gtccgacgat atcgaccagc ggcccggccg ggccaagaat
     6961 gcggaaccag tggccgtttg gctgcacctc aagatcggga tggatcagaa agccgacttc
     7021 agcctcatgc agccgggttg caccgacgag ccggtcggtg acggcaaagc cgttcggccg
     7081 gcgctcgatc tgccgctcgt ggatgaggcc atagcgtccg cggtaaccgt catgtgttgc
     7141 tgtaacgctg aaccattcct cgtcgcaaac ctgtgtcagt tggcccctgg ccttgctgga
     7201 ccaggaaaag gcggccgaag gcctgctttg cgatgcgcca ttcacggtca cggtgttgtg
     7261 ggcctggctt gagcgcagca tgttccgggt ggtgccgccg gaatggtaga ggtaggtgcc
     7321 ggcatcaacg atcaccggct gatcgccaac cgacagccag attgccagtg catccgcatg
     7381 gccatgggca gccaggctca gatagcccag cggaccatgg tcgaacacca ggaccgagcg
     7441 gactgagccc tgatcgcacc ggccaatgct gtagccgccc tcggcaaaga catgcaggcc
     7501 cgacggcgtc gtctcgggtt tttccggcac tgcgaacagt gcatcacgca ggtggggatc
     7561 gcgcgcgggc ggagccagcg ccggatcgcc gacgcaaccg gcaaccatcg ccacgaccga
     7621 ggcgacatag cgtggctcgc gatcgggcgg ctgggcgagc acccgcccct cgtcatcgtc
     7681 accgatggca ggacagcggc cggccgtatc catcaaggcc cgcaggaagg tggcgccggc
     7741 aatcaaccgc tcgcggacga tccccgacag aggctgaccg gcgctgccgg caaccagatc
     7801 cgcaaaggcc agcatctcca tggtgaaggc ctgataggtg ggggattgct cggcgccgac
     7861 cccgtccgca aagatctgca gcgaagcctc tgtttcgaga atgcgccgcc cttccgcccg
     7921 ccattccggc gcatcggcga gccggggcag aaccatgccc gccagcagca ggccaaggcc
     7981 ttcggcaacc agatgattgt tggccgagga aaaccgtgaa ggatagcggt ggagccagaa
     8041 gccataggct gtgaccatcc gggttatcag tgcgcgccgc tcatcggtcc agccacccgc
     8101 cggcatggcg gccacaatca gtgtcaggct gaccatgcgc atcgccaatt cgatgccgga
     8161 aaaccagttg atgccccgga acggcggatt ggccgcctgc cagtccgcca gaatggcaaa
     8221 ggcaagatcg atgtcggcgg gatcggcctt ggcggcgatg gcgactgcca gcgggtgcag
     8281 catctgcagc cggttgatct cccagacgaa cttgacatcg ccacggacag tgccgtcgga
     8341 gcggacattg caatcgaagc aataggtctc cggaccgggc caacgcgtat tgccgaccgg
     8401 atcatggaac cagaccggac catgcgccgc cccatcgaag acatggccaa ggccatggta
     8461 gcgccgggct ctcgtatcct gcagcgcggc gtcgagatgc ggcagggcca gcgccattct
     8521 tgcccgcaga agcgggaatt ccgcgatggg gccggcatcg aacccctcga aactggtcca
     8581 gccgtcgcgc ctggaccgcc aggcccgctt gagcgccagt tcatggaacc ggtgcagcac
     8641 ctccgcgggg ctcatggcgg ccagtcgggc tgcataccag cgaagccggg acatcgacat
     8701 gggcagcaca gcctccggaa cgggaacacg gttcaaactg ggacagagcc tagcccggca
     8761 agggataacg tttcataaac cgcaccggcc ggattgggca cgcggcgacg cagagggcgg
     8821 cgccggatgc gccaaaacca ccaaagctgt ccttttctgc cggtttttcg cggccggcgc
     8881 gcaagaatcg ctgaccacca ggcattccgc tcaagatctg gcactgcgct tgctgtctcg
     8941 accagccaag agcccaggcg gctcaaatcg gcacgtatcc cggagagacc ccatgttgat
     9001 gaccaacacc atccacccgc aggccagctt ctctgcccag gccgccggaa ggagccgccc
     9061 tgccttcgca acgatcgctg tggtggggct tggctatgtc ggtttgccga cggcggcgct
     9121 gctcgcctcg cgcggacttg atgttgtggg cgtcgacatc aagaaggacg ccgtcgaccg
     9181 catcaacgag gggcgtgccc atatttccga accggatctc gacatcgtgc tgaatgccgt
     9241 ggtcaagggc ggcaagctca aggcctatac cgagccgcag ccggccgatg tgttcctgat
     9301 agcggtgccg acgccgatca acgatgacaa gaccgccgac atgagcctgg tcaaatccgc
     9361 gctcgactcg attgctccgg tgatcaagaa gggcgatctc atcgtcatcg aatcgacgtc
     9421 gccagtcggc accaccgagc gcgccgccga acagctgcgc cgcctgcgcc ccgatctgac
     9481 cttccccgac acgatgccgg aactctccga catcatgatg gcctattgcc cggagcgcat
     9541 cctgcccggc cagacgctgc gtgaactcat cgacaatgcc cgcatcgtcg gcgggctcga
     9601 tcatcgatcc gccgtcagtg cccgcgacgt ctaccgcacc ttcgccatgg gcaagatcaa
     9661 gctgacgacc tcgcgtgtcg cggaactgtc caagctgacc gagaatgcct tccgcgatgt
     9721 caatatcgcc tttgccaacg aattgtcgct ggtctgcgat catcttggcg tcgacgtctg
     9781 gtcggtggtc aagatggcca acctgcatcc gcgggtgaac atcctgtcgc ccggcccagg
     9841 cgttggcggc cattgcatcc cggtcgatcc gtggttcatt catgaggcgc tgccggaagc
     9901 aacgccgctg atccgcaacg cccgcgcggt caatgatacc aagccggtcc gtgtcgccga
     9961 ccagatcacc cgcctggccg gacgttacaa ggagccggtc atcgcggtgc tcggccttgc
    10021 ctacaagcca aatgtcgatg acctgcgcga aagtccggcc ctgcggatcg ccgaggatct
    10081 ggcagagcgc aatgtcggcc gtcttctgat cgtcgagccg aatatcgaag ccctgcccaa
    10141 agggctcgcc ggcaagccga acgtgacgct ggctgatcat cacaccgctg tcgccgacgc
    10201 cgatgtggtg gccatcctcg ttggtcattc aaagttccgc aatgtcgatc gcgccgcgct
    10261 cgcttccaag gtggtgatcg acacggtggg cctgctgcag agccaggacg acgagtaagc
    10321 agaacggttt ggttgcggta ttggcaggac aagagtcatg tgtggctttg cagggctggt
    10381 tcaacgccag cagtcgatcg aaacctccct caagttccgg gcgaccgcgc agcgctcgct
    10441 cggccggcgc ggcccggacg acttccgtgc cgagaccgtc gacggggtcg aactgattca
    10501 cgcgcgtctg tcgatcatcg atctggccaa cggccagcag ccgcgcatcg agcctgaagg
    10561 ggccatcgcc ttcaacggcg agatctacaa ccacgccgat ctgcacctgc cgggcgttgt
    10621 ctaccagacc acctcggaca ccgaggtgct gctcaaggga atgatccatc gcggacttga
    10681 cttcctcagg cgcgccgatg gcatgttcgc cttcgcctat ctcgaccgga gggcgcgcaa
    10741 gatctatctg gtgcgcgatc tgttcggcat caagccggcc tatttcatcc acaagccgga
    10801 tgtgttcgcc ttcgcttcga cgatcccctc gctgatggcg ttttccgacc agcgtatcaa
    10861 cccgcaggcc tatctcgaat tctatctcgc tcgcggtgcg cgcggcacgc atacgctgtt
    10921 tgccgatatc cacgaggtcg cgccgggcca ggcggtggtc tacgatcttg ccagccacag
    10981 catcgaggtg gtcgcctggg aaagtcgccg tcaatcgccc gagcgcaacg ccagcgaagc
    11041 ggacctgctg gctgaactcg atgacctgct gcaccggtcg gtgcagcggc acctggtttc
    11101 tgacgtgccg gtggcaagtc ttttgtccgg cggcatcgat tccgggctgc tgacggccat
    11161 tgcggcccgc tacgtgccgg atatggcgtg tttttccatt ggcttcaccg acaagcgcta
    11221 cgatgaaagc ccccattcca aggcgatcgc cgaggcttac aacctgcgcc acttcgtcaa
    11281 attcgtcgat ggcgaggacc tcgtcggcat gctcgaggac tggccgacga ttgccgacga
    11341 cgccagcgcc gaaccggcgt cggtgatgcg ctatctggtg gcgcgctatg cccatgacag
    11401 cggcttcaag gtggtgctgt cgggcgaggg ggcggatgaa ttcttcggcg gctacaacca
    11461 gtatcgccgg ttccagctgg cgcggcagat cggctcggtt ggccgcttct tcccgttcct
    11521 cggcccgctg gccgagcgct ttgcctggcc gaacacccgg ctggtccact atctcatgca
    11581 ggccacacgc gatccgtcct actacggcac cagcatgatc ttcgagccgt atctgatcgg
    11641 caaggtgttt cagggcaaca tcggcgcatt gccgaaagcc gggagcctgc gcgatgccct
    11701 gttcctcgat cagcgctacc ggctcgccga cgatctgctg accatcaccg accggtcgac
    11761 gatgcatgcc tcgatcgaag cccgcgtgcc gttcattacc cgcaccgtgg cggattttgc
    11821 cacctccctg ccggaatcga tgctggtgcg cggctcgcag cagaaatacc tgctgcgcaa
    11881 gctggcgctg aaatacctgc cgcatgaagc ggtcgagcgc cccaaggtcg gcttcgacat
    11941 gccgctcggt cgctggctgc gcaccgatct cagaccgatg gtcgaggatg cgctcgacag
    12001 cacctggcag tccgagttca tccagccggg cgcgatgcgg cgtatcgtcg atgcgcatat
    12061 ggccggaaaa gccgacttcg ccgacaagat ctgggcgttc atcacgcttg agcgcaatgt
    12121 caaggcgctg aaggcgatgg cctgaggttt gctcccttcg tctgtgtaac cacgcaatcc
    12181 ggggctcatg ccccaaagcg gcacagatga aggagaaagc ccgatgcgcg tgtccctgtt
    12241 tgacacacct gtcgatatcc tgacccgcaa ggagacgctg gatcgtgcca tcaaggccat
    12301 ggaaacgcgc tcccgctgcc agcatgtggc gctgaatgtc gcaaaactgg tcaatgcccg
    12361 gtccgatcgc gaactcgacc gcgacctgcg cgacagcgat ctcgtcggca tcgacggcat
    12421 ggggattgtc tttgcgctgc gcctgttcgg acatgcggtg cccgagcgcg ttgccggcgt
    12481 tgatctgttc gaggatctga tggccgtctg cgcccaacgc ggcttcaggc cctatcttct
    12541 cggcgccacg caatgggtgc tcgatgccac catcgccgaa ctgaagcggc gccatcccgc
    12601 gctcgaattc gccggtgccc aacatggcta tttcagacca gaggacgatg cgcgcattgc
    12661 cgcgcaggtg cgggccagcg gcgccgactg cctgttcatc gccatgccga ctccacgcaa
    12721 ggagcggttc atgcgcgcct ggcgcgacac gatgaacgtg ccgttcctga tgggcgtcgg
    12781 cggcaccttc gatgtcgttg ccggacatgt cgaccgggcg ccggccatct atcagaagct
    12841 cggctttgaa tggctctatc gcctgatgca ggagccgcgc cggatgttct ggcgctatgc
    12901 gcgcaccaat acgctgtttc tcgggctgat cctgccgggg ctggtgcgct actggctgag
    12961 cttcaagcgg cgggcagcgt gaccatggag cgggggccga ccgtccttca cctgatttcc
    13021 tcgctgaaga tcggcggggc ggaacgcttg ctggtctcga tgatgcgcgc ggccaaggca
    13081 accggcgggc agcggttcgt gatctgtgtc atgaatcgcg acgtcgatga cggtctcagg
    13141 gcagagctga cggccaccgg ctttccggtg gtctggctgg gccgtcgcga ggggcatctc
    13201 tcgccccgct atctccgagc catcgtcgcg ctggtggata cctacgggat cgacatcatc
    13261 catacgcaca acgacggtag ccgcacctgg ggcatgttgg ccaagctgct gcggccacgt
    13321 ctcaagctgg tctatactgt ccatgccgaa ggaatcggcc gccagatcac cggtctcaag
    13381 cgcctcgcct atcatcgcat ggtggatgcg agcgtggcga tctcatcctt cgtcgccggc
    13441 gaatgcgaaa gcttcggagc gcggctggtc cgaaccatcg agaacggcgt gccgctggag
    13501 gcgttccggc ggacggcgcc acgaacgccg cgcccgtccg gcgctcccct gcagctggtc
    13561 aatgtggcgc ggtttgccgc gatcaagggt caggatatcc tgatcgaggc gctggccatg
    13621 gtgcgggcgc gcgggctcga cgcccatctg acgctggttg gcgtcgccag cgagcccgcg
    13681 ttccacgcgg ctctgaccga tcaggtcgcc cggcagggcc tgggcgctca tgtccgcttc
    13741 gtcaccaatc gcaccagctt tgccgatatc ctgcatgctg gcgatgcctt cgttctgcct
    13801 tcgcgcgaag aaggcttcgg actggtgctg atcgaggcca tggcggcggg aattccggtc
    13861 attgcctcac gcaccggcgg gccggcggac ctggtcaggc acggcgccaa cgggctcctg
    13921 ttcgagcgcc ttgatgcgcc tgatctggcg aacaaggtca tccagcttgc cgccgaccct
    13981 gccctggcgg cgcgctgcat tgccggcgga caggcaacgg ccgagggcta tgacatccac
    14041 cgcacgctgg agcagcatcg cgcgctgtat gacgcattgg ttccagctgg ggctcagacc
    14101 cggtcgtctc tcaacggagc ccagggagag gtgaccaatc gcttgagcga aacgccgcca
    14161 agcttcaggg caacaccata gaccaggcag aatgcgatga acccggccat gatctgggca
    14221 aggggcccca gcggtgcagc cagtgcggcc gcgccggcca gcgccaatcc ggcgatcatc
    14281 gcagcgccga ccacaaacgc catcacgcca taacgcaagg ggaagaggaa gaagctgcgt
    14341 gttgccacca cggagcaggc tagcgcgagg cagctggcaa tcaacgcggc catcgctgca
    14401 ccgaacagcc cgtgactgcg caccataaga aagctgatca tcgcgccgat caatgccgcc
    14461 ggtgccacgg ccttgagctt cagcgcgttg cgcccggtca tgaagaaaag gttgtcgtaa
    14521 aggaacacct tgaacaccag gacgcaggcc gcagcggtga ggattggcag aatattgcag
    14581 atctcgtcgc gcaattcggc cttgaccagc aacgccgcga tgggctcgcg caacagcagg
    14641 acgccgacaa ggaccgccgc acacagcacc gtcagggtca cggcaaaacc ggtctgcatc
    14701 tcacgcgccc gggcatcccc ctcgcgcgct cgcgcggtaa acagaaccgg cacaaccaga
    14761 ttgttcatcg gtccagtgac aaagtcgacc ggctggcggc cgagcgcgaa tgccatggcg
    14821 taaaggccaa cgccggcagg gccaagaaat tcgttgagca ccagccgatc aaaatagaca
    14881 atcagaaaat tgaacccggt atcggcgatg accggcagac cgaagcggaa ggcggtgcca
    14941 aatccatgcc cggagaatgc cggcctggac acacgctcgc gccccgccac tgctccccag
    15001 atggccgcaa tcaaagtggt tccggccaaa gccagcgccg gctcaaagaa gctggctccc
    15061 ggcaggaaag tggccgcgat cactgttacc aggacggtta cgccgcgtgc cagttcaatg
    15121 cgggcgaaca gacggtgctg gtgggtgatc tgctgaacgg cgagcaccag acgaagcagc
    15181 gcaaaggccg cgatataggc acagactgca atggcaaact gacccatgcg gcttggcgca
    15241 acgatagagg cgatcggcac caccgtcagc agtgcaagca ccagcatcgc cagagccagg
    15301 acaagaatcc gcccggcacg cagcgagcga atgcgatcct ggccggcctc acgcgcctga
    15361 acgaacaccc tgagccagtt ggccgaggtc atttccacca tttcaccaac ggtgacaacc
    15421 agagcgaaca gtccggtctc ggcgatggag atgcgctgcg ccagaacaaa cagaatgaca
    15481 aacatgccga ggcggggaac cgcgccggca agaaaatagg tgatgaagtt ggcgccgcgc
    15541 atcatcggcg gcccaggcgt gtggcaagca tcagcgccag cctgccacgc aggtcatttc
    15601 caatcgaaga cacggatcat cgccggcgtc aaaatcacaa cgaacagcac cggcaggaag
    15661 aacaggatca tcggaaccgt cagcttgggc ggaagcgccg ccgccttctt ttccgcctcg
    15721 ttcatgcgct ggtcgcggct ttcctgggac agggtgcgca gcgctgtgcc gaccggcgtg
    15781 ccgtagcgct cggactggac cagcgccgta cagaccgact tgatcgattc gaggccggtg
    15841 cgcttggcga ggttctcata ggcctggcga cgatccggca ggtaggacag ttcggcggtc
    15901 agcagcgcca gttcctcggc cagcgccagc gactgggcga cgatttccgt cgagaccttg
    15961 cgaaacgatt gttcgaccga catgcccgat tcgacgcaga tcagcagcag gtcgagcgaa
    16021 tcggggaagg cccgcttcat gctgacctga cgcttgtccg tctggctgga caggaacatc
    16081 tcgggcagct tgaacccgac cagcaaggcg agaatgcaca aaagcacctt caccatcggt
    16141 gaataatcga ccagctgcag gacaaagacg acaatgacga caatgccgat catcacaatc
    16201 ggcgagacca gccggaaaaa caggaaggtg tattccgccg acgacgtgcg atatccggcg
    16261 gctgccagct tggccttgag atcctccgac gccgcgtgct ttttcagatc gaaggcctcg
    16321 accatccgct tcatgtagct tttcggggcc gagcggagca ccggccggcc attctcgctg
    16381 gtcatgcgct cacgttcgcg cttgcggatg ctttctcgct cgatcgccac tgccttcatg
    16441 cggctcttga actggttgcg atccagcagc ggcagcagca gggtgatgac cgtcgcgcca
    16501 acggcaatcg ccgtcagcag cgcggccatg aagtgggcgt cgttcacata gcggatgatg
    16561 tcgctggcca cgatcgcgcc ctcagatatc gaagttgatc atcttcttca tggacaggat
    16621 gccgcatgcc atccagaacg cactgatcac gatcgagaac ttgccggctt cggtggtgaa
    16681 cagcagggcc atgtaatcgg gcgtcgtgat accaaccagc accgcgacga tgatcggcag
    16741 cgcgccgatg atcgcagccg aggctttggc ctccatgctc atggcactga ccttgtcgcg
    16801 catcttgcgc cgttcgcgca gcacgcgcga caggttgccc agaacttcgg acaggttgcc
    16861 gccggccttg gactgaatat cgatgactat gccgaagaaa ttcgcctcgg gcgtcggcac
    16921 gcgcttgaac agaccctgcg aagcctcggc cagactgagg cccagcgcct gggtctcgac
    16981 aatctggcgg aactcgctgc gaaccggttc agccgcctcg tttgcaatga tgcgcaggca
    17041 atcgccaagc ggaaggccgg atttcacgcc gcgcacgatc acatccatcg cgttggggaa
    17101 ctcctggatg aactgcttga cgcgccggtt gcgcttgaag atcaggatcc agcgtggcag
    17161 cccgagagcg ccgatagcga tcccaccgat catgaacagc gggtcaaatc cgctggcaag
    17221 gaaggcaaga acaccggcag cgccgccgca gatcacggaa accatgtaga acttctgccg
    17281 ggtccaatcg agaccggcgt ggatgatccg gtcctgcagg gtgacggtgt cctgatcggc
    17341 cttcttgcgc tcgatctcct tgagggattc ggcaacctgc cggttgcgcg gcgcaacctt
    17401 gcccagcttg tcggagcggt ccggcgcaac cagcaatttc ttctgccgct gctcggcctt
    17461 aacatcgccc gtgacgtagg gaatgacaac ggcataggcc aggccgccgg cggctgttgc
    17521 ggcgagaacc atgctgagga tctgctggag ttccatggtc gttgcgcctc aggccgcctg
    17581 ctcgtcttcc ttggacttgg caccggcggt gtcgagcgcc gccgccagac gatcggtttc
    17641 gccgtaatag cgggcccgct cccagaacct gggacggccg atgccggttc caacatgctt
    17701 gccgatgatc ttgccgttcg catcctcgcc gttcatctgg tagagaatga cgtcctgggt
    17761 gatgatcaca tcgccttcca tgcccatcac ttcggtgatg tgggtgatgc ggcgcgaacc
    17821 gtcgcgcagg cgggtcgcct ggacaatcac atcgaccgac gagcagacca tttcgcggat
    17881 ggtcttggtg ggcagcgaga agccgcccat ggtgatcatc gattcaatac ggctgagcgc
    17941 ttctcgcggg gagttggcgt gcagcgtgcc catcgagcca tcgtgaccgg tgttcatcgc
    18001 ctgcaacagg tcgaacgcct cgggaccacg aacttcgccg acgatgatcc gctcgggacg
    18061 catacgcagg cagttcttga ccagatcacg catggtgatg gcgccattgc cctcgagatt
    18121 gggggggcgc gtttcaagcc gcacgacgtg gctctgctgg agctgcaatt ccgccgcgtc
    18181 ctcgcaggtg atgatgcgct cgtcatcgtc gatgtaacgg gtcagacagt tcagaagggt
    18241 cgtcttgccg gagccggtgc cgccggaaac gacaacattg caacgcacgc gcccgataat
    18301 cttgaggatt tccgcgcctt ccggcgagat cgagccgaac ttgacgagat tgtcgagcgt
    18361 cagcttgtcc ttcttgaact tgcggatggt cagtgccggc ccgtcgatcg ccagcggcgg
    18421 ggcaatgacg ttgacgcggc tgccatcgag caggcgcgcg tcgcagatcg gcgaggcttc
    18481 gtcgacgcgg cgaccgacct ggctgacgat gcgctggcag atgttcatca gctgggcatt
    18541 gtcgcggaaa cgaatgttgg tcagctggat cttgccgccc gtttcgataa aggtcttggc
    18601 ggcgccattg accatgatgt cggcaatgtc gtcacgcgcc agcagcggtt cgagcgggcc
    18661 atagccgaga acgtcgttgc agatatcctc aagcagctct tcctgctcgg cgatcgtcat
    18721 caccacgttc ttgaggctga tgatctcggt aacgatgtcg cgcagttcct cacgcgccga
    18781 ttccgcatcc agacgcgaca gctgcgccag atcgatcgcc tcgatcatcg cgccgaagat
    18841 catgctcttg gtggcgtagt attcctcgga cttggtattg accttcgcct cggcgatgcg
    18901 cttgggctcg accggcggcg ggggagcggc aggcgccgac gcatgggctg gctggggcgc
    18961 cacggcaggc gaccccggcg acggatccgt gctcgccgac gttatcggct tgaagcctgc
    19021 cgggctcgcc gatccgccac cgaaggtgct gcgcttgcca aacatgaacc cgtgcctttc
    19081 ctacctaact cgacagtccg gactgcccgc gatcagccgg cccgcttggc gcgcagcttt
    19141 gccaggatcg gcgccaggac cgaggtcgac agcgccccct tggccttgac cggctcgccc
    19201 cccttgccgg tcaccagccg gacaagcgtt tcgatcgcct ccaccggctt gctcttggcc
    19261 tgcacgtcac ccagcatctg gccgttgttt gcggcagtgc caaacagctg ggcatcaaac
    19321 ggaatcactg cggccggctc ggcgccgacc gcatgggcaa attccttcac cgctatctcg
    19381 ggacgcttgg gcagtccgac ctgattgagc accaggtgcg gcttgcgatc atgtttccgg
    19441 tgctggacca gatgatccat catgttcttg gcattgcgca ggttggcgag atccggactg
    19501 gcaacgatca ggatttcgtc ggacgccagc agaacgcggc gagtccagtc gttccagctg
    19561 tgcggcagat cgatgacgat gtggggaacc gccttgcgca gaagatcgag tatcggctcg
    19621 taattctgaa aggtgaagtc ataggtgcgt tcgagcgtcg ccggcgcagc cagcaggctt
    19681 aaccggtcct gacagcgtga cagcatgcga tcgagcaggt tggcatccag ccgttcagga
    19741 tcgctggccg cctcgaccac gccggtggcc ggatcctgat tgaagtccag cccggccgtg
    19801 ccataggcaa gatcgagatc gacgatcacc gtttcctgat cgagcgcggc accaatggta
    19861 tgaccgatat tgtgggcgac cgtcgaagcg ccgacaccgc ccttgcagcc gatcaccgag
    19921 atgatccgcc ccaaaccggc gccctgatcc tgattgaaca cctccgagac cgcccggatg
    19981 aacccgagcg tggtcagtgg ggcgatcatg tattcggaga cgccgcgccg catcagttcg
    20041 cgatagagca gaacatcgtt gacatggccg atgaccagca cccgcgttcc ggcatcgcat
    20101 tgcccagcca gttcatcgag cagggagagc agtgtctggg cgttgagccg tgattcgatg
    20161 atgatcgcat tcggcgtcgg cgcattgcga taggcctcga ccgcagcgcc ggctccaccg
    20221 gtctggatct tgacgtgggc cttggacatg cgccggtcct ggcaggccgc ctgcaccacc
    20281 tgcgcgactt cctgggtctc ggtgaacacc tggatggaga cacgcggcag agggccgata
    20341 tgtgcgtcgc cggaagcctg ctgatcgtct aagcctgtca tcatgtcgag tgccatggtc
    20401 ccggacctca gagcgccgag ttgatcttgg tctgatccga cggccacttg gtggttggat
    20461 cagagttttc gcggacagat tcgaggtcac cgagccgctt ttcgatatcg atcgggcttt
    20521 cgggccggcc acgaatcatg tcgaccggat cagccacctg tgccgccaac atggtctgat
    20581 agccgcaacc gaaattgtaa tagggccggt tttcccagga ttgcagcgtg cttccgccgg
    20641 ccagatcatg gggccattga ccgcatttgc tgacgacccg ggcatcgagc cgggcaaagg
    20701 tgagacgcac cgaagccgct gtcggatggg tttccccgcc gctcctcacc tggatcgcgc
    20761 cgggcttgac gccgttggct gcggcgatct tgcggatttc accaagcgtg tggcggactg
    20821 agccgccggg ggcgccggcc ggcagcgtgg caaccagcgc gcccttgccc tgccgggaat
    20881 attgctggac gaaatccttc acatctccag cctgacgata gccgaggccg ccgtttgcag
    20941 cgagaaacac atcgagcttg gcctgaacgt cgcccaacac gatcgggtgg cgctcctgat
    21001 aggcatcggg aatctgggcc tggacttcaa ccgggcggcg cacgcagccg gcggcagcca
    21061 gaccggcaag gacgacaagc gcccagcgcc ccatcattgc accgggcctc aggacaaagc
    21121 gcttttgggc gggactggtc atggaaccca actccagatg tcgaaacgtg aggggacggc
    21181 ggatcatggc tgtgcgatcc gtcagtctgt gatgaagccg accggcccgc ggaagctgtt
    21241 gggccgcgaa atgccctggc cgtaggagcg caccaggcga ttcatgaata cggtcgacgg
    21301 atcagaagca tcggcaaagc cctcgtccgg gcgggcgatc tggcccgctt cggacggcct
    21361 ggcgatgaat ggctgcacca ggaccatcag ttcggtctcg ctgcgctgat agtcgcgcga
    21421 acggaacatc gttccaagga tcggcacatt catcagcgcc ggcaggccgt tgatcgcctg
    21481 acggctctgc tgcatgatca ggccggccgt ggccagcacg ccgccggatg gcaattcgac
    21541 cgtggtctcg gccttgcgcg tgcggaaggc tggcgagttc gacactgcac cggtgaattg
    21601 attgtccgaa tcgagatccg tgacctcagt ggccaaacgg atgctgatgc gcccttcggc
    21661 catgactacc ggagtaaagt tgagcgaaac accgatcgcg cggtaggtca gctgcaccgt
    21721 gcagatgcgg cggccggtcg agtagtcgca ggtttcgctc tgcggcaccg gaatgtcgcc
    21781 accggcaatg aacttggcgc tttcgccgga aatcgccgtc agggtcggct ccgccagcgt
    21841 gcgcaggacg ccttgtcgct ccagcgcctt cagcgacggg ttctgcctgc cgttgttgag
    21901 cgccgcaccg atcgaggtgg acgaaagaac ctgcttctgc accgacagcg gattgtccat
    21961 cagcgcgctg atcgtgctgt caccgacctt ccaggtgccg tcgaaattga tgccgagctg
    22021 cttgagcacg gtacgctgaa cctcagccac cgtaaccttg agcatcacct ggtccttgcc
    22081 gcggatccgc atggcattga tgaccgagcc gcttgattca aagaacaggc tacggctctt
    22141 gccgaccaga tcattggcaa tatcgaccgc tcgttgcgcc tcaagcggcg aatcaaccgt
    22201 gccgctgaga acgatattct cgccaacgcc attgacctcg atcttggccc cgggcagcgc
    22261 cgtgcgcagc acttcgcgca tcacgttgag ctcgcggctc agttccttgg acactgcaat
    22321 gtcgaaggcc gccagctggc gaccttcact gtcgttgaca aaaacactcg tcgagccagg
    22381 cttgagcgcg atgatgtaga tcttgcgtgc agtgcggacc accgcattgg cgatttcagg
    22441 gcttgaaacg aacacttcct tggcatcgcg cggcagatcg acaacgatcg acttgccgag
    22501 cggaatgtcg aagcgccgtg ccgtatgggc atcggcggga cccggctgaa ccttcggcac
    22561 ttccacggtg tcgtagctca tggcgctacg accctggtcc ttggcgaaag ctgacggcgt
    22621 agcagcggtc agcgccatgc ctgccaccag caccacggtg caggagaccg ccgaccgcag
    22681 cgagccaagg tagcgagcca tcgttgtcga tccttccgtc aaagtctcaa aaccgcgcag
    22741 ccacatgatc atttcaccgt catctcttgc gcctgtccga aacgaacgac gttgacaccg
    22801 cgcttgacat cattggcttc aatgctttcg cccgatttcg aggcgtcgcg aagactgcgc
    22861 aaggccaggg tcaacgtacc aacccgttgt gccagcatga ctttttcgcc ctgcaccgga
    22921 tcgagttcga gcgttgcggt ctggccggtg atgaatttct cgccgttgcg ctcctggatg
    22981 ttctggccga tcgccagcac ccgcacgccg gtcagcacgg tcgtcgtgcg gaacacttca
    23041 agaccgcgcg ccttgctggc ttcctcgtcg cgcatcaccg cgacaacatc gacgaaatcg
    23101 ttgggcagga tgaaaccgcc ggcactgttt tcgcccgcgg actcggtctt gatcgccaca
    23161 gcgcgtttgc caacaggcag aacggcggac aggaagccgg cgccgcccga taccttgacg
    23221 atcttttccc gtcgcagggg ctcaccctcg gcaaaggagt agcgggcgat cgagccgcgc
    23281 acttcggcca tcacatcctg cccctcaacc ttggtaatga acttgtccga ggcgccagtt
    23341 ggaaaggcca cccatttcat ctcggcgtca ttgatggtgc cgccgaccgg gatttcaacg
    23401 gctgccacaa gaacatcgac cgtggaaatc gtgggagccg gcgcattggc ctggacctcg
    23461 atcggctgcg gagccggtcc ggacaagacc cacgcaccga ggcctgcggc cagggcaaca
    23521 cctgctacga tgaggttgga gcttctcata ctgatgatcc aaatgcctga gtcacatccg
    23581 ttcggtgact tggcgatttg atcaggcaat cctcaaagta tggttaacag ttcctagcgg
    23641 cgggccagat tgtcgcccta attcgggaca gggtaaggac ggatcggcca atatacccgg
    23701 tggacttgcg tcatgatcga ttgcgtcact cgctaatcaa cgctcgtttc atgccagaat
    23761 tgactgcgca gcgatgcgcc aatatccagc cggtcattcg acaatgatca acgacagcct
    23821 gccccggcac cactgatgta atgacaaagc cagtgtccaa agcagcgctt tataccaaaa
    23881 gtcattgata atgactcttt ggtgcacctc aaaaatttct catctctttg ataaaagtga
    23941 gaaatttttg aaacgaacca agtcgcggcg agttcctcgc ct
//
LOCUS       DAOMXG010000017        23162 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_1365_length_23162_cov_9.176440, whole genome shotgun sequence.
ACCESSION   DAOMXG010000017 DAOMXG010000000
VERSION     DAOMXG010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 23162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23162
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1365_length_23162_cov_9.176440"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="PLQ11_02310"
     CDS             <1..754
                     /locus_tag="PLQ11_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRK23765.1"
                     /translation="IGQRGGLAVGPATPLILRGKAPVLGWAPPMLPRAGDDLAVRLLD
                     LYQHRDPALMKSLAAALETDKMARANGMNVGGGANPQRMVQVAEGVAKLMAAADGPRV
                     AALAYEGWDTHANEGGAKGRLATLLAGLDGSFQAFERLLEPVWKDTAVLVVTEFGRTA
                     RINGTEGTDHGTATVAFLLGGAVKGGRVLADWPGLRPAALFEGRDLRPTTDLRAVLKG
                     VLADHLGLGANVLAGDVFPDSGAVKPLTGLIA"
     gene            796..1149
                     /locus_tag="PLQ11_02315"
     CDS             796..1149
                     /locus_tag="PLQ11_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004688588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HRK23766.1"
                     /translation="MLNLTSPALLPILMLIASNVFMTVAWYGHLKFKTSPLWVAVMAS
                     WGIAFFEYWLAVPANRIGHQVYSAAELKTMQEVITLVVFAGFSVFVLGEMLTWRHALG
                     FALIAAGAAIIFHGR"
     gene            1212..1994
                     /locus_tag="PLQ11_02320"
     CDS             1212..1994
                     /locus_tag="PLQ11_02320"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRK23767.1"
                     /translation="MNVSASPRIAISSGIPALDVYLERDYETVKGMSSRFAAAIAGHL
                     IAFQARRGIVGDIAEIGAFKGRFFIAMMLAAEAGDRGLGIDVFSWPSDATYDEFVANC
                     AAHGLKAPRIDAWKADTRTMGVRDLRKRLLGRAVRFFHIDGDHSDICLTKDLELATKV
                     MHPEGILCLDDMLHPGYPTLVATVMRYLKRHPEMGVFCVVDREDIVAAPKFMLCRRTM
                     KDTYEAELMRAFPQFHWVLGADFGAEDWCVVLTPSPRLAAVD"
     gene            complement(2002..2127)
                     /locus_tag="PLQ11_02325"
     CDS             complement(2002..2127)
                     /locus_tag="PLQ11_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23768.1"
                     /translation="MLKDSLKDDLPLSIIVSSLIIGIIFAAATLYLNGLGTLLIR"
     gene            2407..4176
                     /locus_tag="PLQ11_02330"
     CDS             2407..4176
                     /locus_tag="PLQ11_02330"
                     /inference="COORDINATES: protein motif:HMM:NF017775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HRK23769.1"
                     /translation="MDVKPDKAGPGGGAPVQATVWHDLGWLFAALRQAPTRGRIALIA
                     AAIVVILIANMVAQVQLNRWNGQFFDALSAKNFDLFLTMTGVFFMLMAVLLSLVVAQT
                     LAQELLKIELRQFLTTHLADLWLARARPYRLVFAGDIGANPDQRVQEDTRKLAELAAE
                     LVVGMTQQIFLLTTFIGVLWTLSSELKFWIGGQEIYIPGYMVWCALIYAGLGSLFAFL
                     VGRPLVRLNAERSQKEAELRFALVRLTESAESIGFLNGEADERRNLNQPIGAVIGILR
                     QIAFKLARLTWVTSGYGWLSLIVPVLVAAPGYFSGAVTFGGLMRLIDAFTQVQASLRW
                     LVDNVAKIADCRAALYRVMRFEEELRTSHLEEAELKGIHVEAGGAGRLELEGVRVTMP
                     NGRPVIAAASFRLAPGERVQLEGLAGTGKSTLLRAISGLWPWGGGTIRRPDAEGLMFL
                     PPRPYLPLGSLREAVCYPDPPERFSEAAVQSAMQRCGLGGRIDRLDHVRRWDRDLTIG
                     EQQRLTFARLILHRPALVIMDEATSALDEPGQAGLIRLFDEELKDSAVLFVSHRGGYG
                     GLAERRMRLEPGAEGSVLVDSVR"
     gene            4257..4586
                     /locus_tag="PLQ11_02335"
     CDS             4257..4586
                     /locus_tag="PLQ11_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009449906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRK23770.1"
                     /translation="MLRILGVLIALAAAGAAEAQDSGRDIRLGKRIAEKNCANCHSIG
                     LKGASTRKGAPPFRTLHRRYDLDNLQEALVEGVAVGHRGADMPEFTFTPDQTDALIAY
                     LKSLKGR"
     gene            4739..5440
                     /locus_tag="PLQ11_02340"
     CDS             4739..5440
                     /locus_tag="PLQ11_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018260527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRK23771.1"
                     /translation="MTSWVDFWNRDPSIYVNERHKMLHYRQVARDIAGLIDNGQARVL
                     DHGSGEATSADLVAARCAELVLCDAAPNTRARIAAQFAGKDTIRAMAPEDLPSLPDGH
                     FDLIVANSVLQYLDEVTLEASLEEWKRLLKPDGRLILADVIPPNVSPLADARALLGFA
                     MTGGFLVAAIGGLIRTALSDYRKLRSELGFSTHSEADLVARLQRHGFAVRRLDHNIGH
                     NPARMSFEARPVSES"
     gene            complement(5429..5797)
                     /locus_tag="PLQ11_02345"
     CDS             complement(5429..5797)
                     /locus_tag="PLQ11_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012707579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HRK23772.1"
                     /translation="MTQLLWLALGWCAVGLGIAGAVLPILPTTPFMLVAAYCFARSSP
                     RLHRWIVEHPTFGPPVRHWQAHGAIARRTKWLACGSMAAVLAISLLLQLKPLVILIQA
                     VCLAGAATFIVTRPEPPSGL"
     gene            complement(5794..6180)
                     /locus_tag="PLQ11_02350"
     CDS             complement(5794..6180)
                     /locus_tag="PLQ11_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HRK23773.1"
                     /translation="MFALLRQFVAFFGVGLVAAIVHYGVLIAGVEGFGLRAVPATLAG
                     YILGGIVSYLLNRRHTYRSERPHEEAGWRFAVVAGVGFVLTYGLMSLFVERMSLPYLP
                     AQIVTTGLVLVWSFFAHKYFSFGDRA"
     gene            6671..10024
                     /gene="carB"
                     /locus_tag="PLQ11_02355"
     CDS             6671..10024
                     /gene="carB"
                     /locus_tag="PLQ11_02355"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HRK23774.1"
                     /translation="MPKRTDLHTILIIGAGPIVIGQACEFDYSGTQAVKALKAEGYRI
                     VLVNSNPATIMTDPDMADATYVEPITAEIVAKIIEKERGDRSKGFAVLPTMGGQTALN
                     CALSLNRKTLDDGKGGKTSVLEKFGVELIGATAHAIDKAEDRELFREAMTKIGLETPR
                     SHHVKTLGAALEALKDIGLPAIIRPSFTMGGTGGGIAYNKEEFIDIVERGIDASPTSE
                     VLIEESVLGWKEYEMEVVRDKNDNCIIVCSIENIDPMGVHTGDSITVAPALTLTDKEY
                     QRMRDASLAVLREIGVETGGSNVQFAVNPADGRMIVIEMNPRVSRSSALASKATGFPI
                     AKVAARLAVGYTLDEIANDITGGATPASFEPTIDYVVTKIPRFAFEKFPGADPVLTTS
                     MKSVGEAMAIGRTFHESLQKALRSLETGLTGLDDIEIEGLGRGEDHNAIRKAIGTPTP
                     DRLLQVAQAMRVGFSLEDIHASCRIDPWFLERLKEIVDMEAMVRAHGLPTTPGAFRQI
                     KAMGFSDKRLATLVKSTEVEVRAARRALGVRPVYKRIDTCAAEFASPTAYMYSTYEAP
                     FACAPADEARPSNARKAVILGGGPNRIGQGIEFDYCCCHACFALAEAGYETIMVNCNP
                     ETVSTDYDTSDRLYFEPLTEEDVLEIMAKEMEAGELAGVIVQFGGQTPLKLAEALEEN
                     DIPILGTSPDAIDLAEDRDRFKRLLDKLKLRQPQNGIAYSVEKARDEAEKLGFPFVVR
                     PSYVLGGRAMAIIRDREAFDDYLLGTLPSLIPSDIKARYPNDKTGQINTVLGRNPLLF
                     DRYLTDAIEVDVDCLADGKDAFICGIMEHIEEAGIHSGDSACSLPARSLSAETIAELE
                     RQTRALALALDVVGLMNVQYAIKDGVIYVLEVNPRASRTVPFVAKVVGTPFAKIAARV
                     MAGEKLRSFDLKSEPYDHVGVKEAVFPFNRFPGVDTVLGPEMRSTGEVMGLDRSFGVA
                     FAKSQLGAGSKVPKSGTVFVSVRDDDKPRLLESIRELVRLGFRIIATGGTQRYLVEQG
                     VKCERINKVLEGRPHIVDEIKNGRVQLVFNTTDGAQAMADSKELRRQALLAKVPYYTT
                     LAGAVAAAEGIAAYLGGDLEVRALQDYAR"
     gene            10133..10606
                     /gene="greA"
                     /locus_tag="PLQ11_02360"
     CDS             10133..10606
                     /gene="greA"
                     /locus_tag="PLQ11_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HRK23775.1"
                     /translation="MERVPLTPQGFAALEAELKRRQQEERPRIILAIQEARAHGDLSE
                     NAEYSSAKEQQALNEGRITELESLISRADVIDISKLSGSKVKFGATVTLVDEETEKER
                     AYMIVGETEADVKLGRVSISSPIARALIGKSVGDSVEVNTPGGAKSYEIVKVAFR"
     gene            10718..12409
                     /locus_tag="PLQ11_02365"
     CDS             10718..12409
                     /locus_tag="PLQ11_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRK23776.1"
                     /translation="MSAKTALDRPWLRHYPAGMPHEIDVAAGGTLADILRDCAKRYAD
                     KPAFESFEKKLSFAEMFAMAEAVASWLHQQGCVKGERIGLMMPNVMAYPASIFGVILS
                     GCTVVNINPLYTVRELNHQLKDSGATTLFVLENFASVAQDGIKGTNVKRVVIVKPGDL
                     LGLKGKIINLVSKYVKKAVPAWSIPGTIAFSDVIAAGRSKPMNAVTITQDDVAFLQYT
                     GGTTGVSKGATLLHRNVVANVLQCDMWLSPKMQGEPNHVMVTALPLYHIFGLTCCCVM
                     MWRMGAMCLLIANPRDFAGFIAIIKKCRVTLFSGVNTLYNALANHPDIKGVNFSSLKI
                     SVSGGMAVQSAVARKWKELTGTAILEGYGLSETSPVVTLNDPFLEEFSGTIGFPVAST
                     DVAIRDEAGQDLPAGQSGELCVRGPQVMAGYWQRPDETAKTMTADGFFRTGDIAMMLP
                     DGQFKIVDRLKDMILVSGFNVYPNEVEDVLASHPGVLEVAVIGVPDAGSGEAVAAYVV
                     KKDPELTADAVRAFAKEKLTAYKVPKQVHFRDSLPKTNVGKLLRRVLREEVLGKS"
     gene            12526..13479
                     /gene="msrP"
                     /locus_tag="PLQ11_02370"
     CDS             12526..13479
                     /gene="msrP"
                     /locus_tag="PLQ11_02370"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007567812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HRK23777.1"
                     /translation="MLIRRPHSWELPESAVTPDGVAPSRRQMLGGGLALLASASAAGA
                     QRVDDDPSMVLYPFARNDAYPPRGKVTDETLVANYNNFIEFGSGKRIQRAAQALVVRP
                     WTIRIDGQVEKPIEMGIDDLLRLLPREERIYRHRCVETWAKVVPWSGVPLKALLDHVR
                     PLASAKYVRFETFHDPKMAPGQRSPVYGFPYSEAITIAEAANELAFLATGAYGKPLKK
                     QFGAPVRLVLPWKYGFKSAKSVVRISFVEKRPVSLWEKLQPDEYGFWANVNPDVPHVR
                     WSQEEEEVLGSYSRTKTLIFNGYAEQVAALYRGLENEKLFT"
     gene            complement(13951..14931)
                     /locus_tag="PLQ11_02375"
     CDS             complement(13951..14931)
                     /locus_tag="PLQ11_02375"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRK23778.1"
                     /translation="MSTHLNRRSWLAAAGAALALPQMASAQGFPNRVVRIVVPYPPGG
                     PTDALARIIANEIQGDLGQTVIVENKPGGAGSIGTRGVARGETDGHTIVLGNNQTHVN
                     NAFLVKEAGYDPIKDFAPLAGLADLQHVLVVTNALAATKVSELVALAKKDPGKFNYGS
                     TGIGSGSHLAMELFKTRTGTDMTHIPYQGAGPMAAEVMAGRIELAFATLPSVLGQIRG
                     GSMRALAVASLNRAPQLPDVPTLKEQGLVDSEADSWLALFSPAATPPEARDRLTKAVL
                     GALGKPAVAEGVVKLGFALSLREPAAFATYHKAEMLKWEAVIKAAKVEPQ"
     gene            15057..16259
                     /locus_tag="PLQ11_02380"
     CDS             15057..16259
                     /locus_tag="PLQ11_02380"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRK23779.1"
                     /translation="MAFLADILSVVKPSATIVLTQMARELKAKGKDVISLSVGEPDFD
                     TPQNIKDAAIAAIQRGETKYTPVAGIQPLREAIAAKFKRENGLTYKPSQIIVGTGGKQ
                     ILFNAFMATLNKGDEVIIPAPYWVSYPEMVALCGGTPVSVLTRPENKYKLQADELEKA
                     ITPRTKWLVFNSPSNPTGGAYTRAELRALTDVLIKHPHVWVLTDDMYEHLTFGDFVFT
                     TPAQVEPALYDRTLTMNGVSKAYAMTGWRIGYAGGPEALIKAMDMVQGQQTSGACSIA
                     QWAAVEALNGPQDFIPMSKKVFEQRRDLVVSMLNQCRGITCATPEGAFYVYPSCRDLI
                     GLKAPSGKVIATDEDFVMELLNEEGVATVHGSSFGLGPNFRVSYATSNDKLEEACRRI
                     QRFCNALT"
     gene            16678..17382
                     /locus_tag="PLQ11_02385"
     CDS             16678..17382
                     /locus_tag="PLQ11_02385"
                     /inference="COORDINATES: protein motif:HMM:NF019896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase family
                     metallohydrolase"
                     /protein_id="HRK23780.1"
                     /translation="MRAAAAKEDAAKARLSGAASAPAAKAEPEPPARATGIFALLSGT
                     GAPSVDAPANDAAEQSILVRAVGAAPDRPVEGPSSRATQVTRLDLSNPFLPKPMSPSD
                     EEGLDDEEASKIAEKQVDSVQIACLKPSLMAMIQKAGEHFGAKPVITSGFRPYGRRGS
                     YHRRCEAADFFIPDVPAGRLVQFLRNLPGAGGVGTYCHTKSVHLDTGEPRNWHQCGFR
                     RSFALRVPALAENGSR"
     gene            17430..17657
                     /locus_tag="PLQ11_02390"
                     /pseudo
     CDS             17430..17657
                     /locus_tag="PLQ11_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019906726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ada metal-binding domain-containing protein"
     gene            17732..18745
                     /locus_tag="PLQ11_02395"
     CDS             17732..18745
                     /locus_tag="PLQ11_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007567782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mitochondrial fission ELM1 family protein"
                     /protein_id="HRK23781.1"
                     /translation="MPPSSVPDLALARAGVAGARVWVLSDGKAGDEVQCLGVAERLGL
                     EPELRRVKPRAPYLWLMPWGGIDPAEAPGRPGSPLAGPLPDIAIASGRRTASYLRKVK
                     ALSGGRCFTVFLKDPRCGAGTADFLWVPEHDRLRGANVLATLTSPHRISPERLAAART
                     NPPYGLDRLPGPRVAVLIGGDSAHHRFTPGDIATLLAHCTRLAETGATLMATASRRTP
                     PALAGALTELVRGSGGFFWDGTGENPYIALLALADAVVVTADSVNMAGEAAATGKPVL
                     LFEPSGGSDKITRFLRQLERAGVVHRFDGTIAGSPYQPIDSTPAIAAELARRYLLHRR
                     ALS"
     gene            18758..19711
                     /gene="trxB"
                     /locus_tag="PLQ11_02400"
     CDS             18758..19711
                     /gene="trxB"
                     /locus_tag="PLQ11_02400"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HRK23782.1"
                     /translation="MSTAPSHTKVLIIGSGPAGYTAAIYAARALLKPVMISGFEPGGQ
                     LMITTDVENYPGFADVIQGPWLMEQMRLQAEHVGTEMISDHVTKVELGQRPFAVTCDS
                     GARFTADALIIATGAKAKWLGLPSEESFKGYGVSACATCDGFFYRGKDVVVVGGGNSA
                     VEEALYLANLASSVTLVHRRDTLKAEKILQDRLFKHPKLRFLWDSAVEEICGEKDPPG
                     VTHLRIRNVKTGALSDHRTHGLFVAIGHAPASELFAGQVTLKPSGYIATVPGSTATNI
                     PGVFAAGDVADETYRQAVTAAGMGCMAALEAERWLHAQAGA"
     gene            19864..20754
                     /locus_tag="PLQ11_02405"
     CDS             19864..20754
                     /locus_tag="PLQ11_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRK23783.1"
                     /translation="MDWDKVRIFHAAAAAGSLTKAGDVLGLSQSAVSRQVSALEGELK
                     VPLFHRHARGLVLTEQGELLFRAARDLVQKLEQTQAQLMDSRQKPDGLLKVTANVGLG
                     AHWLTPRLEEFIEQYPDIRLEIILTDDELDLAMREADVALRLREPIQGDLIRRKLFTV
                     HFHAFASPDYLKRYGQPRLPVDLDQHRLLAFGGDIPSYLKNLNWHLTAGRDASGPRVP
                     ALTVNSLTSLMRGAQRNAGIAVLPDYLVEPNTGLVQVLASESMPQLECFLVFAEALKG
                     GARVQAFRDFIVAKAQRWAY"
     gene            complement(21136..22320)
                     /gene="carA"
                     /locus_tag="PLQ11_02410"
     CDS             complement(21136..22320)
                     /gene="carA"
                     /locus_tag="PLQ11_02410"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HRK23784.1"
                     /translation="MSTATGWNEPKPTAVLVLADGTVIEGHGAGAIGSATGEVCFNTA
                     MTGYQEILTDPSYTGQIITFTFPHIGNVGVNDEDVETVNMASESGVRGAVLQSPITNP
                     SNYRSARHFDAWLKARNIIAITGVDTRALTALIRDKGMPNAVIAHDPSGNFNLAALKA
                     AARAVPSIDGQDLVPAVATTQRFGWDETDWLWGRGYGRQDKPSFNVIAIDYGVKRNIL
                     RLLADRGCKVTVVPPTMPAADILAMRPDGIFLANGPGDPAATGEYSVPTIKALLDAKV
                     PTFGICLGHQMLALALGAKTLKMPQGHHGANHPVKEHATGKVEIVSMNHGFAVDPATL
                     PAHAEETHVSLFDGSNCGLKLKDRPAFSVQHHPEASPGPRDSHYLFDRFVTLMQAAKK
                     AA"
     gene            22477..22929
                     /locus_tag="PLQ11_02415"
     CDS             22477..22929
                     /locus_tag="PLQ11_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HRK23785.1"
                     /translation="MRSAFMDQMKEAMKAGDKPRVGAIRLIIAALKEKDIEARGAGKG
                     QASDEDILGMMQKMIKQRQESADIYDKGGRPELAAGERAEIAVISSFLPQQMDEAEVK
                     AAIAAAIAATGAASMKDMGKVIAELRGKFAGRMDFGKASGLVKAALGG"
     gene            complement(22907..>23162)
                     /locus_tag="PLQ11_02420"
     CDS             complement(22907..>23162)
                     /locus_tag="PLQ11_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HRK23786.1"
                     /translation="TQITDAAPLAGLTALTTLDLSNTQITDAAPLAGLPALQRIIVGS
                     EKRARALAKTLVRHYRIDHDNTEPIWWEIHLEPLTRPKRP"
BASE COUNT         4051 a         7033 c         7843 g         4235 t
ORIGIN      
        1 catcggccag aggggtggcc ttgcggtggg gccggcgacg cccttgatcc tgcggggcaa
       61 ggcccccgtg cttggctggg caccgccgat gctgccgcgc gccggcgacg atctggccgt
      121 caggctgctg gatctctatc agcaccgtga tccggccctg atgaaatcgc tcgccgctgc
      181 gttggaaacc gacaagatgg cgcgcgccaa cggcatgaat gttggtggcg gtgccaatcc
      241 gcagcgcatg gtgcaggtgg ccgagggcgt ggcgaagctg atggcggcgg cggacgggcc
      301 gcgtgttgct gctctggcct acgagggctg ggatacgcat gccaacgaag gcggggcgaa
      361 gggacggctt gcgacgcttc ttgcgggcct ggacgggtcg tttcaggcgt ttgagcgact
      421 gctggagcct gtctggaagg ataccgccgt gctggtggtc accgaattcg gccgcacggc
      481 gcggatcaac gggaccgagg ggaccgatca cggcacagcc acagtggcat tcctgctcgg
      541 cggcgcggtc aaaggcggca gggtgctggc cgattggccg ggtctcaggc cggcggcgct
      601 gttcgaaggg cgcgacctca ggccgacgac ggatttgcgc gccgttctga agggtgtgct
      661 tgcggatcat ctcggtctcg gtgcgaatgt gctggcgggg gatgtttttc ccgacagcgg
      721 cgcggtcaag ccgctgaccg gactgatagc ctgatcgtaa tctggcgccc tttggcgcct
      781 gtggaccctg ccatgatgct gaaccttacc tcgccggccc tgttgccgat cctgatgctg
      841 atcgcatcca acgtgttcat gaccgtggcc tggtatggcc atctcaagtt caagacctcg
      901 ccgttgtggg ttgcggtgat ggcgagctgg ggcatcgcct tcttcgaata ctggctggcg
      961 gtgccggcca accgcatcgg ccatcaggtc tattcggcag cggaactgaa aacgatgcag
     1021 gaggtgatca ccctggtggt gttcgccgga ttttcggtgt tcgtgctggg cgagatgctg
     1081 acgtggcggc acgctctcgg ctttgcactg atcgccgccg gggccgcgat catttttcac
     1141 ggccggtgag gctttacgcg ccgccgcacg gcagccattc tggcggggct gatcttatcc
     1201 cgggtggtgt catgaacgtt tccgcttcgc cccgcattgc tatttccagc ggtattccag
     1261 ccctcgatgt ctatctcgag cgcgattacg agacggtcaa gggcatgtca tcgcggtttg
     1321 cggcggcgat tgccgggcat ctgatcgcct ttcaggcgcg gcgcgggatt gtcggggata
     1381 tcgccgagat cggcgcgttc aaggggcggt tcttcatcgc catgatgctg gcggccgagg
     1441 cgggggatcg tggcctgggc atcgacgtgt tctcctggcc gagtgatgca acttacgacg
     1501 aatttgtcgc caattgtgct gcgcatggtc tcaaagcgcc gcgtattgat gcgtggaagg
     1561 ccgatacgcg gaccatgggc gtgcgcgatc tgagaaagcg gctgctgggc cgtgccgtgc
     1621 ggttcttcca catcgacggc gaccattccg acatctgcct gaccaaggac ctcgaactcg
     1681 caaccaaagt gatgcatccc gagggcatcc tgtgcctcga cgacatgctg caccccggct
     1741 atccgacgct ggtggcgacc gtgatgcgct atctcaagcg gcatccggag atgggtgtgt
     1801 tctgcgtggt ggatcgggag gatattgtcg cagcgccgaa attcatgctg tgccggcgga
     1861 cgatgaaaga cacctacgaa gccgaactga tgcgggcctt cccgcaattc cactgggttc
     1921 tgggggctga tttcggcgcc gaggactggt gcgtcgtgct cacgccaagc ccgcggctgg
     1981 cggcggtcga ttgacgcccg ctcagcggat cagcagggtt ccgaggccgt tgagatacag
     2041 ggttgcggcg gcaaagatga tgccgatgat cagggacgag acgatgatgc tcagcggcag
     2101 atcgtccttc aggctgtcct tcagcatgcc caatttctcc tcaggtggat tgctgtcgtc
     2161 atcaatcttc ggaatacgca tcacccaacc tcggaatctg gatggcgctg cactggtcca
     2221 ggcccgctgc gccacccgcc actaaattcc gaatcactct ctgttgctgt gtgttggaac
     2281 acatcgcgat ggtggaagcc gggcggcatt cgggccgcat tgtggcaaag gcgcttttcg
     2341 gcgcgacaca ggagccgctt gccttttgtt gcgtgggcgg aaacactcgg gaccgggaga
     2401 gacggggtgg atgtaaagcc ggacaaggcc gggccgggcg ggggcgctcc cgttcaggcg
     2461 acggtgtggc atgatcttgg atggctgttc gccgcgttgc gacaggcgcc gacgcgcggg
     2521 cggattgcgc tgatcgcggc ggccattgtc gtgatcctga tcgccaacat ggtcgcccag
     2581 gtccagctca accgctggaa cggccagttc ttcgatgcgc tcagcgccaa gaatttcgac
     2641 ctgttcctga ccatgaccgg cgtgttcttc atgctgatgg cggtgctgct gtcgctggtg
     2701 gtggcgcaga cgctggcgca ggaactgctg aagatcgaac tcaggcagtt cctgaccacg
     2761 catctggcgg atctgtggct ggcgcgcgcc cggccctacc gactggtgtt cgccggcgac
     2821 atcggcgcca atcccgacca gcgcgtgcag gaggatacgc gcaagctggc cgaactggcg
     2881 gccgagcttg tcgtcggcat gacccagcag atcttcctgc tgacgacctt catcggcgtg
     2941 ctgtggacgc tttcctcgga actgaaattc tggatcggcg ggcaggaaat ctacatcccc
     3001 ggctacatgg tctggtgcgc gctgatctat gcagggcttg gctcgctgtt cgccttcctc
     3061 gtcggccggc cgctggtcag gctgaatgcg gagcgctcgc agaaggaggc agaactgcgt
     3121 tttgcgctgg tgcggctgac cgaaagtgcc gagagcatcg gctttctcaa cggcgaggct
     3181 gacgaacggc gcaatctcaa ccagccgatc ggcgcggtga tcggcatcct caggcagatc
     3241 gcattcaagc tggcgcggct gacctgggtc acctccggtt atggctggct gtcgctgatc
     3301 gtgccggtgc tggtggcggc gcccggatat ttctccggcg cggtgacatt cggcgggctg
     3361 atgcggctga tcgatgcctt cacccaggta caggcttcgc tgcgctggct ggtcgacaac
     3421 gttgccaaga tcgccgattg ccgcgccgcg ctctaccggg tgatgcggtt cgaggaggaa
     3481 ttgcgcacct cgcatctgga agaggccgag ttgaaaggca tccatgtcga agccgggggg
     3541 gcgggcaggc tggagctgga gggcgtccgc gtgaccatgc cgaacggccg gccggtgatc
     3601 gcggcggcct cgttcaggct ggctccgggc gagcgggtgc aactcgaagg gctggccggc
     3661 accgggaagt ccaccttgct gcgcgcgatt tccggtctgt ggccgtgggg tggcggcacc
     3721 atccgtcggc cggacgccga ggggctgatg ttcctgccgc cgcgccccta tctgccgctc
     3781 ggctcgctgc gcgaggcggt gtgctatccc gatccgccgg agcgcttctc cgaggcggcg
     3841 gtgcaatcgg ccatgcagcg ctgcggcctc gggggccgca tcgaccggct cgaccatgtg
     3901 cggcgatggg atcgcgacct gaccatcggc gagcagcagc ggctgacctt tgcgcggctg
     3961 atcctgcatc ggccggcgct ggtgatcatg gacgaggcga cctcggcgct ggacgagccg
     4021 gggcaggccg ggctgatccg cctgttcgac gaggagctga aggactcggc ggtcctgttc
     4081 gtgtcgcatc gcggcggcta tggcgggctg gcagagcggc ggatgcggct ggagccggga
     4141 gcagaagggt cggttctggt cgattctgtg cgctgaagcg gcaattggcc aactggcagg
     4201 ttcgggatgc aggcggtatc cttgtgcggg tgattcacat tgaaaggtca tcggatatgc
     4261 tgcgcattct tggtgtcttg atcgcgctgg cagcagccgg agcagcagag gctcaggaca
     4321 gtggccgcga tattcgtctg ggcaagcgca tcgccgagaa gaactgcgcc aattgccatt
     4381 ccatcggcct caagggcgca tcgacgcgca agggcgcgcc gccgttccgc acgctgcacc
     4441 ggcgctatga tctcgacaat ctgcaggaag cgctggtgga gggcgtcgcg gtcggccatc
     4501 gcggtgccga catgccggaa ttcaccttca cccccgacca gaccgacgct ctgatcgcct
     4561 atctgaagtc gctcaagggg cgttagagaa ttttcaggcg agttggagcc cggttcgcgt
     4621 caagaaaatg cgaaagaaca aaaagatgga gcgcatttac gtgaattgga attcgctgaa
     4681 agtgctctag ttctgattgc cgcttgccag cgaggcggcg cttgtgcatc acagcgccat
     4741 gacctcctgg gtggatttct ggaaccgcga cccctcgatc tatgtgaacg agcggcacaa
     4801 gatgctgcac taccggcagg tggcgcgcga catcgccggc ctgatcgaca acgggcaggc
     4861 gcgcgtgctc gatcacggtt cgggagaggc gacgtccgcc gatctggttg cggcgcgctg
     4921 cgccgaactc gtgctgtgcg atgccgcgcc caacacccgc gcccgcatcg cggcgcagtt
     4981 tgccggcaag gacacgatcc gcgccatggc gccggaggat ctgccgtcgc tgccggacgg
     5041 gcatttcgac ctgatcgtcg ccaattcggt gctgcaatat ctcgacgagg tgacgcttga
     5101 ggcatcgctt gaggaatgga agcggttgct caagccggac gggcggctga tcctcgccga
     5161 tgtcattccg ccgaatgtct cgccgctggc cgatgccagg gcgctgctgg gctttgccat
     5221 gacgggcggg tttctggtcg cggcgatcgg cgggctgatc cgcacggcgc tgtccgatta
     5281 tcgcaagctc aggagcgaac tgggattttc cacccattcc gaggccgatc tggtcgcccg
     5341 gctgcagcgc cacggatttg ccgtgcggcg gctcgatcac aacatcggcc acaacccggc
     5401 gcggatgagt ttcgaggcgc ggccggtctc agagtcctga gggcggttcg gggcgtgtca
     5461 cgatgaaggt cgccgcaccg gccagacaga cagcctggat caggatcacc agcggcttga
     5521 gctgaagcag cagggatatc gccagaacgg cggccatcga gccacaggcc agccatttgg
     5581 tgcggcgggc gatggcgcca tgcgcctgcc agtgccgcac cggcgggccg aaggtcgggt
     5641 gttcgacgat ccagcgatgc aggcgcggcg aggaccgggc aaaacaatag gccgcaacca
     5701 acatgaacgg cgtggtcggc aggatcggca gcactgcccc ggcgatgccg aggccgaccg
     5761 cgcaccagcc gagggcgagc cagaggagct gcgtcacgcg cggtccccga aagagaaata
     5821 cttgtgggca aagaaactcc acaccagcac gaggccggtg gtgacgatct gcgccggcag
     5881 gtagggcagg ctcatgcgct cgacgaacag gctcatcagc ccgtaggtca acacaaatcc
     5941 gacgccggcg acgaccgcaa agcgccagcc ggcctcctca tgcggccgct cggaccggta
     6001 agtgtggcgg cggttgagca ggtaggaaac gatgccgccg aggatgtagc cggccagcgt
     6061 ggccggcacc gcgcgcaggc caaatccttc aaccccggcg atcagcacgc cgtaatgcac
     6121 gatggcggcg acaaggccga caccgaaaaa ggcgacgaac tggcggagaa gcgcgaacat
     6181 gcagcgggca tagccggttt cgaccggcta ggcgagcgca tcgacgcgat ccgggaaggg
     6241 agctggggtc tcaaagctgg tgtttcggca ggtgattcct ggcgaatgcc cctccccctg
     6301 ccccgcccct caccctgaac ctctccccgc tagcggggag agggagatat tggggacgat
     6361 cgcagctttc ggccgctgac cttcttccca atggcggaga gagggagttt ttcaaggaac
     6421 gtcccttctc cctctccccg ctagcgggga gagggttggg gtgaggggcg gggcgctgtg
     6481 aggggccacc ccgagttatc cacggcccgc attcacccca ttctgttgca cctgccgctt
     6541 ttccctttcc ctcggcgcct tgcccggcta tagcggcgct ttagcaaact gaacgacttt
     6601 ccctgccgcg cgccgtttca ctgaaacggg gttccgcgag gccgaccatg cacgctgaaa
     6661 cgaccctgaa atgccaaaga gaaccgacct tcacaccatt ctcatcatcg gcgccggccc
     6721 gatcgtcatc ggccaggcct gcgagttcga ctattccgga acgcaggcgg tcaaggcgct
     6781 gaaggccgaa ggctaccgca tcgttctggt caattccaac cccgccacga tcatgaccga
     6841 tcccgatatg gcggatgcga cctatgtcga gccgatcacc gcggagattg tcgccaagat
     6901 catcgaaaag gagcgcggcg accggtcgaa gggctttgcc gtgctgccga ccatgggcgg
     6961 gcagacggcg ctcaactgcg cgctgtcgct caaccgcaag accctcgatg acggcaaggg
     7021 cggcaagacc tcggtgctgg agaagttcgg cgtcgagctg atcggggcga ccgcgcatgc
     7081 catcgacaag gccgaggatc gcgaattgtt ccgcgaggcg atgaccaaaa tcggtctgga
     7141 gacgccgcgc tcgcaccatg tcaagacgct cggggcggcg ttggaggcgc tgaaggatat
     7201 cggcctgccg gcgatcatcc ggccctcgtt caccatgggc ggcaccggcg gcggcatcgc
     7261 ctacaacaag gaagagttca tcgacatcgt cgagcgcggc atcgacgctt cccccacctc
     7321 cgaagtcctg atcgaggaaa gcgtcctcgg ctggaaggaa tacgagatgg aggtggtgcg
     7381 cgataaaaac gacaactgca tcatcgtctg ctcgatcgag aacatcgacc cgatgggtgt
     7441 gcacactggc gattcgatta ccgtcgcgcc ggcgttgacc ctcaccgaca aggaatatca
     7501 gcgcatgcgc gacgcctcgc tggcggtgct gcgcgagatc ggcgtcgaga ccggcggctc
     7561 gaacgtgcag tttgccgtca atcctgccga cgggcggatg atcgtcatcg agatgaaccc
     7621 gcgcgtgtcg cgctcctcgg cgctggcctc gaaggcgacg ggctttccga tcgccaaggt
     7681 cgcggcgcgg ctggcagtcg gctacacgct cgacgagatc gccaacgaca tcaccggcgg
     7741 cgccaccccg gcttcgttcg agccgaccat cgactatgtg gtcaccaaga ttccgcgctt
     7801 tgccttcgag aaattccccg gcgccgatcc ggtgctgacc acctcgatga aatcggtcgg
     7861 cgaggcgatg gcgatcggcc ggacgttcca tgaatcgctg caaaaggcgc tgcgcagcct
     7921 ggagaccggg ctgaccggtc ttgatgacat cgagattgag gggctgggca ggggcgagga
     7981 tcacaacgcc atccgcaagg ccatcggcac gccgacgccg gaccggctgt tgcaggtggc
     8041 gcaggcgatg cgcgtcggct tcagccttga ggacattcat gcgtcctgcc ggatcgatcc
     8101 gtggttcctc gaacggttga aagaaatcgt cgacatggag gcgatggtgc gggcgcatgg
     8161 cctgccgacc acgcctggcg cattccgcca gatcaaggcg atgggctttt ccgacaagcg
     8221 gctggcgaca ctggtgaaat cgaccgaggt cgaggtgcgg gccgcgcgcc gcgcgctcgg
     8281 ggtgcggccg gtctacaagc gcatcgacac ctgcgcggcg gagttcgctt cgccgaccgc
     8341 ctacatgtat tcgacctacg aggcgccgtt tgcctgtgcg ccggccgacg aggcgcggcc
     8401 atcgaatgcc cgcaaggcgg tgatcctcgg cggcgggccg aaccgtatcg gccagggcat
     8461 agaattcgac tattgctgct gccatgcctg tttcgcgctg gcggaggccg gctacgagac
     8521 gatcatggtc aactgcaacc cggagaccgt ttcgaccgac tacgatacct cggaccggct
     8581 gtatttcgag ccgctgaccg aggaggatgt gctcgagatc atggccaagg agatggaggc
     8641 cggcgagctt gccggcgtca tcgtccagtt cggtggccag acaccgctga agctggccga
     8701 ggctctcgaa gaaaatgaca ttccgatcct cggcacatcg ccggatgcga tcgacctggc
     8761 cgaggatcgc gaccggttca agcgcctctt ggacaagctc aagctccggc agccgcagaa
     8821 cggcatcgcc tattcggtgg aaaaggcccg tgacgaggcc gagaagctcg ggtttccgtt
     8881 cgtggtgcgg ccatcctacg tgctgggtgg gcgggcgatg gcgatcatcc gcgaccggga
     8941 agcgttcgac gattacctgc tcggcacatt gccgagtctc attccgtccg acatcaaggc
     9001 gcgctatccc aacgacaaga ccgggcagat caacacggtt ctggggcgca atccgctcct
     9061 gttcgaccgc tatctgaccg atgcgatcga ggtggacgtc gattgtctgg cggatggcaa
     9121 ggatgcgttc atctgcggca tcatggagca tatcgaggag gccggtatcc attcgggcga
     9181 ttcggcctgc tcgctgccgg cccggtcgct gtcggccgaa acgatcgccg aactggagcg
     9241 gcagacgcgc gccctggcgc tggcgctcga tgtcgtcgga ctgatgaacg tgcaatatgc
     9301 catcaaggat ggcgtcatct acgtgctgga ggtgaacccg cgcgcctccc gcacggtgcc
     9361 gttcgtcgcc aaggtggtgg gcacgccctt cgccaagatc gctgcccgcg tcatggccgg
     9421 tgaaaagctc aggagtttcg atctgaagtc cgagccgtat gatcatgtcg gcgtcaagga
     9481 ggcggtgttc ccgttcaacc ggttcccggg cgtcgatacc gtgttggggc cggagatgcg
     9541 ctcgaccggc gaggtgatgg ggctcgatcg ctcgttcggt gtcgccttcg ccaagagcca
     9601 gctcggggcg ggcagcaagg tgccgaaatc cggaaccgtc tttgtttcgg tgcgcgacga
     9661 cgacaagccg cgcctgctgg aatcgatccg cgagcttgtg cgcctcggct tccgcatcat
     9721 cgccacaggc ggcacacagc gctatctggt cgagcagggg gtcaaatgcg agcgcatcaa
     9781 caaggtgctt gaaggccggc cgcatatcgt cgacgagatc aagaacgggc gcgtgcaact
     9841 ggtgttcaac accaccgatg gcgcccaggc gatggctgac agtaaggaac tgaggcggca
     9901 ggccctgctc gccaaggttc cctattacac cacactggcg ggcgcagtgg ccgcggccga
     9961 aggcattgcg gcctatctcg gcggcgatct cgaagtgcgg gcgctgcagg attatgcccg
    10021 atagcccgcc cttttatcgc acttgcacgc aatggcgaat tgaatcactc tgccttgcga
    10081 gcctgtggcg acgccgcccc ggcggcggcc tacttctatg aatggataga ccatggaacg
    10141 tgtcccgtta acgcctcagg ggttcgccgc gctggaagcg gagttgaagc gccgccagca
    10201 ggaagagcgg ccgcgcatta tcctggccat ccaggaagcg cgggcccatg gcgatctttc
    10261 ggagaatgcc gaatattcct cggccaagga gcagcaggcg ctcaacgagg ggcgcatcac
    10321 cgaactggag agcctgatct cgcgcgccga tgtcatcgac atatcgaagc tgagcggcag
    10381 caaggtgaaa ttcggcgcca ccgtgaccct ggtggatgaa gagaccgaga aagagcgcgc
    10441 ctacatgatt gttggcgaga ccgaggccga tgtgaagctt gggcgggtgt cgatctcctc
    10501 gccgatcgcg cgggcgctga ttggcaaatc ggtcggcgac agtgtcgagg tcaacacgcc
    10561 cggcggcgcc aagagctacg agatcgtcaa agtcgccttc cggtgacgca gggccgtttt
    10621 ccctctcgac tcgcaagctg cgtcacgcca tagtgttcag gtgtgaacaa aaggcgtcta
    10681 ccggcagcga atgccggtgc tttcggggag gaaagcaatg agcgcgaaga ccgcattgga
    10741 tcggccgtgg ttgcgtcatt atccggccgg tatgcctcat gaaattgatg ttgccgcagg
    10801 gggcacgctt gccgatatcc tgagggattg cgccaagcgg tatgccgaca agccggcatt
    10861 cgagagcttt gaaaagaagc tgagctttgc cgagatgttc gccatggccg aggctgtggc
    10921 gtcctggctg catcagcagg gctgtgtgaa gggcgagcgt atcggcctga tgatgccgaa
    10981 tgtgatggcc tatccggcct cgatcttcgg cgtcatcctg tcgggctgca cggtcgtcaa
    11041 catcaatccg ctctacacgg tgcgcgagct caaccaccaa ctcaaggatt ccggcgcgac
    11101 cacgctgttc gtgctcgaaa attttgccag tgtcgcgcag gatggcatca agggcaccaa
    11161 cgtcaagcgc gtggtgatcg tcaagccggg tgatctgctc ggcctcaagg gcaagatcat
    11221 caatctggtg tccaaatatg tgaagaaggc ggtgccggcc tggtccatcc ccggcacgat
    11281 cgcgttttcc gatgtgatcg cggccgggcg ttccaagccg atgaatgccg tcaccatcac
    11341 ccaggatgat gtggcgttcc tgcaatatac cggcggaacc accggcgttt ccaagggtgc
    11401 gacgctgctg catcgcaatg tcgtcgccaa tgtcctgcaa tgcgacatgt ggctgtcgcc
    11461 gaaaatgcag ggtgagccga accacgtcat ggtgacggcg ctgccgctgt atcacatctt
    11521 cgggttgacc tgctgctgcg tgatgatgtg gcgcatgggg gcgatgtgcc tgctgattgc
    11581 caatccgcgc gatttcgccg gctttatcgc catcatcaag aagtgccgcg tcacgctgtt
    11641 ttcgggcgtc aacacgcttt acaacgcgct cgccaatcac ccggacatca agggcgtcaa
    11701 tttctcctcg ctgaagatct cggtgtctgg cggcatggcg gtccagtcgg ctgtggcgcg
    11761 caagtggaag gagctgaccg gaacggcgat cctcgaaggc tatggactgt cggagacctc
    11821 gccggtggtg acgctcaatg atccgttcct cgaagagttc tcgggcacga tcggctttcc
    11881 ggttgcgtcg accgatgtgg caatccgcga cgaggcgggg caagacctgc ccgccggcca
    11941 gtcgggcgaa ctctgcgtgc gtggcccaca ggtgatggcc ggctattggc agcgcccgga
    12001 cgagacggcc aagacgatga cggcggacgg gttcttccgc accggtgaca ttgcgatgat
    12061 gctgccggac ggccagttca agatcgtcga tcgcctcaag gacatgatcc tggtgtcggg
    12121 cttcaacgtc tatcccaacg aggttgaaga tgtgctcgcc agccatcccg gcgtgctcga
    12181 ggtggcggtg atcggcgtgc cggatgccgg ctctggcgag gcagtggccg cctatgtggt
    12241 caagaaggat ccggagctga ccgccgatgc cgttcgggcc ttcgccaagg aaaagctgac
    12301 cgcctacaag gtgcccaagc aggtgcattt ccgcgattcc ttgcccaaga ccaatgtcgg
    12361 caagctgttg cggcgtgtgc tgcgcgagga agtcctcggc aagagctgag ccctgatcgc
    12421 gggtttttga cttgctgtga gcagagctga gagcgaagag cagttggctt gcgtatgaag
    12481 ccagccgctc aagccttcag ggctgactca cacaggaatc ctgcaatgct catccgccgc
    12541 cctcactcgt gggaattgcc cgaatcggcg gtaacgcccg atggcgttgc ccccagccgc
    12601 cgccagatgc tcggcggtgg tctggcgttg ctggcttctg catctgcggc aggggcgcag
    12661 cgggtcgatg atgatccgtc gatggtgctg tatccctttg cgcgcaatga tgcctacccg
    12721 cctcgcggca aggtgaccga cgagacgctg gtcgccaatt acaacaactt catcgaattc
    12781 ggctcgggca agcggatcca gcgcgccgcg caggcgctgg tggtgcggcc gtggaccatc
    12841 aggatcgatg ggcaggtgga aaagccgatc gagatgggga ttgatgatct gctgcggctg
    12901 ctgccgcgcg aggagcgcat ctatcgccat cgttgcgtcg agacctgggc caaggtggtg
    12961 ccgtggagcg gggtgccgct caaggcgctg ctcgaccatg tgcggccgct ggcctcggcg
    13021 aaatacgttc gctttgaaac gtttcacgat ccgaaaatgg cgccgggaca gcgctcgccg
    13081 gtctatggct ttccctattc ggaggccatc accatcgcgg aagcggccaa tgaactggcg
    13141 tttctggcaa ccggcgccta tggcaagccg ctgaaaaagc agttcggggc accggttcgg
    13201 ctggttctgc cgtggaaata cgggttcaag agcgccaaat ccgtggtgcg catcagcttt
    13261 gtggaaaagc ggccggtcag cctgtgggaa aaactgcaac cggatgagta cggcttctgg
    13321 gccaatgtga acccggatgt gccgcatgtg cgctggtccc aggaagagga ggaggtgctc
    13381 ggctcctact cccgcaccaa gacattgatt ttcaatggct atgccgaaca ggtggcagcc
    13441 ctctaccgtg ggctggagaa cgaaaagctg ttcacctaga gcattttcaa gcgaagtggg
    13501 ttccggttcg cgtcaagaaa atgcgaaaac aagaagaaga gcgctttttt agagcatgct
    13561 gccatcgggt ggatgctggc gcgtcgtgac cagcatgctc tgatcacttg ataacgcgca
    13621 caaaaaacaa ggccggatac ctcgactaca ttacatgtcg aggtatccgg cttaagaata
    13681 cgctccacgg gaggagaaga gcgtataacg ccagaggggc tctgcaccgg gaggagatgt
    13741 gtggccgtcg ctctgacaac ttagatatcg ttgattcttt tgcgacgcac aatttaataa
    13801 ttggtatagg tcatatgcgc gcacggcatg gctcgccacc atgccggatc atgctcctgt
    13861 ttcatgtgtt gccgcgtttt cttcacgcga accgggttcc acttcgcttg aaaatgctct
    13921 aggcaatgcc ggcgcaggcc cagcgaatgg ctattgcggc tcgaccttgg cggccttgat
    13981 caccgcttcc catttgagca tttccgcctt gtggtaggtt gcaaaggcgg cgggttcgcg
    14041 cagcgacagg gcaaagccga gtttcaccac gccttcggcg accgccggct tgccgagcgc
    14101 gcccagcacc gccttggtca gccggtcgcg cgcctcgggc ggcgttgcgg ccggggaaaa
    14161 cagggcgagc cagctgtccg cttcggaatc gaccaagccc tgttccttca gcgttgggac
    14221 atcgggcagt tgcggggcgc ggttgaggct ggctaccgcc agggcgcgca tagagccgcc
    14281 ccggatctgt ccgagaacac tcggcagcgt ggcaaaggcc agttcgatcc gcccggccat
    14341 cacctcggcg gccatggggc cggcgccctg ataggggata tgggtcatgt cggtgccggt
    14401 gcgggtcttg aacagttcca tcgccagatg cgagccggag ccgatgccgg tggacccgta
    14461 gttgaacttg ccggggtcct ttttggccaa agcgaccagt tcggagacct tggtggccgc
    14521 cagagcattg gtcaccacca gcacatgctg aaggtcggca aggccggcga ggggagcgaa
    14581 atccttgatc ggatcgtagc cggcctcctt gaccagaaag gcgttgttga cgtgggtctg
    14641 gttgttgccg agcacgatcg tatgcccgtc ggtctcgcct ctggcgacgc cgcgcgtgcc
    14701 gatggaaccg gcgccgccgg gcttgttctc gacgatcacg gtctggccga gatcgccctg
    14761 gatttcattg gcgatgatgc gcgccagcgc gtcggtcggc ccgcccggcg gataggggac
    14821 aacgatgcgg acaaccctgt tgggaaagcc ttgtgcgctg gccatctgcg gcagggcaag
    14881 ggccgctccg gcggcggcca gccaggagcg gcggttgagg tgagtgctca tgcggatcaa
    14941 cccttcttgc atcggcgcga gcccctgccc gcattttccc ttgaagcgac gggcgggctt
    15001 gcctaaggaa gtccgtccct tgtcggacaa gtttgcataa gctggagccg ttcgccatgg
    15061 cctttcttgc cgatatcctg tctgtcgtga agccctcggc caccatcgtc ctgacccaga
    15121 tggcgcgcga gctcaaggcc aagggcaagg atgtcatctc gctgtcggtc ggcgagcccg
    15181 atttcgacac accgcagaac atcaaggatg cggcgattgc cgccatccag cgcggcgaga
    15241 ccaaatacac cccggtggcc ggcatccagc cactcaggga ggcgatcgca gccaagttca
    15301 agcgcgagaa cgggctgacc tacaagccga gccagatcat cgtcggcacc ggtggcaagc
    15361 agatcctgtt caacgcgttc atggctaccc tgaacaaggg cgatgaggtg atcattccgg
    15421 cgccctactg ggtcagctat ccggaaatgg tggcgctgtg cggcggcacg ccggtgtcgg
    15481 tgctcaccag gcccgagaac aagtacaagc tgcaggcgga cgagcttgag aaggcgatca
    15541 cgccgcgaac caaatggctg gtgttcaact cgccgtctaa cccgaccggc ggcgcctata
    15601 cccgcgccga actcagggcg ctgaccgatg tgctcatcaa gcatccgcat gtgtgggtgc
    15661 tgactgacga tatgtatgaa catctcacct tcggcgattt cgtgttcacg acgccggctc
    15721 aggtggagcc ggcactgtat gaccggacgc tgacgatgaa cggggtgtcc aaggcctatg
    15781 ccatgaccgg ctggcgcatc ggctatgccg gtgggccgga agcgctgatc aaggcgatgg
    15841 acatggtgca gggtcagcag acctcgggcg cctgctcgat cgcccaatgg gcggcggtgg
    15901 aggcgctgaa cgggccgcag gacttcatcc cgatgtccaa gaaggtgttc gagcagcgcc
    15961 gcgatctcgt cgtctcgatg ctcaaccagt gccgcggcat cacctgcgcg acgcccgagg
    16021 gtgcattcta cgtctatccc tcgtgcaggg atctgattgg tctcaaggcg ccgtcgggca
    16081 aggtcattgc gaccgacgag gacttcgtga tggagcttct gaacgaggag ggcgtcgcca
    16141 cggtgcacgg ctcgtcgttc ggtctggggc cgaatttccg ggtttcctac gccacctcca
    16201 acgacaagct ggaagaggcc tgccggcgca ttcagcggtt ctgcaacgcc ctgacatgaa
    16261 gcctctgcct ttgtttgggc acaattggcg ccgagacggg accgcacgat gtcgtgtagg
    16321 gcctgttcag gcgtggagaa gcttttgacc agggcatgca ggtctgcatg gattgccgga
    16381 attctggcgc ttgctgtttc ggcgagcctg ccggcgcatg cgcaggggcc ggctctgtct
    16441 ctgctgccgc aggaccagcc cggccggtcg gctgcaccat ggtcggcttt cagatttcct
    16501 gttgccccgg cgcccagtcc gcagagcgaa agcgtcgagg ccgagccgga gtcagccgag
    16561 gccgcagagc ccgcaaaagc ggccgatccg tcccgatcgc caaaggcgac acaggcgccg
    16621 gccagctctc agccgaaact ggcgaatgtt cccttgccgc cgaggcccga ttctgccttg
    16681 cgggcggcgg ctgccaagga ggacgccgca aaagcgcggc tttccggggc cgcctccgcc
    16741 ccggctgcga aggcggaacc ggaaccgcca gcgcgggcaa ccgggatctt cgccctgctt
    16801 tctggaacag gcgccccgtc ggtcgatgcg ccggccaatg atgcggccga gcagagcatt
    16861 ctggtccgag cggttggcgc cgcaccggac cggccggtcg aagggccgtc gtccagggcc
    16921 acgcaggtca cgcgcctgga cctctccaac ccgtttcttc ccaagccgat gtcgccgtcc
    16981 gacgaagagg gactggacga tgaggaggcc tccaagattg ccgagaagca ggtggattcg
    17041 gtgcagatcg cctgcctcaa accgtcgttg atggcgatga ttcagaaggc cggcgagcat
    17101 tttggcgcaa agccggtgat tacctcgggt ttccggccct atgggcggcg gggttcctat
    17161 catcgcagat gcgaggcggc ggatttcttc attcccgatg ttccggcggg caggctcgtt
    17221 cagtttctgc gcaatctgcc gggggccggc ggggtcggca cctattgtca taccaagtcg
    17281 gtgcatctcg acacgggcga gccgcgcaac tggcatcaat gcggcttccg gcgcagtttc
    17341 gcgttgcgtg tgccggcgct tgccgaaaac gggtcgcgct gagcgcaggc ccggaccaaa
    17401 ctggctgcga ccttgcagcg aggcgtccca tgacgacatt tctcaccacg acatttctca
    17461 ccgatgactc ccgctgggcc gcgttcgtgg cgcgcgatcc tgcggcggat ggcgcgtttg
    17521 tggtcggggt ggtgacgacc gggattgtct gccgcccggt ctgtgccgcg cggccgaaac
    17581 gggagaacgt gcgcttttac gaaaccccag ccaaggccat cagcgccggc tttcgcccct
    17641 gcaagcgctg ccggcctgtg ggttgagggc acggcatggg ttgcggggcg gcgaaaaacg
    17701 cgcttcaagc gttgccgtgc cggacccgat gatgcctccc agttccgttc ccgatcttgc
    17761 cctggctcgt gccggcgtgg cgggcgcccg cgtctgggtt ctttccgatg gcaaggcggg
    17821 cgacgaggtg cagtgcctgg gggttgcaga gcggctcggc ctggagccgg agctgcggcg
    17881 cgtgaagccg cgcgcgccct atctctggct gatgccgtgg ggcggcattg acccggccga
    17941 ggcgccgggg cggcccggca gtccgttggc cggtcccttg ccggatatcg ccattgcctc
    18001 cggacgccgc acggcaagct atctgcgcaa ggtcaaggcg ctgtcgggcg ggcgctgctt
    18061 caccgtgttc ctgaaggatc cgcgctgcgg cgccggaaca gccgatttcc tctgggtgcc
    18121 ggagcatgac cggctacgtg gcgccaatgt gctggccacg ctgacctctc cgcacagaat
    18181 cagtccggaa cgactggccg cagcacggac gaatccgcct tatggcctcg accggttgcc
    18241 ggggccgcgc gtcgcggtgc tgatcggcgg tgacagcgcg catcatcgct tcacgccggg
    18301 cgacattgca acattgctcg cccattgcac gcggctggcg gagaccggcg cgacgctgat
    18361 ggcgaccgcc tcgcgacgga caccgccggc gctggcggga gcgctgacgg aactggtgcg
    18421 cggcagcggc ggcttcttct gggacgggac gggcgagaac ccctatatcg ctctgctggc
    18481 gctggccgat gcggtggttg tgactgccga ttcggtcaat atggccggcg aggcggcggc
    18541 gaccggcaag ccggtcctgc tgttcgaacc ctccggcggc agcgacaaga tcacgcgctt
    18601 cctgcgccag cttgaacggg ctggcgtcgt ccaccggttt gacggaacca tcgccggatc
    18661 cccatatcag ccaatcgact ccaccccggc gattgccgcc gaactggccc ggcgctatct
    18721 cctccaccgc cgcgcccttt cctgaacgag agcagccatg tccaccgcac ccagccacac
    18781 caaggtcctg atcatcggct ccggcccggc cggctatacg gcggcgatct atgccgcccg
    18841 cgcgctgctc aagccggtga tgatctccgg tttcgaaccg ggcgggcagt tgatgatcac
    18901 caccgatgtc gagaactatc cgggctttgc cgatgtgatc caggggccgt ggctgatgga
    18961 acagatgcgg ctgcaggccg agcatgtcgg cacggagatg atctccgatc acgtcacgaa
    19021 ggtggagctt ggccagcggc ctttcgcggt gacctgcgat tccggcgcca ggtttaccgc
    19081 cgatgcgctg atcatcgcca ccggcgccaa ggccaaatgg ctgggcctgc catcggagga
    19141 aagcttcaag ggctatgggg tctcggcctg cgccacctgc gacgggttct tctatcgcgg
    19201 caaggatgtg gtggtggtcg gcggcggcaa ttcggcggtg gaagaagcgc tgtatctcgc
    19261 caatctcgcc agttcggtga cgctggtgca tcggcgcgat acgctgaagg ccgagaaaat
    19321 cctgcaggac cggctgttca agcatccgaa gctcaggttc ctctgggaca gcgcggtgga
    19381 ggaaatctgc ggcgaaaaag acccgccggg cgtcacccat ctcaggatcc gcaacgtcaa
    19441 gaccggcgcg ttgtcggacc acaggacgca cgggctgttc gtcgccatcg gtcatgcgcc
    19501 ggcctcggaa ctgttcgccg ggcaggtgac gctgaagccg tcgggctaca tcgccaccgt
    19561 gcccggatcg accgcgacca acattcccgg cgtgttcgcg gccggcgatg tcgccgacga
    19621 gacctatcgg caggcggtga cggcggcggg catgggatgc atggcggcgc tggaagccga
    19681 acgctggctg catgcgcagg ccggggcctg atgcgccgac cgtgacattc tgacacagat
    19741 tgtgcttgcc tttccggcgt gggcttgcca cggtcggcaa accaaaggga ggcggcatcg
    19801 aaggggaacg cgccacatcg ggaggcgtgg cgcaatgtga ggccctgtga ttccgtttga
    19861 gggatggatt gggacaaggt tcggattttt catgctgcgg cggctgccgg cagcctgacc
    19921 aaggctggtg acgttctggg cctcagccag tcggcggtga gccggcaggt cagcgcgctc
    19981 gaaggtgagc tcaaggttcc gctgttccat cgccatgcgc gcggactggt cctgacggag
    20041 cagggcgagc tgttgttccg cgccgcccgc gatctggtgc agaagctcga acagacccag
    20101 gcgcagctga tggacagccg gcagaaaccg gacgggttgc tgaaggttac cgccaatgtt
    20161 gggctcggcg cgcattggct gacgccacga ctcgaggagt tcatcgagca atatcccgac
    20221 atcaggctgg agatcatcct gaccgatgac gaactcgatc tggcgatgcg cgaggccgat
    20281 gtcgccttgc gcctgcgcga accgattcag ggcgatctga tccgccgcaa gctgttcacc
    20341 gtgcatttcc acgcttttgc ctcgccggac tatctcaagc gctacggcca gccgcgcctg
    20401 ccggtcgatc tcgaccagca tcggctgctg gcgttcggcg gcgacattcc ttcctatctg
    20461 aagaacctca actggcacct gaccgcaggg cgcgacgcca gcgggccgcg cgtgccggcg
    20521 ctgacggtca acagcctcac cagcctgatg cgcggcgccc agcgcaacgc cggcatcgcc
    20581 gtgctgcctg actatctggt cgagccgaac accggcctcg tgcaggtgct cgcctccgaa
    20641 tcaatgccgc agctcgaatg cttcctggtg ttcgccgaag cgctgaaggg gggcgcgcgc
    20701 gtccaggcat tccgcgactt catcgtcgcc aaggcccagc gctgggcgta ctaagtggcc
    20761 cttgccgtag gcaagggcgt ccctcgcatc agctcggcgt cgctttgctc ctacgccttc
    20821 ggcgaggcaa aggcgcccct cgcatcagct cggcgtcgcg ttgctcctac gccttcggcg
    20881 gggcaaaggc gcccctcgca tcagctcggc gtcgcttcgc tcctatggct gggcgtttca
    20941 gcttatccct cccccttgtg gggagggtgg tccgagcatg gcagtcaagt ttacgcagtc
    21001 tgcggaaact tgactgcgtg tggaggaccg ggtgggggtg gtgctgcaaa atctggcgtg
    21061 cggaagtccg gatcatccac ccccatcccc gacccttccc cgcaaggggg aagggggggc
    21121 accctaacct ggcgctcacg ccgccttttt ggctgcctgc atcagcgtca cgaaccggtc
    21181 gaacagatag tggctgtcgc ggggcccggg cgaggcttcg gggtggtgct gcaccgagaa
    21241 cgccgggcga tccttgagct tcagcccgca attggagcca tcgaacagcg agacatgcgt
    21301 ctcctcggca tgggccggca gcgttgccgg atcgacagcg aagccgtggt tcatcgagac
    21361 gatctcgacc ttgccggtgg catgctcctt caccggatgg ttggcgccgt gatggccctg
    21421 cggcattttc agggttttgg cgccgagcgc cagcgccagc atctggtggc cgaggcagat
    21481 gccgaaggtc ggcaccttcg catcgagcag cgccttgatg gtcggcacgg agtattcgcc
    21541 cgtcgccgcc ggatcgccgg ggccgttggc gaggaagatg ccatcggggc gcatcgccag
    21601 aatgtccgcc gccggcatgg tcggcggcac gacggtgacc ttgcagccgc gatcggcgag
    21661 caggcgcagg atgttgcgct tgacgccata atcgatggcg atgacgttga acgagggctt
    21721 gtcctgccgg ccatagccgc ggccccacag ccagtcggtc tcgtcccagc cgaaacgctg
    21781 ggtggtggcg acggccggca ccagatcctg cccgtcgatc gagggcaccg cgcgggcggc
    21841 agctttcagc gcggcaagat tgaagttgcc gctcggatca tgggcgatga cggcattggg
    21901 catgcccttg tcgcggatca gcgcggtgag cgcgcgggta tcgacgccgg tgatggcgat
    21961 gatgttgcgc gctttcagcc aggcatcgaa atgtctggcc gagcgatagt tggaggggtt
    22021 ggtgatcggc gattgcagca ccgcgccgcg cacgccactt tcggacgcca tgttgacggt
    22081 ctcgacgtcc tcgtcgttga cgccgacatt gccgatatgg ggaaaggtga aggtgatgat
    22141 ctggccggtg taggagggat cggtgaggat ctcctgatag ccggtcatgg cggtgttgaa
    22201 gcagacctcg ccggtggccg agccgatggc gccggcgccg tgaccctcga tcaccgtgcc
    22261 atcggccagc acgagaacgg cggtgggttt gggttcgttc cagccggttg cggtgctcat
    22321 gcggtgctcc atgcgcggca aaagggccgg atgcgtgacg gcatccgttg cccgttgcaa
    22381 agtttgtctc taaagccaag cgacctagac cgtggcggcg ccgaggtcaa cgccaacccg
    22441 gcgccggcaa caggcttttt atcgaaaggg acagacatgc gcagcgcatt catggatcag
    22501 atgaaggaag cgatgaaggc cggcgacaag ccgcgcgtgg gcgccatccg gctgatcatt
    22561 gccgcgctga aggaaaagga catcgaggcg cgcggggccg gcaaggggca ggcgagcgac
    22621 gaggacatcc tcggcatgat gcagaagatg atcaagcagc ggcaggaatc ggcggacatc
    22681 tacgacaagg gcggccggcc ggagcttgcc gccggcgagc gcgcggaaat cgcggtcatc
    22741 tcctcgttcc tgccgcagca gatggatgag gccgaggtca aggccgccat cgccgccgcc
    22801 attgccgcca ccggcgccgc ctcgatgaag gacatgggca aggtgatcgc cgaattgcgc
    22861 ggcaaattcg ccggccgcat ggatttcggc aaggcgagcg ggctggtcaa ggccgctttg
    22921 ggcgggtgag gggttcgagg tggatttccc accagatggg ctctgtgttg tcgtggtcga
    22981 tccgataatg tcgaaccagg gttttggcca gcgcgcgcgc gcgcttttct gatccgacga
    23041 ttatcctttg cagagccggc agtccggcga ggggggcggc gtcggtgatc tgcgtgttgc
    23101 tcaggtcaag cgtggtcagc gcggtcagtc cggcgagggg ggcggcgtcg gtgatctgcg
    23161 tg
//
LOCUS       DAOMXG010000018        23108 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_1367_length_23108_cov_9.716870, whole genome shotgun sequence.
ACCESSION   DAOMXG010000018 DAOMXG010000000
VERSION     DAOMXG010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 23108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23108
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1367_length_23108_cov_9.716870"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2523)
                     /gene="xdhB"
                     /locus_tag="PLQ11_02425"
     CDS             complement(<1..2523)
                     /gene="xdhB"
                     /locus_tag="PLQ11_02425"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdopterin binding
                     subunit"
                     /protein_id="HRK23787.1"
                     /translation="MSPVGKMAVVGQPLTHDSAALHVAGAAPYIDDLTEPTGTLHLAL
                     GLAPVAAGRITGLDLGAVRAAPGIVRVLTAADIPGRDECSPSIGGDPVLAADRAQFHG
                     QAVFAVVAETRAAARKAARLGHVMVEADDPVVTVAAALDADATVLPDYAFRRGGVAEA
                     LAAAPHRVTGAFAIGGQEHFYLEGQIALAIPGEDGAVVVHSSTQYPNEVQHIVAGVLG
                     VAMHKVTVICRRMGGGFGGKESQASQWAALAALAARLTGRACKLRLDRDDDFVLTGKR
                     HDFDVQYTTGFDNDGRIAGHDVLYCARCGCSADLSQGVVDRTMFHAANAYFLPATQIA
                     TRRLRTNTVSATAFRGFGGPQGMLAMERVIDAIAWRLGRDPLDVRKANFYRPGSDVTP
                     YGQTVEDCETLPALVEALEVSSGYWARRAEIAAFNRGSPILKRGLALTPVQFGISFTL
                     MHLNQAGALVHVFADGSVTLNHSGTEMGQGLNLKVAQVVAEAFGLPVGQIRITATQTD
                     KVPNGSATAASAGSDLNGMAALNAAQEIRARMAVVAAGQLGGRAEDVVFAGGQARCAG
                     REIGFADLARQCWLNRVSMSATGFYQTPDIHWNRAAGSGKPFLYFAYGAACSEVMIDS
                     LTGEMRVLRVDIRHDCGRSLNPAIDRGQIEGGFVQGMGWLTSEELVFGRDGRLLTHAP
                     STYKIPVASDVPADFRVSLHDSGRLPNTVFHSKAIGEPPLMLALSVFSAIGDAVHSLH
                     PGRSVALDAPATPEAIQRADGEGTFGGREDREHRGEDVVMVEPASHDVGDDVEAADEV
                     ELLEDHGAARTPFEQVLALQRRDVAALIEDLAGCGIVQPVDHA"
     gene            complement(2520..3989)
                     /gene="xdhA"
                     /locus_tag="PLQ11_02430"
     CDS             complement(2520..3989)
                     /gene="xdhA"
                     /locus_tag="PLQ11_02430"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008546184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase small subunit"
                     /protein_id="HRK23788.1"
                     /translation="MQRSVIRYLKRGEVISFAPNEPCMTLLDHIRLERRETGTKEGCN
                     EGDCGACTVVLARLEAGRLSYRPVNACILLAGQIDGAELITIEDLASGDRLHDVQQAM
                     VDHHASQCGFCTPGIVMSLFALYHGPRPVDRAAINSQLAGNLCRCTGYRPIVDAALDT
                     CAKLPDDRFSAGEAGRRAALATLDDGADVVIGDDRRFFAAPARLDTLADLYLRHPDAV
                     LIGGATDVGLWITKKLKEIDKIIWLGRIRDLDRIETGPDRLLLGSGVRLADAADALAD
                     LDPDLGGVMRRFGSAQVRASGTVGGNIANGSPIGDLAPCLIALGASVTLQRGGKQRCL
                     PLEQFFLAYGRQDRHPGEFVRMLTIPRLKADQQFRAFKISKRFDEDISAVMGAFRLDV
                     TEGVIGTARVAFGGMAATPKRAAETERRLAGLRLDSPDSWRVALDALAGDFSPISDMR
                     ATADYRRRTARALLEKALLEVAGAGSATTRLFERGEARR"
     gene            complement(4007..4993)
                     /gene="iolG"
                     /locus_tag="PLQ11_02435"
     CDS             complement(4007..4993)
                     /gene="iolG"
                     /locus_tag="PLQ11_02435"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HRK23789.1"
                     /translation="MLTIGLLGAGRIGRIHGGNIAGHPKARLVAVADASAAAAEALAS
                     ETGAKTASIEAILADRSIGAVVICTPTDMHADLIEAAAKAGKAVFCEKPVDLNAERIR
                     ACLRVVDKAGVPLMIGFNRRFDPNFASVRQRIAAGEAGKVELVTILSRDPNPPPAEYV
                     ARSGGLFRDMMIHDLDMARFLIGEEPVEVHAVGSSLVDPAIGKAGDVDTAAVLLKTAS
                     GKICQISCSRRATYGYDQRIEVHGSKGMIRAGNVHNTTVEVANGAGVHADPVQNFFLE
                     RYAAAYRIELASFIAAVESGARPSPSGDDGLKAQILADAATQACFSGGPVQL"
     gene            5141..5956
                     /locus_tag="PLQ11_02440"
     CDS             5141..5956
                     /locus_tag="PLQ11_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017968010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRK23790.1"
                     /translation="MSATTSPCFRLDGKVAIVTGGTQGVGEAIARELAARGLSDLVIC
                     GRQFDRGEAVARELSAQGCKTLFVQADLAVTAEAQAVVHATDRAFRRVDILVNAAGIT
                     DRGNIFDTTPELFDRMMAVNVRAPFFLIQETVKIMKREAITGSIINIQSMSAHGGQAF
                     IAAYCVSKGALATLTSNTANALLNHRIRVNGLNIGWMATPGEDRIMRLYHGASDGWLE
                     AAEKTRPFGRLIDPKEVARACAYLASGESGLMTGANIDFDQMVAGTSDPPLPV"
     gene            5966..7474
                     /gene="glpD"
                     /locus_tag="PLQ11_02445"
     CDS             5966..7474
                     /gene="glpD"
                     /locus_tag="PLQ11_02445"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HRK23791.1"
                     /translation="MTSYDLVVIGGGINGCGIARDAAGRGLSVALFEMRDLASGTSSA
                     STKLIHGGLRYLEYYKFRLVRESLLEREVLWKIAPHIIWPLRFVLPYQAGLRPMWLIR
                     LGLFLYDHIGGRKLLRATRTLDLTRSSAGKPLRPGMHYGFEYSDCWVDDSRLVVLTAL
                     SAAEHGARIATQARVVAAERRHGSWLLTVRHADGSEEAVGARMLVNAAGPWVADVLKT
                     VGPVTSAAGIRLVQGSHIIVPRLFDHSRAYIFQNADNRIVFAIPYQRDFTLIGTTDRD
                     WHDEPGKVAASAEEIAYLCAAASEYFLHAITPADVVATYSGVRPLYDDGASKAQEATR
                     DYVLHLDEAEDAPLLSVFGGKITTYRRLAETALDRLGAYLPAEAQARRGWTGTAPLPG
                     GDFAHDGYQHQVAALRRAYGFLPSALAQRLVRAYGTRASGLLGDSRSLADLGKHYGAG
                     LYEKEVAYLMQHEWAKTTEDIIWRRTKLGLVLSSDEIAQLNQSVSLRKTASI"
     gene            7630..9312
                     /gene="pgi"
                     /locus_tag="PLQ11_02450"
     CDS             7630..9312
                     /gene="pgi"
                     /locus_tag="PLQ11_02450"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HRK23792.1"
                     /translation="MAEALPFDTPAWRALRRHARSLRGVEMRSLFAGDADRFRRLSLK
                     AEGLLLDYSKNCLTTRTRALLVRLAEASDLAARRDAMFGGAAINTTERRSVLHTALRN
                     LAGTPVLVDGRDVMPEVAAERARCLAFAEAVRGGTIRAANGKPFTAVVNIGIGGSDLG
                     PAMVTRALSPFCHRRIASLFVSNVDGADLADTLARLDPATTLVIVSSKTFTTQETMMN
                     AVSARAWIAGALGEAAVGAHFAAVSTNMAATQAFGIDASRVFRFWDWVGGRYSVWSAI
                     GLPVMIAIGARNFEAFLGGAEAMDRHFRNAPFHRNMPVLMALTGVWHRSILGYPSHAV
                     LPYEQRLARFPAYLQQLDMESNGKGVRLDGSAVAGATGPIIWGEPGTNGQHAFYQLIH
                     QGTNVIPADVLIGAEPVAADASHHRALVANALAQTEALMRGRTLDEARAQLAAAGAAP
                     AEVERLAPHKVFSGNRPTNTLIYRRLDPATLGKLIALYEHKVFVQGVLWNINSFDQWG
                     VELGKELCARLMPVVAGERPPVGLDASTAGLVDHLLKVSKTLTRGKPGYTGL"
     gene            9392..11827
                     /locus_tag="PLQ11_02455"
     CDS             9392..11827
                     /locus_tag="PLQ11_02455"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HRK23793.1"
                     /translation="MPDAAEVASFKQAVEAKLNYAVGKDASVASERDWFLATTLAVRD
                     KIVDGWMESSRATRAQGRKRVYYLSMEFLIGRLLTDALNNLGVTDIVRSALAELNVDL
                     EVLKEVEPDAALGNGGLGRLAACFMESMATLSVAAYGYGIRYDHGFFRQQINNGWQEE
                     YPEDWLWFGNPWEFERPEAQYNIGFGGTVSQGAGDEPRAQVWKAAETIKAIAYDMPVV
                     GWRARHFNTLRLWRARTPEPLRLDAFNQGDYIAALANRVKAEAISKILYPSDATPAGQ
                     ELRLRQEYFFVSASLQDLVRRHISQNGDIRALGRMVAIQMNDTHPAIAVAELMRILVD
                     LHDVDWKDAWAITREAMAYTNHTLMPEALESWPVPLMERLLPRHMQIIYLINADHLES
                     LRKAGRSDPALLASVSLIDEHNGRRVRMGHLAFVGSHAVNGVSALHSDLMKKTVFHDL
                     HQVYPDRIQNKTNGIPFRRWLMEANPGLTRCLTETLGDRLLDDPAALSELVNHADNAS
                     LHARLERVRYANKVRLAKLIHDRIGVRVDPAAMFDVQIKRIHEYKRQLLNLLETVALY
                     NAIRANPSGDFVPRVKIFAGKAAPSYHLAKLIIKLANDIARIVNNDPTVRGLLKVAFL
                     PNYNVSLAEMIVPAADLSEQISTAGTEASGTGNMKLALNGALTIGTLDGANIEMRDHV
                     GAENFFIFGMKADEVEARRAAQGISGQPAIAASRWLGQVLDSIASGVFSPDDPGRYRE
                     LVEVLRNYDHYMVTADFDSYFATQRQIDACWTDKASWWSKSIHNTARMGWFSSDRTIR
                     EYAQDIWGASVSA"
     gene            12280..13548
                     /gene="glgC"
                     /locus_tag="PLQ11_02460"
     CDS             12280..13548
                     /gene="glgC"
                     /locus_tag="PLQ11_02460"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041805083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HRK23794.1"
                     /translation="MSVRVPASAPLSRYAMAYVLAGGRGSRLMELTDRRAKPAVYFGG
                     KSRIIDFALSNAVNSGIRRIAVATQYKAHSLIRHLQQGWNFFRTERNESFDILPASQR
                     VSEDRWYIGTADAVFQNLDIVQDYDPKFIVLLAGDHVYKMDYEMMLQQHVEQGADVTV
                     GCLEVSKKEATGFGVMQVDENDRIVEFYEKPKKPPTMPGRPDRCLASMGIYVFNTSLL
                     ADELNRDANDPNSSHDFGKDIIPYLVKHGKAVAHHFSRSCVRSESEKTAYWRDVGTVD
                     AYWAANIDLTDVVPELDLYDRSWPIWTYGEITPPAKFVHDEDGRRGHAVSSLVSGGCI
                     VSGSRLKRSLLFTGVRVNSYSSIENAVILPYVDVGRHARLRNVVVDRGVRIPEGLVVG
                     DDPVLDAHRFRRSDHGVCLITERMIAQLGK"
     gene            13552..15006
                     /gene="glgA"
                     /locus_tag="PLQ11_02465"
     CDS             13552..15006
                     /gene="glgA"
                     /locus_tag="PLQ11_02465"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HRK23795.1"
                     /translation="MAKIKVLSVTSEIFPLIKTGGLADVAGALPLALGRLDVEMRTLV
                     PCYPAIREKLKKAVLEHEFADFYGGKARLLSATFNDLPLLLLEAPHLYDRPGNPYVMA
                     DGQNWPDNAARFAALARAGASIGQGLLAGFKPDIVHAHDWQAGLVPAFLHYDARPAPK
                     TVMTVHNLAFQGQFGPEWLARIGLPPQAWHTDGVEYYGTIGYLKAGLRFADKITTVSP
                     TYAVEIRSPAAGMGLDGLINARAGDLVGIINGIDTRVWDPGSDDALESTFDSDHFGAR
                     WANRKALQERMGLDQDPDALLCGIVTRLSWQKGLDLLLEAIPALMRLGGQLVLLGAGD
                     ANLESGFRAAAATYPGRIACEFGYDERLAHQIQAGSDSILVPSRFEPCGLTQLCALRY
                     GAVPIVARVGGLADTVIDANEMALANGVATGIQFFPVAREPLIEALTRAARLFAVPDD
                     WQLMQTNGMNTDVSWSGPARRYADLYRSLLGRAA"
     gene            15026..16654
                     /locus_tag="PLQ11_02470"
     CDS             15026..16654
                     /locus_tag="PLQ11_02470"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HRK23796.1"
                     /translation="MIKTVATTPFSDQKPGTSGLRKKVTVFETPHYVENFIQSIFDSL
                     EGFAGKTLVVGGDGRYFNRQTIQTVIRMAAANGFGGVLVGRAGILSTPAASHLIRKSG
                     AFGGIILSASHNPGGPDGDFGIKYNAGNGGPAPEKITDAVFARSKSISAYRTVEAADV
                     DLDRIGATRIAGMSVEIVDPVADYAALMQTLFDFDRIRAMLNGGFRLTFDAMSAVTGP
                     YGHAIFETLLGAPKGSVLNGVSLPDFGHHHPDPNLVHCKHLYDLAHGPNAPDFCAASD
                     GDGDRNLIIGRGQFVTPSDSLAMIAANAHLAPGYAKGLAGVARSMPTSGASDRVAEKL
                     GINSYETPTGWKFFGNLLDAGLVTICGEESAGTGSNHVREKDGVWAILMWLNILAARR
                     QSVQAIVREHWATYGRNYYARHDFEEVASDGANALMQHLRTSLGTLKGQRFGGLDVAA
                     ADDFAYHDPVDGSDTSQQGVRVLFADGSRIVYRLSGTGTVGATLRVYIERYEPASGTL
                     GLDTGIALKELIALSRSLARITHFTDRTEPDVIT"
     gene            16654..21009
                     /gene="glgX"
                     /locus_tag="PLQ11_02475"
     CDS             16654..21009
                     /gene="glgX"
                     /locus_tag="PLQ11_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HRK23797.1"
                     /translation="MDHRRTAGSPEPLGVTSTATGINVAVFSAHATRIDLCLFDAAGT
                     REIARIPLPERTGDVWHGHIEGVSIGARYGLRAHGPYDPVVGHRFNPFKLLVDPYAVA
                     LDRRLVLHPDMFGYRPGEWDLSFSDIDSAPVMPKAIVTPPVAPAPRAPLHGWPETILY
                     ELNVRGFSRANSAVPEAMRGTFRGLGHPASIAHLASLGITTVEIMPAAAWIDERHLPP
                     LGLTNAWGYNPVAFMAPEPKLAPGGWADIRTAVDALHQAGIEVIVDVVLNHCGESDEL
                     GPTVSLRGLDNASYYRLNPHNLARYIDDMGCGNVLALDRPPVVRMAMDALRTWVRRAG
                     IDGFRFDLATALGRRADGFDPAAPLIAALRQDPELCGLKLIAEPWDIGPGGYQAGRFP
                     AGWGEWNDRYRDDVRRFWRGESFMLGALATRIAGSSDLFHTKRPARSINFVTAHDGFA
                     LADLVSFSVKHNDANGENNRDGTDANYSWNHGIEGPTADPAITEARRRDQINLLATLL
                     LSRGTPMLQMGTELGHSQAGNNNAYAQDNALTWLDWSAPDSQLLEAARALIAARRRFG
                     LIAEDRFLTGEPRELDGNPDAQWMTPAGQPLAPEDWGSPDAATLALHLTGAGADPARL
                     LMILHRGGEPVAHAVPATEPGRLWEVAVDTARPATEWRKPLPSGTNRIDIAPRSVVLL
                     AEAPDPAAGRKGPPAARPATVDRLASAVGITADWWDLGGTCHVVPEATRRALLTAMGL
                     DVTSQGGARDALEQLAETRDRRRLPATLHGRAGRPIAVPVVCGAAPDKPPRMLSLTDE
                     AGARIEIEWDADHSRRVSCLAADGRPFERIWSDLPPLAAGRYRLLDETTGLDCQLTVA
                     PERAQAPMAPGFGLSAQLYTLRSGADQGIGDFSTLAEAVRHAANAGAATFGINPLHAL
                     NGSDRSRVSPYHPSDRRFLDPIYIDVTALDDLPSSPLAEAMLAAAAPEFEGLRAARSI
                     DYARVSALKLAILKARFEAFRTADFGGEPLRSAFFAFVETGGAALERFATFRLIEERQ
                     AGKPWATWEGGLADANSRAVRNFAEQHRFALAFEMYLQWLADRQLAAAAQAARDAGLS
                     LGLYRDLAVGCAPDGAEVWAQARHWACGASVGAPPDPFSATGQIWNLPPPNPLTLHEG
                     GFSAWRSLLAANMRHAGMLRIDHVMGLTRLFCVPDGALAAEGAYVAYPRDALLAELQL
                     ESHRRGCRVVGEDLGTVPDGFRQAMADADILSYRVLWFEREGEDFRDPATYPADAVAC
                     LATHDLPTLAGWWNGSDLAEAAGLGRIDDPAAAAAARAAERQRLIDALIGKGLIPAAP
                     AKDAPLDAATAAALHAYVAASPCRIAFAQMDDLAGETEAVNLPGTDRERPNWRRRLET
                     GLDSLFADQAARSILAAMAIGRSDTISLRRAGELAKEQDHGSDPNAPDDPARPADGKS
                     GARRAGARSRAADAG"
     gene            20906..21916
                     /locus_tag="PLQ11_02480"
     CDS             20906..21916
                     /locus_tag="PLQ11_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRK23798.1"
                     /translation="MALTRTRLMTLLVPLTASLALAALALGPVRLTPAEVAAALFGQP
                     DDPVHAIIVHDIRLPRMVLAGLIGAVLGLSGAALQGLTRNPLADPSLFGAPQSAAFAA
                     VAMVGFGGVDILSAWMPVAGIIGALASVGLLLLVTGRGAPMLVVVLAGLAIASLAGAA
                     TALTMNLAPNFYAALEIAFWLMGSLEDRSLRHLWLAAPFILAGSALLLAQGRALRALT
                     LGEDVAASLGVAVSRTRVAVIVGVALGVGAAVAVSGAIGFIGLVAPHLVRPLVGHDPG
                     RLLGASAWAGAALLLAADCLVKLAPASTDLKVGVITALVGAPFFLSLILNQRRLFAGD
                     AQ"
     gene            21913..22683
                     /locus_tag="PLQ11_02485"
     CDS             21913..22683
                     /locus_tag="PLQ11_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRK23799.1"
                     /translation="MTGWLALQSVSVVLGNRTVVADVNATFAPGQFVAIVGPNGAGKT
                     SLLRAVAGRLPCSGSIMLAGQPLAAITLSERARLIGYLPQGHQTHWPLPVRDIVGLGR
                     LAHGAGDPRRLTGADAAAVADAITRTGIGAILDQPATDLSGGERARVALARVLAQQTP
                     LVLADEPTASLDPRYQLQIANLLRDLAREGVLVLAVTHDLSHAAHFADRVLVMNRSQV
                     VRDAAPSAALDDATLAEVFGVRAASDASAARVPWEVLP"
     gene            22680..>23108
                     /locus_tag="PLQ11_02490"
     CDS             22680..>23108
                     /locus_tag="PLQ11_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRK23800.1"
                     /translation="MRRLLATLALTVIALLPAAARPRVVSINMCTDQLLLALADPDQI
                     LGLSYLAREPSLSWYWREAVGFRSLSGTAEDVLPLRPDLVFSATILKAPTRTMLRATG
                     LRVEEFASPRTIAEAITQIERAGALLDQPARAAAAIARIEA"
BASE COUNT         3857 a         7950 c         7108 g         4193 t
ORIGIN      
        1 cgcatggtca accggctgaa cgatcccaca tccggcaaga tcctctatga gggccgcgac
       61 gtcacggcgc tgaagggcaa ggacctgctc gaatggcgtt cgcgctgcgc catggtcttc
      121 cagcagttca acctcgtcgg ccgcctcgac gtcatcacca acgtcatgac tggccggctc
      181 taccatcaca acatcttcac ctcgatgttc tcgatcttct cggccgccga acgtgccttc
      241 gccatccgcg cgctggatcg cctcgggcgt cgccggcgca tcaagcgcca ccgagcgccc
      301 gggatggagg ctgtgcaccg catcgccgat cgccgagaac accgacagcg ccagcatcag
      361 cggcggctcg ccgatcgcct tggaatgaaa caccgtattc ggcagacggc cgctgtcgtg
      421 caagctgacg cgaaaatccg ccggaacgtc ggacgccacc gggatcttgt aggtggacgg
      481 ggcatgtgtc agcagccgcc cgtcgcgacc gaaaacaagc tcttcgctgg tcaaccagcc
      541 cattccctgc acgaagccgc cctcgatctg cccccggtcg atggccggat tgagcgagcg
      601 gccgcaatcg tgccggatgt ccacgcgcag cacccgcatt tcgccggtca agctatcgat
      661 catcacctcg ctgcacgccg ctccataggc aaaatagaga aatggcttgc cactgcccgc
      721 cgcgcggttc cagtggatgt cgggtgtctg gtagaacccc gtcgcggaca tcgagacccg
      781 gttcagccag cattgccggg caaggtccgc aaatccgatt tcccggccgg cacatcgcgc
      841 ctgtcccccg gcgaaaacca cgtcctccgc gcgccccccg agctgaccgg cagcgaccac
      901 cgccatgcgc gcgcggatct cctgcgccgc gttcagtgcc gccattccgt tgaggtccga
      961 gccggccgag gcggctgtcg cagaaccgtt cggcaccttg tcggtctggg ttgccgtgat
     1021 ccggatctgc ccgaccggca ggccgaaggc ttcggccacc acctgcgcca ccttcaggtt
     1081 cagcccctgc cccatctccg tgccggaatg gttgagcgtc accgacccat cggcgaacac
     1141 atgcaccagc gcgcctgcct ggttgagatg catcagcgtg aaggaaatgc cgaactgcac
     1201 cggcgtcaac gcaagaccac gcttgagaat gggcgagccg cggttgaacg cggcgatctc
     1261 cgcccgcctt gcccaatatc cgctggatac ctccagcgcc tcgaccagcg ccggcaaggt
     1321 ctcgcaatcc tcgactgtct ggccataagg cgtgacatcg gagccggggc gatagaaatt
     1381 ggccttgcgc acatcgagcg ggtcacgtcc aaggcgccag gcgatggcat caatgacccg
     1441 ctccatcgcc agcatgccct gcggcccgcc gaacccccgg aacgctgtcg ccgagaccgt
     1501 gttcgtgcgc agccgccgcg tcgcgatctg cgtcgcgggc agaaaatagg cattggctgc
     1561 atggaacatc gtgcgatcga ccacgccttg cgacaggtcc gccgaacagc cgcagcgggc
     1621 gcaataaagc acgtcgtgcc cggcaatgcg tccgtcgttg tcaaatcccg tggtatactg
     1681 aacatcgaaa tcgtgccgct tgccggtcag cacgaaatca tcgtcgcgat cgagcctgag
     1741 cttgcaggcc cgccccgtca gccgcgccgc gagcgctgcc agcgccgccc attggctggc
     1801 ctggctctcc ttgccgccga aaccgccgcc catccgccgg cagatcaccg tcaccttgtg
     1861 catcgccacg ccgagcacac ctgcaacgat atgctgcacc tcgttgggat attgcgtcga
     1921 cgaatgcacg acaacagcgc catcctcgcc gggtatggcc agagcaatct gcccttcgag
     1981 gtagaaatgc tcctgcccgc cgattgcaaa ggcgccggtt acccgatgcg gcgccgcagc
     2041 cagcgcctcc gcaaccccgc cccggcggaa ggcataatcg ggcagcaccg tcgcatcagc
     2101 gtccagcgcc gcggcgaccg tcacaacagg atcgtccgcc tcgaccatga catgtccgag
     2161 tcgggcagcc ttgcgggcgg ccgctctcgt ttccgccacc accgcaaaca ccgcctgccc
     2221 atgaaactgt gcgcgatcgg ccgccaggac cggatcgcca ccgatcgacg gtgaacattc
     2281 gtcgcgcccc ggaatgtcgg cggccgtcag cacacggacg attcccggtg cggcccgcac
     2341 ggcgccaaga tcgaggccgg taatccggcc ggccgccacg ggagccagac cgagcgccag
     2401 atgcaatgtc ccggtcggtt cggtcagatc gtcgatatag ggtgctgctc cagccacatg
     2461 cagcgccgcg ctgtcatgcg tcagcggctg gccgacgacc gccatcttgc cgaccggact
     2521 catcgccgcg cctcgccccg ttcgaacaac cgggttgtcg ctgacccggc gccggcaacc
     2581 tccagcaggg ccttttccag cagggcccgt gccgtccgtc gacgataatc ggcagtcgcc
     2641 cgcatgtcgg aaatcggcga gaagtccccc gccagcgcat ccagtgctac acgccacgag
     2701 tcaggcgaat ccagcctgag cccggcaagg cgccgctccg tctctgccgc ccgctttggt
     2761 gtcgccgcca tgccgccgaa ggccacgcgg gccgtgccga tcacgccttc tgtgacatca
     2821 agacggaaag cacccatcac cgccgagata tcctcgtcga atcgcttgct gatcttgaac
     2881 gcgcgaaact gctggtcggc cttcagacgc gggatcgtca gcatacggac gaattcgccg
     2941 ggatgccggt cctgccggcc gtaggcgaga aagaactgtt ccagcggcag gcagcgctgt
     3001 ttgccgccgc gctgaagggt cacgctcgca ccaagcgcga tcagacaggg agccagatcg
     3061 ccgatcggcg acccgttggc gatattgcct ccgaccgttc cggaggcgcg cacttgagcc
     3121 gaaccgaacc ggcgcatcac ccccccaaga tccgggtcga gatcggcgag cgcgtctgcg
     3181 gcatcggcga gccttacccc gctccccagc agcaaccgat ccggaccggt ttcgatccgg
     3241 tcgaggtcgc ggatacgccc gagccagatg atcttgtcga tttccttcag cttcttggtg
     3301 atccacagcc cgacatccgt cgccccgccg atcagcaccg catccggatg ccgcagatag
     3361 agatcggcca gcgtatcgag acgggcgggc gcggcgaaga accgccggtc gtcgccgatg
     3421 accacatccg ccccgtcgtc aagcgttgcc agcgccgccc ttcgtccggc ctcgccggcg
     3481 ctgaaccggt cgtccggcag cttggcgcag gtatccagcg cagcatcgac aatcggccga
     3541 tatccggtgc agcggcagag attgccggcc agctggctgt tgatggcggc ccggtcgacc
     3601 ggtctcggcc cgtggtagag tgcgaacagg ctcatcacga tcccgggcgt gcagaagccg
     3661 cattgcgagg catggtgatc gaccatcgcc tgctgcacat catgcagccg gtcgcccgat
     3721 gccagatcct cgatcgtgat cagttcggcc ccgtctatct gcccggccag caggatgcag
     3781 gcattgaccg gacgatagct gaggcgcccg gcctccaggc gggccagaac caccgtgcag
     3841 gccccgcaat cgccctcgtt gcagccctcc ttggtgccgg tctcccgccg ctccagccgg
     3901 atatgatcaa gcagggtcat gcaaggctca ttgggagcga aggatatcac ctcaccccgc
     3961 ttgagatagc gtatgactga ccgctgcatc cgtttctctc gcgctgctac aattgcaccg
     4021 gacctcccga gaagcaggct tgcgtcgccg catcggcaag gatttgcgct ttcagaccat
     4081 catcgccgga ggggctgggc cgcgcgccgc tttcgaccgc cgcaatgaaa cttgcaagct
     4141 cgatccggta ggccgctgca tagcgctcga gaaagaaatt ctgcaccgga tcggcatgaa
     4201 cgcccgcgcc attggcgacc tcgaccgtgg tgttatggac attgccggca cggatcatcc
     4261 cttttgagcc atgcacctcg atgcgctggt cataaccata ggtcgcccgg cgtgagcagg
     4321 aaatctgaca gatctttccc gatgccgtct tgagcagcac cgccgccgta tcgacatccc
     4381 ccgccttgcc gatcgccgga tcgaccagcg acgagccgac cgcatgcacc tccaccggct
     4441 cttcgccgat cagaaaccgc gccatgtcga gatcgtggat catcatgtcg cggaacagcc
     4501 cgcccgagcg cgccacatat tcggctggcg gcggattggg atcgcgcgac aggatcgtca
     4561 ccagttccac tttgcctgcc tctcccgccg cgatccgctg gcgcaccgag gcaaaattgg
     4621 gatcgaaccg ccggttgaag ccgatcatca gcggcacgcc ggccttgtcc accaccctga
     4681 ggcaggcgcg gatgcgctcg gcattgagat cgaccggctt ttcgcagaac accgccttgc
     4741 cggccttcgc cgccgcctcg atgaggtcgg cgtgcatgtc ggtcggcgtg cagatcacca
     4801 ccgcgccgat gctgcgatcg gccaggattg cctcgatact ggcggttttc gcgccggttt
     4861 cgcttgccag cgcttcggca gcggcggcac tggcatcagc cacggccacc agccgcgcct
     4921 tgggatggcc cgcgatgttt cccccatgta tgcgcccgat gcgtccggcg cccaacagcc
     4981 caatggtcag cacgtctcat ccccgcgatg gtcaagttca tgaatcgagc gtcacaatgg
     5041 gacgtggcac cgcctcgcgc aagcaccgtt cgatcaacag cgcttgcgat agtggtgcag
     5101 cggcggcaga cttcacttcc gaccgcgaaa ggaatcgctc atgtccgcaa ccaccagccc
     5161 ttgtttccgc ctcgacggaa aagtggcgat cgtcaccggc ggcacccagg gcgtcggcga
     5221 ggcgatcgcc cgcgaactgg cggcgcgcgg cctgtccgac ctggtcatct gcggccgcca
     5281 gttcgaccgc ggcgaagccg ttgcccgcga attgtccgcg caaggctgca agacgctgtt
     5341 cgtgcaggcc gatcttgccg tcaccgcaga ggcgcaggcc gtggtgcatg ccaccgaccg
     5401 ggcattccgc cgcgtcgaca ttctcgtcaa tgccgccggc atcaccgatc gcggcaacat
     5461 tttcgacacc accccggaat tgttcgaccg gatgatggcg gtcaacgtcc gggcaccctt
     5521 cttcctgatc caggaaaccg tgaagatcat gaagcgcgag gcgatcaccg gctcgatcat
     5581 caatatccaa tcgatgtcgg cgcatggcgg gcaggcgttc atcgccgcct attgcgtctc
     5641 caagggcgcg ttggcaacgc tgaccagcaa caccgccaac gcgctgctga accaccgcat
     5701 tcgcgtcaac ggcctcaaca tcggctggat ggcgacgccg ggcgaggatc gcatcatgcg
     5761 gctctatcat ggcgcgagcg acgggtggct cgaagcggcg gaaaagaccc gtcccttcgg
     5821 tcgcctcatc gacccgaagg aagtcgcgcg tgcttgcgcc tatctggcat cgggcgaatc
     5881 cggtctgatg accggtgcga atatcgactt tgaccagatg gtggccggca cgtccgatcc
     5941 gccgctgccg gtctgagagg catccatgac cagttacgat cttgtcgtca tcggcggcgg
     6001 catcaacggc tgcggcatcg cccgcgatgc ggccgggcgc gggctctccg ttgccctttt
     6061 cgaaatgcgc gaccttgcct ccggcacatc atcggcctcg accaagctga tccacggcgg
     6121 cctgcgctat cttgaatatt acaagttccg gctggtgcgt gaatcgctgc tcgaacgcga
     6181 ggtcctgtgg aagatcgccc cacacatcat ctggccgctg cgctttgtcc tgccctatca
     6241 ggcggggctc aggccgatgt ggctgatccg gcttggattg ttcctttatg atcatatcgg
     6301 cgggcgcaaa ctcctgcgcg ccacccgcac gcttgacctg acccgttcct ccgctggcaa
     6361 gcccctgcgc cccggcatgc actacggctt tgaatattcc gattgctggg tggacgacag
     6421 ccggctggtg gtgctcaccg ccctgtctgc ggccgaacac ggtgcccgca tcgccaccca
     6481 agcccgcgtc gtcgccgcag agcgccggca cgggagctgg ctgctcaccg ttcgccacgc
     6541 cgatggaagc gaggaagcgg tcggtgcccg catgcttgtg aatgccgctg ggccatgggt
     6601 tgccgacgtg ctcaagaccg tcgggccggt cacatccgcc gccggcatcc ggctggtgca
     6661 gggcagccat atcatcgtgc ccaggctgtt tgaccattca cgcgcctata tcttccagaa
     6721 tgccgacaac cgcatcgtct ttgccattcc ctaccagcgc gatttcaccc tgatcggcac
     6781 aaccgatcgc gattggcacg acgaaccggg caaggtcgcc gccagcgcag aggaaattgc
     6841 ctatctctgt gcggcggcga gcgaatattt cctccatgcc atcacaccgg ccgatgtcgt
     6901 cgcaacctac tccggcgtcc gcccgctcta tgacgacggc gccagcaagg cgcaggaagc
     6961 gacgcgcgac tacgtgctgc atctcgacga ggcggaggac gccccgctgc tgtcggtgtt
     7021 cggcggcaag atcaccacct atcgccggct ggcggaaacg gcgctagacc gtcttggcgc
     7081 ctacctgccg gccgaggcgc aggccagacg cggctggacc ggcaccgcac ccctgcccgg
     7141 cggcgatttc gcccatgacg gctaccagca tcaggtcgcc gccttgcgcc gcgcctatgg
     7201 ctttctgccc agtgcattgg cgcaacgtct ggtgcgcgcc tatggcacgc gcgcatccgg
     7261 cctgctcggc gattcccgct ccctggccga tctcggcaag cattatggtg ctggccttta
     7321 tgaaaaggaa gtcgcctatc tgatgcagca tgaatgggcc aagactaccg aagatattat
     7381 ctggcgcaga acaaaacttg gactggtgct gagttccgac gaaatcgcac aactcaacca
     7441 atccgtaagc ttaagaaaaa ctgcttccat ctgagcggag cgagttacag attttagtaa
     7501 ttgcagccat gcaaaggtct tccctgcgac gatgggaacc ttgccgcctg atgcctcgat
     7561 catgccacga ggctcgttca gcgtcaggaa caaggcgatc aagccgccgc gaaaaggata
     7621 ctggtgagca tggctgaggc ccttccattc gacacgcccg cctggcgcgc tttgcggcgc
     7681 catgcccgct cgctccgcgg cgtggagatg cgcagcctgt ttgcgggtga tgcggatcgg
     7741 ttccgccgcc tgtcgctgaa ggccgagggc ctgctgctcg actattccaa gaactgcctg
     7801 accacccgca cgcgcgccct gctggtcaga ctggctgagg cgagcgatct tgccgcccgc
     7861 cgcgatgcca tgttcggcgg tgcggcgatc aacaccaccg agcgccgttc ggtgctgcat
     7921 accgccctgc gcaatctcgc cggaacgccg gtcctcgtcg atggccgcga tgtgatgccg
     7981 gaggttgcgg cagagcgggc ccgctgtctt gcctttgccg aggcggtgcg cggcggaaca
     8041 atccgcgccg ccaatggcaa gcccttcacc gctgtggtca atatcggcat cggcggctcc
     8101 gatctcggcc cggcgatggt gacccgggcg ctgtccccgt tctgccacag gcgcatcgcc
     8161 agcctgttcg tctccaatgt cgacggtgcc gatctggccg acacgctggc acggcttgat
     8221 ccggcaacga cgctggtgat cgtctcgtcc aagaccttca ccacccagga gaccatgatg
     8281 aatgccgtct cggcccgcgc ctggatcgcc ggcgcgctgg gcgaggctgc ggtgggcgcg
     8341 cattttgccg ccgtttccac caacatggcg gcgacgcaag ctttcgggat cgatgccagc
     8401 cgcgtgttcc gcttctggga ctgggtgggc ggccgctatt cggtctggtc ggccatcggc
     8461 cttcccgtga tgattgccat cggggcccgc aacttcgagg catttctcgg cggcgccgag
     8521 gccatggacc ggcatttccg caatgccccg ttccatcgca acatgccggt tctgatggcg
     8581 ttgaccggcg tctggcatcg aagcattctt ggctatccct cgcatgccgt cctgccgtac
     8641 gagcagcggc tggcgcggtt tccggcctat ctgcagcagc tcgacatgga atcgaacggc
     8701 aagggcgtgc gcctcgatgg cagcgcggtt gccggcgcca ccggcccgat tatctggggc
     8761 gaacccggga ccaatggcca gcatgccttc taccagttga tccatcaggg caccaatgtc
     8821 atcccggcag atgtgctgat cggcgccgag ccggtcgctg ccgatgccag ccatcaccgc
     8881 gcgctggtcg ccaatgctct ggcccagacc gaggccctga tgcgcggccg cacgctcgat
     8941 gaagcgcgcg cccagctcgc tgccgccgga gccgctccgg ccgaggtcga gcgtctcgcc
     9001 ccgcacaagg tgttctccgg caaccggccg accaacacgc ttatctaccg ccggctggat
     9061 ccggcgacgc tgggtaagct gattgcgctc tacgaacaca aggtgttcgt gcagggcgtt
     9121 ctgtggaaca tcaattcgtt cgaccaatgg ggtgtcgaac tgggcaagga actctgcgcc
     9181 cggctgatgc cggtcgtcgc cggggaacgt ccgccggtcg ggctcgatgc ctcgaccgcg
     9241 ggtctcgtgg accatcttct gaaggtctcg aaaacactaa caaggggaaa gcccggatac
     9301 accgggctct gaggggagtg cgttgcgtat gaaatctcat ctcaaggcca tgaagccggc
     9361 tgtcaatgac gatgccgtcc agcaccgtcc gatgcccgat gccgccgaag tcgcttcgtt
     9421 caagcaggcg gtcgaggcaa agctcaacta cgccgtcggc aaggatgcca gcgttgcctc
     9481 ggagcgggac tggtttctcg ccaccacgct ggcggttcgc gacaagatcg tcgacggctg
     9541 gatggaatcg agccgcgcaa cccgcgcgca aggccgcaag cgcgtctatt acctgtcgat
     9601 ggaatttctc atcggccgcc tgctgaccga tgccctcaac aacctcggcg tgaccgacat
     9661 cgtgcggtcg gccctggccg aactcaatgt cgatcttgaa gtgttgaagg aagtcgagcc
     9721 ggatgctgcg ctcggcaacg gtggcctcgg ccggcttgcc gcctgcttca tggaatcgat
     9781 ggcaaccttg tcggtggccg cctacggcta cggcattcgc tacgaccatg gttttttccg
     9841 ccagcagatc aacaacggct ggcaggagga atacccggag gactggctgt ggttcggcaa
     9901 tccctgggaa tttgaacgcc ccgaagcgca atacaacatc ggctttggcg gcaccgtcag
     9961 ccagggcgcg ggcgatgagc cgcgcgctca ggtctggaag gccgccgaaa ccatcaaggc
    10021 gattgcctac gacatgccgg tcgtcggctg gcgcgccagg catttcaaca ccttgcggct
    10081 gtggcgtgcc cgcacgccgg aaccgttgcg tctcgacgcc ttcaatcagg gcgactacat
    10141 cgcggcgctg gccaaccggg tcaaggccga ggcgatttcc aagatcctct atccgtccga
    10201 tgccacgccg gccgggcagg aactgcgcct gaggcaggaa tatttcttcg tctcggcctc
    10261 gttgcaggac ctggtgcgcc gccacatctc ccagaatggc gacattcgtg cgcttggccg
    10321 catggtggcg attcagatga acgatacgca tccggccatc gccgtggccg agctgatgcg
    10381 catcctcgtc gacctgcatg atgtggactg gaaggatgcc tgggcgatca cccgcgaggc
    10441 catggcctac accaatcaca cgctgatgcc cgaagctctg gaaagctggc cggtgccgct
    10501 gatggaacgg ctgctgccgc gccacatgca gatcatctat ctgatcaacg ccgatcatct
    10561 cgaaagcctg cgcaaggccg gtcgcagcga tcccgctctg ctcgcctcgg tctcgctgat
    10621 cgacgagcac aacggccgcc gtgtccgcat gggccatctg gcctttgtcg gctcgcatgc
    10681 cgtcaacggc gtgtccgcgc tgcattcgga cctgatgaaa aagaccgtgt tccacgatct
    10741 gcatcaggtc tatccggatc gtatccagaa caagaccaac ggcatcccct tccgccgctg
    10801 gctgatggaa gccaatccgg gtctgacccg ctgcctcacc gaaacactcg gcgatcgcct
    10861 gctggatgat ccggccgccc tgtccgaact ggtcaatcat gccgataacg ccagcctgca
    10921 tgcgcgcctc gagcgggtgc gctatgccaa caaggtccgg cttgccaagc tgatccatga
    10981 ccggatcggt gtgcgcgtcg acccggcggc gatgttcgat gtgcagatca agcgcatcca
    11041 tgaatacaag cgccagcttc tgaacctgct tgagacggtg gcgctctaca atgcgatccg
    11101 cgccaacccc tcgggtgatt tcgtgccgcg cgtcaagatc ttcgccggga aggcggcgcc
    11161 gagctatcat cttgccaagc tgatcatcaa gctggccaac gatatcgccc gtatcgtcaa
    11221 caacgatccg acggtgcgtg gcctgctcaa ggtggcgttc cttcccaact acaacgtctc
    11281 gcttgccgag atgatcgtgc cggccgccga cctctcggaa caaatttcca cggcgggcac
    11341 tgaagcctcc ggcaccggca acatgaagct cgccctcaac ggcgcgctga cgatcggcac
    11401 gctggatggc gccaatatcg agatgcgtga tcatgtcggg gcggagaatt tcttcatctt
    11461 cggcatgaag gccgacgagg tcgaggcccg ccgcgccgcc cagggcattt ccggccagcc
    11521 ggccattgcc gcctctcgct ggctcggcca ggtgctcgat tcgatcgcct cgggtgtatt
    11581 ctcgcccgac gatccgggcc gttaccgcga actcgtcgag gtgctgcgca actacgacca
    11641 ctacatggtc accgccgatt tcgacagcta cttcgccacc cagcgccaga tcgatgcctg
    11701 ctggaccgac aaggccagct ggtggtccaa gtcgatccat aacacggcga gaatgggctg
    11761 gttctcgtct gaccgcacca tccgcgaata tgcccaggac atctgggggg ccagcgtctc
    11821 cgcctgatct tggcagcacc gaaagacaca ttcatggccg gggcccgctc ggccatgagc
    11881 gtttttggac ctgcgctgaa cgcccccgtc tgtcatttgg ccgccggctg ccggcacttt
    11941 cgttggttct cgtcatggcc ggccgctggt caggagcgaa tgcgacgccg agcagggccg
    12001 aaggcccggt tgcgagggac ggcccgagaa atgaccggca ggtcatttgt gagggccact
    12061 ggtattatgg ccgcattgca ccggcattgg ccaaagagca acctttcata ccggaactgc
    12121 aaaacataca cgccctgcgt gccaatcttt gcaatgcggc aaaataggca ccgcgcgggt
    12181 ttactttttg cttttgtgtt cggcgtgaat ggctatgctt ccccaaggat cggcgctgct
    12241 agaggccacc ggcagcgtgg ttgtccgggg agatgcggaa tgtcggtacg ggtgccagca
    12301 agcgcgccgc tgtcgcggta tgcgatggcc tatgttctgg caggaggccg cggctcccgc
    12361 ctgatggagc tgaccgacag gcgcgccaag ccggcggtct acttcggcgg caagtcgcgt
    12421 atcattgatt ttgccctgtc caacgcggtc aattccggca tccgccgcat cgcggtggcg
    12481 acacaataca aggcgcacag cctgatccgc catctccagc agggttggaa cttcttccgc
    12541 accgagcgca acgagagctt tgacatcctg cctgcctcgc agcgcgtctc ggaggaccgc
    12601 tggtatatcg gcacggccga cgcagtgttc cagaaccttg atatcgttca ggattacgat
    12661 ccaaaattca tcgtcttgct ggctggcgac cacgtctaca agatggatta cgagatgatg
    12721 ctccagcagc atgtggagca gggtgcggat gtcacggtcg gatgcctgga agtttcgaag
    12781 aaggaagcga ccggcttcgg cgtgatgcag gtcgacgaaa acgaccgcat cgtcgagttc
    12841 tacgaaaagc ccaagaagcc cccgaccatg cccggccggc cggaccgttg cctggccagc
    12901 atgggaatct atgtattcaa cacgtcgctg ctcgccgatg aactcaatcg cgatgccaat
    12961 gatccgaatt ccagccatga tttcggcaag gacatcatcc cctacctggt caagcacggc
    13021 aaggcggttg cccatcactt ctcgcgctcc tgcgtgcggt ccgaatcgga aaagacggct
    13081 tattggcgcg atgtcggaac ggtcgatgcc tattgggctg ccaatatcga tctgaccgac
    13141 gtggtgccgg agcttgacct ctatgatcgc agctggccga tctggaccta cggcgaaatc
    13201 acgccccccg ccaagtttgt ccatgacgag gacggccggc gcggccatgc cgtctcctcg
    13261 ctggtctccg gcggctgcat cgtctccggc tcgcgtctca agcgctcgct gctgttcacc
    13321 ggtgtccggg tcaattccta ctccagcatc gagaatgccg tcattctgcc ctatgttgat
    13381 gtcggccggc atgcccgcct gcgaaatgtg gtggtggacc gcggggttcg tattcccgaa
    13441 gggcttgttg tgggtgatga cccggtgctc gatgcccacc ggttccgccg ctccgaccat
    13501 ggcgtctgcc tgatcaccga acgcatgatc gcccagctcg ggaaataggt gatggccaaa
    13561 atcaaggtcc tgtcggtcac ctcggagatt ttcccgctga tcaagaccgg cggcctcgcc
    13621 gatgtagctg gcgcgctgcc gctggcgctc ggccggctgg atgtcgagat gcgaacgctg
    13681 gtgccctgct atccggcgat ccgcgagaag ctcaagaagg cggttctgga acacgaattt
    13741 gctgatttct atggtggcaa ggcgcgcctg ctcagcgcca ccttcaacga cctgccgctg
    13801 ctgctgctcg aagcccccca cctctatgac cggcccggca atccctatgt catggcggat
    13861 gggcagaact ggccggacaa tgcggcgcgg tttgccgctt tggcccgggc cggcgccagc
    13921 atcggtcagg gcctgctggc tggtttcaag cccgacattg ttcatgccca tgactggcag
    13981 gccggtctgg ttccggcttt cctgcattat gatgcccgcc cggcgccgaa aacggtcatg
    14041 accgtgcaca atctggcgtt tcagggccag ttcggaccgg aatggctggc aaggatcggc
    14101 ctgccgccgc aagcctggca caccgacggc gtcgagtatt acggcacgat cggctatctg
    14161 aaggccggcc tgcgctttgc cgacaaaata acaaccgttt cgccgaccta cgcggtggaa
    14221 atccgttctc cggcagccgg aatgggcctt gacgggctga tcaacgcccg cgccggcgat
    14281 cttgtcggca tcatcaacgg catcgacacc cgggtctggg atcccggcag cgacgatgcg
    14341 ctcgaatcca cgttcgattc agaccatttc ggcgcccgct gggccaatcg caaggcgttg
    14401 caggaacgta tggggctcga tcaggacccc gacgctctgc tctgcggcat tgtcacccgc
    14461 ctgtcctggc agaaagggct cgacctgttg ctggaggcaa tcccggcgct gatgaggctc
    14521 ggcggccaat tggtgctgct cggcgccggc gatgccaatc tggaatccgg gttccgcgcg
    14581 gcggcggcaa cctatcctgg ccgcatcgcc tgcgaattcg gctatgacga gcgtctcgcc
    14641 catcagatcc aggccggcag cgactccatt ctcgtaccct cgcgttttga gccttgcggc
    14701 ctcacccagc tgtgcgccct gcgttacggc gcggtgccga ttgtcgcgcg tgtcggcggc
    14761 ctcgccgata ccgtgatcga cgccaacgag atggcgctcg ccaacggcgt tgccaccggc
    14821 atccagttct tccccgtggc gcgcgagccg ctgatcgagg ccctgacccg tgccgcccgc
    14881 ctgtttgccg tgccggacga ttggcaattg atgcagacca acggcatgaa caccgatgta
    14941 tcctggtccg gtccggcccg gcgctatgcg gatctttacc gttcgctgtt aggccgggca
    15001 gcgtgaagcc ggagagaccc ctcgcatgat caagactgtc gccaccacgc cgttttccga
    15061 ccagaagccg ggcacctcgg gcctgcgcaa gaaggtcacg gtgtttgaga ccccgcatta
    15121 tgtcgagaac ttcatccagt cgatcttcga cagtctcgaa ggctttgccg gcaagacgct
    15181 ggtggtgggc ggtgatggcc gttacttcaa tcgccagacc atccagacgg tgatcagaat
    15241 ggccgccgcc aacgggttcg gcggcgtgct ggtcggtcgc gctggcatac tttcgacgcc
    15301 ggccgcctcc catctcatcc gcaaatccgg cgcctttggt ggcatcatcc tgtctgccag
    15361 ccacaatccc ggcggcccgg acggcgattt cggcatcaaa tacaacgccg gcaacggcgg
    15421 ccccgcaccg gagaagatca ccgatgcggt gtttgcccgc tcgaaatcca tctctgccta
    15481 tcgcaccgtc gaggcggcgg atgtcgatct cgaccggatc ggcgcgaccc gcattgccgg
    15541 catgagcgtc gaaatcgtcg atccggtcgc cgattacgcc gcgctgatgc agaccctgtt
    15601 cgatttcgac cgcatccgcg ccatgctgaa cggcggcttc cgcctcacct tcgacgcgat
    15661 gagcgcggtg accggcccct acggccatgc catcttcgaa acactcctcg gagcaccgaa
    15721 gggcagcgtg ctcaacggcg tgtcgctgcc ggattttggc caccaccacc ccgatcccaa
    15781 cctcgtccat tgcaagcatc tctacgacct cgcccatggg ccgaatgccc ccgatttctg
    15841 cgccgcttcg gacggggacg gcgatcgcaa cctgatcatc gggcgggggc agttcgtcac
    15901 cccgtccgac agtctggcga tgatcgcagc caatgcgcat ctggcgccgg gctatgccaa
    15961 gggcctcgcc ggcgtggccc gttccatgcc cacctccggc gcctccgacc gggtggctga
    16021 gaagcttggg atcaattcct acgagacgcc gaccggctgg aaattcttcg gcaatctgct
    16081 tgatgccggg ctcgtcacca tctgcggcga ggagagcgcc ggcaccggtt cgaaccatgt
    16141 gcgcgagaag gatggcgtct gggccatcct gatgtggctg aacatcctcg ccgcgcgtcg
    16201 ccagagcgtt caggccatcg tccgcgagca ttgggccacg tacggtcgca actactacgc
    16261 ccgccatgat ttcgaggaag tcgccagcga cggcgccaat gcgctgatgc agcatctgcg
    16321 caccagcctc ggcactttga agggccagcg cttcggcgga cttgatgtcg cggcagcgga
    16381 tgactttgcc tatcacgatc cggtcgatgg ttccgatacg agccagcagg gcgtgcgcgt
    16441 gctgttcgcc gacggctcgc gcatcgtcta ccgcctgtcc ggcaccggca cggtgggcgc
    16501 gaccttgcgc gtctatatcg aacgctacga accggccagc ggaactctcg gtctcgacac
    16561 cggcattgca ttgaaggagc tgatcgcgct gtcgcgatcc ctggcccgca tcacccattt
    16621 caccgaccgc accgagccgg atgtgatcac ctgatggacc atcgccgcac cgccggatcg
    16681 ccggagccgc tgggcgtcac gtccacggcc accgggatca atgtggcggt gttctcggcc
    16741 catgccaccc gcatcgacct ctgcctgttc gatgccgccg gcacccgcga gatcgcgcgc
    16801 attccgctgc ccgaacgcac cggcgatgtc tggcatggcc atatcgaggg cgtgagcatc
    16861 ggcgcgcgtt acggcctgcg cgcccatggt ccctacgatc ctgtcgtcgg ccaccggttc
    16921 aacccgttca agctgctggt cgatccctac gcggtcgcgc tcgaccgccg cctcgtcctg
    16981 cacccggaca tgttcggcta ccggccgggc gaatgggatc tgtcgttttc cgacatcgac
    17041 agcgcgccgg tcatgccgaa agcgattgtc acgcccccgg tggcgccggc tccgcgcgca
    17101 cctttgcatg gctggcccga aaccattctc tacgaactca acgtgcgcgg attttcacgc
    17161 gccaacagcg ccgttccgga ggcgatgcgc ggcaccttcc gtggtcttgg ccatccggcc
    17221 tccatcgccc atcttgcatc gctcggcatt accacggtcg agatcatgcc cgccgccgcc
    17281 tggatcgacg agcggcacct gccaccgctc ggtctgacca atgcctgggg ttacaaccca
    17341 gtcgccttca tggcaccgga gcctaagctt gctcccggcg gctgggctga catccgcact
    17401 gcggttgacg ccctgcatca ggccggcatc gaggtcatcg tcgatgtcgt gctcaaccat
    17461 tgcggcgaga gcgacgaact cggcccgacg gtctcgctgc gcggcctcga caacgccagt
    17521 tactaccgcc tcaatccgca caatctcgcc cgttacattg acgacatggg ctgcggcaac
    17581 gtgctggcgc tcgaccggcc accggtagtg cgcatggcga tggatgcctt gcggacctgg
    17641 gtgcgccgcg ccggcatcga cgggttccgc tttgatcttg ccactgctct cggccgccgc
    17701 gccgatggct tcgaccccgc cgctccgctg attgccgccc tgcggcagga ccccgaactc
    17761 tgcggtctca agctgatcgc cgaaccctgg gatatcggtc ccggcggcta ccaggctggc
    17821 cggttccccg ccggctgggg cgaatggaac gaccgctacc gcgacgatgt ccgccgtttc
    17881 tggcgcggcg agagcttcat gctcggcgct ctggccaccc gcatcgccgg ctcatccgat
    17941 ctgttccaca ccaaacgtcc ggcgcgcagc atcaactttg tcaccgccca tgacggtttt
    18001 gcgctcgccg atctcgtcag cttcagcgtc aagcacaacg acgcgaatgg cgagaacaat
    18061 cgcgacggca cggacgccaa ctattcctgg aaccatggca tcgaggggcc gaccgcagat
    18121 cctgcgatca ccgaagcccg ccgccgcgac cagatcaacc tcctggcgac gctgctgctg
    18181 tcgcgcggca cgccgatgct gcagatgggc accgaactcg gccatagcca ggccggcaac
    18241 aacaatgcct acgcacagga caatgcgctg acctggctcg actggagcgc accggacagc
    18301 cagttgctgg aggcagcccg cgcgctgatc gccgcgcggc ggcggttcgg cctcattgcc
    18361 gaggaccggt tcctgaccgg cgagccgcgc gagcttgacg gcaatcccga tgcccagtgg
    18421 atgacgcccg ccggccagcc cctggcgccg gaggattggg gcagcccgga cgctgcaacc
    18481 ctcgccctgc acctgaccgg agcgggcgcc gatccggccc ggctgctgat gatcctccat
    18541 cgtggtggtg agccggtggc ccatgccgtc ccggcaaccg aaccggggcg gctgtgggag
    18601 gttgcggtcg acaccgcccg tccggcaaca gagtggcgca agcccctgcc atcgggcaca
    18661 aaccgcatcg acattgcgcc gcgctcggtt gtactgctgg cagaagcgcc cgacccagcc
    18721 gccggtcgca aaggcccgcc tgcggcccgc ccggccactg tcgaccggct tgcctccgcc
    18781 gtcggcatca ccgccgattg gtgggatctt ggcggcacct gccatgtcgt gccggaagca
    18841 acaaggcgag ccctgctcac ggcgatgggc ctcgacgtga cctcgcaggg aggagcccgc
    18901 gatgcactcg agcagcttgc cgaaacccgt gaccgccgcc gcctgcccgc gacgctgcat
    18961 ggccgcgccg gccggcccat cgcggtgccg gttgtttgcg gagcggcacc cgacaagcca
    19021 ccccgaatgc tgtcgctgac cgatgaggct ggcgcccgca tcgaaatcga atgggacgcc
    19081 gatcacagcc gccgcgtgtc ttgccttgcc gcggacggcc gtccgttcga gcgcatatgg
    19141 tccgatcttc cgccgcttgc tgccgggcgc taccggctgc tcgacgagac gaccgggctg
    19201 gactgccaac tgaccgtggc ccccgaacgt gcacaggcgc ctatggcacc cggattcggc
    19261 ctttccgccc agctttatac cctgcgctct ggtgccgatc agggaattgg cgacttttca
    19321 acccttgccg aggcggtccg ccatgccgca aacgccgggg cggccacgtt cggcatcaat
    19381 cccctgcatg cgctgaacgg cagcgatcgt agccgggtca gcccctatca cccctccgac
    19441 cgtcgctttc tcgacccgat ctatatcgat gtcacggcgc ttgacgatct gcccagttcg
    19501 ccgctggccg aggcaatgct ggccgccgca gcgcccgagt tcgaaggatt gcgggcagcc
    19561 agatcgattg attacgcgcg cgtctccgcg ctgaaactcg ccattctgaa agcccggttt
    19621 gaagctttcc gcaccgccga ttttggcggc gaacccttgc ggtccgcgtt tttcgccttt
    19681 gtcgagaccg gcggcgcggc gctggaacgg ttcgcgacat tccgcctgat cgaggagcga
    19741 caggcgggca agccctgggc cacctgggag ggcgggctgg ccgatgccaa ttcccgcgcc
    19801 gtgcgcaact ttgccgaaca gcaccgcttc gccctcgcgt tcgagatgta tctgcaatgg
    19861 ctggccgacc gccagctggc cgccgccgca caggccgccc gtgacgccgg tctcagcctc
    19921 ggcctctatc gtgaccttgc ggtcggttgt gcacccgacg gcgccgaggt ctgggcacag
    19981 gcccggcact gggcctgtgg tgcctcggta ggggcgccgc ctgatccttt ttccgccacc
    20041 ggccagatct ggaacctgcc gcctcccaat ccattgactc tgcacgaggg gggcttttcc
    20101 gcctggcggt cgctgctcgc tgccaacatg cgccatgccg gcatgctgcg catcgaccat
    20161 gtgatgggcc tgacgcgatt gttctgcgta cccgatggcg ccctggccgc cgaaggagcc
    20221 tatgtcgcct atccgcgcga cgcgctgctg gccgagttgc agcttgagag ccatcgccgt
    20281 ggttgccggg tggtcggtga ggatctcggc acggtgcccg atgggttccg tcaagcgatg
    20341 gcggacgcgg acatcctgag ctatcgggtg ttgtggttcg aacgcgaggg tgaggatttc
    20401 cgcgatcctg cgacctatcc cgccgatgcg gtcgcctgcc ttgccaccca cgatctgccg
    20461 acgctcgccg gctggtggaa cggctccgat cttgccgagg cggccggcct tggccggatt
    20521 gatgatccgg ctgcggccgc cgcagcccgc gccgcggaaa ggcagcggct gatcgacgcc
    20581 cttatcggca aggggttgat ccccgccgcc ccggccaagg acgccccgct cgatgctgcg
    20641 acagccgcag cgctgcatgc ctatgtcgcg gcgagtccct gccgcatcgc ctttgcccag
    20701 atggacgatc tcgccggcga gaccgaggcc gtcaacctgc ccggcaccga ccgcgagcgc
    20761 cccaactggc ggcggcggct cgaaaccgga ctggactcac tctttgccga ccaggcggcc
    20821 cgctccatcc ttgcggcgat ggccatcggt cgatccgata caatttcctt gcgccgggcc
    20881 ggcgagttgg cgaaggaaca ggaccatggc tctgacccga acgcgcctga tgaccctgct
    20941 cgtcccgctg acggcaagtc tggcgctcgc cgcgctggcg ctcggtcccg tgcggctgac
    21001 gccggctgag gtggcagcgg cgctgttcgg tcagcctgac gacccggttc atgcgatcat
    21061 tgtccacgac attcgcctgc cacgcatggt gcttgccgga ttgatcgggg cggttcttgg
    21121 cctttccggc gcggccttgc agggattgac ccgcaatcct ctcgccgacc cgtccctgtt
    21181 cggagcacct cagtccgcag ccttcgctgc cgtagccatg gttggttttg gcggggtcga
    21241 cattctgtcg gcctggatgc cggtagccgg catcatcggc gctctggctt cagttggcct
    21301 gctgctgctg gtgacaggaa gaggcgcccc gatgctggtt gtggtgctgg ccgggttggc
    21361 gattgccagt ctcgccggcg cggcaacggc cctgaccatg aatctggccc ccaatttcta
    21421 cgcagccctg gagatcgcct tctggctgat ggggtcgctg gaggatcgca gcctgcgcca
    21481 tctgtggctc gctgctccat tcattctggc tggcagcgca ttgctgctgg cacaggggcg
    21541 ggccttgcgc gcgctcaccc ttggcgaaga tgttgccgcc agccttggtg ttgccgtttc
    21601 ccgcacccgg gtcgcggtga ttgtcggcgt cgcgctcggc gtcggcgccg ccgttgcggt
    21661 ttcgggcgcc atcggtttca tcgggctggt ggcgccgcat cttgtccgtc cactggtcgg
    21721 ccacgatccc ggacgcctgc ttggcgccag tgcctgggcc ggagcggcat tgctgctcgc
    21781 ggccgattgt ctggtcaaat tggccccggc atcgaccgat ctcaaggtcg gtgtcattac
    21841 cgccctggtc ggtgccccgt tcttcctcag cctgatcctc aaccagcgac gcctgtttgc
    21901 cggagacgcg caatgaccgg ctggctggcg ctgcaatcgg tcagcgtggt ccttggcaac
    21961 cggaccgttg tggccgatgt caacgccacc ttcgcaccgg gccagtttgt cgccatcgtc
    22021 ggccccaatg gtgccggcaa gacaagcctg ctgcgtgctg ttgccggccg cctgccctgt
    22081 tccggcagca tcatgctggc gggccagccg ctcgccgcga taaccctgtc cgaacgcgcc
    22141 cggctgatcg gctatctgcc tcagggccat cagacacatt ggccgctgcc cgttcgcgat
    22201 atcgttggcc tcggccggct ggcgcatggc gccggcgatc cgcgccgact gaccggcgct
    22261 gatgccgcag ctgtcgccga tgccatcacc cggaccggca ttggcgccat tctcgaccag
    22321 ccggcgaccg atctttcggg cggcgagcgg gcccgtgtcg cgctcgcccg cgtgttggcc
    22381 cagcagacgc cgctggttct cgccgacgaa ccgaccgcct cgctcgatcc gcgctatcag
    22441 ttgcagatcg ccaatctgct gcgcgatctg gcccgggagg gggtcttggt gctggccgta
    22501 acccacgatc tcagccatgc ggcccatttc gctgaccgtg tcctggtgat gaaccggagc
    22561 caggttgtcc gcgacgccgc gccatcggcc gctctggacg atgcgaccct ggccgaagtg
    22621 ttcggggttc gcgcggccag cgatgcgtca gccgcgcgcg tgccctggga agtgctgccg
    22681 tgaggaggct gctggccacg ttggcgctga ccgtcatcgc cctgctgccg gcggctgcca
    22741 ggccacgcgt tgtctccatc aacatgtgca ccgatcagct gctgcttgcg ctggccgatc
    22801 ccgatcagat ccttggcctg agctatctgg cgcgagaacc cagtctatcc tggtactggc
    22861 gcgaggctgt tggctttcgc agcctgtccg gcaccgcgga ggatgttctg ccgctccggc
    22921 ctgatctggt tttttccgca accatcctca aggcaccgac ccgcaccatg ctgcgcgcca
    22981 ccggattgcg ggtggaggag ttcgcatctc cgcgcaccat tgccgaggcc atcacccaga
    23041 tcgaacgggc cggtgcttta ctggatcagc cagcgcgggc cgcagccgcc attgcccgca
    23101 tcgaggct
//
LOCUS       DAOMXG010000019        22639 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_1397_length_22639_cov_10.617207, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000019 DAOMXG010000000
VERSION     DAOMXG010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 22639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22639
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1397_length_22639_cov_10.617207"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1101)
                     /locus_tag="PLQ11_02495"
     CDS             complement(<1..1101)
                     /locus_tag="PLQ11_02495"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HRK23801.1"
                     /translation="MMGRILGLAVLAAAMVMPAALRAAPELVVDVSSGQVIHAEEATR
                     PWFPASTTKMMTAYVALRAMKAGQISLDTPLIASARAAGQPPSKIGLRPGQEITLDNA
                     LKVLMVKSANDVAVVIAEGVAGSLDGFSRLMNEEAARLGMRHSHFVNPHGLHSASHVS
                     SARDLAILGRALLVEFPQFRDYWGIGAVKLGARVMRNTNGLIGRYPGAEGMKTGFVCA
                     SGFNVVALANRGGRTMLAVVLGSGSGAERTIRTAQLLDKGFSSWGGGYGALTGLAESG
                     YGSAPNMRQEICRRGRQIFLGEEEDSGGAIMMPTMDSGSVFAVMMPQATAARSVGSIG
                     SGGRPSLGPRADLIPIEVTLGRTPGSTEVARGP"
     gene            1340..2506
                     /locus_tag="PLQ11_02500"
     CDS             1340..2506
                     /locus_tag="PLQ11_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HRK23802.1"
                     /translation="MTACIVGWSHTPFGKMDAETVESLIVRATREALTHAGISAGDVD
                     EIVLGHFNAGFSQQDFTAPLVLQADPAFRFKPALRTENACATGSAAIHQAIKSIEAKA
                     ARIVLVVGVEQMTRTPGPEIGKNLLRASYLPEDGETPNGFAGVFGNIAGAYFQKYGDN
                     SDALAAIAAKNHRNGVANPYAQMRKDVGFDFCRNESDKNPYVAGPLKRTDCSLVSDGA
                     AAVILADVETALAMRRAVAFRSRAHAQDFMPMSKRDILDFDGCRVAWGKALERARLRL
                     EDLSFVETHDCFTIAELIEYEAMGLTGKGEGKRAILEGWTEKTGRLPVNVSGGLKAKG
                     HPIGATGVSMHAITAMQLCGDAGDMQLTNPRLGGIFNMGGAAVANYVSILERIR"
     gene            2543..3748
                     /locus_tag="PLQ11_02505"
     CDS             2543..3748
                     /locus_tag="PLQ11_02505"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRK23803.1"
                     /translation="MLVLVVFLIAYIFSQFYRSCLAVIAADLARDLGLGPAELGHLTA
                     IWFITFSLAQFPIGYLLDRHGPRRTAALIMLVAVAGAALFSQATSFAMALVGMGLVGI
                     GCAPILMAALYYFGRTTPPERFPFYVSILVGVGNLGNILGARPLAQSVALIGWRSSLL
                     LIAVATLAAACAVFFTVRDPERVAAPNPGGNALSELWQVISFRTIWPLYLLTFVSYAT
                     IAAERGLWIGPFLEKVHGLDALARGDAAFFMALGMAMGALTAGPLVNVLGTMKRVAIM
                     GTSGAILGFGTLALVPGLGATLTVAILFLTGLFGMCYGIILSHARLFFPAHLLGRGVT
                     FVNFLSIGGAGVALSLSGLFVRAAELRGEGHAAIFSSLHMIFASALVIALAYYLTAPA
                     TPRDTHKTM"
     gene            4141..4935
                     /locus_tag="PLQ11_02510"
     CDS             4141..4935
                     /locus_tag="PLQ11_02510"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010684691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HRK23804.1"
                     /translation="MDFLKPRYTPMNRRRRIYEGKAKVLYEGPEPGTLIQHFKDDATA
                     FNAKKHEVIDGKGVLNNRISEFVFQHLNAIGVPTHFIRRLNMREQLIREVEIIPLEVV
                     VRNVAAGSLSQRLGIEEGTNLPRSIIEFYYKNDALNDPMVSEEHITAFGWATPQEIDD
                     IMALAIRVNDFLSGLFLGVGIRLVDFKMECGRLWEGDMMRIVVADEISPDSCRLWDIK
                     SNDKMDKDRFRRDMGGLVEAYTEVARRLGIIAENERINGTGPKLVQ"
     gene            complement(5112..6797)
                     /locus_tag="PLQ11_02515"
     CDS             complement(5112..6797)
                     /locus_tag="PLQ11_02515"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006725690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HRK23805.1"
                     /translation="MTTDIEIARAASLKPIHAVAAGLGIPADALSPYGHHVGKVALSH
                     LAGLSERPDGRLILVTAISPTPAGEGKTTTTVGLGDAFNRIGKKAIICLREPSLGPCF
                     GVKGGAAGGGHAQVMPMEQINLHFTGDFHAISAAHNLLAAMVDNHIYWGNGLGFDTRR
                     IAWRRAIDMNDRSLRAIVSSLGGAANGYPREDGFDITVASEVMAVFCLARDLEDLEQR
                     LGAMIVGYSRDKRPILARDLKAAGAMTVLLKDALAPNLVQTLEGNPAFVHGGPFANIA
                     HGCNSVIATRAGLKLGDYVVTEAGFGADLGAEKFFNIKCRKAGLVPSAAVLVATVRAL
                     KMHGGLARDELGREDLAALATGLANLRRHVENIRSFGVPSVVAINAFGSDSQAEHQAI
                     QSYCRNELGVEAVVCRHWAEGSAGTEELAIKVAQLAEAPQALDGRRFRPLYPAEMPLW
                     DKMRTIATRLYGASDIIADQKVRSEFKRLQAAGYGDLPICVAKTQYSFSTDPSLRGAP
                     VDHVVPVREVSLSAGAGFVVAICGDIMRMPGLPKSPSAEQIGLNAAGEVVGLF"
     gene            6987..8675
                     /locus_tag="PLQ11_02520"
     CDS             6987..8675
                     /locus_tag="PLQ11_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018688339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HRK23806.1"
                     /translation="MDDSTLERPKAGKGRRWQTRGRIADPAAARDIDLVLGHEPVRRD
                     HLIEYLHRIQDRFGALMPRHLKALADRMRLSEVEVFEVASFYHHFDIRRDDDAGPAAI
                     TIRVCDSVSCMLAGGEALVERLTDLVDPAHVRIMRAPCIGRCAEAPAALIGKRAVGHA
                     TAERLRDLAAAGTVTPEPVAATRLAEYQAAGGYRLLASMRSGALTPAAITEALSDAGL
                     RGLGGAGFPAGRKWSIVRSFPAPRLMTINADEGEPGTFKDRCLLEADPHRVLEGALIA
                     AQAVEAERIYLYLRDEYAHLHQLLGAEIDALARAELIKPGFIELRRGAGAYVCGEESA
                     MLASIEGRRGLPRHRPPYIAEAGLFGRPTLNHNVETLYWIRDILDHGAEWFAGQGLNG
                     ARGLRSFSVSGRVRFPGLKRAPAGITLRELVDQHCGGMAEGHRLAGYLPGGASGGMLP
                     ASRADLPLEFGRLEPHGCFIGSHAIVVFSDQDDPAAIALNLMQFFRHESCGQCTPCRL
                     GTEKLVALMEQGALDRALVADIDHAMREGSICGLGQAAPNPLACLIRYFPEALR"
     gene            8672..11488
                     /locus_tag="PLQ11_02525"
     CDS             8672..11488
                     /locus_tag="PLQ11_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008192275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRK23807.1"
                     /translation="MSDPIRFSIDGRPVDAEPGETLWQVAKRLGIAIPHLCHLDQPGY
                     RPDGNCRACLCEIEGERTLAASCIRKPAPGMVVHTASERVSRAQRTVFELIEAGLPAR
                     AGAPDPDAPFWHWSEQVAAPQGRLPGRHGVVSPEAAGLSLHDSSHAAIAVDLTACIAC
                     GLCERACREVQVNDVIGMAYRGIQTRPVFDADDPMGASTCVGCGECVQVCPTGALLEK
                     TVQTSIKQSGRLPIDREVDTLCPYCGVGCLTRVAVANDRIVRVDGRPGRANDSRLCIK
                     GRFGFDYVHHPHRLIRPLIRRDDAPKRGDASLDPSNPWPTFREASWEEALERAAAGFR
                     RILDRHGGAALAGFGSAKCSNEEAYLFQKLIRQGFGTNNVDHCTRLCHASSVTALMEG
                     IGSGAVSAPFNAALDADFIMIIGARPTENHPVAATYVKEAVRTGKTLVVADPRGSALM
                     RHATHALRFRPGTDVALLNAMLHVIVAENLYDPDYIAAHVDNFDALKANVAECSPEVM
                     APLCGIDPGMIRTVARLYARSRASLIFWGMGVSQHVHGTDNARCLIALALITGQIGRP
                     GTGLHPLRGQNNVQGASDAGLIPMFYPNYGPVDSPDLTARFQQAWGRPLDPARGLTVV
                     EILHAILADRIKGMLIMGENPAMSDPDQIHARAALARLEHLVVQDIFLTETAWHADVV
                     LPASAHAEKLGTFTNTNRQVQLGRPVLPLPGEARLDIDLLVSLANRLGLGWSYGQQPH
                     PEGGETGVEVSAVYEEMRGHMVSLKGLPWSRIAAEGSVTYPAADATSAGREVLFGDGF
                     PRAGGRARLVPARVSPPNEAPDERYPLVLTTGRLLEHWHTGSMSRRASVLAGLEPEGL
                     AAMHPDEISRRGLIAGDRVRIATRRGAIEARLRADRDVAPGMVFVPFAFNEAAANVLT
                     NPALDPFGKIPELKFAAAQVTALG"
     gene            11481..11711
                     /locus_tag="PLQ11_02530"
     CDS             11481..11711
                     /locus_tag="PLQ11_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012452776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HRK23808.1"
                     /translation="MAETAPKEASSPCIKLCTIDPGTGLCAGCGRTLEEIAAWSMMTD
                     AERIALNAALPDRLARRRRPVACRLPTSPSRT"
     gene            11771..12841
                     /gene="prfA"
                     /locus_tag="PLQ11_02535"
     CDS             11771..12841
                     /gene="prfA"
                     /locus_tag="PLQ11_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HRK23809.1"
                     /translation="MLPQDKLDAILARYDEINAHLAETQDSQLIIRLSKELAEIEALV
                     GKIRAWRGFAAAEAELLQLAEDPEMRALAEDDLARLRASHIEAERDLRLALIPRDAAD
                     EKGAILEVRAGTGGDEAALFAGDLFRMYQRYADFKGWRVEVISLSEGTMGGYKEIIAE
                     IQGVGVYAHLKFESGVHRVQRIPVTEAGGRIHTSAATVAVLPEAADVDMDIDDSDLKI
                     DTMRSGGAGGQHVNKTESAVRITHIPTGLAVVVQEERSQHKNKAKALAILRARLYDRE
                     RERLASERAADRRAQVGSGDRSERIRTYNFPQGRLTDHRIGLTLYKLEQIMEGSAFDE
                     VAQALITEHQAALLAGEDRGHG"
     gene            12834..13712
                     /gene="prmC"
                     /locus_tag="PLQ11_02540"
     CDS             12834..13712
                     /gene="prmC"
                     /locus_tag="PLQ11_02540"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HRK23810.1"
                     /translation="MAEALLTATTPYSEALGQMTTVLEAAGLAEARREARILLCAVLG
                     VGAEILILAPDAPLGSAAPAVQDALRRRSAREPLARITGHREFFGLDMELARSALIPR
                     PDTETLVEAVLDELGQSGRDQDALSLLDLGTGSGAILTALLSRLPQAGGVGVDRTIDI
                     LPHAARNCARHCGTGRALFIAGNWTDALGGTFDVIVSNPPYIATADIAGLDPEVRDHD
                     PRLALDGGSDGYAAYRALARAIPPRLAPGGLVGLELGAGQASTVRAIFAAAGLGSLSC
                     KRDLGGHERALLLRRA"
     gene            complement(13994..14380)
                     /locus_tag="PLQ11_02545"
     CDS             complement(13994..14380)
                     /locus_tag="PLQ11_02545"
                     /inference="COORDINATES: protein motif:HMM:NF015754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCCL domain-containing protein"
                     /protein_id="HRK23811.1"
                     /translation="MRVVQELVAYFDTRGQLSKRRLRTLLDKSRIAADGPTNMHGLCE
                     TVGATFYFRITGVAEGQVWGTDTYTRDSILGAAAVHSGLLKVGETAVLRVKVVPPPDS
                     YLGTTRNGVTTSDYGTFPHAWTLSAI"
     gene            complement(14377..15630)
                     /locus_tag="PLQ11_02550"
     CDS             complement(14377..15630)
                     /locus_tag="PLQ11_02550"
                     /inference="COORDINATES: protein motif:HMM:NF024601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA-like domain-containing protein"
                     /protein_id="HRK23812.1"
                     /translation="MSRPAQTADVERASLQRLTRGLRMVTVPFPHLTGLAAAVRVAVD
                     DSVPTMGVFASGRLVANSRFTARLSNDDLVFVLAHELLHLALRTHDRAVGSGRLEFNY
                     AHDYIINDMLRHALGLGVIPAGGLDMAGARERSAEEIVLEMRRNQSNSSRSRVWEGQI
                     VSIEQVLNSARQGQAGDMGDVLDADRERAMFPDDVADQMERARAIRDLAAKGMALGKA
                     LGLMKGRGTEAGGSQRQVRALRGIYRTPWQVALQRWLEGVSPGERTFTRASRRGADRA
                     DLVLPGRQRYSMMLHVVLDTSGSMSDEIPYALGAIADFCEATGVDEIRVIQCDTVVTG
                     DAMLSATELAEYEVRGYGGSDLSPALHQLAEDPRVTAAIVITDGDITYPREPMPYAVL
                     WAVPGDSTSFAPTYGRVITMSRGSS"
     gene            complement(15627..16661)
                     /locus_tag="PLQ11_02555"
     CDS             complement(15627..16661)
                     /locus_tag="PLQ11_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23813.1"
                     /translation="MTTGQAAFNEVVTLRQAKTLIRCMAHEQSFLLLSAPGIGKSDMV
                     YEAAREAGLPCRSLLGTQIAPEDVSGVPRIVGERSVFCPPRILLPERPEPFCLFLDEL
                     PACAPDVQKAFYSLLLERRLGEHALPPGTWVVAAGNRLQDRALVRAMSSALVNRVTIL
                     QIRCDTDEWLAWAQRSGIRQDIRSFITTVPDALMRAVPADPVPFSTPRAWSLLSRALD
                     LAEKAGLLTNDTRRALAFGRLSPEDAAVFCSLAEDNIGPVQPLARYIHEPDLLPRGDS
                     ARWFILDCLRQFVRDGRADGFAPRVINRFLGSLSTEHQLIVLTDMVEKWGALGAEKAM
                     LALLRKVAAA"
     gene            16937..19024
                     /locus_tag="PLQ11_02560"
     CDS             16937..19024
                     /locus_tag="PLQ11_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRK23814.1"
                     /translation="MPENTNAGRIAGPRLIALVGPFQSGKTTLLENLLVRSGALAREG
                     SIKDGSSVGDSSPEARAHSMSVEPNIASADYLGDRFTFVDCPGSIEFLHDMRNVLPLC
                     DAAIVVCEANPSKVPALQLILRELEDLGIPRLLFLNKIDTATQRVRETLQMLQPASRV
                     PLLLRQIPIWHNGIATGFIDLALERAFLYREHAPSVVIDVPAGEVPREKEARYSMLER
                     LADYDDSLMEELLTDIEPPRDQVFDDLARELREGLVVPVFIGSAERGNGVTRLLKALR
                     HESASIAATRARLGIGDSGPALANSIRSVHTAHGGKLTLARVLRGSFVENTKVLSAGG
                     HEDRIGSVSRMIGTAATRLPAAQEGDTLAFGRLDHVATGETFGDVKGPAPRPAVARAE
                     PPQPVHGMVLTIRDHKDEVKLAAAMTKLTDEDPALHFVQDQTSGETRLMGQGEMHLRV
                     ALERLTKRFGVAVDTRRASVAYRETIRHQVSVRGRHKKQSGGHGQFGDVALTVRPLER
                     GAGFQFAETVHGGTVPRQYFSAIEAGCRDVCEEGPLGFPVVDVHVTLTEGSYHTVDSS
                     DMAFRMAARLGMHEALKTAGPVLLEPILSVKIHVPSEALSRATGIVTGRRGQILGYDA
                     RAGWAGWDELNALMPEAEMTNLIVDLRSASAGVAGYTSSFDHLAEISGKAAETIIAAR
                     AHAKAASGHGHGA"
     gene            complement(19067..20908)
                     /gene="ilvD"
                     /locus_tag="PLQ11_02565"
     CDS             complement(19067..20908)
                     /gene="ilvD"
                     /locus_tag="PLQ11_02565"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRK23815.1"
                     /translation="MPAYRSRTSTHGRNMAGARGLWRATGMKDGDFGKPIIAIANSFT
                     QFVPGHVHLHDLGQLVAREVEKAGGVAKEFNTIAVDDGIAMGHDGMLYSLPSREIIAD
                     SVEYMVNAHCADALVCISNCDKITPGMLMAALRLNIPTVFVSGGPMEAGKFVAEGVTK
                     KVDLIDAMIAAADSSVSDAAVSAYERSACPTCGSCSGMFTANSMNCLVEALGLALPGN
                     GSVLATHADRKRLFVEAGHLIVDITRRHYEQNDDTVLPRSVASFKAFENAMTLDISMG
                     GSTNTVLHLLAAAHEAENPFSMADIDRLSRKVPVLCKVAPSVANVHMEDVHRAGGIMA
                     ILGELDRAGLIHSDLPTVHSRSLAEALDRWDVKRSNSEAVHRFFSAAPGGVPTQTAFS
                     QERRYDSLDLDRAKGVIRELSHAYSKDGGLAVLTGNLALEGCIVKTAGVDESILSFAG
                     PARIFESQDAAVDGILSGKVKAGDVVLVRYEGPRGGPGMQEMLYPTSYLKSKGLGKVC
                     ALITDGRFSGGSSGLSIGHISPEAAEGGLIGLVEEGDRIEIDIPGRRIELVVPQQDLA
                     KRRAAMEARGKDAWKPAAPRKRKVSTALKAYAALTTSAARGAVRVVK"
     gene            complement(20984..22318)
                     /locus_tag="PLQ11_02570"
     CDS             complement(20984..22318)
                     /locus_tag="PLQ11_02570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HRK23816.1"
                     /translation="MTASETQKPAADHAPSPPFDLPYPRQCDRIATRSQRMFVSTAFA
                     GVSAFLLWANFTELEKVTRGNGRVIPQVQNQIIQHFEGGIVREILVKEGDRVEKGAPL
                     IRIENSFQRAELLQAQLEIRVKSFRLKRLDAEIRGKEFEADAELRKDFPRLVETELGL
                     YETRRKTLTEQIAILDDQFKQKEIEISEFKSRWKNTGRERELVLQRVTSLRRLVNVGA
                     VSSNELIENERTLQQIEGRISDLTHDIPKAEAAMSEISRRRSEARLRFQAEAEKERAD
                     IELQIAKLNETVTAMRDRSERTEVVAPISGTVNKLNVSTIGGVVKSGDQLGQIVPIDT
                     SIAVEARIQPKDRAEVWPGQVAVVKISAYDFSLYGGLKGKVVEVSPDALQDEKGQPYF
                     RIRLEAEGSNFGPDRPVVPGMIADVDILSGRRTIMETLIRPVKALRDNALRQ"
     gene            22451..>22639
                     /locus_tag="PLQ11_02575"
     CDS             22451..>22639
                     /locus_tag="PLQ11_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23817.1"
                     /translation="MSDAGKPDSAKPESKSSDTKTLLDEFRARMAPKAYRWRRPEPSA
                     TGASPALANAAEVPAPGPQ"
BASE COUNT         4015 a         7437 c         7052 g         4135 t
ORIGIN      
        1 gggaccgcgc gcgacttccg tgctgccagg agtacggcca agtgtcactt cgatgggaat
       61 gagatcagcc cgcggcccca gcgaggggcg cccgccgctg ccgatgctgc cgaccgaccg
      121 cgccgctgtt gcctgtggca tcatcaccgc aaagaccgaa ccagaatcca tcgtcggcat
      181 catgatggcg ccgccgctgt cctcttcctc accgaggaat atctggcgcc cgcgccggca
      241 gatctcctgg cgcatgttgg gcgccgaacc ataaccggac tcggccaggc cagtcagcgc
      301 accatagccg ccgccccagc tggaaaagcc cttgtcgagc aactgtgccg tgcggattgt
      361 ccgttcggca ccggaccccg agccgagcac gaccgccagc atcgtgcgcc cgccgcgatt
      421 ggccagcgcc accacattga aaccggaggc gcagacaaag ccggtcttca tgccctccgc
      481 gcctgggtaa cggccgatca gcccgttggt gttgcgcatc acccgcgcgc caagcttgac
      541 cgcgccgatt ccccaataat cgcggaactg cggaaactcg accagcaatg cgcggcccag
      601 aatggcgaga tcgcgcgccg acgagacatg cgaggcgctg tgcaagccat gcggattgac
      661 gaaatggctg tggcgcatgc caaggcgcgc ggcctcttcg ttcatcagcc tggaaaagcc
      721 atcgagcgaa ccggcaacac cctcggcgat caccaccgcc acatcattgg ccgatttgac
      781 catcaacacc ttgagcgcgt tgtcgagggt aatctcctgg cccggacgca ggccgatctt
      841 gctcggtggc tggccggcgg cccgcgccga ggcgatcagc ggggtatcga gactgatctg
      901 gccggccttc atcgccctga gcgccacata ggccgtcatc atcttggtgg tcgaagcggg
      961 gaaccacggc cgcgtcgcct cctcggcatg gatcacctgg ccggacgata catcgacaac
     1021 cagctccggc gctgcgcgaa gcgcggccgg catcaccatc gcggcggcca gaacggccag
     1081 accaagaatg cgtcccatca tgctctttcg catccggtgg taggggatcg cgcccgcatc
     1141 cacacgcttc tcgagttgct gtaactgcgt cgcgccatcc tgaaaagccg gacgacgcac
     1201 cgctgcgcat tttccttgtc cttgtggccc ggctcgatca aattcgcttt gcgcgaaagg
     1261 tgacgctggc caggctgcga cgggcgcttt acattcggac aacacggcca attcaaggca
     1321 caacattacg gagacaatca tgacggcctg catcgttgga tggtcgcata ccccgttcgg
     1381 caagatggat gcggagacgg tcgaaagcct gatcgtccgc gcgacgcgcg aggcattgac
     1441 ccatgccgga atcagcgccg gcgatgtcga cgagattgtc ctcggccatt tcaacgccgg
     1501 cttctcgcag caggatttca ccgcgccgct ggtgttgcag gccgatcccg cttttcgctt
     1561 caagccggca ctcaggacgg agaatgcctg tgcgaccggt tcggcggcga tccatcaggc
     1621 gatcaagtcg atcgaggcaa aggcggcgcg catcgtgctc gtggtcggtg tcgagcagat
     1681 gaccagaacg ccggggccgg agatcggcaa gaatctgctc agggcctcct accttcctga
     1741 agatggcgag acccccaatg gctttgccgg cgtgttcggc aacatcgccg gcgcctattt
     1801 ccagaaatac ggcgataatt ccgatgcgct ggcggccatt gccgccaaga accacaggaa
     1861 cggcgttgcc aacccctatg cgcagatgcg caaggacgtc ggcttcgatt tctgccgcaa
     1921 cgaaagcgac aagaacccct acgtcgccgg tccgctcaag cgaacggatt gctcgctggt
     1981 ctcggacggg gctgcggcgg ttatcctggc cgatgtcgag acggccctgg cgatgcgccg
     2041 ggccgtggcc ttccgctcgc gcgcccatgc gcaggatttc atgccgatgt ccaagcgcga
     2101 cattctcgat ttcgacggct gtcgcgtcgc ctggggcaag gcgctcgagc gcgcccggct
     2161 caggctcgag gacctgtcct ttgtcgagac ccacgattgc ttcaccatcg ccgaactgat
     2221 cgaatacgaa gcgatggggc tgaccggcaa gggcgagggc aaacgcgcca ttctcgaggg
     2281 ctggaccgaa aagaccggcc ggttgccggt gaatgtttcc ggcggcctca aggccaaggg
     2341 ccacccgatc ggcgcaaccg gcgtctcgat gcatgcgatc accgccatgc agctttgtgg
     2401 tgatgccggc gacatgcaac tgaccaatcc gcgcctcggc ggcatcttca acatgggtgg
     2461 cgcggctgta gccaattacg tgtcgatcct cgaacgcatc cggtgatggc ctttagacga
     2521 ttagagggat aaggttggcg gcatgctggt tcttgtcgtc tttctgatcg cctatatatt
     2581 ctcgcagttc tatcgctcgt gcctggcggt gatcgcggcc gatcttgccc gcgatctcgg
     2641 tcttggcccg gccgagctcg gccatctgac cgcgatctgg ttcatcacct tctcgctggc
     2701 acagtttccg atcggctacc tgctggatcg tcatggcccg cgccgcacgg cagcgctgat
     2761 catgctggtg gcggtggccg gagcggcgct gttctcacag gcgaccagct ttgccatggc
     2821 gcttgtcggc atgggtcttg tcggcatcgg ttgcgcgccg atcctgatgg ccgcccttta
     2881 ctattttggc cgcacgaccc cgcccgagcg ctttcccttc tatgtttcga tcctcgtcgg
     2941 agtgggcaat ctcggcaata ttctcggtgc ccggccgctg gcccaatctg ttgctctgat
     3001 cggctggcgc tcaagcctct tgctgatcgc tgtggcaacg ctggctgcag cctgtgcggt
     3061 gtttttcacc gtgcgcgacc ccgaacgggt tgccgctccc aaccctggcg gcaatgcctt
     3121 gagcgaactc tggcaggtca tctcgttccg caccatctgg ccgctgtatc ttttgacctt
     3181 cgtttcctat gccacaatcg ccgccgaacg cgggctgtgg atcggtccgt ttcttgaaaa
     3241 ggtgcatggg cttgatgcgc tggcgcgggg agatgcggcc tttttcatgg cgctcggcat
     3301 ggcgatggga gcgctcactg ccggcccgct ggtcaatgtg ctcggcacga tgaagcgggt
     3361 tgccatcatg ggcacgtccg gagcgattct cggattcggc acgttggcgc tggttcccgg
     3421 cctcggcgca acgctgaccg tcgccatcct gtttctcacc gggctgttcg gtatgtgcta
     3481 cggcatcatc ctcagccatg cccgattgtt tttcccggcc catctgctcg ggcgcggcgt
     3541 gaccttcgtc aatttcctgt cgattggcgg ggcgggggtc gcgctatcgc tgtccggcct
     3601 gttcgttcgg gccgcagagc tgcgcggcga aggccatgcc gcgatcttct caagcctcca
     3661 tatgatcttc gccagcgcgc tggtgatcgc ccttgcctat tacctgacgg caccggcgac
     3721 accccgcgat acccacaaga cgatgtagcc cggcaccaag ggcatgcggc gattagagca
     3781 tgtcgcgact aagtggatgc cggccaatcg caaaatcaat gttataagtc attgataaat
     3841 atcattgttc tattgttttg gtgagtccat caaaacaaca gcgtgatcca ggcgaccacc
     3901 ccggttggtg cactcggcaa gggggttttt cgaccgcttc ggccggaaca ggatcgtcga
     3961 tcaaggaaat ggcgatgaat gcgaccgccc acagcaaatg ttgccggtca tcgtttggcc
     4021 gtgattgtgc cggagagcgc attcgtctag gttgcgcgcg ggtttaggcg ccgcccgcag
     4081 acgaatcggt gcctgatgcc gccgccgccg gcgcttcaga aacaggagcc acggccctca
     4141 atggattttc tgaaaccacg gtatacgccg atgaaccgtc gccgccgcat ctacgaaggc
     4201 aaggccaagg tcctctatga agggccagaa cctggaacgc tgatccagca tttcaaggac
     4261 gatgccaccg cgttcaatgc caagaagcat gaagtgatcg atggcaaggg cgtgctcaac
     4321 aaccgcattt cggagttcgt gttccagcat ctcaacgcca tcggcgtgcc cacccatttc
     4381 atccggcggc tcaacatgcg cgagcaattg atccgcgagg tcgagatcat tccgctggaa
     4441 gtggtggtgc gcaatgtcgc cgctggctcg ctgtcgcagc gtctcggcat cgaggaaggc
     4501 accaacctgc ctcggtctat catcgagttc tattacaaga acgacgcgct caacgacccg
     4561 atggtctccg aagagcacat caccgccttc ggctgggcga cgccgcagga gatcgacgac
     4621 atcatggcgc tggccatccg cgtcaatgac ttcctgtccg gtctgttcct cggggtcggc
     4681 atccgcctgg ttgatttcaa gatggaatgc ggtcgcctgt gggaaggcga catgatgcgc
     4741 atcgtcgttg ccgacgagat cagcccggat tcctgccggc tttgggatat caagtcaaac
     4801 gacaagatgg acaaggaccg cttccgccgc gacatgggcg ggctggtcga ggcctatacg
     4861 gaagttgccc gccgcctcgg catcatcgcc gagaatgagc ggatcaatgg cacaggcccg
     4921 aagctggttc agtagggtca aggtcgagag cattttcgag cgaagtggag tccggttcgc
     4981 gttaagaaaa tgcggaaaaa agcaaaaaga tcgagcattt tccgtgagtt ggaattcact
     5041 gaaattgctc cagacagata ccaacggcgg cctcctgggc cgccgttggc gtttggcgga
     5101 tgcgcgctat ctcagaacag gccgaccacc tcgccggccg cattgaggcc gatctgttcg
     5161 gccgacgggc ttttcggcaa accgggcatg cgcatgatgt cgccacagat cgccacgacg
     5221 aatccggcgc cggctgagag cgacacctcg cgaaccggca caacatgatc aaccggcgca
     5281 ccgcgcaggc tcgggtccgt cgagaaggaa tattgcgtct tggcgacaca gatgggcagg
     5341 tcgccatagc cggccgcctg caaccgcttg aactcggaac gcaccttctg atctgcgatg
     5401 atgtcggacg cgccgtacag cctggtggcg atggtcctca tcttgtccca taacggcatt
     5461 tcagccggat agagcggccg gaaacgccgc ccgtcgagcg cctgcggagc ttcggctagc
     5521 tgggcgacct tgatcgcaag ctcctcggtc ccagccgatc cttccgccca gtgacggcaa
     5581 acgaccgcct cgaccccgag ttcattgcgg caataggact ggattgcctg atgttcggcc
     5641 tggctgtcgc tgccaaaggc attgatcgcc accaccgagg gaacgccgaa actgcggatg
     5701 ttctcgacgt gccggcgcag attggcaagg ccggtagcca gagcggcgag atcttcgcga
     5761 ccaagttcgt ccctggcgag cccgccatgc atcttgagcg cgcgcacagt ggcgacaagc
     5821 accgcagccg acgggaccag ccctgccttg cggcacttga tgttgaagaa cttctcggcg
     5881 ccgagatcgg ctccgaaacc ggcttctgtc accacatagt cgccgagctt cagccccgcg
     5941 cgtgtggcga tcaccgaatt gcagccatgt gcgatattgg cgaacgggcc accatgcacg
     6001 aaggccgggt tgccttccag cgtctgcacc agattggggg ccagcgcgtc cttcagcagc
     6061 acggtcatgg caccggccgc cttcaggtcg cgcgccagga tcggccgctt gtcgcggctg
     6121 tagccgacga tcatcgcccc cagccgttgc tcaaggtctt caagatcgcg tgcaaggcag
     6181 aacaccgcca tgacttccga tgcgaccgtg atgtcgaacc catcttcgcg cggatagccg
     6241 ttggccgccc cgcccaaaga ggaaacgatg gcccgcaacg accggtcgtt catgtcgata
     6301 gcccgccgcc aggcgatccg gcgcgtgtcg aacccaagcc cgttgcccca gtagatgtgg
     6361 ttgtcgacca tcgcggcgag caggttatgg gctgccgaga tcgcgtggaa atcgccggtg
     6421 aaatgcaggt tgatctgctc catcggcatg acctgggcat ggccgccccc cgccgccccg
     6481 cccttgacgc cgaaacaggg cccgagcgag ggctcgcgca ggcagatgat ggctttcttg
     6541 ccgatacggt tgaaggcatc gccgaggccg acagtggtgg tggtcttgcc ctcacctgcc
     6601 ggggtcgggc tgatggcagt gaccaggata agccttccgt caggacgctc ggaaaggccc
     6661 gccagatggg acagcgccac cttgccgaca tggtgcccat acggggacaa ggcgtcggcc
     6721 ggaatgccga ggccggcagc gacagcgtgg atcggcttga ggctggcagc gcgggcaatt
     6781 tcgatgtcgg tggtcatggc tcaacgcccc gcagaaagtt cggatcattt atttcagctt
     6841 ttttccggtt gttgccaagt ccgcgcgctt gccgatcctc cttttgatcg aacgtattgc
     6901 gtcctcagga tggccaaagc tatggtctgg ccggtgaagc gttctgccaa cgcgtagcga
     6961 ggtatcccgg gagccagcgg cacacgatgg acgacagcac tttggaacga ccgaaggccg
     7021 gtaaaggccg gcgctggcag acccggggcc gcatcgccga tcccgcagca gcgcgcgaca
     7081 tcgaccttgt tctgggccat gaacccgtcc gccgtgatca cctgatcgaa tatctccatc
     7141 gcatccagga tcgtttcggc gctctcatgc cgcgtcacct caaggcgctt gccgaccgga
     7201 tgcggctttc ggaggtcgag gttttcgagg ttgccagctt ctaccaccat ttcgatatcc
     7261 gtcgcgacga tgacgccggc cctgccgcga tcaccatccg tgtctgcgat tccgtctcct
     7321 gcatgctggc gggcggcgaa gcgctggtcg agcgcctgac cgatctggtt gaccccgcac
     7381 atgtgcgcat catgcgcgct ccctgcatcg gccgctgtgc cgaagctccg gccgcgctga
     7441 tcggtaaacg cgcggttggt catgccacgg ctgaacggct ccgcgatctt gccgcagcag
     7501 gaaccgtgac gccggagccg gttgcagcta cccgcctggc ggagtatcag gccgccggcg
     7561 gctaccggct tcttgccagc atgcggtccg gcgcattgac gccggcagcg atcactgaag
     7621 cgctctcaga cgccggattg cgcggcctcg gcggggccgg cttcccggcc gggcgcaaat
     7681 ggtcgatcgt gcggtccttt cccgcccccc ggctgatgac gatcaatgcc gacgagggcg
     7741 aaccggggac gttcaaggat cgctgcctcc tggaagccga tccgcatcgt gtgctcgagg
     7801 gagccctgat cgccgcccag gcggtcgagg ccgagcgcat ctatctctat ttgcgcgatg
     7861 aatatgcgca tctgcatcaa ttgcttgggg cagaaatcga tgcactggcc cgggccgagt
     7921 tgatcaagcc gggcttcatc gaattgcggc gcggcgcagg cgcctatgtc tgcggcgagg
     7981 aaagcgcgat gctggcctcg atcgaaggac gtcgtggcct gccgcgccac cgcccgccct
     8041 atattgccga agccgggctg tttggccgcc ccacgctcaa ccacaatgtg gaaacactct
     8101 actggatccg cgacattctc gaccatggcg cggaatggtt tgccggtcag gggctgaatg
     8161 gcgccagggg cctgcggtct ttctctgtct cgggccgcgt ccgtttcccg gggctcaagc
     8221 gcgcccccgc cggcatcacc ttgcgcgaac ttgtcgatca gcactgcggc ggcatggcgg
     8281 aaggccacag gctggccggc tatctgcccg gcggcgcatc aggcggcatg ctgccggcct
     8341 cccgcgccga tctgccgctc gaattcggcc ggctggaacc gcatggctgt ttcatcggat
     8401 cgcacgccat cgttgtcttt tccgatcagg acgatccggc agcgattgct ctcaacctga
     8461 tgcagttttt ccggcatgaa agctgcggcc agtgcacgcc ttgccggctg ggcacggaaa
     8521 agctggtggc gctgatggag cagggcgccc ttgatcgcgc actcgttgcc gatatcgatc
     8581 atgcgatgcg agaagggtcc atctgcgggc tcgggcaggc ggcgcccaat ccgctcgcct
     8641 gtctcatccg ctattttccg gaggccctac gatgagcgat ccgatccggt tttccatcga
     8701 cggtcgcccg gtagacgccg aacccggcga gacgctgtgg caggtcgcca aacgcctggg
     8761 gatcgcgatt ccgcatctct gccatctcga ccagccaggg taccggccgg atggcaattg
     8821 tcgtgcctgc ctgtgcgaga tcgaaggcga aaggaccctg gccgcatcct gcatccgcaa
     8881 gccggccccg ggcatggtgg tccacaccgc aagcgaacgc gtcagtcggg cgcagcgcac
     8941 ggtcttcgaa ctgatcgagg ccggtcttcc ggcgcgcgcc ggggcaccgg acccggatgc
     9001 accgttctgg cactggtcgg agcaggtggc ggctccgcag gggcgattgc ccggccgcca
     9061 tggagtggtt tcgcccgagg ccgccggact ttctctgcac gattcctccc atgcggcgat
     9121 tgcggtggat ctgaccgcct gcattgcctg cggcctctgc gaaagggcgt gccgcgaagt
     9181 gcaggtcaac gacgtcatcg gcatggccta tcgtggcatc cagacccgac cggtctttga
     9241 tgcggatgat ccgatggggg cctccacatg cgtgggctgc ggcgaatgtg tgcaggtgtg
     9301 cccgaccggt gccctcctcg aaaagaccgt gcagaccagc atcaagcaat cgggacgcct
     9361 cccgatcgac cgcgaagtcg atacgctctg tccctactgt ggcgttggtt gcctgacccg
     9421 cgtggccgtg gccaatgacc gtatcgtccg tgttgacggg cggcccggca gggccaacga
     9481 cagccggctg tgcatcaagg ggcgcttcgg gtttgattat gtccaccatc cccaccggct
     9541 gatccgtccg ctgatccgcc gcgacgatgc accgaagcgc ggcgatgcca gccttgatcc
     9601 gtccaacccc tggcccacct tccgcgaggc gagttgggag gaggcgctgg agcgggcagc
     9661 ggccggcttc cgccgcattc ttgaccggca tggcggtgct gcgcttgccg gcttcggttc
     9721 ggccaaatgc tcgaacgaag aagcttatct gttccagaag ttgatccgac agggtttcgg
     9781 caccaacaat gtcgaccatt gcacccggct ctgtcatgcc tcctcggtca cggcgctgat
     9841 ggaaggcatc ggttcgggcg cggtctcggc gcccttcaat gcagcgctcg atgccgattt
     9901 catcatgatc atcggcgccc gcccgaccga gaaccacccg gtcgcggcca cctatgtcaa
     9961 ggaagccgtg cgcaccggca agaccctggt cgtcgccgat ccgcgcggtt cggcactgat
    10021 gcgtcacgcc acgcatgcct tgcgcttcag gccgggcacc gatgtggcgc tgctcaatgc
    10081 catgctgcac gtcatcgttg cagagaacct gtatgatccc gactacattg ccgctcatgt
    10141 cgacaatttc gacgcgttga aagccaatgt tgccgaatgt tcccccgagg taatggcgcc
    10201 gctttgcggc attgacccgg gcatgatccg gaccgtcgcg cgcctctatg cccgctcgcg
    10261 cgcctcgctc attttctggg gcatgggcgt ctcccagcat gtgcatggaa ccgacaacgc
    10321 ccgctgtctc atcgcgcttg cgctgattac cggccagatc ggccggccag gcacggggct
    10381 tcatccgttg cgcgggcaga acaacgtgca gggcgcctcc gatgccggcc tcatcccgat
    10441 gttctacccg aactatgggc ctgtcgattc tcccgatctc accgcccgtt tccagcaggc
    10501 ctggggccgc ccgctcgatc cggcgcgcgg cctgaccgtc gtcgaaatcc tgcacgccat
    10561 cctggccgat cgcatcaagg gcatgctgat catgggagag aatccggcga tgtccgaccc
    10621 cgaccagatc catgcccgcg cagccctggc ccggctcgag catctggttg tgcaggatat
    10681 cttcctcacc gagacggcct ggcacgccga tgtggtgctg ccagcctccg cccatgccga
    10741 aaagctcggc accttcacca ataccaaccg gcaggtgcaa ttgggccggc cggtcctgcc
    10801 tctgccgggc gaggcacgcc ttgatatcga ccttctggtc agccttgcca accggttggg
    10861 ccttggctgg tcctatggcc agcagcccca tcccgaaggg ggtgagaccg gtgtcgaggt
    10921 cagcgccgtc tatgaggaga tgcgtgggca tatggtgtcg ctcaagggcc tgccatggtc
    10981 gcgcattgcc gccgaaggca gcgtcaccta tcccgccgcc gatgcgacat cggcaggccg
    11041 agaggtcctg ttcggcgacg gcttcccccg cgccggcggt cgtgcccggc ttgttcctgc
    11101 ccgcgtttcg ccgccgaacg aggcgccgga tgagcgctat ccgctggtat tgacaacagg
    11161 tcgcctgctt gagcactggc acaccggttc gatgagccgc cgggcgtcag tcctggccgg
    11221 gctggagccg gaaggtctgg ccgcaatgca tccggacgag atcagccgac gcgggctcat
    11281 tgccggcgat cgcgtgcgca tcgcaacccg tcgcggggcg atcgaggccc ggctgcgggc
    11341 ggatcgcgat gttgcgccgg gcatggtgtt cgtgcccttc gccttcaacg aagcggcagc
    11401 gaatgtgctg accaatccgg cgcttgatcc tttcggcaag atccccgaac tcaagttcgc
    11461 cgccgcgcag gtgaccgccc ttggctgaga ctgcaccaaa ggaagcgtcc agcccctgca
    11521 tcaagctgtg caccatcgat ccgggcactg ggctctgcgc cggatgcgga cgcacgcttg
    11581 aggagattgc cgcctggtcg atgatgacgg atgccgagcg catcgcgctg aacgccgcac
    11641 tgccggatcg tttggcgagg cgtcgtcggc cagtggcctg ccggctgcca acctcgccct
    11701 cgcggacttg acttgggcgc cgccgccggt cacggctctt ggctcattcg tcatccattc
    11761 ccaggccgac atgctgccgc aggacaaact cgacgccatt ctcgcccgct acgacgagat
    11821 caacgcgcat ctggccgaga cccaggactc gcagctgatc atccggctgt cgaaggagct
    11881 tgccgaaatc gaagcgctgg ttggcaagat tcgcgcctgg cgcggctttg cggcagcgga
    11941 ggccgaactg ctccagctgg ccgaggaccc cgagatgcgg gcgcttgccg aggacgacct
    12001 cgcccggttg cgcgccagtc acatcgaggc agaacgcgat ctgcggcttg ccctcatccc
    12061 ccgggacgcg gccgacgaaa agggcgccat tctcgaagtg cgggcgggaa ccggcggcga
    12121 cgaggcggct ctgttcgccg gtgacctgtt ccggatgtat cagcgctacg ccgacttcaa
    12181 gggctggcgg gtcgaggtca tttcgctcag cgaaggaacg atgggcggct ataaggaaat
    12241 catcgccgag attcagggcg tcggcgtcta tgcgcatctg aaattcgaat ccggggttca
    12301 tcgcgtccag cgtatcccgg tcaccgaggc aggcggacgc atccatacat cggccgcgac
    12361 cgtcgcggtc ctgcccgagg cggccgatgt ggacatggat attgacgaca gcgacctcaa
    12421 gatcgacacc atgcggtcgg ggggagccgg cggccagcat gtcaacaaga ccgaatcggc
    12481 ggtgcgcatc acccacattc ccactggcct tgccgttgtg gtccaggagg agcgctccca
    12541 gcacaagaac aaggccaagg cgctcgccat cctgcgcgcc aggctttacg accgcgagcg
    12601 cgaacggctg gccagcgagc gcgccgccga tcgcagggca caggtcggat cgggcgatcg
    12661 ctcggagcgc atccggacct acaatttccc gcaaggccgc ctgacggatc atcgcatcgg
    12721 cctgaccctc tacaaactcg agcagatcat ggagggcagc gcgttcgacg aggttgccca
    12781 ggcgctgatc accgagcatc aggccgccct gctggcaggc gaagaccgtg gtcatggctg
    12841 aagcgcttct gacagcgacc acgccctatt ccgaagcgct tggccagatg accaccgtgc
    12901 ttgaggccgc tgggctggcc gaagcacgac gcgaggcacg cattctgctc tgtgcggtcc
    12961 ttggtgtcgg agccgaaatc ctgatcctgg cccccgatgc cccgctcggt tcggccgccc
    13021 cagccgttca ggatgcgttg cgccgccgat ccgcccgcga gccgctggca cggatcaccg
    13081 gacatcgcga gttcttcggc cttgatatgg aactggcccg atcggccctg atcccccgcc
    13141 ccgataccga aacactcgtc gaagccgtgc tggatgaact ggggcagagc ggccgcgatc
    13201 aggatgccct gtcgttgctg gatctcggaa ccgggtccgg cgcaatcctg acggcccttc
    13261 tcagccggct gccgcaggca ggcggggtcg gggtcgaccg caccatcgac atcctgccgc
    13321 acgccgcccg gaactgcgcc cgccattgcg gaaccggcag ggccttgttc atcgccggga
    13381 actggaccga cgcccttggc ggaacctttg acgtgatcgt ctccaacccg ccctatatcg
    13441 ccacggccga tatcgccggc ctcgacccgg aggtgcgcga ccatgatccc cggctggcac
    13501 tcgatggcgg cagcgacggc tatgccgcct accgtgcgct ggcccgggct atcccgcccc
    13561 ggctagcgcc gggcggcctt gtcggccttg aacttggtgc aggtcaggcg tccacggtcc
    13621 gtgcaatctt cgccgcggca ggcttgggca gcctctcctg caaacgcgac cttggcggtc
    13681 acgaacgggc cttgctgctc cgccgggcgt aataccagcg gccctgacaa ttgaccgtta
    13741 gatcacgccg cattttgatg gaatcatcaa aatgcggata acgtgatcgg tatcaataag
    13801 ttagggcatg tcttctgcaa ttaaccggta tccacttaat cgcgacatgc tctaggggca
    13861 attgtcaggc ggctggttag gagcgaatgc gacgccgagc aggaccgaag gcccggttgc
    13921 aagggacggc ctggaaaatg accggcaggt catttgtgag ggccactggt ataacgcagc
    13981 gctcgcccgc tgctcagatc gccgacaggg tccaggcatg cgggaaggtg ccataatcgg
    14041 aggtcgtgac cccgttgcgc gtggtgccga gataggagtc gggcggcggc acgaccttga
    14101 cccgcagaac agccgtttca cccaccttaa gcaggcccga atgcaccgca gccgccccga
    14161 ggatcgaatc gcgcgtgtag gtatcggtgc cccagacctg tccttctgcg acaccggtga
    14221 tgcggaaata gaacgtggct cccaccgttt cgcacaatcc atgcatgttg gtcggcccgt
    14281 cggcggcaat ccggcttttg tcgagcaagg tcctgagccg gcgcttgctc agctggccgc
    14341 gcgtgtcgaa ataggcaaca agctcctgca cgaccctcat gatgaccccc gtgacatggt
    14401 gatgacgcgc ccgtaggtcg gcgcaaaact ggtggaatcc cccggcaccg cccatagcac
    14461 cgcatagggc atcggctcgc gcgggtaggt gatgtcgccg tcggtgatga cgatcgcggc
    14521 ggtgacgcgc gggtcctccg caagctggtg cagcgccggc gaaagatcgc tgccgccgta
    14581 gccacgcacc tcgtattcgg ccagttcggt cgccgacagc attgcatcgc cggtcaccac
    14641 cgtatcgcac tggatgacgc ggatttcatc gacgccggtt gcctcgcaga aatcggcaat
    14701 cgcgccgagt gcgtagggga tctcgtcact catcgaaccc gatgtatcga gcacgacatg
    14761 cagcatcatc gaataacgct gacgccccgg cagcaccaga tcagcccggt cagcgccccg
    14821 ccgcgaggcg cgtgtgaagg tgcgctcgcc gggtgatacg ccttccagcc agcgttgcag
    14881 cgccacctgc caaggggtgc ggtagatgcc gcgcagcgcc ctgacctgcc gctgcgatcc
    14941 gcccgcctcg gtgccgcgcc ccttcatcag cccgagcgcc ttgccgagcg ccatgccctt
    15001 tgccgccaga tcgcggatgg cgcgtgcgcg ctccatctgg tcggccacat cgtcaggaaa
    15061 catggcccgc tcgcggtcgg catcgagcac atcgcccata tcccctgcct gtccctgacg
    15121 agccgaattc agcacctgtt cgatgctgac gatctgccct tcccagacgc gcgaccgcga
    15181 ggaattggac tggttgcgtc gcatctccag aacgatctct tcggccgaac gttcgcgcgc
    15241 tccggccatg tcaagaccgc cggcggggat caccccgagc cccagcgcat gccgcagcat
    15301 gtcgttgatg atgtaatcat gggcatagtt gaactcgagc cggccggaac cgaccgcccg
    15361 gtcatgggtc cgcagcgcca gatgcagcag ttcatgggcc agcacgaaga ccagatcgtc
    15421 attggataac cgggccgtga agcgggaatt ggcaaccaga cggcccgagg cgaacacgcc
    15481 catcgtcggc acgctgtcat caacagccac ccgcactgcc gccgcaagcc cggtcaggtg
    15541 cggaaacggc accgtgacca tgcgcaggcc ccgggtcagc cgttgcaggc tcgcccgttc
    15601 aacatccgct gtttgagccg gccggctcat gcggcagcga ccttacgcaa caacgccagc
    15661 atcgcctttt cggcgccgag cgcgccccat ttctccacca tgtcggtcag cacgatcagc
    15721 tgatgttctg tcgacagact gccgaggaag cggttgatga cgcgcggcgc aaaaccgtcg
    15781 gcccgcccgt ctcggacgaa ctggcggagg cagtcgagga tgaaccagcg ggcggaatcg
    15841 ccgcgcggca gcaggtccgg ttcgtggatg tagcgcgcca gcggctgcac cggaccgata
    15901 ttgtcctcgg cgagcgagca gaacaccgcc gcatcctccg gcgacagccg gccaaaggcc
    15961 agcgcccgcc gcgtgtcatt ggtcagcaag ccggcctttt ccgccagatc gagcgcccgc
    16021 gacagcagcg accaggcgcg tggcgtcgag aacggcaccg gatcggccgg caccgcccgc
    16081 atcagcgcat caggaacggt ggtgataaac gaacggatgt cctgccttat gccgctgcgc
    16141 tgtgcccagg ccagccactc gtcggtatcg cagcggatct gcaggatcgt gacacgattg
    16201 accagcgccg aactcatcgc ccggaccagt gcccggtcct gcaaccggtt gccggcggcg
    16261 acaacccagg tgcccggcgg cagggcatgt tcccccagcc gccgttccag cagcagcgaa
    16321 tagaacgcct tctgcacatc cggcgcgcag gccggcaatt catcaagaaa caggcagaag
    16381 ggctcgggcc gctccggcag gagaatgcgc ggcgggcaga acacgctgcg ctcaccgaca
    16441 atgcgcggca caccgctgac gtcttcgggc gcgatctgtg ttccaagcag ggaacggcag
    16501 ggcagcccgg cttcgcgagc cgcctcatag accatgtccg acttgccgat gcccggcgcc
    16561 gacagcagca gaaaactctg ctcatgcgcc atgcagcgga tgagcgtctt ggcctgccgg
    16621 agcgtgacca cctcattgaa cgcagcctga cccgtggtca tgcgctatcc tcccgatctg
    16681 gcatcgcact tatgctttac atgagactaa gtatcgtccg aatggcggac aaggccaaga
    16741 actatatttt tctctatgtg agcaaaaatt gggatgcggt tcatacttgc cgcttcagtg
    16801 ccgacagcgg cgatccggcc aaaaaaaccg gcggtttgat ctaagtctct tgcctctcgc
    16861 tgcaagcggt ggaagcatcc ggggtgaagg acgctggtca gcgtcgcatc gaacagactg
    16921 tttttgggag aatacgatgc cggagaatac caacgcagga cgaattgcgg gaccgcggct
    16981 gattgcgctc gtcggtccct ttcagagtgg caagaccacc ctgctcgaga acctgctggt
    17041 gcgctcggga gcactggccc gcgaaggatc gatcaaggat ggctccagcg tcggcgattc
    17101 cagccccgag gcacgcgccc attcgatgag cgtcgagccc aacattgcct cggccgacta
    17161 tctgggtgac cgcttcacct tcgttgactg ccccggctcg atcgaattcc tgcatgacat
    17221 gcgcaatgtg ctgccgctct gcgatgcggc catcgttgtc tgcgaggcca acccgagcaa
    17281 ggtcccggcc cttcagctga tcctgcgaga gcttgaagac ctcggtatcc cgcgccttct
    17341 gtttctcaac aagatcgaca cggcaaccca gcgcgtgcgc gaaaccctgc aaatgttgca
    17401 gccagcttcg cgtgtgccgc tgctgctccg ccagattccg atctggcaca acggcattgc
    17461 caccggcttc atcgacctgg cgctcgaacg cgccttcctc taccgcgaac acgcgccttc
    17521 ggtggttatc gacgtgccgg ccggagaggt tccgcgcgag aaggaggccc gctattcgat
    17581 gctcgagcgg cttgccgact acgacgacag cctgatggag gaactgctca ccgacatcga
    17641 gccgccgcgc gatcaggtgt tcgacgacct ggcccgcgag ctgcgcgagg ggctggtggt
    17701 gccggtattc atcggctcgg ccgagcgcgg caatggtgtc acgcgcctgc tcaaagcgtt
    17761 gcgacacgaa agcgccagca tcgccgccac gcgtgcgcgg ctcggcatcg gtgacagcgg
    17821 gccggcgctg gctaattcaa ttcgctcggt gcataccgct cacggcggca agctgaccct
    17881 ggcgcgcgtt ctgcgcggct cgtttgtcga gaacaccaag gttttgagcg ccggcggcca
    17941 tgaggaccgc atcggttctg tctcccgcat gattggcacg gccgcaacaa ggctgcctgc
    18001 ggcccaggaa ggcgacaccc tcgccttcgg ccgccttgat catgtggcga ccggcgaaac
    18061 cttcggcgat gtcaaaggcc cggcgccgcg ccctgcggtg gcccgcgccg agcccccgca
    18121 gccggtgcac ggcatggtgc tgacgatccg tgaccacaag gacgaggtca agcttgccgc
    18181 cgccatgacc aaactcaccg atgaggatcc tgccctgcac ttcgtccagg accagacctc
    18241 cggcgaaacg cggctgatgg gccagggcga gatgcacctg cgcgtcgcgc tggaacggct
    18301 gaccaagcgg tttggcgtcg cggtggatac acggcgcgcc tcggtcgcct atcgcgagac
    18361 gatccgccat caggtgagcg tgcgcggccg gcataagaag cagtcgggtg gccatggtca
    18421 gtttggtgat gtcgccctga cggtgcggcc gctggaacgc ggcgccggct tccagtttgc
    18481 cgaaaccgtg catggcggca ccgtcccacg ccagtatttc tcggccatcg aggctgggtg
    18541 ccgcgacgtc tgcgaggaag gcccgctggg ctttccggtt gtcgatgtgc atgtcacctt
    18601 gaccgaagga tcgtatcaca cggtcgattc ctccgacatg gccttccgca tggcggcgcg
    18661 gctgggcatg catgaagcac tcaagaccgc aggccctgtg ctgcttgagc cgatcctgtc
    18721 cgtgaagatc cacgttccga gcgaggccct ttcgcgcgcg accggcattg tgaccggacg
    18781 gcggggacag attctcggct atgatgcccg cgccggctgg gctggatggg acgaactcaa
    18841 cgcgctgatg ccggaagcgg aaatgaccaa cctgatcgtc gacctgcgct cggcctcggc
    18901 cggcgtggcg ggctatacat ccagcttcga ccatctggcc gagatcagcg gcaaggcggc
    18961 ggagaccatc atcgccgcac gcgcgcatgc caaggccgcc tccggccatg gacatggtgc
    19021 ctgaccgttc ggcgggcgcc ggcctggttc cggcgcctgc acaaccctat ttcaccacgc
    19081 gcaccgcgcc gcgcgctgcg ctggtggtca gcgctgcata ggccttgagc gccgtcgaga
    19141 ccttgcgctt gcgtggcgcc gccggtttcc atgcgtcctt gccgcgtgct tccatcgcgg
    19201 cgcggcgctt tgccaggtcc tgttgcggca cgacaagctc gatccgtcgc cccggtatgt
    19261 cgatctcgat gcgatcccct tcctcgacga ggccgatcaa tccaccctct gccgcttccg
    19321 gcgagatatg gccgatcgac agtccggacg atccgcccga gaaacgcccg tcggtgatca
    19381 gcgcgcagac cttcccgagg cctttggact tcagatagct cgtcggataa agcatttcct
    19441 gcatgcccgg cccgccgcgc ggcccctcgt agcggaccag gacgacatcg ccagccttga
    19501 ccttgccact gagaatgcca tccaccgcag catcctggct ttcgaagatg cgcgccggac
    19561 cggcaaagct gaggatcgat tcatcgaccc ctgccgtttt gacgatgcag ccttccagcg
    19621 ccagattgcc ggtcagcacc gccagcccgc catccttcga ataggcatgg ctcagttcgc
    19681 ggatgacgcc ttttgcccgg tcgagatcga gactgtcgta gcgccgctcc tggctgaacg
    19741 ctgtctgtgt cggaaccccg cctggtgccg ccgaaaagaa ccggtgcacg gcttcggaat
    19801 tgctgcgctt gacatcccag cgatccagcg cctcggccag actgcggctg tgcacggtcg
    19861 gaaggtcgct gtggatcagc ccggcacgat ccagttcccc cagaattgcc atgatgccgc
    19921 cggcgcgatg cacatcttcc atgtgcacat tggcgaccga aggcgccacc ttgcacagca
    19981 ccggaacctt gcgcgacagc cggtcgatgt cggccatcga gaacgggttc tccgcctcat
    20041 gcgcggccgc aagcagatgc aggaccgtat tggtcgatcc gcccatcgaa atatccagcg
    20101 tcatcgcatt ctcgaacgcc ttgaacgatg ccaccgaacg cggcagcacg gtatcgtcgt
    20161 tctgctcgta atgccggcgg gtgatgtcga cgatcaggtg ccccgcctcg acgaacaggc
    20221 gcttgcgatc ggcatgggtg gcgagcaccg aaccattgcc gggcagcgcc agccccagag
    20281 cctcgaccag gcagttcatc gaattggcgg taaacatgcc cgagcaggaa ccgcaggtgg
    20341 gacaggcgga acgctcatag gcggagacgg ccgcatccga cacgctgctg tccgctgctg
    20401 cgatcatcgc atcgatgaga tcgaccttct tggtcacgcc ttcggcgaca aacttgcccg
    20461 cctccatcgg cccgccggag acgaacacag taggaatgtt cagcctgagc gccgccatca
    20521 gcatgccagg cgtgatcttg tcgcaatttg agatgcagac cagagcatcg gcacaatggg
    20581 cattgaccat gtactcgacc gaatcggcga tgatctcgcg cgaaggcagc gaatagagca
    20641 tgccgtcatg gcccatcgcg atgccgtcat cgaccgcaat ggtgttgaac tccttggcga
    20701 cgccgcccgc cttttcgacc tcgcgggcga ccagctggcc aagatcgtgc agatgcacat
    20761 ggcccggcac gaactgggtg aaggaattgg caatggcgat gatcggcttg ccgaaatcgc
    20821 cgtccttcat gccggtggcc cgccacaggc cgcgggctcc ggccatattg cggccatgcg
    20881 ttgaggttct tgaacgatac gcgggcatgg aaacgttcct gacagacaga tggatcaggg
    20941 cttgcctaac accacccgcc ggccggaggc aattgtcctg tagctattgg cgaagtgcgt
    21001 tgtccctcag cgccttgacc gggcggatca gcgtttccat gatcgtccgc cggcccgaaa
    21061 gaatatcgac atcggcgatc atgcccggga cgaccggtcg gtccggaccg aaattcgagc
    21121 cttccgcttc cagccggatg cggaaatagg gttgtccctt ttcatcctgc agggcgtcgg
    21181 gcgaaacctc gacgaccttg cccttgagcc cgccatagag cgagaaatcg taggccgaga
    21241 tcttcaccac agccacctgt ccaggccaga cctcggcacg atccttgggc tggatacggg
    21301 cctcgaccgc gatcgacgtg tcgatgggca cgatctgtcc aagctgatca ccggatttga
    21361 cgacgccgcc gatggtcgag acattgagct tgttgaccgt cccggaaatc ggcgccacca
    21421 cctccgtgcg ctccgagcga tcgcgcatcg cggtcaccgt ctcgttgagc ttggcaatct
    21481 gcaattcgat atcggcgcgt tccttctcgg cctcggcctg gaatctcaga cgcgcctccg
    21541 agcggcggcg ggagatttcg ctcatcgccg cctcggcctt ggggatgtca tgcgtgaggt
    21601 cggaaatccg tccctcgatc tgttgaaggg tacgctcgtt ctcgatgagc tcattggacg
    21661 agaccgcccc gacattgacc agccgcctga ggctggtgac acgctgcagg accagttccc
    21721 gctcgcgtcc ggtattcttc cagcgcgact tgaactcgga aatctcgatt tccttctgtt
    21781 tgaactggtc atccaggatg gcgatctgct cggtcagggt tttcctgcgc gtttcgtaaa
    21841 ggccgagctc ggtctcgacc aggcgcggaa aatctttgcg aagctcggca tccgcctcga
    21901 attccttgcc gcggatttcg gcatccagcc gtttgagccg gaacgatttc acccggatct
    21961 ccagctgggc ctgaagcagt tccgcccgct ggaacgagtt ctcgatgcgg atgagtgggg
    22021 cgcccttctc gaccctgtcg ccttccttga ccagaatctc ccggacaatg cctccctcga
    22081 aatgctggat gatctggttc tgcacctgcg gaatgacgcg gccattgcca cgtgtcacct
    22141 tttccagctc ggtgaaattg gcccaaagga ggaatgcgct gacccctgcg aaggccgtcg
    22201 agacgaacat ccgctgggag cgcgttgcga tacggtcgca ttgcctggga tagggcaagt
    22261 cgaacggcgg cgacggagca tggtctgcag caggcttttg tgtctcggaa gcggtcatgg
    22321 ccgcatctga gcagaggaaa gtttacggct tgttaagctt ggcgccccac catgcactat
    22381 ccatttgtta acgcttgagc cggccgcacg cgccctggtg catgtgcctc cgggagggcc
    22441 aacccccgcc atgtctgacg ccggaaagcc cgattctgcc aagcccgagt caaagtccag
    22501 cgatacaaag acgctgctgg acgaattccg tgccaggatg gctcccaagg cctatcgctg
    22561 gaggcggccc gagccttcag ccaccggggc ctcacccgcc ttggcgaatg cggccgaggt
    22621 gccggcaccg ggcccacag
//
LOCUS       DAOMXG010000020        22633 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_1398_length_22633_cov_9.496855, whole genome shotgun sequence.
ACCESSION   DAOMXG010000020 DAOMXG010000000
VERSION     DAOMXG010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 22633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22633
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1398_length_22633_cov_9.496855"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..270)
                     /locus_tag="PLQ11_02580"
     CDS             complement(52..270)
                     /locus_tag="PLQ11_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003611967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HRK23818.1"
                     /translation="MRQPMSEAERQALTHELEQHRQEHRDLDDAILALEALGAPDKLQ
                     IQRLKKRKLLLKDEITRLSDRLTPDIIA"
     gene            481..672
                     /locus_tag="PLQ11_02585"
     CDS             481..672
                     /locus_tag="PLQ11_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HRK23819.1"
                     /translation="MSVESHLAQLERKHQALKDAIAAELAHPACDDAKVAAMKRKKLL
                     LKDEIAKLKPQVQAPSALH"
     gene            892..2925
                     /gene="tkt"
                     /locus_tag="PLQ11_02590"
     CDS             892..2925
                     /gene="tkt"
                     /locus_tag="PLQ11_02590"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRK23820.1"
                     /translation="MAASLPSLRVFQDLRMPTRAQHDLMANAVRALAMDAVEAAKSGH
                     PGLPMGAADVATVLFRDHLKFDPADPAWPDRDRFVLSAGHGSMLIYALLHLIGVKQAS
                     LDELKAFRQLGSKTAGHPEYGHLAGVETTTGPLGQGIANAVGMALAERLMAARHGGKL
                     VDHNTYVLAGDGCLMEGISQEAIALAGHLKLNRLIVLWDDNNITIDGKVSLADSTDQL
                     KRFEASGWRAERVDGHDPVAISAALDRAKAADRPSLIACKTVIGYGAPTKAGSEKVHG
                     APLGTDELAATKKALGWPHGAFVVPPEVYALWQATAKRSAGARRAWTQRLEATGAKKR
                     AAFEQALAGTQPKELARALKGLKQQAAVTTAEIATRKTSEMTIDAIAGAMDAFVSGSA
                     DLTHSNFTQGKLLKPVKPGDFSGGYIHYGIREHAMAAAMNGIALHGGLIPAGGTFFVF
                     SDYCRPSIRLAALMGLRVLHVMTHDSIGVGEDGPTHQPVEHLASLRAIPNCTVLRPCD
                     LTETVEAYEVALVAARTPTVFALSRQNLPQLRKSYTPKNLTALGAYELMPAEGKAQVS
                     LFASGSEVAMAIEARRLLAEKGIAARVVSVPSHDLFAAQPEAYRRKVIGRAKVKVGIE
                     AGIRQGWDFIIGSDGLFIGMNGFGASGPAAKVYQHFGITAQAVADAAAQRLAR"
     gene            2992..4002
                     /gene="gap"
                     /locus_tag="PLQ11_02595"
     CDS             2992..4002
                     /gene="gap"
                     /locus_tag="PLQ11_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003523203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRK23821.1"
                     /translation="MAVKVFINGFGRIGRNVLRAIIEGKRKDIEVVGINDLGPVETNA
                     HLLRYDSVHGRFPADVKVEGESLVINGQKIRVTAIKDPASLPHKELGVDIALECTGIF
                     TAKDKASAHLKAGAKRVLVSAPADNADYTVVYGVNDAGLTKDHLVVSNGSCTTNCLAP
                     VAKVLNDSFGIVHGFMTTVHAYTGDQPTLDTMHKDLYRARAAALSMIPTSTGAAKAIG
                     LVIPELKGKLDGSAIRVPTPNVSVVDLKFVSKKKVTVEKINDAILKAAKRGPLKGILA
                     VTAEPNVSSDFNHAPASSTFALDQTKVMDEKFVRILTWYDNEWGFSNRMVDSAVAMGK
                     LI"
     gene            4056..4622
                     /locus_tag="PLQ11_02600"
     CDS             4056..4622
                     /locus_tag="PLQ11_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23822.1"
                     /translation="MNRRSFPFLLPATAAMLAMVLAGPAVAVTDAATGFGIAPPAPFT
                     AERSAANAEDVAFSIRSPDGQPPAAGTGALCRAGFKAAATSSGADQPTLNAKLDRPPV
                     LDEMRKPLKPRYVIEREDLFQLGEARGIAYTAIPRAGPNADTVRVVLSFVETPKGRVS
                     LVCATPAPHLEAARPVFEAIRATLILPK"
     gene            4632..5825
                     /locus_tag="PLQ11_02605"
     CDS             4632..5825
                     /locus_tag="PLQ11_02605"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRK23823.1"
                     /translation="MSQTFRTLDALAPKGKRVLVRVDLNVPTENGRVTDATRLDRILP
                     TINDIAGKGGRVILLAHFGRPKGRDASQSLKPVAEELARRLGKPVAFAEDCIGDVAAR
                     AVAAMKDGDVLCLENTRFYKEEEKNEPGFVAELARLGDAYVNDAFSAAHRAHASTEGL
                     AHKLPAYAGRTMEAELKALDAALGNPKKPVVAIVGGAKVSTKLDLLGNLVKKVDILII
                     GGGMANTFLAARGVNVGKSLCEHDLVATAREIEQKAKAAGCEIVLPVDALLAKEFKAN
                     PGHRAAGVADVKADEMILDAGPKSVAVVEAKLKSAKTLVWNGPFGAFELAPFDTATVA
                     AAKTAAALTKAGSLVSIAGGGDTVAAMNHAGVADDLTYISTAGGAFLEWMEGKALPGV
                     EALKR"
     gene            5869..6141
                     /locus_tag="PLQ11_02610"
     CDS             5869..6141
                     /locus_tag="PLQ11_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23824.1"
                     /translation="MDFLHERVFDPILQSRQASETLKRGVRYTIMRMNERNAAGMIQY
                     FWSAVVGTDPSVAFASRMRAEGFDRFEEVIDEFRQRFAPRPKVGRT"
     gene            6197..6421
                     /locus_tag="PLQ11_02615"
     CDS             6197..6421
                     /locus_tag="PLQ11_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23825.1"
                     /translation="MSDKVHDLTLDVLKQLQQGFAEMRERLDRIEEVARKQRRDSAGM
                     LVMMRATAGDFDERVTDVEERVLALESRKS"
     gene            6500..7567
                     /gene="fba"
                     /locus_tag="PLQ11_02620"
     CDS             6500..7567
                     /gene="fba"
                     /locus_tag="PLQ11_02620"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799936.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HRK23826.1"
                     /translation="MARITMRQLLDHAAEQGYGVPAFNINNMEQALAIMAAANEVDAP
                     VIIQASRGARQYANDIMLKHMMDAVVEIYPHIPVCVHLDHGNEPGTCMTAIQAGFTSV
                     MMDGSLKADGKTPGDWDYNVGVTKAVTQMAHLGGISVEGELGVLGSLETGEGEKEDGH
                     GAEGKLSHDQLLTNPDEAVKFVKETKVDALAIAMGTSHGAYKFSRKPDGKVLAMNVIE
                     EIHRKMPNLHLVMHGSSSVPQELQDIINAYGGKMKPTWGVPVEEIQRGIKNGVRKINI
                     DTDNRMAMTGQIRKVFAESPSEFDPRKYLKPAQEAMTALCRQRLQEFGTAGNASKIKK
                     IYTLADMAKRYAKGELDPKFS"
     gene            7701..8333
                     /locus_tag="PLQ11_02625"
     CDS             7701..8333
                     /locus_tag="PLQ11_02625"
                     /inference="COORDINATES: protein motif:HMM:NF014622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HRK23827.1"
                     /translation="MSAFLYLILPRVEDAAEFARRLAVVLAAAPVAAVLIPSAGDDKA
                     MGVIARALVPVIQQAGAAAILEQPQDARLVARAGADGAHYPHDAPGLAEAIATLKPQR
                     IVGIGGLRSRHDAMQAGERDVDYLMFGEPRADGFVPPFDQTIERAGWWADIFNTPAVA
                     CAADAAAVGPMAETGAEFVALGPWIFSQPDPAGSLGEAFRLASARRKKPD"
     gene            8338..9129
                     /locus_tag="PLQ11_02630"
     CDS             8338..9129
                     /locus_tag="PLQ11_02630"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014893042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HRK23828.1"
                     /translation="MINSPTINVMTAAALKAGRSLRNDFGRLPSLAVARKGPGDFVSQ
                     ADKKAEDILYAELEKARPGYSFRMEESGVREGVDKTHCWHVDPLDGTTNFLHGIPQFA
                     VSIGLEREGALVAGVIYNPITDDLYVAEKGQGAFHNNRRMRVSACIEPADALIAIGIP
                     HIGKAGAPQFLKELAATMARFGGHRRMGAAALDLAGVAAGQYEAFYERGLNSWDIAAG
                     IVLVREAGGFVSDLTGGNRFLETGGLVAGNEAIQRELVSVLTRLG"
     gene            9209..10186
                     /locus_tag="PLQ11_02635"
     CDS             9209..10186
                     /locus_tag="PLQ11_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotA"
                     /protein_id="HRK23829.1"
                     /translation="MTDTTDALRLSAPRIYLVRMIVFLILAGLIGLVLYKQIAVAFMA
                     NPALNGLIFGVLAIGAALAIRQVARLFGEVRWVNSIRRQEQRLTLNEPPVLLAPMAAL
                     LGEKAGREVISTLTFRSVLDSIGARLDESRDITRYLTGLLVFLGLLGTFWGLLETVGS
                     IGKVVASMPTGGDATVLFDELKAGLTAPLAGMGISFSSSLFGLAGSLILGFLDLQAGQ
                     AQNRFFYELEDWLSTMTQDRAMPAAGAQTADLKPLFDRLTHVVSEASGGRASATAMVT
                     LAEGIQTMIQHMRSEQQMIRNWVEFQADQQQEVKRLLEKLVRDEPKGHL"
     gene            10186..11208
                     /locus_tag="PLQ11_02640"
     CDS             10186..11208
                     /locus_tag="PLQ11_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan -binding protein"
                     /protein_id="HRK23830.1"
                     /translation="MPLGRARGRGGDRSISYWPGFVDALSTLLLVFTFLLAVFVLAQF
                     FLSREVSGKDEVLTRLNRQLQQLTELLSLEKGAKTSAEGQVASLTATLDLMRSENEKL
                     KGAAEGAGAAAQRAGALQGQIDSEKQLAARALAQVEQLNQQIAALRKQLQALEEALNA
                     SEAKDKDAQARISDLGNRLNVALAQRVQELNRYRSDFFGRLRQVLGARPDIRIVGDRF
                     VFQSELFFETGASDLSVAGSAELDKIAAALQDLEREIPPEIPWIARIDGHTDKRPFQS
                     GSARSNWELSANRAISVVRYLVSKGVSPQRLAATGFGEFQPIDLGESPEALARNRRLE
                     LKVTER"
     gene            11205..11669
                     /locus_tag="PLQ11_02645"
     CDS             11205..11669
                     /locus_tag="PLQ11_02645"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRK23831.1"
                     /translation="MILRDAAPADLRALIDLWQEAWQVTMPEIDFAARRPGFTGKLAD
                     MLAEGVRLRMAETDEAMAAGFYTLDAGGYLDQVAVKRSLWGRGVASRLIEDAKHLSGG
                     RLSLTVNQANHRAIAFYEREGFQRGEASVNPVSGLPLWVYRWDSGQTSGSRP"
     gene            complement(11645..12145)
                     /locus_tag="PLQ11_02650"
     CDS             complement(11645..12145)
                     /locus_tag="PLQ11_02650"
                     /inference="COORDINATES: protein motif:HMM:NF017470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HRK23832.1"
                     /translation="MPRWSRATGSMPASPHCNSAILAPDMPPLGPLLFSDQGRLSRRG
                     FWWFVALASGLALALLLLLGLRRMGLAHAVYGVAMIYPSYCVFAKRFRDIGIDGAWAM
                     LIVTISALDLVIGLGRIGARGDWLGMLATGWHYLALANVILTLVLLGGVAGDQGANRY
                     GLEPEV"
     gene            complement(12072..13865)
                     /locus_tag="PLQ11_02655"
     CDS             complement(12072..13865)
                     /locus_tag="PLQ11_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRK23833.1"
                     /translation="MRRTHTDTPADAAPRKAPLSALKGLLPFVRPYRGRILAALAALV
                     LASGATLAIPLAIRRMVDFGFKADQQGLVDAYFLMLIAVVGVLAFASGLRHYLVMTLG
                     ERIVADTRTAIFAKVMSLDGAFFDATRAGDIISRLTADTTQIKSTFGASASIALRNIL
                     LFIGAVAMMAITSPWLSGLVLGAIPLIVLPLVFAGRSVRGRARAAQDRLAEASGFAAE
                     RIGAARTVQGLNAEAGAVASFAEAANAAFDAARRSTLARSILTSIGIFLIFASVIAVL
                     WTGAQSVISGEMTPGRLSQFVLYAVFAASALGQLSDVYGDISQAAGAAASIAELLETQ
                     PQVQETPTPQLPAAPARGAIDISAVRFAYPTRAEADVFESLSLAVRPGERLAIVGPSG
                     AGKSTLFQLLMRHYDPVAGAIRIDGVPIRDMALRDLRRLIALVPQDPVIFATSVADNI
                     RLGNPAASDEAIRNAARQAAADDFIARLPQGYDTLVGERGVTLSGGERQRIAIARAIL
                     RDAPILLLDEATSALDAENERAVQTALDHAMQGRTSLVIAHRLATIRAADRIVVLDRG
                     RIAEEGDHAALVARDGLYARLAALQFGDPGA"
     gene            complement(14000..14590)
                     /gene="pcaG"
                     /locus_tag="PLQ11_02660"
     CDS             complement(14000..14590)
                     /gene="pcaG"
                     /locus_tag="PLQ11_02660"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HRK23834.1"
                     /translation="MPVERLKETPSQTAGPYLHIGMLPAQAGLKLRSQERLNVLTSAG
                     VEGERIRIEGSVYDGAGVPVRDAQIEIWQADSKGRYNAPGFLGWGRAGADFSTGTYFF
                     ETIRPGATPWPDGRMQAPHLSLLVFARGINLHLHTRLYFDDEGDANTADPLLNAIELA
                     PLRQTLVARREMRGQQIVFRFDIHLQGEKETVFLDM"
     gene            complement(14592..15317)
                     /gene="pcaH"
                     /locus_tag="PLQ11_02665"
     CDS             complement(14592..15317)
                     /gene="pcaH"
                     /locus_tag="PLQ11_02665"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007197918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HRK23835.1"
                     /translation="MASPAPFIRRDRLWHPPQFAPGYKTSVLRSPRRALLSLEAALGE
                     LSGPKFGHDMLAHDDNDLIRNHMVSGEAVGERIIVHGRVVDENARPVPGTLLEVWQAN
                     AGGKYRHVNDHYKAADDPNFGGCGRCITDADGHYYFRTIRPGPYPWRNNGSDWRPAHI
                     HVSVFGEAFAQRLVTQMYFEGDPLIPLCPIVNTIPTLAARNMLIAQLDMGAMVPFDSL
                     AYRFDIVLRGRRQAFFENRTEGL"
     gene            15538..16221
                     /locus_tag="PLQ11_02670"
     CDS             15538..16221
                     /locus_tag="PLQ11_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1013 domain-containing protein"
                     /protein_id="HRK23836.1"
                     /translation="MATLLMPKATAVWLVDNTPLTFDQIADFCRLHPLEVKGIADGEV
                     ATGIKGYDPVVAGQLTREELNRAIADPEYRMHVAEPKVRVPEVKRAKGPRYTPVSKRQ
                     DRPNAILWLLKNHPELKDAQIMRLVGTTKSTILSVKERTHWNATNLAPMDPVMLGLCS
                     QLDLDFEVQRASKERPVPVETGATLLPAAVTTAPEAEELEPQRSAAAVKKEEDDEIAS
                     VFAKLKELK"
     gene            complement(16328..17152)
                     /locus_tag="PLQ11_02675"
     CDS             complement(16328..17152)
                     /locus_tag="PLQ11_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HRK23837.1"
                     /translation="MDGQDLLAQTQTQSESSARPDPVVRTIQFSDIADALAAGMRDFR
                     AAPFYGLTFGALYALGGILIVATVAALNMPYFTYPLAIGFVLIGPLVAIGLYEISRLL
                     ERGEKITWGSIFSAIWTQAGKEIGWMCFVVLFITTMWLYQVRLLLALFLRNETMTSMG
                     EFFTVVSTTSQGWMFLGVGHVIGAILSTVMFSLTVVSFPILLDRTIDFITAMITSVRA
                     VVNNPKPMLTWAAIVVATLFVASMPMFLGLVVVLPVLGHTTWHLYRKLVEPEQPAG"
     gene            17372..19012
                     /gene="ipdC"
                     /locus_tag="PLQ11_02680"
     CDS             17372..19012
                     /gene="ipdC"
                     /locus_tag="PLQ11_02680"
                     /EC_number="4.1.1.43"
                     /EC_number="4.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate/phenylpyruvate decarboxylase"
                     /protein_id="HRK23838.1"
                     /translation="MKLAVALAEALKARGAAEIFGLPGDFALPLFRELEEAGTLPIHT
                     LSHEPGLGFAADAAARLRGTLACAVVTYGAGALNIVNAVAQAYAEKSPLVIVSGAPAK
                     AQRAMGLGLHHQVKHFDSQLAIFRELTAAQAVLDNPETAAAEIARVLDVAINLSRPVY
                     IEFPRDMVAAEVAPVPLFRPAVTDPAAAAAAADEIMARLKSAKRPALLLCVEVRRYGL
                     EAKVEELARRLAIPAATTFMARGLLPGSEAPLIGTYLGLAGRAEVREIVEGADCLLML
                     GVILCDTNFGVSERLIDMRTSIHAFDREVRFGHHVYPEIGLPALVDALLERARPLGAA
                     RRKKRVDYPHGLIEDATPLVPDDVSTAVNDLFKAVGHTMPIASDMGDCLFAAMEIVHT
                     PLVAPAYYATMGPGVPMGLGLEIAGAGRPLIMVGDGAFQMTGWELGNARRLGLKPIVL
                     LFNNTSWGMLKAFQSQAAYNDLDDWRFAEMAAPLGGVGVRVTTRAELANALREAYADK
                     GHWRMIEIMLPRGVFSKTLRRFTEVIGARAAGTPNNAK"
     gene            19094..19609
                     /locus_tag="PLQ11_02685"
     CDS             19094..19609
                     /locus_tag="PLQ11_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009341116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRK23839.1"
                     /translation="MKHGILAAGLVCLAGAAGAAEVVALGASNTEGRGRGATPDGVPQ
                     AQAFPAQLQALLRSEGCKAKVLNAGVAGDTTGGMLRRLPRVLGKDTKVLILQPGGNDA
                     RHGESGNTASNIAAMRSHAEARGVTVVMMERLQAIAGSNLLGDGVHYNAQGHAQFAAY
                     VLPEVRAAACR"
     gene            complement(19631..21541)
                     /locus_tag="PLQ11_02690"
     CDS             complement(19631..21541)
                     /locus_tag="PLQ11_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA synthetase"
                     /protein_id="HRK23840.1"
                     /translation="MAGSSKGAYSKLYQGWQKDPKSFWGEAAKAIDWYVPPKTVLDEK
                     AGVYGRWFPDGVCNTAYNCLDRHVAAGRGAQDAILYDSPVTGTKARISYAALLDEVQV
                     LGQMLRDRGVRKGDRVLLYMPMIPEAVIGMLACARIGAVHSVVFGGFAARELATRIDD
                     CKPKAILSASCGIEPGRIVKYKPLLDEAISLSSSKPETCLIFQRPQETAALVAGRDED
                     WAAARDAMRASGKAKGCAPEKMQATDPLYVLYTSGTTGKPKGVMRDTGGHMVALAWSM
                     KGLYNALPGEVFWAASDVGWVVGHSYICYAPLLVGCTTILFEGKPVGTPDPGTFWRII
                     EEYQVRNLFTAPTALRAIKKEDPEGAFIRKYNIKPLKTLFLAGERADPDTVLWAEKML
                     GVPVVDHWWQTETGWAIAGNPTGVELLPIKPGSPTVPMPGFEVHILDESGKEVEAFKM
                     GSIAIKLPMPPGALPTLWQADDRFRESYLSEFKGYYSTSDAGYRDADGYLYIMGRTDD
                     IINVAGHRLSTGGMEEVLASHPSVAECAVIGAKDTLKGETPLGFVVLKAGVTKSAVEI
                     EKECAALIREKIGPVAAYRHTVVVARLPKTRSGKILRGTMKKIADHDPWTMPATIDDP
                     AALDEIAKALKS"
     gene            21757..22032
                     /locus_tag="PLQ11_02695"
     CDS             21757..22032
                     /locus_tag="PLQ11_02695"
                     /inference="COORDINATES: protein motif:HMM:NF025122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4164 family protein"
                     /protein_id="HRK23841.1"
                     /translation="MSQEPVQEALSRFSQALDRLEAASLRHQEGERLRIALESELALM
                     RADRQSLAEALDQSVARGRQLEEQFQLIDQRIERAIASVRDCLNADG"
     gene            22036..22404
                     /locus_tag="PLQ11_02700"
     CDS             22036..22404
                     /locus_tag="PLQ11_02700"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HRK23842.1"
                     /translation="MSQVQITIDGKTFRMACEDGQEAHLEALGRHLDGRIGEMRKTFG
                     EIGDLRLVVMAAITITDEFFEARRRTETLGKEMQLVRSEQSSVAADAGQREAEIAGAI
                     DRMTQRLERITALLATRPDL"
     gene            22445..22600
                     /gene="ssrS"
                     /locus_tag="PLQ11_02705"
     ncRNA           22445..22600
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PLQ11_02705"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
BASE COUNT         4124 a         6985 c         7544 g         3980 t
ORIGIN      
        1 ttagagcatg tcttctgcga ttgaccggta tccacttaat cgcgacatgc tctaggcgat
       61 gatgtccggc gtcagccggt ccgacaggcg ggtgatctcg tccttcagca gcagcttgcg
      121 cttcttgagc cgctggatct gcaacttgtc cggcgcaccg agtgcttcca gcgccaggat
      181 tgcatcgtcg agatcccgat gttcctgcct atgctgctcg agttcatggg tcagagcctg
      241 ccgctcggct tcactcatcg gctgacgcat cgggcctacc cttcacaaat tccaaagtcg
      301 cgcggcgccg gatggtcctg caaccgcaaa caatgttcgg ccggagcgag tatggtccgg
      361 cttgcgccgg ccttcaagtc gctgttgaca ggttgctaac acttagccgc cgaaccccct
      421 tgcggacgcc tgtggctgtg gcatgctgct tacgtcgttc aacgcgcaca ggagccacga
      481 atgtcggtgg aatcacatct cgcccagttg gaacgcaaac atcaggcgct caaggatgcg
      541 atcgcagcgg aactcgcgca tcccgcgtgt gatgatgcca aggtcgcggc gatgaagcgc
      601 aagaaattgc tgctcaagga tgaaatcgcc aagctcaagc cgcaggttca ggcgccctcc
      661 gcgctgcatt gagcgattcc ccccgcgtca gccgggcgcg atggcgcctg gcgtgcctgc
      721 tatcaggcgt gatgcgtgcc aaggcgggcg gtcggcccgc cttttccatg acgaaacatg
      781 tgctttttgc agcatttcag gtctgatccg gcaaaatccg ctggtatttg atccacattc
      841 gttgacaggg cgccgcgatg ggctatcgag gcgcagcagg acgagcgact gatcgcggcg
      901 tcccttccca gtctccgcgt ttttcaggat cttcgtatgc cgacccgcgc ccagcatgat
      961 ttgatggcca atgccgttcg cgcccttgcg atggatgcgg tggaagccgc caagtcgggc
     1021 catccgggcc tgccgatggg ggcagccgac gttgccaccg tcctgttccg cgaccatctc
     1081 aagttcgacc ctgccgatcc ggcctggccg gatcgtgacc gtttcgtgct ctcggccggc
     1141 catgggtcga tgctgatcta tgctctgctg catctgatcg gtgtcaagca ggccagtctt
     1201 gatgagttga aggcgtttcg ccagcttggc tcaaagaccg caggacatcc cgaatacggc
     1261 catcttgccg gcgtcgagac gacaaccggg ccgctggggc agggcatcgc caatgcggtc
     1321 ggcatggccc tggccgagcg gctgatggcg gcacgccatg gcggcaagct cgtcgatcac
     1381 aatacctatg tgctggcggg tgacggctgc ctgatggagg ggatcagcca ggaggcgatc
     1441 gcgctcgccg ggcatctgaa gctcaaccgg ctgatcgtct tgtgggatga caacaacatc
     1501 accatcgacg gcaaggtgtc gctggctgat tcgaccgatc aactgaagcg gttcgaggcg
     1561 tcgggctggc gcgctgaacg ggtcgacggt catgatccgg tggcgatttc cgccgcgctc
     1621 gaccgggcca aggcagcgga caggccgtcg ctgatcgcct gcaagacggt gatcggatac
     1681 ggtgcgccga ccaaggcggg cagcgaaaag gtgcatggtg ccccgctcgg gaccgatgaa
     1741 ctggccgcga ccaagaaggc gctcggctgg ccgcatggcg ctttcgtcgt tccgccggag
     1801 gtctatgctc tgtggcaggc gacggccaag cgcagcgctg gcgcccgcag ggcctggacg
     1861 cagcggctgg aggcgaccgg cgccaagaaa cgcgccgcct tcgaacaggc gcttgcaggc
     1921 acccagccca aggaactggc gcgtgcgctc aagggcttga agcagcaggc ggctgtgaca
     1981 accgccgaga tcgcgacgcg caagacgtcg gaaatgacca tcgacgccat cgccggggcc
     2041 atggatgcgt tcgtctccgg ttcggccgac ctgacccatt ccaatttcac gcaaggcaag
     2101 ctgctgaagc cggtcaagcc tggcgatttt tccggcggct acatccatta cggcattcgc
     2161 gaacatgcga tggccgccgc aatgaacggc atcgccctgc acggcgggct gatcccggca
     2221 ggcggcacgt tcttcgtgtt ttccgactat tgccgcccgt cgatccggct ggccgcgctg
     2281 atgggcctgc gcgtgctgca tgtgatgacg catgattcaa tcggcgtcgg cgaggatggg
     2341 ccgacgcatc agccggtcga gcatctggcg tcgctgcgcg ccattcccaa ttgcacggtg
     2401 ctgcgcccgt gcgacctgac cgagacggtc gaggcctatg aagtggcgct cgtcgctgcc
     2461 aggacgccga cggtgttcgc gctgtcgcgg cagaacctgc cgcagctgcg caagtcttac
     2521 acgccgaaga acctgacagc gctcggcgcc tatgaactga tgccggcgga aggcaaggcg
     2581 caggtgtcct tgttcgcttc ggggtcggag gtggcgatgg ccatcgaggc gcgccgcctg
     2641 ctggcggaaa aggggattgc cgcgcgggtg gtgtcggttc ccagccacga tctgttcgcc
     2701 gcccagccgg aagcctatcg ccgcaaggtg atcggccggg cgaaggtgaa ggtcggcatc
     2761 gaggccggca tccggcaagg gtgggatttt atcattggca gcgacggatt gttcatcggc
     2821 atgaacggtt tcggcgcttc cgggccggcg gcaaaggttt atcagcattt tggcattacc
     2881 gcgcaggcgg tggctgacgc tgcggcacaa cgcctggcgc gttgaggttg gcttttattc
     2941 agaccgggct gtgcatgagg cgcggcatcg aacgttcaag aggagcacat catggctgtg
     3001 aaggttttca tcaacgggtt cggtcgtatc ggccgcaacg tcctgcgcgc gatcatcgag
     3061 ggcaagcgca aggacatcga ggtggtgggc atcaacgatc tcggcccggt cgaaaccaat
     3121 gcccatctgc tgcgctatga ttcggtgcat ggccgctttc cggccgatgt gaaggtcgag
     3181 ggcgagagcc tggtcatcaa cggccagaag atccgcgtca ccgcgatcaa ggatccggcg
     3241 agcctgccgc acaaggaact gggcgtcgac atcgcgctcg agtgcaccgg catcttcacc
     3301 gccaaggaca aggccagcgc ccatctcaag gccggcgcca agcgcgttct ggtctcggcc
     3361 ccagcggaca atgccgatta caccgttgtc tacggcgtca atgacgccgg cctgaccaag
     3421 gatcacctgg tcgtctcgaa cggctcctgc accaccaact gccttgcgcc ggtcgccaag
     3481 gtgctgaacg actcgttcgg catcgtgcat ggcttcatga ccacggtgca tgcctacacc
     3541 ggcgatcagc cgacgctcga caccatgcac aaggaccttt accgcgcccg cgctgcggca
     3601 ctgtcgatga tcccgacctc gaccggtgcg gcgaaagcca tcggcctggt catcccggaa
     3661 ctgaagggca agctcgacgg ctccgccatc cgcgttccga ccccgaatgt ctcggtggtc
     3721 gatctgaagt ttgtctcgaa gaagaaggtc acggtcgaga agatcaacga cgcgatcctc
     3781 aaggcggcca agcgcggccc gctcaagggc atcctcgcgg tgacggccga gccgaatgtc
     3841 tcgtccgact tcaaccacgc gccggcctcc tcgaccttcg cgctcgacca gaccaaggtg
     3901 atggacgaga agttcgtccg catcctcacc tggtatgaca acgaatgggg cttctccaac
     3961 cgcatggtgg acagcgcggt ggcgatgggc aagctgatct gaggcaacag gggccgctcc
     4021 gcacccggag cggccccttc cgcactgctc caacgatgaa ccggcgttca ttcccgttcc
     4081 tgcttcccgc caccgccgcc atgcttgcga tggtgctggc cgggccggct gtggctgtta
     4141 ccgatgcggc gaccgggttt ggcatcgcac cgccggcgcc gttcaccgcc gagcggagcg
     4201 ccgccaatgc cgaggatgtc gccttctcga tccgctcgcc ggacggacaa ccgccggcgg
     4261 ccggcaccgg tgcgctctgc cgcgccggtt tcaaggccgc agccacctcc agcggtgcag
     4321 accagccgac gctcaatgcc aaactcgaca ggccgccggt gcttgatgag atgcgcaagc
     4381 cgctcaagcc gcgctacgtg atcgaacgcg aggatctgtt ccagctcggc gaggcgcgcg
     4441 gcattgccta taccgctatt ccccgcgccg gaccgaatgc cgatacggtg cgcgtggtgc
     4501 tgtcgtttgt cgaaacgccc aaggggcggg tgagcctcgt ctgcgccacg cccgctcccc
     4561 atcttgaagc ggcccgcccg gtgttcgagg cgatccgcgc cacgcttatc ctgccgaaat
     4621 aggattttgt catgagccag actttccgca ccctcgacgc gctcgctccc aagggcaagc
     4681 gcgtgctcgt ccgtgtcgat ctcaacgtgc cgaccgagaa cggcagggtg accgacgcaa
     4741 cccgtcttga tcggatcctg ccgaccatca acgatatcgc cggcaagggg gggcgggtga
     4801 tcctgctggc gcatttcggc cggccgaagg gcagggacgc ttcgcaaagc ctcaagccgg
     4861 tggccgagga acttgcccgc cgtctcggca agccggtggc cttcgccgag gattgcatcg
     4921 gcgatgtcgc ggccagggct gtcgcggcga tgaaggacgg cgatgtgctc tgtctggaaa
     4981 acacccggtt ctacaaggaa gaggagaaga acgagccggg ctttgttgcc gaactcgcca
     5041 ggctcggcga tgcctatgtc aacgatgcat tctctgccgc ccatcgcgcc catgcctcga
     5101 ccgaggggct ggcgcacaag ctgccggcct atgccggccg gacgatggaa gcggaactga
     5161 aggcgctcga tgcggcgctc ggcaatccga agaagccggt ggtggcgatt gtcggcggcg
     5221 ccaaggtctc gaccaagctc gatctgctcg gcaatctggt gaagaaggtc gatatcctga
     5281 tcatcggcgg tggcatggcc aacacattcc tcgcagcgcg cggggtcaat gtcggcaaga
     5341 gcctgtgcga gcacgatctg gtcgccaccg cgcgcgagat cgagcagaag gccaaggcgg
     5401 cgggctgcga gatcgtgctg ccggtcgatg cgctgctcgc caaggagttc aaggccaatc
     5461 cgggccatcg cgcggcgggt gttgccgatg tcaaggctga tgagatgatc ctcgatgccg
     5521 ggccgaagtc ggtcgcggta gtcgaggcca agctgaaatc ggccaagacg ctggtgtgga
     5581 acggcccgtt cggcgcgttc gaactcgctc cgttcgacac ggcgaccgtg gcggccgcga
     5641 aaaccgccgc tgcgctgacg aaagccggaa gccttgtctc catcgccggc ggcggcgaca
     5701 ccgtggcggc gatgaaccac gccggcgtcg ccgacgacct gacctatatc tcgaccgctg
     5761 gcggcgcctt cctcgaatgg atggaaggca aggctctgcc gggggttgaa gcgctgaagc
     5821 ggtaagaggt cagcaatgca tccggtcggc tctcaggaaa gacagatcat ggattttctg
     5881 catgagcgtg tattcgatcc gattttgcaa tcccgccagg cctctgaaac gttgaagcgg
     5941 ggcgttcggt acacgattat gcgcatgaat gagcgaaacg ccgctggaat gattcaatat
     6001 ttctggtcag cagttgtcgg gaccgacccg agtgtcgcgt ttgcatcaag gatgagggcc
     6061 gagggtttcg accgtttcga agaagtcatc gacgaattca ggcagcgctt cgcgccgcgc
     6121 cccaaggttg gccgaacatg atcctcggca agtgcctcac gttttcatgt caccagaaac
     6181 atgttagagt ttgctcatga gcgacaaagt gcacgatctt actctggacg ttctcaagca
     6241 gctgcaacaa ggcttcgcag aaatgcgtga gcgccttgac cggattgagg aagttgcgcg
     6301 caagcagcgg cgcgattccg ccggtatgct tgtcatgatg cgggcgaccg ccggggattt
     6361 cgacgagcgc gtgacggatg tcgaagagcg cgttctcgcc cttgaatccc gcaaatcctg
     6421 acacaccgct ttagttccag ccggccgcag ggtcggcttt cgcacacctc agccctacac
     6481 ccaacggaga acgaccccca tggcccgcat cacgatgcgt caacttctcg atcacgccgc
     6541 cgagcaaggc tatggcgtgc ccgccttcaa catcaacaac atggagcagg cgctggcgat
     6601 catggcagcg gccaacgagg tcgatgcgcc ggtgatcatc caggccagcc gcggcgcccg
     6661 tcaatatgcc aacgacatca tgctcaagca catgatggat gcggtcgttg aaatctatcc
     6721 gcacatcccg gtctgcgtgc atctcgacca tggcaacgag ccgggcacct gcatgaccgc
     6781 aatccaggcc ggcttcacct cggtgatgat ggacggctcg ctcaaggcgg acggcaagac
     6841 cccgggcgac tgggattaca acgttggcgt taccaaggcc gtgacccaga tggcgcatct
     6901 cggcggcatc tcggtggaag gcgagctggg cgtgctcggc agcctggaga ccggcgaggg
     6961 cgagaaggag gacggccacg gcgccgaggg caagctcagc cacgaccagc tgctgaccaa
     7021 ccccgacgag gcggtgaaat tcgtcaagga gaccaaggtc gatgcgctgg cgatcgccat
     7081 gggcaccagc cacggcgcct acaagttctc gcgcaagccg gacggcaagg tgctggcgat
     7141 gaacgtgatc gaggaaatcc atcgcaagat gccgaacctg catctggtga tgcacggctc
     7201 ctcgtcggtt ccgcaggaac tgcaggacat catcaacgcc tacggcggca agatgaagcc
     7261 gacctggggc gtgccggtgg aggaaatcca gcgcggcatc aagaacggcg tgcgcaagat
     7321 caacatcgac accgacaacc gcatggcgat gaccggccag atccgcaagg tgttcgccga
     7381 gtcgccgtcg gagtttgacc cgcgcaagta tctcaagccg gcgcaggagg cgatgaccgc
     7441 gctgtgcagg cagcgcctgc aggagttcgg caccgccggc aacgcctcga aaatcaagaa
     7501 gatctacacc ctcgccgata tggccaagcg ttatgccaag ggcgagctgg atccgaagtt
     7561 ctcgtaagac cgggttctga tcaccgcagg atggccgggg ttcgccccgg ccatttccgt
     7621 ttttgctgcc ggtgttttgc ctgccgatgt ttttcttgcc tgcttgccgc atggcagcgg
     7681 ggcgcgtaag aagcgcggcc atgtccgcct ttctttatct catcctgccg cgggtcgagg
     7741 atgccgctga attcgcgcgc cgcctggctg tagtgctggc ggcggcgccg gtcgcggcgg
     7801 tgctgatccc gtcggccggt gatgacaagg caatgggcgt gattgcccgg gcgctggtgc
     7861 cggtgatcca gcaggcaggt gcggcggcca tcctcgaaca gccgcaggat gcgcggctgg
     7921 ttgcccgcgc cggggccgat ggtgcgcact acccgcatga cgcaccggga ctggccgagg
     7981 cgatcgcgac gctgaaaccg cagcggattg ttggtatcgg gggccttcgg agccggcatg
     8041 acgccatgca ggcaggcgag cgcgatgtcg attatctgat gttcggcgaa ccgcgcgccg
     8101 acggatttgt tccacctttt gaccagacga tcgagcgtgc cggctggtgg gcggacattt
     8161 tcaatacgcc tgcggttgcc tgtgcggccg atgccgctgc tgtcgggccg atggccgaaa
     8221 ccggcgcaga attcgtcgca ctcggcccat ggattttctc gcaacccgat cctgccggtt
     8281 cgctcggtga ggcattccgc ctggccagcg cgcggcgaaa gaagccagac tgacatcatg
     8341 atcaattccc ccactatcaa tgtgatgacc gctgccgcgc tcaaggccgg acgttcgctg
     8401 cgcaacgatt tcggccggct gccgtcgctc gccgtcgccc ggaagggccc cggcgatttt
     8461 gtttcccagg ccgacaagaa agccgaggac atcctctacg ccgagttgga gaaggcgcgg
     8521 ccgggctatt ccttccgcat ggaggaatcg ggcgtgcgcg agggggtgga caagacccat
     8581 tgctggcatg tcgatccgct cgacgggaca acgaatttcc tgcacggaat cccgcagttt
     8641 gccgtctcca tcgggctgga acgcgagggc gcattggtcg ccggggtcat ctacaatccc
     8701 atcaccgacg atctctatgt cgcggaaaag ggccagggcg ccttccacaa caaccggcgg
     8761 atgcgggttt ctgcctgtat cgagccggcg gatgcgctga tcgccatcgg cattccacac
     8821 atcggcaagg cgggtgcgcc gcagttcctg aaagagctgg cggcgacgat ggccagattc
     8881 ggcggccatc gccgcatggg ggcggcggcg ctggatctcg ccggggttgc cgccggccag
     8941 tatgaggcct tctacgagcg cggcctcaac agctgggata tcgccgccgg catcgtgctg
     9001 gtgcgtgagg caggcgggtt tgtttcggac ctgacaggcg ggaacaggtt cctggaaacc
     9061 ggaggcctgg tcgccggcaa tgaagcgatc cagcgcgagt tggtgtcggt gctgaccagg
     9121 ctgggctgag cctgcggcgc cagagccgct tgaaattgcc gcaatgctgg cccactctgc
     9181 cggccgtcgc gattgtaagc gaggccgcat gaccgatacg accgatgccc tgcgcttgtc
     9241 tgcgccgcga atttatctgg tgcggatgat cgtctttctc attctggccg ggttgatcgg
     9301 tctggtgctc tacaaacaga tcgctgtcgc gttcatggcc aatccggcgc tcaacgggct
     9361 gattttcggc gtgctggcca tcggtgcagc gctggcgatc cggcaggtgg cccgcctgtt
     9421 cggcgaagtg cgatgggtca attcgatccg gcggcaggaa cagcggctga cgctcaacga
     9481 accgccggtg ctgctggcgc cgatggctgc attgctgggt gaaaaggccg gtcgcgaggt
     9541 catatcgacg ctgacattcc gctcggtgct cgattcgatc ggtgcgcgcc tcgacgaatc
     9601 gcgtgacatc acgcgctatc tgaccgggct gctggtgttt ctgggcctgc tcggcacgtt
     9661 ctggggattg ctggaaacgg tcggctcgat cggcaaggtt gtggcctcga tgccgaccgg
     9721 cggcgatgcc accgtgctgt ttgacgaatt gaaggccggt ctgacggcgc cactcgccgg
     9781 catgggcatc tcgttctcgt cctcgctgtt cggtctcgcc ggctcgctta tcctcggttt
     9841 tctcgatctg caggcggggc aggcgcagaa ccggtttttc tacgagttgg aagactggct
     9901 ctcgacgatg acgcaggatc gcgcaatgcc ggccgccggt gcgcagaccg ccgacctcaa
     9961 gccgctgttc gaccggctga cccatgtggt gagcgaggcc agcggcgggc gtgcatcggc
    10021 cacggcgatg gtgacgctgg ccgaaggcat ccagacgatg atccagcata tgcgctccga
    10081 acagcagatg attcgcaact gggtggagtt ccaggcggac cagcagcagg aggtcaaacg
    10141 cctgctggaa aagttggtgc gcgatgagcc gaaaggccac ctctgatgcc gctcgggcgg
    10201 gcacgcgggc gcggggggga ccggtcgatc tcctattggc ccggctttgt cgatgccctg
    10261 tcgacgctgc tgctggtgtt caccttcctt ctggccgtgt tcgtgctggc gcaattcttc
    10321 ctgtcgcgcg aggtcagtgg caaggatgag gtgctaaccc ggcttaaccg ccagcttcag
    10381 caattgaccg aattgctcag cctcgaaaag ggcgccaaga cctcggcgga aggccaggtt
    10441 gccagcctga cggccacgct cgatctgatg cgaagcgaga acgagaagct gaagggcgcg
    10501 gccgaggggg ccggtgccgc ggcccagcgg gccggggcgt tgcagggcca gatcgacagc
    10561 gagaagcaat tggcagcgcg ggcgctggca caggtcgagc agctcaacca gcagatcgcg
    10621 gccttgcgca agcagttgca ggcgctggaa gaggccctca acgcctccga ggcgaaggac
    10681 aaggatgcac aggcgcgcat ttccgatctt ggcaaccggc tgaatgttgc gctcgcccag
    10741 cgggtgcagg agctgaaccg ctaccgatcc gatttcttcg gccggctcag acaggtgctt
    10801 ggtgctcgtc cggatatccg catcgtcggc gatcgtttcg ttttccagtc ggagctgttc
    10861 ttcgagaccg gggcgtccga cctttcggtg gccggttcgg ccgaactcga caagatcgcc
    10921 gccgctctgc aggatctcga gcgtgagatt ccgccggaaa taccgtggat cgcccggatc
    10981 gacggtcata ccgacaagcg gccattccag tcaggatcgg cgcgttccaa ctgggaactc
    11041 tcagccaatc gggccatttc cgtcgtccgc tatctggtca gcaagggcgt atcgccacag
    11101 cggctggcag ccaccggctt cggcgaattc cagccgatcg accttggcga aagccccgag
    11161 gcactggcgc gcaaccggcg gctcgaactg aaggtcaccg aacggtgatc ctgcgcgatg
    11221 ccgcgccggc cgacctgcgg gcgctgatcg atctgtggca ggaggcctgg caggtcacca
    11281 tgccggagat cgactttgcc gcgcgtcggc cgggctttac cggcaagctg gcggatatgt
    11341 tggccgaggg cgtgcggttg cgcatggcgg agacagatga agcgatggcc gccggattct
    11401 ataccctcga cgctggcggc tatctcgacc aggtggcggt gaagcgcagt ctttggggac
    11461 gcggggtggc cagccggctg atcgaggatg caaaacacct gtccggcggg cggctgtcgc
    11521 tcacggtcaa tcaggccaac caccgcgcca tcgccttcta tgaacgcgag gggttccagc
    11581 gcggcgaagc ctcggtcaat ccggtgtccg gtctgccgct ctgggtgtac cggtgggaca
    11641 gcggtcagac ctcgggctcc aggccgtagc ggttcgcccc ctgatcaccg gccacaccgc
    11701 ccagcaggac cagcgtcagg atcacattgg ccagggcgag atagtgccag cctgttgcca
    11761 gcatgcccag ccaatcgccg cgcgcgccga tccggccaag tccgatgacg agatccagcg
    11821 cagaaatcgt gacgatcagc atggcccagg cgccatcaat gccgatatcg cggaagcgtt
    11881 tggcaaagac gcaatagctc ggataaatca tggcgacgcc atagacggcg tgggccaggc
    11941 ccatgcggcg tagcccaagc agcagcagca gggccaatgc caggccggag gccagtgcga
    12001 cgaaccacca gaaaccgcga cgcgacagac gaccctgatc ggagaacagc agcgggccga
    12061 gcggcggcat gtcaggcgcc aggatcgccg aattgcaatg cggcgaggcg ggcatagagc
    12121 ccgtcgcgcg cgaccagcgc ggcatgatcg ccctcttcgg cgatccggcc ccgatcgagc
    12181 acgacgatgc ggtcggccgc gcggatagtt gccaggcgat gggcgatgac cagcgaggtg
    12241 cgcccctgca tggcatggtc gagggcagtc tgcaccgctc gctcgttctc ggcatcgagg
    12301 gcggaggttg cctcgtcgag caggaggatc ggcgcatcgc gcagaatggc gcgggcaatg
    12361 gcgatgcgct ggcgttcgcc gcccgaaagg gtcacgccgc gttcgccgac cagcgtgtca
    12421 tagccttgcg gcagccgggc gatgaaatca tcggcggcgg cctgccgggc ggcattgcgg
    12481 atggcctcgt cgctggccgc cggattgccg aggcgaatgt tgtcggccac cgatgtcgcg
    12541 aagatgaccg ggtcctgcgg caccaacgcg atcaggcggc gcagatcacg caaggccatg
    12601 tcgcggatgg gcacgccgtc gatccggatc gcgccggcaa cgggatcgta gtgccgcatc
    12661 agcagctgga acagggtcga cttgcctgcg cccgaggggc cgacaatcgc cagccgctcg
    12721 ccgggacgca cggccagcga gagcgattcg aacacatcgg cttcggcgcg cgtcggatag
    12781 gcaaagcgca ctgcggaaat gtcgatggcg ccccgggccg gtgcggcggg cagctgtggc
    12841 gtcggtgtct cctgcacctg cggctgggtc tccagcaatt cagcgatcga ggctgccgct
    12901 ccggcggcct ggctgatgtc gccgtaaaca tcggacaact ggcccaatgc gctggcggca
    12961 aagaccgcat agagcacgaa ttgcgacagc cggcccggtg tcatttcgcc ggaaatcacc
    13021 gattgggcgc ccgtccacag gactgcgatc accgaggcaa agatcaggaa aatcccgatc
    13081 gaggtgagga tcgaacgggc cagggtcgag cgccgggccg catcaaaggc agcgttggcg
    13141 gcctcggcaa agcttgcaac agcgccagcc tcggcattga gaccctgcac agtgcgggcc
    13201 gcgccgatcc gttcggcggc aaaaccggag gcttcggcga gcctatcctg cgccgcgcgt
    13261 gcgcggccgc gcacggagcg gccggcaaag accagcggca gaacgatcag cgggatggcc
    13321 cccagcacca ggcccgacag ccaggggctg gtaattgcca tcatcgccac cgcgccgatg
    13381 aacagcagaa tgttgcgcag ggcaatcgag gcagaggccc cgaaggtcga tttgatctga
    13441 gtggtgtcgg cggtgaggcg cgagatgatg tcaccggcgc gagtggcatc gaaaaaagcg
    13501 ccgtcgaggc tcatcacctt ggcgaagatc gcggtgcggg tgtcggcaac gatgcgttcg
    13561 cccagcgtca tcaccagata atggcgcagg ccggaggcga aggcgagcac ccccaccacc
    13621 gcgatcagca tcaggaaata ggcatcgacc aggccctgct ggtcggcctt gaaaccgaaa
    13681 tcgaccatgc ggcggatggc gagcgggatg gccagcgtgg cgcccgacgc cagaaccagc
    13741 gccgcgagcg ccgcgagaat ccggccgcga tagggacgga cgaagggcag cagccccttc
    13801 agggcagaga gcggcgcctt gcgcggcgcg gcatcggcgg gggtgtcggt gtgagttctg
    13861 cgcatacaag gcccggttgt tcggcttctc gctagatcat gcgacgagaa agtggaaacg
    13921 gtttttcgca ggcatcgcgc tccgggcctt ggtccggccg gatggcggag ctggcctgcg
    13981 tgaggaacat tgccacagct cacatgtcga ggaacaccgt ttccttctca ccctgtagat
    14041 ggatatcaaa gcggaacacg atctgctggc cgcgcatttc ccgccgcgcc accagcgtct
    14101 ggcgcaacgg cgccagttcg atcgcgttca acagcgggtc agcggtgttg gcgtcgcctt
    14161 cgtcgtcgaa atacagccgg gtgtgcaggt ggaggttgat gccgcgggca aagaccagca
    14221 gcgacagatg cggcgcctgc atccggccgt ccggccaggg ggtggcgccc ggccggatcg
    14281 tctcgaaaaa gtaggtgccg gtagaaaaat cagcgccggc gcggccccag ccaagaaagc
    14341 cgggggcatt gtagcgcccc ttggaatcgg cctgccagat ttcgatctga gcatcgcgca
    14401 ccggcacccc ggctccatca tagacactgc cttcgatgcg gatgcgctct ccctcgacgc
    14461 cagcggaggt caatacattg agccgttcct gactgcgcag tttcaggccg gcctgcgccg
    14521 gcagcatgcc gatgtgcaga taggggccgg cggtctgcga cggggtctcc ttcaaccgtt
    14581 ccaccggcat gtcacaggcc ctccgtgcgg ttttcgaaga aagcctggcg acggccgcgc
    14641 agcacgatgt cgaatcggta ggcgaggctg tcaaagggga ccatggcgcc catatcgagc
    14701 tgggcgatga gcatgttgcg cgcggccagc gtggggatcg tgttgacgat cgggcagagc
    14761 ggaatgagcg gatcgccctc gaaatacatc tgggtcacca ggcgctgggc aaaggcctcg
    14821 ccgaagaccg agacatggat atgggcgggg cgccagtcgg agccgttgtt gcgccagggg
    14881 taggggccgg ggcgaattgt gcggaaatag taatggccgt cagcgtcggt gatgcagcgg
    14941 ccgcagccgc cgaaatttgg atcatccgct gccttgtagt gatcgttgac gtggcgatat
    15001 ttgccaccgg cattggcctg ccagacttcc agcagcgtgc cgggaaccgg gcgggcgttc
    15061 tcatcgacaa cgcggccatg gacgatgatg cgctcgccta ccgcctcgcc gctcaccata
    15121 tggttgcgga tcagatcgtt gtcgtcatgg gccagcatgt catggccgaa cttgggcccg
    15181 gaaagttcgc cgagcgcggc ttccagcgac agcagtgccc ggcgcggaga gcgcaacacg
    15241 gaggtcttgt atccgggcgc aaattgcggc gggtgccaaa ggcggtcccg gcggatgaag
    15301 ggggcaggcg aggccattgg cgcatgtcca ttgcttgatg tctgcgcatc gaaccaagaa
    15361 tgcagccgtt tgtcactatc ctgcttggcg gcgccaacgg gcgttgccag cttcggcgca
    15421 tcaggttata aggcacccct tcccgtcaca tgtgatgtcc ttctgttctg ggccggggcc
    15481 gaggatgatg gactttgccg taaaggggcc cgagggctcc ttcatccgga ggtagaaatg
    15541 gccacgcttc tgatgcccaa ggcgaccgcc gtctggctgg tggacaacac cccgctgacc
    15601 tttgaccaga ttgctgactt ctgccgcctg catcccttgg aagtgaaggg catcgccgat
    15661 ggcgaggtgg ctaccggcat caagggctac gatccggtcg ttgccggcca gttgacccgc
    15721 gaggaactca accgcgccat cgccgatccc gaataccgca tgcatgtcgc cgagccgaag
    15781 gtgcgggtgc cggaagtcaa gcgcgccaag gggccgcgct ataccccggt gtccaagcgc
    15841 caggatcgac ccaacgccat cctgtggctg ctcaagaacc atcccgaact caaggatgcg
    15901 cagatcatgc ggctggtcgg caccaccaag tcgaccattc tgtcggtgaa ggagcggacc
    15961 cactggaatg caaccaatct tgcgccgatg gatccggtga tgctgggcct gtgctcgcag
    16021 ctcgacctcg atttcgaggt gcagcgcgcc tccaaggagc ggcctgttcc ggtcgagacc
    16081 ggcgcaaccc tgctgccggc cgcagtgacc actgcgccgg aagccgagga actggagccg
    16141 cagcgttccg ccgctgccgt caagaaggaa gaggacgacg agattgcctc ggtctttgcc
    16201 aagctcaagg aattgaaata actctcggac gtatggtggt tcgaacacga gaaacccggc
    16261 cgcaaggccg ggtttttcct gtttcagtgg ccgttctggg gttgcccttg cccgccgggg
    16321 tgattgctca tccggcaggc tgttccggct cgaccagctt gcgatagaga tgccatgtcg
    16381 tatggccgag caccggcagg acaaccacaa ggccgaggaa catcggcatc gaggcgacaa
    16441 acagggttgc cacgacaatc gccgcccagg tcagcatcgg ctttggatta ttcaccacgg
    16501 cccgcacaga cgtgatcatc gcggtgatga aatcgatggt gcggtcaagc aggatcggga
    16561 atgacacgac cgtcagcgag aacatcaccg tcgacaggat cgcgccgatc acatggccga
    16621 cccccaggaa catccagccc tgcgatgtgg tggagaccac ggtgaaaaac tctcccatcg
    16681 aggtcatggt ctcgttgcgc aggaacagcg ccagcagcag gcgcacctga tagagccaca
    16741 tcgtcgtgat gaacaggacg acaaagcaca tccagccgat ttccttgccg gcctgggtcc
    16801 agatggctga gaaaatgctg ccccaggtga tcttctcgcc gcgctccagc aggcggctga
    16861 tctcgtacag accaatggca accagcgggc cgatcagcac gaagccgatc gccagcggat
    16921 aggtgaaata cggcatgttg agggctgcga ccgtcgccac gatcaggatg ccgccgagag
    16981 cgtagagtgc gccaaaggtc aggccataga acggcgcagc gcgaaaatcc cgcattccgg
    17041 cggccaaggc atcagcaatg tcggagaact ggatggtgcg cactacggga tcaggccgcg
    17101 cagacgattc agattgcgtc tgcgtctggg ccaacaggtc ctgaccgtcc atcgaattct
    17161 ctccctgatc tttgctccgg gcacagaatg ccggaggctg tcgttgcctt atgaaaagcc
    17221 aacgtttgat ctaggtcaaa cggtttccat cggccaacat gggcggtcgt ggctggtttt
    17281 gcccaggttt ctgacagcct ggcgcgatat tgtgtcgcag atgttttcaa tctgctgtaa
    17341 tagtaacgaa cgttaccaaa tggggtgtgt catgaaactt gctgttgccc ttgcagaagc
    17401 cctgaaggcg cggggcgctg ccgaaatttt cggattgccc ggcgattttg cgctgccgtt
    17461 gttccgtgag ctcgaggagg caggaacgct gccgatccac accttgtcgc atgagccggg
    17521 gctgggattt gcggcggatg cggcggcgcg gttgcgcgga acgctggcct gcgcggtcgt
    17581 cacctatggg gccggtgcgc tcaacatcgt caatgccgtc gcccaggcct acgcggaaaa
    17641 gtcgcctctg gtgatcgttt ccggtgcgcc ggccaaggca caacgggcga tggggctggg
    17701 gctgcatcat caggtcaagc atttcgacag ccagttggcg atttttcgtg agctgacggc
    17761 tgcccaggcc gtgctcgaca atccggagac ggctgcggcg gaaatcgccc gggtgctgga
    17821 tgtggcgatc aacctgtcgc gcccggtcta tatcgagttt ccgcgcgata tggtggcagc
    17881 ggaagtggcg ccggtgccgt tattccggcc ggcagtgacg gatccggcgg ctgccgcagc
    17941 cgcagccgat gaaatcatgg cgcggttgaa gagcgccaaa aggccggcgt tgctgctgtg
    18001 tgtcgaggtg cggcgctatg gtctggaggc caaggttgag gaactggcgc ggcggctggc
    18061 gattccggct gcgaccacct tcatggcgcg ggggctgctg cctggctcgg aagcgccgct
    18121 gatcggcacc tatctcggtc tcgccggccg cgccgaggtg cgcgagatcg tcgagggggc
    18181 cgactgtctg ctgatgctcg gagtgatcct gtgcgacacc aatttcggcg tgtcggagcg
    18241 gctgatcgac atgcggacat cgatccatgc cttcgaccgg gaggtgcgtt tcggccacca
    18301 tgtctatccg gagatcgggc tgccggcact ggtcgatgcg ctgctcgaac gggcccggcc
    18361 gctcggcgct gcccgtcgca agaagcgcgt cgattatccg cacgggctga tcgaggatgc
    18421 gacgccgctg gtgccggatg acgtctctac cgcggtcaat gacctgttca aggcggttgg
    18481 ccataccatg ccgattgcat ccgacatggg cgattgtctg ttcgctgcga tggagatcgt
    18541 tcatacgccg ctggtggcgc cggcctatta cgccacgatg gggccgggcg taccgatggg
    18601 gctggggctg gaaatcgccg gggccgggcg gccgctgatc atggtcggcg atggcgcctt
    18661 tcagatgacc ggatgggaac tcggcaatgc ccgccggctc gggctgaaac cgatcgtgct
    18721 gctgttcaac aatacctcct ggggtatgct gaaggcgttc cagtcgcagg ctgcctacaa
    18781 tgatctggac gactggcggt tcgccgagat ggcggcaccg ctcggcggcg tcggggtacg
    18841 ggtgacgacg cgtgcggaac tggcaaacgc cttgcgcgaa gcgtatgccg acaaggggca
    18901 ttggcggatg atcgagatca tgctgccgcg cggcgtattc tcaaagaccc tgcgccgctt
    18961 caccgaggtg atcggggcgc gggcggcagg gacgccgaat aatgccaaat agtgcaagaa
    19021 tgtgattggc agtgtggcgc gtccgttgcc acaatctgcc ttggccaatt cacatggcca
    19081 gggggaaatt gccatgaaac atgggatcct tgccgcaggt ctcgtctgtc ttgcgggcgc
    19141 ggccggtgca gccgaagtgg tcgcgctcgg cgccagcaat accgagggac gggggcgcgg
    19201 ggctacgccc gacggggtgc cgcaagcaca ggcctttccg gcgcaacttc aggccctgct
    19261 gcgcagcgaa gggtgcaagg ccaaggtgct caatgcgggt gttgcgggag atacgaccgg
    19321 cggcatgctg cggcggctgc cgcgggttct gggcaaggat accaaggtgc tgatcctcca
    19381 gcccggcggc aatgatgccc gccacgggga aagcggcaac accgccagca acatcgctgc
    19441 catgcgcagc catgccgagg cgcgcggcgt cactgtggta atgatggagc ggctgcaggc
    19501 aatcgccgga tcgaacctgc tgggcgatgg cgtgcactac aacgcgcaag ggcatgcgca
    19561 atttgccgcc tatgtgctgc cggaggtgag ggccgccgcc tgccgataga gcggcggcct
    19621 gctccaggtc ctacgatttg agcgcctttg cgatctcgtc cagggcggcg ggatcgtcga
    19681 tcgtcgccgg catggtccat gggtcgtggt cggcgatctt cttcatcgtg ccgcgcagga
    19741 tcttgccgga gcgtgtcttg ggcagccgcg cgacgacaac cgtatggcga taggcggcga
    19801 ccggtccgat cttctcgcgg atcagcgcgg cgcattcctt ctctatctcg accgccgatt
    19861 tggtaacacc cgccttcagc accacaaagc cgagcggggt ttcgcccttc agcgtgtcct
    19921 tggcgccgat caccgcacat tcggcgacgg acgggtggga ggcaagcacc tcctccatgc
    19981 cgccggtgga caggcgatgg ccggcgacgt tgatgatgtc gtcggtgcgg cccatgatgt
    20041 agagatagcc gtccgcatcg cgatagccgg cgtccgaggt cgagtaatag cccttgaact
    20101 cggagaggta gctttcgcga aagcggtcat cggcctgcca cagggtcggc agggcgccgg
    20161 gcggcatcgg cagcttgatg gcgatcgagc ccatcttgaa ggcttccacc tccttgccgg
    20221 attcatcgag gatatgtacc tcgaagccgg gcatcggcac ggtgggcgaa ccgggcttga
    20281 tcggcagcag ttcgacgccg gtggggttgc cggcgatggc ccagccggtc tcggtctgcc
    20341 accaatggtc gacgaccgga acgcccagca tcttctcggc ccagagcacg gtgtcggggt
    20401 cggcacgctc gccggcgagg aacagcgtct tgagcggctt gatgttgtac ttcctgatga
    20461 aggccccttc cgggtcctcc ttcttgatgg cacgcagggc ggtcggcgca gtgaacaggt
    20521 tgcggacctg atattcctcg atgatccgcc agaaggtgcc gggatcgggc gttccgacgg
    20581 gcttgccctc gaacaggatg gtggtgcagc caaccagcag cggcgcatag cagatgtagg
    20641 aatgaccgac cacccagccg acgtcggaag cggcccagaa cacctcgccc ggcagggcat
    20701 tgtagaggcc cttcatcgac caggcgagcg cgaccatgtg accgccggtg tcgcgcatca
    20761 cgcccttcgg cttgccggtg gtgccggagg tgtagagcac gtagagcgga tcggtcgcct
    20821 gcatcttctc cggcgcgcag cccttggcct tgccggaggc gcgcatggca tcgcgtgccg
    20881 ccgcccagtc ctcgtcgcgg ccggcgacca gtgcagcggt ctcctgcgga cgctggaaaa
    20941 tcaggcaggt ctcgggcttg gaggatgaca ggctgatcgc ctcatccagc agcggtttgt
    21001 atttgacgat gcggcccggt tcgatgccgc aggaggcgga gaggatggcc ttgggcttgc
    21061 aatcgtcgat tcgggtggcc agctcacgcg cggcaaagcc accgaacacc accgaatgga
    21121 ccgcgccgat gcgcgcgcag gcaagcatgc cgatcaccgc ctcgggaatc atcggcatat
    21181 acagcagcac gcggtcaccc ttgcggacgc cgcgatcacg cagcatctgg ccgagaacct
    21241 gaacctcgtc gagcagcgcg gcatagctga tgcgcgcctt ggtgccggta acgggagaat
    21301 cgtagaggat ggcatcctgc gcgccgcgtc cggcggcgac atgccggtcc aggcagttgt
    21361 aggcggtgtt gcacaccccg tccgggaacc agcgaccgta aacgccggcc ttctcgtcca
    21421 gaacggtctt ggggggcaca taccaatcga tcgccttggc ggcctcgccc cagaagcttt
    21481 tcgggtcctt ctgccagccc tgatagagtt tggaataggc gcccttgctg ctgccggcca
    21541 tgatttcctc ctgatttctg ttcggcgcgg attctcgacg tccagccggt ttacgcaaga
    21601 ggatcagcct catcacttcg atgaacggcc ggaacaaggc tgatttgcct gtcgatacct
    21661 tggcaatccc gccccggcgc gattgacgcg cgcttcaacc agcgcataac cttcctttca
    21721 caatcccttg aaggcaaacg atcagcgacg ctgcccatgt cccaggagcc ggttcaggag
    21781 gccctttccc gtttcagcca ggcactcgac cggctggagg ctgcctcgtt gcgccatcag
    21841 gagggcgagc ggctgcgcat cgcgctcgaa agcgaactgg cgctgatgcg ggccgaccgg
    21901 cagtcgctgg ccgaggcact cgaccagtcg gtggcgcgcg gacgccagct tgaggaacag
    21961 ttccagttga tcgaccagcg aatcgaacgg gccatcgcct cggtccgtga ttgcctcaac
    22021 gcggacggct gagccatgtc gcaggttcag atcacgattg acggcaagac cttccggatg
    22081 gcctgcgagg acgggcagga ggcgcatctt gaggcactcg gccgccatct cgacgggcgc
    22141 atcggcgaaa tgcgcaagac cttcggcgaa atcggcgacc tgcggctggt ggtcatggcg
    22201 gcgatcacca tcaccgacga attcttcgag gcgcggcggc ggaccgaaac actcggcaag
    22261 gaaatgcagc tggtgcgctc cgagcaatcc agcgttgccg ccgatgccgg ccagcgcgag
    22321 gcggagattg ccggggccat cgaccggatg acccagcggc tcgaacggat caccgcgctg
    22381 ctggcgacac gacccgatct gtaaggccgt agcaatagct gcccggatcg cctataatat
    22441 gcgggcggtg ctgcggggcg cgtcaggaaa cactatcccc ggggccttag cgatccttag
    22501 ggagctgtcc ctgaccgggt ccgtggaccc ggacacacgg cgcccaccta cttacgtagg
    22561 ttcccgggat cgacattcca acggctttcg cggctccgca cttcaggttt gtcttgcatg
    22621 acctctcccg acc
//
LOCUS       DAOMXG010000021        21531 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_1469_length_21531_cov_9.509080, whole genome shotgun sequence.
ACCESSION   DAOMXG010000021 DAOMXG010000000
VERSION     DAOMXG010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 21531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21531
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1469_length_21531_cov_9.509080"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1240
                     /locus_tag="PLQ11_02710"
     CDS             <1..1240
                     /locus_tag="PLQ11_02710"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRK23843.1"
                     /translation="EDLSVLENIALGTAGARADAALASRVAEVTKRYGLDLELGRPVW
                     TLSAGERQRIEIARCLLQEPRLIVLDEPTSVLTPQESEALFASLDQLAARGSGILFIS
                     HKLEEVRRLCSAATILRGGKLVANADPRRETARTLARLMVGEGVTDVRAVPHERPMTP
                     CLVIEDLSVQPDISHGIPLRGVDLEVRAGEVLAIAGVAGNGQSEFFAALSGEGGTVTA
                     GQIRLGETDVTRATIGRRRRLGAAFVPEGRLGHATLPQRNLSENIALSWHETEDVGGF
                     FLSLEKLRAVSRRVIQRFDVRGPSADPRAAQLSGGNLQKYIVGREIERNPKLLVVDQP
                     TWGVDARAAALIRQTLVDLARSGAAVVVITQDLDEAFEISDRIAVLCGGTMHTPVDTA
                     MTNRDAIGLLMTSTHSDVAA"
     gene            1237..2289
                     /locus_tag="PLQ11_02715"
     CDS             1237..2289
                     /locus_tag="PLQ11_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRK23844.1"
                     /translation="MIALVPRTAISRRREILTPLIAFAVAVALAALILLAMNRSLLVA
                     VNVYLIEPLASAWGIQEVLLKATPLTLIAVGLIFAVRANRWNIGAEGQFVVGGTLGGW
                     LAIATHGQEGVWILPAMLIAGMLGGLVYGLIPAILRNRFGISEILSSLMLVYVAELML
                     DFLVRGPWRDPRAFNFPKSVNFDAAATLGPIIEGGKLHWGIVLAGLAVVAATLLLGRS
                     LFGFAVSATGEAPRAANFAGFNADRLTLAVFALSGALAGLAGIIEVSANIGQLQPSIS
                     QGYGFTAIIVAFLGRLNPVGALVAALALAVLLIGSETAQIMLKLPFDMTRAFMGLLLL
                     CVLTGEALTRYRLVWK"
     gene            2297..3172
                     /locus_tag="PLQ11_02720"
     CDS             2297..3172
                     /locus_tag="PLQ11_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRK23845.1"
                     /translation="MDLALLILTTIATAATPLVIAGLGEHVVERAGVLNLGVEGMMIM
                     GAAVGFAGAVVLDSSFAGLLIGSLAGGAMAAVFGLFALGLATNQVATGLAVTIFGLGL
                     VGLIGQPFVGQPRPPIGSVLFGLDAVVLASLWLAVATAYVLKRTRTGLILRGVGESHG
                     SAHALGLPVLGMRWRAVLFGGLCAGLAGAYLSLVYTRFWSPGMTAGRGWIALALVVFA
                     AGSVRRLVAGAYLFGAILVAQLHAQAGAIGIPSQFLSMLPYLMTIVALVVLSLAGRRG
                     AGAAGSLGQPFVPDR"
     gene            3285..4349
                     /locus_tag="PLQ11_02725"
     CDS             3285..4349
                     /locus_tag="PLQ11_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HRK23846.1"
                     /translation="MLKKFMGALAVTAVLAAPAMAQQKLKVGFIYVGPVGDFGWSYQH
                     DQGRIAIEKALGDKVETSYIESVPEPDSERAIEQLARTGHKLIFTTSFGFMEPTLKVA
                     KKYPDVKFEHATGFKRDKNVSTYSAKFHEGRYIVGQIAGKMTKSNTIGYVGAFPIPEV
                     VAGINAFYLGAKSVNPNVKIKIVWANSWFDPGKEGDAAKALLDQGVDVITQHTDSPAP
                     LQQAAARGKFAFGQASDMTKFAEKNILSSIIDDWSPYYVERTKAVLDGSWTSKDTFDG
                     LAKGKVVMGPYVNMPDDVKAMAVATEKAIREGRMDPFKCPVVNQEGKTIECKGGDKLS
                     DEQVTSMNFYVQGIDDKLPK"
     gene            4554..5501
                     /locus_tag="PLQ11_02730"
     CDS             4554..5501
                     /locus_tag="PLQ11_02730"
                     /inference="COORDINATES: protein motif:HMM:NF015863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HRK23847.1"
                     /translation="MLKAWFLKLHGWIALIFSVPLAILCVTGLVLGFEPIVQYSTIEP
                     GTLNAEEIIRYLDRHDPQGKTTSIVHRPYENTLTLGGVGPEGELEIDLVTRDVTTSDG
                     GAWDWSEIFRYNRRLHEHVSVAGLELTVAATIAMCVIILLGVLLGLPVLRNSVAGWHK
                     AIAWFGLPFLIISPLTGLFIAWGVSMNIAPAPTPERSAPLPIREAVQIAGTSHDLSNL
                     VWLRQRGGRQMIRLWDGQEARSYAIARSGLVPMPRNVSRMIHEGNFLGIWSGILKVLT
                     GVAFVVLMVTGLWLFIRKQVRKARNRQARRNEAAGSAAS"
     gene            complement(5507..6823)
                     /gene="rimO"
                     /locus_tag="PLQ11_02735"
     CDS             complement(5507..6823)
                     /gene="rimO"
                     /locus_tag="PLQ11_02735"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018427901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HRK23848.1"
                     /translation="MSSAPRISFVSLGCPKALVDSERIITRLRAEGYDLARTHAGSDV
                     VIVNTCGFLDSAKAESLDAIGTAMKENGKVIVTGCMGAEPEQIRDRFPNVLAITGPQA
                     YESVMAAVHQAVPPAHDPFVDLVPEQGVKLTPRHYAYLKISEGCNNRCTFCIIPKLRG
                     DLVSRPAADVLREAEKLVKAGVKELLVISQDTSAYGVDVKYAPSQWGDREIRTRFLDL
                     SRELGQLGVWVRLHYVYPYPHVDAVIDLMAEGLVLPYLDIPFQHAAPNVLKAMKRPAH
                     QEKTLERIRAWRTTCPDLAIRSTFIVGFPGETEEDFTELLDFLTEAKLERVGAFKYEP
                     VKGALANDLGLPMIAPEVQQQRYDRFMKHQQAISARLQQRRIGQRVPVIIDSAGPGGA
                     RGRTKWDAPEIDGAVHVASRRPLRPGDIVSVKIERAEAYDLYGLAV"
     gene            complement(6931..7734)
                     /locus_tag="PLQ11_02740"
     CDS             complement(6931..7734)
                     /locus_tag="PLQ11_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain 1 protein"
                     /protein_id="HRK23849.1"
                     /translation="MAISAAELAVLVKITGAFENSGDPYQGVSGDFDGQGISCGVLQW
                     NIGQGSLQRLLKDLGQARVKALMPTYGTQMWQAASVSIPAGLAIVRGWQTNKVLKATP
                     RAELKAMMGASDMKAIQDKYIAQVGIKAEGYANAWAAASGMPSRTNQQLAFFFDIVTQ
                     NGSTSGLTFQDVKDFKAATTPGNADDLVCDWMLSRPKNMAGKKDGDKNAALWRNKLSG
                     DAYNLLVFAYLRSQKANPKWRLDVMNRKGTLAARKGWVHQGLRDLTGII"
     gene            complement(7745..8326)
                     /locus_tag="PLQ11_02745"
     CDS             complement(7745..8326)
                     /locus_tag="PLQ11_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23850.1"
                     /translation="MTNMKNEGFEIRVIRKSIQKRTVGVVARTRTVGTYAVYIDGVQQ
                     PGLRGTMAEQKGPSDNSSDGAKYDRRIAPGRYPLWTQNGTKYKTFGYLDTPAAQKQFK
                     YKPRPGIELKETGKRAEILIHPASGFLSSEGCMHPSNALADGNADIDHAAESRPRVLA
                     LIKAMKDYCGNSFPKTNGKRLPNCWCVVEDDFS"
     gene            8430..8918
                     /locus_tag="PLQ11_02750"
     CDS             8430..8918
                     /locus_tag="PLQ11_02750"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRK23851.1"
                     /translation="MALMDGATFGVALQQVGPDRTGLDILRAMISGELPAPTICEGLS
                     FRLIEAEEGRAVFAGETSGAILNPHGTVHGGWALTLIDSATGCAALSTLPAGTGYTTV
                     ETKGNFVRPILAETGLVRCEGIVLARGRQIITAEARITDSAGKLLAHGSATMLVLPAR
                     GG"
     gene            complement(8937..9308)
                     /locus_tag="PLQ11_02755"
     CDS             complement(8937..9308)
                     /locus_tag="PLQ11_02755"
                     /inference="COORDINATES: protein motif:HMM:NF025607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4345 domain-containing protein"
                     /protein_id="HRK23852.1"
                     /translation="MKSPVLIVAGLMMVVIGTLHLIAPQMMMETPGVALTSTNHFHII
                     RAAYGGAYIGIGVLFLLGAWCADFRRSSLLAVAVLFGGFAAGRLVSILVDGVPVALYL
                     VVLAFELVCALFAIRALLRSS"
     gene            9581..9670
                     /locus_tag="PLQ11_02760"
     tRNA            9581..9670
                     /locus_tag="PLQ11_02760"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9615..9617,aa:Ser,seq:tga)
     gene            complement(10050..11507)
                     /locus_tag="PLQ11_02765"
     CDS             complement(10050..11507)
                     /locus_tag="PLQ11_02765"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRK23853.1"
                     /translation="MNSRLMVGILGLGLSTAISGLAGAADLPNRKVAPVAFAPTTAAS
                     WTGIYLGVQAGVGMSAAEIKLPGGTGGLDGLMGRGLFGGVRLGVDYQVSPNVVLGLIG
                     TADFGDIGGKAKLGSETFSQEALRIDGNKFVGPADQTVGPYPGTLTYTADGGVRVRTT
                     DVLGLRARLGYLVTPDTLVYIAPGWASAKGTLKGNLGTITMVDSTGGSMTDVYNASFR
                     QSKRINGWQLGVGFESRLTDNLSANFEYVYSQFQDLRIGGSDVKASAATGAFTAGLNY
                     RFGVNAGAAPVRVAAAAPENWTGLYAGLSGGAGIGGAKLKLGNMASVDGFGSVGLAGG
                     VVLGYDYQIAPRWVIGAEFGGDLSSYDVSARETGANAGAKISMPWAITARARAGYLIS
                     PGTMLFASLGVTGGQFRVKADNAGFGIRESSSSYGAMVGIGGGFQTAMSKNVFLRMEY
                     QHSMLPAIKVKSIGSVPVSFEPTAGAARISLIYKM"
     gene            complement(11727..12488)
                     /gene="tpiA"
                     /locus_tag="PLQ11_02770"
     CDS             complement(11727..12488)
                     /gene="tpiA"
                     /locus_tag="PLQ11_02770"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRK23854.1"
                     /translation="MQKRPRPLVAGNWKMNGTRAALAEIKAIAEGYDVALQNRAALLI
                     CTPATLVLTAAAASLGSRLEIGGQDCHMKASGAHTGDVSAEMLADAGASFVIVGHSER
                     RADHAETNAIVKAKAEAALRAGLRAIVCVGETRAEREAGKTLAVVRKQVKNSLPDGAN
                     RWNTIIAYEPVWAIGTGLTPTEADVAEVHADIRKTLKGALGADTAGRMRILYGGSVKP
                     DNAASLMAVANVDGALIGGASLKAKDFLAIAQAYK"
     gene            12621..13505
                     /locus_tag="PLQ11_02775"
     CDS             12621..13505
                     /locus_tag="PLQ11_02775"
                     /inference="COORDINATES: protein motif:HMM:NF024859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRK23855.1"
                     /translation="MADHHTSTPRGIDHAVIATRDLDALANSFAELGFKVGARNRHPW
                     GTENHIVQFDGSFIELIGIGAGASIAGHEPGRFSFGGFVRDYLEDGEGMAMLALESHN
                     ATGDREAFRKARIGDFSRFDFARSGQGAEGKPVDVAFSLAFAELSGSPRAAFFVCQQH
                     KPQNFWSRAVQKHPNGATGIASVVVVADDPSDTHEFLGAFVNQREMRATSFGLDIDTG
                     RGRIEVLSPDAFAYRFGAPAPARPKGGAQFAGLTIAGASVARLEKVTQAARKVVTARQ
                     TMLVTELFGGACQLGFDV"
     gene            13515..13865
                     /locus_tag="PLQ11_02780"
     CDS             13515..13865
                     /locus_tag="PLQ11_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23856.1"
                     /translation="MKAIVFVVIGGVAALLGWLFFQPECFQGVVVASEHACVQVPGFD
                     RQFCERAFARPEEAIFRAGSFYQTRDDCLRRHTDCIEFPGVHGYTPKPTGYCVLRGAD
                     GSLAGMKPVYGRPS"
     gene            13985..14833
                     /gene="kdsA"
                     /locus_tag="PLQ11_02785"
     CDS             13985..14833
                     /gene="kdsA"
                     /locus_tag="PLQ11_02785"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HRK23857.1"
                     /translation="MVMTSKSDANEVRIGSLTLSNDLPLTLIAGPCQMESRDHAFAAA
                     GFLKDMADRLGIGLIYKSSFDKANRTSLNAARGLGLEKALGVFADLKAAFGFPVVTDV
                     HEAGQCAEAAGVVDVLQIPAFLCRQTDLLVAAAKTGRVVNVKKGQFLAPWDMANVVAK
                     LRGAGNPNVLVTERGVSFGYNALVSDMRALPIMAETCRAPVIFDATHSVQQPGGQGGS
                     SGGERRFVAALARAAVAVGVAGVFIECHEDPDHAPCDGPNMVALKDMPALIERLMAFD
                     RLAKGL"
     gene            14921..16282
                     /gene="glmU"
                     /locus_tag="PLQ11_02790"
     CDS             14921..16282
                     /gene="glmU"
                     /locus_tag="PLQ11_02790"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HRK23858.1"
                     /translation="MAATTIAVILAAGEGTRMKSGLPKVLHKVAGRSLLGHALLGVKD
                     AGLDGAAVVIGPNHGKVEDEVRSVLSGADVFVQAERRGTAHAVLQARAAIARGHAAIV
                     VLFADTPLVRSETVRRLADAVLKGGDIGVMCFRAKDPTGYGRILMEGGEIIAIREQKD
                     ATEAERRITLCSGGLMSFKGEHALRILDAIDCNNAQNEYYLTAAVEVARRMGLRAVPV
                     EVAEEEAFGVNDRAQLASAEAVVQTRLRRAALLAGVTLQAPGTVYFSADTRLGCDVVI
                     EPNVYFGPGVTVGDGVVIHAFSHLEGAHVGKGASVGPFARLRPGATLAAEVKVGNFVE
                     IKNASVEAGAKISHLSYIGDARIGAEANIGAGTITCNYDGYRKFVTDIGAGAFIGSNS
                     SLVAPVTIGDGAFVGSGSVVTKDVPADALAVARGHQMVKAGWARSFRARPENVAKARK
                     KKD"
     gene            16365..17927
                     /gene="guaA"
                     /locus_tag="PLQ11_02795"
     CDS             16365..17927
                     /gene="guaA"
                     /locus_tag="PLQ11_02795"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020812399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HRK23859.1"
                     /translation="MTALPHHETVLIIDFGSQVTQLIARRVRESGVYSEVHPFQSAEA
                     AFRALKPKAVIFSGGPDSVTRPGSPRAPQAVFDSGVPILAICYGQQTLCTQLGGTVEG
                     GHAAEFGRADVEVKAPCALFEGIWEVGGRYPVWMSHGDRVTRLPAGFTVKAQSENAPF
                     AVASDEKRRYYTTMFHPEVVHTPDGAKLISNFVHRIAGLAGDWTMKAFRAEQIDRIRA
                     QVGKGRVICGLSGGVDSAVAAVLIHEAIGDQLTCVFVDHGLMRQGEAGEVVRLFRDHY
                     NIPLVHADASETFLGALAGVSDPEVKRKTIGKLFIDVFEAEAKKVGGAGFLAQGTLYP
                     DVIESVSFTGGPSVTIKSHHNVGGLPERMNMKLVEPLRMLFKDEVRALGLELGLPEIF
                     VGRHPFPGPGLAIRCPGEITPEKLDILRKADAIYLEEIRNAGLYDRIWQAFAVILPVR
                     TVGVMGDGRTYDHVLALRAVTSVDGMTADFFPYDMAFLGRTATRIINEVRGINRVVYD
                     VTSKPPGTIEWE"
     gene            complement(17975..19759)
                     /locus_tag="PLQ11_02800"
     CDS             complement(17975..19759)
                     /locus_tag="PLQ11_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23860.1"
                     /translation="MFEIIRRTFPEGTDDLAGTDVSKAPDWPPDLFAVCAFLLERSGT
                     YQNINPGHGDPAGSVNPTLTHADHEALLGLADEWKKRLSTVPAGVQALWTELLDDGKT
                     KKFNVRIKTGETLPAWVIAAYKLLIIADEVCENTGYVDAPPSSGTPTTVAVIVAFNET
                     NARARGRKHFFHAHGTHSTPVPIFSITQRASMDMLAVQPKSRTAQVGVTMRTFSKHLA
                     LLPPVTRINTAWQRFATAGSDDGEDLNLLLIPFPYRIEAGWWTAKTVETNPPLKWGNF
                     ELEQRWLEGMTAEQLGAFVSLLAEKSGRTIHGVVFPELSLTTALHRDLTEQLASTNPE
                     IEFVIAGSSDNCDDDKGNFALVSNIYRAPAGGSSGSAAPPANIEHISQGKHHRWRIDP
                     RQCIAYGLTGKLCDDTDWWETMELGRRQIYTHAFRNKSSFAVLICEDLARTDPCHETI
                     RSLGPNIVIALLMDGVQIAQRWPGYYSLGLSEDPGSSVLTFTSRGLIQRAQDENRKQA
                     TVMGKSARPPNWSVAMWRDGSSAEVRELHCPPGNDAIILCIKGRKIPERTFDGRIRPE
                     AIRWEFRSAEAFGLSDADRSRMPKAPVG"
     gene            20833..>21531
                     /locus_tag="PLQ11_02805"
     CDS             20833..>21531
                     /locus_tag="PLQ11_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HRK23861.1"
                     /translation="MTERNGAKLAADRNGRAARSLDGAAHRLARFCVAGMVGLLVANC
                     ASQPQRAARSTSKEVGAFADSSRYGNASPRVVAEGQDVPKGGGKYHIGNPYRIAGRTY
                     YPSDRPVGHTQSGMASWYGEAFHGRKTANGEVYDRRSYSAAHPTMPLPSYARVTNLRN
                     NHSVIVRVNDRGPYHGGRVMDVSERVANALDFKHTGTTRVRIDYLGKAGLAGSDDVRL
                     AASLRTDGTPASSPG"
BASE COUNT         3701 a         6613 c         7039 g         4178 t
ORIGIN      
        1 tgaggatctc agcgtgctgg aaaacatcgc gctgggcacg gccggggcgc gggcggatgc
       61 cgcgctggcc agccgcgtcg ccgaggtgac aaaacgctat ggcctcgacc tcgaactggg
      121 ccggccggtg tggacgctct cggccgggga gcgccagcgt atcgagatcg cccgctgcct
      181 gttgcaggag ccgaggttga tcgtgctcga tgagccgacc tcggtgctga ccccgcagga
      241 gagcgaggcc ttgttcgcct cgctcgatca actggccgcg cgcggatccg gcatcctgtt
      301 catttcgcac aagctggagg aagtgcggcg gctgtgttcg gcggccacca tcctgcgcgg
      361 cggcaagctg gtggccaatg ccgatccgcg ccgggagact gccaggacgc tggcacgcct
      421 gatggtcggc gagggcgtga ccgatgtgcg cgccgtgccg catgaacggc cgatgacgcc
      481 ctgcctggtc atcgaggacc tgtcggttca gccggacatt tcgcatggca ttccgctgcg
      541 cggcgtcgat ctggaggtgc gggccggcga ggtgctggcc atcgccggcg tcgccggcaa
      601 cgggcagagc gagttttttg ccgcgctgtc gggcgaaggg ggaaccgtca ccgccggaca
      661 gatccggctg ggcgagacgg atgtcacccg cgcgaccatc ggccgccgcc gccggctggg
      721 ggctgccttt gtgccggaag gccggcttgg ccatgcgacc ctgccgcagc gcaacctgag
      781 cgagaatatc gccctgagct ggcacgaaac ggaagatgtc ggtggcttct tcctgtcgct
      841 cgaaaagctg cgcgctgttt cccgcagggt gatccagcgg ttcgatgtgc gcggcccctc
      901 ggccgatccg cgcgccgcgc agctttccgg cggcaatctg cagaaataca tcgtcgggcg
      961 cgagatcgag cgcaatccca agctgctggt tgtcgaccag ccgacctggg gcgtcgatgc
     1021 ccgggcggcg gccctgatcc ggcagacgct tgtcgatctg gcccggtccg gggcggccgt
     1081 ggtggtgatc acgcaggatc tcgacgaggc tttcgagatt tccgaccgga tcgccgtcct
     1141 ttgcggcggc accatgcata ccccggtcga caccgcgatg accaatcgcg acgccatcgg
     1201 actgctgatg acctcgaccc attccgacgt ggccgcatga tcgcgctggt tccccgcacc
     1261 gcaatcagcc gccgccgcga gatcctgact ccgctcatcg cctttgcggt ggcggtggct
     1321 ttggccgcgc tgatccttct agcgatgaat cgatcgctgc tggtggccgt caatgtctat
     1381 ctgatcgagc cgctcgcctc tgcatggggt atccaggagg tcttgctcaa ggcgacgccg
     1441 ctgacgctga ttgccgtcgg cctcatcttc gcggtccggg ccaaccgctg gaacatcggc
     1501 gccgaggggc agttcgtggt tggcggcacg ctgggaggct ggcttgccat cgccacgcat
     1561 gggcaggagg gcgtctggat cctgccggcg atgctgatcg ccggaatgct cggcggcctc
     1621 gtttacgggc tgattcccgc catcctgcgc aatcgcttcg gcatttcgga gatcctgtcg
     1681 tcgctgatgc tggtctatgt cgccgaactg atgctcgatt tcctggtgcg tggaccgtgg
     1741 cgcgacccgc gcgccttcaa tttccccaag agcgtcaatt tcgatgctgc ggcaacgctg
     1801 gggccgatca tcgagggcgg caagctgcat tggggcatcg tgctggccgg gctggctgtc
     1861 gtggcggcga cgctgctgct ggggcgcagc ctgttcggct ttgcggtgag cgcgaccggc
     1921 gaggcgccgc gcgctgccaa tttcgccggg ttcaacgccg accgtctgac cctggcggta
     1981 ttcgcgctgt cgggggctct ggcggggctt gccgggatca tcgaggtctc ggccaacatc
     2041 ggccagctgc aaccctcgat cagccagggc tacggcttta ccgcgatcat cgtcgccttc
     2101 ctcgggcggc tgaacccggt cggcgcgctg gtggcggcac tggcgctggc tgtgctgctg
     2161 atcggttcgg agacggcgca gatcatgctc aagctaccgt tcgacatgac gcgcgccttc
     2221 atggggctgc tgctgttgtg cgtgctgacc ggcgaggcgc tgacgcgcta tcgcctggtg
     2281 tggaagtgag gcgataatgg accttgcgct gctgatcctg acaaccattg ccaccgccgc
     2341 gacgccgctg gtgatcgccg gtctcggcga gcatgtggtg gagcgcgccg gtgtgctcaa
     2401 cctcggtgtc gagggcatga tgatcatggg cgcggcggtc gggtttgccg gcgccgtggt
     2461 gctcgactcc agctttgccg ggctgttgat cggctcgctg gccggcggcg caatggcggc
     2521 ggtctttggc ctgttcgcgc tcgggctggc gaccaatcag gtggcgaccg gcctggcggt
     2581 gacgattttc ggcctggggc ttgtcgggct gatcggccag ccgttcgtcg gccagccgcg
     2641 cccgccgatc ggctctgtgc tgttcgggct cgatgcggtg gtgctggcct cgctgtggct
     2701 tgcggtggcg actgcctatg tgctcaagcg cacccgcacc gggttgatcc tgcgcggcgt
     2761 cggggagagc catggttctg cccatgcgct tggcctgccg gttctgggga tgcgctggcg
     2821 ggcggtgctg ttcggcggcc tgtgcgccgg gttggcgggg gcgtatctgt cgctggtcta
     2881 tacgcggttc tggtcaccgg gcatgacggc ggggcgcggc tggatcgcgc tggcgctggt
     2941 ggtttttgcg gctggatcgg tgcggcggct ggtggcaggt gcctatctgt tcggcgcgat
     3001 cctggtggcg caactgcatg cgcaggccgg cgccatcggc ataccctcgc agttcctgtc
     3061 gatgctgccg tatctgatga cgattgtggc gctggtggtg ctgtcgctgg ccgggcggcg
     3121 aggggcaggg gcggcgggct cgcttggcca gccgttcgtt ccggaccgtt agccgtcgtg
     3181 cgccgaacca gtagcaaaaa cgtgcgttga aacagaaaaa cgccccttgc gcggtccggc
     3241 gccgcttgaa ataacggaac aaagcaaact tcgaggaggc aagaatgctg aaaaaattca
     3301 tgggcgcgct ggcggtgacc gccgtgctcg ccgcccctgc catggcccag cagaagctga
     3361 aggtcggctt catctatgtc ggcccggtcg gcgatttcgg ctggtcctac cagcacgatc
     3421 agggccgcat cgccatcgag aaggcgctcg gcgacaaggt cgagacctcg tatatcgaaa
     3481 gcgtgccgga gccggattcc gagcgcgcca tcgagcagct tgcccgcacc ggccacaagc
     3541 tgatcttcac cacctcgttc ggcttcatgg agccgacgct gaaggtcgcc aagaagtatc
     3601 cggacgtgaa gttcgagcac gccaccggct tcaagcgcga caagaacgtc tcgacctatt
     3661 cggccaagtt ccacgagggc cgctatatcg ttgggcagat cgccggaaag atgaccaagt
     3721 cgaacaccat cggctatgtc ggcgccttcc cgatcccgga agttgtggcc ggtatcaacg
     3781 ccttctatct cggcgccaaa tcggtcaatc cgaacgtcaa gatcaagatc gtctgggcca
     3841 attcctggtt cgatccgggc aaggagggcg atgccgccaa ggcgctgctc gatcagggcg
     3901 tcgatgtcat cacccagcac accgacagcc cggcaccgtt gcagcaggcg gcggcgcgcg
     3961 gcaagttcgc cttcggtcag gcatccgaca tgaccaagtt cgccgagaag aacatcctgt
     4021 cctcgatcat cgacgactgg tcaccctact acgtcgaacg caccaaggcc gtgctggatg
     4081 gcagctggac ctcgaaggac acgttcgatg gacttgccaa gggcaaggtc gtgatgggcc
     4141 cgtatgtcaa catgccggat gatgtcaaag ccatggcggt cgccaccgaa aaggcgatcc
     4201 gcgaaggcag gatggacccg ttcaagtgcc cggtcgtcaa tcaggaaggc aagacgatcg
     4261 agtgcaaggg cggtgacaag ctgtccgacg agcaggtgac ctcgatgaac ttctacgttc
     4321 aggggatcga cgacaagctg cccaaatagg ccaccctttc gccgaaaatc cctcatcccg
     4381 ccgttgctgg cggggtgagg gcgcctgatc ccgccggggg gggtagcctt gcctggcaac
     4441 ctgcttcacc gtgacccccg cgttactttt tgcattgcaa aaatttcact tgagatttgg
     4501 ccgtcacagc ctatccgttg acggatgttg atagttgctg gcggagcacg atcatgctga
     4561 aggcctggtt tctcaaactg cacggctgga ttgcgctgat cttctcggtg ccgctggcca
     4621 ttctgtgcgt aaccgggctg gtcctcgggt ttgaaccgat cgttcagtat tccaccatcg
     4681 agccgggcac gctgaacgcc gaggaaatca ttcgctatct cgaccggcac gacccgcagg
     4741 gcaagaccac ttccatcgtg catcggccct acgagaacac gctgaccctt ggcggggtcg
     4801 gcccggaagg cgagcttgag atcgatctgg tgacgcgcga tgtcacgacc agcgacggcg
     4861 gtgcctggga ttggtcggag attttccgct ataatcgccg gctgcacgag catgtcagcg
     4921 tcgccggcct cgaactgact gtcgccgcga cgatcgcgat gtgcgtcatc atcctgctgg
     4981 gcgtgttgct gggcctgccg gtccttcgca attcagtggc cggctggcac aaggcgattg
     5041 cctggttcgg cctgccattt ctcatcatta gcccgctgac gggcctgttc atcgcctggg
     5101 gtgtgtcgat gaacatcgcc ccggcgccga cgccggagcg gtcagccccg ctgccaatcc
     5161 gggaggctgt gcagattgcc gggacttcgc atgatctgag caacctcgtc tggctgcgtc
     5221 agcgcggtgg ccggcagatg atccgccttt gggacgggca ggaggcgcgc tcctatgcga
     5281 ttgccaggag cggcctcgtg ccgatgccgc gcaatgtctc gcgcatgatc cacgagggca
     5341 atttccttgg catctggtcc ggcattctca aggttctgac cggtgtcgcc tttgtcgtgc
     5401 tgatggtgac cggcctgtgg ctgttcatcc gcaagcaggt gcgcaaggcc cgcaaccgtc
     5461 aagcgcggcg gaacgaggct gccggttccg ctgcgagctg aaccggtcag accgccagcc
     5521 cgtagagatc ataggcttcg gcgcgctcga tcttgaccga cacgatatca cccggtcgca
     5581 gcggccgccg cgaggcgaca tgcactgcgc cgtcgatctc cggcgcgtcc catttcgtcc
     5641 gtcccctggc cccgcctggc ccggcggaat cgatgatcac cggcacgcgc tggccgatcc
     5701 ggcgctgctg gaggcgtgcc gagatggcct gctggtgttt catgaaccgg tcatagcgct
     5761 gctgctgcac ttcgggtgcg atcatcggca ggccgagatc attggccaag gcccccttga
     5821 ccggctcgta tttgaaggcg ccgacacgct ccagcttcgc ctcggtgagg aaatcgagca
     5881 attcggtgaa atcctcctcc gtctcgccgg ggaagccgac gatgaaggtg gagcggatcg
     5941 ccagatcggg gcatgtggtg cgccaggcgc ggatgcgctc aagcgtcttt tcctgatgcg
     6001 cgggccgctt catcgctttg agcacattcg gcgcggcatg ttggaacgga atgtcgagat
     6061 agggcagcac cagcccctcg gccatcagat cgatcactgc atcgacatgc ggatagggat
     6121 agacatagtg caggcgcacc cagacgccga gttggccgag ttcacgcgaa aggtcgagaa
     6181 accgggtgcg gatttcgcga tcaccccatt gcgagggtgc atatttcaca tcgacgccat
     6241 aggcggaggt atcctgcgag atgaccagca gttccttgac gccggccttg accagctttt
     6301 ccgcctcgcg cagcacatcg gccgcagggc gcgaaaccag atcgccgcgc agcttcggga
     6361 tgatgcagaa ggtgcagcga ttgttgcagc cttcggaaat cttcagatag gcgtagtggc
     6421 gcggggtgag tttcaccccc tgttccggca ccagatcgac aaacggatca tgggccggcg
     6481 gcactgcctg atgcaccgct gccatcacgc tttcgtaagc ttgcgggccg gtgatggcga
     6541 gcacgttggg aaagcggtcg cggatctgct cgggctcggc ccccatgcat ccggtgacga
     6601 tgaccttgcc gttctccttc atcgccgtgc cgatggcatc gagcgattcc gccttggcgc
     6661 tgtcgagaaa gccgcaggtg ttgacgatca ccacatccga accggcatgc gtcctggcaa
     6721 gatcgtagcc ttcggcgcgc agccgcgtga tgatccgctc ggaatcgacc agcgccttcg
     6781 ggcagccgag cgagacgaag gaaatgcggg gcgccgacga catccggtga tcctgtggca
     6841 agaacggtgc gtcgtgtgcg gcaggtggac ggtcctgtca accggaatgg cgtccggatg
     6901 ccccggccgc cacccttggc tgtctttgtt tcagatgatg ccggtcagat cacgcaatcc
     6961 ctgatgcacc cagcccttgc gtgcagccag cgtgcccttg cggttcatca catcgaggcg
     7021 ccattttgga ttggccttct ggctgcgaag ataggcaaag accagaaggt tgtaggcatc
     7081 gcctgacagc ttgttgcgcc agagggcggc attcttgtcg ccgtccttct tgccggccat
     7141 gttcttggga cgggacagca tccagtcaca gacgaggtca tcggcattgc ccggtgtcgt
     7201 tgctgccttg aagtccttga catcctggaa cgtcaggcca gacgtcgaac cgttctgggt
     7261 gacgatgtcg aaaaagaacg ccagttgctg gttggtccga ctgggcatgc cgctggctgc
     7321 ggcccaggcg ttggcatagc cttccgcctt tatcccgacc tgcgcgatat acttgtcctg
     7381 aatggccttc atgtcgctcg cgcccatcat ggccttgagt tcggcgcggg gcgtggcctt
     7441 cagaaccttg ttggtttgcc agccccggac gatcgccaga cccgcaggaa tggagaccga
     7501 tgccgcctgc cacatctggg ttccgtaggt cggcatcagt gccttgacgc gggcctggcc
     7561 gaggtctttc agcaaccgtt gcaggctgcc ctggccgatg ttccattgca atacgccgca
     7621 ggaaataccc tgtccgtcaa aatcgccgga aaccccctga tagggatcgc cggaattctc
     7681 gaaagcgccg gtgatcttga ccagaaccgc cagttcggct gctgaaatcg ccatgtcatc
     7741 ctcctcagga aaaatcgtcc tcgacaacac accagcaatt gggcaggcgc ttgccgttgg
     7801 ttttgggaaa actgttgccg cagtagtctt tcatcgcctt gatcagcgcg agcacgcggg
     7861 gccggctttc ggcggcatgg tcaatatcgg cattgccatc cgccagcgca ttgctcgggt
     7921 gcatgcaacc ttcggacgac aggaatccgg aggccgggtg aatgaggatt tccgcgcgct
     7981 tgccggtttc cttgagttcg atgcccggcc gcggcttgta cttgaactgc ttctgcgcag
     8041 cgggcgtatc gagatagccg aacgtcttgt atttcgttcc gttctgcgtc cagagcgggt
     8101 agcgtccggg ggcaatccgg cggtcgtatt tggcgccgtc ggaagaattg tcgctcggcc
     8161 ccttctgttc ggccatggtt ccgcgcagcc ccggttgctg gacgccgtcg atatagacag
     8221 catacgtgcc gactgtgcgt gtccttgcaa ccaccccgac tgtccgcttc tggatggact
     8281 tacggatcac gcggatttcg aatccttcgt ttttcatatt ggtcacgatc gtgtttcctc
     8341 ccgctcctga tgacggatgg cgcatgttga gcgggggcgc cccttgtgcc aacattcgat
     8401 cggatgacgg gaatggagaa actcacccca tggcattgat ggatggcgca acgttcggcg
     8461 tggcgctgca gcaggtcgga ccggaccgca ccgggctcga cattcttcgc gcgatgatat
     8521 ccggcgaact gccggcgccg accatctgcg aggggctttc gttccggctg atcgaggcgg
     8581 aggaggggcg ggcggtgttt gcgggcgaaa cctcgggcgc cattctcaat ccgcacggaa
     8641 ccgtgcatgg cggctgggcg ctgacattga tcgacagcgc gaccggttgc gccgcattgt
     8701 caacgcttcc ggctggcacc ggatatacga cggtcgagac caagggcaat ttcgtccgcc
     8761 cgatccttgc cgagacagga ctggtgcgct gtgaaggcat cgtgctggcg cgcggtcggc
     8821 agatcatcac ggcggaggca agaatcaccg attccgccgg caagctgctg gcccatggca
     8881 gcgcgacgat gctggttttg ccggcgcggg ggggctgaac tcgtctcaat cgcccgtcat
     8941 gaggaccgga gcaaggcccg gatggcgaac agggcgcaga ccagttcgaa ggccagaacg
     9001 accagataca gggccaccgg cacaccatcg acgaggatgg agaccaggcg tccggccgca
     9061 aagccgccga acagcacggc taccgccagc agcgacgacc tgcggaagtc ggcacaccag
     9121 gcgccgagca ggaacagcac gccaatgccg atataggccc cgccataggc cgcccggatg
     9181 atgtggaaat ggttggtcga ggtgagcgcc acgcccggcg tctccatcat catctgcggg
     9241 gcgatcaggt gcaatgtgcc gatcaccacc atcatgagac cggcaacgat caggacagga
     9301 gatttcatgg cggcggatcc tcgttcggca cgtgcgggcg gggccccggt ttgtcgttcg
     9361 gggccagcca ttgttccgcc aaatgatgtc tgtggcaacg gcatttcgct actgcatgat
     9421 gcagggcttg cggcgcgccc ggtttcttgc tctagatcac gctctcgttt cgatggaatc
     9481 atcaaaacga gagataacgt gatcgttatc aagtgtttag agcatgcgtt ggcgattaac
     9541 cggcttccac ttaatcgcag cctgctctag caggaatgtc ggaagggtgg ccgagtggtt
     9601 taaggcagcg gtcttgaaaa ccgccgtgga tgcaagtcca ccgtgggttc gaatcccacc
     9661 ccttccgcca gttatgggcg cgacctcgaa cccacggttc atttgcaatc gcgcggtcgg
     9721 ccttggccgg agcgcgattg aagaggtctg gggttcgaat cccacccctt ccgccagtta
     9781 tgagcgcgac ctcgaaccca cggttcattt gcaatcgcgc ggtcggcctt ggccggagcg
     9841 cgattgaaga gatttggggt tcgaatccca ccccttccgc cagttatgag cgcgacctcg
     9901 aaccggacag ggccagaatt ccaggcacaa aaacgggcgc cagatctgca tccggcgccc
     9961 ggctgttcgg gcttacaaaa tggcctggcc tttatcgcat tttcgtcacg cgaaccggat
    10021 tccccttcgc ttgaacatgc tccagcccat tacatcttgt agatcagcga gatgcgggct
    10081 gcgccggcgg tcggctcgaa gctgaccggc accgagccga tgctcttgac cttgatcgcc
    10141 ggcagcatgg agtgctgata ctccatccgc aggaacacgt tcttggacat tgccgtctgg
    10201 aaaccgccgc cgatgccgac catcgcacca taggaggacg agctttccct gatgccgaag
    10261 ccggcattgt cagccttgac ccggaactgg ccacccgtca cgccgagcga ggcgaacagc
    10321 atggttcccg gcgagatcag gtaaccggcg cgggcgcggg cggtaatggc ccacggcatc
    10381 gagatcttgg cgccggcatt ggcgccggtc tcgcgggcgc tgacatcata gctcgacaga
    10441 tcgccgccga attcggcgcc aatcacccag cgcggggcga tctggtagtc gtagccgagc
    10501 acgacgccgc cagccaggcc gaccgagccg aagccgtcaa ccgaggccat gttgcccagc
    10561 ttgagcttgg caccgccgat gcccgcgccg ccgctcaggc cggcataaag gccggtccag
    10621 ttttcgggcg ctgccgctgc gacccggacc ggcgcagcgc cggcgttgac gccgaagcga
    10681 tagttgaggc ctgcggtgaa cgcgccggtc gcggcgctgg ccttgacgtc cgatccaccg
    10741 atgcggagat cctggaactg gctgtagaca tattcgaaat tggccgagag attgtcggtc
    10801 aggcgcgatt caaagccgac gccaagctgc cagccgttga tccgcttgga ctggcggaac
    10861 gaggcgttgt agacatcggt catgctgccg ccggtggaat ccaccatggt gatcgtgccg
    10921 aggttcccct tgagcgtgcc ctttgccgaa gcccagccgg gcgcgatata gaccagcgta
    10981 tcgggcgtca ccagatagcc gagacgggcg cgcaacccga gcacgtcggt cgtcctgacc
    11041 ctcaccccgc catcggcagt gtaggtcagc gtgccggggt agggaccgac cgtctgatcc
    11101 gcagggccga cgaacttgtt gccgtcaatc cgcagcgctt cctgcgagaa agtctcggaa
    11161 ccgagcttgg ccttgccgcc gatatcgccg aaatcggcgg tgccgatcag acccaggacg
    11221 acattgggtg aaacctgata atcaacgccg agacggacgc cgccgaacag gccgcgaccc
    11281 atcagcccgt cgagcccgcc cgttccgcct ggcagcttga tctcggcggc cgacatgccg
    11341 acgccggcct gcacgccaag atagatgccg gtccagctcg ctgcggtagt cggcgcaaag
    11401 gccaccggcg caaccttgcg gtttggaaga tcggcggcgc ccgccaagcc ggaaatcgcg
    11461 gtgctgagcc caaggccgag aatgccaacc atcaaacgag aattcatcat tgctcaccca
    11521 aatcttgaat aactgcaatt gcccctatgc gttcttagca aggccggacc tgatgacaag
    11581 gggatggact gtcacacgga aaaccaggaa acccctgttg cgcacaagca acaatgacct
    11641 tatccgccag taaaactttc gcaacaacag aatagcaaag aatcctatca tgcaggattc
    11701 ttgcagatcg tccgaattaa ttcgacctat ttgtaggcct gcgctatggc aagaaagtcc
    11761 ttggctttca acgatgcgcc gccgatcagt gcgccatcga cattggcgac cgccatcagc
    11821 gatgcggcat tgtcgggctt gaccgagccg ccatagagaa tgcgcatccg cccggccgtg
    11881 tcggcgccca gcgcgccctt cagggttttg cggatatcgg cgtgaacctc ggcaacatcg
    11941 gcttcggtcg gtgtcagccc ggtgccgatg gcccagaccg gttcataggc gatgatggta
    12001 ttccagcgat tggcgccgtc cggcagcgag ttcttcacct gcttgcggac caccgccagg
    12061 gtttttcccg cttcgcgctc ggcccgggtc tcgccaacgc agacgatggc gcgcagaccg
    12121 gcgcgcagcg cggcctcggc cttggctttc acgatggcat tggtctccgc atggtcggcg
    12181 cggcgctccg aatgaccgac gatgacaaag ctggcaccgg catcggccag catttcggcc
    12241 gagacatcgc cggtatgggc gccggacgcc ttcatgtggc aatcctggcc accgatttcc
    12301 agccgcgatc cgaggctggc ggcggcggcg gtcagcacca gcgtcgccgg cgtgcagatc
    12361 agcaacgcgg cccggttctg cagggcgaca tcatagcctt cggcgatggc cttgatctcg
    12421 gcaagggcgg cacgggtgcc gttcatcttc cagttgccgg cgacgagcgg gcgcggacgc
    12481 ttttgcatgg cgggttctct ttggagttga cgggatcgtt gcgcttcgcc ttaagccagt
    12541 tcaacacgcg cggcaattgg cggatggaaa tgatgttgcc gcagggtaaa acccccaggg
    12601 ggtcgaggcg agggcaaggc atggcggatc atcacacttc cacaccgcgc ggcatcgacc
    12661 atgccgtcat agccacgcgc gatctcgacg cgctcgccaa cagctttgcc gaactggggt
    12721 tcaaggtcgg tgcccgaaac cggcacccct ggggcaccga aaatcacatc gtgcagtttg
    12781 atggcagctt catcgaactg atcggcatcg gcgccggtgc cagcattgcc ggtcacgagc
    12841 cggggcggtt ctcgttcggc gggttcgtcc gcgactatct ggaggatgga gaaggcatgg
    12901 cgatgctggc gctggaaagc cacaacgcca ccggagaccg cgaggccttc cgcaaggcgc
    12961 gaatcggtga tttttcccgg ttcgattttg cccgttccgg tcagggcgcc gaaggcaagc
    13021 cggtcgatgt ggcgttttct ctggccttcg ccgagctttc cggatcgccc cgtgccgcct
    13081 ttttcgtctg ccagcagcac aagccgcaga atttctggtc aagggcggtg cagaaacacc
    13141 ccaatggagc caccggcatt gccagtgtcg ttgtggtggc cgacgatccg tccgacaccc
    13201 acgagtttct tggcgctttc gtcaaccagc gggaaatgcg cgcaacctcg ttcggactgg
    13261 acatcgacac cggtcgggga cgcatcgagg tgctgtcgcc ggatgccttt gcctaccgtt
    13321 tcggggcgcc ggccccggcc cggccaaagg gtggagcgca atttgctggc ctgacgatcg
    13381 ccggcgcctc ggtcgcccgc ctcgaaaaag tgacgcaggc tgctcgaaag gtcgtgacag
    13441 cacggcaaac catgctagtg acagagttgt ttgggggcgc ttgtcaactt ggttttgacg
    13501 tttgagcggg cgctatgaag gcgatagttt ttgtcgtgat tggcggcgtt gcggcgctgc
    13561 tgggttggct gtttttccag cccgaatgct ttcagggagt ggttgtggcc agcgagcacg
    13621 cctgcgtgca ggttcctggc tttgaccggc agttttgcga gcgtgccttc gcccgcccgg
    13681 aggaggccat ttttcgtgca ggcagcttct accagacaag agacgattgc ctgaggcggc
    13741 acaccgactg cattgagttc cccggtgttc atggctatac gccaaagccg accggttatt
    13801 gcgttttgcg cggggccgat ggcagccttg ctggcatgaa gccggtctac ggtcggccaa
    13861 gctagagcat gctgcgaaaa aatgtaggcg gttttttgct taaagcatgc tctagacact
    13921 agataacgat cacgctgtgt ttcggtgatt gaaccgaaac gagagcgtga tctaggaaga
    13981 agcaatggtg atgacatcca agtccgatgc gaacgaagtg cgcatcgggt ctctgaccct
    14041 gtcgaacgat ttgccgctga cgttgatagc tggcccctgc cagatggaaa gccgcgatca
    14101 tgcctttgcc gctgccgggt ttctcaagga catggcggat cggctgggca tcggcctcat
    14161 ctacaaatcc tcgttcgaca aggccaatcg cacatcgctg aatgcggcgc gcggcctcgg
    14221 tctggaaaag gcgcttggtg tctttgccga tctcaaggcc gccttcggct ttccggtcgt
    14281 taccgatgtg catgaggccg gccaatgcgc cgaggcggcg ggcgtcgtcg atgtgttgca
    14341 gatcccggcc tttctctgcc ggcagaccga ccttctggtc gcggcggcga aaaccggccg
    14401 tgtggtcaac gtcaagaagg ggcagttcct tgctccctgg gatatggcca atgtcgtcgc
    14461 caagctgcgc ggcgccggca atcccaatgt tctggtgacc gagcgcggtg tttcgttcgg
    14521 ctacaacgcc ctggtgtcgg acatgcgggc gctgccgatc atggccgaga cctgccgggc
    14581 gccggtgatc ttcgatgcga cccattcggt gcagcagccc ggcgggcagg gcggatcgtc
    14641 tggcggcgaa cgccgcttcg tggccgcgct ggcgcgggcg gctgttgcgg ttggggtggc
    14701 cggtgtgttc atcgaatgcc acgaggaccc cgatcatgcg ccctgcgacg gccccaacat
    14761 ggtggcgctg aaggatatgc cggcgctgat cgagcggctg atggcgttcg atcggctggc
    14821 caagggcctc tgatcccacc acgtcggacc tgccgcccgt tgccaagcac tgtgcttgcc
    14881 ctatacctct cgaactcctg cgccgacaag agattcgccc atggccgcaa caacgatcgc
    14941 tgtcattctt gctgccggtg aaggcacgcg gatgaaatcc ggtttgccca aggtgctgca
    15001 caaggtcgcc gggcgatctt tgcttggcca tgccctgttg ggagtgaagg acgccgggct
    15061 tgacggcgcc gccgtcgtga tcggacccaa ccacggcaag gtcgaggatg aagtccgttc
    15121 ggtgctttct ggcgccgatg tgttcgtgca ggccgagcgc cgcggcacgg cgcatgccgt
    15181 cttgcaggcc cgggcggcca tcgcgcgcgg ccacgcggcg attgtggtgc tgtttgccga
    15241 tacaccgctg gtccggtccg agacggtgcg gcggctggcg gatgcggtgc tgaagggggg
    15301 cgacatcggt gtgatgtgct ttcgcgccaa ggatccgacc ggctatggtc gcatcctgat
    15361 ggaaggcggc gagatcatcg ccattcgcga gcagaaggac gcgaccgagg ccgaacggcg
    15421 catcactctg tgttccggtg ggctgatgtc gttcaagggc gagcatgcct tacgcattct
    15481 cgatgcgatc gactgcaaca acgcgcagaa cgaatattac ctgactgcgg cggtcgaggt
    15541 ggcacggcgc atggggctga gggccgttcc ggtcgaggtg gcggaggagg aagcgttcgg
    15601 cgtcaacgac cgggcgcaac tggcctcggc cgaggcggtg gtgcagacgc ggctgcggcg
    15661 cgcggcgctg ctggcgggtg tgacgcttca agcccccggc acggtgtatt tctcggccga
    15721 cacccggctt ggctgcgatg tggtcatcga gccgaatgtc tatttcggcc ccggcgtgac
    15781 ggtcggggat ggcgtggtga tccatgcctt ttcgcatctt gagggcgccc atgtcggcaa
    15841 aggggcgagc gtcggtccgt tcgcccggct gcggcccggc gccacgctcg ccgccgaggt
    15901 caaggttggc aatttcgtcg aaatcaagaa tgccagtgtc gaggcgggcg ccaagatcag
    15961 ccacctgtcc tacatcggcg acgcccgcat cggcgccgag gccaacatcg gcgccggcac
    16021 catcacctgc aactacgatg gttatcgcaa gttcgtcacc gacatcgggg caggggcgtt
    16081 catcggctcc aattcctcgc tggtcgcgcc ggtgacgatc ggcgacggcg cctttgtcgg
    16141 ctccggctcg gtcgtcacca aggacgtgcc ggccgatgct ctggcggtgg cccgtggtca
    16201 ccagatggtc aaagccggct gggcgaggtc gttccgtgca cggcctgaga atgtcgccaa
    16261 ggcccggaag aagaaggact gatccggcgc ccgacagctt tcccttgact ctcgccggcg
    16321 tgcgacggaa agcgcaaggc agccacaatc gctccggacc tgccatgacc gcgctgccgc
    16381 atcacgaaac cgtcctgatc atcgatttcg gcagtcaggt gacgcaattg attgcgcggc
    16441 gggtgcgcga gtccggcgtc tattcggaag tgcatccgtt tcagtcggcc gaggcggcat
    16501 ttcgcgcgct gaagccgaag gcggtgatct tctcgggcgg ccccgacagc gtgacgcgcc
    16561 ccggcagccc gcgcgcgccg caggcggtat tcgactccgg cgtgccgatc ctcgccatct
    16621 gctatggcca gcagacgctc tgcacccagc tcggcggaac cgtcgagggc ggccatgcgg
    16681 cggagtttgg tcgcgccgat gtcgaggtga aagcgccctg cgcgctgttc gagggcatct
    16741 gggaggtcgg cgggcgctat cccgtgtgga tgagccacgg cgaccgcgtc actcgcctgc
    16801 cggccggctt caccgtcaag gcgcaatcgg aaaacgcgcc cttcgccgtc gcctccgacg
    16861 agaagcgccg ctactacacc accatgttcc accccgaggt ggtgcatacg cctgatgggg
    16921 cgaagctgat ctccaatttc gtccacagga tcgccgggct ggccggcgac tggacgatga
    16981 aggcgttccg cgccgagcag atcgaccgca tccgtgccca ggtcggcaag ggcagggtga
    17041 tctgcggcct ttccggcggc gtcgattcgg ctgtggcggc ggtgttgatc cacgaagcga
    17101 tcggtgatca gctcacctgc gtctttgtcg atcacgggct gatgcgccag ggcgaggcgg
    17161 gcgaggtggt gcggctgttc cgcgatcact acaacatccc gctggtccat gccgatgcct
    17221 cggagacttt cctcggcgcg ctggccggcg tgtccgatcc cgaggtcaag cgcaagacca
    17281 tcggcaagct gttcatcgat gtgttcgagg ccgaggcgaa aaaggtgggc ggcgccgggt
    17341 tcctggcgca gggcacgctc tatcctgacg tgatcgagag cgtctccttc accggcggcc
    17401 cgtcggtgac gatcaagtcg catcacaatg tcggcggcct gcccgagcgc atgaacatga
    17461 agctcgtcga gccgctgcgg atgctgttca aggacgaggt gcgggcgctg ggcctcgaac
    17521 tcggtctgcc cgagattttc gttggccgcc acccgttccc cgggccgggg ctggccatcc
    17581 gctgtcccgg cgagatcacg cccgaaaagc tcgatattct gcgcaaggcc gatgcgatct
    17641 atctggaaga gatccgcaat gccggcctct acgacaggat ctggcaggcc tttgccgtca
    17701 ttctccccgt gcgcacggtc ggcgtgatgg gcgacgggcg cacctacgat cacgttctgg
    17761 cattgcgggc ggtcacatcg gtcgatggca tgacggcgga tttcttcccc tatgacatgg
    17821 ctttcctcgg ccgcaccgcg acgcgcatca tcaacgaggt gcgcggcatc aaccgcgtcg
    17881 tctatgatgt aacctccaag ccgcccggca ccattgaatg ggagtagggc ggcttctggg
    17941 cggccgccgc aagggcattt ctgaacgaaa acgctcagcc cacaggcgct ttcggcattc
    18001 tgctgcgatc cgcatctgac agaccaaacg cctccgctga ccggaattcc cagcggatgg
    18061 cttctggcct gatgcgaccg tcgaaggtcc gttcggggat ttttctgccc tttatgcaga
    18121 ggatgatcgc gtcgtttccg ggcgggcaat gcagttcacg cacctccgcg ctggagccgt
    18181 cacgccacat cgcgaccgac cagttcggcg ggcgggccga tttgcccata actgtcgcct
    18241 gtttccgatt ctcgtcctgg gcccgctgga tcaggccgcg ggaggtaaag gtgagcacgg
    18301 acgatcccgg gtcctcggac agcccgagcg aatagtaccc cggccagcgc tgggcgatct
    18361 gcacgccgtc catcagcagc gcgatcacga tattcggccc gagactccgg atcgtttcat
    18421 ggcaagggtc ggttctggcc agatcctcac agatcaggac agcgaagctg gatttgttgc
    18481 ggaaggcgtg ggtgtagatc tgccgcctgc cgagttccat ggtctcccac cagtcggtgt
    18541 catcacagag tttgccggtt aggccatagg cgatgcactg gcgtggatcg atgcgccagc
    18601 gatggtgttt tccctgtgag atgtgttcta tgttcgccgg cggcgctgcc gatccgctcg
    18661 atccgcctgc cggggcccgg tagatattgc tgaccaacgc gaaattgccc ttgtcgtcgt
    18721 cacaattgtc gctggacccg gctatcacaa actcgatctc cggattggtg gaggcaagct
    18781 gttcggtcag atcgcggtgg agggctgttg tcagcgacag ttccgggaaa accacgccat
    18841 ggattgtcct gccggatttt tcggcgagca gggacacgaa cgcgccgagt tgctcggcgg
    18901 tcatgccctc tagccagcgc tgttcgagtt cgaagttccc ccatttcagc ggcgggtttg
    18961 tctcgaccgt tttggccgtc caccacccgg cctcgattcg gtaaggaaac gggatcagca
    19021 gcagattgag gtcttctccg tcgtcgctgc cggccgtggc aaatcgctgc catgcggtgt
    19081 tgatgcgtgt gaccggcggc agcagggcaa gatgcttgct gaacgtgcgc atggtgacgc
    19141 cgacctgcgc cgtgcgcgac ttcggctgca ccgccagcat gtccattgat gcgcgttggg
    19201 tgatggagaa tatgggcacc ggcgtgctgt gtgtcccgtg cgcgtggaaa aagtgtttcc
    19261 gaccacgggc gcgggcattg gtctcattaa aagcaacgat aacggcaact gtggttgggg
    19321 tgccgctgga gggaggggcg tcgacataac cagtattttc gcagacttca tcggcaatga
    19381 tcagcagctt gtaggcggcg atgacccatg ctggcagggt ctcgccagtc tttatccgca
    19441 cattgaactt cttggttttt ccgtcgtcga gcagctctgt ccaaagcgcc tgaacgcctg
    19501 ccggcacggt gctgagcctc tttttccatt cgtcggcaag tccaagcagg gcttcatggt
    19561 ccgcatgcgt cagggtgggg ttgaccgacc ctgctgggtc accgtggccg ggattgatgt
    19621 tctgatacgt gccggagcgt tcgagcagga aggcgcagac ggcgaaaaga tccggtggcc
    19681 agtcgggcgc cttcgaaaca tcggtgccgg caagatcgtc cgttccctct ggaaatgtgc
    19741 gccggatgat ttcgaacact gtaacgccgc cagatcgggc catagtagaa tcttctccgc
    19801 aatgccgcac cgcagaatca ggcatcgatt aaaattgagc cggcttccga cttcaagcga
    19861 atcgagcgca atggagccgc cctgaaccaa cattcgatgg aacaatgttg cggaaaggcg
    19921 gcaaagaggt aggaagatag gcctaggtat taattcagct attgacttcc ggaattttgt
    19981 cctacaacct ctgcgtcgtg tcgaaggtcg gggcaacggc ccgaggctag gcagtcgggg
    20041 gttgcaggtg ctgcatcgat gcactattta cctgctgatt acctgctgag cctatcctcc
    20101 cggggtcgaa tcgacggctc gcgggtgttt tggtgtgtct ccagcgttgc ctgaccgctt
    20161 ggcagacagg aaatgagtga acggcaatga cacaatggtc gaatgacgca gcgctcgaca
    20221 gctgggcaga actcggcaga aagctcgaac cggttctcag ggctcagctc ggtgacaagt
    20281 tcgatgcatg gacccggcgt atgaatgaaa atgtcgtcgc ttacgctccc gggttggatg
    20341 aatccagcgc ccaggaggtc cttgacgcca tgaacgcccc ctgggttccg gcgaagacgg
    20401 ccggcgggaa gcgctgaacc ggcctccgtt tgcgcggcct gagcgccctt gcgtggtact
    20461 ctccgctggg tcggggcatt ttcgcggtat ctgacaggtc ttccttctcc tgctggttga
    20521 acaggtcagc ggccagccgc cgcagcgatt tgttcgcacg gtcgacatca tggagcgcgc
    20581 gtccttactg cttgacggag agggcgtcga cagggggcgg gcttcccgca tggcgagacc
    20641 gccgatgccc ccccctccgc cccgcagccg gcaggttaac cctgcattcg caacactctt
    20701 gcaaccattc cttgacccac tgcatggcaa agaccggaaa actgggttag gatgccgtca
    20761 acctgaacgg attatggtgc cgttcgttca aatcgagcgg aaagtcgcgt ttgccgtctc
    20821 gacggagtga tgatgactga acggaatggg gcgaagctgg cggccgatcg caatggccgc
    20881 gcggctcgat cgttggatgg tgccgcgcac cgtctggcgc gtttctgcgt cgctggaatg
    20941 gttgggttgc tggttgccaa ttgcgccagc cagccgcagc gggctgcgcg cagcaccagc
    21001 aaggaggtcg gtgcctttgc cgattcctcc agatacggca acgccagccc gcgtgtcgtg
    21061 gccgaggggc aggatgtgcc caagggcggc ggcaagtatc atatcggcaa cccctaccgg
    21121 atcgccggac gcacctatta cccgtccgac cggcctgtcg gccataccca gtcgggcatg
    21181 gcctcgtggt atggcgaggc gttccatggc cgcaagaccg ccaacggcga ggtctatgat
    21241 cgtcgctcct attcggcggc gcatcccacc atgccgttgc cgagctatgc gcgcgtcacc
    21301 aacctgcgca acaaccattc ggtgatcgtc cgcgtcaatg atcgcggccc ctatcatggc
    21361 ggccgggtca tggatgtgtc ggagcgggtc gccaatgcgc tcgatttcaa gcataccggc
    21421 acgacccgcg tgcggatcga ctatcttggc aaggccgggc tggccggcag cgacgatgtc
    21481 cggctcgccg cctcgctgcg gacggacggt acgccggcct cctcgccagg c
//
LOCUS       DAOMXG010000022        21257 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_1497_length_21257_cov_9.566456, whole genome shotgun sequence.
ACCESSION   DAOMXG010000022 DAOMXG010000000
VERSION     DAOMXG010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 21257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21257
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1497_length_21257_cov_9.566456"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(252..1328)
                     /locus_tag="PLQ11_02810"
     CDS             complement(252..1328)
                     /locus_tag="PLQ11_02810"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011442505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HRK23862.1"
                     /translation="MARSNTYRLAVIAGDGIGKEVMPEGLRALEAAARKHKFSLDLDH
                     FGFSSWDYYEKHGKMLPDDWKAQIGGHDAIYFGAVGMPDKIPDHISLWGSLLLFRREF
                     DQYVNLRPVKLMPGVPAPLANRKPGDIDFLVVRENTEGEYSSIGGRMFAGTEREFVVQ
                     ESVMTRIGVDRILKYAFELARRRPRKKLTSATKSNGISITMPYWDERVAEMKKAFPDV
                     AVDQYHIDILTAHFVLNPDRFDVVVASNLFGDILSDLGPACTGTIGIAPSGNINPERR
                     FPSLFEPVHGSAPDIAGKGIANPIGQIWSAAMMLDHLGEREAAATIVRAIEEVLAKPA
                     YRTRDLGGPANTVECGKAVADAIA"
     gene            complement(1395..1883)
                     /locus_tag="PLQ11_02815"
     CDS             complement(1395..1883)
                     /locus_tag="PLQ11_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008839750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRK23863.1"
                     /translation="MKSADTPLDAIDARILAELQRDGRMTITDLAERVGLSASPCLRR
                     VRMLEDAGVIAGYVAVVDQNKVGLEVNVFASIRLERQREEELERFNAAVAQWPEVMEC
                     YLMTGTRDYLMRIVVGSLGDYERFIKEKLSRLDGVASIESSFALSRVKHATAVPIVQN
                     RR"
     gene            1995..4400
                     /locus_tag="PLQ11_02820"
     CDS             1995..4400
                     /locus_tag="PLQ11_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006201707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRK23864.1"
                     /translation="MDSRLPYLADIERRALWLAAWTIHNANHLRPAGEVKVGGHQASS
                     ASMATILTALYFGALRPEDRVAVKPHASPIFHALQYLAGNQTRAKLENFRGYKGAQSY
                     PSRTKDIDDVDFSTGSVGLGVAQTLFSSLVQDYVAAHGWLNGMEGKMISLVGDAEMDE
                     GNIYEALIEGWKHGLRNTWWIVDYNRQSLDAVVREGLWEKFEAMFSNFGWDVVIVKYG
                     QMQLSAFAEPGGAELKRWIDNCPNQLYSALTFQGGAAWRKRLNDDIGDQGAASALIAR
                     RSDSELAALMSNLGGHDLPSLVEAFEAARMHDRPTLFICYTVKGHNLPLAGHKDNHSG
                     LMTPTQMEEFRARSNVRPGHEWDKWEGSRLPAADLQRFVDASPFFAAGKRRLAAPRIA
                     VPEALATPAHGGKPVSTQMGFGQILNEIARSDSELAARIVTTSPDVTVSTNLGPWVNR
                     RGLFARQSMADTFKAERIPSTYTWEFSPEGQHLELGIAESNLFIALSAFGLSHSINGA
                     RLLPIGTVYDPFILRAADQLNYACYQDARFLLVATPSGVTLAPEGGAHQSIATPLVGM
                     AQDGLCAFEPAFVDELAVMMRFAFDYMQRDGEAEPDARNVSERTWLRDATGGSVYLRL
                     STRPLEQPARTMTSDLERDIIDGAYWMRKPGPNAEVVIAFTGALAPEAIEAVGLMGGD
                     RRDIGLLAVTSADRLTAGLSAAQRSREGGLVHAESHIERLLGTLPPHCVLVTVVDGYP
                     ATLSWLGAVHGHRTRALGVEHFGQTGTIADLYRHYGLDAQGILRAAQAITQGRPMRHL
                     RAL"
     gene            4495..5265
                     /locus_tag="PLQ11_02825"
     CDS             4495..5265
                     /locus_tag="PLQ11_02825"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HRK23865.1"
                     /translation="MTRDIAAQRHRAILARLETSGSVTVEELSRVLGVSRETIRRDLK
                     VLSVDGRLSIVHGGALHSESSEAPFTNRRTVNRAGKEAIARLAASLVGDGMTILLDSG
                     TTTEAVARALVRCGCRRLTVHTTSPANARIVSRLAGVRVYLIGGEFDRNEEASSGPEA
                     RRAIARLSADFAFASIGGVDEEGFLTDYTRTGATTRSALIKAAERGFLMADSSKFGRV
                     LPSRIAGDAQCAGLLVDRELPLPIAMALGARGIRVVIG"
     gene            5338..7764
                     /locus_tag="PLQ11_02830"
     CDS             5338..7764
                     /locus_tag="PLQ11_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014527147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRK23866.1"
                     /translation="MTFPTQARVVIIGGGIVGCSLAYHLTRLGWKDVLLLEQGRLSCG
                     TTWHAAGLVGQLRSHSSMTRLIRYSTELYASLEAETGLATGWKRCGSVSVARTPERMT
                     QLRRTISAARAQGVEIEELSPKEAGDKWPVMRTDDLVGGVWLPGDGKANPADITQALA
                     RGARNGGAVVREQVRVTAIEVENGRVKAVVTDQGRVDCEMLAICAGQWSREIGRMCGV
                     SVPLHSAEHMYVVTGRIDGVTPDLPVMRDPDGYIYFKEEVGGLVMGGFEPDAKPWGMN
                     GIPYPFEFQLLPDDWDQFAILMENALHRVPALETAEIKTFLNGPESFTADNNFILGEA
                     PEVAGVFVGAGFNSMGIASAGGAGRALAEWMVAGEPTSDLWPVDIRRFADFNNNPAWL
                     KDRVKEVLGLHYAMPWPNRELASARPFRRSPLYERLAAKGAVFGSRMGWERANYFARS
                     EAEKTIRYSFGPQNWLETVAAEHRACREAVGLVDMSSFAKYLVQGPDAEAALQHIAAN
                     DVAVPVGTSVYSALLNSRGTFESDLTIARIAPDAYLLLTGTAQATRDAHWIRRHLPGG
                     ASLTDVTSSYSVLALAGPRAAELLARVSPQNFDGDAFPANAIRKITIGYATTWACRRS
                     YLGDGFELYAPVEFTPAIYDTLHEAGSDLGLRDVGYYAVDSLRIEKGFRAWGRELTPD
                     VNPFEAGLGFAVALGKGNFIGREAAQAARKAPRTRRLIALVGPRPGDQMAWGGEAILA
                     DGRPVGEITSAAFGASLGGIVALGWAEAHEPIDQRWLDARSWAIDLAGTTVPVTASLA
                     APLDRKTPRG"
     gene            7923..8633
                     /locus_tag="PLQ11_02835"
     CDS             7923..8633
                     /locus_tag="PLQ11_02835"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRK23867.1"
                     /translation="MPQAAMSIVPATQEDNWTVRHASLFGRLPESVLSTLLDQQTVRV
                     TGKAVTLCEWGAPVETCFVVLKGMAKLVRSSAEGEAAVLSICGPGRTLMLAEGLSGKP
                     CSATVESVTPVRLMALDVAALRRSMATDSKLSMTLLAAAAADLRLLVGHVEELKAMTG
                     PVRVAAMILNLSEARAGARALILPYEKQLIAGRLGMAPENFSRALKQLREYGVQFSRD
                     RLQIADLGRLRAFLYERI"
     gene            complement(9057..10460)
                     /locus_tag="PLQ11_02840"
     CDS             complement(9057..10460)
                     /locus_tag="PLQ11_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007196679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HRK23868.1"
                     /translation="MNQHRLPRPDAGALGDVVMILSQRFGARASINDSLRDQHSHGEG
                     LPTIGKPDIVVFPESNEEVAFILSTCNDRRVPVVPFGAGSSLEGQLAALAGGVSVDMQ
                     RFDKVLDVNPDSMDCRVGAGVTREALNAHIRDTGLFFPVDPGANASIGGMAATRASGT
                     NAVRYGTMRENILGLTVVTPDGHIIRTGSRARKSSAGLDLTHLYVGSEGTLGIITEVQ
                     LKLYGVPESIVAATCQFDDLDSAVGAVVASLQAGLKLARIELLDEVQVKICNAYSSLG
                     LPETPHLFLEFVGSKSGTDEDVAFMKAIVADHGSGDFQFAERLEDRNRLWKARHNCYY
                     ACMAYAPGLKNMGTDACVPISALPQVLLETREDARRSGLITPIVGHVGDGNFHLGIMF
                     DENDPDQVEKAEGMAFRVARRAIAAGGTCSGEHGIGVHKIVHLEAEHGYGAEIMKQIK
                     RTLDPNGIMNPGKFYTP"
     gene            10628..11590
                     /locus_tag="PLQ11_02845"
     CDS             10628..11590
                     /locus_tag="PLQ11_02845"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006932795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HRK23869.1"
                     /translation="MTDGPLMFLVCGEALYDVFLRDEPGRGPVGLTAVIGGSPLNVAI
                     GLARQGERAGFFAGLSEDPLGARLAAHIAAEGVDGRYLCRKPNPTTLSLVSLGSGGVP
                     HYTFYGHAAADISLTLADLPVLGPEVTGVHAGSYTLVAQPVADAVAALLARERHRLIT
                     LDPNIRPTVEPDMAIWRRRIDALLPHVAVVKASDEDLDRLHPGEPPERIALRWLDQGP
                     AVVIITRGPNGAVALTRRLRLDCPAPPVAVVDTVGAGDTFQATLIAGLLAAGIRDRAG
                     IAGLGETDLAALLTRCCHAAAITCGRHGADLPSRDEIDRSQATG"
     gene            complement(11606..13024)
                     /locus_tag="PLQ11_02850"
     CDS             complement(11606..13024)
                     /locus_tag="PLQ11_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018262572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRK23870.1"
                     /translation="MDNRSDLWRRVDELKPRFIALSDRVWGMPEVCYTEVRSAAEHRA
                     ELVAQGFAVTDQLAGIPTAVMGEAGEEGPLVAILGEYDALPGLSQQAGLDHHQPIETG
                     GHGHGCGHNLLGSAALLAASAVKDWLKTHGVPGRVRYYGCPAEEGGAAKAFMVRDGAF
                     AGVDAAVTWHPSSWWEVMPAVSLANTRADFSFTGKASHAAAAPHLGRSALDAVELMNV
                     GVNYMREHMPSDARVHYALMDAGGIAPNVVQAHARVRYSIRAMDLPGMLDLVRRVRAI
                     AEGAALMTETRMQMAIISAVSNTVQNRTLEAALQTILEDLGPPHFDEADRAFARRIRA
                     TLSAGDIAAAWRAIGLDDTGADLCDFTVPAKTERKLQIGSTDVGDVSWAVPTVQAHAP
                     TLAIGTPLHTWQVVAQGKSPHAHKAMVQVAKAMAATAIAVLTDRSLREAAQAEHARFL
                     ARTPYVSPMPPEVRPPLTMSTG"
     gene            complement(13152..14156)
                     /locus_tag="PLQ11_02855"
     CDS             complement(13152..14156)
                     /locus_tag="PLQ11_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009492506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRK23871.1"
                     /translation="MTDPLIALDQASKTFERRIDLAGRIANVFGAGLKDIRLQAVDRV
                     DLSVSKGEIVGLVGESGCGKSTVGRLVAGIHSPSAGRVLWRGMDVASLDDAGRRACTL
                     GIQMIFQDPMASLNPRMRAGDIVAEAALVHGLFGADEREERVAAAFSRVGLDPSYARR
                     YPHQFSGGQRARIGIARALAVEPEVLVCDESVAALDVSIQAQVLNLFMDLGEQLGLSY
                     LFISHDLGVVRHIASRVIIMYLGRVVETARTEALFDAPAHPYTRALLDNVPRLDARHR
                     SFATIKGEIPSPINPPPGCHFHPRCPLVGPRCRAEVPALKAGPHGGTVACHLNDGGTA
                     "
     gene            complement(14153..15133)
                     /locus_tag="PLQ11_02860"
     CDS             complement(14153..15133)
                     /locus_tag="PLQ11_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRK23872.1"
                     /translation="MAEKVLEIAGLKTHFFTRAGVVKAVDGVSLSLARGEILGLVGES
                     GSGKSITGFSILGLIDPPGRIVEGSVRLMGQELVGRSDSQMQSVRGRDLAMIFQDPMM
                     TLNPVLSVGTQMVETVLAHEQVSKAEARARAVAALAMVGIPSPEERLDAYPHQFSGGM
                     RQRVAIAIALLHRPKVIIADEPTTALDVTIQSQILATVQELCRTTGTALIWVTHDLTV
                     IAGLADRIAVMYAGRIVETGPVDSVLDHPRHPYTIGLIGSVPSRGKRGERLAQIPGSA
                     PSLASLPQGCPFAPRCGKAIAACRSERPDLEPVGRSLVACHVAQGTGGAE"
     gene            complement(15136..16041)
                     /locus_tag="PLQ11_02865"
     CDS             complement(15136..16041)
                     /locus_tag="PLQ11_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008192460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRK23873.1"
                     /translation="MSAPAVAAFVEVETPLRRFVRQFSEDRVAVFGLVVFLLIVALAL
                     AAPFYAPQNPYDLSRVDLMDSRQPPGAKALDGYTMWLGSDGVGRDIFSAIFYGLRISL
                     AVGAASCLIAATLGMTVGLIAAYAGGRTETLIMRLVDLQLSLPSVLVALILVAMFGKG
                     VDKIILALVLVQWAYYARTVRGSALVERSKDYVEAARCLGLSHTRILFRHILPNCLAP
                     VIVVATVQTAHAISLEATLSFLGVGLPQTEPSLGVLIANGFQYMMSGKFWISTFPGLA
                     LLITIVAINLVGDRLRDVLNPRLEK"
     gene            complement(16038..17015)
                     /locus_tag="PLQ11_02870"
     CDS             complement(16038..17015)
                     /locus_tag="PLQ11_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008550673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRK23874.1"
                     /translation="MLAFILRRTGQGLLVIAAMIALVFFGVNVIGDPVWILVSPEMNQ
                     QQVEEVARSLGLDRPIYEQFWHFLKGLAQGDLGRSFVHGTPAIGLILERMPATLELAF
                     VALVMAIVLGIPLGLYAGLKPDHPLARSIMAVSILGFSLPTFWVGLMLIMVFAVMLGW
                     LPATGRGPVGHFLGIETSLTSLEGWRFVLLPALNLALLKISLVIRLARAGTREVALLD
                     YVKFARAKGLSPRRVIGVHILKNILIPIITVLGLEFGSLVAFSVVTETIFAWPGMGRL
                     LLEAIQRLDRPVIVAYVIVVVCLFVVINLVVDILYSLVDPRVRLTENAA"
     gene            complement(17073..18650)
                     /locus_tag="PLQ11_02875"
     CDS             complement(17073..18650)
                     /locus_tag="PLQ11_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014283364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRK23875.1"
                     /translation="MLRSHIAGAALATALLLGVPAQAQDIRIGVAAEPTSMDPHFHNL
                     TPNNQVMRHIFEPLVFQDEKQALKPGLAESWKTVDDTTWELKLRAGVKFHDGSDFGAD
                     DVLVTFKRAPDVPKSPAGFGSYIKGKTIEKVDDLTLRIKTAAPEPLMLSHLAQISIIS
                     RKNEAMTTEDYNSGKAAVGTGPFKFAEFVAGDRVVMVRNDSHWAGKPTWGKVTMRPVK
                     SDPTRVAALLSGDLDVIENVPTADAQRLKSDSRLATFSALSNRVIYFHLDRHREDSPF
                     IKGKDGSTIKNPLNDLKVRQALSKALNRTAIVERIMEGEAQPAAQFVPDSYGGTSKNL
                     KPVAYDLDGAKKLLADAGLPNGFKITIHGPNGRYTNDAKILEAAGQMWSRLGLDVSVE
                     SVPAANFFSRATTGGANGMPEFSLFLVGWSAGTGEPLDSARALTMTFDRAKGTGAANR
                     GRYSNPKADEILTKAFQTVDEAKRNALVAEGTELIINDVGIIPIHFPLNTWAAKKDYT
                     IIPRSDEYTLAMSMSRK"
     gene            complement(18852..19775)
                     /locus_tag="PLQ11_02880"
     CDS             complement(18852..19775)
                     /locus_tag="PLQ11_02880"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRK23876.1"
                     /translation="MSAVYSGVFPIAPTPFHPDGGLDLTSAERLFAHYAAIGSDGLTV
                     LGIMGEAPKLEPNESIAMLKVAVKAMAGKPIIVGVSAPGFAAMRSLAREAMEAGAAAV
                     MIAPPSHLRTDDSIVGYYADAVAAIGADVPFVIQDYPLVTSVQMTPGVIRRIVTDNPS
                     CVMLKAEDWPGLEKISTLRRWQADGSVRPLSIMTANGGLFLDFEMERGADGSNTGYAF
                     PEMLIEVVAHAKARRRDAAHDLFDAHLPLLRYEQQPGVGLAVRKYVMMRRGLIACDAQ
                     RKPGGSLSATARAEIDYLLGRLARTDPRASL"
     gene            complement(19792..20256)
                     /locus_tag="PLQ11_02885"
     CDS             complement(19792..20256)
                     /locus_tag="PLQ11_02885"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HRK23877.1"
                     /translation="MGWLELKIPPVAVWLIAAALVWLLDAATPGLRPAASPLWIAAAG
                     LLALTGAAAGIVGVLAFRAAQTTVHPMHPEQTSRMVTDGIYRWSRNPMYLGLALLLAA
                     LVLWWRAPAGLLVLAGFVVYMNRFQIMPEERALAAKFGAEFDRYAAKVRRWL"
     gene            complement(20263..20964)
                     /locus_tag="PLQ11_02890"
     CDS             complement(20263..20964)
                     /locus_tag="PLQ11_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease activity regulator RraA"
                     /protein_id="HRK23878.1"
                     /translation="MSLSPAVLAALKGVSTATITTILLKKGLRNVWMRGTRPLRPGQE
                     RLVGRAFTLRFVPAREDLATPESWSSPISTRAAIEAMPEGCIAVVDAMGIQDAGIFGD
                     ILCARMARRKVTALVSDGVVRDLDGVLGTGLPVWCRGAAAPPSVAGLTFVAWQQPIGC
                     GGVAVFPDDVIVADKDGAVVIPAALLDYVVEHGPEQERMEGWIMGEVDKGHPLPGLYP
                     MNAETKARYEATRKD"
     gene            complement(21016..>21257)
                     /locus_tag="PLQ11_02895"
     CDS             complement(21016..>21257)
                     /locus_tag="PLQ11_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23879.1"
                     /translation="IGSIPFRARRGDPAGRRAQSVSCDPVKTFFPQGARRTEIRRNGR
                     FRDKSSEKPLDRMPDALFGPFPVLVGKLLRRNGSI"
BASE COUNT         3819 a         6876 c         6827 g         3735 t
ORIGIN      
        1 cgccggcccg ccgcgatcct cgtagacccc ggacgaggcg cgatccgagg caacgatgat
       61 gccgattatg gcgtttgtca tggtccgtcg tgctcccatt gcagggctgg agtgattggc
      121 gctatcggtg cacctgaagc attttcaagc gaagcggatt ctggttcgca ttaagaaaat
      181 gcgaaaaaca aaaaaacaga gcacttttac ttgagttgga atgctctaag cggccgcgcg
      241 gggcaagccg gtcaggcaat cgcatccgcg actgccttgc cgcattcaac tgtattggcc
      301 gggccgccga gatcgcgggt gcgataggcc ggctttgcca gtacctcctc gatggcgcgg
      361 acgattgtgg cggccgcctc gcgctctccg agatggtcga gcatcatcgc cgccgaccag
      421 atctggccga tcgggttggc gattcccttg ccggcgatgt ccggtgccga gccatgcacc
      481 ggctcgaaca gcgagggaaa cctgcgctcc gggttgatgt tgcctgatgg cgcgatgccg
      541 atggtgccgg tgcaggcggg cccgagatcg gaaaggatgt cgccgaacag gttcgaggcg
      601 accacgacat cgaacctgtc agggttgagc acgaaatggg cggtgagaat gtcgatgtgg
      661 tattgatcga cggcgacgtc cgggaacgcc ttcttcatct ccgcgacgcg ctcgtcccaa
      721 tagggcatgg tgatggagat gccgttggat ttggtcgcgc tggtcagctt cttgcgcggg
      781 cggcgccggg cgagttcaaa ggcatacttc aggatgcggt caacgccgat gcgggtcatc
      841 accgattcct gaaccacaaa ctcgcgctcg gtgccggcga acatccggcc gccgatcgag
      901 gaatattcgc cctcggtgtt ctcgcgcacg accaagaagt cgatgtcgcc gggcttgcgg
      961 ttcgccagcg gtgccggcac gccgggcatc agcttgacgg gcctgaggtt cacatactgg
     1021 tcgaattcgc gccggaacag cagcagcgag ccccacagcg agatatggtc ggggatcttg
     1081 tccggcatgc cgacggcgcc gaagtagatc gcatcatgcc cgccgatctg cgctttccag
     1141 tcgtcgggca gcatcttgcc gtgcttctcg tagtagtccc acgaggaaaa gccgaaatga
     1201 tcgagatcga gcgagaactt gtgcttcctc gccgctgcct ccagagcgcg cagaccctcg
     1261 ggcatcactt ccttgccgat gccgtcgccg gcaatgacag caagacgata ggtgtttgaa
     1321 cgggccatgg tttgcctcat cgcgggatca gcgccgggtg cgcagcggtt tgtgcctgag
     1381 gtggcggggc agcgtcaacg ccggttttgc acaatcggca cggctgttgc gtgcttgacc
     1441 ctcgacagcg cgaagctcga ttcgatcgag gcaaccccgt caagccgcga cagcttttcc
     1501 ttgatgaagc gctcgtagtc gcccaggctg ccgaccacga tccgcatcag gtagtcgcgt
     1561 gtgccggtca tcaggtaaca ttccatcacc tccggccatt gcgccacggc ggcattgaac
     1621 cgttcaagtt cttcctcccg ctggcgttcg aggcggatcg aggcaaagac attgacctca
     1681 agccccacct tgttctggtc gaccaccgcc acatagccgg caatgacgcc ggcatcctcg
     1741 agcatgcgca cgcgcctgag gcacggcgag gccgacaatc ccacccgctc ggcaaggtcg
     1801 gtaatcgtca tccggccgtc gcgctgcaac tcggcaagaa tgcgcgcatc aatcgcatca
     1861 agcggcgtat cggcggattt catcaagaag tgctttgatc atagtgaagt atgccaaaaa
     1921 ctctgcatca tactgctcac tttggcaaga atcctgtcaa gcgtgcgcta gaatgcgcat
     1981 ctcaggagga agccatggat tcgcgtctgc cctatctcgc cgatatcgag cgccgcgcgc
     2041 tgtggctcgc cgcctggacg atccacaacg ccaatcatct ccgccccgcc ggcgaggtga
     2101 aggtgggcgg ccatcaggcc tcctcggcgt cgatggcaac catcctgacg gcgctgtatt
     2161 tcggcgcgct gcggccggag gatcgggttg cggtcaagcc gcatgcctcg ccgatttttc
     2221 atgcgcttca gtatctcgcc ggcaaccaga cccgcgccaa gctggagaac ttccgcggct
     2281 acaagggcgc acaaagctat cccagccgca ccaaggacat tgacgacgtg gatttctcca
     2341 ccggctcggt cgggcttggc gtggcacaaa ccttgttctc ctcactggtg caggattacg
     2401 tcgccgccca tggctggctg aacggcatgg agggcaagat gatctccctc gtcggcgatg
     2461 ccgagatgga tgagggcaac atctacgagg cgttgatcga aggctggaag catggcttgc
     2521 gcaacacctg gtggatcgtc gactacaacc gccagagcct cgacgccgtg gtgcgcgagg
     2581 gcctgtggga gaagttcgag gcgatgttct cgaatttcgg ctgggatgtg gtgatcgtca
     2641 aatacggaca gatgcagctt tccgccttcg ccgaaccggg cggtgccgaa ctgaagcgct
     2701 ggatcgacaa ttgccccaac cagctctatt cggcgctgac cttccagggc ggcgcagcct
     2761 ggcgcaagcg gctcaacgac gacattggcg atcagggcgc agcttcggcg ctgatcgccc
     2821 ggcgcagcga cagcgaactg gcggcgctga tgagcaacct cggcggccat gacctgccct
     2881 cgctggtgga agccttcgaa gcggcgcgga tgcatgatcg tccgaccttg ttcatctgtt
     2941 acaccgtcaa ggggcataac ctgccgctgg ccggccacaa ggacaaccac tccggcctga
     3001 tgacgccgac tcagatggaa gagttccgcg cccgttcgaa cgtgcggcct ggccatgaat
     3061 gggacaaatg ggagggcagt cgccttccgg cagccgattt gcagcgtttt gtcgatgcgt
     3121 cgccattctt cgccgccggc aaacggcgtc ttgccgcgcc gcgcattgcg gttcccgaag
     3181 cgctggcaac tcccgcccat ggcggcaagc ccgtctcgac ccagatgggc ttcggccaga
     3241 tcctcaacga gatcgcgcgc tccgatagcg agcttgccgc ccgcatcgtc accacctcgc
     3301 cggatgtgac cgtctccacc aatctcggcc cctgggtgaa ccggcgtggc ctgttcgccc
     3361 gccagagtat ggccgatacg ttcaaggccg agcgcattcc ctcgacctac acctgggaat
     3421 tctcgcctga gggccagcat ctcgaactcg ggattgccga atccaacctg ttcatcgccc
     3481 tgtcggcctt cggcctcagc cattcgatca acggcgcgcg gctgctgccg atcggcaccg
     3541 tctacgatcc gttcatcctg cgtgccgcag atcaactcaa ctacgcctgc tatcaggacg
     3601 cgcgcttcct gctggttgcg acgccttccg gcgttacgct tgcgccagaa ggcggggcgc
     3661 atcagtcgat cgccacgccg ctggtcggca tggcgcagga cggtctgtgt gcgttcgagc
     3721 ctgccttcgt cgatgaactc gccgtgatga tgcgctttgc ctttgactac atgcagcgcg
     3781 acggcgaggc cgaacctgat gcccgcaatg tcagtgaaag aacctggctg cgcgatgcca
     3841 ccgggggctc ggtctatctt cgcctctcca cccgcccgct ggagcagccg gcgcggacga
     3901 tgacgtcgga ccttgagcgc gacatcatcg acggcgccta ctggatgcgc aaaccggggc
     3961 cgaatgccga agtggtgatc gcctttaccg gtgcgctggc gcccgaggcc atcgaggctg
     4021 tcgggctgat gggcggggac cggcgcgaca tcggcctttt ggcagtgacc tcggcggacc
     4081 ggctgaccgc cggtctctcc gccgcccagc gctcccggga aggcggcctc gtccatgccg
     4141 aaagccatat cgagcggttg ctcggcaccc ttccgccgca ttgcgtgctg gtgaccgtgg
     4201 tcgatggcta tcccgccaca ttaagctggc tcggcgcggt gcatggccat cgtacccgcg
     4261 ccctcggcgt ggagcacttc ggccagaccg gcaccatcgc cgatctctac cggcactacg
     4321 gcctggatgc gcaaggcatc ctgcgggcgg cacaagccat cacccagggg cggccgatgc
     4381 gccacctcag ggccttgtga gggatttggg aggcacatcg tctgtggccg cagaatggtt
     4441 ccgtctgatc gcagcatgct ctaggctgga acacctttct ggcgatcccg ctccatgacc
     4501 cgtgacattg cagcccaacg gcaccgcgcc attctagcca ggcttgagac gtccggatcg
     4561 gtgacggtcg aggaactgtc gcgcgtgctg ggcgtctcgc gcgagacgat ccggcgcgat
     4621 ctcaaggtcc tgtcggtgga cggtcggctg tccatcgtcc atggcggtgc gctgcacagc
     4681 gaaagttctg aagccccttt caccaaccgt cgcactgtca atcgcgccgg caaggaagcc
     4741 atcgcccggc tcgccgcctc gctggtcggc gatggcatga ccattctgct cgattccggc
     4801 acgacgaccg aagccgtggc gcgcgccctg gtgcgctgcg gatgccggcg cctgaccgtc
     4861 catacgacct cgccagccaa tgcgcgcatt gtcagccggc tggccggtgt ccgcgtgtat
     4921 ctgatcggcg gcgaattcga ccgcaacgag gaggccagtt ccggccccga ggcccggcgg
     4981 gccatcgcgc ggctgtcggc cgatttcgcc ttcgccagca tcggcggcgt cgacgaggag
     5041 ggctttctga ccgattacac ccggacgggc gcaaccacac gcagcgcctt gatcaaggcg
     5101 gccgagcgcg gcttcctgat ggccgattcc agcaaattcg gccgcgtcct gccgagtcgc
     5161 atagcgggcg atgcgcaatg cgcggggctt ctggtcgatc gtgaactgcc ccttccgata
     5221 gccatggcac tcggcgcgcg cggtatccgg gtcgtaatcg gatagtttga ccgaatttgt
     5281 ggttttcatt gcttttctga cggaatgggg caccatgccc gcttgcagga ggatgccatg
     5341 acatttccca ctcaagcgcg cgtggtgatc atcggcggcg gcatcgttgg ctgctctctc
     5401 gcctaccatc tgaccaggct cggctggaag gacgtgctgc tgctggagca ggggcggctg
     5461 tcctgcggca cgacatggca tgcggccggt ctcgtcgggc agttgcgcag ccattcctcg
     5521 atgacgcggc tcatccgcta ttcgacggag ctttacgcct cgcttgaagc agaaaccggg
     5581 cttgccaccg gttggaaacg ctgcggctcg gtctcggtgg cgcgcacgcc ggagcggatg
     5641 acccagctgc gccgcaccat ttcggcggcc cgggcgcaag gcgtcgagat cgaggaattg
     5701 agcccgaagg aggccggcga caaatggccg gtgatgcgca ccgacgatct cgtcggcggc
     5761 gtctggctgc caggcgacgg caaggccaac ccggctgata tcacccaggc gctggcgcgc
     5821 ggagcgcgca acggcggggc ggtcgtgcga gaacaggtgc gcgtcaccgc gatcgaggta
     5881 gagaacggcc gggtgaaggc ggtcgtgacc gatcaggggc gggtggactg cgagatgctc
     5941 gccatttgcg ccggccaatg gtcgcgcgag atcggccgga tgtgcggcgt ctcggtgccg
     6001 ctgcattcgg ccgagcacat gtatgtcgtg accggcagga tcgacggtgt gacacccgat
     6061 ctgccggtga tgcgcgatcc tgatggttac atctatttca aggaggaggt cggcgggctg
     6121 gtgatgggcg gcttcgagcc ggacgccaag ccctggggca tgaacggcat tccctatcct
     6181 ttcgagttcc agttgctgcc tgacgattgg gaccagtttg ccattctgat ggaaaatgcg
     6241 ctgcaccgtg ttccggcgct ggaaaccgct gaaatcaaga ccttcctcaa cgggccggag
     6301 agtttcacag cggacaataa tttcatcctc ggcgaggcgc cggaagtcgc cggagtcttt
     6361 gtcggcgccg ggttcaattc gatgggcatc gcctcggccg gcggggccgg gcgggcgctg
     6421 gcggaatgga tggttgctgg cgagccgacc agcgatctct ggccggtcga catccgtcgc
     6481 tttgccgatt tcaacaacaa tccggcctgg ctgaaggatc gcgtcaagga ggttctcggc
     6541 ctgcactacg ccatgccctg gccgaaccgt gaactggcaa gcgcccggcc tttccgccgc
     6601 tcgccgctct atgaacggct ggctgcgaaa ggcgcagtgt tcggctcgcg catgggctgg
     6661 gagcgcgcca attatttcgc ccgctccgag gccgagaaaa ccatccgcta ttcgttcggg
     6721 ccgcagaact ggctcgaaac ggtagccgcc gaacatcgcg cctgccgcga ggcggtgggc
     6781 ctggtcgata tgagcagctt tgccaaatat ctggtgcagg ggcccgatgc tgaagcggct
     6841 ctgcagcaca ttgccgccaa cgatgtcgcc gttcccgtcg gcacctcggt ctattcggca
     6901 ctgctcaaca gtcgcggcac attcgaaagc gatctgacca tcgcccgcat tgcccccgat
     6961 gcctatctct tgcttaccgg taccgcgcag gcaacgcgcg atgcccattg gatccgccgc
     7021 catctgcccg gcggtgcaag cctgacggat gttacctcgt cttattccgt gctggcgctg
     7081 gcgggcccgc gcgcggcgga acttctggcg cgggtctcgc cgcagaactt cgacggtgat
     7141 gcctttccgg ccaacgccat ccgcaagatc accattggct atgccaccac ctgggcctgc
     7201 cgccggtctt atctcggcga tggtttcgag ctttatgcgc cggtcgagtt cacgccggcg
     7261 atctacgata cgctgcatga ggccggctcc gatctcggcc tccgggatgt cggctattac
     7321 gccgtggatt cgctgcgcat cgagaagggc ttccgcgcct ggggccgcga actgacaccg
     7381 gacgtcaacc cgttcgaggc ggggctcggc tttgccgtcg cgctcggcaa gggcaacttc
     7441 atcggccgtg aggccgcgca ggccgccagg aaggcgccgc gcacaaggcg gctaatcgcc
     7501 cttgtcggtc cacgcccggg cgatcagatg gcctggggcg gggaggccat tcttgccgat
     7561 ggcaggccgg tgggcgaaat cacctcggct gccttcggcg ccagcctcgg cggcatcgtt
     7621 gcgctcggct gggccgaggc gcacgaacca atcgatcagc gctggctcga tgcacgctcc
     7681 tgggccatcg atctggctgg aaccaccgtt ccggtcacag ccagcctcgc cgctccgctg
     7741 gaccgcaaga cgccgcgcgg ttgaaccgga cggatgcaag agcatgctgc gattaagtgg
     7801 aagccggtta atcgcgataa gcatgctcta aacacttgat aacgatcacg ttatctgtgt
     7861 tttgatggtt ctatcaaaac acagcgtgat ctaggcaacc ggtgagcaga aaagaggggc
     7921 gcatgccaca agcagccatg tccatcgtgc cagcgacaca ggaggacaat tggaccgtcc
     7981 gccatgcctc gctgttcggg cgattgcccg aatcggtgct ttccacgctg cttgaccagc
     8041 agacggttcg ggtcaccggc aaggcagtga ccctgtgcga atggggagcg ccggtcgaga
     8101 cctgctttgt cgtgctcaag ggaatggcca agctggtccg gtcatctgcg gaaggcgagg
     8161 cggccgtgct gtcgatctgc ggccccggcc gcacgctgat gctcgcagag ggtctttccg
     8221 gcaaaccttg ttcagccacc gtggaatcgg tgaccccggt gcgcctgatg gcgctcgatg
     8281 tagcggcgct gcgccggagc atggcaaccg acagcaaatt gtcgatgacc ctgctcgctg
     8341 cggctgcggc cgatttgcgg ctgctggtcg ggcatgttga agaactcaag gcgatgaccg
     8401 gcccggtccg cgttgcagcg atgatcctga acctgtcgga agcgcgcgcc ggcgcccggg
     8461 cgctgatcct gccgtatgag aagcagctga tcgccggccg cctcggcatg gcaccggaga
     8521 acttctcccg tgccctgaaa cagctcaggg aatacggcgt ccagttctcc cgcgaccgct
     8581 tgcagatcgc cgatctgggg cgtttgcggg cgtttctgta tgagcggatc tgatcaacca
     8641 tcgtctgtcg caacgttttg aacaagtccg tcgtgcgagt ggacagcggc tggatcggtg
     8701 ataggatgcg acggaagttc agcgacaatt tgtcgcaata acgaagccca tgggccgcag
     8761 cttgccggaa tggtcgagct ggttgttgtc cgatgctttg gagcgggagc gcttgaaatg
     8821 gtgcatgcaa gagccggtgc aatgcgtttt gatccggaat tctgcccatt gctcaagggg
     8881 ctctcgcctg aggagaagcg ggctcgcatg gagagcgatc cgctgatacg ggcctgcatc
     8941 cagtcggtag agaccggaaa cccctacccg gttgagtgta accctcaccg accggctgcc
     9001 gccggccttg cccgtccgcg caaaggctcg cccaggccgc gtcgcgctgc catcgctcag
     9061 ggcgtgtaga attttcccgg gttcatgatc ccgtttgggt cgagcgtgcg tttgatctgt
     9121 ttcatgatct cggcgccata gccgtgttcg gcttcgagat ggacgatctt gtgcacgccg
     9181 atgccgtgct cgcccgagca tgtgccgccg gcggcgatgg cccggcgggc gacgcggaac
     9241 gccatgccct ccgctttttc gacctggtcc ggatcgttct catcaaacat gatgccgagg
     9301 tggaaattgc cgtcgccgac atggccgacg atcggcgtga tcagcccgga gcggcgtgca
     9361 tcctccctgg tttccagcag cacctgtggc agcgccgaga tcggcacgca ggcatcggtg
     9421 cccatgtttt tcaggccggg cgcataggcc atgcaggcat agtaacaatt gtgccgcgcc
     9481 ttccacagcc ggttgcgatc ctccagccgt tcggcgaact ggaaatcgcc gctgccgtga
     9541 tcggcgacaa tcgccttcat gaaggcgaca tcctcgtcgg tgccggattt cgagccgacg
     9601 aattcgagga acagatgcgg tgtctccggc aggccgaggc tggaataggc gttgcagatt
     9661 ttgacctgca cctcgtcgag gagttcgatg cgagcgagtt tcaacccggc ctgcaacgag
     9721 gcgaccaccg cgccgaccgc gctgtcgaga tcgtcgaact ggcaggtggc cgcaacgatg
     9781 ctctccggca cgccgtaaag tttgagctga acctcggtga tgatgccgag cgtgccttcc
     9841 gagccgacat aaagatgggt caggtcgagg ccggcggagg atttgcgggc gcggctgccg
     9901 gtgcggatga tgtgcccgtc gggcgttacc acggtcagcc cgaggatgtt ttcacgcatc
     9961 gtgccatagc ggacggcatt ggtgccggag gcacgcgttg cggccatgcc gccgatcgag
    10021 gcattggcgc cggggtcgac cggaaagaac agaccggtat cgcggatatg cgcgttcagc
    10081 gcctcacgcg tcacaccggc gccgacccgg caatccatcg agtccggatt gacatccaga
    10141 accttgtcga agcgctgcat gtcgacgctc accccgcccg ccagcgcggc aagctgacct
    10201 tccagcgacg accccgcgcc gaacggcacc accggcaccc tgcgatcgtt gcaggtcgac
    10261 aggatgaacg cgacctcctc gttgctctcc ggaaacacca cgatgtcggg ttttccgatg
    10321 gtgggcagcc cctcgccgtg gctgtgctgg tcgcgcaggc tgtcattgat cgaggcccgt
    10381 gctccgaaac gctggcttag gatcatcacg acatcgccaa gagcgccggc atcagggcgg
    10441 ggaaggcggt gctggttcat gctcgggctc gaaggggcgg cagggttgga caaacacgtc
    10501 cccgagccgt gggatcatcc ggcggcgcag gggcacatca aactaccgcc tggcatgttc
    10561 cgatcaagtg gatgccgtgc ttgcgccgga gggtggattt ggacaggatc gcacccgcgg
    10621 ccaatcaatg accgatggac ctctgatgtt tctggtgtgc ggcgaagcgc tctacgatgt
    10681 gttcctgcgc gatgagcccg ggcgtggacc agtcgggctc acggcggtga tcggcggatc
    10741 gccgctcaat gtggcgatcg gtctggcgcg gcagggcgaa cgcgccggct ttttcgccgg
    10801 cctgtcggag gatccgcttg gtgcccgcct tgcggctcat atcgccgccg agggggtcga
    10861 tggtcgctat ctctgccgca agccgaaccc gacgacgctc agtctcgtca gcctcggttc
    10921 tggaggcgtg ccgcattaca ctttttatgg ccatgcggcg gccgatatca gcctgactct
    10981 ggcggatctg ccggtgctcg gccccgaggt taccggggtg catgccggct cctatacgct
    11041 ggtggcgcaa ccggtggccg atgcggttgc cgcattgctg gcgcgcgaac gccatcggct
    11101 cataacgctc gatcccaaca tccgcccaac cgtcgagccg gacatggcga tctggcgccg
    11161 gcgcattgac gcgctgctgc cgcatgtcgc tgttgtgaaa gcctccgatg aggatcttga
    11221 ccggttgcac cccggagagc cgccggagag aatcgctctc cgctggctcg atcaggggcc
    11281 ggctgtggtg atcatcacgc gcgggccaaa tggcgccgtg gcattgaccc gcaggctgcg
    11341 gctggactgc ccggcgccgc cggtcgcagt ggtcgatacg gtcggcgccg gcgacacctt
    11401 tcaggcgacg ctgatcgccg ggttgctggc tgcaggaatc cgtgaccgtg ccggcatcgc
    11461 tggccttggc gagaccgatc ttgccgccct gctgacccgc tgctgccacg ccgccgcgat
    11521 cacctgcggg cgccatggcg cggatcttcc atcgcgggac gagattgacc ggagccaggc
    11581 cacagggtga ggtgctgcga tctggtcagc cggttgacat ggtcaacggc ggccggacct
    11641 ccggcggcat cggcgagaca tagggtgtgc gcgcaaggaa gcgggcatgt tctgcctgcg
    11701 ccgcctcacg caacgagcga tcggtgagaa ccgcgatggc cgtcgccgcc atcgccttgg
    11761 cgacctggac catcgccttg tgggcatgcg gcgacttgcc ctgcgccacc acctgccagg
    11821 tatggagcgg tgtgccgatg gccagcgtcg gcgcatgcgc ctgcacggtc ggcacggccc
    11881 aggacacgtc gccgacatcg gtggagccga tctggagctt tcgctccgtc ttggccggga
    11941 cggtgaaatc gcacaggtcg gcgccggtat cgtcaaggcc gatcgcccgc caggctgccg
    12001 caatatcgcc ggcgctcagt gtggcgcgga tgcggcgggc gaaggcgcgg tctgcctcgt
    12061 cgaaatgcgg cggtcccaga tcctcaagga tcgtctgcag ggcggcctcc agcgtccggt
    12121 tctggaccgt gttggacacg gcgctgatga tcgccatctg catcctggtc tcggtcatca
    12181 gcgccgcgcc ctcggcgatg gcgcgcaccc ggcggacgag atcgagcatg cccggcaggt
    12241 ccatggcgcg gatcgaatag cgcacgcggg catgggcctg gacgacattg ggcgcgatgc
    12301 cgccggcatc catcagcgca taatggacgc gcgcatcgct cggcatgtgc tcacgcatgt
    12361 aattgacgcc gacattcatc agttccaccg catcgagcgc gctgcgcccg agatgcggcg
    12421 cggcggcggc gtgcgaggcc ttgccggtga aggaaaaatc ggcgcgggta ttggccagcg
    12481 acaccgccgg catcacttcc caccagctcg aggggtgcca ggtgacggcg gcgtccacgc
    12541 ccgcaaaagc accatcgcgc accatgaacg ccttggccgc gcctccttcc tcggcggggc
    12601 agccataata gcgcacgcgg cccggaaccc cgtgggtctt gagccagtcc ttgacggcgg
    12661 aagctgccag cagggccgcc gaacccagca gattgtgacc gcaaccatga ccatggccgc
    12721 cggtctcgat cggttggtgg tggtcgagcc cggcttgctg gctgagaccg ggcagggcat
    12781 catactcgcc gagaatggcg accagcggcc cttcctcgcc ggcctccccc atgaccgcgg
    12841 tcggaatgcc ggcgagctga tcggttacgg caaagccttg cgcaaccagt tccgcccggt
    12901 gctcggccgc cgaacgcacc tcggtatagc agacctcggg cataccccag acgcggtcgc
    12961 tgagcgcgat gaagcgcggc ttcagttcat cgacacggcg ccagaggtcg ctgcggttgt
    13021 ccatgtcatg cctcctagag cattttcgag cgaagtggtt tccggttcgc gttaagaaaa
    13081 tgcgaaaaaa caaaaagata gagcgctttt tgggtgaatt ggaattcgct gaaagcgctc
    13141 tggaatgaaa cctatgccgt gccgccatca ttgagatggc aggcgacggt gccgccatgt
    13201 ggtccggcct tcagggccgg cacttcggcg cggcagcgcg gacccaccaa tgggcagcgc
    13261 ggatggaaat ggcagcccgg cggcggattg atcggcgaag ggatttctcc cttgatcgtc
    13321 gcaaagctgc gatggcgcgc gtcgagccgc ggcacattgt cgagcagggc gcgggtatag
    13381 ggatgggcag gagcgtcgaa cagcgcttca gtccgcgccg tttcgaccac gcggccgaga
    13441 tacatgatga tgacgcggct ggcgatgtga cgcaccacgc cgaggtcatg gctgatgaac
    13501 aggtagctga ggccaagctg ctcgccgaga tccatgaaca ggttgagcac ctgcgcctgg
    13561 atcgacacat caagcgcggc gaccgattca tcgcagacca gcacctccgg ctcgaccgcc
    13621 agcgcccggg cgatgccgat gcgggcgcgc tggccgccgg agaactggtg cggatagcga
    13681 cgggcatagg aagggtcgag gccgacgcgg ctgaaggcgg cagccacgcg ctcctcgcgc
    13741 tcgtcagcgc cgaacagacc atggaccagt gccgcctcgg ccacgatgtc gccggcccgc
    13801 atgcgcggat tgagcgaggc catcggatcc tggaagatca tctggatacc aagcgtgcag
    13861 gcacgccgtc ccgcatcatc gaggcttgcc acatccatgc cgcgccacag gacacgtccg
    13921 gcactcggcg agtgaatgcc ggcgaccagc cgcccgaccg tggattttcc gcagccggat
    13981 tcacccacca aaccaacgat ttcacccttg ctgacgctga gatcgaccct gtccacggcc
    14041 tgaagccgga tatccttgag cccggcgccg aacacgttgg cgatacggcc ggccagatcg
    14101 atgcgccgct cgaacgtctt gctggcctga tcgagggcaa tcagcgggtc cgtcattcgg
    14161 cgcctcccgt gccttgcgcc acatggcagg cgaccagcga gcgtccgacc ggttcgagat
    14221 caggcctctc gctccggcac gcggcgatcg ccttgccgca gcggggggca aacgggcagc
    14281 cttgcggcag gctggccagc gatggcgccg aacccggaat ctgcgcaagc ctttcgcccc
    14341 gtttgccgcg acttggcact gagccgatca gcccgatcgt gtagggatgg cgcggatgat
    14401 cgagcacact gtcaaccggc ccggtctcga cgatgcggcc ggcatacatc accgcgatcc
    14461 ggtcggcgag gccggcgatg acggtcagat catgcgtcac ccagatcagc gccgtgccgg
    14521 tggtgcggca cagctcctgg accgtcgcga gaatctggct ctggatcgtc acatcgagcg
    14581 ccgtggtcgg ctcatccgcg atgatcacct tgggccggtg cagcagcgcg atggcgatgg
    14641 cgacgcgctg gcgcatgccg ccggagaact gatgcggata ggcatccagc cgctcttccg
    14701 gcgagggaat gccgaccatc gccagtgccg cgacggcgcg cgcccgcgcc tcggccttgg
    14761 acacctgctc gtgcgccaga acggtctcga ccatctgcgt tccgacgctg agcaccggat
    14821 tgagcgtcat catcgggtcc tggaagatca tcgccagatc gcggccgcgc accgactgca
    14881 tctggctgtc gctgcgcccg acaagctcct ggcccatgag ccgaaccgaa ccctcgacga
    14941 tgcgccccgg cggatcaatc agaccgagaa tggaaaaccc ggtgatcgac ttgcccgatc
    15001 ccgattcgcc gaccagcccg aggatttccc cgcgcgccag cgacagcgac accccgtcga
    15061 ccgccttgac cacgccggcg cgggtgaaga agtgggtttt cagcccggcg atttcgagca
    15121 ccttctccgc catggtcatt tctcgaggcg ggggttgagc acgtcgcgca gccggtcgcc
    15181 gacgaggttg atggcgacga tggtgatcag cagcgcaagg ccgggaaagg tcgagatcca
    15241 gaacttgccc gacatcatgt attgaaagcc attggcgatc agcacgccga gcgatggctc
    15301 tgtctgcggc aggccgacgc cgaggaacga cagcgttgcc tccagcgaga ttgcatgggc
    15361 ggtctgcacc gtcgcgacca cgatcaccgg cgccaggcaa ttgggcagga tgtggcggaa
    15421 caggatgcgg gtgtgcgaca ggccaaggca gcgcgccgcc tcgacataat ccttcgaccg
    15481 ttcgaccagc gccgagccac gcaccgtgcg cgcataatag gcccattgca ccagcaccag
    15541 cgccagaatg atcttgtcga cacccttgcc gaacatcgcc acgaggatga gcgccacaag
    15601 cactgaaggc agcgacaatt gcagatcgac cagccgcatg atcagcgttt ccgtccgccc
    15661 gccggcatag gcggcgatca gcccgaccgt catgcccaaa gtcgccgcga tcagacagct
    15721 tgccgcgccg acggccagtg agatgcgcaa cccgtagaag atggcagaga agatgtcgcg
    15781 accgaccccg tccgatccca gccacatcgt atagccgtcg agcgcctttg cacccggcgg
    15841 ctggcgcgaa tccatcagat cgacgcggga caggtcatag gggttctgcg gcgcatagaa
    15901 cggcgcggca agggccagcg cgacgatcaa aagaaagacc accagcccga ataccgccac
    15961 gcgatcctcg gagaactggc gcacgaaccg cctgagcggc gtttcaacct cgacaaaagc
    16021 ggcaacggcg ggggcgctca tgcggcattc tccgtcagcc gcacgcgcgg atcgaccagc
    16081 gaatagagga tgtccacgac caggttgatg accacgaaca ggcacaccac gacgatgacg
    16141 taagccacga tcaccggccg atcgagccgc tggatcgctt ccagcagcag ccggcccatg
    16201 cccggccagg caaagatcgt ctcggtcacc accgaaaacg ccaccagcga gccgaattcc
    16261 agccccagca cggtgatgat cgggatcagg atgttcttga ggatatgcac gccgatcacc
    16321 cggcgcggcg acaagccctt ggcgcgggcg aatttcacat aatcgagcag cgccacctcg
    16381 cgcgtgccgg cacgggccag gcggatcacc agcgagattt tcagaagcgc cagattgagc
    16441 gccggcaaca gcacgaaccg ccaaccttcc agcgaggtca acgatgtctc gatgccaaga
    16501 aaatggccga ccggcccgcg cccggtcgcc ggcagccagc cgagcatcac cgcgaacacc
    16561 atgatcagca tcagcccgac ccagaaggtc ggcagcgaaa acccgagaat cgacaccgcc
    16621 atgatgctgc gggccagcgg atgatccggc ttgagaccgg catagagccc cagcggaatg
    16681 ccgagcacga tcgccatcac cagcgcgacg aatgccagtt ccagcgtcgc cggcatgcgt
    16741 tcaagaatga ggccgatggc cggtgttcca tgcacgaacg accggccgag atcgccctgc
    16801 gccagcccct tgaggaaatg ccagaactgt tcgtagatcg gccgatccag ccccagcgag
    16861 cgggcgactt cctccacctg ctgctggttc atctccgggc tgaccaggat ccacaccgga
    16921 tcgccgatca cgttcacgcc gaaaaacacc agcgcgatca tcgccgcaat gaccagcagc
    16981 ccctgaccgg tgcgccgcag aatgaaggca agcatggggt tcgcctcttg atccggcgcg
    17041 cggcaccatc ggtgccgcgc gtcagccctg acttatttcc ggctcatcga catcgccagc
    17101 gtgtattcgt ccgaccgcgg gatgatcgtg tagtccttct tcgccgccca ggtgttgagc
    17161 gggaaatgga tcgggatgat gccgacatcg ttgatgatga gttcggtgcc ttccgcgaca
    17221 agcgcgttgc gcttggcttc atcgacggtc tggaatgcct tggtcaggat ttcatccgct
    17281 ttcgggttgg aataccggcc gcgattggcc gctccggtgc ccttggcccg gtcgaaggtc
    17341 atggtcagtg cccgcgccga atcgagcggt tcaccggttc cggcgctcca gcccacgagg
    17401 aacagcgaga attccggcat tccgttggcc ccgcctgttg tggcgcgcga gaagaaattc
    17461 gccgccggaa ccgactccac cgagacatcg agcccgaggc gcgaccacat ctggccggca
    17521 gcttcgagga tcttggcatc gttggtgtag cggccgttgg gtccatggat ggtgatcttg
    17581 aagccgttcg gcaagccggc atcggccagc agcttcttgg cgccatcaag atcgtaggca
    17641 accggcttca ggttcttcga cgtgccgcca tagctgtccg gcacgaattg cgcggccggc
    17701 tgggcctcgc cttccatgat gcgttcgacg atcgctgtcc ggttgagcgc cttcgacagc
    17761 gcctgccgca ccttgagatc gttcagcggg ttcttgatcg tcgatccgtc cttgcccttg
    17821 atgaacgggg agtcctcgcg gtgccggtcc aggtggaaat agatgacccg gttggacagc
    17881 gcgctgaacg tggccagccg actgtcggat ttcagtcgct gggcgtcggc ggtcggaacg
    17941 ttctcgatca cgtcgaggtc gcccgacagc agcgccgcga cgcgcgtcgg atcggacttg
    18001 accggccgca tggtcacctt gccccaggtc ggcttgccgg cccagtggct gtcattgcgc
    18061 accatcacga cacgatcgcc tgcgacaaat tccgcgaact tgaagggccc ggtccccacg
    18121 gcggccttgc cggaattgta gtcctcggtg gtcatcgcct cgttcttgcg cgagatgatg
    18181 gagatctggg cgagatggct cagcatcagc ggctcgggcg ctgccgtctt gatgcgcagg
    18241 gtcaggtcat cgaccttctc gatggtcttg cccttgatgt acgagccaaa gccggccggg
    18301 ctcttgggca catccggcgc gcgcttgaag gtgacgagca catcgtcggc gccgaaatcc
    18361 gatccgtcat ggaatttcac gccggcgcgc agcttgagtt cccaggtcgt gtcatcaacg
    18421 gtcttccagc tttccgccag ccccggcttg agcgcctgct tctcgtcctg gaacaccagc
    18481 ggctcgaaga tatgccgcat gacctgattg ttcggcgtca ggttgtgaaa atgcggatcc
    18541 atcgacgttg gttcggcggc aacaccgatc cggatgtcct gtgcctgcgc cggcacaccc
    18601 agcaacaacg cggtggcgag cgcggcgccc gcaatatggc ttcgcaacat tggtctctcc
    18661 cgtctggatg cgcagcctaa cccggcccga tcggggggtg ctgcaccaat cattcaaaac
    18721 gcaggccgcc tccgggccgc gtctccctgc cgcgcaccgt gcgagcgatt gcaggagaaa
    18781 tcaagtgcaa gaagcatcaa gaaaactcag gcggtgacaa cgcggatggc tcttgccgtg
    18841 gatcgatgtc gtcacaggct tgcgcgcgga tcggtcctcg caaggcgtcc gagcaggtaa
    18901 tcaatctcgg cgcgcgccgt tgccgacagg ctgccgccgg gcttgcgctg ggcatcgcag
    18961 gcgatcagcc cgcgccgcat catcacatat ttgcgcaccg ccagccccac gcccggctgc
    19021 tgctcatagc gcagcagcgg cagatgcgcg tcgaaaagat catgcgccgc atcgcgtcgc
    19081 ctagcctttg catgggccac gacctcgatc agcatttccg ggaaggcata gccggtgttg
    19141 gagccgtcag cgccccgctc catctcgaaa tcgagaaaca gtccaccatt ggcggtcatg
    19201 atcgaaaggg ggcgaaccga gccatccgcc tgccatctgc gcaaggtgga aatcttctca
    19261 aggcccggcc aatcctcggc cttcagcatc acgcaggagg gattgtcggt gacgatgcga
    19321 cggatgacgc ccggcgtcat ctgcaccgat gtcaccagtg gataatcctg gatgacgaac
    19381 ggcacgtcag cgccgatcgc ggcgaccgca tcggcgtaat agccgacgat ggaatcatcc
    19441 gtccgcaggt gggacggcgg ggcgatcatg accgcggccg cgcccgcttc catcgcctcg
    19501 cgcgccagtg accgcatggc cgcgaagccg ggcgccgaca caccgacgat gatcggtttg
    19561 ccggccatgg ccttcaccgc gaccttcagc atggcgatcg attcattcgg ctccagcttc
    19621 ggcgcctcgc ccatgatgcc gaggacggtc aggccatccg agccgatggc ggcataatgg
    19681 gcaaacagcc tctccgcgct ggtcaggtcc aacccaccat ccgggtgaaa cggcgtcgga
    19741 gcgattggaa acacaccgga atagacagcg ctcatgaaaa cctcgttagg gtcacagcca
    19801 gcgcctgacc tttgcagcat agcggtcgaa ttccgccccg aacttggcgg ccagtgcccg
    19861 ttcctccggc atgatctgga acctgttcat gtaaacgacg aagccagcca gcaccagcag
    19921 ccccgccgga gcacgccacc atagaaccag cgcagccagc agcagcgcaa gtcccagata
    19981 catcgggttg cggctccaac ggtagatgcc gtccgtcacc atccggcttg tctgctccgg
    20041 atgcatcgga tgcaccgttg tctgtgccgc acggaaagca agaacgccga cgattccggc
    20101 tgcggctccg gtgagggcca gcagcccggc cgcagcgatc cacagcggtg aggccgccgg
    20161 ccgcaacccc ggcgttgccg cgtcgagcag ccagaccagc gcggccgcga tcagccagac
    20221 cgcgacggga ggaattttca gctccagcca gcccacggga ctctagtcct tgcgcgtcgc
    20281 ttcgtagcgc gccttggtct cggcattcat cggatagagg ccgggcaggg gatggccctt
    20341 gtccacctcg cccatgatcc agccttccat gcgctcctgt tccggcccgt gctccacgac
    20401 ataatcgaga agcgcggcgg gaatgaccac cgcgccatcc ttgtcggcca cgatgacatc
    20461 atcaggaaac accgcaaccc cgccgcagcc gatcggctgc tgccaggcga caaaggtcag
    20521 gccggcaacc gacggcggcg ccgccgcacc ccggcaccac accggcaggc cggtgccgag
    20581 cacgccgtca aggtctcgga ctacgccgtc gctcaccagc gccgtgacct tgcgtcgcgc
    20641 catgcgcgcg caaaggatat cgccgaaaat gccggcatcc tgaatgccca tcgcatcgac
    20701 cacggcaatg cagccttccg gcattgcctc gatggcggcg cgggtggaaa tcggcgatga
    20761 ccagctttca ggcgtggcaa gatcctcgcg tgccggcacg aaccgcaaag tgaaggccct
    20821 gccgacaagg cgctcctgcc ccgggcgcaa cgggcgtgtg ccgcgcatcc aaacattgcg
    20881 caggcccttc ttcagcagga tcgtggtgat tgtagcggtg gaaacgccct tgagcgcagc
    20941 aaggacagcg ggcgaaagcg acatggcaga tccggacagg gttacgactg gcccgaagct
    21001 tagagcaata tccgatcaga ttgaaccatt ccgacggagc aatttcccga caaggaccgg
    21061 gaaaggcccg aagagcgcat ctggcatacg gtcgaggggt ttttccgagg atttgtcgcg
    21121 aaatcgcccg ttcctccgga tttccgtgcg gcgggcgccc tgcgggaaga aggtcttgac
    21181 cgggtcgcag gacacgctct gcgcccttct tcccgcagga tcgccgcgcc gcgcgcgaaa
    21241 cggaattgat ccgatct
//
LOCUS       DAOMXG010000023        20711 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_1542_length_20711_cov_10.130809, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000023 DAOMXG010000000
VERSION     DAOMXG010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 20711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20711
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1542_length_20711_cov_10.130809"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /gene="gcvT"
                     /locus_tag="PLQ11_02900"
     CDS             complement(<1..897)
                     /gene="gcvT"
                     /locus_tag="PLQ11_02900"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HRK23880.1"
                     /translation="MAGADSPQMPLRTPLYDAHVAAGARMVPFAGYDMPVQYPAGILT
                     EHKWTRESAGLFDVSHMGQAFLLGPDHATVARALEALCPADFLTLKPGMQRYSQLLGE
                     DGGILDDLMVTRSADPDEDGVLMLVVNAGCKEADFAHIAARLPANVRLQRADHRALVA
                     LQGPKAAEVLARHCPDVAGMAFMAALSTRFAGFPCHVSRSGYTGEDGFEMSVHARHVA
                     SLWDALAADPAVRPIGLGARDSLRLEAGLCLYGHDIDTTTSPVEAGLVWSMQKRRRLE
                     GGFPGADRIRKELAEGPSRLRVG"
     regulatory      complement(947..1085)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     regulatory      complement(1086..1177)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(1237..1857)
                     /locus_tag="PLQ11_02905"
     CDS             complement(1237..1857)
                     /locus_tag="PLQ11_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23881.1"
                     /translation="MKTVWNPCRAIALAGLLALGGCGGIGGFGETSPSSGPSLGSLAL
                     FGNPQNLPPERQPELEIEYTCPSVSVIEGAAAYRAGRGTASSDVSHQASLSDVARECR
                     FAGTQFTLKVGVQGRMLIGAQGKAGSFTVPVKVLVKRGDTVVATRAGRVSVSVPAGEG
                     SVAFTHVEDNIVLPITAGRDPSDEYDVYVGFEAGGAPTASRDRRRR"
     gene            complement(1901..2425)
                     /locus_tag="PLQ11_02910"
     CDS             complement(1901..2425)
                     /locus_tag="PLQ11_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HRK23882.1"
                     /translation="MGLFWAAILLLGPVAQAQGTLKEKFGDWQLRCDKVPDKATGKDG
                     EQCILLQSLVDDQAMIDGQKSEINLVVVVLKATDPKASNARKPILRVIAPLGILLPRG
                     LGLKVDSDVIGATGFVRCLPNGCVAEVELDDGLIDKLKKGKEAVFVIFLPPDDGRGLP
                     LVLDGFEKGFQLLQ"
     gene            2781..3662
                     /gene="coxB"
                     /locus_tag="PLQ11_02915"
     CDS             2781..3662
                     /gene="coxB"
                     /locus_tag="PLQ11_02915"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011475186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HRK23883.1"
                     /translation="MNRAIFRLLAAGAALSGLSGVAAALETGRPQPWQMGLQAPATPV
                     AENIAWFHNSLLLPIITVISLFVLMLLVIIVTRFNERKNPTPSKTTHHTMLEVAWTVV
                     PVLILVIIAIPSFRLLKEQIVIPKSDITIKVTGKQWYWTFEYAKDSGGVGFDSIMLSD
                     EDIAKAVKNGAKKDDLPRLLAVDNEAYVPVNKVVRVQVTAADVIHAFAIQSFGVKIDA
                     VPGRLNETWFKATKEGVYYGQCQELCGKDHAFMPVVIRVVSQEKYDAWLKTQKQARLT
                     DAPQFAQTEAAPAAVIR"
     gene            3692..5308
                     /gene="ctaD"
                     /locus_tag="PLQ11_02920"
     CDS             3692..5308
                     /gene="ctaD"
                     /locus_tag="PLQ11_02920"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HRK23884.1"
                     /translation="MATAAAHGDAHHDHPHGWRRFMFSTNHKDIGTLYFILAIAGGIV
                     GGILSIAMRMELQEPGLQYFSNPHTFNVFVTGHGLIMVFFMAMPALIGGFGNWFVPLM
                     IGAPDMAFPRMNNISFWLTPAALLLLVISMFVEGPPGSNGVGGGWTVYPPLSTSGQPG
                     PAMDFAILSLHLAGAASILGAINFIVTIFNMRAPGMTLHRMPLFVWSILVTAFLLVLS
                     LPVLAGAITMLLTDRNFGTTFFAPEGGGDPILFQHLFWFFGHPEVYVIILPGFGLISQ
                     IISTFSRKPVFGYLGMAYAMVAIGFVGFIVWAHHMYTTGISLGAQQYFVAATMVIAVP
                     TGVKIFSWIATMWGGSLRFTTPMVWAIGFVFLFTVGGVTGVVLANAGADRVLHDTYYV
                     VAHFHYVLSLGAVFAIFAGWYYWFPKISGYVIPEWIGKLHFAIAFVGANTLFFPQHFL
                     GLAGMPRRYVDYPDAFAGWNYVSSIGSYIFAAGMLVFFFGVAYAFVRKEKAGNNPWGE
                     GANTLEWTLSSPPPFHQFNTLPRITGSDHH"
     gene            5366..6304
                     /locus_tag="PLQ11_02925"
     CDS             5366..6304
                     /locus_tag="PLQ11_02925"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HRK23885.1"
                     /translation="MSDLGMAGSHQAALLSEAAPSDFIALLKPRVMSLVVFTALVGLV
                     AAPGTIHPFMAAVAILAIAVGGGASGALNMWYDADIDRVMGRTASRPIPAGKVTESEA
                     LSFGLILSAFSVATLGLATNWFAAGLLAFTIFFYAVIYTMGLKRRTAQNIVIGGAAGA
                     FPPMIGWAAVTGGASIESFILFLIIFIWTPPHFWALALVKSDDYARAGVPMLPVVAGP
                     DVTRVQIVLYTLLLVPTGLAPWLTGMAGTLYAAVAILGGAGMLVCAAAIYLRRDGKPA
                     MRACWQMFGFSILYLFGLFAALLVEKLALRVFGWGL"
     gene            6304..6471
                     /locus_tag="PLQ11_02930"
     CDS             6304..6471
                     /locus_tag="PLQ11_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006935704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23886.1"
                     /translation="MAGNAQDVTPPLTEAQMKARRKRSIALALVIGGLCVLFYLITVF
                     KMGAAIMNRQL"
     gene            6479..7084
                     /locus_tag="PLQ11_02935"
     CDS             6479..7084
                     /locus_tag="PLQ11_02935"
                     /inference="COORDINATES: protein motif:HMM:NF003465.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HRK23887.1"
                     /translation="MTGQPTAGSDIAARNRRTGWIVAGAVFGMLGLSFAAVPLYDLFC
                     RTTGYGGRPMVAKEPARSLGTRLYTVRFDAGIAGGLNWRFEAAQASIEVRPGQVTTAH
                     YTVRNVGPTESVGIASFNVLPEITGGYFNKLECFCYTEQALKPGEAREETVVFFLDPA
                     IETDPGLSHVHTVTLSYTFHPAKGAGKSLAANQSSNGEIVQ"
     gene            7127..7999
                     /locus_tag="PLQ11_02940"
     CDS             7127..7999
                     /locus_tag="PLQ11_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HRK23888.1"
                     /translation="MADTHAKTHDYHLVNPSPWPLVGSLSAFIMAVGAVAWMKAMPIA
                     GLKIGPLLFGAGFIGVLFTMYSWWSDVVAEANTGDHTRVVQLHHRYGMILFIASEVMF
                     FVAWFWAYFDVAFYAGEAIQFQRTELTGGHWPPKGIALFNPWELPLFNTLILLTSGTT
                     VTWAHHALLEGDREGLKKGLWATIALGALFTCVQAYEYSHAAFSFKGHIYGATFFMAT
                     GFHGMHVLIGTIFLAVCLIRVYKGHFTPKQHLGFEFAAWYWHFVDVVWLFLFAAIYVW
                     GSNFGKAVMAAAGH"
     gene            8184..8561
                     /locus_tag="PLQ11_02945"
     CDS             8184..8561
                     /locus_tag="PLQ11_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006935707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HRK23889.1"
                     /translation="MDNRYEASGVDPATAAMGGRCPRCGEGRLFNGFVSLNEQCGQCG
                     MSFKFADSGDGPAVFVILLAGFVIIAAMFWVEVTYEPPIWLHLAVFLPLTLIVCLGML
                     RPLKGLMIGLQYRNKAEQGRLDV"
     gene            8561..9289
                     /locus_tag="PLQ11_02950"
     CDS             8561..9289
                     /locus_tag="PLQ11_02950"
                     /inference="COORDINATES: protein motif:HMM:NF014193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HRK23890.1"
                     /translation="MRSRLLIPALVTVPSLAVLIGLGAWQWQRMGEKHVLIATVQERS
                     IQTPEAFPAADRWASLDLASLAYRPVTVSGTFDHGREAHVFFSLAKPVNGVGGPGYLI
                     VTPLLVEGGGVVLINRGFVPADRKEPASRQPGQAVGPVTLTGLIRLPEARNRFAGEND
                     PAKNVFYLRNPDEIARAKRLDHVAPVLIDQRAPMPEGRLPMPGVTQIDIPNNHFQYAL
                     TWWSLAGCLAAIFALFARAKPDQS"
     gene            9344..10744
                     /gene="thrC"
                     /locus_tag="PLQ11_02955"
     CDS             9344..10744
                     /gene="thrC"
                     /locus_tag="PLQ11_02955"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRK23891.1"
                     /translation="MRYISTRGLAPSLSFADALLAGLARDGGLYVPETWPTLTPAAIA
                     GLAGLPFHEVAVRVLKPFVAGTIPDGVLEASARAAYATFGHPAVTPLTQIGPNDFVLE
                     LFHGPTLAFKDVAMQLVARLMDHVLTERGERATIVCATSGDTGGAAAEAFRGRARTNM
                     VILFPKGRVSDVQRRMMTTPTEANVHPIEIDGTFDDCQAIVKALFNNHAFRDRVKLAG
                     VNSINWGRIVAQVTYYFVAAVALGAPHRRVSFTVPTGNFGDIFAGYVAKRMGLPVDRL
                     VVATNVNDILARTLDSGRYEVRGVAATTSPSMDIQVSSNFERLLFEDGGRDAASVRAL
                     MDSLGQSRAFTVPANTLAAIRTGFDAGQTDEAEVATTMRATQAACGYLLDPHTAVAVH
                     VAQQAKRDPAVPMVILSTAHPAKFPEAVAAITAVHPALPERLSRLITDPERMTPLPAD
                     AQAVERFIEQALDGRG"
     gene            10810..11361
                     /locus_tag="PLQ11_02960"
     CDS             10810..11361
                     /locus_tag="PLQ11_02960"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRK23892.1"
                     /translation="MLVVDDGTVTIRPPELDDFAAWSALRAESREFLAPWEPIWPTDD
                     LTQTAFRNRIRRYGDEIGRDEAYPFFVFDQGGKRLVGGLTLGNIRRGAAQAGTLGYWM
                     GARHARQGLMTRAVRLACKLGFLRLGLRRIEAACLPENVASIRLLEKSGFRQEGRARA
                     YLAIAGVRRDHITFALLDDEMPA"
     gene            11457..14360
                     /locus_tag="PLQ11_02965"
     CDS             11457..14360
                     /locus_tag="PLQ11_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRK23893.1"
                     /translation="MLRWSDAGLWRACARVLMAWLCLCMLAVPGGALEIVRISPNIDA
                     IDLLPALETYKSEDDRLTVQTAPGVDGIVRRIEVQASEAGERPNWIVFALKNDSDDTI
                     EKLLVAPRFRLIDSGLIWPDLGSPRIVAVTASMGARPEREDGGDSDVFQLTLEPGATV
                     TFIAELRTPRLPQLSLWDPEAYKDKVNSLTLYRGIVVGIAALLAMFLTVVVAVRGSII
                     FPAAAALAWAVLVYLGIDFGLFAKFLAVRPEAERLYRAGAETVLAGTLVVFLFAYLNL
                     NRWHVRYSVLTSIWILFLMALLALAIFNAPVAAGVARISIGVVALVGFILIVYLSFHG
                     YDRAVMLVPTWFLLLVWCAAVGLVIGGQLRSEFAPPALLGGLVLIVMLIGFTVMQNAF
                     SIGIIARGAVTDAERKALALTGANDYIFDWDVLEDHIFVSPEFEQHLALTRGTLDGPA
                     AAWVDIIHPTDRDRYRATLEAVLEQRRGRINQDFRLRSQDGHSVWYRLKARPVVDNNG
                     EVVRVVGTLTDVTEARMSEERLLRDAVHDNLTGLPNRKLFLERLDAALIFSRADTNLR
                     PTVIGLDLDRFKQVNDSLGPTAADTILLTIARRLSRLMKPADLIGRLAGDSFGVILVS
                     EQDRERILALADLMRRAISAVMIYDGREIIVTATLGVVFPDAAQNSDRDALLKCAELA
                     VTHGKRSGGDRVEVFRAAMQSDRVDRLAVGTELRRALERNELRVLFQPIVRLDDRTIA
                     GFEALVRWDHPRLGRLSPVDFIPVAEETGLIHDLGLFVMERTARELNLWQQALVVEPP
                     IFASVNISSRQLLRHDLLQDVKGIFLRHPVLRGSLKLELTESLIMENPEYAAQMMLRL
                     REIGAGLALDDFGTGYSSLAYLQRFTFDTLKIDQSFVRHTGSGSRPVILRSIIALAHD
                     LKMQVVAEGAETEADTVELSQLGCEYAQGFAFGQPMTVEQARRLIGVSTSEPL"
     gene            complement(14482..15096)
                     /locus_tag="PLQ11_02970"
     CDS             complement(14482..15096)
                     /locus_tag="PLQ11_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HRK23894.1"
                     /translation="MITDRSNGRREPRGFLDGQMLVAMPGMQDERFVRAVIYICAHSS
                     DGAMGLVVNKRSPHLDLPELLTQLKLIDEAAVIRLPDKVGRIEVMQGGPVEKSRGFVL
                     HSTDYFIDHASLPVDDGIAMTVTVDILKAIAAGEGPENAVVALGYAGWSPGQLEQEMQ
                     ENGWLHCPPDPALIFGHDHETKYERALRRIGIDPRMLSSEAGHA"
     gene            15219..16067
                     /locus_tag="PLQ11_02975"
     CDS             15219..16067
                     /locus_tag="PLQ11_02975"
                     /inference="COORDINATES: protein motif:HMM:NF022853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HRK23895.1"
                     /translation="MRSLLCLIVTCLVLGPAAAAETYRASSWVRTAGAEARLILSQAG
                     VSSGPLWGGIEIRLAPGAKTYWRSPGETGVAPVADFAGSRGVKPPVLAFPLPVAFDDG
                     AGGTAIGYTSDLILPFRIEPLSGAGRISVAVKLEFGICQKSLCMPAEAMLRLAPGEGA
                     DEAGLAVRMAETLAMVPVPRALGSTERPAIVTADARQQGTDLMLTITARTLPGDSAPS
                     LFVEGDDVYAVRLESVVNGEARFSARTRREATASGKPLSLLMTLGSNNSAIETAVTLD
                     VGAVRP"
     gene            16231..16716
                     /locus_tag="PLQ11_02980"
     CDS             16231..16716
                     /locus_tag="PLQ11_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRK23896.1"
                     /translation="MTIKVGDRLPDVTFAVMTDEGPQPKSTGDLFNGKKSVLFGVPGA
                     FTPTCHKNHLPGYLTHLDALKAKGVDMVAVVAVNDPFVMKAWAEATGGTGKITFLSDG
                     SAVFAQAAGLALDLTARGLGIRNQRFSMIVEDGVVKALNIEEAPGTVDKTGADTLLKQ
                     L"
     gene            complement(16874..17335)
                     /gene="rnhA"
                     /locus_tag="PLQ11_02985"
     CDS             complement(16874..17335)
                     /gene="rnhA"
                     /locus_tag="PLQ11_02985"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016921596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HRK23897.1"
                     /translation="MTRKVMIWTDGACSGNPGPGGWGALLRYGAVEKELSGGEAETTN
                     NRMELTAAIMALETLTRPCSVDLYTDSQYLRSGITGWIAGWKRNGWKTADKKPVKNID
                     LWQRLDAATTRHSIAWHWVKGHAGDENNERVDLLARAGMAPFKPAPAGQQE"
     gene            complement(17332..18297)
                     /gene="thrB"
                     /locus_tag="PLQ11_02990"
     CDS             complement(17332..18297)
                     /gene="thrB"
                     /locus_tag="PLQ11_02990"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HRK23898.1"
                     /translation="MAVYTDVSVEDLVAFLEPYEVGGLLSFRGIAEGVENSNYVVEAE
                     RGRFILTLYEKRVNEHDLPFFLGLMEHLSGRGINCPLPVRARDGKMLHRLAGRPAALI
                     TFLQGVSYRRPSPTHCGELGRALANLHIAGADFRRRRSNALGLAGWRPLYTLARDGAE
                     RVQHGLRGLIDRELDHLEAVWPTGLPAGVIHADLFPNNVFFMGDRLTGVIDFYFACDD
                     AFAYDLAICINAWCFEADLSFNLTKCRALIAAYDAVRPLNEAEKAALPTLCRGSALRF
                     LLTRLYDYLNVPPGALVRPHDPKEYSKRLRFHQTVASPADYGLVV"
     gene            18461..19297
                     /locus_tag="PLQ11_02995"
     CDS             18461..19297
                     /locus_tag="PLQ11_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRK23899.1"
                     /translation="MQRDLIRQLGTLGATQVRFLFGFPFGLLLLLLALVATGEAVPAF
                     SAAYLAWTLAGGATQIAATALMLKAMTARSFVVTTAYTKTEPVQVAIFGLIVLGDMLT
                     PLLALAILIATAGVMLLAWPAGGRDSGAEGSDRLPALLGLLAGAMFALSAISFRGAIH
                     ALDSGSVWLRASTTLAVGLGLQTCGLLLYMMLRDRATLRAIVAAWRPSLVAGLMGAVA
                     SQFWFLGFALTTAARVRTLALIEVLFAHAVSGRIFDEKVSRREIVGLALVVLGVGLAL
                     NA"
     gene            complement(19287..20252)
                     /gene="ispH"
                     /locus_tag="PLQ11_03000"
                     /gene_synonym="lytB"
     CDS             complement(19287..20252)
                     /gene="ispH"
                     /locus_tag="PLQ11_03000"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HRK23900.1"
                     /translation="MTKPPLDILLCAPRGFCAGVVRAIDAVEEALRRFGRPVYVRHEI
                     VHNRYVVEDLKRKGAIFVDELDEVPDTDAPVIFSAHGVPKSVPAEAGRRHLMAIDATC
                     PLVTKVHREAEIHHRRGRLVILIGHSGHPEVVGTMGQLPDGAVVLVETLEDVARLAPP
                     AGIPLAYVSQTTLSVDDTRGIIEALQARFPAIHAPHKEDICYATTNRQEAVKRVAPQV
                     DALIVVGSPNSSNSQRLREVAERAGCRSAHLLLRAADIDWSVFGAIRSLGITAGASAP
                     EVLVEEMIDAFAARYQVNVRTVSTADESVFFPLPRQLREAPSVTR"
     gene            20374..>20711
                     /locus_tag="PLQ11_03005"
     CDS             20374..>20711
                     /locus_tag="PLQ11_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRK23901.1"
                     /translation="MTPPPARLNARLTTGSTLRHVVTMTFAGSLGLIAIFLVDFLSLF
                     YISLLKDEKLTAGVGYATSVLFVAISFSIGLMIAVSAVVARRLGAGDREAARKAATTG
                     VVLSAATGAAV"
BASE COUNT         3558 a         6597 c         6592 g         3964 t
ORIGIN      
        1 gccgacgcgc aggcgggacg gcccctcggc cagctccttc cggatacggt ccgcgccggg
       61 aaagccgccc tcaagcctgc gccgcttctg catcgaccag accaacccgg cctcgaccgg
      121 cgaggtcgtg gtgtcgatgt cgtggccgta gaggcagagc ccggcctcca gccggagcga
      181 atcgcgggcg ccaagcccga tcggcctgac cgccggatct gcggccagcg cgtcccaaag
      241 actggccaca tgacgggcat gaacggacat ctcgaacccg tcctcgccgg tgtagcccga
      301 tcgcgacaca tggcagggaa atccggcaaa ccgggtcgag agcgccgcca tgaaagccat
      361 gccggccaca tccgggcaat ggcgggcgag cacctctgcc gccttcggcc cctgcaacgc
      421 caccagcgcc cggtgatcgg cccgctgcag gcgtacattg gccggcaggc gggccgcgat
      481 atgggcaaag tcggcctcct tgcaaccggc gttgaccacc agcatcagga cgccgtcctc
      541 atccggatcg gccgaacggg tcaccatcag atcatcaaga atgccgccat cctcgccaag
      601 caactgcgaa tagcgctgca tgccaggctt gagggtgaga aaatcggccg ggcacagggc
      661 ctcaagcgcc ctggcaaccg tggcgtgatc ggggccgagc aggaacgcct gccccatatg
      721 cgagacatca aacaggccgg cgctctcgcg cgtccatttg tgctcggtga ggatgccggc
      781 aggatattga accggcatgt cgtagccggc aaagggcacc atgcgcgcac cggcggcgac
      841 atgcgcatcg taaagtggcg tgcgcaaggg catttgaggc gaatcagcac cggccatcgc
      901 aggctccgtt caacaggggt cgacatccgg atcgaaaccg atccggcatg ccccctctgt
      961 cccgtgacct gagagattca tgcttgcccg gcgaggcgcg cattttctcc gtcggtgagc
     1021 cctgctacct gcctcgcttc caacaaacgc ggcgaagcct gttcaaaggc tgctttccag
     1081 agcggctgcc ccctgcggtc cttttgcctg agcgtttccg gggcggttgc gccttcggcg
     1141 ccggcacaac aatgccggac tctcccgctg ggtttcattt gccgtaagcg cagctaacga
     1201 aggcaaccgc ctcgtgtcaa cctcgcgttc ggttattcac cgccgccgcc ggtcacggct
     1261 ggctgtcggc gcgcctccgg cctcgaagcc gacatagaca tcgtattcat ccgatggatc
     1321 acgccctgcg gtgatcggaa gcacgatgtt gtcctcgaca tgggtgaagg ccacgctgcc
     1381 ctccccggcc ggcaccgaca ccgagacacg tccggcgcgg gtcgccacaa ccgtatcgcc
     1441 ccgcttgacc agcaccttga ccggcaccgt aaagctgccg gccttgccct gggcgccgat
     1501 cagcatgcgg ccctggacgc cgaccttcaa cgtgaattgc gtgccggcaa aacggcattc
     1561 ccgcgccacg tcggacaagg aggcctgatg ggagacatcg gacgaggccg tgccgcgccc
     1621 ggcccgatag gccgcggcac cttcgatgac cgacacactc gggcaagtgt attcaatctc
     1681 cagttccggc tgacgctccg gcggcaggtt ctgcggattg ccgaacaggg cgagcgagcc
     1741 gaggctcggc cccgatgacg ggctggtctc accaaagccg ccaatcccgc cgcagccgcc
     1801 cagcgccaga aggccggcaa gcgcaatggc ccggcacggg ttccaaactg tcttcatcac
     1861 cgcccccttc aacgcaaaac cccggctccg gacagagcac tcactgcaac aactggaagc
     1921 ccttttcaaa accgtcaagc acaagcggca gaccacgacc gtcatcgggc ggcaggaaga
     1981 tgacgaaaac cgcctccttg cccttcttca gcttgtcgat caggccatcg tccagttcga
     2041 cttcggcgac gcagccattg ggcaggcaac gaacaaagcc ggtggcgccg atgacatccg
     2101 aatcaacctt gagaccaagg ccgcgcggca gcaggattcc caacggagcg atcacccgca
     2161 gaatcggttt gcgggcatta ctggccttgg gatcggtggc cttgagcacc accacgacaa
     2221 gattgatctc gcttttctgg ccatcgatca ttgcctgatc gtcgaccagg ctctgcagga
     2281 gaatgcattg ctcgccgtcc ttgccggtgg ccttgtccgg caccttgtcg cagcgcaatt
     2341 gccaatcgcc gaacttctcc ttcagcgtgc cctgcgcctg cgccacaggg ccaagcagca
     2401 gaattgcagc ccaaaagagg cccatgcgca aaaatgccgc agggcgggag agcaaagagc
     2461 gcagcgggaa aaaagcgttc acgggatcgt cctgtcaaaa accgccagcc atcgccttgc
     2521 cggtaacaga ggcggcgcca gtgtcaagga aactggccga tgggttggtc acttcgacgc
     2581 agcttgccac aagttcaatg cgccgatcag ttgcatcaag gccaatgatg tgtttcagag
     2641 gaaagctgac ggggagtgcg ggtcaatcgg cgcacccgtt ttgctgctga ccgaaggctc
     2701 cggatgatct tcgtggcggc ggcatgtggg gatcaggatt gcaggagcgg acagaactca
     2761 tgttcggcac aggaaaaatg atgaatcggg caatttttcg gctgttggcc gcaggggccg
     2821 cgctgtcggg actgagcggg gttgccgccg ccctggaaac ggggcgtccg cagccctggc
     2881 agatggggct tcaggcgcct gctacgccgg tggcggagaa tatcgcctgg ttccacaatt
     2941 cgctgctgct gccgatcatc acggtgatct cgctgttcgt gctcatgttg ctggtgatca
     3001 ttgtcacccg cttcaacgag cgcaagaatc cgacgccgtc caagaccacg catcacacca
     3061 tgctggaagt ggcgtggacc gtcgttccgg tgctgatcct ggtcatcatc gccatcccct
     3121 ccttccgcct tttgaaggag cagatcgtga tcccgaagtc ggacatcacc atcaaggtga
     3181 ccggcaagca atggtactgg accttcgaat acgccaagga ttcgggcggc gtcggtttcg
     3241 actcgatcat gctgagcgac gaggatatcg ccaaggcggt caagaatggc gccaagaagg
     3301 acgatcttcc ccgcctgctg gctgtcgaca acgaggccta tgtgccggtc aacaaggttg
     3361 ttcgcgtgca ggtgacagcc gccgacgtca tccacgcctt cgccatccag tcgttcggcg
     3421 tgaagatcga cgcagtgccc ggccgtctca acgagacctg gttcaaggcg accaaggaag
     3481 gcgtctatta cggccagtgc caggaactgt gcggcaagga ccatgccttc atgccggtgg
     3541 tcatccgcgt cgtttctcag gaaaaatatg acgcctggct caagacccag aagcaggcgc
     3601 gcctgaccga tgcgccgcag ttcgcccaga ccgaggcggc acccgccgcc gtcatccgct
     3661 aattcatacg cataaagacg gggtaactct catggccacc gctgcagctc acggcgacgc
     3721 ccaccacgat catccgcatg gatggcggcg tttcatgttt tccaccaacc acaaggacat
     3781 cggcacgctg tatttcatac tggcgatcgc cggcggcatc gtgggtggca tcctgtccat
     3841 cgccatgcgc atggaactgc aggagccggg tctgcaatac ttttccaatc cgcacacgtt
     3901 caacgtgttc gtgaccggtc acggcctgat catggtgttc ttcatggcca tgccggcgct
     3961 gatcggcggg ttcggcaact ggttcgtgcc gctgatgatc ggcgcgccgg acatggcctt
     4021 tccgcgcatg aacaacatct cgttctggct gacgccggcg gcgctgttgc tgctggtgat
     4081 ctcgatgttc gtcgagggcc cgcccggatc caacggcgtc ggcggcggct ggaccgtcta
     4141 tccgccgctg tcgacctcgg gccagccagg gccggcgatg gattttgcca tcctgtcgct
     4201 gcatctggcc ggtgcggcct cgattctcgg cgcgatcaac ttcatcgtga cgatcttcaa
     4261 catgcgcgcg ccgggcatga cgctgcaccg catgccgctg tttgtctggt cgatcctggt
     4321 caccgccttc ctgctggtgc tgtcgctgcc ggttctggcg ggcgccatca ccatgctgct
     4381 caccgaccgc aacttcggca cgacgttctt tgcgcctgag ggcggcggcg atccgatcct
     4441 gttccagcat ctgttctggt tcttcggaca tccggaagtg tatgtgatca ttctgccagg
     4501 cttcggtctg atcagccaga tcatctcgac cttctcgcgc aagccggtgt tcggctatct
     4561 cggcatggct tacgcgatgg tcgccatcgg tttcgtcggc ttcatcgtct gggcgcacca
     4621 tatgtatacg accggcatca gcctcggcgc gcagcagtat ttcgttgccg cgacgatggt
     4681 gatcgcggtg ccgaccggcg tgaagatctt ctcctggatc gcgacgatgt ggggtggatc
     4741 gctgcgcttc accacgccga tggtctgggc catcggcttt gtgttcctgt tcaccgtcgg
     4801 cggcgtgacc ggcgtggtcc tcgccaatgc cggcgctgac cgcgtgctgc acgacaccta
     4861 ttacgtggtg gcgcatttcc actacgtgct gtcgctcggc gccgtgttcg cgatcttcgc
     4921 cggctggtat tactggtttc cgaagatttc gggctacgtc atcccggaat ggatcggcaa
     4981 gctgcacttt gccatcgcct ttgtcggcgc caacacgctg ttcttcccgc agcacttcct
     5041 cgggctggcc ggcatgccgc gccgctatgt cgattacccg gatgcgttcg cgggctggaa
     5101 ctacgtgtcg tcgatcggct cgtatatctt cgccgccggc atgctggtgt tcttcttcgg
     5161 cgtcgcctac gccttcgtcc gcaaggagaa ggccggcaac aatccgtggg gagaaggcgc
     5221 gaacacgctg gagtggacgc tgtcctcgcc gccgccattc caccagttca acaccctgcc
     5281 gcgtatcacc ggcagcgacc atcactgaga gcttcggggc cggcgttcct cgccggctcc
     5341 gatcaagccg cattgagacc tttgcatgag tgacctgggg atggccggct cgcatcaggc
     5401 ggcgcttctg tcggaagcgg ctccgtccga tttcatcgcg ctgttgaaac cgcgggtcat
     5461 gtcgctcgtg gtgttcacgg cgctggtcgg tctggtcgcg gcgccgggaa ccatccatcc
     5521 gttcatggcg gcggtggcca ttctggccat cgccgtgggg ggcggcgcct ctggcgctct
     5581 caacatgtgg tatgatgcgg atatcgaccg ggtcatgggg cgaaccgcca gccgcccgat
     5641 tcccgccggc aaggtgacgg aaagcgaagc gctgagcttc gggctgatcc tttccgcctt
     5701 ttcggtggcg acgctggggc ttgccaccaa ctggtttgcc gccgggcttc tggccttcac
     5761 gattttcttt tatgcggtga tctacacgat gggcctcaag cgccgcaccg cacagaacat
     5821 cgtgatcggc ggcgcagcgg gcgctttccc gcctatgatc ggctgggctg cggtcaccgg
     5881 cggcgcctcg atcgagagct tcatcctgtt cctgatcatc ttcatctgga cgccgccaca
     5941 tttctgggcg ctggcactgg tgaaatccga cgattatgcg cgcgctggcg tgccgatgct
     6001 gccggtggtg gccggacccg acgtgacgcg ggtgcagatc gtgctttata cgttgctgct
     6061 ggtgccgacc gggctggcgc cgtggctgac cggcatggcc ggcacgcttt atgccgcggt
     6121 ggcgatcctg ggcggtgccg gcatgctggt ctgcgcagcg gcgatctacc tcaggcgcga
     6181 cggcaagccg gcgatgcgcg cctgctggca gatgttcggc ttttccatcc tctacctgtt
     6241 cggcctgttc gcggcgctgc tggtcgagaa gctggcgctt cgcgttttcg ggtgggggct
     6301 gtgatggccg gcaatgcaca ggacgtgacc ccgccgctga ccgaggcgca gatgaaggct
     6361 cggcgcaagc ggtcgatcgc gctggcgctg gtgatcggcg ggctgtgcgt gttgttctac
     6421 ctgatcaccg tgttcaagat gggcgcggcg atcatgaacc ggcagctgtg aggcaaagat
     6481 gacggggcag ccgacggcag gatccgacat cgcggcgcgc aaccggcgca ccggctggat
     6541 tgtggccggc gcggtgttcg gcatgctggg cctgtcgttc gcggcggttc cgctctacga
     6601 cctgttctgc cggaccaccg gctatggcgg ccggccgatg gtggccaagg agccggcgcg
     6661 cagccttggc accaggcttt acacggtgcg tttcgatgcc gggattgccg gcgggctcaa
     6721 ctggcggttc gaggcagcgc aggccagcat cgaggtgcgc cccggacagg tgacgaccgc
     6781 ccattacacg gtccgcaatg tcgggccgac cgaaagcgtc ggcattgcca gcttcaatgt
     6841 cctgccggaa atcaccggcg gctatttcaa caagctcgaa tgcttctgct acaccgaaca
     6901 ggccctcaag ccgggcgagg cgcgcgagga gaccgtggtg ttcttcctcg acccggccat
     6961 cgagaccgat ccgggcctgt cgcacgtcca tacggtgacg ttgtcctaca cattccatcc
     7021 ggccaagggc gcggggaaat cgcttgccgc caaccagtcg agcaacgggg aaatcgttca
     7081 atgacgggga gcgcggtcac aggggtctat actgaagggg agtgcgatgg ctgacaccca
     7141 cgcgaaaacg cacgattatc atctggtcaa tccaagcccg tggccgctgg tcgggtcgtt
     7201 atcggcattc atcatggccg tcggcgcggt cgcctggatg aaggcgatgc cgattgccgg
     7261 gctcaagatt ggcccgctct tgttcggcgc cggcttcatc ggcgttctgt tcacgatgta
     7321 tagctggtgg tccgatgtgg tggccgaggc caataccggc gaccacaccc gggttgtgca
     7381 gctgcatcat cgctatggca tgatcctgtt catcgcttcc gaagtgatgt tcttcgttgc
     7441 ctggttctgg gcctatttcg acgtcgcctt ctatgccggc gaggcgatcc agttccagcg
     7501 caccgagctc accggcggtc actggccacc caagggtatc gcgctgttca atccgtggga
     7561 actgccgctg ttcaacacgc tgatcctgct gacctccggc accaccgtca cctgggcgca
     7621 tcatgcgctg ctggaaggcg accgggaagg gctcaagaag gggctttggg caaccatcgc
     7681 gctcggggcg ctgttcacct gcgtgcaggc ctatgaatac agccatgcag ctttcagctt
     7741 caaaggccac atctatggcg cgaccttctt catggcgacc gggttccacg gcatgcatgt
     7801 gctgatcggc accatctttc tcgccgtctg cctgatccgg gtctacaagg gccatttcac
     7861 ccccaagcag catctcggct tcgaatttgc cgcctggtac tggcacttcg tcgacgtggt
     7921 gtggctgttc ctgttcgccg ccatctatgt gtgggggtcg aatttcggca aggcagtcat
     7981 ggcggcggcc ggccactgat accaaaagtc atttgaaatg actctttggt gcaactcaaa
     8041 aatttcttat cccgttgata aaaatgagaa atttttgaaa cgaaccaagt cgcggcgagt
     8101 tcctcgcctt gcggcttggt atgaggccaa tctctcctgt cgagggcggc ggcgacgtcg
     8161 cccttttcgt ttgcggggcc ggaatggaca atcgttatga agccagcggg gttgatcccg
     8221 ccactgccgc catgggaggc cgctgcccgc gctgcgggga gggccggctg ttcaacggct
     8281 tcgtgtcgct gaacgagcag tgcggccaat gcgggatgtc gttcaagttc gccgattcag
     8341 gcgatgggcc ggcggtcttc gtcatcctgc tggcaggctt tgtgatcatc gctgcgatgt
     8401 tctgggtcga ggttacgtac gagccgccga tctggctgca tctcgccgtg ttcctgccgc
     8461 tgaccctgat cgtctgcctc ggcatgctgc gaccgctcaa gggcctgatg atcggcctgc
     8521 aatacaggaa caaggccgag cagggccggc tggacgtctg atgcgctccc ggctgctcat
     8581 tcctgccctt gttaccgtgc ccagccttgc ggtgctgatc ggcctgggcg cctggcaatg
     8641 gcagcggatg ggggaaaagc atgtgctgat tgcgaccgtc caggagcgca gcatacagac
     8701 gccggaggct ttcccggcgg ctgaccgttg ggcctcgctc gatcttgcca gcctcgccta
     8761 ccggccggtg acggtgagcg gaaccttcga tcacggacgc gaggcgcatg tgttcttttc
     8821 gctggccaag ccggtcaacg gtgtcggcgg gccgggctat ctgatcgtca cgccattgct
     8881 ggttgagggc ggcggagtgg tgctgatcaa ccgcggcttc gtgccggccg accgcaagga
     8941 gccggcaagc cgtcagcccg gccaggctgt cgggccggtg acgctgaccg gcctgatccg
     9001 cctgcccgag gcgcggaacc gctttgccgg cgaaaacgat ccagccaaga acgtgttcta
     9061 cctgcgcaac cccgatgaaa tcgcccgcgc caagcggctg gaccatgttg caccggtgct
     9121 gatcgaccag cgggcgccga tgccggaagg ccggctgccg atgccggggg tgacgcagat
     9181 cgacattccc aacaaccatt tccagtatgc attgacctgg tggtctctgg ccggttgtct
     9241 ggccgccatt ttcgctctgt ttgcgcgggc aaagccggat caatcctgat tttttggcgc
     9301 gacgctctag gattccgggc ccatccggtg ctatcggacc ggcatgcgct acatctcgac
     9361 ccgcggcctt gcgccgtccc tgagctttgc cgatgccctt ctcgccggac tggcacgtga
     9421 tggcggcctt tatgtgcccg agacctggcc taccctgacg ccggccgcca ttgccgggct
     9481 ggcaggcctg ccgttccacg aggtggcggt gcgggtgctc aaacccttcg tcgccggaac
     9541 gattcctgat ggcgtgctgg aggcctcggc gcgcgcggcc tatgccacgt tcggccatcc
     9601 tgcggtaacg ccgctgaccc agatcggccc caacgacttc gtgctggaac tgtttcacgg
     9661 gccgaccctc gccttcaagg atgtggcgat gcagcttgtc gcccggctga tggaccatgt
     9721 cctgaccgaa cgcggggagc gcgccaccat cgtctgcgcc acctccggcg ataccggcgg
     9781 ggcggcggcg gaagcgtttc gtggccgggc ccgcaccaac atggtgatcc tgttcccgaa
     9841 gggccgggtc tccgacgtgc agcggcggat gatgacgacg ccgaccgagg cgaatgtcca
     9901 tcccatcgag atcgacggca cattcgatga ctgccaggcc atcgtcaagg cgctgttcaa
     9961 caaccatgcc ttccgtgatc gggtgaagct tgccggggtc aattcgatca actgggggcg
    10021 cattgttgcg caggtgacct attacttcgt cgcggcggtg gcgctggggg caccgcatcg
    10081 acgggtgagc ttcacggtgc cgacaggcaa tttcggcgat attttcgccg gctatgtcgc
    10141 caaacgcatg ggcctgccgg tggacaggct ggtggtggcg accaacgtca acgacattct
    10201 cgcccgaacg ctcgacagcg gccgctacga agtgcgcggc gtggccgcaa ccacctcacc
    10261 ctcaatggac atccaggtct cgtcgaactt cgaacggttg ctgttcgagg acggcggacg
    10321 cgatgccgca tcggtccgcg ccctgatgga cagcctcggc caatccaggg ccttcacggt
    10381 gccggcaaat acactcgcgg ccatccgcac aggcttcgat gccggccaga cggatgaggc
    10441 agaggtcgcg acgacgatgc gcgctaccca agcggcctgc ggttatcttc tcgatcccca
    10501 tacggccgtt gccgtgcatg tggcccagca ggcaaagcgc gatccggccg tgccgatggt
    10561 catcctgtcc acagcccatc cggccaagtt ccccgaggcg gtggcggcga tcacggcagt
    10621 gcaccctgcc ctgcccgagc gtctttcccg gctgatcacc gatccggagc ggatgacgcc
    10681 gctgccggcc gacgcgcaag ccgtggaacg gttcatcgaa caggcgctgg acggacgcgg
    10741 atgaacatcc gcgaccggct ggcacggctt aaactgatcg gcatccagcc gggcggaccg
    10801 cgttggccca tgctggtggt cgatgatgga accgtgacca ttcgcccgcc ggaactcgat
    10861 gatttcgccg cctggtccgc cctcagggcc gaaagccgcg aatttctggc gccctgggag
    10921 ccgatctggc cgacggatga tctgacccag accgcatttc gcaatcgtat ccgtcgttat
    10981 ggcgatgaaa tcggccgcga cgaggcctat ccgttcttcg ttttcgacca ggggggcaag
    11041 cgtctggtcg gcgggctgac gctgggcaac atccggcgcg gcgcggccca ggccggcacg
    11101 ctcggctatt ggatgggtgc tcgccatgcc aggcaggggc tgatgacacg ggcggtgcgg
    11161 cttgcctgca agctgggctt cctgcggctt ggcctcaggc gcatcgaggc tgcctgcctg
    11221 ccggaaaatg tcgcatcgat ccggctgctg gaaaaatccg gcttccggca ggaagggcgc
    11281 gcgcgcgcct atctggcgat cgccggtgtt cggcgcgacc atatcacctt cgcgctgctc
    11341 gacgacgaga tgccggcatg attctggcac tccgctcgga cgagagcgca aaaaacggtg
    11401 cgacaaagcc cctccggctt gccgccatgc agcagcaggg ctaccaaggg gccatgatgc
    11461 ttcgatggtc cgatgccggc ctgtggcgcg cctgcgcgcg cgtcctgatg gcgtggctct
    11521 gtctttgcat gctggccgtt ccgggcggtg cactcgagat tgtccgcatc agtccgaaca
    11581 ttgatgccat cgatctgctg ccggcgctcg agacctacaa atccgaggat gatcggctga
    11641 cggttcagac cgcgcccgga gttgacggca ttgtccgccg catcgaggtg caggccagcg
    11701 aggctggaga gcgccccaac tggatcgtct ttgcgctgaa gaatgattcg gatgacacga
    11761 tcgaaaagct tttggtggcg ccgaggttcc ggctgatcga ttccggtctg atctggcccg
    11821 atctcggctc gccccgcatc gttgccgtga ccgccagcat gggcgcgcga ccggaacgcg
    11881 aggacggcgg cgattccgac gtgttccagc tcacgctgga acccggcgca acggtcacct
    11941 tcatcgccga attgcgcacg ccacgcctgc cccagctctc gctgtgggat cccgaagcct
    12001 acaaggacaa ggtcaacagc ctgacgctct accggggaat cgtggtcggc atcgcggcgc
    12061 tgctggcgat gttcctgacg gtggttgttg cggtgcgcgg ctcgatcatc tttccggcgg
    12121 cggcggcgct cgcctgggcg gtgctggtct atctgggcat cgatttcggc ctgttcgcca
    12181 aattcctcgc cgttcggccg gaagcggaac gcctctatcg cgccggcgcc gagaccgtgc
    12241 ttgccggaac gctggtggtg ttcctgttcg cctatctcaa tctcaaccgc tggcatgtcc
    12301 gctattcagt gctgaccagc atctggatcc tgtttctgat ggcactgctg gcgctggcca
    12361 tcttcaatgc tccggtggcg gccggcgttg cgcgcatctc gatcggtgtc gtggcgctcg
    12421 tcgggttcat cctgatcgtc tacctgtctt tccatggcta tgatcgggcc gtgatgctgg
    12481 tgccgacctg gttcctgctg ctggtctggt gcgcggcagt ggggctggtc atcggcggtc
    12541 agctcaggag cgagttcgcc cccccggccc tgttgggcgg tctggtgctg atcgtgatgc
    12601 tgatcggctt tacggtgatg cagaacgcgt tctcgatcgg gatcattgcc cgcggcgccg
    12661 tcaccgacgc cgagcgcaag gccctcgccc tgaccggagc caatgattac atcttcgact
    12721 gggatgtgct tgaggaccat attttcgtca gtcctgaatt cgagcagcat ctggccctca
    12781 ctcgcggcac gctggatggc cccgctgcgg cgtgggtcga catcatccac ccgaccgaca
    12841 gggaccgtta cagagcgacg ctggaggccg tgctcgaaca gcgccgcggc cggataaacc
    12901 aggatttccg cctgcgcagc caggatggcc acagcgtctg gtatcggctg aaggcccggc
    12961 cggtggtgga caacaatggc gaggtcgtgc gcgtggtcgg cacgctcacc gatgtcaccg
    13021 aagcgcgcat gtcagaggaa cggctgctgc gcgatgccgt gcatgacaac ctcaccggcc
    13081 tgcccaaccg caagctgttt ctcgaacgac tcgatgcggc gctgatcttc tcgcgcgccg
    13141 atacgaacct tcgtccgact gtgatcggac tcgatcttga ccgcttcaag caggtcaacg
    13201 actcgctcgg cccgacggcg gcagacacga ttctcctgac cattgcccgg cgcctgtcac
    13261 gactgatgaa gccggcggac ctgatcggcc ggctggccgg cgacagtttc ggggtcattc
    13321 tcgtctcgga gcaggatcgc gagcggattc tggcacttgc ggacctgatg cgccgggcga
    13381 tcagcgcggt gatgatctat gacgggcgcg agattatcgt caccgcaacg ctgggcgtcg
    13441 tgttcccgga cgctgcgcag aacagcgacc gcgacgccct tttgaaatgt gccgaactcg
    13501 ccgtcaccca tggcaagcga tcgggcggtg accgggtgga ggtattccgt gccgcgatgc
    13561 aatcggatcg ggtggatcgg ctggcggtgg gaaccgaact gcggcgggcg ctcgaacgca
    13621 acgaattgcg cgtgctgttc cagccgatcg tgcggctcga cgaccgcacc atcgccggct
    13681 tcgaggcgct ggtccgctgg gaccatccaa ggctgggccg cctgagcccg gtcgatttca
    13741 tcccggtggc cgaggaaacc gggctgatcc atgatcttgg cctgttcgtg atggaacgga
    13801 cagcccgcga actcaacctg tggcagcagg cgctggtagt ggagccgccg atcttcgcct
    13861 cggtcaatat ttcgagccgg caattgctgc gtcacgatct gttgcaggat gtcaagggca
    13921 tcttcctgcg ccatccggtg ttgcgcggat cactgaagct tgaattgacc gaaagcctga
    13981 tcatggaaaa ccccgaatat gccgcgcaga tgatgctgcg gctgcgggag atcggcgccg
    14041 gactggcgct cgatgatttc ggcaccggct attcctcgct tgcctatctg caacggttca
    14101 ccttcgatac gctcaagatc gaccagtcgt tcgtccgcca taccggaagc gggtcccgac
    14161 cggtcattct gcgctccatc atcgcgctgg cgcatgatct gaagatgcag gttgtggcgg
    14221 aaggcgcaga gaccgaagcc gatacggtgg agctgtccca actcggctgc gaatatgccc
    14281 agggttttgc cttcggccag ccgatgacgg tcgaacaggc gcggcggttg atcggcgtca
    14341 gcaccagcga gccactgtaa ttcagccctg ccgagcgaag ttcaacctaa ccgcacaaga
    14401 ggcccgtcgg tcggcccggc ccatccgtct gctggctgcc atgctcctga gccgcgcagc
    14461 atcaaacagg aaatcatcgt ttcaggcgtg ccccgcctcc gacgacagca tgcgcgggtc
    14521 gatgccgatg cggcgcaagg cccgttcata tttggtctcg tgatcgtggc cgaagatcag
    14581 cgccggatca ggcgggcaat gcagccagcc attctcctgc atttcctgtt caagctggcc
    14641 tggcgaccag ccggcataac cgagcgccac aacggcattc tccggtccct cacccgccgc
    14701 gatcgctttg agaatatcga ccgtcacggt catggcgatg ccgtcatcaa ccggcagcga
    14761 ggcgtgatca atgaaatagt cggtcgagtg cagcacgaag ccacggcttt tttcgaccgg
    14821 cccgccctgc atcacctcga tgcggccgac cttgtccgga agccggatca ccgccgcctc
    14881 gtcgatcagc ttgagctggg tcagcagttc gggaaggtca agatgtggcg aacgcttgtt
    14941 gacgaccagc cccatcgccc cgtctgacga atgggcgcag atatagatga cagcacggac
    15001 aaaccgctca tcctgcatgc cgggcatggc aacgagcatc tgtccatcga gaaagccgcg
    15061 cggttcgcgc cggccatttg agcgatcggt gatcattcgc caatgctaat gggcttgcgg
    15121 caaatgccaa gcggggactt taacttttat cggtgaatgt tcagggcaag gcgctccaat
    15181 gccccgcttc cgccgtcttg tccgatcaca ggtcgcccat gcgctcgctg ctgtgtctta
    15241 tcgttacctg ccttgtgctc ggtccggcgg ctgcagccga gacctatcgc gcgtcgtcct
    15301 gggtgcggac agcaggcgcc gaggcgcggt tgatcctgtc ccaagccggc gtttcatccg
    15361 gcccgctgtg gggcgggatc gaaatccggc tggcgccggg cgccaagacc tactggcgca
    15421 gtcccggaga aaccggcgtt gcccccgtcg ctgattttgc cggttcgagg ggggtcaagc
    15481 cacccgttct ggcctttccc ctgccggtcg ccttcgatga cggggcaggc ggaacagcca
    15541 tcggctatac cagtgatctg atcctgccgt tcaggatcga accgctgagc ggggccggac
    15601 gcatcagcgt tgccgtgaaa ctcgagttcg gaatctgcca gaagagccta tgcatgccgg
    15661 ccgaggcaat gctgcgtctg gcgcccggcg aaggggccga cgaggcggga ctagccgtcc
    15721 gcatggccga gacgctggcg atggtcccgg tgccccgtgc gcttggttcg acggaaagac
    15781 cggcgattgt cacggcagac gcgcgccagc agggcacaga tctgatgctg acgatcaccg
    15841 ccaggaccct ccccggtgac agcgcaccgt ccctgtttgt cgaaggcgac gatgtctatg
    15901 ctgtccggct tgagtctgtc gtaaacggcg aagcgcgttt ttccgcacgg acacggcgcg
    15961 aagccaccgc ttccggaaag cccctgtcgc ttttgatgac gcttggcagc aacaatagcg
    16021 ccatcgagac cgccgtgacc ctcgacgttg gcgcggttcg cccctagatc acactgtgac
    16081 tgatggactc atccaaacac agataacgtg atcgctatca atagtttaga acatgcttgt
    16141 cgcgattaac cggcttccac ttagtcgcag catgctctaa gtaaggagcg gggcctgccg
    16201 atcatggcgc aggccgaggg agcttttccg atgacgatca aggttggaga ccgcctgccg
    16261 gacgtgacat tcgcggtgat gaccgacgag gggccgcagc ccaagagcac aggcgatctg
    16321 ttcaatggca agaagtccgt cctgttcggc gtgccgggtg ctttcacgcc tacctgccac
    16381 aagaatcacc tgccgggcta tctcacccat cttgatgcac tcaaggccaa gggcgtcgac
    16441 atggtggcag tcgtggcggt caatgacccg ttcgtgatga aggcttgggc ggaagcgacc
    16501 gggggaaccg gcaagatcac cttcctgtcg gacggcagcg ccgtgtttgc ccaggcggcg
    16561 ggactggcgc tggatctgac ggcccgcggc ctcggcatcc gcaaccagcg tttttcgatg
    16621 atcgtcgagg atggcgtggt caaggcgctc aatatcgagg aagcacccgg cactgtcgac
    16681 aagaccggcg ccgatacttt gctgaagcag ctctgatacc aagtcgcaag gcgaggaact
    16741 cgccgtgact tggttcgttt caaaaatttc tcacttttat caacgagatg agaaattttt
    16801 gagttgctcc aaagagtcat tttcaatgac ttttgggatg agcagcagcg agtggccggg
    16861 atcaaaaatc ccgtcactcc tgctgtccgg cgggcgccgg cttgaacggt gccatgccgg
    16921 cgcgggccag aagatcgacc cgctcgttgt tctcatcacc cgcatgaccc ttgacccagt
    16981 gccaggcgat cgaatggcgc gtggtggcgg cgtcgagccg ctgccagaga tcgatgttct
    17041 tgaccggctt cttgtcagcg gttttccagc cgttgcgctt ccatccggca atccagccgg
    17101 tgatgccgga gcgcagatac tggctgtcgg tgtaaagatc gacgctgcat gggcgcgtca
    17161 gcgtttccag cgccatgatt gccgccgtca gttccatgcg gttgttggtg gtctcggctt
    17221 cgccgccgct gagttccttt tcgaccgccc catagcgcag cagagcaccc cagccgccgg
    17281 ggccgggatt gcctgaacag gccccatcgg tccagatcat gaccttgcgg gtcacacgac
    17341 cagcccgtag tcagccggtg aggccaccgt ctgatgaaat ctcaggcgct tggaatattc
    17401 cttcggatca tgcggccgca ccagcgcccc gggcggcacg ttgagataat catagagccg
    17461 cgtcagcagg aaccgcagcg cgctgccgcg gcacagggtc ggcagcgcgg ccttttctgc
    17521 ctcattcagc gggcgcaccg catcataggc ggcgatgagt gcgcggcatt tggtcaggtt
    17581 gaacgacaga tcagcctcga agcaccaggc attgatgcag atcgccagat cgtaggcgaa
    17641 ggcatcgtcg caggcgaaat agaagtcgat cacaccggtc agccgatccc ccatgaagaa
    17701 tacattgttg gggaataggt cagcatgaat gacgccggcg ggcaggccgg tgggccacac
    17761 tgcttcaaga tgatcgagtt cccggtcgat gaggccgcgc aggccatgct gaacgcgctc
    17821 ggcgccgtcg cgggcgagcg tgtagagcgg gcgccagccg gccagcccca gagcattgga
    17881 ccggcgacga cgaaaatcgg caccggcaat gtgcagattg gccagagcgc gccccagttc
    17941 cccgcaatgg gtcggactgg ggcgacgata ggaaacgccc tgcagaaaag tgatcagcgc
    18001 cgccggccgt ccagccagcc ggtgcagcat cttgccgtca cgggcgcgca ccggcagcgg
    18061 gcagttgatg ccgcgaccgc tcagatgttc catcaggccg aggaaaaacg gcagatcgtg
    18121 ctcgttcacc cgcttttcgt agagcgtgag aatgaagcgg ccacgctcgg cctcgacaac
    18181 gtagttgctg ttctcgacac cttcggcaat gccgcgaaac gacagcagcc ccccgacctc
    18241 gtagggttcg agaaaggcga caagatcttc gacagagacg tcggtgtaga cagccatagg
    18301 acgcacgaaa aagggatgaa aagcctgttg ggcagcgagc gaccgtgtta cggctcgagg
    18361 cggcaaaggt caaatcccgc ccgggtcaag ggagcaggtt ttcgtgttgt gggcgctctt
    18421 tacgatcctg gcagcgacgg cgcagaccgc ccgcaatgcc atgcagcgcg acctcatccg
    18481 tcaactcggc acattgggcg cgacacaggt gcgcttcctg ttcggctttc cgttcggtct
    18541 cctgctgctg ttgctggcgc ttgtggccac cggcgaggcg gtgccggcct tttcggcagc
    18601 ctatctggcc tggacactgg ccggcggggc aacccagatt gcggcgaccg cgctgatgct
    18661 gaaggcgatg acggcgcgat ccttcgttgt gacgaccgcc tataccaaga ccgagccggt
    18721 gcaggtggca atcttcggcc tgatcgtgct cggcgacatg ctgacgccgc tgctggcgct
    18781 cgcgatactg attgcaacgg ccggcgtgat gctgctcgcc tggcccgccg gagggcgcga
    18841 cagcggcgcg gagggctccg atcggctccc ggcgctgctg ggattgctgg ccggggcgat
    18901 gttcgcgctg tcggcgatca gctttcgcgg cgcgatccat gcgctggaca gcggcagcgt
    18961 ctggctgcgc gcctccacga ccctggcggt cggccttgga ttgcagacct gcgggctgct
    19021 cctctacatg atgctgcgcg accgggcgac gctccgtgcc attgttgccg cctggcgtcc
    19081 gtcgctggtt gccgggctga tgggtgcggt tgcctcgcag ttctggttcc tcggatttgc
    19141 cctgaccacg gcggcacgcg tgcgcacgct ggccctcatc gaagtgctgt tcgcccatgc
    19201 ggtctccggg cggatcttcg acgaaaaggt cagccggcgg gagattgtcg ggctggcgct
    19261 ggtggtgctt ggcgtcggcc tggcgctcaa cgcgtgacgg agggcgcttc ccgcagctgc
    19321 cgcggcagcg ggaagaacac gctttcatcg gctgtcgaga ccgtcctgac gttgacctga
    19381 tagcgcgcgg caaacgcgtc gatcatttcc tcgaccagca cctcgggcgc cgaggcgccg
    19441 gccgtgatgc ccagcgaacg gatggcgccg aacaccgacc agtcgatatc ggccgcccgc
    19501 agcagcagat gcgcgctgcg gcagccggca cgttcggcaa cctcgcgcag gcgttgcgag
    19561 ttcgacgaat tgggactgcc gaccacgatc agcgcatcga cctgcggcgc cacgcgcttg
    19621 accgcttcct ggcggttggt ggtggcatag cagatgtctt ccttgtgcgg cgcatggatg
    19681 gccggaaagc gcgcctgcaa ggcctcgatg atgccgcgcg tatcatcgac cgagagcgtt
    19741 gtctggctga cataggcgag cggaattccc gcgggcgggg cgaggcgggc gacatcctcc
    19801 agcgtttcga ccagaaccac cgcgccatcg ggcaattgcc ccatcgtccc caccacttcg
    19861 ggatggcccg aatggccgat gaggatgacc agacggccgc gacggtggtg gatctccgcc
    19921 tcgcgatgaa ccttggtgac cagcggacag gtggcatcaa tcgccatcag atgacgacgc
    19981 cccgcctcgg cgggaacgga tttcggcacg ccatgggcag aaaagatcac cggcgcatcg
    20041 gtatcgggca cttcatcgag ttcgtcgaca aagatcgccc ccttgcgctt taagtcctcg
    20101 accacatagc ggttgtgaac gatctcatgg cgcacataga ccggacggcc aaaccgccgg
    20161 agtgcctctt ccaccgcatc gatggcgcgc acgacaccag cgcagaagcc gcgcggagca
    20221 cacagcagaa tatcgagcgg cggttttgtc atggtcggtc catcatcggt gcgccctact
    20281 tgtcgcctgt cgccgacgcc atcgtcaagt ctgccgcgta tcggcatttt cggtgcttga
    20341 cgcaaccggc ggcgaccgcg acaacccttg gcaatgaccc cgcctcccgc ccgcctcaat
    20401 gcccgcctga cgaccggctc caccctgcgc catgtcgtga ccatgacctt cgccggctca
    20461 ttggggctga tcgcgatctt cctcgtcgat ttcctgtcgc tgttctacat ctcgctgctc
    20521 aaggacgaga agctcaccgc cggtgtcggc tatgccacca gcgtgctgtt cgtcgccatt
    20581 tccttcagca tcgggctgat gatcgccgtc tcggcagtgg tggcacggcg gctcggtgcg
    20641 ggcgaccgcg aggcggcgcg caaggccgca accaccggcg tggtgctgag tgcggcaacc
    20701 ggcgcagcgg t
//
LOCUS       DAOMXG010000024        20368 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_1565_length_20368_cov_10.613794, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000024 DAOMXG010000000
VERSION     DAOMXG010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 20368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20368
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1565_length_20368_cov_10.613794"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /gene="dapB"
                     /locus_tag="PLQ11_03010"
     CDS             <1..711
                     /gene="dapB"
                     /locus_tag="PLQ11_03010"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HRK23902.1"
                     /translation="LERLGSPALGKDAGVLAGLPAQGVPILADMNAALAKADAIIDFT
                     APAATLELLKAAAERKLVHIIGTTGLSVSDHAAIAAAGRSARIVQSGNMSLGVNLLAA
                     LVRRAAAALSEDFDIEVLEMHHRHKVDAPSGTALLLGEAAAEGRQIPLADHSVRVRDG
                     HTGARREGDIGFATLRGGSVIGDHSVIFAGAGERLTLSHHAEDRSLFASGAMRAALWA
                     KDQPPGVYSMADVLDLRR"
     gene            727..1236
                     /locus_tag="PLQ11_03015"
     CDS             727..1236
                     /locus_tag="PLQ11_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004674643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRK23903.1"
                     /translation="MVRRLDASAAASLVDDLAAILAACVAGGAGVGFVLPFSEADAAG
                     WWRSVLPMVVAGERLLFGGFVDGHLAGTVSLVPATPQNQPHRADISKMLVHPSARRRG
                     LGVALMQAAEAEARRLGKTLLTLDTVTGSGAEQLYAGLGWQKSGVIPNYALSPTRSYD
                     STTVMWKAL"
     gene            1233..1844
                     /locus_tag="PLQ11_03020"
     CDS             1233..1844
                     /locus_tag="PLQ11_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HRK23904.1"
                     /translation="MIALQPGLHYHPGYFSRPAQEALLADIRANVRNAPLFTPVMPKT
                     DQPFSVRMSNCGALGWVSDQAGYRYQPHHPQTGLPWPPIPPALADLWQAVSGYPHPPE
                     ACLVNYYAPGARMGLHQDRDEADLAAPVVSVSLGATALFRFGGLRRNDPTRSVRLESG
                     DVLVIGGASRLCFHGIDRLFPGTSMLIADDGRINLTLRRVTRP"
     gene            complement(1875..3224)
                     /locus_tag="PLQ11_03025"
     CDS             complement(1875..3224)
                     /locus_tag="PLQ11_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019995513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HRK23905.1"
                     /translation="MDKAPHDAGAPGWRRAGGEVSLPEAHHTIGVPKTPSIWRKFLAF
                     AGPGYLVAVGYMDPGNWATSLAGGSRFGYALLFVVLMSNIMAIILQSLCARLAFASGR
                     DLAQACRDSYPRPVVAILWFLAEIAIIATDLAEVIGTAIGLKLLFGIPLELGVVITAL
                     DVFLIMALQRIGFRAIEAVVIGLLTLIAACFAAQIAMVSPVWSEVFAGFSPTTQLLTD
                     QDMLYLALGIIGATVMPHNLYLHSAIVQTRAIGETLPERREAMRLATIDSTVALMLAL
                     LINASILILAATAFYKTGRHEVAELGEAHALLAPIVGTTAPVLFAVALIACGLNSTVT
                     ATLAGQIVMEGFIALRLRPWLRRLVTRLVAIVPAAGVTILMGEKETAKLLILSQVILS
                     LQLPFAVIPLVVFTASRAKMGALVAPRWLTAAAALIAAIIVVLNLKLLSDTVSGWLG"
     gene            complement(3305..4108)
                     /locus_tag="PLQ11_03030"
     CDS             complement(3305..4108)
                     /locus_tag="PLQ11_03030"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HRK23906.1"
                     /translation="MTLLTMIQAAILGFVEGLTEFLPVSSTGHILLLGHFLGFDSPTK
                     TFEVLIQLGAILAILSVYFKKLLDIALGLGSDPQKRRFVLAILVAFVPAAIIGGLFSK
                     VIKAYLFNPFVVCTSLIAGGLVLLVVDETEFEKKYDDVMEFPLATALRIGLFQCLAMV
                     PGVSRSGATIVGAMLSGASKRAAAEFSFFLAMPTMAGAFAKDLLDNYKFLAAKDFGLI
                     TIGFVMAFLSGLLVVRGFLDFVSRHGFTPFAYWRIGVGIIGLAGLALFG"
     gene            4262..4954
                     /locus_tag="PLQ11_03035"
     CDS             4262..4954
                     /locus_tag="PLQ11_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017485713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRK23907.1"
                     /translation="MPEFYHHPLCPQSRYIRLVMGELGMEAAQIEERVWERRREFLML
                     NPAGTTPVLVEENVLPIPGADVIAEYLDETRGLVLGERRLLPEDPLSRVEVRRLCDWF
                     NRKFHEEVSHYLVVEKVHKRFLGPEKGGGAPEGAILRVARANVRYHVKYIGHLLGQRN
                     WLAGDRLTYADLAAAAHLSVCDFLGDVPWNEDETAKQWYARLKSRPSFRPLLADRILG
                     MTPAAHYTNLDF"
     gene            4852..6093
                     /gene="queG"
                     /locus_tag="PLQ11_03040"
     CDS             4852..6093
                     /gene="queG"
                     /locus_tag="PLQ11_03040"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015822828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HRK23908.1"
                     /translation="MVRPPEVAALVPPAAGRPDPRNDAGRPLHKSRFLNGHALKAALE
                     SRAKALGFDAFGVAAVASLPDRSRSLADWLAAGHHASMSWMAERHSERSSPTRLWPDV
                     RSVLMVGVNYGNIPDPLAALERRDSATLALYAQRRDYHAVIKGRLKDLAGFLVSRAGG
                     DVKVFVDTAPVMERPLAMVSGIGWQGKNTMLVSRKFGNWLLLGAVFSTLEIEPDVAEP
                     DHCGSCTRCLDRCPTAAFPAPYVLDARRCIAYLTIEHHGPVPAELRPAIGNRVFGCDD
                     CLAVCPWNKFASAAREAKLATQGMLAALPLSELARLDDPAFRSLFAATPVKRLGRDRF
                     LRNVLIAIGNSQDPALAASARARLEDAAPAVRAMAIWALSRLLPAAAFQALAADRSEQ
                     EAVAEVRAEWDAALAQSRKPR"
     gene            6118..6801
                     /locus_tag="PLQ11_03045"
     CDS             6118..6801
                     /locus_tag="PLQ11_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008506342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HRK23909.1"
                     /translation="MKRSEINAAIAHAEAMLAEHRFILPPFARWTPADWQERRGQCAE
                     IIRAGLGWDITDFGQGDFAGLGLTLFTVRNGDAAALSSGKGMVYAEKVIIVQDGQRCP
                     MHYHFHKTEDIINRGGGILALELVETDAAGKADPSREVVVRTDGIERRLPAHGVLRLA
                     SGESVTLTPGLAHAFWAEGGDVFVGEVSSVNDDATDNAFLEPVGRFPTIEEDARPTRL
                     IVGDTRLLA"
     gene            complement(6849..7763)
                     /locus_tag="PLQ11_03050"
     CDS             complement(6849..7763)
                     /locus_tag="PLQ11_03050"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HRK23910.1"
                     /translation="MTLTDSSQDYRLVRSAIGFIAHSFRDQPEVETIAEAVGLTPADL
                     NRLFRRWAGLTPKAFLQAVTLDHARRLLKDSASVLDATFDSGLSGPGRLHDLFVTHEA
                     MSPGEYKSGGAGLTLRFGFHPSPFGLALVIAHERGLCGLAFADAEAPEGAGATPGDPA
                     ASKSSAPAIGRLAALADMRQRWPRAAFVEDAGFVAPYARRIFQADLWRGDQPLRVFMI
                     GSDFEVRVWESLLRIPMGCAATYSDIARHIGKPQAARAVGAAVGRNPISFVVPCHRVL
                     GKTGALTGYHWGLTRKQAMLGWEAGRAG"
     gene            complement(7816..8322)
                     /locus_tag="PLQ11_03055"
     CDS             complement(7816..8322)
                     /locus_tag="PLQ11_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HRK23911.1"
                     /translation="MRQASDSATGPVAPAETVLFSATLFPHRSLSPGGGRLIVALVAL
                     VAGAASLPFLLAGAWPVGGFLGLDVALLYWCFSRNHRQARAYEEVLLTRLELFVRRVS
                     WRGKVSEWRFNPVWVRLQAEEDPEYGMQRLAIVQRREEVEIGACLAPFERADFAHSFG
                     QALARARR"
     gene            8357..9046
                     /gene="nth"
                     /locus_tag="PLQ11_03060"
     CDS             8357..9046
                     /gene="nth"
                     /locus_tag="PLQ11_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HRK23912.1"
                     /translation="MAKASTRQNTRRSARPPAALGPAEVEAVFAAFRAANPQPRGELD
                     YVNPFTLLVAVVLSAQATDAGVNKATRELFRLADTPEKMLALGEDRVRDLIRTIGLFR
                     TKAKNVIALSGKLIAEFDGVVPASREALVSLPGVGRKTANVVLNIAFGQPTIAVDTHL
                     FRVSNRLPLAPGRTPEAVEAGLERIVPEAFRLHCHHWLILHGRYICKAIKPTCGRCPV
                     RSWCRFPGKTA"
     gene            complement(9188..9730)
                     /gene="grpE"
                     /locus_tag="PLQ11_03065"
     CDS             complement(9188..9730)
                     /gene="grpE"
                     /locus_tag="PLQ11_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010684633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HRK23913.1"
                     /translation="MPLPEAPVDPVAALEAEKLDLKDRLLRSLADMENLRRRTERDVA
                     DARSYGIASFARDMLTVADNLARALASLPAASREAGDDMLRAFIEGVELTERDLLKTL
                     DKHGVKKREPLGEKFNPNFDQAMFEVPDPAQPNGTVMQVVQPGYVIGERVLRPSMVGV
                     TRGGPKASDRPAEAGEPTQH"
     gene            complement(9842..12649)
                     /locus_tag="PLQ11_03070"
     CDS             complement(9842..12649)
                     /locus_tag="PLQ11_03070"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HRK23914.1"
                     /translation="MRQTPAPGRRTPAFLPTDGTSLWPADAGLLFEGALDAIAVGASP
                     EEMRAEIVRWGKSVLATARDQAHSVLLTQGGMACARALSLAQDAIIRALFQFVTERLY
                     PVANPSSGERIAVCAVGGYGRETLAPGSDVDILFLLPVETSWGESIAEAMLYPLWDLG
                     LKVGHATRSVDECIKTALGDMTVRTALLEARLIAGDAALFATFESRFEKEVVAGSAAD
                     FVQAKVVEREQRLTRAGSSRYLVEPNIKEGKGGLRDLQMLFWIARYVYRVKQRGDLVA
                     AGLFTQRELDLFNRCEEFLWRVRCHMHFATKRAEERLSFELQRVLAERLRYRARGPLS
                     PVERFMKHYFLVAKDVGDLTAIVSAALESQEKRPTALFDRFIGKFRRRHSLSGTKDFL
                     VDKGRITVADDDIFARDPVNLLRLFWYADRHQLPVHPDATRLVTLSLGRTDARLREDG
                     EANRLFLEILVTRHGNPENVLRRMHATGLLGRFIPDFGRINAMMQFSMYHHYTVDEHT
                     LRAIGILSDLENGKLKEALPVAHEVLPGIVNRRVLYVALFLHDIGKGREEAHEIVGAR
                     IARDLCPRLGMSAAETETVAWLVEHHLDMSQTAQSRDISDPRTITTFTAAVQTIERLK
                     LLLVLTVCDIRAVGPQVWNGWKGQLLRALYWEAEAVLAGGHTASERDGRVLAAKAELR
                     KALLDWDDSAFDAYAARHNVAYWTKVDLDRQVRHARLLAKADRTSERFQTEITTSKFN
                     GVTEVTVLALDHPRLLSIITGACAAAGANIAEAQIFTTSDGLALDSIFLSRAFEDDAD
                     ELRRAQRVASHIVKALKGEIRLPDVVASRAGRARPNTTFSVTPNVVIDNQLSAKATVI
                     EVTGLDRPGLLYELTTEIGKLNLNIISARIITYGEKAVDVFYVTDLTGGKVEQPTRRA
                     TITRHLIDVLEPPALAPARQT"
     gene            complement(12646..13605)
                     /gene="gshB"
                     /locus_tag="PLQ11_03075"
     CDS             complement(12646..13605)
                     /gene="gshB"
                     /locus_tag="PLQ11_03075"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019906381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HRK23915.1"
                     /translation="MHRSTLLVAVQMDPIDRINIKGDSTFALMLEAQARGHRLVYYTT
                     DTLTMRDAQVFASVAPVTVRDREGDHFTLGPAERIDLSTTDVVLMRQDPPFDMNYITA
                     THLLERIHPGTLVVNNPAHVRNAPEKLFVTEFPDLMPATLITRNRAEIEAFRAEHGDI
                     VMKPLYGNGGAAVFKVAARDANFGSLYDLFSVTFREPWVCQRFLPEVKDGDKRIILVD
                     GEAAGAVNRVPAADDIRSNMVRGGAARPTDLTPREQEICARIGPALRERGLIFVGIDV
                     IDGNLTEINVTSPTGLRAIKRLGGPDLAVSIWDAIEARLGRRA"
     gene            complement(13710..14069)
                     /locus_tag="PLQ11_03080"
     CDS             complement(13710..14069)
                     /locus_tag="PLQ11_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012605552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HRK23916.1"
                     /translation="MTRHHRAYGFGLDAEWLAELLLRAKGYRILARRFAAAGGEVDLV
                     ARRGGTLIFVEVKARAGLEQALEAITPLKRQRISRAARAYLGRLEALPAIIRMDAVFV
                     SPHALPRHLENFFELDL"
     gene            complement(14066..14962)
                     /gene="rsmI"
                     /locus_tag="PLQ11_03085"
     CDS             complement(14066..14962)
                     /gene="rsmI"
                     /locus_tag="PLQ11_03085"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HRK23917.1"
                     /translation="MTSTYTLLGLQAETTRCPPGLYIVATPIGNLKDITLRALETLAA
                     ADAILAEDKRVTKVLLAHYGISTPILSYHEHNAELMRPDILARLAGGAALALVSDAGT
                     PLVSDPGYKLVQAAVEAGHAVTALPGASAVLAALVLAGLPSDRFFFEGFLPPRSAERR
                     RRARQIAGIDATIVLFEAPHRLDESLADLAAILGNRPAAVARELTKKFETIRRGTLDE
                     LAAHYAHSEKPRGEIVILIAPASGGESAPGDDVVDARLRSALAEHSPRDAVALVAADL
                     GLPRRAVYARALLLQGRNGDGQ"
     gene            15231..16418
                     /locus_tag="PLQ11_03090"
     CDS             15231..16418
                     /locus_tag="PLQ11_03090"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HRK23918.1"
                     /translation="MRRRIRKWTGAFGVAALLAACADSSSVFPPLNQPSTPSAGTSQP
                     ISGNTIGSGPVRVALILPLTAANGAVPAQSLRNAAEMAIAEFNSQDMTIMVKDDRGTP
                     EGAQAAAQEALAQGAEAIMGPLFAPTVQSVASVARAAQRPVISFSTDTSVAARGVYLL
                     SFLPQSDVRRIVSYAASRGKKSFAALIPQSAYGNVAEVEFTQAVAAAGGSVVAVERYQ
                     PTKASLDAAVAKLKGSMGRADTLFLPDDGNGIPVVAAALRTAGIGGPSVQLIGTGLWN
                     EERVLKTPSMSGGWFAAPAGTGFQSFAQRYRARFNSDPTRIATLSYDATALLAALART
                     QGTRRFTEQVLTNPSGFSGQDGVFRFRADGLNERGLTIYQVNNGAAMVLQPAPSSFGQ
                     AGG"
     gene            complement(16599..17762)
                     /gene="hemW"
                     /locus_tag="PLQ11_03095"
     CDS             complement(16599..17762)
                     /gene="hemW"
                     /locus_tag="PLQ11_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HRK23919.1"
                     /translation="MIAAPPFDAGFAVYVHWPFCAAKCPYCDFNSHVRRDPVDEPRFI
                     AAFRREIATWAERTPGRLVSSIFFGGGTPSLMQPATVGAILDAIGAAWPVAGNVEVTL
                     EANPTSVEAGRFRGYRAAGVNRVSLGVQSLNPVDLAFLGRLHSVEDALAAIAIARGTF
                     ERTSFDLIYARPGQTISGWQTELRRALDLGPTHLSLYQLTIEPDTPFDRLASDGKLVV
                     PDTEAARALYDATQALCADHGLPAYEISNHARPGEESRHNLVYWRYGEYAGLGAGAHG
                     RAVTKEGRIAIANEKSPERWLSLVEAKGHGGVEADILTDEQQADEFLLMGLRLREGID
                     PARFARLRGRTLDDRRVADLVEHRMVEVTESGRLRVTAEGFPVLDAVVADLAA"
     gene            complement(17759..18379)
                     /gene="rdgB"
                     /locus_tag="PLQ11_03100"
     CDS             complement(17759..18379)
                     /gene="rdgB"
                     /locus_tag="PLQ11_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018045121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HRK23920.1"
                     /translation="MAAAPSLRRLGGRVVIATHNAGKLVEMIDLLAPHGVDVVSAGQL
                     GLAEPAETGTMFSENAGVKARAAAAATGIVAIADDSGICVDALDGAPGIFTADWAGEP
                     RDYGRGMARIAAELSRRGLTPTGQQAHFVSSVVVAWPDGSEAVFEGRVFGTLAFPPRG
                     SNGFGYDPIFVADGESRTFGELLPEEKKRHSHRSRAFAAMIAALVT"
     gene            complement(18382..19095)
                     /gene="rph"
                     /locus_tag="PLQ11_03105"
     CDS             complement(18382..19095)
                     /gene="rph"
                     /locus_tag="PLQ11_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012452405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HRK23921.1"
                     /translation="MRPSKRAPDELRKVTMERGVARYAEGSCLIKFGETHVLCTASLE
                     ERGPPWLRGSGKGWVTAEYAMLPRATLERTRREVTAGKPSGRTQEIQRLIGRSMRAVV
                     DLAAIGERQIVLDCDVIQADGGTRTASITGAWVALNDCLAWMRARDLVKTNPLKDHVA
                     AVSCGIFRGVPVLDLDYAEDSNAETDANFVMTGSGGIVEVQGTAEGAPFSQSELLSLL
                     SLAQGGIRRLVDLQKMAVA"
     gene            19213..20307
                     /gene="hrcA"
                     /locus_tag="PLQ11_03110"
     CDS             19213..20307
                     /gene="hrcA"
                     /locus_tag="PLQ11_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HRK23922.1"
                     /translation="MTKDRPINPATPLPEAFDARARDIFRHIVDGYLANGEPVGSRHI
                     SRQLQMPLSPASVRNVMQDLEDLGLIYAPHTSAGRLPTELGLRLFVDAMLEIGDVSAE
                     DKRQIEAQVRAAGAGAPDKFEDVLAEASRLVSGLARGAGVVIASKAGKRWKHIEFVRL
                     DPSRALLVLVGEDGQLENRLLPLPAGLPASALTQAANYLNARLGGRSLGEVRAEIETR
                     RQEMEREIDALTARLIDSGLASLSEGADGATQLIVRGQANLLEDVRAAEDLDRIRRLF
                     ADLESKKDLIDLLTRAESADGVRIFIGSENKLFSLSGSSLIAAPFRDSQQRIVGVLGV
                     IGPTRLNYARIVPMIDYTAQVIGRMLDRRR"
BASE COUNT         3762 a         6599 c         6459 g         3548 t
ORIGIN      
        1 ttggagcggc tgggttctcc ggcgctcggc aaggatgccg gcgtcctggc gggcttgccg
       61 gcgcagggcg taccgattct ggccgacatg aatgcggctc ttgccaaggc ggatgccatc
      121 atcgatttca cggctccggc tgcaacgctg gagttgctca aggccgctgc tgaacgcaag
      181 ctcgtccata tcatcggcac gacaggcctg tcggtctccg accatgcggc gattgccgca
      241 gccggtcgct cggcgcgcat cgtccagtcc ggcaacatga gccttggcgt caatctgctg
      301 gcggcactgg tacgacgggc ggcagcggcg ctcagcgagg acttcgatat cgaggttctc
      361 gaaatgcacc atcgccataa ggttgatgcg ccgtccggga cagcgctgct gctcggcgaa
      421 gcagcggcgg aaggccggca gatcccgctc gccgaccatt cggtgagggt ccgcgacggt
      481 cacaccggcg cgcggcggga aggcgacatc ggttttgcta ccctgcgggg cgggtcggtc
      541 atcggcgacc attcggtgat ctttgccggt gcgggcgagc gactgaccct cagccaccat
      601 gccgaggatc gctcgctgtt tgcctcgggc gccatgcgtg cagccctgtg ggccaaggat
      661 cagccgccgg gcgtctattc gatggccgat gtgctcgacc tccggaggtg atggatggaa
      721 gggtgcatgg ttcgccggct ggacgcgtca gccgcggcca gccttgttga cgatctagct
      781 gccatcctgg ctgcctgtgt agccggagga gccggtgtcg gatttgtcct gccgtttagc
      841 gaggccgatg ctgccggctg gtggcgctcc gtcctgccga tggttgttgc cggagagcgt
      901 ttgctgttcg gcggttttgt tgacggacac cttgccggca ccgtatcgct cgtgccagcc
      961 acaccgcaga accagccgca ccgggcggat atatccaaaa tgctggtgca tccctccgct
     1021 cgccgccgtg gactgggcgt tgcgctgatg caagcggccg aagccgaggc acggcggctg
     1081 ggaaagacgc tgctgaccct cgatacggtg accggcagcg gcgccgagca gctctatgcc
     1141 gggctgggct ggcagaagag cggggtcatc ccgaactatg cgctgtcgcc gacccgcagc
     1201 tacgacagca ccacggtgat gtggaaggcg ctgtgatcgc gttgcagccc ggccttcact
     1261 accatccggg ctacttctcc cgcccggcgc aggaggccct gctggcggac atccgcgcga
     1321 atgtgcgcaa cgcgccgctg ttcacgccag tcatgccgaa aaccgaccag ccattctcgg
     1381 tgcggatgag caattgcggc gctctcggct gggtctcgga ccaggccggc tatcgctatc
     1441 agccgcatca tccgcaaacc ggcctgccgt ggccgccgat cccgcccgca ctggcggatc
     1501 tctggcaggc cgtcagcggc tacccgcatc cgcccgaggc ctgcctggtc aattactacg
     1561 cgccgggcgc gcgcatgggc ctgcatcagg atcgcgacga ggcggatctg gccgccccgg
     1621 ttgtttcggt ctcactggga gcgacggcgc tgtttcgttt cggcgggctg cgccgcaatg
     1681 atccgacccg atcggtccgg cttgaaagcg gcgatgtgct ggtgatcggc ggagcctcgc
     1741 gcctttgttt ccatggcatc gaccggcttt tcccgggcac ttctatgctt attgccgacg
     1801 atggccggat caacctcacc ttgcggcggg taacaagacc ctgataccgg cggcccgcga
     1861 actcggcatg accttcagcc tagccagccc gaaaccgtat cagacagcag cttgaggttc
     1921 aggacaacga tgatcgcggc gatgagcgcc gctgccgccg tcagccagcg gggcgcgaca
     1981 agcgcgccca tcttggcgcg cgaagccgtg aaaaccacca gtggaatgac cgcgaacggc
     2041 aattgcagcg acaggatcac ctgcgacagg atcagcagct tggcggtctc cttctcgccc
     2101 atcagaatgg tgacgcccgc tgccgggacg atcgcgacga gccgcgtgac caagcgcctg
     2161 agccagggcc gcagccgcag ggcgatgaac ccttccatga cgatctgacc ggccaaagtc
     2221 gcggtcaccg ttgagttgag accgcaggcg atcaacgcca ccgcaaacag caccggcgcg
     2281 gtcgtgccca cgatcggtgc cagcagcgcg tgggcctcgc ccagctccgc cacctcgtgg
     2341 cgaccggtct tgtagaaagc ggtggccgca agaatgagga tggaggcgtt gatgagcagg
     2401 gcaagcatga gagcgaccgt cgaatcgatg gtggccagac gcatggcctc gcgtcgctcg
     2461 ggcagcgttt cgccgatcgc ccgcgtctgc acgatcgccg aatgcaggta gagattgtgt
     2521 ggcatgaccg tggcgccgat gataccaagc gccaaataga gcatgtcctg atcggtcagc
     2581 agctgcgtcg tcggcgagaa tccggcaaat acctcgctcc agaccgggga gaccatggca
     2641 atctgcgccg caaaacaggc tgcgatcaaa gtgagcaggc caatcaccac cgcctcgatg
     2701 gcgcgaaagc cgatccgctg cagcgccatg atcagaaaca catcgagcgc cgtgatcacc
     2761 acgccaagct ccagcgggat gccgaacagc agcttgagcc cgatagccgt accgatcacc
     2821 tcggccaggt ccgtcgcgat gatggcgatc tccgccagga accacaaaat ggcgacaaca
     2881 gggcgcggat aagaatcgcg gcaggcctgt gccaggtccc gtcccgaggc aaaggccaga
     2941 cgcgcgcaca aggattgcag aatgatcgcc atgatgttgg acatcagcac gacaaacagc
     3001 aacgcatagc caaatctgga accaccggcg agcgacgtcg cccagttgcc gggatccatg
     3061 tagccgaccg cgaccagata gcctgggccg gcaaaggcga ggaacttgcg ccagatgctc
     3121 ggcgtcttcg gaaccccgat ggtatgatgg gcctcaggca gggaaacctc cccgccagcc
     3181 ctgcgccagc cgggtgcccc tgcatcatgc ggcgctttat ccatggccta cctgcaaatg
     3241 caaatcattc gcaattaaag gtgggccaga ttaccttaaa ttgcaatagc ctgttaaaac
     3301 cgcttcagcc gaacagagcc aggccggcca ggccgatgat cccgaccccg atgcgccaat
     3361 aggcaaacgg ggtgaagcca tgccgcgata cgaaatccag aaagccgcgc acaaccagca
     3421 gcccggacag gaaggccatg acaaagccga tggtgatcag gccgaaatcc ttggcggcaa
     3481 gaaacttgta attgtcgaga aggtccttgg cgaaggctcc cgccatcgtg ggcatggcca
     3541 gaaaaaagga aaactctgcc gcagcccgct tgctcgcccc cgacagcatg gcgccgacaa
     3601 tcgttgctcc cgagcgcgat acgcccggca ccatcgccag gcactggaac aagccaatcc
     3661 gcagcgccgt cgccagcggg aactccatca catcgtcgta tttcttctcg aactctgttt
     3721 cgtccaccac cagcagcacc agaccgccgg cgatcagcga ggtgcagacg acgaagggat
     3781 tgaagagata cgccttgatg accttggaaa acagtccgcc aatgattgcg gccggcacga
     3841 aggcgacgag aattgccagg acaaagcgcc gcttctgcgg gtccgacccg agccccaggg
     3901 caatatcgag cagcttcttg aaatagaccg acagaatggc gagaatggcg ccaagctgga
     3961 tcagcacctc gaaggtcttt gtcggcgagt caaatccgag gaagtgaccc agcagcagga
     4021 tatgcccggt ggacgatacc ggcaggaatt ccgtcaggcc ctcgacaaat ccgagaatgg
     4081 cggcctgaat catcgttaga agggtcatcg ctacctgccg ggctcatgaa gcgccgccag
     4141 gtccagcagc gccgcgacgg gatgtcgcca cgattgagat acatctgatt cgcttctata
     4201 cgcggaaagc gcttagcgtc ccgttagtgg gcccctgtct ccagcctggt tgttcatcgg
     4261 catgcccgag ttctatcatc acccgctctg cccccagtcc cgctacatcc gcctggtcat
     4321 gggcgagctt ggcatggagg cagcccagat cgaagaaagg gtatgggagc gccgacgcga
     4381 attcctcatg ctcaatcctg ccggaaccac cccggttctt gttgaagaga acgtgctgcc
     4441 gatccccggc gccgacgtca tcgccgaata tctcgatgag acccgcggtc tcgtcctggg
     4501 cgaacgcagg ctgctgccgg aggacccgtt gtcacgcgtc gaggtccggc gactctgcga
     4561 ctggttcaat cgcaagttcc atgaggaagt ttcgcattat ttggtggtcg agaaagtgca
     4621 caagcgcttc ctcgggccgg aaaagggcgg tggggctccg gaaggtgcga tcctgcgtgt
     4681 ggctcgcgcc aatgtgcgct atcatgtcaa atacatcggc cacctgctcg gccagcgcaa
     4741 ctggctggcc ggtgaccgtc tgacctatgc cgatctggcg gctgccgccc atttgtcggt
     4801 atgtgatttt ctcggggacg tgccgtggaa cgaggacgag acggcaaagc aatggtacgc
     4861 ccgcctgaag tcgcggccct cgttccgccc gctgctggcc gaccggatcc tcggaatgac
     4921 gccggccgcc cattacacaa atctcgattt ctgaacggcc acgcccttaa ggccgcactg
     4981 gagagccgcg ccaaggcgct cggcttcgat gccttcggag ttgcggcggt ggcatcactg
     5041 ccagaccgga gcaggtccct tgccgactgg ctcgctgccg gacatcacgc cagcatgagc
     5101 tggatggcgg agcggcacag cgagcgctcc tcgccgacac gattgtggcc cgacgtgcgc
     5161 tcggttctga tggtcggggt caattatggc aacatccccg atccgcttgc cgcgcttgag
     5221 cgccgcgaca gcgctacgct ggccctctac gcccagcggc gcgattatca cgctgtgatc
     5281 aaaggtcgcc tgaaggatct ggccggcttt ctggtgtccc gcgccggcgg cgatgtgaag
     5341 gtgtttgtcg ataccgcccc ggtcatggag cggccgctgg cgatggtgtc gggtattggc
     5401 tggcagggca agaacacgat gctggtcagc cgcaaattcg gtaactggct gctgctcggt
     5461 gccgttttct caacgctcga gatcgagccg gacgtggccg agcctgacca ttgcggttcc
     5521 tgcacccgct gcctcgatcg ctgtccgacg gcggcttttc cggccccgta tgtgctggat
     5581 gcgcggcgat gcattgccta tctgacgatt gagcatcatg gcccggtccc agccgaactt
     5641 cggcccgcca tcggcaatcg ggtgttcggc tgcgacgatt gccttgcggt gtgtccatgg
     5701 aacaagtttg cctcggcggc gcgcgaagcc aagctcgcca cacagggcat gctcgccgcc
     5761 ctgccgcttt ctgaactcgc ccggcttgat gatcctgcat ttcggtccct gttcgccgcc
     5821 accccggtca agcgccttgg ccgggatcga ttcctgcgca atgtgctgat tgccatcggc
     5881 aactcgcagg acccggcact tgcggcttcc gctcgcgcgc ggcttgagga tgcggccccc
     5941 gccgttcggg ccatggcaat ctgggcactt tctcggctgt tgccggcggc cgcgtttcag
     6001 gcccttgctg cggacaggtc tgaacaggaa gcggttgccg aggtgcgtgc tgaatgggac
     6061 gccgcactgg cacaatcccg caagccgcgg taacatcccc ccagtcaggc cagaaagatg
     6121 aaacgctccg aaatcaacgc cgccattgcc catgccgagg ccatgctggc cgagcatcgc
     6181 ttcattctgc ccccgtttgc ccgctggacg cccgcagact ggcaggagcg gcgagggcaa
     6241 tgcgccgaga tcatccgtgc cgggctgggc tgggacatca ccgatttcgg ccagggcgac
     6301 tttgccgggc tcggattgac gctgtttacg gtgcgcaatg gcgatgcagc ggcgctcagc
     6361 tccggcaagg gcatggtcta tgcggaaaag gtcatcatcg tgcaggatgg ccagcgctgc
     6421 ccgatgcact accattttca caagaccgaa gacatcatca accgcggtgg gggcatcctg
     6481 gccctcgaac tggtcgagac cgatgcggcc ggcaaggccg atccttcccg cgaggttgtc
     6541 gtgcgcaccg acggcatcga gcggcgactt cccgcccatg gtgtgctgcg cctggcttcc
     6601 ggcgagagcg tcacgctcac ccccggcctt gcccatgcct tctgggcgga gggcggcgat
     6661 gtttttgtcg gcgaggtgtc ctcggtcaat gacgatgcca ccgacaatgc ctttcttgaa
     6721 ccggtcggtc gctttccgac catcgaggag gatgcccggc caacccggct gatcgtcggc
     6781 gatacccggt tgctggcata ggatcatcga acgttcgggt gcctggaacg gtttgttgtc
     6841 atcctgcttt aacccgcccg ccccgcctcc cagccgagca tcgcctgctt gcgcgtcaga
     6901 ccccaatgat agccggtaag agcgccggtt ttcccgagca cacggtggca tggcacgaca
     6961 aaggagatgg ggttgcgccc caccgccgcg cccacggcgc gcgctgcctg cggcttgccg
     7021 atatggcggg cgatgtcgga ataggtagcc gcgcatccca tcggaatgcg caacaggctc
     7081 tcccagaccc gcacttcgaa atcgctgccg atcatgaaca cccggagcgg ctggtcgccg
     7141 cgccacagat ccgcctggaa gatgcgccgg gcatagggag cgacaaaccc ggcatcctcg
     7201 acaaaagcgg cacgcggcca gcgctgccgc atgtctgcaa gcgcggcgag gcggccgatg
     7261 gccggcgccg aactcttgga agcggctgga tcgccgggcg ttgccccggc gccctccggg
     7321 gcttcggcat cggcaaaggc aagaccacac aacccccgtt catgggcgat gaccagcgcc
     7381 agcccgaatg gcgagggatg aaagccgaaa cgcaacgtca accccgcccc gccggacttg
     7441 tattccccgg ggctcatcgc ttcatgggtg acgaacagat catgcaaccg gccggggcct
     7501 gaaagaccgc tgtcaaaggt tgcatcgagc acgctggccg aatccttcag cagtcgccgg
     7561 gcatgatcga gcgttacggc ctgcaggaag gctttcgggg tcaggcccgc ccagcgacgg
     7621 aacaggcggt tgaggtcggc gggtgtcaat cccaccgctt cggcaatcgt ctcgacctcc
     7681 ggctggtcgc ggaaactgtg cgcaatgaac ccaatcgcgg agcggacaag gcggtagtcc
     7741 tgggaggaat cggtcagtgt catgcaggca atcctgccat ccgtctgaca gccttgccac
     7801 ccgattccgt tgcccctacc gacgcgcccg cgccagtgcc tgcccgaagc tgtgcgcgaa
     7861 atcagcgcgc tcgaacggtg ccaggcaagc gccaatctcg acttcctcgc gccgctggac
     7921 aatggccagc cgctgcatgc cgtattcagg gtcctcttcc gcctgcaacc ttacccagac
     7981 aggattgaaa cgccattcgc tgaccttgcc gcgccagctg acccggcgga cgaacagttc
     8041 cagccgcgtc agcagcactt cctcataggc gcgcgcctga cgatgattgc gactgaagca
     8101 ccaatagagc agagccacat cgagcccgag aaatccgcca accggccagg ctccggccag
     8161 caggaacggc agcgatgccg ctccggcaac cagcgccacc agagcaacga tcagccgccc
     8221 cccgcccggg ctgagcgacc tgtgcgggaa cagggtcgcc gaaaacagca ccgtttcggc
     8281 tggcgcaacc ggtccggtcg cgctatcgct tgcctgccgc atggtcatcc cctcgagccc
     8341 atacggatga aagcctatgg caaaagcctc gacgcgtcaa aacacccggc gttctgctcg
     8401 cccgcccgcc gcactcggtc cggcggaggt cgaggcggtg tttgccgcct tccgtgccgc
     8461 caacccgcag ccgagggggg agcttgacta cgtcaacccg ttcaccttgc tggtcgcggt
     8521 ggtgttgagc gcgcaggcga ccgatgccgg ggtgaacaag gcaacgcggg aattgtttcg
     8581 cctcgccgat acccctgaaa agatgctggc attgggcgag gacagagtgc gggacctcat
     8641 cagaaccatc ggcttgttcc ggaccaaggc gaaaaacgtc atcgccctgt cgggcaaact
     8701 gatcgccgag tttgacggcg tggtgccggc cagccgtgag gcgctggtct cgctccccgg
     8761 agttggccgc aagaccgcca atgtcgtgct caacatcgcc ttcggccaac cgaccatcgc
     8821 ggtggataca catctgttcc gcgtgtcgaa ccggttgccg ctggcgccgg gcaggactcc
     8881 ggaggcggtc gaagcggggc ttgaacggat cgtgccggaa gcgttccgtc tccactgcca
     8941 tcattggctg atcctgcatg gccgatacat ctgcaaggcg atcaagccga cttgcgggcg
     9001 ctgtccggtc aggtcctggt gccgcttccc cggcaagaca gcctgagaga gcatagccgc
     9061 cgcctcttgg cacggctggc gagcacacgc caagcctgca cctcaggaag tacttatcac
     9121 tttgatcaac atgagaaatt cttgaaagga acccgcactc ggtgagttcc ttccatgaga
     9181 ttttctatca atgctgggtc ggctcgccgg cctcggcggg cctgtcggag gctttcgggc
     9241 cgccgcgcgt gacccccacc atggacgggc gcagcacgcg ctcgccgatc acatatccgg
     9301 gctggaccac ctgcattacg gtgccgttgg gctgagccgg atcaggcacc tcgaacatcg
     9361 cctgatcgaa gttggggttg aatttctcgc ccagcggctc gcgttttttc accccgtgct
     9421 tgtcgagggt cttcaacagg tcgcgctcgg tcagttcaac gccctcgatg aaggcgcgca
     9481 gcatgtcatc gcccgcctcg cgcgaggcgg caggcaggct cgccagggcc cgggcaaggt
     9541 tgtcggcaac cgtcagcatg tcgcgcgcaa agctggcgat tccgtagctg cgtgcatccg
     9601 caacgtcgcg ctcggtgcgg cggcgcaggt tttccatatc ggccagcgac cgcagcagcc
     9661 ggtccttgag gtcgagcttt tctgcctcca gcgcagcgac aggatcgacc ggcgcctcgg
     9721 gaagcggcac ctcgtcggcc gggttcacag gagattcggg cgagcgttcg ggtttggaca
     9781 cgataaccga ccttcacgat gatgggttgc gcccgtgata tcgcggcgca acggaggaaa
     9841 atcaagtctg ccgcgccggg gccagtgcag gcggctcgag cacatcgatc agatgccgtg
     9901 tgatggtggc gcggcgcgtc ggctgctcga ccttgccgcc ggtcagatcg gtgacataaa
     9961 aaacgtcaac cgctttttcg ccataggtga tgatgcgagc cgagatgatg ttcaggttga
    10021 gcttgccgat ttcagtggtc agttcataaa gcagaccagg ccggtcaagc ccagtcacct
    10081 caattactgt tgccttcgcc gaaagctgat tgtcgatcac gacattggga gtgacggaaa
    10141 aagtcgtgtt cggccgcgcc cgcccggctc gagacgctac cacatccggc agcctgatct
    10201 cgcccttcag cgccttgacg atgtggctgg ccacgcgttg ggcgcgacgc aattcgtcgg
    10261 cgtcatcctc gaaggcgcgc gacaggaaga ttgaatcgag tgccagaccg tcggatgtgg
    10321 tgaaaatctg cgcttcggca atattggcgc ctgccgctgc gcaggcgccg gtaatgatcg
    10381 atagaagccg cgggtgatcg agcgcaagga ccgtcacttc ggtcacgcca ttgaatttgg
    10441 acgtggtaat ttcggtctga aagcgctcgc tcgtccggtc ggccttggca agcagccggg
    10501 catggcggac ctgtcggtcg aggtcgacct tggtccagta agcgacattg tggcgggcgg
    10561 cataggcatc aaatgccgaa tcgtcccagt caagcagggc cttgcgtagc tcggccttgg
    10621 ccgccagcac ccgcccgtcg cgctcggacg cggtgtgtcc ccctgccagc accgcctccg
    10681 cctcccaata gagcgcgcgc aacaactggc ccttccagcc gttccaaacc tgcgggccga
    10741 ccgcacggat atcgcagacg gtcagcacca gcaggagttt caggcgctcg atggtctgaa
    10801 cggctgccgt gaaggtagtg atggtgcgcg gatcggagat atcgcggctt tgcgccgtct
    10861 gcgacatgtc cagatgatgc tcgaccagcc aggccaccgt ttcggtttct gccgcactca
    10921 tgccgagacg cgggcagaga tcgcgcgcga tgcgggcgcc gacgatctca tgggcctctt
    10981 cacgcccctt gccgatgtca tgcaggaaca atgcgacata caggactcgg cggttgacga
    11041 ttccgggcag cacctcgtgg gccaccggca gcgcttcctt cagcttgccg ttctccagat
    11101 cggacaggat gccgatcgcc cgcagcgtgt gctcatccac cgtgtagtgg tgatacatcg
    11161 aaaactgcat catcgcgttg atgcgaccga aatcgggaat gaatcgtccg agcagaccgg
    11221 tcgcatgcat gcgccgcagg acattttcgg gattgccgtg gcgggtgacc agaatctcga
    11281 gaaacaaccg gttggcttcc ccatcctcgc gcaggcgcgc atcggtccgg ccgagcgaca
    11341 gggtgaccag ccgcgtggca tcgggatgga cgggcaactg atggcggtcc gcataccaga
    11401 acagccgcag aagattgacc ggatcgcgcg caaagatgtc gtcgtcggcc accgtgatgc
    11461 ggcccttgtc aacaaggaaa tccttggttc ccgacagcga gtgccgccgc cggaacttgc
    11521 cgataaagcg atcaaacagc gccgttggcc gcttctcctg cgattcgagc gcagcggaga
    11581 cgatcgccgt caggtcgcca acgtccttgg cgacgagaaa gtaatgcttc atgaaccgct
    11641 cgaccgggct gagcgggccg cgcgcacggt agcggagccg ctcggcaagc acgcgctgca
    11701 attcgaaact gagccgctcc tccgcgcgct tggtggcaaa atgcatgtgg cagcgcacgc
    11761 gccacagaaa ctcctcgcag cgattgaaca ggtccagctc gcgctgcgtg aacaggccgg
    11821 ccgccacgag atcgccgcgc tgcttgacgc ggtagacgta gcgggcgatc cagaacagca
    11881 tctgcagatc gcgcaggccg cccttgcctt ccttgatatt cggctcgaca aggtagcgtg
    11941 acgaaccggc acgggtaagc ctctgctcgc gttcgaccac cttggcctgg acgaaatcgg
    12001 cagccgaacc ggcaacaacc tccttctcaa accgggattc aaaggtcgca aacaacgctg
    12061 catcgccggc aatcagccgc gcttccagca aggctgtgcg caccgtcatg tcacccagtg
    12121 cggtcttgat gcattcgtca acgctgcggg tggcatggcc gaccttgaga cccagatccc
    12181 ataacggata gagcatggcc tcggcgatgc tctcgcccca ggacgtttcg accggcagca
    12241 ggaacaggat atcgacatcc gatcccggcg ccagggtctc gcggccatag ccgccgacgg
    12301 cgcagacggc gatccgctcc ccggatgacg gattggcgac cggatagagc cgttcggtca
    12361 caaactggaa cagcgcccgg atgatcgcat cctgcgccag cgacaacgcc cgggcgcagg
    12421 ccatgccacc ctgtgtcagc aacaccgaat gggcctgatc gcgggctgtg gccagcaccg
    12481 acttgcccca gcggacgatc tcggcacgca tttcttcagg gctcgcgccg acagcaatgg
    12541 catccaaagc cccttcgaac agaaggccgg catcggctgg ccatagcgag gtgccatcgg
    12601 ttggcagaaa ggccggtgtt cgccggccgg gagcgggcgt ctgcctcacg cgcgcctccc
    12661 cagccgcgcc tcgatcgcgt cccagatgct gacggcaagg tcggggccgc caagccgctt
    12721 gatggcgcgc aggccggtgg gcgaggtgac gttgatttct gtcagattgc cgtcgatgac
    12781 atcgataccg acaaagatca gcccgcgctc gcgcaaggcc gggccgatcc gggcacagat
    12841 ttcctgctcg cggggtgtca ggtctgtcgg ccgggccgcg ccgccgcgca ccatgttgga
    12901 gcgaatgtca tcggcagccg gaacgcggtt gacagcaccg gcggcttcgc cgtcgaccag
    12961 gatgatccgc ttgtcgccgt ccttgacctc gggcaggaac cgctggcaca cccagggctc
    13021 gcggaaggtg accgagaaca gatcatagag cgagccgaaa ttggcatccc gcgccgccac
    13081 cttgaacacc gccgcaccgc cgttgccgta gagcggcttc atgacgatat cgccatgttc
    13141 ggcccggaac gcttcgattt ctgcccggtt gcgggtgatc agcgtcgccg gcatcagatc
    13201 gggaaactcg gtgacaaaca gcttttccgg cgcattgcgt acatgcgccg gattgttgac
    13261 caccagcgtc ccggggtgga tacgctcgag cagatgggtt gcagtgatat agttcatatc
    13321 gaaaggcgga tcctggcgca tcagcaccac atctgtcgta gacaggtcga tccgttcggc
    13381 tggcccgagg gtgaaatggt cgccttcgcg atcgcgcacg gtcaccggag caaccgacgc
    13441 aaacacctgt gcatcgcgca tggtcagcgt atcggtcgta taatagacca gacgatggcc
    13501 gcgcgcctgc gcctccagca tcagcgcaaa ggtcgaatcg cccttgatgt tgatccggtc
    13561 gatcgggtcc atctgcacgg cgacgagaag cgttgagcga tgcattgcgg gtccatccat
    13621 caggaaacga gcgattctag tgccagttgg cgtaagagca aagcgctgga cgcaaatcca
    13681 cgtcggggcc ttacgatcgg agactccatc tagaggtcca gctcgaaaaa gttctccaga
    13741 tgccgcggca aggcatgggg ggacacgaag acagcatcca tgcggatgat cgcaggcaag
    13801 gcttcgagac gcccgagata ggcgcgtgcc gcccggctga ttcgctggcg cttcagcggt
    13861 gtgatcgctt caagcgcctg ttcgagccct gcgcgcgcct tgacctcgac gaagatgagc
    13921 gttcccccgc gccgggcgac aagatcgacc tcgccgccgg cggcggcaaa acgacgcgcc
    13981 aggatgcggt atcccttggc ccgcaagaga agctccgcca gccattcggc atcaagcccg
    14041 aagccatagg cacgatgatg gcgggtcact ggccatcccc attacggccc tgcaacagca
    14101 gggcacgggc atagaccgcc cggcgcggca ggcccaggtc agcagccacc agggccacgg
    14161 catcgcgcgg gctgtgttcg gcaagcgcgg agcgcagccg tgcgtccacc acgtcgtcgc
    14221 cgggcgcgga ttcgccgcct gaggccgggg caatcaggat aacgatctcg ccgcggggct
    14281 tttccgaatg ggcataatgc gcagccagtt catcgagggt tccgcgccgg atcgtctcga
    14341 atttcttggt cagttcgcgc gcaaccgccg ccgggcgatt acccaggatg gcagcaagat
    14401 cggccaggct ttcatccagc cggtgtggcg cttcgaacag cacgattgtt gcatcgatcc
    14461 cggcgatctg ccgcgcacgg cggcgtcgtt cggccgaacg cggcggcaga aacccctcga
    14521 aaaagaatcg atccgatggc aatccggcca gaaccagcgc ggccagaacc gccgaggcgc
    14581 ctggcagggc cgtcaccgca tggccagcct ccaccgccgc ctgcaccagc ttgtaaccgg
    14641 ggtcggagac cagtggtgtg ccggcgtcgg ataccagcgc caatgccgct ccgccggcca
    14701 gacgcgccag aatgtcgggg cgcatgagtt cggcattgtg ctcgtgatag ctcaggatcg
    14761 gcgtagatat gccgtagtgg gcgagcagca ccttggtgac ccgcttgtcc tcggccagga
    14821 tcgcatctgc ggccgccagc gtttccagcg cccgcagtgt gatatccttg agattgccaa
    14881 tcggggtcgc aacgatgtag agccccggcg gacagcgggt cgtttcagcc tgcaggccga
    14941 gcagtgtgta ggtcgatgtc atggtgccaa ttgaggccga agcgtgccgg aaggcaagcg
    15001 gcctgcacaa gagagggcct gccgatccaa tgtcttcaat ggtttagcga tcgacgttaa
    15061 ccgctttggg acttttctgt cttcgcccat gcatcaacct gtgcagttct gttatcgtta
    15121 atgcaccgca ttttagcctt ttcatgctaa aggtggcgac gacactggtt ttttgtatcg
    15181 gcgagggttc catggtgacc gcactctgct ccgttcttct tgacactgac gtgcgtcgcc
    15241 ggatccgcaa atggaccggc gcctttggtg ttgcggcgct gctggcagcc tgcgccgatt
    15301 cctcctcggt ctttccgccg ctgaaccagc cgtcgacccc ctcggcgggc accagccagc
    15361 cgatctccgg caatacgatt ggtagcggcc cggtgcgtgt ggcgctgatc ctgccgctga
    15421 cggccgccaa cggcgcggtg ccggcccagt cgctgcgcaa tgccgcggaa atggcaattg
    15481 ccgagttcaa cagccaggac atgaccatca tggtcaagga tgatcgcggc acgccggaag
    15541 gcgcccaggc tgcggcgcag gaagccctgg cccagggtgc ggaagcaatc atgggaccgc
    15601 tgttcgctcc aacggtgcaa tcggtggcaa gcgttgcgcg tgcggcccag cggccggtta
    15661 tttcattttc gaccgacaca tcggttgctg cccgcggtgt ctatctgctc agcttcctgc
    15721 cgcaatccga tgtgcggcgg attgtcagct acgcagcttc gcgcggcaag aaatcctttg
    15781 cggccctgat cccgcagagc gcctacggca atgtggccga ggtggaattc acccaggccg
    15841 tggctgcggc cggcggcagc gtggtcgcag tcgagcgtta ccagccgacc aaggcttcgc
    15901 tcgatgcggc cgtcgccaag ttgaagggca gcatgggccg ggccgacacg cttttccttc
    15961 ccgatgacgg caacggcatt ccggtcgtcg ctgccgccct gcgcactgcg gggatcggtg
    16021 gcccctctgt ccagctcatc ggaaccggcc tgtggaacga ggagcgcgtg ctcaagacgc
    16081 ccagcatgtc gggcggctgg tttgccgcac cggccgggac gggtttccag agctttgccc
    16141 agcgttaccg cgcgcgcttc aattccgatc cgacgcggat tgccacgctg tcctatgatg
    16201 caaccgcgct gctcgccgcc ttggcacgca cgcaaggaac gcgccggttc accgaacagg
    16261 tgctgaccaa tccttcggga ttcagcggtc aggacggcgt gttccgcttc cgtgccgatg
    16321 gactgaacga acgcggcctg accatctatc aggtgaacaa tggcgctgcg atggtgctgc
    16381 aaccggcccc gtcaagcttc gggcaggccg gcggctgagg cttcgccgga ggcaggtcgg
    16441 tccggaaccg acctctgcct tccagcctca ggccgaatag gcctcatacc aagggtcatg
    16501 acaattgacc cttggttcct gttttgaatt ttcatttccc cttgaagaat aaggaaaatt
    16561 caaaattcgt catcggtcac tcgtcgtgac cgatggtatc aggcggcaag atcggcgacc
    16621 acggcatcaa gcaccggaaa tccctcggcg gtgacgcgca gccgtcctga ttcggtcacc
    16681 tcgaccatgc ggtgttcgac aagatcggcc acccgccggt catccagggt ccgaccgcga
    16741 agccgggcaa accgggcagg gtcgatccct tcgcgcaggc gcaggcccat cagcaggaat
    16801 tcgtcggcct gctgctcatc ggtgagaatg tcggcctcga cgccgccgtg gcccttggcc
    16861 tcgaccagcg acagccagcg ttcggggctc ttctcgttgg caatggcgat tcggccttct
    16921 ttggtcaccg cgcggccgtg ggcgccggcg ccaaggccgg catattcgcc atagcgccag
    16981 tagacgaggt tatgccggct ttcctcgccg ggccgagcat ggttggagat ttcataggcg
    17041 ggcaggccat ggtccgcgca caaagcctgc gttgcatcat agagcgcgcg tgccgcctcg
    17101 gtatcgggca cgacgagctt gccatcgctg gccagtcggt cgaacggtgt gtccggctcg
    17161 atggtgagct gatagagcga cagatgggtc ggtcccagat ccagagcccg gcgcagttct
    17221 gtctgccagc ctgaaattgt ctgcccaggg cgggcataga tgagatcaaa cgacgtgcgc
    17281 tcgaatgtgc ctcgcgcgat ggcaatggct gcaagcgcgt cctcgacgct gtggaggcgc
    17341 ccgagaaagg caagatcgac cgggttcagg gattgcacgc ccagcgaaac ccggttgaca
    17401 ccggcggctc ggtaaccgcg gaaacgtccc gcctcgacgc tggtcggatt ggcttccagc
    17461 gtgacctcga cattcccggc caccggccag gccgcgccga tcgcgtcgag aatcgcgcca
    17521 accgtcgccg gctgcatcag cgagggcgtg ccgccgccaa aaaagatgct ggagaccagg
    17581 cgtcccggcg tccgttccgc ccaggtggcg atttcccgcc ggaaggcggc gatgaagcgc
    17641 ggctcgtcga cggggtcacg gcggacatgg ctgttgaaat cgcaataggg gcatttggca
    17701 gcgcagaatg gccaatgcac gtagacggca aagccggcat cgaagggcgg ggccgcgatc
    17761 atgtgaccag ggccgcgatc atcgcggcga aagcgcggct gcggtgggaa tgccgcttct
    17821 tttcctccgg cagcaattcg ccgaacgtgc ggctctctcc gtcggcaaca aagatcggat
    17881 catagccaaa accattggat ccgcgtggcg gaaaggccag agtaccgaat acgcggccct
    17941 cgaacaccgc ctcgctccca tccggccagg cgacaaccac cgaagagaca aaatgcgcct
    18001 gttgacccgt cggggtcagg ccgcgtcgcg acagttccgc agcaatccgg gccatgccac
    18061 gcccgtagtc gcgcggctcg cccgcccagt cggcggtgaa aatgcccggt gcgccatcca
    18121 gggcatcgac gcagataccc gaatcatcgg caatagccac gatgccggtg gcagcggccg
    18181 cagcccgcgc cttgacgcct gcattctcgg aaaacatcgt gccggtctcg gcgggctcgg
    18241 ccagacccag ctgcccggcc gagacaacgt ccaccccatg gggagccaga agatcgatca
    18301 tctcgaccag cttgccggca ttgtgcgtgg cgatcaccac acggccgcca agacgacgga
    18361 gcgaaggggc cgctgccatg gtcaggccac cgccatcttc tgcaggtcga ccagtcggcg
    18421 aatgccaccc tgggcgagag acagcaggct gagaagctca ctctgactga acggcgcccc
    18481 ctctgctgtg ccctgcactt cgacgatgcc gcccgatccg gtcatgacga aattggcatc
    18541 cgtctcggcg ttcgaatcct cggcataatc gaggtcgagc accggaacgc ccctgaagat
    18601 accgcaggag acagccgcga catgatcctt cagcgggttg gtcttgacca gatcgcgcgc
    18661 ccgcatccag gcaaggcagt cattgagggc gacccaggcg ccggtgatcg aggctgttcg
    18721 cgtgcccccg tcagcctgaa tcacgtcgca gtcgagcaca atctgacgtt cgccgatcgc
    18781 cgcaagatcc acgaccgccc gcatcgaacg gccgatcagt cgctggatct cctgtgtccg
    18841 cccggagggc tttccagccg tgacctcacg ccgggtccgc tccagcgtgg cgcgcggcag
    18901 catggcatac tcggccgtga cccagccctt gccagagcca cgcagccatg gcggcccccg
    18961 ctcctccaga gaggcggtgc aaaggacatg ggtttcgccg aacttgatca ggcaggagcc
    19021 ttccgcatag cgcgcaaccc cccgctccat cgtgaccttg cgcaactcgt ccggcgcgcg
    19081 tttggatggc cgcatcgttt caccttcctt gatgggctgt gtgtaggcgt ttgcctgacc
    19141 gtgcacaagc gcgccagctt gccgccggtc acatcgggtg gcaacatcgc ggcagttgcg
    19201 ctgcggagag tcatgaccaa agaccggccg atcaaccctg ccacgccgct gcccgaggct
    19261 ttcgatgccc gggcgcgcga tattttccgc catatcgtcg acggttatct ggccaacggc
    19321 gaaccggtcg gttcgcgcca catttcgcgc cagttgcaga tgccgctatc gccggcctcg
    19381 gtccgcaatg tcatgcagga tcttgaggat ctcggtctga tctatgcgcc ccacaccagc
    19441 gccgggcgcc tgccgacgga actcggactg cggttgttcg tcgatgcgat gctggagatc
    19501 ggcgatgtct cggccgagga caaacggcag atcgaggcgc aggtgcgtgc cgccggcgca
    19561 ggagccccgg acaagttcga ggatgttctg gccgaggcct cgcgtctggt ctccggcctt
    19621 gcacgcggcg cgggtgtggt gatcgccagc aaggccggca agcgctggaa gcacatcgag
    19681 tttgtccgcc ttgatccttc gcgcgctttg ctggtgctgg tgggcgagga tggccagctt
    19741 gagaaccggc tgctgccact gccggccggc ctgcccgcct cggcgctgac gcaggcggca
    19801 aattacctca atgcccggct cggcggccgc tcgctcggcg aggtgcgcgc cgagatcgaa
    19861 acgcgccggc aggagatgga gcgcgagatc gatgcgttga cggcccggct gatcgacagc
    19921 ggacttgcca gcctcagcga gggcgccgac ggagcaaccc agctcatcgt tcgcggccag
    19981 gccaacctcc tggaggacgt gcgggccgcc gaggacctcg accgcatccg ccggctgttt
    20041 gccgatcttg aatcgaaaaa ggacctgatc gatctgctga cgcgggccga aagcgccgac
    20101 ggcgtgcgca tcttcatcgg ctctgagaac aaactgttct cgctgtccgg ctcatcgttg
    20161 attgccgctc cgttccgcga cagccagcag cgcatcgtcg gcgtactcgg cgtcatcggt
    20221 cccacacggc tcaactacgc ccgcatcgtc ccgatgatcg attacactgc ccaggttatc
    20281 ggccgcatgc tcgacaggcg gcggtgatcg cgcagcctaa ggcgcgtggg gattcatcgt
    20341 gaattgatga cggctgctgc gatgtaga
//
LOCUS       DAOMXG010000025        19687 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_1624_length_19687_cov_11.732681, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000025 DAOMXG010000000
VERSION     DAOMXG010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 19687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19687
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1624_length_19687_cov_11.732681"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..1331)
                     /gene="aceA"
                     /locus_tag="PLQ11_03115"
     CDS             complement(57..1331)
                     /gene="aceA"
                     /locus_tag="PLQ11_03115"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019997742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HRK23923.1"
                     /translation="MTASYAPENKRDFTKADVEKLSGSFKMEHTLAKMGAARLRYLLA
                     NRPFVRTLGALTGNQAMQQVKAGLEAIYLSGWQVAADANNAGQMYPDQSLYPASSVPD
                     VVRRINNTLRRADQIAHMEGDKSVHFMAPIIADAEAGFGGPLNAFELMKAMIEAGASG
                     VHFEDQLASEKKCGHLGGKVLVPTKTFIRTLNAARLAADVCGVDTVLLARTDAHAANL
                     ITSDVDDYDHPWIDRNSRTEEGFFRLKQGNKHSVEHAIARGLAYGAYADLVWWETSEP
                     DLGEAREFATEFKKKYPNKYLAYNCSPSFNWRKKMTDAEAAKYQDEIAAMGYRYQFVT
                     LAGFHSLNLSMFELAKGYSKRGMGAYSEMQQREFAAADIGFTAVRHQREVGTGYFDAV
                     AMAISQGKSSTTALAGSTETEQFHDGKKSAAE"
     gene            1540..2967
                     /locus_tag="PLQ11_03120"
     CDS             1540..2967
                     /locus_tag="PLQ11_03120"
                     /inference="COORDINATES: protein motif:HMM:NF021382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain fatty acyl-CoA regulator family
                     protein"
                     /protein_id="HRK23924.1"
                     /translation="MRAVRIGGRLRRLRQEMRLNQAQMAAELNISPSYLNLLESNQRP
                     VTVNILLRLAERFHVDLKSLGGEDDARLTSDLMEALADPLFEGQDVKTSDVRDLVSTL
                     PSVGKALVALYHAYQRGGAAAANRSDDMDGESPGSALPSEEVTDFLQRRMNHFPELEE
                     CAETLWVENGLALFTLQQDLVKVLNQRFAVDVEIAPAATLPGLLRSYNPLTRRLVLSE
                     MLPLSSRTFQLAHQIAYLGYRREIETVVSGGKFSTAEADSLARSALANYFAAAVMAPY
                     GRFLEAARSTRYDLDTLQHRFGISFEQACHRLTTLRRPGAEGIPFHLIRVDIAGNISK
                     RFSASGIHIARFGAACPRWNVYDAFATPGMLRVQVSQMPEGTAFFCVARTISTAGRLI
                     SRGGLPNRVGQLSIGLGCAAHYARDIVYADGLNLDDPQIVTPIGVSCRTCPRTDCADR
                     AMPSMAQRLEIDENRRGLSTYALAV"
     gene            3057..3653
                     /locus_tag="PLQ11_03125"
     CDS             3057..3653
                     /locus_tag="PLQ11_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23925.1"
                     /translation="MPPTVLRPDPANSTLELGAMFFMSLAALGAAFMMPPEDGGGGTR
                     FLIGFAGVAGLLLSSLLNNRLQRGDMRIELHDDTLIYFNPDARRIDRDTIIEAVLETE
                     RDTDGVTYLTAIRHRDGASDEWSPVEKTVLPFGTLWDHSATVDAINRWLKSPVPAADA
                     RASGACLNFVPAKQRSQAAPMQVPPKGTIGRRHFGLRH"
     gene            3735..4691
                     /gene="tal"
                     /locus_tag="PLQ11_03130"
     CDS             3735..4691
                     /gene="tal"
                     /locus_tag="PLQ11_03130"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HRK23926.1"
                     /translation="MPSKLDQLKAMTTVVADTGDIASIKVFQPTDCTTNPTLILKAAQ
                     MPAYAGIVDEALNWAKTQGGSSEAIVARAADRLAVSFGAELTQIVPGRVSTEVDADLS
                     FDTARTVEKARAIIADYAARGIDRSRILIKIAATWEGIRAAEVLQKDGIDCNLTLIFS
                     LVQAVACAEAGTFLISPFVGRILDWHVKATGQTYTAETDPGVVSVRGIFDYFKTHGHR
                     TVVMGASFRNTGEIEALAGCDRLTIAPALLEHLQADTAPLQRALDPARPATAPVRIAT
                     DEAAFRWQLNEDAMATEKLAEGIRLFAKDLAALRAMLSTRLG"
     gene            4792..5310
                     /locus_tag="PLQ11_03135"
     CDS             4792..5310
                     /locus_tag="PLQ11_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HRK23927.1"
                     /translation="MSALASRSWSPYVAGIVIGLLQIPAFLLMGTALGTSSSYVTISA
                     SIAQVFDPSIAEIKYAANHLTGAKNWWQVALIGGIVVGAFLSSRLSGTGRWGASPVWA
                     RAMGAHTPAQRYLMAFTGGFIMVLGARIADGCTSGHGVSGIAQLAIGSFVAVTAMFGA
                     GIVAANLIYRRV"
     gene            5310..5927
                     /locus_tag="PLQ11_03140"
     CDS             5310..5927
                     /locus_tag="PLQ11_03140"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HRK23928.1"
                     /translation="MSVLAAMLLGLAMGIVFGFALEKGRVCEAGVIVCQMQMSRHAML
                     KMFLSAVITGLLVLAFLVGFGFAKLSPKATLFAADIIGGAILGIGIVVAGACPGTVLA
                     QIGTGYKDAIFTFIGGLFGALAFTYAEPTLLKPILNAGSYGKLTLDQLLGVPFPALAL
                     GFAAVLAGLLIWLEKLQPWQEEIGPAGDGLPAEEPRRDAVPAPTA"
     gene            5990..7795
                     /gene="lepA"
                     /locus_tag="PLQ11_03145"
     CDS             5990..7795
                     /gene="lepA"
                     /locus_tag="PLQ11_03145"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018262923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HRK23929.1"
                     /translation="MSTHPIDNIRNFSIVAHIDHGKSTLADRLIQHTGTVALRDMSEQ
                     MLDSMDIERERGITIKAQTVRLDYKAEDGRAYILNLMDTPGHVDFAYEVSRSLAACEG
                     SLLVVDASQGVEAQTLANVYHALDAGHEIVPVLNKIDLPAAEPERIKEQIEEVIGLDA
                     SDAVPISAKTGIGIDLVLEAIVKRLPPPKGDEAAPLKALLVDSWYDVYLGVVVMVRII
                     DGVLRKGSDITMMGTKARYQVDKIGVFKPKMVDVEKLGPGEVGFITAQIKQVADTRVG
                     DTITDTRKPTAQALTGFKPAQPVVFCGLFPVDAADFEDLRSAMGKLRLNDASFSYEME
                     TSAALGFGFRCGFLGLLHLEIIQERLEREFNLDLIATAPSVIYKLALKDGADIELHNP
                     ADMPEVMKIEEIQEPWIKATILTPDEYLGGILKLCQDRRGNQIDLNYVGKRAMVVYEL
                     PLNEVVFDFYDRLKSISKGYASFDYTLTDYKPGDLVKMSILVNDEPVDALSMLVHRTR
                     AEGRGRAMCEKLKDLIPQHLFKIPIQAAIGGKIIARETISALRKDVTAKCYGGDVTRK
                     RKLLDKQKEGKKRMRQFGKVDIPQEAFIAALKMDT"
     gene            7914..8402
                     /locus_tag="PLQ11_03150"
     CDS             7914..8402
                     /locus_tag="PLQ11_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017485748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HRK23930.1"
                     /translation="MTCQAGLRTKGGQRRLALGALLACAVAVSAPALANDTIDVQMDS
                     AKLLKMPEGADTIVIGNPMIADVSLQRNNVLVVTGKQFGTTNLIALDRAGAIISESRV
                     VVMSPQKDRLVVYRGLQQETYSCNPDCGAVTQIGDAEAHFGRSRSQSTDRLQAIRSSS
                     GN"
     gene            complement(8843..9823)
                     /locus_tag="PLQ11_03155"
     CDS             complement(8843..9823)
                     /locus_tag="PLQ11_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HRK23931.1"
                     /translation="MTAKSITPDTLRRDCPAEKFRLITRSDMDGLVCGTLLKELGLVE
                     RIDFVHPKDMQDGKIAVGAGDISTNLPYVPGIHVAFDHHESEGKRIADKPANHIIDVK
                     APSAARVVYDFFGGKAVFPNISDAMMVAVDKADAARFNRDDVLDAKGWELLSFLMDAR
                     TGLGRFRQFRISNYQLMMMLIDACRQHASIEEILALPDVRERVDLYKAHEELSRTQIQ
                     RCTRIEHPLAVLDLRGEETIYASNRFMIYALFPDATISCHVMWGREKQNTVLAFGKSI
                     FDRSNRTNIGELMLEFGGGGHAAAGTCQIANDKAELALAALSQRITKDAA"
     gene            complement(10078..10929)
                     /locus_tag="PLQ11_03160"
     CDS             complement(10078..10929)
                     /locus_tag="PLQ11_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23932.1"
                     /translation="MSKTRWKAFPHASSDFDYKGPALAKAWARLHQGDCEPFPDAKTL
                     EAQAKAHPRLKPKGSFDDAAATVQDAWRAYHRGDFGGAVELGLSVGLLGYNVANKATN
                     IYASYLEASDSAKLALLQESASRAEELIGAAKSLANAWYLHAHALGRYSQGISIGKAL
                     AEGLAGKVRNSLEQAISLAPKHADAHIALGTYHAEIINKVGGMIGGLTYGASKDAGVK
                     HFETALKLNPTSAVARTEFGAGLIMMFGNSKMKDALKLYEQAASCEPKDAMECLDVEA
                     AKEELED"
     gene            complement(10945..11589)
                     /locus_tag="PLQ11_03165"
     CDS             complement(10945..11589)
                     /locus_tag="PLQ11_03165"
                     /inference="COORDINATES: protein motif:HMM:NF025338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRK23933.1"
                     /translation="MERKPPRRTKERILATALDLFNRFGERAVTTSAIAEDMRISPGN
                     LYYHFKSKEMIVEALFGLFRQEIEHMLAAPEKRPSETEDIWLFLHLVFEAIWKYRFIY
                     RDLTDLVSRHRPLEVQFKRILAHKQRTAEAIMHGLVQGGRMHASRADIEALALNMTLI
                     ATYWLSFDYVRHPRAPDDGTTLARGAFHVMALAAPYLTPHERALLDHLAQRYKS"
     gene            complement(11634..12854)
                     /locus_tag="PLQ11_03170"
     CDS             complement(11634..12854)
                     /locus_tag="PLQ11_03170"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRK23934.1"
                     /translation="MKRRNRKAIGVALAGGGPLGAIYEIGALVALQDALRGVDLVNCD
                     VYVGVSSGGVIATGLANGLTPRDMYRMFIADEAAEHAFDPDILLRPAFAEYARRLAAI
                     PDLCLQAARRYLENPLSYGFFESFQRLARAVPTGLFDNNAFSAFLTRLLSGPGRTNDF
                     RELRHKLFLVATDLDSGKAVPFGAPGWDHVPIARAVQATTALPGLFPPVEIDGRYYVD
                     GALMKTLHASVALKEGASLLFCVNPLVPFNASLAHDEGFSGGFSLMDHGLPAVMSQTF
                     RSIIHSRMKTGMDRYRTEFPDADVLLFEPGQGDVEMFFTNVFSYASRKRLAEHAYQRT
                     LADLRRRREQLEPVLARHGVELDMYVIGARRQSIASGPSSALHGRDLREATLELDQTL
                     DALNAVLKTRLPAK"
     gene            complement(12977..13597)
                     /locus_tag="PLQ11_03175"
     CDS             complement(12977..13597)
                     /locus_tag="PLQ11_03175"
                     /inference="COORDINATES: protein motif:HMM:NF017415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HRK23935.1"
                     /translation="MAKKLKALAGGSNETHLAQTIKDSAHQIWLAGLGAFAKAQEEGT
                     KVFEALVKEGEKIQTKTREVAEDKIAEARKAASGTWDKLESVFEERVARALHSLNVPT
                     KKDIDLLTQRVHELTASTKKLSGTVQTEAKKTSQSVSAKAQKVLKQASAATDEAAEVV
                     STSAKKVLKQAGAVKDDAAELVTTSAKIAKDEAAKVIASVKAKTVG"
     gene            13780..15765
                     /locus_tag="PLQ11_03180"
     CDS             13780..15765
                     /locus_tag="PLQ11_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRK23936.1"
                     /translation="MHYVVTGASGFIGKRLVRALLDRPEAVVYFVMRNPTAERVGALR
                     AFWNVDAKRAVPIEGDLTQPVLGVAKSNIKKLTGKIDHVFHLAAIYDLEADAETEMAA
                     NVDGTRNAVKFAEAVGARCFHHVSSIAAAGMYDGVFREDMFVEATKLDHPYFASKHIA
                     EKVVRSECSIPWRVYRPGIVVGDSKTGEMDKIDGPYYFFKLIQKMRRMLPPWMPTIGI
                     EGGRINIVPVDFIVAALNHLSHLKGHDGKCFHLTDPEPRRVGDILNIFARAAHAPEMA
                     ARVNASLFSFVPDAVMKSLMALAPVRRIKNAVMKDLGLPPDMFRFINYPTRFDCREAE
                     KLLKPAGITVPPIETYAWRLWDYWERHLDPDLFIDRSLHGKVGGKVVVITGGSAGIGK
                     ATAFKIAGAGGRTIIVARDAEKLAAAAKEAEAQGLKLFTYVGDVSSPEQCDTFIKKVL
                     KDHGHVDILINNAGRSIRRGIENSYDRFHDFERTMQINYFGALRLTLGLLPSMSERRK
                     GHVINISSIGVLTNAPRFSAYVASKAALDAWTRCAASEYLDRGVEFTTINMPLVRTEM
                     IAPTKFYQNVPTLSPDEAADLIVEAIIHKPSRVATRLGIFGQVLHAVSPRVAQIIMNT
                     SFRMFPDSEAAKGAKPGEEMPTAEQVTMTQIMRGLHL"
     gene            complement(15805..16692)
                     /locus_tag="PLQ11_03185"
     CDS             complement(15805..16692)
                     /locus_tag="PLQ11_03185"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRK23937.1"
                     /translation="MLAWLLRGLLLGQALALTGLFYTYLGFGWVAGAAASVLAFLVLQ
                     GLVLALAYVVSQLYASPRPPGIGPLTGAAIFQEYCGFLAFFSAIQPFERLFMGPDAVG
                     RLPTGQRPVLLVHGYMCNRGYWWWFRSALRARGHAVATISLETPFTDIEEMAQQLDRR
                     IESLIEETGADMVLLVTHSMGGLVARTYLRRFGSSRIARFITLGGPHQGTVVATFGLG
                     RNARQMEPDNDWLAELNRNWSPPVPTLSVWSTGDQIIAPQDSSRLPHTAERVFPAIGH
                     LALAIEPDVFEAVASELAS"
     gene            17224..17781
                     /locus_tag="PLQ11_03190"
     CDS             17224..17781
                     /locus_tag="PLQ11_03190"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HRK23938.1"
                     /translation="MPESRAGTRRPAGRFARFRRDERGATAIEFGMISLPFFAILFAI
                     AETGIAFLNGQFLDRAVEKASRKIFTGQLVNGQANPQARFQQFQQEICATVNAFFSCG
                     NLMYDVQAYRTFGDTMLPIPMVNGDLDASALPRFNPGRSGEVVIVRVYYRVPVYADLL
                     GAGMGNLAGNKRLLVGTAAFKNEPY"
     gene            17781..18455
                     /locus_tag="PLQ11_03195"
     CDS             17781..18455
                     /locus_tag="PLQ11_03195"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HRK23939.1"
                     /translation="MLSLSFARSLAAVMPKAKALARNPLNRLARRRLRAFGKAKSGIA
                     ATEFALVVPLMLTMYFGMVELTQALGHARKSVIMSRTLADLVTQSVGISDTDMNTIFT
                     AARSVMAPYPDDRLAMRVTSVMIDGAGTAWVEWSDVKNNNATNPYSQHARCALANSFI
                     PASLRVPRTYLVWSEVTIQHIPTIGHVVAPNGFTMTEKMPMRPRVSTSIGRQNVPGTS
                     CPAAVP"
     gene            18608..19213
                     /locus_tag="PLQ11_03200"
     CDS             18608..19213
                     /locus_tag="PLQ11_03200"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRK23940.1"
                     /translation="MGDIAVFDVGNVLVRWDPRNLFQKVFADPERMDFFLSEVCSPEW
                     NHAQDAGRMWAEAEAEAIARHPAFAREVRLFRGRWHEMIDGPVEANVRLLEGLRRRGV
                     ATFAITNFAADTFAETQERYPFLKAFDGIVCSGLEKICKPDPAIYRLLLDRYRLVAKD
                     CVFIDDSAANIAAAAALGFRTVHYGLGLDAAGAFAQLGLPV"
     gene            complement(19284..>19687)
                     /locus_tag="PLQ11_03205"
     CDS             complement(19284..>19687)
                     /locus_tag="PLQ11_03205"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HRK23941.1"
                     /translation="TPAAAQPAPPGPQAVALAADESGHFKADIRINGQFLKGLLDTGA
                     TVIAIPADEARKLGITPAASAYTSPVQTANGVVQAARVKLNEVRIGSIVVRDIEALIV
                     PKGLPVTLVGMNFFTRLQSFEIRGKSLVLRQ"
BASE COUNT         3666 a         6099 c         6048 g         3874 t
ORIGIN      
        1 ccggatcgcc ccttgtcccg gcctgtcggc cgcaacagag ggccctggct tttttattac
       61 tcggcagccg acttcttgcc gtcgtggaac tgctcggtct cggtcgagcc ggcaagcgcg
      121 gtcgtggacg acttgccctg cgaaatcgcc atggcgaccg catcgaagta gccggtgcca
      181 acctcgcgct ggtgacggac ggcggtgaag ccgatgtcgg ccgcggcgaa ttcgcgctgc
      241 tgcatctccg aataggcgcc catgccgcgc ttcgaatagc ccttggccag ttcgaacatc
      301 gacaggttga gcgagtggaa gccggcgagc gtgacgaact ggtagcgata acccatcgcg
      361 gcgatctcgt cctgatactt cgccgcctcc gcgtcggtca tcttcttgcg ccagttgaac
      421 gacggcgagc agttgtaggc cagatacttg ttcggatact tcttcttgaa ttcggtcgcg
      481 aactcgcggg cttccccgag atccggctcc gaggtctccc accagacgag atcggcatag
      541 gcgccgtagg cgaggccgcg ggcaatcgca tgctcgaccg agtgcttgtt gccctgcttg
      601 aggcggaaga agccttcctc ggtgcgcgag ttgcggtcga tccagggatg atcgtagtca
      661 tcgacatccg aagtgatgag gttggccgca tgggcgtcgg tgcgggccag cagcacggtg
      721 tcgacgccgc agacgtcggc ggcgaggcgg gcggcattca gggtgcggat gaaggtcttg
      781 gtgggaacca gaaccttgcc gccgaggtgg ccgcacttct tttcagaagc gagctggtcc
      841 tcgaagtgga cgcccgaggc gccggcctcg atcatcgcct tcatcagttc gaacgcgttc
      901 agcgggccac cgaaaccggc ttccgcgtca gcgatgatcg gcgccatgaa atggaccgac
      961 ttgtcgcctt ccatatgggc gatctggtca gcgcggcgca gggtgttgtt gatgcggcgc
     1021 accacatccg gaaccgacga ggccggatac agcgactggt ccggatacat ctggccggcg
     1081 ttgttggcgt ccgccgccac ctgccagccc gagagataga tggcttcgag gccggccttg
     1141 acctgctgca tcgcctggtt gccggtcagc gcgccaagcg tgcgcacgaa cggccggtta
     1201 gccagcaggt agcgcaggcg ggcagcgccc atcttggcca gcgtgtgctc catcttgaag
     1261 gagcccgaca gcttctcgac atcggccttg gtgaaatcac gcttgttttc gggagcgtag
     1321 gaggcggtca tcgcaatcct cataggtggg tcaaaccgga gcaggcgaag gcgagccctc
     1381 accctgcggc gtggcgcttc catgcacaaa aacgcgctgc aatgcaataa gacattgaat
     1441 taacgagaaa aagcaggaaa atcatcaaca gattgacaaa tgtaaatttg taaattgtaa
     1501 taccattgta aattctcgtg cagattcaag gggccggcaa tgcgggcggt gcggattggc
     1561 ggtcggttgc gccgcctgcg acaggaaatg cggctcaatc aggcccagat ggccgccgaa
     1621 ctgaacatat cgcccagcta tctcaacctg ctggagagca accagcgtcc ggtcaccgtc
     1681 aacatcctgc tgcggctggc cgaacgattc catgtcgatc tcaaatcgct ggggggcgag
     1741 gatgacgcgc gcctgacctc ggacctgatg gaagccctgg ccgatccact gttcgagggc
     1801 caggacgtca agacctccga tgttcgcgac ctggtatcca ccctgcccag tgtcggcaag
     1861 gcgctcgtcg cgctctatca cgcctaccag cgtggcggag ccgccgcggc gaatcgttcc
     1921 gatgacatgg acggagaaag tcctggttcg gcgctgccct cggaagaggt gaccgacttc
     1981 ctgcaacggc gaatgaacca tttccccgag ctggaagaat gtgccgagac cctgtgggtg
     2041 gaaaacggcc ttgccctgtt caccctgcag caggatctgg tgaaggtgct gaaccagcgt
     2101 ttcgccgttg atgtcgaaat cgccccggcg gcgacattgc ccggcctgtt gcggtcctac
     2161 aacccgctga cccgaaggct ggtcctgtcg gaaatgctgc cgctgtcgag ccgcaccttc
     2221 cagctggctc accagattgc ctatctcggc tatcgccgag agatcgaaac ggtggtttcg
     2281 ggtggaaaat tctccaccgc agaagccgat tcgctggcgc gctcggcact cgccaactat
     2341 tttgccgccg ctgtcatggc gccttatggc cgtttcctcg aggcggcgcg ctcaacccgc
     2401 tatgatcttg acacgctgca gcaccgattt ggcatttcct tcgagcaggc ctgccaccgg
     2461 ctgaccaccc tgcgtcggcc gggcgccgag ggcattccat tccatcttat ccgggtcgat
     2521 atcgccggta atatctccaa acgattttcg gcttccggca tccatatcgc ccgtttcggc
     2581 gccgcctgcc cacgctggaa cgtctatgat gccttcgcaa cgcccggaat gctgcgcgtc
     2641 caggtttcgc agatgccgga agggaccgcc ttcttctgcg tggctcggac catttccact
     2701 gccggccggc tgatctcgcg cggtggcctg cccaaccggg tcgggcaatt gtcgatcggc
     2761 ctcggttgcg cagcccatta cgccagggac atcgtctatg ccgacgggct caatctcgac
     2821 gatccgcaga tcgtcacgcc gatcggtgtc tcctgccgca cctgcccgcg caccgattgc
     2881 gccgaccggg cgatgccgtc gatggcgcag cgtctggaaa tcgacgagaa ccgacggggc
     2941 ctgagcactt acgcactggc cgtttagtcc ccgttaggga gccgtacagg ctctttcggc
     3001 acgttaagga cgcctttatc acggcccggc accatcggcc cgaaggagcc atattcgtgc
     3061 caccaaccgt tcttcgcccg gatcctgcca acagcaccct cgaactgggc gcaatgttct
     3121 tcatgtcgct cgcagccctt ggtgccgcgt tcatgatgcc tcccgaggat ggcgggggag
     3181 gaacacgttt ccttatcgga tttgccggcg tggcgggcct gctgttgtct agcctgctga
     3241 acaatcggct tcaacgtggc gacatgcgca tcgaacttca cgatgacacg ctgatctatt
     3301 tcaatccaga cgcccgacgc atcgacaggg atacgatcat cgaagccgtg ctggaaaccg
     3361 aacgtgacac cgatggtgtg acctatctga ccgccatccg tcaccgtgac ggggcatcgg
     3421 atgaatggtc ccctgtcgaa aagaccgtcc tgccgtttgg cacgttgtgg gaccattcgg
     3481 ccacggtcga tgccatcaac cgctggctga agtcgccggt gcctgccgcc gatgcccggg
     3541 cgtccggcgc atgcttgaat tttgtcccgg caaagcagcg gtcccaggcc gcacccatgc
     3601 aggtgccgcc aaagggcact atcggtcggc gtcatttcgg cctgcgtcac tgaaacggct
     3661 gagctaagac gcctgttgct ggtgcccccg gcaggctgta agcaaacaat ctccccttca
     3721 ttgcagcaag caacatgccc tccaaactcg accagctcaa agccatgacc acggttgtcg
     3781 ccgataccgg cgacatcgcc tcgatcaagg tgttccagcc gaccgattgc accaccaatc
     3841 cgaccctgat tctcaaggca gcacagatgc ccgcctatgc agggattgtc gatgaggccc
     3901 tgaactgggc gaagacacag ggcggcagca gcgaggcgat tgtcgcccgc gccgccgacc
     3961 ggctggcggt cagcttcggc gcggagctga ctcagattgt gccggggcgg gtttcgaccg
     4021 aggtcgatgc ggatctgtca ttcgatactg ctcgcacggt cgaaaaagcc cgcgcgatca
     4081 tcgccgatta tgcggcgcgc ggtatcgaca ggagccgcat cctgatcaag atcgcggcga
     4141 cctgggaagg tattcgcgct gccgaggtcc tgcaaaagga cgggattgac tgcaatctga
     4201 cgctgatctt cagcctcgtt caggcggttg cctgcgccga ggccggtacg ttcctgatct
     4261 cgccctttgt cggacgcatc ctcgactggc acgtcaaggc gaccggccag acctataccg
     4321 ccgagaccga tcccggcgtc gtctcggtgc gcggcatctt cgattatttc aagacccacg
     4381 gccaccgcac ggtggtgatg ggcgcgtcct tccgcaacac cggcgagatc gaggcgctgg
     4441 ccggctgcga ccggctgacc atcgccccgg cgctgctcga acatttgcag gccgatacgg
     4501 ccccattgca gcgcgcgctc gatcccgccc gtccggcgac ggcaccggtc cggattgcca
     4561 ccgacgaagc cgccttccgc tggcagctca acgaggatgc aatggcaacc gaaaagctgg
     4621 ccgaaggcat ccgtctgttc gccaaggatc tggcggccct gcgcgccatg ctgtccacaa
     4681 gactgggctg atcgccgcgc gcagccggcg atatggtgtg ccgaggttct gcgcttgtct
     4741 atattagtat acttgaatat aagtggctgc gttgctacca cggagcctcc catgtccgct
     4801 ctcgcctccc gatcctggtc gccctatgtg gccggcatag tcatcggact tctgcagatt
     4861 cctgcctttc tgttgatggg cacggcgctc ggcacgtcct cgtcctacgt gacgatttcc
     4921 gcctcgatcg cacaggtttt tgatccgtcc attgccgaga tcaaatatgc cgcaaaccat
     4981 ctgaccggcg ccaagaactg gtggcaggtt gcactgatcg gcggcatcgt cgtcggcgcc
     5041 ttcctgtcct cacgcctgtc gggaacaggc cgctgggggg cctcgccggt gtgggcgcgg
     5101 gcgatgggag cccatacgcc ggcgcagcgc tatctgatgg cgtttactgg cggcttcatc
     5161 atggtgctcg gcgcgcgcat cgccgatggc tgcacctcgg gccatggcgt gtcggggatt
     5221 gcgcaactgg ccatcggctc gttcgtcgct gtcaccgcca tgttcggcgc cggcatcgtc
     5281 gccgccaacc tgatctaccg gagggtgtga tgagcgttct tgctgccatg cttcttggcc
     5341 ttgccatggg tatcgtcttc ggctttgcgc tcgaaaaggg ccgcgtctgc gaggccggcg
     5401 tcatcgtctg ccagatgcag atgagccgtc atgccatgct gaaaatgttc ctgtcggccg
     5461 tcatcaccgg cctgctggtg ctggcatttc tcgtcggctt cggctttgcc aagctgagcc
     5521 cgaaggcgac gttgtttgcc gccgacatca tcggcggcgc gatcctcggc atcggtattg
     5581 tcgttgccgg cgcctgcccc ggcacggtgc tggcgcagat cggaaccggc tataaggatg
     5641 cgatctttac cttcatcggc ggcctgttcg gcgcgctcgc cttcacctat gccgagccaa
     5701 ccctgctcaa gccgattctc aatgccggtt cctacggcaa gctgacgctc gaccagctgc
     5761 tcggcgtgcc gtttccggcc ctggcgctgg gctttgcggc ggttcttgcc ggtcttctga
     5821 tctggcttga gaagctgcaa ccctggcagg aggagatcgg acccgccggc gacggtctgc
     5881 cggcggagga gccacggcgc gatgccgtgc cggccccgac tgcctgaccg gaagcgggtt
     5941 cggcggcttt tgcttttctc atgcgcgcga aactgctagc agcgcgcgca tgagcaccca
     6001 tccgatcgac aacatccgca atttttccat cgtcgcgcat atcgaccatg gcaagtcgac
     6061 gctcgccgac cggctgatcc agcacaccgg tacggtcgcc ttgcgcgaca tgagcgagca
     6121 gatgctcgat tcgatggata tcgagcgcga acgcggcatc accatcaagg cgcagactgt
     6181 ccggctcgat tacaaggccg aggacggcag ggcctatatc ctcaacctga tggacacgcc
     6241 aggccacgtc gacttcgcct atgaggtgag ccgctcgctc gccgcctgcg aaggctcgct
     6301 gctggtggtc gatgcctcgc agggcgtcga agcgcagact ctcgctaacg tctatcacgc
     6361 gctcgatgcc ggccatgaaa tcgtgccggt gctcaacaag atcgacctgc ccgccgccga
     6421 gcccgagcgg atcaaggagc agattgagga agtgatcggc ctcgacgctt cagacgccgt
     6481 gccgatctcg gccaagaccg gcatcggcat cgatctggtg ctggaagcga tcgtcaagcg
     6541 cctgccgccg cccaagggcg acgaggccgc gccgctgaag gcgctcctgg tcgacagctg
     6601 gtatgacgtc tatctcggcg tcgtcgtcat ggtccgcatc atcgacggcg tgctcaggaa
     6661 ggggtcggac atcacgatga tgggcacgaa agcccgttat caggtcgaca agatcggcgt
     6721 gttcaagccc aagatggttg atgtcgagaa gcttggcccg ggcgaggtcg gcttcatcac
     6781 cgcccagatc aagcaggtgg ccgacacccg cgtcggcgat acgatcaccg acacccgcaa
     6841 gccgacggcc caggcgctga ccggtttcaa gccggcccag ccggtggtgt tctgcggcct
     6901 cttccccgtc gatgccgccg acttcgagga cctgcgctcg gcgatgggca agctgaggct
     6961 caacgacgcc tcgttctcct atgaaatgga aacctccgcc gcgctgggtt tcggtttccg
     7021 ctgcggcttt ctcggcctgc tgcaccttga aatcatccag gagcggctgg agcgcgagtt
     7081 caacctcgac ctgatcgcca ccgcgccgag cgtcatctac aagctggcgc tgaaggacgg
     7141 cgccgatatc gaactgcaca acccggccga catgccggag gtgatgaaaa tcgaggaaat
     7201 ccaggagccg tggatcaagg cgacgatcct gacgccggac gaatatctcg gcggcatcct
     7261 caagctctgc caggaccggc gcggcaacca gatcgacctc aactatgtcg gcaagcgcgc
     7321 catggtggtc tacgaactgc cgctcaacga ggtggtgttc gatttctacg accggcttaa
     7381 gtcgatttcg aagggctatg cctcgttcga ctacacgctg accgactaca agccgggcga
     7441 cctcgtcaag atgtcgatcc tggtcaatga cgagccggtg gacgcgctgt cgatgctggt
     7501 gcaccgcacc cgcgccgaag gccggggccg cgccatgtgc gagaagctga aggacctcat
     7561 cccgcagcac ctgttcaaga tcccgatcca ggcggcgatc ggcggcaaga tcatcgcccg
     7621 cgagacgatc tcggcactgc gcaaggacgt gaccgccaag tgctatggcg gcgacgtgac
     7681 ccgcaagcgc aagctgctcg acaagcagaa agagggcaag aaacgcatgc ggcagttcgg
     7741 caaggtcgac attccgcagg aagccttcat cgccgcgctg aagatggaca cctgatctcc
     7801 gattgaagcc aatcctgccc gccccttagc gttttgttca ccaagatcgc agacactgcc
     7861 tgagcgtggg acccggtgcg gtccgcatgg ctgcgcgatt gtggagagcg aacatgacct
     7921 gtcaggctgg gttgcggacg aaaggcgggc agcgccggct ggctctcggt gcgctccttg
     7981 cgtgcgctgt tgctgtttca gctccggcgc tggccaatga cacgatcgac gtgcagatgg
     8041 acagtgccaa gctgctcaag atgccggaag gcgccgacac catcgtgatt ggcaatccga
     8101 tgattgccga cgtctcgttg cagcggaaca acgtcctggt tgtcaccggc aagcagttcg
     8161 gaaccaccaa tctcatcgcg ctcgacaggg caggcgcgat catttccgaa tcgcgcgttg
     8221 tggtgatgtc accccagaaa gaccggcttg tggtgtatcg tggcctccag caggaaacct
     8281 attcctgcaa tcccgactgc ggcgccgtaa cccagatcgg tgacgccgaa gcccatttcg
     8341 ggcgttcccg gtcgcagagc accgatcggt tgcaggcgat tcgcagcagc tccggcaact
     8401 agagcatgtc gcgattaagt ggataccggt taatcgcaga agacatgctc taacttattg
     8461 ataccgatca cgttatccgc attctgatga ttccatcaaa atgcggcgtg atctaaagcc
     8521 tgctgcgaaa aagtggcaac ggtttttcgc gggaatcatg ctctaaacta ttgtaaccga
     8581 cgtaatcgct cgttttgatg gtcgcatcaa aatgagagcg taatctagag cattttcaag
     8641 cgaagtggag cccggttcgc gcaaagaaaa tgcgacaaaa caaaaagata gagcactttt
     8701 tgtgaattgg aattcactga aagcgctctg gcaaggcgag gcgaccaaac tccgggcagc
     8761 tgtgaagacc ccgcccctga tcgctcaagg gcaagggttt cgcagatcgg tggatggtcc
     8821 gacgatgttg ggccagtacg cgtcaggcgg catccttggt gatgcgctga ctgagagcgg
     8881 caagcgccag ttcggccttg tcattggcga tctggcaggt tccggccgca gcatggccac
     8941 cgcctccgaa ttccagcatc agttcgccga tattggtgcg gttggaacgg tcgaagatcg
     9001 acttgccgaa ggccagcacc gtgttctgtt tctcgcggcc ccacatgaca tgacaggaga
     9061 tggtggcatc gggaaacagc gcatagatca tgaagcggtt tgaggcgtag atggtctcct
     9121 ccccgcgcag atccagcacc gccagcgggt gctcgatccg cgtgcaccgc tggatctgcg
     9181 tacgcgacag ttcttcatgc gccttataga gatcgacccg ctcgcgcacg tccggcagcg
     9241 ccagaatctc ctcgatcgag gcgtgctgac ggcaggcgtc aatgagcatc atcatcaact
     9301 ggtagttcga gatgcggaac tgccggaagc ggccgagacc tgtgcgggca tccatcagga
     9361 aggacaggag ttcccagccc ttggcatcga gcacgtcgtc acggttgaac cgggcggcgt
     9421 cggccttgtc gacagccacc atcatcgcat ccgaaatgtt gggaaaaacc gccttgccgc
     9481 cgaaaaagtc atagaccaca cgggcggcag agggcgcctt gacgtcgatg atgtgattgg
     9541 ccggcttgtc ggcgatgcgc ttgccttcgc tttcgtgatg atcaaaggcg acatggatgc
     9601 ccggaacgta gggcaggttg gtggaaatgt ccccggcccc gactgcgatc ttgccatcct
     9661 gcatgtcctt gggatggacg aaatcgatgc gttcaaccag gccgagttcc ttcagcaggg
     9721 tgccgcacac aaggccgtcc atgtcactgc gggtgatgag gcggaatttt tctgccggac
     9781 aatcccggcg caaagtgtcg ggcgtgatcg acttggcggt catgatggtc agggtccccg
     9841 ttggtaatgg tcgcgtgaga cgggaatgct gacgttagga cgtaaacgat ttgcccaagc
     9901 gggccgggcc ggacatcgac aagctgcgag cgtggttaat gccgccgact acagcaagcg
     9961 gtccttcggc gtgcttcaag aatttctcat gttgatcagt ataatgagaa attcttgaaa
    10021 ggatcccaat ctcgccagct ttgcagcgcg tgcggatgta aaggaccagg cgccgggtca
    10081 atcctccagt tcttcctttg cggcctcgac atcaagacat tccattgcgt ccttcggctc
    10141 gcaggaggcc gcctgctcat agagtttgag ggcgtccttc atcttcgaat tgccgaacat
    10201 catgatcagc ccggcgccga actcggtgcg ggcgacggcg gatgtcgggt tgagcttgag
    10261 cgcggtttcg aaatgcttga cgcctgcgtc cttgctggcg ccataggtca gcccgccgat
    10321 catgccgccg accttgttga tgatctcggc atggtaggta ccgagcgcga tatgtgcatc
    10381 ggcgtgtttc ggcgccaggg agatggcctg ttcgaggctg ttgcgcacct tgccggcaag
    10441 tccctcggcc agtgccttgc cgatcgagat gccctggctg tagcggccga gcgcatgggc
    10501 gtgcaaatac caggcattgg cgaggctctt ggcagcgcca atcagttcct cggcgcggct
    10561 ggcggattcc tgtaacaggg ccagcttggc cgaatccgat gcttccagat aggaggcgta
    10621 gatattggtg gccttgttgg ccacgttgta acccagaagt ccgaccgaaa gccccagttc
    10681 gacggctccg ccaaagtcgc cgcgatgata ggcacgccag gcatcctgga ccgttgcggc
    10741 agcatcatcg aacgagccct tcggcttgag gcggggatga gccttggctt gggcttccag
    10801 cgtcttggca tcgggaaacg gttcgcaatc gccctgatgc agccgcgccc aggccttggc
    10861 cagcgccgga cccttgtaat cgaagtcgga cgaagcgtgc ggaaaagctt tccagcgtgt
    10921 cttgctcatg gatcggttcc tccctcagga tttgtagcgt tgtgcgaggt ggtcaagcag
    10981 agcccgctcg tgcggggtca ggtagggtgc ggccagcgcc atgacgtgaa aggctccgcg
    11041 cgccaatgtc gtgccatcgt ccggcgcgcg cggatggcgc acataatcga atgacagcca
    11101 gtaggtggcg atcagcgtca tgttcagcgc cagtgcctcg atatcggccc ggctggcatg
    11161 catccggcca ccctggacca gtccatgcat gatggcttcg gccgtgcgtt gcttatgggc
    11221 aagaatgcgc ttgaactgta cctcgagcgg ccgatgacgg gagaccagat cggtgaggtc
    11281 gcgatagatg aaacggtatt tccagatcgc ttcgaaaacc aggtgcagga acagccagat
    11341 atcctcggtt tcggacggcc gcttttccgg ggcggcgagc atatgttcga tttcctgccg
    11401 gaacaggccg aacagcgcct cgacgatcat ttccttcgac ttgaaatggt aatagaggtt
    11461 gccggggctg atgcgcatgt cttctgcgat ggcggaggtc gtcacggccc gttcgccaaa
    11521 tcggttgaac aggtcaagcg ccgtggccag aatgcgctcc ttggtgcgcc gcggcggctt
    11581 ccgctccatg accgccgctc ctttctcccc gcgttcctgc tatcctgccc gcatcatttt
    11641 gccggcaacc gtgtcttgag cacagcattg agcgcatcga gcgtctggtc aagctccaga
    11701 gtcgcctcgc gcaggtcacg cccgtgcagg gctgaggacg ggccggaggc gatgctttgg
    11761 cggcgcgcgc cgatcacata catatccagc tcgacgccat ggcgggccag cacgggctcc
    11821 agctgttcac gccggcgcct gaggtcggcc agtgtgcgct gataggcatg ctcggcgagg
    11881 cgcttgcgac tggcgtagga aaagacattg gtgaagaaca tctccacatc gccctggccc
    11941 ggctcgaaca gcagcacatc ggcatcggga aattcggtgc gatagcggtc catgccggtt
    12001 ttcatgcgcg aatggatgat ggaccggaag gtttgcgaca tcaccgccgg cagaccgtga
    12061 tccatcagcg agaacccgcc agaaaatccc tcatcatggg cgaggctggc attgaagggc
    12121 accagcggat tgacgcaaaa caatagactt gccccctcct tgagggcgac ggaggcatgc
    12181 agcgtcttca tcagcgcccc gtcgacgtaa tagcggccgt caatctccac cggcgggaac
    12241 agacccggca gggccgttgt cgcctgcacc gcccgggcga tcggcacatg gtcccagcca
    12301 ggggcgccga acggcaccgc cttgccgctg tcgaggtcgg tcgccacaag gaacaacttg
    12361 tgcctgagtt cgcggaagtc gttggtccgt cccgggccag aaagcaggcg ggtgagaaag
    12421 gcgctgaagg cgttgttgtc gaacaacccg gtaggcaccg cacgggcgag ccgctggaag
    12481 gattcgaaga agccgtatga gagcgggttt tcaagataac gcctggccgc ctgcaaacac
    12541 agatccggga tcgcggcgag gcggcgggca tattcggcaa aagccggcct gagcaggatg
    12601 tcgggatcga aggcatgctc ggccgcctcg tcggcgatga acatgcgata catgtcgcgc
    12661 ggggtcaggc cgttggccaa gcccgtggca atcactccac cagagctgac ccctacatag
    12721 acgtcgcaat tgaccagatc gacgccgcgc agggcatcct gcagcgcaac cagcgcgccg
    12781 atctcgtaga tggcgcccag tggcccgcca ccggccagcg cgacgcctat tgccttacgg
    12841 ttgcgccgtt tcatggccac acccttaacc ttccgaacgg aaaggcatgc attcaccggc
    12901 gcagagtatc atctgcgccg gatgaacatt agctaagcga ggctagcttg tcagccgatg
    12961 gtctcggcca atcagctcag ccaacggtct tggccttgac gctggcgatc accttggcag
    13021 cctcgtcctt ggcaatcttg gccgaggtgg taaccagctc cgccgcatcg tccttgaccg
    13081 cgccagcctg cttgagcacc ttcttggccg aagtcgagac cacttcggcg gcctcgtccg
    13141 tggcagcgct ggcctgcttg agaaccttct gggccttggc cgagacgctc tgcgaggtct
    13201 tcttggcctc ggtctgcacc gtgccggaca gcttcttggt cgaagcggtc agctcatgga
    13261 cgcgctgggt cagaaggtcg atgtccttct tggtcggcac gttcaggctg tgcagggcgc
    13321 gggcaacacg ctcctcgaac acgctttcca gcttgtccca ggtgccgctg gcggccttgc
    13381 gggcttcggc aatcttgtcc tcggcgactt cgcgggtctt ggtctggatc ttctcgcctt
    13441 ccttcaccag cgcctcgaac accttcgtgc cctcttcctg cgccttggcg aaagcgccga
    13501 ggccagccag ccagatctgg tgggctgaat ccttgatcgt ctgcgcgaga tgggtctcgt
    13561 tggagccacc agcaagagcc ttgagcttct tggccatgat accattcctt cttcagagct
    13621 gctcacccgc catcaggtga catgccaaat gtgatgtggc tttcatagcg gctgcaatta
    13681 gagagtggcg cctaatcatc tagaggctgg actctaaagt tccgttgtga accaatagca
    13741 gcggccgggg aacatcggcc attcggggag ggagatccta tgcattacgt cgtgaccggc
    13801 gccagcggct tcatcggcaa gcgtctcgtg cgcgcgctgc tcgatcgacc ggaggctgtt
    13861 gtctattttg tcatgcgcaa tccgactgcg gaacgcgtcg gcgccttgcg cgcattctgg
    13921 aacgtcgatg ccaagcgcgc cgtcccgatt gaaggcgacc tcacccagcc ggtgctgggc
    13981 gtggccaaga gcaacatcaa gaagctgacc ggcaagatcg accacgtctt ccacctcgcc
    14041 gccatctacg accttgaggc cgacgccgag acggaaatgg ccgccaatgt cgatggcacc
    14101 cgcaacgcgg tgaaattcgc cgaggcggtc ggggcccgct gcttccatca tgtcagctcg
    14161 attgctgctg ccggcatgta tgacggcgtg ttccgcgagg acatgttcgt cgaggccacc
    14221 aagctcgacc atccctattt cgcctccaag cacattgccg aaaaggtggt gcgctccgaa
    14281 tgctcgatcc cctggcgggt ctatcgtccc ggcattgtcg tcggcgattc caagaccggc
    14341 gaaatggaca agatcgacgg gccctattac ttcttcaagc tcatccagaa gatgcggcgc
    14401 atgctgccgc cgtggatgcc gaccatcggc atcgagggtg gacgcatcaa catcgtgccg
    14461 gtggatttca tcgtcgcagc gctcaaccat ctgtcccatc tcaagggtca tgacggcaaa
    14521 tgcttccacc tcaccgatcc ggagccgcgc cgggtgggcg acatcctcaa cattttcgcc
    14581 cgcgccgccc atgcgccgga gatggccgcg cgcgtcaatg cctcgctgtt cagcttcgtg
    14641 cccgatgcgg tgatgaagtc gctgatggcg ctcgcccccg tgcgccgcat caagaacgcg
    14701 gtgatgaagg acctcggcct gccgccggat atgttccgct tcatcaatta cccgacgcgg
    14761 ttcgattgcc gagaggccga gaagctgctc aagccggccg gcatcaccgt gccgccaatc
    14821 gaaacatacg cctggcggct gtgggactac tgggagcgtc accttgatcc cgacctgttc
    14881 atcgaccgtt cgctgcatgg caaagtgggc ggcaaggtgg tggtgatcac cggcggttcg
    14941 gctggtatcg gcaaggccac ggccttcaag attgccggtg ccggcgggcg cacgatcatc
    15001 gttgcgcgtg atgccgaaaa gctggcggct gcggccaagg aagcggaagc gcaaggcctg
    15061 aagctgttca cctatgtcgg cgacgtctcg agccccgagc aatgcgatac cttcatcaag
    15121 aaggtgctca aggaccatgg ccatgtcgat atcctgatca acaacgccgg ccgctccatc
    15181 cgtcgcggca tcgagaactc ctacgaccgc ttccacgatt tcgagcggac aatgcagatc
    15241 aattacttcg gtgcattgcg gctgactctg ggtctgctgc cgtccatgtc ggagcgtcgc
    15301 aaggggcatg tcatcaacat ctcgtcgatc ggcgtgctga ccaatgcccc gcgcttttcg
    15361 gcctatgttg cctccaaggc ggcgctggat gcctggacgc gctgcgccgc ctccgaatat
    15421 ctcgaccgcg gcgtcgagtt caccaccatc aacatgccgc tggtgcgcac cgaaatgatc
    15481 gcgccgacca agttctatca gaatgtgccg acactctcgc ccgacgaggc ggccgatctc
    15541 atcgtcgagg cgatcatcca caagccgtcg cgggtggcga cacgcctggg cattttcggc
    15601 caggtgctgc acgcagtctc cccgcgcgtg gcgcagatca tcatgaacac ctcgttccgc
    15661 atgttcccag attcggaagc ggccaaaggc gccaagccgg gcgaggaaat gccgactgcc
    15721 gagcaggtga cgatgaccca gatcatgcgc ggcctgcacc tgtaacgaag ggtcgtcatc
    15781 ggtcacttcc ggtgaccgat ggtattacga ggccagttcg ctcgccacgg cctcaaagac
    15841 gtccggctcg atcgccagcg caaggtggcc gatcgccgga aacacgcgct cggccgtatg
    15901 cggcaggcgg ctcgaatcct gcggggcgat gatctggtcg ccggtgctcc agaccgacag
    15961 ggtcgggacc ggcggcgacc agttgcggtt gagttcggcg agccagtcgt tgtcgggctc
    16021 catttgccgc gcattgcggc caagaccgaa agtggccacc accgtgccct gatgcggtcc
    16081 gccgagtgtg atgaaacggg cgatgcggct gctgccaaac cgcctcagat aggtgcgggc
    16141 gaccagcccg cccatcgaat gggtcaccag cagcaccatg tcggcgccgg tctcctcgat
    16201 cagcgattca atgcgccggt cgagctgttg ggccatctct tcgatatcgg tgaatggcgt
    16261 ttcaaggctg atggtcgcaa cggcatggcc ccgcgcccgc aatgcgcttc ggaaccacca
    16321 ccaatagccc ctgttgcaca tgtaaccatg caccagcagg accggacgct ggccggtcgg
    16381 caggcggccg acggcatccg ggcccatgaa taagcgctcg aatggctgga tagccgagaa
    16441 aaaggccaaa aagccgcaat attcctgaaa gatggccgcg ccggtcaaag gcccgatccc
    16501 cggcggtcgc gggctggcat aaagctgcga taccacatag gcgagcgcga ggacaagccc
    16561 ctgcaagacc agaaaggcca ggacgctggc tgcggcgccc gcaacccagc cgaagccgag
    16621 atacgtataa aacagccccg tcagcgccag tgcctgtccg agcagcaaac ctctcaatag
    16681 ccaggccagc attggcttgc ctttcccgtt gtctctgact tggcgcgccc acggcactct
    16741 tggccgattg tcgcagtcga agcgcgttag ataatggctg gcagaacaat agacaatgca
    16801 gatcagccag cctccgtccg gcgggtcatc ccgatacccg ggggcctctg cactgacctg
    16861 ccgggccgcc cggggtggtg gcattgcaag ggtcttgccc gcagccgcca tccgctcctg
    16921 gtggccgggg ctccctgtga ccgatgttct tttttggatt tgtcgaccta ttggagaaac
    16981 agccgcattc tgcgttcggg ttacagtgtt gaaagggtag tattcggtcg aaaacgatcc
    17041 agccgatcga tgacaggcgg caggcaaaat tccaattccg aagtagtttt caattaactc
    17101 aaaccaccga aacctcagcc attgggccga gtgagggcat ccttcaagct tgcatcaact
    17161 ttgctcgcct atggtttggc ccgggtcaat ggcgaaagca ttggcggggt aacggaggac
    17221 acgatgccag aatcacgcgc agggacacgc cggcctgccg gtcggtttgc gcggttccgc
    17281 cgcgacgaac gcggcgcgac ggcgatcgaa ttcggcatga tctcgctgcc gttcttcgcg
    17341 atcctgtttg ccatcgccga aaccgggatt gccttcctca atggccagtt ccttgatcgg
    17401 gccgtcgaga aggccagccg caagatcttc accgggcaac tggtcaatgg ccaggcgaac
    17461 ccgcaggccc gcttccagca gttccagcag gagatctgcg ccacggtcaa cgcgttcttt
    17521 tcctgcggca atctgatgta cgatgttcag gcctacagga ccttcggcga cacgatgctg
    17581 ccgataccaa tggtcaatgg cgatctggat gcctccgcgc tgcctcgatt caaccccggc
    17641 aggtccggtg aggtggtcat tgtccgcgtc tattaccggg ttcccgtcta cgccgacctt
    17701 ctcggcgccg gaatgggcaa tcttgctggc aacaagcggc tgctggttgg cactgccgca
    17761 ttcaagaatg agccctactg atgctgtcgc tgtcctttgc gcggtctctt gccgctgtca
    17821 tgcccaaggc caaagctctg gcgcgcaacc cgctcaaccg gctggcgcgt cgccgcctca
    17881 gggcatttgg caaggccaag tcgggcatcg ccgccaccga gtttgcgctc gtcgtcccgt
    17941 tgatgctgac aatgtatttc ggcatggttg agctgacgca ggcactgggc catgctcgca
    18001 agtcggtgat catgtcccgc actctggcgg atttggtgac gcagtcggtt ggcatctccg
    18061 acaccgacat gaacaccatt ttcaccgcgg cgcgctcggt catggcacct tatcccgatg
    18121 acaggttggc catgcgcgtc acgtccgtga tgatcgacgg cgccggcacg gcctgggtgg
    18181 aatggtcgga tgtcaagaac aacaatgcaa ccaatcctta cagccagcac gcccgttgtg
    18241 ccttggccaa cagtttcatc cctgcgtccc tgcgtgtccc gcgcacttat ctggtgtggt
    18301 ccgaggtgac catccagcac attccgacga ttggccatgt ggtcgcgccg aacggattca
    18361 cgatgaccga gaagatgccg atgcgaccgc gcgtcagcac cagcatcggc cgtcagaatg
    18421 tgccgggcac ctcttgtccc gccgctgtgc cctgatcgcc tcgatcctat tgccgcgcga
    18481 aaccatctgc cacggtaagt ggctgatgac atttttaaaa gtttcattag ttttcaatag
    18541 gaaatgggaa ttcaaagcag gagccgaggg tcagttgtca tgacccttgg aatggggacg
    18601 ggggcctgtg ggagatattg cggttttcga tgtcggcaac gtgctggtgc gctgggatcc
    18661 gcgcaacctg ttccagaagg tgtttgccga tcccgagcgg atggacttct ttctcagcga
    18721 ggtttgttcg cccgaatgga accatgcgca ggatgctggg cggatgtggg ccgaggccga
    18781 agccgaagcg atcgcccggc acccggcctt cgcccgtgag gtcagactgt ttcgtggccg
    18841 ttggcatgaa atgattgacg ggccggtcga ggccaatgtc cggttgcttg aaggcctgcg
    18901 ccgccgcggt gttgcaacat tcgccatcac caatttcgca gccgatacct ttgccgaaac
    18961 gcaggagcgc tatcccttcc tgaaggcctt tgacggcatt gtctgttccg ggcttgaaaa
    19021 gatctgcaag ccagatccgg cgatctaccg gctgttgctc gatcggtatc ggctggtggc
    19081 caaggattgc gttttcatcg acgattctgc cgccaatatc gcggcagctg cggcgcttgg
    19141 tttccggacc gtgcattacg ggctggggct tgatgctgcc ggcgccttcg cccagctcgg
    19201 cctgccggtc tgatccgcgc ctatccgctt gttttcgcat ccgctttttc cgccaaccgg
    19261 tatccacttg gcgggcggat gctctactgc cgcaggacca gcgatttgcc gcggatttcg
    19321 aagctttgca ggcgtgtgaa gaaattcatg ccgacaagcg tgaccggtag ccctttgggc
    19381 acgatcaggg cctcgatgtc gcgcacgacg atgctgccga tgcggacttc gttgagtttg
    19441 acccgcgcag cctgaacaac gccgttggcg gtctgcaccg gcgaggtata ggctgatgcc
    19501 gccggcgtga tgccgagctt gcgcgcttca tctgccggga tcgcgatgac cgtcgcgcct
    19561 gtatcaagca ggcccttcag aaactggccg ttgatccgga tgtctgcttt gaaatgtccc
    19621 gattcgtctg cggcaagcgc gaccgcctgc gggccgggcg gtgccggctg cgccgccgcc
    19681 ggtgtcg
//
LOCUS       DAOMXG010000026        19649 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_1629_length_19649_cov_8.911095, whole genome shotgun sequence.
ACCESSION   DAOMXG010000026 DAOMXG010000000
VERSION     DAOMXG010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 19649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19649
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1629_length_19649_cov_8.911095"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..3260)
                     /locus_tag="PLQ11_03210"
     CDS             complement(90..3260)
                     /locus_tag="PLQ11_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003604861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRK23942.1"
                     /translation="MNVSAWSIRNPVAGILLFVVLSIIGINSFKALPITKMPNIDVPF
                     ISVVVTQGGAAPSELETQVTKKIEDAVANISGVKHITSSIGDGSSSTVIEFRLEVNTD
                     RAVNDVKDAVARIRSDLPRSIDEPVVQRVDIEGLPILVYSAYAPQMTLEEISWYVDDT
                     VIRTLQSIKGVGGVSRIGGVDREIRISLDPDRLAALGVTAGEVNGRVRATNVDLSGGR
                     SDVAGREQSIRTLAGARSLEDLAATMIPLANGRKVRLDEIGRIEDSFVEQRSFARLDG
                     KPVVAFAISRAKGASDAEIDVAVSAKIKELGARRPDVELALIDTSVRYTYGNFKSAMK
                     TLVEGSLLAVAVVFLFLRNLRATLISAVAIPLSVLPTFWAMSLAGFSLNLVSLLALTL
                     ATGVLVDDAIVEIENIERHMHMGKSPYRASLEAADEIGLAVIAISATIIAVFVPVSFM
                     SGIAGQYFKQFGLVVAVAVFFSLAVARLITPMMAAYLLRPPRHGGHGEGVQGPFTRGY
                     LTVVRSSVRHRWITLFVGALLFAACIKSADYLPKGFFSEPDTARLLLGVELAPGSRLE
                     ETIRVSDAITARIKSWPEVKSVFIDGGKVAAAGATTKEVRKATIIVNLVHKSERTKTH
                     NQLRAEISEGLREFADIRYWFMKDNGMRGVQMVVQGADGAEVSEHASRIASEMKRLPI
                     LANVVSTAALDRPEIRIRPKPGVAADLGITTDTISEAIRVATIGDVGPNLARFNAGDR
                     LVPIRVQVEEGARGKLDVIEQLKVVSSRGVAVPLASVAEVTFGQGPTSIDRYDRARRV
                     AVEADLHGTEALGEAVDAIRALPAAKALPPGLVLKEFGDAEIMGEVFSGFAQAMGLGL
                     MMVLAVLILLFGSALQPITILASLPFSVGGVILALYLTNYPISMPVVIGILMLMGIVT
                     KNAIMLVDFAVEAMARGVPQFEAIVDAGAKRARPIVMTTIAMIAGMVPSALALGDGGE
                     FRAPMAIGVIGGLIVSTVLSLVFVPAVFTIMDDLGRLLGRLLAPLIGPKDEPGEAPPA
                     ALVVPPAAEAQAAAVQAAGKALRAAE"
     gene            complement(3265..4446)
                     /locus_tag="PLQ11_03215"
     CDS             complement(3265..4446)
                     /locus_tag="PLQ11_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRK23943.1"
                     /translation="MMTRRWGWLLAALLAGHLGNGIGMAAAKDATASQTAAPAGPRVT
                     VSLAKRGPLVETLALTGTLVAREEVLVAPEIDGLQILQILAEEGDRVAAGQVLVRLRR
                     DTLEALLLQNGALLAKAEAAISQARSQIVQAEAAQTQAEQALQRVTQLRQSGFSTQAQ
                     FDQLTNDAKAALARLAAAREGLELGEADQAAIQAQRKELSIRLAATEVKAPVAGVIAR
                     RSARLGATAGMAAEPLFRIVSGGEIELEGEVSDTRIHTVQPGAPAQVTALGRVVSGTV
                     RLVSGEIDRASRLGKVRIALGADSGLKVGAFGRGVVETRRVEVITVPLPALLYSEGGT
                     VIQVVRDKRIVTRPVRTGGSAQGRVEIVEGLAEGETFVAKAGAFLRDGDAVTPVSEAE
                     K"
     gene            complement(4443..5108)
                     /locus_tag="PLQ11_03220"
     CDS             complement(4443..5108)
                     /locus_tag="PLQ11_03220"
                     /inference="COORDINATES: protein motif:HMM:NF025343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRK23944.1"
                     /translation="MPRITSLRHAERQANILRSAEVCFARSGFHRTTMQDICREAGIS
                     AGAVYVYFDSKEALIEGLVARDRDEIGEALARIGEAEDFFAALEAALQSCVLDRPAHK
                     ASLFVEIVAEAHRNQRVAQSLAGCDRQLRSMLAGLIERARAEGRLSGGLPAERLAMMM
                     AMLGDALFIRRAMDPAFDGQALVPMLMASIRALLDEPETAAPRPAAMPPRRPRVRSVA
                     GAA"
     gene            5384..6124
                     /locus_tag="PLQ11_03225"
     CDS             5384..6124
                     /locus_tag="PLQ11_03225"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRK23945.1"
                     /translation="MSSALSSPPKVAHLRPFVLSLAASSALALVLSTVMNGPGHAMSA
                     LAIGAAAPLCWLPLFGRRSASEAAPAPAPGIAEPVHMDEVTGLMTRSSFAEKAKEAFT
                     EARETGRLISAVLIQVEGLEAINETWGTPAGDAVLAHVGKLIGINIQSQGDLAARYAG
                     ASFALLLPEADHDWTTAFCERLSGCLANRPVGFGDTLIPVSARFGIAAIDRADCEVDT
                     LIRRARRQMGEVSCPVDAAPVQLDRLAA"
     gene            complement(6225..6713)
                     /locus_tag="PLQ11_03230"
     CDS             complement(6225..6713)
                     /locus_tag="PLQ11_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRK23946.1"
                     /translation="MMRSKGGGEASAGLLDLRAFIPYRLAVLAEAVSRLMGEVYSDRF
                     QLTRNEWRVLAALDSLSHATQTEVAEYSTLDKMQVSRAIAALDERGLIARSAGRADRR
                     TKLLVLTQQGRALFETIVPLVLEQQRLLLKDLAADERDALDRALDALLERARRLQTRS
                     AV"
     gene            6868..7407
                     /locus_tag="PLQ11_03235"
     CDS             6868..7407
                     /locus_tag="PLQ11_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRK23947.1"
                     /translation="MKVERFHHVAFRCKDAKETADFYNRVLNMELLGAIAEDKVPSTK
                     APDPYMHIFLDAGSGNILAFFELPNSPPMGRDENTPIWTQHIAFEVKDMAALNEAKQR
                     AEAEGIEVIGPTDHNIFQSIYFFDPSGHRLEVACWSTTPDKLKRMKDNSPAMLEEWSR
                     TRKPPKHNAWLHEKEFSKS"
     gene            7618..9246
                     /locus_tag="PLQ11_03240"
     CDS             7618..9246
                     /locus_tag="PLQ11_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRK23948.1"
                     /translation="MLKTYTYPEYPYRPSAEQREGRTLRHPVVVIGAGPIGLSAGLDF
                     ARRGIPVVILDDNNTVSVGSRAVCYAKRPLEILDRLGCADPLVAKGISWKIGKVFVRD
                     RLAYQFDLLPESDHKMPAMINLQQYYLEEAMIAAIGREKNADLRWKHKLIALKQHDDH
                     ATLTIETPDGHFTMEADWVVACDGANSDTRRMTGGDFVGQFFQDRFLIADVVMQADFP
                     TERWFWFDPPFHPNQSVLLHKQSDNVWRIDFQLGWNADPNEEKKPEKVIPRIRAMLGP
                     NVPFELEWVSVYQFACRRMQSFRSGRVLFAGDAAHQISPFGARGANTGFQDIDNLGWK
                     LKLVLDKVAPEALLDSYDAERIFAADDNLLNSTRSTDFITPKSVMSRRFRDAVLELAE
                     TVEGIRPLVNSGRLSRPTPYLDSPLNTPDTAPFKGRMAPGTNCDDAPVTIAGKPGWLL
                     NLLGEGFTVLTFDTRPPVAKLDAGPLSARVLTVGQDFADTRGLVAARYDGEIGTTYLI
                     RPDQHVAARWRSFDAAKITAAMRRACAIPAAGRR"
     gene            9277..9489
                     /locus_tag="PLQ11_03245"
     CDS             9277..9489
                     /locus_tag="PLQ11_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2783 domain-containing protein"
                     /protein_id="HRK23949.1"
                     /translation="MKLTTRANINDPDGFYQELIEAQRDLGDEEAARMTCKLVILLAN
                     HIGDRAVLSEALRLAAPKSAAIPPKA"
     gene            complement(9545..11830)
                     /locus_tag="PLQ11_03250"
     CDS             complement(9545..11830)
                     /locus_tag="PLQ11_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012452684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HRK23950.1"
                     /translation="MSKDLYSGALIYHKLPKPGKLEVVPTKPLGNQRDLALAYSPGVA
                     AACEEIRKNPETAADYTARGNLVAVISNGTAVLGLGDIGPLASKPVMEGKAVLFKKFA
                     GIDVFDIEVSEKTVDKLVEVVASLEPTFGGINLEDIKAPECFEVERLCRERMNIPVFH
                     DDQHGTAIIVAAAVRNGLSLVGKDIAKVKIVASGAGAAALACLNLLVSLGASIGNIWI
                     SDIEGVVYEGRKELMDQYKSKFAKKTKARTLAEIIDGADVFLGLSAGGVLKPEMLKSM
                     AKDPLILALANPSPEIMPEDALAARPDAMICTGRSDYPNQVNNVLCFPYIFRGALDCG
                     ATAINEEMKLAAVEAIAGLAREAPSDVVARAYGGEARLFGPTSLIPSPFDPRLILRIA
                     PAVAEAAARTGVALRPIEDLKAYADSLQRFVFRSGFVMKPVVAAAKADPKRVIYADGE
                     DERVLRAVQVVLEDGLAVPILIGRPAVVEARLKKFGLAIRPGKDFQLVNQEDDPRYRD
                     YVQTYLDRAGRKGITPEAARVLVRTSTTVVAALAVARGEADAMICGLEGRYRSKLRQI
                     EEIIGLAPGRRDFAAMSLIITSKGHYFLTDTHVQPDPTAEELAEITIAAAEHVQRFGI
                     APKIALVSHSDFGAHDTPSAKKLRRTLDLVRMKAPKLEIDGEMEADSALLAEVRDRVL
                     PSSTLKGPANVLVFPNLDAANVAYQFAKVLANGQPVGPILLGPAKPAHVLTNSVTSRG
                     VVNITAIAVAEAQALAGESRL"
     gene            complement(12087..12899)
                     /locus_tag="PLQ11_03255"
     CDS             complement(12087..12899)
                     /locus_tag="PLQ11_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HRK23951.1"
                     /translation="MPNVSLSHSVRQNLTALQQTADMMSVSQSRLASGKKVNSALDNA
                     TNFFTSQELNGRAGDLQGLLDGISNATKALEAANNAITAITKLVESAQATVRSAQSNP
                     SNAAAMVTQFNSIRDQITDLADDASFNGVNLLKSTTTMKVAFNELTGAAKNELSVAGV
                     DLSATGLAITAATALDDTEATAKLGLLTTALGTLRSTAATFGANLSIIQARKDFTKSS
                     ITTLKSGADNLVNADTNEEAATLLALQTRQQLSQSALSMANQQDQAVLRLFS"
     gene            complement(13000..13308)
                     /locus_tag="PLQ11_03260"
     CDS             complement(13000..13308)
                     /locus_tag="PLQ11_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23952.1"
                     /translation="MNDSSKARAIGVIAAQIEAAASLLRFAADDLAEIIQSNSTIFQN
                     EFDLIIDNLRNTSIGIEGHGAFLLRSDLFELADTNSGQSDYDAPAPVNALQNRRHHRL
                     "
     gene            13548..14516
                     /locus_tag="PLQ11_03265"
     CDS             13548..14516
                     /locus_tag="PLQ11_03265"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HRK23953.1"
                     /translation="MRKPIWIDTDVGFDDMAALAMVHSDPNWAVLGLGLSYGNARLDQ
                     AVAQAQRMRGFFGWRTPLHAGSAKPILGPAMTGEALFGPTGLRTIGRTLPDCESEIAR
                     EDAVTGMTRALAGADAPVTVLALGPLTNIAIALLARPELARLIERIVWMGGAAIGGNH
                     TATTEFNAACDPEALAIVLESGVAFRMIGLECCRQVTITGTDAAKLRALQTERARILA
                     DLMDGYVRLASVDGSRPMPVYDAVAAAAVLDDRAVAFIAANISVERAGALTRGTTICE
                     FRPQKAAPNAEIARTALATRVFDRLLADFTAAAVADPASDTISRAS"
     gene            complement(14552..15724)
                     /locus_tag="PLQ11_03270"
     CDS             complement(14552..15724)
                     /locus_tag="PLQ11_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019905273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HRK23954.1"
                     /translation="MSVIPRLAEWAGAFAAIRQDLHAHPELGFEEHRTSAIVAEKLES
                     WGIEVHRAIARTGVVGVLRGRGASNRAIGLRADMDALAMQEADTLAYRSTVPGKMHGC
                     GHDGHTTMLLAAARYLAETRNFAGTVHFIFQPAEEGGGGARVMLEEGLFDRFPCDSVY
                     AAHTDPLLPLGVITAVAGPVMASADHFDIRIHGKGGHAARPHHAIDPVLVGSHIVVAL
                     QSIVSRRTNSFDSAVVGVSTFNAGSAMNVIPEKALLTGTVRTLRAETQHEIQRLLTHM
                     VMTTAEAHGAAADVDYHRGYPPVVNSVHEADCAARAAASVVGADKVLTYHSPSMGGED
                     FSYMLLQRPGCYVKLGQAADARSAIALHNQSFDFNDSLTPIGASYFARLVEQELPL"
     gene            complement(16050..17204)
                     /locus_tag="PLQ11_03275"
     CDS             complement(16050..17204)
                     /locus_tag="PLQ11_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRK23955.1"
                     /translation="MSFTSNWNYPTPIKFGPGRILELAQHCLANNIRRPLLVTDAGLA
                     KLPMVAEAVAANAAAGVPTAVFADVKPNPVRANVEAGARQFREGGHDGVIAFGGGSAL
                     DAGKAIAFYAHQSRPLWDFEDVGDNFLRADVAKIMPSIAVPTTSGTGSEVGRVSVITD
                     EETHTKRLIFHPKIMPVTVVADPALTIGVPPAITAATGMDALAHCLEAYCAPGFHPMA
                     DGIALEGMRLIKDWLPVATRDGRSIEARAQMMVAATMGAAAFQKGLGVIHSLAHPLGA
                     IYDSHHGLLNAILMPYALDFNRAVLGDKMQRLAAYLGLDRQDMDGVIDWVLDLRTALD
                     IPDTLGAIGIDDGKADTIVAAALNDPTAPTNPVALEQGPVRELLLRAITG"
     gene            complement(17221..18609)
                     /locus_tag="PLQ11_03280"
     CDS             complement(17221..18609)
                     /locus_tag="PLQ11_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006339002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRK23956.1"
                     /translation="MQIQKTISPVDGRIYVERPLASSAEIAWALNAAVKAQAEWAGVP
                     LDQRAAALSRFVDAFVAKRDAIAEELTWQMGRPIRYTPGEVRGFEERARTMIALAPEA
                     LADIAVGEKAGFRRFIRRAPLGVVLVVAPWNYPYLTAVNAVVPALMAGNCVLLKHAAQ
                     TPLCAERFAEAAREAGLPKGLLQALHMSHEAVAALIGMPEIAHVCFTGSVPGGEAMER
                     AAAGRFIGVGLELGGKDPGYVRADADIAHAVENLVDGAFFNSGQSCCGIERIYVHEKL
                     YDEFVERAVMLTRSYLLDDPTRPETTLGPMVRTAAADLVRGHIDEAVAHGAIALLSEK
                     SFPRSQAGTPYLAPQILVEVDHTMKVMREESFGPVVGIMKVAGDEEALKLMNDSAFGL
                     TAAIWTSDAEAAIALGDRLQTGTVFMNRCDYLDPELAWTGVKHSGRGATLSKVGYEHL
                     TRPKSFHLRTTL"
     gene            complement(18618..>19649)
                     /locus_tag="PLQ11_03285"
     CDS             complement(18618..>19649)
                     /locus_tag="PLQ11_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020180377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HRK23957.1"
                     /translation="EPICPRGTLRRVLARAADMGFAASAALEYEFFLFNETPESVRAK
                     GYRDLKPITPGFFGYSVLRSSVLSELYREMLDTFRAMDCEIEGLHTETGPGVLEAAIA
                     VDDALRSADKAVLFKTFAKVFAQRRGMMATFMAKWSRDWPGQSGHIHLSLKDKKSGKP
                     VFHEAGKPYSMSDTMRWFLGGQQKLLPEILGMISSTVNSYSRLIPGYWAPTSATWGVE
                     NRTTALRVVPGSPKSQRIECRIAAADANPYLALAAALGAGLWGIEHRIEPTPIVIGNA
                     YDKKHPKNLTIPATLWEAAQRLKASRPARALFGDAFVDHFAATREWEEREFRKHITDW
                     ELARYFEII"
BASE COUNT         3620 a         6648 c         5929 g         3452 t
ORIGIN      
        1 cgacgccgag cagggccgaa ggcccggttg cgagggacgg cccgggaaat gaccgacagg
       61 tcatttctga gggccactgg tataacccgc tactccgctg ccctgagcgc cttgccggcc
      121 gcctgaactg cggctgcctg ggcctcggca gccggcggca ccaccagagc cgctggcggc
      181 gcctcgcccg gttcgtcctt gggcccgatc agcggagcaa gcagccgccc gagcagccgg
      241 cccagatcat ccatgatcgt gaataccgcc ggcacgaaca ccaggctgag cacggtggag
      301 acgatcagcc cgccgatgac cccgatcgcc atcggcgcgc ggaattcccc gccatcgccg
      361 agcgccagcg ccgagggcac catgccggcg atcatggcga tggtggtcat cacgatcggc
      421 cgggcgcgct tggcgccggc gtcgacgatc gcctcgaact ggggcacgcc gcgcgccatc
      481 gcctcgacgg cgaaatcgac cagcatgatc gcgttcttgg tgacgatccc catcagcatc
      541 aggatgccga tcaccaccgg catcgagatc ggatagttgg tcaggtagag cgcgagaatg
      601 acgccgccga ccgagaacgg cagcgaggcg aggatggtga tcggttgcag cgcgctgccg
      661 aacagcagga tcagcaccgc cagcaccatc atcagcccca gccccatcgc ctgggcaaat
      721 ccggagaaca cctcgcccat gatttcagcg tcgccgaatt ccttgagcac cagccccggc
      781 ggcagcgcct tggccgccgg cagcgcgcgg atcgcatcca ccgcctcgcc aagtgcctcg
      841 gtgccgtgaa gatcggcctc caccgcgacg cgccgggcgc ggtcatagcg atcgatgctg
      901 gttggcccct ggccgaatgt cacctcggcc actgaggcca gcggcacggc taccccgcgc
      961 gaggaaacca ctttcagttg ctcgatgacg tcaagcttgc cgcgcgcgcc ctcctcgacc
     1021 tgaaccctta tcggcaccag ccggtcgccg gcgttgaacc gggcgaggtt tggcccgaca
     1081 tcgccgatcg ttgccacccg gatcgcttcg gaaatcgtat ccgtggtgat gccgagatca
     1141 gcggcaacgc cgggcttggg gcgaatgcgg atctccggcc ggtcaagcgc tgcggtcgag
     1201 acgacattgg caaggatcgg cagccgcttc atctcggagg cgatgcgcga ggcatgttcg
     1261 gaaacctccg cgccatcggc cccctgcacg accatctgca cgccgcgcat gccgttgtcc
     1321 ttcatgaacc agtagcggat gtcggcaaac tccctcagcc cctcgctgat ctcggccctg
     1381 agctggttat gcgtcttggt ccgctcggac ttgtggacca gattgacgat gatggtggcc
     1441 ttgcgcacct ccttggtggt cgccccggcg gcggcaacct tgccaccgtc gatgaacacg
     1501 cttttcacct ccggccacga tttgatcctg gcggtgatcg catcgctgac ccggatcgtc
     1561 tcctccagcc gcgatccggg cgccagttcg acgccgagca gcagccgcgc cgtatccggc
     1621 tccgagaaaa accccttagg cagataatcg gccgacttga tgcaggcggc aaacagcagg
     1681 gcaccgacaa acagcgtgat ccagcgatgc cgcaccgagc tgcgcacaac cgtcaggtaa
     1741 ccgcgcgtga acggcccttg cacaccctcg ccgtgaccgc catgacgcgg aggccgcagc
     1801 agataggcgg ccatcatcgg ggtgatcagc cgcgccacag ccagcgagaa gaacaccgcc
     1861 accgccacca ccagcccgaa ctgcttgaaa tactgcccgg caatgccgct catgaacgac
     1921 acaggcacaa agaccgcgat gatggtcgcc gaaatggcga tcaccgccag gccgatctcg
     1981 tcggcggcct ccagcgacgc gcgatagggc gatttgccca tatgcatgtg ccgctcgata
     2041 ttctcgatct cgacgatcgc atcatcgacc agaacgccgg tcgccaatgt cagcgccagc
     2101 agcgagacca gattgagcga aaagccggcc agactcatcg cccagaaggt cggcagcacc
     2161 gacagcggaa tggccactgc cgaaatcaac gtggcccgca gattgcgcag gaacaggaaa
     2221 accaccgcca cggccagcag cgatccttcc accagcgttt tcatcgccga tttgaaattg
     2281 ccgtaggtgt agcgcaccga agtatcgatc agcgccagtt cgacatccgg gcggcgcgcg
     2341 ccgagttcct tgatcttggc cgagaccgcc acatcaatct cggcatccga agcacccttg
     2401 gcgcgcgaga tggcgaaagc gaccaccggc ttgccgtcga gccgcgcaaa actgcgctgt
     2461 tcgacaaacg aatcctcgat gcggccgatc tcgtcgaggc gcaccttgcg gccattggcc
     2521 agcggaatca tcgtggccgc caggtcctca aggctgcgcg ctcccgccag cgtgcggatc
     2581 gactgttcgc gaccggcgac atcgctgcgc ccgccggaca gatcgacatt ggtggcgcgc
     2641 acgcggccgt tgacctcgcc ggccgtcacg ccgagcgccg ccagccggtc cggatcgagg
     2701 ctgatgcgga tttcgcgatc gacgccgccg atccgcgaca cgccgccaac acccttgatg
     2761 ctctgcaagg tgcggatcac cgtatcgtcg acgtaccagg agatttcctc cagcgtcatc
     2821 tgcggcgcat aggcgctgta gacgaggatc ggcaatccct cgatatcgac ccgctggacc
     2881 accggctcgt cgatcgaccg cggcaggtcg gagcggatgc gcgccacggc atccttgacg
     2941 tcgttgaccg cccggtcggt gttcacctcc agccggaatt cgatcaccgt ggacgacgaa
     3001 ccgtcgccga tcgacgaggt gatgtgcttg acgccggaaa tgttggcgac cgcatcctcg
     3061 atcttcttgg tcacctgcgt ttcaagttcc gacggcgccg ccccgccctg cgtcaccacc
     3121 acagagatga acggcacgtc gatgttcggc atcttggtga tcggcagcgc cttgaagctg
     3181 ttgatgccga tgatcgacag caccacgaac agcaggatgc cggcgaccgg attgcggatg
     3241 gaccaggcgg aaacgttcat ggcctcactt ctccgcttcg ctcaccggcg tcaccgcatc
     3301 gccatcgcgc aggaaagccc cggccttggc cacgaaggtc tcgccctcgg ccagaccctc
     3361 gacgatctcg actcggcctt gggcgctgcc gccggtcctg accgggcggg tgacaatccg
     3421 tttgtcgcgc accacctgga tgacagttcc gccctcgctg tagagcagcg ccggcagggg
     3481 caccgtgatc acctccaccc gccgcgtctc gaccacacca cgcccaaagg cacccacctt
     3541 aaggccgctg tcagcgccaa gcgcgatgcg caccttgccc aatcgcgaag cgcgatcgat
     3601 ctcgcccgaa accagccgga cagtgccgct gaccacccgc cccaacgccg tcacctgcgc
     3661 cggcgcaccc ggctggacgg tgtggatgcg cgtgtcggac acctcgccct caagctcgat
     3721 ctcgccgccc gatacgatcc ggaacaacgg ctctgccgcc atgcccgccg tcgcgccgag
     3781 gcgtgcgctg cgccgggcga tcaccccggc caccggcgcc ttcacttccg tcgccgccag
     3841 ccggatcgac aattccttgc gctgagcctg aatggccgcc tgatcggctt cgcccagttc
     3901 cagcccttcc ctggccgccg ccagccgcgc caacgccgcc ttggcatcat tggtcagctg
     3961 gtcaaactgc gcctgggtgg aaaaacccga ctgccgcagc tgcgtgaccc tctgcaacgc
     4021 ctgctcggcc tgcgtctggg ctgcttcggc ctgcacaatc tggctgcggg cctgactgat
     4081 cgccgcctcc gccttggcca gaagcgcgcc attctgcagc agcaaagcct ccagcgtatc
     4141 gcgcctcagg cggacgagaa cctgcccggc ggcaacccga tcaccctctt cggcaagaat
     4201 ctgcaaaatc tgcaacccgt cgatttccgg cgcaaccagc acttcctcgc gcgccaccag
     4261 cgtcccggtg agggccagcg tctccaccag aggcccgcgc tttgccagac tcaccgtcac
     4321 acggggaccg gccggcgccg cagtttgcga cgccgtcgca tccttggctg cggccatgcc
     4381 gattccattg cccaaatgcc ctgccagcag cgccgccagc agccatcccc atcgcctggt
     4441 catcatgcgg cccccgcgac agaacgcacc cgcgggcgcc gcggcggcat cgccgccggc
     4501 cgtggcgcag ccgtctccgg ttcgtccagc agggcgcgga tcgacgccat cagcatcggc
     4561 accagcgcct gcccgtcaaa tgccggatcc atggcccgcc ggatgaacag ggcatcgccc
     4621 agcatcgcca tcatcatcgc cagccgttct gccggcagtc cgccggacag gcgtccttcg
     4681 gcgcgcgccc gctcgatcag tcccgccaac atcgaacgca gctggcggtc gcaaccggcc
     4741 agcgactgcg ccacgcgctg gttgcgatgg gcttcggcga cgatttcgac gaacaggctc
     4801 gccttgtgcg ccggccggtc aagcacgcag ctttgcagcg ccgcctccag cgcggcgaag
     4861 aaatcctccg cctcgccgat ccttgccagc gcctcgccga tctcgtcgcg atcgcgggcc
     4921 accagcccct cgatcagcgc ctccttcgaa tcaaaataga catagaccgc cccggcgctg
     4981 atgccggcct cgcggcagat gtcctgcatg gtggtgcggt gaaatcccga acgggcgaag
     5041 cagacctccg ccgatctgag aatattggcc tgcctctcgg catgcctgag cgaggtaatt
     5101 cgtggcatgg atctcaaaac gaatgaacgt tcgtttttta tgtaggtatc gacaggcaac
     5161 ctgtcaatcc gtcatgggca agccggttac gaacccgtca accccgtccc tgcactgcgg
     5221 cagactgtta accaaggcac gaaatccctg accctgcgtc atccgttcag ccattggaca
     5281 gggggtttgg ctaaaacttg aatgaatgct gcaacgcggc aaggacgttc gtttgctaat
     5341 caccctggca cggttcagtc atccattcac ggaagcttcc cacgtgtcct ccgcattgtc
     5401 gtcgccaccc aaggtggccc acctgcgccc gtttgtcctg tcactggcgg cttcctcggc
     5461 gctggcactg gtgctgtcca ccgtgatgaa tggccccggc catgcgatga gcgctctggc
     5521 catcggcgcc gctgcgcctc tctgctggct gccgctgttc ggacgccgct cggcatccga
     5581 agccgcccct gctccggcgc cagggattgc cgagccggtt cacatggacg aggtgaccgg
     5641 cctgatgacc cgcagcagct ttgccgaaaa ggcgaaggag gcttttaccg aagcgcgaga
     5701 gaccggacgg ctcatctctg ccgttctcat tcaggtcgag gggttggaag cgatcaacga
     5761 aacctgggga accccggccg gtgatgccgt cctcgcccat gtcggcaagc tgatcggcat
     5821 caacatccag tcacagggcg atctcgccgc ccgctacgcc ggcgcctcgt ttgcgcttct
     5881 gctgccggaa gccgatcacg actggacgac agccttctgc gagcggctca gcggctgcct
     5941 tgccaatcgc ccggtcggct ttggtgatac gctcattccg gtctcggcgc gctttggcat
     6001 tgcggcgatc gacagggccg actgcgaagt cgacacgctg atccgccggg cccgccgaca
     6061 gatgggcgaa gtctcctgtc cggttgacgc cgcccctgtt cagctggacc ggctcgcagc
     6121 ctaaagcatt ttcgagcgaa gtggagtccg gttcgcgtta agaaaatgcg agaaaacaga
     6181 aagataggac attcttacgt gaattggaat tcgctggagg cgctctaaac cgccgaccgc
     6241 gtctgcaatc tccgggcccg ttcgagcagg gcgtccagag cacggtcgag cgcatcgcgc
     6301 tcatcggcgg caagatcctt cagcaacagc cgctgctgtt ccagcaccag cggcacaatc
     6361 gtctcgaaca gggcccgccc ctgctgggtc agcaccaata gcttggtgcg cctgtccgcc
     6421 cgcccggcgc tgcgcgcgat gagaccccgc tcgtccagcg cagcgatggc ccggctgacc
     6481 tgcatcttgt ccagcgtcga atactcggca acttcggtct gggtcgcatg cgacaggctg
     6541 tccagcgccg ccagcacgcg ccattcgttg cgggtaagct ggaaccggtc cgaatagacc
     6601 tcgcccatca gccgcgacac ggcctcggcc agaaccgcca gccgataggg aatgaaagcc
     6661 ctgagatcga gcagaccagc gcttgcctct cccccgcctt tgctccgcat catgccgcct
     6721 cccgaccaat cactaacatc cgttaccaat gaatggcaac ccatgcccaa gaaaacgggc
     6781 tgtggaacgg gcaggctgat gcaaaaacgg tcttgaccga tttggtaaca ttagttacta
     6841 aatgcggatc atatcaggga gggtcgtatg aaggtcgaac ggttccacca cgtcgcgttc
     6901 cgctgcaagg acgctaaaga gacggcggat ttctacaatc gggttctgaa catggaactg
     6961 ctcggcgcca tcgccgagga caaggtgccc tccaccaagg cgccggatcc ctacatgcat
     7021 attttccttg atgccggctc cggcaacatc ctggcgttct ttgaactgcc caattccccg
     7081 ccgatgggcc gcgacgagaa caccccgatc tggacccagc acatcgcttt cgaggtcaag
     7141 gacatggccg cgctcaacga ggccaagcag cgcgccgagg ccgagggcat cgaggtgatc
     7201 ggcccgaccg accacaacat cttccagtcg atctatttct tcgatccgtc cggccaccgg
     7261 ctggaagtgg cctgctggtc gacgacgccc gacaagctaa agcgcatgaa ggacaattcc
     7321 ccggcgatgc tggaggaatg gtcacgcacc cgcaagccgc ccaagcacaa tgcctggctg
     7381 cacgagaagg aattctccaa gagctgagcc cccgctcctc accggccgga ttgaggcata
     7441 aaaccaagtc gcaaggcgag gaactcgccg cgacttggtt cgtttcaaaa atttctcact
     7501 tttatcaacg agatgagaaa tttttgagtt gcaccaaaag ccactttcaa tgacttttgg
     7561 cataagccgc gcctcaatcc ggtcgtcccg ccatgcagcg atcctcaagg catcgtcatg
     7621 ctcaagacct acacctatcc cgaatatccc taccgccctt ctgccgagca gcgcgaaggc
     7681 cgaaccctcc gccatcccgt cgtggtgatc ggcgccggtc cgatcggcct gtcggccggg
     7741 ctcgattttg cccgccgcgg catcccggtg gtcatcctcg acgacaacaa caccgtgtcg
     7801 gtcggctcgc gcgccgtctg ctacgccaag cggccgctgg aaatccttga ccggctcggc
     7861 tgtgccgatc ctctggtggc caagggcata tcctggaaga tcggcaaggt ctttgtgcgt
     7921 gatcgactgg cctaccagtt cgatcttctg cctgagagcg atcacaagat gccggcgatg
     7981 atcaacctcc agcagtatta tctggaggag gcgatgattg ccgccatcgg ccgggaaaaa
     8041 aacgccgatc tgcgctggaa gcacaagctg atcgccctca agcagcatga tgaccacgcc
     8101 accctgacca tcgagacgcc ggacggccac ttcaccatgg aggctgactg ggtggtcgcc
     8161 tgcgacggtg ccaattccga cacccgccgc atgaccggtg gcgactttgt cggccagttc
     8221 tttcaggatc gcttcctgat cgccgatgtg gtgatgcagg ccgatttccc caccgagcgg
     8281 tggttctggt tcgacccgcc gtttcacccc aaccagtcgg tgctgctgca caagcaatcc
     8341 gacaatgtct ggcgcattga tttccagctt ggctggaatg ccgaccccaa cgaagagaag
     8401 aagcccgaga aagtcatccc ccgcatcagg gcgatgctgg gaccgaatgt gccgttcgaa
     8461 ctggaatggg tttcggtcta tcagtttgcc tgccggcgga tgcagagctt ccgcagcgga
     8521 cgggtgctgt ttgccggcga tgcggcccat cagatctccc cgttcggcgc gcgcggcgcc
     8581 aacaccggct ttcaggacat cgacaacctt ggctggaaac tcaagctggt gctcgacaag
     8641 gtggctcccg aggcgctgct cgatagctac gatgccgagc gcatcttcgc tgcggacgac
     8701 aacctgctca actcgacccg ttcaaccgat ttcatcacgc ccaagagcgt gatgagccgc
     8761 cgtttccgcg atgcggtgct ggaactggcc gagacggtgg aaggcatccg cccgctggtc
     8821 aattccggcc ggctgtcgcg gccgacaccc tatctcgatt ctccgctcaa cacccccgac
     8881 acagcgccgt tcaaagggcg catggctccg ggcacgaatt gcgatgatgc cccggtgacc
     8941 atcgccggca agcccggctg gcttctcaat ctgctcggcg agggcttcac cgtgctgacc
     9001 ttcgacacaa ggccaccggt tgccaagctc gatgccggcc cgctcagtgc ccgggttctg
     9061 acagtcggcc aggattttgc cgacaccagg ggactggtgg ccgcgcgcta cgatggcgag
     9121 atcggcacca cctatctgat caggccggat cagcatgtgg ccgcgcgctg gcgcagcttt
     9181 gatgcagcaa agatcaccgc cgccatgcgg cgcgcctgcg ccatccctgc ggccggccgg
     9241 cgttgaacgg caggcgacga gaagcggaag acagccatga aactgacgac cagagccaac
     9301 atcaacgacc ctgacggctt ctatcaggag ctgatcgagg cccagcgcga cctcggcgac
     9361 gaggaggccg cccgcatgac ctgcaagctg gtgatcctgc ttgccaacca catcggcgac
     9421 cgcgcagtgc tgagcgaagc cctgcgcctc gccgctccca aatccgctgc aatcccgccg
     9481 aaggcgtagg agcaaagcga cgccgagctg atgcgaggcc tcccttgccg caggcaaggg
     9541 ccactcaaag acgcgactct cccgccaggg cctgcgcttc ggcaaccgcg atcgcggtga
     9601 tgttgacgac gccgcgcgag gtgaccgaat tggtcagcac atgcgccggc ttggccggcc
     9661 ccagcaggat cggccccaca ggctggccat tggccagcac cttggcgaac tggtaggcaa
     9721 cgttcgccgc atcgagattg ggaaacacca gcacattcgc cggccccttc agtgtcgatg
     9781 acggcagcac gcgatcgcgc acctcggcca ggagggcgct gtcggcctcc atctcgccgt
     9841 cgatctcaag ctttggcgcc ttcatccgca ccagatccag cgtccgccgc agcttcttgg
     9901 ccgagggcgt gtcatgcgcg ccgaagtccg aatgcgacac cagcgcgatc ttcggcgcga
     9961 tgccgaaacg ctgcacatgt tcggcggcgg cgattgtgat ctccgccagt tcctcggcgg
    10021 tcggatctgg ctgcacatgc gtatcggtga ggaaatagtg cccctttgat gtaatgatca
    10081 ggctcatggc ggcaaaatca cgccggccgg gcgccagacc gatgatttcc tcgatctgcc
    10141 gcagcttgga acggtagcgg ccctcaagcc cgcagatcat cgcatccgcc tccccgcgcg
    10201 ccaccgccag cgccgccacg accgtggtcg aggtacgcac cagcacgcgc gcggcttccg
    10261 gggtgatgcc cttgcgaccg gcgcggtcga gataggtctg cacataatcg cgatagcgcg
    10321 ggtcatcctc ctggttgacc agctggaaat ccttgcccgg ccggatcgcc aggccgaatt
    10381 tcttcagccg cgcctcgacc acagccgggc ggccgatcag gatcggcacc gccagcccgt
    10441 cctccagaac cacctgcacc gcccgcagca cacgttcatc ctcgccatcg gcatagatga
    10501 cccgcttggg gtcggccttg gcggcggcga ccaccggctt catcacgaag cccgagcgga
    10561 acacgaaccg ctgcaggctg tcggcatagg ccttgaggtc ctcgatcggc cgcagcgcga
    10621 caccggtcct tgccgccgcc tcggccacag ccggtgcaat ccgcagaatc aaccgcggat
    10681 cgaagggaga aggaatgagg ctggtcggcc cgaacagccg cgcctccccg ccataggcgc
    10741 gcgccaccac atcggacggc gcctcgcgcg ccaggcccgc aatggcctcc accgctgcca
    10801 gcttcatctc ctcgttgatt gccgtcgctc cgcaatcgag ggcgccgcgg aagatatagg
    10861 gaaagcacag gacgttgttg acctggttgg gataatccga ccggccggtg cagatcatcg
    10921 catccggccg cgccgccagc gcatcttccg gcatgatctc cggcgaggga ttggccagcg
    10981 cgagaatcag cggatccttc gccatgctct tcagcatctc gggcttcagc acaccacccg
    11041 ccgacagacc gagaaacaca tccgccccgt cgatgatctc ggccagcgtg cgtgccttgg
    11101 tctttttggc gaacttggac ttgtattggt ccatcagctc cttgcggcct tcgtagacga
    11161 caccctcgat atccgagatc cagatattgc cgatgctggc gccgagcgag accagaaggt
    11221 tgagacaggc aagcgccgcc gcacccgccc cggaggcgac gatcttgacc ttggcgatgt
    11281 ccttgccaac cagcgacaac ccgttgcgca ccgccgccgc cacgatgatc gccgtgccgt
    11341 gctgatcgtc gtgaaacacc ggaatgttca tgcgttcgcg gcacagacgt tccacctcga
    11401 aacattccgg cgccttgatg tcctcgaggt tgatgccgcc gaatgtcggc tccagcgagg
    11461 ccacgacttc gaccagctta tcgacggtct tttcgctgac ctcgatatcg aacacgtcga
    11521 tgccggcaaa cttcttgaac aggactgcct tgccttccat caccggcttg gaggcgagcg
    11581 gtccgatgtc tccgaggccg agcacggccg ttccgttcga gatcaccgca accagattgc
    11641 cccgcgccgt ataatcggcc gccgtctcgg gattcttgcg gatttcttcg caggccgccg
    11701 cgacgccggg agaataggcc agcgccaggt cgcgctggtt gccgagcggc ttggtcggca
    11761 ccacctcgag tttacccggc ttgggcagct tgtggtaaat cagcgcgccg gaatagagat
    11821 ccttgctcac aggccgcgtc ctccgcttgg gtttgtcttt gaaaaaaccc taaaggcttt
    11881 ggcggacttt gcaaattgca tcgcagtcaa gcattccatg cacaaaaagc atgactagat
    11941 gcgaacccat gcaccacacg ataaaagggc ggcacagcgg ccgcccccag gagccggcga
    12001 ccagcgccgc cggaaaaact gcgcaacttg ccggcgcatt gggcaggctg cggcttccgc
    12061 cgcagccgcc aggccggagc agcgcgttac gagaacagcc gcagcaccgc ctgatcctgc
    12121 tggttggcca tcgacagcgc cgactgcgac agctgctgcc gggtctgcaa tgccagcagc
    12181 gtagccgctt cctcgttggt atcggcgttg acaagattgt cggcgcccga cttcagcgtg
    12241 gtgatcgacg actttgtgaa gtccttgcgg gcctgaatga tcgacaggtt ggcgccgaag
    12301 gtcgcagcgg tggaacgcag cgtgccgagt gccgtggtca gcaggccgag cttggccgtc
    12361 gcctcggtgt cgtcaagcgc cgtcgcagcg gtgatcgcca gaccggtcgc ggacagatcg
    12421 acaccggcga ccgacagttc attcttggcc gcgccggtca attcgttgaa cgccaccttc
    12481 atcgtcgtcg tcgacttcag gaggttgacg ccattgaagc tggcatcatc agccagatcg
    12541 gtgatctggt cacggatgga attgaactgg gtgaccattg ccgcggcatt gctcgggttc
    12601 gactgggccg aacgcaccgt cgcctgcgcc gattccacca gcttggtgat cgcggtgatg
    12661 gcgttgttgg ccgcttcaag cgccttggtg gcgttggaga tgccatcaag caacccctgc
    12721 agatcgccgg cgcgaccgtt caactcctgc gacgtgaaga aattcgtggc gttgtccagc
    12781 gcggaattga ccttcttgcc ggaggccaga cgcgactgtg atacggacat catgtccgca
    12841 gtctgctgca gcgccgtcag gttctggcgc acggaatggg aaagcgaaac attcggcatg
    12901 gatgcgacct cgcaaaaaca atctcagcgg gccattcccg catatcgcaa ccctgacgcg
    12961 aaattatgaa ttgaacgtaa ggatggccgc cgacttatat cacaacctgt gatggcggcg
    13021 attctgcagt gcatttaccg gggccggagc atcataatcg ctctgtccac tattggtatc
    13081 tgccaattca aaaagatctg atctaagcaa aaatgctccg tgtccttcaa ttccgatact
    13141 tgtattgcgc aaattatcga taattaggtc aaattcattt tgaaatattg ttgagtttga
    13201 ttgtattatt tctgccagat catccgctgc aaacctcaaa aggcttgccg ctgcttcaat
    13261 ctgcgcagcg ataacaccaa tggcacgcgc ttttgacgaa tcattcataa cgattctctt
    13321 ttgcttgcta ccgggtttgg atcctactac gtgacgcaag gacaaccaat acaattttcg
    13381 gttgcactct gacgcttgtt gccacagggg cgcgcagaac gcaaccgctg gccttgcctg
    13441 tttgcctttt tgcactcgca acgccggctc gcttgccaca ttatgatggg tgaaagaatg
    13501 cgattcgctc ggttttcgcg cgtctcgcct gatcttgggg caaaatgatg cggaagccga
    13561 tctggatcga tacggatgtc gggtttgatg acatggccgc tctggccatg gttcattccg
    13621 atccgaactg ggcggttctc ggcctcgggc tgagttatgg caacgcccgg ctggatcagg
    13681 cggtcgctca ggcgcagcgc atgcggggct ttttcggctg gcgcacgccg ctgcatgccg
    13741 gctctgccaa gccgatcctg ggccctgcga tgacagggga ggcgctgttc ggccctaccg
    13801 gactgcgcac gatcggtcgc accctgcccg attgcgaaag cgagatcgcc cgcgaggatg
    13861 cagtgaccgg catgacccgt gcccttgccg gtgccgatgc gccggtcacc gtgctggcgc
    13921 tcgggccact gaccaacatc gccattgcgc tgctggcccg cccggagctg gcccggctga
    13981 ttgaacggat cgtctggatg ggcggcgccg cgatcggcgg caaccatacc gccacgaccg
    14041 aattcaacgc cgcctgtgat cccgaagcac tggccattgt tctggaaagc ggcgttgcgt
    14101 tccgcatgat cgggcttgaa tgctgccggc aggtcaccat caccggcacc gatgccgcca
    14161 agctacgcgc cctgcagacg gagcgggcgc ggattctggc cgatctgatg gatggatatg
    14221 tgcggctggc cagcgtcgat ggcagccgtc cgatgccggt atacgatgcg gtggctgccg
    14281 ccgccgttct cgatgaccgg gcggtggcct tcattgcggc caatatttcg gtcgagcggg
    14341 ccggcgcgct gacgcggggc acgaccatct gcgaattccg cccgcagaag gctgcgccga
    14401 atgccgagat cgcccgaacc gcgctggcca cccgcgtttt cgaccgcctg ctggccgatt
    14461 tcaccgccgc tgccgtcgcc gatccggcct ccgatacgat ttcgcgcgcg tcctgagctc
    14521 gctggtcacc cccgacagac cgggccggcg cttacagcgg cagttcctgc tcgaccaacc
    14581 gcgcaaagta gctggccccg atcggggtaa ggctgtcgtt gaaatcgaag ctctggttgt
    14641 gcagggcgat ggcactgcgc gcatccgccg cctgtcccag tttcacatag cagcccggcc
    14701 gttgcagcag catgtaggag aagtcctcac cgcccatgct ggggctatgg taggtcagca
    14761 ccttgtcggc gccgaccacg ctggctgcgg cccgtgccgc acaatcggcc tcatgcaccg
    14821 aattgaccac cggcggatac ccgcgatgat agtcgacatc cgccgccgcg ccatgcgctt
    14881 cggccgtcgt catcaccata tgggtcagca gccgctggat ttcatgctgc gtttcagccc
    14941 gcagggtgcg caccgtgccg gtcagcagcg ccttttcagg aatgacattc atcgccgagc
    15001 cggcattgaa ggtcgacaca ccgacgaccg ctgaatcgaa ggagttggtg cgccgcgaca
    15061 cgatgctttg cagagcgacg acgatatggc tgccaaccag aaccggatcg atggcgtgat
    15121 gcggccgggc cgcatgcccg cccttgccat ggatgcggat gtcgaaatga tcggctgagg
    15181 ccatgaccgg accggcaact gcggtgatga cccccagcgg caatagcgga tcggtatggg
    15241 cggcatagac cgaatcgcag gggaagcggt cgaacagccc ctcttccagc atcacccggg
    15301 cgccgccgcc gccctcttcc gccggctgaa agatgaaatg caccgtgccg gcgaagttgc
    15361 gcgtctcggc gagatagcgg gcagcggcca gcagcatcgt cgtatgcccg tcatggccgc
    15421 agccatgcat ctttccgggc accgtgctgc ggtaggccag cgtatcggcc tcctgcatcg
    15481 ccagagcgtc catatcggcg cgcaggccga tggcccggtt ggaggcgcca cgcccgcgca
    15541 ggacgccgac gacgccggtc ctggcgatag cccgatgcac ctcgatgccc cagctttcga
    15601 gtttctcggc gacgatcgcg ctggttcgat gttcctcgaa gccgagctcc gggtgggcat
    15661 gcagatcctg cctgatggca gcgaatgcgc cggcccattc ggccaggcgt ggaatgacac
    15721 tcatgtcgga ccctggtttg ccgcggcagc acagccgcag aagcgattga ggcggccttt
    15781 tagagcactt tccgaccaga ttgaacccat tgcgtttcgc gggccgcgtg gcgatccggg
    15841 cggaggctgg cgaagagcgt ttccgtcgat ccggtcgagc cagcgtccaa accgggcgcc
    15901 cgcgccccgg aaacccggag ggatgaccat tttcgggcca aatccagcga aaaatgctcc
    15961 aaccggatcg aaggatacgc ttgtcgcatt ttccttgtcc ttgacccgaa actggtcatc
    16021 gcactgatac aatctggtcg gaaagtgctc taccctgtga ttgcccgcaa caaaagctct
    16081 ctcaccggcc cttgctccag cgccaccgga ttggtcggcg cggtgggatc gttgagcgct
    16141 gcggcaacga tcgtatcagc cttgccatcg tcgatgccga tggcaccaag cgtatcggga
    16201 atgtcgagcg ccgtcctgag atccagcacc cagtcgatga cgccatccat atcctgccgg
    16261 tccaacccga gataggcagc gagccgctgc atcttgtccc ccagcaccgc gcgattgaag
    16321 tcgagcgcat agggcatcag gatcgcgttg agcagaccgt ggtgggaatc gtagatcgcc
    16381 cccagcggat gggcgagcga atggatcacg ccgagcccct tctggaacgc tgccgccccc
    16441 atggtcgccg ccaccatcat ttgcgcccgc gcctcgatgg agcggccatc ccgggttgcc
    16501 accggcagcc agtccttgat cagccgcatg ccttcgagcg cgatgccatc ggccatcgga
    16561 tgaaagccag gggcgcaata ggcttcgagg caatgggcga gcgcatccat tccggtcgcc
    16621 gccgtgatcg ccggcggaac gccgatggtc agcgcaggat cggccaccac cgtcaccggc
    16681 atgatcttcg gatggaagat cagccgtttg gtatgcgtct cctcatcggt gatgaccgaa
    16741 acgcgcccga cttccgagcc ggtgcccgag gtcgtcggca cggcgatgga cggcatgatc
    16801 ttggccacat cggcgcgcag gaaattgtca ccgacatcct cgaaatccca cagcgggcgc
    16861 gactggtgcg cgtagaaggc gatcgccttg ccggcatcga gcgccgagcc cccgccaaag
    16921 gcgatcaccc cgtcatgccc gccctcgcgg aactgccgcg cgccggcctc gacattggcg
    16981 cgcaccgggt tgggcttcac atcggcgaac accgctgtcg gcaccccggc tgcggcattc
    17041 gccgccaccg cctcggccac catcggcagt tttgccaggc cggcatcggt gaccagcagc
    17101 ggccggcgga tgttgttggc aaggcaatgc tgcgccagct ccaggatgcg gccggggcca
    17161 aacttgatcg gtgtcgggta gttccagttc gatgtgaagg acatgggtat ttccagacag
    17221 tcagagcgtg gttctgaggt gaaacgattt cggccgggtc agatgctcgt atccgacctt
    17281 tgacagggtg gcgccgcgtc ccgaatgctt gacgccggtc caggcgagtt ccggatcgag
    17341 ataatcgcag cggttcatga acaccgtacc ggtttgcaga cgatcgccca gcgcgatcgc
    17401 cgcttccgca tcgcttgtcc agatggctgc ggtcaggccg aaggcgctgt cgttcatcag
    17461 cttcagcgcc tcctcgtcgc cggcaacctt catgatgccg accaccggcc cgaagctctc
    17521 ctcgcgcatc accttcatgg tgtgatcgac ctcgacgagg atctgcggcg ccagatacgg
    17581 cgtgccggcc tgcgagcgcg ggaagctctt ttcgctgagc agagcgatcg cgccatgcgc
    17641 caccgcctcg tcgatatgcc cgcgcaccag atcggcggca gcggtgcgca ccatcggccc
    17701 cagcgtcgtc tccggccggg tcggatcgtc gagcagatag ctcctggtca gcatcaccgc
    17761 ccgctcgaca aactcgtcat agagcttttc gtggacataa atgcgctcga tgccgcagca
    17821 cgactgaccg gaattgaaga acgccccgtc gacaaggttc tcgaccgcat gggcgatgtc
    17881 ggcatcggcc cggacatagc cgggatcctt gccgccgagt tcgaggccga cgccaatgaa
    17941 ccgcccggcg gcggcccgct ccatcgcctc cccgcccggc accgaaccgg tgaagcagac
    18001 atgcgcgatc tccggcatgc cgatcagcgc ggcgaccgcc tcatggctca tgtgcagcgc
    18061 ctggagcagg cccttcggca gaccggcctc cctcgccgcc tcggcaaaac gttcggcaca
    18121 cagcggggtc tgcgccgcat gcttgagcag cacgcaattg ccggccatca gcgccggcac
    18181 caccgcattg accgccgtca gatagggata gttccacggc gccaccacca gcacgacgcc
    18241 aagcggtgcg cggcggatga agcggcggaa ccccgccttc tcgccgacgg cgatatcggc
    18301 cagcgcttca ggcgccaggg cgatcatggt gcgcgcccgc tcctcgaagc cgcgcacctc
    18361 gcccggagtg tagcggatcg gccgccccat ctgccaggtc agttcctcgg caatcgcatc
    18421 gcgtttggca acgaaggcat cgacaaaacg cgacagcgcg gcggcccgct gatcgagtgg
    18481 cacgcccgcc cattccgctt gcgccttgac ggcggcgttc agcgcccagg cgatctcggc
    18541 cgaacttgcc agcggccgct cgacatagat gcgcccatcg accggagaga tggttttctg
    18601 gatctgcatg gtctggctca gatgatctcg aaatagcggg cgagttccca gtcggtgata
    18661 tgcttgcgga attcgcgctc ctcccattcg cgcgttgcgg cgaaatggtc aacgaacgca
    18721 tcgccgaaca gcgcgcgggc gggccgggag gctttcagcc gctgcgcggc ttcccacagg
    18781 gtcgccggaa tggtcaggtt cttgggatgc ttcttgtcat aggcattgcc gatgacgatg
    18841 ggcgtcggct cgatgcgatg ctcgataccc catagccctg caccgagcgc agcggcgagc
    18901 gccagatacg gattggcatc ggccgccgcg atccggcatt cgattcgctg cgactttggc
    18961 gaaccgggca cgacgcgcag cgccgtggtg cggttctcga caccccaggt ggcgctggtc
    19021 ggcgcccagt agccggggat cagccgcgaa taggaattga cggttgagga gatcatgccg
    19081 aggatttccg gcagcagctt ctgctgcccg ccgaggaacc agcgcatggt atcgctcatg
    19141 ctgtagggct tgccggcttc atggaacacc ggcttgccgc tctttttatc cttgagcgac
    19201 agatggatat gcccgctctg gcccggccag tcgcgcgacc atttggccat gaacgtcgcc
    19261 atcatgccgc gccgctgcgc gaacaccttg gcgaaggtct tgaacagcac cgccttgtcg
    19321 gccgagcgca gcgcatcatc gaccgcgatg gccgcttcca gaacgccagg gccggtttcg
    19381 gtatgcagcc cctcgatctc gcaatccatg gcgcggaacg tgtcgagcat ctcgcggtag
    19441 agttcgctga gcacgctgga gcgcagcacc gaatagccga agaaccccgg cgtaatcggc
    19501 ttgagatcgc ggtagccctt ggcccgcacg ctctccggcg tttcgttgaa caggaagaac
    19561 tcgtattcga gcgcggcgga agcggcaaag cccatgtcgg cggcgcgcgc cagcacgcgg
    19621 cgcagggtgc cgcgcgggca gatcggctc
//
LOCUS       DAOMXG010000027        19516 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_1642_length_19516_cov_10.790144, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000027 DAOMXG010000000
VERSION     DAOMXG010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 19516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19516
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1642_length_19516_cov_10.790144"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="PLQ11_03290"
     CDS             complement(<1..619)
                     /locus_tag="PLQ11_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 associated
                     domain-containing protein"
                     /protein_id="HRK23958.1"
                     /translation="MSDSNKTGDKTIGVPATKTLSLKRPVEQGTTVRQSFSHGRTKQV
                     VVEVKHSRFPAQPAAPARPQAPAAQPPGPAKPAASSRPPVAPPAKTGVVLRTLTDEER
                     DARTQALMESRRREDEDRRRMEAEERVRREREERDRKEREAAEARKREEDDRRRAELD
                     VRRRSEEEAKRRLGDEPGAPAGAAPRGPRVEIGTPPRPAMNREAGF"
     gene            complement(612..1298)
                     /locus_tag="PLQ11_03295"
     CDS             complement(612..1298)
                     /locus_tag="PLQ11_03295"
                     /inference="COORDINATES: protein motif:HMM:NF006622.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRK23959.1"
                     /translation="MRAPEATPSTRTCIVTRQALPVDGLIRFVAGPDGQVVPDLRRRL
                     PGRGVWVTGAAEAVREAVRRNAFSRSLKAPVSASEDLVDQVGALLRRAALQALSLANK
                     AGVVITGSAKIEAAAPRGFAALLHASEAAPGGIEKLERAVRRQRRDGLAIPAIRLFTT
                     EELSLSLGREHVIHAALVTGAPSGMFLKSARAADRYWRSCSATAHASGSPAGGSVDTR
                     ISTEDSEPNE"
     gene            complement(1310..2905)
                     /gene="nusA"
                     /locus_tag="PLQ11_03300"
     CDS             complement(1310..2905)
                     /gene="nusA"
                     /locus_tag="PLQ11_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HRK23960.1"
                     /translation="MAVSANRLELLQIADAVAREKGIEREVVLQSMEDAYAKVAKQRY
                     GSETEVKAEINPRTGEIKLARLMLVVDQVENDANQIALVDARRRNPAAEVGDYLSEPL
                     PPVEFGRISAQSAKQVIVQKVREAERDRQYDEFKDRIGEIVNGVVKRVEYGNVIVDLG
                     RGEAIVRRDELIPREMFRPGDRIRSFLYDVRREARGPQIFLSRTHPQFMAKLFRQEVP
                     EIYDGIIEVKAVARDPGSRAKIAVISRDSSIDPVGACVGMRGSRVQAVVQELQGERVD
                     IIPWSQNAATFIVNALQPAEVSKVVLDEDTGRIEVVVPDDQLSLAIGRRGQNVRLASQ
                     LTGWDIDILTEAEESERRQKEFVERTSTFVAALNVDEVVGQLLASEGFRSVEEVAYVD
                     VNDLASIEGFDLETAEEIQNRARDHLARIEAENQEKRKALGVADELGEVAGVSSAMLV
                     AFGENDVKTVEDLAGCATDDLVGWSEKKGGETVHIAGILNGLPVSRDEAEAIIVAARV
                     KAGWIDEPVADEPEGVDETAEEA"
     gene            complement(2908..3564)
                     /gene="rimP"
                     /locus_tag="PLQ11_03305"
     CDS             complement(2908..3564)
                     /gene="rimP"
                     /locus_tag="PLQ11_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HRK23961.1"
                     /translation="MTGADARLIRETGLAARVAAIVEPMLSGLGFDLVRLRISGQNGC
                     TVQIMAERPDGSIGVEDCETISRAISPLLDVEDPIDREYHLEISSPGIDRPLVRLRDF
                     SRWVGHEIKVEMENLVAGRKRFRGWIEAVDGQSVVLRRLDAAAEDDPLVMLPMHDMGE
                     ARLVLTDALIREALSRDKTARKARGEPEEPETDQTGVDDETAQPPRPRRGPNRFKRRM
                     "
     gene            3718..4398
                     /locus_tag="PLQ11_03310"
     CDS             3718..4398
                     /locus_tag="PLQ11_03310"
                     /inference="COORDINATES: protein motif:HMM:NF016207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HRK23962.1"
                     /translation="MTCPATAAILLVMLYNPPHFRQTETGALLDQLATVRFATLVSNA
                     PQGPVVTHMPLIFDRQAGPMGTLIGHLARANPHWTVADLDTPSIVLVHGPDAYVSPSW
                     YPSKRETGKVVPTWNYSVLHVTGRLSIHTDPEWLLAAVSRLTDHHERDFAEPWAVMDA
                     PPAFLAAQLKGIVGISLTITGIEGKSKLSQNRPEADQAGVVAGLAEEADPGSAAVLAL
                     MRGRTVTP"
     gene            complement(4388..5092)
                     /locus_tag="PLQ11_03315"
     CDS             complement(4388..5092)
                     /locus_tag="PLQ11_03315"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine(46)-N(7))-methyltransferase TrmB"
                     /protein_id="HRK23963.1"
                     /translation="MIDSTATPPDDKAAGFFGRRKGKRLRGEQADRIDHVLPTFRLDL
                     SGPVLVPQQLFARPVEDLWLEIGFGGGEHLADNAARHPSIGFIGCEPFVNGVAKLVAE
                     IQHRALPNIRIHDGNASDVLAKLPAASIGRIDLLYPDPWPKRRQRKRRFVSDKSLKAL
                     ARVLRPGGEFRFASDIDDYIGWVLQRIARSPDFLWTAEKAVDWTTPFPDWPGTRYEAK
                     ALREGRKPSYLRLIRV"
     gene            complement(5255..6460)
                     /gene="metK"
                     /locus_tag="PLQ11_03320"
     CDS             complement(5255..6460)
                     /gene="metK"
                     /locus_tag="PLQ11_03320"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006641403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HRK23964.1"
                     /translation="MARSSYLFTSESVSEGHPDKVCDRISDEVVDLFFREAAKAGMDP
                     AHVRVACETLATTNRVVIAGEVRTSLDEKAMKSKVKSAARKAIRAIGYEQDGFHWKTA
                     KIDVLLHPQSAHIAQGVDAAGNKDEGAGDQGIMFGYACRETRDLMPAPIYYAHKILEN
                     LTAYRKLGEKGCQVLGPDAKSQVTVRYENGKPVAVTQIVLSTQHLDARLTSDDVRKIV
                     EPVIRETLSDIEIAKDCKWYVNPTGAFVIGGPDGDAGLTGRKIIVDTYGGAAPHGGGA
                     FSGKDPTKVDRSAAYAARYLAKNVVAAKLADRCTIQLSYAIGVAKPLSIYVDLHGTGK
                     VDEARLEKVLGEVMDLSPRGIRTHLGLNKPIFSKTAAYGHFGRKPGRDGSFSWERTDL
                     VDAIRKAVA"
     gene            complement(6617..7030)
                     /locus_tag="PLQ11_03325"
     CDS             complement(6617..7030)
                     /locus_tag="PLQ11_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRK23965.1"
                     /translation="MKKSPGPVDKHVGSRVRMRRILLGMSQERLGEALGLTFQQVQKY
                     EKGTNRIGASRLQQISQILGVPVTFLFEGAPLERALPEGGFAEPLQAEYSLDLLSTPE
                     GVQLAKAFMSIEDPKVRRRVVDLVATLADGQKASR"
     gene            complement(7204..8877)
                     /gene="lnt"
                     /locus_tag="PLQ11_03330"
     CDS             complement(7204..8877)
                     /gene="lnt"
                     /locus_tag="PLQ11_03330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HRK23966.1"
                     /translation="MSARLSAWSQVILLSEGASRWLIAFLAGAVGALAMAPVGLVPAF
                     AISFPVAVWLLDGATGGPSRFSPRALLAAARDGWWFGFGYFLAGLWWIGAAFLVETDE
                     FAWALPLGVVGLPAGLALFMAAGFALARLVWLPGAARILSLTAGLGLSEAARHMVLTG
                     FPWNGFGQGLGFWLVPAQIVSVIGTDGLGLLTIAIFAAPATLVDETPDGRANIRPLVL
                     AALGLVVIFGFGAARLMLSGGVLAQPQRIETVPNVRLRLVQPNMSQREKIAQQDGLAV
                     LRRLIAQSDRATGPKSTGMVDATHVIWPESPFPFLLTNEPSAMAELARVQPPSTTVLT
                     GAVRAEPGPDRSRIYYNSLVALGAKATILATYDKVHLVPFGEYLPLESVLSALGLRRF
                     VHAPGSFSLGGQRQPMELPGLPPLLPLICYEAIFPYEIDAATKRPEWMLNITNDAWFG
                     ETFGPHQHFAQARLRAIELGLPLVRVANTGISGLVDGHGRTLAASPVGAESIIDVLLP
                     KALPTTVYATVGNNIGLFGPILSLVLGGFVATLDRRRGRNRTTWTTRQR"
     gene            complement(8874..9806)
                     /locus_tag="PLQ11_03335"
     CDS             complement(8874..9806)
                     /locus_tag="PLQ11_03335"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRK23967.1"
                     /translation="MTTPDLPTESWTDRIKAALGLSERASLREGLEAALGEAAKSGDV
                     SAKEWAIVSNVLKVRNRRISEVMTPRARIVALAEAETLEEVLAAFLTSEHSRIPVYGE
                     SLDDPRGMIHLRDFLPLVTSTTQQSSQDNRRTQTLRDTALIRPLIFVPPSASPLDLLL
                     RMQTRRVHLALVINEYGETDGLVTIEDLIEAIVGDINDEHDLPTAGLTRLDEQTWSMA
                     AETPLAEAAAAIGHALPVDRNAIGVDSIGGYVTALAGRLPQPGEVIEGPGGLLFEVIE
                     ATPRQLLTLVLRLSANGETGAEEDRSGLSRGKAP"
     gene            complement(9803..10300)
                     /gene="ybeY"
                     /locus_tag="PLQ11_03340"
     CDS             complement(9803..10300)
                     /gene="ybeY"
                     /locus_tag="PLQ11_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HRK23968.1"
                     /translation="MSLEVEIVLENPDWSAIADLDGRIVHAARTALEQAAVTVQPGCE
                     LAVLLTDDAAIGALNARWRNKDMPTNVLSFPAVALDRIATSPVLGDIALAYETCAREA
                     QVEARPLVDHILHLVVHGVLHLVGYDHQTEAEATTMEDLERTILARLGVPDPYSAADS
                     SGASL"
     gene            complement(10305..11312)
                     /locus_tag="PLQ11_03345"
     CDS             complement(10305..11312)
                     /locus_tag="PLQ11_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRK23969.1"
                     /translation="MSFEVSDGRHGGEWVTSLTLDDNRLASLVFGQYDQNIAHLERRL
                     SIVATVNGNHIVLKGAKEAAEQAKRVLKALYAQVKSGRPVSQGDLDGAIAESVLQGSL
                     FPAADTGKTGFNQIITRKRGAVKARNGAQDAYLRALQQHELVFADGPAGTGKTWLAVG
                     HAVQLLEQGKVERLILSRPAVEAGERLGFLPGDMKDKVDPYLRPIYDALHDFMEGRLV
                     ERGLQTGMIEVAPLAFMRGRTLTNAAILLDEAQNTSSMQMKMVLTRLGENSRMIVTGD
                     PTQIDLPPGQKSGLIEAMAILRGVEGIGHVSFGHGDVVRHDLVRRIVRAYEDHGGGAK
                     V"
     gene            complement(11309..12697)
                     /gene="miaB"
                     /locus_tag="PLQ11_03350"
     CDS             complement(11309..12697)
                     /gene="miaB"
                     /locus_tag="PLQ11_03350"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017486446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HRK23970.1"
                     /translation="MPAAAAPSPRRVHIKSYGCQMNVYDATRMADALAGEGYAEVAAA
                     EDADLVILNTCHIREKAAEKVYSELGRLRRLKQERASAGEDTRIVVAGCVAQAEGAEI
                     MKRAPVVDVVVGPQSYHRLPELVAEARQNRRALDTDFPAEDKFDHLPAPTRAQIAARG
                     VSAFLTVQEGCDKFCSFCVVPYTRGAEISRSVDRIVSEAERLASAGVRELTLIGQNVN
                     AYHGEGPDGQTWSLAALARRLAVIPGLDRLRYSTSHPADMGEDLIALHRDLPAFMPYL
                     HLPVQSGSDRILAAMNRRHTAADYCRLIERIRVARPDIALSSDFIVGFPGETEADFAQ
                     TMRLVETIGFASAFSFKFSPRPGTPAAEQADQLPEAVKSERLAVLQDLLTRQQKAFNA
                     ATIGRTVDVLFEKPGRHPGQIAGKSPYLQAVQVDAPVDWIGRIVPVKITGQATFSLFG
                     RLMPEASGEDIT"
     gene            complement(12758..13528)
                     /locus_tag="PLQ11_03355"
     CDS             complement(12758..13528)
                     /locus_tag="PLQ11_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRK23971.1"
                     /translation="MSRAGSLRRIARLMALAPAFLIGLPLQALAVRLNSRAARILPLW
                     FHRYACRVLGIRRQVSGPLPAGGSVLLVANHVSWLDIIVIGATLPISFVARADLAGWP
                     GAGLLARLQRSVFVDRTRRKATRPATGEIAQRLAGGEAIVLFAEGTTSDGNRVLPFRP
                     ALLAAADGSAPVQPLVLHYRSLDGLPLDRRTRPLVAWYGDMDLVPHLGELFRRGPVDV
                     ALHFGSPVHQGGDRKMLARRLEAEIRATAARLRLGRDV"
     gene            complement(13525..13899)
                     /locus_tag="PLQ11_03360"
     CDS             complement(13525..13899)
                     /locus_tag="PLQ11_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HRK23972.1"
                     /translation="MTEQRRVIARVIEAAEDHPDVEELHRRAAMVDSGISLSTVYRTV
                     RLFEEAGIIARHDFREGRARYEQTPDEHHDHLINVRTGAVIEFRSAEIEALQAEVARR
                     LGFRLVDHRLELYAVPLDENQT"
     gene            complement(14005..14514)
                     /gene="rimI"
                     /locus_tag="PLQ11_03365"
     CDS             complement(14005..14514)
                     /gene="rimI"
                     /locus_tag="PLQ11_03365"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HRK23973.1"
                     /translation="MRWFGLSRRRQAALRPARMDDARAIAELHSLSFSHPWPTLTVES
                     LLADRTVRAHVADNGRIVGFLLLRVAADEAEVLSIGVAPSARARGLGRRLMEAGLDGL
                     VFDRIRLVFLEVETENLGALKLYQRLGFELIGRRKGYYRGSDGISRDALTMRLDISSR
                     SPATPLLDG"
     gene            complement(14514..15197)
                     /gene="tsaB"
                     /locus_tag="PLQ11_03370"
     CDS             complement(14514..15197)
                     /gene="tsaB"
                     /locus_tag="PLQ11_03370"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017486442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HRK23974.1"
                     /translation="MRVLALDTALGATSVAVVDYPSGTVLARASLPMQRGHAEALMPM
                     IEQAMAGVEGGFGSLSRIGTTVGPGSFTGLRVAVSAARSLGLALAIPVVGVSTLAAYA
                     APVIASGARSMTAVAIDARHGAVFFESFWLDGKKALGPLVLGLEQAAETLRSGRCRLV
                     GTGAPALHDHARKLGLEADLIETGPAPDILWVARLAAAADPACAPARPLYLKAASAVP
                     QGHGQIARA"
     gene            complement(15213..15770)
                     /locus_tag="PLQ11_03375"
     CDS             complement(15213..15770)
                     /locus_tag="PLQ11_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HRK23975.1"
                     /translation="MFIQTEVTPNPATLKFLPGKAVMHQGTLDIRSAADAAASPLAGR
                     LFSIPGVTGVFFGSDFVTVTKAAGEWQHLKPAILGAIMEHYLSGAPLIDGVAPPQHEG
                     DEFYDPKDADTVATIKELLDTRIRPAVAGDGGDITFRGFEKGVVYLAMKGACSGCPSS
                     TATLKHGIQNLLKHFLPDVQSVEAI"
     gene            complement(15861..16898)
                     /gene="trpS"
                     /locus_tag="PLQ11_03380"
     CDS             complement(15861..16898)
                     /gene="trpS"
                     /locus_tag="PLQ11_03380"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRK23976.1"
                     /translation="MALVPPRVFSGVQPTGNLHLGNYLGAIRRFVTLQDTHDCIYCVV
                     DLHAITVWQDPEDLIRATREVTAAFLAAGIDPGRHIVFNQSRVAEHAELAWVFNCVSR
                     LGWLNRMTQFKEKAGKDRENASVGLYAYPNLMAADILVYRATHVPVGEDQKQHLELTR
                     DIAQKFNNDFAARIAALGHGEGYFPLPEPLIAGPAPRVMSLRDGTKKMSKSDPSEYSR
                     INLNDDADAIAQKIRKAKTDPEPLPGEIEGLAGRPEADNLVTIFAALSGRERAAVLSE
                     YGGGQFSTFKAALADLAVSSLAPIAGEMRRWLADPASIDAVLRNGGDRARAIASSVMD
                     DVKDIVGLVSR"
     gene            complement(16998..18560)
                     /gene="murJ"
                     /locus_tag="PLQ11_03385"
     CDS             complement(16998..18560)
                     /gene="murJ"
                     /locus_tag="PLQ11_03385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HRK23977.1"
                     /translation="MSLVRSYTLVGSATLASRLTGFVRDILIAAVLGSGRVADAYLAA
                     FLLPNLFRRVLSEGAFNAAFVPILSRRRTEGDASARDFVRAAFAAILALAFGLVLVAE
                     LSMPGIIKVIAPGFVADPEKFAQAVLFGRIAFGFVGAVIVSAFLASLLNAVGRYALVA
                     MAPLILNGLLIPVLLALFVLGWQGDVRVGWVLVFSVLAAGFLQLAYVAYGVRRAGIVI
                     WPLRWEADRDVRLMIAMVLPAVVLAGAGHFNMVVAAQLSSALPSAVSWIYYADRIFQL
                     PLGFVAAAIGVVLLPEVSRAIRDGDHPRADDASNRALEFGMLLVLPAMLALVILARPI
                     IDIIYRHGAFTASDSAAVADMLSALALGLPGFVLVKVFLPAFLARESLRLPLLAALAG
                     VVANIAFTLALVGRLGPVAAPLGVATSAWVNALLLGIGLKMRGVFRLDGRATRSLPLI
                     VVAAALTGAVTYANLVLFEPWLAPDRALALRTGALALVCLSGVGVHAVASHLFGIADL
                     GGLRRAFGRTGA"
     gene            complement(18576..>19516)
                     /gene="hslU"
                     /locus_tag="PLQ11_03390"
     CDS             complement(18576..>19516)
                     /gene="hslU"
                     /locus_tag="PLQ11_03390"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HRK23978.1"
                     /translation="AKAELAAEERVIDALVGATASPGTRDSFRRRLRAGELNDKDIDI
                     EVSSPAPAIPMLEMPGAQPGQAALGELLGRAFGKPMRKRRTTVAEAHSPLVQEESDKL
                     IDHDQIVQDALREVENNGIVFLDEIDKICARENRGGADVSREGVQRDLLPLIEGTTVS
                     TKHGAVRTDHILFIASGAFHVSKPSDLLPELQGRLPIRVELTPLARSDFRRILTETEA
                     SLVKQYVALLGTEQVELSFTDDALDAIADAAFEVNATIENIGARRLQTVMERILDEIS
                     FSAPDKSGQKVVIDGAQVQASLGDLARNADLSRFIL"
BASE COUNT         3832 a         6316 c         6103 g         3265 t
ORIGIN      
        1 ggaatccggc ttcccggttc atcgcagggc gaggcggggt gccgatctcg acccgcggac
       61 cgcgtggcgc agcgccagcc ggagcgcccg gttcgtcacc aaggcggcgc ttggcctcct
      121 cttccgagcg acgacggaca tcaagctcgg cacgacggcg atcgtcttcc tcacgcttgc
      181 gggcctcggc agcctcacgc tccttgcggt cgcgctcttc gcgctcgcgc cggacccgtt
      241 cctcggcttc catacggcga cggtcctcat cctcacgccg gcgcgattcc atcagcgcct
      301 gggtgcgggc atcacgctcc tcgtcggtca gcgtgcgcag aaccaccccc gtcttggccg
      361 ggggtgccac cggtggacgc gaggatgccg ccggcttggc gggccctggc ggttgagcag
      421 ccggggcctg tgggcgagcg ggcgcagcag gctgagccgg aaagcgactg tgcttgacct
      481 cgaccacgac ctgcttggtg cggccatggg agaagctctg gcgaacagtg gtgccctgct
      541 cgaccgggcg cttaagcgaa agcgtcttgg tcgcgggcac tccgatggtc ttgtcgccgg
      601 tcttgttgct gtcactcatt aggctcagag tcctcggtcg aaatacgtgt atcgacgctc
      661 cctccggccg gggagccgga agcgtgcgcc gttgcggagc aactccgcca atatcggtcc
      721 gccgcgcgtg ccgatttcag gaacattccc gaaggggcac ccgtaacgag cgctgcatgt
      781 atcacatgtt cgcgtccaag cgacagactt aattcttcgg ttgtaaagag tcgaatcgcc
      841 ggtattgcaa gcccgtcacg gcgctgccgc cgcaccgccc gctccagctt ttcgatcccg
      901 cccggggctg cctcgctggc gtgcagcagc gcggcaaaac cgcgcggagc cgccgcctcg
      961 atcttggcag agccagtgat cactactccc gccttgttgg ccagcgaaag cgcctgcagc
     1021 gccgcgcggc gcagcagggc gccaacctga tcgacaagat cctccgatgc tgacaccggt
     1081 gccttgagcg agcgggaaaa ggcgttgcgt cgcaccgctt cgcgcaccgc ctcggccgca
     1141 ccggtcaccc agacaccacg gccaggcaac cggcgccgga gatcgggaac cacctggcca
     1201 tccggccctg caacgaaccg gatcaatccg tcgaccggca aggcctgccg ggtcacgatg
     1261 caggtgcggg tcgagggtgt tgcttccggt gcgcgcacga tgtctccgat caggcctctt
     1321 cggcggtctc gtccacgcct tcgggctcat cggcaaccgg ctcatcgatc cagcctgcct
     1381 tgacgcgggc agcgacgatg atcgcctcgg cttcgtcgcg ggagaccggg aggccattga
     1441 gaatgccggc aatgtgaacc gtctcgccac ccttcttctc gctccagccg accagatcgt
     1501 ccgtggcgca cccggcaaga tcctcgaccg tcttgacgtc attctcacca aacgcaacca
     1561 gcatggccga ggaaacgccg gcaacctcgc ccagttcgtc agcaacgcca agcgccttgc
     1621 gcttttcctg gttctccgcc tcgatacggg caaggtgatc gcgggcacgg ttctggattt
     1681 cctcggcggt ttcgagatcg aaaccttcga tggacgccag atcattgaca tcaacgtagg
     1741 ccacctcctc gaccgagcgg aagccttcgg aggccagcaa ctgaccgacg acctcatcga
     1801 cattgagggc cgcgacgaag gtggaggtgc gttcgacaaa ttccttctgc cggcgctcgg
     1861 attcctcggc ctcggtcagg atgtcgatgt cccagccggt gagctgggag gccaggcgca
     1921 cgttctggcc acgccggccg atcgccagcg agagctgatc atcggggacc accacctcga
     1981 tacgtccggt atcctcatcg agcaccacct tcgacacctc ggccggctgc agcgcattga
     2041 cgatgaaggt ggcggcattc tgcgaccacg ggatgatgtc gacgcgctcg ccctgcagct
     2101 cctgcaccac cgcctgcacg cggctgccgc gcatgccgac gcaggcgccg accggatcaa
     2161 tcgacgaatc acgggaaatc accgcgatct tggcgcgcga tccgggatcg cgggccaccg
     2221 ccttgacctc gatgataccg tcatagatct ccggcacttc ctgacggaac agcttggcca
     2281 tgaactgcgg atgggtgcgc gacaggaaga tctgcggccc gcgcgcctcg cggcgcacat
     2341 cgtaaaggaa ggagcggatg cggtcgccgg ggcggaacat ctcgcgcggg atcagctcgt
     2401 cgcggcgcac gatcgcctcg ccacgcccca gatcgacgat cacattgccg tattcaaccc
     2461 gcttgacgac gccgttgacg atttcgccga tgcggtcctt gaactcgtca tactggcgat
     2521 cgcgctcggc ctcgcgcacc ttctgaacga tcacctgctt ggccgactgg gcggaaatgc
     2581 ggccgaattc gaccggcggc aatggctctg acagatagtc gccgacctcg gcagctggat
     2641 tgcgccgacg cgcatcgacc agcgcgatct ggttggcgtc attctcgacc tgatcgacca
     2701 ccagcatcag ccgggcgagc ttgatctcgc cggtgcgcgg gttgatctcc gccttgacct
     2761 cggtctcgga gccgtagcgc tgcttggcaa ccttggcata ggcatcctcc atcgactgca
     2821 ggaccacctc gcgctcgatg cccttctcgc gggcgacagc gtcggcaatc tgcaacagtt
     2881 ccagtcggtt ggcagaaacg gccatggtta catcctccgt ttgaatcggt tggggccgcg
     2941 gcgcggcctg ggcggttggg cggtttcgtc gtccacacca gtttgatcgg tctcaggctc
     3001 ttcaggctcg ccacgggcct tgcgcgccgt cttgtcacgg ctgagcgcct cgcggatcag
     3061 ggcatcggtc agcacaaggc gcgcttcgcc catgtcatgc atcggcaaca tcacaagcgg
     3121 atcatcctcg gcggcggcat caagacggcg gaggacgacg ctctggccat caaccgcctc
     3181 gatccagccg cggaaacgct tgcggcccgc cacgaggttt tccatttcca ccttgatttc
     3241 gtgcccgacc cagcgcgaaa aatcgcgcag gcgaaccagc gggcggtcaa tgcccggcga
     3301 ggaaatctca aggtggtatt cgcggtcgat cggatcctcg acatcgagca gcggcgaaat
     3361 ggctcgcgaa attgtctcgc aatcctccac cccgatgctg ccgtccggcc gttcggccat
     3421 gatctgtaca gtgcagccgt tctgacccga gatgcgcagg cgcacgagat cgaagccaag
     3481 cccgctcaac atcggctcga caatggcagc gacgcgcgcg gccagtcccg tttcccgtat
     3541 caaccgtgcg tcggcaccgg tcatgccttg tcctcaaaac gaaaagagcg ggtccccgga
     3601 gggcccactc tcaaaatccg atcctgatga tcagcctgag atttggtgat tgccgctcct
     3661 catagcctga tttggacgtt tgaacaagag gcgaagccgg ctgcccgggc cggcggcttg
     3721 acctgcccgg cgacggcagc cattctcctc gtcatgctct ataaccctcc gcacttccgg
     3781 cagaccgaga ccggtgctct cctcgatcag ctggccaccg tccggttcgc aactctggtg
     3841 tccaacgctc cgcaagggcc ggttgtcacg catatgccgc tgatcttcga tcgccaggcg
     3901 gggccgatgg gcacgctgat cggccatctg gcgcgcgcca atccgcactg gaccgtcgca
     3961 gatctcgata cgccatcgat cgtgctggtt cacggaccgg atgcctatgt cagcccgtcg
     4021 tggtatccga gcaagcgcga gaccggcaag gtcgtgccga cctggaatta ttccgtgctg
     4081 catgtgacgg gtcgcctgag catccatacc gatccggagt ggctgcttgc ggcggtatct
     4141 cgactgaccg atcatcatga gcgcgacttt gccgagccat gggcggtcat ggatgcgccc
     4201 cccgcgtttc tggcagccca gctcaagggc attgtcggga tttcgcttac aatcaccgga
     4261 atcgagggca agtccaagct gtcgcagaat cgtcccgaag ccgatcaggc cggtgtcgtc
     4321 gcaggacttg ccgaggaagc ggatcccggc agcgcggcgg ttcttgccct gatgaggggc
     4381 agaaccgtca caccctgatc aagcgcaggt aactcggctt gcggccttca cgcagggcct
     4441 tggcctcata gcgggtgcct ggccaatccg gaaacggcgt cgtccagtcg accgctttct
     4501 cggccgtcca gaggaaatcc gggctgcggg cgatgcgctg gagcacccag ccgatgtaat
     4561 cgtcgatgtc cgatgcaaag cgaaactcgc cgcccggccg caacacccgg gccagcgcct
     4621 tcagcgattt atccgaaacg aaacgccgct tgcgctgacg acgcttgggc cagggatcag
     4681 ggtaaagcag atcgatgcgg ccaatcgatg ccgccggcag tttcgccaat acgtcggaag
     4741 cgttgccgtc atggatgcgg atgttcggaa gggccctatg ctggatctcg gccaccagct
     4801 tggcaacgcc gttgacaaag ggctcgcaac caatgaagcc gatcgaggga tggcgggccg
     4861 cattgtctgc gaggtgctcg ccgccaccaa agccgatctc gagccacaag tcctcgaccg
     4921 ggcgggcaaa caactgttgc ggaaccagga caggccctga cagatccagc cggaaggtgg
     4981 gcaggacatg atcaatccgg tccgcctgct caccgcgcag acgcttgccc ttgcggcgac
     5041 cgaagaagcc ggcggccttg tcatcggggg gagtggcagt gctgtcgatc atgagacagg
     5101 gccatggcaa aggtctcggt gcgcgtcaat ggcgcagccg tcctgcccgg caagcggttc
     5161 gaacgcgcga ccgcctcgcc ggtatggcgg tcgatgcgca aaaaagaagc cgccctccgg
     5221 gcggcttcgg attcggtcag cttgcgccgg aggatcaggc caccgccttc ctgatagcgt
     5281 cgaccagatc ggtcctttcc caggaaaagc tgccatcgcg cccgggcttg cggccgaaat
     5341 gaccgtaggc ggcggtcttg gaaaaaatcg gcttgttgag tccaaggtgg gtgcggatgc
     5401 cgcgtggaga caggtccatc acttcgccga ggaccttttc aagccttgcc tcatccacct
     5461 tgccggttcc gtgcagatca acatagatcg acagcggctt ggcgacgccg atggcatagc
     5521 tgagctggat ggtgcaacgg tctgccagct tggccgccac tacattcttg gcaagatagc
     5581 gcgccgcata ggcggcagag cgatcaacct tggtcggatc cttgccagag aaggcaccgc
     5641 cgccatgcgg agcggcgccg ccgtaggtgt caacgatgat cttgcggccg gtgaggccgg
     5701 catcgccgtc gggaccgccg atcacgaagg cgccggtggg attgacatac catttgcaat
     5761 ccttggcgat ctcgatgtcc gacagggtct cgcggatcac cggttcgaca atcttgcgca
     5821 cgtcatcgga ggtcaaccgg gcgtcaaggt gctgagttga cagaacgatc tgggttacgg
     5881 ccaccggctt gccattttcg taacgaaccg tcacctggct cttggcatcc ggtccaagca
     5941 cctgacaccc cttctccccc agcttgcgat aggcggtcag gttttccagg atcttgtgag
     6001 cgtagtagat cggggctggc atcagatcgc gggtttcgcg gcaggcatag ccgaacatga
     6061 tgccctgatc gccggcgccc tcatccttgt tgccggccgc atcaacgcct tgggcgatat
     6121 gcgccgattg cggatgcagc agaacgtcga tcttggccgt cttccagtga aatccgtcct
     6181 gctcgtagcc aatggcgcgg atggccttgc gggccgcgga cttgaccttg gacttcatcg
     6241 ccttctcgtc cagcgaggtg cggacctcgc cggcgatgac gacacggttg gtggttgcca
     6301 gcgtctcgca tgcgacgcgc acatgggcag ggtccatgcc tgccttggcg gcctcgcgga
     6361 agaacagatc gaccacctcg tcggaaatcc ggtcacatac cttgtcggga tggccttcgg
     6421 agacggattc ggacgtgaac agatatgagg accgagccaa gtcaaagctc cctgaaaagc
     6481 tgatggggac cgcctgcaaa cccaaaaaag gcaggcgcca gaccggcggc ctctctgccg
     6541 gtctgcggcg caaaggtcaa ggcgaaaaaa agaacccgtt ctgattttcg gccgaaattt
     6601 tcgttaaacg ggacgctcag cggctggctt tctggccatc ggccagcgtt gcaaccaaat
     6661 cgacaacccg tcggcgcacc ttgggatcct caatgctcat gaaggccttg gcaagctgga
     6721 ccccttcggg tgtcgacagc aggtccagcg aatattctgc ctgcagcggt tcggcaaagc
     6781 ctccctccgg caaggcccgt tccagaggcg cgccctcgaa caggaaagtg accggcacac
     6841 caagaatctg tgaaatctgc tgcaggcggc ttgcgccgat gcggttggtt cccttctcgt
     6901 atttctggac ctgctggaag gtgagtccca atgcctcacc aaggcgctcc tgactcatcc
     6961 cgagaagaat gcgccgcatc cgcacgcgag agccaacatg cttgtccact gggccgggcg
     7021 attttttcat ttgtttccct cagctcgcca ttcctagagc actaccagcg aattccaatt
     7081 cacagaagat gctctatatt tttgtttttt cgcattttct taacgcgaac cggaatccac
     7141 ttcgcttgaa aatgctctgg tgtgcgtcag cctggcaggt tccgaactga cgcaaggtct
     7201 tccttaccgc tgtctcgtcg tccacgttgt ccggttccgt cccctgcgtc ggtcgagcgt
     7261 agcaacaaac ccgccgagca ccaacgacag tatgggtccg aacaacccga tgttattgcc
     7321 gactgtcgca tatacagtcg ttggcaaggc tttgggaagc aaaacatcaa ttatgctttc
     7381 tgcccctacg ggactggccg caagcgtgcg gccatgacca tccacaagcc cggaaatgcc
     7441 agtgttggca acgcggacca gcggcaaccc gagttcgatg gcacgcaagc gcgcctgggc
     7501 aaaatgctgg tgcgggccga aagtttcgcc aaaccacgca tcattggtga tgttcaacat
     7561 ccactccggg cgcttggtcg ctgcatcgat ctcgtagggg aagatcgcct catagcagat
     7621 cagaggcagc agtggtggca ggccgggcag ttccatcggc tggcgctggc caccgaggga
     7681 aaagctgcca ggagcgtgga caaagcgtcg caggccgagc gccgaaagca ccgattcaag
     7741 cggaaggtat tcgccgaagg gaaccagatg caccttgtca taggttgcca gaatggtcgc
     7801 cttggctccc agcgcgacca gcgaattata gtagatgcgg ctgcggtccg gccccggttc
     7861 agcgcgcacc gcaccggtca ggaccgtcgt cgacggcggc tggacccgcg ccagttcggc
     7921 catcgccgat ggttcattcg tcaacaggaa gggaaatggc gattcgggcc agatcacatg
     7981 ggttgcatcg accataccgg tcgatttcgg gccggtggca cgatccgatt gcgcgatgag
     8041 acggcgaagc accgccaggc cgtcctgctg ggcgatcttt tcgcgctgcg acatgttggg
     8101 ctgaaccagg cgaagtcgca cattcgggac cgtttcaatg cgctgcggct gtgccagcac
     8161 gccgccggac aacatcagcc gggcagcacc aaagccaaag atcacgacaa ggccaagtgc
     8221 agccagaacc agtggacgga tgttggcgcg cccgtccggg gtttcgtcaa ccagggttgc
     8281 cggagctgcg aagatggcga tcgtcagcag cccgaggccg tcggtgccga tcaccgagac
     8341 gatctgtgcc ggcaccagcc agaagccgag cccctgaccg aaaccgttcc agggaaagcc
     8401 ggtcagcacc atgtgacgtg cagcctcgct caggccaagg ccggcggtca gcgacaggat
     8461 tcgcgcggcg cccggcagcc agacgaggcg ggcaagggca aagccggccg ccatgaacag
     8521 cgcaagaccg gccggcaacc caacgacgcc caatggcagc gcccaggcaa attcgtcggt
     8581 ttccaccaga aatgccgcgc cgatccacca caggccggca aggaaatagc caaatccgaa
     8641 ccaccagccg tcacgagccg ccgccagcag ggcacgcggc gaaaagcgcg atggtccgcc
     8701 ggttgctcca tcgagcagcc acacggcgac aggaaaactg atggcaaagg ccgggaccag
     8761 tcccacagga gccattgcca aagcgccaac ggcgccggcg aggaaggcga tcagccagcg
     8821 actggcccct tccgacagca ggatcacctg gctccaggca gacaggcgcg cgctcatggt
     8881 gccttgcccc ttgatagccc gctgcggtct tcttcggcgc ccgtctcacc gtttgctgac
     8941 aggcgcagca cgagcgtcag gagctggcgc ggcgttgcct cgatcacctc gaacagaagg
     9001 ccgcccgggc cctcgatcac ctcacccggc tgcggcaggc gaccggccag cgccgtgaca
     9061 tagccgccga tactgtccac gcctatggcg ttgcgatcga cgggcagggc gtgccctatt
     9121 gcagcggccg cttcggccag cggcgtttcg gccgccatcg accaggtttg ctcgtccagc
     9181 ctggtcagac ctgcggtcgg aagatcgtgt tcatcattga tgtcgccgac gatcgcctcg
     9241 atgaggtctt cgatggttac cagaccgtcg gtctcgccgt attcgttgat gaccagggcg
     9301 agatgcacac ggcgcgtctg catgcgcagc aggagatcga gcggcgatgc cgagggtggc
     9361 acgaaaatca agggcctgat caatgccgta tcgcgcagcg tctgcgtccg gcgattgtcc
     9421 tgcgacgatt gctgcgtggt cgaggtgacc aagggcagga aatcacgcag atggatcatg
     9481 cctctgggat cgtcgagact ctcgccgtaa acaggaatgc gggaatgctc ggatgtcagg
     9541 aaggccgcca gaacctcctc aagggtctct gcctcagcca aggcgacgat gcgggcgcgc
     9601 ggggtcatga cctcggaaat gcgccggtta cggaccttga gcacgttgga aacgatggcc
     9661 cattccttgg ccgacacatc acccgatttg gccgcctcgc ccagagcagc ctcaaggccc
     9721 tcgcgcaacg aggcgcgctc tgacaggccc agggcggcct tgatccggtc tgtccagctt
     9781 tcagtcggaa gatcgggcgt cgtcacaggc tagctccaga cgaatcagcc gccgaatagg
     9841 ggtccggcac cccaagacga gcaaggattg tccgctccag gtcctccatt gtggtggcct
     9901 cggcttcggt ctggtgatcg tagccgacca gatgcagcac accatggacg accagatgca
     9961 ggatgtgatc gacgagcggg cgggcctcaa cctgcgcctc gcgagcgcag gtctcatagg
    10021 cgagtgcgat atcgccaagc accgggctgg tcgcaatccg gtccagggca acagcgggaa
    10081 aggacaggac attggttggc atgtccttgt tgcgccaacg ggcattgagg gcgccaatgg
    10141 ccgcatcgtc ggtcagcagg acggccagtt cacagccagg ctgcacggtg accgccgcct
    10201 gttccagagc cgtcctggcc gcatgaacga ttcgaccgtc gaggtcggca attgccgacc
    10261 aatcggggtt ctcaaggacg atttcaacct ccaggctcat cggctcagac cttggctccg
    10321 ccaccatgat cctcataggc ccgcacaatc cgccgcacga gatcgtggcg cacgacatcg
    10381 ccatggccga aactgacatg gccgatgccc tcgacgcccc tgaggatcgc catggcctcg
    10441 atcaggccgg atttctggcc gggcggcagg tcgatctggg tgggatcgcc agtcacgatc
    10501 atgcgcgaat tctcgccgag ccgggtcaga accatcttca tctgcatgga cgatgtgttt
    10561 tgcgcctcgt ccagcaggat tgcggcgttg gtcagggtgc gcccgcgcat gaaggcaagc
    10621 ggcgccacct caatcatccc cgtctgcaag ccgcgttcga cgagccggcc ttccatgaaa
    10681 tcatgcaggg catcgtagat cggacgcaga tagggatcga ccttgtcctt catgtcgccg
    10741 ggaaggaagc caaggcgctc tcccgcctcg accgccgggc gcgacaggat cagacgctcg
    10801 accttgccct gctcgagcaa ttgcaccgcg tggcccaccg caagccaggt cttgccggtt
    10861 ccggccggtc cgtcggcaaa caccagttcg tgctgctgga gcgccctgag ataggcgtcc
    10921 tgcgcaccat tgcgggcttt gaccgcgccg cgcttgcggg tgatgatctg gttgaagccg
    10981 gtcttgccgg tgtcggcggc agggaacagg ctgccctgca gcacgctttc ggcaatggca
    11041 ccatcaagat cgccctgact gaccggccgg cctgacttga cctgggcata aagcgccttg
    11101 agcacgcgct tggcctgttc ggccgcctcc tttgccccct tgagcacgat gtggttgcca
    11161 ttgacggtgg caacaatgct tagccggcgc tccagatggg cgatgttctg gtcatactgg
    11221 ccaaacacga gactggcgag gcgattgtca tccagcgtca gactggtcac ccattcgccg
    11281 ccgtggcgac cgtccgagac ctcgaagctc acgtgatgtc ctccccagag gcttccggca
    11341 tcagccggcc gaacaggctg aacgtcgcct gcccggtgat cttaaccggc acaatgcggc
    11401 cgatccaatc gactggtgca tccacctgca cggcctgaag atagggtgac ttgccggcga
    11461 tctggcccgg atggcggccg ggcttttcaa acaaaacatc gacggtgcgg ccgatggtgg
    11521 cggcattgaa tgccttctgc tgacgggtca gcagatcctg caggacagcg agccgctcgg
    11581 acttgaccgc ctcgggcagc tgatcggcct gctcggccgc aggggtgccg gggcgcgggc
    11641 tgaatttgaa cgaaaatgcc gaggcaaagc cgattgtctc gaccagccgc atggtctggg
    11701 cgaaatcggc ctccgtctca ccgggaaagc cgacaatgaa atccgaactg agggcaatat
    11761 cggggcgggc gacacggatc cgctcgatca gccggcaata atcggcggcg gtatggcggc
    11821 ggttcatcgc ggccagaatg cggtccgaac cggactgaac gggcagatgc agatagggca
    11881 tgaaggcagg cagatcacga tgcagggcga tcagatcctc gcccatgtcg gcaggatgcg
    11941 aggtcgaata gcgcagccgg tcaagccccg gaatgacggc aagcctccgg gccagggcgg
    12001 ccagcgacca ggtctgccca tcggggccct cgccatgata ggcattgacg ttctggccga
    12061 tcagcgtgag ttcgcgaacg ccggccgagg ccagccgctc ggcctcgctg acaatgcggt
    12121 cgacggagcg cgagatttcg gcgccgcgcg tgtagggaac cacacagaag gaacagaact
    12181 tgtcgcaccc ttcctgcaca gtcaggaagg ccgaaacccc gcgcgcagca atctgcgccc
    12241 gggtcggggc cggcaggtga tcgaacttgt cctcggcggg aaagtccgtg tcgagggcac
    12301 gccggttctg ccgggcctcg gccaccagct caggcaggcg atgatagctc tgcggaccga
    12361 cgacaacatc aactactgga gcgcgcttca tgatctcggc gccttcggcc tgggccacgc
    12421 agccggcaac gacgatgcgg gtgtcctctc cggcggaagc ccgctcctgc ttgagccgtc
    12481 gcaggcgtcc cagttcggaa tagacctttt cggcggcctt ttcgcgaatg tggcaggtat
    12541 tgaggatgac cagatcagcg tcctcggccg cggcgacttc ggcatagccc tcgccagcca
    12601 gtgcatcggc catgcgagtg gcgtcgtaga cattcatctg acagccgtag gatttgatat
    12661 ggacgcgacg cggggacggc gccgcagcgg ctggcatggc ggggggagag ggcaaatcag
    12721 gcatgttgtt cttctttacg gcgctttggc tcaagcatca aacatcccgg ccaagtcgca
    12781 ggcgggcagc ggtggcgcgg atctccgcct cgaggcggcg ggccaacatc ttgcgatcac
    12841 ccccctggtg tacgggcgat ccgaaatgca gagccacatc gacggggccg cgcctgaaca
    12901 attcgcccag atgcggaacc aggtccatgt cgccatacca ggcaaccagc ggccgggtgc
    12961 gtcggtcgag cggcagaccg tcgagcgagc gatagtgcaa caccagcggt tgaaccggcg
    13021 cggacccatc cgcagccgcc agcagcgccg gccggaaggg caatacgcgg ttgccgtccg
    13081 acgtcgtgcc ttcggcaaac agcacgatcg cctcaccccc ggcgaggcgc tgcgctatct
    13141 cacccgtggc tggccgtgtc gccttgcgac gcgtccgatc gacaaagacc gaacgctgga
    13201 gacgggcaag cagtcctgct cccggccacc ccgccagatc ggcccgcgca acaaaggata
    13261 tgggcagcgt cgcgccgatg acgatgatgt cgagccagga gacatgattg gcaacgagca
    13321 gcaccgatcc ccccgctggc agcgggccgc tgacctgccg ccggattcca agaacccggc
    13381 aggcatagcg gtgaaaccac agcggcagga tgcgggccgc ccgactgttc agtcgcaccg
    13441 caagggcctg caaggggagg ccgatcagaa aggcgggggc cagtgccatc agccgcgcga
    13501 tccggcgcag cgacccggcc cggctcatgt ctggttctcg tccagcggca ccgcgtatag
    13561 ctcaagccgg tgatcgacaa ggcgaaaacc cagccggcgc gccacctcgg cctgcagggc
    13621 ctcgatctcg gctgagcgaa attcgatgac cgcgccggtt cggacgttga tcagatggtc
    13681 gtggtgctcg tcgggtgtct gctcgtagcg ggcccgccct tcgcggaaat catggcgggc
    13741 aatgatgccg gcctcttcga acaaccgcac ggtgcgatag acggtcgaca gcgaaatgcc
    13801 cgaatcgacc atcgccgccc ggcgatgcaa ctcctcgaca tcggggtggt cttccgcagc
    13861 ctcgatgacg cgcgcgatga cgcgccgctg ctcggtcatc ttgagaccgt gggcgatgca
    13921 tcgttcttcg aggctgcggc tgtcgctggt caaggccggt ctgccaggtt cgatctatcc
    13981 gcgtggcttg taaagccaac cgctctatcc gtccagcaac ggggtggccg ggctgcgtga
    14041 ggaaatgtcg agtcgcatcg tgagggcatc ccggctgatc ccgtcgctgc cgcgataata
    14101 gcccttgcgt cgcccgatca gttcaaagcc caggcgctga taaagcttga gagcgcctag
    14161 attttctgtc tccacctcaa gaaaaaccag acggatccgg tcaaacacca gcccatcaag
    14221 tcccgcctcc atcaggcggc ggcccagtcc tcgcgcccgc gccgaaggcg ccacgccgat
    14281 cgacagcacc tcagcttcat cggcagcaac acgcagcaac aggaagccga caatcctgcc
    14341 attgtccgcg acatgggcgc ggaccgtccg gtcggcgagc agcgattcga ccgtcagcgt
    14401 cggccaggga tgggaaaacg acaatgaatg cagttcggca atggcccttg catcgtccat
    14461 gcgggccggg cgcagagccg cctgacggcg acgggacagg ccgaaccagc gcatcaggcc
    14521 cgcgctatct ggccgtgccc ctgcggcacg gcacttgccg ccttcagata gagggggcgg
    14581 gcaggggcac aggcagggtc tgccgcagcc gcaaggcggg caacccagag gatatcgggc
    14641 gccggcccgg tctcgatcaa atccgcctcc agtccaagct tgcgggcatg atcatgtagc
    14701 gccggagcgc ctgtgccgac caggcggcag cgaccactgc gcagggtttc ggcagcctgt
    14761 tcgagcccaa gaaccagagg gccgagcgcc ttcttgccat ccagccagaa gctttcaaag
    14821 aagaccgcac catggcgggc atcaatggcg acagcggtca tcgaccgggc gccggacgca
    14881 atgaccggtg cagcataggc ggccagagtc gacacgccaa ccaccggaat cgccaaagcc
    14941 aggccaagcg aacgggcggc cgatacggca accctcaatc cagtgaagct gccaggcccg
    15001 actgtcgtgc cgatccggct caacgagccg aagccgccct ccaccccggc catcgcctgc
    15061 tcaatcatcg gcatcaaggc ctcggcatgg ccacgctgca tcggcaggct ggcgcgggcc
    15121 agtaccgttc cggacggata atcgaccacc gccaccgagg tagcgccgag cgcagtatca
    15181 agggccagga cacgcacccc gcgccgatcc ggtcagattg cctcaaccga ctgaacgtcg
    15241 ggcagaaagt gcttgagcag attctggatg ccatgcttga gcgtcgccgt ggaggaggga
    15301 cagccggagc aggcgccctt catggccaga tagacaacgc ccttttcgaa ccccctgaaa
    15361 gtgatatcgc caccatcgcc ggcaacggcc ggacggattc gcgtgtccag cagctccttg
    15421 atggtggcca cggtgtcggc gtccttggga tcatagaatt cgtcgccctc gtgctggggc
    15481 ggggcgacac cgtcgatgag cggggcgccg gaaagatagt gctccatgat cgcgccgaga
    15541 atggccggct tcaaatgctg ccattcccct gcggctttgg taaccgtgac aaaatccgag
    15601 ccgaaaaaca cgccggtgac cccgggaatg gaaaacagcc gcccggccaa gggagaagcc
    15661 gccgcatcgg ctgcggagcg aatgtcgagc gtgccctgat gcatgaccgc cttgccaggc
    15721 agaaacttca aggttgccgg gttgggcgtg acttcggtct gaatgaacat gatccctcgc
    15781 tgtctgccgt tcaaggcggc aaaagcctgc ttcaatgtag cgtctcctgg ctccagttca
    15841 aagccctgaa accaaacaga tcaccggctc accaacccga cgatgtcctt gacgtcgtcc
    15901 atcacgctgg aggcgatggc acgggcgcga tccccgccat tgcgcaggac ggcatcgatc
    15961 gaggccggat cggccagcca gcggcgcatc tcgccggcaa tcggggcaag cgaggacacg
    16021 gccagatcgg ccagcgccgc cttgaaggtg gaaaactgcc caccaccgta ttcgctgagc
    16081 accgcggccc gctcgcggcc cgacagcgcg gcaaagatgg tgaccagatt gtccgcctcg
    16141 ggtcggccgg ccagtccctc gatctcgccg ggaagcggtt cgggatcggt cttggccttg
    16201 cggatcttct gggcgatggc atcggcgtca tcgttgaggt tgatgcgcga atactccgac
    16261 ggatcggatt tcgacatttt cttggtgccg tcgcgcaggc tcatcacacg cggcgccgga
    16321 ccggcgatca agggttccgg caacgggaaa tagccctcgc catggccaag agcggcaatg
    16381 cgcgcggcaa aatcattgtt gaatttctgg gcaatatcgc gcgtgagttc gaggtgctgc
    16441 ttctgatcct cgccaaccgg cacatgggtg gcgcgataga ccaagatgtc ggcggccata
    16501 agattgggat aggcataaag gccaaccgac gcattctcgc gatccttgcc ggccttttcc
    16561 ttgaactggg tcatgcggtt gagccagccc aggcgggaaa cgcagttgaa cacccaggca
    16621 agctcggcat gttcagccac ccgactctgg ttgaagacaa tatggcgtcc aggatctata
    16681 ccggcagcca gaaaagccgc cgtgacctcg cgtgttgccc gaatcagatc ctcgggatcc
    16741 tgccagaccg tgatggcgtg aagatcgacg acgcaataga tgcaatcatg cgtatcctgc
    16801 agcgtcacaa accgacggat cgcgcccaga taatttccca gatgcaggtt gcccgtaggc
    16861 tggacgccgg aaaagacgcg cggcggaaca agtgccatat cgatcatcct tgtggagggg
    16921 ccgggttggt cgtctggagc gagggttgga acccgccgct ccgaaccgcc gcgctatgac
    16981 accactcaaa ggctgtttca agcgcctgtc cggccgaaag cacgtctgag gccaccaagg
    17041 tcggctatgc caaacagatg ggacgccacc gcgtgaacgc cgacgccaga caggcagacc
    17101 agggcaagcg cgcccgtccg caatgccagg gcccgatcag gcgccagcca gggttcgaac
    17161 aggaccagat tggcataggt cacggcaccg gtcagtgcgg ctgccacaac gatgagcggc
    17221 agagagcggg tcgcccgccc atccagacga aacacccccc gcatcttgag cccgataccg
    17281 aggagcagcg cattcaccca ggccgaggtg gcgacaccaa gcggtgcggc aacagggccc
    17341 aggcgcccga caagagcgag cgtaaaggca atgttggcaa ccaccccggc cagcgccgcc
    17401 agcagcggaa ggcgcagcga ttcgcgcgcc agaaaggccg gcagaaacac cttgaccagc
    17461 acaaagcccg gtagaccgag cgccaaggcc gagagcatgt cggccactgc cgcactgtcc
    17521 gaagcggtaa aggcgccgtg acgatagatg atgtcgatga tcgggcgggc cagaatgacc
    17581 agggcaagca tggcgggcaa aaccagcagc atcccgaatt cgagagcccg gttgctggca
    17641 tcgtcagcgc gcggatgatc gccatcacgg atggcgcggg aaacctcggg caacaggaca
    17701 actccgatcg ccgctgcgac aaaacccagc ggcaactgga aaatccgatc agcgtaatag
    17761 atccaagaaa ccgccgaagg cagcgccgaa gacaattgcg ccgccaccac catgttgaaa
    17821 tgcccggccc cggcaaggac aacggccggc agcaccatgg caatcatcag cctcacatcc
    17881 cggtcagcct cccagcgcag cggccagatc acaataccgg cgcggcggac accataggcg
    17941 acataggcaa gctggaggaa tccggcagcc agaaccgaga acaccaggac ccatccgacc
    18001 cgaacatcac cctgccagcc caaaacaaac agagcgagaa gaaccggaat cagcaggccg
    18061 ttgaggatca gtggcgccat ggcgaccagg gcatatcgcc cgacagcgtt cagcagcgag
    18121 gccagaaatg ccgaaacgat gacagccccg acaaagccaa aggcgatgcg tccgaacaga
    18181 accgcctggg cgaacttctc cggatcggca acaaatcccg gagcgatcac cttgatgatc
    18241 cctggcatcg acaattccgc caccaggaca agaccgaaag ccagcgccaa aatcgcggca
    18301 aaggctgccc tgacaaaatc cctggccgaa gcgtcccctt cggtccgtcg gcgggacaga
    18361 atcggcacga aggccgcgtt gaaggccccc tcgctgagga cccgccggaa cagattgggc
    18421 aacaggaagg cggcaaggta ggcatcggca acccggccgg agcccagcac tgctgcaatc
    18481 aggatatcgc gaacgaagcc ggtcaggcgc gatgcgagtg tcgccgaacc aaccagggta
    18541 taggaccgga ccagcgacat cccgaatgcg cggcgctaga gaatgaaccg gctgagatcg
    18601 gcattgcgcg ccagatcgcc aaggcttgcc tgcacctgag cgccgtcgat cacaaccttc
    18661 tgccccgact tgtcgggcgc cgaaaaggaa atctcgtcca atatgcgttc catcaccgtc
    18721 tgcaggcggc gcgcgccaat gttctcgatg gtcgcgttga cctcgaaggc tgcgtcggca
    18781 atggcatcca gcgcatcgtc ggtgaaggat agttcgacct gctcggtgcc caaaagcgcg
    18841 acatattgct tgaccagcga tgcctcggtt tccgtcagga tgcgacggaa atcgctgcgc
    18901 gccaacggtg tcagttcgac gcgaatcggc aggcgcccct gcaattcggg cagaagatcc
    18961 gacggtttgg agacgtgaaa tgcgcccgag gcgatgaaca ggatatggtc ggtcctgacc
    19021 gctccatgct tggtcgacac cgtcgtgccc tcgataagcg gcaggagatc acgctggaca
    19081 ccctcgcggc tgacatccgc gccaccgcga ttctcacgcg cgcagatctt gtcgatctcg
    19141 tcgagaaaga cgatgccgtt gttctccacc tcgcgcagag catcctgaac aatctggtca
    19201 tggtcgatca gcttgtcgct ttcctcctgc acaagcgggc tgtgggcctc cgccaccgtg
    19261 gtccggcgct tgcgcatggg cttgccgaac gccctgccca gcaactcgcc gagcgctgcc
    19321 tgtcccggct gggcgccggg catttcaagc atcgggatcg ccggggccgg tgaagagacc
    19381 tcgatgtcga tatccttgtc attgagttca ccggccctga ggcggcggcg gaaggaatcg
    19441 cgcgtgccgg ggctggccgt tgcgcccaca agagcgtcga tcacacgttc ctcggccgcc
    19501 agttccgcct tggccc
//
LOCUS       DAOMXG010000028        19151 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_1671_length_19151_cov_10.262620, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000028 DAOMXG010000000
VERSION     DAOMXG010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 19151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19151
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1671_length_19151_cov_10.262620"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /gene="odhB"
                     /locus_tag="PLQ11_03395"
     CDS             <1..725
                     /gene="odhB"
                     /locus_tag="PLQ11_03395"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HRK23979.1"
                     /translation="AASAPTDAAREERIKMTKLRQTIARRLKESQNTAAMLTTFNEVD
                     MGAVMALRNQYKDLFEKKHGVKLGFMSFFTKAVVQALKELPGVNAEIDGTDIIYKNYY
                     HVGIAVGTDKGLVVPVLRDADRLGLAGIEKSIADFGKRARDGQLKMEEMSGGTFTITN
                     GGIYGSLMSTPILNAPQSGILGMHKIQDRPMVVGGKIEIRPMMYLALSYDHRIVDGKE
                     AVTFLVRVKESLEDPARLVMDL"
     gene            831..1574
                     /locus_tag="PLQ11_03400"
     CDS             831..1574
                     /locus_tag="PLQ11_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRK23980.1"
                     /translation="MTRVAIVTGASRGIGRACAIGLAEDGFDVVVNYAGDRTAADEVV
                     AVIAATGRRAVAVQGDVSVEADIEKIFVAADALGTLAALVNNAGVVDIAQPVVEMSTA
                     RLRRMFDINVMGTIWCAQAAIRRMSTRLGGKGGAIVNMSSIAPRLGAPGQYVDYAAAK
                     GAIDALTLGLGRELANEGIRVNGVQPGLIETDIHASGGIPDRIEQLKATIPIKRGGTA
                     KEVAEAVRWLVSDKASYAVGSLITVSGGR"
     gene            1601..3001
                     /gene="lpdA"
                     /locus_tag="PLQ11_03405"
     CDS             1601..3001
                     /gene="lpdA"
                     /locus_tag="PLQ11_03405"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HRK23981.1"
                     /translation="MSYDLIVIGTGPGGYVCAIRAAQLGLKTAVVEKWKTHGGTCLNV
                     GCIPSKALLHASEVFDEAGHGMAALGIEIGKPKLNLAAMMKHKQETIDANVNGVGFLL
                     KKNKVDVHFGLGTILAPGKVQVTANDGKTSVIEAKAICIATGSEVAGLPGVEIDEKQI
                     VSSTGALGLGKVPGKMVVIGAGVIGLELGSVWRRLGAEVEVIEYLDRILPGMDLETCK
                     QFQRILEKQGISFRLGAKVTGAETIKGGVKLKVEPAAGGEKTEIGADVVLVAIGRKPY
                     TTGLGLEAVGVKLDNRGRVVTDHHYATSVPGIYAIGDVITGPMLAHKAEDEGVALAEM
                     LAGKAGHVNYDVIPGVVYTFPEIASVGKTEEELKAAGIAYNVGKFPFMANGRARANRT
                     TDGFVKVLADKATDRVLGVHILGAGAGEMIHEAAVLMEFGGSSEDLARTCHAHPTMSE
                     AVKEAAMAVEKRQIHM"
     gene            complement(3204..3770)
                     /locus_tag="PLQ11_03410"
     CDS             complement(3204..3770)
                     /locus_tag="PLQ11_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HRK23982.1"
                     /translation="MSGMPEAHEGNKKIALLIAVLALFLAFAEAGNSNNENLALESNI
                     EASNLWAFFQAKTIRSTVLKTAAEQMETDLARVSDPAAQEAMKKRIDSWNKTIARYDS
                     EPETSEGRKELAARAKDKEAARALASQKKEIFEVAAKLFQIAIIIASAVIITGIGWLG
                     LVATALGLGGAVLMGFALWAPQAIHLPF"
     gene            complement(3820..4785)
                     /locus_tag="PLQ11_03415"
     CDS             complement(3820..4785)
                     /locus_tag="PLQ11_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HRK23983.1"
                     /translation="MLSAAPHAATMDIPTALRQWLGHLASERRLSANTLDAYGRDMRQ
                     FLVFIEERGGRPAKVDDLAGLTPADVRAFLAARRSSGAESRTLMRQLAGCRSFLRFCE
                     REGIAKASAFSAVRGPRVARGLPKPIAARAARELAAPDIRMGEAREPWIFDRDAAVLA
                     LLYGCGLRISEALSLARKNAPVSGVDALTITGKGGRMRQVPVIGPVQTAVERYLASCP
                     WRLAADGPLFVGARGGALSPRIIQLAVERLRGALGLAETATPHALRHSFATHLLGRGG
                     DLRTIQELLGHASLSTTQIYTEVETARLIASVRSAHPRARMSATS"
     gene            4830..7043
                     /locus_tag="PLQ11_03420"
     CDS             4830..7043
                     /locus_tag="PLQ11_03420"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HRK23984.1"
                     /translation="MDPTPCPARTERPDIADVLVPVAVDTAYSYRIPAGLDLVEGDTV
                     VVPLGTRQSIGVVWSLRQGPGGANLKQVAGRYDLPPMRPAMRAFVEWIARWTLSPRGM
                     VARMALRDPDQVGPERPKLGVRFTGLHPERMTPARRRVLTAAENGMVWRKSALAEAAS
                     VSSGVIDTLVDEGALEVTHIPADPVAPLPDADHAAMALSQDQADAAANLGAMVQSGAF
                     AVALIEGVTGSGKTEVYFEAIAAALRLGRQALVLMPEIALTADFMARFEARFGVRPAE
                     WHSGVSASRRDRLWHAVATGQARIVVGARSALMLPFADLGLIVVDEEHEAAYKQGDGV
                     RYNARDMAVVRGRIEGAAVVLASATPSVESRVNAESGRYRHLRLPDRFGGAILPELAA
                     IDLRKSPLPPGRWIAAPLLEAARTTLAAGEQVLFFLNRRGFAPLTLCNRCGHRWQCPH
                     CSAWLVEHRFRRALVCHHCGHTERRPPTCVSCGAENELRAVGPGVERLAEEVAELFPG
                     ERSIVLSSDFPGGTERLRQELAAIAAGEYRIVIGTQLVAKGHNFPSLTLAGVIDADIG
                     LANADPRAAERTFQMLTQVTGRTGRHQRPGRGLIQTTQPDHPVLRAILTHDREGFYRE
                     EIRMRQLGGLPPFGRLAALMVSARDRAVADEAARRLAQAGLALSAEPRFASARLLGPA
                     EPPLALLRGRHRMRLIIKTARGDDLQGFLRAMIANAGPMRSGARLDVDVEPLSFL"
     gene            7233..7796
                     /locus_tag="PLQ11_03425"
     CDS             7233..7796
                     /locus_tag="PLQ11_03425"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HRK23985.1"
                     /translation="MADQSPIVSGIAGRYASALFELAEEQKATDQVGKGLQAFGALLA
                     GSDDLQRLVKNPVFTAEEQIKAVSAVLDKAKIGGLAANFIRLVASKRRLFAIDGMIRG
                     FASLVDLKNGVVTAEVTVPAPLSAKNREAVLEAIKGVTGGNSIALNEKVDPAILGGLI
                     VKVGSKMVDASLKTKLNSIKLAMKEVG"
     gene            7796..9328
                     /gene="atpA"
                     /locus_tag="PLQ11_03430"
     CDS             7796..9328
                     /gene="atpA"
                     /locus_tag="PLQ11_03430"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HRK23986.1"
                     /translation="MDIRAAEISAILKDQIKNFGSAAEVTEVGQVLSVGDGIARVYGL
                     DKVQAGEMVEFENGIRGMALNLESDNVGVVIFGSDRDIKEGQTVKRTGAIVDVPVGKE
                     LLGRVVDALGNPIDGKGPIKAKARSRVDVKAPGIIPRKSVHEPMATGLKAVDALIPIG
                     RGQRELIIGDRQTGKTAIALDTILNQKPLNAAGAPESQKLYCVYVAVGQKRSTVVQFA
                     KVLEEQGALDYSIIVAATASDPAPMQFLAPFSGCAMGEFFRDNGMHAVIIYDDLSKQA
                     VAYRQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKLNETNKAGSLTALPVIETQAND
                     VSAYIPTNVISITDGQIFLETDLFYQGIRPAVNVGLSVSRVGSSAQTKAMKKVAGKIK
                     GELAQYREMAAFAQFGSDLDAVTQRLLNRGARLTELLKQAQFSPLKMEEQVCVIYSGV
                     NGYLDGLPVNRIKAYEDGLLALLRSKHEGLLNSIRDSKDLSAADEATLKSVVEGFTKS
                     FA"
     gene            9392..10270
                     /locus_tag="PLQ11_03435"
     CDS             9392..10270
                     /locus_tag="PLQ11_03435"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HRK23987.1"
                     /translation="MPSLKDLRNRIASVKATQKITKAMQMVAAAKLRRAQGAAEQARP
                     YAARMGDVLGNLAAGSTGGADTPRLLAGTGSDKVHLLIVCTAERGLCGAFNSSIVRLA
                     REHINQLLAEGKTVKIFTVGKKGNEQLRRQFGAHIIETVEFRGVKQLGYDHAESVGRR
                     VLEMFDKGEFDVATLFYSQFKSVIAQIPTAQRLIPAEVQAGGAASGVKAAYEYEPEEG
                     VILSSLLPKNVSVQIFRALLENAASEQGARMSAMDNATRNAGDMIRKQTQKYNRSRQA
                     MITKELIEIISGAEAL"
     gene            10294..11766
                     /gene="atpD"
                     /locus_tag="PLQ11_03440"
     CDS             10294..11766
                     /gene="atpD"
                     /locus_tag="PLQ11_03440"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018430148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRK23988.1"
                     /translation="MAKAAAKASTKAAAPGGATGRITQVIGAVVDVQFDGALPEILNA
                     LETTNGGNRLVLEVAQHLGENSVRCIAMDTSEGLVRGQEVINTGAPIMVPVGDECLGR
                     IINVIGEPVDEAGPVKTKTRRAIHQDAPSYADQSTEAQILVTGIKVVDLLAPYAKGGK
                     IGLFGGAGVGKTVLIMELINNVAKAHGGYSVFAGVGERTREGNDLYHEMIEGGVNKKP
                     ENGSTAGSKAALVYGQMNEPPGARARVALTGLTVAEHFRDQGQDVLFFVDNIFRFTQA
                     GSEVSALLGRIPSAVGYQPTLATDMGAMQERITTTTKGSITSVQAIYVPADDLTDPAP
                     ATSFAHLDATTVLSRSIAEKGIYPAVDPLDSTSRMLSPLVVGAEHYEVARKVQSTLQR
                     YKSLQDIIAILGMDELSEEDKLVVARARKIERFLSQPFHVAEVFTGSPGKLVALEDTI
                     KGFKGLVEGKYDHLPEAAFYMVGTIEEAVEKAQKLAAAAA"
     gene            11788..12189
                     /locus_tag="PLQ11_03445"
     CDS             11788..12189
                     /locus_tag="PLQ11_03445"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HRK23989.1"
                     /translation="MASFNFELVSPERILFSGPVDQVVVPGEDGDMTILANHAPVMTT
                     LRSGIVAVSADKGAARRLYVRGGFADVTASGLTILAEVAIPVEEISLDKLAGDMKNAE
                     DDLRDAKTDETRKAAEMRLAGLRAMQAALRN"
     gene            complement(12267..14120)
                     /locus_tag="PLQ11_03450"
     CDS             complement(12267..14120)
                     /locus_tag="PLQ11_03450"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRK23990.1"
                     /translation="MRLRAMLLAGAALMPSLADAQSFSRIITFGDSLSDNGNLFSLAG
                     TPPAPYFQGRFSNGPVWAELLNGPMTRAGGSILGIPPLPAALSPTANLNFAFGGARTD
                     SAVAMPPGTVTQINNYIGLGGRFTTTDLVTLWAGANDIFQSAPTQAAVTVAATSAATN
                     VGNQVRTLAGAGAPIILVMNLPNLGAAPNYLAQGPLGVQLGSYATATFNGAWNTAVSA
                     AAAANPATNVVQVPIDALLTAAIANPGAFGLSNVTNQCILTPACVTGNAATQATFLFW
                     DGVHPTATGHQMIARFAQEYLFAPTRAMAMGGLGDVGFWSRRQAMGDMMDRVQVAAPG
                     EGKPEYFISAIGEQGSRNSSLVTAGFTGNALLSTDAGARYAAGGLRFGVFHKFNPAWT
                     AGIGLSATVGDAKSGTVSFSPVAFAADLAARWSQGPTFVNLGLGATVTNYREIERKTL
                     VGNLTNSGQTTGVGYSAVAEAGYRWNMGSYAVTPKARLSYVSASVNKFNESGVVAPIA
                     FAGRTVQGLAAGAELRVDATVAAGTRAHALIGYEEFLYSSAGKLGGRLIANTAMSFSA
                     NLKDPVGAGLLLGLGLDAQIGGWAASATYRATVGQKSQVTHRGQITVGASF"
     gene            14337..15956
                     /locus_tag="PLQ11_03455"
     CDS             14337..15956
                     /locus_tag="PLQ11_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014765686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRK23991.1"
                     /translation="MLLPDIRDYDRLYRSFRWDIPARFNIATSVCDVWAERDGDRPAI
                     IEVPVSGDVRITSYRQMREQSNRLANALVRHGIAAGDRVAILLPQSAAVPVAHLAVYR
                     MGAIALPLAVLFGVDALQYRLTNAGARVLITDAAGVGKIAAIRGALPGLELILSVDGD
                     DGAAIGLDRFVAGAGADFPTAPTGPDDPALMIYTSGTTGQPKGALHAHRVLLGHMPGV
                     QWPHEFMPQPGDRVWTPADWAWIGGLMNVLMTSLACGVPAVAHVFEKFDPEAAFAFMA
                     QHQVRNTFIAPTALRMLRTVARPRERFGISLRTLASGGETLGEEVYAWGRQELSLTIN
                     EFYGQTECNLIAASCAAGGVSRSGSLGKAVPGHVVGIIREDGTPCAVGEIGIIAARRP
                     DPAMFLEYWGQPEATRAKFIGDWMKTGDQGRFDEDGYIFFVGRDDDVITSSGYRIGPG
                     EIEDCLISHPKVAIAAVVGKPDPVRTEIVKAFVVLKPGIAGDETLRRDIQSYVRTRLS
                     AHEYPREIEFVDSLPLTTTGKIIRRLLREQR"
     gene            complement(16096..17352)
                     /locus_tag="PLQ11_03460"
     CDS             complement(16096..17352)
                     /locus_tag="PLQ11_03460"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRK23992.1"
                     /translation="MQKRIRMQVFTDTRLPNLVGPALYQGEVPASLLHATRPSDAAIV
                     VEELAAGDLARIEPDLRWLEARASEANPFYGADFLLAASRHFRRTARPLCLAAWRPQS
                     GHGGQRLVGLWPLAEVHKLRMGETIGGWTHPFVVLSQPLVDRSECDAVVQAFIDRMLS
                     GKDAALKLSQIAADGPIMVAIQRAVAKAGSRITMLARYERAVFATAKSIPAQLETAGK
                     ELRRQSRRLAEQGQLSFGVVDGYADLKAGIEDFLALEASGWKGRAGTSLLNHAETANF
                     ARAMLWSAARRGRLKVARLELDGKMIAGGLVLCAGTTGFLWKIAFDETHARFSPGVQM
                     IHKLSNWAVETQAFTMIDSCAHAGHRMIEAVWTGRRELCDLMIDLAPLSPVRFRMAVF
                     GEAARRRVIGSLKGLRQRVRVFARAA"
     gene            complement(17429..18088)
                     /locus_tag="PLQ11_03465"
     CDS             complement(17429..18088)
                     /locus_tag="PLQ11_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23993.1"
                     /translation="MRPQPVPVLVVSGAELSARGDVLEAVLASADDGATLLLASGALG
                     QPRRAEAMALPDGAADRLSGACLCCTGRDDLVLALEDVLRSVDNHRMRPIRRLVIAAD
                     RAADPAALAGAIRGHPYLSLRFAPVAVLAVAGSDDLTEGQAAFDRRRLAVADWLVVDP
                     AVDSQFAETNQEVPRLLPNALSGALTAFWSGAGPAAGSRGQHHRRALLRQALRPYDRR
                     S"
     gene            complement(18097..19020)
                     /locus_tag="PLQ11_03470"
     CDS             complement(18097..19020)
                     /locus_tag="PLQ11_03470"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRK23994.1"
                     /translation="MTGLGDAGAAVRRATLVGSALALVGATAYGINIVGARLCAQLGI
                     SGSDIVVYRAFLFLPLLAGLALWRGQRLALSPGNRAPVFRFAVFSVATALFYMSSLAY
                     LPVPIAVTIFYTYPLIVILLSPYVDRVPLSLRRWVVALVAFAGVLLAVGPEAHRLDPR
                     GVVLALFGSAACAGMFISAARVETDSSVTFFWCQICALPLGLGFAHVMGGLSEPAVLM
                     AGLAPLLVNVAGYFIGFLLQIMAAARISPATAGLLFLFEPVVAILAAALVLSETLTLA
                     QTVGIALVVGALAFDLLPHLRRSEPITPFEP"
BASE COUNT         3519 a         6343 c         6093 g         3196 t
ORIGIN      
        1 gtgcggcctc ggccccgacc gatgcggccc gcgaggagcg gatcaagatg accaagctgc
       61 gccagacgat cgcgcggcgc ctcaaggaaa gccagaacac cgccgccatg ctgaccacct
      121 tcaacgaggt cgacatgggc gcggtgatgg cactccgcaa ccagtacaag gacctgttcg
      181 agaaaaagca tggcgtgaag ctcggcttca tgagcttctt caccaaggcc gtggttcagg
      241 cgctgaagga actgccgggc gtcaacgccg agatcgacgg caccgatatc atctacaaga
      301 actattatca cgtcggcatt gcggtcggca ccgacaaggg cctcgttgtg ccggtgctgc
      361 gcgacgcgga ccggctcggc cttgccggca tcgagaagtc gatcgccgat ttcggcaagc
      421 gcgcccgcga cggccagctc aagatggagg agatgtcggg cggcaccttc accatcacca
      481 acggcggcat ctacggttcc ttgatgtcga caccgatcct caacgcgcca cagtcgggta
      541 ttctcggcat gcacaagatt caggatcgcc cgatggtggt cggcggcaag atcgagatcc
      601 gcccgatgat gtatctggcg ctctcctacg atcaccgtat cgtcgacggc aaggaagcgg
      661 tgaccttcct cgtgcgcgtc aaggaaagcc ttgaggatcc ggcgcgtctc gtgatggatc
      721 tgtgaccggc caaaaccctt gttcgggact tgtcgaaaca tccagacgaa ggcctcgccc
      781 agcatggatc ggcaggctcg tcagggcggc agcgctgtga ggaggacgcc gtgacccgcg
      841 tcgccatcgt taccggagcc tcgcgcggca tcggccgcgc ctgcgccatc ggcctggccg
      901 aggacgggtt cgatgtcgtc gtcaactacg ccggcgacag gacagcggcg gacgaggtgg
      961 tcgcggtgat cgccgcgacg ggccgcaggg cggtcgccgt tcagggcgat gtctcggtcg
     1021 aggcggatat cgagaaaatc ttcgtcgctg ccgacgcgct cggaacgctg gccgcgctgg
     1081 tcaacaacgc cggcgtggtc gatatcgccc aaccggtcgt cgagatgtcg actgcgcggc
     1141 tcaggcggat gttcgacatc aacgtgatgg gcacgatctg gtgcgcacaa gccgcgatcc
     1201 gccgcatgtc gacccgcctc ggcggcaagg gcggggccat cgtcaacatg tcctcgattg
     1261 ccccgcggct cggcgcgccc gggcagtatg tcgattatgc cgccgccaaa ggggcgattg
     1321 acgctctcac cctcggtctt ggccgcgaac tggccaatga gggaatccgc gtcaacggcg
     1381 tccagcccgg cctgatcgag accgacattc acgcctcggg cggcatcccc gaccggattg
     1441 aacaactcaa ggcaaccatc ccgatcaagc gcggcggcac ggcaaaggaa gttgccgagg
     1501 ccgtgcgctg gcttgtttcc gacaaggcgt cctacgccgt cggctcgctc atcaccgttt
     1561 ccggcggccg ttgagccgcc tgccagaaag ggaaatcccc atgtcctacg atctcatcgt
     1621 catcggcacc ggccccggcg gttatgtctg cgccatccgc gcggcccagc tcgggctcaa
     1681 gacggcggtt gtcgagaaat ggaagaccca cggcggcacc tgcctcaacg ttggctgcat
     1741 cccctcgaaa gcgctgctgc atgcctcgga ggtcttcgac gaggccgggc acggcatggc
     1801 ggcgctcggc atcgagatcg gcaagccgaa gctgaatctg gccgcgatga tgaagcacaa
     1861 gcaggagacc atcgacgcca acgtcaacgg cgtcggcttc ctcttgaaga agaacaaggt
     1921 tgatgtgcac tttggcctcg gcaccatcct tgcgccgggc aaggttcagg tgacagccaa
     1981 cgatggcaag acttcggtga tcgaggccaa ggcgatctgc atcgccaccg gctccgaggt
     2041 tgccgggctg ccgggcgtcg agatcgatga aaagcagatt gtctcttcca ccggcgcgct
     2101 cggcctcggc aaggtgccgg gcaagatggt ggtgatcggc gccggcgtca tcggccttga
     2161 actcggttcg gtgtggcggc gcctcggcgc ggaagtcgag gtgatcgaat atctcgatcg
     2221 catcctgccc ggcatggatc tggagacctg caagcagttc cagcgcatcc tcgaaaagca
     2281 gggcatcagc ttccggctcg gcgccaaggt gaccggcgcc gagacgatca agggcggcgt
     2341 caagctgaag gtggaaccgg cggctggcgg cgaaaagacc gagatcggcg ccgatgtggt
     2401 gctcgtcgcc atcggccgca agccctatac gaccggcctt gggctggaag cggtgggcgt
     2461 caagctcgac aaccgcgggc gcgtcgtcac cgaccatcac tatgccacca gcgtgccggg
     2521 catctatgcc atcggcgatg tcatcaccgg cccgatgctg gcccacaagg ccgaggatga
     2581 aggcgtagcg ctggcggaaa tgctggccgg caaggcagga catgtaaatt acgatgtcat
     2641 ccccggtgtc gtctacacgt tcccggagat cgcctcggtc ggcaagaccg aggaggaact
     2701 gaaggcggcg ggcatcgcct ataacgtcgg caagttcccg ttcatggcca atggccgcgc
     2761 ccgcgccaac cgcaccacgg acggcttcgt caaggtgctg gccgacaagg cgaccgaccg
     2821 cgtgcttggc gtccatatcc tcggcgccgg cgccggcgag atgatccacg aggcggcggt
     2881 actgatggaa ttcggcggct cgtccgagga tctcgcccgc acctgccatg cccatccgac
     2941 gatgtcggag gccgtcaagg aagcggcgat ggcggtcgaa aaacgccaga tccacatgta
     3001 atatcagtgg ccctcggact tgacctatcg gtcaatctcg ggccgttccc tcgcaacggg
     3061 cctttggccc tgctcggcgt cgcattcgct cctaggacga cggcctccgg agtaaaaagc
     3121 cggagccggt ccacgcaccg cgcgccttcg atcctgcctg gccgcatgca ctcaggcgca
     3181 gcggccggcc gggatgcggc ctctcagaac ggcaggtgga tggcctgcgg cgcccacaag
     3241 gcaaatccca tcagcaccgc cccgcccaga ccaagtgccg tcgcgacaag cccgagccag
     3301 ccgatacctg tgatgatcac cgctgaagcg atgatgatgg cgatctggaa cagcttggcc
     3361 gcaacctcga agatctcctt cttctggctg gcaagagccc gtgccgcttc cttgtccttg
     3421 gcccgggcgg caagttcctt gcgcccctcg ctggtttccg gctcggaatc gtagcgggcg
     3481 atcgtcttgt tccaggaatc gatgcgcttc ttcatagcct cctgcgcggc tggatcggac
     3541 acgcgggcaa ggtcggtttc catctgttcg gcagccgttt tcagaacggt cgagcggatg
     3601 gtcttggcct ggaagaacgc ccagagattg gacgcctcga tgttggactc cagggcgaga
     3661 ttttcgttgt tgctgttgcc ggcttcggca aaggcaagaa aaagcgccag caccgcgatc
     3721 agcagcgcga tcttcttgtt gccctcgtgg gcttcaggca tgccagacat cggaatctcc
     3781 cgggaaagga gcccgctgtc gctgattcga tgcgcaatct caagatgttg cagacattct
     3841 ggcgcgcggg tgggctgacc ggactgaagc gatcagccgg gccgtttcaa cctcggtgta
     3901 gatctgtgtg gtcgacaagg aggcatggcc gagcagttcc tggatggttc tcaggtcccc
     3961 gccccggccc agcagatggg tggcaaagga atggcgcagg gcatgcggcg tcgccgtttc
     4021 ggcaagcccg agcgcgccgc gcagccgctc gaccgccagc tggatgatgc ggggggacag
     4081 agcccctccc ctggcgccga cgaacaatgg cccgtcagcc gccagccgcc agggacagct
     4141 ggcaagatag cgctccacgg cggtctgcac cggcccgatc accggcacct ggcgcatgcg
     4201 cccgcccttt ccggtaatgg tcagggcatc gaccccgctt acaggcgcgt tcttgcgggc
     4261 cagcgacagc gcttcggaaa tgcgcagacc gcagccgtaa agcagcgcaa gcaccgccgc
     4321 atcgcgatcg aagatccacg gttcgcgcgc ctcgcccatg cggatgtccg gcgcggcaag
     4381 ctcccgtgcg gcgcgggcag cgatcggctt gggcaagcca cgcgcaaccc tgggcccgcg
     4441 cactgcggaa aatgccgatg ccttggcgat cccctcccgt tcacagaacc gcaggaacga
     4501 ccggcacccg gccagctgcc gcatcagggt gcggctttcg gctccggatg aacgccgtgc
     4561 cgccagaaac gcccgcacgt ccgcaggggt caggccggca aggtcgtcga ccttggccgg
     4621 gcgcccaccc cgctcctcga tgaagacgag aaactgccgc atatcgcggc cataagcatc
     4681 caacgtgttg gccgacaggc ggcgttcgga agccagatgg ccaagccact ggcgcagggc
     4741 tgtaggaata tccatcgtgg cagcatgcgg ggcggcgctt aacatgcggt ttctggaacg
     4801 ggcgggcgaa tcggtgctat cgctcaggca tggacccgac gccctgccct gcccgcaccg
     4861 agcgccccga catcgccgat gtgctggtgc cggttgcggt cgatacggcc tattcctacc
     4921 gcatcccggc agggctggac cttgtggaag gcgataccgt ggtcgtgccg ctcggcacac
     4981 gccagagcat cggtgtggtc tggtcgctcc gccaggggcc cggcggcgcc aatctcaagc
     5041 aggttgccgg tcgctatgac ctgccaccca tgcgcccggc gatgagggcg tttgtcgaat
     5101 ggatcgcccg ctggaccctg agtccgcgcg gcatggtggc ccgcatggcg ctccgcgacc
     5161 ccgatcaggt tggcccggag aggccgaaac tcggggtgcg tttcaccggt ctgcaccccg
     5221 agcggatgac ccctgcccgc cggcgcgtgc tgaccgccgc cgagaacggc atggtctggc
     5281 gcaaatcggc attggcggag gctgcgagcg tctcctccgg cgtcatcgac acgctggtcg
     5341 acgaaggcgc gcttgaggtg acccatattc ctgccgatcc tgtcgctccg ctgcccgatg
     5401 ccgatcatgc agccatggcc ctgtctcagg atcaggctga cgccgcagcc aacctcggcg
     5461 cgatggtgca gtcgggtgcc tttgcggtgg ccctgatcga gggcgtcact ggctcgggca
     5521 agaccgaagt atattttgaa gccattgctg cggccctgcg ccttgggcgc caggcgctgg
     5581 tgctgatgcc ggaaatcgcc ctcactgccg atttcatggc gcgttttgaa gcacgcttcg
     5641 gtgtccgtcc ggccgagtgg cattccggcg tcagcgcgtc acgacgcgac cggctgtggc
     5701 acgcggtcgc gaccgggcag gctcgcattg ttgtcggcgc gcgttcggcg ctgatgctgc
     5761 cttttgccga tctgggcctg atcgtggtcg atgaggagca tgaagccgcc tacaaacagg
     5821 gcgacggcgt gcgctacaat gcccgcgaca tggcggtggt gcgcggccgg atcgaaggcg
     5881 cggcggtcgt gcttgcctcg gccacgccct ctgtcgaaag ccgcgtcaat gccgaatcgg
     5941 ggcgctatcg tcacctgcgg ctgccggatc ggttcggcgg cgcgatcctg ccggaacttg
     6001 ccgccatcga cctgcgaaag tcgccactcc cccccggccg ctggatcgcg gcgccgctgc
     6061 tggaggcggc acggacgaca ttggcagcgg gcgagcaggt cctgttcttc ctcaaccggc
     6121 gtggctttgc cccactcacc ctgtgcaacc gctgtggcca tcgctggcag tgcccgcatt
     6181 gttcggcctg gctggtcgag caccggttcc gccgggccct tgtctgccac cattgcggac
     6241 ataccgaacg ccgaccgcca acctgcgtct cctgcggcgc cgagaacgaa ttgcgcgccg
     6301 tcggccccgg cgtcgaacgg ctggccgagg aagtggcgga actgtttccc ggcgaacgca
     6361 gcatcgtcct gtcgtccgat tttcccggcg gaaccgaacg gttgcggcag gagctggcag
     6421 cgattgccgc cggcgaatac cggatcgtca tcggcaccca gcttgtcgcc aagggtcaca
     6481 actttccgtc cctgacactg gccggcgtca tcgacgccga tatcggcctt gccaatgccg
     6541 atccgagagc ggccgagcgc acgttccaga tgctgacgca ggtcaccggc cgcaccgggc
     6601 gtcaccagcg gccgggacgc ggtttgatcc agaccaccca gcccgatcac ccggtcctgc
     6661 gggcgatttt gacgcatgac cgcgagggat tctatcgtga ggaaatccgc atgcgtcagc
     6721 tgggcggcct gccgccgttc ggccggctcg ccgcattgat ggtgagtgcc cgcgatcggg
     6781 cggtggccga cgaagctgcc cggcggctgg cgcaagccgg actggcattg tctgccgagc
     6841 cccgctttgc ctctgcccgt ctgctcgggc cggccgaacc gccgctggcg ctgctgcgcg
     6901 gccgccaccg catgcgtctg atcatcaaga cggcacgcgg cgacgatctg cagggttttc
     6961 tgcgcgccat gatcgccaac gccgggccaa tgcggtctgg cgcccgcctc gacgtcgatg
     7021 tcgagccgct cagtttcctc tgaccctagc cttttcgcca agacggaaag gggcgggaaa
     7081 gggttgcaac ccctcaagtt tgatgttagc gaccgggcac gtttcagggg ccgggccagc
     7141 aaatgcgggc acgcccttgt ccgatcccac cctgagcacg ttggcctggc ctctccggtc
     7201 cgggttgcga tgcgcgaaaa agagagagca tcgtggcgga tcagagcccg atcgtttccg
     7261 gaattgcggg gcgctatgcc tccgcattgt ttgagcttgc cgaggagcag aaggccaccg
     7321 accaggtcgg caagggcctt caggcgtttg gcgcgctgct tgccggcagt gacgacctgc
     7381 agcggctggt caaaaatccg gttttcaccg ccgaggagca gatcaaggcc gtctcggctg
     7441 tgctcgacaa ggccaagatc ggcggccttg ctgccaattt catcaggttg gtggcttcca
     7501 agcgccggct gtttgccatt gacggcatga tccgcgggtt tgcctcgctg gtcgacctga
     7561 agaacggggt cgtgaccgcc gaggtcacgg ttcccgcgcc gctgtcggcc aagaaccgcg
     7621 aggctgtgct ggaggcgatc aagggcgtca ccggcggcaa cagcatcgcc ctcaatgaaa
     7681 aggttgatcc tgccattctc ggcggcctga tcgtcaaggt cggctccaag atggtggacg
     7741 cttcgctcaa gaccaaactc aattcgatca aactcgccat gaaagaggtc ggctgatgga
     7801 catccgcgcc gctgagattt ccgcgatcct caaggatcag atcaagaact tcggctccgc
     7861 cgccgaggtc accgaagtag gtcaggtgct gtcggtgggc gacggtatcg cccgcgtcta
     7921 cggcctcgac aaggttcagg ccggtgagat ggtcgagttc gagaacggca tccgcgggat
     7981 ggcgctcaac ctcgaaagcg acaatgtcgg cgtcgtgatc ttcggttccg accgggacat
     8041 caaggaaggc cagaccgtca agcggaccgg cgccatcgtg gacgttccgg tcggcaagga
     8101 actgctcggc cgcgtcgtcg acgcgctcgg caatccgatc gacggcaagg gcccgatcaa
     8161 ggccaaggcg cgctcccgcg tcgacgtcaa ggcccccggc atcattccgc gcaagtcggt
     8221 gcatgagccg atggcgaccg gccttaaggc ggtcgacgcg ctgatcccga tcggccgcgg
     8281 ccagcgcgag ctgatcatcg gcgaccgcca gaccggaaag accgcgattg cgctcgacac
     8341 catcctcaac cagaagccgc tgaatgccgc cggcgctccc gagagccaga agctctactg
     8401 cgtctatgtc gcggtcggtc agaagcgctc caccgtggtg cagtttgcca aggtgctcga
     8461 ggagcagggc gcgctcgact attcgatcat cgtggccgcc acggcgtccg atccggcgcc
     8521 gatgcagttc ctggcgccgt tctccggctg cgccatgggc gagttcttcc gtgacaacgg
     8581 catgcatgcg gtcatcatct acgacgacct gtccaagcag gccgtcgcct accgccagat
     8641 gtcgctgctg ctgcgccgcc cgccgggtcg cgaagcctac cccggcgacg tgttctatct
     8701 gcactcccgc ctgctcgagc gcgccgccaa gctgaacgaa accaacaagg ccggctcgct
     8761 gacggccctt ccggtgatcg agacgcaggc caacgacgtc tccgcctaca tcccgaccaa
     8821 cgtgatttcg atcactgacg ggcagatctt ccttgagacg gatctgttct atcagggcat
     8881 tcgcccggcg gtgaacgtcg gtctgtcggt gtcgcgcgtc ggctcctccg cccagaccaa
     8941 ggcgatgaag aaggtcgccg gcaagatcaa gggcgagttg gcccagtatc gcgagatggc
     9001 ggcctttgcc cagttcggtt ccgatctcga cgcggtgacc cagcgcctgc tcaaccgcgg
     9061 cgcccgcctg accgagcttc tcaagcaggc gcagttctcg ccgctgaaga tggaagagca
     9121 ggtctgcgtc atctactccg gcgtcaatgg ctatctcgac ggcctgccgg tcaaccggat
     9181 caaggcttat gaggatgggc tgctggccct gctgcggtcc aagcatgaag ggctgctcaa
     9241 ttcgatccgc gacagcaagg acctttcggc cgctgacgag gcgacgctca agagcgtcgt
     9301 tgaaggcttc accaagagct tcgcctgaga ctgaactggc cggccggagg tctccggccg
     9361 gttccggacg cacgctgacg aggaacgtcg aatgccttcc ctgaaggacc tacgcaaccg
     9421 catcgcctcg gtgaaggcga cgcagaagat caccaaggcc atgcagatgg tggctgcggc
     9481 gaagctgcgc cgtgcccagg gcgccgctga acaggctcgc ccctacgccg cccgcatggg
     9541 cgatgtgctc ggcaatcttg ccgccggttc gacgggcggg gcggacacgc cgcgcctgct
     9601 tgccggaacc ggcagcgaca aggtgcacct gctcatcgtc tgcaccgccg agcgtggcct
     9661 gtgcggcgcg ttcaactcct cgatcgtgcg tctggcgcgc gagcacatca accagctgct
     9721 tgccgaaggc aagacggtga agatcttcac cgttggcaag aagggcaacg aacagctgcg
     9781 ccgccagttc ggcgctcata tcatcgagac ggtcgagttc cgcggtgtca agcaactcgg
     9841 ctacgatcat gccgagagcg tgggtcgccg cgtgctcgag atgttcgaca agggcgagtt
     9901 cgatgtcgcc acgctgttct attcgcagtt caagtcggtc atcgcccaga ttccgaccgc
     9961 ccagcggctg atcccggccg aggtgcaggc gggcggtgcc gcgtcgggcg tcaaggccgc
    10021 ctatgaatac gagcccgagg aaggggtcat tctctcctcg ttgctgccca agaacgtctc
    10081 ggtgcagatt ttccgggcgc ttctggaaaa tgccgcgtcc gaacagggcg cgcgcatgag
    10141 cgccatggac aacgccacgc gcaatgccgg cgacatgatc agaaagcaga cgcagaagta
    10201 taaccgctcg cgtcaggcga tgatcaccaa ggaacttatc gaaatcatct cgggcgctga
    10261 agcgctctga ccacaggata agaggtcaca accatggcga aagcagcagc aaaggcgtcc
    10321 accaaggccg cagccccggg cggcgcgacc ggccgcatca cccaggtcat cggcgcggtc
    10381 gtcgacgttc agttcgatgg cgccctgccc gaaattctca acgcgcttga gaccaccaac
    10441 ggcggcaatc gcctggtgct cgaggttgcc cagcatctcg gcgagaactc ggtgcgctgc
    10501 atcgcgatgg acacctccga gggcctggtg cgcgggcagg aagtgatcaa taccggcgcc
    10561 ccgatcatgg ttccggtcgg cgacgagtgc cttggccgca tcatcaacgt catcggtgaa
    10621 ccggtggacg aagccggccc ggtcaagacc aagacccgcc gcgctatcca ccaggacgct
    10681 ccttcctacg ccgaccagtc gaccgaggca cagatcctcg tgaccggcat caaggtcgtc
    10741 gacctgctcg ctccctacgc caagggcggc aagatcggcc tgttcggcgg cgccggcgtc
    10801 ggcaagaccg tgctgatcat ggaactgatc aacaacgtcg ccaaggcgca cggtggctat
    10861 tcggtgttcg ccggcgtcgg cgagcgcacc cgcgagggca acgacctcta tcacgagatg
    10921 atcgagggcg gcgtcaacaa gaagccggag aacggttcga ccgccggctc caaggccgcg
    10981 ctggtttatg gccagatgaa cgagccgccg ggcgcccgcg cccgcgtcgc gctgaccggc
    11041 ctgaccgtcg ccgagcattt ccgcgaccag ggacaggacg tgctgttctt cgtcgacaac
    11101 atcttccgct tcacccaggc cggctcggaa gtctcggcgc tgctcggccg catcccgtcg
    11161 gcggtgggct atcagccgac gctcgccacc gacatgggcg cgatgcagga gcgtatcacc
    11221 accacgacca agggttcgat cacctcggtg caggcgattt acgtgccggc ggacgatctg
    11281 accgacccgg cgccggccac ctccttcgcc catcttgacg ccaccaccgt tctgtcgcgt
    11341 tcgatcgccg aaaagggcat ctatccggcg gtggatccgc tcgactcgac ctcgcgcatg
    11401 ctgtcgccgc tggttgtcgg cgcggagcat tacgaggttg cccgcaaggt gcagtcgacg
    11461 ctgcagcgtt acaagtcgct gcaggacatc atcgcgatcc tgggcatgga tgagctttcc
    11521 gaagaggaca agctggtggt cgcccgcgcc cgcaagatcg agcgcttcct gtcgcagccg
    11581 ttccacgtcg ccgaagtctt caccggctcg cccggcaagc tggtcgcgct ggaggacacg
    11641 atcaagggct tcaaggggct ggtcgagggc aagtacgatc acctgccgga ggcggccttc
    11701 tacatggtcg gcaccatcga ggaagcggtc gaaaaggccc agaagctcgc ggctgcggcc
    11761 gcctgatatc agggcaggag agccgcgatg gccagtttca acttcgaact cgtctcgccg
    11821 gagcgcatcc tgttctccgg ccctgtcgat caggtggtcg tgccgggcga ggatggcgac
    11881 atgaccattc ttgccaacca cgccccggtg atgaccaccc tgcgctcagg catcgttgcc
    11941 gtcagcgccg acaagggcgc cgccaggcgc ctttatgttc gcggtggatt tgccgatgtc
    12001 accgcgtccg gtctgaccat cctggcggaa gtggcgatcc cggtcgagga aatcagcctc
    12061 gacaagctcg ccggcgatat gaagaacgcc gaggatgacc tccgcgacgc caagacggac
    12121 gaaacccgca aggctgccga aatgcggctt gccggtcttc gcgccatgca ggcggccctg
    12181 cggaactgaa cctgacacgg gacggacaaa aaaaggggcc gaatggcccc tttttctttg
    12241 tgccgcccgc ttgccggcgc ttgaaatcag aacgaggcgc ccaccgtgat ctgcccgcga
    12301 tgggtaacct ggctcttctg tccgacggtc gcgcgatagg tcgcgcttgc cgcccagcca
    12361 ccgatctgcg catcgagccc aagccccagc agcaggccag ccccgaccgg atccttgaga
    12421 ttggccgaga acgacatcgc ggtattggcg atcagccgtc cgcccagctt cccggcggac
    12481 gaatagagaa actcctcata gccgatgagc gcatgcgcgc gtgtgccggc ggcgacagtg
    12541 gcatcgacgc gcagctcggc gcctgccgcc agaccctgca cagtgcggcc ggcgaacgca
    12601 atcggtgcga cgactccgct ctcgttgaac ttgttgaccg aggcgctcac ataggaaagc
    12661 cgtgccttcg gcgtcaccgc ataactgccc atattccagc gatagcctgc ttcggccacg
    12721 gcgctatagc caacgccggt cgtctggccg gaattggtca gattgccaac caatgtcttc
    12781 cgctcgatct cgcgataatt ggtcacggtc gcgccgagac ccagattgac aaaggtcggc
    12841 ccctgcgacc agcgggcagc cagatcagcc gcaaaggcaa ccggcgagaa actgaccgtg
    12901 cctgacttgg cgtctccgac agtggccgaa aggccaatgc ccgccgtcca ggccggattg
    12961 aacttgtgga acacgccgaa gcgcaggccg cccgccgcat agcgcgcgcc ggcatcggtg
    13021 ctcagcagcg cattcccggt aaagcctgcg gtcacaaggc tggaattgcg cgagccctgc
    13081 tcgccgatcg cgctgatgaa atactcgggc ttgccttcgc caggtgccgc gacctgcacc
    13141 cggtccatca tatcgcccat cgcctggcgc cgcgaccaga agccgacatc gccaagtccg
    13201 cccatcgcca tcgcccttgt cggcgcgaac agatattcct gcgcgaagcg ggcgatcatc
    13261 tggtggcccg tcgcggtcgg atggacgccg tcccagaaca ggaaggtggc ctgtgtcgcc
    13321 gcgttaccgg tcacgcaggc aggggtcaga atgcattggt tggtaacatt cgaaaggcca
    13381 aacgcccccg gattggcaat tgccgccgtc aacagcgcat cgatcggcac ctgaacgaca
    13441 ttggtcgcgg gattggccgc agcggctgcc gataccgcag tgttccacgc gccattgaac
    13501 gtcgcggtgg cgtaggagcc gagctgcacg ccgagcgggc cctgcgccag atagttcggc
    13561 gctgcgccga gattgggcag gttcatcaca aggatgatcg gcgcgccggc cccggccagc
    13621 gtgcggacct gattgccgac attggtcgca gcactggtcg cagcgaccgt gaccgctgcc
    13681 tgagtgggcg ccgactggaa gatgtcgttg gcgccggccc acagcgtgac cagatccgtt
    13741 gtggtgaacc gcccgccaag cccgatgtaa ttgttgatct gcgtgaccgt tcccggcggc
    13801 atcgccacgg cgctgtcggt gcgcgcgccg ccaaaggcga agttgagatt ggcggtcggg
    13861 gaaagggcag ccggcagcgg cggaatgccg aggatcgagc cgccagcgcg ggtcatcgga
    13921 ccgttcagca gttcagccca aaccggacca ttggaaaacc gtccctgaaa atagggagcc
    13981 ggcggtgttc cggcgagcga gaacaggttg ccgttatccg acaggctgtc gccgaaggtg
    14041 atgatacggc tgaacgactg ggcatcggcc agcgacggca tcaacgcggc accggcaagc
    14101 agcatggctc taagacgcat ggattcctcc cccggccgca aaggcccatt ttcaatcaga
    14161 tcttgatcac gacactagcg cgcgccttgc caatggcaac tgttcgtgac cggcaggcct
    14221 gcctcatggg tcatttgtga accgatgtgt tggatttgca tcagcttggc acgccttcgc
    14281 ttgactttgc cgcgccggct ccctttctgg gcgcaagctc cgccagggag agaaggatgc
    14341 tgctgcccga tatccgcgac tatgaccggc tctatcgttc gtttcgctgg gacattccgg
    14401 cccgtttcaa catcgccacg tctgtctgcg atgtctgggc tgaacgggac ggggatcggc
    14461 ccgcgatcat cgaagtccct gtcagcggcg acgtcaggat caccagctat cggcagatgc
    14521 gcgagcagtc gaaccgcctg gccaatgcgc tggtgcggca cggcatcgcg gcaggcgacc
    14581 gcgtcgccat cctgctgccg caaagcgcgg ccgttccggt ggcccacctc gccgtctatc
    14641 gcatgggggc aatcgcattg ccgctggcgg tgttgttcgg ggtcgatgcc ctgcaatacc
    14701 gcctcaccaa cgccggagcg cgggtgctga tcactgatgc ggccggcgtc ggcaagatcg
    14761 cagcaatccg cggcgctctt ccgggcctcg aactgattct gtccgtcgat ggagacgacg
    14821 gcgcggccat cggcctcgat cgtttcgttg ccggtgccgg cgccgatttt cccaccgcgc
    14881 ccaccggtcc ggacgaccct gccctgatga tctacacctc cggcacgaca ggccagccaa
    14941 agggcgccct gcacgcccat cgtgtcctgc tcggccatat gccgggcgtg cagtggccgc
    15001 atgaattcat gccgcaaccg ggcgacaggg tctggacgcc ggcggactgg gcctggatcg
    15061 gtggcctgat gaacgtcctg atgacctcgc tcgcctgcgg cgtgcctgct gtggcgcatg
    15121 tgttcgagaa attcgacccc gaggcagcct tcgccttcat ggcacagcat caggtgcgca
    15181 acacctttat cgccccgaca gccttgcgca tgctgcgcac agtagctcgc ccgcgcgagc
    15241 gcttcggcat cagcctgcgc accctggctt cgggcggcga gacgctcggc gaggaggtct
    15301 atgcctgggg acgtcaggaa ctcagcctta ccatcaatga gttctatggt cagaccgaat
    15361 gcaacctgat cgccgcctcc tgcgcggcgg gcggggtatc gcgctccgga tcgcttggca
    15421 aggccgtgcc gggacatgtc gtcggcatca tccgcgagga tggcaccccc tgcgccgtcg
    15481 gggaaatcgg catcattgcc gcacgccggc ccgacccggc gatgtttctg gaatattggg
    15541 gacagccgga ggcaacccgc gccaaattca tcggcgactg gatgaaaacc ggcgatcagg
    15601 gccgcttcga cgaggacgga tacatcttct ttgtcggccg cgatgatgac gtcatcacct
    15661 catcgggcta tcgcatcggc cccggcgaaa tcgaggattg cctgatctcc cacccgaaag
    15721 tcgccattgc cgcagtcgtc ggaaagcctg acccggtgcg caccgagatc gtcaaggcct
    15781 tcgtcgtgct caagcccggc atcgccggcg acgaaaccct cagacgcgac atccagtcct
    15841 atgtgcgcac gcggctctcg gcgcacgaat atccgcgcga gatcgaattc gtcgactccc
    15901 tgcccctgac caccaccggc aagatcatcc gtcgcctgtt gcgcgaacaa cggtagatca
    15961 cgctctcgtt ttgattgaat catcaaaacg agagacaacg tgatcgttat caagagttta
    16021 gagcatgctt gttgcgatta gccgggttcc acctaatcgc agcatgctcc atctagcgcc
    16081 catcgatgcc tgtgctcacg cggctctggc aaagaccctg acacgctgcc gcaacccctt
    16141 tagggaaccg atgacccgac gccgtgcagc ctcaccaaag accgccatcc tgaaccggac
    16201 gggcgaaagc ggcgcaagat cgatcatcag atcacacagc tcgcgccgcc ccgtccagac
    16261 tgcctcgatc atcctgtggc cggcatgcgc acaggaatcg atcatcgtga acgcctgagt
    16321 ctcgactgcc cagttcgaca atttgtggat catctggaca ccgggagaaa agcgggcatg
    16381 ggtctcgtcg aaggcgattt tccacaaaaa gccggtcgtc ccggcgcaga ggacgagacc
    16441 accggcgatc atcttgccat cgagttcgag acgggcgact ttcaatcggc cacgacgtgc
    16501 ggcactccac agcatcgccc gggcaaaatt ggcggtttca gcatggttga gcagcgatgt
    16561 cccggccctg cccttccagc cgctcgcctc aagcgccaga aaatcctcga tccccgcctt
    16621 tagatcggcg tagccgtcca ccacgccgaa cgataactgc ccctgttcgg ccagccgccg
    16681 ggactgccgg cgcaattcct tgccggccgt ttcaagctga gcaggaatgg actttgccgt
    16741 tgcaaatacc gcccgctcgt aacgcgccag catcgtgatg cggctgcccg ccttggcgac
    16801 tgcccgctgg atcgccacca tgatgggacc atcggccgcg atctgcgaca gtttcaatgc
    16861 cgcatccttg ccagacagca ttctgtcgat gaaagcctgc acaaccgcat cgcattcgct
    16921 gcgatcgacc agcggctgcg acaaaaccac gaagggatgg gtccagcccc cgatggtctc
    16981 gcccatccgc agcttgtgaa cctccgccaa tggccagaga ccgaccagcc gctggccgcc
    17041 gtggccggat tgggggcgcc atgccgccag acataacggt cgcgcggttc tgcggaaatg
    17101 gcggctggct gccagcagga aatcagcgcc atagaacggg ttggcttcgg aagcacgcgc
    17161 ctcaagccag cgcagatcgg gctcgatccg ggcaagatca cctgctgcca gttcttcaac
    17221 cacgattgcc gcgtctgagg ggcgagtggc atgcagaagg ctcgccggaa cctcgccctg
    17281 atacagcgcg gggcccacca gattgggcag tcttgtgtca gtaaagacct gcatcctgat
    17341 ccgtttctgc acgactgatc cgcaacgaga cggcatgtcg ggcaatcgat tgcgactgcc
    17401 gatccgttcc aaactccgtg ccagatggtc aggaccggcg gtcatagggg cgcaacgcct
    17461 ggcgcagcag cgcgcggcga tgatgctggc cacgcgagcc ggctgccggc cctgccccgg
    17521 accagaacgc agtcagcgcc ccgcttagag cattggggag gagacggggc acctcctgat
    17581 tggtttccgc aaactgcgaa tcgacggccg gatcgacgac aagccagtcg gccacggcaa
    17641 gtctcctgcg atcaaacgcg gcctgcccct cggtcaaatc atcggacccg gcgacggcca
    17701 gaaccgcaac aggcgcaaag cgcagcgaca gatagggatg acccctgatg gcccctgcca
    17761 gagcggccgg gtctgcggcc cggtctgccg caatgaccag gcggcgaatg ggccgcatgc
    17821 ggtgattgtc gacgcttctg agcacatcct ccagcgccag caccaggtca tcccgccccg
    17881 tgcagcacag gcatgccccg cttagccgat cggcagctcc gtcaggcaac gccattgctt
    17941 cggcgcgacg cggctggccg agcgccccgg atgccagcag cagcgtcgcc ccgtcgtcgg
    18001 ctgaggccag gaccgcttcg agcacatcgc cccgggctga caattcggcg ccgctgacca
    18061 ccagcaccgg gacgggctgc ggacgcatca ccgtactcat ggctcgaatg gcgtgatcgg
    18121 ttcgctgcgt ctgagatgcg gcagcaggtc gaaggccagt gcccctacca ccagcgcgat
    18181 tccaaccgtc tgggccagcg tcaatgtttc agacaggacc agtgctgcgg ccaggatcgc
    18241 caccacaggt tcgaacagga acagcagccc cgccgttgcc gggctgatcc gcgccgccgc
    18301 catgatctgc agaaggaagc cgatgaaata ccccgcgaca ttgaccagca gcggcgccag
    18361 gccggccatc agaactgccg gctcggagag gccacccatc acatgggcaa agcccaagcc
    18421 aagcggcagg gcacagatct ggcaccagaa gaacgtcacc gaactgtcgg tctcgacccg
    18481 cgccgcagaa atgaacatcc cggcgcaggc ggcgcttcca aacagggcaa gcaccacacc
    18541 gcgcggatca agcctgtgtg cctcggggcc gaccgccagc agcactccgg caaatgccac
    18601 cagtgccacg acccagcgcc gcagcgacaa tggcacccgg tccacatagg gcgacaacag
    18661 gatcacgatc agcggatagg tgtagaagat cgttaccgcg atcggcaccg gcagataggc
    18721 aagcgaactc atgtagaaca gcgccgttgc aaccgaaaaa accgcaaagc ggaaaaccgg
    18781 tgcccgattg cccgggctca gtgccagccg ttggccgcgc cacagtgcca gtcctgccag
    18841 cagcggcagg aacagaaagg cgcggtagac cacgatgtca gaaccgctga tgccaagctg
    18901 ggcacacagg cgtgccccga caatgttgat gccataggca gtcgcgccaa ccagcgcgag
    18961 ggccgatcca accagcgtgg cacgccggac cgccgcgccg gcatcaccaa gtcctgtcat
    19021 tcgtcgtcat cgtcagcagc agcacgcttc ttggctggct gcacaggggc tttggctttg
    19081 gccagcgcag cagcagcggc agggcgcgcg gcctgcgacg gctggccagc ctgcactttt
    19141 gccccggccg g
//
LOCUS       DAOMXG010000029        18950 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_1694_length_18950_cov_11.583911, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000029 DAOMXG010000000
VERSION     DAOMXG010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 18950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18950
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1694_length_18950_cov_11.583911"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PLQ11_03475"
     CDS             complement(<1..255)
                     /locus_tag="PLQ11_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK23995.1"
                     /translation="MALRGKIAFVALLAAGAALAQQTQKSPAAPVVQTVAGGYDLTSA
                     DGSRKCLMLLRPADSPGGHALGFPAPCRLAFPLLVSVAAWS"
     gene            complement(266..1237)
                     /locus_tag="PLQ11_03480"
     CDS             complement(266..1237)
                     /locus_tag="PLQ11_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HRK23996.1"
                     /translation="MSAFQVAIHGPVMPLIRDGLSRRLPTHLLWEKPDLATFLKDQGA
                     GIRALVGGFRTRIDASELDRFPNLKIISNFGVGYDMVDAAAAAERGIVVTNTPDVLNE
                     EVADTAIGLLLMTVRKLPQAERYLRAGRWEKEGNYPLTASLRGRSMGIVGLGRIGKAI
                     ARRAEAFGLKLAYYGRTRQADVAYPFYSDLVAMARDVDILMLILPGGAATYRLVDAAV
                     LDALGPDGILINVARGTVVDEPALLAALQGNRILGAGLDVFEHEPKVPAAFFDLDNVV
                     LLPHVGSASRHTRDLMGRLVVDNVLAIAEGRPPLTPVAETPWPLSKG"
     gene            complement(1338..1802)
                     /locus_tag="PLQ11_03485"
     CDS             complement(1338..1802)
                     /locus_tag="PLQ11_03485"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HRK23997.1"
                     /translation="MSTGTGEWLQRAARRHRQRLTVAFEGLGFFPGQEGLVLHLAENG
                     AMTIGALAEVLKVRPPTVSKTVNRLLAQGLIERNSIAEDARKSSVALTPEGIKRAKVL
                     AGRLDKVEADIADALDDKTHRRLRKGLKKLTNALSPADQDSAEADDTDGDED"
     gene            1873..4086
                     /locus_tag="PLQ11_03490"
     CDS             1873..4086
                     /locus_tag="PLQ11_03490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HRK23998.1"
                     /translation="MASVHDNLVTLDVSGMTCAACAARLQRVLAAVPGVVSADVSLPL
                     ERADVDWPAGQSVQVLIGAVEAAGFHANLRGASSEARRIAREARAIRRRQEDRRTLLL
                     AALACGLALPFWIDMLRMMASGDHAPLLHPAVQAVLAGLAQLICGARFYRGAAAALRG
                     GAANMDVLVVLGTSAAYALSLFNLLADRVHHGGGLYFEAGVTVLAFLLIGKVMEGRAR
                     HGASEALEALAAGAPRHALRIVDGVETLVEAASLKPGDVVAVRPGAQVPADGRIIEGA
                     AAFDESLLTGESLPVARGVGDAVIAGAIASGGRVLVDVTASGDDTRLQRIGRLIEDAD
                     IARSPTQQLADRISAVFVPLVIAVALVAGIGWWLALGALEPALLTAVAVLVVACPCAL
                     GLATPIALVAGANAAARAGLIVSDHAALDTAGRLDRVAFDKTGTLTCGVADVVGIAVV
                     RGTALEALRLAAALAARSDHPLDQALVRRASVQGLALPPADEFSAEPGRGVRGRVEGR
                     MICMGNAAFLAEQGVAIVEPASVLDDPSLAAAGSLVLLADEAGLIAAIGFADAPRPET
                     AEALHRIAALGLKLTVLSGDRPEAVAAFARPLGLGDARAGLAPDAKIRMIEAMRRDGE
                     VVALAGDGVNDAPALRAADLGIAMGSGTEAAKAAASVTLARPDPRLVATLIEAGRATR
                     RTIAENLAFAFLFNGVGIPLAAMGKLSPVLAGAAMAASSVSVALNAWRLAIRRFG"
     gene            complement(4096..5688)
                     /locus_tag="PLQ11_03495"
     CDS             complement(4096..5688)
                     /locus_tag="PLQ11_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0009250 - glucan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein G"
                     /protein_id="HRK23999.1"
                     /translation="MTINRRTLMLASGTILTPALLGTVGSAPAQTGPAAPVGNGQPPK
                     PRFRFDDVVKKASEALNVPFDINPAPLPEPIARLDFDTWRDIRFKPDKAFFNQPGSPF
                     RLQLFHPGFLYNRPMTVNLIRDGIPTPVPYAANLFDYGRVKVERPLPVGLGFAGFRLH
                     APLNDPRVHDELISFLGASYFRFLSRDQRYGLSARGLAINTGRKEGEEFPVFTDVWIE
                     TPDADADRVTLYALMQSASLTGAFQFVVFPGPETVVEVVATVFARQPVARLGLAPLTS
                     MFLAGENDQRHSDDYRPELHDSDGLLIHSGSGEWLWRPLRNPKEVETSAFLDTNVRGF
                     GLMQRDRTFEHYQDLDLAYERRPGYWIEPHENWGEGQIELIEIPTSDETNDNIVAFWR
                     PKAPLEPGQNLTIRYRMRSLSGTADLNPGGMALNTFRTRARALGSGETPPAGSTRFII
                     DFAGGDLAFQMLAPQSVQIVASASVGKVTRAALMPNPSIQGFRAMIDIEAPPGQLTDL
                     RAFLKAGSRVLTETWTYPWRAE"
     gene            6365..7495
                     /locus_tag="PLQ11_03500"
     CDS             6365..7495
                     /locus_tag="PLQ11_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HRK24000.1"
                     /translation="MTERIREFLRTRRDDGPCVVVDLDIVRDNYLTFAKALPDTRVFY
                     AVKANPAWEILELLTKLGSSFDCASLGEIEDVLAAGATADRISFGNTIKKERDIERAY
                     ERGVRLFAVDCEAEVDKIARVAPGSKVFCRILCDGKDAEWPLSRKFGCEPEMAPRVLE
                     HAHRSGLSAYGVSFHVGSQQRNPEVWDGALASAAWIFREMAERGITLSMVNLGGGFPT
                     KYLKEIPGVPTYGQAIFRSLRKHFGNRLPETIIEPGRGMVGNAGIIETEVVLVSKKSD
                     KDTVRWLYLDIGKFHGLAETMDEAIRYPIKTPRDGDDLVPYIVAGPTCDSVDVMYEKE
                     PYLLPFSIEIGDKLLIEGAGAYTTTYSSQNFNGIPGLRQYVI"
     gene            7602..8162
                     /locus_tag="PLQ11_03505"
     CDS             7602..8162
                     /locus_tag="PLQ11_03505"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HRK24001.1"
                     /translation="MLRILEEHPSDAADREALLDAAVGADRFLKTSERLREGRLPAAG
                     LALVARDGEALVGTLRLWHVATGDGRPALMLGPLAVAGSHRDQGIGGKLMRRGLTIAG
                     ARGHGAVLLVGDEPYYRRFGFSRTLAKALDLPGWYQPERFLGLELKPGALTGACGMVH
                     ATGPLDPAIDTPATLSMKAASGAEMR"
     gene            8252..9685
                     /locus_tag="PLQ11_03510"
     CDS             8252..9685
                     /locus_tag="PLQ11_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homospermidine synthase"
                     /protein_id="HRK24002.1"
                     /translation="MTQWPNHGTISGPIVMIGFGSIGKGTLPLIERHFSFDRKRLVVI
                     DPEDKDKALVESLGYRFIHEAVTRENYKDLLKPLLTVGGGQGFCVNLSVDTSSVDIMT
                     FCQEIGAFYVDTVNEPWAGFYFDASKGPEARSNYALREVTLAAKQKAQGKTTAVSCCG
                     ANPGMVSWFVKQGLLNIASDLKLSFSEPKSREDWAALMQAVGVKGIHIAERDTQRSKK
                     PKPMDVFVNTWSVEGFVSEGMQPAELGWGTHEKWMPENGRTHDKGCQAAIFLLQPGAN
                     TRVRSWCPTPGAQYGFLVTHNESISIADYFTVRDASGKAVYRPTCHYAYHPANDAVLS
                     LHEMFGAGGRRQSSWHILDENEIVDGIDELGVLLYGHGKNAYWYGSQLSIEETRRVAP
                     YQNATGLQVTSAVLAGMVWALENPKAGIVEADEMDFRRCLEVQMPYLGPVVGAYTDWT
                     PLAGREALFKEDLDRSDPWQFRNILVR"
     gene            complement(9696..10214)
                     /locus_tag="PLQ11_03515"
     CDS             complement(9696..10214)
                     /locus_tag="PLQ11_03515"
                     /inference="COORDINATES: protein motif:HMM:NF022605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2937 family protein"
                     /protein_id="HRK24003.1"
                     /translation="MIFGRLVFAGWMLASGLAGAQLPELAQQYRQRLGGAVDELRTIV
                     TRFETDAKSEGLDSAAALERHRANQDELIRKRGIAMAETIARFTRLSDQEVRLKEDNT
                     LLRVTTMLAHGDRDIVSAAMRDFSPAVPINAEGGFFAAVGAFFGWLALRLFTWPKRSF
                     DRWLAYRRYYRR"
     gene            complement(10345..11346)
                     /locus_tag="PLQ11_03520"
     CDS             complement(10345..11346)
                     /locus_tag="PLQ11_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019905530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HRK24004.1"
                     /translation="MAGQNDTLTRHVVAIATGEHVTAAVFLGEAPALALGDGSIRLGW
                     GQGARAVPAHPDAAILVAAADGRRMVTGGDDGRVVLTDAEGGLTVLAEEPGRWIDAVA
                     LGPNGSAAWSFSRTVRARDAKGTVRSLAAPSSSRGLAFAPKGYRLALSHVDGASLWFP
                     NTEAAPEKLDWKGVHLDITWSPDGRFVVSSMQENQLHGWRLPEKTHMRMSGYPAKTRS
                     LSWSSDGEWLATSGAEAAIIWPFSGTGPMGKAPRECGVRPHRVTRCAFHPKALVLAIG
                     YEDGLVMLVRLTDASELLVRNAEAGGAITALAWDGAGNRLLFGTEDGAAGLLTLPGS"
     gene            complement(11353..12342)
                     /locus_tag="PLQ11_03525"
     CDS             complement(11353..12342)
                     /locus_tag="PLQ11_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24005.1"
                     /translation="MVRTGTKGIGIAAMLALATSAMAADRNPAPSAEAAPSGWENGGD
                     IYGFSDNPDTPGKVGPANVKLGWIGLNGKRFGRYQVLTTTLESEYGLADWLSVTPSIS
                     LGAHNLNDVPLVLGDRLAALAAGETARDAYFYPNRNRGSFSALAAGFKMRLIERGKLV
                     NGQLEGSPIGLAVTMEPKWTAVDDATGNRLTAFSFASAIIADTAIIPERLFWTVNLGA
                     GLTSGRDRDARAPVHASSLALSSALSYQFAPGVFLGVEARHERAYDGNSWKTYLGNAT
                     YLGPTLFLKSGNWGLQAAWNAQIAGHAKDEPGQKLNLSQFERHRARLRLSYDF"
     gene            complement(12356..13318)
                     /locus_tag="PLQ11_03530"
     CDS             complement(12356..13318)
                     /locus_tag="PLQ11_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018259748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRK24006.1"
                     /translation="MSDKIPVTVLTGYLGAGKTTLLNRILTENHGRRYAVIVNEFGEV
                     GIDNELVVGADEEVFEMNNGCICCTVRGDLIRIVEGLMKRRGKFDAIIIETTGLADPA
                     PVAQTFFVDQDVQAKARLDAIVTVADAKWLSTRLKDAPEAKSQIAFADVILVNKTDLV
                     SEAELLEVEARIRAINPYARLIRTLKCQIPLSEVLDRGAFDLDRILDLEPDFLDPGHH
                     HHHDEAMQSISVTIDGEVDPERFMPWINDIVQRDGPDILRSKGILAFPNEPKRFVYQG
                     VHMILDGDLQRDWRSDEKRTSKIVFIGRNLKVDEIRKGFLACKA"
     gene            complement(13335..14264)
                     /locus_tag="PLQ11_03535"
     CDS             complement(13335..14264)
                     /locus_tag="PLQ11_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HRK24007.1"
                     /translation="MPVTMLDRRSILASAATLLGTLPLAAQQSPVTVVASFSILGDLV
                     RAIGGDRLAVKIIVGAGQDAHVYKPTPADAKTIAGAALIVVNGLGFDTFMERLVRSSA
                     SKSPVVDLSKGVSVLKEAKQAHGHDHGHKHGGNQDPHIWQSIGNVKQMVTTLADALAK
                     VDPAGAATYAANLAAYRSKLDALETELKALVATVPDDRRLFATTHDAFAYLARDFGLK
                     PLALQGISTNAEPSAADIARIIRQLKALKAPAVFLENITDPRKIERIAQDSGARVGGT
                     LYSDSLSLADGPVPTYIDLMRHNIMTLVKALKP"
     gene            complement(14269..15141)
                     /locus_tag="PLQ11_03540"
     CDS             complement(14269..15141)
                     /locus_tag="PLQ11_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HRK24008.1"
                     /translation="MGFYDLLIGPFVEFGFMRRALAGCLVLASGAAPIGVFLMLRRMS
                     LIGDAMAHAILPGAAAGYLLAGFSLGAMTVGGLVAGFAVAVLAGIVARTTTLKEDAAL
                     AGFYILSLAAGVTLLSARGSNVDVLRVLFGSVLALDDPTLWLMLAVTTITLISLSALF
                     RGLVLDSVDPGFFASVSRSAAALPLVFLGIVVINLVSAFHALGTLLAVGILILPAASA
                     RLWSNDLTRMIALAWAFATLASLVGLIASYHFGLASGPTIILAAGMIYVLSLAFGRTG
                     GIVRTVQPSQHLKG"
     gene            complement(15144..15881)
                     /locus_tag="PLQ11_03545"
     CDS             complement(15144..15881)
                     /locus_tag="PLQ11_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRK24009.1"
                     /translation="MASAIRFDNLTLGYDRHPAVHHLTGTIPHGALLAIVGPNGAGKS
                     TLMKAMAGFIRPLGGRLERNVKDRRDLAYLPQVVEIDRSFPISVYEFVSMGAWNAVGL
                     FGGIDRPTRLRIEHALSAVGLEGFESRAIGTLSGGQMQRTLFARLLMQDASMILLDEP
                     FNAIDQKTTEDLVVLIRRWHSEGRTVIAVLHDLPLVRAVFPETLLLARETIAWGPTLV
                     SLTQTNLDAAQRMVEAFDADAHICERA"
     gene            complement(16139..17698)
                     /locus_tag="PLQ11_03550"
     CDS             complement(16139..17698)
                     /locus_tag="PLQ11_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein D"
                     /protein_id="HRK24010.1"
                     /translation="MDRRTLFAVLAAAALPSRQISAQEPGLDLGPAATFSFDALVERA
                     RLAAASAYVPPPRPAPDVLERIDYDAHGKLRFKTDFAVWGRPQEQRRFPVTFFHLGRY
                     FVKPVRMHVVDGGKAREIIYREDYFDMPADSPAKQLPKGAGFAGFRFQEAVDGTLDWK
                     KNDWVAFLGASYFRAIGDQFQYGLSARGLMLDSAIGTPEEFPDFTHIWLEQGQSAGHV
                     TVYALLDSPSVAGAFRFDMKRGRGVTMDIDKRLFVRKAVARFGIAPLTSMYWFSETAK
                     PTAADWRPEVHDSDGLALWTGSGERIWRPLNNPPRTLASSFGDTNPRGFGLLQRDRAF
                     DHYQDGVYYERRPSLWVEPVGNWGKGSVTLIEIPTDDEIHDNVVAAWVPEAPVQPGDK
                     FALKYRLHWQAEEPYPTTLARVLATRMGNGGQPGQPRPKGVRKFMVEFLGDPLKALPS
                     GVRPEAVLSASRGSFSYVFTEAVPNDVAGHWRAQFDFHAEGTEPVEMRLFLRLDGQPL
                     SETWLYQYHPF"
     gene            complement(17736..17966)
                     /locus_tag="PLQ11_03555"
     CDS             complement(17736..17966)
                     /locus_tag="PLQ11_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003611506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24011.1"
                     /translation="MAKRLNASNLLTIVSVTILVGVEVIGALLAAGWALGGLFQLGRE
                     VTWGIMAVCIAAGGWATFRFLQGALKIEPIYQ"
     gene            complement(18056..>18950)
                     /locus_tag="PLQ11_03560"
     CDS             complement(18056..>18950)
                     /locus_tag="PLQ11_03560"
                     /inference="COORDINATES: protein motif:HMM:NF015763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nickel transporter"
                     /protein_id="HRK24012.1"
                     /translation="LFGGLVAWILAKQVEFNRAMITALRATREGAGAWALISVAFLYG
                     VFHAAGPGHGKAVVSSYVFANEDVLKRAVLVALAAALLQAVVAITLVVPAVSLLGATA
                     RQVDQSVAWIEIISFSAILLLGLSLLWRKGRAFLAFFKQAPDQSLAPCPHCGSGLGIG
                     SHTHQHHGHHHDHGAACTHVHLPDATDLAGTKSWRDLVAVVVAAGSRPCSGAIILLAF
                     ALSVGALGVGIAAVFAMAVGTGLTTAGFAVGAVLAKGATRRLAERSERLAWLTAAVEC
                     AAALAVTLLGAALLMGYLTSS"
BASE COUNT         3499 a         5869 c         6259 g         3323 t
ORIGIN      
        1 gctccaggcg gccacggaga ccagcagcgg aaacgccagc cggcagggcg cgggaaagcc
       61 caaagcatga ccacccggcg aatcagccgg gcgcaacagc atcaggcatt tgcgcgagcc
      121 atcggcgctg gtcaggtcat agccgccggc gacggtctgc accacggggg cggcgggact
      181 tttttgcgtc tgctgggcga gtgcggctcc tgctgcaagc agtgcgacga aagcgatctt
      241 gccacgcagc gccatggcga ttggctcacc ccttgctcag cggccatggc gtctcggcca
      301 ccggcgtcag cggcggtctg ccttcggcga ttgccagcac gttgtcgacg acaagccgcc
      361 ccatcagatc gcgggtatga cgggaagcgg agccaacatg gggcagcagc accacattgt
      421 cgaggtcgaa gaacgccgca ggcaccttcg gttcatgttc gaagacatcg aggccggcgc
      481 cgagaatgcg gttgccctgc aacgccgcga gcagcgccgg ctcatcaacc actgtcccgc
      541 gcgcaacgtt gatcaggatc ccgtcaggcc ccagcgcatc caacaccgcc gcatcgacca
      601 gccgataagt cgctgcgccg cccggcagga tcagcatcag gatatcgacg tcgcgcgcca
      661 tggcgacgag gtctgaatag aagggatagg cgacatccgc ctgccgcgtg cggccatagt
      721 aggcgagctt caggccaaag gcctcggcgc gccgggcgat ggccttgccg atgcggccca
      781 ggccgacgat gcccatgcta cggccgcgca aagatgccgt cagcgggtaa ttgccctcct
      841 tttcccagcg cccggcccgc agatagcgct cggcctgcgg cagcttgcgc acggtcatca
      901 gcaggaggcc gatggccgta tcagccactt cctcgttgag cacatcgggc gtgttggtga
      961 cgacaatgcc gcgctcggca gcggcggcgg catcgaccat gtcgtagccg acgccaaaat
     1021 tggaaatgat cttcaggttg ggaaaacgat cgagttcgga tgcatcgatg cgcgttctga
     1081 acccgccgac cagagcccgg atcccggctc cctgatcctt gagaaaagtg gcaaggtcag
     1141 gcttttccca tagcaaatgg gtcggcagcc gacgggatag accatcccga atgaggggca
     1201 tgaccgggcc atggatggct acctgaaaag cactcacggc aaaaatcctc gcacgatggg
     1261 gttggccgag gaccaccgta aaggatgtat cgcttgtctg acaaccagga ttgcagcgct
     1321 gtgctacgct ccggctctca gtcctcgtcc ccgtctgtat cgtcggcctc ggcggaatcc
     1381 tgatcagcag gagagagcgc gttggtcagc ttcttcaggc ccttgcgcag acggcggtga
     1441 gtcttgtcgt cgagggcatc ggcgatatcg gcctcgacct tgtccaggcg cccggcgaga
     1501 accttggcgc gcttgatccc ttccggagtc agggcaacgg acgatttgcg ggcatcctct
     1561 gcgatgctgt tgcgctcgat gaggccctgc gccagcagcc ggttgacggt tttcgacaca
     1621 gtgggcggac gcaccttcaa gacctcggcc agcgcgccga tggtcattgc gccattttcg
     1681 gcaaggtgca ggaccagccc ttcctggccc ggaaagaagc ccagtccctc gaatgcgacg
     1741 gtcaggcgct gccgatgccg tcgcgccgcc cgttgcagcc attcccctgt acctgtactc
     1801 atgttgctcc ccgtggcatt tcggccggaa cgacggcttg caagccggcg gttccagcgt
     1861 catctaggca ccatggcatc cgttcacgac aatcttgtga cactcgacgt cagcggcatg
     1921 acctgcgcag cctgcgccgc tcgtctgcag cgtgtgctcg ccgctgtgcc tggagtggtc
     1981 tctgccgatg tcagcctccc gctcgaacgg gccgatgtcg actggccggc aggacaatcg
     2041 gtccaggtgc tgatcggggc ggtggaggcg gctgggttcc acgccaatct gcgcggcgcc
     2101 agttccgagg cgcgcaggat cgcccgcgag gcgcgcgcaa tcagacggcg gcaggaggat
     2161 cgccgcaccc tgctgctggc cgcactagcc tgcggcctcg cgttgccctt ctggatcgac
     2221 atgctgcgga tgatggcaag cggcgatcat gctccgctgc tgcacccagc tgtgcaggcg
     2281 gtgctggccg gtctggccca gcttatctgt ggcgcacggt tctatcgcgg cgctgctgcg
     2341 gccttgaggg gcggggcagc caatatggat gtgctggtcg tgcttggcac cagcgcggct
     2401 tatgcactga gcctgttcaa tctgctcgcc gaccgggtcc accatggcgg ggggctctat
     2461 ttcgaggcgg gggtgacggt gcttgccttc ctgctcatcg gcaaggtgat ggaggggcgt
     2521 gcccgccatg gtgccagcga ggcacttgag gcattggcag ccggggctcc gcgacatgcg
     2581 ctgaggatcg ttgacggggt ggaaacactt gtggaggcag cgagccttaa gccgggggat
     2641 gtggtggcgg tgcgccctgg ggcgcaggtg ccggccgacg ggcgcattat cgaaggcgct
     2701 gcggctttcg atgaatcgct gctgaccggc gagagcctgc cggtggcgcg cggcgtcggc
     2761 gatgcggtga tcgccggagc gatcgcttcg ggcgggaggg tgcttgtcga tgtcacggcg
     2821 agcggcgatg acacgcggct gcaacggatc ggccgactga tcgaggatgc cgatattgcc
     2881 cgttcgccga cccagcagct ggctgaccgg atttcagctg ttttcgtgcc gctggtgatt
     2941 gcggtagcgc ttgtggccgg catcggttgg tggctggcac tcggcgcgct tgaaccggca
     3001 ttgctgacgg cggttgcggt gctggtggtc gcctgtccct gcgcgctcgg tctggcgacg
     3061 ccgattgcgc tggtcgctgg cgccaatgcg gcggcacggg cggggctgat cgtgtccgac
     3121 catgcggcgc tcgatacggc cggccggctt gaccgggtcg cgtttgacaa gaccggaaca
     3181 ctgacatgcg gtgtcgcgga tgtggtgggc attgccgttg tgcgcggcac agcgctggaa
     3241 gcactgcggc tggctgcagc ccttgccgcc cggtccgacc atccgctcga tcaggcactg
     3301 gttcgtcggg catccgtgca ggggctggca ttgccgccag ccgacgaatt ttcagccgaa
     3361 ccgggacgcg gcgtgcgcgg cagggtcgag ggccgcatga tctgcatggg caatgcggca
     3421 ttcctggcgg aacagggtgt ggcgatcgtc gagcctgcgt cggtgctgga cgatccgtcg
     3481 ctggccgccg ccgggtcgct ggtgctgctt gcggatgagg ctggcttgat cgcagcaatc
     3541 ggatttgccg atgcgccgcg cccggaaacc gccgaggcgt tgcaccggat tgctgctctt
     3601 ggcctcaagc tcaccgtgct gtccggtgac cggccggagg cggtcgcagc cttcgcccgg
     3661 ccgttgggac tgggcgatgc ccgtgccggc cttgcgcccg atgccaagat ccgcatgatc
     3721 gaggcaatgc gacgggacgg ggaggtggtg gcgctggccg gcgatggcgt caatgatgcg
     3781 ccggcgctgc gtgcggccga tctcggcatt gccatgggca gcgggaccga ggcagccaag
     3841 gccgcggcct ccgtcaccct ggcccggccc gacccgcgtc tggtcgcaac cttgatcgag
     3901 gcgggacggg cgacgcggcg caccatcgcc gagaacctgg ccttcgcctt cctgttcaac
     3961 ggcgtcggta ttccgctggc ggcgatgggc aagttgtcgc cggtgttggc aggagcggcg
     4021 atggctgcgt cttcggtctc ggtggcgctg aatgcctggc ggctggccat ccggcgcttt
     4081 ggctgaggct gctggctatt cggcccgcca cggataggtc caggtttcag tcagcacacg
     4141 cgagccagcc ttgagaaagg ccctgagatc ggtcaactga ccgggcgggg cctcgatatc
     4201 gatcatggcg cggaagccct gtatcgacgg gttcggcatc agggctgcgc gcgtgacctt
     4261 gccgaccgac gccgaagcga caatctgaac cgattgcggg gccagcatct ggaaggccag
     4321 atcgccgccg gcaaaatcga tgatgaaccg cgttgacccg gccggtggcg tctcgccgga
     4381 gccaagggcc cgggcccgcg tgcggaaggt gttgagagcc atgccgccgg ggttgagatc
     4441 ggcggtgccc gagagcgagc gcatgcgata gcggatggtc aggttctggc cgggttcgag
     4501 cggcgccttc ggacgccaga aggcgacaat gttgtcgttg gtctcgtcgg aggtcgggat
     4561 ctcgatgagt tcgatctgcc cttcgcccca gttctcatgc ggttcgatcc agtagccggg
     4621 ccggcgttca taggccagat cgagatcctg ataatgctcg aacgtgcggt cgcgctgcat
     4681 cagaccgaag ccgcggacat tggtatcgag aaacgccgag gtctccacct ccttcggatt
     4741 gcgcagcgga cgccacagcc attcgccgga gcccgaatgg atcagcagcc cgtccgaatc
     4801 atgcagttcg gggcgataat catcgctgtg gcgctggtcg ttctctccgg caaggaacat
     4861 cgaggtcagc ggggcaaggc caagccgggc aaccggctgg cgtgcaaaca ctgtggccac
     4921 aacctcgacc acggtctccg gcccaggaaa gacgacgaac tggaaggccc cggtcagact
     4981 tgcgctctgc atcagggcat agagcgtgac acggtccgca tctgcatccg gtgtctcgat
     5041 ccagacgtcg gtaaagacgg ggaattcctc gccctccttg cgaccggtgt tgatcgcaag
     5101 tccgcgcgcc gacaggccat agcgctggtc gcgcgacagg aagcggaaat agctggcgcc
     5161 caggaaggaa atcagttcgt cgtgcacacg cggatcattg agcggggcgt gcaaccggaa
     5221 gccggcaaag cccaacccga ccggcagcgg gcgctcgacc ttgacgcgcc cataatcaaa
     5281 gagattggca gcatagggaa ccggcgtggg aatgccatcc cggatcaggt tgacggtcat
     5341 cggccggttg tagaggaagc cggggtgaaa caactgaagc cggaaaggac tgccgggctg
     5401 attgaaaaaa gccttgtcgg gcttgaaacg gatgtcccgc caggtgtcga aatcgagccg
     5461 ggcgatcggt tcgggcagcg gggccgggtt gatgtcgaac ggcacgttca gcgcttcgct
     5521 tgccttcttc accacatcgt cgaaacggaa acgcggcttt ggcggctggc cattgccaac
     5581 cggggcggcc gggccggtct gagccggtgc ggagccgacg gtgccaagca gggcgggtgt
     5641 aagaatggtc cccgaagcga gcatcagggt gcggcgattg atggtcacga ctgtcgtcct
     5701 gcacgttgcg gtgagcctgc atgtggcgag ccttgcgggc gccgtcaagg cagagcgttc
     5761 ggcaggcctt ctccggcatt aaccatggag caagacccat ccacaccgat ggggttaaat
     5821 tttggtcagg aacgcgattt tttcttgtaa cggcaagcca tcgaacgtat acgcggtgtt
     5881 gcccaatttc gcacgtgcct gtggctgcgt gctggtcggt ggatctccgg acaagaggcc
     5941 ggaaaaacgc caatcgtttg ggaggggagc gaggcttcgc aacccttcct gaatacggac
     6001 cagcagccgg aggcgaaccg gcggccccgc ccgaagcggg tgacgcgagc tcaaagcaac
     6061 gacggagcgg gcttttttgg tctcggtcgg ccctccaaag gctggctacc gaagaggctt
     6121 gtccatcttg ccgggcgtgc ggtcggggct cccccttcca aacgatggcg tgtcagtcag
     6181 tcggttaacg attcgttaac cgacggccag cacggcagac ggcgcagcac catcggctcc
     6241 gcaaggcgcc ggtccgggtc gcgatcgctc acgtattcct gcgtttccaa cgcgcggggc
     6301 gagggctgaa agctcccgat ctcttgaatc gctttccgga cctttccgga agagggaggc
     6361 tgccatgacg gagcgtatcc gtgaatttct ccgcacccgc cgtgacgatg gcccttgcgt
     6421 ggtcgttgat ctcgacattg tgcgcgacaa ttatctgacc ttcgccaagg cactgcctga
     6481 cacgcgcgtg ttctatgcgg tcaaggccaa tccggcgtgg gaaatcctcg agcttctgac
     6541 caagctcggt tcctcgttcg attgcgcctc actgggtgag atcgaggacg tgctggcggc
     6601 tggcgccacg gctgaccgca tctccttcgg caataccatc aagaaggagc gcgacatcga
     6661 gcgcgcctac gagcgcggcg tgcgcctgtt cgcggtcgat tgcgaggccg aggttgacaa
     6721 gatcgcccgc gttgcgccgg gctccaaggt gttctgccgc atcctgtgcg atggcaagga
     6781 tgccgagtgg ccgttgtcgc gcaagttcgg ctgcgagccg gaaatggcgc cccgggtgct
     6841 ggagcatgcc catcgcagcg gtttgtccgc gtatggcgtg tcgttccatg tcggatcgca
     6901 gcagcgcaat ccggaagtgt gggacggcgc gcttgcctcg gcggcctgga tcttccgtga
     6961 aatggccgag cgcggcatca cgctgtcgat ggtcaatctc ggcggcggtt ttccgaccaa
     7021 gtatctcaag gagatcccgg gggtcccgac ctatggtcag gcgatcttcc ggtcgcttcg
     7081 caagcacttc ggcaaccgtc tgcccgagac gatcatcgaa ccgggccgcg gcatggtcgg
     7141 caatgccggc attatcgaga ccgaggtcgt gctcgtctcg aagaagtcgg acaaggatac
     7201 ggtgcgctgg ctctatctcg acatcggcaa gtttcatggc ctggccgaga cgatggacga
     7261 ggcgatccgc tatccgatca agaccccgcg cgacggggat gatctggtgc cgtatatcgt
     7321 ggccggaccg acctgcgatt cggttgacgt gatgtacgag aaggagccgt atctgctgcc
     7381 attctcgatc gagatcggcg acaagctgct gatcgaaggg gccggcgcct atacgacgac
     7441 ctattcgagc cagaacttca acggcatccc cggcctcagg cagtatgtga tctgaggctg
     7501 tcccctcccc acgcggggag gggcgttcga caacctcccg aacggcgacc cggaaacggg
     7561 ttggtgcgag ggtctcttcc tgagggaagg agactttcga gatgctgcgc attcttgaag
     7621 aacatccgtc cgatgcggcg gaccgcgaag ccctgctgga tgcagccgtc ggggccgatc
     7681 gctttctgaa aacctccgag cggctgcgcg aaggccggct gccggccgcc gggctggctc
     7741 tggtggccag ggatggcgag gcgcttgtcg gcaccctgcg cctctggcat gtggcgaccg
     7801 gagatggccg cccggcgctc atgctcgggc cgctggcggt tgccgggagc catcgcgatc
     7861 aggggatcgg cggcaagctg atgcggcgtg gcctgacgat cgccggtgcg cgcggtcatg
     7921 gcgctgtgct gctggttggc gacgaaccat attatcgtcg ctttggcttc tcgcgaacat
     7981 tggcgaaagc tctcgatctg ccgggctggt atcagccgga gcggtttctt ggccttgagc
     8041 tcaagccggg cgcactgaca ggcgcatgtg gcatggtgca cgccaccggt ccgcttgatc
     8101 cggcgatcga cacgccggcg acgctgtcca tgaaggccgc ttccggcgcc gagatgcggt
     8161 agatggctgt ttttgattga cgcgagcggt cgggcctttc aaaaccctgc cgcctgcgtt
     8221 tttttcgttt tgcaccatgc ggagtaaaaa gatgacccag tggcccaacc acggaacgat
     8281 cagcggcccg atcgtgatga tcggcttcgg ctccatcggc aagggcacgc tgcccttgat
     8341 cgagcgccat ttcagtttcg atcgcaagcg gttggtggtc atcgatcctg aggacaagga
     8401 caaggcgctg gtcgagagcc tcggctatcg cttcattcac gaggcagtga cgcgcgagaa
     8461 ctacaaggat ttgctcaagc cgctgctgac ggtcggcggc gggcagggct tctgcgtcaa
     8521 tctgtcggtc gatacctcgt cggttgacat catgaccttc tgccaggaga tcggcgcctt
     8581 ctatgtcgat accgtcaacg agccgtgggc ggggttctat ttcgatgcct ccaaggggcc
     8641 ggaagcgcgt tccaactatg cgctgcgcga ggtgacgctg gcggccaagc agaaggcgca
     8701 aggcaagacg acggcggtgt cctgttgcgg cgccaatccg ggcatggtgt cgtggttcgt
     8761 caagcagggc ctgctcaaca tcgcctccga cctgaagctg tcgttcagcg agccgaaatc
     8821 ccgtgaggac tgggcggcgc tgatgcaggc ggtcggcgtc aagggcatcc atattgccga
     8881 acgcgacacg cagcgttcga agaagcccaa accgatggat gtgttcgtca atacctggtc
     8941 ggtggaaggc ttcgtctcgg agggcatgca gcctgcggaa ctgggctggg gaacgcatga
     9001 gaagtggatg ccggaaaatg gccgcaccca tgacaagggc tgccaggcgg cgatctttct
     9061 tctgcaaccc ggcgccaaca cccgggttcg ctcctggtgt ccgacgccgg gggcgcagta
     9121 cggcttcctc gtcacccata atgagtcaat ttcgatcgcc gattacttca ccgtgcgcga
     9181 tgcttccgga aaggcggttt accggccgac ctgccattac gcctatcacc cggccaatga
     9241 tgcagtcttg agcctgcatg aaatgttcgg tgcgggcggc aggcgccagt cctcgtggca
     9301 cattctcgac gagaacgaga tcgtcgacgg gatcgatgaa ctcggcgttc tgctctacgg
     9361 ccatggcaag aatgcctatt ggtatggttc ccagctctcg atcgaggaaa cgcgccgcgt
     9421 cgcgccctat cagaatgcga ccggcctgca ggtgacctcg gcggtgctgg ccggtatggt
     9481 ctgggcgctc gagaacccca aggcgggcat tgtcgaagcc gacgaaatgg atttccgccg
     9541 ctgccttgag gtgcagatgc cctatctcgg cccggtggtc ggcgcctata ccgactggac
     9601 gccgctcgct ggtcgcgagg cgctgttcaa ggaggatctc gacaggtccg acccttggca
     9661 gttccgcaac atcctggtgc ggtaggacgc gccgcctatc gccggtaata tcggcggtag
     9721 gcaagccagc ggtcgaacga ccttttcggc caggtgaaaa gacgcaaggc cagccagcca
     9781 aagaaggcac cgacggcggc gaagaaaccg ccctcggcat tgatcggaac ggccggcgag
     9841 aagtcccgca tcgccgccga tacgatgtcg cggtcgccat gagccagcat ggtggtgaca
     9901 cgcaagagcg tattgtcttc cttgagccgg acctcctgat ccgaaaggcg ggtaaagcgc
     9961 gcgatggtct cggccatcgc gatgccccgc ttgcggatca attcatcctg attggcgcgg
    10021 tggcgttcaa gggcggcggc agagtcaagc ccctcggatt tggcatcggt ttcgaaccgc
    10081 gtgacgatgg tgcgcagctc atcaaccgca ccgccaaggc gctgacggta ctgctgggct
    10141 agttccggca attgcgcccc ggccagaccc gaggccagca tccatccggc gaacaccagg
    10201 cgtccaaaaa tcatgcggcc tcccgtcgag ttcatttttc gccgagccgg gcccaattgc
    10261 aagggggcag tccgtcgcgt cgttccacgc atccgggccg acggtgcccc ggcgcggtgt
    10321 cgcaggcatt ccgcgcctgc ggccttatga acccggcagg gtcagcagtc cggccgcgcc
    10381 gtcctcggtt ccgaacaaca ggcgattgcc ggctccatcc caggccagcg cggtgatcgc
    10441 gcccccggcc tcggcgttgc gcaccagcaa ttcggaagca tccgtcagcc ggaccagcat
    10501 caccagccca tcctcgtagc cgatggcaag caccaacgcc ttggggtgga acgcgcaacg
    10561 cgtcacgcgg tgcggccgca cgccgcattc gcgcggagcc ttgcccatcg gacctgtgcc
    10621 cgaaaacggc cagatgatcg ccgcttcggc tcccgacgtg gccagccatt cgccatcgct
    10681 ggaccaggac agcgaacggg ttttcgccgg atagcccgac atgcgcatat gtgtcttctc
    10741 gggcagtcgc cagccatgca gctgattctc ctgcatcgag gaaacgacaa agcggccatc
    10801 gggtgaccag gtgatgtcga gatgcacgcc cttccaatcg agcttttcgg gcgctgcctc
    10861 ggtgttggga aaccacaggc tcgccccgtc gacatgcgac agtgccagtc ggtagccctt
    10921 gggcgcgaag gccaggccac gcgaggagga cggcgcggcg agcgacctga ccgttccctt
    10981 ggcgtcgcgg gcccgcacgg tcctggaaaa actccaggcg gctgagccgt tggggccaag
    11041 cgccacggcg tcgatccagc gaccgggttc ctcggccagc acggtcaggc caccttcggc
    11101 atccgtcagc acaaccctgc cgtcatcacc gccggtaacc atccgcctgc catcggcggc
    11161 ggcgaccagt atggcggcat caggatgggc aggcacggct cgcgcaccct ggccccagcc
    11221 aagccggatg ctgccatcgc ccagcgccag cgctggtgct tctcccagaa acaccgcagc
    11281 ggtgacatgt tcgccagtgg ctattgcgac gacgtgcctc gtcagtgtgt cgttctggcc
    11341 agccatctgc ggtcagaaat cgtaggacag gcgcaggcgg gcacgatgac gctcaaactg
    11401 cgacaggttg agtttctggc ccggttcgtc cttggcatga ccggcaatct gggcgttcca
    11461 ggctgcctgc aggccccaat tgccgctctt caggaagagc gtcgggccaa gataggttgc
    11521 attgccgaga taggttttcc agctgttgcc atcgtaagcc cgctcgtggc gtgcttcgac
    11581 cccgaggaat acacccgggg cgaactgata gctgagcgcg cttgacaacg ccagcgagga
    11641 ggcatggacg ggtgcgcgcg catcccggtc acggcccgac gtcagcccgg cgccgagatt
    11701 gactgtccag aacaggcgct ccgggataat cgcggtatcg gcgatgatcg cgctggcaaa
    11761 gctgaaggcg gtcagccggt tgccggtcgc gtcatccacg gccgtccatt tcggttccat
    11821 ggtgacagcc aggccgatgg gtgagccttc caactggcca ttcaccagct tgccgcgctc
    11881 gatcagccgc atcttgaagc cggccgcaag tgccgagaac gagccacggt tccggttcgg
    11941 gtagaaatag gcgtcgcggg cggtctcgcc tgctgcaagc gcggccaacc ggtcgccgag
    12001 aaccagcgga acatcgttga ggttatgagc gcccaggctg atcgacgggg tgaccgacag
    12061 ccagtccgca agaccatatt cgctttccag cgtcgtggtc agaacctgat agcggccaaa
    12121 ccgcttgccg ttcagtccga tccagccaag cttgacattg gccgggccaa ccttgccggg
    12181 cgtgtcggga ttgtcggaaa agccgtagat gtcgccgccg ttttcccagc ctgacggcgc
    12241 cgcttcggca gatggggccg gattgcgatc cgccgccatc gccgaggtgg cgagcgccag
    12301 catggccgca atgccaattc cttttgtccc cgtcctgacc atgtcccaat atctcctatg
    12361 ccttgcaggc cagaaagccc ttgcggatct cgtcgacctt gagattgcgg ccgatgaaga
    12421 cgatttttga cgtgcgcttc tcgtccgacc gccaatcgcg ttgcaggtca ccgtcgagaa
    12481 tcatgtgcac gccctgatag acgaaacgct tcggttcgtt cggaaaagca agaatgccct
    12541 tcgagcgcaa gatgtccggc ccgtcgcgct gcacgatatc gttgatccac ggcatgaacc
    12601 gctccggatc gacctcgccg tcgatcgtca ccgagatcga ttgcatcgct tcgtcatgat
    12661 gatggtggtg tcccggatcg aggaagtcgg gctcaagatc gagaatccgg tcaagatcga
    12721 aggcgccgcg atcaagcacc tccgacagcg gaatctggca cttcagcgtg cggatcagcc
    12781 gcgcataggg attgatggcg cgaatccgtg cctccacctc gagcagctcc gcctcgctga
    12841 cgaggtccgt cttgttgacc aggatcacat cggcaaaggc aatctggctc ttggcctccg
    12901 gagcgtcctt gagacgggtt gaaagccatt tggcgtcggc aacggtgacg atcgcatcaa
    12961 gccgggcctt ggcctgcaca tcctgatcga cgaagaacgt ctgcgccacg ggcgcagggt
    13021 cggcaagacc ggtggtttcg atgatgatgg cgtcgaactt gccgcgccgt ttcatcagcc
    13081 cctcgacgat acggatgagg tcgccgcgga cggtgcaaca gatgcagccg ttgttcatct
    13141 cgaacacttc ctcatcggcg ccgacgacaa gttcattgtc gatgccgact tcgccgaatt
    13201 cattgacgat cacggcgtag cggcggccgt ggttctcggt gagaatgcgg ttgagcagcg
    13261 tggtcttgcc ggcgccgagg tagccggtca gcacggtgac aggaatcttg tcggacatcc
    13321 attccccctg ccggtcaggg tttcagcgcc ttgaccagcg tcatgatgtt gtggcgcatc
    13381 agatcgatat aggtcgggac cggcccatcc gccagcgaca gggaatcgga atacagcgtt
    13441 ccgccgacgc gggcaccgga atcctgtgcg attcgttcga tcttgcgcgg gtcagtgatg
    13501 ttctcgagaa acacggccgg ggccttgagt gctttcagtt gcctgatgat gcgggcgata
    13561 tctgcggcgg agggctcggc attggtcgaa atgccctgca atgccagcgg cttcagccca
    13621 aaatcgcggg ccagataggc gaaggcatca tgggtggtgg caaacagtct ccggtcatcc
    13681 ggcacggtgg ccaccagagc cttcagctca gtttccagcg catcgagctt gctgcgatag
    13741 gcagcaaggt tggccgcata ggttgccgca cctgccgggt ccaccttggc aagggcgtca
    13801 gccagagtgg tgaccatctg cttcacattg ccaatcgatt gccagatgtg tgggtcctgg
    13861 ttgccgccgt gcttgtggcc atggtcatgg ccgtgggctt gcttggcctc cttcagaaca
    13921 ctcacgccct tcgagagatc cacgaccggg gacttgcttg cgcttgagcg gacgaggcgc
    13981 tccataaagg tatcaaagcc aagaccgttc acgacaatca atgccgcacc ggcgatcgtc
    14041 ttggcgtccg ccggggtcgg cttgtagaca tgggcgtcct ggcccgcgcc aacgatgatc
    14101 ttcacggcga gcctgtcgcc accgattgca cggacgaggt cgccaaggat cgaaaacgag
    14161 gcgaccacag tcactggcga ctgctgggcc gccagcggta gagttcccag aagcgtagcc
    14221 gccgatgcga gtatggagcg acgatcaagc attgtgacag gcatgaaact atcctttcag
    14281 gtgttggctg ggctgtacag tgcgcacgat cccgcctgtc cggccgaagg cgagcgaaag
    14341 tacatagatc ataccggcgg ccagaatgat ggtggggccg gaggcaaggc cgaagtgata
    14401 cgaggcaatc aggccgacca ggctggccag ggttgcgaat gcccaggcca gggcgatcat
    14461 ccgggtcaga tcattgctcc aaagccgcgc cgaggcggcc ggcagtatca atatgccgac
    14521 tgcaagcaat gtcccgaggg catggaaggc cgagacgaga ttgatgacca cgattccgag
    14581 aaagacgagc ggcaaggcgg ctgccgagcg gctgacggag gcaaaaaatc ccgggtcgac
    14641 actatccagc accagcccac ggaacagggc ggacaggctg atcagggtga tcgtggtgac
    14701 cgccagcatc agccatagcg tcggatcgtc gagggccagg accgagccga acagcacacg
    14761 caagacgtca acattggatc cgcgcgccga aagcaacgtc accccggctg cgagcgacag
    14821 gatgtagaag ccagccaaag ccgcgtcttc cttgagggtc gtggtgcgtg ccacgatccc
    14881 cgccaggacg gcgacggcaa agccggccac caatccgcca acggtcatgg caccaagtga
    14941 aaagccggct aataaatatc ccgcagctgc ccctggaagg attgcatggg ccatggcgtc
    15001 gccgatcagg ctcatccggc gaagcatgag aaagacgccg atcggcgcgg cgccacttgc
    15061 cagcacaagg cagccggcca gagcccggcg catgaagccg aattcgacaa aagggccgat
    15121 cagcaggtca taaaatccca tcgtcaggcc cgttcgcaga tgtgggcgtc ggcatcgaag
    15181 gcttcaacca tgcgctgcgc cgcatcaaga ttggtctggg tcaggctgac cagggtcggt
    15241 ccccaggcaa tcgtttcccg ggcgagcaac aaggtctcgg gaaagaccgc gcgcaccagc
    15301 ggcaggtcat gcagaactgc gatcaccgtg cgaccctcgc tatgccagcg acggatcagg
    15361 accaccagat cctcggtggt tttctgatcg atggcattga acggctcatc gagcaggatc
    15421 atcgaggcat cctgcatcag gaggcgggcg aacagcgtgc gctgcatttg cccgcccgac
    15481 agtgtgccaa tggcccggct ctcgaacccc tcaagtccca cggccgacaa ggcgtgctcg
    15541 attctcaggc gtgtggggcg gtcaatcccg ccaaaaagcc ccaccgcatt ccaggcgccc
    15601 atcgagacaa attcatagac cgagatcgga aaggagcgat caatctcaac cacctgcggc
    15661 agataggcaa gatcccggcg gtccttgacg ttccgttcaa ggcggccgcc gagcggccgg
    15721 atgaagccgg ccatggcctt catcagcgtc gacttgcctg cgccgttggg gcctacgatc
    15781 gccaatagcg cgccgtgcgg aatcgtgcct gtcaggtgat ggaccgccgg gtgccgatca
    15841 tagccgagcg tcaaattgtc gaaacggatg gctgaggcca tgatcaggcc tgcacggccc
    15901 agagggtcac cgcccacaat gccatcaccg caaggctagc gaaaatcagg cggctccgcg
    15961 aaccagcggc gatcagcgac agcggggcgg ccagcttggg atgtgcgtcc gggccatgat
    16021 catggccgca gctgcagcca tcgtgatgat gaggattatg cgtgctgttc gacatggcta
    16081 tgttatatta taacgttatg ggcgccgcaa ggggattctg caaatagccg ctgcgggttc
    16141 agaagggatg atactggtag agccaggtct cgctcagggg ctgtccgtcg agacgcagga
    16201 acagccgcat ctccaccggt tccgtgcctt cggcgtggaa gtcgaactgg gcgcgccaat
    16261 gtccggccac gtcattgggc accgcctcgg taaagacgta ggagaagctg ccgcgcgagg
    16321 cggacagcac ggcttcgggc cgcactccgc tcggcagcgc tttcagcggg tcgcccaaga
    16381 actcgaccat gaacttgcgc acgcccttgg ggcgcggctg gccgggctgt ccgccgttgc
    16441 ccatgcgggt ggcgagcacg cgggcgagcg tggtcggata gggctcttcg gcctgccagt
    16501 gcagccggta cttcagggcg aatttgtcgc ccggctggac cggcgcctcc ggaacccagg
    16561 ccgcaacgac attgtcgtgg atttcgtcgt cggtggggat ctcgatcagc gtgacggacc
    16621 ccttgcccca gttgccgacc ggttccaccc acaggctcgg ccggcgctcg taatagacgc
    16681 catcctgata gtgatcgaag gcacggtcgc gctggaggag gccgaagccg cgcgggttcg
    16741 tgtcgccgaa ggacgaggcg agggtgcgcg gcggattgtt gagcgggcgc cagatgcgct
    16801 cgccgctgcc ggtccacagg gcgagcccat cggaatcgtg aacctccggg cgccagtctg
    16861 ctgcggtagg tttggcggtt tcggagaacc agtacatcga ggtcagcggc gcaatgccga
    16921 agcgcgccac cgccttgcgc acgaacaggc gcttgtcgat gtccatggtc acgccccggc
    16981 cgcgcttcat gtcgaagcgg aacgcgccgg cgacgctcgg gctgtcgagc agggcataga
    17041 cggtgacatg tccggcgctc tgcccctgtt caagccagat atgggtgaaa tcgggaaatt
    17101 cttccggcgt tccgatggcg gaatcgagca tcaggccgcg ggccgagagc ccatactgga
    17161 actgatcgcc gatggcgcgg aaataggaag cgccgagaaa ggccacccaa tcgtttttct
    17221 tccagtccag tgtgccgtca acagcttcct gaaagcggaa accggcaaag cccgcgcctt
    17281 tcggcagctg cttggccggg ctgtccgccg gcatgtcgaa ataatcctcg cgatagatga
    17341 tttcgcgcgc cttgccaccg tccaccacat gcatgcgcac cggtttgacg aaatagcggc
    17401 cgagatggaa gaaggtcacc ggaaagcgac gctgctcctg cgggcgtccc cagacggcaa
    17461 aatcggtctt gaacctgagc ttgccatgcg cgtcatagtc aatcctttcg agcacatcgg
    17521 gtgccggacg gggtggggga acataggcgg acgccgccgc cagacgcgcc cgctcgacca
    17581 gcgcatcaaa gctgaaggtt gcggccggtc cgaggtcaag cccgggttcc tgggcggata
    17641 tctgccttga gggcagcgca gcggcggcaa gaactgcgaa aagtgtgcga cgatccatgg
    17701 cggttccgaa agggtgcggg cggcagtccg atccgttact ggtagatcgg ctcgatcttc
    17761 agcgcgccct gcaggaaccg gaatgtggcc cagccgccgg ccgcgataca aacggccatg
    17821 atcccccagg tgacctcgcg gccaagctgg aacagcccgc caagcgccca gccagccgcc
    17881 aataaggcgc cgatcacctc gacgccgacg agaatcgtca ccgagacgat ggtcaggaga
    17941 ttgctggcat tgaggcgctt ggccatggcg aaatccactt caggcggcaa cagccggacg
    18001 ggcagacgtt gatcacacgt cgctacagga aagcgccgga tgcgacaatc ccctgtcaac
    18061 tgctggtcag ataccccatc agcaacgcag ctccaagcaa cgtaaccgcc agcgctgccg
    18121 cgcattcaac tgcggcagtc agccaggcaa gacgctcact gcgttcggcc agccgccgtg
    18181 tggcgccttt ggccagcacg gcgcccaccg caaaacccgc cgttgtcagg ccggtgccga
    18241 ccgccatggc aaacaccgca gcgatgccga ctcccaaggc gccgaccgag agcgcgaatg
    18301 ccaacaggat gatcgctccc gagcaaggcc gcgagcctgc ggccaccacg actgcaacca
    18361 gatcacgcca ggactttgtc ccggcgagat cggtagcatc gggcaaatgc acatgggtgc
    18421 aggcggcgcc atggtcatgg tggtgaccat ggtgctggtg ggtatggctt ccgatgccta
    18481 gtccgctgcc gcaatgcgga cagggggcca gtgactggtc cggggcctgc ttgaaaaaag
    18541 ccagaaaggc gcggcccttg cgccagagca gcgacagacc gagcaagagg atcgcgctga
    18601 acgagatgat ctcgatccag gcgacggact ggtcgacctg acgggccgtt gcgccaagca
    18661 ggctgaccgc cggcaccacc agcgtgatgg cgacaacagc ctgcaacaac gccgctgcca
    18721 gcgccaccag cacggcgcgt ttgagcacat cctcgttggc aaacacatag gacgagacca
    18781 cggccttgcc atggccgggg ccggcagcgt ggaacacccc ataaaggaaa gccaccgaga
    18841 tcaacgccca ggcgccggcc ccctcgcgcg ttgcacgcaa ggcggtgatc atcgcccggt
    18901 tgaactcgac ctgtttggcg aggatccacg ccaccagccc gccaaacagg
//
LOCUS       DAOMXG010000030        18605 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_1723_length_18605_cov_9.172722, whole genome shotgun sequence.
ACCESSION   DAOMXG010000030 DAOMXG010000000
VERSION     DAOMXG010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 18605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18605
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1723_length_18605_cov_9.172722"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="PLQ11_03565"
     CDS             <1..956
                     /locus_tag="PLQ11_03565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRK24013.1"
                     /translation="AFNEGPNIDALLLRLAPILDRAAQSWEIVFVDDGSRDDTLARIR
                     ALAGRDSRIRAVSFSRNFGKEIALAAGLDHSRGRAVIIIDADLQHPPETIETFVAKWR
                     EGFDMVYGQRVDRSTDGPVRRWLSGKFYRVFERFGETALPDGAGDYRLIDRKVVDALT
                     RMGERARFSKGLFAWVGFKSIGIPFEVADRAHGMSKWSFRKLMGFALDGLASFSTLPL
                     KVWTYIGSIISTFAICYALFFAIKTLVFGADVPGFPSLIVSVMFFSGIQLVSLGVIGE
                     YVGRIFAEVKRRPLYIVAEEIGPDCEMPGESGGQAGRARVG"
     gene            complement(958..1614)
                     /locus_tag="PLQ11_03570"
     CDS             complement(958..1614)
                     /locus_tag="PLQ11_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HRK24014.1"
                     /translation="MTTHAYAFDAYGTLFDVHAAIARHRGALGTDAEAFSALWRAKQL
                     EYSWTRTLMGRTRDFWQLTQDALDFCLARFPGVDPALRQPLLESYFRLDAFADARPTL
                     LRLRADGARCALFTNGTRAMAESAAASAGLADCLEMIVSVEAAGRFKTVPEVYALVAG
                     RLGLPMAAVTLVSSNRWDIAGAAACGMPGIWVNRTGQPDEYPDLAPVRIITRLDELSC
                     "
     gene            1821..2243
                     /locus_tag="PLQ11_03575"
     CDS             1821..2243
                     /locus_tag="PLQ11_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24015.1"
                     /translation="MTTTRRALLGATAAFAAVPAAFAAPSALSSMTAPSAAAAGASEI
                     RHLWDEVIDLSIRLGGHPTPLVAQETGLPGWMYVSGEANELGNARYDRLVKILHATPR
                     SNEDLAIIARAANHCDIQTGPRTYAAARLADAAMTMAA"
     gene            2380..4197
                     /locus_tag="PLQ11_03580"
     CDS             2380..4197
                     /locus_tag="PLQ11_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase subunit beta"
                     /protein_id="HRK24016.1"
                     /translation="MSTLPLASDFPAASRDQWLKLVEGVLKGADFDKKLVGRTYDGLT
                     ISPMSQRRKAATPVTRAGAGAAWKLSQRIELPDPARANAQALEDLMNGANALTLVFAD
                     APGAHGFGLKDASRETLTRVLGQIHLDMGVTLDLDMGGQSREAGKVIAEIARAKGLKP
                     DQIDVKFGYNPIGRIARGFGSPDEWADIAPMVAAVVKDHVAQGLKGPFMVGDGRLVHA
                     AGGSEAQELAFALASAVTYLRALESAGMDLSVARDAIYMRLAVDADQFASIAKLRAAR
                     RLWARIEQACGLTPKPGFLVAETAWRMLTRNDPHVNILRNTVAVFAAAIGGADLISCL
                     PFTAALGLPDATARRLARNTQVVLAEEANLYRIADPAAGAGSVEDLTEAMAEKAWVLF
                     QEIEAAGGIAKALPAFADKVTAVRAQRMKAVATRKDPLTGASEFPNVHEASVEVLAPM
                     PPLADKGLLVPMRLAEPFEALRDAACRSKTRPRVFLANLGAIPDFTARTIFAKNFFEA
                     GGIEAPTNDGFADTAALVAAYRASGARIACLCSSDAVYAAEAVAAARALGAAGATLYL
                     AGRPGELESALREAGLSRFVFVGCDAVATLGEALRLAQA"
     gene            4335..4916
                     /locus_tag="PLQ11_03585"
     CDS             4335..4916
                     /locus_tag="PLQ11_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HRK24017.1"
                     /translation="MQATSYAPMIASLVEGRDRSALVRKTVLVLAGTLALALSAKVQV
                     PFYPVPMTMQTLVVLVIGATFGLRLGLATIGLYLLEGFAGLPVFATGAGPAYMAGPTG
                     GYLAGFVAAVGLMGWGASKGYDRSFGGSLALMSVGHLVILALGFLWLSTLIGPDKAFA
                     AGIAPFAWATLFKTLLAAGLVPALWSLIQRYRR"
     gene            5043..5579
                     /locus_tag="PLQ11_03590"
     CDS             5043..5579
                     /locus_tag="PLQ11_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24018.1"
                     /translation="MLRYIRAGGVTTFVISSLAALVVTGFGVWYSSLTSGEIAERLIL
                     WFGLAFIAVAPVALILMPLVHLILGFGERPAGRLFAMIGGVLGALVAGYALFRFRGVL
                     FPSTGIAFLAIPAMVIGATLLGILGGFLFERLARRKRSEATILPPVKPASGTPAQSDE
                     GRVSEPGHPDVRPDRKML"
     gene            5594..7747
                     /gene="scpA"
                     /locus_tag="PLQ11_03595"
     CDS             5594..7747
                     /gene="scpA"
                     /locus_tag="PLQ11_03595"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HRK24019.1"
                     /translation="MTRIPDFSSLSYGQAPSAPAPTAGGDWQTPEGIAIKPVYGEEDL
                     AGLDFLDTYPGIAPYLRGPYPTMYVTNPWTVRQYAGFSTAEDSNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDHPRVSGDVGMAGVAIDSIYDMRTLFDGIPLDQMSVSMTMNGAV
                     LPILSLYIVAAEEQGVAPAKLSGTIQNDILKEFMVRNTYIYPPKGSMRIISDIFAYTA
                     QHMPKFNSISISGYHMQEAGATQDLELAYTLADGVEYIRAGIKAGLPVDKFAPRLSFF
                     WAIGMNFFMEVAKMRAARLLWAKLVKQFDPKDQRSLSLRTHCQTSGWSLTAQDVSNNV
                     VRTCVEAMAATQGHTQSLHTNALDEALALPTDFSARIARNTQIFLQQEAGTNRFIDPW
                     GGSAFIEKLTGDLAARAWDHIQEVEKLGGMAKAIEAGIPKLRIEEAAARTQARIDSGQ
                     QTIVGVNKYRLDNEPPVDVLKVDNAAVRASQIAKLKRLRAERDQGAVDNALAALEAAA
                     RSGEGNLLALGVEAARAKATVGEISLALEKAWGRHVAEIRAISGVYKREVGVNSEMIT
                     RVHDKVAAFEEADGRRPRILVAKLGQDGHDRGQKVIASAFADLGFDVDIGPLFATPEE
                     AARQAVENDVHIIGVSSLAAGHLTLVPAVKAALEKEGRGDIMIVAGGVIPPQDYEALY
                     KAGAAAIFPPGTAIADAAGKLLDDLSDRLGYRQKG"
     gene            complement(7753..8793)
                     /locus_tag="PLQ11_03600"
     CDS             complement(7753..8793)
                     /locus_tag="PLQ11_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSU2403 family nucleotidyltransferase fold
                     protein"
                     /protein_id="HRK24020.1"
                     /translation="MIERLSPLAQTTYVDLVAALQDEAVAAVRGTPSRVTRGKRAYWY
                     DMYRIGASVRRSYIGEDTPELRARLERERPGADDLERQRYRARLIRVLRAEGFMGTDA
                     ATGSLLGAMQKAGVFRLGGVLVGTAAFRLYQGELGLRYRLDDTAQTNDIDIASFERLS
                     LALEDVVSESLQNVLADFEFDPVPSLDPGKVWRWRQTRSNLLVEFLTPSFDADEGIRP
                     LAALGVEAQALHYLNYLIAEPVAAALTYRSGVLIHIPRPERFAIHKLIVADRRREGVD
                     SLKAVKDRAQAAFLINALSEDRPDDLAEAYEDAMARGSRWRERIAATLRRMPEATARL
                     EASGISSLTRTE"
     gene            complement(9515..10219)
                     /locus_tag="PLQ11_03605"
     CDS             complement(9515..10219)
                     /locus_tag="PLQ11_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006940043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HRK24021.1"
                     /translation="MIHVRKSAERGHANFGWLDSYHSFSFGQYYDPQFMGFGPLRVIN
                     DDRVAGGGGFPMHPHDNMEIVTYMLSGSLAHKDSLGSGSTIRRGDVQRMTAGTGIRHS
                     EFNASATDEAHFLQIWILPEVRGLAPSYEEKHLGDAGRRGRLQLIASRGARDGSVHIN
                     QDVDLYASILNEGEKLAHTFRPGRIGWLQVALGSVAVNGSELKQGDAVAMADMDAITI
                     EGRSKGAEILLFDMAR"
     gene            10340..10855
                     /locus_tag="PLQ11_03610"
     CDS             10340..10855
                     /locus_tag="PLQ11_03610"
                     /inference="COORDINATES: protein motif:HMM:NF012982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HRK24022.1"
                     /translation="MIIPLIIAAVDTRPEWGQILLSCCPGRADQDHDLDHDIGVLKRH
                     KVAAVITLNALEELDEMGVGYLGRAVHGAGMEWFYLPIEDYCRPDADWERDWLMTGPQ
                     IRGMIRDGKNIHIHCRAGCGRSGTLVARLLVELGICDPEEAIRRTRFARPCAIERPEQ
                     EDVVREARPIH"
     gene            11040..12032
                     /gene="meaB"
                     /locus_tag="PLQ11_03615"
     CDS             11040..12032
                     /gene="meaB"
                     /locus_tag="PLQ11_03615"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011442421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HRK24023.1"
                     /translation="MRTSPAPGPVDDLANRLRAGDRAALARAITLVESKRLDHQAQAR
                     ALVQRLLPFTGKAVRLGITGVPGVGKSTTIDVLGMRLISEGHKVAVLAVDPTSSRTGG
                     AILGDKTRMARLSMHESAFIRPSPAGQTLGGVAARTREAMLLCEAAGFDVVLVETVGI
                     GQSETAVADMTDFFLVLLLPGGGDELQGIKKGVIELADMIAINKADGESLGRARSTAM
                     EYSAALNLLTPDSARWKPPVILISGLNDEGLDGLWSNVSQHRALLGASGDLAAKRARQ
                     QVKWMWTMLDDRFRSRLRADATLRARLPEIEEAVFTGALSATLGADEIAAMVGL"
     gene            12029..12640
                     /locus_tag="PLQ11_03620"
     CDS             12029..12640
                     /locus_tag="PLQ11_03620"
                     /inference="COORDINATES: protein motif:HMM:NF013624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C15"
                     /protein_id="HRK24024.1"
                     /translation="MSNRVLLVTGFGPFPGVPRNPAERTARAIAADGGLRRIGLTTHC
                     AIFHTSYAALEGEAGPLLAQVQPAAILMIGVASRRRHLSVELNARNRRALLFPDAAGQ
                     RSARLAINPSMAFLSKGRAPFAACLRAGHAAGLDTRLSTSAGTYLCNYSYWLMLEATD
                     ARIPCLFLHIPKLRGPWHFQRLVRAGVAMARLLVQDARWRSNR"
     gene            complement(12629..13645)
                     /locus_tag="PLQ11_03625"
     CDS             complement(12629..13645)
                     /locus_tag="PLQ11_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009494005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HRK24025.1"
                     /translation="MSEPVLVEVTRGGIVESAHRGTLVVADADGRIVAALGDGERRAF
                     PRSAVKALQALVLMESGAADRFGLDDADIALACASHSGEEVHATQALSMLKKAGQTAT
                     CLECGAHWPMREEAARALAAAGRKPSALHNNCSGKHAGFICAAVTMGEDPKGYVRPDH
                     PLQRAVTAALETSLGLDLSDAPRGVDGCSIPTYAVPLAALARGFARFGTGMGYGRERA
                     RAAARIRQAVAAAPFMVAGSNRFDTTIMRALGQRAFVKTGAEGVYCAALPEQGLGIAL
                     KIDDGAKRASAAVMASLIAACLPLDAAQGTVVAAAMDTPLVNWNGIEVGRVRPSAAVR
                     ALSV"
     gene            13737..14183
                     /locus_tag="PLQ11_03630"
     CDS             13737..14183
                     /locus_tag="PLQ11_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004118663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HRK24026.1"
                     /translation="MHFAHTMVRVTDLDASLKFYCGLLGLVETRRTESEKGRFTLIFL
                     AAPEQAALARDNRWPEVELTYNWDPDPQPYGIGRAFGHLAFRVDDIYAICEKLQKAGV
                     AINRPPRDGRMAFVRSPDNISIELIQKGDALPLREPWTSMANDGWW"
     gene            complement(14196..15413)
                     /locus_tag="PLQ11_03635"
     CDS             complement(14196..15413)
                     /locus_tag="PLQ11_03635"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004674947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HRK24027.1"
                     /translation="MPRVLLIVCDSVGVGGAPDAAAYGDAGADTLGHIAEACAAAKGD
                     RDGLRAGPLGVPNLVALGLGEACRIATGRVPAGLQGPHRTGAAGAAIETSRGKDTPSG
                     HWEIAGVPVTFDWGYFPHSVPTFPADLIAAFLKETGLGGILGDCHASGTEIIERLGEE
                     HIRTGWPICYTSVDSVLQIAAHEQHFGLDRLYAICGVMRRLTRPLNIGRVIARPFLGT
                     ASGNFARTPNRKDWALAPPQPTLLDRLEAAGRPVVSVGKIGDIFSHKATGREVKGPGN
                     MANLDLALREFSILKEGGLVFANLVDFDSEAGHRRDVPLYAHLLEQFDARLPDIHAVL
                     RPGDVCIITADHGNDPTWGGTDHTREAVPVLAFGPDMPPRCIGLRQSFADISQSVLGH
                     LRVPRGPVGTSFL"
     gene            complement(15417..16745)
                     /gene="deoA"
                     /locus_tag="PLQ11_03640"
     CDS             complement(15417..16745)
                     /gene="deoA"
                     /locus_tag="PLQ11_03640"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009466924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HRK24028.1"
                     /translation="MLPQEIIRTKRDGGTLTATEISDFIAGLVDGRVTDMQASAFAMA
                     VFFNGMSRDEAVALTCAMRDSGEVLDWSRESLPGPVLDKHSTGGVGDNVSLLLAPAVA
                     ACGGFVPMISGRGLGHTGGTLDKLDSIPGYVSQPDSGRFRAVVRTVGCAIIGQTERLA
                     PADKKLYAIRDVTATVESVPLITASILSKKLAAGLQGLAMDVKTGSGAFMPTLEKARA
                     LARSIVAVANGAGLKTHALITDMNEALAPAAGNAVEVRNAVDNLTGARRDARLIEVTT
                     ALGAEMLLVGALADDLDDARARIAGALDSGAAAETFGRMVAALGGPVDFVQHAERYLP
                     RAPVMRPVPAGRSGRVMAVDTRALGLAVVMLGGGRTRAADRIDPGVGLTDLVALDPIV
                     AADTPLAIVHARDEAAAEAAVAAVRAAYHIGQHTERPALLVERITAADGE"
     gene            complement(16885..17655)
                     /gene="deoC"
                     /locus_tag="PLQ11_03645"
     CDS             complement(16885..17655)
                     /gene="deoC"
                     /locus_tag="PLQ11_03645"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009494565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HRK24029.1"
                     /translation="MSMQADLSLARRAMALMDLTELGDGADEAAVRHLCATARGGNGI
                     PAVAAVCVWPRHVRLARQELAGIRVATVVNFPTGGHALADVQHETRMAMSDGADEIDF
                     VLPYRMFLAGGAGVCDLMLRLVRGDIPFGKRLKVILETGAFPDAASIRRAADLTIAAG
                     ADFIKTSTGKIATGATPEAARVMLEAIRAAGRPVGLKPSGGIRTLADARLYLDLADSV
                     MGPGWARPETFRFGASGLHASLAAVIKSDAPPTAQLRY"
     gene            complement(17652..18446)
                     /locus_tag="PLQ11_03650"
     CDS             complement(17652..18446)
                     /locus_tag="PLQ11_03650"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014129223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HRK24030.1"
                     /translation="MSALQTIIGRAGRQPVEAGLVLGSGLGPLADRVEEAVAIAYGDL
                     EGFPGGGVSGHAARLVIGRLGGRRVAVFCGREHYYEHGNAAAMRPALETLVGLGAKTL
                     LLTNAAGSLRAEVGPTSVVVHSDYVNWSGRNPLIGEAGDARFVDMRDCYDPGLRARLR
                     AVAERQGLPYREGVYFWFSGPTFETPAEIRAMKALGADLVGMSTVPEVIIARRLGLRV
                     AALSMVTNLAAGFAGADISHHDTKTVAARGAERMAGLIVGLLEDGL"
     gene            complement(18443..>18605)
                     /locus_tag="PLQ11_03655"
     CDS             complement(18443..>18605)
                     /locus_tag="PLQ11_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HRK24031.1"
                     /translation="DGQLCTPCGACRQRIREFARPNCPIHIAGPEGLRRSFTLDELLP
                     HAFGPENLG"
BASE COUNT         3299 a         6103 c         5917 g         3286 t
ORIGIN      
        1 ctgccttcaa cgaggggccg aatatcgacg ccctgttgct gcggctggcg cctatcctcg
       61 atcgcgccgc gcaaagctgg gaaatcgtgt tcgttgacga tggcagccgc gacgacacgc
      121 tggcgcgcat ccgggcgctg gcggggcgcg acagccgtat ccgcgcggta tcgttctcgc
      181 gcaatttcgg caaggaaatc gccctcgccg cagggcttga ccattcgcgc ggccgcgccg
      241 tcatcatcat cgacgccgac ctgcaacacc cccccgagac aatcgagacc tttgtcgcca
      301 agtggcgcga gggctttgac atggtctacg gccagcgtgt cgaccgttcg accgacggac
      361 cggtgcggcg ctggctgtcg ggcaagttct acagggtgtt cgagcgcttc ggcgagaccg
      421 ccctgcccga tggcgccggc gactatcggc tgatcgaccg caaggtggtc gatgcgctga
      481 cgcgcatggg cgaacgggca cggttctcga aagggctgtt cgcctgggtc gggttcaagt
      541 ccatcggtat cccgttcgag gtggcggatc gcgcgcatgg catgtcgaaa tggagcttcc
      601 gcaagctgat gggctttgcg ctcgacggtc tggcctcgtt ctcgacactg ccgctcaagg
      661 tctggaccta tatcggctcg atcatctcga ccttcgcgat ctgttatgcg ctgttcttcg
      721 cgatcaagac cctggtgttc ggggcggatg ttccgggctt tccctcgctg atcgtgtcgg
      781 tgatgttctt ctcagggatc cagctggtct cgctgggcgt catcggcgaa tatgtcggcc
      841 gcatcttcgc cgaagtgaaa cgccgcccgc tctacatcgt cgccgaggag attggtccgg
      901 actgcgagat gccgggtgaa agcggcgggc aggcgggcag agcccgcgtc gggtaattca
      961 gcacgacagt tcgtcgagcc gggtgatgat gcgcaccggc gcaagatcgg gatattcatc
     1021 cggctggccg gtgcggttga cccagatgcc aggcatgccg caggcggccg cgccggcaat
     1081 atcccagcgg ttcgaggaca caagcgtgac cgccgccatc ggcagtccaa ggcggccggc
     1141 aaccagcgca tagacctcgg gaaccgtctt gaaccggccg gcggcctcga ccgagacgat
     1201 catttccaga caatcggcaa gcccggcgct ggcggctgcc gattccgcca tcgcgcgggt
     1261 tccgttggtg aacagggcgc agcgcgcacc gtccgcgcgc aggcgcagca gcgtcggccg
     1321 cgcatcggcg aaggcatcga gccggaaata tgattccagc agcggctggc gcagcgctgg
     1381 atcaacgcca ggaaaccgcg ccaggcagaa atccagcgca tcctgcgtca attgccagaa
     1441 atcccgtgtc cgccccatca gggtacgggt ccaggaatat tcaagctgct tggcacgcca
     1501 cagcgccgaa aacgcttcgg catctgtccc cagcgcgccc ctgtggcgcg cgattgcggc
     1561 atgcacatcg aacagggtgc cataggcatc gaatgcatag gcatgcgtgg tcatgcttgg
     1621 ggcctccggc gccattgttc catgcagcat ccgcgccagc aacccggaca aaaagaagtg
     1681 atcggcgggc cttcaactcc gcattattca gatagcgttc aactcagaat gagcataaca
     1741 tacttcgcgt atcgtgagga gcgatgccgc atccggatgt cgagacccat cagatgcctg
     1801 aacccgagag gacctctgcc atgaccacca cccgccgtgc cctgctgggc gccaccgccg
     1861 cctttgccgc tgttccggcc gctttcgccg cgccgtcggc cctgtcgtcg atgaccgctc
     1921 cgtccgctgc tgccgccggc gccagcgaaa tccgtcacct gtgggacgag gtgattgacc
     1981 tgtcgatccg cctcggcggc catcccaccc cgctggttgc ccaggaaacc ggcctgccag
     2041 gctggatgta tgtgtcaggc gaggccaacg agcttggcaa cgcccgctat gaccggctgg
     2101 tgaagatcct gcatgccacc ccgcgctcga acgaagacct ggcgatcatc gcccgcgccg
     2161 ccaatcattg cgacattcag accggtccgc gcacctatgc agctgcgcgc cttgccgatg
     2221 ccgcgatgac catggcagcc tgacatcagg cgagtagcca aacgcatcgc catcctggcc
     2281 cccgcccgca ggcgggggct ttgctttggt gcggactacc aaccaaatgc gcaatgcagg
     2341 catcttttca gggccctaag tcgggcgtag aaggtgtgca tgagcacact gccgcttgcc
     2401 tccgatttcc ccgccgcctc gcgcgatcaa tggctcaagc tggtcgaagg cgtcctcaag
     2461 ggcgcggact tcgacaagaa gctggtcggc cgcacctacg atggcctgac catttcgccg
     2521 atgtcgcagc gccgcaaggc tgcaactccg gtgacgcgcg ccggagccgg cgctgcgtgg
     2581 aagctcagcc agcggattga acttcccgat cccgcaaggg cgaatgcgca agcgctcgag
     2641 gatctgatga acggggccaa tgccctgacg ctggtgttcg ccgatgcgcc cggcgcgcat
     2701 ggtttcggcc tcaaggatgc ctcgcgcgag acgttgacgc gcgtgctcgg gcagatccac
     2761 ctcgacatgg gggtgacgct cgatctcgac atgggcggcc agtcgcgcga ggccggcaag
     2821 gtgattgccg agattgcccg ggccaagggc ctcaaaccgg atcagatcga cgtcaagttc
     2881 ggctataatc cgatcggccg gatcgcccgc gggttcggca gtccggacga gtgggctgat
     2941 attgccccga tggtggccgc cgtcgtcaag gatcacgtcg cccagggcct gaaaggcccg
     3001 ttcatggtcg gcgacgggcg cctcgtccat gcggcaggcg gttcggaagc gcaggaactg
     3061 gccttcgcgc tggccagtgc ggtgacctat ctccgggcgc ttgagagcgc cggcatggat
     3121 ctttccgttg cccgtgatgc gatctacatg cggctggctg tcgatgccga ccagtttgcc
     3181 tcgatcgcca agctgcgtgc cgcacggcgg ctgtgggcgc gcatcgagca ggcgtgtggt
     3241 cttacgccaa agccggggtt ccttgtcgcc gaaacggcat ggcgcatgct gacgcgcaac
     3301 gatccgcatg tgaacatcct gcgcaacacg gtggcggtgt tcgccgctgc catcggtggc
     3361 gctgatctga tctcctgcct gcccttcacc gcagcactcg gcctgccgga tgcaaccgcg
     3421 cggcggctgg cccgcaacac ccaggtggtt ctggcggaag aagccaacct ctaccgcatc
     3481 gccgatccgg ccgccggggc cggctcagtc gaggatctga cggaagcgat ggcagaaaag
     3541 gcgtgggtcc tgttccagga gatcgaggcg gcgggcggca tcgccaaggc cctgccggct
     3601 tttgccgaca aggtcacggc cgtgcgtgca cagcggatga aggcggttgc aacccgcaag
     3661 gacccgctga caggcgcctc ggaattcccc aatgttcacg aagcatcggt cgaggtgctg
     3721 gcgccgatgc cgccgctggc ggacaagggc ctgctggtgc cgatgcgact ggccgaacct
     3781 tttgaagctc tgcgcgatgc cgcatgccgc agcaagacac ggccgagggt gtttctcgcc
     3841 aatctcgggg cgatcccgga tttcaccgcg cgcaccatct ttgcgaagaa cttcttcgag
     3901 gctggcggca tcgaggcgcc gaccaatgac ggttttgccg atacggcggc attggttgcc
     3961 gcctatcgcg cttctggcgc ccgcatcgcc tgcctctgct cgtcagacgc ggtttatgca
     4021 gccgaggccg tggccgccgc cagagccctt ggcgctgccg gggcgacact ctaccttgcc
     4081 ggccggccgg gcgagcttga gtcggcgttg cgcgaggctg gcctcagccg cttcgtattc
     4141 gttggctgcg atgcggtggc gacattgggc gaggcgctgc ggctggcaca ggcatagcga
     4201 tttcaagcga aacggattcc ggttcgcata aagaaagcgc gattagacag tttggacttt
     4261 ttggggggct tgccgccgag gcaaggacct cctaccacaa gcgtcacagg atcacattca
     4321 agaggacggt ttccatgcag gccacttcct acgctccgat gatcgccagc ctggtggaag
     4381 ggcgcgaccg cagcgctctt gtccgcaaga cggttcttgt gctggcgggc acgctggcgc
     4441 tggcgctgtc ggccaaggtg caggtgccgt tctatccggt gccgatgacc atgcagacgc
     4501 tggtggtgct ggtgatcggg gcgactttcg gcctgcgtct ggggctggcg acgatcggcc
     4561 tctatcttct cgaaggtttc gccggcctgc cggtctttgc aacgggcgct ggcccggcct
     4621 acatggccgg cccgaccggc ggctatctcg ccgggttcgt ggcagccgtc ggcctgatgg
     4681 gctggggcgc cagcaagggc tatgatcgca gcttcggcgg ttcgctggca ctgatgtcgg
     4741 tgggccatct ggtgatcctc gccctgggat ttctgtggct gtcgacgctg atcggcccgg
     4801 acaaggcgtt tgccgctggc atcgcgccct tcgcctgggc gacattgttc aagacgctgc
     4861 tggcggccgg ccttgttccg gcgctgtggt cgctgatcca gcgctatcgc cgctgacaca
     4921 gccgcctgtc tggcaggatc aacgcccggg gcaatcctcc gggcgttttt cgtcttgcag
     4981 acccggtttg gccgcacccg cagggatgct ataagatcgt cgcatcgaat gggagagacg
     5041 cgatgttgcg ttacatccgc gccggaggcg tgaccacctt tgtcatttcg tcgttggcgg
     5101 cactggtcgt gaccggattc ggcgtctggt attccagcct gacgtcgggc gagatcgccg
     5161 agcgcctgat cctgtggttt ggccttgcct tcattgctgt ggcgccggtg gcgctgatcc
     5221 tgatgccgtt ggtccatctg atcctcggct tcggcgagcg accggcggga cggctgtttg
     5281 cgatgatcgg cggcgttctc ggggcgctgg tggccggcta cgcgctgttc cgctttcgcg
     5341 gcgtgctgtt cccatcgacg ggtatcgcct ttcttgccat accggcgatg gtgatcggcg
     5401 caacgctgct tggcattctc ggcggatttc tgttcgagcg gctggcgcgg cgcaaacgca
     5461 gcgaggcaac catcctgcca ccggtcaagc cggcgtccgg aacccctgct cagtccgacg
     5521 aaggacgtgt ttccgaaccc ggccaccccg atgtaagacc tgatcggaaa atgctctgat
     5581 ccgcgaggcc gccatgaccc gtatcccgga tttctcttcg ctatcctacg gacaagcccc
     5641 cagcgcgccg gcgccgactg ccggaggaga ctggcagaca ccggaaggca tcgcgatcaa
     5701 gcctgtctat ggcgaggaag accttgccgg gctcgatttt ctcgacactt accccggcat
     5761 cgcgccctat ctgcgcgggc cttacccgac aatgtatgtg accaacccgt ggacggtgcg
     5821 ccagtatgcc ggattctcca cggcggagga ttccaacgcc ttctatcgtc gcaacctcgc
     5881 ggctgggcag aagggccttt cggtcgcctt cgatcttgcc acccatcgcg gctatgacag
     5941 cgaccatccg cgcgtttccg gcgatgtggg aatggcgggc gtcgccatcg attccatcta
     6001 cgacatgcgc accctgtttg acggaattcc gctcgaccag atgtcggtgt cgatgaccat
     6061 gaacggcgca gtgctgccga tcctgtcgct ctacatcgtc gcggccgaag aacagggcgt
     6121 ggcgccggcc aagctgtcgg gcacgatcca gaacgacatt ctcaaggaat tcatggtccg
     6181 caatacctac atctacccgc ccaagggatc gatgcggatc atctcggaca tcttcgccta
     6241 taccgcccag cacatgccga agttcaactc gatctcgatc agcggctacc acatgcagga
     6301 agccggggcg acgcaggacc tcgaactcgc ctatacgctg gccgatggcg tcgaatatat
     6361 ccgcgccggc atcaaggccg gactgccggt ggacaaattc gcgccacggc tcagcttctt
     6421 ctgggccatc ggcatgaatt tcttcatgga agtggccaag atgcgcgccg cgcgcctgct
     6481 gtgggccaag ctggtcaagc agttcgaccc gaaggaccag cgctcgctct ccctgcgcac
     6541 ccattgccag acctcgggct ggtcgctgac ggcgcaggat gtctccaaca atgtcgtgcg
     6601 cacctgcgtc gaggcgatgg cggcaacgca gggccacacc cagtcgctgc ataccaacgc
     6661 ccttgacgag gcgctggcgc tgccgaccga cttttccgcc cgcatcgccc gcaacacgca
     6721 gattttcctg cagcaggagg ccggcaccaa ccgcttcatc gatccgtggg gcggttcggc
     6781 cttcatcgaa aagctgaccg gagatcttgc cgcgcgtgcc tgggaccata ttcaggaagt
     6841 ggaaaagctg ggcggcatgg ccaaggccat cgaggccggc atcccgaaac tcaggatcga
     6901 ggaagccgca gcccgcaccc aggcgcgcat cgattccggc cagcagacca tcgtcggcgt
     6961 caacaagtat cggctcgaca acgagccgcc ggtggatgtg ctgaaggtcg acaatgccgc
     7021 cgtgcgcgcc agccagatcg ccaagctcaa gcgcctcagg gccgagcgcg accagggcgc
     7081 tgtggataac gcgctggctg cgctcgaagc cgctgcccgc tccggcgagg gcaacctgct
     7141 ggcgctgggc gtcgaggcgg cacgggccaa ggcgactgtg ggcgagatat cactggcgct
     7201 ggaaaaggcc tgggggcggc atgtcgcgga aattcgcgcc atttccggcg tctacaagcg
     7261 ggaggtcggc gtgaattccg aaatgatcac ccgcgttcac gacaaggtcg ccgcgttcga
     7321 ggaggccgac gggcgccgcc cgcgcatcct cgtcgccaag ctcggtcagg acggtcacga
     7381 ccgcggccag aaggtgatcg cctcggcctt tgccgatctc ggctttgatg tcgacatcgg
     7441 gccgctgttc gcaaccccgg aagaagccgc ccgacaggct gtggaaaacg atgtgcatat
     7501 catcggcgtc tcgtcgctgg cggcggggca tctgacgctg gtgccggcgg ttaaggccgc
     7561 gctggaaaaa gaagggcgcg gcgatatcat gatcgtcgcc ggcggcgtca tcccgccgca
     7621 ggattacgag gcgctctaca aggccggcgc cgccgccatc ttcccgcccg gcacagccat
     7681 cgccgatgcc gccggaaaat tgctcgacga tctgagtgac aggctggggt atcggcagaa
     7741 ggggtgagtc gattattccg ttcgcgtcaa tgaactgatc cccgatgcct ccagccgcgc
     7801 cgtcgcttcc ggcatgcgtc tgagggtcgc tgcaatccgc tcccgccagc gggagccgcg
     7861 cgccatggcg tcttcatagg cttctgccaa atcgtcgggg cgatcctcgg acagcgcatt
     7921 gatgaggaac gccgcctggg cgcggtcctt gaccgctttc agactgtcca ccccctcacg
     7981 gcggcgatcg gccacgatca gcttgtgaat ggcaaagcgt tccggtcgag gtatgtggat
     8041 cagcacgccg ctgcgatagg tgagcgctgc ggccacgggt tcggcaatca ggtagttcag
     8101 gtaatgcagt gcctgtgctt ccacccccag tgccgccagc ggacggatgc cctcatccgc
     8161 gtcgaaagac ggcgtcagga attccaccaa caggttgctg cgcgtctgcc gccagcgcca
     8221 taccttgccg ggatcgaggc tgggcacggg atcaaattca aaatcggcca gaacgttctg
     8281 caacgactcc gacaccacat cctcaagcgc cagcgacaag cgctcgaaac tcgcgatgtc
     8341 gatgtcattg gtctgggccg tgtcgtccaa ccgatagcga aggccaagtt cgccctggta
     8401 gaggcgaaat gccgccgtcc ccaccagcac gccgccaagc cggaatacgc cggccttctg
     8461 catggcgcca agcaacgatc cggtggcggc gtcggttccc ataaaacctt ccgcccgcag
     8521 gacgcggatc agccgcgcgc gatagcgctg gcgctcgaga tcgtccgcac cggggcgctc
     8581 acgctccagt cgcgcgcgca attccggcgt atcctcacca atgtagctgc gccggaccga
     8641 ggccccgata cgatacatgt cataccaata cgcgcgtttc ccacgcgtca cccgtgaagg
     8701 cgtgcctcgc acggccgcaa cggcctcatc ctgcagggcc gcgacgagat cgacataggt
     8761 ggtttgagcc agaggggaga gccgttcgat catgctcgtg ttatacaaac ttttattttg
     8821 tagagcaata ttatgtgcaa caattcttat tttcgtagag cacgtccctt accgcactcc
     8881 cgcctctccg tccggtgcag cactcagcgc tgctgccgca aaacggctcg gcgcctcgac
     8941 tgacagatcg gtgcataagc atcaaacaga gcctgatcca caaatatttc tatatgtatt
     9001 ttctcatata ctgagattta atattgtgta aatatatctt gcattatgtt aatcctaaaa
     9061 taataataac tttgcagatc aaggagaggc attatgcacc atacgcacgc aattgttatt
     9121 ggcctatttg tgaccactct ttcggtaact gctcatgccg cttgtcaaac aagggggggg
     9181 ggaatttttt cccagatcag aatgattcag tgacttacta ttctacctcg gacaatggcc
     9241 aatgcacgca tggctaccgc gcaggcggcg gaacgatgtt cacatccgca agcatcgtag
     9301 gcaggccgca aaatggaacc ttgcaacaat ccggcgcctt caatttcacc tatcgcgcga
     9361 agcctggctt caagggggct gatagttacg tcatcaaggt ttgcgggaac agccgggggc
     9421 gcgacggctg ctcgacgctg acatatcaga tgaccgtcca ataaagcccg ggccgttgtg
     9481 tagtgctccc tcccgcgaag ggcgggggca gccctcaccg cgccatgtcg aacagcagga
     9541 tttccgcgcc cttgctgcgt ccttcgatgg tgatggcatc catgtcggcc atggcaacgg
     9601 cgtcgccctg cttcagttcg gagccattca cggcgaccga ccccagcgcg acctgaagcc
     9661 agccgatgcg gccggggcgg aaggtgtgcg ccagcttctc accctcgttg aggatgctcg
     9721 cataaaggtc gacatcctga ttgatgtgca cagacccatc gcgggccccg cgtgaggcga
     9781 tgagttgcag cctgccgcgc ctgccggcgt cgccaaggtg cttttcctcg taggacgggg
     9841 cgaggccgcg cacttcggga agaatccaga tttgcaggaa atgcgcttca tcggtcgccg
     9901 aggcattgaa ctccgaatgg cggatgcctg tgccggcggt catgcgctgc acatcgccgc
     9961 ggcggatggt tgagccggaa ccgaggctgt ccttgtgggc gaggctgccg gacagcatgt
    10021 aggtgacgat ttccatgttg tcgtgcggat gcatgggaaa gccgccgccg ccggcgaccc
    10081 tgtcatcatt gatgacccgc aacgggccaa agcccatgaa ctgggggtca taatactggc
    10141 cgaaggaaaa gctgtggtag ctgtcgagcc agccgaaatt cgcatgtccc ctctccgccg
    10201 atttgcgcac atggatcatc gtgtctctcc tgctaagatg gtctacagat cgggaggaat
    10261 cggtagggtt caagcgcttg agaccgcaga atagactttg caatgtcgca atatacatca
    10321 ctcgtggcag gcaaccgcga tgatcattcc gcttatcatc gctgcggtgg acacccggcc
    10381 cgaatggggg cagatcctgc tgagctgctg ccccggccgc gccgatcagg atcacgatct
    10441 cgaccatgac atcggcgtgc tcaagcgcca caaggtggcc gccgtcatca cgctcaatgc
    10501 actcgaagag cttgacgaaa tgggggtcgg ctatctcggt cgcgccgttc atggagccgg
    10561 catggagtgg ttctacctgc cgatcgagga ttactgtcgc cccgatgccg attgggagcg
    10621 cgactggctg atgaccggtc cgcagatccg ggggatgatc cgcgacggca agaacattca
    10681 tatccattgc cgcgccggtt gcgggcggtc cggcacattg gtggcacgcc tgctggtcga
    10741 actcggcatc tgcgaccctg aggaggcgat acgccgcacc cgctttgccc gcccttgcgc
    10801 catcgagcgt cccgagcagg aggatgtggt gcgcgaggca aggccgatcc attgatctga
    10861 tggaaccaga ccgactgctc tatccctttg ttttggcgta tcaccttatc tatcaacgga
    10921 tatccacctg atgggatgat gctcttgcca gcattatgtc gtggatggcg gctcggtttg
    10981 acggtcggtc gaccttccgc ccatggcgaa ggccacaaag cggcgctatg aaaccggtga
    11041 tgcgcaccag ccctgccccc ggccctgtcg atgatcttgc caaccggctg cgcgccggcg
    11101 atcgcgccgc gctggcgcgg gcgatcacgc tggttgaatc caagcgcctc gatcatcagg
    11161 cccaggcgcg ggcgctggtg caacgattgc tgccgttcac cggcaaggct gtccggctcg
    11221 gcatcaccgg tgtgcccggc gtcggcaaat cgaccacgat cgacgtgctg ggcatgcgat
    11281 tgatcagcga ggggcacaag gttgcggtgc tggcggttga cccgacctcg tcgcgcaccg
    11341 gtggcgccat cctcggcgac aagacccgca tggcgcggct gtcgatgcat gaaagcgcct
    11401 ttattcgccc ttcgccggcc gggcagacgc tgggcggggt cgcggcgcgc acacgcgagg
    11461 cgatgctgct gtgcgaagcg gccggatttg atgtcgtact ggtggaaacc gtcgggatcg
    11521 gccagtcgga gacggccgtg gccgacatga ccgatttctt cctcgtcctg ctgctgccgg
    11581 gcggcggcga cgaattgcag ggcatcaaga aaggcgtgat tgaactcgcc gacatgatcg
    11641 ccatcaacaa ggccgatggc gagagcctcg ggcgggcgcg ctccacggcg atggaatatt
    11701 cggccgcgct taacctgctg acccccgaca gcgcgcgctg gaagccgccg gtgatcctga
    11761 tttccggcct caacgacgaa ggattggacg ggctgtggtc gaacgtatcg cagcatcgcg
    11821 ccctgctcgg cgcatccggc gatcttgccg caaaacgtgc ccggcagcag gtcaaatgga
    11881 tgtggacgat gctcgacgac cggttccgca gccgcctgcg cgccgatgcc acattgcggg
    11941 cgcggctgcc ggagatcgag gaggccgtgt tcaccggcgc gctgtcggcc acgctcggcg
    12001 ccgatgaaat cgccgcgatg gtggggctgt gagcaaccgc gtgctgctcg tcaccggctt
    12061 cggcccattt cccggcgtgc cacgcaatcc tgccgaacga accgcccgcg ccatcgccgc
    12121 cgatggtggc ttgcgccgca tcggtctcac aacccattgt gcgatcttcc acaccagtta
    12181 tgccgcgctt gagggcgagg ccgggcctct gctcgcgcaa gtgcagcctg ccgccatcct
    12241 gatgatcggg gttgccagcc ggcggcggca cctgtcggtg gaactcaatg cccgcaaccg
    12301 ccgcgccctg ctgtttcccg atgctgccgg ccagcgctcg gcacggctgg cgatcaaccc
    12361 gagcatggcc tttctcagca agggccgcgc gccgtttgca gcctgcctgc gcgccggaca
    12421 cgccgccgga ctcgacacgc gcctgtcgac aagcgccggc acctatctgt gcaattacag
    12481 ctactggctc atgctggagg caaccgacgc gcgcatcccg tgcctgttcc tgcatattcc
    12541 gaaactgcgc gggccgtggc atttccagcg actggtcagg gccggcgtcg ccatggcccg
    12601 cctgcttgtt caggatgcgc gctggcgctc aaaccgataa cgcgcgcaca gctgcgctcg
    12661 gacgtacgcg ccccacctcg atgccgttcc agttaaccag cggcgtatcc atggcggcgg
    12721 ccaccacggt gccttgcgcg gcatcaagcg gcagacaggc ggcgatgagg ctcgccatca
    12781 ccgccgctga ggcgcgcttg gcgccatcat cgatcttgag tgcaatgccg agcccctgct
    12841 ccggcagcgc ggcgcaatag acgccttccg cgccggtctt gacgaaggcc cgctgcccga
    12901 gtgcccgcat gatcgtcgtg tcaaaccggt tgctgccggc caccatgaac ggcgccgccg
    12961 caacggcctg gcggatgcgc gccgccgctc tcgcccgctc gcgaccatac cccatgccgg
    13021 tgccaaagcg ggcaaagccg cgcgccagcg ctgccaacgg cacggcatag gttgggatcg
    13081 aacagccatc gacgccgcgc ggggcatcag acagatcaag cccgaggctg gtctcaagcg
    13141 ccgcggtgac agcgcgctgc aacgggtgat ccggccggac atagcccttc ggatcctcgc
    13201 ccatggtgac agcggcacag atgaatccgg cgtgcttgcc ggaacaattg ttgtgcaacg
    13261 cactcggctt ccggccggcg gcagccaggg cgcgcgccgc ttcctcgcgc atcggccagt
    13321 gagcaccaca ttcgaggcag gttgccgtct gccccgcctt cttcagcatg ctgagcgcct
    13381 gggtagcatg tacctcctcg cccgaatgcg aggcgcaggc gagcgcgata tcggcatcat
    13441 caaggccgaa gcggtctgcc gcacccgatt ccatcagcac cagcgcctgc aaggctttga
    13501 ccgccgagcg cgggaaggcg cgccgctccc catcgcccag cgcagcaacg atccgaccat
    13561 ccgcatccgc gacgaccagc gtgccgcgat gggctgattc gacgataccg ccgcgtgtca
    13621 cctcgacaag aaccggttcg ctcatggcgc cgcccttccc tttttgttgc tccgggccta
    13681 acttgtcgga caaccgccgg tcaatcccgt atcttgcccg tttcccggag tcttccatgc
    13741 attttgccca cacaatggtg cgtgtcaccg atcttgatgc gtcgctgaag ttctattgcg
    13801 gcctgctggg cctggtcgaa acgcgccgca cggagagcga gaaagggcga ttcacgctga
    13861 ttttcctcgc cgcgcccgaa caggccgcac ttgcccgcga caatcgctgg ccggaagtcg
    13921 aactgaccta caactgggat ccggatccgc agccctatgg aatcggtcgc gccttcggcc
    13981 atctcgcctt ccgggtcgat gacatctacg ccatctgcga aaagctgcag aaggccggcg
    14041 tcgccatcaa ccgtccaccg cgcgatggcc gcatggcttt tgtccgttcg cccgacaaca
    14101 tttccatcga actgatccag aagggcgatg cactgcccct cagggagccg tggacctcga
    14161 tggcgaatga cggctggtgg tgagagtttt tacgctcaca gaaaggacgt ccccaccggc
    14221 ccccgaggca cgcggaggtg gccgagcacg ctttgcgaaa tatcggcaaa gctttgcctg
    14281 aggccgatgc agcgcggcgg catgtccgga ccgaaggcaa gcaccggcac cgcctcgcgg
    14341 gtatggtcgg tgccacccca ggtggggtca ttgccgtgat cggccgtgat gatgcagaca
    14401 tcgccaggcc gcaggacggc gtggatatcg ggcagacgcg catcgaactg ttcaagcaga
    14461 tgcgcataga gtggaacgtc gcggcggtgg ccagcctcgg aatcgaaatc gacgagattg
    14521 gcgaagacca atccgccctc cttcagtata ctgaattcgc gtagcgcaag atcgagattg
    14581 gccatgttgc ctggcccctt gacctcgcgg cccgtcgcct tgtgcgaaaa gatatcacca
    14641 atttttccga ccgaaacgac cggccggccg gccgcctcca gcctatccag caaggttggt
    14701 tgtggcgggg cgagcgccca gtccttgcgg ttcggcgtgc gggcaaaatt gccggaggct
    14761 gtgccaagga acggccttgc tatgacacga ccaatgttga gcggccgggt caggcgccgc
    14821 atgaccccgc agattgcata gagccggtcg aggccgaaat gctgctcatg ggcggcgatc
    14881 tgcaacaccg aatccaccga ggtgtaacag atcggccagc cggtccttat gtgttcctcg
    14941 ccgagacgtt cgatgatctc ggtgcccgag gcgtggcaat cgccaaggat accgccgaga
    15001 ccggtctctt tcagaaaggc ggcgatcaga tcggcgggaa aggtcggcac cgagtgcgga
    15061 aaatagcccc agtcgaaggt gaccggcacg ccggcgattt cccagtggcc ggacggcgta
    15121 tccttgccgc gcgatgtctc gatcgcggcg ccagcggcgc cggtgcgatg cgggccttgc
    15181 agtcccgcag gaacccggcc agtggcgatc cggcacgcct cacccagccc gagagccacc
    15241 aaattcggca cacccagcgg gccggcgcgc aggccgtcgc gatcgccttt ggcggcggcg
    15301 caggcttcgg cgatgtggcc cagcgtgtct gccccggcat caccataggc ggcggcgtcg
    15361 ggtgcgccgc caacgccgac cgaatcgcac acaatcagca ggacccgcgg catcggtcac
    15421 tccccgtcag cggcggtgat gcgctcgaca agcagtgccg ggcgttcagt atgctgaccg
    15481 atatggtagg cggcacgaac cgccgccacc gccgcttcgg cagccgcctc atcgcgcgca
    15541 tgcacgatcg ccagcggcgt atcggctgca acgatcgggt ccagggccac cagatcggtc
    15601 agccccaccc ccgggtcgat ccgatccgcc gcgcgagtgc gaccgccccc cagcatgacc
    15661 acggcaaggc cgagcgcgcg ggtgtcgacc gccatgacac gtcccgagcg ccccgcagga
    15721 acaggccgca tcacgggcgc gcgcggcagg tagcgctcag catgctgaac gaaatcgacc
    15781 ggcccgccga gtgctgccac catgcgcccg aaggtttccg ccgcggcgcc gctgtcgagc
    15841 gctcccgcga tacgggcacg ggcatcgtca agatcgtccg ccagcgcgcc gaccagcagc
    15901 atctcggcgc ccagcgcggt cgtgacctcg atcaggcgcg cgtcccgccg cgcaccggtg
    15961 agattatcca ctgcatttct gacttccacg gcattgcccg cagcgggtgc cagcgcttcg
    16021 ttcatgtcgg tgatcagcgc atgggttttc agcccggcgc cgttggccac agcgacgatg
    16081 ctgcgagcca gcgcacgggc cttttccagc gtcggcatga aagcgcctga accggtcttg
    16141 acgtccatcg caaggccctg cagccccgcc gccagcttct tcgacaggat cgaggcggtg
    16201 atcagcggca ccgattcgac cgtcgccgtg acatcgcgga tggcgtagag cttcttgtcg
    16261 gccggtgcga ggcgctcggt ctggccgata atggcgcatc ccaccgtgcg gacaacggcg
    16321 cggaagcggc cgctatcggg ctggctgaca taaccgggaa tggaatcgag cttgtcgagt
    16381 gtcccgccgg tatggccgag gccacgcccg gagatcatcg gcacaaaacc accacaggcg
    16441 gccaccgccg gggcgagcag cagcgagacg ttgtcgccta cgccgccggt cgaatgcttg
    16501 tcgagcaccg ggccgggcag tgattcacgg ctccagtcca gcacctcgcc ggaatcgcgc
    16561 atggcgcagg tcagggccac ggcttcgtca cggctcatgc cgttgaagaa caccgccatg
    16621 gcaaaggcgc tggcctgcat gtcggtgacc cgaccatcga ccagaccggc gatgaaatcg
    16681 ctgatttcgg tggcggtcag cgttccaccg tcgcgcttcg tgcggatgat ttcctggggc
    16741 agcatggaca tcgagaccgt tagatcacgc tgtgttttgg tggaatcacc aaaacacgga
    16801 caacgtgatc gattctaata gtttagagca tgtcttctgc gattaaccgg catccactta
    16861 atcgcgacat gctctagcgg ctcgtcaata gcgcaactgt gccgtcggcg gcgcgtcact
    16921 tttgatgaca gcggcgagac tggcgtgaag cccggaggcg ccgaagcgga atgtctctgg
    16981 acgggcccag ccggggccca tcacgctatc ggcaaggtca agatagagcc gcgcgtctgc
    17041 gagcgtacgg atgccacccg agggtttcaa cccgacaggg cggccggccg cccttatggc
    17101 ttcaagcatg acgcgcgccg cctcgggcgt ggcgccggtg gcgatcttgc cggtcgaggt
    17161 cttgatgaag tcggcgccgg cggcaatcgt cagatcggcg gcgcggcgga tgctcgcggc
    17221 atccggaaag gcgccggttt ccagaataac cttgagccgc ttgccgaacg gaatatcccc
    17281 gcgcaccagc cgcagcatca gatcgcatac gcctgccccg ccggccagga acatgcgata
    17341 gggcagaaca aaatcgatct catccgcgcc gtccgacatc gccatgcggg tttcgtgctg
    17401 cacatcggcg agcgcatggc cgccggtggg gaagttgacc actgtcgcca ccctgatgcc
    17461 ggccagctcc tgccgcgcca gccgcacatg ccgcggccag acgcataccg cagcgaccgc
    17521 cggaatgccg ttgccgccgc gcgccgtcgc acagagatga cgcaccgccg cctcgtcggc
    17581 gccatcgcca agttcggtca gatccatgag ggccatggcc cgccgtgcca gcgaaagatc
    17641 agcctgcatg ctcataggcc atcctccagc aacccgacga tcagccccgc catacgctcg
    17701 gcgccgcgcg ccgccacggt cttggtgtcg tgatgcgaaa tgtccgcgcc agcaaagccg
    17761 gcggcaaggt tggtcaccat tgacaatgcc gccaccctga ggcccaggcg ccgggcgatg
    17821 atcacttccg gcaccgtcga catgccgacc agatcggcgc caagcgcctt catcgcgcgg
    17881 atctccgccg gggtctcgaa ggtgggaccg gagaaccaga agtagacacc ttcgcgatag
    17941 ggcagcccct gccgctccgc caccgcccgc aagcgggccc gcaagcccgg atcgtagcaa
    18001 tcgcgcatgt cgacgaagcg cgcatcgccc gcctcgccga tcagcggatt gcgcccggac
    18061 cagttgacat agtcggaatg gacgaccacc gaggtcggcc ctacctcggc acgcagcgag
    18121 ccggcggcat tggtgagcag aagtgttttc gcccccagcc cgaccagagt ttccagcgcc
    18181 gggcgcatgg cggcggcatt gccgtgttcg taatagtgtt cacggccgca aaacaccgcg
    18241 acgcggcgcc cgccgaggcg accgatcacc agtcgagcag cgtggccgga gacgccgccg
    18301 ccgggaaagc cttcgagatc gccataggca atggcgaccg cctcctccac ccgatcggcc
    18361 agcgggccga ggccggagcc tagcaccagt ccggcctcga ccggctgcct gccggcgcgt
    18421 ccgatgatgg tctggagcgc actcatccga ggttctccgg cccgaaggca tgcggcagca
    18481 gttcatccag cgtgaagctg cggcgcaggc cctccggccc ggcgatatga atggggcaat
    18541 tgggcctggc gaattcgcgg atgcgctggc ggcaggcgcc gcagggcgtg cagagctggc
    18601 catcc
//
LOCUS       DAOMXG010000031        18436 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_1738_length_18436_cov_10.484196, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000031 DAOMXG010000000
VERSION     DAOMXG010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 18436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18436
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1738_length_18436_cov_10.484196"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1302)
                     /gene="argJ"
                     /locus_tag="PLQ11_03660"
     CDS             complement(<1..1302)
                     /gene="argJ"
                     /locus_tag="PLQ11_03660"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HRK24032.1"
                     /translation="MAGKSVPVSPLAPKKFPRAPQLEGVVFATAEAGIRYRNRQDVLL
                     VTMPKGTSVAGVFTKSKCPSAPVDWCRANLAGGSARALIVNSGNANAFTGARGRQAVD
                     LTADIAARELGVARGEIFLASTGVIGEPLDATKFSGVIGDCARRAEGANWLDAARAIM
                     TTDTFPKIATRKAKIDGHEVVINGMAKGAGMIAPDMATMLSFVFTDAPIAQGPLQALL
                     ARGVKNSFNAVTVDGDTSTSDTLMLFATGTAAKNGCPRITEVGDARLGGFKRALNALL
                     LELAQYVARDGEGARKFVAVNVTGATSARSARKIGFSIANSPLVKTAIAGEDANWGRV
                     VAAVGKAGEPAERDRLTISFGPYRVAHAGARDPDYSEEAVSAYMKNDRIDITVDIGLG
                     RGKDTVYTCDLTKAYVEINGDYRSRSSAVNQAIACCRSLPHI"
     gene            complement(1325..2176)
                     /locus_tag="PLQ11_03665"
     CDS             complement(1325..2176)
                     /locus_tag="PLQ11_03665"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRK24033.1"
                     /translation="MSPLLKYPLAAFILTFGATVALAQGAKPPASIDGVPITEQDLKL
                     AQDDIGGSMQGMNDDQKKRYLVDYMLDLKLLARAAEKEKLAETPDFARKLAYLRDRAL
                     MEELMAREGASATSDDKLKKFYEEAVKGLPPEEEAKARHILVPTEDEARKVADRLKAG
                     EDFGKVSTEVSKDPGSAKEGGDLGWFTKDRMVKEFADAAFALKAGETSAPVKSQFGWH
                     VIRLDEKRIRPVPPLSEVKEELSRYLAQKAQQDMILKLRATARIERADPVKSDADKAA
                     PAEPKKP"
     gene            2527..5319
                     /gene="secA"
                     /locus_tag="PLQ11_03670"
     CDS             2527..5319
                     /gene="secA"
                     /locus_tag="PLQ11_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HRK24034.1"
                     /translation="MFGAILKKIFGSSNDRRLKRYRPVVAAVNALEPELEALTDEALA
                     ARTAEFKRQVAAGEKTLDDLIVPAFATVREAAKRTLKQRHFDVQLIGGLVLHEGAIAE
                     MRTGEGKTLVATLAVYLNALSGKGVHVVTVNDYLARRDSEWMGRIYRFLGMSVGVIVH
                     GLDDAERHAAYACDITYGTNNEYGFDYLRDNMKYDMAQMVQRGHNFAIVDEVDSILVD
                     EARTPLIISGPLDDRSELYAAIDAIIPRLTKEHYELDEKQRTVNLTEAGNETMEQLLL
                     EAGLLKDGTLYDAGNATLVHHVNQALRAHRLFTRDKDYIVHNGEVVIIDEFTGRMMPG
                     RRYSEGLHQALEAKERVQVQPENQTLASITFQNYFRLYEKLAGMTGTAATEAEEFQQI
                     YNLEVVEIPTNVPVKRKDDDDEVYRTAAEKYAAIVKLIEECQQRGQPVLVGTTSIEKS
                     EELAHYLTKDGFRQIDLGDANAFAPLYRAAHGGKAEKIFAVLNARFHEQEAYIVAQAG
                     VPGAVTIATNMAGRGTDIQLGGNLDMRIKQEITGLDEGSADYAARAEAIRQEVAILKG
                     KALAAGGLYVIGTERHESRRIDNQLRGRSGRQGDPGHSRFFLSLEDDLMRIFGSDRMD
                     SMLQKLGLQPGEAIAHPWVNKALETAQKKVEARNFDIRKNILKYDDVMNDQRKVVFEQ
                     RREMMAQEDMADQIREMRHGVIDDMIARGIPEDAYPEQWDVAELKKGVATYLNLDLPV
                     DEWAKEEGIADQEIRERLFKAADESYAERVARNSPEAMRYIEKQVVLQTLDHLWREHL
                     VTLDHLRQVIGWRGYAQRDPLNEYKSEAFDLFDGLVTRLREHVTAQMMRVEVQFEELP
                     ADPQLPPMQGHHINPQTGEDEMAGEAPAMLPQSTKARTEEIDPARPETWGKVGRNEPC
                     PCGSGKKFKHCHGAYA"
     gene            complement(5329..6069)
                     /locus_tag="PLQ11_03675"
     CDS             complement(5329..6069)
                     /locus_tag="PLQ11_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRK24035.1"
                     /translation="MALDVIDLRQFYSSRLGAIALGSILGAVRARWSNPAGLSILGLG
                     YPLPYLDAFREQAERCIALMPGRQGVTAWPTGMLSSVGLVDPLDLPLRESVIDRLLVI
                     HALEVSEDAHALMDECWRILAPGGHLIVVAPNRSGSWARRDSTPFGHGQPYSRGQLTQ
                     LMRQSLFTPIHWTEALHIPPLRGSLSLSFAPAIERFGNTLNLPFAGVHIIEASKQLYR
                     PVPARRAARIGARLDPVLVPSVGRNAGN"
     gene            6128..6928
                     /gene="gloB"
                     /locus_tag="PLQ11_03680"
     CDS             6128..6928
                     /gene="gloB"
                     /locus_tag="PLQ11_03680"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HRK24036.1"
                     /translation="MVAGVCPDTEECGVQIVQFPCLSDNFGVLIHDPKSGATAAIDVP
                     EAGAVRAALAAEGWRLSHILVTHRHHDHIAGIAEIVAETGAKVIAPAGAADVVPGAER
                     YVREGDRVDVGGLSAEVWDAPGHSPDHVIYHFASDKVAFVGDVLFALGCGRVFDNDYD
                     RMWQALARLGGLPDATALYFGHEYTVSNGRFALSVDPGNAVLQAAVAEAEAVRARGGW
                     TSPTTVGRERAANPFLRATDPAMAARLGLAGQPAAAVFRELRERKNRF"
     gene            6930..7685
                     /locus_tag="PLQ11_03685"
     CDS             6930..7685
                     /locus_tag="PLQ11_03685"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRK24037.1"
                     /translation="MVFSGDLALVAACERRIVNAWPAIDKLMMDGWVVRLAGGYSARA
                     NSASPMLPGARLDAPLLAVIEATYRAAGLQPTVRVTPLADQSVDAVLAARGYRQRDAS
                     WGMIAPLTPGPVTTGGVRLEGSASTEWVRGVSALQEGSKRNADAALMEIVGRVQLPAA
                     FATLVEGGRPVGFGMSVVESGMAEIGSLVIDPAVRGRGLGRILVQTLMGWAHAEGAQQ
                     AYLQVEETNRGALALYRSLGFAELYRYRTMVAG"
     gene            7914..8825
                     /locus_tag="PLQ11_03690"
     CDS             7914..8825
                     /locus_tag="PLQ11_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRK24038.1"
                     /translation="MPQNVPRPLAFFALVVGAVAMGCSPVFVRLADVGPFASAFWRVA
                     LALPLLWLWHRHAAEPRPAESWRFAVAVIASGIAFAGDLIFWHLAILNTTIANATLLA
                     TLAPVWVFLVAFLVLGHALRRAEVIGLAACLIGGSLLVRDSFDYAPERLWGDAAGFAT
                     SLFFGLYIVFITHSRRFSGPARVMLLSTAVTAIVLLCAALLAGERFLPGSPAGWLTLV
                     LLALISQVAGQGLLAYALGQLPVAFSSLVVFLEAIAAALLGWLAFSEHLGAWQWIGGG
                     FVLFGIWIARPVLQRAATDDSGRVGLQ"
     gene            8822..10114
                     /locus_tag="PLQ11_03695"
     CDS             8822..10114
                     /locus_tag="PLQ11_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HRK24039.1"
                     /translation="MNRSQAHDTARRIFDAAVQRAQPRSYVARYLPKPKERGRLLLLG
                     AGKAGASMVQAAEDHYLNQLGVDRARILGLGITRHGYACKTSLVPIIQAGHPVPDLFG
                     LAGTERMLTLADRAGPDDTAVVLMSGGASANLVAPAGALSLTDKQVITAALLRSGAPI
                     DAINCVRKHLSRVKGGRLAARLAPAEVYTLAISDVPGDDLGVIGSGPTVPDETTQAEA
                     LAILERYRIAISPAVAAVLTDPALACPGKDHPAFQRTRSEIIFRPCEALAAAREVAAA
                     AGYEVIDLGADVAGEARTVAAAHAAMAVEAHATGRRVAILSGGELTVTIRGKGRGGPN
                     QEYAMALAMALDGLPGMVALAGDTDGTDGGGGQATDPAGAIVDGQTLAAARARGLDAG
                     ALLADNDSTRFFTHAGGLITTGPTLTNANDLRVILVDP"
     gene            10143..10547
                     /locus_tag="PLQ11_03700"
     CDS             10143..10547
                     /locus_tag="PLQ11_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1036 domain-containing protein"
                     /protein_id="HRK24040.1"
                     /translation="MAGALALPAGPVTADLRICNTTAHRTGVAIGFQDQTTWVTEGWF
                     NLRPGACETILKGKLNSRYYYIFGQDYDRGGEWSGPNYLCIRDQEFSIRGPQDCYARG
                     YERAGFLEVDTGEQADWTIELGDGGQVGGRAQ"
     gene            10581..12005
                     /gene="pyk"
                     /locus_tag="PLQ11_03705"
     CDS             10581..12005
                     /gene="pyk"
                     /locus_tag="PLQ11_03705"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRK24041.1"
                     /translation="MIRNRRTKLIATLGPASMEDATVAALANAGADVFRINMSHSSHE
                     VLRQRVAQIRTIEKWVGRPIGILADLQGPKLRVGRFAAGPAELKNGARFRLDADATPG
                     DASRVQLPHPEILQSVVAGHRLLIDDGKLCLRVTETGPGFFETVVEVGGPISDRKGVS
                     LPDTMLPVGALTDKDRADLDAALSANVDWIALSFVQRPEDVAEVRKVARGRALVMAKI
                     EKPQAIERLDEIMEIADALMVARGDLGVEMPLEVVPGLQKRITRVARRYGKPVVVATQ
                     MLESMITSPAPTRAEVSDVATAVFEGADAVMLSAESASGKFPLEAVSTMSRIAEQVER
                     DSNYKIIIDGQRADPEPTGADAIAAAARTVAETLDLKAIVAWTASGATALRIARERPY
                     TPIIMLTTSERTARRMQLVWGINPVIGADPTHTADMVRIGTTAARNSGLAQLGQRIIM
                     VAGVPFGTPGSTNMLRIAFVSSDS"
     gene            complement(12056..12655)
                     /locus_tag="PLQ11_03710"
     CDS             complement(12056..12655)
                     /locus_tag="PLQ11_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRK24042.1"
                     /translation="MARLFFAIILTVALQGIASAQQGRPAPSSDLPPGAKVLPPPVTL
                     DALYKQLRTARDPNEARPIAKQIEQRWMRSGSDTTNLLMDRVLEVMSAKAYDTGLELL
                     DHILQLQPNWAEAYAKRATLLFLMDDYDGAMRDLRAVLAREPRHFSALAGLGMIWQRL
                     GKPKAAYAAYQKVLEVYPHLVEVREIVDRMRPEVEGREL"
     gene            complement(12732..12857)
                     /gene="ykgO"
                     /locus_tag="PLQ11_03715"
     CDS             complement(12732..12857)
                     /gene="ykgO"
                     /locus_tag="PLQ11_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012177066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HRK24043.1"
                     /translation="MKIRNSLKSLMKRHRDNQLVRRRGRVYIINKTQKRFKARQG"
     gene            13202..13954
                     /locus_tag="PLQ11_03720"
     CDS             13202..13954
                     /locus_tag="PLQ11_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018260787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRK24044.1"
                     /translation="MFDSTLLVGKSALVTGASSGLGRHFSAVLARAGAEVTLAARREV
                     MLAEAAGAIRQAGGRVVTARLDVTDPQTVEEVCGQRAFDIVINNAGVTHDGKALTVSI
                     EDWRHVLETDLTGVFRVAQAAANRMVAENRPGSIVNIASILGHRVAGNLAAYAAAKAG
                     VIQLTRSLALEWARHRIRVNALCPGYIATDLNRAFFASSAGQALIGRIPQRRLGQLSD
                     LDGPLLLLASDAGAFMTGTDIVVDGGHLVASL"
     gene            13964..15169
                     /locus_tag="PLQ11_03725"
     CDS             13964..15169
                     /locus_tag="PLQ11_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018259567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRK24045.1"
                     /translation="MDFTISPRIEDFRSRIARFVEDKILPLEADRSSYDAHDNIRLDL
                     LEALRQRARTEGLWCLQLKPETGGQGLGKVGMAVCYEAMNRSIFGPVSFNSGAPDDGN
                     MMVLEQVATPAQQERWLAPIVAGKVRSAFAMTEPHPGGGSDPSMMLTTATRRGDRYVI
                     RGRKWFITGAGEAQHFILLARTSEDPRRGLSAFLFDRDTPGWRIDRRIPIMGPEEHGG
                     HCELVFDGLEIPVSDLLMNEGDGLKLTQIRLGPARLTHCMRWLGLARRCVDIARTYAA
                     ERFAFGERLAKRESVRMMLGDLAMQIEIGRLLVMKAAWELDRGGYARKEISMAKVHVA
                     NLLHKAADTALQINGARGYSKDTPLEWIYRYARQARLVDGADEVHKMVLERNLASEGQ
                     DFWRWPVGQ"
     gene            15188..16207
                     /locus_tag="PLQ11_03730"
     CDS             15188..16207
                     /locus_tag="PLQ11_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019170348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HRK24046.1"
                     /translation="MNAATDFDDTPLRAFLMSRFGETPSFRLERIAGGQSNPTYFVDH
                     GEHRFVLRKQPDGPLLRGAHAIDREYRVLTALNPTGVPVPRPILFHEAPDIVGTPFYL
                     MERVEGRIFASGALPEAAPGERNALWMGLAKALAHLHALRPDEVGLGDFGRPGNYFER
                     QLARWSRQWHESSSQPIPELDALAAWLADHLPADDGAVSIAHGDYRMGNVIFHPTEPR
                     VVAILDWELATLGHPLADVGFCAMAWHTAPEEYGGILGLDLAACGLPTQEQFVSCYAR
                     ANPSVAELQPFHLAFALFRFAVIFVGIADRARQGTAAGTDARSIGPLARRFAVRALEV
                     ISGKH"
     gene            complement(16709..16783)
                     /locus_tag="PLQ11_03735"
     tRNA            complement(16709..16783)
                     /locus_tag="PLQ11_03735"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16749..16751),aa:Val,seq:gac)
     gene            complement(16896..17258)
                     /locus_tag="PLQ11_03740"
     CDS             complement(16896..17258)
                     /locus_tag="PLQ11_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRK24047.1"
                     /translation="MTKILLAEDDNDMRRFLVRALQNAGYDVAAFDNGKSAYHRLREE
                     PFELLLTDIVMPEMDGIELARRATELDPDIKVMFITGFAAVALNPDSQAPKDAKVLSK
                     PFHLKDLVNEVKKMLAAA"
     gene            17445..18368
                     /locus_tag="PLQ11_03745"
     CDS             17445..18368
                     /locus_tag="PLQ11_03745"
                     /inference="COORDINATES: protein motif:HMM:NF016873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HRK24048.1"
                     /translation="MPAMPAELPPDEAGCLPGCSEFDPPFELVEPVAVSAPVLFNSPH
                     SGVTFPPSFLAQTPLSQADLCRSADLHVDRFLDGPLALGMSVLRVNIPRSFLDLNREA
                     HELDPTMFAGRLPPPVNSGSLRVSSGYGTIPRLVAEGVAIYRRPLPVAEALRRIDCYY
                     RPYHRLLATHLARLRQAFGSAILIDCHSMPSHSGGHALADIVLGNRNGRSCSPELMRL
                     AFHWFESLGLSVACNTPYAGGFITEHYGKPGLGQHALQIEVHRALYMDEKSYVPHEGL
                     ARLADAMTGFAARLVDYFNGQERPAPQWAAE"
BASE COUNT         3248 a         5752 c         5969 g         3467 t
ORIGIN      
        1 aatgtgcggt aacgatcgac agcaggcgat cgcctggttg acggcgctcg aacgcgaacg
       61 gtagtcgccg ttgatctcga cgtaggcctt ggtcaggtcg caggtgtaga ccgtatcctt
      121 gccgcgcccg aggccgatgt cgaccgtgat atcgatgcga tcgttcttca tataggcgga
      181 gaccgcctct tccgaatagt caggatcgcg cgcccctgca tgcgccacgc gatagggccc
      241 gaagctgatg gtcagccggt cacgctcggc cggctcgccg gccttgccga cggcagccac
      301 cacgcgaccc caattggcat cttcgccggc gattgcggtc ttgaccagcg gcgaattggc
      361 gatggagaag ccgattttcc gggcggagcg cgccgaggtc gcgccggtca cgttgaccgc
      421 gacgaatttc cgcgcaccct cgccatcgcg ggcgacatat tgcgcaagtt caagcaggag
      481 ggcgttcagc gcccgcttga agccgccgag ccgcgcatcg ccgacctcgg tgatgcgcgg
      541 gcagccgttc tttgcggcgg tgccggtggc aaacagcatc aaagtgtcgg aggtcgaggt
      601 gtcgccatcg acggtcacgg cgttgaacga gttcttcaca ccacgcgcca gcagcgcctg
      661 caacggacct tgcgcgatcg gggcgtcggt gaaaacgaag ctcagcatcg tcgccatgtc
      721 gggcgcgatc atgccggcgc ccttggccat gccgttgatg acaacctcat ggccgtcaat
      781 cttcgccttg cgggtggcga tctttggaaa cgtgtcggtg gtcatgatgg cgcgcgccgc
      841 gtcgagccag ttggcgcctt ccgcgcggcg ggcgcaatcg ccgatcacgc cgctgaactt
      901 ggtcgcatcc agcggttcgc cgatgacgcc ggtggaggcc aggaagatct ccccccgggc
      961 gacgccgagt tccctggcgg cgatgtcagc ggtcaggtcc acggcctgcc ggcccctggc
     1021 gccggtaaag gcgttggcgt tgccggaatt gacgatcagc gcgcgggccg agccgccggc
     1081 aaggttggcg cggcaccagt cgaccggggc cgaggggcat ttggacttgg tgaaaacacc
     1141 ggcgacgctg gtgcccttcg gcatagtgac cagcagcacg tcctgccggt tcctgtaacg
     1201 gatcccggcc tcggcggtgg caaaaaccac gccttcgagc tgcggtgctc tgggaaattt
     1261 cttcggcgcc agcggcgaga cggggacgga ctttccagcc atgggagtat cctgtcagac
     1321 ggtgtcaggg cttcttgggt tccgccgggg cggccttgtc ggcgtcggac ttgaccggat
     1381 cggcgcgttc gatccgggcc gtggcgcgca acttgaggat catgtcctgc tgcgccttct
     1441 gggcgagata gcgcgacaat tcttccttga cctcggacaa tggcgggacc gggcggatgc
     1501 gcttctcgtc aaggcggatg acatgccagc cgaactggct tttcaccggc gccgaggttt
     1561 cgccggcctt cagcgcaaag gcggcgtcgg caaattcctt gaccatgcga tccttggtga
     1621 accagccgag atcgcctccc tccttggccg agccggggtc tttcgacact tcggtcgaaa
     1681 ccttgccgaa atcctcgccg gccttcagcc ggtccgcgac tttcctggct tcatcctcgg
     1741 tcggcaccag aatatggcgg gccttggcct cctcttcggg cggcaggccc ttgacggctt
     1801 cttcgtagaa cttcttgagc ttgtcgtcgc tggttgcgct ggcgccttca cgtgccatca
     1861 gctcttccat cagggcgcga tcgcgcagat aggcaagctt gcgggcaaaa tccggcgttt
     1921 ccgccagctt ctccttctcg gctgcgcggg cgagcagttt cagatccagc atgtagtcaa
     1981 caagataacg cttcttctgg tcgtcgttca tgccctgcat cgagccgccg atgtcgtcct
     2041 gggcaagctt gaggtcctgc tcggtgatgg gaacgccatc gatgctggct ggcggcttgg
     2101 ccccctgggc cagcgcgaca gtggcgccaa aggtcaggat gaaggcagcg agtgggtatt
     2161 tcagcagggg ggacatgtcg actccgctca atcggatgat tcctgatact gcctcgggtc
     2221 cggttggcgc aatgctgaat gcgctccttt gcggatttgt cggggcaggg cgctgcgccg
     2281 gcagcgatac cggggcggga tgcggccaat aggcacccgc gctgcccttt gccaagggaa
     2341 atggcggccg cttgattgac ttgacggggg ctaatcccta tgtgcgatgc gcgccccggt
     2401 cgatggccgg cggcgcgttc cctgttgcgc gaatcaccgc ttacggatgc ttctgcgcga
     2461 gcaacccagc cgcccccgtg cggcgagacc ggcaccgggc cactggtgcc agcccaggac
     2521 ttttcgatgt tcggcgccat tctcaagaaa atcttcggtt cctccaacga ccggcggctc
     2581 aagcgctacc ggccggttgt cgcggcggtc aatgcgctgg agccggagct ggaggcgctg
     2641 accgacgagg cgctggcggc gcgcacggcc gaattcaagc ggcaggtggc tgccggcgaa
     2701 aaaacgctcg acgatctgat cgttccggcc ttcgccaccg tgcgcgaggc ggccaagcgc
     2761 acgctcaagc agcggcattt cgatgtgcag ctgatcggcg gtctggtgct gcacgagggc
     2821 gccatcgccg agatgcgcac cggcgaaggc aagacgctgg tggccacgct tgccgtctat
     2881 ctcaacgcgc tctcgggcaa gggcgtgcac gtcgtcaccg tcaacgacta tctcgcccgg
     2941 cgcgactccg aatggatggg ccgcatctac cgctttctcg gcatgagcgt cggcgtcatc
     3001 gtccatggcc tcgacgatgc cgagcgccat gccgcctatg cctgtgacat cacctacggc
     3061 accaacaacg aatacgggtt cgattatctg cgcgacaaca tgaagtatga catggcgcag
     3121 atggtccagc gcgggcacaa ctttgccatc gtcgatgaag tcgactcaat tctggtcgat
     3181 gaggcccgca ctccgctgat catctccggg ccgctcgacg atcgttccga actctatgcg
     3241 gcgatcgatg cgatcatccc gcgtctgacc aaggagcatt acgagcttga tgaaaagcag
     3301 cgcaccgtca atctgaccga agccggcaac gagacgatgg agcagctgct gctggaagcg
     3361 gggctgctca aggacggcac gctttatgac gccggcaatg cgaccctcgt tcaccatgtc
     3421 aaccaggcgc tgcgcgccca ccggttgttt acccgcgaca aggactacat cgtccacaat
     3481 ggcgaggtgg tgatcatcga cgagttcacc ggtcgcatga tgccggggcg gcgctactcg
     3541 gaagggctgc atcaggcgct cgaagccaag gaacgggtgc aggtccagcc cgaaaaccag
     3601 acgctggcct cgatcacctt ccagaactat ttccgccttt atgaaaagct ggccggcatg
     3661 accggaacag cggcgacgga agccgaggaa ttccagcaga tctacaatct cgaagtggtc
     3721 gagattccga ccaacgtccc ggtcaagcgc aaggacgatg acgacgaggt ctatcggacg
     3781 gcggcggaga aatatgccgc gatcgtcaaa ctgatcgagg aatgccagca gcgcggacaa
     3841 ccggtgctgg tcggcaccac ctcgatcgag aagtcggaag aactggcgca ctacctgacc
     3901 aaggacggat tccggcagat cgacctgggc gatgccaatg ccttcgcccc gctctaccgg
     3961 gcggcccatg gtggcaaggc ggagaagatc tttgccgtgc tcaacgcccg cttccatgag
     4021 caggaggcct atatcgtcgc ccaggcaggt gtgcccggag cggtgacgat cgccaccaac
     4081 atggcgggcc gcggcaccga catccagctc ggcggcaatc tcgacatgcg catcaagcag
     4141 gagatcaccg gcctcgacga gggatcggcc gactatgccg ccagggccga ggccattcgt
     4201 caggaagtcg ccattctcaa gggaaaggct ctggcggccg gcggtcttta tgtgatcggc
     4261 accgaacggc acgagagccg gcgtatcgac aaccagctgc gcggccgctc cggccgtcag
     4321 ggtgatcccg gccattcgcg cttcttcctc agccttgagg atgacctgat gcgcatcttc
     4381 ggctccgatc gcatggattc gatgttgcag aagcttggct tgcagccggg cgaggccatc
     4441 gcccatccct gggtcaacaa ggcgttggag accgcccaga aaaaggtcga ggcgcgcaac
     4501 ttcgacatcc gcaagaacat cctcaaatac gacgacgtga tgaacgacca gcgcaaggtg
     4561 gtgttcgagc agcgtcgcga gatgatggct caggaggata tggccgacca gatcagggag
     4621 atgcgccacg gcgtcatcga cgacatgatt gcccgcggca ttccggaaga tgcctatccc
     4681 gagcaatggg atgtggcaga gctgaagaag ggcgtcgcca cctatctcaa tctcgacctg
     4741 ccggtggacg aatgggccaa ggaggaaggt atcgccgatc aggaaatccg cgagcgcctc
     4801 ttcaaggcgg cggacgagtc ctatgccgag cgggtggcgc gcaattcgcc cgaggcgatg
     4861 cgctacatcg agaaacaggt ggttctgcag acgcttgatc atctctggcg cgagcatctg
     4921 gtcacgcttg accatctgcg gcaggtgatc ggctggcgtg gctatgccca gcgcgatccg
     4981 ctcaacgaat acaagtccga agcattcgat ctgttcgatg ggttggtgac acggctgcgc
     5041 gagcacgtca ccgcacagat gatgcgtgtc gaggtgcaat tcgaggaatt gccggccgat
     5101 ccgcagctgc cgccaatgca gggccaccac atcaatccgc agaccgggga ggatgagatg
     5161 gcgggtgagg cgccggcaat gctgccgcaa tcaaccaagg cccgcaccga agagatcgat
     5221 cctgcgcggc cggaaacctg gggcaaggtg ggtcgcaacg aaccctgccc gtgcggatcg
     5281 ggcaagaaat tcaagcattg ccacggcgcc tacgcgtaat ccggtgcttc aatttccagc
     5341 attgcgcccg acgctcggca caaggacagg atccagccgg gcgccgatgc gcgccgcacg
     5401 ccgtgccggc accgggcgat agagctgctt gcttgcctcg atgatgtgaa cgccggcaaa
     5461 gggcaggttg agcgtgttgc cgaagcgctc gatggccgga gcgaagctga ggctgagact
     5521 gccgcgcagc ggcggaatat gcaaagcctc ggtccagtgg atcggcgtga acaatgactg
     5581 acgcatcaac tgggtcagct ggccgcggct gtagggctgg ccgtggccga acggggtcga
     5641 atcgcgccgc gcccagcttc cggaacgatt gggcgcgacg acgatgagat ggccgccggg
     5701 ggcgaggatg cgccagcatt catccatcag cgcatgggca tcctcgctga cctcaagcgc
     5761 atgaatgacg agcagccgat cgatgaccga ttcgcgcaag ggaaggtcga gcggatcgac
     5821 caggccgaca ctcgagagca tgccggtggg ccaggcggtc actccctgcc gccccggcat
     5881 cagcgcgatg cagcgctcgg cctgctcacg aaaggcgtca aggtagggca ggggatagcc
     5941 cagtccgagg atcgacaggc cggcagggtt gctccagcgg gcacggacag cgccgagaat
     6001 cgagccgagc gcgatcgcgc ccaaacgcga gctgtagaac tgccggaggt cgatgacatc
     6061 gagagccatt ggatcagcgt ccaagtgtat gtatcactgg gcgcgccggc aaggcgtcgc
     6121 cgtcggcatg gttgccgggg tgtgtcctga cactgaggag tgtggcgtgc agattgtcca
     6181 gtttccctgc ctttccgata atttcggcgt tttgatccac gatccgaaga gtggcgcaac
     6241 cgctgccatc gacgttcccg aggccggagc ggtgcgtgct gcgcttgcgg ctgaaggctg
     6301 gcggctcagc catatccttg tcacccatcg ccaccacgat catatcgccg gtatcgctga
     6361 gatcgttgcc gagacgggtg cgaaggtcat tgcgccggcc ggtgctgccg atgtcgtgcc
     6421 tggggcggaa cgctacgtgc gcgagggaga ccgggtcgat gtcggcgggc tcagcgccga
     6481 ggtatgggat gcgccgggcc attcgcccga ccatgtgatc tatcactttg cctccgacaa
     6541 ggtggccttt gtcggcgatg tgctgtttgc gctcggctgt ggccgggtgt tcgacaacga
     6601 ttatgaccgg atgtggcagg cgctggctcg gctcggcggg cttcctgacg cgacggccct
     6661 ctatttcggt catgaatata cggtttcgaa cggcaggttt gcgctgtctg ttgatcccgg
     6721 aaatgcggtg ttgcaagcgg ccgtcgccga ggcggaggcg gtgcgggcgc gcggtggctg
     6781 gacctcgcca accacggtcg ggcgcgaacg ggcggccaat ccgttcctca gggcaaccga
     6841 tccggcgatg gcggcgcgcc tggggctggc gggtcaaccg gcggcggcgg tgttccgcga
     6901 gttgcgcgag cgcaagaacc ggttctgaca tggtgttttc cggtgatctg gcgctggtcg
     6961 cggcctgcga gcgccgcatc gtcaatgcct ggccggcgat cgacaaactg atgatggatg
     7021 gctgggtggt gcggctggca ggaggctatt cggcccgtgc caattccgcc tcgccgatgc
     7081 tgccgggagc ccggctcgat gcgccgctgc tggcggttat cgaagcgact taccgggctg
     7141 cggggctgca accgacggtg cgtgtaacgc cgttggcgga tcagagcgtc gatgcagtgc
     7201 tggccgcgcg cggctatcgc cagcgcgatg cctcgtgggg catgattgcc ccgttgacgc
     7261 cggggccggt gacgaccggt ggcgtgcggc tggaagggtc ggcaagcacc gaatgggtgc
     7321 gcggcgtctc ggccttgcag gagggatcca agcgcaatgc cgacgcggcg ctgatggaaa
     7381 tcgtcggccg ggtgcaactg cccgcggctt ttgctacgct ggtcgaaggc gggcgcccgg
     7441 tcgggttcgg catgagcgtg gtcgagagcg gcatggccga gatcggctcg ctggtgatcg
     7501 atccggcggt gcgcgggcgt ggtcttggcc gcattctggt gcagacgctg atgggctggg
     7561 ctcatgccga gggagcgcag caggcctatc ttcaggtcga ggaaaccaat aggggagcgc
     7621 tggcgctcta ccggtcgctc ggctttgccg agctttaccg ataccggacg atggtggcgg
     7681 gctagctaga gcatgtcgcg attacgtgga tgccggtcaa ttgcagaata catgctctaa
     7741 cctattgata ccgatcacgt gatccgcatt ttgatgattc catcaaaatg cagcgtgatc
     7801 tagatgcatt ttccttgtct ttgacccgaa actggtcatc gcaacggttc aatctggtcg
     7861 gacagaaccc tagaaagact tctgaccctg gaagcagcgt tgacggattc cccatgccgc
     7921 agaatgtccc gcgccctctg gctttcttcg ccctcgtcgt gggcgccgtt gcgatgggct
     7981 gttccccggt gttcgtgcgg ctcgccgatg tcgggccgtt cgccagcgca ttctggcgcg
     8041 ttgcgctggc gctgccactt ctctggctct ggcaccgtca cgctgccgag ccacggccgg
     8101 ccgagagctg gcgctttgcg gtcgcagtca tcgcctctgg catcgccttt gccggcgatc
     8161 tgatcttctg gcacctggcg atcctcaaca ccaccattgc taacgcgacc ctgttggcaa
     8221 ccctggcacc ggtctgggtg ttcctcgtcg ctttcctggt gctggggcat gccctgaggc
     8281 gcgcggaggt catcgggctt gcagcctgcc tgatcggagg ctccttgctg gtccgggaca
     8341 gtttcgacta tgcgcccgaa cgattgtggg gggatgcggc cggctttgcc acatcgctgt
     8401 tcttcggcct ttacatcgtg ttcatcaccc actcgcgccg gttttccggc ccggcacggg
     8461 tcatgctgct gtccacggct gtcacggcga tcgtgctgct atgcgcggct ttgctggccg
     8521 gtgaacggtt tctgcccggt tcaccagccg gctggctgac gctggtgctg ctcgccctga
     8581 tctcccaggt cgccgggcag ggattgctgg cctatgcact tggtcaattg ccggttgcgt
     8641 tttcttcact ggtcgtattt cttgaggcaa ttgcagcggc attgctgggc tggctcgcat
     8701 tttccgagca tctgggagcc tggcagtgga tcggcggggg gtttgttttg ttcgggatat
     8761 ggatagcgcg cccggtgctg caacgtgcag cgaccgacga tagcggtcgg gtcggattgc
     8821 aatgaatcgt tcgcaggccc acgataccgc gcgcaggatt tttgatgccg ctgtgcaacg
     8881 cgcacagccc agatcttacg ttgcgcgcta tcttcccaaa cccaaggaac gggggcgcct
     8941 gctgctgctg ggggcgggca aggccggtgc gagcatggtg caggcggccg aggatcatta
     9001 cctgaaccaa cttggcgtgg atcgcgcccg catcctcggc ctgggcatca cccgccatgg
     9061 ctacgcctgc aagaccagtc tggttccgat tatccaggca ggccacccgg ttccggatct
     9121 cttcggcctc gccggcaccg aacggatgtt gacactggcg gatcgggcgg gccccgatga
     9181 tacggcagtg gtgctgatga gtggcggcgc ctcagccaat ctcgtggctc cggctggcgc
     9241 cctgtcgttg accgacaagc aggtcatcac tgcggcgttg ctccgctccg gtgcgccgat
     9301 cgatgcaatc aactgcgttc gcaagcacct ttcacgggtc aagggtggac ggttggcggc
     9361 gcggctggcg ccggccgagg tgtataccct ggcgatatcc gacgtgcccg gcgatgatct
     9421 tggcgtcatt ggctccgggc cgaccgtacc tgacgagacc acccaggccg aggccctggc
     9481 catccttgag cgttatcgaa ttgccatctc gccggcggtc gccgcagtgc tcacagatcc
     9541 ggcgttggcc tgtcccggca aggaccatcc cgctttccag cgcacccggt cggaaatcat
     9601 cttccgcccc tgcgaagcgc tggccgccgc ccgtgaagtc gctgcagccg ccggctacga
     9661 ggtgatcgac cttggggccg atgttgcggg cgaagcgcgc acggttgccg ccgcccatgc
     9721 tgccatggct gtggaggccc atgcgacggg ccgccgcgtc gccatccttt caggcggcga
     9781 acttaccgtc accattcgcg gcaagggccg tggcggcccc aaccaggaat atgccatggc
     9841 tctggcgatg gcactggacg gcttgccggg tatggtcgcg cttgccggcg acaccgatgg
     9901 cacggatggc ggtggcgggc aggctaccga cccggccgga gccatcgtcg atggccaaac
     9961 ccttgcggca gcccgtgcgc gggggctgga cgccggagcg ctgctggccg ataacgattc
    10021 gacccggttt tttacccacg ccgggggatt gatcactacc ggcccgaccc ttaccaatgc
    10081 caacgacctg cgtgtcatcc tcgtggaccc ataatcgccg ggtttggccc cgtgcgatcc
    10141 tgttggctgg cgcgctggcg ctgccggctg gcccggtcac ggcggatctc aggatctgca
    10201 acaccactgc ccaccgcacc ggtgtggcca tcggcttcca ggatcagaca acctgggtga
    10261 ccgaaggctg gttcaatctg cggcctggcg cctgtgaaac catcctgaaa ggcaagctca
    10321 attcgcgcta ttattatatc ttcgggcagg attatgatcg cggcggcgag tggagcggcc
    10381 ccaattacct ctgcattcgc gaccaggagt tttccattcg cggcccgcag gattgttatg
    10441 cacgaggata cgaacgcgcc ggctttctcg aggtcgacac gggggaacag gccgactgga
    10501 ccattgaatt aggcgatggc ggccaggttg gcgggcgtgc ccagtagcgc gcgaacaatt
    10561 cgatcgaaag gaccttcgcg gtgatccgca accgtcgcac caagctcatc gccacgctcg
    10621 gcccggcctc gatggaggat gccaccgttg ctgccctcgc caatgccggg gccgatgtgt
    10681 tccgcatcaa catgagccat tccagccacg aggtgttgcg ccagcgcgtt gcgcagatcc
    10741 ggaccatcga gaaatgggtg gggcggccga tcggcattct ggccgatctg cagggcccca
    10801 agcttcgcgt cggccgcttc gccgccgggc cggccgaact gaagaatggc gcccgcttcc
    10861 ggcttgatgc cgatgccaca ccgggcgatg catcgcgtgt gcagctgccg catccggaaa
    10921 tcctgcagtc ggttgtcgct ggccatcgcc tgctgatcga cgatggcaag ctgtgcctca
    10981 gggtgacgga gactggtcct ggatttttcg aaacggtcgt cgaggttggc ggcccgatct
    11041 ccgaccgcaa gggcgtcagc cttcccgata cgatgctgcc ggtcggagcg ctgaccgaca
    11101 aggaccgcgc tgatctcgac gccgcgctgt ccgccaatgt cgactggatc gccctgtcct
    11161 tcgtgcagcg tcccgaggat gttgccgagg tgcgcaaggt ggcgcgaggc cgggcgctgg
    11221 tcatggccaa gatcgaaaag ccccaggcca tcgagcggct cgacgaaatc atggaaattg
    11281 ccgatgccct gatggtcgcg cgcggcgatc tcggcgttga aatgccgctt gaggtcgtgc
    11341 ccggcctgca gaagcgcatc actcgcgtcg cccgccgata tggcaagccg gtggtcgtgg
    11401 cgacacagat gctggaatcg atgatcacca gccccgcacc gacgcgagcc gaagtgtcgg
    11461 acgttgccac ggcagtgttc gaaggggctg atgcggtcat gctgtcggca gaaagcgcct
    11521 cgggcaagtt cccgctcgag gcggtctcga cgatgagccg catcgccgag caggtcgagc
    11581 gtgattccaa ctacaagatc atcatcgacg gtcagcgtgc cgatcccgag cccaccggcg
    11641 ccgacgccat cgccgccgcg gcccgcaccg tggccgaaac gctcgacctg aaggccatcg
    11701 tcgcctggac ggcctccggc gccactgccc tgcgtatcgc gcgcgagcgg ccctacacgc
    11761 cgatcatcat gctgaccaca tcagaacgca ccgcccgacg catgcagctt gtctggggga
    11821 tcaatccggt catcggggcc gatcccaccc atacggccga catggtgcgc atcggcacga
    11881 ctgcagcgcg caattccgga ctggcccagc ttggccagcg catcatcatg gtcgcgggcg
    11941 ttccattcgg gactccgggc tcgaccaaca tgctcaggat tgccttcgtc agttcggaca
    12001 gctgatggcc aagaatcatt gttactgact tttgacgtga tgggcaggcg cggggttaca
    12061 gttcccgccc ctcgacctcc ggccgcatcc ggtcaacgat ctcccgcact tccaccagat
    12121 gcggatagac ttcgagcacc ttctgatagg ctgcataggc ggccttcggc ttgccgagcc
    12181 tctgccagat cattccaagc ccggcgagcg cgctgaaatg gcgcggctcg cgcgccagaa
    12241 ccgcccggag atcccgcatc gccccatcgt aatcatccat caggaacagc agcgttgcgc
    12301 gcttggcata ggcctcggcc cagtttggtt gcagctgcag gatatgatca agcagctcaa
    12361 gcccggtatc gtaggctttc gccgacatga cctcgagcac ccgatccatc agcagattgg
    12421 tggtatcgct gcccgatcgc atccagcgct gttcaatctg cttggcgatg gggcgggctt
    12481 cattcggatc ccgggcggtg cgcaactgct tgtaaagcgc atccaaagtc accggcggcg
    12541 gcaggacctt ggcacccggc ggtagatcag acgacggcgc cggccgcccc tgctgcgccg
    12601 aagcgatgcc ttgcagcgcg acggtcagaa tgattgcaaa aaacaaccgc gccatggctc
    12661 catcctctct tgcgaggata gctccacggc gcggcgaatt caatctccgg gcgcctcagg
    12721 cggcgcggat gtcagccctg acgcgccttg aagcgcttct gggtcttgtt gatgatgtag
    12781 acacggccgc ggcgacgcac caactggttg tcacgatgcc gcttcatcag cgacttgaga
    12841 gagtttcgga tcttcatctt ggcaccactc tccggccgag ccggaacaaa aactgatacc
    12901 ggccatgcct ttggcaaaac ccgccatgcc ccttgtgagg cagaccacag ccggagagag
    12961 gttctcaata cgaatttttc tgtccggcgc aactcttttc tgatgatggg gggctggctg
    13021 ggccaagagg cggggtttcc cgcagggcga tccggtcgat caatccgtcg gcatggcaac
    13081 cgccaaaaac cccgctgcca ccgagcggca ttcatcccgg ccggcagaca aaccgtgata
    13141 cgcgctgacc ctgccttgat gatcatgata tgctgatagc atcgagggaa tggaccaggt
    13201 catgtttgat tcaacgcttc ttgtcgggaa atctgcgctg gtgaccggtg cctcatccgg
    13261 tctggggcgc catttctccg ccgttctggc gcgcgcaggt gccgaggtga cactggccgc
    13321 ccgccgcgag gtgatgctgg ccgaggctgc cggcgccatc aggcaggcag gtggccgcgt
    13381 ggtgaccgcc cggctcgatg tgacggaccc gcagacggtc gaggaggtct gcggccagcg
    13441 agccttcgac atcgtcatca acaacgcggg cgtgacccat gacggcaagg cgctgaccgt
    13501 ctcgatcgag gattggcgcc atgtcctcga gaccgacctg accggtgttt tccgcgtcgc
    13561 tcaggccgcg gccaatcgca tggtcgccga gaaccgcccg ggttcgatcg tcaatattgc
    13621 ctcgattctc ggtcatcgcg ttgccggcaa tctcgccgct tatgcagccg caaaggcagg
    13681 tgtcattcag ctcacgcgca gcctcgcgct cgaatgggcg cgccatcgga tccgcgtcaa
    13741 tgccctctgc cccggctata tcgccaccga tctcaatcgc gcattctttg cctcttccgc
    13801 cgggcaggcg ctgatcggcc gcataccaca acgcaggctg ggccagctct ccgatctcga
    13861 cgggccgctg ctcctgcttg cctccgacgc cggagccttc atgaccggca cagatatcgt
    13921 ggtcgatggc ggccatctgg ttgcctcgct ttaggatttc accatggatt tcaccatctc
    13981 gccccgcatc gaggacttcc gcagccgcat tgcccggttc gtcgaggaca aaatccttcc
    14041 cctcgaagca gatcgatcga gctatgatgc gcacgacaat atccgtctcg acctgctcga
    14101 ggcgctgcgg cagagggcgc gcaccgaagg attgtggtgc cttcagctga aaccggaaac
    14161 gggagggcag gggctcggca aggtgggaat ggccgtttgt tacgaagcca tgaaccgatc
    14221 gatcttcggc ccggtcagct tcaactctgg ggccccggat gacggcaaca tgatggttct
    14281 ggaacaggtg gcgaccccgg cccagcagga gcgctggctg gcgccaatcg ttgcgggcaa
    14341 ggttcgctct gcctttgcga tgaccgagcc tcatcccggt ggcggctccg acccatcgat
    14401 gatgctgacg accgcgacca gacggggcga ccgttatgtg atccgcggtc gcaagtggtt
    14461 cattaccggt gccggcgagg cacagcattt catcctgttg gcacggacct ccgaggatcc
    14521 gcgccggggg ctttcagcgt tcctgttcga tcgggatacg ccgggctggc ggatcgaccg
    14581 ccggatcccg atcatgggcc cggaggagca cggagggcat tgcgaactgg tgttcgacgg
    14641 ccttgagatt ccggtcagcg atctgctgat gaacgagggg gacggattga aactgacgca
    14701 gatccgtctg ggcccggcgc gcctgaccca ttgcatgcgg tggctgggtc ttgcccggcg
    14761 ctgcgtcgac attgcccgga cctacgccgc cgagcgtttt gccttcggcg aacgactggc
    14821 aaaacgcgaa agcgtccgga tgatgctggg agatcttgcc atgcagatcg agatcggccg
    14881 cctgctggtg atgaaggcgg cctgggaact cgatcgcggc ggctatgccc gcaaggaaat
    14941 ctcgatggcc aaggttcacg tcgccaacct gctgcacaag gcggcggata ccgcactcca
    15001 gatcaacgga gcgcgtggct acagcaagga taccccgctg gaatggatct accgctacgc
    15061 ccggcaggcc cggctggtcg acggggccga cgaggtgcac aagatggtgc tcgaacgcaa
    15121 ccttgccagc gagggccagg atttctggcg ctggccggtc gggcagtgac atgtggcagg
    15181 aaccgacatg aatgctgcca cggactttga cgatacgccg ctgcgggcct ttctgatgtc
    15241 gcgcttcggc gaaacaccca gctttcggct ggagcggatt gcgggtggac aatcgaaccc
    15301 gacctatttc gtcgatcatg gcgagcacag gttcgtgctg cgcaagcagc ctgacgggcc
    15361 cttgctgcgc ggcgctcatg cgatcgaccg ggaatatcgg gtgctcaccg ctctcaatcc
    15421 gaccggcgtg cccgtgccca ggccgatcct ctttcacgaa gcgccggata ttgtcggcac
    15481 gccattctac ctgatggagc gcgtcgaagg ccggatcttc gccagtggcg cgctgccaga
    15541 ggctgcccca ggcgaacgga atgcgctgtg gatggggctg gccaaggcct tggcacacct
    15601 ccatgccctg cgcccggatg aggttggcct cggcgacttc ggccggcccg gcaactactt
    15661 tgaacggcaa ctggcgcgct ggtcccggca gtggcatgag agttcgagcc agcccatccc
    15721 ggaactggac gcgcttgcgg cgtggctggc ggatcatctg ccggcggacg atggcgctgt
    15781 cagcattgcc catggcgatt atcgcatggg caatgtcatc ttccatccca ccgagccgcg
    15841 cgtggtcgcc atactcgact gggaactggc gaccctgggc catccgctgg ccgatgtcgg
    15901 gttctgtgcc atggcctggc acaccgcccc cgaggaatat ggcggcattc tcggccttga
    15961 tctggcagcc tgtgggctgc cgacgcagga acagttcgtc agctgctatg cccgcgccaa
    16021 cccatccgtt gccgagctgc aaccgtttca cctggcgttt gccctgtttc gctttgcggt
    16081 catctttgtc ggaatcgcgg accgggcccg ccagggcacc gcagcgggca cagacgctcg
    16141 atcaatcggc ccgctcgccc gccgttttgc ggttcgggcg ctggaagtga tcagcggcaa
    16201 gcactgaacc tgactgaggg cgggcgccga gatgaagccg gaccggcagc gcgggccgca
    16261 ggaatttccc gctcgagcac cgtcatacca acggcccggg gcggacccga ccggtcagag
    16321 catgcaaaaa gcgaaaaacc gcccacactt tttcgcagca tgctccaatc gcagcatgat
    16381 ctaaggctcg tgggggcact cgtatcagct ctggccgagg gctcaaatgc tgcgcgccgc
    16441 catgccgctt gtggcaggct gatcaagccg ttgatggcaa cgaccaccac tgataccaag
    16501 tcgcaaggcg aggaactcgc cgcgacttgg ttcgtttcaa aaatttctca cttttatcaa
    16561 cgagatgaga aatttttgag ttgcaccaaa gagtcatttt caatgacttt tggtatgagc
    16621 ggtccgggcc cctgacaata gagaaggggg atgtcccctt gcgggggaac cactctcaca
    16681 attccaaaga tcctgtcatg gaaggcaatg gtgggcgcga cagggattga acctgtgacc
    16741 cctacgatgt caacgtagtg ctctcccgct gagctacgcg cccgaaatgt cgtcgtcaga
    16801 ccatccggtc gggaaggccg ctctataagg tcaggccagc gggaaggcaa gagccaatcc
    16861 gccagccaat ccgccctgag ggaagattgc ccggatcagg cagccgccag cattttcttg
    16921 acctcgttga cgaggtcctt gagatggaag ggcttggaga gaaccttggc atccttcggc
    16981 gcctgcgaat ccgggttgag cgccacggcc gcaaaaccgg tgatgaacat cactttgatg
    17041 tcgggatcca gttccgttgc ccgacgcgcc agctcgattc catccatttc cggcatgacg
    17101 atatcggtca gaagaagttc aaacggttct tcgcgcaggc ggtgataggc ggacttgcca
    17161 ttgtcgaacg ctgccacgtc atagccggcg ttctgcagcg cgcgcacgag aaaccggcgc
    17221 atatcgttgt catcttcggc aaggaggatc ttggtcatgg ttgaggtccg acgcctgatt
    17281 tggcccttct tctgtttaac cccggagatg gtaaacaaaa ggtaacggag gcgccagatc
    17341 ggccacaaat ctgttatctc cgacgctgcc tgacatcgtc cggctggcca tgaccggtgg
    17401 tgatcgcgtc ggccgctctt gaaatcgtgc gttgcgcggc tagcatgcca gccatgcccg
    17461 cagaactgcc tcccgacgaa gccggctgct tgcccggatg ctccgagttc gatccgccgt
    17521 tcgaactggt cgagcccgtt gctgtttcag ctcccgtact gttcaattca ccgcattcgg
    17581 gcgtgacctt tccaccgtcc ttcctggcgc agactccgct ctctcaggcc gatctgtgcc
    17641 ggtccgccga tcttcacgtc gaccgctttc ttgatggacc gctggcgctc gggatgtcgg
    17701 tgttgcgggt caatattccc cgctcattcc ttgatctcaa ccgcgaagcc catgagctgg
    17761 atccgacgat gttcgccgga cggctgccgc cgccggtcaa cagcgggtcg ctgcgggtca
    17821 gcagcggtta cggaaccatt ccccggctgg tggctgaagg ggtcgcgatc tatcgccggc
    17881 cgttgccggt tgccgaggcg ctgcggcgaa tcgactgcta ttaccggccc taccatcgcc
    17941 tgctcgccac acatctggca cgcctgcgac aggcgttcgg cagtgcgatc ctgatcgact
    18001 gccattcaat gccatcgcac agcgggggcc atgcgctcgc agacatcgtg cttggcaatc
    18061 gcaatggccg cagctgctct ccggagctga tgcggcttgc attccactgg ttcgaatcgc
    18121 tgggcctctc ggtcgcctgc aatacgccct atgccggagg gtttatcacc gagcattacg
    18181 gcaaacctgg tttgggccag catgcgctgc agatcgaggt gcatcgcgcg ttatatatgg
    18241 atgagaagag ctatgtgccg catgaggggc tggcgcgcct cgccgatgcg atgaccggct
    18301 ttgccgccag gctggttgat tatttcaatg ggcaggaaag gccagcgccg caatgggccg
    18361 ccgaatgaaa aaaggccacc cgcgaagggt ggcccaagtc tagggaggaa acgcccaaag
    18421 gagggcacgc aaggca
//
LOCUS       DAOMXG010000032        17507 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_1851_length_17507_cov_10.267763, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000032 DAOMXG010000000
VERSION     DAOMXG010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 17507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17507
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1851_length_17507_cov_10.267763"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..100
                     /locus_tag="PLQ11_03750"
     CDS             <1..100
                     /locus_tag="PLQ11_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007824539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HRK24049.1"
                     /translation="LQVAFLPLPVIPHLKISDKGLVDVDAFALIGA"
     gene            156..1028
                     /locus_tag="PLQ11_03755"
     CDS             156..1028
                     /locus_tag="PLQ11_03755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01459 family HAD-type hydrolase"
                     /protein_id="HRK24050.1"
                     /translation="MSHAPVPMIERFSSISDRYDGIICDVWGVLHNGARVTRQAAEAL
                     AAARRRGLKVVMLTNAPRMPENVAQFIDQLGGAPEAFDAIIASGGVTRELIAAEGARR
                     YHHIGPARDTNIFTGLAGRPAPLSEADYVVCTGLIDDETETAETYRAQLEAMRQRGLT
                     MICANPDIVVERGHRLIPCAGAIADLYEKIGGRVTWVGKPHGLVYDIARREIERLLGR
                     PVEKSRILGIGDALRTDVAGAAGYGIDCLFVLDGIHSADLGALGRDFSSAAVNALLER
                     STVRPTYATVALVD"
     gene            complement(1001..1408)
                     /locus_tag="PLQ11_03760"
     CDS             complement(1001..1408)
                     /locus_tag="PLQ11_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003604011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRK24051.1"
                     /translation="MPVNPSMSVLLVDDYAIMIRILRNLLRQIGIENVDEASDGGEAL
                     GMLQKRRYDLIISDWNMQPMSGLELLGAVRADPVHGDVPFIMVTAESKTNNVLAARMA
                     GVSTYIVKPFNAQTLRSRIEAVCTRQSTSATVA"
     gene            1595..2539
                     /locus_tag="PLQ11_03765"
     CDS             1595..2539
                     /locus_tag="PLQ11_03765"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HRK24052.1"
                     /translation="MSRPFLRLADPNTLPENLRHPVLAIGNFDGVHRGHQALLELARQ
                     KADAAGVPAAALTFFPHPRAFFRPAEPQFRLTPPDLQAERIALSGLDGMIVLTFGSAL
                     AELAAESFVADILIGRFGISGVVVGADFHFGKARSGTPAFLSAAGQRHGFSVTMVGDV
                     ADGGQRVSSSRIRAALAAGDPALANRLLGYEWTVRARVEHGAKRGRTLGYPTANMALD
                     PACGLSEGVYAVRIRIDGAWRPGVASFGRRPMFDNGAPLLETHVFDFSGDLYGRDLDV
                     AFVAFLRPEMAFEGVEALVRQMDADSGAARTALAEPKI"
     gene            2728..5910
                     /gene="ileS"
                     /locus_tag="PLQ11_03770"
     CDS             2728..5910
                     /gene="ileS"
                     /locus_tag="PLQ11_03770"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HRK24053.1"
                     /translation="MTDTAKDYSQTLFLPETAFPMRAGLPAMEPKILERWVRNDLYGQ
                     LRASAAGRPKFVLHDGPPYANGHIHIGHALNKILKDMVTRAQQMSGFDSNYVPGWDCH
                     GLPIEWKIEEEYRAKGREKPDFSDGTAINAFRKECRSFAEHWLTVQREEFKRLGVIGD
                     WDNPYSTMAFHAEAQIAREIMKFAENGLLYRGSKPVMWSVVEKTALAEAEVEYHDHQS
                     DTVYVAFPVAFAPDRELDLLVKIYHGAALERGRNVTETVIRSELQSCKLTDCSVVIWT
                     TTPWTLPGNRAIAYSHRLSYGIYEVSQLLEGSYAKIGTRFMVADNLAGELFEKMKAES
                     IRLRDVVAYELDAVRCTHPLAEFHPFYAALKLPLLDGDHVTDDAGTGFVHTAPGHGRE
                     DFDLWMAAKSKLEAPLVASARIDTTIPYTVDEDGAFTRDAPGFTGERVLTDKGEKGGA
                     NEAVIKALIGTGTLVARGRLKHQYPHSWRSKKPVIFRNTPQWFIAMDREFVPASSSPL
                     AGEGREGGGSTSAAPGSATPLTNSPPQGGRGNSLSLRAVALDAIRQTQWVPAAGENRI
                     TGMIENRPDWVVSRQRAWGVPITVFVHKKSGEILKDAAVNVRIAEAFEKEGADAWFAD
                     KTGARFLGPSHDPADFDKVSDVLDVWFDSGSTHSYVLNTPKHFPGLADVHRKVDGGAD
                     TVMYLEGSDQHRGWFHSSLLESCGTRGRAPFDVVLTHGFCLDEKGQKMSKSLGNVVAP
                     QAVIKESGADILRLWVAASDYSDDLRIGEHILKNFVETYRKMRNTLRWMLGSLAHFRP
                     DQAVAHGDMPELERLMLHRLAELDAEVRPAYAAFDYKKVVAVLAQFMNTELSAFYFDI
                     RKDTLYCDPASSKARRAALTVIDRLFETLTVWLAPILSFTAEEAWLARHGDSPQVSVH
                     LQQFPALPAAWQDAVLAAKWDKVRAVRRVVTGALEIERAQKRIGSSLEAAPQVFITDA
                     ALSEAVHGVDMAEVAITSGLTIIDSEGPDDAFRLAEVAGVSVVPARAGGIKCARSWKY
                     FDPASADPAYPDVTGRDAAALREWAARAGQ"
     gene            5907..6383
                     /gene="lspA"
                     /locus_tag="PLQ11_03775"
     CDS             5907..6383
                     /gene="lspA"
                     /locus_tag="PLQ11_03775"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017485654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HRK24054.1"
                     /translation="MTNGRSPFRAGLIGALTTLALDQASKLWLLFGLGLPAHYPVSLA
                     PWFDLTVVWNRGISYGLFQQDSELGRWALIAFKLVAAIGLVVWMGRCTSRATAVGLGL
                     IAGGAVGNAIDRIAYGAVFDFAHLHWGTFSWYVFNLADAAIVAGVPLLLYPEPKSR"
     gene            6452..7099
                     /locus_tag="PLQ11_03780"
     CDS             6452..7099
                     /locus_tag="PLQ11_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24055.1"
                     /translation="MKLLRNGALAGSAIALVLVAASPASAQLLSDPLDEVLSSIGLKD
                     RERDPIDYRERAPLVVPPSGAKLRSPEAPAGANNPAWPLDPDVAARRAKAAESKTMMA
                     PPSDRDPSQGGLVRGAEGHKRNARAGVPTSNSPGYTGEEPSPMRSLSPDQVGRILASS
                     PESSQPSGIEPQRQYLTEPPKGYRRAAAGAPVRATVEPMPSGNDSVQMNVLQPRR"
     gene            7204..8481
                     /locus_tag="PLQ11_03785"
     CDS             7204..8481
                     /locus_tag="PLQ11_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020180997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRK24056.1"
                     /translation="MSHDHAPELSHFQLPNGLDVVVIPDRRAPVVTHMVYYRNGAADD
                     PHGKSGIAHFLEHLMFKGTEKHPDGAFSDLVSDLGGQENAFTGHDYTAYYQRVAREHL
                     QKMMELEADRMTGLVLAEDQVLSERDVILEERKMHVDADPASQLYESLQATLFTHHPY
                     GTPIIGWQHEIEQLTREDALAYYRRFYTPENAILIVAGDVTEERVRALAEKTYGRIAR
                     RNEAPRRQRPQEPPPAAERLVTLTDAKVEQPAVYRMYLAPSHGTAKGNDAHALEVLCQ
                     VLAGSSASLMYRDLVVERSLAVNVSSWYRNDALDQGYFGLYAVPRDGVGLDDLDREID
                     AILAQVMANGFSEAVLARGKTRLIADLVYAQDSQAHLARIYGGTLATGGSIADVRDWS
                     DRIDQVTLDDIRRVAAACLNRARVVRGHLRKAA"
     gene            8484..9773
                     /locus_tag="PLQ11_03790"
     CDS             8484..9773
                     /locus_tag="PLQ11_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRK24057.1"
                     /translation="MASPKRKVAVAPQTRIDRVVSPSGIEAWLVEDYTVPLISMDFAF
                     VGGGALDPAPLAGLSHLMAGLLDEGAGPYRGQDYQERLEDKAIEISFSSNRDRIEGSL
                     RTLDRHGEAAFDMLALAVNQPRFDQDAIDRVKAQVMASLKREETDPDALAWKAFRARA
                     FGDHPYGRGDKGTPASLSTITRADLVTAHGRRLARDRLKIAVVGAIDAETLGRALDRI
                     FGGLPASMPADIVADIVPAGLGSVVVSPLAVPQSTLALGRPGLHRLDPDFMAAYVVNH
                     ILGGGSFTSRLWTEVRERRGLAYSVWSQLSTSRHSAMLLAGTATSNERVAESLAIIRA
                     EFARMAEKGPSGRELDRALQYLIGSYALRFDTSRKIAGHLVEIQVEDLGIDYIAMRNS
                     RMAAVTLADTRRVAARLIGDGGLLVAVAGQPEGIADT"
     gene            9805..10776
                     /gene="sppA"
                     /locus_tag="PLQ11_03795"
     CDS             9805..10776
                     /gene="sppA"
                     /locus_tag="PLQ11_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HRK24058.1"
                     /translation="MSLDADYLIDRRRLRRKLTLWRVLACAAGAAALIALGLMSGGKE
                     LVDSQSAHIARLTVSGLITSDKPMLEAIRKVKDAASAKAAIVSINSPGGTTTGSEALF
                     RELRDLAVAKPTVAVVTGTAASGAYVAAIGTERIFAPETALIGSIGVIVQYPNFVKTL
                     ELMGVKVEAVRSSPLKAMPSGVEPTLPEARAALEASVADSYAWFKGLVKDRRSLSEEE
                     LVKVSDGRVFTGRQSVPLKLVDQIGSEKEAIAWLESEKSIAKSLKVRDYKRVDPNDRL
                     GLFSLAAGIADFFGHEEFAASLRTTGRGIAAQSLDGLLSVWQPRIEN"
     gene            10816..11118
                     /locus_tag="PLQ11_03800"
     CDS             10816..11118
                     /locus_tag="PLQ11_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HRK24059.1"
                     /translation="MIKSELVLKIAEQNPHLYQRDVENMVNSILDEIVDALKRGDRVE
                     LRGFGAFSVKRRDARVGRNPRTGSKVAVAQKVVPYFKSGKEMRQRLNKQPARAKKS"
     gene            11257..11577
                     /locus_tag="PLQ11_03805"
     CDS             11257..11577
                     /locus_tag="PLQ11_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HRK24060.1"
                     /translation="MLAFLRLLLLAPIAVVLIMLALANRTPVPLSFDIVGLEAQSVTV
                     PLYVALLGAVMLGILIGGIACWIGQGKHRKAAREAGRAADQARAEAERLKLAASPLAA
                     LPPR"
     gene            11596..12255
                     /locus_tag="PLQ11_03810"
     CDS             11596..12255
                     /locus_tag="PLQ11_03810"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HRK24061.1"
                     /translation="MPLSVKICGLSTPATVEAALGAGADMIGFVFHPKSPRFVSPDAA
                     ARLARAARGRVEIVALTVNMTPEAAEDLANVLKPDWFQLHGDEAPGLAEATRQATGCR
                     ILRAIGVETVADVTRAGIHALAADRLLIDAKAPKDAAYPGGHGRPFDWNILAALDPGL
                     PFMLSGGLTPETVGAALATVRGLGVGIIGVDVSSGVETAPGVKDAGKIHAFIAACRAA
                     G"
     gene            12323..13540
                     /gene="trpB"
                     /locus_tag="PLQ11_03815"
     CDS             12323..13540
                     /gene="trpB"
                     /locus_tag="PLQ11_03815"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003608475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HRK24062.1"
                     /translation="MNAPDKLNSFRSGPDERGFFGIFGGRFVAETLMPLVLDLERAYG
                     EARNDPAFREEMVRMGRDYIGRPSPLYFAERFTRHFGGAKVYFKREELNHTGAHKVNN
                     VLGQILLALRMGKTRIIAETGAGQHGVATATLCARYGLKCVVYMGAVDVERQKPNVFR
                     MRMLGAEVVPVQSGAKTLKDAMNEALRDWVTNVHDTFYCIGTVAGPHPYPAMVRDFQS
                     VIGIETRAQMLDAEGRLPDSLVAAVGGGSNAIGLFHPFLDDPSVEIYGVEAAGHGIAS
                     GAHAASMAGGRPGVLHGNRTYLLMDDDGQIKDAHSISAGLDYPGVGPEHSFLHDVKRV
                     RYEPITDDEALAAFQTCSKLEGIIPALESAHALALAEKLAPQKPKDHLMVINVSGRGD
                     KDIFTVAEHLGGM"
     gene            13540..14373
                     /gene="trpA"
                     /locus_tag="PLQ11_03820"
     CDS             13540..14373
                     /gene="trpA"
                     /locus_tag="PLQ11_03820"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HRK24063.1"
                     /translation="MTKSRIADRFAACKAENRAALVTFTMAGDPDFATASAILAGLPA
                     AGADVIELGMPFSDPMADGPAIQLAGQRALAAGQTLAKTLGMVRSFRASDPATPIVLM
                     GYYNPIYIYGVGRFLKEAVAAGVDGLIVVDLPPEMDDELCLPALKAGLHFIRLATPTT
                     DDKRLPTVLKNTSGFVYYVSITGITGAATPDFSKVANAVARIKQHTPLPVAVGFGVKT
                     PEHAMAIAGGADGVVVGSALVDAVRTSLSDGKASAGTVAAVTDLVSSLAAAVRATRRD
                     L"
     gene            14426..15283
                     /gene="accD"
                     /locus_tag="PLQ11_03825"
     CDS             14426..15283
                     /gene="accD"
                     /locus_tag="PLQ11_03825"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018042113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HRK24064.1"
                     /translation="MNWITNVVRPRINSLLKRETPENLWIKCPDTGQMVFHKDVEANL
                     FVIPGSEHHLKMGARARLASMFDGGDFEEIAVREMPVDPLKFRGKDRYIDKLKEAKAA
                     TGLADAVKIGYGQVDGLPVTIGAHDMDFIAGSLGMAAGEAVITGLETAARRGTPFVLF
                     TASGGARMQEGILSLMQMPRTTVAVRRLRAAKKPYIVVLTHPTTGGVTASYAMLGDVH
                     LAEPGALIGFAGPRVIEQTVREKLPDGFQRAEYLKDHGMVDMVVHRKDMKVTISRLCR
                     VLMKAPAAA"
     gene            15298..16617
                     /locus_tag="PLQ11_03830"
     CDS             15298..16617
                     /locus_tag="PLQ11_03830"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HRK24065.1"
                     /translation="MKLSEVYLQRFQALHPVLIDLSLGRIERLLARLGHPERRMGPVI
                     HVGGTNGKGSTIAFLRAMLEAAGKRVHVFTSPHLVAFNERIRLAAPGGGQLATEEQLV
                     AAFEHVEAVNGADPITFFEITTAAAFHLFATHPADITLVEVGLGGAFDATNVFEQPAA
                     AVITPVSLDHKEHLGDTVEKIATTKSGIFKRGAPAIIAPQDETPSRVLQRAAERIGAR
                     PMVGGEDFALHEEHGRLVYQDAEGLIDLPLPRLPGRHQHINAATAIATLRTVFGAGFP
                     VAAIEKGLQQVEWPARLQHLTGQLAALTPSGAELWLDGGHNADGARALAAAMADLEER
                     NPRPLVVVLGLMARKDADAILEPFRGLAQEFYAVDIPGNEKTARKADDLAILARDLGL
                     PCAVAGDVAQTLRFLSQRSWPRPPRILIAGSLYLAGEVLKADGSLPK"
     gene            complement(16669..16992)
                     /gene="trxA"
                     /locus_tag="PLQ11_03835"
     CDS             complement(16669..16992)
                     /gene="trxA"
                     /locus_tag="PLQ11_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HRK24066.1"
                     /translation="MSNAVKVTDASFEADVLKSAEPVVVDFWAEWCGPCRMIAPSLDQ
                     IAVEMAGKVKVVKVNIDENPNVAAKLGIRSIPTLMVFKGGKLAATKVGAAAKGDLQRW
                     IQASI"
     gene            complement(17073..>17507)
                     /locus_tag="PLQ11_03840"
     CDS             complement(17073..>17507)
                     /locus_tag="PLQ11_03840"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRK24067.1"
                     /translation="EAAASWLADRAPRLSTQARAGLLEPVLALLNEPLGQRLFGPAAE
                     AEVAVAGAIPHPDGRRLRVAGRIDRLLVEPDRVTVVDFKTGRPPPAGAAMPGHIVQLA
                     LYAALLAQLYPDRRIEAALVWIQQGRIDRIDAKCLDDAITLL"
BASE COUNT         3062 a         5496 c         5622 g         3327 t
ORIGIN      
        1 cctgcaggtg gcctttctgc cgctgccggt gatcccgcac ctgaagattt ccgacaaggg
       61 cctcgtcgat gtcgatgctt ttgcgctgat cggggcttga atcagcgccc gcgaccatgc
      121 cagtaggtca ttgctcatcc tgtcaaggct tgccgatgtc ccatgctccc gtgccgatga
      181 tcgaacgctt ctcgtccatt tcggaccgtt atgatggcat catctgcgat gtgtggggcg
      241 tgctgcataa cggcgcccgg gtaacacggc aggccgccga ggcgctggcg gccgcgcggc
      301 gtcgcggcct caaggtggtg atgctgacca acgctccccg catgccggaa aatgtcgccc
      361 agttcatcga tcagctcggc ggagcgccgg aagcgtttga tgccatcatc gcctcgggtg
      421 gcgttacgcg cgaactgatc gccgctgaag gcgcccggcg ctaccatcac atcggtccgg
      481 ctcgcgatac gaacatcttc accggtcttg ccggccggcc cgctccgctt tccgaagccg
      541 attatgttgt ctgcaccggc ctcatcgacg acgagaccga gaccgccgag acctatcggg
      601 cccaactgga agcgatgcgc cagcgcgggc tgaccatgat ctgcgccaat cccgatatcg
      661 tggtcgagcg cgggcatcgg ttgattccat gcgccggcgc catcgccgat ctctatgaaa
      721 agatcggcgg ccgcgtgacc tgggtcggca aaccacatgg cctggtctac gatatcgcac
      781 ggcgcgagat cgaacggctg ctgggccggc cggttgaaaa atcccgcatt ctcgggatcg
      841 gcgatgcact gcgcaccgat gttgccggtg cagcgggcta cggtatcgat tgtctgttcg
      901 tgctggacgg catccattcg gcggatctgg gcgcgctggg ccgtgacttt tcttctgcgg
      961 ctgtcaatgc tctgctggag cgatcaaccg tcagaccgac ttatgcaacc gttgcgctgg
     1021 tggactgacg cgtgcacacc gcctcgatcc tggagcgcag cgtctgggca ttgaatggct
     1081 tgacaatata ggtgctgacg ccggccattc gggccgccag cacgttgttt gtcttggatt
     1141 cagccgtgac catgatgaat ggcacatcgc catgcaccgg atctgcccgg acagcgccca
     1201 acagttcaag gccagacatc ggctgcatgt tccagtcgga aatgatcagg tcgtagcggc
     1261 gcttttgcag catgccgagc gcttccccac catcggatgc ctcgtcaaca ttctcgatgc
     1321 cgatctgcct gagcaggttg cgaagaatgc ggatcatgat ggcgtagtcg tccaccaaga
     1381 ggacggacat cgacggattg actggcatta tccgctttat ccctcgttgc cgagctccct
     1441 gcgcaagccc ttgtctgtgc accatctatt ctgcttcggc cgagggtatt cagcagaggt
     1501 tacctttcat ttaaaccaag accattggac gcggccggga ttgcctgata gaggggccgg
     1561 accctaaccg gccacaatcc cgcacgcgca gttcatgagc cgcccgttcc ttcgcctcgc
     1621 cgacccaaac accctgccgg agaacctcag acatccggtg ctggcgattg gcaatttcga
     1681 cggggtgcac cgcgggcatc aggccctgct ggaactggcg cgccaaaagg ccgacgcggc
     1741 aggcgtgcca gcggcggcgc tgacgttttt tccgcatccc cgtgccttct tcaggccggc
     1801 agaaccgcag tttcgcctga caccgccgga tctacaggcc gagcggatcg cgctgtccgg
     1861 tcttgatggc atgatcgtcc tgacattcgg ctcggctctg gcggagcttg cggccgaatc
     1921 ctttgttgct gacatcctga ttggacggtt cggcatcagc ggggtggtgg tcggcgctga
     1981 tttccatttc ggcaaggcaa ggagcggcac gcccgccttc ctcagcgctg ccgggcaacg
     2041 gcatggcttt tccgtgacca tggtcggaga cgtcgccgac ggtggccagc gggtttcctc
     2101 atcgcgtatc cgcgccgcat tggcggcagg cgatccggcg ctcgccaatc gcctgctcgg
     2161 ctatgaatgg acggtgcgcg ccagggttga gcacggcgcc aagcgcgggc gaaccctcgg
     2221 gtatccgacc gccaacatgg cgctcgatcc tgcctgcggg ctcagcgaag gggtctatgc
     2281 cgttcgtatc cgcatcgacg gcgcctggcg tccgggtgtc gccagcttcg gccggcggcc
     2341 gatgttcgac aacggcgcac cgctgctcga aacccatgtc ttcgactttt ccggcgatct
     2401 ctatggccgc gacctcgacg tggcgtttgt cgccttcctg cggccggaga tggcattcga
     2461 gggcgtcgag gccctcgtcc gccagatgga tgccgacagc ggcgcagcac gcacggcgct
     2521 cgccgagcca aaaatctgac cggccaccgc tttgaaaagc cgcgccgtct tgctatgagg
     2581 cgccatggat ttgcacgtca tacggcgaat tacgatcccg gcccgtcgct gagcgctccg
     2641 ctcgcggcgt gaccgggttt tgccgccgcc gccaacgcgc tgccgttgcg cgcttgttcc
     2701 gttcgttctt tcgagactgt ttccgtgatg accgacactg ccaaggacta cagccagacc
     2761 ctgtttctgc ccgaaaccgc atttcccatg cgcgccggcc tgccggctat ggagccgaaa
     2821 attctcgaac gctgggtcag gaacgatctg tatggacagc tccgcgcttc cgccgcgggg
     2881 cggccgaagt tcgtgctgca tgatggcccg ccttacgcca atggccacat ccacatcggc
     2941 catgcgctca acaagatcct caaggacatg gtcacccgcg cccagcagat gagcgggttc
     3001 gattccaact acgtgcccgg atgggactgc cacggcctgc cgatcgagtg gaaaatcgag
     3061 gaagagtatc gcgccaaggg ccgggaaaag cctgactttt ccgatggcac cgccatcaac
     3121 gccttccgca aggaatgccg ctccttcgcc gagcactggc tcactgtgca gcgcgaggaa
     3181 ttcaagcggc tgggcgtgat cggcgactgg gacaacccct attcgacgat ggccttccat
     3241 gccgaggcgc agatcgcccg tgagatcatg aaattcgccg agaacggcct gctctaccgc
     3301 ggatccaagc cggtgatgtg gtcggtggtg gaaaagaccg cactggccga ggccgaggtc
     3361 gaataccacg accatcagag cgatacggtc tatgtggcgt tcccggtagc ttttgctcct
     3421 gaccgcgagc ttgatctact agttaaaatt tatcacggtg ccgctcttga gcgtggaaga
     3481 aatgtcactg aaacagtaat cagatccgaa cttcaatcat gcaagttgac tgattgctcg
     3541 gtggtcattt ggacgactac gccttggacc cttccaggga atagggcaat tgcctactcg
     3601 caccgcttga gttacgggat ttatgaggtt tcccagcttc tggaaggcag ttatgcgaaa
     3661 attggcaccc gttttatggt tgctgacaat cttgcgggtg agcttttcga aaaaatgaag
     3721 gccgaaagca ttcggcttcg cgacgtggta gcatacgagc ttgatgccgt tcgctgcacg
     3781 catccactcg cagaatttca tccattttac gcggcgttga agttgccact tcttgatggc
     3841 gaccatgtca ctgacgacgc gggtaccggc tttgtccaca ccgctccggg gcatggacgt
     3901 gaggattttg atctttggat ggccgctaag tccaaactcg aagctcctct tgtcgcatcg
     3961 gctcgcatcg acaccacaat cccctatacg gtggacgagg acggcgcctt caccagggac
     4021 gcgccgggct tcaccggtga gcgcgtgctc accgacaagg gcgagaaggg cggcgccaac
     4081 gaggcagtga tcaaagcgct gatcggcacc ggcacgctgg tggcgcgcgg ccggctgaag
     4141 catcagtatc cgcattcctg gcgctcgaag aagccggtga tcttccgcaa tacgccgcag
     4201 tggttcatcg ccatggaccg ggagttcgtt cctgcttcct cctcccccct tgcgggggag
     4261 ggcagggagg ggggtggttc cacatccgct gcgcctggtt cggccacccc cctcaccaac
     4321 tctcccccgc aaggggggag agggaattcc ctctccctcc gcgccgtcgc gctcgacgcg
     4381 atccggcaga cgcaatgggt gccggcggca ggcgagaacc gcatcaccgg catgatcgag
     4441 aaccggcctg actgggtggt gtcgcgccag cgcgcctggg gcgtgccgat caccgtgttc
     4501 gtgcacaaga aatccggcga gatcctcaag gatgcggcgg tcaatgtccg catcgccgag
     4561 gcgttcgaga aggagggcgc cgatgcctgg ttcgccgaca agaccggcgc ccggttcctc
     4621 ggcccgtccc atgatccggc cgatttcgac aaggtgagcg atgtgctcga tgtgtggttc
     4681 gattcaggtt caacgcattc ctatgtgctc aacacgccca aacacttccc cggccttgcc
     4741 gatgtgcatc gcaaggtcga cggcggcgcc gatacggtga tgtatctcga agggtcggac
     4801 cagcatcgcg gctggttcca ttcctcgctg ctggaaagct gcggcacacg cggccgcgca
     4861 ccgttcgacg tggtgctgac gcatggcttc tgcctcgatg aaaaggggca gaaaatgtcg
     4921 aaatcgctcg gtaatgtggt ggcgccgcaa gcggtcatca aggaatcggg cgccgacatc
     4981 ctgaggctgt gggtggcggc gtctgattat tccgacgatc tcaggatcgg cgagcacatc
     5041 ctcaagaact tcgtcgagac ctaccgcaag atgcgcaaca cgttgcgctg gatgctcggc
     5101 tcgctcgccc atttccggcc tgatcaggcg gttgcccatg gggacatgcc ggaactcgaa
     5161 cggctgatgc tgcatcggct cgccgaactc gatgccgagg tgcgacccgc ctacgctgcc
     5221 ttcgattaca agaaggtggt tgcggtgctg gcgcagttca tgaacaccga actgtcggcg
     5281 ttctatttcg atatccgcaa ggatacgctc tactgcgatc cggcctccag caaggcgcgc
     5341 cgcgcggcgc tgacggtgat cgaccgcctg ttcgagacgc tgacggtctg gcttgcgccg
     5401 atcctgtcgt tcaccgccga ggaagcctgg cttgcccgcc atggcgattc tcctcaagtc
     5461 agcgtgcatc tgcaacagtt tccggcccta ccggcggcct ggcaggatgc ggtgctcgcc
     5521 gccaagtggg acaaggtgcg cgcggtccgc cgtgtggtca ccggcgcgtt ggaaatcgaa
     5581 cgcgcgcaga agcggatcgg ctcgtcgctg gaagcggcgc cacaggtctt tatcaccgat
     5641 gcggcactgt cagaggccgt gcacggggtc gatatggcgg aggtggcgat cacctccggg
     5701 ctgacgatca tcgacagcga gggaccggac gatgccttcc gcctggccga ggttgcggga
     5761 gtttcggttg ttccagcacg agcgggcggc atcaaatgcg cccggtcgtg gaagtatttc
     5821 gatcctgcga gtgccgatcc cgcttatccg gatgtcaccg ggcgcgatgc ggcggcttta
     5881 cgcgaatggg cagcccgggc cggccaatga cgaatggccg ctcgcccttt cgtgccggtc
     5941 tgatcggcgc gctgacaacc ctggcgctcg atcaggccag caagctgtgg ctgctgttcg
     6001 gtctcggact gccggcgcat taccctgtct cgctggcgcc gtggtttgat ctcaccgtcg
     6061 tgtggaaccg cggcatctcc tacgggctgt tccagcagga cagcgaattg ggccggtggg
     6121 ccctgatcgc tttcaagctg gttgcggcga ttggccttgt ggtgtggatg ggccgctgca
     6181 ccagccgggc cacggctgtc ggccttggtc tgatcgccgg cggagcggtc ggcaatgcga
     6241 ttgatcgcat cgcctacggc gctgttttcg attttgccca tctgcattgg ggcacgttca
     6301 gctggtatgt ctttaacctt gctgatgccg ccatcgtcgc tggcgttccg ctgctgctat
     6361 acccggagcc gaaatcgcgc taaagattgc cgccaaattg cccaactgca gttgcagaag
     6421 ggtaactgga cctgcaagca aggggtgtct gatgaaactg ctgcgtaacg gagccctggc
     6481 gggttcggcg atcgcgctgg tattggttgc ggcttcgccc gcctctgccc agttgctgtc
     6541 cgatccgctg gatgaggtac tcagttccat cggcctgaag gatcgcgagc gcgatccgat
     6601 cgactatcgc gaacgcgcgc cgctggtggt tccgccctcg ggggccaagc tgcgcagtcc
     6661 cgaagccccg gccggggcca ataaccctgc ctggccgctc gatcctgatg tcgctgcccg
     6721 ccgcgccaag gcggctgaat ccaagaccat gatggcccca cccagcgacc gtgatcccag
     6781 ccagggcggt ctggtgcgtg gcgccgaagg acacaagcgc aatgcccgcg ccggcgttcc
     6841 gacctccaac agccctggtt ataccggcga ggagccgagc ccgatgcgct cgctttcacc
     6901 agaccaggtg ggtcgcatcc tggccagttc gccagagagc tcccagccga gtggcatcga
     6961 gccgcagcgt cagtatctga ccgagccgcc gaagggctat cgccgtgctg cggccggcgc
     7021 gccggttcgg gccacggtcg aaccgatgcc gtcgggcaat gacagcgtcc agatgaacgt
     7081 tctgcagcct cgccgctgaa acgggcgccc cagtttctga ctgcatcttg aagccggaag
     7141 cgatttccgg ctcaattgcg ctgattccct ggctggctgg cagggacatt ttccgaggtt
     7201 gccgtgtcgc acgaccatgc ccccgaactg agccacttcc aattgcccaa cgggcttgat
     7261 gtggtggtga ttccagaccg gcgcgcgccg gtggtgaccc acatggtcta ttatcgcaac
     7321 ggagctgcgg atgatccgca tggcaagtcc ggtattgccc attttcttga acatctgatg
     7381 ttcaagggca ccgaaaaaca tcctgacgga gccttttccg atcttgtttc cgaccttggc
     7441 ggccaggaaa acgctttcac cggccatgat tacaccgcct attaccagcg cgtggcgcgc
     7501 gagcatctgc agaagatgat ggaactcgag gccgaccgga tgaccggtct ggtgctggcc
     7561 gaggatcagg tcctgtccga gcgcgatgtc attctcgaag agcggaagat gcatgtggat
     7621 gccgatcctg catcccagct ctacgaatcc ttgcaggcga cattgttcac ccatcatccc
     7681 tacggcacgc cgatcattgg ctggcagcac gaaatcgagc agctgacgcg cgaggatgcg
     7741 ctcgcctatt accgccggtt ctacacgccg gagaacgcca tcctgatcgt ggccggcgat
     7801 gtgaccgaag agcgcgtgcg cgcccttgcc gaaaagacct atggcaggat cgcccgccgc
     7861 aacgaagcgc cccggcgcca acgtccgcag gagccgccgc cggcagccga acggctggtc
     7921 acgctgaccg atgcgaaggt cgagcagccg gccgtctacc gcatgtatct cgccccttcg
     7981 catggcacag ccaagggcaa tgacgcccat gcgctggagg tgctgtgtca ggtccttgcg
     8041 gggtccagcg ccagcctgat gtatcgggat ctggtggtcg agcgttcgct cgcggtcaat
     8101 gtctcgtcct ggtatcggaa cgacgcgctc gatcagggct atttcggcct ctatgccgtg
     8161 ccgcgcgacg gcgttggtct tgatgatctc gaccgcgaga tcgatgccat tctggcgcag
     8221 gtgatggcca acggtttcag cgaggccgtt ctggcccgcg gcaagacccg gctgatcgct
     8281 gatctcgtct atgcgcagga cagccaggcc catctcgccc gcatctacgg cgggacgctg
     8341 gcaacgggcg gcagcattgc cgatgtgcgc gactggtccg accggatcga tcaggtgacg
     8401 ctggatgaca tccggcgggt tgctgccgcc tgcctcaatc gtgcacgcgt cgtgcgcggc
     8461 cacctcagga aggcagcgtg accatggcat cgccaaagcg caaggttgcc gtcgcgccgc
     8521 agacccggat cgatcgcgtt gtctcgccgt cgggcatcga ggcctggctg gtcgaggatt
     8581 atacggttcc gctgatttcg atggatttcg cctttgtcgg cggcggtgcg ctcgatccag
     8641 cccctctggc agggctgagc cacctgatgg ccgggctcct cgatgagggc gccggccctt
     8701 atcgggggca ggattaccag gagcggctcg aggacaaagc gatcgaaatc agcttttcct
     8761 ccaatcgtga ccgtatcgag ggaagtctgc gcactctcga ccggcacggc gaggcggcat
     8821 tcgacatgct ggcgctggca gtcaaccagc cacggtttga ccaggacgcc atcgaccggg
     8881 tcaaggcaca ggtcatggcg tcgctgaaac gcgaggaaac cgaccccgac gccctggcct
     8941 ggaaagcgtt ccgcgcccga gccttcggcg atcatcccta tgggcgcggc gacaagggca
     9001 ctccggcgag cctgagcacg atcacccgcg ccgatctggt gacggcccac ggtcggcggt
     9061 tggcgcgcga caggctgaag atcgctgtgg tgggggccat cgatgccgag accctcggtc
     9121 gcgcgcttga ccggattttt ggcggcctgc cagcttcaat gcccgccgat atcgttgccg
     9181 atattgtgcc ggcgggtctg ggatcggtcg tggtcagccc gctcgctgtg cctcagtcca
     9241 cgctggcact cggacggccc ggcctgcacc ggcttgatcc cgacttcatg gccgcctatg
     9301 tcgtcaacca cattctcggc ggcggttcgt tcacgtcgcg gctttggaca gaggtgcgcg
     9361 aaaggcgcgg tcttgcctat tcggtgtggt cgcagctttc cacctcccgc cattctgcca
     9421 tgctgctggc cgggaccgcc accagcaacg agcgggtggc cgagtccctc gccatcatcc
     9481 gcgccgaatt tgcccgcatg gccgagaagg ggccgagcgg ccgcgagctt gatcgggcgc
     9541 tgcaatatct gatcggttcc tatgcgctgc gctttgatac ctcccgcaag atcgccgggc
     9601 acctggtcga gatccaggtc gaggatctgg gaatcgacta tattgccatg cgcaattccc
     9661 gcatggcggc ggtgacgctg gccgataccc gccgggtggc ggcgcgcctg atcggcgatg
     9721 gcgggctgct tgtggcggtg gccggccagc ctgaagggat cgccgataca tgaagaccag
     9781 gatggatcac gtgggggcgg gccgatgagc ctggacgccg attatctgat tgaccggcgg
     9841 cggctgcgcc gcaagctgac gctctggcgc gtgctggcct gtgctgccgg tgctgcagcg
     9901 ctcattgcgc tcgggttgat gtcaggcggc aaggaactgg tcgacagcca gtccgcacat
     9961 atcgcccggc tcaccgtgag cggcctgatc acatcggaca agccgatgct ggaggcgatc
    10021 cgcaaggtca aggatgcagc ctccgccaag gcggcgatcg tgtcgatcaa cagtcccggc
    10081 ggcacgacga cgggttcgga agcgctgttc cgtgaattgc gcgatcttgc cgtagccaag
    10141 ccgaccgtcg ccgtggtcac cggtacggca gcctccggcg cctatgtcgc ggccatcggc
    10201 accgaacgca tcttcgcgcc ggagactgcg ctgatcggct cgatcggcgt gattgtgcaa
    10261 tatcccaatt tcgtcaagac gctggaactg atgggcgtga aggtggaagc ggtgcgctcc
    10321 tcgccgctca aggcgatgcc gagcggtgtg gagccgacct tgcccgaggc gcgggccgcg
    10381 ctggaagcct cggtggccga ttcctatgcc tggttcaaag ggctggtcaa ggatcggcgc
    10441 agcttgagcg aggaggaact ggtcaaggtt tccgacggac gggtatttac cggccggcag
    10501 agcgttccac tcaaactggt tgatcagatc ggctccgaga aggaggcgat tgcctggctg
    10561 gaaagcgaga aatcgattgc caagtcgctc aaggtcaggg actacaagcg cgttgatccc
    10621 aacgacaggc tggggctgtt ctcgcttgcc gccgggatcg ccgatttctt cggtcacgag
    10681 gagtttgccg cctcgttgcg cacgaccgga cgcggcatcg cggcgcagtc gcttgacggg
    10741 ctgttgtccg tctggcagcc tcgcatcgaa aactgataca aattcaaccg attacgccgt
    10801 gacaggagcg tatcaatgat caagtccgaa ctggtgctga agattgcgga acagaatccg
    10861 catctctacc agcgcgatgt cgagaacatg gtcaattcca tcctcgacga gattgtcgat
    10921 gcgctcaagc gcggcgaccg ggtggagttg cgcggattcg gcgccttttc ggtcaagcgc
    10981 cgcgacgccc gtgtcggccg caatccgcga accggcagca aggttgccgt ggcgcagaaa
    11041 gtggtgccgt atttcaagag cggcaaggaa atgcgccagc ggctgaacaa gcagccagcc
    11101 cgggcgaaaa agagctaggg catgctgcga aaaagtggca acgggttttc gcggaaagca
    11161 tgcactaaac tattgtaatc gatcacgtta tctctcgttt ggatgattcc atcaaaccga
    11221 gggcgtgatc taggggtccg gcgggagaaa acgacgatgc tggctttctt gcgtctgctg
    11281 ctgctcgcgc cgattgcagt ggtgctgatc atgctggcgc tcgccaaccg gacgccggtg
    11341 ccgctgtcat tcgatattgt cgggctggaa gcacaatcgg tgacggtgcc gctctatgtc
    11401 gcgctgctgg gggcggttat gcttggtatc ctgattggcg gcatcgcctg ctggatcggc
    11461 cagggcaaac accgcaaggc agcgcgtgag gcaggccgtg cagccgatca ggcccgcgcc
    11521 gaagccgagc ggctgaagct cgcggcctcg ccgctcgcgg cattgccgcc gcgctgacag
    11581 catcggttct cagacatgcc gctgagcgtc aagatctgcg ggctgtcgac gcccgcaacg
    11641 gtcgaggccg cccttggcgc cggcgccgac atgatcggct ttgtctttca cccgaaaagc
    11701 ccgcggtttg tcagccctga tgctgccgcg cgcctcgcca gggccgcccg tggacgggtg
    11761 gaaatcgtgg cactgaccgt caacatgacg ccggaagccg ccgaagatct cgccaatgtt
    11821 ctcaagcctg actggttcca gttgcatggc gatgaggcgc ccggtctggc cgaggccacc
    11881 cggcaggcaa ccggctgccg tatcctgcgg gcgatcggcg tggagacggt ggccgatgtg
    11941 acgcgcgccg gcatccatgc gctggccgct gaccggctgc tgatcgacgc caaggcgcca
    12001 aaggatgctg cctatccggg cggccatggc cggccgttcg actggaacat tctggcagcg
    12061 cttgaccctg gcctgccgtt catgctgtcc ggcggattga cgcccgagac ggttggcgcc
    12121 gcccttgcca cagtgcgcgg gcttggtgtc ggcatcatcg gggtcgatgt gtcgtcgggt
    12181 gtcgagacgg cgcctggcgt caaggatgcc gggaagatcc acgcctttat tgccgcttgt
    12241 cgcgcggcgg gataaggaag gcgcctcgtc gcgtccgccg acccaaaggt tcagtcgcgc
    12301 cgtattggat tgaggacaca cgatgaacgc gcccgataaa ctcaattcct tccgatccgg
    12361 cccggatgag cgcggctttt tcggcatttt cggcgggcgt ttcgttgccg agacgctgat
    12421 gccgctggta ctcgatctcg agcgcgccta tggcgaggcc cgcaacgatc cggctttccg
    12481 cgaagaaatg gtgcgcatgg gccgtgatta tatcggccgg ccctcgccct tgtattttgc
    12541 tgaacggttc acccgccatt tcggcggcgc caaggtttat ttcaagcgcg aggaactcaa
    12601 ccacaccggc gcgcacaagg tgaacaacgt gcttggccag atcctcctcg ccctacgcat
    12661 gggcaagacg cggatcatcg ccgagaccgg cgccggccag cacggcgtcg ccactgccac
    12721 gctctgcgcc cgctacgggc tcaaatgcgt ggtctacatg ggcgccgttg acgttgagcg
    12781 gcagaagccg aacgtgttcc gcatgcgcat gctcggcgcc gaggtggttc cggtgcaatc
    12841 cggcgccaag accctcaagg acgcgatgaa cgaggccttg cgcgactggg tgaccaacgt
    12901 gcatgacacg ttctattgca tcggcacggt cgccggcccg catccctatc cggcgatggt
    12961 gcgcgatttc cagtcggtga tcggcatcga aacacgggca cagatgctgg atgccgaggg
    13021 ccgtttgccg gattcgctgg tggcggcggt gggcggtggc tccaacgcca tcggtctgtt
    13081 ccaccccttc cttgatgatc cgtcggtgga gatctacggc gtggaggcgg caggccatgg
    13141 cattgccagc ggcgcccatg ccgcctcgat ggccggcggg cggcccggcg tgctgcacgg
    13201 caaccgcacc tatctcctga tggacgacga cgggcagatc aaggatgccc attcgatctc
    13261 ggcggggctc gactatcccg gtgttgggcc ggaacattcg ttcctccacg acgtcaaacg
    13321 ggtgcgatac gaaccgatca ccgatgatga ggcgctggcc gccttccaga cctgctcgaa
    13381 gcttgagggc attatacccg cgctggaatc ggcccatgct ctggcgctgg ccgagaagct
    13441 ggcgccacag aaacccaagg accatctgat ggtgatcaac gtctccggcc gcggcgacaa
    13501 ggacattttc accgtcgccg agcatctggg gggcatgtga tgaccaagtc gcgcattgcc
    13561 gaccgctttg ccgcctgcaa ggccgagaac cgcgccgcgc tggtcacctt caccatggcc
    13621 ggcgatccgg attttgccac cgcttcggca atcctggccg ggctgccggc cgcaggcgcc
    13681 gatgtgatcg aactcggcat gccgttttcc gatccgatgg ccgatggccc ggcgatccag
    13741 cttgccgggc agcgggcgct ggctgccggg caaacactgg caaagacgct tggaatggtg
    13801 cggagcttcc gcgcaagcga cccggcaacg ccgatcgtgc tgatgggtta ctacaacccc
    13861 atctacatct acggcgtcgg gcggttcctg aaggaggctg tcgcggccgg cgtcgatggt
    13921 ttgattgtcg tcgatctgcc gccggaaatg gatgatgaac tctgcctgcc ggcgctgaag
    13981 gcgggccttc atttcatccg tctggcaacg ccgaccaccg acgacaagcg cctgcccacg
    14041 gtgctcaaga acacctcggg atttgtctat tatgtctcga ttaccgggat taccggcgcg
    14101 gcgaccccgg atttctccaa ggtggcgaac gcagtggcgc ggatcaagca gcatacgccg
    14161 ctgccggtcg cagtgggttt cggggtgaag acgcccgaac atgccatggc cattgcgggt
    14221 ggcgccgatg gggtggtggt tggctcagcg ctggtcgatg ccgtgcgcac ttcgctcagc
    14281 gacggcaagg caagcgccgg gacagtggcc gccgtcaccg atctggtcag ctcgctggcc
    14341 gccgcggtgc gtgcgacccg acgcgacctt tgagtttgcc ggtgaattgg gctagaccac
    14401 ccgcaatcca attgcgagag catccatgaa ctggatcacc aatgtcgtcc gccccaggat
    14461 caacagcctt ctcaagcgcg agacgccgga gaacctgtgg atcaaatgcc ccgataccgg
    14521 gcagatggtg tttcacaagg atgtcgaggc caatctgttc gtcatcccgg gttcggagca
    14581 ccatctcaag atgggagcca gggcgcggct ggccagcatg ttcgatggcg gggatttcga
    14641 ggagatcgcc gtgcgggaga tgccggtcga tcctttgaag ttccgcggca aggaccgcta
    14701 catcgacaag ctcaaggaag ccaaggcggc aaccggcctc gccgatgcgg tcaagatcgg
    14761 ctacggtcag gtcgacggct tgccggtcac catcggcgcg catgatatgg acttcatcgc
    14821 cggctcgctc ggcatggcgg cgggcgaggc ggtcatcacc gggctggaaa cggcagcgcg
    14881 gcgtggcaca ccgttcgttc tgttcaccgc ctcgggcggc gcgcgcatgc aggaaggtat
    14941 cctgtccctg atgcagatgc cgcgaaccac ggttgcggtg cggcgtctgc gcgcggcgaa
    15001 gaagccctat atcgtcgtgc tgacccatcc gacaaccggc ggcgttaccg cttcctatgc
    15061 gatgctgggc gatgtgcatc tggccgaacc cggtgcgctc atcggctttg ccggcccgcg
    15121 tgtcatcgag cagacggttc gcgagaaact gcccgatggg ttccagcggg cggaatatct
    15181 caaggatcat ggcatggttg acatggtcgt gcatcgcaag gacatgaagg tgaccatcag
    15241 ccggctctgc cgcgtgctga tgaaggcgcc agctgccgcc tgaactgttg gcctgtgatg
    15301 aaactctcgg aagtctatct gcagcggttc caggcgctgc acccggtgtt gatcgacctg
    15361 tcgctggggc gcattgaaag acttctggcc aggctcggcc atcccgagcg gcgcatgggg
    15421 ccggtcattc acgttggcgg aaccaatggc aagggttcga ccattgcctt cctgcgcgcc
    15481 atgctggaag cggcaggcaa gcgggtgcat gtgttcacct cgccgcatct ggtcgccttc
    15541 aacgagcgca tcagactggc cgcaccgggg ggcgggcaac tggcgacgga ggagcagctc
    15601 gtcgccgcct tcgagcatgt tgaagcggtg aacggggccg atccgatcac gtttttcgaa
    15661 atcaccaccg cagcggcctt ccacctgttt gccacccatc ctgccgatat cacgctggtc
    15721 gaggtgggcc ttggcggcgc ctttgatgcc accaacgtgt tcgagcagcc ggccgctgcg
    15781 gtcatcacac cggtgtcgct ggatcacaag gagcatctgg gcgatacggt ggagaagatc
    15841 gccaccacca aatccggcat cttcaagcgg ggtgcgccgg ccatcatcgc cccgcaggac
    15901 gagacgccaa gccgtgtgct gcaacgtgcc gccgaacgca tcggcgcccg ccccatggtg
    15961 ggcggcgagg attttgccct gcatgaagag catggccgcc tggtctatca ggacgccgaa
    16021 ggattgatcg acctgccgct gccacgcctg ccgggccgcc accagcatat caatgctgcc
    16081 acagccattg cgacgctgcg aaccgtgttc ggtgccggat ttccggtggc ggccatcgaa
    16141 aaggggctgc agcaggtcga atggccggcg cggctgcaac acctcaccgg acagcttgcc
    16201 gcgttgacgc cgtcaggcgc cgaactctgg cttgatggcg gacacaatgc cgacggcgcg
    16261 cgcgcattgg ccgccgccat ggccgacctc gaggagcgca acccgaggcc gctcgtggtc
    16321 gtcctcggat tgatggcgcg caaggatgcg gacgccatac tggagccgtt ccgcggtctg
    16381 gcgcaggaat tctatgcggt cgacattccc ggcaacgaaa agaccgcgcg caaggccgat
    16441 gatctcgcaa tcctggcccg cgatctcggc ttgccctgcg cagtggccgg cgatgtcgcg
    16501 cagacattgc gctttctcag ccagcggtcc tggccgcgcc caccccgcat cctcattgcc
    16561 ggatcgctct atctggcggg tgaggtcctg aaagccgacg gcagcctgcc aaaatagcga
    16621 tggcccggtg caggcaccgg gccattgcag cgttgacggt tcgaccggtc agatcgacgc
    16681 ctgaatccag cgctggagat caccctttgc tgcggcgccg accttggtgg ccgccagttt
    16741 gccgcccttg aacaccatca gggtcgggat cgaccggatg ccgagcttgg cggcaacatt
    16801 ggggttctcg tcgatattga ccttgaccac cttgaccttg ccggccatct cgacggcgat
    16861 ctgatcaagc gaaggcgcga tcatgcggca ggggccgcac cattccgccc agaaatccac
    16921 caccaccggt tcggcggatt tcagcacgtc ggcttcaaaa ctggcatcgg tcacttttac
    16981 cgcgttgctc atggcagatc ctcgggttgc ggaatcatga aacgttggtc ttgtctctag
    17041 ctaggaatgc gggcgtcgac ggtcaagcga catcacaaca gggtgatcgc gtcgtccagg
    17101 catttggcgt cgatccggtc aatgcgcccc tgctgtatcc aaacgagcgc cgcctcgatc
    17161 cggcggtcgg gatagagttg tgccagaagt gcggcataga gcgccagctg aacgatatgt
    17221 ccaggcattg ccgcgcctgc aggcggtgga cgcccggtct tgaaatcgac gacggtgacg
    17281 cgatcgggct cgaccagcag ccggtcaatc cgtccggcaa cccggagcct gcgcccgtca
    17341 gggtgtggaa tggcaccggc aacggccact tcggcctcgg cggcagggcc gaacaggcgc
    17401 tgacccagcg gctcgttcaa taaagccagc accggttcga gcaagcctgc gcgcgcttgg
    17461 gtcgacaggc gcggcgcacg gtccgccagc cagcttgccg cagcctc
//
LOCUS       DAOMXG010000033        17295 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_1880_length_17295_cov_9.166183, whole genome shotgun sequence.
ACCESSION   DAOMXG010000033 DAOMXG010000000
VERSION     DAOMXG010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 17295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17295
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1880_length_17295_cov_9.166183"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            239..1801
                     /locus_tag="PLQ11_03845"
     CDS             239..1801
                     /locus_tag="PLQ11_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HRK24068.1"
                     /translation="MTDQTRPMHRLDRRQALATGLAALACAGTGSAAQEAALPGAELF
                     ADATPFDRDRLVDHARALAKRPYAAPNAPLPDGLANLTREQYATIHARPERLVWSGEN
                     PGLVLEPLHRGFVYSAPVHVSVIENGVTRKLVYSPARYDFGRLQAPQANVGDIGFSGV
                     RILGLSTDLPSPREIVTFQGASFLRAVARGQTPGTMARALALRTGEPKGEEIPFFRAL
                     WIEKPQRGDLITVHALLDSESVCGVFRYTIRIGDVTLIDTEATLFPRVALDNFGLAGM
                     QAGHLYGWLDRRNVDDIRPVVYEVAGVQMHTGNGEWVWRPVTNPKQLQISAFSADNPR
                     GFGFVMRDRDFTSFQDHENRYDLRPTLWVEPIGDWQAGVVQLVEIPSDNEGHDNIIAQ
                     WRPREPLAAGAEYSYAYRQYWSWLPPVRPNLAMTIGTRIGRGAQRRRKFVIDFAGERA
                     QSLRPGDIQAAFWCSNNGMSNLRILSVNEQRPLRVTFELDPGNETLIELRLVLMQANE
                     PVSETWLYRWTQ"
     gene            1789..3942
                     /gene="mdoH"
                     /locus_tag="PLQ11_03850"
     CDS             1789..3942
                     /gene="mdoH"
                     /locus_tag="PLQ11_03850"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016917967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HRK24069.1"
                     /translation="MDPVTIPLARPGDAPAMPAESPLAMPTQTFARFSREERRKPLLA
                     ASLASPVWIRLWVFGGGLALTAYGGWEMHNVINVSGTTALQWLLLVLFLANFVWIALA
                     FTGGVAGFVSLLMQRPAGLPPVTALATRTAVVMPIYNEAPSRVFAAMQTMAEGVSATG
                     LGSQFDFFFLSDTTDPDAWIGEERAYFALREKFPADIRLFYRHRARNTGRKAGNIADF
                     VSRWGGAYEHMLVLDADSLVEGETIVRLAGAMEADPDSGIIQTLPLIINRNTLFARLQ
                     QFAARVYGPIIAEGLRLWMGRDGNYWGHNAIIRTRAFADHCGMPELKGKPPLGGHILS
                     HDFVEAALIRRAGWNVTMVPGLVGSYEESPPSLIDLAGRDRRWAQGNLQHTRIVGAAG
                     FRWATRHHFLTGIMSYLASPLWLAQLVVGILLVLQAHFIRPEYFTGEFSLFPAWPRFD
                     AERALNLFILTMAILLAPKLFGVIVALTRSEERRAYGGTIRLVISSLVEIIMSALIAP
                     IMMLIQSGAVFQILFGGDTGWKPQRRDDGSVPISDIVRRHRGHVALGIFTGIVTYLIS
                     PSLFAWMSPTILGLLLAILISWGTGLVSVGIALRRFGLLWTPEERSPPEIAERANGLQ
                     AHYGDFDQIDGIRALHGDARLREHHTALLPHLPRRRRGEIDRDHVVAEAKIADAETIA
                     DASHWLTAREKTIAFSDRALVAMLAGLHKGKEHPK"
     gene            4002..4439
                     /gene="lysM"
                     /locus_tag="PLQ11_03855"
     CDS             4002..4439
                     /gene="lysM"
                     /locus_tag="PLQ11_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HRK24070.1"
                     /translation="MGLFSFVKSVGEKLFGASEAKAATADELKKEVAKHGLNAEGLDI
                     AVSGDTVKVSGTAMSQADAEKIILALGNTIGVAQVDSSLIAKQAGPAARMYTVKKGDN
                     LSKIAEAMYGKGHANDYGKIFEANKPMLKHPDKIYPGQVLRIP"
     gene            4636..6123
                     /locus_tag="PLQ11_03860"
     CDS             4636..6123
                     /locus_tag="PLQ11_03860"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HRK24071.1"
                     /translation="MALLASIAPPAAPEETLRLAPHNIEAEQALLGAIMVHNEAYDRV
                     SDFLRPDHFYEEIHARIFEVAGKLIHAGKLASAITMRTFLPDQDLGGLTVAQYLVRLT
                     QGATTIINAGEYGRVIYDLALRRNLIVIGEDMVNLAFDAPVEANPRSQIEDAERKLFA
                     LAESGRYEGGFQSFSQALVTAMDVAAKAYERDGHLSGIATGLTDLDRQMGGLQKSDLI
                     VLAGRPGMGKTALATNIAFNIARAWRGQRAANGDMETVNGGIVGFFSLEMSAEQLATR
                     IIAEQSGIPSYKIRRGDIDERDFEALTDAVRTMQEIPLYIDQTGGISIAQLTARARRL
                     KRQHGLDMIVVDYIQLLSGAAKKGDNRVQEVTEITTGLKALAKELDVPIIALSQLSRQ
                     VEARDDKRPQLSDLRESGSIEQDADVVMFVFREEYYLKNREPKPGGDDHLKWQTEMEK
                     IHGRAEVIIGKQRHGPTGTVPLHFDANITRFSNLAVEDALPQRFE"
     gene            complement(6268..7968)
                     /locus_tag="PLQ11_03865"
     CDS             complement(6268..7968)
                     /locus_tag="PLQ11_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HRK24072.1"
                     /translation="MSAAGSLDAVKVPRRAFDEARVATILVAVLVAILIVIIALPLWL
                     LLSKSFQDNTGAFVGLANFLTYFSTPSLVAALYNSLWVSLLTTVLVVPLAFVFAFALT
                     RSCMPFKGLFMALALLPIFAPSLLAAISMIYLFGNQGIMKGLLFGHSIYGPIGIIISE
                     VVYCFPHAVMIIVTALRLADGRLYEVADALGTSRTRVFFTVTLPGARYGLISAAFVVF
                     TLVATDFGIPKVIGGQFSVLATDAYKQIVGQQNFEMGAVVGLILLAPAVLAFFVDRLV
                     QKRQVALLSARAVPYEPKPSELRDGLLMAFCLVVGLILLGMLAMAVWASFITYWPYNL
                     TLSLKNYQFADYEPAGWGSYFASLKMAGLVAVIGTALVFFGAYLVEKTRVAPPLRAAA
                     HFLAMLPMAVPGLVLGLGYVFFVNAKWNPLNVFYATLTVLVVNTIAHFYTVAHITATT
                     ALKQIDNEFESVSSSLKVPFWTTFRRVTLPISLPAVLDIAVYMFVNALTTISAVIFLY
                     GPTTKLASIAIVHMDESGATAAAAAMAVCIVLTAIAAKLLHVLLDTMVFSRLQAWRRR
                     "
     gene            complement(7965..8315)
                     /locus_tag="PLQ11_03870"
     CDS             complement(7965..8315)
                     /locus_tag="PLQ11_03870"
                     /inference="COORDINATES: protein motif:HMM:NF019998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HRK24073.1"
                     /translation="MRSKDRVEIDGKAFTCSVGEGFQPGQNVLVGLRPETIRVRGVTG
                     KEANALKVRIDDLEFLGAFCRAELVEEGLQALHLIADFSANLIGDLGLAQGGSLFVEL
                     PADALMIFPGRSQA"
     gene            complement(8623..9195)
                     /locus_tag="PLQ11_03875"
                     /pseudo
     CDS             complement(8623..9195)
                     /locus_tag="PLQ11_03875"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(9156..10196)
                     /locus_tag="PLQ11_03880"
     CDS             complement(9156..10196)
                     /locus_tag="PLQ11_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018098758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_function: GO:0033226 - 2-aminoethylphosphonate binding
                     [Evidence IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HRK24074.1"
                     /translation="MRSKLKQTSLAMSAAVGLALALSGGALAQTKTKVTLYTALENEQ
                     LPIYKAQIEAAVPEADVVWVRDSTGVITARVLAEKDNPRADIILGLAATSLLAFEKLN
                     MLEEYKPKGSETLKANFRDNAAPFTWTGMDAFLAVACFNTAEAQKDKVATPQSWNDIL
                     KPEFKGKVVMPHPASSGTGYLMVAGWLQMFGEEKGWAFLDKLHDNIAVYTHSGSAPCV
                     QAAKGERLLGIGFDMRGAVEKSKGAPIDLILPTEGAGWDMEASAIVKGTKNLDVSKKV
                     MDWVASKGANELYAKYYALVAHPEVKNTPPNYPGDAEAKMAKNDFVWMAEQRERILKE
                     WSKRYESKAAPK"
     gene            complement(10256..11503)
                     /locus_tag="PLQ11_03885"
     CDS             complement(10256..11503)
                     /locus_tag="PLQ11_03885"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRK24075.1"
                     /translation="MFQAAEIVIIGAGAIGCSIAMHLAEGGAKDVLVIDKAGITHGST
                     WHAAGLVGQYRSAEDLARLMNISVRLFETMQTETPIDWKPVGSLRVAASVARMRELAA
                     AEPIARSYGIDLALLTPGEARGLYPYLNADDLHGAAFLPGDGYVDPSSLTQALAGKAR
                     RGGVRFVENTPVGALAHKNGRCTGVETAAGRIDCEKLVITGGVWARAIGRMLGLNIPV
                     AAVAHQYAVTGKRTDMPPNLPVLRDPDADFYVKPEVGGFAIGGWEAEPVLSAEGEMPS
                     TFGMELLPDDLERMLPVMEKAFHRIPVVGDVGIRRIVNGPIPVSPDGEPMLGRATGFS
                     NVYLGCGFTSGIAACGGAGQQLARLIQGGAFDFPIPSLDPARFGPATADFNEMNRQAC
                     RAYAGYYKLSASNSELAQASPSL"
     gene            complement(11507..12775)
                     /locus_tag="PLQ11_03890"
     CDS             complement(11507..12775)
                     /locus_tag="PLQ11_03890"
                     /inference="COORDINATES: protein motif:HMM:NF013044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HRK24076.1"
                     /translation="MSDLSNPFRYDRVAIASLASRYRSGGPLERAFYTAPEVFEADLD
                     RIWRRYWLYAGHSCTIPNAGDWFTYDVGPYSVIVVRGKDGEVRAFHNTCRHRGARVCN
                     AEHGHSRLIVCPYHAWSYELDGALRTRVKREFGRDETELGLHPVKLHDIEGLIFVALG
                     DDPVPFDQAVADIAPKMAHQGLKDAKLAHAARYVVEANWKLVFENNRECYHCATAHPE
                     YISGTYDSMRLNPAKLPEVERQTALADARFRAMGLDGAIASSAMTGRYWRATRAPLME
                     GFVTQTLDGKPASTLMGTFRQQDQRSAGTMRCTVFPNFWQHASDDHACAARLTPLDAA
                     RTEVVVTWYVHKDAVEGRDYTLGHLLPFWQRTAEQDWTICEANQKGVTSPAYTPGPYG
                     PKQEANLQDFIDWYLGALVPQRQHDRTAAE"
     gene            complement(12810..14258)
                     /gene="phnY"
                     /locus_tag="PLQ11_03895"
     CDS             complement(12810..14258)
                     /gene="phnY"
                     /locus_tag="PLQ11_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde dehydrogenase"
                     /protein_id="HRK24077.1"
                     /translation="MTAPGKLPFRKESMRIAGKLVATDDFIEVRNPYDNSVVGTVPAA
                     RPEHVREAFAKAAAFKPKLTRYERQQILLRTAELLHARREEIARLITAESGLCWKDSL
                     YEATRAYDVYSFAGQLAIKDDGETFSCDISPNGKARKIFTTRVPLMGVISAITPFNHP
                     LNMVSHKLAPAIATNNRVVLKPTELTPLTALLLADVLYEAGLPPEMLSVVTGNPSTMG
                     DSMITDPDADLITFTGSVRVGKYIAEKAGYRRIVLELGGNDPLIVMEDADLDKAAELA
                     VQGATKNSGQRCTAVKRILVVQSAAEEFSKLVVEKAKKIKCGDPMDPATDVGCVINAR
                     SAALFQARVDDAVAKGAKVLFGKRAEGALFHPTVVDHIPYECELVKEETFGPVIPIIR
                     VPDNIDDIVRISNSTAYGLSSGVCTNRLDYITRFIRDLDVGTVNVWEVPGYRIEMSPF
                     GGIKDSGLGYKEGVQEAMKSFTNVRTWSIPWG"
     gene            complement(14268..15515)
                     /gene="phnA"
                     /locus_tag="PLQ11_03900"
     CDS             complement(14268..15515)
                     /gene="phnA"
                     /locus_tag="PLQ11_03900"
                     /EC_number="3.11.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004434723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047400 - phosphonoacetate hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019636 - phosphonoacetate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetate hydrolase"
                     /protein_id="HRK24078.1"
                     /translation="MTARVEGAILVNGRSYHAPKAPTVVICIDGSEPGYIEKAIEAGR
                     APNFARFMQKGANLIAECVIPSFTNPNNLSIITGRPPHVHGIAGNFFYDAAAGREVMM
                     NDPSFMRAPTILAAMQEAGHKVAMVTAKDKLRLLLSNGLDYSTGSAIAFSSEKADKAT
                     KKDNGIDNVCAFVGMGVPDVYSAGLSEFVFAAGVKLVESFRPDLMYLSTTDYIQHKCA
                     PGSAIANDFYAMIDRYIGRLDELGCVVVATADHGMNDKHLKDGTPDVLYLEPLFDGWV
                     GKGKARVILPITDPYVVHHGALGSFATVHVPEGANIKDLIARLKATPGVERAMTRNAA
                     CNEFELPTDRLGDIVVISTRHKTLGTAPEKHDLSGLTEPLRSHGGLTEQRVPMISNRK
                     VSVPAGHRLRTFDVFAVALNHTS"
     gene            complement(15525..16667)
                     /locus_tag="PLQ11_03905"
     CDS             complement(15525..16667)
                     /locus_tag="PLQ11_03905"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HRK24079.1"
                     /translation="MPAIPNRAASETGDPLLLTPGPLTTSKSVKEVMVHDWGSRDATF
                     LKINREVLARLPEIVNATDSFVTVPIQGSGTFAVEAMLTSFVPKKGRVLVLINGAYGH
                     RANRILGIAGRRTTVYETPEDTPPDLRRVEAILKRVKSITHIFAVHCETTSGVLNPVH
                     EIGALAKKYGKGYLIDSMSAFGALPLDANKAHADAIAASSNKCIEGVPGLGFVLCRKS
                     VLPGLKGNATTLVLDVHDQWANFEKTGQYRFTPPIHVIVSFHQALTEFFAEGGQKGRG
                     GRYAANGKVLIDGMRAMGFKTLLPDTLQAPIIVTFHMPKNPKFDFQRFYDALKDRGYV
                     IYPGKLTVADSFRIGCIGRLYEKDMRGALAAVREVVTEMGVALPVT"
     gene            16784..>17295
                     /locus_tag="PLQ11_03910"
     CDS             16784..>17295
                     /locus_tag="PLQ11_03910"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRK24080.1"
                     /translation="MNVTGLRAFHAVAEAGSFTRAAAATGLSQPTLSSQVRLIEQQHE
                     AGLFDRKGRGVTLTPLGRRLYDITTRLFAAEDEARALLHGARALQRGHLRVVADSATH
                     VMPLLSVLKRRHPGLTFALSIDNSTGVLQRLFDYGADVGVTARATSDPRLHSRLIRRD
                     RLVLFAPRSHP"
BASE COUNT         3164 a         5719 c         5293 g         3119 t
ORIGIN      
        1 acaacccgcg tcattgtccc ggtcagcgcc aaattttcgc ttaatggaat cgtcgaatta
       61 cggcggcatc ggcaattttc gcttcatttg aatgaaatgc gcccgatccc cccggaccgt
      121 ttgcggcgtc tcgtgatcga atcctgatcc gctcctgctt ttagcgcttc cggttgacgc
      181 gccttctgta ttagacacca accgcgcaca gaggcgcggg ttaaggtcgc tctctcccat
      241 gacggatcaa acaagaccga tgcacaggct ggatcggcgg caggccctgg ccacggggct
      301 ggcggccctt gcctgtgccg gaaccggttc ggcagctcag gaagcggcgc tgcccggcgc
      361 cgaactcttt gccgacgcaa ccccgtttga tcgcgacagg ctggtcgacc atgcccgcgc
      421 actggccaag cggccgtatg ccgcccccaa tgcccccctg cccgacggat tggccaatct
      481 gacgcgcgaa caatatgcca cgatccatgc gcggcccgag cggctggtct ggtccggtga
      541 aaatccgggg ctggttcttg aaccgctgca tcgcggcttt gtctattcgg cgccggtgca
      601 tgtctcggtc atcgagaacg gcgtcacccg caagctggtc tacagcccgg cacgctacga
      661 cttcggccgg cttcaggccc cgcaggccaa tgtcggcgac atcggctttt ccggcgtgcg
      721 cattctcggc ctgtcgaccg acctgccatc cccgcgcgag atcgtgactt tccagggcgc
      781 ctcgttcctg cgggcggtgg cgcgcggaca gacgccgggc acgatggccc gcgcgctggc
      841 gcttcggaca ggcgaaccca agggtgagga aatcccgttt ttccgcgccc tgtggatcga
      901 gaagccgcag cgcggcgatc tgatcaccgt ccacgccctg ctcgattcgg agagcgtctg
      961 cggcgtgttc cgctacacga tccgcatcgg cgatgtgacc ctgatcgata ccgaggcgac
     1021 cctgtttccg cgtgtggcgc tggacaattt cggcctggcc ggcatgcagg ccggccatct
     1081 ctatggctgg ctcgaccgcc ggaacgttga tgatattcgc cccgtggtct acgaagtcgc
     1141 cggagtgcag atgcacaccg gcaacggcga atgggtctgg cggccggtca ccaatcccaa
     1201 gcagttgcag atctccgcct tcagcgccga caatccacgt ggcttcggct ttgtcatgcg
     1261 agaccgagac ttcacgtcat tccaggatca tgagaaccgc tacgacctgc gcccgacgct
     1321 gtgggtggag ccgatcggtg actggcaggc cggtgttgtc caactggtgg aaattccctc
     1381 agacaacgaa gggcatgaca acatcatcgc ccaatggcgg cccagggagc cgctggccgc
     1441 aggcgccgaa tattcctacg cctatcggca atactggagc tggctgccgc cggtccgtcc
     1501 caatcttgcc atgacaatcg gcacccgcat cggccgcggc gcgcagcggc gacgcaagtt
     1561 cgtcatcgat tttgccggcg aacgggcgca atcgctgcgg cccggcgata ttcaggccgc
     1621 attctggtgt tccaataacg gcatgagcaa cctgcgtatc ctgtcggtga acgagcagcg
     1681 ccccctgcgc gtcaccttcg aactcgaccc cggcaacgag acgctgatcg agttgcggtt
     1741 ggtgctgatg caggccaatg aaccggtgag cgaaacctgg ctataccgat ggacccagtg
     1801 acaataccgc tcgcccggcc cggcgatgca ccggccatgc cggccgaatc gccgctggcg
     1861 atgccgaccc agacctttgc taggttttcc agggaggaac gccgcaagcc cctgctggcg
     1921 gccagcctcg cttcgcccgt ctggatccgc ctgtgggtgt tcggcggcgg tctggccctg
     1981 accgcctatg gcggctggga gatgcacaac gtcatcaacg tcagcggcac caccgcgttg
     2041 cagtggctgc tgctggtgtt gttccttgcc aattttgtct ggatcgcgct ggcgttcacc
     2101 ggcggcgtgg cgggctttgt ctcgctgctg atgcagcgcc ctgccgggtt gcctccggtg
     2161 accgctctgg ccacccgcac ggcggtggtc atgccgatct acaacgaggc gccaagccgg
     2221 gtgttcgccg cgatgcagac catggcggaa ggcgtgtcag ccaccgggtt gggcagtcag
     2281 ttcgacttct tctttctgtc cgacaccacc gatccggatg cctggatcgg cgaggagcgg
     2341 gcctatttcg ccctgcgcga gaaatttccg gccgacatac ggctgttcta tcgccatcgc
     2401 gccaggaaca ccgggcgcaa ggccggcaac atcgccgatt tcgtctcccg ctggggcggc
     2461 gcctatgaac acatgctggt gctcgatgcc gactctctcg tcgaggggga aacgattgtc
     2521 cgccttgcgg gcgcgatgga ggccgatcct gattccggca tcatccagac gctgccgctg
     2581 atcatcaacc gcaacacgct gtttgcccgg ctccagcagt tcgccgcccg cgtctacggg
     2641 ccgatcatcg ccgagggcct caggctgtgg atgggcaggg acggcaacta ctggggccac
     2701 aacgccatca tccgcacccg cgcctttgcc gatcattgcg gcatgccgga gctgaagggc
     2761 aagccgccgc tcggcggcca tatcctcagc cacgatttcg tcgaggcggc gctgatccgc
     2821 cgtgccggct ggaacgtcac catggttccc gggctggtcg gctcctacga ggagagcccg
     2881 ccctcgctga tcgaccttgc cggccgcgac cgtcgctggg cgcagggcaa cctgcaacat
     2941 acccgcattg tcggcgctgc cggcttccgc tgggcgaccc gccaccactt tctgaccggc
     3001 atcatgagct atcttgcctc gccgctgtgg ctggcccagc ttgttgtcgg catcctgctg
     3061 gttttgcagg cccatttcat ccgtccggaa tatttcaccg gcgaattctc gctgttcccg
     3121 gcctggccgc gcttcgatgc cgagcgtgcg ctgaacctgt tcatcctgac tatggcgatc
     3181 ctgttggcgc ccaagctgtt cggcgtcatc gtcgcgctga cccgctccga ggagcgccgc
     3241 gcctatggcg ggacgatccg gctggtgatc tcgtcgctgg tcgagatcat catgtcggcg
     3301 ctgattgccc cgatcatgat gctgatccag tcgggcgcgg tgttccagat cctgttcggc
     3361 ggcgataccg gctggaagcc gcagcgccgc gacgatggtt cggtgccgat cagcgacatt
     3421 gtccgccgcc atcgtggcca tgtcgcactg ggcatcttca ccggaattgt cacctacctg
     3481 atctcgccat cgctgtttgc ctggatgtcg ccgaccattc tcggcctgct tctggccatt
     3541 ctgatttcct ggggcacggg acttgtcagc gtgggcattg cgctgcgccg tttcggcctg
     3601 ctgtggacgc cggaggaacg ctctccgccc gagattgccg agcgtgccaa tggcctgcag
     3661 gcccattacg gcgatttcga ccagatcgac ggcattcgtg ccctgcatgg ggatgcgcgc
     3721 ctgcgcgaac accatacagc gctgctgcca catctgccgc gccgccgccg tggcgagatt
     3781 gaccgcgatc atgtcgtcgc cgaggccaag atcgccgatg ccgagaccat tgccgatgcc
     3841 agccactggc tgaccgccag ggaaaagacc atcgcatttt cggatcgggc gctggtggcg
     3901 atgctggccg ggttgcataa gggcaaggaa caccccaaat aggcagagtt gagcccttgc
     3961 gggcgcgatt cttgggccgt tgcgcccccg tggaggatga aatgggattg ttttcgtttg
     4021 tgaagagcgt cggcgagaag ctgttcggcg ccagcgaggc caaagcggcg accgctgacg
     4081 aactgaagaa ggaagtggcc aagcacggcc tcaacgccga ggggctcgac atcgcggtgt
     4141 ccggcgacac ggtgaaggtc tccggcaccg ccatgagcca ggccgacgcc gagaagatca
     4201 ttctggcgct gggcaatacc atcggtgtcg cccaggtcga ttccagcctg atcgccaagc
     4261 aggccggccc cgccgcccgt atgtatacgg tcaagaaggg cgacaacctg tcgaagatcg
     4321 ccgaggcgat gtatggcaag ggccacgcca acgattacgg caagatcttc gaggccaaca
     4381 agccgatgct caagcatccc gacaagatct atccgggcca ggtgctgcgc attccgtaag
     4441 cgccgccgca aagcagttcg aaaggggcgc cgggcggcgc ccctttttgc gttgctcctc
     4501 cgccgccaga caggctttcc cgggctgcaa cgacgccctc gcagacggcc agcctgtgga
     4561 ttactgttga cggtgattct ttccttctaa gccgccccta ccgggggtag caccaaggaa
     4621 agacggaacc gaatcatggc cctgctggca tccatcgccc cccctgccgc cccggaggaa
     4681 acgctccggc tggccccgca caacatcgag gcggaacagg cgctgctggg cgcgatcatg
     4741 gtgcataacg aggcctatga ccgggtgtcg gactttctgc gtccggacca tttctacgag
     4801 gaaatccacg cccgcatctt cgaggtggcc ggcaagctga tccacgccgg caagctggcc
     4861 agcgcgatca ccatgcgcac cttcctgccc gatcaggacc tcggcgggct gaccgtggcg
     4921 caatatctgg tgcgcctgac gcaaggggcg acgacgatca tcaacgccgg cgaatacggc
     4981 cgcgtcatct atgatctggc gttgcgccgc aatctcatcg tcattggcga ggatatggtc
     5041 aatctggcct tcgacgctcc ggtcgaggcc aatccgcgca gccagatcga ggatgccgaa
     5101 cgcaaattgt tcgccctggc cgaaagcggc cgctacgaag gcggattcca gtcgttctcg
     5161 caggcgctgg tcacggcgat ggatgtcgcc gccaaagcct atgaacgcga cgggcacctg
     5221 tcgggaattg ccaccggatt gaccgatctc gaccgccaga tgggcgggtt gcagaaatcc
     5281 gacctgatcg tgctcgccgg tcgccccggc atgggcaaga cagcgctggc caccaacatc
     5341 gccttcaaca tcgcccgcgc ctggcgcggc cagcgcgccg ccaacggcga catggagacg
     5401 gtcaacggcg ggattgtcgg cttcttcagc ctcgaaatga gcgccgagca actcgccaca
     5461 cgcatcattg ccgagcaatc cggcattccc tcctacaaga tccgccgcgg tgacatcgac
     5521 gaacgcgact tcgaggcgct gaccgatgcc gtgcgcacca tgcaggaaat cccgctctat
     5581 attgaccaga ccggcggcat ctcgatcgcc cagctgaccg cccgcgcccg ccgcctcaag
     5641 cgccagcacg ggctggacat gatcgtggtg gactatatcc agcttctctc gggcgccgcc
     5701 aagaagggcg acaaccgggt gcaggaagtt accgaaatca ccactggtct caaggctttg
     5761 gccaaggaac ttgatgtgcc gatcatcgcc ctgtcgcaat tgtcgcgtca ggttgaggca
     5821 cgcgacgaca agcggccgca actctccgat ctgcgcgaat ccggctcgat cgagcaggac
     5881 gccgacgttg tgatgttcgt ctttcgcgag gaatattacc tcaagaaccg cgaaccaaag
     5941 cccggcggcg atgaccatct gaagtggcag accgaaatgg aaaagatcca cggccgcgcc
     6001 gaagtcatca tcggcaagca gcgccatggt ccgacaggca cggtgccgct gcatttcgac
     6061 gccaatatca cgcgcttctc caacctcgcg gtggaggacg ccctgccgca gcggttcgaa
     6121 taggccgctt ggcctctcac ccctcacccc aaccctctcc ccgttcacgg ggagagggag
     6181 atgcgcgtga gtgtctttcc ctctccccgt gaacggggag agggtcaggg tgaggggcaa
     6241 tcggccgagg tagcggatcg aaccggccta ccgcctgcgc caggcctgca ggcggctgaa
     6301 caccatcgta tcgagcagca catgcagcag cttggcggca atcgccgtca gcacgatgca
     6361 gacggccatg gccgccgccg cagcggtggc gcccgattcg tccatatgca cgatggcgat
     6421 ggaggcaagc ttggtggtcg gcccgtagag aaagatcacc gccgagatcg tcgtcagcgc
     6481 attgacgaac atgtaaacgg caatatccag caccgccggc agcgagatcg gcagcgtcac
     6541 ccggcggaac gtcgtccaga acggcacctt cagcgatgag gagaccgatt caaactcatt
     6601 gtcgatctgc ttgagcgccg tcgtcgcggt gatatgcgcc acggtgtaga aatgcgcgat
     6661 cgtgttgacc accagcaccg tcagcgtcgc atagaacaca ttcagcgggt tccatttggc
     6721 attgacgaag aacacatagc ccagccccag caccagcccc ggaaccgcca tcggcagcat
     6781 ggcaaggaaa tgcgccgcag cgcgcagcgg cggcgccacc ctggttttct cgacaagata
     6841 ggcgccgaag aacaccagcg cggtgccgat caccgcaacc agcccggcca tcttcagcga
     6901 agcgaaatag gagccccagc ccgcaggctc ataatcggca aactgataat tcttcagcga
     6961 cagcgtgagg ttgtagggcc agtaggtgat gaacgaagcc cacaccgcca tcgccagcat
     7021 gcccagcagg atcaggccaa caaccagaca gaaggccatc agcagcccgt cccgcagttc
     7081 cgagggcttg ggctcatacg gcacggcgcg ggccgacagc agcgccacct gccgcttctg
     7141 caccagacga tcgacgaaga aggccagaac cgccggcgcc agcaggatca gcccgaccac
     7201 agcgcccatt tcgaaattct gctggccgac gatctgcttg taggcatcgg tcgccagcac
     7261 gctgaactgt ccgccgatca ccttgggaat gccgaaatcg gtggcaacca gcgtgaacac
     7321 cacgaaagcc gcgctaatca gcccgtagcg ggcccccggc agcgtcaccg tgaagaacac
     7381 ccgggtccgg ctggtgccga gggcgtcggc cacttcgtag agccgcccgt ccgccagccg
     7441 caacgccgtg acgatgatca tcaccgcatg cgggaagcaa tagacaacct ccgagatgat
     7501 gatgccgatc ggcccgtaga tcgagtgacc gaacagcagc cccttcatga tcccctgatt
     7561 gccgaacagg tagatcatcg aaatcgccgc cagcagcgac ggcgcgaaga tcggcagcag
     7621 cgccagcgcc atgaacaggc ccttgaacgg catgcatgag cgcgtcagcg caaaggcgaa
     7681 cacgaaggcc agcggcacca ccagaacggt cgtcagcagc gacacccaca ggctgttgta
     7741 aagcgcggcc accagcgatg gtgtggaaaa atacgtcagg aaattggcaa ggccgacgaa
     7801 ggccccggtg ttgtcctgga agctcttcga cagcagcagc cacagcggca gggcgatgat
     7861 cacgatcagg atggccacca gaacggccac cagaatggtc gcgacacgcg cctcgtcgaa
     7921 ggcccggcgc ggaaccttca ccgcatcgag cgagccggcg gcgctcatgc ctggctccgc
     7981 ccggggaaga tcatcaatgc atcagccggc agttcgacga acagcgagcc gccctgcgca
     8041 aggccgagat cgccgataag attggcggaa aagtcggcga tcagatgcag cgcctgcaat
     8101 ccttcctcga ccagttcggc gcggcagaag gcgccgagaa actccagatc gtcgatgcgg
     8161 actttcagcg cgttggcttc cttgccggtc accccgcgca cgcggatcgt ctccggcctg
     8221 aggccgacca gcacattctg acccggctgg aacccctcgc ccaccgaaca ggtgaacgcc
     8281 ttgccgtcga tctccacccg atccttgctg cgcacgaccg caggcagaaa tattgatgcg
     8341 cccgacaaat tcggcgacga acgtggttct gggccgccgg tagatgtcct ccgggctgcc
     8401 gacctgctca atcacgccgt ggttcatcac cacgatgcgc tcggccatcg acaacgcctc
     8461 ttcctgatcg tgggtgacca tgatcgtcgt cacccccagg cggcgatgca gcgcgcggat
     8521 ctcctcgcgc aaccgcagcc gcacctgggc atccagcgcc gacagcggct catccagcag
     8581 cagcagcttg ggcgaggtcg ccagcgcacg ggcgagcgcg acacgctgct gctggccgcc
     8641 ggacaattgc accggatatt tggcgccctg ctctttcagg ccgaccagcg ccagcaattc
     8701 ggcgacgcgg gcggcaatct cggctttcgg ccgcttctgg ttgaccagac cataggcgac
     8761 attgtcggca atcgtcaggt tcggaaacag cgcataggac tggaacacga tgccgaagtc
     8821 gcgctcggac ggcggcgcat cggaaacgtc ccgcccgtcc atcacgatct ggcctgatgt
     8881 ctgtcgatcc agtccggcaa tcgcgcgcag caaggtcgtc ttgccgcagc cggacgggcc
     8941 cagaaagcag acgaaatcac cgcgcgcgat atcgagattg atctctttca gcgcccggaa
     9001 cgccccgaat tccttgccga cattgcgcac ggacaggaac gcctgctccc ccgcggcgtt
     9061 cgcgttggcc cctgattgtg tcacgtgtcc ccccagaagg actgaacata aaaggcccag
     9121 ccggaacgcc ggctgggcct cgattgcctt gcggcttact tcggcgccgc cttgctttcg
     9181 taacgcttcg accattcctt gaggatgcgc tcgcgctgct cggccatcca gacgaaatcg
     9241 ttcttggcca ttttggcttc ggcatcgccc ggatagttag gcggggtgtt cttcacctcg
     9301 ggatgcgcca cgagcgcgta atatttggca tagagctcgt tggcgccctt ggaagcaacc
     9361 caatccatca ccttcttgga cacatcgagg ttcttggtgc ccttgacgat ggccgaggct
     9421 tccatgtccc agccggcgcc ttcggtcggc aggatcaggt cgatcggcgc gcccttggac
     9481 ttctcgaccg ccccgcgcat gtcaaagccg atgccgagca gccgctcgcc cttggccgcc
     9541 tgcacgcaag gcgccgagcc cgaatgggtg tagacggcga tattgtcatg cagcttgtcg
     9601 aggaaggccc agcccttttc ctcgccgaac atctgcagcc agccggcaac catcagatag
     9661 ccggtgccgg acgaggccgg atgcggcatc accaccttgc ccttgaattc gggcttgagg
     9721 atgtcgttcc acgattgcgg cgtggcgacc ttgtccttct gcgcctccgc agtattgaag
     9781 caggccaccg cgaggaacgc gtccattccg gtccaggtga acggcgcggc attgtcgcgg
     9841 aaattggcct tgagcgtctc gctgcccttc ggcttgtatt cctccagcat gttgagcttc
     9901 tcgaacgcca gaaggctggt ggcggcgagg cccaggatga tatcggcgcg cggattgtcc
     9961 ttctcggcca gcacgcgggc ggtgatgacg ccggtggaat cacgcaccca gaccacgtcc
    10021 gcttccggca ccgccgcctc gatctgcgcc ttgtagatcg gcagctgctc gttctcgagc
    10081 gcggtataga gtgtgacctt ggtcttggtt tgcgccagcg caccgccaga tagcgccagc
    10141 gccagtccaa ccgccgccga catcgcaaga cttgtctgtt tcagtttcga acgcatgctt
    10201 ttctccctgt tccagtgtcc gaaagacttt gctttcgtaa ctcccatcca tgacgttaca
    10261 atgacggcga cgcctgcgcc agttccgaat ttgatgccga cagcttgtaa tatccggcat
    10321 aggcacggca ggcctgccgg ttcatttcat tgaaatccgc cgtcgccggg ccaaagcgtg
    10381 ccggatcgag gctcgggatc ggaaaatcga aagcgccccc ctggatcagc cgcgccagct
    10441 gctggcccgc gcctccacag gcagcgatgc ccgaggtgaa gccgcagccg agatagacgt
    10501 tcgaaaatcc cgtggccctg ccgagcatgg gctcgccatc cggcgagacg gggattggac
    10561 cgttgacgat gcgccggatc ccgacgtcgc ccaccaccgg aatgcgatga aaggcctttt
    10621 ccatcaccgg cagcatgcgc tccagatcgt ccggcagcag ttccatgccg aaggtactcg
    10681 gcatttcgcc ctcggcggac agcacgggtt ccgcttccca tccgccgatg gcaaagccgc
    10741 ccacttcagg cttgacgtag aagtccgcat ccggatcgcg cagcacaggc aggttcggcg
    10801 gcatatccgt gcgtttgccg gtgacggcat actgatgcgc cacggccgca accggaatgt
    10861 tgagccccag catccgcccg atcgcccgcg cccagacgcc gccggtgatc accagctttt
    10921 cgcaatcgat ccgccccgcc gccgtctcga cgccggtgca ccgcccgttc ttgtgcgcca
    10981 gagcgccgac cggcgtgttc tcgacgaagc ggacaccgcc ccgccgcgcc ttgccggcaa
    11041 gcgcctgtgt caggcttgac ggatcgacat atccgtcccc cggcaggaag gcggccccgt
    11101 gcagatcatc ggcattgaga tagggataga ggccgcgcgc ctcacccggt gtcagcaggg
    11161 ccaggtcgat gccgtaggac cgggcgatgg gctcggcggc agcaagttca cgcatacgcg
    11221 ccacgctggc cgccacccgc agcgagccga ccggtttcca gtcgatcggc gtttcggtct
    11281 gcatcgtttc aaagagccgc accgagatat tcatcagccg ggcgagatcc tcggcggaac
    11341 ggtactgacc gaccagcccc gccgcatgcc aagtcgagcc atgggtgatg ccggccttgt
    11401 cgatgaccag aacgtccttc gccccgccct cggccagatg catggcgatc gaacagccga
    11461 tggcgccggc cccgatgatg acgatttccg ctgcctggaa catgccctac tccgccgcag
    11521 tccgatcatg ctgcctctgc ggaaccagcg cgccgagata ccagtcaatg aaatcctgaa
    11581 ggttggcttc ctgcttgggg ccgtagggtc cgggggtata ggcgggtgag gtgacgccct
    11641 tctggttcgc ctcgcagatc gtccagtcct gttcagccgt ccgttgccag aatggcagca
    11701 ggtgccccag cgtatagtcc ctgccttcga ccgcatcctt gtggacatac catgtcacca
    11761 caacctcggt gcgcgcagca tcgagcggcg tgaggcgcgc cgcacaggca tggtcatccg
    11821 aagcgtgctg ccagaaattc ggaaagaccg tgcaacgcat cgttccggcg ctgcgctgat
    11881 cctgctggcg gaaggtgccc atcagcgtcg aagccggttt tccgtcgagc gtctgggtga
    11941 cgaagccttc catcagcgga gcccgcgtcg cacgccagta tcgaccggtc atcgccgagg
    12001 aggcgatcgc cccgtccagc cccatcgccc ggaagcgcgc atcggcaagc gccgtctgcc
    12061 gctcgacttc cggcagcttt gccgggttga gccgcatcga atcgtaggtg ccggagatgt
    12121 attcgggatg cgcggtggcg caatgatagc attcgcggtt gttctcaaac accagcttcc
    12181 agttggcttc gaccacatag cgcgccgcat gggccagctt ggcatccttc aatccctgat
    12241 gggccatctt cggcgcgatg tcggcaaccg cctgatcgaa cggcaccgga tcatcgccga
    12301 gcgcaacgaa gatcagccct tcgatatcgt gcagcttgac cgggtgaagc ccgagctcgg
    12361 tctcatcgcg gccaaactcg cgcttgacgc gcgtgcgcaa cgcaccgtcc agttcgtagc
    12421 tccaggcgtg gtaggggcag acgatcaggc gcgaatgccc gtgttcggcg ttgcaaaccc
    12481 gcgcgccgcg atgccggcag gtgttgtgga aggcacggac ctcgccgtcc ttgccgcgca
    12541 ccacaatgac cgaatacggg ccgacatcgt aggtgaacca gtcgcccgcg ttgggaatgg
    12601 tgcaggaatg gccggcatag agccagtagc gccgccagat gcggtcgagg tccgcctcga
    12661 acacctcggg cgcggtgtag aaggcgcgct ccaacggccc gccgctccgg taacgcgagg
    12721 caagcgaggc aatcgcaacg cgatcgtagc gaaacgggtt cgacagatca gacatcgaga
    12781 tttcctccct gctttggcgc ctctcgaccc tacccccagg gaatcgacca ggtgcgcacg
    12841 ttggtgaagc ttttcatcgc ctcctgcacg ccttccttgt agccgagacc ggaatccttg
    12901 atgcccccga acggcgacat ctcgatgcgg tatccgggca cttcccagac gttgaccgtg
    12961 ccgacatcga gatcacggat gaaccgggtg atatagtcaa gccggttggt acagacaccc
    13021 gaggacaggc cgtaagccgt cgaattggaa atccgcacga tgtcgtcgat attgtccggc
    13081 acgcggatga tcgggatcac cgggccgaag gtctcttcct tcaccagttc gcactcatag
    13141 gggatgtgat cgaccaccgt gggatggaac agcgcacctt ccgcgcgctt gccgaaaaga
    13201 accttggcgc ccttggcgac cgcatcatcg acgcgggcct ggaacagcgc cgccgaacgg
    13261 gcgttgatca cacagccgac atcggtggcc gggtccatcg gatcgccgca cttgatcttc
    13321 ttggccttct cgaccaccag ctttgagaat tcctcggccg cagactggac caccaggatg
    13381 cgcttgactg ccgtgcagcg ctggcctgaa ttcttggtcg cgccctgcac cgccagttcg
    13441 gccgccttgt cgagatcggc atcctccatg acgatcaggg gatcgttgcc gccgagttcg
    13501 agcacgatgc gccggtagcc ggccttctcg gcgatgtatt tgccgacgcg caccgagccg
    13561 gtgaaggtga tcagatccgc atccggatcg gtgatcatcg aatcgcccat ggtcgaggga
    13621 ttgccggtga cgaccgacag catctctggc ggcagccccg cctcgtagag cacatcggcg
    13681 agcagcagcg cagtcagcgg ggtcagttcg gtcggcttca gcaccacgcg gttgttggtg
    13741 gcaatcgccg gcgccagctt gtggctgacc atgttgagcg gatggttgaa cggcgtgatc
    13801 gccgaaatca cccccatcag cggcacgcgc gtcgtgaaga tcttgcgcgc cttgccgttg
    13861 ggcgagatat cgcaggaaaa cgtctcgcca tcgtccttga tcgccagttg cccggcgaag
    13921 gaatagacat cgtaggcgcg cgtcgcctcg tagagcgaat ccttccagca caggccggat
    13981 tcggcggtga tcagccgggc gatttcctcg cgccgtgcat gcagcagctc cgccgtcctc
    14041 agcaggatct gctggcgctc atagcgcgtc agcttcggct tgaacgccgc cgccttggcg
    14101 aaggcttcgc gcacatgctc gggccgcgcc gccggcaccg tgccgacgac gctgttgtcg
    14161 taggggttgc gcacctcgat gaaatcatcg gtggcaacga gcttgccggc gatgcgcatc
    14221 gattccttgc gaaagggaag ttttcccgga gcggtcatat cctggcctca gctggtatgg
    14281 ttgagcgcga cggcgaacac atcgaacgtg cgtaagcgat ggccggccgg cacgctcacc
    14341 ttgcggttcg agatcatcgg cacgcgctgt tcggtcagcc cgccatgcga gcgcaagggt
    14401 tcagtcagac ccgacagatc gtgcttttcc ggagcggtgc ccagcgtctt gtggcgggtg
    14461 gaaatcacca cgatatcgcc caaccggtcg gtcggcagtt cgaattcgtt gcaggcggcg
    14521 ttgcgggtca ttgcccgttc gacgccgggc gtcgccttca gcctggcaat caggtccttg
    14581 atgttggcac cctccggcac atgcacggtg gcgaacgaac cgagcgcgcc gtgatggacc
    14641 acatacggat cggtgatcgg caggatgacc cgcgccttgc ccttgccgac ccagccatcg
    14701 aacagcggtt ccagatagag cacgtcgggc gtgccgtcct tcagatgctt gtcgttcatg
    14761 ccgtggtcgg cggtcgccac cacgacgcag cccagttcat ccagccggcc gatgtatcgg
    14821 tcgatcatcg catagaaatc attggcgatg gccgagcccg gcgcgcattt gtgctggatg
    14881 tagtcggtgg tcgaaagata catcagatcc ggccggaagc tttcaaccag cttgacgccg
    14941 gcggcgaaca cgaattccga cagcccggcc gaatagacat ccggcacgcc catgccgacg
    15001 aaggcgcaga cattgtcgat gccgttgtcc ttcttcgtcg ccttgtcggc cttctcggac
    15061 gagaacgcga ttgccgagcc ggtcgaataa tccagcccgt tcgacagcag cagcctgagc
    15121 ttgtccttgg cggtcaccat cgccaccttg tgcccggcct cctgcatcgc cgccagaatg
    15181 gtcggcgccc gcatgaagga gggatcgttc atcatcactt ccctgcccgc cgcagcgtcg
    15241 tagaagaagt tgccggcgat gccgtgcaca tgcggcggcc gcccggtgat gatcgacagg
    15301 ttgttgggat tggtgaacga ggggatcacg cattcggcga tcagattggc gcctttctgc
    15361 atgaaccggg cgaagttggg cgcccgcccc gcctcgatcg ccttttcgat atagcccggt
    15421 tccgagccgt cgatgcagat gacaacggtc ggcgccttgg gagcgtggta gctgcggcca
    15481 ttgacgagaa tggcgccctc aactcttgcg gtcatgtcag ttcctcacgt caccggcagg
    15541 gcgacgccca tttctgttac cacctcgcgc acggcggcga gcgccccgcg catgtccttt
    15601 tcatagagcc ggccgatgca gccgatgcgg aacgaatccg ccaccgtcag cttgccggga
    15661 tagatcacat agccacgatc cttcagcgcg tcatagaagc gctggaaatc gaatttcggg
    15721 ttcttcggca tgtggaaggt gacgatgatc ggcgcctgca gggtgtcggg cagcagggtc
    15781 ttgaatccca tcgcccgcat gccatcgatc agcaccttgc cgttggcggc atagcgcccg
    15841 ccgcgcccct tctgcccgcc ctcggcgaag aactcggtca gcgcctggtg gaacgagacg
    15901 atcacatgga tcggcggggt gaagcggtat tgcccggtct tttcgaaatt ggcccactgg
    15961 tcatgcacgt cgagcaccag cgtcgtcgcg ttgcccttca ggcccggcag caccgacttg
    16021 cggcatagca cgaagcccag gcccggaaca ccctcgatgc acttgttgga cgaggcggca
    16081 atcgcatccg catgcgcctt gttggcatca agcggcagcg cgccgaatgc gctcatcgaa
    16141 tcgatcaggt aacccttgcc gtatttcttc gcgagcgcgc cgatctcatg caccgggttg
    16201 agcacgcccg aggtcgtctc gcaatgcacc gcgaagatat gggtgatgct cttgacccgc
    16261 ttgaggatcg cctcgaccct gcgcagatcc ggcggtgtat cctccggcgt ctcatagacc
    16321 gtggtccggc gcccggcgat gccgagaatg cggttggcgc ggtgcccgta ggcgccgttg
    16381 atcaacacca gcacgcggcc cttcttcggc acgaagctgg tcagcatcgc ctcgacggcg
    16441 aaggttcccg acccctggat cggcaccgtg acgaaactgt cggtggcgtt gacgatctcc
    16501 ggcaggcgcg ccagcacctc gcggttgatc ttgaggaagg tcgcatcgcg cgagccccag
    16561 tcatgcacca tcacctcctt gaccgacttc gaggtggtca agggtcccgg cgtcagcagc
    16621 aagggatcgc cggtttccga ggcggcgcgg ttgggaatgg caggcatgga aagctcccga
    16681 tgaaacgtca ccgcacagta ggagagcccc aacttattcg tcaaataatg tatttggata
    16741 gacgtcatag attttttcta tattttatcg gacagaggtt tcgatgaacg tcaccggatt
    16801 gcgcgccttt cacgctgttg ccgaggccgg cagcttcacc cgtgcggccg ccgccaccgg
    16861 gctgagccaa cccacgctgt caagccaggt ccgcctcatc gagcagcagc atgaggccgg
    16921 cctgttcgat cgcaaggggc gcggggtcac actgaccccg ctcggtcgtc gcctctatga
    16981 cattaccacc cgcctgtttg ctgccgagga tgaggcgcgc gccctgctgc acggcgcccg
    17041 cgcgctgcaa cgcggccatc tgcgcgtcgt cgccgacagt gccacccatg tcatgccact
    17101 gctctcggtc ctcaagcgcc gccatccggg cctgaccttc gccctcagca tcgacaattc
    17161 gaccggcgtg ctgcaacgcc tgttcgacta cggcgccgat gtgggcgtca ccgcccgcgc
    17221 cacctccgat ccgcgcctgc acagccggtt gatccggcgc gaccggctgg tgctgttcgc
    17281 tccccgcagc catcc
//
LOCUS       DAOMXG010000034        17038 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_1913_length_17038_cov_10.340635, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000034 DAOMXG010000000
VERSION     DAOMXG010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 17038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17038
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1913_length_17038_cov_10.340635"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..1357)
                     /locus_tag="PLQ11_03915"
     CDS             complement(140..1357)
                     /locus_tag="PLQ11_03915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HRK24081.1"
                     /translation="MARLKPDTLTLTGVLALLTAIGPMATDMYLPVLPVIRTALDATT
                     SQTQATLSGFLIGYALAQLFYGPISDRKGRRPAMLAGLALFTLGSILCALAQDIRFLV
                     AARVLQALGGAGTVVLARAMVRDLYEGPRAGRELARMGSIMAVVPAVVPILGAGFAAI
                     WGWRSIFWFMAIAGPALALIVAMGLPESLRAPLTTRFSLAAMLRDFGAIFRMPTFRTY
                     TLTGGLTFAGFMAFLSGGSFVLQGVYRISDSAFALGFALVVVGYIIGTSFTQRKVGSW
                     GIDKTIRCGTLVAAPAAAAMVLAVATGWGGALGVFIPMTVFQASMGLILPLSSAGAMM
                     PFPEKAGSASSLFSVLQMSLAATSGALVGIALDHTPLALPVMILVCALAALTVFARHR
                     PAGGQAPPASLAE"
     gene            complement(1380..2174)
                     /locus_tag="PLQ11_03920"
     CDS             complement(1380..2174)
                     /locus_tag="PLQ11_03920"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HRK24082.1"
                     /translation="MFDWPPSFFRKRPAKPRIERLEVLHLGRSYPVAIRRNATARRLI
                     LRVRADTGEAVLTLPRRTNMSQARDFLDRHGGWLAARMARLPEAVAFEPGCIVPFRGL
                     PHVIEHAGSARGGVECRPGVDGDPGRLIVAGDPRHLARRLTDFFKREARAELARASAA
                     YARQLNVRITRISIKDTRSRWGSCSATGALSFSWRLILAPPEVLDYLAAHEVAHRVEL
                     NHSPRYWAVVARLCPHWQSSESWLTRHGAGLHRFGASDAATVAPLP"
     gene            complement(2222..3514)
                     /gene="phaZ"
                     /locus_tag="PLQ11_03925"
     CDS             complement(2222..3514)
                     /gene="phaZ"
                     /locus_tag="PLQ11_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HRK24083.1"
                     /translation="MNHHMYYLYEAAHSIISPSRAFVDTVGMFYRNPLNPLSQTSFGR
                     NLAAACELYERLTRRYGKPAFGLHHTVIEGEEVPVTESIVWERPFCRLIRFVRGPHPA
                     GLKQSKLLIVAPMSGHYATLLRGTVEAFLPSHQVYITDWEDARMVPVTEGRFDLDDYI
                     DYVQDILRHLGPDVHVIAVCQPSVPVLAAVARMEMENDPHAPCSITLMGGPIDTRKSP
                     TEVNKLAEKRGTDWFRRNCIMRVPFPNPGFMREVYPGFLQLSGFMAMNFDRHVEAHAE
                     MFNHLVEGDGDSADKHRDFYDEYLAVCDLTAEFYLQTIDTVFVQHALPNGTMTHRGKP
                     VDTSAIRRTALMTVEGEKDDISGVGQTKATHDLCVNIPKHRQLYHLQEKVGHYGVFNG
                     SRFQRHIVPAMNAFMEQAEASFERPGRPRLINGGKKTG"
     gene            3702..5042
                     /locus_tag="PLQ11_03930"
     CDS             3702..5042
                     /locus_tag="PLQ11_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ActS/PrrB/RegB family redox-sensitive histidine
                     kinase"
                     /protein_id="HRK24084.1"
                     /translation="MGAETKTAAPVVADTAPDQILRQNTLVRLRWLAVAGQLVSVLAV
                     HFGFGFALPLAAALAVIAASVALNVGLMLGFSVNHRLSALATAAVLAFDIVQLAALLA
                     LTGGLSNPFSMLFLAPVMVSATVLAPRYTLLLGVLAAGLASLLAIWHWPLPWASDQPL
                     TLPLLYRAGLWAAVLISLAFIATYTFKVADEARKLATALAATELVLEREQHLSTVAGL
                     AAAAAHKLGTPLGTITLVAKELDLQVPAGHQLKDDITLIRQEAARCREILTNISALGR
                     DDGGPLTRMAISQLVEELVAPYRPSDVVIDVRLDGPQPEPTLRRNPELFYALGNLIEN
                     ALDFAKDRIIVRALWTSESISLSISDDGPGFSVDVIQRLGEPYVTTRRHRLTEAGLRR
                     QVGGGHGLGLFISKTLLERGGGRIRWSNLPAPGGAHTEVYWHRSDFGRKFDQRG"
     gene            5093..5629
                     /locus_tag="PLQ11_03935"
     CDS             5093..5629
                     /locus_tag="PLQ11_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ActR/PrrA/RegA family redox response regulator
                     transcription factor"
                     /protein_id="HRK24085.1"
                     /translation="MRSMGDMSLLIVDDDKPFLTRLAAAMSKRGYQVTMACGVSEGLR
                     AIMTAPPAFAVIDMRLGDGNGLDVISELKRMRPDARAIILTGYGNIATAVTAVKLGAF
                     DYLAKPADADEIDAALKALPYSRPELPEHPMSADRVRWEHIQRVYESCNRNVSETARQ
                     LSMHRRTLQRILAKRAPR"
     gene            complement(5664..6728)
                     /gene="paaK"
                     /locus_tag="PLQ11_03940"
     CDS             complement(5664..6728)
                     /gene="paaK"
                     /locus_tag="PLQ11_03940"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase/reductase subunit
                     PaaK"
                     /protein_id="HRK24086.1"
                     /translation="MSRFHPLSVKDIRRETADSVSLVFNLPEALKQTFSFTPGQYLTL
                     RTMLGGTECRRSYSICSGLQDGELRIAVKKVAGGVFSTFANEKLKPGAVLDVLPPEGR
                     FVADPARDRHLVFFAAGSGITPVLSIIRSALAGNTRTRISLFYGNRTTASIMFREALE
                     DLKDRYLGRLSVFHILSRESQDIDILNGRIDQAKVALLTKTMVPPAEVDAYFLCGPYS
                     MVEEGRAALTASGVDAKRIKAELFSTDGAPRVAPKPSAAHATGGVSTVDCQLDGITYH
                     IEVRPDERIVDSAHAQGVELPYSCKGGMCCTCRCKVTEGKVTMDANYSLEPWEMEAGF
                     VLACQSRPQTPVVKLDFDAS"
     gene            complement(6739..7248)
                     /gene="paaJ"
                     /locus_tag="PLQ11_03945"
     CDS             complement(6739..7248)
                     /gene="paaJ"
                     /locus_tag="PLQ11_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaJ"
                     /protein_id="HRK24087.1"
                     /translation="MAVPAPAPDDAASRARAAAARVTDPEVPVLTIADLGILHGVEID
                     EAGVVRVTILPTYSGCPAMGVIALGIETELARDGFDKVAVRLSQTPAWSTAMMSDAAR
                     EKLRAYGISPPERASGKRALFEASADVTCPRCGSAETERLSEFGSTACKALWRCRRCR
                     EPFDHFKCV"
     gene            complement(7250..8014)
                     /gene="paaC"
                     /locus_tag="PLQ11_03950"
     CDS             complement(7250..8014)
                     /gene="paaC"
                     /locus_tag="PLQ11_03950"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaC"
                     /protein_id="HRK24088.1"
                     /translation="MTHASPNLPAAWLMRLGDNSLILGHRMSEWVSCAPTLEEDIALS
                     NLGLDLIGQTRALYTRACELEAAGRTEDMLAYRRPEEEFRNLLLVEQPNGNFAETMAR
                     HLIFSAYMLPLYEALKGSSDADVAGIATRSANEMAYHLRHSAEWMIRLGDGTGESHAR
                     AQEALDDLWAYVPELFETDDLDRAVAASGFGVDPAGLRGAFDATISEVLKRATLKRPA
                     DRHGIRGGRKGVHSEHLGHMLATMQHLQRAYPDAVW"
     gene            complement(8025..8312)
                     /gene="paaB"
                     /locus_tag="PLQ11_03955"
     CDS             complement(8025..8312)
                     /gene="paaB"
                     /locus_tag="PLQ11_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit B"
                     /protein_id="HRK24089.1"
                     /translation="MTEKATPLWEVFIRPRNGLAHRHVGSLHAIDAEMALQNARDVYT
                     RRGETLSIWVVPSNAIVASDPSAKDVLFEPTASKIYRHPTFYEVPEEVGHM"
     gene            complement(8367..9350)
                     /gene="paaA"
                     /locus_tag="PLQ11_03960"
     CDS             complement(8367..9350)
                     /gene="paaA"
                     /locus_tag="PLQ11_03960"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit A"
                     /protein_id="HRK24090.1"
                     /translation="MYAQALNVKDLAPVKPEDPEKLAAFQARIDAEEKIEPNDWMPEQ
                     YRRTLIRQISQHAHSEVIGMQPEGNWVTRAPTLKRKAQLLAKIQDEGGHGLYLYAAAE
                     TLGVSREELYQQLLSGRAKYSSIFNYPTLNYADMGAIGWLVDGAAIMNQIALCRCSYG
                     PYARAMILICKEESFHQRQGYEIMVAMCRGSKEQRAMAQDALNRFWWPAMMMFGPHDS
                     DSPHSEVSMKWKIKRFSNDGLRQKFFDATVPQAQYLGLTIPDPKLKFNKATGHWDHSD
                     IDWDEFVRVINGDGPCNRQRMKHRRQVEADGAWVREALFAHAAKRAARKAA"
     gene            complement(9420..10325)
                     /locus_tag="PLQ11_03965"
     CDS             complement(9420..10325)
                     /locus_tag="PLQ11_03965"
                     /inference="COORDINATES: protein motif:HMM:NF019829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaX family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HRK24091.1"
                     /translation="MTVSMTEAMSQYSLLSRCLTSAPRGTLRIWSLAVSFLGDCIVPR
                     GGLVGLSTISDVLGACGIDAGAVRTALSRLASDGWVARVRSGRNSYYTLTPTALAESQ
                     AASRRIYAADPPQAPCGWQVMITAGLPPDGHRRLRETLLRQGAGTLDTHSFILPDHHP
                     LRSEPGALLLPVSRLADDQARLVVANAFDLAALARSYGNFLANHADLASHLAAGHTPA
                     DGLEAVALRVLMIHRFRRIVLRDPGLPACYLPADWPGFAARKLAHELRASLSPAAESW
                     LDERLIEPVGGHSDMLQNLLASADN"
     gene            complement(10472..12310)
                     /locus_tag="PLQ11_03970"
     CDS             complement(10472..12310)
                     /locus_tag="PLQ11_03970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRK24092.1"
                     /translation="MGVRLPVQLPLPTLPLPADIAFLVRFGFSAASLKPIAARARRIG
                     AAAAEVLVASGLTTETVYARLLARHLQCNEPTNLVPHPRVRHQDALRQGWFRATTADG
                     RPVLAIAAIGPVARLLGQGALAHHARHVVVLERASYHDVLKRHFGEAIAYAAAHSVPA
                     DESARGGLTNRQMAVLGIAVSALAAVAMQAFEATLLLAPMLLGVVFLVMAGLQAAAVG
                     AGLPIVDIRPEQNDENLPLYSVLVPLYREAEIIGDLVAALARLDYPAEKLQVLLIVEE
                     HDAQTQAALAGLSLPSHMQVFVAPAGAPHTKPRALNAALPFATGERVVIFDAEDRPEP
                     QQLRKAAAMFAALPQSTACLQARLAIDNADDSMLTRLFAFEYAALFDVTKAGTARLGL
                     PVPLGGTSNHFRTDVLRQLGGWDAWNVTEDADLGIRLAMHGYHVADLNATTFEEAPVR
                     LGAWLHQRSRWLKGWMQTAITHSRAPCTAFKALGPLSFMAAVAQSAGIILGALGAPVF
                     MALLARRFATPAPFFSGNWVHVLADGVMLGLVVAGLIALVLPLMIGMARRDLWRLAPW
                     LMLLPLYLMLISLAAYKALWELLHQPHRWNKTTHGVARRRISPPGD"
     gene            12456..13286
                     /locus_tag="PLQ11_03975"
     CDS             12456..13286
                     /locus_tag="PLQ11_03975"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRK24093.1"
                     /translation="MSSILLALVVNVAGTTPVTADDTVAIPNLWDSRRKFDPPNLGSQ
                     RQIRFITESDYPPFHFVTPSGQLSGFEVDLARGLCDLLKVSCSIQAVRWDGLKDALMT
                     GQGDAIIAAMRITPEARKTFGFTTAYFRSPARFVVQRADPLVEPTLEQLSGKRVAVMG
                     QSAHEAYMRTFFPDVGRMVTQNASEALAAVRAGEVAAAFVDGTVAAFWLNGEQSGNCC
                     RFLGGGFTEARFFGEGAGIAVRPDAQALRQALDWALHRFAAEGGYARLYLKYFPVSIY
                     "
     gene            13394..15094
                     /locus_tag="PLQ11_03980"
     CDS             13394..15094
                     /locus_tag="PLQ11_03980"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HRK24094.1"
                     /translation="MSAPELREAALTAAAWPFEEARKLVARIERTGQDSVIFETGYGP
                     SGLPHIGTFGEVARTTMVRRAFELLTDGRVKTRLIAFSDDMDGLRKVPDNIPNREMVA
                     GFLGRPLTEVPDPFGTHPSFGAHNNARLRAFLDTFGFDYEFKSSTECYRSGLFDATLL
                     LFLRRLDDVQKIMLPSLREERAATYSPFLPVHPETRVVMQVPTEARAEAGTIVWTDPD
                     SGKTYETPVTGGHCKLQWKPDWAMRWMALGVDYEMAGKDLIDSVKLSSAIVRRLGGSP
                     PEGFNYELFLDEGGQKISKSKGNGLTIDEWLTYASPESLALFMYQKPREAKRLHFDVI
                     PRCVDEYLSFLDAYPRQDVRNRLGNPVWHIHNGAPPAAETLGQDPSANAPRTAGLSFA
                     LLLNLVAVANAETKDVLWGFLQRYAPGVSAATHPRIDALVGYAIRYYADFVKPGKVYR
                     EADEVETAALSALARALAALPPDSTAERVQEVVYDVARPIPRFQDMKAKGATPERPGV
                     SNLWFNTLYQILLGEERGPRFGSFAAIYGLGNTVRLIERALSGALRKDHAAFLESRAG
                     "
     gene            complement(15126..15752)
                     /locus_tag="PLQ11_03985"
     CDS             complement(15126..15752)
                     /locus_tag="PLQ11_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRK24095.1"
                     /translation="MLTHQQIWTAVDALAEKFGFSASGLARRAGLDPTTFNKSKRLTP
                     DGRERWPSTESIAKILKATGAGIDDFVSLIRPQGQQAAGMRLPLIDSEKAALPGSFDD
                     TGRPSGPDWSDISLPAAADTQAFALELASGAAPYREGDVLVIVPRDALRRGDRVFIRT
                     AAGRVILGEMRRETAQGIDIIPFGGDAGMVTVTKSEAELVGRIRWASQ"
     gene            15897..16241
                     /locus_tag="PLQ11_03990"
     CDS             15897..16241
                     /locus_tag="PLQ11_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017485456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HRK24096.1"
                     /translation="MPLIYKIMPATMWRDAVSAGAFTGAPVDHADGFIHFSAADQVRA
                     TAAKHFAGHRDLVLVAVTAEALGSALKWEVSRGGALFPHLYAPLVITQDVLVEPLPLG
                     ADGLHVFPEAIP"
     gene            16238..>17038
                     /locus_tag="PLQ11_03995"
     CDS             16238..>17038
                     /locus_tag="PLQ11_03995"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015950843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HRK24097.1"
                     /translation="MKDFLFGLARPVLMGLDAEAAHRATIAALKVAPELPLPIDPDVL
                     HVSAFGLDFPNPVGLAAGFDKHAEVANALFGLGFGFVEVGAVTPRPQPGNPRPRAFRL
                     MSDEAVINRYGFNSDGLDVVAGRLQRLRPRGIVGVNLGANKDSADRAADYEVLVRRLA
                     GHCAFLTANVSSPNTPGLRSMQSRAPLDDLLRRVIDARDAASEGRSRTPVLVKIAPDV
                     TDEDLDDIVAVARERQVDGMVISNTTIARPASLRDQALAGEAGGLSGRP"
BASE COUNT         3098 a         5365 c         5481 g         3094 t
ORIGIN      
        1 aacgcgccgc cccgccgcag cggacagcga aaggcccgtt ccgccccgca atgtcggaat
       61 taccggatca atcacgccgc ggccggacaa gcgggccgtt gaaggaccga gtgacaagga
      121 ccgtgatcgg ctcaaccgtc tattcagcca atgatgccgg tggcgcctga ccgcctgccg
      181 gtcggtgccg ggcaaatacc gtcagggctg ccagcgcgca aaccaggatc atcaccggca
      241 gggcaagcgg ggtgtggtcc agcgcaatcc cgacaagcgc tcccgatgtg gcggcaaggc
      301 tcatctgcaa cacggagaac agcgaagagg cgctgccggc tttttcagga aacggcatca
      361 tcgctccggc gcttgatagc ggcaggatga ggcccatcga cgcctggaac acagtcatgg
      421 ggatgaacac gccgagtgcg ccgccccagc cggtggccac cgccagcacc atcgctgcgg
      481 cggccggagc cgcaaccagc gtgccgcagc ggatcgtctt gtcgattccc caactgccca
      541 ccttgcgctg cgtgaagctg gtgccgatga tgtacccgac cacaacgagg gcaaagccga
      601 gcgcgaacgc gctgtcggag atccggtaga cgccctgcag cacgaaggag ccgcccgaca
      661 aaaaagccat gaagccggca aaggtaagcc cgcccgtcag cgtgtaggtc cggaaggtcg
      721 gcatccggaa gatggcgcca aaatcacgca gcatggcggc aagcgaaaac cgggtagtca
      781 gcggcgccct gagcgattcc ggcaggccca tggcaacgat gagagccagc gccgggccgg
      841 cgatcgccat gaaccagaag atcgagcgcc agccccagat tgctgcaaat ccggcaccga
      901 ggatgggaac gactgccgga accaccgcca tgatcgagcc cattcgcgcc agttccctgc
      961 cggcgcgagg cccctcgtaa agatcgcgca ccatggcccg cgccagcacc accgtgccag
     1021 ccccgcccaa ggcctggagc actcgggcag ccaccaggaa gcggatgtcc tgtgccagcg
     1081 cgcacaggat ggagccgagc gtgaacagcg ctagccccgc cagcatcgcc ggccggcgcc
     1141 ccttgcggtc ggaaatcggg ccatagaaca attgcgccag cgcatagccg atcagaaagc
     1201 cggacagggt cgcctgcgtc tggctggtcg tggcatcgag agcagtgcgg atcaccggca
     1261 gcaccggcag atacatgtcg gtcgccatcg ggccgatggc agtcagcagg gccagcacgc
     1321 cggtcagcgt gagcgtatcg ggtttaaggc gagccacggc tgttccggta agggcgcgat
     1381 cagggcaggg gggcgaccgt cgcggcgtcg gacgcgccaa agcgatgtaa ccctgcgccg
     1441 tggcgagtca accagctttc ggaactctgc caatgcgggc acagcctcgc aacaaccgcc
     1501 cagtagcgcg gagaatggtt gagttcgacg cggtgggcca cttcatgggc ggccagatag
     1561 tcgagcactt caggcggagc caggatcagc cgccatgaaa acgacagcgc tcccgtggcc
     1621 gagcatgagc cccagcggct gcgcgtatcc ttgatggaga tccgggtgat gcgcacgttg
     1681 agctggcgcg cataggcggc tgaggcccgc gccagttcgg cccgggcttc ccgcttgaag
     1741 aaatctgtca gccgccttgc aagatggcgc ggatcgccgg caacgatcag acgtccgggg
     1801 tcgccatcca caccgggccg gcattcgacg ccgccgcgtg cgctgcccgc atgttcgatg
     1861 acatgcggca ggccgcggaa tggcacgatg catccgggtt caaacgcgac cgcttcgggc
     1921 agccttgcca tccgcgctgc caaccagccg ccgtgcctgt cgagaaaatc gcgcgcctgc
     1981 gacatgtttg tccgtcgcgg cagcgtcagc accgcctcgc cggtatcggc ccggaccctg
     2041 aggatcagcc gacgggccgt cgcattgcgc cggatggcaa caggatagct gcggccaagg
     2101 tgcagcactt ccagccgctc gatgcgtggc tttgccgggc gcttgcggaa aaaggacggg
     2161 ggccagtcga acatggcccc cgagcctaga agattcgcaa cactccgcct accggcgttg
     2221 ctcagccggt cttcttgccg ccattgatca gccgcggccg tccggggcgc tcgaagctgg
     2281 cctcggcctg ttccatgaag gcattcatcg ccggaacaat atggcgctgg aagcgcgagc
     2341 cgttgaacac gccgtaatgc cccacctttt cctgcagatg atagagctgg cggtgcttgg
     2401 ggatgttgac gcacagatcg tgggtggcct tggtctggcc gacgccggaa atgtcatcct
     2461 tctcgccttc gacggtcatc agcgcggtgc gccggatggc cgatgtgtcg accggcttgc
     2521 cgcgatgggt catcgtgcca ttcggcaatg catgctgcac gaacaccgta tcgatcgtct
     2581 gcaggtagaa ctcagccgtc aggtcgcaga cggcgagata ttcgtcgtag aaatcgcggt
     2641 gcttgtcagc gctgtcgcca tccccttcga ccagatggtt gaacatttcc gcatgggcct
     2701 cgacatggcg gtcgaaattc atcgccatga agcccgacag ttgcagaaat ccgggataga
     2761 cctcgcgcat gaagccaggg ttcgggaatg gaacgcgcat gatgcagttg cggcggaacc
     2821 agtcggtgcc gcgtttttcg gcgagcttgt tgacctccgt cgggctcttg cgcgtatcga
     2881 tcggcccacc catcagcgtt atcgagcagg gcgcgtgcgg atcgttctcc atttccatcc
     2941 gcgccaccgc cgccagcacc ggcacggacg gctgacacac ggcgatcaca tgcacgtccg
     3001 ggccgagatg gcgcaggata tcctgcacat agtcgatgta gtcatcgaga tcgaaccgcc
     3061 cctcggtgac cggcaccatc cgggcatctt cccagtccgt gatgtagacc tgatgggagg
     3121 gcaggaaggc ttcaaccgtg ccgcgcagca atgtggcgta gtgacccgac atcggcgcga
     3181 cgatcagaag cttggattgc ttgaggccgg ccgggtgggg accgcgcacg aagcggatca
     3241 gccggcagaa cggacgttcc cagacgatgc tctcggtcac cggcacctct tcgccctcga
     3301 tcaccgtatg gtggagaccg aaggccggtt tgccatagcg tcgcgtcagg cgctcgtaga
     3361 gttcgcatgc ggcggccagg ttacggccga aggacgtctg cgaaagcggg ttgagcggat
     3421 tgcggtagaa catcccgacc gtatccacaa aggcccgcga cggggagatg atcgaatgcg
     3481 ccgcctcgta gaggtagtac atgtggtggt tcatcaagac gcctttccaa tgctactttg
     3541 cccggtgttc cgggcgcctg ccgatgcccg ctcctgcaag ccgcgtgatt ttgacgcgca
     3601 accatgcgat tgaacactac agatcgaaat aaatccatta aacgattgct gcattgcgac
     3661 ataaatgcaa cgaggggcgg agttatcccc ggaagtgtgg catgggagct gagacaaaga
     3721 ctgctgcgcc ggtcgtagcg gacaccgcgc cggaccagat cctgcggcag aacacgctgg
     3781 tgcgcttgcg ctggctggct gtggctgggc aacttgtttc agttctggcc gttcatttcg
     3841 gcttcggttt tgccttgccg ctcgctgcgg cgcttgcggt gatcgcggcc tcggtggcgc
     3901 tgaatgtcgg gctcatgctc ggattttccg tcaaccatcg cctgtccgcc ctggctacgg
     3961 cggcggtcct tgccttcgat attgttcagc ttgctgctct gctggcgctg accggcgggc
     4021 tctccaatcc tttttcaatg ctgtttctgg cgccggtcat ggtttcggcc acggtgctag
     4081 cgccgcgata tacgctgctg cttggtgttc tggctgctgg cctggcctcg ttgctggcaa
     4141 tctggcactg gccgctgccc tgggccagcg atcaaccgct gacgctcccc ttgctgtatc
     4201 gggcggggtt gtgggcggcg gtgctgatca gcctggcctt tatcgccacc tacacgttca
     4261 aggttgccga cgaggcgcgc aaactggcca cggcattggc cgcgaccgaa ctggtgttgg
     4321 aacgcgagca gcacctgtca accgttgctg gtctggcggc ggctgcggcc cacaaactcg
     4381 gcacaccgct cggcaccatc accctcgtgg ccaaggaact ggatctgcag gtgccggcgg
     4441 ggcatcaact gaaagatgat atcacgctga tccggcagga ggcggcccgc tgccgcgaga
     4501 ttctgaccaa catttccgca ttggggcgcg atgatggcgg cccgctcacc cgcatggcaa
     4561 taagccaact ggtcgaggaa ctggtggcgc cctaccgccc cagcgatgtg gtgattgatg
     4621 tcaggcttga tggtccgcaa cccgagccga cccttagacg caacccggag ctgttctatg
     4681 cgctgggtaa tctgattgag aacgcgctcg attttgccaa ggatcggatc atcgttcgcg
     4741 ccctgtggac gtccgaaagc atcagtctgt cgatttcaga cgatggcccc ggcttttccg
     4801 tcgatgtcat ccagaggctg ggcgaaccct atgtcacaac gcgccgccac cgtttgaccg
     4861 aggctggcct gcgccgtcag gttggtggag gccatggcct cggcctgttc atttcaaaga
     4921 ctttgcttga acgcggcggc gggcggattc gctggtccaa cctgccggcg cctggcggcg
     4981 cgcataccga ggtgtattgg caccggtcag attttggccg caaatttgat cagagggggt
     5041 gacaatgcgg accgagtggg ctttatcatc cttggaacgg ggtcttggag agatgcgctc
     5101 gatgggggat atgagccttc tgattgtgga cgatgacaag ccgttcctga caagattggc
     5161 cgcagccatg agcaagcgcg gctatcaggt cacgatggct tgcggagtct ctgaaggctt
     5221 gcgcgcgatc atgacggcgc ccccggcttt cgctgtgatc gacatgcggc tcggcgacgg
     5281 caatggcctc gatgtcattt ccgaactcaa gcggatgcgg ccggacgcgc gggcgatcat
     5341 cctgaccggc tatggcaaca tcgcaaccgc ggtgaccgcc gtgaaactcg gggcgtttga
     5401 ttacctcgcc aaaccggccg atgcagacga gatcgatgct gccctgaagg cattgcccta
     5461 cagccggcct gaactgcctg aacatccgat gtcggcggat cgcgtgcgct gggagcatat
     5521 ccagcgcgtt tacgaatcgt gcaatcgcaa tgtgtcggaa accgcgcgtc agctcagcat
     5581 gcaccggcgc acgctgcagc ggatcctggc caagcgcgct ccgcgttgac cgacgcgggg
     5641 cgaaacgccc cgcaccaggc cggtcagctt gcgtcaaagt ccagcttgac caccggcgtc
     5701 tgggggcggg actggcaggc aagcacgaag ccggcctcca tttcccatgg ctccagcgaa
     5761 taattggcat ccatcgtcac cttgccctcg gtgaccttgc agcgacaggt gcaacacatc
     5821 ccgcccttgc aggaataagg cagttccaca ccctgggcat gggcggagtc gacgatccgt
     5881 tcgtccgggc gcacctcgat gtgataggtg atcccgtcca gctggcaatc aaccgtcgaa
     5941 acaccacccg tggcatgtgc ggcgctcggc ttgggcgcga cgcgcggggc accatcggtt
     6001 gaaaacaact cggccttgat gcgcttggca tcgacaccgg aagctgtcag cgccgcacgg
     6061 ccttcctcga ccatcgaata cgggccgcag aggaaatagg catccacctc ggccggcgga
     6121 accatggtct tggtcagcag cgccactttc gcctgatcga tgcggccgtt gagaatgtcg
     6181 atatcctggc tctcgcgaga gagaatgtgg aacaccgaca gccggccgag atagcgatct
     6241 ttcagatctt ccagcgcttc gcggaacatg atcgaggcgg tggtgcggtt gccatagaac
     6301 aggctgatgc gcgtcctggt attgccggcc agcgccgaac ggatgatcga cagaaccggc
     6361 gtgatgccgg agccggcggc gaagaacacc agatgacggt cgcgcgcggg gtcggcgaca
     6421 aagcgcccct cgggcggcag cacatccagc accgcgccgg gcttgagctt ttcgttggcg
     6481 aaggtggaaa ataccccgcc tgccactttc ttgactgcga tccgcagttc accgtcctga
     6541 aggccagagc agatcgaata ggagcgacga cattccgttc cgccaagcat ggtgcgcagg
     6601 gtaaggtatt gtcccggcgt gaaggagaag gtctgcttca gcgcctccgg caggttgaac
     6661 accagcgata cgctgtcggc agtctcgcgg cggatgtcct tgacgctcag cgggtggaaa
     6721 cggctcatgg atcggtcctc atacgcattt gaaatgatcg aagggctcgc ggcagcggcg
     6781 gcagcgccac agcgccttgc aggcggttga gccgaattcg gacaggcgct cggtttcggc
     6841 agaaccgcag cgcgggcagg taacatcggc ggaggcttca aacagtgccc gcttgccgct
     6901 ggcccgctcc ggcggcgaaa ttccataggc gcgcagcttt tcccgtgccg catcgctcat
     6961 catcgcggtc gaccatgccg gcgtctgcga cagtctgacg gcgaccttgt cgaacccgtc
     7021 cctggcaagc tccgtctcga tgccgagggc aatgacgccc atggccggac agccggaata
     7081 ggttggcagg atggtgaccc gcacaacgcc cgcctcatcg atctccacgc cgtgcagaat
     7141 gccgagatcg gcaatggtca gcacgggcac ttccggatcg gtaacgcggg ctgccgccgc
     7201 tctcgcgcgg ctcgccgcat catccggagc gggagcaggg acggccatct caccacaccg
     7261 cgtcgggata ggcgcgctgg aggtgctgca tcgtcgccag catgtggcca aggtgctccg
     7321 aatgcacgcc ctttcgcccg ccgcgaatgc cgtgacgatc cgccgggcgc ttcagcgtgg
     7381 cgcgcttcaa cacctcgctg atcgtggcat cgaaagcccc gcgcaaaccg gccggatcga
     7441 caccgaagcc gctggcagca acagcacggt cgagatcatc cgtctcgaac aattccggca
     7501 cataggccca gaggtcatcg agtgcttcct gcgcccgggc gtggctttcc ccggttccgt
     7561 cacccagccg gatcatccat tcggcggaat ggcgcagatg atacgccatt tcgttggcgg
     7621 agcgggtggc gatgcccgcc acatccgcat cggacgagcc tttcagggcc tcgtagagcg
     7681 gcagcatata ggcggaaaag atcaggtgtc gcgccatcgt ctcggcgaaa ttgccgttcg
     7741 gctgctcgac cagcagcagg ttgcggaact cctcctccgg gcgacgatag gcgagcatgt
     7801 cctcggtgcg gcccgccgct tcgagttcgc aggcgcgggt atagagcgcc cgcgtctgtc
     7861 cgatcaggtc gagcccgagg ttggacaatg cgatatcttc ctcaagcgtc ggggcacagg
     7921 atacccattc gctcatccga tggccgagga tcaggctgtt gtcgccaagc cgcatcagcc
     7981 aggcggcggg cagattgggt gaggcatggg tcatgacggc atcctcacat atggccgact
     8041 tcttccggca cctcgtagaa ggtgggatgc cggtagatct tggaagccgt cggctcgaac
     8101 aggacatcct tggccgacgg gtccgaggcg acaatggcgt tggacggcac cacccagatc
     8161 gacagcgtct cgccgcgccg ggtgtagaca tcgcgggcat tttgcaacgc catctcggca
     8221 tcgatcgcat gcagcgatcc gacgtgccgg tgggcgagcc cgttgcgcgg gcggatgaac
     8281 acttcccaga gcggtgtggc tttctcggtc atggcaactc tctcgtcgaa tagggcagaa
     8341 aggtgaatgg cgcgccgccc ggaacgtcag gccgccttgc gggcggcacg cttggcggca
     8401 tgggcgaaca atgcttcacg cacccaggcg ccgtccgcct cgacctgacg gcgatgcttc
     8461 atgcgctggc ggttgcaagg cccatcgccg ttgatgaccc ggacaaattc gtcccagtcg
     8521 atgtcgctgt ggtcccaatg gccggtcgcc ttgttgaatt tcagcttcgg gtccgggatg
     8581 gtcaggccga gatactgggc ctgcggcaca gtggcatcga agaacttctg ccgcagtccg
     8641 tcattggaga agcgcttgat cttccacttc atcgacactt ccgaatgcgg actgtcggaa
     8701 tcatgcgggc cgaacatcat catcgccggc caccagaagc ggttgagcgc atcctgcgcc
     8761 atcgcgcgct gctccttgga gccacgacac atcgccacca tgatttcgta gccctgcctc
     8821 tggtggaacg attcttcctt gcagatcagg atcatggcgc gggcgtaagg gccatagctg
     8881 caccggcaca gggcgatctg gttcatgatc gccgcaccgt ccaccagcca gccgatggcc
     8941 cccatatccg cgtagttgag ggtcggataa ttgaagatcg acgaatattt ggcccggccg
     9001 ctgagcagct gctggtaaag ctcctcgcgc gagacaccca gggtttcagc cgcagcatag
     9061 agatagagcc cgtgcccgcc ctcatcctgg atcttggcca agagctgcgc cttgcgcttg
     9121 agtgtcggcg cgcgcgtcac ccagttgccc tcgggctgca tgccgatcac ttccgaatgg
     9181 gcatgctgtg agatctgccg gatcaatgtg cgccggtatt gctccggcat ccagtcgttc
     9241 ggctcgatct tctcttcggc atcaatgcgt gcctggaatg ccgccagctt ttccggatcc
     9301 tccggcttga ccggggcaag gtccttcaca ttgagggctt gagcatacat gcctttgtct
     9361 ctccctgttc ccgtgggctt tcgctgaccg agcggtcaat tattaaatgc caatatgcat
     9421 caattatcag ctgatgcaag caaattctgt aacatgtcgg agtggccgcc gactggctcg
     9481 atcagtcgct catcaagcca ggattcggca gcgggcgaca ggctggcccg gagctcatgt
     9541 gccagcttgc gcgcggcaaa tcccggccag tcggccggca gatagcaggc tggcaggcct
     9601 ggatcccgga gcacgatccg gcggaagcga tgaatcatca gcaccctgag ggcgaccgcc
     9661 tcgagaccgt cggccggcgt atggccggcg gccaaatgac tggccagatc agcatgattg
     9721 gcaagaaaat tgccgtagga gcgtgccagt gcggccagat cgaaggcatt ggccacgacg
     9781 agccgcgcct gatcgtccgc caaacggctg acgggcagca gcagagcgcc gggctcgctt
     9841 cgcagcggat gatgatcagg caggatgaag ctgtgcgtgt ccagcgtgcc ggcgccctgg
     9901 cgcagcagcg tctcacgcag gcgccgatgg ccgtcgggag gcagtccggc ggtgatcatc
     9961 acctgccagc cgcacggcgc ctggggcggg tctgccgcat agatgcggcg ggaggctgcc
    10021 tgcgattcgg ccagcgcggt gggcgtcagg gtatagtagc tgttgcggcc cgagcgcacg
    10081 cgcgccaccc atccgtcgga ggcaaggcgg gacagggcgg ttcgcaccgc cccggcgtcg
    10141 atgccgcaag cgccgagcac gtcgctgatc gtcgacaggc cgacaagccc gccgcggggc
    10201 acgatacagt ctccaaggaa gctgacggcc agtgaccata tccgcagcgt tccccgtggt
    10261 gcggaggtca ggcagcggct caacagggag tactgggaca ttgcctcggt cattgagacg
    10321 gtcatcttcg cccgcccagc aggcgtaggc aagcgctctc gatcacgccg cattttggtg
    10381 aaatcatcaa aatgcggata acgtgatcgg tatcaataag ttagagcatg tcttctgcga
    10441 ctaaccggca tccacttaat cgcgacatgc tctagtcccc cggcggcgat atgcggcggc
    10501 gggccacccc atgggtcgtc ttgttccagc gatgcggctg gtgcagcagt tcccaaagtg
    10561 ccttgtaggc cgcaagcgag atcaacatta gatagagcgg cagcagcatc agccatgggg
    10621 ccagccgcca gaggtcccgc cgcgccatgc cgatcatgag cggcaacaca agggcaatga
    10681 ggccggccac aacaagcccc agcatcacgc catcggccag cacatgcacc caatttccgc
    10741 tgaagaatgg cgcgggggta gcaaaacggc gggcaagaag cgccatgaaa accggcgcgc
    10801 cgagcgcccc caggatgatc ccggccgatt gcgccacggc ggccatgaac gacagcggtc
    10861 caagtgcttt gaaggctgtg cagggggccc ggctgtgggt aatcgcggtc tgcatccatc
    10921 ccttcagcca gcggctgcgt tgatgcagcc atgccccaag ccgcaccggt gcttcctcga
    10981 atgtcgtcgc gttgagatcg gccacatgat agccatgcat ggcgaggcgt atgccgaggt
    11041 cggcatcctc ggtcacattc caggcatccc agccgcccaa ctggcgcagc acgtcggtgc
    11101 ggaagtggtt cgacgttccg cccagcggca ccggcagtcc aagccgcgcg gttccggcct
    11161 tggtgacgtc gaacaaggcc gcatactcga acgcgaacag ccgtgtcagc atgctgtcgt
    11221 cggcattgtc gatcgccagt cgcgcctgca gacaggctgt actctggggc agggcggcaa
    11281 acatcgccgc ggccttgcgc agttgctggg gttcaggtcg atcctccgcg tcgaagatca
    11341 ccacccgttc accggtggcg aagggcaggg cggcattcaa cgcgcggggc ttggtatgtg
    11401 gcgcgcccgc cggcgccacg aacacctgca tatgggacgg cagtgacagg ccggccagtg
    11461 cagcctgggt ctgcgcatca tgctcctcga caatcagcag cacctggagc ttttcggccg
    11521 gatagtcgag ccgggccagc gcggcgacga gatcgccgat gatttcggcc tcgcgataga
    11581 gcggcaccag caccgaataa agcggcaggt tctcatcgtt ctgttccggc cggatatcca
    11641 cgatgggcag cccggccccg accgccgctg cctgcagccc agccatgacc aggaaaacca
    11701 cgcccagcag catcggcgcc agcagaaggg ttgcttcgaa tgcctgcatc gctacggctg
    11761 ccaaggcgct gacggcgatc cccagcactg ccatctgcct gttggtcaag ccgccgcgcg
    11821 ccgattcgtc cgccggcacc gaatgggccg ccgcataggc gatggcctcg ccgaaatggc
    11881 gcttgaggac atcgtggtag ctggcccgct ccagaacgac cacatggcgg gcatgatggg
    11941 ccagcgcgcc ctggccaagc agacgcgcca ccgggccgat cgcggcgatg gccagcacgg
    12001 gccgcccgtc ggcggttgtg gcacggaacc agccctgacg cagcgcgtcc tgatgccgga
    12061 cgcgtgggtg aggcacaaga ttggttggct cgttgcactg gagatgccgt gccagcagac
    12121 gggcatagac cgtctccgtc gtcaacccgg aagcgaccag cacctccgca gcagcagcgc
    12181 caatacgccg ggcacgcgct gcaatcggct tcagcgaggc ggccgaaaaa ccaaagcgga
    12241 caagaaaggc gatgtcagcc ggcagcggca gggtcggcaa cggcagttga acgggtagtc
    12301 gaacacccac gaccagagcg tctgcattcc gctcttgcct ctgcgtcgca gtcacgccaa
    12361 ggtccaacgc catggctgcc accatttacc cgaccgaccg cagggacacc ctgcccctcg
    12421 tgcatggatg caagactatt gtttgcaatt tggccttgtc cagcatcctc ctggccctgg
    12481 tcgtgaatgt cgcagggaca acgcccgtta cggctgatga tacagtggcg attccaaacc
    12541 tatgggattc tcggcgcaaa tttgacccac ccaatctcgg gagccagcgg cagatccgct
    12601 ttatcaccga gagcgattac ccgcccttcc actttgtcac gccgagcggc cagctgtccg
    12661 gcttcgaggt cgaccttgct cgcgggttgt gtgacctgct caaggtgagc tgctcgatcc
    12721 aggcggtgcg ttgggatggg ctcaaggacg cgcttatgac cgggcagggc gatgcaatca
    12781 tcgccgcgat gcgcattacg cctgaggcgc gcaagacgtt tggtttcaca accgcctatt
    12841 tccgcagccc ggcccggttc gtggttcagc gcgccgatcc tctggttgag ccgacgctgg
    12901 agcagctgtc cggcaagcgc gtggcggtta tgggccagtc ggcgcatgaa gcctatatga
    12961 ggacgttttt tcccgacgtc gggcgcatgg tgacgcagaa tgccagcgag gccctggcgg
    13021 cggtcagagc cggtgaggtg gcggctgcct ttgtcgacgg cacggttgcg gcgttctggc
    13081 tgaatggcga acaatcgggc aattgctgcc gttttcttgg cggggggttc actgaggcgc
    13141 ggtttttcgg cgagggggcc ggcatagccg tccggccgga tgcgcaggct ttgcgccagg
    13201 cgcttgactg ggcgctgcac cgctttgcag ccgaaggagg gtatgcccgg ctctatctca
    13261 agtatttccc ggtcagcatc tactgacagg gcttacggcc cggccacgat gttgcatcgc
    13321 accaaaggga attgacgcct gagccgctcc tgacctacgg tcgcgcgccc gatgcatgga
    13381 gaacaaagca ccgatgtccg cccccgaatt gcgtgaagct gccctgaccg ctgctgcctg
    13441 gccgttcgag gaagcgcgca aactggtggc gcggatcgag cgtacaggtc aggattcggt
    13501 gatcttcgag accggttacg gtccgtcggg tctgccgcat atcggcactt ttggggaggt
    13561 tgcccgcacg acgatggtgc gtcgcgcctt cgagcttctg accgacggca gggtcaagac
    13621 ccggctcatc gccttttccg acgatatgga cggattgcgc aaggttcccg acaacatccc
    13681 caacagggag atggtggcag ggttcctcgg ccgaccgctg accgaggtgc cggatccatt
    13741 cggcacgcat ccgagcttcg gcgcccacaa caatgcccgc ttgcgcgcct ttcttgacac
    13801 cttcggcttt gactacgagt tcaagtcctc gaccgaatgc taccggtccg ggctgttcga
    13861 cgcgacattg ctgctgtttc tgcgccgcct cgacgatgtg cagaagatca tgctgccctc
    13921 gctgcgcgag gaacgcgcgg cgacctattc gccgttcctg ccggttcacc cggaaacgcg
    13981 ggtggtgatg caggtcccca ccgaggcgcg cgccgaagct ggaacgattg tctggaccga
    14041 tccggatagc ggcaagactt atgagacgcc ggtgaccggc gggcattgca agctgcaatg
    14101 gaagcccgat tgggccatgc gctggatggc gctcggggtc gactatgaga tggccggcaa
    14161 ggatctgatc gattcggtca agctctcctc ggccatcgtg cggcgcctgg ggggctctcc
    14221 gcccgagggg ttcaactacg aactgtttct cgacgagggc ggccagaaga tttccaagtc
    14281 gaagggcaat ggtctgacca ttgacgaatg gctgacctat gccagccctg aaagccttgc
    14341 gctgttcatg tatcagaagc cgcgcgaagc caagcgcctg catttcgatg tgattccgcg
    14401 ctgcgttgac gaatacctgt cgtttcttga cgcttatccg cgtcaggacg tccgcaaccg
    14461 gctgggcaac ccggtctggc atatccacaa cggtgcgccg cctgccgctg agacgcttgg
    14521 acaggatccc tcggccaatg caccgcgcac cgccggtctg tcgtttgcat tgctgctcaa
    14581 tctggtggcg gtcgccaatg ctgaaaccaa ggatgtgttg tggggcttcc tgcagcgcta
    14641 tgcgccaggg gtctcggccg cgacgcatcc caggatcgat gcgctggtcg gctatgcgat
    14701 ccgctattac gccgatttcg tcaaacccgg gaaggtctat cgcgaggccg acgaggttga
    14761 aaccgcagcg ctttcggctc tggcccgagc gctggctgcg ctgccccccg attcgaccgc
    14821 cgagcgtgtg caggaggtgg tctacgatgt agcccgtccg attccccgct ttcaggacat
    14881 gaaggccaag ggcgccaccc cggagcggcc cggcgtctcc aacctgtggt tcaacacgct
    14941 ctaccagatc ctcctgggag aggagcgggg gccgcggttt ggatcgtttg ccgcgatcta
    15001 cggccttggc aacacagtgc ggctgatcga gcgggccctt tcgggtgcgt tgcgcaagga
    15061 ccatgcggcg tttttggaga gccgcgccgg atagttcggc tgcgccggtg cgcagccgtc
    15121 agcacctact ggctcgccca gcggatccgg ccgaccagtt ccgcctcgct ttttgtgacc
    15181 gtaaccatgc cggcgtcacc gccgaacgga atgatgtcga tgccctgcgc ggtttcccgc
    15241 cgcatctcgc caaggatcac ccgcccggca gccgtgcgga tgaaaacgcg gtcgccgcgc
    15301 cgtagcgcat cccgtgggac gatcaccaga acatcaccct cgcgataggg ggcagctccg
    15361 ctggcaagtt cgagcgcgaa tgcctgcgta tctgctgctg ctggcaggga tatatcactc
    15421 cagtccggac cgctgggcct gcctgtgtcg tcgaaactgc ccggcagcgc tgccttttcg
    15481 ctgtcgatca ggggcaggcg catgccggcg gcttgctgac cctgcggacg gatcaggctg
    15541 acaaaatcat cgataccggc tcctgtcgcc ttcaggatct tggcgatcga ttcggttgat
    15601 ggccagcgct cgcgtccatc aggagtcagc cgtttcgact tgttgaacgt ggtcggatca
    15661 agaccggccc ggcgcgccag ccccgaggcg gagaagccaa acttttccgc cagagcgtca
    15721 accgcagtcc agatttgttg atgggtcagc atggtacctc caaggaacct ttccctgaca
    15781 gaggaatttt tgcctgtaaa gggtcgtttt ttagtttatt cgtctcggat gggcgcggac
    15841 agtccttgcc ttgtgtgtcc agcgaatgca cagacacgct ggaacagggg ccagcgatgc
    15901 cactcattta caagatcatg cctgccacga tgtggcgcga cgcggtcagc gctggtgcct
    15961 tcaccggcgc accggtcgac cacgcggacg ggttcatcca tttctctgcg gctgatcagg
    16021 tgcgggcgac ggccgcaaag cactttgctg ggcatcggga tcttgtgctg gtcgctgtga
    16081 cggccgaagc gcttggatcg gcgttgaagt gggaggtctc gcgcggggga gccttgtttc
    16141 cacatcttta tgccccgctc gtgataacgc aggatgtgct cgtcgagccg cttcccctgg
    16201 gagccgacgg gctgcatgtt tttccggagg caattccgtg aaggattttc tgtttggcct
    16261 ggcgcgtcct gtcctgatgg gacttgatgc cgaagccgcc catcgggcaa cgattgcggc
    16321 cctcaaggtc gcaccggaat tgccgctgcc gatcgatccg gatgtcctgc atgtttcggc
    16381 cttcggcctc gactttccca atccggtagg gctggcggcc gggttcgaca agcatgccga
    16441 ggttgccaat gcgctgtttg gcctcggctt cggctttgtc gaggtcggtg cggtgacacc
    16501 gcgtccccag ccgggcaatc cgcgcccgcg agccttccgt ctgatgtcgg atgaggcggt
    16561 gatcaatcgc tacggcttca acagcgacgg gctggatgtg gtggccggtc ggcttcaacg
    16621 attgcggccg cgcggtatcg tcggcgtcaa tcttggtgcc aacaaggaca gcgcagaccg
    16681 cgctgccgat tatgaggtgc tggtgcggcg gttggccggg cattgcgcgt tcctgaccgc
    16741 caatgtctcg tcaccgaaca cgcctggctt acgctcgatg cagtcccgag cgccgcttga
    16801 cgacctgctc cgccgagtga tcgatgcccg cgatgcggcc tcagaggggc ggagccgcac
    16861 gccggtcctg gtcaagatcg cgccggatgt gaccgatgag gatctcgacg acatcgtcgc
    16921 cgttgcccgg gaacggcagg tcgatggcat ggtgatctcc aacaccacga tcgcccgccc
    16981 cgccagcctg cgcgatcagg cgctggccgg cgaggccggc ggcttgtccg gccggccg
//
LOCUS       DAOMXG010000035        16473 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_1987_length_16473_cov_9.076136, whole genome shotgun sequence.
ACCESSION   DAOMXG010000035 DAOMXG010000000
VERSION     DAOMXG010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 16473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16473
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1987_length_16473_cov_9.076136"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="PLQ11_04000"
     CDS             <1..770
                     /locus_tag="PLQ11_04000"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRK24098.1"
                     /translation="ALRGAIAAVAVGGFLLALRQVPLPRGDELLPWSVIGLFNGAIPN
                     VLVAFAMQRMDSGPAVLIQAIGPLLTAVAGHLLFAEERLNARSLAGVAVGFCGVGLLI
                     GPAAFEGKAPLPAMLAMLGVALCYTIGNIYTRNLSHHDPMRLALGQQVASAVFAGLAA
                     LTTGGIAAFAGADRHMPALLALGIFCTAVPISVFYRLIVRAGPTRASLAGYTVPAVAV
                     AIGALVLGERLTLWQAAGGLTVLAGVFLVATSKRKAP"
     gene            767..1165
                     /locus_tag="PLQ11_04005"
     CDS             767..1165
                     /locus_tag="PLQ11_04005"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HRK24099.1"
                     /translation="MKLVLVVAVALVDADKRVLLARRPEGKQLAGLWEFPGGKLNDGE
                     RPEQALIRELKEELGIDVKEPCLAPLTFASHAYETFHLLMPLYVCRRWEGLPRALEGQ
                     TLAWVRLNDLRSYPMPPADEPLIPILRDLL"
     gene            complement(1172..1624)
                     /locus_tag="PLQ11_04010"
     CDS             complement(1172..1624)
                     /locus_tag="PLQ11_04010"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017486592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRK24100.1"
                     /translation="MTGQTKPTRKPRWQRSQFRRFVPMPTRWDDNDAFGHVNNVKYYA
                     FFDTAITFVEIEAGIFNSGSSPLVPYVVDSSCTYFEGVAFPDVLEVGVAIERIGRTSL
                     TYRLGVFRPGADLPVAQGLFTHVYVDRATERPAPMPDAVLKLAESLLS"
     gene            complement(1621..2517)
                     /locus_tag="PLQ11_04015"
     CDS             complement(1621..2517)
                     /locus_tag="PLQ11_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRK24101.1"
                     /translation="MTDVPRLFDRALVRRHQGRALAALPATFLLDRAAEDLGDRLQTV
                     TRGFSRIVDLGTPGAGAADVLAARFAGATVTRIGDVVALRPDRLVEDLEAPALGQECA
                     DCVVSLLALQTVNDLPGLLMQIRRALVADGLFIAALAGGDTLKELRACLLEAEVEETG
                     GAAPRIFPFADVRTLGQLLQRAGFALPVVDSETLVVRYGDPLRLLADLRAMGAANALA
                     DRSRRPLRRAVLMRALELYRARFADADGRVRATFEIVWLSGWAPHESQQKPLKPGSAR
                     VRLADALRQPDYAKPATKSEAP"
     gene            2593..3396
                     /locus_tag="PLQ11_04020"
     CDS             2593..3396
                     /locus_tag="PLQ11_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HRK24102.1"
                     /translation="MTSQTLPPPSAGVPAGPAAPAIAGRWLARFGRRTLDVVYPAHCP
                     GCGSAVADAGALCGPCWQTMPFITRPFCERLGTPFAYDQGGSLLSPAAIADPPVFDRA
                     RAVARHDGLARSMVHRLKFSDRLDLARPMGGLMAVAGKDVLAGADALVPVPLHWTRLL
                     WRRFNQAEVLADWVGRESGLAVLARTLRRRKHTPQQVGLSRPQRIANLQGAFVVRPDQ
                     AAGIEGKHLVLIDDVHTTGATLNACARVLRRAGAARIDVLTFTKVADEA"
     gene            3452..3715
                     /gene="grxC"
                     /locus_tag="PLQ11_04025"
     CDS             3452..3715
                     /gene="grxC"
                     /locus_tag="PLQ11_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HRK24103.1"
                     /translation="MVTPEITIYTKGYCPFCQDAKALLQRKGAAFTEIDVTADRDRHA
                     EMVQRAGGRRTVPQIFIGTIHVGGCDDIYALDAKGGLDSLLKG"
     gene            3719..4156
                     /locus_tag="PLQ11_04030"
     CDS             3719..4156
                     /locus_tag="PLQ11_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012317073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HRK24104.1"
                     /translation="MIRYALACEAGHGFDSWFRSSEDFDAQCARGLVACPYCHSTTIA
                     KQVMAPIVARSDRGEPAAPAPLALIDDKAAELRRRIAELRTYVAENCENVGPRFAEEA
                     RRIHYGESETRSICGEASREDAAALIDEGVPILPVPVLPDDRN"
     gene            complement(4163..4909)
                     /gene="ubiG"
                     /locus_tag="PLQ11_04035"
     CDS             complement(4163..4909)
                     /gene="ubiG"
                     /locus_tag="PLQ11_04035"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019906892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HRK24105.1"
                     /translation="MNARASNSVDEREVSQFDRIGADWWDENGPMKPLHRINPVRLSY
                     IRGAIAARFGRNAQALDALRGLSILDIGCGGGLLCEPLARMGAAVTGIDPAPNNIAVA
                     RAHAEGEGLAVSYVEGLAEDLVAGGRTFDVVLAMEVVEHVPDVAGFVATAAALVRPGG
                     LFLGSTLNRTKRAYALAILGAEYVLRWLPVGTHRWEQFVTPEEFAEALTVTGLGEVET
                     RGMVYNPLADRWHLSSDTAVNYFIAATRPA"
     gene            5062..6297
                     /locus_tag="PLQ11_04040"
     CDS             5062..6297
                     /locus_tag="PLQ11_04040"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRK24106.1"
                     /translation="MPRLVMKFGGTSVANVERIKNVARHVKREVDAGNDVAVVVSAMS
                     GKTNELVAWCKETDPLHDQREYDTVVASGEQVTSGLLAIALQGLGLKARSWQGWQIPL
                     YTDAAHGAARIAGIDGSGILAGFAKGEVAVISGFQGIHKETGRIATLGRGGSDTSAVA
                     VAAGLKADRCDIYTDVDGVYTTDPRIVKAAHRLDKVSFEEMLEMASLGAKVLQVRSVE
                     LAMVHNVRTYVRSSFDDPANPHPGTLICSEEEILEQQIVTGIAYSKDEAQISVRGVAD
                     RPGIAAAVFEPLAAGHIVVDMIVQNVSEDGKSTDITFTVPSSDYDRALAVLGQLKGEI
                     GYRALQGAKDVAKVSAIGIGMRSHSGVAAKAFKALADRGINLRAITTSEIKFSVLIDE
                     EFTELAVRTLHHLYGLDAA"
     gene            6310..7299
                     /locus_tag="PLQ11_04045"
     CDS             6310..7299
                     /locus_tag="PLQ11_04045"
                     /inference="COORDINATES: protein motif:HMM:NF020804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit Rsm22 family protein"
                     /protein_id="HRK24107.1"
                     /translation="MAHDLPNALRQAIAEHTGSLANGLAEARSELTAGYRSGAASRIA
                     MTSQRHIAAYLIARLPATYAAVAAALERLAGQWSGFAPKSLFDAGCGPGTGLFAALVA
                     FPSLDQLAGLDRDAALIEVARDLVTRSGLAGDRPVTFTTDDLARPGLALAPADLINCT
                     YALVECDEDDIGRIAATLFAAARQALVLVEPGTPAGFERLRAARTALIGQGAQILAPC
                     PHQSACPITAPDWCHFSVRLARSRDHRRLKDADAPFEDEKFSYLIAARAPGANQTGGR
                     ILRPPRHGKAGSRFDLCTDAGNIQSRTVPARDRTLHRSVRDLVWGDFLPEVPD"
     gene            7440..9707
                     /gene="ptsP"
                     /locus_tag="PLQ11_04050"
     CDS             7440..9707
                     /gene="ptsP"
                     /locus_tag="PLQ11_04050"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HRK24108.1"
                     /translation="MRGGVGPRVLLRRLREVMAEPVSPQERLDRIVVLIAANMVAEVC
                     SVYVLRADGALELYATEGLKREAVHQTTMRAGEGLVGLIAREARALSLSDAQSHASFS
                     YRPETGEEIYASFLGVPILRAGDTLGVLVVQNRASRSYSEEEVEALETAAMVMAEMLA
                     TGELEALARPGSGGIALRRTLALQGRPINDGIGLGHAVLHEPRAVVTNIVADDVEAEA
                     RRLDEAIETMRASIDDLIDQGNVSHAGEHRDVLETYRMFAYDRGWLRRLREAVMSGLT
                     AEAAVERVQSDARARMLRQTDPYLRERLHDLDDLANRLLHQLTGSAFTLHASELPENA
                     ILVARSMGPAALLEYQRSKLRGLVLEEGGATSHVAIVARALGVPCLGEVEGLTSLIQP
                     GDAIIIDGTSGDVHVRPPPDLVAAYAERARLRAGRMELYRKEKTLPSVSKDGIKVDLL
                     MNAGLLVDLPNLADTNAAGIGLFRTEILFMVAPTLPRASTQEGLYRSILTIAGSRPVT
                     FRTLDIGGDKVLPYLRKVEEENPALGWRAIRIGLDRPALLRTQMRALIAAAADRDLRI
                     MLPMVATPAEFDAARTLLDRERERTRRLGRPQPRSIKLGAMLEVPSLLFDLDALLRRV
                     DFISIGSNDLMQFLFAVDRDNARVATRFDDLSPAFLRALRLIADKAVAANVPVTLCGE
                     MGGRPLGALALFALGFRSLSMAPARIGPVKSMLRALNIGETSRWLAEILDSDISGEAL
                     KQRLTAYAEANGIPL"
     gene            complement(9862..10389)
                     /gene="hpt"
                     /locus_tag="PLQ11_04055"
     CDS             complement(9862..10389)
                     /gene="hpt"
                     /locus_tag="PLQ11_04055"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HRK24109.1"
                     /translation="MIERPIRVLYDETEIARRVRELAHAIAAARPERLLVISVLRGSF
                     IFAADLLRAMHREGMSPQVEFMQLSSYREGTVSSGQVTITKDIEGDVRGSDVLIIDDI
                     LESGRTLAFAKDLLMARGARKVMVAVLVEKPGHRAVQIDADFVGFVSPDLFLVGYGMD
                     VAHSYRELPFVGVVD"
     gene            complement(10503..11204)
                     /locus_tag="PLQ11_04060"
     CDS             complement(10503..11204)
                     /locus_tag="PLQ11_04060"
                     /inference="COORDINATES: protein motif:HMM:NF023334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HRK24110.1"
                     /translation="MLLIKCPACAASYELPESSFGPEGRKVRCTACRTLWLARPAGSE
                     EPAEPAAEAIEETEGATPVPQMMPVERPRPWHQRIRPAGQTSSFRRPLVIVAALAACL
                     VVAVGLRKSIVERLPDLGRVYAAFGLPVNLSGLALRQIKGGLVQEGNVELLVVQGEIV
                     NVSPLPRPVPRLKFALMDAKGHEIYSWTAQPDTKDLQPGTMQSFRRRLASPPPEAVEV
                     LVRFASKDDLLALAN"
     gene            11345..12004
                     /gene="ftsE"
                     /locus_tag="PLQ11_04065"
     CDS             11345..12004
                     /gene="ftsE"
                     /locus_tag="PLQ11_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HRK24111.1"
                     /translation="MVRFENVGLRYGMGPEILKDLSFDVPPHTLQFLTGPSGAGKTTL
                     LRLILQTLRPTRGLISLFGHDTSSLDKDAITRLRRRIGVVFQDFRLLDHLTTYENVAL
                     PLRVQGRDERIYRPEVIELLKWVGLGDRMHVSPAILSGGEKQRAAIARATISRPELLL
                     ADEPTGNVDPSLARRLLRLFIEMNRSGTSVVLATHDLALMDQVDARRMVLAEGRLHVY
                     D"
     gene            11991..12956
                     /locus_tag="PLQ11_04070"
     CDS             11991..12956
                     /locus_tag="PLQ11_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRK24112.1"
                     /translation="MSMTEAPEVVTRPAGLAERLGLGGRRGTPLLPDSSLAGRALIIV
                     IIIMAFLASITAGTVQLIAQASSDWTGTVAREMTVQVRPRSGRDLEADVRRAADIAGR
                     VPGITEVRIFTRAEAERLLEPWLGAGLALGELPVPRLIVLRLDPAGQADVARLKGDLG
                     AVPTASLDDHRLWLKRLATMARTLVVIGLVVMALVLVAMALAVAFATRGAMAGNREII
                     DVLHLVGAEDRFIAREFQRHFLKLGLRGGAIGGGLAVLAFLLASSLSQRWIATPGGEQ
                     VEALFGSFALGFGGYAAVAVIAGLVAAITAVVSRTTVFRNLRGLD"
     gene            13267..13959
                     /locus_tag="PLQ11_04075"
     CDS             13267..13959
                     /locus_tag="PLQ11_04075"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HRK24113.1"
                     /translation="MASAPLPPSDLPPGLAPDAPVEPVGWSRRRRLAAALLALTGLAS
                     VAVYGGFLVFVSSLSRQETEFGRTVDGISALTGGAERIVDALAVLSEGRAKRLLISGV
                     NLQTSRASLIRQSGHAELFECCVDIGRAALNTVGNAIETAEWARRNKYASVMLVTSNY
                     HMPRALVELRRNLKNVEIVAHPVITEQVRVETWWHDPALFRLLFHEYLKYLFSEMRSR
                     LVPDNPPAGRGA"
     gene            14019..14780
                     /locus_tag="PLQ11_04080"
     CDS             14019..14780
                     /locus_tag="PLQ11_04080"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRK24114.1"
                     /translation="MLLLRNSLFAVAFYANCAFWFLFACFGYVIPARHFGFFCRGWAT
                     TSIWLFRTIIGAEIEVRGQHNIPHEAALVAAKHQSSFETIFLLIVLDRPSYIYKRELG
                     YVPLFGWHLLRAGQIPVDRDGGAEAMASLVARSRRALGEGRQIIIFPEGTRRAVDAPP
                     DYKLGVAQLYRTLGVPCVPVALNSGLAWPRRAFMKEPYRVVVEFLEPIPAGVPAKTAF
                     RRLQEDIETASNRLVDEALAAAGQTRAEIQSKSES"
     gene            complement(14741..15265)
                     /locus_tag="PLQ11_04085"
     CDS             complement(14741..15265)
                     /locus_tag="PLQ11_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003839 - gamma-glutamylcyclotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HRK24115.1"
                     /translation="MLHQHGDLWVFGYGSLIWRPGFDYVERHRALLKGAHRSLCVYSH
                     VHRGTRDQPGLVLGLDHGGACRGVVFRVDAHKKAETIAYLRAREQVNMVYKECVRPVA
                     LDDGRMVLALCYVVDRGHHQYAARLSREDVLRLVRQGVGQSGPNPEYVRNTALALKDL
                     GIHDSLLDWISARV"
     gene            15335..16369
                     /locus_tag="PLQ11_04090"
     CDS             15335..16369
                     /locus_tag="PLQ11_04090"
                     /inference="COORDINATES: protein motif:HMM:NF021423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2125 domain-containing protein"
                     /protein_id="HRK24116.1"
                     /translation="MDTAAPAPQRSRKGLYIPLILFALACLGWAGFWFFAKSRALDVM
                     DAWLAREARLGREWTCPQRTAGGFPFRFEVSCTGPTFASAEPGRAGKGSLGALTVTAR
                     VVDPRQVIAAATGPLKWTSEVGDSVEITFDGARASYRGSGSALEELSIDLANPLFTYT
                     APGLFTEKAAAGRAEFHMRRAPGEEAATDLVLIASAIKSALADQLTGETANGRIDLRT
                     RLTKLLPSPPRNWRETLELWRVAGGEARVEKLALAKGPIVIEATGALRLDETRRLDGE
                     ITGSAQGLPQILQAFGLGGNGGGLLGSLLSGGRPQQAQPAKALPFALRFAEGRLFIGP
                     IPGPRLRPLF"
BASE COUNT         2749 a         5508 c         5239 g         2977 t
ORIGIN      
        1 ccgcgctgcg cggcgcgatt gcggcggtgg ctgtcggcgg gttcctgctg gcgctgcggc
       61 aggttcccct gccacgcggc gatgaattgc tgccgtggtc tgtcatcggc ctgttcaacg
      121 gcgccatacc caatgttctc gttgccttcg ccatgcagcg catggattca ggcccggcgg
      181 tgctgatcca ggcgatcggg ccgcttctga cggcggttgc cggccatctg ctgtttgccg
      241 aggaacggct gaatgccaga agtctggctg gcgtcgctgt cggcttctgc ggcgtcggcc
      301 tgctgatcgg ccccgccgcc ttcgagggca aggcgccgct gccggccatg ctggcgatgc
      361 tcggcgttgc gctgtgctac acgatcggca acatctatac gcgcaatctc agccaccatg
      421 atcccatgcg gctggcgctt ggccagcagg tcgcctctgc ggttttcgcc gggcttgcgg
      481 cgctgaccac cggtggcatc gccgcctttg caggcgcgga ccggcacatg ccggccctgc
      541 tcgcgctcgg catattctgc acggctgtgc cgatttcggt gttctaccgg ctgattgtcc
      601 gggccggccc cacccgcgca tcgctggccg gctacaccgt tccggccgtc gcggtcgcga
      661 tcggcgcgct ggtgctcggc gaacggctga cgctgtggca ggcggccggt ggcctcaccg
      721 tgctggccgg ggtattcctg gtggcaacat cgaagcggaa ggcgccatga aactcgttct
      781 cgtcgttgcc gtggcgctgg tcgatgccga caagcgggtg ctgctggccc gcaggccgga
      841 aggtaagcag ctggccggat tgtgggagtt tcccggcggc aagctcaatg atggcgagcg
      901 gcctgaacag gcgctgatcc gcgaactgaa ggaggaactc ggcattgacg tgaaggagcc
      961 gtgcctcgcc ccgctcacct tcgccagcca cgcctacgag accttccacc tgttgatgcc
     1021 gctttatgtc tgccgccgct gggaagggct gccgcgtgcc ctggaagggc agacgcttgc
     1081 ctgggtgcgg ctcaatgatc tgcgctccta tcccatgccg ccggcagacg agccgctgat
     1141 cccgatcctg cgcgatctgt tgtagcagcc gctacgaaag caggctttcg gccagtttca
     1201 acaccgcatc gggcatcggc gccgggcgtt ccgtcgcccg gtcgacatag acatgcgtga
     1261 acagcccctg tgccaccggc agatcagccc cggggcggaa cacccccagc cgataggtca
     1321 gcgacgtgcg gccgatccgc tcgatggcga cgccgacctc aagcacatca ggaaaggcca
     1381 cgccctcgaa ataggtgcag cttgaatcca cgacataggg caccagcggg ctgctgccgg
     1441 agttgaaaat gccggcctcg atctcgacga aggtgatcgc ggtatcgaag aacgcgtagt
     1501 atttgacatt gttgacatgg ccgaacgcgt cattgtcgtc ccagcgggtc ggcatcggaa
     1561 cgaaccggcg gaactggctg cgctgccagc gcggcttgcg tgtcggtttg gtttgtccgg
     1621 tcatggtgcc tcggattttg ttgccggttt agcatagtcg ggctggcgca gggcatcggc
     1681 cagccgcacc cgcgccgagc cgggcttgag cggtttctgc tggctttcat gcggcgccca
     1741 gccggacagc cagacgatct cgaaggttgc ccgcacccgc ccatcggcat cggcgaagcg
     1801 ggcccggtaa agctcaagcg cccgcatcag cacggcccgg cgcaacggcc gccgcgatcg
     1861 gtcggccaac gcattggcgg cgcccatcgc cctgaggtcg gccagcagcc gcaagggatc
     1921 gccatagcgc acgacaagcg tttcgctgtc gacaaccggc agggcaaacc ccgcccgttg
     1981 cagcaactgg ccaagcgtgc gcacatctgc aaacgggaag atgcgcggcg cagctccgcc
     2041 ggtttcctcc acctcggctt cgagcaggca ggcgcgcagc tccttcagcg tatcgccccc
     2101 ggcaagcgcc gcgatgaaca acccgtccgc caccagcgca cggcggatct gcatcagcag
     2161 gcccggcagg tcattgaccg tctgcaaggc cagcagcgac acaacacaat cggcgcattc
     2221 ctgccccagc gctggcgctt cgagatcctc aaccagccgg tcgggcctga gcgccacaac
     2281 gtcgccgatg cgggtcaccg tcgccccggc aaaccgcgcg gccagcacat cggccgcccc
     2341 cgcccccggc gtgcccagat cgacgatgcg ggaaaagccg cgggttactg tctgcaacct
     2401 gtcacccaga tcctcggcgg cccggtcgag caggaatgtc gcaggaagcg cggccagcgc
     2461 ccgcccctga tggcgacgga caagcgcacg gtcaaacagg cggggcacgt cggtcatgca
     2521 ttctggataa gcgcggcgcc gggttgaggc aacctctgcc ggccgaggcc acttcgtgct
     2581 agagcatttt tcatgacgag tcagacgctg ccgcccccct ccgccggcgt tcccgccgga
     2641 cctgccgcgc cggcaattgc cggacgctgg ctggcacggt tcggccggcg cacgcttgat
     2701 gtggtctacc cggcccattg ccccggctgc ggctcggctg tcgccgatgc cggagcgctc
     2761 tgcggcccct gctggcagac catgccattc atcacaaggc cgttctgcga gcggctcggc
     2821 acgcccttcg cctatgatca gggcggatcg ttgctgtcac cggctgccat tgccgaccca
     2881 ccggttttcg accgcgcccg cgccgtcgcc cggcatgatg gccttgcccg ctcgatggtt
     2941 caccgactga agttctcaga ccggctagac cttgcccggc cgatgggagg gttgatggcg
     3001 gtggcgggca aggatgtgct ggcaggggcc gatgccctgg tcccggtgcc gctccattgg
     3061 acccgcctgc tctggcgccg cttcaaccag gctgaggtcc tggccgattg ggtgggcagg
     3121 gaatccggcc ttgccgtcct cgcccgaacg cttcgccgcc gcaagcacac gccgcagcag
     3181 gtcggactga gccgtcccca gcgcatcgcc aacctgcagg gcgcctttgt cgtacgcccc
     3241 gatcaggccg ccggcatcga gggcaagcat cttgtgctga tcgacgatgt gcataccacc
     3301 ggagccaccc tcaacgcctg cgcccgcgtg ctgcgcaggg ccggcgctgc tcgaatcgat
     3361 gttctgacct tcaccaaggt tgcagatgag gcatagccgc acctatctat gctgttgacg
     3421 atcacccggc aggctggccg aaaggagtgc catggtcacc cccgagataa ccatctacac
     3481 caagggctac tgccccttct gccaggacgc caaggcactg ctccagcgca agggggcggc
     3541 cttcaccgag atcgacgtga ctgccgatcg tgatcggcat gccgagatgg tgcagcgcgc
     3601 cggcggccgc cgcaccgttc cgcagatctt catcggcaca atccatgtcg ggggctgcga
     3661 cgacatctac gcgctggacg caaagggcgg cctcgactcc ctgctcaagg gttgaacgat
     3721 gatccgctat gcgcttgcct gcgaggccgg gcatggcttt gacagctggt tccgcagctc
     3781 ggaggatttc gacgctcagt gcgcgcgtgg gctcgtcgcc tgcccctatt gccactccac
     3841 caccatcgcc aagcaggtca tggcgccgat cgtggcgcgc agcgaccgcg gcgagccggc
     3901 cgcgcctgcc ccgctcgccc tgattgacga caaggccgcc gaactgcgcc gtagaatcgc
     3961 ggaactgcga acctatgttg ccgagaactg cgagaatgtc ggcccgcgtt ttgccgaaga
     4021 ggcccgcagg atccattatg gagagagcga aacccgctcg atctgcggcg aggcaagccg
     4081 cgaggatgcc gctgcgctga tcgacgaggg cgtgccgatc ctgccggtgc cggtcctgcc
     4141 ggatgaccga aattgacggc ggtcaggctg gcctggtcgc cgcgatgaag tagttgaccg
     4201 ccgtgtcgct ggacaggtgc catcgatcgg caaggggatt gtagaccatt ccccgcgttt
     4261 caacctcgcc cagaccggtg acggtcagcg cctcggcaaa ctcctccggc gtgacgaact
     4321 gctcccagcg atgcgtgccg accggcagcc agcgcagcac atattcggcg cccagaatgg
     4381 ccagcgcata ggcacgcttc gtccggttca gcgtcgatcc gagaaacagc ccgcccggtc
     4441 gcaccagcgc ggcggcggtg gcgacaaacc ccgccacatc cggcacatgc tcgaccactt
     4501 ccatcgccag caccacatcg aatgtgcgcc cgccagcgac cagatcctcg gcaagcccct
     4561 cgacatagct gaccgccaga ccttcgcctt cggcatgggc gcgcgcaacg gcaatattgt
     4621 tgggcgccgg atcgatgccg gtgaccgccg cgcccatccg cgccagaggc tcgcacagca
     4681 acccaccgcc gcagccgata tccaggatgc tgagccctct cagcgcatcg agcgcctggg
     4741 cgttgcggcc gaagcgggcc gcgattgccc ccctgatata ggacaggcgc accgggttga
     4801 tccggtgcag cggtttcatc gggccattct cgtcccacca atcggccccg atccggtcaa
     4861 attgcgacac ttcccgctca tcgacgctgt tgctggcccg cgcattcatg atttgtcctg
     4921 acatatcgtc gcaaggatgg ggtgttgagg cttctcaaaa ccgctttggc gctttatagc
     4981 gcggcacgaa ggcggcggca tccctgtttt accgcaaggg aaccgccgcg gacatcggcc
     5041 acatttgcaa ctctggaagc catgccccgc ctagtgatga aattcggcgg cacctcggtc
     5101 gccaacgtcg agcgcatcaa gaacgtcgcg cgccacgtca agcgcgaggt cgatgccggc
     5161 aatgatgtcg cggtcgtcgt ctcggccatg tcgggcaaga ccaacgagct ggttgcctgg
     5221 tgcaaggaga ccgacccgct ccatgaccag cgcgaatacg atacggttgt ggcctcgggc
     5281 gaacaggtca cctctggcct gctggccatc gcgcttcagg gccttggtct caaggcgcgc
     5341 tcgtggcagg gctggcagat cccgctttac accgacgctg cgcatggggc ggcgcgcatt
     5401 gccggaatcg acggttcggg cattctcgcc ggctttgcca agggcgaggt cgcggtgatt
     5461 tccggttttc agggcatcca caaggaaacc ggtcgcatcg ccacgctcgg ccgcggcggc
     5521 tccgacacct cggccgtggc ggtcgctgcc gggctcaagg ccgaccgttg cgacatctac
     5581 accgatgtcg acggcgttta caccaccgat ccgcgcatcg tcaaagccgc ccatcgcctc
     5641 gacaaggtct cgttcgagga gatgctggag atggcttcgc tgggcgccaa ggtgttgcag
     5701 gtgcgctcgg tcgagcttgc catggttcac aacgtgcgca cctatgtgcg ctccagtttc
     5761 gacgatccag ccaaccccca tccgggcacg ctgatctgta gcgaggagga gatcttggaa
     5821 cagcagatcg tcaccggcat cgcctattcc aaggacgagg cgcagatttc ggttcgcggc
     5881 gtcgccgacc gcccgggcat tgctgctgcg gtgttcgaac cgctcgccgc cggtcatatc
     5941 gtcgtcgaca tgatcgtcca gaacgtgtcc gaggacggca agtccaccga catcaccttc
     6001 accgtgccgt catcggatta tgaccgcgcg ctggccgttc tcggccaact gaagggcgag
     6061 atcggctatc gcgctctgca gggcgccaag gatgttgcca aggtctcggc catcggcatc
     6121 ggcatgcgct cgcattcggg tgttgccgcc aaggcattca aggcgctggc cgaccgcggc
     6181 atcaatctgc gcgccatcac cacctccgag atcaagttct cggtgctgat cgacgaggaa
     6241 ttcaccgaac tcgccgtccg caccctgcac catctctacg gcctcgacgc cgcctgaggc
     6301 ggcccgccct tggcccacga tctgccgaac gccctgcggc aggcgattgc cgaacacact
     6361 ggcagcctgg ctaatggact ggctgaagcg cgctcggaac tgacggccgg ctaccggtcc
     6421 ggcgctgcat cgcgcatcgc gatgacgagc cagcgccata tcgccgccta tctgatcgcc
     6481 cgcctgccgg caacctacgc ggctgtggcc gcagcgctgg agcggcttgc cggccaatgg
     6541 tcgggctttg cgccgaaaag ccttttcgat gccggctgcg gccccggaac cggactgttt
     6601 gccgctctgg tcgcctttcc ctcgctggac cagctggcgg ggcttgatcg cgatgcggcg
     6661 ctgattgaag tcgcccgcga tcttgtgacc cggtcaggtc tggccggcga tcggccggtc
     6721 accttcacga cggatgatct ggcacgtccc ggccttgccc ttgcgccggc agacctgatc
     6781 aattgcacct atgcgctggt tgaatgcgac gaggacgata tcggccggat tgccgcgacg
     6841 ctgtttgctg cagcgcgtca ggcgctggta ctggttgaac ctggaacgcc ggccggcttc
     6901 gaacggctca gggcagcgcg cacggccctg atcgggcaag gcgcgcagat cctcgcgccc
     6961 tgcccacacc agtctgcctg cccgatcacg gctcccgact ggtgccattt ctcggtgcgc
     7021 cttgcccgca gccgcgatca ccgccggctg aaagacgccg atgcgccctt cgaggatgag
     7081 aaattcagct atctgatcgc cgcgcgggca ccgggtgcca atcaaacggg cggtcgcatc
     7141 ctgcgtccgc cccggcacgg caaggccggc agccgctttg atctgtgcac ggatgccgga
     7201 aacatccaat cccgcactgt cccggcacgc gacaggaccc tgcaccgctc cgtgcgcgac
     7261 cttgtctggg gcgattttct gccggaagtg cctgactgag gcggacattc cactttttga
     7321 cctgcaacca ccgtcttgcg cacttggcgg ctggcatggc gctcgctata gggcgataag
     7381 gaactcagcg ccgccgcgat ccctcgcggc cggcccagcg ctggaggaga atgcggtcga
     7441 tgcgcggtgg tgtcggaccc agggtgctcc tgcgccgcct gcgggaggtg atggccgagc
     7501 ccgtcagccc gcaggaacgg ctggaccgca tcgtcgttct catcgctgcc aacatggtgg
     7561 ccgaggtctg ctcggtctat gtgctgcggg ccgatggcgc gcttgaactc tacgccaccg
     7621 aaggtctgaa acgcgaagcc gtgcatcaga cgaccatgcg cgccggtgag ggtcttgtcg
     7681 gcctgatcgc gcgtgaggcg cgggcgctgt cgctgtcgga tgcgcagagc catgcctcgt
     7741 tctcctaccg tccggaaacg ggtgaggaaa tctacgcttc gttcctcggc gttccgatcc
     7801 tgcgcgccgg cgacacgctc ggcgttctgg tggtccagaa ccgcgcgagc cgcagttatt
     7861 cggaggaaga ggtcgaggcg ctggaaaccg ccgccatggt catggcggag atgctggcga
     7921 cgggcgaact cgaagcgctc gcccggcctg gttccggcgg cattgccctc cgtcgcaccc
     7981 tggccctgca gggtcgcccg atcaacgacg ggatcggcct tggccatgcg gtgctgcacg
     8041 aaccgcgcgc ggtggtcacc aacatcgtcg ctgacgatgt cgaggccgaa gcacgccggc
     8101 tggacgaggc gatcgagacc atgcgcgcct cgatcgacga tctgatcgat cagggcaacg
     8161 tcagccatgc cggcgaacat cgcgatgtgc tcgagaccta tcgcatgttc gcctatgatc
     8221 gcggctggct gcggcggttg cgcgaggcgg tgatgagcgg cctgacggcg gaagctgcgg
     8281 tcgagcgcgt gcagtccgat gcgcgcgccc gcatgctgcg ccagaccgat ccctacctgc
     8341 gcgagcggct gcatgatctt gatgatcttg ccaaccggct gctgcaccaa ttgaccggct
     8401 cggccttcac gctgcatgcc tccgaattgc ccgagaacgc catcctcgtt gctcgctcaa
     8461 tgggcccggc ggcactgctg gaataccagc gctcgaaatt gcgcggtctg gtgcttgagg
     8521 agggcggcgc cacgtcccat gtcgccatcg tcgcccgcgc gctcggcgtg ccctgcctcg
     8581 gcgaggtcga ggggctcacc tcgctgatcc agccgggcga tgcgatcatc atcgacggca
     8641 cctcgggtga tgtgcatgtg cgcccgccac cggatcttgt cgccgcctat gccgagcggg
     8701 cgcggctgcg tgccggccgc atggaactct accgcaagga aaagaccctt ccctcggtca
     8761 gcaaggacgg gatcaaggtt gacctcttga tgaatgccgg tctgctggtt gacctgccca
     8821 accttgccga caccaacgcc gccggcattg gcctgttccg caccgaaatc ctgttcatgg
     8881 tcgcccccac cctgccgcgc gcctccacac aggaggggct gtatcggtcg atcctgacca
     8941 ttgccggcag ccggccggtc actttccgca cgctcgacat cggcggcgac aaggtgctgc
     9001 cctacctgcg caaggtggag gaggagaacc ctgccctggg ctggcgcgcg atccgaatcg
     9061 ggctggatcg gccagcgctg ctgcgcaccc agatgcgcgc gctgatcgcc gctgctgctg
     9121 accgtgatct caggatcatg ctgccgatgg tggccacccc ggccgagttc gacgccgccc
     9181 gcaccctgct tgaccgcgaa cgcgagcgca cgcgccgcct gggacggccg caaccgcgct
     9241 cgatcaagct cggcgccatg ctggaggtcc cctcgctttt gttcgacctc gatgcccttc
     9301 tccggcgcgt cgatttcatc tcgatcggct cgaatgatct gatgcagttc ctgttcgccg
     9361 tggatcgcga caatgcacgc gtcgccaccc gctttgatga tctcagcccg gccttcctgc
     9421 gtgccttgcg cctgattgcc gacaaggctg ttgcggccaa tgtgccggtg accctgtgcg
     9481 gtgagatggg cggacgcccg ctcggcgctt tggcgctgtt tgccctgggt ttccgctcgc
     9541 tgtcgatggc cccggcccgc atcggcccgg tcaagagcat gctgcgcgcg ctcaacatcg
     9601 gtgagaccag ccgctggctg gctgaaatcc tcgattcgga catttcgggc gaagcgctca
     9661 agcagcggct gacagcctat gcggaggcca acggcatccc gctttagcgc atcatcccgc
     9721 caggtggata ccggctggcg gaaaaagatg atgcgttcag tataggttag agcaacagag
     9781 cgatccagtc agatcggctt ttgctccaga gcatgctcca gccggctgcc acttgatcgc
     9841 ggcatgctct gaacgactgg atcagtcgac gacgccgaca aacggcaatt cgcgatagga
     9901 atgggcgaca tccatcccgt agccgaccag aaacaggtcg ggcgagacga agccgacgaa
     9961 atcggcgtcg atctgcaccg cccggtggcc cggcttttcc accagcaccg cgaccatcac
    10021 cttgcgggcg ccgcgcgcca tcaagaggtc cttggcaaag gccagcgtgc ggcccgattc
    10081 gaggatatcg tcgatgatca gcacatcgct accgcgcaca tcaccctcga tgtccttggt
    10141 gatggtgact tgcccggacg aaacggttcc ctcgcgatag ctcgaaagct gcatgaactc
    10201 gacctggggc gacatgcctt cccgatgcat cgcgcggaga aggtcggcgg cgaagatgaa
    10261 gctgccacgc aggaccgaga ttaccagcag ccgctcgggc ctggctgccg cgatcgcatg
    10321 ggcaagttcc ctgacccggc gcgcaatctc ggtctcgtca tacaataccc tgataggccg
    10381 ctcgatcatt ccgctcactc caactgtaag caccgtcata gaccagacgg aaggcaagag
    10441 caaaagcccg ggcaggtatc ggaaggggcc gtgatcgtca ggatcaggca agacctgcaa
    10501 agtcagtttg ccagcgccag aaggtcgtcc ttgctggcaa aacggaccag cacttcaacg
    10561 gcttcgggcg gcggcgaggc caaccggcgg cggaaacttt gcatggtgcc cggctgcaga
    10621 tccttggtgt cgggctgtgc cgtccagctg tagatttcat gccccttggc gtccatcagg
    10681 gcaaatttga gtcgcggcac cggccgcggc agcggcgaga cgttgacgat ctcgccttgc
    10741 acgaccagaa gctcgacatt gccctcctga accagcccgc ccttgatctg gcgcagggcc
    10801 aggccactga gattgaccgg cagaccgaag gccgcgtaga cacggccaag atcgggcagc
    10861 cgctccacga tgcttttgcg taacccgacc gccacgacca ggcaggccgc cagtgccgcc
    10921 acaatcacca acggacgcct gaatgagctg gtttggccgg cagggcggat ccgctggtgc
    10981 cagggcctcg ggcgctcgac gggcatcatt tgtggaacgg gggtcgcccc ctccgtctcc
    11041 tcgatcgctt cggccgccgg ttcggcaggc tcttccgatc cggccggccg cgccagccac
    11101 agggtgcggc aagcggtgca gcgcaccttg cgcccctccg gaccaaagct gctttcgggc
    11161 agttcatagc tcgctgcgca ggccggacac ttgattagca gcatggccat ccaggcttgg
    11221 gttgggaccc ggtcgcccac gcgggcaggc ggcgaatccg caacagttga cctcgctatt
    11281 gctgaaccgt catcgttaac ggcaggtaaa agggtgactg attcgcacgc tgcaaggagg
    11341 agcggtggtc cgcttcgaaa acgtcggcct gcggtatggc atgggccccg agatcctcaa
    11401 ggacctcagc tttgacgtgc cgccgcacac gctgcagttt ctcaccggcc cttccggcgc
    11461 cggcaagacg acattgctgc gcctgatcct gcagacgctg cgtccgacgc gcgggctcat
    11521 cagcctgttc ggccatgata cctccagcct cgacaaggat gcgatcaccc gtctgcgccg
    11581 ccgtatcggt gtcgtctttc aggatttccg cctgctcgat catctgacga cctatgagaa
    11641 tgtcgccctg cccctgcggg ttcagggtcg agacgagcgc atctaccggc ccgaagtgat
    11701 cgaactgctc aaatgggttg ggcttggcga tcggatgcat gtttccccgg ccatcctttc
    11761 cggtggcgaa aagcagcggg cggcgatcgc ccgcgccacc atctcgcgcc ccgaattgtt
    11821 gctggccgat gagccgaccg gcaatgtcga tccgtccctg gcccgccgcc tcttgcgcct
    11881 gttcatcgag atgaaccgct ccggcacgtc ggtggtgctg gcgacccatg atctggcttt
    11941 gatggatcag gtcgatgcgc ggcgcatggt tctggcggag gggcggctgc atgtctatga
    12001 ctgaagcgcc ggaagtcgtc acccgccccg cagggctcgc cgaacggctc ggccttggcg
    12061 ggcggcgcgg aacaccgttg ctgccggact cttcgctggc cggacgggcg ctgatcatcg
    12121 tcatcatcat catggcgttt ctcgcctcga tcactgccgg caccgtgcag ctgatcgctc
    12181 aggcctcatc cgactggaca ggcaccgtgg cccgcgaaat gaccgttcag gtgcgcccgc
    12241 gctctggccg cgatctggag gccgatgtgc gccgtgcggc cgacatcgcc ggccgtgttc
    12301 ctggcattac cgaagtccgg atcttcaccc gggccgaagc tgagcgcttg ctggaaccct
    12361 ggctgggtgc cgggctggcg cttggggaac tgccagtgcc gcggctcatc gtgctcaggc
    12421 tcgatccggc tggacaggcc gatgtcgccc gcctgaaagg cgatctcggc gccgtgccga
    12481 ccgccagcct cgacgatcat cgcctgtggc tcaagcgtct tgccaccatg gcgcggacgt
    12541 tggtggtgat cggcctcgtc gtcatggcgc tggttctggt cgcgatggcg ctggccgtgg
    12601 cctttgccac acgcggagcg atggccggca accgggaaat catcgatgtg ctgcatctgg
    12661 ttggtgccga agatcggttc atcgcgcgcg agttccagcg tcatttcctg aaactggggc
    12721 tgcgcggcgg cgcaatcggc ggcggacttg cggttctggc ctttctgctg gcatcgagtt
    12781 tgtcgcagcg ctggattgca acgccaggcg gcgaacaggt ggaagcgctg ttcggcagtt
    12841 ttgcccttgg ttttggcggt tacgcggccg tcgccgtcat tgccggcctt gtggcggcga
    12901 tcacggcggt ggtctcgcgc acaaccgtgt tccgcaacct gcgggggctc gattgaccga
    12961 cgatcacatg gacgccgcgc gccgaagcat ggcatgatcg agagcatcat cccgccaggt
    13021 ggaaaccggc tggcggaaaa agatgatgcg ttcagaattg attagagcca aagagcgatc
    13081 cagtcagatc ggcttttgct cttgagcgtt ttcaagcgaa gtggagtccg gttcgcgtga
    13141 agaaaatgcg atacaacaag aaacgaaagt actttcaatg aatgcgaatt cactgaaagt
    13201 gctctaacgc gtctgccggc cagaatccgg cgcgctccat ctgtacgatc ctggagttca
    13261 tgcagcgtgg catctgcccc tcttccgcca tccgatttgc ctccgggtct tgccccggat
    13321 gcaccggtcg agccggtggg ctggtcaagg cgacggcgtc ttgcggcggc gctgttggcc
    13381 ctgacgggcc tggcatctgt cgctgtatat ggcgggttcc tcgtgttcgt cagcagcctg
    13441 tcgcggcagg agacggaatt cggccggacg gtggacggca tttcggcctt gaccggcggc
    13501 gccgagcgca ttgtcgacgc gctggcggtg ttgagtgagg ggcgcgccaa gcgcctgctg
    13561 atttccggag tcaaccttca gaccagccgc gcctcgctga tccgtcagag cggccatgcc
    13621 gaactgttcg aatgctgcgt cgacatcggc cgggcggccc tgaacacggt cggcaatgcg
    13681 atcgagacgg cagaatgggc ccgccgcaac aaatatgcgt cggtgatgct ggtcacctcc
    13741 aattaccaca tgccgcgggc cctggtcgaa ctgcggcgga atctcaagaa tgtcgagatc
    13801 gtcgcccatc cggtcattac cgaacaggtg cgggtcgaaa cctggtggca cgatcccgcg
    13861 ctgttccggc tgctgttcca cgaatatctc aaatatctgt tttcggagat gcggtcgcgg
    13921 ctggtgccgg acaatcctcc cgccggacgc ggtgcttgac gcgggccgaa gaaccggctt
    13981 tgcctctgac cggcaatcca tgccccccgg gactccagat gcttctgctc cgcaacagtc
    14041 tttttgctgt cgccttttat gccaattgcg cgttctggtt tctgtttgcc tgcttcggct
    14101 atgtgattcc ggcccgtcat ttcggctttt tctgccgtgg ctgggcaacg acatcgatct
    14161 ggctgttccg caccatcatc ggcgcggaaa tcgaggtgcg cggccaacac aatatcccgc
    14221 atgaggcggc gctggtggcg gccaaacatc aatcctcctt cgaaacgatc ttcctgctga
    14281 tcgtgctcga ccggccgagc tacatctaca agcgggaact cggctatgtt ccgctgtttg
    14341 gctggcacct gctgcgcgcc ggacagattc cggtcgatcg tgacggtggc gccgaggcga
    14401 tggccagcct ggtcgcgcgc tcgcgccggg cgcttggcga agggcggcag atcatcatct
    14461 ttccggaagg aacgcgccgg gccgtcgatg caccgcccga ctacaagctc ggcgtggccc
    14521 agctttatcg cacgctcggg gttccctgcg tgccggtcgc gctcaattcg gggctggcct
    14581 ggccgcgccg cgccttcatg aaggaaccct accgggtggt cgtcgagttt cttgagccga
    14641 tcccggccgg cgttcccgcc aagaccgcct tccgtcgctt gcaggaagac atcgaaacgg
    14701 cgtcaaatcg attggtcgac gaggcgctgg ccgccgccgg tcagacgcgc gccgagatcc
    14761 agtccaagag tgaatcgtga atgccgagat ccttcagcgc cagtgcggta ttgcgcacat
    14821 actccgggtt gggacccgat tgaccaacgc cctgccggac gagccgcaga acatcctcgc
    14881 ggctgagacg cgcggcatat tggtgatgcc cgcgatcgac gacatagcac agtgccagaa
    14941 ccatccgccc gtcatcgagc gcaaccgggc ggacacattc cttgtagacc atattgacct
    15001 gttcgcgcgc ccgcagatag gcgatggttt cggccttctt gtgcgcatcg acacggaaca
    15061 cgacaccgcg acaggcaccg ccgtgatcga gcccgagcac cagccccggt tgatccctgg
    15121 tgccgcgatg cacatgcgaa tagacacaga gcgagcgatg tgcccccttg agcaacgccc
    15181 ggtgccgctc gacataatcg aaccccggcc gccagatcag cgatccgtag ccgaacaccc
    15241 agagatcgcc gtgttgatgc agcatggccg ctttctgtct tgaaattgga acggaaatca
    15301 aggcccaatc aggacaacac ggcaaggaga cagcatggac accgccgcgc cagccccgca
    15361 acgttcgcgc aagggattgt atattccgct gatcctgttc gccctggcct gcctgggttg
    15421 ggccgggttc tggtttttcg ccaaatcccg cgcccttgat gtcatggatg cctggctggc
    15481 cagggaggcg cggctggggc gggaatggac ctgcccccag cgcaccgctg gtggctttcc
    15541 cttccgtttc gaggtctcct gcaccggacc gaccttcgcc agcgctgaac ccggccgcgc
    15601 cggcaaggga tcgctcggcg cgttgacggt caccgcccgc gtcgtcgatc cgcgtcaggt
    15661 catcgccgcc gcgaccgggc cgctgaaatg gacctcggag gtcggcgatt cggtcgagat
    15721 cacctttgat ggcgcccgcg cctcctatcg cggcagcggg tccgcgctcg aggaactgtc
    15781 gatcgacctt gccaacccgc ttttcaccta cacggccccc ggcctgttca ccgagaaggc
    15841 ggcggccggc cgggccgaat tccacatgcg ccgcgccccg ggcgaggaag ctgcgaccga
    15901 ccttgtgctg attgcctccg caatcaaatc cgcccttgcc gaccagttga ccggcgagac
    15961 agccaacgga agaattgatc tgcgcacaag gctcaccaag ctgctgccat caccgccacg
    16021 caactggcgc gaaacgctgg aactgtggcg agtcgccgga ggcgaggccc gtgtcgagaa
    16081 actggccctt gccaagggcc ccatcgtgat cgaggcgacg ggagccttgc gcctggacga
    16141 gacccgccgg cttgatggcg agatcaccgg ctccgcacag ggcttgccgc agatcctgca
    16201 ggcgttcggc ctgggcggca atggcggcgg cctgctgggc tcgctcctgt caggcggtcg
    16261 ccctcaacag gcccagccgg ccaaggccct gccgttcgcg ctgcgctttg ccgaggggcg
    16321 gctgttcatc ggcccgattc cgggcccccg gctgcgtccg ctgttctgag cggaactcat
    16381 accaagagtc attgaaaatg actctttggt gcaactcaaa aatttcttat ctcattgata
    16441 atagtgagaa atttttgaaa cgaaccaagt cgc
//
LOCUS       DAOMXG010000036        16430 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_1991_length_16430_cov_10.554321, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000036 DAOMXG010000000
VERSION     DAOMXG010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 16430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16430
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1991_length_16430_cov_10.554321"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="PLQ11_04095"
     CDS             <1..485
                     /locus_tag="PLQ11_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HRK24117.1"
                     /translation="ARTATTPAVGGEPGNVFGTGKSVPRIAVAHRIPYVATATVADLH
                     DLERKVVKAMSIRGARYLQIDVPCPLGWGAASHDTIRLARLAVETGFYPIFEAEHGKV
                     TAVRKIRRKVPVTDYLKPQKRFAHLFKGGVEDARVARLQAMCDANIEEYGLLGDDSES
                     "
     gene            497..2122
                     /locus_tag="PLQ11_04100"
     CDS             497..2122
                     /locus_tag="PLQ11_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HRK24118.1"
                     /translation="MDHPFAITLDVGTSLNNQTGSWRNVRPEYVHRLPPCNHACPAGE
                     DIQGWLAHAEAGNYEAAWRSLTENNPLPAVMGRVCYHPCEGACNRKDIDSAVGINSVE
                     RFLGDTALAKGWAFEKPARESGKKVLVVGAGPSGLSAAYHLRRFGHQVTIREAGPLPG
                     GMMRFGIPKYRLPREVLDGEVKRITDMGVVIEYNAKVENIQKAMKQGKFDAAFLAVGA
                     HIAKRAYIPAGEAAKILDAVQVLRSMEGEDKPLLGRRVVVYGGGNTAIDVARTAKRLG
                     AEEAVIVYRRTRDKMPAHDFEVEEALEEGVMMKWLSTIKNVDAGSITVEKMALDDKGF
                     PQPTGEFETLEADSVVLALGQDVDLGLLDGVPGLEIADGVVKVGPNMMTGAAGIFAGG
                     DMVPSERTVTVAVGHGKKAARHINAWLNGESYDPAEKHEIASFDKLNPWYYGEAPHTV
                     RPVLEIARRTSTFEEVVKGLDESNALFEARRCLSCGNCFECDNCYGVCPDNAVIKLGP
                     GKGFEFNYDFCKGCALCAAECPCGAIRMVPEAI"
     gene            complement(2129..2347)
                     /locus_tag="PLQ11_04105"
     CDS             complement(2129..2347)
                     /locus_tag="PLQ11_04105"
                     /inference="COORDINATES: protein motif:HMM:NF024736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRK24119.1"
                     /translation="MEFTEGALIENRTRGGRRPLDHRRVLDGVLYVTRTGVPWRDLPP
                     EFGHWNSVHRQYRRWTEAGWREKMMRSE"
     gene            complement(2552..3019)
                     /locus_tag="PLQ11_04110"
     CDS             complement(2552..3019)
                     /locus_tag="PLQ11_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010970133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HRK24120.1"
                     /translation="MHATMDRTDLRILEILQADGRITNLQLAEQVGLSPTATSERMRA
                     LLREGYVTGYGARLDPQKLGFGLLVFIEVNLDKTTPDVFERFAAAARRVPQILECHMV
                     AGGFDYLIKARMKDMADYRAFLGDVMLSFPGVRETRSYAVMEEVKADAPLWLR"
     gene            3146..6856
                     /gene="putA"
                     /locus_tag="PLQ11_04115"
     CDS             3146..6856
                     /gene="putA"
                     /locus_tag="PLQ11_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012706686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HRK24121.1"
                     /translation="MSAASGPYLPQHDGAHSVFADFAPAVRPLTPLRQAIVARYRLAE
                     PDLLPGLIQAATLPEAVTAKAQSTARKLVEGLRAMPRGGGVEGLVQEFSLSSQEGVAL
                     MCLAEALLRIPDNETRDALIRDKIATGDWSSHLGTGKSLFVNAATWGLVVTGKLTATV
                     PEGGLAGALTRIIGRLGEPIIRRGVDLAMRLMGEQFVTGQTIEEALETARVREAQGFS
                     YSFDMLGEAATTAEDAKRYLEAYVGAVHAIGVASAGRGPYAGPGISVKLSALHPRYSR
                     SQAHRVMAELLPRLTHLARLAKSYDIGFNIDAEEADRLDLSLDLLQSLCEDHELAGWN
                     GLGFVVQAYGKRCPAVIDFLVDLARANKRRLMVRLVKGAYWDAEIKRAQVDGLSDYPV
                     FTRKIYTDVSYIACARKLLAAKDAVFAQFATHNAHSLATIHAMAGEDFKLGDFEFQCL
                     HGMGEPLYEQVVGTDKLNRPCRIYAPVGTHETLLAYLVRRLLENGANSSFVNRIGDPE
                     VPVDALIADPVAEARAMKTVGAPHKAILAPVNLFLNRANSRGLDPSNEVVLARLGEAM
                     AKRAKTRYAAGPMLAVPSTAKAKRPVRNPGDHRDEVGTVIDASEADVAAAVARAGAAF
                     RSWSDTDIADRATALEQAADRMESHYADLLPLIMREAGKTLANAIAEVRESVDFLRFY
                     GGFARSGLKPGASGLGPVACISPWNFPLAIFTGQVAAALVAGNPVLAKPAEETPLVAA
                     AAVRLLHESGVPKDVLQLLPGDGRIGAALVSQPGVHGVMFTGSTEVARRIQAVLSGRL
                     LSDHRSVPLIAETGGLNAMVVDSSALAEQVVQDVLASAFDSAGQRCSALRILCLQEEI
                     ADRTLTMLKGAMDELSIGRTDRLSIDIGPVITGEARSMIEAHVSAMAGRGFAVHRLHL
                     SQESAHGTYVAPTLIEVDSVRDVEREVFGPVLHVVRFARADLAKLIKDINASGYGLTF
                     GLHTRIDETIAEVRGSIKAGNIYVNRNIIGAVVGVQPFGGEGLSGTGPKAGGPLYLSR
                     LLDAPDQFDPGLPRKAGTEAAKVFASWLLSAGLHDLADRAEALAARARVGLEMDLPGP
                     VGEKNLYQLKPRGACLLSPVTKIGLYQQIATVLAAGCSGIIDMSDINPDFLLDLPVVV
                     TKRLRPAARAFSASVDAALVEGDANRAARYVDHFAKLPGRIVPVQTLSSEALEAGTRS
                     YAVEGLLEETSTSINTAAAGGNASLMTLDINP"
     gene            complement(6857..7489)
                     /locus_tag="PLQ11_04120"
     CDS             complement(6857..7489)
                     /locus_tag="PLQ11_04120"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HRK24122.1"
                     /translation="MTSPATWYFDVISPFAYLSLPGLRRLGAHRPIVMKPVLFAGLLN
                     HWGQLGPAEIAPKRRHIYRQTVFMAERLGLPCRLPPTHPFRPLAILRLLTALGASEAA
                     VTAAFRLVWGEGRDPNDPAVLADVAQEGNDPGAVERIDSPAIKQALADATAAAIAAGV
                     FGVPTLAIDDELFWGLDALPMAEAYLADPALFGTGEYARLDTIAASARRR"
     gene            complement(7486..8025)
                     /locus_tag="PLQ11_04125"
     CDS             complement(7486..8025)
                     /locus_tag="PLQ11_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010684778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HRK24123.1"
                     /translation="MPLIDRSGCRLILIDLQERLLPAIEGGAVAVENARRLAEAATRL
                     DVPIIRTEHYPKGLGTTVAPLAGYGRMIEKITFNSCGEPAFVEAVAGAASLVVAGCET
                     HVCLLQTVLGLLARGHRVHVVEDAAGSRTGESKRAALRRMRREGAEIVTTEMVIFEWL
                     VRGDHPAFKPVSALIRTLP"
     gene            complement(8046..8660)
                     /locus_tag="PLQ11_04130"
     CDS             complement(8046..8660)
                     /locus_tag="PLQ11_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015930476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRK24124.1"
                     /translation="MNDLAQIVDPRCVSARSTLFSQGEEAANVFNITEGMARLYKLLP
                     DGRRQIIGFALPGDFLGLSLSDQFSFSADAVNDMRICRIPRAKFAQQLDDKPRLLKRL
                     HEMAANELMIAQDQLVVLGRRSAEERVAVFLIGLRKRFTAIHHLSPTIPLPMSRQDIA
                     DYLGLTIETVSRTFSKFFREKTLLNVPDGVRILDLDRLNSLAMR"
     gene            8724..9341
                     /locus_tag="PLQ11_04135"
     CDS             8724..9341
                     /locus_tag="PLQ11_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24125.1"
                     /translation="MVTAVQAGILAMSGVRTGNRGAETFGDHGLFVQRTLMQVNDWFS
                     QPMKPMKIGGGRTGGRRRVEAMFEVRLIGREAVRQAFAVMLLGFPGLSFAEFRKLVAS
                     TRKGALSGLFDRRGYIHAVFRSRIELVPGGSRRLDVSDLVLSDSLSQAVASQMVESLL
                     RSARSEGCDQLTVQWPTGGKSAIIPQELLAKLGFYGESVLMARRI"
     gene            9515..11170
                     /gene="ccoN"
                     /locus_tag="PLQ11_04140"
     CDS             9515..11170
                     /gene="ccoN"
                     /locus_tag="PLQ11_04140"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HRK24126.1"
                     /translation="MAQTAPVQKSLKANEAALGGFFAVFALLWLYIASGSTEPEYTFH
                     AVLFVLGSLVACLAVFKRYAARTVQVEPQEINGLPNYNLGPIKVAAVMAMFWGIAGFL
                     VGDLAAWQLAVPALNFDLPWTNFGRLRPLHTSAVIFAFGGNVLLATSLYVVQRTCRAR
                     LAGDLAPWFVVLGYNFFILVAGTGYLLGITQGKEYAEPEWYADLWLTVVWVVYLLVFV
                     GTLIKRKEPHIYVANWFTLSMILTIAMLHLGNNLTVPVSIFSPRSVTVWSGVQDAMFQ
                     WWYGHNAVGFFLTAGFLAIMYYFVPKRANRPVYSYRLSIIHFWALVFLYIWAGPHHLH
                     YTALPDWAQTLGMTFSVILWMPSWGGMINGLMTLAGAWDKLRTDPVLRMMVVSVAFYG
                     MSTFEGPLMSIKAVNSLSHYTDWTVGHVHSGALGWVGYVSFGALYCLIPWLWNKRELY
                     SARLVNWHFWVSTIGIVLYVSAMWVSGIMQGLMWRAYTSLGYLEYSFVETVDAMNPYY
                     IIRAIGGLLFVAGSLIMAYNLWKTATSDALAIRQSGAAAPAAA"
     gene            11182..11937
                     /gene="ccoO"
                     /locus_tag="PLQ11_04145"
     CDS             11182..11937
                     /gene="ccoO"
                     /locus_tag="PLQ11_04145"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HRK24127.1"
                     /translation="MADTSNAPKSMWDKHKIFETNVVALILGCLVMVSIGGLVEIAPL
                     FYLKSTIEKVEGMRPYSPLELAGRRIYVREGCYNCHSQMIRPLRDEVERYGHYSLAAE
                     SMYDHPFQWGSKRTGPDLARVGGKYSDEWQRAHLAQPRAVVPQSIMPGYPFLEKTELN
                     LGNVVLEMKAQRMAGVPYSDEQMTNALADLKAQADPDDKGADAVAKRYPKAQVRNFDG
                     NAKLTEADALIAYLQVLGTLVDFKLYDEKANRR"
     gene            11948..12103
                     /locus_tag="PLQ11_04150"
     CDS             11948..12103
                     /locus_tag="PLQ11_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HRK24128.1"
                     /translation="MQSTYQFLAELAQSAGLVYFVTMFLGVCAYAMWPRNKARFEEAA
                     AIPLKED"
     gene            12108..12977
                     /gene="ccoP"
                     /locus_tag="PLQ11_04155"
     CDS             12108..12977
                     /gene="ccoP"
                     /locus_tag="PLQ11_04155"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HRK24129.1"
                     /translation="MATNIEKDAVTGTSTTGHEWDGIKELNTPLPKWWVNLFYVTILW
                     SIGYWIVYPAWPLVSGYTTGLFGYSTRGDVVEELNALKAKRGQNAAALEKASLDDIKK
                     NPALAQVANAIGKAAFGDNCVPCHGIGGAGAKGYPNLTDDDWIWGGDLAAIEQTVKHG
                     IRWATNKDTRVGEMMAFGKSGILKKDEVEQVVDFVRSLSKLDTKADVAKGKDLYAANC
                     ASCHGDDGKGNKDVGAPNLVDAIWLFGKDRDTMIDVVTNGRGAVMPAWTGRLDAVTIK
                     SLAVYVNGLGGGK"
     gene            12974..14431
                     /gene="ccoG"
                     /locus_tag="PLQ11_04160"
     CDS             12974..14431
                     /gene="ccoG"
                     /locus_tag="PLQ11_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HRK24130.1"
                     /translation="MTNTPHAVEEETFIEGPLYASAKKIYPQRVQGTYRQIKWALLFF
                     CLAVYYFLPFVRWDRGPNLPNQAVLLDLENRRFYFFFIEIWPQEVYYFTGLLIIAAMT
                     LFLLNSIAGRVWCGYLCWQTVWTDLFLAVERHIEGDRRDRIKLDSEPWHARKIAKKVS
                     KHAIWLAIAWWTGGAWVLYFADAPTLVNQLIRFEAPFLAYMWIGILTATTYVFAGIMR
                     EQTCLYMCPWPRIQAALTDDQALNVAYRYDRGEPRMSVKEAKRAVAAGDPAGDCVECL
                     QCINVCPTGVDIRHGSQLGCIQCGLCIDACDTVMKKLGRPTRLIAYDTDDAILKRSRG
                     ETPVYRYIRARTIIYLVMIGLIGGGMIYKLATRMTAGINVIHDRAPLFTTLADGSIRN
                     AYTVRILNKRPEVRDFALTVEGPLGATIEVVGVPATADWRPVVEVGPDRTREVRVLVS
                     VPKDQVKEKSVPITLRAADLFVGESVSAKDHFFGP"
     gene            14478..14999
                     /locus_tag="PLQ11_04165"
     CDS             14478..14999
                     /locus_tag="PLQ11_04165"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HRK24131.1"
                     /translation="MPVDLSPANQPQRKEWTLTGRFVLICLVTFFVVVAAVNAVMMTL
                     AIRTFPGIDARNGYEVSQRFNQDIAAARTQSERGWGAESMLERAGEGARVRLALTQRD
                     GRPVTGLAIEARLRHPSDRRLDQVLALSEAAPGQYEGVVTALSAGAWGLDFDGRTGGE
                     TVYRLVSRVTLKN"
     gene            15004..>16430
                     /locus_tag="PLQ11_04170"
     CDS             15004..>16430
                     /locus_tag="PLQ11_04170"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRK24132.1"
                     /translation="MSALPSATGRDLTLFVRSEQPGIDAMDLAVGGIKCAGCMSAIEK
                     GLAAEPGIVSARVNLASKRLTVEWQHGAMKPERIIDRLDEIGFRAHPFSARRLESEEA
                     REEKRLLRCLGVAAFGAMNIMLLSVSVWSGNFSDITPETRDFFHWLSALIALPIAAYS
                     GRPFFESAIRAIKAGAVNMDVPITLGIVLALGMSVVETLQSAEHAYFDSAVMLIFFLL
                     IGRFLDQSVRRRTRDVAANLATLRADTATRVAADGSLVDVPVEAIDPGDVVLAAPGQR
                     IAVDGLVESGESDVDQSLITGETRHVAVRPGANVYAGTLNVSGQLRIRVTKAESGTLL
                     AEIERLLSSAIEGRAKYVRLADRAARLYAPVVHATAALTFLGWLIAGMAWKPALLIAI
                     TVLIITCPCALALAIPAVQVVATQALFRRSILLHSPDALERLSEVDTVVFDKTGTLTL
                     PEPRLANADALPAEMLGAAARLAAA"
BASE COUNT         2909 a         5007 c         5415 g         3099 t
ORIGIN      
        1 cggcgcggac ggccaccacc ccggcggtcg ggggcgagcc gggcaatgtg ttcggcaccg
       61 gcaagtcggt gccgcgcatc gcggtggccc atcgcattcc ctatgtggca actgccaccg
      121 tggccgatct gcacgatctt gagcgcaagg tggtgaaggc gatgtcgatc cgcggcgcgc
      181 gctatctgca gatcgacgtg ccctgtccgc tcggctgggg ggcggcctcg catgatacca
      241 tccggctggc gcggctggca gtggagaccg gcttctatcc gatcttcgag gccgagcacg
      301 gcaaggtgac ggcggtgcgg aaaatccgcc gcaaggttcc ggtgaccgac tatctcaagc
      361 cgcagaaacg cttcgcccat ctgttcaagg gcggggtcga ggatgcgcgc gtcgcccggc
      421 tgcaggcgat gtgtgacgcc aatatcgagg agtatggtct cctcggcgat gacagcgaga
      481 gctgagggac aagaccatgg atcatccttt cgcgatcacg ctcgatgtcg gcacctcgct
      541 caacaaccag acggggtcct ggcgcaatgt gcggccggaa tatgtgcatc ggctgccccc
      601 ctgcaaccat gcctgcccgg ccggcgagga cattcagggc tggctcgcgc atgcagaagc
      661 aggcaattac gaggcggcct ggcggtcgct gaccgagaac aacccgctgc cggcggtgat
      721 ggggcgggtc tgctaccacc cgtgcgaagg cgcctgcaac cgcaaggaca ttgactcggc
      781 agtcggcatc aactcggtcg agcggtttct cggcgatacg gcgctcgcca agggctgggc
      841 atttgaaaag ccggcccggg agagcggcaa gaaggtgctg gtggtcggcg ccggcccctc
      901 cggcctgtcg gcggcgtatc atctcaggcg cttcggccat caggtgacga tccgcgaggc
      961 tgggccactg ccgggcggga tgatgcggtt cggcattccg aaataccgcc tgccgcgcga
     1021 ggtcctcgat ggcgaggtca agcgcatcac cgacatgggc gtggtcatcg aatacaatgc
     1081 caaggtcgag aatatccaga aggcgatgaa acagggcaag ttcgacgcgg ccttcctggc
     1141 cgtcggcgcc catatcgcca agcgcgccta tattccggcc ggagaggcgg cgaagatcct
     1201 cgatgcggtg caggtgctgc gctcgatgga gggcgaggac aagccgctgc tcggacgtcg
     1261 tgtggtggtc tacggtggtg gcaacaccgc gatcgacgtc gcccgcaccg ccaagcgtct
     1321 tggcgccgaa gaggcggtga tcgtctaccg ccgcacccgc gacaagatgc cggcgcatga
     1381 tttcgaggtg gaggaagcgc tcgaagaagg cgtgatgatg aagtggctgt ccaccatcaa
     1441 gaacgtcgat gcgggctcga tcaccgtcga gaagatggcg ctggacgaca agggctttcc
     1501 gcagccgacc ggcgaattcg agacgctgga agccgattcc gtcgtcctcg ctctcgggca
     1561 ggatgtcgat ctcggcctgc tggacggcgt gccggggctg gagatcgccg acggggtggt
     1621 gaaggttggc cccaacatga tgaccggcgc ggccggcatt tttgccggcg gtgacatggt
     1681 gccgtccgaa cgcaccgtca cggttgcggt cggccatggc aagaaggccg cacgccacat
     1741 caatgcctgg ctgaacggcg agagctatga cccggcagag aagcacgaga tcgccagttt
     1801 cgacaagcta aacccatggt attacggcga ggcgccgcat accgtccgtc cggtgctgga
     1861 gatcgcccgg cgcacctcca ccttcgagga ggtggtgaaa gggctcgacg aaagcaatgc
     1921 actgttcgag gcgcggcggt gcctctcctg cggcaattgc tttgaatgcg acaattgcta
     1981 cggtgtctgc ccggacaatg cagtaatcaa gctcggaccg ggcaagggct tcgagttcaa
     2041 ctacgatttc tgcaagggtt gcgcgctctg cgccgccgag tgcccatgcg gggcaattcg
     2101 catggtgccg gaggcgatct gacgcgacct attctgaacg catcatcttt tcccgccagc
     2161 cggcttccgt ccagcgtcga tattgccggt gcacagagtt ccagtggccg aactcgggcg
     2221 gcagatcacg ccacggaacg cccgtgcgcg tcacatacag aacgccatcg agcacccgcc
     2281 gatggtccag cggccgtcgc ccaccacgcg ttcggttctc gattagagca ccttcagtga
     2341 attccaattc acagaaagtg ctctatcttt ttgttttttc gcattttctt aacgcgaacc
     2401 ggaatccact tcgcttgaaa atgctctagg aagggcgcga aaaacgccca ctccacatcg
     2461 ctcatcaatc cacgaagcac cgctgccctc ctcgaagagc ggcgttgaat cagacatcag
     2521 atgatttggg aatcccgttt gtccatacga cctaacgcaa ccacagcggc gcatcagcct
     2581 tgacctcttc catcacggca tagctgcgcg tttcacgcac gcccgggaag gatagcatca
     2641 cgtcgccgag gaaggcgcga taatcggcca tgtccttcat ccgggccttg atgagatagt
     2701 cgaagccgcc ggcaaccatg tggcattcga gaatctgcgg cacccggcgg gcggcggcgg
     2761 caaagcgctc gaacacgtcc ggcgttgtct tgtcgaggtt gacctcgatg aaaaccagca
     2821 ggccgaagcc cagcttctgc ggatccagcc gcgcgccata accggtgaca tagccttcgc
     2881 gcagcaaagc gcgcatccgc tcgcttgtcg ccgtcggcga aaggccgacc tgttcggcga
     2941 gctgcaggtt ggtgatgcgc ccatcggcct gcaagatctc gagtatccta aggtctgtcc
     3001 ggtccattgt cgcatgaatc actatgagac gtagatattc agtgattatc actatcatag
     3061 catgaaattt gtggatatat ctaaagctca tcggcggtta gaatcgatct gcgttcaata
     3121 gccattgtcc gattgcgaga cggtcatgtc agctgccagc ggcccctacc tcccacagca
     3181 tgacggcgcc cattcggttt ttgccgattt tgctccggcc gtccggccat tgacgccgct
     3241 gcggcaggcg attgtcgccc gctatcggct ggcagagccg gatctgctgc ccgggctcat
     3301 ccaggcggcg acattgcccg aagcggtcac cgcaaaggcg cagtcgactg cccgcaagct
     3361 ggtcgagggg ctgcgggcga tgccacgcgg cggcggcgtc gaggggctgg tgcaggaatt
     3421 ttcgctgtcg agtcaggaag gtgtggcgct gatgtgcctt gccgaggcgc tgctgcgcat
     3481 tcctgacaac gagacgcgcg atgcgctgat ccgcgacaag atcgcgacgg gcgactggtc
     3541 ctcacatctg ggaaccggca aatccctgtt cgtcaatgca gcgacctggg ggctggtggt
     3601 cacgggcaag ctgaccgcca ccgtgccgga gggcgggctg gcaggggcgc tgacgcggat
     3661 catcggccgg ttgggcgagc cgatcatccg gcgcggcgtc gatctggcga tgcggctgat
     3721 gggcgagcag tttgtcaccg gccagaccat cgaagaggcg cttgagaccg cgcgggtgcg
     3781 ggaggcgcag ggcttttcct attccttcga catgctgggc gaggccgcga ccacggcaga
     3841 ggacgcaaag cgctatctgg aagcctatgt cggcgccgtg catgcgatcg gcgtggcgtc
     3901 ggcggggcgg gggccctatg ccggaccggg catttcagtg aaactctcgg cgctgcatcc
     3961 gcgctacagc cggagccagg cacaccgggt gatggccgag cttctgccgc ggctgacgca
     4021 tctggcccgc ctcgccaaat cctacgacat cggcttcaac atcgacgcgg aagaggcgga
     4081 ccggctcgac ctgtcgctcg atctgttgca gtcgctctgc gaggatcacg aactcgctgg
     4141 ctggaacgga cttggctttg tcgtgcaggc ctatggcaag cgctgcccgg ccgtgatcga
     4201 ttttctggtc gatcttgccc gggccaacaa gcgccggctg atggtgcgtc tggtcaaggg
     4261 cgcctattgg gatgccgaga tcaagcgcgc gcaggttgac gggctttccg actatccggt
     4321 gttcacccgc aagatctaca ccgatgtgtc ctacatcgcc tgcgcccgca agctgctggc
     4381 ggcgaaagac gcggtctttg cgcaatttgc cacccacaat gcccattcgc tggcgaccat
     4441 ccacgccatg gccggcgagg atttcaagct tggcgacttc gaattccagt gtctgcacgg
     4501 catgggtgag ccgctctacg aacaggttgt cggaaccgac aagctcaacc ggccctgccg
     4561 aatctatgcg ccggtcggca cccacgagac gctgttggcc tatctggtcc gccgactgct
     4621 ggagaatggc gccaacagtt cgttcgtcaa ccgcatcggc gatccggaag tgccggtcga
     4681 tgcgctgatt gccgatccgg tcgccgaagc cagggcgatg aagacggtcg gcgcgccaca
     4741 caaggccatc ctggcaccgg tgaacttgtt tctcaaccgc gccaattcgc gcggcctcga
     4801 tccgtcgaac gaggtggtgc tggccaggct cggcgaagcc atggcgaagc gggccaagac
     4861 ccgctatgcc gcaggcccga tgctggccgt gccgtcaacc gccaaggcca agcggccggt
     4921 gcgcaatccc ggcgaccacc gcgacgaagt gggcacggtg atcgacgcca gcgaggcgga
     4981 tgtcgcggca gccgtcgccc gtgccggcgc tgcattccgc agttggtcgg acaccgatat
     5041 cgctgatcgg gcaacggcgc tggagcaggc ggccgaccgg atggaaagtc attacgccga
     5101 tctgctgccg ctgatcatgc gcgaggccgg caagacgctg gcaaatgcca ttgccgaagt
     5161 gcgcgagtcg gtcgatttcc tgcggttcta tggcggattt gcccgcagcg gattgaagcc
     5221 tggcgccagc gggctggggc cggtcgcctg catcagccca tggaattttc cgctggcgat
     5281 cttcaccggg caggtggcgg cggcgctggt ggcgggcaat ccggtgctgg ccaagccggc
     5341 agaggagacg ccgctggtcg ccgcagcggc tgtgcgcctg ctgcacgaga gcggcgtgcc
     5401 gaaggatgtc ctgcagctgc tgcccggtga tggccggatc ggcgccgcat tggtaagcca
     5461 gcccggcgtg catggcgtga tgttcaccgg ctcgaccgag gtggcacgcc gtatccaggc
     5521 ggtgctgtcc ggcaggctgc tgtccgatca ccggtccgtg cctttgatcg cggagaccgg
     5581 cgggctcaat gccatggtgg tcgattcctc cgcactggcc gaacaggtgg ttcaggatgt
     5641 gctggcctcg gccttcgaca gcgccggcca gcgctgctcg gcgctgcgca ttctctgtct
     5701 ccaggaggag atcgccgatc gcacgctgac gatgctcaaa ggagcgatgg atgaactcag
     5761 catcggccgc accgaccggc tgtcgattga tatcggcccg gtgatcaccg gcgaagcacg
     5821 gtcgatgatc gaggcgcatg tttctgccat ggccgggcgc gggtttgccg tgcatcgcct
     5881 gcatctgtcg caggaatccg cgcacgggac ctatgtcgcg cccacgctca tcgaggtcga
     5941 tagcgtcagg gatgtcgagc gcgaagtgtt cggcccggtt ctgcatgtgg tccgtttcgc
     6001 ccgggcggat ctcgccaagc tgatcaagga catcaacgcg tccggctatg ggctgacctt
     6061 cgggctgcac acccggatcg acgagacgat cgccgaggtg cggggcagca tcaaggcagg
     6121 caacatctat gtgaaccgca acatcatcgg cgccgtcgtg ggcgtgcagc cgttcggcgg
     6181 cgagggatta tccggcaccg gccccaaggc tggcgggccg ctgtatctgt cacgactgtt
     6241 ggatgcgccg gaccagttcg atcccggcct gccacgcaag gcggggaccg aggccgccaa
     6301 ggtattcgcc agctggctgt tgtctgccgg cctgcacgac ctggccgacc gtgccgaagc
     6361 gctcgccgcc cgcgcccgcg tcggcctgga gatggatctg ccgggaccgg tgggcgagaa
     6421 gaacctctac cagctcaagc cacgcggcgc ctgcctgctg tccccggtca ccaagatcgg
     6481 cctctaccag cagatcgcca cggttctggc ggcaggttgc agcggcatca tcgacatgtc
     6541 cgatatcaac cccgatttcc tgctcgacct gcctgtggtc gtgaccaagc gcctcaggcc
     6601 cgcagcgcgg gccttttcgg ccagcgtgga tgctgctctg gtggaggggg atgctaaccg
     6661 cgctgcccgc tatgtcgatc atttcgccaa attgccgggt cggatcgtgc cggtgcagac
     6721 gctgtccagc gaggctctgg aagccggcac gcgcagctat gcggttgagg ggttgctgga
     6781 agaaacctcc accagcatca atacggcggc tgcgggcggc aatgcctcgc tgatgacgct
     6841 ggacatcaac ccctgatcag cgccgccggg cgctggcggc aatcgtgtca agacgcgcat
     6901 attcgcccgt cccgaacagc gccggatcgg cgagataggc ttcggccatc ggcagggcat
     6961 ccagccccca gaacaattcg tcatcgatgg ccagggtggg aacgccgaac acgcctgcgg
     7021 cgatggcggc ggctgtcgca tcggcaaggg cctgcttgat cgccgggctg tcgatccgct
     7081 cgaccgcgcc cggatcgttg ccctcctgcg cgacatcggc cagcacagcc ggatcgttgg
     7141 ggtcgcggcc ctcgccccag acaaggcgga atgccgccgt aaccgcggct tcggacgcgc
     7201 caagggcagt cagaaggcgc aggatggcga gcggacggaa cggatgcgtc ggcggcaatc
     7261 tgcagggcag accaagccgc tcggccatga aaacggtctg gcgatagatg tggcgccgct
     7321 tgggggcgat ctccgccggg cccagctggc cccaatggtt gagcagcccg gcgaaaagga
     7381 ccggcttcat cacgatggga cgatgggcgc cgagccgtct cagcccaggc agggacagat
     7441 aggcaaaggg cgaaatgacg tcgaaatacc aggtggcggg actggtcacg gcagggtccg
     7501 gatgagagcg gaaacaggtt tgaatgcggg gtgatcacca cgaaccagcc attcgaagat
     7561 caccatctcg gtggtgacga tctcggctcc ctcgcgccgc atgcggcgaa gcgcggcgcg
     7621 cttgctctcg cctgtgcgcg agccggcggc atcctccacg acatggacac gatggccgcg
     7681 cgccagcagg ccgagcacag tctggagcag gcagacatgg gtttcacagc cggcgacgac
     7741 cagcgaggcc gctccggcca ccgcctcgac gaaggccggc tcgccgcaac tgttgaaggt
     7801 gatcttctcg atcatccgcc cataaccggc gagcggcgcg accgtggtgc ccagcccctt
     7861 cggataatgt tcggtgcgaa tgatcggcac gtcgagccgg gttgccgcct cggccagtct
     7921 gcgggcgttt tcgaccgcga cagcgccgcc ctcgatcgcc ggcagtagcc tctcctgcag
     7981 gtcgatcagg atcaggcgac agccggaacg gtcaatcagc ggcatcggca ccccgcggga
     8041 tctgttcagc gcatggccag agaattcagc cggtcaagat caaggatgcg cacgccgtcg
     8101 gggacattga gcagcgtctt ttcgcggaag aacttcgaga aggtgcggct gaccgtctcg
     8161 atggtcaggc cgagatagtc ggcgatatcc tgccggctca tcggcagcgg gatggtcggc
     8221 gacaggtggt gaatcgcggt gaagcgcttg cgcagaccga tcaggaacac agccacgcgc
     8281 tcctcggcgc tgcggcggcc aagcacaacc agctggtcct gcgcgatcat cagctcgttc
     8341 gcggccatct cgtgcagacg cttgagcaga cgaggcttat catcaagctg ctgggcgaat
     8401 ttggcacgcg ggatccggca gattcgcatg tcgttgacgg catcggccga aaacgagaac
     8461 tgatcggaca ggctgagccc gagaaaatcc ccgggcagcg caaagccgat gatctggcga
     8521 cgcccatcgg gcagcagctt gtaaagccgt gccatccctt cggtgatgtt gaagacgttg
     8581 gcggcttcct cgccctgcga gaacagggtg gaccgggcag acacgcagcg cggatcgaca
     8641 atctgggcaa gatcgttcaa ttccgcctcg tccagcgcgc tgcagacgct gagaaaccgc
     8701 gtgcggcaat gcaggcagtc aacatggtga ccgctgtcca ggcagggatt ctggcgatgt
     8761 ccggcgttcg gaccgggaat cggggggcgg agaccttcgg tgaccatggc ctatttgtgc
     8821 agcgcacttt gatgcaggtc aatgattggt tttcgcaacc tatgaaaccc atgaaaattg
     8881 ggggtggtcg gaccggtggg agacggcggg tggaagcgat gttcgaagtc cgcttgatcg
     8941 ggcgcgaggc cgttcgccag gccttcgctg tgatgttgct cggctttccc gggctgagct
     9001 tcgccgagtt ccggaaactc gttgcttcga cacggaaagg tgcgctcagc ggcctttttg
     9061 accgtcgcgg ctatattcac gctgtttttc gcagtcgcat cgagctggtg cccgggggca
     9121 gccgtcggct ggacgtcagc gacctggtgt tgtcagacag cctgtcgcag gcggtggctt
     9181 cacaaatggt cgaatccctg ttgcgttcgg cccgctccga aggttgcgac caattgaccg
     9241 tgcaatggcc gacagggggt aaatcggcga tcattccgca ggaattgctc gctaaactgg
     9301 gcttttatgg cgaatccgtg ctgatggcgc gccgaatcta gcgccaatcg atgcccgggg
     9361 ggcagccgca gcggtgcact tccattctcg ccaaatggcc caccgtctca tttctgcttg
     9421 atttaggtca acgacccggt gagatggccg gagtcacaag ccactaaggt gactcaggct
     9481 gcgcacgcgg cctaaacctc aattggggga cgctatggcc caaaccgcgc cagttcaaaa
     9541 atcactgaag gccaacgagg ccgccttggg cggattcttc gcggtctttg ccctgctttg
     9601 gctctatatc gcttccggct ccaccgagcc ggaatataca ttccatgccg ttctgttcgt
     9661 gctcggcagc ctcgtggcct gccttgcagt gttcaagcgc tatgcggcgc gaacggtgca
     9721 ggtggagccg caggaaatca acggcttgcc caactataac ctcgggccga tcaaggtcgc
     9781 ggcggttatg gcgatgttct ggggcatcgc tggcttcctc gtcggcgacc tcgccgcgtg
     9841 gcagctcgcg gttccggcgc tcaatttcga tctgccgtgg accaacttcg ggcgcctgcg
     9901 tccgttgcac acctcggcgg tcatcttcgc ctttggcggc aacgtgctgc tggcgacgtc
     9961 gctctatgtg gtgcagcgca cctgccgggc gcggctcgcc ggtgacctgg cgccgtggtt
    10021 tgtggtcctt ggctacaact tcttcatcct ggttgccggc acgggctatc tgctcggcat
    10081 cacccagggc aaggaatatg ccgagccgga atggtatgcc gacctgtggc tgacggtggt
    10141 ctgggtcgtc tatctgctgg tgttcgtcgg cacgctgatc aagcgcaagg agccgcatat
    10201 ctatgtggcg aactggttca cgctgtcgat gatcctgacg atcgccatgc tgcaccttgg
    10261 caacaacctg acggtgccgg tgtcgatctt ctcgccgcgc tcggtgaccg tgtggtccgg
    10321 tgtgcaggat gcgatgttcc agtggtggta tggccataac gcggtgggct tcttcctgac
    10381 cgccggcttc ctcgccatca tgtactactt cgtgcccaag cgcgccaacc ggccggttta
    10441 ttcctatcgg ttgtcgatca tccacttctg ggcgctggtc ttcctctaca tctgggcggg
    10501 cccgcaccat ctgcattata ccgcgctgcc ggactgggcg cagacgctgg gcatgacctt
    10561 ctcggtcatc ctgtggatgc cctcgtgggg tggcatgatc aacggcctga tgacgctggc
    10621 cggcgcctgg gacaagctca ggaccgaccc ggtgctgcgc atgatggtcg tctcggtcgc
    10681 cttctacggc atgtcgacct tcgaaggccc gctgatgtcg atcaaggcgg tcaactcgct
    10741 cagccactac accgactgga ccgttggtca cgtgcattcg ggtgcgctgg gctgggtcgg
    10801 ctacgtgtcg ttcggcgcgc tctactgcct catcccctgg ctgtggaaca agcgtgagct
    10861 ttactcggcg cgtctggtca actggcactt ctgggtgtcc accatcggca tcgtcctcta
    10921 cgtgtcggcg atgtgggtgt cgggcatcat gcagggcctg atgtggcgtg cctatacctc
    10981 gctcggctat ctcgaatact cgttcgtcga gacagtcgat gcgatgaacc cctactacat
    11041 catccgcgcc atcggcggcc tgctcttcgt ggctggcagc ctgatcatgg cctacaacct
    11101 gtggaagacc gctacgtctg acgccctggc aatccgccag tccggcgcgg cggctccggc
    11161 tgcggcgtaa ggagaagcac aatggctgat acttccaacg ctccgaagtc catgtgggac
    11221 aagcacaaga tcttcgagac caatgtggtc gcgctgatcc ttggctgtct ggtgatggtg
    11281 tcaatcggcg gtctcgtcga gatcgccccg ctgttctacc tcaagtcgac catcgagaag
    11341 gtggagggga tgcgtcccta ttcaccgctc gaactggcgg gacgccgtat ctatgtgcgc
    11401 gaaggctgct acaactgcca tagccagatg atccggccgc tgcgcgacga ggtcgagcgc
    11461 tatggccact acagcctcgc cgcggaatcg atgtacgatc atcctttcca gtggggctcc
    11521 aagcgcaccg ggccggatct ggcccgcgtc ggcggcaagt attccgacga gtggcagcgg
    11581 gcgcatctgg cccagccgcg cgcggtggtg ccgcagtcga tcatgcctgg ctaccctttc
    11641 ctcgaaaaga cggagctgaa tctcggcaat gtcgtgcttg agatgaaggc gcagcgcatg
    11701 gcaggtgtgc cgtattccga cgagcagatg accaacgcgc tcgccgatct gaaggcacag
    11761 gccgatccgg acgacaaggg cgccgatgcg gttgccaagc gttatccgaa ggcgcaggtg
    11821 cgcaacttcg acggcaacgc caagctcacc gaggccgatg cgctgatcgc gtatctgcag
    11881 gtgctcggca ctctcgtcga cttcaagctc tatgacgaaa aagccaaccg gcgctgaggg
    11941 ggacgagatg caatcgacgt atcaattcct cgcagaactc gcccagtcgg cagggctggt
    12001 ctacttcgtg accatgttcc tgggcgtctg tgcctatgcc atgtggccgc gcaacaaggc
    12061 gcgctttgaa gaagcagcgg cgatcccgct gaaggaggac tgatcccatg gccacgaaca
    12121 tcgaaaaaga cgcggtcact ggcacttcga cgaccggcca cgagtgggat ggcatcaagg
    12181 agctcaacac tccgctgccg aaatggtggg tgaacctgtt ctatgtcacc atcctgtggt
    12241 ccatcggcta ctggatcgtc tatccggcct ggccgctggt ctccggctat acgacgggcc
    12301 tgttcggcta ttcgacccgc ggcgacgttg ttgaggagct caatgccctc aaggccaagc
    12361 gcggccagaa tgctgccgct ctggaaaaag cctcgctcga cgacatcaag aagaacccgg
    12421 cgctggcgca ggtcgccaat gcgatcggca aggcggcctt tggtgacaat tgcgtgccct
    12481 gccatggcat cggcggcgcc ggcgcgaagg gctatccgaa cctgaccgac gacgactgga
    12541 tctggggcgg cgatcttgct gcgatcgagc agaccgtgaa gcacggaatc cgctgggcga
    12601 ccaacaagga tacccgcgtc ggcgaaatga tggcgttcgg caagagcggc atcctgaaga
    12661 aggacgaggt tgagcaggtg gtcgatttcg tccgctcgct gtcgaagctc gacacaaagg
    12721 cggatgtggc caagggcaag gacctctatg cggccaactg cgcctcgtgc catggcgatg
    12781 acggtaaggg caacaaggat gtcggtgcgc ccaatctggt tgacgcgatc tggcttttcg
    12841 gcaaggaccg tgacaccatg atcgacgtgg tgaccaatgg tcgcggcgct gtcatgccgg
    12901 cctggacggg ccgtcttgat gcggtgacga tcaagtcgct cgctgtctat gtcaacggcc
    12961 ttggaggcgg taagtgacga ataccccgca tgcggttgag gaagagacct tcatcgaagg
    13021 accactctac gcctcggcga agaaaatcta tcctcaacgc gtgcagggga cctaccgcca
    13081 gatcaaatgg gcgctgctgt tcttttgcct ggcggtctac tatttcctgc ccttcgtcag
    13141 gtgggatcgt gggcctaacc tgcccaatca ggcggttctt ctcgatctgg agaaccgccg
    13201 cttctatttc ttcttcatcg agatctggcc gcaggaagtc tattatttca ccgggctgct
    13261 gatcatcgcc gcgatgacgc tgttcctctt gaattcgatc gccgggcgtg tctggtgcgg
    13321 ctatctctgc tggcagacag tctggactga tctgttcctt gcggtcgagc ggcatatcga
    13381 aggtgaccgc cgcgaccgga tcaagctcga ttccgagccg tggcatgcca ggaagatcgc
    13441 caagaaggtc tccaagcatg cgatctggct ggcaattgcc tggtggaccg gtggtgcctg
    13501 ggttctgtat tttgccgatg caccgacgct ggtgaaccag ctcatccgtt tcgaagcgcc
    13561 gtttctggcc tatatgtgga tcggcattct gacggcgacg acctatgttt tcgccgggat
    13621 catgcgggag cagacctgcc tttatatgtg tccctggccc cgcatccagg cggcgctgac
    13681 cgatgaccag gccctcaatg tcgcctatcg ctatgatcgg ggcgagccgc gcatgtcggt
    13741 caaggaggcc aagcgggcgg tggctgcggg cgatccggcg ggcgattgcg tcgaatgcct
    13801 gcaatgcatc aacgtctgtc cgacgggggt cgacattcgc cacggctcgc agcttggctg
    13861 catccagtgc ggcctgtgca tcgacgcctg cgatacggtg atgaaaaagc ttgggcgccc
    13921 gacgcggctc atcgcctatg atacggatga cgcgatcctc aagcggtcaa ggggggagac
    13981 tcccgtctac agatacatcc gggcgcggac gatcatctat ctggtcatga tcggactgat
    14041 cggtggcggc atgatctaca agctggcaac gcgcatgacc gccggcatca atgtcatcca
    14101 cgatcgtgcg ccgctgttta ccacgctggc ggatggcagt atccgcaatg cctatacggt
    14161 gcgcatcctc aacaaacgac cggaagtgcg cgactttgcc ttgaccgtcg aggggccgct
    14221 tggcgccacg atcgaggtgg tcggtgtgcc ggcgacggcg gactggcgtc ctgtcgttga
    14281 ggtcgggccg gatcgcacac gcgaggtgcg ggtgctggtg agtgtgccca aggatcaggt
    14341 caaggaaaag tctgtcccga tcacgctgcg ggcggcagac ctgtttgttg gtgaatcggt
    14401 gagcgcgaaa gaccatttct tcgggccgta gagcagatta ggatgtgatc atgacgtcct
    14461 gttgtggagt tgagctgatg cctgtcgatc tgagccctgc caaccagccg cagcgcaagg
    14521 aatggaccct gaccggtcgg ttcgtgctga tctgtctggt gacgttcttc gtggttgtcg
    14581 ccgctgtcaa cgcagtgatg atgacgctgg cgatccgcac gttcccgggg attgatgcgc
    14641 gcaacggtta tgaggtgagc cagcgcttca accaggatat tgctgcggcg cggacccagt
    14701 cggagcgcgg gtggggtgct gagagcatgc tggagcgggc tggcgaaggg gcgcgtgtgc
    14761 gccttgcctt gacccagcgt gacggccggc cggtcacggg actggcgatc gaggcacgcc
    14821 tgagacatcc gtcggaccgc cggctggacc aggtcctggc tttgtccgaa gctgcgcccg
    14881 gccagtatga gggcgttgtg acggcgcttt cggccggcgc ctgggggctc gacttcgacg
    14941 gcaggaccgg cggcgagact gtctatcgtc tggtcagccg cgtcacgctc aagaactgat
    15001 gccatgtcag cgctgccgtc cgcgaccggt cgcgatctga cgctgttcgt ccgttcggag
    15061 cagccgggaa tcgacgccat ggatctggcg gtcggcggca tcaaatgcgc cggctgcatg
    15121 agcgccatcg aaaaggggct ggcggccgaa ccggggatcg tttcagcgcg cgtcaacctc
    15181 gcctccaagc ggctgacagt cgaatggcag cacggggcga tgaagcccga acggatcatc
    15241 gacaggctcg acgagatcgg cttccgtgcc catcctttct cggcccgcag gctggagagc
    15301 gaggaagcgc gcgaggaaaa gcgactgttg cgctgcctcg gcgtggcggc cttcggcgcc
    15361 atgaacatca tgctgctgtc ggtatcggta tggtccggca acttttccga catcactccg
    15421 gaaacgcgcg acttcttcca ttggttgtcg gcgctgatcg cgctgccgat cgctgcctat
    15481 tccggccggc cattttttga aagcgccatc cgggcgatca aggccggtgc ggtcaacatg
    15541 gatgtgccga tcacgcttgg catcgttttg gccctgggca tgtcggtggt cgagaccttg
    15601 cagagcgcgg aacatgcgta tttcgacagc gcggtcatgc tgatcttctt cctgttgatc
    15661 gggcggtttc tcgatcagtc ggtgcgtcgg cggacacgcg atgtcgccgc caatcttgcc
    15721 accttgcggg ccgatacggc gacgcgggtt gcggctgacg gctcgctggt ggatgtaccg
    15781 gtcgaggcaa ttgatccggg tgacgtggtg ctggccgcgc ccggacaaag gatcgcggtc
    15841 gatggcctgg tagaaagcgg cgagtccgac gtcgaccaga gcctgatcac cggcgagacg
    15901 cggcatgttg ccgtccggcc cggcgccaat gtctatgccg gcacactcaa tgtctccggc
    15961 cagttgcgga tccgggtcac caaggccgag agcggcacct tgctggccga gatcgagcgg
    16021 ctgctgtcga gcgctatcga gggccgggcg aaatatgtgc gtctcgccga tcgggcggcg
    16081 cggctttatg cgccggtcgt gcatgccact gcggcgctga ccttcctcgg ttggttgatc
    16141 gccggaatgg cctggaagcc ggcgctcctg atcgccatca cggtgctgat catcacctgc
    16201 ccctgcgcgc tggcgctggc gatccctgcc gtgcaggtgg tggcgacgca ggcgctgttc
    16261 cggcgctcga tcctgttgca ctcgcccgat gcgctggagc ggctgagcga agtggacacg
    16321 gtggttttcg acaagaccgg gaccctgaca ttgccggagc cgcgactggc caatgccgat
    16381 gcgctgccgg ccgaaatgct gggtgcggcg gcgcggctgg cggcggcaag
//
LOCUS       DAOMXG010000037        16290 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_2009_length_16290_cov_9.741731, whole genome shotgun sequence.
ACCESSION   DAOMXG010000037 DAOMXG010000000
VERSION     DAOMXG010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 16290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16290
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2009_length_16290_cov_9.741731"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="PLQ11_04175"
     CDS             <1..337
                     /locus_tag="PLQ11_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRK24133.1"
                     /translation="LRDLLLARAGLAEARLSIPATVFATGHDLAVAVRTGEIDCGLAT
                     RAAAAANGLGFVPLATEHFDLVMRRRTYFEPGPQKVFAAMRSPEFQARAALFQGYDLA
                     DAGAIRFNG"
     gene            334..522
                     /locus_tag="PLQ11_04180"
     CDS             334..522
                     /locus_tag="PLQ11_04180"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24134.1"
                     /translation="MSHGRHPDFRAAPTDTLDGTADASSVVPPAAALGRVLAESVISP
                     YPVPARSIALADGYAVKH"
     gene            complement(956..2398)
                     /locus_tag="PLQ11_04185"
     CDS             complement(956..2398)
                     /locus_tag="PLQ11_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010658048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRK24135.1"
                     /translation="MGIFQNFIGGEGVGATSASRNINPSNTHDVIGEFASATVADVDQ
                     AVAAAKAAFPAWSRTTPQERHDILKKASDEVLARKDELGRILSREEGKTLPEGIGEAT
                     RAGQILAFFAGEALRLNGEAVPSIRPGVGIEITREAVGVVGMITPWNFPIAIPAWKIA
                     PALAYGNTIVLKPADLVPHSAWALCDILNRAGLPKGVLNLVMGKGSVAGQAIIDHKDV
                     SALSFTGSVATGRRIAAAAVGSQPMKKIQLEMGGKNPLVVLDDADLKTAVECAANGAF
                     FSTGQRCTASSRLIVTEGIHDAFVAALTERMQGLTVDDALKAGTHIGPVVDAKQLETD
                     ESYIAIGRKEGAKLHWGGERLNRENPGFYLQPALFTECTNQMRISREEIFGPVASVIR
                     VKGYDEALAVANDTEFGLSSGICTTSLKYATHFRRNSEAGMVMVNLPTAGVDYHVPFG
                     GRKGSSYGPREQGAYAREFYTTVKTAYTLA"
     gene            complement(2451..3673)
                     /locus_tag="PLQ11_04190"
                     /pseudo
     CDS             complement(2451..3673)
                     /locus_tag="PLQ11_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611527.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3818..4243)
                     /locus_tag="PLQ11_04195"
     CDS             complement(3818..4243)
                     /locus_tag="PLQ11_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRK24136.1"
                     /translation="MILLDTNVVSEAMKPQPDLSVRDWLDAQVAETLFLSSVTVAELL
                     FGIGALPDGKRKDNLTAALHGVMDLFGSRILPFDASAARRYADIAVKARMAGKGFPTP
                     DGYITAIAAAHGFAVASRDTGAFHAAGLTVINPWTDVQG"
     gene            complement(4243..4494)
                     /locus_tag="PLQ11_04200"
     CDS             complement(4243..4494)
                     /locus_tag="PLQ11_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HRK24137.1"
                     /translation="MAAVTIRNLSDETHRALKVRAAQHNRSAEAEIRAILEAAVRPES
                     RLRLSSTLSQMSRKIGLTNADVEALEQTRNANPAEPMRF"
     gene            complement(4874..4948)
                     /locus_tag="PLQ11_04205"
     tRNA            complement(4874..4948)
                     /locus_tag="PLQ11_04205"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4914..4916),aa:Thr,seq:ggt)
     gene            complement(5016..6293)
                     /gene="hemA"
                     /locus_tag="PLQ11_04210"
     CDS             complement(5016..6293)
                     /gene="hemA"
                     /locus_tag="PLQ11_04210"
                     /EC_number="2.3.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009494515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003870 - 5-aminolevulinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-aminolevulinate synthase"
                     /protein_id="HRK24138.1"
                     /translation="MYDYQATFKAARAALKAEKRYRVFTEIERDADRFPIARWHGPNG
                     LVRDVTVWCSNDYLGMGRHKDVVASMVGTASTCGVGAGGTRNISGNSHAVIELEKELA
                     ALHGKEAALVFSSGYVANQTAISTIAQLLPDCLILSDSDNHNSMIDGVRRAGRARAIF
                     RHNDAAHLEELLAAADPDRPKLIVFESVYSMDGDVSPIHAFCDLAEKYGAMTYIDEVH
                     AVGMYGAHGAGYAEEIGAMDRLDIIQGTLAKGYGCFGGYITGTEAMIDAVRSHAQGFI
                     FTTALPPPVAAAARAAIHHLRISQVEREAQRRQVRRVKQLLAEASIPVLDNPTHIVPV
                     MVGDAALAKLASDRLLDRHGIYVQPINYPTVPRGTERLRITPTPFHDDALIDQLIAAL
                     GEVWEALDLPRTAKVTKPAASTASITVFSPAGG"
     gene            6727..8016
                     /gene="murA"
                     /locus_tag="PLQ11_04215"
     CDS             6727..8016
                     /gene="murA"
                     /locus_tag="PLQ11_04215"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020092503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRK24139.1"
                     /translation="MDSIKITGGNRLNGTIPISGAKNAALPLMIAGLLTDRQLVLENV
                     PRLSDVRALARILGNHGVDITIAGKRRGEAQDGGDTLMINAATIVDTCAPYDLVSTMR
                     ASSWVVGPLVARMGECRVSLPGGCAIGTRPVDLHIMALERLGATIEIEEGYMVARAPK
                     GLKGAEIFFPSVTVGGTHVALMAATLAHGTTIIHNAAREPEVTDLADCLNQMGAKIRG
                     QGTSTIEIKGVSRLNGATHRVIPDRIEAGTYAMAAAMAGGDVLLEGARPEHLQAALDV
                     LRKAGVDITSSNEGIRVRRNGSGIDAVDVTTAPFPAFPTDLQAQLMALMTRARGTSHI
                     TETIFENRFMHVSELARLGARITLKGDLAIVEGVEKLKGAPVMATDLRASVSLVIAGL
                     AAEGVTTVNRVYHLDRGFEALEKKLSTCGAQIERIRA"
     gene            8018..9313
                     /gene="hisD"
                     /locus_tag="PLQ11_04220"
     CDS             8018..9313
                     /gene="hisD"
                     /locus_tag="PLQ11_04220"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HRK24140.1"
                     /translation="MRRIDQRSAGFAEAFATLIGMKREVSEDVDASVRAILADVRERG
                     DSAVADYTLRFDRVDLNATGFAFTRADIAAAHNRASPEAVGALRLAAERIRAYHERQR
                     PADARFTDGLGVSLGWRWTAVDSAGLYVPGGTASYPSSVLMNAVPARVAGVRRLVMVV
                     PTPGGVVDPLVLVAADIAGIDTVYRIGGAQAIAALAYGTGTIAPVAKIVGPGNAYVAA
                     AKRQVFGTVGIDMIAGPSEVVILADATGNPEWIAADLLAQAEHDTAAQSILITTDTAL
                     AEATSAAVERQLASLPRAAIAGASWRDFGAIITVGSLDEALPLIERLAPEHLELHLSD
                     AEAVAGRIRHAGAIFIGAHTPEAIGDYVGGSNHVLPTARSARFASGLGVMDFVKRTSL
                     LACSPEALRQLGPAAMVLGESEGLQAHARSVAIRLDDID"
     gene            9324..9815
                     /locus_tag="PLQ11_04225"
     CDS             9324..9815
                     /locus_tag="PLQ11_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0262 family protein"
                     /protein_id="HRK24141.1"
                     /translation="MSDDDPRKRLYSVVLDETSIERGTPDQEHERQVAIYDLIEENYF
                     ALAHDDGGPYALHLGLVSNRLVFDIRREQGDTVVVHVLSLTPLKKIVKDYFLICDSYY
                     EAIRNATADRIEAIDMARRGVHNEGSAIVRERLEGKIELDHQTARRLFTLISALSWTG
                     REA"
     gene            9812..10264
                     /locus_tag="PLQ11_04230"
     CDS             9812..10264
                     /locus_tag="PLQ11_04230"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HRK24142.1"
                     /translation="MSGPAAPIHSILFLCTLNSVRSPMAEGLARRKLGQYVHVESAGL
                     IRSERNPFALAVLREIGIDFSEDVPHAMNALAMEGFDLVVTLSDQAQAAVIDRLRFAS
                     IRHIHWSIHDPTEAGGSREARLQAFRSVRDDLRQRIRRELLPLLEKSD"
     gene            complement(10377..10453)
                     /locus_tag="PLQ11_04235"
     tRNA            complement(10377..10453)
                     /locus_tag="PLQ11_04235"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10417..10419),aa:His,seq:gtg)
     gene            complement(10608..11066)
                     /locus_tag="PLQ11_04240"
     CDS             complement(10608..11066)
                     /locus_tag="PLQ11_04240"
                     /inference="COORDINATES: protein motif:HMM:NF020918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HRK24143.1"
                     /translation="MAAAKKTSPVESVETAVQAALAPISQMQEKMRETAEKSLEQARS
                     QFEAVKDAAEKASGKLEESVNAAKSGVVAFNLKAIELARKNANVSFDHVQALFGAKTL
                     QDVFALQSEFVKKQLEVGQAQVQELAALGQKLAADAAEPVKSALSNSFKP"
     gene            complement(11243..11722)
                     /locus_tag="PLQ11_04245"
     CDS             complement(11243..11722)
                     /locus_tag="PLQ11_04245"
                     /inference="COORDINATES: protein motif:HMM:NF020918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HRK24144.1"
                     /translation="MLKNPFEVPNEMRDLASRSVEQARKAFETFIGAAQKASDQVHAG
                     PIPVPKNFADASRMTVEFAERNMNAAFDMAQKLVQAKTIEEALRIQTEYMQSQAHALQ
                     SQMQEAGNRVKLQMEDAASHMASTVETVKATVAKATNAATSAATAAVTPKAPKKPAK"
     gene            11967..14825
                     /locus_tag="PLQ11_04250"
     CDS             11967..14825
                     /locus_tag="PLQ11_04250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRK24145.1"
                     /translation="MAVLADHGILAALVGAARGAALERDERATVVFDETGAPRWASPS
                     ARSLFGSGMDGAVAFSAAAPGAQRLKAFIRAADSDRLAEPLRFFIGGRLEPLPVQALR
                     LTLADGTPGLVALFGEGPALNLPDPEPKKASEPASAVQSPLPQPAVEQDGARKTQRFV
                     FLTDAEGAISSVSPVFTQALGIHARDLVGRNFVDVIAEIDPVRAGDVARAMLAATTWN
                     GICVDWPMADGTRTLPVEFSAMPAFDASRALTGFNGFGLIRFERPANSATASPAVPAP
                     EPALPVSETAPATTDNDFEAAIRRLEAILGDVPHHQQDARPTPDASSGTAAGKHLAPR
                     PEGRPLPSPAPPADDASVVPLPSLKTHVGATLGEPKVVPLKPNLPPASGAQNAGLSHS
                     ERNAFREIAKALGARIETTPVGGHGKGKAAPLTPQTQTEVPPNRVAEAQAELSKALGV
                     PPAMAAGAQQPSAGFTDLLLERMPLGVLVFRGDKPLYLNRTLLDLTGYDTLAEFDALK
                     GLEKLFRGRAAPGLVTGEFDTVTIMGRDGATLPVDAHLQSIDWQGEHATLVTIRRAQD
                     AEDGPRAKALDLELKKRQAELGEAQAMLDTATDGVVSLDDGGRILALNKSAEALFGYD
                     QNELTGERLTLLLAPESHAAALDYLDGLKSGGVASVLNDGREVIGRERKGGRIPLFMT
                     IGRISDTGKFAAVVRDMTAWKRAEAELTEAKRAAERSSALKSDFLAKVSHEIRTPMNA
                     IIGFAEVMRDERLGPIGTERYKDYLNDIHTSGTHVISLVNDLLDLSKIEAGRMELTFA
                     SVDLNAIVTSCLGIMQPQANRDRVIMRSQLSAKLPPVVADERSLRQIVLNLLSNASKF
                     TEPGGQVIVSTALTDQGEAVIRVRDTGVGMTEQEIQAALEPFRQLSNARGRGGTGLGL
                     PLTKALVEANRASFVINSKVREGTLVEITFPPTRVLAE"
     gene            complement(14964..>16290)
                     /locus_tag="PLQ11_04255"
     CDS             complement(14964..>16290)
                     /locus_tag="PLQ11_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRK24146.1"
                     /translation="ADPNAPAAAPAEPKKKIVKRPPPKERGIVTDDPLPALVADSIRL
                     IQEAAQRYRQIAETGGWPSLPKQRLALGSKGPAVVALRQRLAAEGDLDAGATSQPVFD
                     KALQDALKQFQSRHGLARSGVVAGSTLEALNVSAATRARQLENSAQRLVDRNLNLSGR
                     YVVVNIPSASVEAVENGSVRRRYVAVVGKPENASPQVDARIGTVNINPTWTVPTSIIK
                     NEFIPKMRSNPANNFLARSNIRILDRSGNTVPASSIDWSTDRAVNYTFRQDSGSTNSL
                     GQIRIDMPNRDAVYMHDTPSKRFFNAQDRFFSHGCVRVQDVKGLAAWLLEGTAGNWDK
                     STIEAAISEGERKDIRLARPVPVAWVYLTGYVTDDGKTHFRDDVYSLDTPQAMASLVA
                     RRTQRQAQREAQQQNADFTASVPSAGSLATGQMIDPPVSDAPRRGWLWW"
BASE COUNT         2897 a         5227 c         5190 g         2976 t
ORIGIN      
        1 gcttcgggat ctcctgctcg cccgcgcagg ccttgcggag gcgcggctga gcattcccgc
       61 gacggttttc gcgacgggcc acgatcttgc cgtcgccgtg cgcacgggtg agattgattg
      121 tgggctggcc acgcgagcgg cggcggcggc gaacgggctc ggcttcgtgc cgctggcgac
      181 cgagcatttc gatctcgtga tgcgcaggcg cacctacttc gagcccggtc cgcagaaggt
      241 gttcgccgca atgaggagcc cggaattcca ggcgcgggca gcgctgttcc aaggctatga
      301 tctggcggac gctggcgcga tccgtttcaa tggctgagcc atggccgcca tcccgatttt
      361 cgcgctgctc caaccgatac tctcgacggt acagccgacg cctccagcgt cgtcccgccc
      421 gcagcggcgc ttgggcgtgt gctcgccgaa tcggtgattt ctccctaccc ggttccagcc
      481 cgttcgatcg cccttgccga tggctatgcc gtgaagcact gaacggcctg tcgactgcag
      541 cgttgctggt tcagccccgc tccatggctc aatccgtttt catgcacagg tcagattcga
      601 tcagctacga cagcccagcg gtgcagtatc aggctccaaa gcggtatcct gggggccggg
      661 ctgaacccag catcagcgac tggatcctcc atctggagcc cgccaaggtg cgcagatcgc
      721 gcggatactt cgccatcgct caggttcagc cgatcatccg tgaagctgga cgtctcagta
      781 tcggccacag tccttgaatc cccccgagcc aatgatccag tgctgttcag gttgaacgtg
      841 acttggaccg gcgcacgaga ttgaaccgca gacggaggat cgcgagaggc ggcgaaacaa
      901 aaaaaggcgg gccaagagag acccgccttg gaacatgctg gcgctgacgt cgtggtcagg
      961 cgagcgtgta agccgtcttc accgtggtgt agaactcgcg agcataggcg ccctgttcgc
     1021 gcgggccata gctggagccc tttcggccac cgaagggcac atgataatca acgcccgccg
     1081 tcggcagatt caccatcacc atgcccgctt cgctgttccg acggaaatgc gtcgcatatt
     1141 tcaggctggt ggtgcagatg cccgaggaga gcccgaactc ggtatcgttc gccaccgcga
     1201 gcgcctcgtc atagcccttg acccggatga cgctggcaac aggaccgaag atttcctcgc
     1261 ggctgatccg catctggttg gtgcattcgg tgaagagcgc gggttggaga taaaagccgg
     1321 gattctcccg gttcagccgc tcgccacccc aatgcagctt cgcgccctcc ttgcggccta
     1381 tggcgatgta gctctcgtcg gtttcgagct gcttggcatc gacgaccggg ccgatatgcg
     1441 tgcccgcctt caaggcgtca tcaactgtga ggccttgcat ccgttcggtc agcgctgcga
     1501 cgaaggcatc gtggatgccc tccgtcacga tcagccgcga ggaggcggtg cagcgctggc
     1561 ccgtggagaa gaacgcccca ttcgccgcgc attccacggc cgtctttagg tcggcgtcat
     1621 ccagcaccac gagcggattc ttgccaccca tttcgagctg gatcttcttc atcggctggc
     1681 tgccgacagc cgccgccgcg atgcgccgcc cggtggccac cgagcccgtg aacgaaagcg
     1741 ccgatacgtc cttgtggtcg atgatcgcct gcccggcaac cgaccccttt cccatgacaa
     1801 ggttgagcac acccttgggc agcccggcgc ggttgaggat atcgcacagt gcccaggcgg
     1861 aatgcggcac gaggtctgcg ggcttcaaca caatcgtatt gccataggcc agcgcgggcg
     1921 cgattttcca ggccggaatg gcgatgggga agttccacgg cgtgatcatg cccacgacgc
     1981 ccaccgcttc gcgggtgatc tcaatgccaa ctccgggccg gatcgaggga acagcctcgc
     2041 cattgagacg caaggcttcg ccggcaaaga aggcgagaat ctgcccggcg cgggtggctt
     2101 cgccaatgcc ttccggcaag gtcttgccct cttcccggct gagaatccgg ccaagctcat
     2161 ccttgcgggc cagcacctca tcggaagcct ttttcaggat gtcatggcgc tcctgcggcg
     2221 tcgtgcggct ccatgccgga aaggcggcct ttgccgcggc gacagcctga tccacatccg
     2281 cgaccgtggc gctcgcaaac tcgccgatga cgtcatgggt gttggaggga ttgatgttcc
     2341 gggatgccga agtggcgccc acgccttcac cgccgatgaa attctggaag atacccatgc
     2401 gaatgttcct ttcttgaagt gcgcgatgcg ccgttcggcg gcgcctgtga tcaaccctcg
     2461 gcgaggataa gatcggcggc catttccgca atcatgaaaa cagcggcctg cgtgttgccg
     2521 gagaccatcg tcggcatcac cgaggcgtcg gccaccctga gccccttcag cccatggacc
     2581 cgcaaggccg ggtcgaccac cgcttccggg ccgtggccca tgcgcttttc ggcgagccac
     2641 ttcgccaggt cctcgctgac gtaggaactg ccgttgtcgc tgagcagccg gggccgatgc
     2701 acgaccgtga cgtggtcgag ccctgatgcc ttcagcgcca ggtcaagcgt gtcggtgaca
     2761 tcgtcggcgc gcatcgtgct acacagcttc caggcgacaa tgtagcgcga gaagtcgtcg
     2821 agcaccgtcg agagatagta ccagccccag ccggtgatct tcaggtaggt gaagtcggtc
     2881 tgccagagct ggttaggcgc ggtggtcttg tccttgaact cctcggccgc cttgatcacc
     2941 acattggctg gcgaggtgat caggtcatgc gccttcagca agcgatagac cgatgcttct
     3001 gagacgaagt acctccgctc gtcgatgaag cgcacggcca gctcgcgcgg agacagctcc
     3061 ggcgcctcca gcgtaaggtc gacgatctgg gcccgaacca cgtcggggat acggttccag
     3121 acgcgatccg gccgtgagcg gcgatcggcc agcgcctcca cgccgccggt cagatagcgc
     3181 tcgtaccagc ggtagaatgt agcgcgcggg ataccgagct tgcgaagcgt cgccttggcc
     3241 ggcaggtgcg agccctcgac caggccgatg atctcggcct tctcggatgc ggggtatctc
     3301 attcgccgtc ctccccatcc ccggtcatgc tttttttgag caggcggttc tcgagcgtca
     3361 gatcggccac gacctccttg agcgcctggg cctcgcggcg caggcccttc acctcgtcgg
     3421 atgtggcggc ccgcgccgtg tcgcccgcca gacgccgctt gccggcctcc aggaactcct
     3481 tcgaccagcc gtaatactgc gaggcggcga tcccctcgcg ccggcacaac tccgagatgc
     3541 tgtcctcgcc ccgaaggcct tccagcacga tcctgatctt gtcctcagcc gtccaatgcc
     3601 gccgcgtcgc gcgccggata tccttgatcg cctcctccgc cgaactggcg ggcatcgtct
     3661 gcttctgtct catcgtcgct cctcttggtg acgatggaac agaaactctc ctttgccgag
     3721 atcaaccgac ctgtctcatg ggtgctgacg gggaacatcc gcgtcccatg atcgttctcc
     3781 ttcatcgccg agacattcgg cgaagagaac gaaggactca cccttgcaca tccgtccagg
     3841 gattgatcac ggtcagacct gcagcgtgaa atgcgcccgt gtcgcgtgac gcaacggcga
     3901 atccatgcgc agcagcgatc gcagtgatgt agccgtcagg cgtggggaaa cccttgccgg
     3961 ccatgcgcgc ctttacagcg atatccgcat aacgccgtgc cgcactggca tcgaagggca
     4021 ggatgcgcga cccgaacagg tccatgaccc cgtggagcgc cgcagtaaga ttgtcctttc
     4081 gcttgccatc cggcaacgcc ccgatgccga aaagcaattc cgctacggtg acactcgaca
     4141 ggaacagcgt ctcggctacc tgcgcgtcga gccaatcgcg gacagagaga tcaggttgag
     4201 gcttcattgc ctcggagacg acattagtgt cgagcaggat catcagaagc gcatcggttc
     4261 ggcagggttg gcattgcgtg tctgttcgag cgcctcgaca tcagcattgg tgaggccgat
     4321 cttcctgctc atctgcgaca aggtcgaact aaggcgaaga cggctttcgg ggcggacagc
     4381 ggcttccaaa atcgcgcgta tctcggcttc ggcgctgcgg ttatgctgcg ctgcgcgaac
     4441 tttcagagcc cggtgcgtct catcagaaag attgcgaatg gtgacagcag ccatgatagc
     4501 gcactccgta aaacgattgc gacgctatca ttatatgcat gacgatatca ttcttcaagc
     4561 gcgacgtcga acgcaccttc tcaatgaccg ctcacggacg aaactgcttg gctcctgaac
     4621 gaccgcgatg agggcgcatt cctgacgttc gaaccaatct gtgcggaaac tcgaaccgct
     4681 ctccggcttg cgcccaggcc gcactcggaa aatgcccaca tgggtgcgga gaaatgccct
     4741 gaaataagcc cccaaaagcg cccaaaattc tctgttgtca ggtctctctg aacgagggtc
     4801 gtttgcctaa gccccggaaa tagccagcct tctctacctg aaccggaaaa tcatcggttt
     4861 gcatgagagt ggctggtgct gccagagagg atcgaactct cgacctctcc cttaccaagg
     4921 gagtgctcta ccactgagct acggcagcga tgcggggcga cgtctgccac aggcgcccgc
     4981 ctgtggcaag cggcaaaacc ccagaagatg tcagatcagc cgccggcggg ggaaaacacc
     5041 gtgatcgatg cggtggaagc ggcgggcttg gtcactttgg cggtgcgcgg cagatcaagc
     5101 gcctcccaga cctcgcccag cgccgcgatc agctgatcga tcaacgcgtc atcgtgaaat
     5161 ggcgtgggcg tgatgcgcag ccgttcggtt ccgcgtggca cggtggggta gttgatcggc
     5221 tgcacgtaga tgccgtgccg gtcgagcagg cgatcggagg ccagcttggc aagggccgcg
     5281 tcgcccacca tcaccgggac gatgtgggtg ggattgtcca gcaccgggat cgaagcctcg
     5341 gctagcaatt gcttgacacg gcgcacctga cggcgctggg cctcgcgctc gacctgcgaa
     5401 atccgcaggt gatggatcgc cgcacgcgca gcggccgcta cgggcggagg cagcgcagtg
     5461 gtaaatatga acccctgagc atggctgcgc accgcatcga tcatcgcttc cgtgccggtg
     5521 atatagccgc cgaaacagcc gtaacccttg gccagggtgc cctgaatgat atcgagccgg
     5581 tccatggcgc cgatctcttc ggcatagcca gccccgtgag cgccatacat gccgacggca
     5641 tgcacctcgt cgatataggt catggcgccg tatttctcgg ccagatcaca gaaggcgtgg
     5701 atcggcgaaa catcgccatc catggaatac acgctttcaa agacgatcag cttgggccgg
     5761 tcaggatcgg ctgcggccag cagttcttca agatgagccg cgtcattgtg gcggaagatc
     5821 gcccgcgccc ggccggcccg cctgacgccg tcgatcatcg aattgtggtt gtcgctgtcc
     5881 gacaggatga ggcaatcggg gagcaattgg gcgatcgttg aaatcgccgt ctggttggcg
     5941 acatagcccg acgagaaaac cagcgccgct tccttgccat gcagggctgc gagttccttc
     6001 tcaagctcga tcactgcgtg gctgttgcca gagatgttgc gggttccgcc ggcgccgacg
     6061 ccgcaggtgc tggcggtgcc gaccatggac gccaccacat ccttgtgacg gcccatgcca
     6121 agataatcgt tggaacacca aaccgtgaca tcccgcacca gcccgttcgg gccatgccat
     6181 cgggcgatcg gaaaacgatc ggcatcacgc tcgatttcgg taaagacgcg ataccgcttt
     6241 tccgccttca gtgctgcacg cgccgccttg aacgtcgcct ggtaatcata catcggccta
     6301 caactcccga ccaaccccct cagggccggc atgccaattc atagcttgct ccaggcccgc
     6361 aaggccagcc cttttcaggc cgcttccggt cagggaccca actatggtaa gcgcaggcgt
     6421 cccatgcttt ccggccattc gtcattgcca tgaatcaaaa aggcccacca cagcccgtcc
     6481 ccactccgcc ttccgccgct gaagatcgcc gggcgcggct ttcggcggcc ttgcgggaaa
     6541 acctcaagcg gcgcaaggcg caggggcgcg cccgcaaggc gcaagagact ggcaagggcg
     6601 aaccgccaca ttcctcagga aactgacagc ggctaccgct tttttgctgt ctttggctga
     6661 gtttgtgcta tgcgcggggc gcattggcgg gcggggcggc tctggcaggt gcttaggaac
     6721 tgatcgatgg acagcatcaa gattaccggc gggaacaggc tgaacggcac gattccgatt
     6781 tccggcgcca agaatgccgc attgccgctg atgattgccg gccttttgac cgatcgtcag
     6841 ctggtgctcg aaaatgtgcc gcgcctttcc gacgtacgtg cactggcccg catcctcggc
     6901 aatcatggcg tcgacatcac catcgccggc aagcgtcggg gtgaggcgca ggatggcggc
     6961 gacaccctga tgatcaatgc ggcgaccatc gtcgatacct gcgcgcccta cgatctggtc
     7021 tccacgatgc gggcctcgtc ctgggtcgtc ggtccgctgg tggcgcgcat gggcgaatgt
     7081 cgggtgtccc tgccgggcgg ctgcgccatc ggcacgcgcc cggtcgatct gcacatcatg
     7141 gcgctggagc ggttgggtgc gacgatcgag atcgaagaag gctatatggt cgcccgcgcg
     7201 ccaaaagggc tgaagggcgc cgagattttc ttcccctcgg tgacggtcgg cggaacacat
     7261 gtggcgctga tggcggcgac gctggcgcat ggcacgacca tcatccacaa tgccgcacgc
     7321 gagccggaag tgaccgatct ggccgattgc ctcaaccaga tgggcgccaa gatcaggggc
     7381 cagggaacct cgaccatcga gatcaagggc gtctcccgcc tcaacggtgc aacccatcgg
     7441 gtgattccgg accggatcga agccggaacc tatgccatgg cggcagcgat ggcaggcggc
     7501 gacgtgctgc tggaaggcgc ccggcccgag catcttcagg cggcgctcga tgttctgcgc
     7561 aaggcagggg tggacatcac ctcttccaac gagggcattc gcgtgcgccg taacggcagc
     7621 ggcatcgacg cggtggatgt gaccacggca ccatttccgg ctttccccac cgatctgcag
     7681 gcgcagctga tggcgctgat gacacgcgcc cgcggaacgt ctcacattac cgagacgatt
     7741 ttcgaaaatc gcttcatgca tgtctccgaa cttgcgcggc tgggcgccag gatcacgctc
     7801 aagggcgatc tggcgattgt cgagggggta gaaaagctca aaggcgcccc ggtgatggcc
     7861 actgacctca gggcctccgt cagccttgtc atcgccggac ttgctgccga aggcgttaca
     7921 accgtcaacc gggtctacca tctcgatcgc ggcttcgagg cgctggagaa gaagctcagc
     7981 acctgtggcg cccagatcga gcgtatcagg gcctgacatg cgccgcattg accaaagatc
     8041 cgccggtttt gccgaagctt tcgccaccct gatcggcatg aagcgcgagg tttcggaaga
     8101 tgtcgatgcc agcgtgcgcg cgatcctggc cgatgtccgg gagcgcggcg atagcgcggt
     8161 tgctgactac acgctgcgct tcgacagggt cgatctcaac gcaaccggat ttgccttcac
     8221 gcgggctgac attgccgctg cccacaacag ggcaagcccc gaggcagtgg gagcgctgcg
     8281 cctcgccgcc gaacggattc gtgcctacca cgagcgtcag cggccggccg atgcccgctt
     8341 taccgatggc ctcggcgtgt cgctcggctg gcgctggaca gcggtcgatt ctgccgggct
     8401 ctacgtgccg ggcggcacgg ccagctatcc ttcgtccgtg ctgatgaatg ccgttccggc
     8461 ccgggtcgcc ggcgtcaggc gcctggtgat ggtggtgccg acaccgggcg gcgtcgttga
     8521 tccgctggtg ctggtcgccg ccgatattgc cggcatcgac accgtctacc gcatcggcgg
     8581 cgcgcaggcg atcgcggcgc ttgcctatgg caccgggacc atcgcccccg tcgccaagat
     8641 cgtcgggccg ggcaatgcct atgtggctgc cgccaaacgg caggtgttcg gcacggtcgg
     8701 catcgacatg atcgccggcc cgtcggaagt ggtcattctg gccgatgcga ccggcaatcc
     8761 cgaatggatc gcggccgacc tgctggcgca ggccgaacat gacaccgccg cccagtcgat
     8821 cctgatcacc accgatacgg cgctggccga ggcgacgtcc gcggcggtcg aacggcaact
     8881 ggccagcctg ccgcgggcgg cgatcgccgg ggccagctgg cgcgatttcg gcgccatcat
     8941 caccgtcggc agcctcgacg aggccctgcc cctgatcgag cggctggcgc ccgagcatct
     9001 cgaactgcac ctcagcgatg ccgaggcggt ggcaggccgg atccgccatg caggggcgat
     9061 tttcatcgga gcccatacgc cggaagccat cggcgactat gtcggcggct ccaaccatgt
     9121 cctgccaacc gcccgttcgg cgcggtttgc ctcagggctg ggcgtcatgg atttcgtcaa
     9181 gcgcacctcg ctgctcgcct gttcgccgga ggcgctcaga cagcttgggc cagcggcgat
     9241 ggtgcttggc gaatcggagg ggttgcaggc tcacgcgcgg tctgttgcta tccggctcga
     9301 cgatatcgac tgacggacag ccaatgagcg atgatgatcc ccgcaagaga ctttactccg
     9361 ttgtgctcga cgagacgtca atcgagcgcg gcacgccgga tcaggaacat gagcggcagg
     9421 tcgccatcta tgatctgatc gaggaaaact atttcgcgct cgctcatgat gacggcggcc
     9481 cgtatgcgct gcatctgggg ctggtgtcca accgcctggt gttcgatatc cggcgcgagc
     9541 agggagacac cgtggttgtg catgtgctgt cgctgacgcc gctgaagaaa atcgtgaagg
     9601 attatttcct catctgcgac agctattacg aggccatccg caatgccacg gcggaccgga
     9661 tcgaagccat cgacatggcc cgccgcgggg tgcacaatga ggggtctgcc atcgtgcgcg
     9721 agcggctgga gggcaagatc gaacttgatc accagacagc ccgccgcctg ttcacactga
     9781 tttccgccct gagctggacg gggcgggaag cgtgagcggg cctgccgcac cgatccactc
     9841 gatcctgttt ctgtgcacgc tgaattccgt tcgctctccg atggccgagg ggctggcccg
     9901 ccgcaagctc gggcaatacg tgcatgtcga aagcgccggc ctgatccgct ctgaacgcaa
     9961 tcccttcgca cttgccgtgc tgcgtgaaat cggcatcgat ttcagcgagg atgtgccgca
    10021 tgcgatgaat gcgctggcga tggaggggtt cgatcttgtc gttacccttt cggaccaggc
    10081 ccaggcagcg gtgatcgacc ggctgcgatt tgccagtata cggcacatcc actggtcgat
    10141 tcatgatccg accgaggctg gcggttcacg cgaggcgcgc cttcaggctt ttcggtccgt
    10201 gcgcgacgac ctgcgccaac ggatccgccg cgagctgctg ccgttgctgg aaaagtcaga
    10261 ctgatacctt tggctctcaa gattgacctt tcggcccgtt tcggagtgcc cgaccagaaa
    10321 aaaggccggc aggacgcaac ctgccggcct ttctgatgca taccgcgttt gagggatggt
    10381 acagccggct gggctcgaac cagcgacctt cggagccaca atccgacgct ctagccaact
    10441 gagctacggc tgcatgccct tcaaagcgga aagcgcgcgg accctatggg cattcgatcc
    10501 ggaattcaag ggccaagcct agcgtcggcg gcgaatttcg cagctgaatg aaacagggcg
    10561 ggccgaagcc cgccccgatc atgcgctctg tgcgggaggt cccgctatta cggcttgaag
    10621 ctgttggaca gcgccgactt caccggctcg gcggcatcgg cggcgagctt ctggccaagg
    10681 gccgcaagct cctgcacctg ggcctgaccg acctcaagct gcttcttgac gaactcggac
    10741 tgcagcgcga acacgtcctg cagggtcttg gcgccgaaca gcgcctgaac gtgatcgaac
    10801 gaaacgttgg cgttcttgcg agccagttcg atcgccttca gattgaaggc cacgacgccg
    10861 gacttggcag cgttgacgct ctcttcgagc ttgccagacg ccttctcggc cgcatccttg
    10921 acggcttcaa actggctgcg agcctgctca aggctcttct cagcggtctc gcgcatcttc
    10981 tcctgcatct ggctgatcgg agcaagcgcg gcctgaaccg cagtttcgac ggactcaacg
    11041 ggggaggtct tcttggcagc ggccatgaca cgaatccttg ggctcggggg acactcccgg
    11101 aggacccgcc caggaccttg ggagcgcagg ccactggcct gcctgattgg ctccctatag
    11161 caggtctatt gtgcattgca acataaatgc tgcgctcgca atgctgcaat gcacaaaatg
    11221 cgtaactggt gcgccagacg gcctatttgg ccggcttctt cggcgccttc ggcgtcacgg
    11281 cggcggttgc ggcgctggtg gcggcattgg ttgcctttgc caccgtggcc ttcaccgtct
    11341 cgaccgtcga ggccatgtgg ctggcggcat cttccatctg caatttcacg cgattgccgg
    11401 cttcctgcat ctgtgactgc agggcatgcg cctggctctg catgtattcg gtctgaatgc
    11461 gcagtgcctc ctcgatggtc ttggcctgaa cgagtttctg ggccatgtcg aaggcggcat
    11521 tcatgttgcg ttcggcaaat tcgaccgtca tgcgcgaggc gtcggcgaag ttcttgggaa
    11581 ccggaatcgg tccggcatgg acctgatcag acgctttctg ggccgcaccg ataaaggtct
    11641 cgaacgcctt gcgagcctgc tcgacgctgc ggctggcaag atcccgcatt tcgttcggca
    11701 cttcgaatgg attcttgagc atcgcttcct ccctgttcat gcagatttct atcatgccat
    11761 tggcggcggc gtccaccaat gggcattttg gcggacagca acaataccaa ccggcgcccc
    11821 gttaaggttg ataaacgatc gctgcctctc caactgtgcc attcatggac ggtttggccc
    11881 attcgcggta agaactggca aggatttatt aaggtttgtt cccagccggg ggattccgcc
    11941 cggcgggtgc tggtggagca aggcaaatgg ccgttctggc tgatcatggc attcttgccg
    12001 ctctggtggg agcggcacgg ggtgctgcgc tcgagcgcga tgagcgcgcg acggtcgttt
    12061 tcgacgagac cggagcgccg cgatgggcgt cgccatccgc acgcagcctg ttcggttccg
    12121 gcatggacgg ggctgtcgcg ttcagcgcgg cggctccggg cgcgcagcgg ctcaaggcgt
    12181 tcatcagggc ggccgacagc gaccggcttg ccgagcccct gcggttcttc atcggcgggc
    12241 ggcttgagcc cctgccggtt caggcgctgc gcctgaccct ggcggatgga acgcccggtc
    12301 tggtggcgct gttcggcgaa ggcccggccc tgaacctgcc tgatcccgag ccaaagaagg
    12361 cttccgagcc ggcgagcgcc gtgcagagcc ccctccccca acctgccgtg gagcaggacg
    12421 gcgcgcgcaa gacgcagcgg ttcgtgttcc tgaccgatgc cgaaggcgcg atttccagcg
    12481 tgtcgcctgt tttcacgcag gcattgggca tccatgcgcg tgatctggtc ggccgcaact
    12541 tcgtcgatgt gatcgccgag atcgacccgg tgcgcgccgg ggatgtggcg cgagccatgc
    12601 tcgccgcaac cacatggaac gggatctgcg tcgactggcc aatggcagac gggacgcgga
    12661 ccctgcctgt cgagttttca gcaatgccgg cgtttgacgc aagccgcgcc ctgaccggat
    12721 tcaacggctt cggcctgatc cggttcgagc gccctgccaa ctcggccaca gccagtccgg
    12781 ccgtgcccgc gcctgagccg gcactgccgg tgagcgagac cgcaccggcc acgacggaca
    12841 acgatttcga ggccgcaatc cggcggctgg aagcaattct gggtgacgtg ccgcaccacc
    12901 agcaggacgc gcgccccacc ccagacgcat cctccggcac agcggccggc aagcatctcg
    12961 cgccgaggcc tgaggggagg cccctgccct cgccggcccc gccggcggat gatgcctcgg
    13021 tggtgccgct gccctccctc aagacacatg tgggcgccac cctgggcgaa ccgaaagtag
    13081 tgccactgaa gccgaatctg ccgccggcca gcggcgcgca gaatgccggg ctcagccatt
    13141 cggagcgcaa cgccttccgc gagattgcca aggctctggg ggcgcggatc gaaacgacgc
    13201 cggttggcgg acacggcaag ggcaaggctg ccccgctgac gccgcaaacc cagaccgagg
    13261 tgccgccgaa ccgggttgcc gaggcacagg cggagctttc gaaggcgctc ggcgtgccgc
    13321 cggctatggc agcgggcgca caacagccat cggcaggctt taccgatctg ctgcttgagc
    13381 gtatgccgct gggcgttctg gttttccggg gcgacaagcc actctacctc aaccggacgc
    13441 tgctcgacct gaccggctac gacacactgg ccgaattcga tgcgctcaag ggactggaga
    13501 agctgttccg tggtcgcgcc gcaccggggc tggtgacggg cgaattcgat accgtgacga
    13561 tcatgggccg cgacggggcg acgctgccgg tcgatgcgca tctccaatcc atcgactggc
    13621 agggcgagca tgcgacgctg gtgaccatac gccgcgcaca ggatgccgaa gacggcccgc
    13681 gcgccaaggc gctcgatctc gaactgaaga agcggcaggc cgaactcggt gaagcgcagg
    13741 ccatgctgga tactgcaacc gatggcgttg tctcgctgga cgacggtgga cgcattctgg
    13801 cgctcaacaa gtccgccgag gcactgttcg gctacgatca gaacgaattg accggcgagc
    13861 ggctgacgct gctgctggcg cccgaaagcc acgctgctgc gctcgattat ctcgatggcc
    13921 tcaagtccgg cggggttgct tcggtgctca atgacggtcg cgaagtcatc gggcgcgagc
    13981 gcaagggcgg gcgcattcca ctgttcatga ccatcggccg gatctcggac accggcaagt
    14041 tcgccgccgt ggtgcgggac atgacggcct ggaagcgggc ggaagcggaa ctgaccgagg
    14101 ccaagcgcgc tgccgaacgt tcgagcgccc tgaaatcgga ctttctcgcc aaggtcagcc
    14161 atgaaatccg tacgccgatg aatgccatca tcggctttgc cgaggtgatg cgcgacgagc
    14221 ggctggggcc gatcggcacc gagcgctaca aggactatct caacgacatc catacctcgg
    14281 gcacgcatgt catcagcctg gtcaacgatc tgctcgacct gtcgaagatc gaagcggggc
    14341 ggatggaact caccttcgcc tcggtcgacc tcaacgcaat cgtcacctcc tgcctcggaa
    14401 tcatgcagcc gcaggccaac cgcgaccggg tgatcatgcg ctcgcagctt tcggccaaac
    14461 tgccaccggt ggtggcggac gaacggtcac tgcgccagat cgtattgaac ctgctgtcca
    14521 acgcgtccaa attcaccgaa ccgggcggac aggtgatcgt ctccaccgcc ctcaccgatc
    14581 aaggcgaggc ggtcatccgc gtccgcgaca ccggcgtcgg catgaccgag caggaaatcc
    14641 aggcggcgct ggaaccgttc cggcaattgt cgaacgcgcg cgggcgcggc ggcaccggac
    14701 ttggcctgcc gctgaccaag gcgctggtgg aagccaaccg cgccagtttc gtcatcaaca
    14761 gcaaggtgcg cgagggcacg ctggtggaga tcaccttccc gccgacgcgg gtgctggcgg
    14821 aataaggcag ggttgacgag tggcccgcac gcggtgcggg cgtccctcgc atcggctcgg
    14881 cgtcgcttcg ctcctatgag tggcccgcac acggtgcggg catacctcgc gtcagctcgg
    14941 cgtcgcttcg ctcctgtgca ctcctaccac cacagccagc cgcggcgcgg cgcatcgctc
    15001 accggcggat cgatcatctg gccggttgcc agcgagccag cggaaggcac cgaggcggtg
    15061 aagtcagcgt tctgctgctg cgcctcgcgc tgggcctggc gttgcgttcg cctggcgacc
    15121 agcgacgcca tcgcctgcgg ggtatcgagg ctgtagacgt catcacggaa atgggtcttg
    15181 ccgtcgtcgg tgacatagcc agtcagatag acccaagcga ccggcaccgg gcgcgcaagc
    15241 cgaatgtcct tgcgctcgcc ctcgctgatc gccgcctcga tcgtgctctt gtcccaattg
    15301 ccggcggtgc cttcgagcag ccaggcggcg agccccttca catcctgcac gcgcacgcag
    15361 ccatggctga agaaccggtc ctgcgcgttg aagaagcgct tggatggcgt gtcatgcata
    15421 tagaccgcat cgcggttggg catgtcgatg cggatctggc caagcgaatt ggtggagccg
    15481 gaatcctgcc tgaacgtgta gttcacggcc cgatccgtcg accagtcgat gctggacgca
    15541 ggcaccgtgt tgccggaacg gtcgaggatg cggatgttgg agcgggcaag gaagttgttg
    15601 gccggattgg accgcatctt cgggatgaac tcgttcttga tgatcgaggt cggaacggtc
    15661 caggtcgggt tgatattgac cgtgccaatg cgggcatcga cctgcggcga agcgttttcc
    15721 ggcttgccga caaccgcgac atagcggcgg cgcacgctgc cattctcgac agcctccacc
    15781 gaggccgaag ggatgttgac caccacataa cggccggaca ggttgaggtt gcggtcaacc
    15841 aggcgctggg cgctgttctc cagctgacgg gcgcgggtcg cagccgagac attgagcgcc
    15901 tccagtgtcg agcccgcaac cacgcccgag cgggccaggc cgtggcgcga ctggaactgc
    15961 ttgagcgcat cctggagcgc cttgtcaaat accggctggc tggtggcgcc ggcatcgaga
    16021 tcgccctcgg cggcaagccg ctggcgcagg gccaccaccg ccggcccctt cgagccaagc
    16081 gcaaggcgct gtttcggcag gctcggccag ccgcctgtct cggcaatctg gcgatagcgc
    16141 tgtgcggcct cctgaataag gcggatgctg tcggccacca gcgccggcag cggatcatcg
    16201 gtgacaatgc cgcgctcctt cggaggcggc cgcttgacga tcttcttctt cggctccgcc
    16261 ggggccgcgg caggggcgtt ggggtcggcc
//
LOCUS       DAOMXG010000038        15955 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_2050_length_15955_cov_9.719560, whole genome shotgun sequence.
ACCESSION   DAOMXG010000038 DAOMXG010000000
VERSION     DAOMXG010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 15955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15955
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2050_length_15955_cov_9.719560"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1274
                     /locus_tag="PLQ11_04260"
     CDS             <1..1274
                     /locus_tag="PLQ11_04260"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017486343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HRK24147.1"
                     /translation="RLSDARFFWETDRKVRLEERLRKLDTVTFHAKLGSQGERVTRIV
                     SLAKEIAPAVGADAGLAERAARLAKADLSTEMVGEFPELQGLMGRYYATLQGEDVKVA
                     AAIDDHYRPLGPSDRVPAEPVSIAVALADKLDTLIGFWSIDEKPTGSKDPYALRRAAL
                     GVIRIILDNGLRLPLGQIVSLRLVTNAMRAERGRQSDKAASTLARLEGLGSVRAVQSE
                     IATLVGQRKEDQSEFVASLEARVKAANATSVDLMAFFADRLKVQLREQGARHDLVDAV
                     LALPGQDDLLMAVRRIEALGSLLDSEDGTNLLAGYRRAANILKAEEKKDGASAFVGAV
                     DAASLVEAEETALAQSLNEVEQIAATAAAGEDFAGAMRALSRLRGPVDAFFDKVLVNA
                     ADPALRLNRLRLLTGLRRVMHTVADFSRIGG"
     gene            1462..4137
                     /gene="ppdK"
                     /locus_tag="PLQ11_04265"
     CDS             1462..4137
                     /gene="ppdK"
                     /locus_tag="PLQ11_04265"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017486150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HRK24148.1"
                     /translation="MTKWVYTFGDGKAEGQSAMKALLGGKGANLAEMCNLGLPVPPGF
                     TISTEVCTYFYDHDRTYPATLEAEVNAALDHVGRLTGRSFGDPRNPLLVSVRSGARAS
                     MPGMMDTVLNLGLNDVTVGALAASANDERFAWDSYRRFITMYSDVVLGVDHGHFEDIL
                     DQFKDLKGYALDTDVTAEDWKSLVVEYKACVERELGKSFPQDPREQLWGAIGAVFGSW
                     MNPRANKYRELHHIPASWGTAVNVQAMVFGNMGETSATGVAFTRNPSTGARELYGEFL
                     INAQGEDVVAGIRTPQDITEAARIAAGSTKASMETAMPAAYKELVRHYGLLERHYRDM
                     QDMEFTVEKGKLWMLQTRNGKRTAKAALRIAVDLAAEGLISRQEAVRRIDPSSLDQLL
                     HPTIDPKAHRKIIGTGLPASPGAATGEIVFTSEEAEQAVKDKRKVILVRIETSPEDIH
                     GMHAAEGILTTRGGMTSHAAVVARGMGKPCVSGAGTLRVDYQNGTLTAGPHKLKKGDF
                     ITIDGSTGQVLLGRIRMLQPELSGEFATLMGWADEVRRLKVRANAETPADARQAREFG
                     AEGIGLCRTEHMFFDENRIIAMREMILSDDEKGRRAALAKLLPMQRNDFEQLFEIMHG
                     LPVTIRLLDPPLHEFLPHTAEEIAEVAEAMGASPEKLRQRAAELSESNPMLGFRGCRL
                     AVAYPEIAEMQARAIFEAAVLAAKHNREPVVPEIMVPLVMNRAELDHVKSRIDAMARA
                     VMKEAGTRITYQVGTMIELPRAALRAAEIAQSAEFFSFGTNDLTQTTLGISRDDAANF
                     LEPYKRRGILAEDPFMTLDQEGVGELVQIAAERGRNTRPDLKLGICGEHGGDPASIGF
                     CEKTGLDYVSCSPFRVPIARLAAAQAALGSVTGSD"
     gene            4247..5284
                     /locus_tag="PLQ11_04270"
     CDS             4247..5284
                     /locus_tag="PLQ11_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HRK24149.1"
                     /translation="MTRRYVSRKRQARWGVPRWVLQSLAPWSVAACLLVSFTASAGPH
                     EDRSEAVFVRARKLVDLSLAPAAPSEAVRRVASLLFVDDAERLPPSPLRNFQEFDADA
                     LAALGRFVNDHSDDATTAAAEAAMTPGATQGHMNPAPPSQAADFALDGSTPAVARAVS
                     LGSTTPAPSEPETLVVAPKAGAPVLATAPVPLPPVRPVHTGYSDLIDPEDMDREQRCL
                     AEAVYFEARSEPPAGQAAVAQVVLNRVKSGLYPDSVCGVVYQNRHRYLGCQFTFACEG
                     KSLRVTEPGPWKLAKKVARDVTFGQTYLPDVGSATHYHANYVRPSWSRIFKKTDKIGK
                     HIFYKMRHNQT"
     gene            5503..6072
                     /locus_tag="PLQ11_04275"
     CDS             5503..6072
                     /locus_tag="PLQ11_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24150.1"
                     /translation="MRHHSIFALGGLVAALMATPASAGCYTAACYQKVVTPPVYGTVA
                     ETVQVRPASVARHVSPAQYGTVAEAVVVRPAQTVARHVPPVVRQVPETVQVSSGGRVW
                     QVSRDAYGREVGCWVDVPPKYATRLRTEVVQSGGIVHETIPAVTAVRHRTVMTRPAQV
                     HEHVIPAQYATRHHTVQVAPATASWQRLY"
     gene            complement(6136..7002)
                     /gene="nadC"
                     /locus_tag="PLQ11_04280"
     CDS             complement(6136..7002)
                     /gene="nadC"
                     /locus_tag="PLQ11_04280"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016919237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HRK24151.1"
                     /translation="MTARPDLAPLPAPLVRAAIARALEEDLGLAGDITAQACLPADAR
                     ARLTMISRRAGVVSGTQLAVEAFRQMDPAAAVTVLKPDGSRLAPRDAILIVEGNARAA
                     LSAERSALNYMGRMSGIATLTAAYVDQVKHTATRILDTRKTTPGLRALEKYAVLCGGG
                     VNHRFGLFDCILIKDNHIGVAGGVKPALRRAKLHAGSTVKIEIEVDRLSQLDEVLEEG
                     ADIVLLDNMDLPTLREAVRRARGKIITEASGGVTLETVKAIAETGVDFISSGALTHSA
                     PVLDIGLDIAIG"
     gene            complement(7009..8535)
                     /locus_tag="PLQ11_04285"
     CDS             complement(7009..8535)
                     /locus_tag="PLQ11_04285"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HRK24152.1"
                     /translation="MSSTLIIGGGLAGLFTALKLAPAPCLVIAPKPLGQGASSAWAQG
                     GVSAAIQPGDSIDSHVADTVKAGAGLVNEAVTRGMAGEASDRIHDLVEYGVPFDRDLA
                     GHLVASREAAHSHARVVRVKGDMAGAKIMEALIAAVRHAPSIHVLDGAEALNLVIENG
                     RIAGANILLDGKVMRARAGQVVLATGGIGHLYAVTTNPPQARGQGLGMAALAGAVVRD
                     PEFVQFHPTALNVGRDPSPLLTEALRGEGAILVNGAGERFMTALHPDAELAPRDVVAR
                     GVFAEIKAARGAFLDARFAVGSAFAEKFPTVYAAAMSAGIDPVHDLLPVCPAQHYHMG
                     GVLTDARGQTSIPGLYAVGEVASSGVHGANRLASNSLLEAVVFAARIAEQLRPVTNRA
                     VTLLPDASPVLPHEDHGGIEPLRAFMMLHVGVTRGEKELAETLRLIRRMQLPERLEPS
                     IRNALVAAELIVTGALLRHESRGGHARYDYPDTDAVARHTIFTLAEARRSADTALARL
                     "
     gene            complement(8597..9664)
                     /gene="nadA"
                     /locus_tag="PLQ11_04290"
     CDS             complement(8597..9664)
                     /gene="nadA"
                     /locus_tag="PLQ11_04290"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HRK24153.1"
                     /translation="MANAPHTGSIEQRTSARGYEPLPMPDLTYTPEIARATDHLYEKV
                     SHIIPPVEWPFHAPYVAAINRLKKQRDAVILAHNYQTPEIYNCVADVVGDSLQLARLA
                     GKVPAKVIIQGGVHFMAETSKLLNPEKTVLIPDLKAGCSLASSITGADVRLLRQRYPG
                     VPVVAYVNTSADVKAEVDICCTSSNAVHVVESLGVDQVLFVPDQYLAKYVASKTKVKI
                     ISWHGACEVHERFTGEELKRYREGDPTLKIIAHPECPPDVIAEADFTGSTAGMIDYAK
                     AQRGGRVLLVTECSMADNVAASARDVEFVRPCNLCPHMKRITLPKILDSLIYMQHEVV
                     IEPELAARARRSVERMVNLNN"
     gene            9940..11181
                     /locus_tag="PLQ11_04295"
     CDS             9940..11181
                     /locus_tag="PLQ11_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRK24154.1"
                     /translation="MTQPPISSADRKDAASPLAIILAGCILSMLTFGPRGAMGFFQLP
                     VISDHGWGRDTFGFALAVQNLLWGLGQPFAGAVADRFGAVRVLVAGLVTYAASLVLMS
                     VSSTPLAFNITAGVMFGFALSGCSFNLVLAAFGKLLPPEWRAMAFGFGTASGSFGQFL
                     FSPLSVGLIDAFGWRGALYVFAGLLLVAMPLAFLLRTDGSTAATSASGEPRTGLAETL
                     ARAFSHRSYVLLVLGFFTCGFQLAFITVHLPAYLRDAGLSPAIGGWVVALIGLFNVLG
                     SLSSGWLCNRMPKRYLLAIIYALRSLAILALIALPVTPATALFFGASMGLLWLSTVPP
                     TSSLVALMFGTRDMAMLYGFVFFSHQVGGFLGVWLGGIFYEAQGNYDLIWYASIALGI
                     ASAIVNLPIEEKAAPVRVATA"
     gene            11261..12253
                     /locus_tag="PLQ11_04300"
     CDS             11261..12253
                     /locus_tag="PLQ11_04300"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003607198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HRK24155.1"
                     /translation="MGDFRALVVNKDANGYRCEDRRITEADLMPGNVTVRVTHSTVNY
                     KDGLAITGKSPVVRTFPLVPGIDFCGIVEHSQDPRYQAGDAVILTGWGVGEVHSGGYA
                     EKAQVNGDWLVPLPKGLTAARAMAIGTAGFTAVQSVLALEKFGLKPAAGPVIVTGAAG
                     GVGSVAVAVLARLGWHVIASTGRMAEADYLKGLGASEVIDRAELSGPAKPLARERWAA
                     GVDSVGSTTLANVLSMTRSNGAIAACGLAQGMDLPTSVAPFILRGVALLGINSVPAPR
                     DLRIEVWNRVVRDMDMDKLDAMTRTIGFGEIMPTARAILDGKVRGRTVVDIVRD"
     gene            complement(12275..13180)
                     /locus_tag="PLQ11_04305"
     CDS             complement(12275..13180)
                     /locus_tag="PLQ11_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRK24156.1"
                     /translation="MQTIEWDDFRLVKVIAEHRSLGPAAEALKLNHSTVFRKLNALEE
                     QLGTRLFERSRNGYSLTASGEEMLVIAGQMADAVFDFERRLAGMDARPTGTLRVTTTE
                     SMLENLLAPIFTSFRLLYPGIHLEVLVDARPLNLSRRDADVAIRAAMEPTDTLVGRRI
                     ASMAWAAYAHKDKMPPGPVNLFNDDLSWIGFGDPVSLSVAGRWFANHIPHHRMAMTVN
                     AVSSMARLAQAGAGVAMLPCFVGDRQDSLRRIAEPVKDATSTLWLLTHSDLKNAVRIR
                     AFLDFVGAELIRQRRLIEGLEPQPA"
     gene            complement(13252..14235)
                     /locus_tag="PLQ11_04310"
     CDS             complement(13252..14235)
                     /locus_tag="PLQ11_04310"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRK24157.1"
                     /translation="MTMRLPPDPAGIAEAARLIRAGGLVALPTETVYGLGADATNPAA
                     VARIYAAKGRPAFNPLIAHLATLEAAQAEGTLDAAALRLARQFWPGPLTLVVPRIEGG
                     KVCDLACAGLASVGLRIPSHPVALDLLKAAGRPVAAPSANRSGHVSPTLPEHVLADLD
                     GVIDAVVMAGPCPVGVESTIVGCLGNEAVLLRPGGITRADLSSSLGEPVRDPVQTDDN
                     QTPQSPGRLTAHYAPETPLRLNATSVSPGEALLAFGPALPSGADKAHIVQNLSRSANL
                     TEAATHLYTFLRNLDKLRASAIAVAPIPESGLGEAINDRLRRAAAAKSVCN"
     gene            14378..>15955
                     /locus_tag="PLQ11_04315"
     CDS             14378..>15955
                     /locus_tag="PLQ11_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRK24158.1"
                     /translation="MSYRAPVDDILSAMIHAGGLGRGITSGIYADLGDGVAEAILEEA
                     GKFAADRLAPLNKVGDRHGTPLKDGRVTMPPGWLEAYRDWAAAGWCSIVASPDFGGQG
                     LPTLLNSACTEIWNSANMGFSLNPLLTMGAVEALTAHGSDTLKETYLTRMIAGEWTGT
                     MNLTEPGAGSDLAQIRTRAERAGDGSYRITGSKIFITYGEHDLTENILHLVLARLPDA
                     PAGTRGISLFLVPKFLVNADGSLGARNDVRCHSIEHKMGIHASPTCTMVFGDNGGAVG
                     YLVGEENRGLACMFTMMNNARLNVGLQGVAIAERAYQQALAYAQERKQGKAIGSTAAG
                     PEAIIAHPDVKRMLLDMRTKTAAARALCYATAGALDRAERLTDPAAKKAAHEEASLLT
                     PIAKAFSTDIGIEVASTGVQIHGGMGFIEETGAAQHLRDARIAAIYEGTNGIQAIDLV
                     TRKLPLADGETVKDVIHACRTAADKAAAINDPGFARIGRILRDAVGSLERCTDFMMKA
                     LKAAPNDALAGATPYLRL"
BASE COUNT         2744 a         5055 c         5253 g         2903 t
ORIGIN      
        1 cccgcctgtc ggatgcgcgg ttcttctggg aaaccgacag gaaggtccgg ctggaagagc
       61 ggctcaggaa gcttgataca gtcactttcc acgccaaact cggctcgcag ggcgagcggg
      121 taacgcgcat cgtgtcgctg gccaaggaga tcgcgcctgc tgtcggcgcc gatgccgggc
      181 tggccgagcg ggcggcgcgg ctggcaaagg cggatctgtc cacggagatg gtcggcgaat
      241 tccccgaact gcagggactg atgggccgct attatgcgac cttgcagggc gaggatgtca
      301 aggttgccgc cgcgatcgac gatcattacc ggccgcttgg cccgtcggat cgcgttccgg
      361 ccgagccggt gagcatcgcc gtcgcccttg ccgacaagct cgacacgctg atcggcttct
      421 ggtcgatcga cgaaaaaccc accggctcca aggaccctta tgcgctgcgc cgcgctgcgc
      481 ttggcgtcat ccggatcatt ctcgacaatg gcctgcgtct cccgctgggc cagatcgttt
      541 cgctgaggtt ggtcaccaat gcgatgcgcg ccgagcgggg ccggcagagc gacaaggcgg
      601 cgtccacgct ggcgcggctc gaagggctgg gctcggtgcg cgccgtccag tccgagatcg
      661 ccacccttgt cggccagcgc aaggaggacc agagcgaatt cgtcgccagt cttgaggcgc
      721 gggtcaaggc tgccaatgcc acctccgtcg atctgatggc gtttttcgcc gaccggctga
      781 aagtgcagtt gcgcgagcag ggcgcccgtc atgatctggt cgatgcggtg ctggctctgc
      841 ctggacagga cgatcttctg atggccgttc gccgcatcga ggcgctgggc tcgctgcttg
      901 acagcgagga cggcaccaac ctgctcgccg gttatcgccg cgctgccaac atcctcaagg
      961 cggaggagaa gaaggacggg gccagtgcct tcgtcggcgc tgtggatgcc gcatcgctgg
     1021 tcgaggcgga ggaaactgcg ctggcgcagt cgctgaatga ggtggagcag atcgccgcca
     1081 cggccgctgc cggcgaggat ttcgccggcg ccatgcgggc gctgtcccgc ctgcgtggcc
     1141 cggtcgatgc attcttcgac aaggtgctgg tcaatgccgc tgatcccgcg ctccgcctca
     1201 accggctgcg cctgctcacc ggactcaggc gagtgatgca tactgtggct gatttttccc
     1261 gcatcggtgg gtgacgaccc ggcacgacag ccatgcgccg gttgcagagc tggcgcggcc
     1321 tttttcatac tggtgtagaa tcgccgccgg cattaacgat gaacacaatg gcggaggtgt
     1381 tttccgtggt tccgttcagg caactgaaag gcactttgga taggaccgat caggcaaaag
     1441 accgcagatg cgaggcgctc catgacgaaa tgggtctata cgttcgggga tggcaaggcc
     1501 gaaggccagt cggcgatgaa ggccctgctt ggcggcaagg gggccaatct ggcggaaatg
     1561 tgcaacctcg gcctcccggt gccgcccggc ttcaccattt ccaccgaggt ctgtacctat
     1621 ttttacgacc atgaccggac ctatcccgcc acgctggagg cggaggtcaa tgccgcgctc
     1681 gatcatgtcg gccggctgac cggtcgcagc tttggcgatc ccagaaatcc gctgctcgtt
     1741 tcggtgcgtt cgggggcccg cgcttccatg cccggcatga tggataccgt gctcaatctc
     1801 ggcctcaatg atgtgaccgt cggggcgctg gcagcgtccg ccaatgacga gcgcttcgcc
     1861 tgggacagtt accgccgctt catcaccatg tattccgacg tggttctcgg cgttgaccat
     1921 ggtcatttcg aggacattct cgaccagttc aaggacctca agggctatgc cctggatacc
     1981 gacgtcacgg ccgaggactg gaagagcctg gtcgtcgaat acaaggcctg cgtcgagcgg
     2041 gaactgggca agtcgtttcc gcaggacccg cgcgaacaat tgtggggcgc catcggcgct
     2101 gtcttcggct cgtggatgaa cccgcgcgcc aacaagtatc gcgagctgca ccatatcccg
     2161 gccagctggg gcacggcggt caatgtacag gcgatggtgt tcggcaacat gggcgaaacc
     2221 tcggccaccg gcgttgcctt cacccgcaat ccgtcgaccg gcgccaggga gctttacggt
     2281 gagttcctga tcaacgcgca gggcgaggat gtggtggccg gcattcgcac cccgcaggac
     2341 atcaccgaag cggcgcggat cgccgccggc tcgaccaagg cttcgatgga aacggccatg
     2401 ccggccgcct acaaggagct ggtgcggcat tatggactgc ttgagcggca ttaccgcgac
     2461 atgcaggaca tggaattcac ggtcgagaag ggcaagctgt ggatgctgca gacccgcaac
     2521 ggcaagcgca ccgccaaggc ggcgctgcgc attgcggttg atctcgccgc cgaaggtctg
     2581 atcagccggc aggaagctgt gcggcggatt gatccgtcat cgcttgatca gctgctgcac
     2641 ccgaccatcg accccaaggc ccatcgcaag atcatcggca ccggcctgcc ggcgtcaccg
     2701 ggcgcggcga ccggagaaat cgtctttacc tccgaagagg ccgaacaggc ggtcaaggac
     2761 aagcgcaagg tcattctggt gcgcatcgag acctcgccgg aggacattca cggcatgcat
     2821 gccgccgagg gcatccttac cacccgcggc gggatgacca gccatgccgc ggtcgtggcg
     2881 cgcggtatgg gcaagccctg cgtctccggc gccggcaccc tgcgcgtcga ctaccagaac
     2941 ggcacgttga ccgcgggtcc gcacaagctg aagaagggcg atttcatcac catcgacggg
     3001 tcgaccggac aggtgctgct cggacgcatc cgcatgttgc agccggaact gtcgggcgaa
     3061 ttcgccaccc tgatgggctg ggccgacgag gttcgccgcc tcaaggtccg cgccaatgcc
     3121 gaaacgccgg ccgacgcccg ccaggcgcgc gagttcggcg ccgaaggcat cggtctgtgc
     3181 cgcaccgagc acatgttctt cgatgagaac cgcatcattg ctatgcgcga gatgatcctc
     3241 tccgacgacg agaagggccg ccgcgccgcg ctcgccaagc tgctgccgat gcagcgcaac
     3301 gatttcgagc agctgttcga gatcatgcac ggattgccgg tgaccatccg cctgctcgat
     3361 ccgccgctgc atgaattcct gccgcacacg gccgaggaaa tcgccgaggt tgccgaggcg
     3421 atgggcgcca gccccgaaaa gcttcgccag cgtgcggcgg aactgtccga gtccaatccg
     3481 atgctcggct tccggggctg ccgtcttgcc gtggcctacc ccgagatcgc cgagatgcag
     3541 gcccgggcca ttttcgaggc ggcggtgctg gccgccaaac acaaccgcga gccggtggtg
     3601 cccgagatca tggtgccgct ggtgatgaac cgggccgaac tggaccatgt caagtcgcgg
     3661 attgatgcca tggcgcgtgc ggtgatgaag gaggccggca cccgaatcac ctaccaggtc
     3721 ggcacgatga tcgaattgcc gcgcgccgcg ctgcgcgccg ccgagatcgc ccagtcggcg
     3781 gagttcttct cgttcggcac caacgacctg acccagacga cgctgggcat cagccgtgac
     3841 gatgctgcca acttcctcga accctacaag cggcgcggca ttctggccga ggatccgttc
     3901 atgacgctgg atcaggaagg ggttggcgaa ctggtgcaga ttgccgccga gcgcggccgc
     3961 aacacgcggc cggacctcaa gcttggcatc tgcggcgagc atggcggcga tccggcctcg
     4021 atcggcttct gcgaaaagac cgggctcgac tatgtgtcct gctcgccgtt ccgcgtgccg
     4081 atcgcgcggc tggcggcggc gcaggcggcg ctcggcagcg tcaccggctc cgactgaccg
     4141 ggcgttaacc gcccggcaac catgctgatc gacaatcggc atgggattat tggcgttgcg
     4201 caaccgttga aaccggtgcg tagcgcggtc ggagcggatc ggcagcgtga ccagacggta
     4261 tgtcagtcgc aaacggcagg cgcggtgggg tgtgccccgc tgggtgctgc aatcgcttgc
     4321 gccctggtcg gtggcggcct gcctgctggt ttccttcacc gccagcgccg gcccgcacga
     4381 ggaccgctct gaggcggtgt ttgtccgcgc ccgcaagctt gtcgatctgt cgctggcacc
     4441 ggccgcgccg agcgaggcgg ttcgccgggt ggcatccctg ctgtttgtcg acgatgccga
     4501 gcgcctgccg ccgtcgccct tgcgaaattt tcaggaattt gatgccgatg cgctggccgc
     4561 gcttggccgc ttcgtcaatg accattcgga cgatgccaca acggcggccg ccgaagcagc
     4621 gatgacaccg ggcgcaaccc agggacacat gaacccggcc ccaccgtctc aggctgccga
     4681 tttcgcgctt gatgggtcaa ccccggctgt tgcccgggcc gtgtcactcg gttcgacaac
     4741 gccggccccg tcggagcctg aaacgctggt tgtcgcgccg aaggccggtg cgccggtgct
     4801 ggccacggcc cccgtgccgc tgccaccggt gcggccggtg cacaccggct acagtgatct
     4861 gatcgacccg gaggatatgg acagggagca gcgctgcctc gccgaggcgg tgtatttcga
     4921 ggcacggtcc gaaccgcccg ccggacaggc tgcggtggcg caggtggtgc tcaaccgggt
     4981 gaaaagcgga ctttatccgg acagcgtctg cggcgtcgtc taccagaatc gccaccgcta
     5041 tcttggctgc cagttcacct tcgcctgcga gggcaagtcg ctgcgcgtca ccgagccagg
     5101 gccttggaaa ctcgccaaga aggtcgcccg cgacgtcacc ttcggccaga cctacctgcc
     5161 cgatgtcggt agcgcgacgc attatcacgc caattatgtg cgcccatcct ggtcacgaat
     5221 attcaagaag acagacaaga tcgggaagca tatcttctac aagatgcgcc acaatcagac
     5281 atgatttggc tgtcacgtat gaatctgtag tatattttac aaaaaatttt acgtatattt
     5341 gttcagaaat gaaacaatcg gactcgggga acatgataac aatcttagtt aaatttacct
     5401 ttacatgatg atcgtcttgc agaaatgcag actttgaggc cctatggcga caacaccaca
     5461 cggatcaacg tgcaggtagg gtgtcaaaac ggggagtgcc ttatgcgtca tcattcgatt
     5521 tttgcccttg gtggcctggt tgcggccctg atggccacac cggcctcggc cggttgctac
     5581 acagccgcct gctatcagaa ggtcgtcacg ccgccggtct acggcacggt cgccgagacg
     5641 gttcaggtgc gcccggcgag cgtcgcccgg catgtcagtc ccgcccagta tggcacggtg
     5701 gccgaagcgg tcgtggttcg tccggcccag acggtcgccc gccatgtacc gccggtcgtc
     5761 cggcaggttc ctgaaacggt gcaggtttcc tcaggcggtc gcgtctggca ggtgagccgc
     5821 gatgcttatg gccgcgaggt cggctgctgg gttgatgttc cgccgaaata cgcgacccgt
     5881 ctgcgcaccg aggtggtgca gtccggcggc atcgtccacg aaaccatccc tgcggttacg
     5941 gcggtccgtc accgcacggt gatgacccgc ccggcacagg tgcatgagca cgtgatcccg
     6001 gcccagtatg cgacccgcca ccacacggtg caggtggctc cggcgaccgc ctcctggcag
     6061 cgcctctact gagtttgtga tccgacgatc cgaaagcgcc ggccgcaggg ccggcgtttt
     6121 tgctttggcg cccgttcagc cgatcgcaat atcgagcccg atgtcaagca ccggcgccga
     6181 atgggtcagc gcgccggacg aaatgaaatc gacgccggtt tcggcgatgg ctttcaccgt
     6241 ctccagcgtc accccgccag aggcctcggt gatgatcttg ccgcgtgcgc gcctgactgc
     6301 ctcgcgcaag gtcggcaggt ccatgttgtc gagcaggacg atatcggcgc cttcctccag
     6361 cacctcgtca agctgcgaca gccggtcgac ctcgatctcg atcttcaccg tcgagccggc
     6421 atggagcttg gcccggcgca gcgcgggctt gaccccgccg gcgacgccga tgtggttgtc
     6481 cttgatcagg atgcaatcga acaggccgaa gcggtggttg acgccgccgc cgcacagcac
     6541 ggcgtatttc tccagcgcgc gcaggccggg ggtggtcttg cgcgtgtcga gaatgcgcgt
     6601 ggccgtgtgc ttgacctgat cgacataggc agcggtgagc gtggcgatgc cgctcatccg
     6661 ccccatgtag ttgagcgcgg atcgctccgc cgacagggct gccctggcgt tgccttccac
     6721 gatcaggatc gcgtcacgcg gggcgaggcg ggaaccatcc ggcttcagaa cggtgacagc
     6781 ggcggcagga tccatctggc ggaaggcttc caccgcaagc tgggtgccgg aaacgacgcc
     6841 ggccctgcgg ctgatcatcg tcagccgcgc ccgcgcatca gccggcaggc aggcctgcgc
     6901 ggtgatgtcg ccggcaaggc caaggtcctc ctccagcgca cgggcaattg cggcgcggac
     6961 cagaggggcg gggagagggg caaggtcggg acgtgccgtc attgatgtct agagccgggc
     7021 tagggccgta tcggcgctgc ggcgcgcctc ggcgagggtg aatattgtgt ggcgggccac
     7081 agcgtcggtg tccggatagt cgtagcgggc atgcccgccc cggctttcat gccgcagcag
     7141 cgcgccggtc acgatcagtt cggcggcgac cagcgcattg cggatcgagg gctccagccg
     7201 ctcgggaagc tgcatgcggc ggatcagccg cagcgtctcg gccagttcct tttctccgcg
     7261 cgtgacgccg acatgcagca tcatgaaggc acgtagcggc tctatcccgc cgtgatcctc
     7321 atgcggaagg accgggctgg catccggcag cagcgtgacg gcacggttcg tgactgggcg
     7381 cagctgctct gcgatgcgcg ctgcaaagac cacggcctcc aacagcgaat tggaggccag
     7441 ccggttggcg ccatgcacgc cgctcgatgc gacctctccg acggcataaa ggcctgggat
     7501 cgaggtctgg ccgcgcgcat cggtgagaac accgcccatg tgataatgct gcgccgggca
     7561 gaccggcagc agatcatgca cggggtcgat gcctgcggac atggcggcgg catagacggt
     7621 gggaaacttt tcggcaaaag cactgccaac cgcaaaacgc gcatcgagaa acgcgccgcg
     7681 tgccgccttg atctcggcaa agaccccgcg cgccaccaca tcgcggggtg ccagttcggc
     7741 gtcaggatgc agcgcggtca tgaaccgctc gccggcaccg ttgaccagga tcgcgccctc
     7801 gccgcgcagc gcctcggtca gcagcggcga gggatcgcgc ccgacattga gtgcggtggg
     7861 gtgaaactgc acgaattcgg gatcgcgcac caccgcgccc gcgagcgccg ccatgccaag
     7921 cccctgtccg cgcgcctgcg gcggattggt tgtcaccgca tagagatgac cgataccacc
     7981 cgtcgccagc acgacctggc cggcgcgcgc gcgcatcacc ttgccgtcga gcaggatgtt
     8041 ggcgccggca atgcggccgt tctcgatgac cagattcagg gcctccgcac catccagcac
     8101 atggatcgac ggagcatgtc gcacggcagc gatcagtgct tccatgatct tggcgccggc
     8161 catgtcgccc ttgacgcgga cgacgcgcgc atggctgtgg gcggcctcgc ggctggcgac
     8221 aagatggccg gccagatcgc ggtcgaacgg cacgccgtat tcgacgagat cgtggatgcg
     8281 gtcgctggct tcccccgcca tgccgcgcgt caccgcctca ttcaccagcc cggcgccggc
     8341 cttgaccgta tcggcgacat ggctgtcgat gctgtcgccc ggctggatcg ccgccgaaac
     8401 cccgccctgc gcccaggccg aggaggcgcc ctgacccagc ggctttggcg cgatgaccag
     8461 acagggagcc ggcgccagct tcagcgccgt gaacaacccg gccaacccgc cgccgatgat
     8521 aagggtcgag gacatcgcgc tccctgcctt gtctcagagc ggcaagatgc cagtgcggca
     8581 ccccgatggc cgcatctcaa ttgttgagat tgaccatccg ctccaccgaa cgccgcgcgc
     8641 gagcggcaag ctccggctcg atcaccactt catgctgcat gtagatcagg ctgtcgagga
     8701 tcttcggcag cgtgatgcgc ttcatatgcg ggcagaggtt gcagggacgc acgaattcga
     8761 catcgcgcgc gctggccgcc acattgtccg ccatcgagca ttcggtgacc agcagcaccc
     8821 gcccgccgcg ctgggccttg gcgtagtcga tcataccggc ggtcgatccg gtgaaatcgg
     8881 cctcggcgat cacatccggc gggcattcgg ggtgggcgat gatcttgagg gtagggtcac
     8941 cctcgcgata gcgcttgagt tcctcgccgg tgaaccgctc gtgcacctcg caggcgccat
     9001 gccaggaaat gatctttacc ttggttttcg aggcgacata tttggccaga tactgatccg
     9061 gcacgaacag cacctgatcg acgccaaggc tttcgaccac atgcaccgca ttcgacgagg
     9121 tgcagcaaat atcgacctcc gccttcacat cggccgaggt gttgacatag gcaaccaccg
     9181 gaacacccgg ataacgctgg cgtagcagcc gcacatccgc cccggtgatc gacgaggcaa
     9241 gcgagcagcc agccttgaga tcggggatca gcacggtctt ttcgggattg aggagtttgg
     9301 aggtctcggc catgaaatgc acgccgccct ggatgatgac cttggcgggc accttgccgg
     9361 cgagccgcgc cagctgaagc gaatcaccca ccacatcggc aacgcaattg tagatttccg
     9421 gcgtctggta attgtgcgcg aggatcaccg catcgcgttg cttcttcagc cggttgatgg
     9481 ccgcgacata cggcgcatgg aacggccatt cgaccggcgg aatgatatgg gagaccttct
     9541 cgtaaagatg gtccgtcgca cgcgcgatct ccggcgtgta agtcaggtca ggcatcggca
     9601 gcggctcgta gccccgcgcg gacgtgcgct gttcaatcga tccggtgtgc ggggcattcg
     9661 ccatggtcat ctccttatac tcacaatgag tatataaggg ccaaaaaaga aaatcggcaa
     9721 agccgacttt atactcatat tgagcattat tataggacgg aaccaacgcc gtggctagta
     9781 aaatcttcgc ggctcgccgc agctggtgag ggatggggca gaccgcatgc aaggggcggg
     9841 ctggtggaca attttagatc actaaatatt gaggataagc cgttgcgttg tggttttgcc
     9901 tgtccctgtg tcgcggtatg gtgggcctga gaggctatca tgacacaacc acccatttca
     9961 tcggcggacc gcaaggacgc agcgtcgcct ttggcaatca ttctggccgg atgcatcctg
    10021 tcgatgctga ccttcggtcc acgcggcgcg atggggttct ttcagttgcc ggtgatcagt
    10081 gatcatggct gggggcgcga cacattcggc ttcgcgcttg cggtgcagaa cctgctctgg
    10141 ggccttggcc agccctttgc aggtgcggtt gcggaccgat ttggcgcggt gcgtgtgctg
    10201 gttgccggcc ttgtcaccta cgcagccagc cttgtgctga tgagtgtctc ctcgacgcct
    10261 ctggccttca acatcacggc gggggtgatg tttggttttg ccctgtcggg ctgttcgttc
    10321 aatctggttc tggcggcgtt cggcaagctg ttgccgcccg aatggcgggc gatggccttt
    10381 ggtttcggca cggcttcggg ctcgttcggc cagttcctgt tttcgcccct gtcggtggga
    10441 ttgatcgatg cctttggctg gcgtggcgcg ctttatgtgt ttgccgggtt gttgcttgtg
    10501 gcgatgccgc tggcgttcct gctgcggacg gacggcagta cggccgccac ctctgcgtcc
    10561 ggggagccgc gcaccggcct ggcggaaacc ctggcgcggg ccttttcgca tcgctcctat
    10621 gtcctgctgg ttctgggctt tttcacctgc ggcttccagc ttgccttcat cacggtgcat
    10681 ctgccggcct atctcaggga tgccgggctc agcccggcga ttggcggctg ggtcgtggca
    10741 ttgatcggcc tgttcaacgt tcttggatcg ttgtcgtccg gctggctgtg caaccgcatg
    10801 cccaagcgct atctgctggc gatcatctat gccttgcgct cgctggcgat ccttgcactg
    10861 atcgccttgc cggtgacgcc ggcgacagcc ttgttctttg gcgcctcgat gggactgctg
    10921 tggctgtcca ccgttccgcc gacctcctcg ctggtcgccc tgatgttcgg cacgcgggac
    10981 atggcgatgc tgtatggctt tgtgttcttc tcccatcagg tgggcgggtt cctcggagtc
    11041 tggctgggcg gcattttcta cgaggcgcag ggcaattacg acctcatctg gtatgcctcg
    11101 atcgcgctgg gcatcgcatc ggcgatcgtc aacctgccga tcgaggaaaa ggccgcaccc
    11161 gttcgggtgg caaccgcctg aagcggcgtt cgacctttgg ggacatgacc ggcgtggcca
    11221 gcccgtatag ggtgcaacaa caactcagcg tgagggcatc atgggcgatt tccgggcatt
    11281 ggtcgtcaac aaggacgcaa acggctaccg ctgcgaggac aggcgcatca ccgaggcaga
    11341 tctgatgccc ggcaatgtca ccgtgcgcgt cacccattca accgtgaatt acaaggatgg
    11401 cctggcgatc accggcaagt cgccagtcgt gcgcacattc ccgctggtgc caggcatcga
    11461 tttttgcggc attgtcgagc actcccagga tccccgctat caggccggcg atgcggtcat
    11521 tctcaccggc tggggggttg gtgaggtgca ttcgggcggc tatgcggaaa aggcccaggt
    11581 caacggcgac tggctggttc cgctgcccaa ggggcttacg gcggcccgtg cgatggccat
    11641 cggcaccgcc ggcttcacgg cggtccagtc cgtgctggcg ctggaaaaat tcggcctcaa
    11701 accggcggct ggcccggtga tcgtcaccgg cgcggcgggc ggtgtcggtt cggtggcggt
    11761 ggcggtgctg gcccggcttg gctggcacgt catcgcctcg accggacgga tggcggaggc
    11821 tgactacctc aaggggctcg gcgccagcga ggtcatcgac cgggcggaat tgtcggggcc
    11881 ggccaagccg ctggcgcgcg agcgctgggc ggcgggggtc gattcggtcg gatcgaccac
    11941 tttggccaat gtcctgtcga tgacccgctc gaatggggcg atcgccgcct gcggcctcgc
    12001 ccaggggatg gatctgccaa cctctgtcgc gccgttcatc ctgcgcggtg tcgcgctgct
    12061 cggcatcaat tccgtgccgg cgccgcgcga tctgcggatc gaggtgtgga accgggtcgt
    12121 gcgcgacatg gatatggaca agctcgacgc gatgacccgc acgatcggct ttggcgagat
    12181 catgccgacg gcgcgcgcca ttctcgacgg caaggtgcgc gggcgaacgg tggtggacat
    12241 cgtccgcgat tgaggcgact ggcggccagt ccgctcatgc cggctgcggt tcaagccctt
    12301 cgatcagtcg gcgctggcgg ataagctcgg caccgacgaa atcgaggaaa gcacggatcc
    12361 gcaccgcgtt cttgaggtcg gaatgggtca gcagccataa cgtcgatgtc gcatccttga
    12421 ctggctcggc gatgcgccgc agggagtcct gcctgtcgcc gacgaaacag ggcagcatgg
    12481 caacgccggc gcccgcctgt gccaggcgcg ccatcgacga tacggcattg accgtcatgg
    12541 ccatccgatg gtgcggaata tggttggcga accatcgccc ggccaccgac agcgacaccg
    12601 gatcgccaaa tccgatccag ctcagatcat cattgaagag gttgaccggc cccggcggca
    12661 tcttgtcctt gtgcgcatag gcggcccagg ccatgctggc gatgcggcgg ccgaccaggg
    12721 tgtcggtcgg ctccattgca gcacggatgg cgacgtctgc atcgcggcgg gacaggttga
    12781 gcggccgggc gtcgaccagc acctcaagat gaatgccggg gtagagcagg cggaacgatg
    12841 tgaagatcgg cgccagcagg ttctccagca tggattcggt cgtcgttacc cggagggtgc
    12901 cggttggtcg cgcatccatt ccggcaagcc gtcgttcaaa atcgaaaacg gcgtccgcca
    12961 tctggccggc aatgaccagc atctcctcgc ccgacgcggt cagcgaatag ccgttgcgcg
    13021 atcgttcgaa caggcgcgtg cccagctgtt cttccagcgc gttgagcttg cggaagacgg
    13081 tggaatggtt gagcttcagt gcctcggctg ccggaccgag ggagcgatgc tcagcgatca
    13141 ctttgacgag acggaaatcg tcccactcga tggtttgcat gagaaccaat atgccacaac
    13201 ttcagagtgt ccgcacgcat tcatgaaatt aggcgtaaat gcaagctttg cttaattgca
    13261 cacggatttt gccgctgccg cccgccttag ccggtcattg atcgcttctc ccaggccgct
    13321 ttcggggatc ggggctaccg caatggccga agcgcgtaat ttgtcgagat ttctcagaaa
    13381 agtgtacaag tgcgttgccg cttcggtaag gttagcgctt cgtgacaggt tctgcacaat
    13441 atgcgccttg tccgctccgc tcggcagggc cggaccgaag gccagcagcg cctctccggg
    13501 ggaaacggac gttgcattca gccgcagggg cgtttcggga gcgtagtggg cggtcagccg
    13561 tcccggcgac tgcggcgtct ggttgtcgtc ggtttgaacc ggatcgcgca ccggctctcc
    13621 gagactgctg gacaggtccg cgcgcgtgat tccgccgggc cgaagcagca ccgcttcgtt
    13681 gccgagacag ccgacgatgg tcgattcgac gcccaccgga cagggcccgg ccatcacaac
    13741 cgcatcgatg acaccatcaa gatcagccag cacatgctcg ggcaatgtcg gggagacgtg
    13801 cccggagcgg ttggcgctgg gcgcggccac tggccggccg gccgccttca gcagatcgag
    13861 cgcgacagga tgggagggga tgcgcaatcc cacgcttgcc agccccgcac aggcaagatc
    13921 gcagaccttg cctccctcga tccgggggac caccaaagtc agtggccccg gccagaactg
    13981 cctagccagc cggagggcgg ccgcatccag agtgccctcc gcttgcgccg cttcaagcgt
    14041 cgccagatgg gcgatcagcg gattgaacgc cggccttccc tttgcggcat agatccgggc
    14101 caccgctgcc ggattggttg catccgcgcc aagtccgtag acggtctcgg tcgggagcgc
    14161 gaccagcccg cccgcgcgga tcaggcgcgc agcctcggca atgccggcag gatcgggggg
    14221 aaggcgcatg gtcatgcctt tcccgtagat cacagacaag gctcgggcaa tggtcgcctc
    14281 cggtccatca ggcgaaagtt gtcccttccc gattgcgggg cgctctggcc ggagccgatc
    14341 agattgttta caacgaaccg gttttcggga ggattccatg agctatcgcg cgccggttga
    14401 cgacattctt tctgccatga tccatgcggg agggctcggc aggggcatca cgtccggcat
    14461 ctatgcggat ttgggcgacg gagtggccga ggcgatcctc gaggaagccg gcaagttcgc
    14521 cgccgatcgc cttgcaccgc tcaacaaggt cggcgaccgt catggcacgc cgttaaagga
    14581 cggtcgcgtg accatgccgc cgggctggct ggaggcctat cgtgactggg cggcggccgg
    14641 ctggtgctcg atcgtggcca gtcccgattt cggcgggcag gggctgccga cgctgctcaa
    14701 ttccgcctgc acggaaatct ggaattccgc caatatgggc ttctcgctca atccgctgct
    14761 gacgatgggc gcggtcgagg cgctgacggc gcatggttcg gatacgctga aagagaccta
    14821 tctgacgcgc atgatcgccg gcgaatggac cggcacgatg aatctcaccg agccgggcgc
    14881 cggttccgat ctggcgcaga tccgcacccg cgccgagcgg gcgggcgatg gcagctatcg
    14941 catcaccggt tcgaagatct tcatcaccta tggcgagcat gacctgaccg agaacatcct
    15001 gcatctggtg ctggcgcgtc tgcccgacgc gccggccggc acgcgcggca tctcgctgtt
    15061 cctggtcccc aagtttctcg tcaatgctga cggctcgctc ggcgcccgca atgatgtgcg
    15121 ctgccactcg atcgagcaca agatgggcat tcatgcctcg cccacctgca ccatggtgtt
    15181 cggcgacaat ggcggggcgg tcggctatct ggtcggggag gagaaccgcg gcctggcctg
    15241 catgttcacg atgatgaaca acgcccgcct caatgtcggc ctgcagggtg ttgccatcgc
    15301 cgagcgggcc tatcagcagg cgctcgccta tgcgcaggag cgcaagcagg gcaaggccat
    15361 cggctcgacc gccgccgggc cggaggcgat catcgcccat ccggatgtca agcgcatgct
    15421 gctcgacatg cgcaccaaga cggctgccgc ccgtgcgctg tgctatgcca cggccggcgc
    15481 gctcgaccgg gccgagcgcc tgaccgatcc tgctgcgaag aaggcggcgc atgaggaggc
    15541 cagcctgctg acgccgatcg ccaaggcatt ttcgaccgat atcggcatcg aggttgcctc
    15601 gaccggcgtg cagatccatg gcggcatggg cttcatcgag gaaacgggcg cagcccagca
    15661 tctgcgcgat gcgcgcatcg ccgccatcta tgaaggcacc aacggcattc aggccatcga
    15721 tcttgtcacc cgcaagctgc cgcttgccga tggcgagacg gtgaaagacg tcatccacgc
    15781 ctgccgcacc gccgccgaca aggcggcggc gatcaacgac cccggttttg cccgcatcgg
    15841 ccgtatcctg cgcgatgctg tcggctcgct cgaacgctgc acggatttca tgatgaaggc
    15901 gctgaaggcg gcgccgaacg atgctttggc gggcgcaacg ccctatctcc ggctg
//
LOCUS       DAOMXG010000039        15799 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_2077_length_15799_cov_10.251080, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000039 DAOMXG010000000
VERSION     DAOMXG010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 15799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15799
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2077_length_15799_cov_10.251080"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="PLQ11_04320"
     CDS             <1..443
                     /locus_tag="PLQ11_04320"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HRK24159.1"
                     /translation="ELPEPARAAADIAYLVPDPANELACVLLDHASAAMDISDGLVGD
                     AGKLAAASGCGLIIDTRLVPFPAGIAAVGDCHPALVELCITGGDDYQVLAAIPPHRSA
                     SFEQAARKAGRPVTRIGALTAAGTRIIGRDGRDLTLTRGSFSHF"
     gene            complement(448..963)
                     /locus_tag="PLQ11_04325"
     CDS             complement(448..963)
                     /locus_tag="PLQ11_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HRK24160.1"
                     /translation="MRATLFPVIVGLTVLAGCQQTVKVESSFDPKAAEYIHKAGEGRI
                     DGHAFLKKPNGYVMHASGDVIYLIPATPYAKERMQRLYGASKFMPATRINQAETTDPR
                     YLDFTRRTKAESNGRFTFDKVAPGEYFIVTTLTWKDNENDILSRGGSIYETVTVTGKE
                     DKIIRVVVNGI"
     gene            complement(1052..1714)
                     /locus_tag="PLQ11_04330"
     CDS             complement(1052..1714)
                     /locus_tag="PLQ11_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRK24161.1"
                     /translation="MTATLLFDLDGTLAETDWAHLKAFEIVLGDHGIDIDEAIFKARV
                     VGRSNLEIGAHFFPDMTAAEQKAICDHKEAVYREIVGRIEPVPGLLALLDWADRHDIR
                     CGVVTNAPRLNADHILVGLGLADRFASVVCGQELPESKPHPLPYLTGLAELGGDAAIS
                     VAFEDSPSGLSAAVAAGLPVIGMTTNLAAAEVLAAGATMAASDFTDAAMLAFVRARTG
                     RQ"
     gene            1794..2993
                     /locus_tag="PLQ11_04335"
     CDS             1794..2993
                     /locus_tag="PLQ11_04335"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRK24162.1"
                     /translation="MQNFDTHEAGDRLARRNALVLALSMVLAGANASVIVATGAILGS
                     MLAPSPKLATLPVSAMVLGTAIGTFPASHFMKHVGRRAGFMTGGLLGVIGGLTGAFAI
                     WLGSFWLFTFSAVVSGLYQAFIVLYRYAAADTASPEFRAKAISWVMVGGALSSLVGPQ
                     LVIATQSMLPPLTFLATYLAQAAFAVLAIIVTSRLIDGPLVTAEQAGAARPLMTILKD
                     RRLPAAILTGMVAQAMMNMVMTATPIAMLGCGFSVTQSTLGIQWHILGMYLPSFFTGH
                     LINRFGKGPMMLAGLAMLAGAGGINLAGTALANFWGSLIVIGIGWNFAFVAATALVTD
                     CHAPAERAKVQGFTDLMIFGTTTIASLLAGWLYASFGWSSVNVLLLPVTALAAVAVLT
                     AGLQRRP"
     gene            3163..5316
                     /locus_tag="PLQ11_04340"
     CDS             3163..5316
                     /locus_tag="PLQ11_04340"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019857622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HRK24163.1"
                     /translation="MTALWIIILLGALSVVYAFVTYNQVMSSDAGNARMQEISGAVAE
                     GAQAYLRRQYITIAFVGAAIFVLAWLLLGLPVAIGFLIGAVLSGAAGFIGMNVSVRSN
                     VRTAAAAIKSLAGGLDIAFKAGAVTGMLVAGLALLGVAIYFAILVGMGHAPNSRIVID
                     ALVALGFGASLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPATIADNVGD
                     NVGDCAGMAADLFETYAVTTVATMVLAAIFFGGQPGLWATMVYPLAICAACIVTSIVG
                     TYFVKLGPNNNIMGALYKGLIATGVLSVAGLAIATQVVTGWGAIAQVGNLTITGSALF
                     WCGIVGLVVTALIVVITEYYTGTGKRPVVSIAQASVTGHGTNVIQGLAVSLESTAMPA
                     MVIVGGIIVTFQLAGLFGTAIAATTMLGLAGMIVALDAFGPVTDNAGGIAEMAGLPKE
                     VRHSTDALDAVGNTTKAVTKGYAIGSAGLGALVLFAAYAYDLNYFITAANAPGSTMFP
                     YFKNIGMVSFDLSNPYVVAGLIFGGLIPYLFGGIAMTAVGKAAGSVVEEVRRQFREMP
                     GIMEGKQKPDYARAVDLLTRAAIKEMIIPSLLPVLAPVIAYFAVLAISGSKASAFAAL
                     GASLLGVIVNGLFVAVSMTSGGGAWDNAKKSFEDGFVDKDGVKHLKGSDAHKASVTGD
                     TVGDPYKDTAGPAVNPAIKITNIVALLLLAILAHF"
     gene            5471..6217
                     /locus_tag="PLQ11_04345"
     CDS             5471..6217
                     /locus_tag="PLQ11_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRK24164.1"
                     /translation="MPGRLKGKTAVVTAAGQGIGRAIAEAFVAEGATVYATDRDVEKL
                     EGLARAKRRKLDVRSTRAVDAFADKVGAIDILVNAAGFVHHGSVLECTEADWDFSFDL
                     NVKSMHRTIAAFLPGMLAKGGGSIVNIASGAGSVRGIPNRYVYGATKAAVIGLTKAVA
                     ADFIKKGIRANAICPGTIQSPSLDDRIAALAKLNGITPEAARQAFIDRQPMGRLGTPE
                     EIAWLAVYLASDEASYTTGQIHLADGGFAL"
     gene            complement(6245..7285)
                     /locus_tag="PLQ11_04350"
     CDS             complement(6245..7285)
                     /locus_tag="PLQ11_04350"
                     /inference="COORDINATES: protein motif:HMM:NF016999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HRK24165.1"
                     /translation="MNDRARNWLATGLTLTIATASGALFAAAGLPVPWLSGPMVAVGA
                     LAIAGHRVAMARQVRDLGFLVAGTALGSTVNHEALALLARYPVSIIGLAAGVIAVVAA
                     TTLLLQRRHHWDRTTAFLASIPGALSVVMAVAAESNADERRVVVVQSVRLFVLMVVLP
                     FVIMLAPGVSAAVPRSVTSLPELAALFAASTALALVFVWLRVVSALFLGGMVASAGLH
                     LSGIIPDAVPDLVMQSGALVIGAYCGVRFVGTTGAQLVALLRPSLEALVITLGLSLIA
                     ALLVHALTGINLAAVLVAFAPGGLEAMVLMGAAMGLDTLYISTHHTVRIIGLMLATPV
                     VTPRSSLTDPAE"
     gene            complement(7282..8472)
                     /locus_tag="PLQ11_04355"
     CDS             complement(7282..8472)
                     /locus_tag="PLQ11_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRK24166.1"
                     /translation="MNSNEPRHWPVSQTASNAPSSGIVEVFNYGRNKPGLLPLWVGEG
                     DRPTPAFIHEAAQRSLRDGETFYTYQRGLPELRQAIAAYMSQLYRQELEAERFFVTMG
                     GMHALVMAMRLVTDAGDEVIVPTPAWPNFAGATTVNGARPVEVPMQLGPAGYSLDIDA
                     LAAAITPRTRAICLNSPSNPTGWTATRAEQQAVLALGRRHGLWIIADEIYGRFYYDGA
                     LAPSFHDIMAADDRILFVQTFSKNWAMTGLRLGWLEAPPALGPTIENLIQYTTSGVPV
                     PLQRAGIAALSQGEGLVAELIGQARANRALLLDTLSGLNRIILPRPSGAFYLFFGIEG
                     LPDSRMAALRLVDEALVGLAPGSAFGASGEGFMRLCYLRKGSDVAEAASRLKRWIAAL
                     EDGA"
     gene            complement(8584..9024)
                     /gene="mscL"
                     /locus_tag="PLQ11_04360"
     CDS             complement(8584..9024)
                     /gene="mscL"
                     /locus_tag="PLQ11_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012454486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HRK24167.1"
                     /translation="MLKEFKEFALKGNVVDLAIGVVIGGAFGKIVDSLVADIIMPIIG
                     AITGGIDFSNLMLSLSAGASKAIQGGAGYAKVKEAGAVLGYGQFVTVAVNFLIIAWVL
                     FMVVKALNKLKKAEAAPAPAEPAAPPADITLLTEIRDLLATKKK"
     gene            9345..12944
                     /locus_tag="PLQ11_04365"
     CDS             9345..12944
                     /locus_tag="PLQ11_04365"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012030146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing hybrid sensor histidine
                     kinase/response regulator"
                     /protein_id="HRK24168.1"
                     /translation="MIAGWSIVVWVLFYLCALFIIAHFGDQSGQKLLSGRLRPIFYAL
                     TLAVYCTSWTFLGSVGLASRSGYDFLPIYIGPILVFVFGWRILMRIVKIGKSQNTTSL
                     ADMIASRYGKSEAVAGVVTIICLLGIVPYIALQLKAVALTLETISVPGLRFDRSAPSA
                     LPFGDSALWVTLIMAFFAMVFGTRNTDATKHQTGLVLAVASESLIKLVAFLAVGIFVV
                     WGVFGGLGELFGRAFEDPTIRYKLVFNVDPSTWITTTVIAAFAAVLLPRQFHVLVVEN
                     RELGDIRRAAWLFPLYLVAINLFVVPIAVAGLLLLPAKTDYDLTVLALPILQQSASMT
                     VVAVVGALSAATAMVIVETVALGVMVSNDLVIPFLLKRNTRLREARLSATSDGMMVAQ
                     SRQPTTRFDGGWLQDMGGLVLVIRRIAIAVILLLGYFYYRASSDAALASIGLLSFAAV
                     SQLAPAFFGGLIWHGANARGAVAGPVVGMVCWFVLLFLPSLAGQEGALANWMRSGLFG
                     IDWLRPASLVIGDLSALVSGVIISLSLNILSYIGLSLTRAATPVERLQANVFIGTDPG
                     LTGTSFRLWRSAVTMTELKTTVARYLGEERTTREFNEFATRQGIEPMPELEADAHTIR
                     FAEHLLASVIGAASSRLVLSLMLRRRNVSTEAALKLLDDASAAIQYSRTLLQHALDHT
                     EQGVTVFDSDLRLVCWNQAFKDLFDLPSDMVRFGVGLDDIVGYNAKRGTYGADMSSDS
                     IARRVQAFVTRTEPERLRLHAPSRWLEVRSNHLPDGGIVTTYTDITVAVKAEAALNQA
                     NETLERRVLQRTNELTRVNEELGRAKAEAEQANLSKTRFLAAASHDILQPLNAARLYA
                     TSLNERSEGSELGSLARNVGASLDSVEEILTALLEISRLDSGKLKPELSTFRIDEMFG
                     QLAVEFAPLARQKNLTLTFVPSRLSVRSDRRLLRRILQNLISNAIKYTPEGRVLVGCR
                     RRADQVRIEVRDTGIGVPEGKRTAIFKEFQRLDEGAKAARGLGLGLSIVDRIARALGH
                     PLSVGASGRRGSVFSVDIPVVSVGIYAVTPAEPMVLQAPPLAGLSVLAIDNEPTILDG
                     LRILLEGWGCKVLIASDYDSARRLIKAAPRKPDMIIADFHLDNDNGIAVVRRLRSRFR
                     SAIPAVLITADRSPEVRDKAAEYDLVLLNKPLKPAALRALLTQTRAALEIAAE"
     gene            12983..13342
                     /locus_tag="PLQ11_04370"
     CDS             12983..13342
                     /locus_tag="PLQ11_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24169.1"
                     /translation="MIRSPGPIRDAVRLLLAMTLLVAGVAFARVQGEHSGLALLLGGN
                     AHACLSTADGLVDSAGGSESLHCPACMLAAGHLAPEPIGPALPIRWVASDFVQGTDLE
                     RQQMVPTVRARGPPGTV"
     gene            13384..13863
                     /locus_tag="PLQ11_04375"
     CDS             13384..13863
                     /locus_tag="PLQ11_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012496368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HRK24170.1"
                     /translation="MQKFILKAGIGLAFSLVTGLALAQSTTSSVLVNQPWARATPPGA
                     KVAGGFMVLENRSGAADRLIAATADVSGVVEIHEMAMENGVMKMRALDQGLPLNAGAR
                     VELKPGSYHIMFIDLKKPLVAGETVRGELVFEKAGRMPVEYKVMPIGNSQPGGHNHH"
     gene            14029..14622
                     /locus_tag="PLQ11_04380"
     CDS             14029..14622
                     /locus_tag="PLQ11_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031966 - mitochondrial membrane
                     [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial cytochrome c
                     oxidase assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HRK24171.1"
                     /translation="MSTPNTALRLIRWVSWSLIGLLGAVTLYILLQPEQQRVSLGGPF
                     TLTTMEGERRNVGTHEKGRPYAVFFGFTYCPDICPTTMFELSQTLKAAGDAVKDFRVY
                     FVSVDPERDTPEQLKLYLSAFDSHIVGLTGTPEEIAAVARQHRAFYRKVPTPSSYTMD
                     HTATIFLFDRKGSFFGTLDVNEPESARLAKLQRLMRS"
     gene            complement(14629..15249)
                     /locus_tag="PLQ11_04385"
     CDS             complement(14629..15249)
                     /locus_tag="PLQ11_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HRK24172.1"
                     /translation="MARNIDYYFAIYSPWSYIGHKPFIEIATRHRLPITYKPTPLLEV
                     FAETGGVPLAQRPPARQSYRWVELQRWRDKRGLSLNLKPKFWPYKSTLADRCVIAVQQ
                     TEHDPDTFLTLAYKAVWSKDLNLDDESLVASLLSASGLDPGRIIHLANSEAVQARYRQ
                     NKDDAVAAGVFGAPSYVIEGEIFWGQDRLDLLSDMLASGRKPYRPL"
     gene            complement(15293..>15799)
                     /locus_tag="PLQ11_04390"
     CDS             complement(15293..>15799)
                     /locus_tag="PLQ11_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRK24173.1"
                     /translation="ERLEPPFGVSRLPFRVLPNDLDTNLHLNNGRYLTIADLGRTDLL
                     LRSRLWRAVIEKGWMPMLSGGIVRFRRELRPFEAFTLETRVVYWNERNMVMEHRFITR
                     RRGKGPDQVAAIVLVRGGLYDRTQGRFIDCAEIFRFAGYEGPSPEVTPEVAAFMAAED
                     QLKRMAVG"
BASE COUNT         2614 a         5099 c         5025 g         3061 t
ORIGIN      
        1 ccgagctgcc cgagccggcg cgggcggcgg cggacattgc ctatcttgtg cccgacccgg
       61 ccaatgaact cgcttgcgtt cttctcgatc acgccagcgc cgcgatggac atatccgacg
      121 ggctggtggg cgatgccggc aagctggcgg cggccagcgg ctgcgggctg atcatcgaca
      181 cgcgtctggt gccgtttccg gccggtatcg ccgcagtcgg cgattgccac ccggcgctgg
      241 tcgaactctg catcaccggc ggcgacgact atcaggtgct tgccgcgatc ccgccgcacc
      301 gcagcgccag ctttgaacag gcggctcgca aggccggtcg tcctgtaacg cgcatcggcg
      361 ccctgacggc agcgggaacc cgcatcatcg gccgcgacgg ccgcgaccta accctcacgc
      421 gcgggtcatt cagccacttc tgatccgtca gattccgttc acgacgacgc ggatgatctt
      481 gtcttccttg ccggtgacgg tgactgtctc gtagatcgat ccgccgcgcg acaggatgtc
      541 gttttcattg tccttccagg tcagggtggt gacgatgaaa tactcgccgg gggccacctt
      601 gtcgaaggtg aaacgcccgt tggactcggc cttggtcctg cgggtgaagt cgagatagcg
      661 cggatcggtc gtctcggcct gattgatgcg ggttgcgggc atgaatttgg atgcgccata
      721 caggcgctgc atgcgttcct tcgcataggg tgttgccgga ataaggtaga tgacatcgcc
      781 cgaggcgtgc atcacatagc cgttcggctt tttcagaaac gcatgaccgt cgatgcggcc
      841 ctcgcccgct ttgtggatgt attcggctgc cttgggatcg aagctgctct cgaccttcac
      901 cgtctgctgg cagccggcca gaacggtcag ccccacaatc actggaaaca acgttgcgcg
      961 catgacctca ccttatgtca gggcatcatt gcacgtcgct gccgtcgtgc aatgcgcttc
     1021 ctgcgcgggt gcgacgtctt ggccggtccg gtcactgccg gccggtgcgg gccctgacga
     1081 acgccagcat ggcggcgtcg gtgaagtcgc ttgcggccat ggtagctcct gcggcgagca
     1141 cctcggcggc cgcaaggttg gtcgtcatgc caatgaccgg caggccggcc gcgaccgctg
     1201 cgctgaggcc ggaagggctg tcctcgaagg caacggatat ggcggcatcg ccgccgagct
     1261 cggcaaggcc ggtgaggtag ggcaggggat gcggcttgct ctccggcagt tcctgcccgc
     1321 aaaccaccga ggcgaagcga tcggcgagac ccagcccgac aagaatgtgg tcggcattga
     1381 gccgcggggc attggtcacc accccgcagc ggatgtcgtg ccggtcggcc cagtcaagca
     1441 gcgccagaag acccgggacc ggttcgatcc tgccgacaat ctcgcgatag accgcttcct
     1501 tgtggtcgca gatggccttc tgttcggctg cggtcatatc cggaaagaaa tgtgcaccga
     1561 tctcgaggtt cgaccgaccg accaccctgg ccttgaagat cgcctcgtcg atgtcgatgc
     1621 catgatcgcc cagcacgatc tcgaaagcct tgagatgggc ccagtcggtt tcggccaggg
     1681 ttccgtcaag gtcgaacagc agcgttgcgg tcatgaaatt cttttctccg gcagcgggtt
     1741 cctgctggtg tcgacgccgc taccgggtta gtcaactgtc aagcaggccc ttgatgcaga
     1801 attttgatac ccacgaagcg ggcgaccggc tcgcccgacg caatgccctc gttctggcgc
     1861 tcagcatggt gcttgccggc gccaatgcct cggtcatcgt ggccaccggc gccattctcg
     1921 gctcgatgct ggcgccgtca ccgaagctgg cgacgctgcc ggtctctgca atggtgctgg
     1981 gtaccgcgat cgggaccttt ccggcctccc atttcatgaa gcatgtcgga cgccgggccg
     2041 gcttcatgac cggcggcctg ctcggggtga tcggcgggtt gaccggggcc ttcgccatct
     2101 ggctgggctc gttctggctg ttcacctttt cggcggtcgt ctccggcctc taccaggcct
     2161 tcatcgtgct ctatcgctat gcggcggccg ataccgcttc gccggaattc cgcgccaagg
     2221 cgatttcctg ggtgatggtc ggtggtgccc tgtcatcgct ggttggtccg cagctggtga
     2281 tcgccaccca gtccatgctg ccgccgctga cgtttctggc cacctatctg gcgcaggctg
     2341 cctttgccgt gctggcgatc atcgtcacat cgcggctgat cgatggcccg ctggtgacgg
     2401 ccgaacaggc cggtgcggcc cggcccctga tgacgatcct gaaggatcgc cggctgcctg
     2461 cggcgattct caccggcatg gtcgcgcagg caatgatgaa catggtgatg acggcgacgc
     2521 cgatcgccat gctgggatgc ggcttttcgg tgacgcaatc gacgctgggc atccagtggc
     2581 atatcctcgg catgtatctg ccgagtttct tcaccggaca ccttatcaac cgcttcggca
     2641 agggcccgat gatgctggcc ggtctggcga tgctggccgg cgccggcggc atcaacctgg
     2701 ctggaaccgc actggccaat ttctggggtt cgctgatcgt tatcggcatc ggctggaact
     2761 ttgccttcgt tgccgcaacc gcgctggtca ccgattgcca tgcgccggcc gagcgcgcca
     2821 aggtgcaggg cttcaccgat ctgatgatct tcggcacgac gacgatcgcc tcgctgctcg
     2881 ccggctggct ctatgcctcg ttcggttggt cgtcggtgaa tgtgctgctg ttgccggtca
     2941 ccgcgctggc cgccgtcgct gtcctgacgg ccggtttgca gcgcaggcca tgaactttcg
     3001 tcgcgtgcga ccaaaaagct atgggtaatt agactgaatt tgcctcgcag cgggcatttt
     3061 cgctttggtg gttgattaac catttgctaa aggcggttcg agttccgtcc gggctggggt
     3121 tttgaggcgc gggcggacca ccaatcaata cgggacacaa tcatgaccgc tctctggatc
     3181 attatcctcc tcggcgcgct gtctgtcgtc tatgccttcg tcacctacaa ccaggtgatg
     3241 agctcggacg ctggcaatgc ccgcatgcag gagatttcgg gtgctgtcgc ggaaggcgct
     3301 caggcctatc tccgccggca gtacatcacc atcgccttcg tcggcgcagc cattttcgtg
     3361 ctggcctggc tgcttctcgg ccttcctgtg gcgatcggct tcctgatcgg cgccgtgctt
     3421 tcgggcgcgg ccggcttcat tggcatgaac gtgtcggtgc gctccaacgt gcgcacggcc
     3481 gccgccgcga tcaagagcct tgccggcggc ctcgatatcg ccttcaaggc gggcgcggtc
     3541 accggcatgc tggtggcggg cctggcgctg ctgggcgtgg cgatctactt cgcgattctg
     3601 gtcggcatgg gtcatgcgcc caacagccgc atcgtcatcg acgcgctggt ggcgctcggc
     3661 ttcggcgctt cgctgatctc gatctttgcc cgtcttggcg gcggcatctt caccaagggt
     3721 gcggacgtcg gcgctgacct cgtcggcaag gtcgaggccg gcatcccgga ggatgatccg
     3781 cgcaacccgg cgacgattgc cgacaacgtc ggcgacaacg tcggcgactg cgccggcatg
     3841 gcggctgacc tgttcgagac ctatgcggtg accacggtcg ccaccatggt tctggcggcg
     3901 atcttcttcg gtggccagcc cggtctgtgg gcgacgatgg tctatccgct ggcgatctgc
     3961 gcggcctgta tcgtcacctc gatcgtcggc acctacttcg tcaagctcgg cccgaacaac
     4021 aacatcatgg gcgcgctcta caagggcctg atcgccaccg gcgtgctgtc ggttgccggt
     4081 ctggcgattg ccacccaggt cgtcaccggc tggggcgcga ttgcccaggt tggcaacctg
     4141 acgatcaccg gctcggcgct gttctggtgc ggtatcgtcg gcctggtcgt caccgcgctg
     4201 atcgtggtga tcaccgagta ttacaccggc acgggcaagc gcccggtggt gtcgattgcc
     4261 caggcgtcgg tcaccggtca cggcaccaac gtcatccagg gcctcgcggt ctcgctggaa
     4321 tccaccgcga tgccggccat ggtgatcgtc ggcggcatca tcgtcacctt ccagctcgcc
     4381 ggcctgttcg gcacggcgat cgcggcgacc accatgcttg gcctcgccgg catgatcgtt
     4441 gcgctcgacg ctttcggccc ggtgaccgac aacgccggcg gcattgccga aatggcgggc
     4501 ctgcccaagg aagtgcgtca ttcgaccgac gcgctcgacg cggtcggcaa caccaccaag
     4561 gcggtgacca agggctatgc catcggctct gccggacttg gcgcgctggt gttgtttgcg
     4621 gcctatgcct atgatctcaa ctacttcatc accgcggcga atgccccggg ttcgacgatg
     4681 ttcccctatt tcaagaacat cggcatggtc tcgttcgacc tgtcgaaccc ctacgtcgtc
     4741 gccggcctga tcttcggcgg tctcatcccc tacctcttcg gcggtatcgc gatgacggcg
     4801 gtcggcaagg cggccggttc ggtggtggaa gaggtccgtc gccagttccg ggaaatgccg
     4861 ggcatcatgg aaggcaagca gaagccggac tatgcccgtg ccgttgacct gctgacccgc
     4921 gcggcgatca aggagatgat catcccctcg ctgctgccgg ttctggctcc ggtcatcgcc
     4981 tatttcgcgg tgctggcgat ctccggctcc aaggcctcgg ccttcgcggc gctcggcgcc
     5041 tcgctgctcg gcgtgatcgt caacggcctg ttcgtcgcgg tctcgatgac ctccggcggc
     5101 ggcgcctggg acaacgccaa gaagtcgttc gaggatggct tcgttgacaa ggacggcgtc
     5161 aagcacctca agggctccga tgcgcacaag gcgtcggtga ccggtgatac cgtcggcgac
     5221 ccctacaagg acaccgccgg cccggccgtc aacccggcga tcaagatcac caacatcgtg
     5281 gcgctgctgc tgctggcgat cctggcccac ttctgagaac cgatcacctt cgggtgacgc
     5341 tgaagggcgg gggaaacccc gccctttttg ctgggcggct gcggctgcat ggtgacgcat
     5401 gccaggccgc ttgcattcgg cggcaaagtg tccgatcact ttgccggtca tcagacaact
     5461 ggagtgcatc atgccgggcc gtctcaaggg aaaaaccgct gttgtcaccg ctgcggggca
     5521 ggggatcggc cgtgccatcg ccgaagcctt cgttgccgag ggcgcgacgg tttatgccac
     5581 cgatcgcgat gttgaaaagc tcgaggggct ggcgcgggcc aagcgccgca agctcgatgt
     5641 gcgctccacc cgcgcggtcg atgcttttgc cgacaaggtc ggcgccatcg acattctggt
     5701 caatgccgcc ggcttcgtgc atcacggctc ggtgctcgaa tgcaccgagg cggactggga
     5761 tttttcgttc gatctcaacg tcaagtcgat gcatcgcacc atcgccgcct tcctgccggg
     5821 catgctggcc aagggcggcg gttcgatcgt caatattgca tccggcgccg gctcggtgcg
     5881 cggcatcccg aaccgctatg tctatggcgc gaccaaggcg gcggtgatcg gcctgaccaa
     5941 ggccgtggcg gcggatttca tcaagaaggg catccgcgcc aatgccatct gccccggcac
     6001 catccagtcg ccctcgctcg acgaccgcat cgccgcgctg gcgaagctga acggcatcac
     6061 accggaggcg gcgcggcagg ccttcatcga tcgccagccg atgggccggc tcggcacgcc
     6121 cgaggaaatc gcctggctgg cggtctatct ggcctccgac gaggcgagct acaccaccgg
     6181 tcagatccac ctcgcggatg gcggcttcgc gctgtgatcc agccgcggct ggtgcctgac
     6241 ggctctattc cgccggatcg gtgaggctgg accggggcgt caccaccggc gttgccagca
     6301 tcagcccgat gatccgcacg gtatggtggg tcgaaatata gagcgtatcg agccccatcg
     6361 ccgcccccat cagcaccatg gcttcaaggc cgccgggcgc aaaggccacc aatactgcgg
     6421 cgaggtttat cccggtcaaa gcatgcacca gcagggcagc gatcagcgac aggccgagcg
     6481 tgatcaccag tgcttccagc gacggacgca gcagcgccac cagttgcgct cctgtggtgc
     6541 cgacaaagcg cacgccgcaa taggcgccaa tcaccagtgc cccgctctgc atgaccagat
     6601 cgggcacggc atcggggatg atgccggaca ggtgcaggcc ggcgctggcc accatgccgc
     6661 ccagaaacag cgcgctcacc acccgtagcc agacgaacac cagcgccagt gcggtcgagg
     6721 cggcgaacag ggcggccagt tccggcaggc tggtgaccga gcgcggcacc gccgcagaaa
     6781 ccccgggcgc cagcatgatg acaaacggca gcaccaccat cagcacaaac aggcggaccg
     6841 attgcaccac cacgacgcgc cgctcgtcgg cgttgctttc ggccgctacc gccatcacca
     6901 ccgaaagggc gccgggaatg gaggccagga acgcggtcgt ccgatcccag tgatggcggc
     6961 gttgcagcag aagggttgtc gcggcaacca cggcgatgac gcctgcggca agtccgatga
     7021 tgcttaccgg atagcgtgcc agcagcgcca gggcctcatg attgacggtc gagcccagcg
     7081 cagtgccggc gaccagaaag cccagatcgc gcacctggcg cgccatcgcg actcgatgac
     7141 cggcaatcgc cagcgcgcca acggcaacca tcggtccgga gagccagggc acgggcaggc
     7201 cggctgcggc gaacagggcg ccgcttgctg tcgcaatagt gagtgtcaga ccggttgcga
     7261 gccagtttcg cgcgcggtca ttcaagcccc gtcctccaga gcagcgatcc agcgcttcag
     7321 ccgactggcc gcttcggcca catcgctgcc cttgcgcaga tagcacagcc gcatgaagcc
     7381 ttcgccgctg gcgccgaagg cggagcctgg cgccagaccg accagcgcct cgtcgaccag
     7441 cctgagggcc gccatccggc tgtcgggcaa tccctcgatg ccgaagaaca ggtagaaggc
     7501 gccggagggg cgcggcagga tgatccggtt gaggccggag agcgtatcga gcaacagggc
     7561 gcggttggcc cgcgcctggc cgatcagttc ggccaccagc ccttcgccct ggctcaacgc
     7621 ggcgatcccc gcccgctgca acggcaccgg cacgcccgaa gtggtgtatt ggatgaggtt
     7681 ttcgatggtc gggccgaggg cgggcggcgc ttccagccag ccaagcctga gcccggtcat
     7741 cgcccagttc ttggagaagg tctggacgaa caggatccgg tcgtccgccg ccatgatgtc
     7801 gtgaaatgaa ggcgccagcg cgccatcata gtaaaagcgt ccgtagatct cgtcggcaat
     7861 gatccacagg ccgtggcgcc gccccagcgc cagaacggcc tgctgttcgg cgcgcgtggc
     7921 ggtccatccg gtcgggttgg atggcgaatt caggcagatg gcgcgtgtgc gaggggtgat
     7981 ggcagcggca agagcgtcga tatcgaggct gtagccggcc gggccgagtt gcatgggcac
     8041 ttcgaccggg cgggcgccgt tgaccgttgt ggcgccggca aaattcggcc aggcgggggt
     8101 cggcacgatc acctcgtcgc cggcgtcggt gaccaggcgc atcgccatga ccagcgcatg
     8161 cattccgccc atcgttacga aaaatcgttc tgcctcaagc tcctgccggt agagttggct
     8221 catgtaggcg gcaatcgcct ggcgcagttc gggcaggccg cgttggtagg tatagaaggt
     8281 ctcgccatcg cgtaatgagc gctgcgccgc ctcgtgaatg aaggccggcg tcggccggtc
     8341 tccctcgccc acccaaagcg gcagcaggcc gggtttgttg cggccataat tgaaaacttc
     8401 gacgatgccg ctcgacggcg cattgctggc ggtctggctg accggccagt gtcttggctc
     8461 attggagttc atcgccggat tctggcgatc cgctctcgaa aaggcaatgc gtttcttagc
     8521 gatcaaacaa aaacctcccg gctgtccgcc gggaggttct tcagcgccag cctgtggcgg
     8581 cgattacttc ttcttcgtgg cgagcaggtc gcggatctcg gtcagcagcg tgatatccgc
     8641 aggcggagct gcaggttccg ccggagccgg cgccgcttcg gccttcttga gcttgttgag
     8701 ggccttgacg accatgaaca gcacccaggc gatgatcagg aagttgaccg cgaccgtcac
     8761 gaactggcca tagccgagca cagcgccggc ctccttgacc ttggcatagc cggcaccacc
     8821 ctggatggcc ttgcttgctc cggccgacag gctgagcatc agattggaaa aatcgatgcc
     8881 gccggtgatc gcgccgatga tcggcatgat gatatccgca accagcgaat caacgatttt
     8941 gccgaaggcg ccgccgatca cgacaccgat ggccagatcg accacattgc ccttgagggc
     9001 aaattccttg aattccttga gcatggcagc cccctaatgt tgacttggca ggatcccagc
     9061 gtatcccccc cctgtcgcta atactctttt ccggcaaatc tgcaactttc gtcatagagc
     9121 gtgctgcgat taggtggaat ccggctaatc gcaacaagca tgctctaagc acttgataac
     9181 gatcatgtta tctctcgttt tgatgtttca atcaaaacga gagcatgatc taggaaggcg
     9241 gcgaattgcc cacagatcgg cccggatcgg ctgccggggc ttgcgattcc tgcggtttgc
     9301 ctgttcgatc acgccatgca gggagccata ctggacggac gcgcatgatt gccggctgga
     9361 gcatcgtcgt ctgggtgctg ttttatctct gcgccctgtt catcatcgcc cattttggcg
     9421 atcagagtgg ccagaagctg ctgtccggcc ggttgcggcc gatcttctat gcgctgaccc
     9481 tggccgtcta ttgcacctcc tggacctttc tcggctcagt cggcctcgcc tcgcgctcgg
     9541 gctatgattt cctgccgatc tatatcgggc cgatcctcgt ctttgtcttc ggctggcgca
     9601 tcctgatgcg catcgtcaag atcggcaaat cgcagaacac cacctcgctc gccgacatga
     9661 tcgcctcccg ctacggcaag agcgaagcgg tggccggcgt ggtgacgatc atctgcctgc
     9721 tggggatcgt gccctatatc gcgctgcagc tcaaagccgt ggcgttgaca ctggaaacga
     9781 tttcagttcc ggggctgcgg tttgaccgct cggcgccgtc ggcgctgccg tttggcgatt
     9841 cggcgctgtg ggtgacgctg atcatggcgt tctttgccat ggtctttggc acacgcaaca
     9901 ccgatgccac caagcaccag accgggcttg tgctggcggt cgccagtgaa tcgctgatca
     9961 agctggtggc gtttctggca gtcggcatct ttgtcgtctg gggcgtcttc ggcgggctgg
    10021 gagagttgtt cggccgtgcc tttgaagatc cgaccatccg ctacaagctg gtgttcaatg
    10081 tcgatccttc gacctggatc accaccacgg tgatcgctgc ctttgctgcg gtcttgctgc
    10141 cgcgccagtt tcatgtgctg gtggtcgaga accgtgaact gggcgatatc cggcgggctg
    10201 cctggctgtt cccgctctac ctggtggcga tcaacctgtt cgtggtgccg atcgcggtgg
    10261 cgggcctgct gctgctgccg gccaagaccg attatgatct caccgtgctg gctctgccga
    10321 tcctgcagca atcggcgtcg atgaccgtcg tcgcagttgt cggggcgctg tcggcggcaa
    10381 ccgcgatggt gatcgtcgaa acggtggcgc tcggcgtcat ggtctccaac gatctggtga
    10441 tcccgttcct gctcaagcgc aacacccggc tgcgcgaggc gcgcctgtcg gcgacaagcg
    10501 acggcatgat ggtggcccag tcgcgtcagc cgacgacccg gttcgatggt ggctggctgc
    10561 aggacatggg cgggctggta ctggtcatcc gtcgcatcgc catcgccgtc attctcctgc
    10621 ttggctattt ctactaccgc gcctcgtccg atgcagcgct tgccagcatc ggtctgctgt
    10681 cctttgcggc ggtctcgcaa ctggcgccgg cgttcttcgg cgggctgata tggcatggcg
    10741 ccaacgcgcg cggcgccgtc gccggtcctg tcgtcggcat ggtctgctgg ttcgtgctgc
    10801 tgtttctgcc gtctctggcc ggtcaggagg gcgcgctggc caattggatg cgctctggcc
    10861 tgttcgggat cgactggctg cgcccggcat cgctggtcat cggcgatctg tcggcgctgg
    10921 tcagtggtgt catcatctcc ctgtcgctca acatcctctc ttacatcggc ctttcgttga
    10981 cgcgcgccgc aacaccggtg gagcggttgc aggccaacgt gttcattggc acggaccccg
    11041 gcctgacggg caccagcttc cgcctgtggc gcagcgcggt caccatgact gaactcaaga
    11101 cgaccgtggc gcgctatctc ggcgaggagc ggacgacgcg cgagttcaac gaatttgcca
    11161 ccaggcaggg cattgagcca atgcctgaac tggaggcgga tgcccatacc atccgcttcg
    11221 ccgagcatct tctggcctcg gtgatcggtg cggcctcctc gcggctggtg ctgtcgctga
    11281 tgctgcggcg ccgcaacgtc tccaccgagg cggcgctgaa gttgctcgat gacgcttcgg
    11341 cggcgatcca gtacagccgg acgctgctcc agcatgcgct cgatcatacc gagcagggcg
    11401 ttaccgtgtt cgacagcgat ctcaggctgg tctgctggaa ccaggcattc aaggacctgt
    11461 tcgatctgcc gtcggacatg gtgcgtttcg gtgtcgggct ggatgatatc gtcggctaca
    11521 atgccaagcg cggcacctat ggcgccgaca tgtcatccga ttccatcgcc cgccgcgttc
    11581 aggccttcgt tacccgcacc gaaccggagc gtctgcgcct gcacgcgccg agccgctggc
    11641 tggaagtccg ctccaatcat cttcctgatg gcggcattgt caccacctac accgacatca
    11701 ccgtggccgt gaaggcggag gcggccctca accaggccaa cgagacgctg gaacgccgcg
    11761 tcttgcagcg caccaacgaa ctgacccgcg tcaacgagga actcggccgg gccaaggccg
    11821 aggccgaaca ggccaacctg tccaagaccc ggtttctggc cgcggccagt cacgacattc
    11881 tccagccgct caacgcggcg cggctctatg ccacctcgct caacgagcgc agcgaaggca
    11941 gcgaacttgg cagccttgcc cgcaatgtcg gcgcttcgct tgattcggtc gaggagatcc
    12001 tgacggcgct gctggaaatt tcgcgcctcg acagcggcaa gctgaagccc gagctgagca
    12061 cattccggat agacgagatg tttggccagc ttgctgtgga atttgctccg ctggcccgcc
    12121 agaagaacct gacgctgacc ttcgtaccat cccggctgtc ggtgcgctct gaccggcggc
    12181 tgttgcggcg cattttgcag aatctgattt ccaacgccat caaatacacg ccggaaggcc
    12241 gcgttctggt cggttgccgg cggcgcgccg accaggttcg catcgaggtg cgtgacaccg
    12301 gaatcggcgt tccggaaggc aagcggacgg cgatcttcaa ggaattccag cgactggacg
    12361 aaggggccaa ggcagcccgc ggcctcggcc tcggcctttc catcgtcgat cgcattgccc
    12421 gtgcgcttgg ccatccgctc agcgtcgggg cgagtggccg gcgcgggtcg gtgttttccg
    12481 ttgatattcc ggtggtctcg gttggcatct atgccgtgac ccccgctgag ccgatggtcc
    12541 ttcaagctcc acccctggcc ggcctctccg tcctggccat cgacaacgag ccaaccattc
    12601 tcgacggttt gcgcatcctg ctggaaggct ggggctgcaa ggttctgatc gccagcgatt
    12661 acgattccgc acgccgtctg atcaaggcgg cgccgcgcaa gccggacatg atcatcgccg
    12721 acttccacct cgacaacgac aacggcatcg ccgtcgtgcg ccggctcaga agccggttcc
    12781 gctcggcaat accggcagtg ctgatcacag ccgaccgctc gcccgaagtg cgcgacaagg
    12841 cggcggaata tgatctggtc ctgctcaaca agccgctgaa gccggcggcg ctccgcgccc
    12901 tgctgaccca gacccgggcc gcgctggaga ttgcggcgga ataacccttt tgcgctgcgg
    12961 ctcgctgcga taagaacggc gaatgatccg ttcgcctgga ccgatacgtg acgctgttcg
    13021 cctgttgctg gcaatgaccc tgctggtggc gggcgtggcg ttcgcccgcg ttcagggtga
    13081 acactccggc cttgcactgc tgctcggcgg caatgcgcat gcctgcctca gcacggcaga
    13141 cggcttggtc gactccgccg gcggatcgga atccctgcat tgccccgcct gcatgctcgc
    13201 agccggccat cttgcgccgg agccgatcgg cccggccttg ccaatccgtt gggtcgcatc
    13261 tgatttcgtc cagggcacag accttgagcg ccagcagatg gtcccgaccg tgcgcgcccg
    13321 tggacctccc ggaaccgtct gaccaacccg ttccgtcttt ggtttccaca tatcgaggct
    13381 atcatgcaga agttcatcct gaaggccggg atcggccttg ccttttcgct tgtcacgggg
    13441 ctggccctcg cccagtccac gacctcctcc gtcctggtca accagccctg ggcgcgggcc
    13501 acgccgccgg gtgccaaggt tgccggcggg ttcatggtcc ttgaaaaccg cagcggtgca
    13561 gccgatcggc tcattgccgc cacggccgat gtttccggcg tggtcgagat ccatgaaatg
    13621 gcgatggaga acggagtgat gaagatgcgc gccctcgacc agggcctgcc gctcaatgcc
    13681 ggcgcgcgcg tcgaactcaa gcccggctcc tatcacatca tgttcatcga cctgaagaag
    13741 ccgcttgtcg ccggggaaac cgtgcgcggc gagcttgtct ttgaaaaggc cggacgaatg
    13801 ccggtcgaat acaaggtcat gccgataggc aacagccagc ccggcggcca taaccatcat
    13861 tgacgactgg agcatgcggc gaaaaagtgt ggacggtttt tcgccaaaag catgctctga
    13921 cttattgtag tcgatcacgc tctcgtttcg gtgatccaac cgaaacgaga gcgtgatctg
    13981 cggcggggag cggcaccgct cccgctggct tttcccggaa ggtgtttgat gagcactccc
    14041 aatactgctc tgcggctgat ccgctgggtg agctggtccc tgatcggcct gctcggcgcg
    14101 gtgacgctct atatcctgct ccagccggaa cagcagcgcg tcagcctcgg cgggcccttc
    14161 actttgacca ccatggaagg cgagcggcgc aatgtcggca cccatgaaaa ggggcggccc
    14221 tatgcggtgt ttttcggctt cacctattgc ccggacatct gcccgacgac aatgttcgaa
    14281 ctgtcgcaga cgctgaaggc ggccggcgat gcggtgaagg atttccgcgt ctattttgtc
    14341 tcggtcgatc ccgagcgcga cacgcccgaa cagttgaaac tctatctctc ggcgttcgat
    14401 tcccacatcg tcggcctgac cggaacgccg gaggagatcg ctgcggtcgc ccgccagcat
    14461 cgcgccttct atcgcaaggt gccgacgcct tcgagctaca cgatggacca cacggcgacg
    14521 atcttcctgt ttgaccgcaa gggcagcttt ttcggcacgc tcgacgtcaa cgagccggaa
    14581 agcgcgcgcc tggccaagct ccagcggctg atgcgatcgt gaggccgctc acagcggccg
    14641 gtagggcttg cgcccgctgg ccagcatgtc gctgagcagg tcgaggcgat cctgccccca
    14701 gaaaatctcg ccctcgatca catagctcgg ggcaccgaat acgccggccg cgacggcatc
    14761 gtccttgttc tggcgatagc gggcctgcac ggcctcgctg ttggcaaggt ggatgatgcg
    14821 gccgggatca agcccgctgg ccgacagcag cgaagcgacc agagactcat cgtccagatt
    14881 gaggtctttc gaccagacgg ccttgtaggc cagcgtcagg aacgtgtcag ggtcatgttc
    14941 ggtttgctgg acggctatca cgcagcgatc tgccagggtt gatttgtagg gccagaactt
    15001 cggcttgaga ttgagcgaca ggccgcgctt gtcgcgccag cgctgcaatt caacccaacg
    15061 gtaactttgg cgtgccggcg gtcgctgcgc cagcggcacg cctccggttt cggcaaacac
    15121 ctccagcagc ggggtcggct tgtaggtgat cggcagccgg tgccgcgtcg cgatctcgat
    15181 gaacggcttg tggccgatgt aggaccacgg gctgtagatg gcaaagtaat aatcgatgtt
    15241 tcttgccata gggcaggtct ccagctcggc tgtcaggccc ggctggaagg catcagccga
    15301 cagccatccg cttcagttga tcttcagccg ccatgaaggc agccacttcg ggcgtcacct
    15361 cgggagatgg cccttcatat cctgcaaagc ggaagatttc ggcgcagtca atgaagcgac
    15421 cttgcgtccg gtcataaagg ccgccgcgca ccagcacgat ggcggcgacc tggtccggac
    15481 ccttgccgcg ccggcgcgtg atgaagcggt gctccatcac catgttgcgc tcgttccagt
    15541 agacgacgcg ggtttcgagc gtgaacgcct cgaacggccg caattcgcgc cggaaccgca
    15601 cgattccgcc cgaaagcatc ggcatccagc ccttctcgat caccgccctc cacagccgcg
    15661 aacgcagtag cagatcggtg cggccaagat cggcgatggt gagataccgc ccgttgttga
    15721 gatgcaggtt ggtgtcgaga tcgttcggca gcacgcggaa cggcagccgg gagacgccga
    15781 acggcggctc cagccgctc
//
LOCUS       DAOMXG010000040        15546 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_2112_length_15546_cov_8.910464, whole genome shotgun sequence.
ACCESSION   DAOMXG010000040 DAOMXG010000000
VERSION     DAOMXG010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 15546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15546
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2112_length_15546_cov_8.910464"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..654
                     /locus_tag="PLQ11_04395"
     CDS             166..654
                     /locus_tag="PLQ11_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24174.1"
                     /translation="MTLHRFMIGFGAFCAMALPAVAQTWQAAPPTGAKLGLKAIGCKG
                     EFCLAVACVGGKPQLVSLSPGGGPFNGATKVTVGPASASVTFVEDPKIMDAFNMLGTR
                     APVMPELIADMATAKDITLAGPTFSDKVTSRFLLNGYAKLAPAIARDCGIKGSGSQPA
                     GE"
     gene            complement(704..1369)
                     /locus_tag="PLQ11_04400"
     CDS             complement(704..1369)
                     /locus_tag="PLQ11_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24175.1"
                     /translation="MSNQLHGKKFEDLLKGCGLFSGAADSKRSPISGFDIEEKFDRLL
                     GIPTSIKATGTNTVSLSDARKFWSINHPFRMLIGVYVQDGVHKKFVRVEEILVHGCMF
                     AGLRGNISAAAVAELHEGIGLAAFDHGEEEEARDWFREVHAKVLHNLGLVRLNPKIDS
                     KGQRRLQCSVPLDSLVALAKKMPDFVGQSGQPAPTYRCHAEEIGDLGLPITLKSGRRV
                     FNP"
     gene            complement(1396..2190)
                     /locus_tag="PLQ11_04405"
     CDS             complement(1396..2190)
                     /locus_tag="PLQ11_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24176.1"
                     /translation="MMTARRLDQFYTREDVARLCIDRVGRYAGRLATPLFVEPAAGAG
                     AFFNQLPASRVGMDLDPKCRGILKQDFLQWRPERSERPIVVVGNPPFGKNASLAVRFF
                     NHAAQFADIIALIVPLTFQKQSTWNKLDRGFMLVEECLLAEYSFVFDDCPYDVPTVFQ
                     IWKRGGMLRPLERLPTRHGDFSFVAAPQAEFAFQRVGARAGLVSEEGLRKSPQSHYFI
                     KPTQRAGSVRQILASIDWTPIKCRTAGNPSIGKAELIAAYSAALSE"
     gene            complement(2251..3858)
                     /locus_tag="PLQ11_04410"
     CDS             complement(2251..3858)
                     /locus_tag="PLQ11_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009492276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRK24177.1"
                     /translation="MAVLQSTFESTTAEALANAAQWQGLRAVLGERRAAAALGGNVKT
                     RERHTARGKLLPRDRVLNLLDPGSAFLEIGGLAAYCMYDDDIHAAGMICGIGRVEGRE
                     VMVVCNDATIKGGTYYPMTVKKHLRAQEIARENRLPCVYLVDSGGANLPHQTDVFPDR
                     EHFGRIFYNQATLSALGIPQIAVVMGSCTAGGAYVPAMSDETIIVRKQGTIFLGGPPL
                     VKAATGEVVSAEDLGGAEVHTRKSGVADHYAANDSHALALARRIVANLNSVKSVDIAL
                     SDPREPRFDAADLEALVPVDLKKQYDIREVIARIVDGSEFDEFKVNYGTTLVTGFARI
                     HGIPVGIIANNGILFSESALKGAHFIELCCQRRIPLLFLQNISGFMVGSQYEAGGIAK
                     DGAKLVTAVASARVPKITVLVGGSFGAGNYGMCGRAYSPRFLFTWPNSRISVMGGEQA
                     ASVLATVRRDNMEADGRLWSEDEEEAFKAPIRARYEEEGSPYHATARLWDDGIILPSE
                     TRRVLGLAFSACLNAPVEETKFGVFRM"
     gene            4105..4812
                     /locus_tag="PLQ11_04415"
     CDS             4105..4812
                     /locus_tag="PLQ11_04415"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRK24178.1"
                     /translation="MAGHRSVIGALVVVTAGLSALPAVAAEEMSAMARGGRLYDKHFQ
                     ENKTAKPTADHAAYPIKGGQYGKDTSWRCKECHGWDGLGVDGAYSKGGHKTGIKGVNA
                     YWGKDPAAVAAIIRNPTHGYTEAMLSAQDVSDLSLFVTKGMPDMRTYVDVATNKSKGN
                     QAKGEGYFNTMCAGCHGLDGKKVPNAPSLGSVADNGWEMMHKVLNGQPGESMPAMRAL
                     DPQITADLVAHLQTLPK"
     gene            4881..5459
                     /locus_tag="PLQ11_04420"
     CDS             4881..5459
                     /locus_tag="PLQ11_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008549330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HRK24179.1"
                     /translation="MTDIAEARGGLFKRLFSPTARWSVFSLLLVGGIGGVIFWGGFNT
                     FMEYTNRLEFCISCHSMSKPLEEYKASAHYSNPSGVRAICSDCHVPKDWTAKLIRKVQ
                     ATGELWHHFNGKLSTPEKFEAHRAEMATRVWASMKANDSRECRNCHSYETMAFHKQSA
                     RAREKMEPAVKSGETCIDCHKGVAHKLPPRDD"
     gene            complement(5470..6045)
                     /locus_tag="PLQ11_04425"
     CDS             complement(5470..6045)
                     /locus_tag="PLQ11_04425"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HRK24180.1"
                     /translation="MLKALADLVLKLSGWTVPPMQDVPEKCVVIGYPHTSNWDFVILQ
                     LVAFHFGIKLNWLGKKQLFPFPFAGLMRKLGGIAVDRSAPQGLVQQVAEQIIASREPV
                     RLVIPPEGTRSKVEYWKSGFYRIALAAGVPIMVAKVDFANKVVGFGTRVELTGDVRVD
                     MERIRALAAGNAGRFPELASPARLKDEENAG"
     gene            complement(6148..6444)
                     /locus_tag="PLQ11_04430"
     CDS             complement(6148..6444)
                     /locus_tag="PLQ11_04430"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRK24181.1"
                     /translation="MSGFRFWLVMAGLAAVTTPALAGNCIGTVQGLASSYDPARGSGF
                     LAVRSGPSTGAEQIGELFNGDRVDTGKSSGPWVYVIGESVEGWVHGRWLRIRCP"
     gene            complement(6518..7168)
                     /locus_tag="PLQ11_04435"
     CDS             complement(6518..7168)
                     /locus_tag="PLQ11_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRK24182.1"
                     /translation="MSLTLVIGNKAYSSWSLRPWLALKQMGIAFDEVLIPLDQPETKA
                     AIARHSPAGRVPVLIDGDLAVWESLAILEYVNETHANGRLWPQDRVARAHARVIANEM
                     HAGFSALRNALPMNVRRPIGALPMSVAVLADIARIETLWRDARQRYGQDGPFLFGAFS
                     AADAMYAPVVFRFHHYAVPVAEDTRAYMDRMRALPALREWVVAGTIEPWIIADDEK"
     gene            7302..8507
                     /locus_tag="PLQ11_04440"
     CDS             7302..8507
                     /locus_tag="PLQ11_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012452739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HRK24183.1"
                     /translation="MTSIVLIGAGPAGLIAAEHLAGLGHSVTVFDRMASPARKFLFAG
                     RGGLNLTHSEPHEAFLARYGAAAGRLAPLLAAFPAEALRSWAEGLGEPTFIGSSGRVF
                     PKSFKATPLLRAWLRRLDRLGVSFRFGMRWTGWDETGALIFETDAGRQKVHADAVLLA
                     LGGASWPRLGSDGGWVPLLAARGIAVTPLAASNVGVRIDWRAPFIERFHGAPLKGCAF
                     RLGDGTSRAEAVITRDGMEGGAVYALSAPLRAALAKGSAELAIDLKPDLSEAALSQRL
                     ARARTSETLSNILRKNASLPLIAIGLLREATGGPLPREATSLARRIKDVRLPVAGPGG
                     LARSISTAGGIAWEAVDEAQMLTALPGTFVAGEMLDVDAPTGGYLLQAAFSTGMAAAR
                     GMAAWLQRA"
     gene            complement(8810..11776)
                     /locus_tag="PLQ11_04445"
     CDS             complement(8810..11776)
                     /locus_tag="PLQ11_04445"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HRK24184.1"
                     /translation="MMDKTYDPATIEARVARAWDEARAFAAGAGARGDKTPFCIVIPP
                     PNVTGSLHMGHALNNTLQDILVRFERMRGKDVLWQPGTDHAGIATQMVVERKLAAEKK
                     DDRRTMGRERFLEEVWAWKAESGGTIVNQLKRLGASCDWSRERFTMDPGLSEAVIEFF
                     VGLHEAGLIYKDKRLVNWDPKLLTAISDLEVEQREVKGSFKWSRGETDKEGNPVSLNH
                     AALGKLLDREPGGHLYYFDYPVVDDTGKETGAVVTVATTRPETMLGDTAVAVHPTDER
                     YTHLVGKRVRLPLVGRLIPIVADEYSDPEKGTGAVKITPAHDFNDFEVGKRQGVPAIN
                     VMTPDGKMTFIRSNTFWRNTDFEAKLGVEDLVSELGRYDEDGEYIEFEIDDTQVDWLR
                     SNLEGVERFTARKAVIKEMDEAGRLQKIEPTVQQVPHGDRSGVPIEPFLTDQWYVNVK
                     PLADASMEAVKSGRTRMMPANREADFFRWMENIEPWCVSRQLWWGHQIPAWYGPDFPA
                     DPDDYYVARNEEEAQKKAQSRRASPSPRGRGEGKGEGRNGHVPLTRDPDVLDTWFSSA
                     LWPFSTLGWPEETPDLAKYYPNAVLITGFDILFFWVARMMMAGLFTQGEVPFRDVYLH
                     GLVRDEKGQKMSKSKGNVLDPLVVIDALGADALRFTLAALAAQGRDPKLGVKTAEGYR
                     NFCTKLWNASRFAEMNGCARIAGFDPGAVRITLNRWIVGETRKAAEAITLGITGYRFN
                     DAAAAAYRFVWNTFCDWYLELAKPVLQGPDGAEKDEARATTAWALDQIVKLLHPFMPF
                     ITEELWAAYSERAGEAPGLLCLQHWPELKGLEAPEAEAEIGWLVDLVTEIRSARAEMN
                     VPAASQIPLVLVAADDSVRARLARHDEVLKRLARLSDIGFTDAAPAESMQVLVRDTNA
                     ALPLAGIIDLNAERARLNRDIERIDQDVARIDAKLGNADFLARAPEEVVEEQRERRDE
                     ALARKQKLAEALARLG"
     gene            complement(11867..12742)
                     /locus_tag="PLQ11_04450"
     CDS             complement(11867..12742)
                     /locus_tag="PLQ11_04450"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019903886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HRK24185.1"
                     /translation="MTACAPSDPALSAAYQAAQATVRALDEDRYLATLFAPEAARPHL
                     FALYAFSAEISRVRSQVREAMPGEIRFQWWRDFLNGKPHGDASGHPLARALSATIGAC
                     ALPVKPLLDLIEARTFDLYDDPMPTWLDCEGYCGETSSALIQLACLCLTGGSDPGAAQ
                     AAGHAGVAYALTGLLRAFPWTSRRGQLFLPADVFAAHGVKPEAVRAGQDSPGLRAALG
                     EIRARAGDHLAKTRALIGTVSPAIAPAFLPVAMVDPYLKTMARADYDPFRSRVEVGKL
                     AKLWRFWRQARCAGI"
     gene            complement(12798..13178)
                     /locus_tag="PLQ11_04455"
     CDS             complement(12798..13178)
                     /locus_tag="PLQ11_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH938/NDUFAF3 family protein"
                     /protein_id="HRK24186.1"
                     /translation="MAEPGRGFVPGRHPIEGIGRGGFVFAGMSHIGSVLALPSGVHAW
                     AVSDPVEITPESLALAVAEAGDFEFLILGTGARQAFPTAAVRAVLAEAGLRCEAMATP
                     MATQTYNILLDEGRRVAAALIAVA"
     gene            complement(13178..14125)
                     /gene="secF"
                     /locus_tag="PLQ11_04460"
     CDS             complement(13178..14125)
                     /gene="secF"
                     /locus_tag="PLQ11_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HRK24187.1"
                     /translation="MKLLRIIPDGTKFPFMHWRRYSFPLSALLSIIAVVLFFTVNMNY
                     GIDFTGGTLIEMRAKSGQLDIAKVRSTAEHLGLGEPEVQAFGGNVPEVSMRFPLQPGG
                     DKGQQVVVEKARAAFINDFDFRRVEVVGPRVSGELVQAGTIGVVVSIIGVLIYLWFRF
                     EWQFALGAVIATLHDLVMTIGFYSLSQIEFNMTSIAAILTIVGYSLNDTVVVFDRIRE
                     LLRRYKKMPIKDLLDLAINQTLARTFLVSFTAMLALLALVMFGGTVISSFSWAMLVGV
                     VVGTYSSIFIAAPVLIYLGLKVGGDEPRSEAAAEARAAA"
     gene            complement(14250..>15546)
                     /gene="secD"
                     /locus_tag="PLQ11_04465"
     CDS             complement(14250..>15546)
                     /gene="secD"
                     /locus_tag="PLQ11_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003611020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HRK24188.1"
                     /translation="PAQPRGAAVRVVNPDDKAKVIAKLKDLSQPVDVARLGTAVRNLD
                     VVEQGDLITFTYSEAGINDRARRAVDQSLDVFRRRVDPEGTKEISIQRQGIDRVLIQV
                     PGLQDPTELREILGKVAKLEFRLVAEPGANPADVETLASDEGGPMQIERRVMVDGADL
                     TNAEPGFDQRTGQPVVNFRFNVSAARKFGQVTTDNVGKPFAIILDNKVISAPRILSPI
                     TGGQGQISGNFTVESANRLAILLRSGALPANFTIVEERTVGPGLGQDSIDAGKKAAYV
                     AAGLVFLFMLLTYGLFGIFANLALAIHILLIFAIMTKLGATLTLPGIAGIVLTIGTAV
                     DANVLIYERIREENHLGRSMIASLDAGFNRAMATIIDSNVTMFIAAAILYFLGSGPVR
                     GFAVTMIVGIITTIVTAVTMTRLMIALWYRWFKPTKIPF"
BASE COUNT         3021 a         5030 c         4758 g         2737 t
ORIGIN      
        1 gaggcggccc gttccggccg gccagccaga ggcaagcgcc cgttccgggg ccggccgcag
       61 cacaaccgga gcgccgggcg gggctgacct gtcgcacttg cgcaggatca ttgacggatg
      121 gccggccgca tcgacaatgc aatggtcaac acgagggagc ggaccatgac cctgcatcgc
      181 ttcatgatcg gttttggtgc tttctgcgca atggccctgc cggccgttgc tcagacctgg
      241 caggcggcgc cgccgaccgg cgccaaactc gggctcaagg ccatcggatg caagggagag
      301 ttctgccttg ccgtcgcctg cgtcggcggc aagccgcagc tcgtatcgct gtcgcccggc
      361 ggcggaccgt tcaatggcgc gaccaaggtc acggtcgggc cggccagcgc atcggtgacc
      421 tttgtggaag acccgaagat catggacgcg ttcaacatgc tcggaacgcg cgccccggtc
      481 atgccggagc tgatcgccga tatggcgacg gccaaggaca tcacgctggc cggaccgacg
      541 ttttccgaca aggtgacctc acggttcctg ctgaacggct acgccaaact ggcgccggcc
      601 atcgccaggg attgcgggat caagggcagc ggcagccagc ctgcgggaga gtgagccaag
      661 gtcagttgtg ttgacagaaa ggtcagccaa gtggcgagga aattcacgga ttgaaaaccc
      721 gccgcccgct cttgagcgtg atcggcagac cgaggtcacc gatttcctcc gcatggcagc
      781 ggtacgtggg ggcgggctga ccgctctggc caacaaaatc gggcatcttc tttgccagcg
      841 cgaccaaact gtccaacggc acactgcatt gcagccgacg ctggcccttg ctgtcgatct
      901 ttggattgag gcgaaccaat cccaaattgt gcaggacttt cgcatgaact tcccggaacc
      961 agtctcgcgc ttcctcttcc tcgccatgat cgaaggcggc gagtccgatg ccttcatgca
     1021 gttcagcgac cgcagccgcg ctgatatttc cgcgcaatcc tgcaaacatg cagccatgaa
     1081 ccagaatttc ctcgacccgg acgaatttct tgtgcacgcc atcctgtacg tagacaccga
     1141 tcagcatccg aaatggatga ttgatagacc agaacttacg tgcatccgag agagaaaccg
     1201 tattcgtgcc ggttgccttg atgctggttg gaatgcctaa aagcctgtca aatttttctt
     1261 caatatcgaa tccagatatt ggtgaacgct tgctatctgc tgccccacta aacaagccac
     1321 agcctttcag taagtcctca aatttcttgc cgtgtaattg gttggacatg taacaattcc
     1381 ggaaccacaa ggcatctact ctgaaagagc cgccgaataa gcggcaataa gctcagcctt
     1441 accgatgctt ggatttccag ctgtgcggca tttgattggc gtccaatcta tgctggccag
     1501 aatctgacgg accgagccag cgcgttgcgt aggcttgatg aaataatgcg attgcggtga
     1561 tttgcgcaac ccctcttccg agacgagccc ggcccgcgcg ccaaccctct gaaatgcaaa
     1621 ttcagcctgc ggcgcagcaa cgaaggaaaa atcaccgtgc ctggtcggta gacgctccag
     1681 cgggcgcagc attccccccc gcttccaaat ctgaaaaacc gtcgggacat cataggggca
     1741 atcatcaaaa acaaacgagt attccgcgag cagacattcc tcaaccagca taaacccgcg
     1801 gtccagtttg ttccatgtgg attgcttctg aaatgtcaac ggcacaatca acgcgatgat
     1861 gtccgcaaac tgtgccgcat ggttgaagaa tcgcacggcc aatgaggcat tcttgccaaa
     1921 tggcggattg ccgaccacaa cgatggggcg ctcacttcgc tccggtcgcc attgcagaaa
     1981 atcctgcttc aggatgcctc ggcacttagg gtcaagatcc ataccaaccc gcgaggcggg
     2041 caactggttg aaaaacgccc ctgcaccggc cgcaggctcg acaaacagcg gcgtcgcaag
     2101 acgccccgca tatcgcccaa cccgatcaat acataaacgt gcgacatcct cgcgtgtata
     2161 gaattgatcg agccgccgcg ccgtcatcat gctccagaat cacccttttc aacataaata
     2221 agaacgtatc atgaacatat ggattgtcaa ttacatcctg aacacgccga acttcgtctc
     2281 ttccaccggc gcgttgaggc aggcggaaaa cgccagcccc agcacgcggc gggtttcgga
     2341 aggcaggatg atgccatcgt cccacagccg cgccgtggcg tggtagggcg agccctcctc
     2401 ttcataccgg gcgcggatcg gcgccttgaa ggcttcctcc tcgtcctcgg accacaaccg
     2461 tccgtctgcc tccatgttgt cgcggcggac ggtggcgagc acgctggctg cctgctcgcc
     2521 gcccatcacc gagatgcggc tgttgggcca ggtgaacagg aaccgcggcg aataggcgcg
     2581 gccgcacatg ccgtagttgc cggcgccgaa cgagccgcca accagaaccg tgattttcgg
     2641 cacgcgcgcc gaggccacgg cggtgaccag cttggcgccg tccttggcga tgccgccggc
     2701 ctcatactgg ctgccgacca tgaagccgga gatgttctgc aggaacagca gcggaatgcg
     2761 gcgctggcag cacaattcga tgaaatgggc gcccttgagc gcgctttcgg agaacaggat
     2821 gccgttgttg gcgatgatgc cgaccggaat gccgtggatg cgggcaaagc cggtgaccag
     2881 cgtcgtgccg tagttgacct tgaactcgtc gaattccgag ccgtcgacga tgcgggcgat
     2941 cacctcgcgg atgtcgtatt gcttcttcag atcaaccggc accagcgcct caaggtcagc
     3001 ggcgtcgaac cgtggctcgc gcggatcgga gagggcaatg tccaccgact tgacgctgtt
     3061 gagattggcg acaatgcgcc gggccagagc cagcgcatgg ctgtcgttgg cggcgtaatg
     3121 atcggccaca ccggacttgc gcgtgtgcac ttcggcgccg ccgagatctt cggccgagac
     3181 gacctctccg gtcgccgcct tgaccagcgg cgggccgccg agaaagatcg tgccctgctt
     3241 gcgcacgatg atcgtctcgt cgctcatcgc cggaacatag gcgccgcccg ccgtgcagga
     3301 gcccatcacc accgcgatct gcggaatgcc gagcgccgac agcgttgcct gattgtagaa
     3361 gatgcggccg aaatgctcgc ggtcgggaaa aacatcggtc tgatgcggca gattggcgcc
     3421 gcccgaatcg acaaggtaga cgcagggcag gcgattctcg cgggcaatct cctgcgcgcg
     3481 cagatgcttc ttcaccgtca tcggataata ggtgccgccc ttgatggtcg catcgttgca
     3541 gaccaccatg acctcccgcc cctcgacccg gccgatgcca cagatcatgc cggcggcgtg
     3601 gatatcgtcg tcatacatgc aataggccgc caatccgccg atttccagaa aggccgagcc
     3661 cggatcaagc aggttgagca cacgatcgcg cggcagaagc ttgccccgcg ccgtgtgccg
     3721 ctcgcgcgtc ttgacgttcc cgcccagcgc cgcagcagcg cggcgctcgc caagcacggc
     3781 acgcaacccc tgccattggg cagcgttggc cagcgcctcg gcggtggtgg actcaaaggt
     3841 cgattgtagg acagccaaca gacactcccg tcatcaccgg gctgttatcc tgcccgcgca
     3901 actgtcccgt caagactgga gtcacaggaa cgtgcattct tgtcaaaata cataatttct
     3961 gccgctttgt catagttact tgcttttttg caaatacgct ttgcgcaaga acccttctca
     4021 ccgggcgatt gccgatgttg tctgtcttcg gagaatggta ggctgcggtt ggggtcaagc
     4081 aaacactcaa agggggaatt ggctatggca ggacatcgta gcgtcatcgg cgcgcttgtg
     4141 gtcgtcactg cggggctttc ggccttgccg gccgtcgctg cggaagagat gtccgcgatg
     4201 gcgcgcggcg ggcggcttta tgacaagcat ttccaggaga acaagaccgc caagccgacc
     4261 gccgatcatg cggcctatcc gatcaagggc ggccagtatg gcaaggatac ctcctggcgc
     4321 tgcaaggaat gccatggctg ggacgggctc ggggtcgacg gcgcctattc caagggcggc
     4381 cacaagaccg gcatcaaggg cgtcaacgcc tattggggca aggacccggc agcggtcgcg
     4441 gcaatcatcc gcaaccccac ccatggctat accgaagcca tgctctcggc ccaggatgtc
     4501 agcgatctgt cgctgttcgt caccaagggc atgccggaca tgcgcaccta tgtcgatgtc
     4561 gccaccaaca agtccaaggg caaccaggcc aagggcgaag gctatttcaa cacgatgtgc
     4621 gccggctgcc acgggctcga cggcaagaag gttcccaacg cgccctcgct cggctctgtc
     4681 gccgacaatg gctgggaaat gatgcacaag gtgctcaacg gccagccggg tgaatcgatg
     4741 ccggccatgc gggcactcga tccgcagatc accgccgatc tcgtcgccca tcttcagacc
     4801 ctgccgaagt aagccgggct gggcatgaat tgcgcgcgcc agttgctggc gcgcgcgtct
     4861 gtattgcgct gggggcgccg atgaccgaca ttgccgaggc ccggggcggg ctgttcaaac
     4921 gcctgttctc gccaaccgcc cgctggagcg tgttctcgct gctgcttgtc ggcggcatcg
     4981 gtggcgtgat cttctggggc ggcttcaaca ccttcatgga atacaccaac cggctggaat
     5041 tctgcatctc ctgccattcg atgtcgaagc cactcgagga atacaaggca tcggcgcatt
     5101 attcgaaccc ctcgggggtg cgcgccatct gttcggattg ccatgtgccg aaggactgga
     5161 ccgccaagct gatccgcaag gtgcaggcga ccggtgaact ctggcaccat ttcaacggca
     5221 aactgtcgac cccggagaaa ttcgaagccc atcgcgccga gatggcaacc cgcgtctggg
     5281 cgtcgatgaa ggccaatgac tcgcgcgaat gccgcaactg ccacagctat gaaacgatgg
     5341 cattccacaa gcaatcggcc cgcgcacgtg agaagatgga gccggcggtc aagtccggcg
     5401 aaacctgtat cgactgccac aaaggcgttg cgcacaagct gccgccgcgc gacgattgag
     5461 gcccccgcct cacccggcgt tttcctcgtc cttcaggcgg gccgggctgg caagctcagg
     5521 aaaccggccg gcattgccgg cggccagggc gcggatgcgt tccatatcga cccgcacatc
     5581 gccggtcagc tcgacacggg tgccgaatcc aacaaccttg ttggcgaaat cgaccttggc
     5641 caccatgatc ggcacgccgg cggccagagc gatacgataa aagccggatt tccagtattc
     5701 gaccttcgag cgcgtgccct cgggcgggat caccagccgg acgggctcac gcgaggcgat
     5761 gatctgctca gccacctgct ggacgagccc ctgcggcgcg gaacgatcca cggcgatgcc
     5821 ccccagcttg cgcatcagcc cggcgaaggg aaacggaaac aactgcttct tgcccagcca
     5881 gttgagcttg atgccgaaat ggaaggccac cagttgcagg ataacgaaat cccagttgga
     5941 cgtgtgcgga tagccgatga ccacgcactt ttccggcaca tcctgcattg gcgggacggt
     6001 ccagccggac aatttcagca ccagatcggc taaagccttg agcatatcct tccccatgcg
     6061 atgcggacgc ccctcagatg gggctgctga tcgtacatgg gtctattgaa ccgcaaattc
     6121 caggcagagc aagcctgcct ctccgcttta tggacagcgg atgcgcaacc agcgcccatg
     6181 cacccagcct tcgacggatt cgccgatcac atagacccag gggccgctgg acttgccggt
     6241 atcaacccgg tcgccgttga acaactcgcc gatctgctcg gcgccggtcg acgggccgga
     6301 gcgcacggcc agaaagccgg aaccacgtgc cggatcatag gaactcgcca gcccctgcac
     6361 cgttccgatg caattgcccg ccagcgccgg cgtcgtgacc gcagcaaggc ccgccatcac
     6421 cagccagaac cgaaaaccgc tcatgaaacg cctccttgtc agcggcccat tgtcgcgctc
     6481 tggcggccac agccaaggcc gccaatcgtc gcggcggtca cttctcgtcg tcggcgatga
     6541 tccagggttc gatggtgccc gccaccaccc attcgcgaag ggccggcagc gcacgcatcc
     6601 tgtccatata ggcacgcgta tcctcagcca ccggcacagc atagtggtga aagcggaaca
     6661 ccaccggcgc atacatcgca tcggccgcag aaaacgcccc gaacaggaac gggccgtcct
     6721 ggccgtagcg ctggcgcgca tcgcgccaca gcgtctcgat ccgggcgatg tcggccagaa
     6781 cggcgacgct catcggcagg gcgccgatcg ggcggcgcac attcatcggc aaggcattgc
     6841 gcaatgccga aaagccggca tgcatttcat tggcgatgac gcgggcatgg gcgcgggcga
     6901 cacgatcctg aggccagagg cggccgttcg catgggtctc gttgacatat tcgaggatcg
     6961 ccagcgattc ccagacagcg agatcgccgt cgatcagcac cggaacgcgc ccggcgggcg
     7021 aatggcgcgc gatcgcagct ttggtttccg gctggtcgag cggaatcagc acctcgtcaa
     7081 aggcgatccc catctgcttc agcgccagcc atggccgcag cgaccacgac gaataggcct
     7141 tgttgccgat caccagtgtc aggctcattg tcatcccctc acattgccgc gccatgatgc
     7201 cgtcaacacc ccgctttgcc aaacgccaat cgctgatgga tgccatcgcc ggcaatgatg
     7261 aaccgcttga cgcaatcacc gccagcaggc accccggagc aatgacttcc atcgtcctca
     7321 tcggcgccgg cccggctggc ctgatcgccg ccgagcacct cgccggcctc ggccattccg
     7381 tgacggtgtt cgaccgcatg gcctcgcctg cgcgcaaatt cctgtttgcc gggcgcggcg
     7441 ggctgaacct cacccattcc gagccgcatg aggcgtttct tgcccgctat ggagcggcag
     7501 cggggcggct cgcgccactg ctggcggcct tcccggccga agccttgcgc agctgggcgg
     7561 aaggactggg cgagccgacc ttcatcggct catcaggccg ggtgtttccc aagtcgttca
     7621 aggcaacgcc gctgctgcgc gcctggctgc gacggctgga ccggcttggc gtttccttcc
     7681 gtttcggcat gcgctggacc ggctgggacg agaccggcgc gctcatcttc gagacggatg
     7741 ccgggcgcca gaaagttcac gccgatgcgg tgctgctggc gctgggcggg gcgagctggc
     7801 cgcggcttgg gtcggatggc ggctgggtgc cgctgctcgc ggcgcgcggc atcgcggtga
     7861 caccgcttgc tgcctcgaat gtcggggtca ggatcgactg gcgcgctccg ttcatcgagc
     7921 ggttccacgg cgccccgctc aagggctgcg ccttccgcct cggcgatggc accagccgcg
     7981 ccgaagcggt catcacccga gacgggatgg aaggcggcgc ggtctatgcc ctgtcggcgc
     8041 ccttgcgcgc cgcgctggcc aagggcagcg ccgagcttgc gatcgacctc aagccggacc
     8101 tttcagaagc tgcgctgagc cagagactgg cccgcgcccg cacatcagaa accctttcca
     8161 acatattgag aaaaaacgca tcattgcctc tgatcgcgat cggcctgctg cgcgaggcga
     8221 cgggcggacc gctgccgcgc gaggcaacct cccttgcccg gcgcatcaag gatgtgcggc
     8281 tccccgtcgc cgggcctggc ggcctggccc gttccatctc cacggccggc ggcatcgcct
     8341 gggaggctgt ggacgaagcg cagatgctga ccgccctgcc cggcactttc gtcgccggcg
     8401 agatgctcga tgtcgatgcg ccgaccggcg gctacctgtt gcaggcggct ttctcgaccg
     8461 gcatggccgc cgcgcgcggc atggctgcgt ggctgcaacg ggcctagcta tctagagcac
     8521 tttcagtgaa ttcacattca ctgaaagtgc tctaatttat tgttttgtcg cattttcttc
     8581 acgcgaaccg gatcccattt cgcttgaaaa tgctctaatt tattgttttg tcgcattttc
     8641 ttcacgcgaa ccggatccca cttcgcttga aaatgctcta gtggcccttg ccaaggcaag
     8701 ggcgtccctc gcatcggctc ggcgtcgctt cgcagtcctg taagtggccc ttgccaaggc
     8761 aagggcgtcc ctcgcatcgg ctcggcgtcg cttcgctcct ataaggcagt tagcccaacc
     8821 gggccagcgc ctcggcgagc ttctgcttgc gggccagcgc ctcgtcgcgg cgctcacgct
     8881 gttcctcgac cacttcttcc ggcgcacggg cgaggaagtc ggcattgcca agcttggcgt
     8941 cgatgcgcgc cacatcctga tcgatgcgct cgatgtcgcg gttgagacgg gcgcgctcgg
     9001 cattgagatc gatgatgccg gcgagcggca gcgcggcatt ggtatcgcgc accagcacct
     9061 gcatcgattc tgccggcgcg gcatcggtaa agccgatgtc agacaggcgg gcgaggcgct
     9121 tgagcacctc gtcatgacgg gcaaggcggg cgcgcacgct gtcgtcggca gcgaccagaa
     9181 ccagcggaat ctggctggca gccgggacat tcatctcggc gcgagcggag cggatttcag
     9241 tcaccaggtc aacgagccag ccaatttcgg cttcggcctc cggcgcctca aggcccttga
     9301 gctcgggcca atgctggagg cacagcagcc ccggcgcttc gccggcacgc tccgaatagg
     9361 ccgcccagag ttcctcggtg atgaatggca tgaacgggtg cagcagcttg acgatctgat
     9421 cgagcgccca ggcggtcgtg gcgcgcgcct cgtccttctc ggcgccgtcc ggcccctgca
     9481 acaccggctt ggcgagctcc agataccagt cgcagaaggt attccagaca aagcgatagg
     9541 ccgccgccgc cgcatcgttg aagcgatagc cggtgatgcc aagggtaatc gcttcggcgg
     9601 ccttccgggt ttcgccgaca atccagcggt tcagcgtgat cctgaccgct cccggatcga
     9661 agccggcgat gcgtgcgcaa ccattcatct cggcaaagcg cgaggcgttc cacagcttgg
     9721 tgcagaagtt gcgatagccc tcggcggtct tgacgcccag cttggggtcg cgcccctgcg
     9781 cagcgagcgc ggcgagggta aagcgcagcg catcagcgcc gagcgcatcg atcaccacca
     9841 ggggatcgag cacattgccc ttggatttcg acatcttctg gcccttctcg tcgcggacga
     9901 gaccatgcag atagacgtcg cggaacggca cctcgccttg cgtgaacagg ccggccatca
     9961 tcatccgcgc cacccagaag aacaggatgt cgaaaccggt gatcagcacc gcattggggt
    10021 agtatttcgc cagatccggc gtctcttccg gccagccaag ggtcgagaac ggccacaaag
    10081 cggaagagaa ccaggtgtcg agcacatcgg ggtcgcgggt gagtggcaca tggccattcc
    10141 tcccctcacc cttaccctct ccccgcccgc ggggagaggg agatgcgcgg cgagactgtg
    10201 cctttttctg cgcttcttcc tcattgcggg cgacataata atcatcggga tcagcaggaa
    10261 aatccggccc ataccacgcc gggatctggt ggccccacca gagctggcgg gagacgcacc
    10321 acggctcgat attctccatc cagcggaaga aatccgcctc gcggttggcc ggcatcatcc
    10381 gcgtcctgcc ggacttcaca gcctccatgg aggcatccgc cagcggcttg acgttgacat
    10441 accactggtc ggtcaggaac ggctcgatcg gcacgccgga gcggtcgcca tgcggcactt
    10501 gctgaacggt gggttcgatc ttctggagcc gtccagcctc gtccatctcc ttgatcacgg
    10561 ccttgcgtgc agtgaaacgt tcgacacctt caagattgct acggagccag tcgacctgtg
    10621 tgtcatctat ttcaaattca atatactcac catcctcatc ataacgacca agctcagaaa
    10681 ctaaatcttc gacaccgagt tttgcctcaa aatcagtatt cctccaaaat gtgttgctgc
    10741 ggatgaacgt catttttccg tccggcgtca tcacgttgat cgccggaacg ccctgccgct
    10801 tgcccacctc gaagtcgttg aaatcatgcg ccggggtgat cttgaccgcg ccggtgccct
    10861 tttcggggtc ggaatactca tccgcgacga tggggatcag ccggccgacc agcggcagac
    10921 gcaccctctt gccgacaagg tgggtatagc gttcatcggt cggatgcacg gcgacggctg
    10981 tgtcgcccag catcgtctcg ggccgggtgg tagcgacagt gacgacggcg ccggtttcct
    11041 tgcccgtatc atcgaccaca ggatagtcga aataatagag atggccgccc ggctcccggt
    11101 cgagcagctt gccgagtgcg gcatggttga gggaaaccgg gttgccctcc ttgtcagtct
    11161 cgccgcgcga ccacttgaag ctgcccttca cttccctctg ctcgacttcg agatcggaga
    11221 tggcggtgag cagcttgggg tcccagttga ccagacgctt gtccttgtag atcagccccg
    11281 cctcgtgcag gccgacgaag aactcgatca ccgcctcgct gaggccgggg tccatggtga
    11341 aacgctcgcg gctccagtcg caggaggcgc cgaggcgctt caactggttg acgatggtgc
    11401 cgcccgattc cgccttccag gcccagactt cctcaaggaa gcgctcgcgc cccatggtcc
    11461 ggcggtcatc cttcttctcg gcagccagct tgcgctccac caccatctgc gtggcgatgc
    11521 cggcatgatc ggtgccgggc tgccacagca catccttgcc gcgcatccgt tcgaagcgga
    11581 ccagaatatc ctgcaacgta ttgttgagcg catggcccat gtgcagcgag ccggtaacgt
    11641 tcggcggcgg aatgacgatg cagaacggcg tcttgtcgcc gcgcgcgccg gcgccggcgg
    11701 caaacgccct ggcttcgtcc caggccctgg caacgcgggc ttcgattgtc gcgggatcgt
    11761 aggtcttgtc catcatggcc gtgcagtcgt gatcagggtc cggttcagtc gccctgttcc
    11821 ttccggcccg agccgccaat gtcaagaaaa aggctgcagg agatggtcag atcccggcgc
    11881 agcgggcctg ccgccagaac cgccagagct tggcgagctt gccaacctcc acccggctgc
    11941 ggaacggatc gtaatccgcc cgcgccattg tcttcaggta aggatcgacc atggccaccg
    12001 gcagaaaggc cggcgcgatt gccggggaca cggttccgat cagggcgcgc gttttcgcca
    12061 gatggtcccc ggcgcgggca cgaatctcgc caagggcggc ccgcaggccc ggcgaatcct
    12121 gcccggcgcg aacggcctcg ggtttgacgc catgggcggc aaagacgtcc gccggcagaa
    12181 acaactgccc gcgccgcgac gtccagggaa aggcgcgcaa cagcccggtc aaggcatagg
    12241 cgacaccggc atgaccggcg gcctgcgccg caccgggatc gctgccaccg gtcaggcaca
    12301 ggcaggcaag ctggatcagc gccgaggagg tctcgccgca ataaccctcg caatcgagcc
    12361 aggtcggcat cgggtcgtca tagaggtcga aggtgcgggc ttcgatcagg tccagcaggg
    12421 gcttgaccgg cagggcgcag gcgccaatcg ttgccgacag ggcgcgcgcc agcggatggc
    12481 cggacgcatc cccgtgcggt ttgccgttga gaaagtcccg ccaccactgg aagcggattt
    12541 cgcccggcat cgcctcgcgc acctgcgagc ggacgcggga gatctcggcc gaaaacgcat
    12601 aaagcgcaaa cagatgcggc cgggccgcct ccggcgcgaa cagggttgcc agatagcggt
    12661 cctcgtcgag cgcccgcacg gtcgcctgcg cggcctgata ggcggcagag agcgcgggat
    12721 cggacggggc gcaggctgtc atgggcacac tttcggcttg atcccgatat ggggcgcaac
    12781 tggccgaaat gaaagggtca tgccaccgcg atcagcgctg cggcaacgcg ccggccctca
    12841 tcgagcagaa tgttgtaggt ctgggttgcc atcggcgtcg ccatcgcctc gcagcgcaag
    12901 ccggcctcgg ccagcaccgc ccggaccgca gcggtgggaa acgcctgtcg ggcgccggtt
    12961 ccgaggatga gaaactcgaa atcgccggct tccgccactg ccagcgccag gctctccggc
    13021 gtaatctcca ccggatcgga cacggcccag gcatgcaccc cggacggcaa cgccaggacc
    13081 gaaccgatat ggctcatccc ggcgaaaaca aaaccgccgc ggccgatccc ctcgatcgga
    13141 tggcggccag gaacaaagcc cctgcccggc tcggccatca ggcggctgcc ctggcctcag
    13201 ctgccgcctc ggacctgggt tcgtcaccgc cgaccttgag gccgagatag atcagcaccg
    13261 gagcggcaat gaagatcgac gaataggtgc caaccacaac gccaaccagc atcgcccagg
    13321 agaaggacga aatcaccgtt ccgccgaaca tcaccagcgc cagcagtgcc agcatcgccg
    13381 tgaacgacac aaggaaggtg cgcgccagcg tctggttgat ggccagatcg agcaggtcct
    13441 tgatcggcat cttcttgtag cgtctgagca gctcgcggat acggtcaaac accaccacgg
    13501 tgtcattcag cgaatagccg acgatggtca gaatcgccgc gatcgaggtc atgttgaact
    13561 cgatctggct gagcgaataa aagccgatgg tcatcaccag gtcatgcagg gtggcaatca
    13621 ccgcccctag cgcaaactgc cattcaaatc ggaaccacag atagatcagc acgccgatga
    13681 tcgaaaccac cacgccgatc gtgccggcct gcaccagttc gcccgagaca cgcggaccga
    13741 ccacctcgac acggcggaaa tcaaaatcgt tgatgaaggc ggcgcgcgcc ttctcgacca
    13801 cgacctgctg gcccttgtcg ccgcccggct gaagcgggaa gcgcatcgag acttccggca
    13861 cattgccgcc gaatgcctgc acctccggct cgcccaggcc gagatgttcg gcggtggaac
    13921 ggaccttggc gatatcaagc tggccggatt tagcccgcat ctcgatcagc gtgccacccg
    13981 tgaagtcgat gccgtagttc atgttgacgg tgaagaacag caccaccgcg atgatcgaca
    14041 ggagcgctga cagcgggaag ctgtagcgcc gccagtgcat gaaagggaat ttggtcccgt
    14101 ccggaatgat gcgcaggagt ttcatgggcc ttgccttgga tcacgccgca ttttgataaa
    14161 accatcaaaa tgcggacaac gtgatcgatt tcaagtggtt atagcatgtc ttcatcgatg
    14221 aaccggtatc cacttcatcg cgacatgccc tagaacggga tcttggtcgg cttgaaccag
    14281 cgataccaca gcgcgatcat cagccgcgtc atcgtcactg ccgtgacgat cgtggtgatg
    14341 atgccgacga tcatggtgac ggcaaaaccg cgcaccgggc cggagccgag gaaataaagg
    14401 atcgccgctg cgatgaacat cgtcacgttc gagtcgatga tcgtcgccat ggcgcggttg
    14461 aagccggcat ccagcgacgc gatcatcgac cggccgagat ggttttcctc acggatgcgt
    14521 tcgtagatca gcacgttggc atcaaccgcc gtgccgatgg tcagcacgat gccggcaatg
    14581 ccgggcaacg tcagcgtcgc gccgagcttg gtcatgatcg caaagatcag caggatgtga
    14641 atcgccagcg ccagattggc aaagatgccg aacagcccgt aggtcagcag catgaacaag
    14701 aacaccaggc cggcggcgac gtaggccgcc ttcttgccgg cgtcgatcga atcctgcccg
    14761 agcccggggc cgacggtgcg ctcctcgacg atggtgaaat tggccggcag cgcaccggag
    14821 cgcagcagga tcgccagacg atttgcgctc tcgacggtga aattgccgga aatctgcccc
    14881 tgcccgccgg tgatcggcga cagaatgcgc ggcgccgaaa tgaccttgtt gtcaaggatg
    14941 atggcaaacg gcttgccgac gttgtcggtc gtcacctggc cgaacttgcg tgccgccgag
    15001 acgttgaagc ggaaattgac caccggctgg ccggtgcgct ggtcaaagcc cggctcggca
    15061 ttggtcagat ccgcgccatc gaccatcacc cgccgctcga tctgcatcgg cccgccctcg
    15121 tcggaggcaa gcgtctcgac atcggccgga ttggcgcccg gctcggcgac aaggcggaat
    15181 tccagcttgg caaccttgcc gagaatctcg cgcagctcgg tcgggtcctg caagcccgga
    15241 acctggatca gcacgcggtc gatcccctgc cgctggatcg agatttcctt ggtgccctcg
    15301 ggatcgacgc gccggcggaa cacatcaagc gactgatcga cggcacgccg ggcccgatcg
    15361 ttgatgccgg cctcgctgta ggtgaaggtg atcaggtcgc cctgctcgac cacatcgaga
    15421 ttgcggacag ccgtgccgag ccgggcaacg tcaaccggct gcgacaggtc cttcagcttg
    15481 gcgatcacct tggccttgtc gtccggattg acgacgcgca ccgccgcgcc gcgcggctgg
    15541 gccgga
//
LOCUS       DAOMXG010000041        15421 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_2127_length_15421_cov_9.040089, whole genome shotgun sequence.
ACCESSION   DAOMXG010000041 DAOMXG010000000
VERSION     DAOMXG010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 15421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15421
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2127_length_15421_cov_9.040089"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(393..1478)
                     /locus_tag="PLQ11_04470"
     CDS             complement(393..1478)
                     /locus_tag="PLQ11_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRK24189.1"
                     /translation="MHRRSFLKAGAAGVAASGLAAPALAQAAPEVRWRLTSSFPKSLD
                     TIFGTAETFARYVREASDGKFQIQTFSAGEIVPGLQALDSVSTGAVELAHTPTYFYVG
                     KDMTLGLGTGVPFGLNARLQHSWWHFGGGEAIVNEAMKPFGVYSIPCGNSGTQMGGWF
                     RKEIKTLDDLKGLKFRIGGLGGQVLAKLGVVPQQIAPGDVYPALERGTIDAAEFVGPY
                     DDEKLGLSKVAPYYYYPGWWEGGAMLHLCVNLDKWNSLTPQYQAIVRNACEAANNWML
                     AKYDAVNSQALGRLIQAGVQLRPFPMETIDAAYKAANEFYAEVSAKNPLFKKGYESMV
                     AFRKENYTWWQIGEYSYDSYIIRSSRG"
     gene            complement(1577..2539)
                     /locus_tag="PLQ11_04475"
     CDS             complement(1577..2539)
                     /locus_tag="PLQ11_04475"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRK24190.1"
                     /translation="MIRSLLASTLLIVTAMTAQAQDYPQKPVTLVVPFAPGGSSDVIS
                     RFVGQKLSEMWKQSVIVDNKAGGAGQIAMQAVARANPDGYTLILGHIGTLAVNPAMFD
                     KLSYNYKKEFLPVAMLAIVPSAIAVNPDLPIKSVKELLDYAKANPGKLNYGSAGNGSA
                     GHLSMEFFKDTVRMEAQHVPYKGTGPMLTDLLSGQTQLTYNGILPLVPHAKTGKLRII
                     GVGSPKRLPLMPDVPTIDEQGFPGFETSQWYGVLTPMGTPEAVVKKLNADINKVLADP
                     EIQKRLTDDGAVAGSGSPEDFGKYIDAEEKRWAPVVKKAGIKPD"
     gene            2672..3178
                     /locus_tag="PLQ11_04480"
     CDS             2672..3178
                     /locus_tag="PLQ11_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HRK24191.1"
                     /translation="MRGATIGLMATALMFALPAAAQEPDLIFKKSTVFKLLTPDDKLA
                     TYGIDDPVVEGVSCHFTVPEKGGIAGSLGLAEQVSDISLACRQIGPVRFKAKAEQGDV
                     VYRQSRSLFFKRMQVVRGCDEKRNVLVYLVYSDKLIDGSPKNSTSTVPIMPWGGTGEV
                     PKCGEFIK"
     gene            complement(3312..3626)
                     /locus_tag="PLQ11_04485"
     CDS             complement(3312..3626)
                     /locus_tag="PLQ11_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24192.1"
                     /translation="MLRLSALSLALVCSIASLVSAQSIPGEARQRDFVIQNDDGYGTS
                     DCLSRGSACGKIVADAWCEAKGFARAVAYRPAEAADITHSIRGPQRQSPLADSFVISC
                     SE"
     gene            3972..5405
                     /gene="cysS"
                     /locus_tag="PLQ11_04490"
     CDS             3972..5405
                     /gene="cysS"
                     /locus_tag="PLQ11_04490"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010915129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRK24193.1"
                     /translation="MAGGRNSDGFRGLRLTNTLTRDKAEFRPIDPANVRMYACGPTVY
                     DFAHIGNARPVIVFDTLFRLLRHLYGENHVTYVRNITDVDDKINARAFERWTKKRSLN
                     EEIRELTRVTAEQFHKDIAALGVLAPTHEPRATEFVCDSAKPLDMVRVIEGLIARGHA
                     YAAEGHVLFHVPSMPAYGRLSRRSMDDMIAGARVEVAPYKKDATDFVLWKPSNPETEP
                     GWESPWGFGRPGWHIECSAMSGALLGEVFDIHAGGLDLIFPHHENEIAQTCCFTGKEV
                     MANVWLHNGFLQVEGQKMSKSLGNFITINQLLQTTDFGGRTWPGEVLRFAMLKTHYRQ
                     PIDWTVKGLEEAEATLKRLYEKVAGIQPGQPSDEVIAALADDLNTPLAFAELHRIADT
                     TRLAATLKLLGFSCDLAAIGAGAAPVDAALAARVEPLIAARLAARAAKNFAESDRIRD
                     ELAAMGIALKDGKGPDGQPVTTWEVKR"
     gene            5602..6096
                     /locus_tag="PLQ11_04495"
     CDS             5602..6096
                     /locus_tag="PLQ11_04495"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRK24194.1"
                     /translation="MTARLRPFLPADAAALADLFRASIEILAEEDYSDAQREAWASAA
                     DDVDDFGKWLAGMLTLVVEDDSGLVGFGALEDNTMIALLYTRPDFARRGVATLLCDAL
                     ERLAAARGTSLLYTDASDTSQAFFANRGYTPFKRNSVMRAGEWLANTTMQKKLAPLRP
                     QGAA"
     gene            6096..7691
                     /gene="cimA"
                     /locus_tag="PLQ11_04500"
     CDS             6096..7691
                     /gene="cimA"
                     /locus_tag="PLQ11_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HRK24195.1"
                     /translation="MAKDRLYLFDTTLRDGAQTTGVDFSLADKRQIATMLDKLGVDYI
                     EGGYPGANPVDTAFFAERPPIRAKFTGFGMTKRAGLSASNDPGLNGLLAAKADAICYV
                     AKAWDYHVHVALGCTLKENLESIADSVKTAAERGFEPSVDCEHFFDGFKNNRDYALVC
                     ARTAYEAGARWVVLCDTNGGTLPHEVAAIVAEVAKVIPGDRLGIHAHDDTGQAVANSL
                     AAVLAGARQIQGTLNGLGERCGNANLVTLIGTLKLKPELASRFEVGVSDEALKGLTAM
                     SRAFDELMNRQPRRQAPYVGASAFATKAGIHASAVLKDPRTYEHVEPEAVGNRRLVLV
                     STQAGKSNVLSELERLGITVEKGDPRIARLLDEVKEREALGYSYEGADASFFLLAQKV
                     LGRVPDYFKVERFKVNVERRYNANGQLVTFSEAVVKVNIGGRTRISAAEGDGPVNALD
                     KALRKDLGRYQDHIKGLELSDFRVRIFDGGTEAVTRVLIESKDETGESWTTVGVSSNI
                     IDASFQALTDSIVYKLIKDGVKG"
     gene            7700..8614
                     /locus_tag="PLQ11_04505"
     CDS             7700..8614
                     /locus_tag="PLQ11_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018428885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRK24196.1"
                     /translation="MADQRTRLHPDGPELSRIVWGAWQVLMQPELGTPAGLAEMIVAC
                     LDHGITSFDHADVYGSFTVEARFGEALKLWGGARHSIEIISKCGNATLSPQRPETRVK
                     HYNTSAAHIAASVDRSLANFGTDYLDLLLIHRPDPFMDADDTAAGLEAVVRAGKVRAV
                     GVSNFTPQQVSLLQSRLSMPLVTNQIQFSLLHLAPLDDGTLDQAQERRMAPMLWSPLA
                     KGRLFREDSPVANRVREALGPMAARYGVEQGALALAWLMAHPARPVPVIGTSSVARIA
                     SLAKAEGLHLDRQDCFTLLEAARGAPAP"
     gene            complement(8615..8956)
                     /locus_tag="PLQ11_04510"
     CDS             complement(8615..8956)
                     /locus_tag="PLQ11_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24197.1"
                     /translation="MVRQAALITSLLLVSLQAPAFAQSRAKAAKPPAAVTITNARAGA
                     TMTDFGLTDAEGKVIARLAKPLASGKSASLKLGKGAKCVMTAIANFDDMTDSTSGDVD
                     VCKDKVVRFTD"
     gene            complement(9044..9205)
                     /locus_tag="PLQ11_04515"
     CDS             complement(9044..9205)
                     /locus_tag="PLQ11_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24198.1"
                     /translation="MVLKPVSFARYSDLVGLLLALLILLALAMRAQVTEPLPFGVRAS
                     APATIAHSA"
     gene            complement(9317..9904)
                     /locus_tag="PLQ11_04520"
     CDS             complement(9317..9904)
                     /locus_tag="PLQ11_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HRK24199.1"
                     /translation="MALIQNICVFCGSGVGRDPAFEQAADQFGTIMARAGIGLVYGGG
                     SIGLMGTVARAVLNGGGRVTGIIPGFLHKREIRFEGATEMIVTNDMHERKRLMFEKSD
                     AFVALPGGVGTLEELVEQMTWAQLGQHEKPILVADIGHFWKPLLVLLAHMREAGFIRE
                     SMEVHYLVAERVSEILPMLEKAAQNAAVRQVRASA"
     gene            9925..11901
                     /locus_tag="PLQ11_04525"
     CDS             9925..11901
                     /locus_tag="PLQ11_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HRK24200.1"
                     /translation="MVNASTALGVGVRVMPAYIAISALPALSLKARPVTASQPPKATL
                     ASTLASLWPYIWPQSRRDLQMRVALALVMILVSKGFTMLMPYTFKWATDAMVAAAGGK
                     VAGDAMTVLIGAPLLATVLYGVVRILMIVFTQIREGIFAPVSMHAARRTAILTFEHMH
                     RLSLRFHLERKTGGLTRILERGRSGVEDLARMLMMNGLPTIVEFLMVIGVFAYEFDWR
                     YVVVVIVMIIAYLWFTIAATNWRIKIRRQMNDSDNDANTKAVDSLLNYETVKYFGAEA
                     RETARYDVSMAKFESASVKTYSSLAFLNMGQAVIFTIALAVCMIMSAIDVQNGKNSVG
                     HFVMINALMIQLAQPLNFMGMLYREIKQNIVDIEQMFRILHQGAEVQDGPGATPLKVG
                     DGAIRFENVHFAYVPERPILKGVSFEVPAGRTVAIVGPSGAGKSTISRLMFRFYDVND
                     GRISIDGQDLRSVSQASLRAAIGMVPQDTVLFNDTILYNIGYGRAGASRADIEEAARL
                     AQIDGFIRQLPEGYDSQVGERGLKLSGGEKQRVAIARTILKGPPILILDEATSALDTF
                     TEREIQGALDHVSKGRTTLVIAHRLSTVVNADEILVLDQGQIVERGRHTDLLAQAGVY
                     AGMWNRQREAHEAEETLKRVKSAEGQSVRVQLGN"
     gene            12097..12318
                     /locus_tag="PLQ11_04530"
     CDS             12097..12318
                     /locus_tag="PLQ11_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24201.1"
                     /translation="MKIWMVFPIGLGLMLISAPAAMAQQKINPNSCQADISKMCSRPP
                     MTPATVKACLLHNKDKLAPDCRKIMNYGR"
     gene            complement(12387..12638)
                     /locus_tag="PLQ11_04535"
     CDS             complement(12387..12638)
                     /locus_tag="PLQ11_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24202.1"
                     /translation="MDVNAVFECIGFGASRAVASANFVAGTSSRVPARYILETARDPV
                     AYCIEALARHDPEAAARIRRPERVLILERLRREAGLVRH"
     gene            12955..13653
                     /locus_tag="PLQ11_04540"
     CDS             12955..13653
                     /locus_tag="PLQ11_04540"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HRK24203.1"
                     /translation="MSVVASVRKMIVPIHPEGYIFIAGAVILALIGGWIWSPLGWLFW
                     LAAGFCAYFFRDPPRVTPSRDGLVISPADGRVDMVGFFVPPPELGLGDKPLPRITIFL
                     SVFDVHINRAPVGGKIVKIAYTPGLFLNADLDKASEDNERNGMVIETASGRIGVVQIA
                     GLIARRIVCFVKEGETVGPGERYGLIRFGSRADIYLPDTARPMVGVGQRAIGGETILA
                     DLKSTETPRTFRVA"
     gene            13714..14523
                     /locus_tag="PLQ11_04545"
     CDS             13714..14523
                     /locus_tag="PLQ11_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylcholine/phosphatidylserine synthase"
                     /protein_id="HRK24204.1"
                     /translation="MSSAGPTPTQTEPVEPARRRGIPVRVLIPNLITLMVLAAGLTSV
                     RFAIEGRLEHSVMAIAVAALLDGLDGRIARLLKGASRFGAELDSLADFLAFGCAPALV
                     LYFWAMHEISTLGWLAAVALAMAAALRLARFNVSIEDTTRPEWTKAFFTGVPAPAGAM
                     LALLPINLYLSGLPRFPGHAAVVAVFTVFVAGLMVSRLPTLSGKGKGGAIPRDKVIPL
                     VALVLALGVLLISHPFPTVAALTIAYLATLPYSLHLYGRLRAATPPAEPPR"
     gene            14520..>15421
                     /locus_tag="PLQ11_04550"
     CDS             14520..>15421
                     /locus_tag="PLQ11_04550"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HRK24205.1"
                     /translation="MSGPAGKVASVAVAGSINLDIVMTAERLPGPGETVSGSGFRLAL
                     GGKGANQAVAARKLGADVTFVGRTGGDGFGGIVRRALADFGLDLAHVTTSDAAGTGIA
                     TIAIDAAGQNAITIVAGANADLSPADLDAADAALRRCGVLLLQCETPLATSLEAARRI
                     RSAGGTVILDPAPVPPQGIAAFAGLADIVTPNESEARLLTGIAVNGRDAALAAARVLV
                     GQGFSVAVVKCGGAGLAWSGRFGEGMAPAFAVPVVDTVAAGDVFNAALGVGLAESCPF
                     PAALSMAQAAAALAVTRPGAAGSAP"
BASE COUNT         2747 a         4890 c         4944 g         2840 t
ORIGIN      
        1 agccgccgcc gagccggccg cgactgcaac cgccaatgcc agcccggcgg ctcttgcgag
       61 ccgggccatc agcggcacgg ggaacagcga acgggtcacc ggcggtcctt tggcccaggc
      121 aagcctgaca aggctagcgt gaacggttaa caaactatga cgagacgtgg ttagagcatg
      181 ctgcgaaaaa gtgtgaacgg tttttcgctg aaagcatgct ctaaactacc agaatcgatc
      241 acattgtccg tgttttgatg attccaccaa aacacagcgt gatccagtga accattaatg
      301 cggctttgca aaggcagaaa cggtgccggg ccacacaaaa tggccgccgg catgggccgg
      361 cggcgctgga aagtcggcca aagccggtga actcacccgc gcgaggagcg gatgatgtag
      421 ctgtcgtagg aatattcgcc gatctgccac caggtgtaat tttccttgcg gaaggcgacc
      481 atcgattcgt agcccttctt gaacagcgga ttcttcgccg agacttccgc atagaactcg
      541 ttggcggcct tgtaggcggc gtcgatcgtc tccatcggga aggggcggag ctggacgccc
      601 gcctggatca gccggcccag cgcctgcgaa ttcaccgcgt catacttggc cagcatccag
      661 ttgttggccg cctcgcaggc attgcggacg atcgcctgat actgcggcgt caggctgttc
      721 catttgtcga gattgacgca cagatgcagc atcgccccgc cttcccacca gccggggtag
      781 tagtaatagg gcgcgacctt cgacaggccg agtttctcat cgtcataggg gccgacaaat
      841 tccgccgcgt cgatcgtgcc gcgttccagc gccggataga catcgccggg agcaatctgc
      901 tgcggcacga cgccgagctt ggccagcacc tgcccgccga ggccgccgat gcggaatttc
      961 agccccttga gatcatcgag cgtcttgatc tccttgcgga accagccgcc catctgggtg
     1021 ccggaattgc cgcaggggat cgaatagacg ccgaacggtt tcatcgcctc attgacgatg
     1081 gcctcgccac cgccgaaatg ccaccaggaa tgctgcagcc gggcatttag gccaaaggga
     1141 acgccggttc cgaggccgag cgtcatgtcc ttgccgacgt agaagtaggt cggggtatgg
     1201 gctagctcga ccgcgccggt cgagacggaa tcgagcgcct gcaggccggg cacgatctcg
     1261 cccgccgaga aggtctggat ctggaacttg ccgtcgctcg cctcgcggac atagcgggcg
     1321 aaggtctcgg ccgtgccgaa gatcgtgtcg agcgatttgg gaaagctcga cgtcaaccgc
     1381 cagcgcacct caggggctgc ctgggcaaga gccggggccg ccagcccgct ggcagcaacg
     1441 ccggctgctc cggccttcag aaacgatctg cgatgcatgg acttgaccct cctggtctca
     1501 ctcgatgcgg cactgtgcgg cggctacgga cgaacggcaa ggttagtcgg gccgccgcac
     1561 atcgggttga tccggatcaa tccggcttga tgcccgcctt cttgaccacc ggcgcccacc
     1621 gcttttcctc ggcatcgata tacttgccga aatcctccgg cgagccggac ccggcgacgg
     1681 ccccgtcatc ggtcagtcgc ttctggattt ccgggtcggc cagcaccttg ttgatgtcgg
     1741 cattgagctt tttcacgacc gcctcgggcg tgcccatcgg ggtcagtacg ccataccatt
     1801 gcgaggtttc gaaacccgga aagccctgct cgtcgatggt cggcacatcc ggcatcagcg
     1861 gcaggcgctt cgggctgccg acgccgatga tgcgcagctt tccggtcttg gcatgcggca
     1921 ccagcggcag gatgccgttg taggtcaatt gcgtctggcc cgagagcaga tcggtcagca
     1981 tcggcccggt tcccttgtag ggcacatgct gggcctccat cctgacggta tccttgaaga
     2041 attccatcga cagatggccg gccgagccat tgccggccga gccgtagttg agcttgcccg
     2101 ggttggcctt ggcgtaatcc agcagctctt tcaccgactt gatcggcagg tccggattga
     2161 cggcgatcgc cgacggcacg atggccagca tcgccaccgg caggaactcc ttcttgtaat
     2221 tgtaggagag cttgtcgaac atcgcaggat tgaccgccag cgtgccgatg tggccgagaa
     2281 tgagcgtgta gccatcggga ttggcgcggg ccacggcctg catggcgatc tgcccggcgc
     2341 cgcccgcctt gttgtcgaca atcaccgact gcttccacat ctcggaaagc ttctggccga
     2401 cgaagcgcga aatcacatcc gacgagccgc caggggcgaa ggggacgacc agggtaaccg
     2461 gcttttgcgg gtaatcctgc gcctgggcag tcatcgccgt gacgatgagc agcgtcgatg
     2521 cgagcagtga acggatcatt caagttacct cccgaattga cctttcctgt ctatcccgtc
     2581 caacaggtct tgcaaagtgc gaacccggca ttggaaaatg gctcggcaac agccatattc
     2641 accgcagatg catggtttcc ggagacattg aatgcgtggc gcaacgattg gcctgatggc
     2701 cacagccctg atgttcgccc tgcccgcagc ggcgcaggaa cctgacctca ttttcaagaa
     2761 atcgacggtt ttcaagctgc tgacgcccga cgacaagctt gccacctacg gcatcgacga
     2821 ccctgtggtc gagggggttt cctgtcattt caccgtgccg gagaagggcg gcattgccgg
     2881 ctcgctgggg ctggctgagc aggtatccga catttcgctt gcctgccggc agatcggacc
     2941 ggtcaggttc aaggccaagg ccgagcaggg cgatgtcgtc tatcgccagt cgcgttcgct
     3001 gttcttcaag cgcatgcagg tcgtgcgcgg ctgcgacgaa aagcgcaacg tgctggtcta
     3061 tctcgtctat tccgacaagc tgatcgacgg cagcccgaag aattctacct cgaccgtgcc
     3121 gatcatgccc tggggcggca ccggcgaggt gcccaaatgc ggcgagttca tcaaatagcg
     3181 cgagtgactg actggcacgg tcatcgggcc gctggtcagg cgcgaaggcg acgccgagca
     3241 gggccaaaag gcccggttgc gaggaaacga cctgagggtg accgacaggt caaacccgaa
     3301 ggccgaaggt cttattccga gcaggaaatc acgaaggaat cggccagcgg gctctgccgt
     3361 tgcggtccgc ggatcgaatg cgtgatatcg gccgcctcgg ccggccggta ggccacggcg
     3421 cgggcaaaac cctttgcctc gcaccaggca tcggcgacga tcttgccgca ggccgagcct
     3481 ctgctgaggc agtcggatgt gccatagccg tcgtcgttct ggatgacgaa atcccgctgg
     3541 cgcgcctcgc cgggaattga ctgggccgaa accagggagg caatggagca aacaagcgca
     3601 agcgacaggg cagacagacg aagcataacg acagttccga tggatccccg attcgaggac
     3661 agtgcagagc aaatgtgaac aaatggctaa aacagggcag cgcagtgatt ttgcgcacgc
     3721 agccgtgttt tgctcattgt ggttttttcg cgctgttgct gcgatgcatc attccgcgcc
     3781 gcgatgcaac cgcttgacgt gatgccgtaa acaggcaatg gaaggccatg acgcgcgcaa
     3841 tccttaactc tgtcgggatt atttgcggct agctttcaag cgctggccga cgccgtctca
     3901 ttcccaccga tcacggaccg aacgagacac caccggctgg cgcgggggtg tgatgcgctg
     3961 gagaacgtga catggcaggc ggacgcaatt cggacggttt caggggcctc aggctcacca
     4021 ataccctgac gcgcgacaag gcggaattcc gcccgatcga tcctgccaac gtgcggatgt
     4081 atgcctgcgg cccgacggtc tatgattttg cccatatcgg caacgcgcgc ccggtgatcg
     4141 tgttcgacac gctgttccgc ctgctgcgcc atctctacgg cgaaaatcac gtcacctatg
     4201 tccgcaacat cacggacgtc gacgacaaga tcaacgcccg cgctttcgag cgctggacga
     4261 agaagcgctc gctcaacgag gaaatccgcg aactgacgcg ggtcaccgcc gagcagttcc
     4321 acaaggacat cgccgcgctc ggcgtgctgg cgccgacgca tgagccgcgt gccaccgaat
     4381 ttgtctgcga cagcgccaag ccgctcgaca tggtgcgggt gatcgagggg ctgattgccc
     4441 gcggccatgc ctatgcggca gagggccatg tgctgttcca tgtcccctcg atgccggcct
     4501 acgggcggct gtcgcgccgt tcgatggacg acatgatcgc cggcgcgcgg gtcgaggtgg
     4561 cgccctacaa gaaggatgca accgacttcg tgctgtggaa gccgtccaac ccggagacgg
     4621 aaccgggctg ggaaagcccc tggggtttcg gccgtccggg ctggcatatc gaatgctcgg
     4681 cgatgtcggg cgcattgctc ggcgaggtgt tcgacattca cgccggcggg ctcgatctca
     4741 tcttcccgca ccacgagaac gaaattgccc agacctgctg cttcaccggc aaggaggtga
     4801 tggccaatgt ctggctgcac aacggatttc tgcaggtgga agggcagaag atgtccaaaa
     4861 gcctcggcaa tttcatcacc atcaaccagc ttctgcaaac gacggatttc ggcggccgca
     4921 cctggccggg cgaggtcttg cgctttgcca tgctgaaaac ccactatcgc cagccgattg
     4981 actggacggt gaaggggctg gaggaggcgg aggcgaccct gaagcggctt tacgagaagg
     5041 tcgcaggcat ccagcccggc cagccctcgg atgaggtgat cgccgcgctg gccgacgatc
     5101 tcaatacgcc tttggccttt gccgaactgc acaggatcgc cgacaccacc cggcttgcgg
     5161 ccacgctgaa gctgctcgga ttttcctgtg atctggcggc catcggcgcc ggggccgcgc
     5221 ctgtcgatgc agcgctcgcc gccagggtcg agccgctgat tgccgcccgt ctcgccgccc
     5281 gtgccgccaa aaacttcgcg gagagcgacc gcatccgcga tgaactcgcc gcgatgggca
     5341 tcgctctcaa ggacggcaag ggaccggatg gtcagcccgt gaccacctgg gaggtgaagc
     5401 ggtgaccacc ccctcccgcg cctccgcatg cagtgaagtc tacgcagact gcgtatactt
     5461 gtctgcggcg ctcggaccac cctccccgca agggggaggg attgttgcta acggttccgc
     5521 atctctccct cctcttgatg gggagggtgg cggcgcagcc gccggaaggg gtgctctgcg
     5581 agccatgaaa ggtcgcatcc catgactgcc cgcctgcgcc ccttccttcc cgccgatgcc
     5641 gccgcgctcg ccgatctgtt ccgcgcctcg atcgaaattc tcgccgagga ggattattcc
     5701 gatgcccagc gcgaggcctg ggcctcggcc gccgacgatg ttgatgactt cggcaaatgg
     5761 ctcgccggca tgctgacgct ggtggtcgag gatgacagcg gtctggtcgg cttcggtgcg
     5821 ctggaggata acacgatgat cgcgctgctt tacacgcggc cggatttcgc ccggcgcggc
     5881 gtcgccacgc tgctctgcga tgcgctggaa aggctcgcgg cggcgcgcgg cacgtccctg
     5941 ctctacaccg atgccagcga cacctcgcag gcgttcttcg ccaatcgcgg ctatacgccg
     6001 ttcaagcgca acagcgtgat gcgcgccggc gaatggctcg ccaatacgac aatgcagaag
     6061 aaacttgccc ctctccgccc gcagggagcc gcctgatggc caaggatcgc ctctacctgt
     6121 tcgacaccac cctgcgcgat ggcgcccaga ccaccggtgt cgatttttcc ctggccgaca
     6181 agcggcagat tgcgacgatg ctggacaagc tcggcgtcga ttacatcgag ggcggctatc
     6241 ccggcgccaa cccggtcgat accgcctttt tcgccgaacg cccgccgatc agagcgaagt
     6301 tcaccggctt tggaatgacc aagcgggcag ggctgtccgc ctccaatgat cccggcctca
     6361 acggcctgct ggcggcgaag gcggatgcga tctgctacgt cgccaaggcc tgggactatc
     6421 atgtccatgt cgcgctcggc tgcacgctca aggaaaacct cgaaagcatc gccgacagcg
     6481 tcaagaccgc cgccgaacgg ggttttgaac cctcggtcga ttgcgagcat ttcttcgacg
     6541 ggttcaagaa caaccgcgac tatgcgctcg tctgcgcccg cacagcctat gaggccggcg
     6601 cgcgctgggt ggttctgtgc gataccaacg gcggcacgct gccgcatgaa gtcgccgcga
     6661 tcgttgccga ggtggcaaag gtgatccccg gtgaccggct gggtatccac gcccatgacg
     6721 ataccggcca ggcagtggcc aattcgctgg ccgccgttct ggccggcgcc cgccagatcc
     6781 agggcacgct caacggtctc ggcgaacgct gcggcaatgc caatctggtc acgctgatcg
     6841 gcactctgaa actgaagccg gagctggcca gccgcttcga ggtcggcgtc agtgacgagg
     6901 ctctgaaggg gctgacagcg atgtcgcgtg ccttcgacga actcatgaat cgccagccgc
     6961 gccggcaggc accctatgtc ggcgcctcgg cctttgccac caaggccggc attcacgcct
     7021 ccgccgtgct gaaggacccg cgcacctatg agcatgtcga gccggaggcg gtgggtaacc
     7081 gccgtctcgt gctggtctcg acccaggccg gcaagtcgaa tgtgctgtcg gaactggaac
     7141 ggctaggcat caccgtcgag aagggcgatc cgcgcatcgc ccgcctgctc gacgaggtga
     7201 aggagcgcga ggcgcttggc tattcctatg aaggggcgga tgcctctttc ttcttgctgg
     7261 cgcagaaggt gctgggccgc gtgccggact atttcaaggt tgagcgcttc aaggtcaatg
     7321 tcgagcgccg ctacaacgcc aacggccagc tggtgacctt ctccgaagcg gtggtgaagg
     7381 tgaacatcgg cggccgcacc cgcatttcgg cggcggaggg cgatggcccg gtcaacgcgc
     7441 tcgacaaggc gctgcgcaag gatctcggcc gctatcagga tcacatcaag gggctggaac
     7501 tctccgattt ccgcgtccgc atcttcgatg gcggcaccga ggcggtgacc cgcgtgctga
     7561 tcgaatccaa ggacgagacc ggcgaaagct ggacgacggt gggcgtcagt tccaatatca
     7621 tcgacgcttc gttccaggca ttgacggatt cgatcgtcta caagctgatc aaggatggcg
     7681 tgaaagggtg aggatgatca tggcggacca gcgcacgcgc ctgcacccgg acggtcctga
     7741 actctcgcgc atcgtctggg gcgcgtggca ggtgctgatg cagccggagc ttggcacgcc
     7801 ggcgggcctt gccgaaatga ttgtcgcctg cctcgatcac ggcatcacca gcttcgatca
     7861 tgccgatgtc tacggcagct tcacggtgga ggcgcgcttc ggcgaagcgc tgaagctctg
     7921 gggtggagcg cgccacagta tcgaaatcat cagcaaatgc ggcaacgcca cgctgtcgcc
     7981 gcaacgaccg gagacgcgcg tcaagcacta caacacgagc gccgcccata tcgccgcctc
     8041 cgtcgaccgc tcgcttgcca atttcggcac cgactatctc gacctgctgc tgatccaccg
     8101 gcctgatccg ttcatggatg ccgatgatac cgctgctggt ctggaagccg tggtccgtgc
     8161 cggaaaggtg cgcgcggtgg gcgtgtcaaa cttcacaccg cagcaggttt ctctgctgca
     8221 gtcgcgcctt tcgatgccgt tggtcaccaa ccagatccag ttttcgttgc tgcatcttgc
     8281 cccgctcgac gatggaacgc tcgatcaggc gcaggaacgg cgcatggcgc cgatgctttg
     8341 gtcgccgctg gccaagggac gtctgttccg cgaggatagc ccggtggcga accgtgtgcg
     8401 cgaggcgctc ggccccatgg cggcgcgata tggcgtggag caaggcgcgc tggcgcttgc
     8461 ctggctcatg gcgcatccgg cccgcccagt gccggtaatc ggcacctcat cagtggcgcg
     8521 catcgccagc ctggcgaagg ctgagggcct ccacctcgac cggcaggact gcttcacatt
     8581 gctcgaagcg gcgcgtggcg ccccggcacc ctgatcaatc ggtgaagcgg acgaccttgt
     8641 ctttgcagac atcgacatcg ccggaggtgc tgtccgtcat atcgtcaaaa ttggcgatgg
     8701 cggtcatcac gcatttggcg cccttgccga gcttcagcga tgccgatttg ccggaggcga
     8761 gcggcttggc aagccgggca atcaccttgc cctcggcatc ggtcaggccg aaatcggtca
     8821 tggtggcgcc ggcgcgggca ttggtgatgg tgaccgccgc cgggggcttg gcggccttgg
     8881 cgcggctctg cgcgaaagca ggcgcctgaa gtgaaaccag cagaagggac gtgatcaatg
     8941 ccgcttgacg aaccatgttg gaagccctga ctccgaggac ggcgaaaatg ccgtcgacca
     9001 tgctaggaca gcatgccggc ccgggcaagg accggtttgc cggtcaggcg ctgtgcgcga
     9061 tcgtcgcagg tgcggaggcg cggacgccga aaggcagcgg ttcggtgacc tgcgcgcgca
     9121 tcgccagcgc cagcaggatc agcagcgcca gcaacaggcc gacaagatcg gaatagcggg
     9181 cgaaggatac gggtttcagc accatttcgg cctcccgtgg ggagtttcga tcaggcgacg
     9241 ctagccgagc atggttaagc ggtgcttgcc gccgcccctc gtgtgatcaa catcggtaaa
     9301 tcggaggtta acagggtcag gcgctggcgc gcacctgccg cacagcggca ttctgggcgg
     9361 ccttttcaag catcggcagg atctcgctga cccgttcggc gacgagataa tgcacttcca
     9421 tgctctcgcg gatgaagccc gcctcgcgca tatgcgccag aagaaccagc agcggcttcc
     9481 agaaatggcc aatatcggcg accaggatcg gcttttcgtg ctggccaagc tgtgcccagg
     9541 tcatctgctc gacgagttcc tccagcgtgc cgaccccgcc gggcagggcg acgaaagcgt
     9601 ccgatttctc gaacatcagc cgcttgcgct cgtgcatgtc gttggtgacg atcatctcgg
     9661 tcgccccctc gaagcggatt tcccgcttgt gcaggaagcc ggggatgatg ccggtcaccc
     9721 ggccgccgcc gttgagcacg gcacgcgcca cggtgcccat cagcccgatc gagccgccgc
     9781 cataaacgag gccgatgccg gcccgcgcca tgattgtgcc gaactggtcg gccgcctgtt
     9841 cgaaggccgg atcgcgccca acgcccgaac cgcagaaaac gcagatgttc tgaatcagtg
     9901 ccatgcccct aggagagcgc gaaaatggtt aacgcttcaa ccgcgcttgg ggttggcgta
     9961 cgggtgatgc cggcctatat cgcgatcagc gcgctgcctg cgctgtcgtt gaaagcccgt
    10021 cccgtgactg cttcccagcc gcccaaggcg acgctcgcct ccactcttgc ttcattgtgg
    10081 ccctatatct ggccgcaatc gcggcgtgac ctgcagatgc gcgtggcgct cgcgctggtg
    10141 atgatcctcg tctccaaggg cttcaccatg ctgatgccct acaccttcaa gtgggcaacg
    10201 gatgcgatgg ttgcggcggc gggcggcaag gtcgcgggcg atgcgatgac ggtgctgatc
    10261 ggcgcgccgc tgctggcgac ggtgctttat ggcgtcgtgc gcatcctgat gatcgtgttc
    10321 acccagatcc gcgaggggat tttcgccccc gtttcgatgc atgcggcgcg gcgcacggcc
    10381 atcctgacct ttgaacacat gcatcggctg tcgttgcgct ttcatcttga gcgcaagacc
    10441 ggcgggctga cccgcatcct cgaacgcggc cgctccggag tcgaggatct ggcccgcatg
    10501 ctgatgatga acggcctgcc gacgatcgtc gagttcctta tggtcatcgg cgttttcgcc
    10561 tacgaattcg actggcgcta tgtcgtcgtg gtcatcgtga tgatcatcgc ctatctgtgg
    10621 ttcaccatcg cggcgaccaa ctggcgcatc aagatccggc ggcagatgaa cgacagcgac
    10681 aatgacgcca acaccaaggc cgtcgattcg ctgctgaact acgagacggt gaaatatttc
    10741 ggcgccgaag cccgcgagac ggcgcgctac gatgtctcga tggcgaaatt cgagagcgcc
    10801 tcggtcaaga cctattcctc gctggccttc ctcaacatgg gccaggcggt gatcttcacc
    10861 attgcgctcg ctgtctgcat gatcatgtcg gccatcgacg tgcagaacgg caagaacagc
    10921 gtcggccatt tcgtcatgat caacgcgctg atgatccagc tggcccaacc gctgaatttc
    10981 atgggcatgc tctaccgcga gatcaagcag aacatcgtcg atatcgagca gatgttccgc
    11041 atcctgcatc agggggccga agtgcaggat gggcccggcg cgacgccgct gaaggtgggc
    11101 gatggcgcga tccgcttcga gaatgtgcat tttgcctatg tgccggagcg gcccattctc
    11161 aagggcgttt catttgaagt gccggccggc cgcacggtcg ccatcgtcgg cccgtcgggc
    11221 gccggcaagt cgaccatctc ccggctgatg ttccgctttt acgacgtcaa cgacggccgc
    11281 atcagcatcg acggtcagga tctgcgcagc gtttcgcaag cctcgttgcg tgcggccatc
    11341 ggcatggttc cgcaggatac cgtgctgttc aacgacacga tcctctacaa catcggctat
    11401 ggccgcgccg gcgcctcgcg ggccgacatc gaggaggccg cgcgtcttgc ccagatcgac
    11461 ggcttcatcc ggcaattgcc ggagggttac gattcgcagg tcggtgagcg cggcctgaaa
    11521 ctgtccggcg gcgaaaagca gcgcgtcgcc attgcccgca ccattctcaa ggggccgccg
    11581 atcctcattc tcgacgaggc gacctcggcg ctggatacct tcaccgagcg ggaaatccag
    11641 ggcgcgctcg accatgtcag caagggcagg accacgctgg tgattgccca ccgcctgtcg
    11701 acggtcgtca atgccgacga gattctcgtg ctcgatcagg gccagatcgt cgagcgcggc
    11761 cggcacaccg acctgctggc tcaggccggt gtctatgccg gcatgtggaa ccgccagcgc
    11821 gaggcgcacg aggcggagga aacgctcaag cgcgtcaaga gtgccgaggg ccagagcgtt
    11881 cgggtccagc ttggcaattg acggccggcc gggcgccacc agagcatttt caagcgaagt
    11941 cgagcccggt tcgcgtgatg aaaatgcgac aagaccgaaa ggtgggtatc gctccggcgt
    12001 ttcatccttc gcggcgaagg tttcttaagc gtttggcgtt agttgttgca gcctgagccc
    12061 gcattgtcgg cggagcagca cgacacgagt atgcccatga aaatctggat ggtgtttccg
    12121 atcggacttg gcctgatgct gatcagcgct ccggcggcca tggcccagca gaagatcaac
    12181 cccaactcct gccaggctga tatttccaaa atgtgctcac ggccgccgat gacgccggca
    12241 acggtcaagg cctgcctgct gcacaacaag gacaagctgg ctcccgactg ccgcaagatc
    12301 atgaactacg ggcggtaatc gccagggcca ataaaaaacg cgcccacccg ggcgcgcttg
    12361 cgcatctgcc tcgccgcttg cggcggtcag tggcgaacca gccctgcctc gcgccgcagc
    12421 cgctcgagga tcagaacgcg ttcgggccgc cggatccgcg ctgccgcttc cggatcgtgc
    12481 cgggccagcg cctcgatgca ataggccacc ggatcccgcg ccgtctccag aatgtaacgc
    12541 gccggaacgc gggaagacgt gcccgcaacg aaattggcgc ttgccaccgc gcgcgaggcg
    12601 ccaaaaccga tgcactcgaa aacagcgttg acgtccatag ccttcctcct ttccggtcct
    12661 tatcagcgaa ccatgcgaca gcagtttgac accaagtcta cgcctaaagt ctagcaaaaa
    12721 atggcgaaca gacacagaaa cacaaccagg gcgggtaaat tgctgcattt taccgcattt
    12781 gtggcgggtt gaggtccttt gccgggcgga agtccggccc tccttttcat cctccctgcc
    12841 aggacctgat tcgccggcac ggcatcgctg ccggcggcgc gatccggctt cgaccttcta
    12901 tttgtgcgtt aaataggcta gaggagccgc ctgtcgttca ggaaagggcc ccccatgtcc
    12961 gtggtcgcat ctgtccgcaa gatgatcgtc cccatccatc cggaggggta tattttcatt
    13021 gcgggcgccg tgatcctcgc tctgatcggt ggctggatct ggtcgccgct cggctggctg
    13081 ttctggctgg cggcgggttt ttgcgcctat tttttccgtg atccgccgag ggttacgccc
    13141 agccgcgacg gtctggtgat ctcgccggcc gatgggcggg tggatatggt cggcttcttc
    13201 gtgccgccgc cggaactcgg gctgggcgac aagccgctgc cgcgcatcac gattttcctg
    13261 tcggtcttcg atgttcatat caatcgcgcg ccggttggcg gcaagatcgt caagatcgcc
    13321 tatacgccgg ggctgttcct caatgccgat ctcgacaagg ccagcgagga caacgagcgc
    13381 aacggcatgg tgatcgagac ggccagcggc cgcatcggcg tggtgcagat cgccgggctg
    13441 atcgcgcggc gcatcgtctg cttcgtcaag gaaggcgaga cggtgggccc cggcgagcgc
    13501 tatggcctca tccgcttcgg ctcgcgggct gacatctacc tgcccgacac ggcacggccg
    13561 atggtcggcg tcggccagcg ggcgatcggc ggcgaaacca ttctcgctga tctcaagagc
    13621 accgagacgc cgcgcacctt ccgcgtcgcg tgacgccagc gggcggacgt gcttaaactg
    13681 cacgtccagc cgcgccgcat cgagtgagcc gccatgtcat cagccgggcc gacacccacc
    13741 cagacggaac ctgtggagcc tgcccgccgc cgcggcattc cggtgcgcgt tctcatcccc
    13801 aatctgatca cgttgatggt gcttgccgcg gggctgacct cggtgcggtt cgccatcgag
    13861 ggacggctgg agcattcggt gatggcgatt gccgttgctg ccctcctgga cgggctggat
    13921 ggccgcattg cacggctgct caagggggct tcgcgcttcg gcgcggaact cgattccctg
    13981 gctgattttc tggccttcgg ctgtgcgcct gcgctggttc tgtatttctg ggcgatgcat
    14041 gagatttcga ccctcggctg gctggcggcg gtggcgctgg cgatggcggc ggcgttgcgt
    14101 cttgcccgtt tcaacgtctc catcgaggac accacccgtc cggaatggac caaggcgttc
    14161 ttcaccggtg ttccggcgcc ggccggcgcg atgctggcgc tgctgccgat caatctctac
    14221 ctgtcaggat tgccgcggtt tcccggccat gcggcggttg tggcggtgtt cacggtgttc
    14281 gtggcggggc tgatggtcag ccgcctgccg acgctgtcgg gcaagggcaa gggcggagcg
    14341 atcccgcgcg acaaggtcat tccgctggtc gcgctggtgc ttgccctggg ggtgctgttg
    14401 atcagccacc cgttccccac ggttgcggca ttgaccatcg cctatctggc gacgctgccc
    14461 tacagcctgc atctttatgg caggctgcgc gccgcgacac cgccggcgga accgcctcgg
    14521 tgagcggacc ggcgggcaag gtggccagtg tagcggtcgc cggttccatc aatctcgaca
    14581 tcgtgatgac ggccgagcgc ctgcctggcc ccggcgagac cgtttcagga agcgggttcc
    14641 gtctcgccct cggcggcaag ggcgccaatc aggcggtggc ggcgaggaag ctcggcgctg
    14701 atgtcacctt tgtcgggcgc accggaggcg acggcttcgg cgggatcgtg cgcagggcgc
    14761 tggccgattt cggactcgat cttgcccatg taaccacctc agacgcggcc gggacgggaa
    14821 tcgccaccat cgccatcgac gcggcaggcc agaacgcgat caccattgtc gccggcgcca
    14881 atgccgactt gtccccggcc gatctcgatg ctgccgacgc ggcgctgcgt cgctgcgggg
    14941 ttctcctgct gcaatgtgaa acgccgctgg cgacctcgct cgaagcggcc cgccgcattc
    15001 gatccgccgg cggaaccgtc attctcgacc ctgcgccggt gccgccgcag ggaatcgcgg
    15061 cctttgccgg tctggccgat atcgtcacgc cgaacgagag cgaagcgaga cttctgaccg
    15121 gaatcgccgt caacggtcgc gatgcggccc tggcagcggc ccgcgttctg gtggggcagg
    15181 ggttttcggt cgccgtggtc aaatgcggcg gggcagggtt ggcctggtcc ggacgattcg
    15241 gcgagggcat ggcgccggcc tttgccgtgc cggttgtcga tacggtggcg gcgggcgatg
    15301 tgttcaacgc cgccctgggg gtcggcttgg cagaatcatg tccgtttccg gccgcgcttt
    15361 ccatggcgca ggccgctgcg gcgctggctg tgacccgccc cggcgcggcg ggctctgcgc
    15421 c
//
LOCUS       DAOMXG010000042        15284 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_2152_length_15284_cov_10.364042, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000042 DAOMXG010000000
VERSION     DAOMXG010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 15284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15284
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2152_length_15284_cov_10.364042"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="PLQ11_04555"
     CDS             <1..554
                     /locus_tag="PLQ11_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRK24206.1"
                     /translation="CRLQGEPVAAVPRPRHTLVSQKMGIHEWSYDNVISVQARLKVPM
                     LDNAVALKDIRTEVELGYDAGLALAEAERCLNCDVQTIFEKGLCIECDACVDICPMDC
                     ITFTADGEDKDLRSRLSAPAKNLTQDLYISDPVKTGRVMVKDEDVCLHCGLCAERCPT
                     GAWDMQKFIVQLPRAGASCRSRA"
     gene            536..2371
                     /locus_tag="PLQ11_04560"
     CDS             536..2371
                     /locus_tag="PLQ11_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HRK24207.1"
                     /translation="MSIQSVNDFVVRFANINGSGSASANQMFARAVIRIGVPVAARNI
                     FPSNIQGLPTWYEVRVTGEGWQGARGGADLIVAMNPQTFDKDVAAVEPGGYVFYDSSR
                     PVPASKFRADVTVIGMPLTEICNAQYSDPRQRQLFKNIICVGALAALLDMDIAVVDKL
                     IAEQFAAKEKLIAANQKALHYGYDWAKKNLSCPIGLTIRKSTAVGDKIFIEGNSAAAL
                     GAVYGGATVLAWYPLTPSTSLAEAFTSHCKRLRHDPESGANRYAIVQAEDELSSIGMV
                     VGAAWNGARAFTATSGPGISLMQEFLGLAYYAEVPAVVFNVQRGGPSTGMPTRTQQSD
                     LMACAYASHGDTKHVLLIPRDAHEAFEFGALAFDLADRLQTPVFVMLDLDIGMNHALV
                     SPLKWDPNRKLDRGKVMTFEDLDSGKLFGRYLDVDGDGIPYRTYPGTHPSRGSFFTRG
                     SSHDSMARYTEEGGPVAENVVRLLKKFDTAKHYVPAPVIERAARRTRLGIIHFGSTGP
                     AMDEAVALLARKGIDLDVMRLRGFPFAAGVAEFIAEHDQVFVVEQNRDAQMRSMLMIE
                     EGIDPAKLVSVRYFEGPPITARYIAETIGKLASQAPVVAKEKALS"
     gene            2368..3423
                     /locus_tag="PLQ11_04565"
     CDS             2368..3423
                     /locus_tag="PLQ11_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HRK24208.1"
                     /translation="MTFLPKPKFHHPAIARNELGLTHRDYEGSISTLCAGCGHDSISS
                     AIMHACFELNLPPHRIAKLSGIGCSSKTPTYFLGPAHGFNSVHGRMPSVLTGANLANR
                     ELIYLGVSGDGDSASIGFGQFAHSIRRGVNMTYIVANNGVYGLTKGQFSATADKGARQ
                     KKGTINTDSPIDLVRMALQLGATFVGRSFSGDKTQLVPLIKAALEHKGPAFIDCLSPC
                     VAFNNHDGSTKSFDYVRAHNEALNSLDFVSERAPITTEYAPGEMQVVRLHDGSSIRLR
                     KIGDGYDVHDRHAAMTYLSQREDSGEIVTGLIYMHPEPEEMHEHLGTVATPLNRLSDQ
                     ELVPSQAALDKINAALR"
     gene            3673..4746
                     /locus_tag="PLQ11_04570"
     CDS             3673..4746
                     /locus_tag="PLQ11_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HRK24209.1"
                     /translation="MHLTTAIEALGENGVSALGGLGIGIVFGFWAQKSRFCLRAAAVE
                     FARGSTGVKVSVWLLVFATALVGTQALIAAGQIDVSETRQLAAQGSISGAALGGLMFG
                     FGMVLARGCASRLLVLSANGNLRALLSGLVFAVAAQASFRGLLAPAREQLTGLWLVDG
                     GPSRDIIAVFGGGTTMKVIFALVWLAAAIVFCWRNRIPALIIAGGIGTGLAVAAGWWF
                     TYALAQSAFSPVPVKSLTFSGPSADVLMFVLSGTLANVNFDIALVPGVFIGSFLAAAF
                     AGELKIEGFTDGLSMRRYIFGATLMGFGAMLAGGCAVGAGVSGASVFALTAWIVLVGM
                     WLGAALADLAFDRPRHAPAVQRP"
     gene            complement(4765..5157)
                     /locus_tag="PLQ11_04575"
     CDS             complement(4765..5157)
                     /locus_tag="PLQ11_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012752916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRK24210.1"
                     /translation="MRFNRRTLFHACLVVPLLPGYAAAAGFTKFTQAGFQQAQAAGRS
                     ILVVINAPWCPTCKVQDPIIAKLVGEAKYKNVLVLEVDFDSEKDILRAMKASKQSTLI
                     GFKGTKETGRSVGDTSPLGIEDLIEGTL"
     gene            complement(5225..6496)
                     /locus_tag="PLQ11_04580"
     CDS             complement(5225..6496)
                     /locus_tag="PLQ11_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRK24211.1"
                     /translation="MTLDRRQALQGVATLAGILYAPAVIGQAKPRVVVIGGGPGGATA
                     AKFIARDSQDAINVTLVEPSDKFVTCFHSNLYVGGYKTFQEITHSYDDLTKKYAINHA
                     RKTAAFIDRAAKVVHLTDSATVSYDRLVIAPGIDLKYDSVPGWGQEFEEIMPHAWKAG
                     PQTQILRKRLDEVKDGGVIVMIAPPNPYRCPPGPYERVSMMAHLLKSTGRGKCKIIVL
                     DPKESFSKQGLFQEGWERHYPGMVEWIGPKVHDGIKSVDPKTNTVVTGFETYKDVALV
                     NVIPAQMAGKIAREAQLTNQSGFCPIDPANMKSASDPNIFIVGDACIPGDMPKSAFAA
                     NSQAKVAANIIRSELTGSRAFPARYANTCWSLIETDDAVKVGGSYEPKDGKIFATETY
                     ISKTGEDAALRKQTQAENMGWYAGIVSDMFG"
     gene            complement(6540..7076)
                     /locus_tag="PLQ11_04585"
     CDS             complement(6540..7076)
                     /locus_tag="PLQ11_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HRK24212.1"
                     /translation="MTDTGRDTGPAIDRRHLIGALAAAGTMMGAGAEAAPATLALADI
                     KKEADAACLYHCDFGDPARFGQMLVNIGNHFSVYGNNPFDVQLAVVAHGAGIKFFLES
                     LEGTPWAEETTVPKIFERVEALAKSGLKVYLCAITFERQKIARDQARKAGFVQFVPSG
                     VATVAALQGKGFAYLKVG"
     gene            complement(7073..7381)
                     /locus_tag="PLQ11_04590"
     CDS             complement(7073..7381)
                     /locus_tag="PLQ11_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002710969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24213.1"
                     /translation="MTLLLVTAAHAQTAARKGDRELGEYLSNMCTACHQVSGRQVGGI
                     PAIVGWPEDQFVAVIQSYKDGTRDNETMRTLSHRLSQEDIDGLASYFGSLKPIAEAKK
                     "
     gene            complement(7417..8073)
                     /locus_tag="PLQ11_04595"
     CDS             complement(7417..8073)
                     /locus_tag="PLQ11_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRK24214.1"
                     /translation="MLSFSAQGQAPAKTGSPAATPAPAKKVGLGREAHPDEIKAWDID
                     VRPDGLGLPPGKGSVKQGEELYQAQCASCHGEFGESAGRWPVLAGGRGSLKNEGPEKT
                     VGSFWPFASTTFDYIRRAMPYGNARSLSDDEIYAITAYVLSLNDVVKDDFVLSKENFT
                     GVKLPNGEGFFDDDREKTERHFWAEPCMKACKPDPAKVTGRARVIDVTPDAKDGPKVD
                     "
     gene            complement(8114..9394)
                     /gene="soxC"
                     /locus_tag="PLQ11_04600"
     CDS             complement(8114..9394)
                     /gene="soxC"
                     /locus_tag="PLQ11_04600"
                     /EC_number="1.8.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008195948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite dehydrogenase"
                     /protein_id="HRK24215.1"
                     /translation="MSDMKTAGPLSRRGLLRGAGLAGASVAAGAFAGPAGAAEPEKVI
                     TEIQDWNRYLGPGVEAAPYGRPSKHEAHVVRRNVPWLTADTASSINFTPIHALDGIVT
                     PNGVCFERHHSGVAEVDPAKHRLMINGLVDKPLVFTLEDLKRFPGRVNRLYFLECAAN
                     GGMEWRGAQLNGCQFTHGMIHNVWYTGIPLKALLDEAGVKTNGKWVMPEGADAAAMTR
                     SIPIEKAMNDCLVAWAMNGEALRPEQGYPLRIVVPGWEGNMWVKWLRRIEVGDQPWHH
                     REETSKYTDLLADGRSRRFTWIMDAKSVVTNPSPQAPLKHKGPNVLTGLAWSGRGTIQ
                     RVDVTTDGGRNWTTARIDGPVLPLSLTRFYLDFDWDGREMMIQSRAMDSTGYVQPTKD
                     ELRKVRGVNSIYHNNGIQTWLVRSNGETENVEIS"
     gene            complement(9432..11129)
                     /gene="soxB"
                     /locus_tag="PLQ11_04605"
     CDS             complement(9432..11129)
                     /gene="soxB"
                     /locus_tag="PLQ11_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfohydrolase SoxB"
                     /protein_id="HRK24216.1"
                     /translation="MISRRHFLQATAAACALLPATGVGSLGRVAAQQKLGQSDILKFE
                     PLGTVTILHVTDIHAQLMPVYFREPSVNLGVGADRGVPPHLTDQAFREHFKIATGSAD
                     AHALTADDFVSLAKTYGRMGGMDRVATLIKAVRSERGDDKVLLLDGGDTWQGSWSALK
                     TDAADMVEIMEALKVDAMTSHWEFTLGEKRVKEIAEASSIAFLAQNIRSKEWQEEVFK
                     PRKMFEKGGVKIAVIGQAMPRTAIANPNWMFPDWEFGLREDELQKQVDDARGEGAAIV
                     VLLSHNGVDVDKKLASRVKGIDVVLAAHTHDAMPGVLKVGETAIVASGSHGKFVSRLD
                     IEVKDRKVAGLRYKLMPVFSDAIQSDAAMTGLIDKWRKPYQADLSRIVGQTDSLLYRR
                     GNFNGTFDDLICQAMLAERDAEISLSPGFRWGASLLPGDPITFEAIANATAMTYPNCY
                     RNEMTGEQIKLMLEDVADNIFHPDPYFQGGGDMVRVGGVGYSIDISKPINQRISDLTH
                     LKSGQPIDAARKYVVAGWASVAQNVAGPPIWEVVDRYVSRVKTVRLEPNTSVKVTGA"
     gene            complement(11183..11512)
                     /gene="soxZ"
                     /locus_tag="PLQ11_04610"
     CDS             complement(11183..11512)
                     /gene="soxZ"
                     /locus_tag="PLQ11_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HRK24217.1"
                     /translation="MATNSVPRVRVPAQAKAGDMIEIKTLISHEMESGQRKDSAGKTI
                     PRQIINKFTAVFNGKTVFEADWHPAISANPYQSFFFKAKESGEFKFTWKDDNGSDYTA
                     TAKLTVA"
     gene            complement(11529..11993)
                     /gene="soxY"
                     /locus_tag="PLQ11_04615"
     CDS             complement(11529..11993)
                     /gene="soxY"
                     /locus_tag="PLQ11_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HRK24218.1"
                     /translation="MASAHRLQSRRDLMLSAAVAGFATMLAPRLALADVKAVEDEIKK
                     LYGDKKIDSGKIKLDVPQIAENGLVVPITVEVESPMTDADFVKAVHVFADGNPLPGIA
                     SFRFSPECGKANASARMRLAQTQNIVCVAEMSNGALFSAKAEVKVTIGGCGG"
     gene            12181..13020
                     /gene="soxA"
                     /locus_tag="PLQ11_04620"
     CDS             12181..13020
                     /gene="soxA"
                     /locus_tag="PLQ11_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016669 - oxidoreductase activity, acting
                     on a sulfur group of donors, cytochrome as acceptor
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019417 - sulfur oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxA"
                     /protein_id="HRK24219.1"
                     /translation="MKRTRGKWAAFGLAVAAVALAAGMAPGMAQQDDAEKEIERYRAM
                     INDPMANPGFLFVDRGEALWSQKRGAKNVSLETCDLGEGAGKLEGAYAKLPRYFKDAD
                     KVMDLEQRLLWCMKTVQDLDTADVLKRRFGSPSNTSDLQDLVAFIANKSSGMKYQPQL
                     DHPKEKEMLAVGEALFYRRGGSQDFSCSTCHGESGKRIRLQGLPDFSKPGKDARETMG
                     SWPTYRVSQSQLRTMQHRLWDCYRQMRHPAPEFGSDGITALTVYMVKMGEGGEITVPS
                     IKR"
     gene            13033..13641
                     /gene="soxX"
                     /locus_tag="PLQ11_04625"
     CDS             13033..13641
                     /gene="soxX"
                     /locus_tag="PLQ11_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HRK24220.1"
                     /translation="MKKTILAALLCGLAGASLAQQAKVDPAAVEAAVKGMWAKAGPEW
                     ASSITPDETMKVCSETRNAPSAAEFEKIVAREKATVVFPADGNVMGDWKKGEIVASRG
                     TGGQFTDTPDTYRGGNCYACHQLAAKEVSYGTLGPSLLGYGKARNFSQEEAKVAYAKI
                     YNPQGVLACSQMPRFGHWKFLSEEQIKDAVAFLFHPDSPVNK"
     gene            13760..14269
                     /locus_tag="PLQ11_04630"
     CDS             13760..14269
                     /locus_tag="PLQ11_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRK24221.1"
                     /translation="MKKMRQPETRAFSSEVESGSREENAIKQEIGAFSVKLNSPISAL
                     VGPAMRKAIFGAALAAWVSVLNPPAGAAELVMVERDDCSWCKRWHAEVGESYMKAEEG
                     RQAPLRRWRLEQGQPKLAMKEPVRFTPTFILVEDGREIGRITGYLENGMFWGLLGDLL
                     KKAQSQQAR"
     gene            14360..14653
                     /locus_tag="PLQ11_04635"
     CDS             14360..14653
                     /locus_tag="PLQ11_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRK24222.1"
                     /translation="MMRNARDASDLLKALSHESRLLLLCILAEKERSVGELETILSLR
                     QPTVSQQLARLRYDNIVTTRRDGKTIYYSIANDDLRKIVRVIYDIFCVSRAAR"
     gene            14677..>15284
                     /locus_tag="PLQ11_04640"
     CDS             14677..>15284
                     /locus_tag="PLQ11_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HRK24223.1"
                     /translation="MDSLFGSPVPAGFLIGVIAGFFVRRARLCSFGAIESALIGGDWR
                     RMKVFGMALGVALVLTQTMALTGLIDPGHSTYVNARIPLLATAFGSLTFGLGMALVGT
                     CAFGSLVRLGSGDLRALVTLMVFGALSYATLRGVLSPMRLSLFEAVTISLPGAIAGTL
                     EAFAAAMGGALARVGLIGLLALGLVGAALLDPRLWRAPKLMTA"
BASE COUNT         2768 a         4696 c         4769 g         3051 t
ORIGIN      
        1 gctgccgcct ccagggcgag ccggtggccg ccgttccgcg gccgcgccat acgctggtca
       61 gccagaaaat gggaatccac gaatggagtt atgacaatgt catctcggtg caggcgcggc
      121 tgaaagtgcc gatgctcgac aacgcggtgg cgctcaagga tatccgcacc gaggttgaac
      181 tcggctatga tgcgggcctt gcgctggccg aggccgagcg ctgcctcaac tgcgatgtgc
      241 agaccatctt tgaaaagggc ctgtgcatcg agtgcgatgc ctgtgtcgat atctgcccga
      301 tggattgcat cacattcacg gcagatggtg aggacaagga tctgcgcagc cgcctttcgg
      361 cgccggccaa aaacctgacg caggaccttt acatctccga cccggtcaag acgggccgcg
      421 tcatggtcaa ggatgaggat gtgtgcctgc attgcggcct gtgcgccgaa cgctgcccga
      481 caggagcctg ggacatgcag aagtttatcg ttcaactgcc gcgcgcgggg gcatcatgtc
      541 gatccagagc gtgaatgatt tcgtcgtccg gttcgccaat atcaacggct ccggctcggc
      601 ctccgccaac cagatgtttg cccgcgcggt gatccgcata ggcgtgccgg ttgcagcgcg
      661 caatatcttt ccctccaaca tccagggatt gccgacttgg tatgaggttc gggttaccgg
      721 agagggatgg cagggagcgc gcggcggcgc cgatctgatc gtggccatga acccgcagac
      781 cttcgacaag gatgtcgcag ccgtcgagcc cggcggctac gtgttctacg attcctcgcg
      841 cccggtgccg gcctcgaaat tccgtgccga tgtcacggtc atcggcatgc cgctgacaga
      901 aatctgcaat gcccagtatt ccgacccgcg ccagcgccag ttgttcaaga acatcatttg
      961 cgttggcgcg cttgcggcac ttctggacat ggatattgcg gtcgtcgaca agctgattgc
     1021 cgagcagttt gccgccaagg aaaagctgat cgccgccaac cagaaggcgc tgcattatgg
     1081 ctatgactgg gccaagaaaa acctgtcctg cccgatcggc ctgacgatca ggaagtcgac
     1141 ggcggtcggc gacaagatct tcatcgaagg caattcggca gccgcgctcg gcgcggtcta
     1201 tggtggagcg acggttctcg cctggtatcc gctgacgccg tcaacgtcgc tggccgaggc
     1261 tttcacaagc cattgcaaac gcctgcggca tgatcctgaa tccggtgcca atcgctacgc
     1321 gatcgtccag gccgaggacg agctttcctc gatcggcatg gttgttggcg cggcctggaa
     1381 cggcgcgcgt gcctttacgg ccacctccgg ccccggcatt tcgctgatgc aggaattcct
     1441 gggtctggcc tattatgcgg aagtgccggc cgtggtgttc aacgtgcagc gcggcggccc
     1501 gtcgaccggc atgccgactc gcactcagca atcggatctg atggcctgcg cctatgcctc
     1561 gcatggcgat accaagcatg tgctgctgat cccgcgcgat gctcatgagg ctttcgaatt
     1621 cggcgcgctc gccttcgatc tcgccgaccg gctgcagacg ccggtgttcg tcatgctcga
     1681 tctggatatc ggcatgaatc atgcgctggt gtcgccgctc aaatgggacc caaaccgcaa
     1741 gctcgaccgt ggcaaggtga tgaccttcga ggatctcgac agcggcaagc tgttcggccg
     1801 ttacctcgat gtcgatggcg acggaattcc ctaccgcacc tatccgggca cgcacccctc
     1861 gcgcggttcc ttcttcacgc gtggctcttc gcacgattcg atggcgcgct acaccgagga
     1921 aggcgggcct gtcgccgaga atgtcgtccg cctgctcaag aagttcgata ccgccaagca
     1981 ctatgtgccg gccccggtca tcgaacgtgc cgccaggcgg acgcggctcg gaatcatcca
     2041 tttcggctcc accgggcccg caatggatga ggcggtggcg ctgctggcca ggaagggcat
     2101 cgatctggat gtgatgcgcc tgcgcggctt cccgttcgcc gccggggttg ctgagttcat
     2161 cgccgaacac gatcaggttt tcgtcgtcga gcagaatcgc gatgcccaga tgcgcagcat
     2221 gctgatgatt gaggagggga tagaccccgc caagctggtt tcggtgcgct atttcgaagg
     2281 cccgccgatt actgcgcggt acatcgccga gaccatcggc aagctcgcct cccaggctcc
     2341 ggtcgtcgcc aaggaaaagg ccctgtcatg acctttctgc ccaagccgaa attccatcac
     2401 ccggccattg cccgcaacga gcttggcctg acccatcgcg actatgaggg ctcgatctcg
     2461 accttgtgcg ccggctgcgg ccacgattcg atttcctcgg cgatcatgca cgcctgtttc
     2521 gagctgaatc tgccgccaca ccggatcgcc aaactctcgg ggatcggctg ttcctccaag
     2581 acgccgacct atttccttgg tccggcgcat ggcttcaatt cggtgcatgg gcgcatgccc
     2641 tcggtgctga ccggcgccaa tcttgccaac cgtgagctga tctatctcgg cgtttcgggc
     2701 gatggcgatt cggcctcgat cggtttcggc cagtttgccc attcgatccg tcgcggcgtc
     2761 aacatgacct atatcgtcgc caacaatggc gtctacggat tgaccaaggg ccagttctcg
     2821 gcaaccgcag acaagggcgc caggcagaag aagggcacga tcaacaccga ttcaccgatc
     2881 gatctggtgc ggatggcgtt gcagctcggc gccaccttcg tggggcgctc cttctcgggt
     2941 gacaagaccc agctggtgcc gctgatcaag gcggcgctgg agcataaggg gccggccttc
     3001 atcgattgcc tgtccccctg cgtggccttc aacaatcatg acgggtcgac caagagcttc
     3061 gattacgtgc gcgcccacaa cgaggcgctc aacagcctcg acttcgtgtc cgagcgcgcg
     3121 ccgattacga ccgaatatgc gcccggcgaa atgcaggtcg tccgcctgca tgatgggtcc
     3181 tcgatccgtc tgcgcaagat cggcgatggt tacgacgtgc atgaccggca cgctgccatg
     3241 acctatctgt cgcagcgcga ggacagcggc gagattgtca ccgggctgat ctacatgcat
     3301 cccgaacccg aagagatgca cgaacatctt ggcacggtcg caacgccgct caatcgcctg
     3361 tccgatcagg aattggtgcc cagccaggcg gcgctcgaca agatcaacgc cgccttgcgc
     3421 tgacccatcg gcaaacgtca cgccggctgg cgaagcgggg cgcttggagc attttcaagc
     3481 gaaggggagc ccggttcgcg tcaagaagat acgaaaaaac aaaaagatag agtacttccc
     3541 gaccggattg aaccgaacga ttcaatccgg tcggaaactc tggcatttga cgaaccgcaa
     3601 cgacactttc atgttcttga atatgaagag cggtggcgta tccagactct gtgcgcctat
     3661 gcggttgggg ccatgcatct aacgacagcg atcgaagcat tgggagagaa tggtgtctcg
     3721 gccctaggcg ggttggggat tggcattgtc tttggtttct gggcgcagaa gagtcggttc
     3781 tgcctgcgcg cggcggcggt ggaatttgcc cggggcagca ccggggtcaa ggtttcggtc
     3841 tggcttctgg tgttcgcgac cgcgctggtc ggaacccagg cattgattgc cgcggggcag
     3901 atcgacgtca gcgaaacacg gcaattggcg gcgcagggca gcatatccgg cgcggctctg
     3961 ggtggattga tgttcggctt cggcatggtg ctggcgcgtg gctgtgccag tcgtcttctg
     4021 gtgctttccg ccaacggcaa tctgcgcgcg ctgctgtccg gtctggtttt cgcggttgcg
     4081 gcgcaggctt cgttccgcgg gttgctcgcg ccggcgcggg agcagctgac gggactgtgg
     4141 ctggtcgatg gtggcccaag ccgcgacatc atcgcggttt tcggcggcgg cacgacgatg
     4201 aaggtgatct ttgcgctggt gtggcttgcc gctgcgattg tgttctgctg gcgcaaccgc
     4261 attccagcgc tgatcatcgc cggcggcatc ggcaccgggc tggccgtcgc ggccggctgg
     4321 tggttcacct atgcgctggc acagagcgcg ttctcgccgg tgccggtcaa gagcctgacg
     4381 ttttcggggc cctcggcgga tgtgctgatg ttcgtgctct ccggcacgtt ggccaacgtc
     4441 aatttcgaca tagcgctggt tcccggggtg ttcatcggct cgtttcttgc ggctgccttt
     4501 gccggtgaac tgaagatcga aggattcacc gacggcctca gcatgcggcg ctacattttc
     4561 ggtgcgacgt tgatgggttt tggcgcaatg ctggcgggcg gctgtgcagt cggggccggg
     4621 gtcagcggcg catcggtgtt cgcgctgacc gcctggatcg tgctggtcgg gatgtggctg
     4681 ggcgccgctt tggccgattt ggctttcgat cggccgcgcc acgccccggc ggtgcagcgt
     4741 ccgtaagcgg acgctacggc gaggtcagag cgtgccttcg atcagatcct cgatgccgag
     4801 cgggctggtg tcgccgaccg agcgcccggt ttccttcgtt cccttgaagc cgatcaacgt
     4861 gctctgcttg ctggccttca tcgcgcgcag gatatccttc tcgctgtcga aatcgacctc
     4921 cagcaccagc acattcttgt acttggcttc gccaaccagc ttggcaatga tcggatcctg
     4981 caccttgcag gtcggacacc agggcgcatt gatcaccacc aggattgacc tcccggcggc
     5041 ctgcgcctgc tggaagccgg cctgggtgaa tttcgtaaag ccggccgccg cggcgtaacc
     5101 cggcaataac ggcacgacaa ggcaagcatg gaacagcgtg cgtctgttga accgcatggg
     5161 accctctcct cgatcgcttc gtcggctgtg ctcaggtgag tcaggccgac gagcgcaatc
     5221 tgcttcaacc aaacatgtcg gacacaattc cggcatacca acccatattt tccgcctgcg
     5281 tctgcttgcg caatgcggca tcctcgccgg tcttggagat gtaggtctcg gtggcgaaga
     5341 tcttgccatc ctttggctcg tatgagccac cgaccttcac ggcatcgtcg gtttcgatca
     5401 ggctccagca ggtattggca tagcgggccg gaaaagcgcg cgaaccggtg agctcgctgc
     5461 ggatgatgtt ggccgcaacc ttggcctggc tgttggccgc aaacgccgac tttggcatgt
     5521 cgcccggaat gcaggcatcg ccgacgatga aaatattggg atcgcttgcc gatttcatat
     5581 tggccggatc aatcgggcag aagccggact ggttggtcaa ttgcgcctca cgcgcgatct
     5641 tgccggccat ctgcgccggg atcacattga ccagagcaac atccttgtag gtctcaaaac
     5701 cggtcaccac cgtgttggtc tttggatcga ccgatttgat tccgtcatgc accttggggc
     5761 caatccattc gaccatgccc ggatagtgcc gctcccagcc ttcctggaac aggccctgct
     5821 tggagaagga ttccttcgga tcgagcacga tgatcttgca cttgccgcgt ccggtggatt
     5881 tcagcagatg cgccatcatc gagacgcgct cgtagggtcc gggcgggcaa cgataggggt
     5941 tgggcggagc gatcatcacg atcacgccgc cgtccttcac ctcgtccagc cgcttgcgca
     6001 ggatctgcgt ctgcgggccg gccttccagg catgcggcat gatctcttcg aattcctgac
     6061 cccagcccgg gaccgaatcg tatttgaggt cgatgccggg cgcgatcacc agccggtcgt
     6121 aggagacggt ggcgctgtcg gtcagatgca ccactttggc cgcccgatcg atgaaggctg
     6181 ccgtcttgcg ggcatggttg atcgcgtatt tcttggtcag atcgtcatag gaatgggtga
     6241 tttcctggaa agtcttgtag ccgccaacgt agagattgga atggaagcag gtcacaaact
     6301 tgtctgacgg ttcgaccagc gtgacattga tcgcatcctg actgtctcgg gcgatgaact
     6361 tggccgcggt tgctccgccc ggcccgccgc cgatcaccac cacacgcggc ttggcctgac
     6421 cgatgacggc aggcgcatag aggatgccag ccagcgttgc gacgccctga agcgcctgtc
     6481 tgcgatctag tgtcatgtca cctctcctcc cgaaaatcca acagccggtg agactactct
     6541 cagcccacct tgagataagc aaaacccttg ccctgcaacg ccgctacggt agcaacgccc
     6601 gagggcacaa actgcacgaa acccgccttg cgagcttgat cgcgggcgat cttctggcgt
     6661 tcgaacgtta tcgcgcaaag atagaccttc aggccggatt tggcgagcgc ttcgacgcgc
     6721 tcgaagatct taggcacggt tgtttcctct gcccagggcg ttccttccag cgattcaagg
     6781 aagaacttga tgcctgcgcc atgggcaacg acggccagtt gcacatcgaa cggattgttg
     6841 ccgtagaccg aaaaatggtt gccgatgttg accagcatct ggccaaaccg cgcgggatcg
     6901 ccgaaatcgc aatgatagag gcaggccgcg tccgcttcct tcttgatgtc ggcgagcgcc
     6961 agcgtcgcag gggccgcctc ggcacccgcg cccatcatcg tcccggcggc agccagtgcc
     7021 ccgatcagat ggcggcggtc gatggcaggc cctgtgtctc ggccggtgtc tgtcatttct
     7081 ttgcctccgc aatcggcttc aggctgccaa aatagcttgc gagcccgtca atgtcctcct
     7141 gagacagccg atgcgacagc gtgcgcatcg tctcgttgtc gcgcgtgccg tccttgtagg
     7201 actggatgac cgccacgaac tgatcttcag gccagccgac aatcgccgga atgcccccga
     7261 cctgacgacc cgaaacctga tgacaggccg tgcacatgtt cgacagatac tcgcccagtt
     7321 cgcggtcgcc cttgcgggca gcggtctgcg catgcgccgc agtgaccagg agaagggtca
     7381 tcagcatgct gaaaaacgtg gctttcgcgg tccccatcaa tccacctttg gcccgtcctt
     7441 ggcgtcgggg gtgacgtcga tcacccgagc gcgccccgtc accttcgccg ggtcaggctt
     7501 gcatgctttc atacagggtt cagcccagaa atgtctttcc gttttctccc ggtcgtcgtc
     7561 gaagaaccct tcaccgttgg gcagtttgac gcctgtgaag ttctccttgg acagaacgaa
     7621 atcgtccttg accacatcat tgaggctcag cacataggcg gtgatcgcat agatctcgtc
     7681 gtcgctcagc gaccgcgcat tgccataggg catggcacgg cggatgtagt cgaaggtggt
     7741 cgaggcaaac ggccagaacg agccgaccgt cttttccggc ccctcgttct tcagtgatcc
     7801 gcggccgccg gcgagcaccg gccagcgtcc ggcgctttcg ccgaactcgc cgtggcagga
     7861 ggcacattgg gcctgataca gctcttcgcc ctgtttgacc gaacccttgc cgggcggcag
     7921 gccaagacca tcagggcgca catcaatgtc ccaggccttg atctcatcgg gatgagcctc
     7981 gcggcccagc ccaaccttct tggccggagc cggagtggcc gcaggcgacc ccgtctttgc
     8041 cggtgcctgc ccctgcgccg aaaagctcag catgctgaga gcaactccgg cgccaaatgc
     8101 tgccgccatc accttacgag atttcgacat tttccgtctc gccgttggaa cgcaccagcc
     8161 aggtctggat gccgttgttg tgatagatgg agttgacccc gcgcaccttg cgcagctcat
     8221 ccttggtcgg ttgcacatac cccgtcgaat ccatggcacg cgactggatc atcatttcgc
     8281 gaccgtccca gtcgaagtcg agatagaacc gggtcagcga cagcggcagc accggtccat
     8341 cgatacgcgc cgttgtccag ttgcgaccgc cgtcggtcgt cacatcgacg cgctggattg
     8401 tgccgcgccc ggaccaggcg agcccggtca gcacgttcgg ccccttgtgt ttcagcggtg
     8461 cctgcggcga gggattggtg accacggatt tcgcatccat gatccaggtg aagcgccgcg
     8521 accggccatc agcaagaaga tcagtatact ttgaagtttc ctcgcgatgg tgccagggct
     8581 gatcgccgac ttcaatacgg cgaagccact tcacccacat gttgccttcc cacccgggca
     8641 ccacgatgcg caggggatag ccctgctcgg ggcgcagcgc ctcgccgttc atagcccagg
     8701 cgacgaggca atcgttcatg gccttttcga tggggatgga gcgggtcatg gcggcggcat
     8761 ccgccccttc cggcatcacc cacttgccgt tggttttcac ccctgcctcg tccagcagcg
     8821 ctttcagcgg aataccggta taccagacgt tatggatcat gccatgggtg aactggcagc
     8881 cgttgagctg ggcgccgcgc cattccatgc cgccgttggc ggcgcattcg aggaagtaga
     8941 gcctgttgac gcggccgggg aagcgcttga gatcctcaag cgtgaacacc agcggcttgt
     9001 cgaccagccc gttgatcatc agtcggtgct tggcaggatc aacctccgcg acgccggagt
     9061 gatggcgctc gaagcatacg ccattgggcg tcacgatacc gtcgagcgca tggatcggcg
     9121 tgaaattgat ggatgaggcg gtgtctgccg tcagccatgg cacattgcgg cgcacgacat
     9181 gcgcctcatg cttcgatggc cggccatagg gcgccgcctc gactccggga ccgagatagc
     9241 ggttccagtc ctggatttcg gtgatgacct tttcaggctc agccgcaccc gccgggccgg
     9301 caaaggcccc ggcggcaacg ctggccccgg cgaggccggc gccgcgcagc agccctcgac
     9361 gggacagcgg ccctgcggtt ttcatgtcgc tcatggcaag tctccctcag gcaagtcgcc
     9421 cacggattgg gtcaggcgcc ggtgactttc accgacgtat tgggttcgag ccgcacggtc
     9481 ttgacgcgcg acacatagcg atccaccacc tcccagatcg gcgggccggc gacgttctgg
     9541 gcgacgctgg cccatccggc gacgacatat ttgcgcgccg catcgatcgg ctgaccgctc
     9601 ttgaggtggg taaggtcgga aatgcgctgg ttgatcggct tggaaatgtc gatcgaatag
     9661 ccaacaccgc caacccgcac catgtcgcca ccgccctgga aatagggatc cgggtggaag
     9721 atgttgtcgg caacatcctc gagcatgagc ttgatctgct cgccggtcat ctcgttgcga
     9781 taacagttgg gataggtcat cgcggtggcg ttggcgatcg cctcgaaggt gatcggatcg
     9841 cccggcagca gcgaggcgcc ccagcggaac cccggcgaca gcgaaatctc cgcgtcccgc
     9901 tcggccagca tcgcctggca gatgaggtcg tcgaacgtgc cgttgaaatt gccgcgccgg
     9961 tagagcaggg aatcggtctg gccgacgatg cgggacaggt ccgcctgata gggcttgcgc
    10021 cacttgtcga tcagcccggt catcgccgcg tcggactgga tcgcgtcgga aaacaccggc
    10081 atcagcttgt agcgcagccc ggcaaccttg cggtccttca cctcgatgtc gaggcgcgag
    10141 acgaacttgc cgtgcgagcc cgaggcgacg atggctgttt cgccaacctt tagtacaccc
    10201 ggcatcgcgt cgtgggtatg ggcagccagc accacgtcga tccccttgac ccgggaagcc
    10261 agcttcttgt cgacatcgac cccgttatgg gacagcagca cgacgatggc agcgccttca
    10321 ccgcgcgcat cgtccacctg cttctgcaat tcgtcctcac gcagaccgaa ttcccagtcc
    10381 gggaacatcc agttcggatt ggcaatcgcg gtgcgcggca tcgcctgtcc gatcacggcg
    10441 atcttgacac cgcccttctc gaacatcttg cgcggcttga acacctcctc ctgccattcc
    10501 ttggagcgga tattctgggc aaggaaggcg atggacgagg cttcggcgat ctccttcacg
    10561 cgcttctcgc ccagcgtgaa ctcccagtgg gaggtcatcg catcaacctt gagcgcttcc
    10621 atgatctcga ccatgtcggc ggcatcggtc ttgagcgcgg accaggagcc ctgccaggta
    10681 tcgccgccat cgagcagcag aaccttgtcg tcgccgcgtt cggaccgcac cgccttgatc
    10741 agcgtggcga cgcggtccat gccccccatc cgcccgtagg tcttggccag cgacacaaag
    10801 tcgtcggccg tcaaagcatg ggcgtcggcc gagccggtgg cgatcttgaa gtgctcacga
    10861 aacgcctggt cggtcagatg cggcggcacg ccacggtcgg cgccaacccc gagattgacc
    10921 gacggctcgc ggaaatacac cggcatgagc tgggcgtgga tatcggtgac atgcaggatt
    10981 gtcaccgtgc cgaggggctc gaacttcagg atgtcggact ggcccagctt ctgctgcgcc
    11041 gcgacgcggc cgagcgagcc aaccccggtc gcaggtaata gcgcgcacgc agccgctgtc
    11101 gcctgcagga aatgcctgcg cgagatcatg tcagaccttt ctggatgcgg gaaaagagcg
    11161 gggccgggcc ccgccctgac gcttaggcca ccgtcagctt ggcggtcgcc gtatagtcgg
    11221 agccgttgtc gtccttccag gtgaacttga attcacccga ttccttggcc ttgaagaaga
    11281 acgactggta cggattggcc gagatggccg ggtgccagtc cgcctcgaac accgtcttgc
    11341 cgttgaacac agcggtgaac ttgttgatga tctggcgcgg aatggtcttg ccggccgaat
    11401 ccttgcgctg gccgctttcc atctcgtggc tgatcagcgt cttgatctcg atcatgtcgc
    11461 cggccttcgc ctgggcgggc acacggacgc gcggaacgga attggtcgcc atggtgtcct
    11521 ccctgtgatc agccgccgca tccgccgatg gtgaccttca cctcggcctt ggcggaaaac
    11581 aatgcgccgt tcgacatttc ggcaacgcag acgatgttct gcgtctgggc tagacgcatg
    11641 cgcgccgacg cattggcctt gccgcattca ggcgagaacc ggaagctggc gatgcccggc
    11701 agcgggtttc cgtcagcgaa cacatgcacg gccttgacga aatcggcgtc ggtcatcggg
    11761 ctctcgacct cgaccgtgat cggcacgacg agtccattct cggcgatctg cggcacgtcg
    11821 agcttgatct tgcctgaatc gatcttcttg tcgccataca gcttcttgat ctcatcctcg
    11881 accgccttga catcagccag cgccagccgg ggcgcaagca tggtcgcaaa tcctgcgacg
    11941 gcggcagaca gcataagatc acggcgggac tgcaatcggt gggccgatgc catatccttt
    12001 tcctcctcag tcacattctc tgacttcatg cgccggttcg cgcaaaaagc ctatagacat
    12061 tttcgtcaac gcatatatcg aattatatga atgtaaagat caaaaaggca ggcagttccc
    12121 attttccgcc taaagggtga taaggaacga ctgcagatag cacgagggag gtgtcggcat
    12181 atgaagcgaa cgagggggaa atgggccgct ttcgggctgg ctgtcgcggc ggttgcgctc
    12241 gcggcaggca tggcacctgg catggcccag caggacgacg cggaaaagga gatcgaacgc
    12301 taccgcgcga tgatcaacga tccgatggcc aatccgggct ttctgtttgt cgatcgcggc
    12361 gaggcgctgt ggtcgcagaa gcgcggcgcc aagaacgtct cgcttgagac ctgtgatctt
    12421 ggcgaggggg caggcaagct ggaaggcgcc tacgcaaagc tgccgcgcta tttcaaggat
    12481 gcggacaagg tcatggatct tgagcagcgt ctgctctggt gcatgaagac ggtgcaggat
    12541 ctcgacacgg ccgatgttct caagcgccgc ttcggctcac ccagcaatac ctccgatctt
    12601 caggatctgg tggcgtttat cgccaacaag tcgagcggca tgaaatacca gccgcagctc
    12661 gaccacccga aggagaagga gatgctggcg gtcggcgagg cgctgttcta ccggcgcggc
    12721 ggcagccagg atttctcctg ctccacctgc catggcgaat cgggcaagcg catccgtctc
    12781 cagggacttc cggacttctc caagcccggc aaggacgcgc gcgagaccat gggttcctgg
    12841 ccgacctatc gggtttcgca gagccagctg cgcaccatgc agcatcgcct gtgggactgc
    12901 tatcgccaga tgcggcatcc ggcgcccgag ttcggctcgg atggcataac cgcgcttacc
    12961 gtctacatgg tcaagatggg cgagggcggc gagatcaccg tcccgtcgat caagcgctga
    13021 ggagggcgac cgatgaagaa aaccatcctg gccgcccttc tgtgcggact tgccggcgct
    13081 tccttggccc agcaggcaaa ggttgatccg gctgccgttg aggctgccgt gaagggcatg
    13141 tgggcgaagg ccggccccga atgggcctcc tcgatcacgc ccgacgagac gatgaaagtc
    13201 tgttcggaga cgcgcaatgc gccgtccgcc gccgagttcg agaagatcgt cgcgcgcgag
    13261 aaggccaccg tggtgtttcc ggcggacggc aacgtcatgg gcgattggaa gaagggcgaa
    13321 atcgttgcct cgcgcggcac cggaggccag ttcactgaca cgccggacac ctatcgcggc
    13381 ggcaattgct acgcttgcca tcagctcgcc gccaaggaag tcagctacgg caccctcggc
    13441 ccgagcctgc tcggctatgg caaggcgcgc aacttctcgc aggaggaggc caaggttgcc
    13501 tatgccaaga tctacaatcc gcaaggtgtc ttggcctgct cgcagatgcc gcggttcggg
    13561 cactggaagt tcctcagcga ggagcagatc aaggacgcgg tcgccttcct gtttcacccc
    13621 gattcgccgg tgaacaagta acgccggagc aaaccggttt ccggccggcg ccggttgcgc
    13681 cggccacctg caacaacagc aaggggcgcg cgccccttgc acactggagc attttcaagc
    13741 gaagtggagt ccggttcgcg tgaagaaaat gcgacaaccg gaaaccagag cattttcaag
    13801 cgaagtggag tccggttcgc gtgaagaaaa tgcgataaag caagaaattg gagcattttc
    13861 ggtgaaattg aattcaccga taagtgctct agtggggccc gcgatgcgca aggccatttt
    13921 cggcgcggca ttggccgcat gggtatctgt tctgaacccg cccgctggcg cggcagaact
    13981 tgtcatggtc gagcgcgatg attgttcctg gtgcaagcgc tggcatgccg aggtcggtga
    14041 atcctatatg aaggccgagg aagggcggca ggcgccattg cgccgctggc ggctggagca
    14101 gggccagccg aaactggcca tgaaggagcc ggtgcgcttt accccgacat tcattctggt
    14161 agaggacggt cgcgagatcg gccgcatcac cggctatctt gaaaacggca tgttttgggg
    14221 actactcggc gatctgctga agaaggccca atcccaacaa gcgaggtaac ccaccgtggc
    14281 gcctgcgacc tctgcatgga gcgacgaact cgaagccgaa ctcaagtcgg atatcgagac
    14341 ctcgcccgag attgaagcca tgatgcgcaa tgcgcgtgat gccagcgatc ttctcaaggc
    14401 tctgtcccac gagagccggc tgcttttgct gtgcattctg gctgaaaagg agcgttcggt
    14461 cggcgaactg gagacgatcc tgtcgctgcg tcagcccacg gtgtcacaac agctggcccg
    14521 cctgcggtat gacaatattg tcaccacccg ccgcgacggc aagacgattt attattcgat
    14581 cgccaatgac gacctgcgca agattgtgcg ggtgatctac gatattttct gtgtttctcg
    14641 cgccgcgcgc tgagccttgg ccagggcggg tgacgaatgg atagcctctt cgggtccccg
    14701 gttccggcag ggtttctcat cggcgtgatt gccggctttt tcgtccggcg cgcccggctg
    14761 tgtagctttg gagcgattga atcggcgttg atcggcggtg actggcgacg catgaaggtg
    14821 tttggcatgg ccttgggggt tgcgctggtg ctgacccaga ccatggcgct gaccggcctg
    14881 atcgatcccg gccattcgac ctatgtcaat gcccgcatcc cgctgctggc cacggctttc
    14941 ggctcgctga catttggtct gggcatggcg ctggtcggca cctgtgcctt tggctcgctg
    15001 gtgcggcttg gaagtggcga tttgcgcgcg ctggtcacgc tgatggtgtt tggcgcactg
    15061 tcctatgcga cgttgcgcgg agtgctctcg ccgatgcggc tttcgctgtt tgaggcggtg
    15121 accatatccc tgcccggtgc cattgccgga acgctggagg ccttcgcggc ggcaatggga
    15181 ggcgcactgg cgcgtgtcgg gttgatcggc ctgctcgcgc tggggctggt cggggcggcg
    15241 ctattggatc cgcggctgtg gcgggcgccc aagctgatga cggc
//
LOCUS       DAOMXG010000043        15217 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_2166_length_15217_cov_10.624654, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000043 DAOMXG010000000
VERSION     DAOMXG010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 15217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15217
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2166_length_15217_cov_10.624654"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="PLQ11_04645"
     CDS             <1..317
                     /locus_tag="PLQ11_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018045162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRK24224.1"
                     /translation="LLAAVGLRHRLRHYPAQLSGGEQQRVALARALAPEPAILVADEP
                     TGNLDEATGRQIIDLMFALRRERGATLILVTHDMALAGRCDRTIRLRSGEIVIGTAQA
                     AE"
     gene            314..2878
                     /locus_tag="PLQ11_04650"
     CDS             314..2878
                     /locus_tag="PLQ11_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018261292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRK24225.1"
                     /translation="MTPALSFAGRMRLALRLAARDLRGGIGGFGIFIACIAIGVAAIV
                     SVSVLSRSLQDGLAREGRTILGGDISFSFLQREATPDERAFFSRTGSVTAFAFLRAMA
                     RSDTGAATLVELKAVETGYPAFGRLVTRPEQPFQSLFGAGDGLFGVAVDPLLLGRLGL
                     GVGDSLWLGTARFVIRAEIDSEPDRLALGVGFGPRVLMSREGLDASGLVQPGSLTRFA
                     YRIALPGSPPDTASLDTLTDEARRAFPGAGWDIRTRDNAAPQLKRNIERFTQFLTLVG
                     LTSLAVGGVGVANAARAFLDRKRLTVAALKGIGAPGGVMVLTLVVEVLALGLIGTLVG
                     IIAGTGLPILVIHFLAAALPVPLASDLYPREALVGFIYGLATVLAFTLWPLGRAHDIP
                     VSALVRDHFEDDRAWPRRRYMLATALAVLLLAGVAIAFAWDRRIAIIYVAAAAGAFVL
                     LRGVAAAIMALARRLPRPRSTEARLALVNLGRPGALTPSVILSLGLGLTLLVAMGLID
                     HTMRAQLSRSLPDKAPSFFFLDVPAADTDAFTALVAELAPGATLNRVPMMRGRVTALN
                     GTAAETIRANDKVAWVLEGDRGLTFSAEIPAGNVLAAGQWWPADYAGPPLVSLALDIA
                     EGLGLSIGDSITVNVLGRPVTARIANLRKVEWQNLGISFVLVFSPNTFAGAPYSNLAT
                     LTYPASVGPDAELKVLRAVAERFPSVSSIRVKEALETIDGIVAKLALAVRAASAVAIL
                     SSIIVLAGALAAGRRVRAQEAVILKTLGATRPRLILAMILEYALLGLSAAIFGLLAGT
                     LAGYAIVTQVMNLTFSLPVSSMLTLVLTTLVLVVILGLIGTWRVLGQKPASYLRNA"
     gene            3084..3842
                     /locus_tag="PLQ11_04655"
     CDS             3084..3842
                     /locus_tag="PLQ11_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002965336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HRK24226.1"
                     /translation="MSNYDRNAYAPYGQVATQAQIDQGLRTYMLGVYNNMVLGLAITG
                     VAALGIFMLSRAGVTPTGRPIMSDFGKLLFGSPLKWVVAFAPLAVVMFLSFRIHAMSA
                     AAARATFWLYSALVGTSLAVLFFIFTGQSVVRVFFITAASFGALSLWGYTTRKDLTGM
                     GSFLIMGLFGLMLASIVNIFLGSTGMQFAISLLGVLIFAGLTAYDTQKIKEMYFEGHA
                     PDMAAKLSIYGALTLYMDFLGMFQMLMSLLGDRE"
     gene            complement(3910..4227)
                     /locus_tag="PLQ11_04660"
     CDS             complement(3910..4227)
                     /locus_tag="PLQ11_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2794 domain-containing protein"
                     /protein_id="HRK24227.1"
                     /translation="MATYRQPERVAFSRAELQQILVVYGRKVAQGEWRDYALEFGREA
                     ATFSIFRRSSEQPLFRIVKTPKLAGKQGAFSVVASAGYVLRRGHDLARVLMVLEKPTK
                     VAP"
     gene            complement(4552..5121)
                     /locus_tag="PLQ11_04665"
     CDS             complement(4552..5121)
                     /locus_tag="PLQ11_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009452321.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HRK24228.1"
                     /translation="MQNPAAVTALSLAPAEATPGVAALFDKCVEKLGFVPNVLLAYAH
                     DMAKLEAFSAMYNDLMLAPSGLSKLEREMIAVVVSAENRCFYCLTAHGAAVRQLSGDP
                     VLGEQLVMNYRSAALPERQRAMLDFAVKLTRAPYAIEESDRAIVRVAGFNDRDIWDIS
                     AVAAFFNMSNRMASAVDMRPNAEYHRQTR"
     gene            5259..10040
                     /locus_tag="PLQ11_04670"
     CDS             5259..10040
                     /locus_tag="PLQ11_04670"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HRK24229.1"
                     /translation="MAKTGNGSGAMNSLLHSTFEALLYGRVVPEDRAVYPPGALATIA
                     AGALERFGQRKAGGADIHLTDVDGPDGARITVIEAINDDKPFLVASTLAELNERGLDV
                     RLVAHPVLAVRRNDKGELTSLGGLAGAGPDPDFVRESFVHIHVARIVGKAAREALVRS
                     LQEIYRDIGLAVHDWKAMRERLAGVAHRYREHPPMLPADEIAEAVQFLDWLAADNFSF
                     LGMREYRFASLASEPVAGAGLGILSDPEVKVLRRGSELVVTTPEIRAFLSEPSALIIT
                     KANVRSRIHRRVHMDYIGVKLIDETGALEGELRLIGLFTSTAYTGSTRTIPYIRHKVA
                     QVMMKAGFDPASHSGKALMNVLESYPRDELFQLDRDTLYGFAMDAMNLNERPRIRALV
                     RADRFDRFVSVIVYVPKDRYDTGVRLRIGDFLARSFDGRVSAAYPSYPEGPLSRTHFI
                     IGRHGGATPKVERSTIEAGIAAVVRTWGDTLKTTIETTHAPDVAETLLARYGSAFSAG
                     YREAFAPAEALADIAILENLSAEQPTAVDLYRAPQAEAGTARLKVYAFGAPIALSRRV
                     PVLENLGFSVSNERTYDLHLPMTAGKAAAYLHDMTIARARNGNIDVDVMEERVEAALM
                     AVFADQTESDRFNTLVVEAGLTWREAMVLRAYGRYLGQAGAPFAQNYLADVLAKYPEI
                     ARGLIELFGARFDPERPGDNTARIAAGTAVADRLRAAMDAVSNLDEDRILRRFLNLIE
                     ATLRTNYYQRLGSSEMRPALAFKFDPRRIDGLPLPRPQFEIYVHSPRVDGVHMRFGKV
                     ARGGLRWSDRPMDFRTEILGLVKAQHVKNSVIVPVGAKGGFVPKRLPPPSDREAWMAE
                     GIAAYKTYINALLDVTDTISGRGIKGPAGVVRLDGDDPYLVVAADKGTATFSDIANAI
                     SIERGHWLGDAFASGGSQGYDHKKMGITARGAWECVKRHFREMDIDIQAQPVTVAGVG
                     DMSGDVFGNGMLLSKALRLVAAFDHRDIFLDPDPDPARAHAERQRLFDLPRSSWQDYD
                     RALISKGGGIYSRSAKEIVLSPELRATLKIEAERVTPQELMMAILKAPVDLLWFGGIG
                     TYIRSGEEADVGVGDRANDAIRIPATALNCRVIGEGANLGVTQRGRIEASRRGIRLNT
                     DAIDNSAGVNTSDVEVNLKIALAMPVADGSLGETERNSALAGMTDEVAALVLRNNYLQ
                     SLALSLAERRGAAGLAELQRLMQGLESRARLDRAVELLPADTEIARRIAAGEGATRPE
                     LAVLLAYAKLSFFDDLIVSEVPYDPYFADELDRYFPLAVRGRFPEAVKNHRLRREIIA
                     TQLANIVVNRCGPGGIARLQDETGADIPSIAAAYALTRDAFGLLDLNGAIDQLDTKIP
                     GAVQLDLYAALQDMLVGRLAWVLRNVDLTRPLGPEVRRFKAGIDASRALAPQLITPAQ
                     NQRRSERLAEWTGKGVPQTLAMSIVDLPALVTAPDAVLVAEQTGKPVRDVLETLFALA
                     DRFNIGRIRQAAATIPARDRFERLAIDRAIDGMDLSLRRLAGEVIAGYGPGMAGVATF
                     AAKRGRAVERITAAIEEIVASGLTQAKLTIAANLLGDLVRS"
     gene            complement(10054..11646)
                     /gene="purH"
                     /locus_tag="PLQ11_04675"
     CDS             complement(10054..11646)
                     /gene="purH"
                     /locus_tag="PLQ11_04675"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HRK24230.1"
                     /translation="MPQTLRPLRRALISVSDKTGLAELGQALGQRGVTLVSTGGTYQA
                     LKAAGCAVTEVADITGFPEMMDGRVKTLHPKVHGGLLAQRGHPEHEAALLAHDIPPID
                     LLIVNLYPFEATVAAGKAYADCIENIDIGGPAMIRAAAKNHQDVTVIVDVADFAALIA
                     ELDAQNGATSFAFRQMLAAKAYARTAAYDAAISTWFAAELEDRTPTYAAIGGRLVERM
                     RYGENPHMAAGFYRAGESRFGVATARQVQGKALSFNNINDTDAAFELVAEFDPRRTAA
                     VAIIKHMNPCGVAEGASLLDAYQRALRCDPVSAFGGIVALNRTLDADAARSIVEIFTE
                     VIIAPDASDEAIALIKAKKNLRLLLTGGLPDPRAEGRMIRSVAGGFLLQDRDNAVVDD
                     MNLKVVTRRAPSERELMDLKFAFRVAKHVKSNAIVYARDGATVGVGAGQMSRVDSARI
                     AAWKADEAAKAAGLSESLAKGSVVASDAFFPFADGLLAAAAAGATAVIQPGGSMRDNE
                     VIAAADEAGLAMVMTGIRHFRH"
     gene            complement(11759..13447)
                     /locus_tag="PLQ11_04680"
     CDS             complement(11759..13447)
                     /locus_tag="PLQ11_04680"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HRK24231.1"
                     /translation="MTIGLGTPRPPASGGMRGRFGLGTLWRNFRLKVPERLLIAPQEI
                     ETGDPTVARDIYAGSYTLAGRTVLTRGQSPFSVEAPHPAWLRELHSFAWLRHFNESEE
                     PVIRVHARALVDDWLLNPTLAQRPAAQRSDVLAHRMTAFLVNAPLLLTGADHDFYVRF
                     MRALAGQAKALERAAERDRDWIARAEAAIAFGLYALCALTPERTLKRAVRLLERSLSM
                     VQAGDGGPVDRNPNSALVLLAMLLPLRTAFSARNNRPPPVLETTIDGLLRFLMFLRHP
                     GNDIALFNGMSASQLTVIGSLIQFLDSTSESFPVTGMCGYHRLAVGRSVVLVDAGPCP
                     PSGMTARFHAGATSFELSSGHERIVVNCGAAPAGLAGMREALRETAAHSTLDLEGISR
                     LSFSDALTADGEAERRASARQRAPAVERRVEADGDSVDMTDRCYEATHGLVHRRQLTL
                     TAEGSYLSGRDSLIGLSAGGGVRATARLRFHLHPQVRAFLSGDGRAVEIQLASGESWT
                     FSAGGRSLALEDSIFFGGLASQRRCQQITVALDGNEPEVVWSFARRSGGRLVMS"
     gene            complement(13534..14859)
                     /gene="rsmB"
                     /locus_tag="PLQ11_04685"
     CDS             complement(13534..14859)
                     /gene="rsmB"
                     /locus_tag="PLQ11_04685"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020095354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HRK24232.1"
                     /translation="MTRPEARQSVAVDARDFAVRAISDVVRRRLPLDDVLDRFSKDPV
                     FEALAPADRGLARAIAVATIRGLGYLQHCLDERLKDGLPPQSGPLQPLLLAGLAQLLF
                     LDVPDYAAVDTTVAKLRADRIGQRYAGLANAVLRGVARDKAALLARLDPLTHNTPEWL
                     AARWRRHYGEDVARSIAAAHLVPPPIDLTVRADAITWAERLGGVRLPTGSLRLNSGQR
                     VPDLPGFSEGAWWVQDAVAALPARLLRVEPGQRVLDLCAAPGGKTAQLAAMGARVVAV
                     DRSPQRLKRLEANLARLGLAAEVHAADGGAFSQGGFQRILLDAPCSATGTIRRHPDVA
                     WNKTLADLAALIAVQRRLVDHAWTLLEPGGVLVYATCSLEPEEGEEQIARFLADHSDA
                     ELDPIGAAEAGDCAEMITAGGLMRALPCHLGTFGGCDGFFSARLRKQRP"
     gene            complement(14856..>15217)
                     /locus_tag="PLQ11_04690"
     CDS             complement(14856..>15217)
                     /locus_tag="PLQ11_04690"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HRK24233.1"
                     /translation="MRIADPGDREGKAAITRYRVLAAGDGLSLVQFEPQTGRTHQIRV
                     HSAALGCPIAGDVLYGGDRARALARQLQLHARQIVLPSREGVAAVTVTAPLPDHMRAL
                     VGLIGLDPDAILVEPKA"
BASE COUNT         2606 a         5047 c         4743 g         2821 t
ORIGIN      
        1 agctgctggc cgcggtaggc ttacgtcatc ggctccgcca ttacccggcg cagctgtccg
       61 gcggcgagca gcagcgcgtc gcgctcgcac gggcgctggc gcccgaaccg gccatcctgg
      121 tggcggacga gccgaccggt aatctcgacg aggcaaccgg ccgccagatc attgacctga
      181 tgttcgcgct caggcgtgag cgcggcgcca cgctgatcct cgtcactcat gacatggctc
      241 tggccggccg ctgcgaccgc accatccggc tgaggtcggg cgagatcgtc atcgggactg
      301 cgcaggccgc cgaatgaccc ccgctctgtc gtttgctggc cgcatgcggc tggccttgcg
      361 ccttgcggct cgcgatctca gaggcgggat tggcggcttt ggcattttca tcgcctgcat
      421 cgccatcggc gttgcggcga tcgtgtcggt ctccgtcctg tcgcgatcgc tgcaggacgg
      481 ccttgcccgg gaagggcgca ccattctggg cggcgacatc tcgttctcgt tcctccagcg
      541 cgaggcgacg ccggacgagc gcgccttctt cagccgcacc ggctcggtga ccgcctttgc
      601 cttcctcagg gccatggcgc ggtcggacac cggcgcggca acattggtcg aactcaaggc
      661 ggtggagacc ggttaccctg ccttcggccg cctggtgacc cggccggagc agccgttcca
      721 atcactgttc ggtgccggcg atggcctgtt cggcgttgcc gtcgacccgc tgctgcttgg
      781 ccggctcggc cttggcgtgg gcgattcgct ttggcttggc accgcccgtt tcgtcatccg
      841 cgccgagatc gacagcgagc cggaccggct ggcgctgggc gtcggcttcg ggccgcgcgt
      901 gctgatgtcg cgcgaagggc tcgatgccag cggcctggtc cagcccggca gcctcacccg
      961 ctttgcctat cgcatcgccc ttccgggctc gccgccagac accgccagcc ttgacacatt
     1021 gaccgacgag gctcgccggg ccttccccgg cgctggctgg gatatccgca cccgtgacaa
     1081 tgccgccccg caactcaagc gcaacatcga gcgtttcacc cagtttctca cccttgtcgg
     1141 gctgacctcg ctggcagtgg gcggggtggg cgttgccaat gccgcacgcg cctttctcga
     1201 tcgcaagcga ctgaccgtcg ccgcgctcaa gggcattggc gcgccgggag gcgtgatggt
     1261 gctgacgctg gttgtggaag tgctggcgct tggcctgatc ggcacgctgg tcggcatcat
     1321 cgccggaacc ggattgccga ttctggtgat tcatttcctt gctgcggcct tgccggttcc
     1381 attggcgtct gatctctatc cgcgtgaggc gctggtcggg ttcatctacg gccttgcgac
     1441 ggtgctggct ttcacgctct ggccgctggg ccgggcccat gacatcccgg tctcggcact
     1501 ggtcagggat catttcgagg atgaccgcgc ctggccgcgg cggcgctaca tgctggccac
     1561 cgcgctggcg gttctgctgc tggcaggcgt cgccatcgcc tttgcctggg accgccgcat
     1621 cgccatcatc tacgttgccg ccgcggcagg cgcttttgtg ctgctccgtg gtgttgcggc
     1681 agcgatcatg gcgctggccc gccgtctgcc gcgaccacgc tccaccgagg cccggctggc
     1741 actggtcaat ctcggccgcc ctggggcctt gacgccttcg gtcatcctgt cgctcggcct
     1801 tgggctgaca ctgctggtgg ccatggggct gatcgatcat acgatgcgcg cgcagctgtc
     1861 gcgcagcctg ccagacaagg ccccgagctt tttctttctc gatgtcccgg ctgccgacac
     1921 ggacgccttc accgctctcg tggcagaact cgcgcccggc gcgacgctga accgcgtgcc
     1981 gatgatgcgc ggccgtgtca cggcgctgaa cggcacggcg gcagaaacca tccgcgccaa
     2041 cgacaaggtc gcctgggtgc tggaaggcga tcgcggcctg accttttccg ccgagattcc
     2101 cgccggcaat gtgcttgcgg cgggccaatg gtggccggcc gactatgccg gtccgccgct
     2161 ggtctccctc gccctcgaca tcgccgaagg gctgggcctg agcatcggcg attcgatcac
     2221 cgtcaacgtt ctcggccggc cggtcaccgc gcgcatcgcc aacctgcgca aggtcgaatg
     2281 gcagaatctg ggcatcagct ttgtcctggt gttcagcccc aatacctttg ccggggcgcc
     2341 ctattccaac cttgccacgc tcacctatcc cgcatcggtc ggcccggacg ccgagttgaa
     2401 ggtgctgcgc gccgtcgccg agcgctttcc ttcggtatcc tcgatccgcg tcaaagaggc
     2461 gctcgaaacc attgatggta tcgtcgccaa acttgcgctg gcggtccgcg ccgccagtgc
     2521 cgtggcgatt ctgtccagca tcatcgtgct cgccggagcc ctcgccgcag gccgtcgcgt
     2581 gcgggcccag gaagcggtga tcctgaaaac gctgggcgca acccgtccga ggctgatctt
     2641 ggcgatgatc ctggaatatg cgcttctggg cctttcggca gcgattttcg gcctgctcgc
     2701 cggaacgctg gccggatatg ccatcgtaac ccaagtgatg aaccttacgt tttcgttgcc
     2761 cgtctcgagc atgctgactc tggttttgac tacgcttgtt ctggtcgtta ttttaggact
     2821 aattggaact tggcgcgttc ttggccagaa accagcctca tacttgagaa atgcttgaga
     2881 aacgctctac ctagtttcat aagagatcga cacgctattc ttgtccccta ccaatttaaa
     2941 ccaaaaaaac gagggttgag acagaaacgg cacgtctgct cttgaaaact agtcataact
     3001 tggccatatt ggccgggtca tagtgttggc tgacggacgc tggaggagcg ttgccagcgg
     3061 ttcaaccgat cgagagggtt cacatgtcga attatgatcg caatgcctac gcgccgtatg
     3121 gccaggttgc gacccaggcc cagatcgacc agggtctgcg cacgtatatg ctcggcgtct
     3181 acaacaacat ggtgctgggg cttgccatca ccggcgtcgc cgcgcttggc atcttcatgc
     3241 tttcgcgggc cggcgtcacg ccgaccggtc gcccgatcat gagcgacttc ggcaagctgc
     3301 tttttggcag cccgctgaaa tgggtggtgg cctttgcccc gctggccgtc gtgatgttcc
     3361 tcagcttccg catccatgcg atgtcggcag ctgcggcccg tgccacgttc tggctgtatt
     3421 cagcgctggt cggcacctcg ctggcggttc tgttctttat cttcaccggc cagtcggtgg
     3481 tgcgcgtgtt cttcatcacg gcggcgtcgt tcggagctct gagcctgtgg ggctacacca
     3541 cccgcaagga tctgacgggc atgggctcgt tcctgatcat gggtctgttc ggcctgatgc
     3601 tggcgagcat cgtcaacatc tttctgggca gcaccggcat gcaattcgcc atctcgctgc
     3661 ttggcgtttt gatcttcgcc ggcctgacgg cctatgacac gcagaagatc aaggagatgt
     3721 atttcgaggg tcatgctccg gacatggcgg ccaagctgtc gatctatggt gcgctgacgc
     3781 tctacatgga cttcctcggc atgttccaga tgctgatgag ccttctgggc gaccgcgaat
     3841 aagcgctgcc cgacgttcag aatcgaaagc cccggcggag cgttccgccg gggtttttta
     3901 atgcctttgt cagggcgcca cttttgtcgg cttttcaaga accatcagca cgcgcgcaag
     3961 atcatggccg cggcgcagga cataaccggc gctggcgaca accgagaagg cgccctgctt
     4021 gccggccagt ttcggggtct tgacgatgcg aaacagcggc tgttcggagc tgcgacggaa
     4081 gatcgagaac gtcgccgcct cgcgcccgaa ttcaagcgca taatcccgcc attcgccctg
     4141 cgccaccttg cggccgtaga cgaccaggat ctgttgaagt tcggccctgc tgaaagccac
     4201 ccgttcgggc tgacgatagg tcgccattga tacgacgacg gcgggcgcgg ccgaaacatc
     4261 actctcgctc atgagcactc ctgaccgtca ggatgattgc cgcaagccgg cctttgtgca
     4321 agtccggcgg caggaaaaag cggcgttcct cgcagtggat gcgatttccc aattttgcct
     4381 tgatttgacc attccgctgc cgttgacgcc ctctagcttt cctccatgcc aagacgttgg
     4441 ccgccggggt cggatgtaca agccccccca gcccccaccg cgctccgctg cggcctatca
     4501 gacgtcatcg gcttcggcgg tcgaccttgc ccggtcggcc gccgtttctt ttcatcgggt
     4561 ctgtcggtgg tattcggcgt tcggccgcat gtcgacggcc gatgccatgc ggtttgacat
     4621 gttgaaaaag gcggctaccg cgctgatatc ccagatgtcg cgatcattga atcccgctac
     4681 ccggacaatc gcccggtcgc tttcctcgat ggcatagggg gcacgggtta acttgacagc
     4741 aaaatccagc atcgcccgct gccgctccgg cagggctgcg ctgcggtagt tcatcaccag
     4801 ctgctcgcca agcacaggat cgccagacaa ctggcgcacg gctgcgccat gggctgtcag
     4861 gcagtaaaag caccggtttt ctgccgaaac caccacggca atcatctcgc gttccagttt
     4921 cgacagcccg gatggtgcca gcatcaggtc gttgtacatc gccgagaacg cctcgagctt
     4981 ggccatgtca tgggcatagg caagcagcac attgggcaca aagccgagtt tctcgacaca
     5041 tttgtcgaac agggcggcga caccgggcgt cgcttcggcc ggagccagtg acagggcagt
     5101 cacggcggcg ggattctgca tgatcaactc tctccagtct ggcatgatcc gggattggaa
     5161 tggtttcctc tgacgctccc gccgtcaagt cggcggtatt ggatttgctc ttggctcgag
     5221 acgaactaaa gtggcacgac acacgggagg atagtcacat ggccaagacc ggcaacggct
     5281 cgggggcgat gaattcactg ttgcattcga cctttgaagc cttgctctat ggccgcgtcg
     5341 tcccagagga tcgcgcggtc tatccacccg gtgcgctggc caccatcgcc gccggagcat
     5401 tggaacggtt cggccagcgc aaggcgggag gcgccgatat ccacctgacc gatgtcgacg
     5461 ggccggacgg cgcaagaatc acagtgatcg aggccatcaa tgacgacaaa ccgtttctgg
     5521 tcgcctcgac ccttgccgaa ctcaacgagc gcggtctcga tgtgcgcctg gtggcgcatc
     5581 ccgtcctggc cgtccggcgc aacgacaagg gtgagctgac cagcctgggc gggcttgcag
     5641 gcgccgggcc ggacccggat tttgtgcgcg agagttttgt tcacatccac gttgcccgca
     5701 tcgtcggcaa ggccgcacgc gaagccctgg tccgctcact gcaggagatc tatcgcgaca
     5761 tcggcctggc cgtgcacgac tggaaggcaa tgcgcgagcg tctggcaggc gtggcccatc
     5821 gctatcgcga acatccgccg atgctgccgg ccgatgagat tgccgaggca gtgcagtttc
     5881 tcgactggct tgccgccgac aatttctcgt tcctcggaat gagggaatac cgttttgcca
     5941 gtctggcctc cgaaccggtg gccggagccg gcctcggcat cctgtccgat cctgaggtga
     6001 aggtactccg gcgtggcagt gaactggtgg taacaacgcc tgagatccgc gccttcctga
     6061 gcgagccgtc cgcgctcatc atcaccaagg ccaacgtccg ctcccgcatc catcgccgcg
     6121 ttcacatgga ctatatcggc gtcaagctga tcgacgagac cggcgcgctc gaaggcgaac
     6181 tgcgcctcat cggtcttttc acctcgacgg cctataccgg ctcgacgcgc accattccct
     6241 atattcgcca caaggtggcc caggtgatga tgaaagccgg gttcgatccg gcctcgcatt
     6301 ctggcaaggc gttgatgaat gtgctggaga gctatccgcg cgacgaattg ttccagctcg
     6361 accgggatac gctctatggc tttgccatgg atgcgatgaa cctcaacgag cgcccgcgca
     6421 tccgcgctct cgtccgcgcc gatcgtttcg accgtttcgt ttcggtgatc gtctatgtcc
     6481 ccaaggaccg ctatgatacc ggggttcgcc tgcgtatcgg cgatttcctg gcgcgcagct
     6541 tcgatggccg ggtctcagcc gcctacccgt cctatccgga agggccgctg tcacgcaccc
     6601 atttcatcat tggtcgccac ggcggcgcca cgccgaaggt cgaaaggtca accattgagg
     6661 caggcattgc cgccgtcgtc cggacatggg gcgatacgct caagaccacc atcgagacaa
     6721 cccatgcgcc ggacgtcgcc gagacgctgc tcgcccgcta tggctctgcg ttcagtgccg
     6781 gctatcgcga ggcttttgcg cccgcagagg cccttgcgga catcgccatc cttgaaaacc
     6841 tcagtgccga gcagccgacg gctgttgacc tttaccgggc gccacaggcg gaggcaggca
     6901 ctgcacggct caaggtctat gccttcggcg cgccgattgc cctgtcccgc cgcgtccctg
     6961 tcctggagaa cctcgggttt tccgtctcca acgagcgcac ctatgatctt cacctgccga
     7021 tgacggcagg caaggctgct gcctatctgc acgacatgac catcgcgcgg gcccgcaatg
     7081 gcaatatcga tgtcgatgtg atggaagagc gtgtcgaggc ggcgctgatg gccgtttttg
     7141 ccgatcagac cgaatccgac cgcttcaaca ccctcgttgt cgaagccggc ctgacctggc
     7201 gcgaagccat ggtgttgcgc gcctatggcc gctatctcgg tcaggccggt gccccctttg
     7261 cccagaatta cctcgccgat gtcctggcca aatatcccga gattgcccgc gggctgatcg
     7321 aactgttcgg cgcccgcttc gaccctgagc gccccggcga caataccgcc cgcatcgctg
     7381 ccggcaccgc tgtcgccgat cggctgcgcg ctgccatgga cgcggtgtcc aacctcgacg
     7441 aggatcgtat cctgcgccgc ttcctcaacc tgatcgaggc aacgctgcgc accaactact
     7501 accagcggct gggcagtagc gaaatgcgtc ctgcgctggc tttcaagttc gatccgcgcc
     7561 gcattgacgg cctgccgctg ccgcgcccgc agttcgagat ttatgtgcat tccccgcgtg
     7621 ttgacggcgt gcatatgcgc tttggcaagg tcgcgcgcgg cggtttgcgc tggtctgacc
     7681 ggccgatgga tttccgcacc gagatcctcg ggctggtcaa agcccagcat gtcaagaact
     7741 cggtcatcgt tccggtcggt gctaagggtg gttttgtccc caaacggctg cccccgccgt
     7801 ctgaccggga agcctggatg gccgaaggga tcgctgccta caagacctac atcaacgccc
     7861 tgctcgacgt gaccgacacg atctccggca ggggcatcaa ggggccggcc ggtgtcgtaa
     7921 ggctcgacgg cgacgacccc tatcttgttg tggccgcaga caagggcacc gcaaccttct
     7981 ccgacattgc caatgcgatt tccatcgaac gcggccactg gcttggcgac gcttttgcct
     8041 ccggcggctc gcaaggctac gaccacaaga agatgggcat caccgcgcgc ggcgcgtggg
     8101 aatgcgtcaa gcggcacttc cgcgaaatgg atatcgacat tcaggctcaa ccggtcaccg
     8161 tggccggcgt cggcgacatg tccggcgatg tgttcggcaa cggcatgttg ctgtcgaagg
     8221 ccctgcgact ggtcgcggcc ttcgaccacc gcgacatctt tctcgacccc gatcccgatc
     8281 cggcccgcgc ccatgccgag cgccagcggc tgttcgatct gccgcggtcg agctggcagg
     8341 attatgacag ggccctgatt tccaagggcg gcggcatcta ttcccgctcc gccaaggaaa
     8401 tcgtgctctc gcccgagctc cgcgcgaccc tcaagatcga ggcagagcgc gtcaccccgc
     8461 aggaattgat gatggcgatc ctcaaggcgc cggtcgatct tctgtggttt ggcggcatcg
     8521 gaacctatat ccgctccggt gaggaggccg acgtcggtgt cggcgatcgc gccaatgatg
     8581 cgatccgcat cccggcgacg gccctgaatt gccgggtgat tggtgagggc gccaatctcg
     8641 gcgtcaccca gcgcggccgt atagaagcct cccggcgcgg tatccgcctc aacaccgatg
     8701 ccatcgacaa ttccgctggc gtcaatacct ctgacgtcga ggtcaacctc aagatcgcgc
     8761 tggccatgcc ggttgccgac ggctcgctcg gtgaaaccga acgcaacagc gcgctcgccg
     8821 gaatgaccga cgaggtggcg gcgcttgtgc tgcgcaacaa ttatctgcaa tcgctggcgc
     8881 tgtcgctggc cgagcgccgt ggtgcggccg gcttggcaga acttcaacgg ctgatgcagg
     8941 ggctggaatc gcgagcgcgg ctggatcgtg cggtggaact actcccggcg gacaccgaga
     9001 ttgcccgccg catcgctgct ggcgagggcg ccacgcggcc ggagctggcc gttttgctgg
     9061 cctatgccaa gctgtcgttc ttcgacgatc tgatcgtctc cgaagtgccg tacgatccgt
     9121 attttgccga cgaactcgac cggtatttcc cgctggcggt gcgcggccgc ttcccggaag
     9181 cggtgaagaa tcatcggttg cggcgtgaga tcatcgccac gcaactcgcc aatatcgtcg
     9241 tcaatcgctg cggccccggt ggcatcgccc gtctgcagga cgagaccgga gccgacattc
     9301 cgtccatcgc ggccgcctat gcgctgaccc gcgatgcttt cggtctgctt gatctcaatg
     9361 gcgccatcga ccagctcgac acaaaaatcc ccggcgcggt gcaactcgat ctctatgcgg
     9421 cgttgcagga catgctggtc ggccgccttg cctgggtgtt gcgcaatgtc gatctgaccc
     9481 gcccgctcgg ccccgaggtg cgccgtttca aggccggcat cgacgcctcg cgcgcgcttg
     9541 ccccgcagtt gatcacgccg gcgcagaacc agcgccgctc cgagcgcctt gcggaatgga
     9601 ccggcaaggg tgtgccgcag acgctggcca tgagcatcgt cgatctgccg gcgctggtga
     9661 ctgcgcccga cgccgtcctg gtcgcggaac agacgggcaa gccggtccgc gacgtgctgg
     9721 aaacgttgtt tgcgctggcc gatcgcttca acatcggccg catccgtcag gctgcggcga
     9781 ctattccggc ccgcgaccgc ttcgagcggc tggccatcga ccgcgcgatt gatggcatgg
     9841 atctgtcctt gcgccgcctg gctggcgagg tgatcgccgg ctacggcccc ggcatggccg
     9901 gggtcgcgac gtttgccgcc aagcggggcc gcgccgtcga gcgtattacc gctgccattg
     9961 aagagatcgt tgccagcggc ctgacacagg ccaaattgac cattgccgcc aatctgctgg
    10021 gagacctggt ccgaagctga tgcaaagagc gcatcaatgc cggaaatgac ggatacccgt
    10081 catcaccatg gcaaggccag cctcgtcggc ggcggcgatc acctcgttgt cgcgcattga
    10141 gccacccggc tggatcaccg ccgtcgcccc ggccgcagca gcggcaagca ggccatcggc
    10201 aaacgggaag aaggcatcgg aggcgacgac cgaacccttg gccagcgatt ccgaaagccc
    10261 ggccgccttg gccgcttcat ccgccttcca ggcggcgatt cgcgccgaat cgacccggct
    10321 catctgcccg gcgccgacgc cgaccgtcgc cccgtccctc gcatagacga tggcgttcga
    10381 cttgacatgc ttggcaacac ggaaggcgaa cttcagatcc atgagttcgc gttcgctcgg
    10441 cgcgcgcctg gtgacaacct tgagattcat gtcatcgaca acggcattgt cgcgatcctg
    10501 caacaggaac ccgccggcca cgctgcggat catccgccct tcggcgcgcg gatcgggaag
    10561 gcccccggtc agcaacagcc gcaggttctt ttttgccttg atcagtgcga tcgcctcgtc
    10621 gctggcatcg ggcgcgatga tgacctcggt gaagatctcg acgattgacc gtgccgcgtc
    10681 ggcatccagc gtgcggttga gcgcaacgat cccgccgaag gcagagaccg gatcgcagcg
    10741 cagcgcccgc tggtaggcgt cgagcagcga agcgccttcc gccacaccgc acgggttcat
    10801 gtgtttgatg atcgccaccg cagccgtgcg ccgggggtcg aattccgcaa ccagttcaaa
    10861 ggccgcatcg gtatcgttga tgttgttgaa tgacagcgcc ttgccctgca cctgccgcgc
    10921 ggtggcgacg ccaaagcggc tttcaccggc gcggtagaag cccgccgcca tatgcggatt
    10981 ttcgccatag cgcatgcgct cgaccagccg gccgccgata gccgcatagg tcggggtccg
    11041 atcttcgagt tcggcagcga accaggtgga gatcgccgca tcataggccg ccgtgcgtgc
    11101 ataggccttg gccgcaagca tctggcggaa ggcaaaggag gtcgccccgt tctgggcgtc
    11161 cagttcagcg atgagagcgg caaaatccgc gacgtcgacg atgaccgtca catcctggtg
    11221 gttcttggcc gcagcacgga tcatcgccgg tccgccgata tcgatattct cgatgcaatc
    11281 ggcataggcc ttgccggccg caaccgtcgc ttcgaacgga tagagattga cgatcaaaag
    11341 atcgatcggc ggaatgtcgt gggcaagcag cgcggcttcg tgttcgggat gcccgcgctg
    11401 ggcgagcagg ccaccatgca ccttgggatg cagggtcttg acccgcccgt ccatcatttc
    11461 cggaaacccg gtaatgtcag caacttcggt gacggcacag ccggccgcct tgagcgcctg
    11521 ataggtgccg ccggtggaaa caagcgtcac gccgcgctgg ccgagcgctt ggccaagttc
    11581 ggccaggccg gtcttgtcgg agacggaaat gagggctcga cggagggggc gcagggtctg
    11641 gggcatcgga actgggccgg gctggtttct gaggatggcc ggccctagca cttaaatcca
    11701 tgcaacgcca tcagaccaac cgctagatta cgaagaacca agcgccgcct ggacaaggct
    11761 agctcatcac caaccgcccg cccgatcgcc gggcaaaact ccagaccact tcaggctcat
    11821 tgccgtcgag ggcaacggtg atctgctggc agcgacgctg cgaggccagc ccgccgaaaa
    11881 agatgctgtc ttcgagggcg agactgcgac cgccggcact gaacgtccag ctttcgccgc
    11941 ttgcgagttg aatttccacc gcacgaccat ccccggaaag gaaagcccgg acctgcggat
    12001 gcagatggaa acgaagcctt gccgtggccc gcaccccacc tccggccgac aggccaataa
    12061 ggctatcgcg cccggacaga tagctgcctt cagccgtcag ggtcagttgc cgccgatgga
    12121 ccagtccgtg cgtggcttca tagcagcgat ccgtcatatc gacactgtcg ccgtccgctt
    12181 ccacccgtcg ttccaccgcc ggcgcccgct ggcgtgcgct cgcgcggcgt tcagcttccc
    12241 cgtcggcggt caacgcatcc gaaaagctca gccgcgaaat cccctccaga tcaagcgtcg
    12301 aatgggcggc cgtctcccgc aaggcctcac gcattcccgc cagccccgcc ggagccgccc
    12361 cgcaattgac gacgatacgc tcatggccgg aggacagttc aaacgatgtt gcaccggcat
    12421 ggaagcgggc cgtcattccg ctcggcgggc acggaccggc atcgaccagc accacggagc
    12481 ggccaacggc cagccggtga tatccgcaca tgcccgtcac cgggaaggac tcggacgtgg
    12541 aatcgagaaa ctgtatcaga gaaccgatca cagtgagctg gcttgcgctc atcccgttga
    12601 acagcgcgat gtcgttgccg ggatgacgca ggaacatcag aaatctcagc agcccgtcga
    12661 tggtcgtttc cagaacgggt ggcggcctgt tgttccgggc tgaaaaggcg gtgcgcagcg
    12721 gcaacagcat cgccagcagg acaagcgccg aattcggatt gcggtcgacg gggccgccgt
    12781 cgccggcctg aaccatcgac aagctgcgtt cgagcaaccg cacggcccgc ttgagtgtcc
    12841 gttccggggt cagcgcgcaa agcgcataaa gaccaaaggc aatggcggct tcagcgcggg
    12901 ctatccaatc gcgatcccgc tcggccgccc gctccagcgc tttggcctgc ccggccagcg
    12961 cccgcatgaa ccggacatag aaatcgtgat cggcgccggt cagcagcagc ggcgcattga
    13021 ccagaaatgc ggtcatgcga tgagcgagca catcactgcg ctgggctgcc ggtcgctggg
    13081 ctagggtcgg gttgagcaac cagtcgtcga ccagcgcgcg cgcatgcaca cggatcaccg
    13141 gttcttccga ttcgttgaaa tgccgcagcc aggcaaaact gtgcaattcc cgcagccagg
    13201 caggatgcgg cgcctccacc gaaaacggtg actggccgcg tgtcagcacg gttcgaccgg
    13261 ccagcgtata gctgccggcg tagatgtcgc gggccacggt aggatcgccc gtttcgatct
    13321 cttgcggtgc gatcagcagc cgttccggca ctttcagccg gaaattgcgc cagagcgtgc
    13381 cgaggccgaa tcgcccgcgc atgccaccgg acgccggagg acgcggcgtg cccagtccga
    13441 tggtcaaagc cccgtcctct ttgtcattgc acccccttgg caaccccttg ctgccgcccg
    13501 tggccggagt tgagttgatg gaatgggcac tcgtcaaggc cgctgcttcc gcaaccgcgc
    13561 cgaaaaaaat ccgtcgcagc cgccaaaagt gccaagatgg cagggcaagg cgcgcatcaa
    13621 tccgcctgct gtgatcattt ctgcacaatc accagcctct gctgcgccga tcggatcaag
    13681 ctcggcatcg ctgtggtcgg cgaggaatcg ggcgatctgc tcctcgccct cttccggttc
    13741 gagcgaacag gtcgcataga ccagcacgcc tccgggctcc agcagcgtcc aggcatgatc
    13801 gacaagccgg cgctgcacag cgatcagcgc ggcaagatca gccagtgtct tgttccaggc
    13861 aacatcggga tggcgccgga tggtcccggt cgccgaacac ggagcatcta ggaggatgcg
    13921 ctgaaaacct ccctgtgaaa acgccccgcc atcggcggca tgcacttcgg ccgcaagacc
    13981 aagccgcgcc agattggctt ccaggcgctt gagccgctgc ggcgaacgat caacggcaac
    14041 gacccgcgca cccatggcag ccagttgggc ggtcttgcca cccggcgccg cacacagatc
    14101 aagaacgcgc tggcccggtt caaccctgag caaccgtgcc ggcagcgctg ccactgcatc
    14161 ctgcacccac catgcccctt cggaaaatcc gggcagatcc ggcacgcgtt gcccgctgtt
    14221 gaggcgcagg ctgccggttg gcagccgcac tccgcccagc ctctcggccc aggtgatggc
    14281 gtcggcccgc acagtaaggt cgatgggcgg gggcaccaga tgcgcggccg cgatcgaacg
    14341 ggcgacgtct tcgccgtaat gccggcgcca gcgtgcggcc agccactccg gggtattgtg
    14401 ggtcagcgga tcgagtcggg ccagcagcgc cgccttgtcg cgcgccacgc cgcgcagcac
    14461 ggcattggcc agtccggcat agcgctggcc gatccgatcg gccctgagct tggcgaccgt
    14521 ggtatccacg gcggcataat cgggtacgtc aagaaacaat agctgcgcca gccccgccag
    14581 cagcagcggc tgcaacgggc cggactgcgg cggcaggccg tccttgagtc gctcgtcgag
    14641 acaatgctgg agatagccga gccccctgat cgtcgccacg gcgatggcgc gtgccaaccc
    14701 gcgatcggcc ggagcaagcg cctcgaacac cggatccttg ctgaaccggt caagcacatc
    14761 gtcgagtggc aggcgtcggc gcacgacatc gctgatcgcg cgcacggcaa aatcgcgggc
    14821 atccaccgca acggattgac gcgcctccgg ccgggtcatg cttttggttc cacaaggatc
    14881 gcatccggat caagcccgat caggccgacc agcgcccgca tatggtcagg cagcggcgcc
    14941 gtcacagtga ccgcagccac gccctcacgc gagggcagaa cgatctgtcg cgcgtgcagt
    15001 tgcagctgcc gggccagcgc ccgcgcccga tctcctccgt agagaacatc gccggcgatc
    15061 ggacagccca gggctgcgct gtgcacgcgg atctgatggg tgcggccggt ctgcggctcg
    15121 aactgaacca ggctgaggcc atcgccggcc gccagaacgc gatagcgcgt gatggccgcc
    15181 tttccctccc gatctccggg atcggcaatg cgcatca
//
LOCUS       DAOMXG010000044        15180 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_2171_length_15180_cov_10.932298, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000044 DAOMXG010000000
VERSION     DAOMXG010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 15180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15180
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2171_length_15180_cov_10.932298"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1601)
                     /gene="ade"
                     /locus_tag="PLQ11_04695"
     CDS             complement(<1..1601)
                     /gene="ade"
                     /locus_tag="PLQ11_04695"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HRK24234.1"
                     /translation="MDRATLARRIDQGRGLEPADLVIKNGRFLDLVSGDLVASDIAIC
                     GQTIVGTHGAYEGRSVIDAAGRIIVPGFVDTHTHVESSLLTPQEFDSCVLPYGVTTVI
                     CDPHEIANVIGAEGIRYFTASAETLVMDLRINLSSCVPATHLETAGARLDAADLVALR
                     NHPKVIGLAEFMNFPGVLFKDAGCLDKLAAFAGGHIDGHAPLLRGRDLNGYLAAGIRT
                     DHECTTAAEAREKLSKGMNILIREGSVSKDLHALAEILTERTAPFCGFCTDDRNPLDV
                     AEEGHLDFIIRTAIALGVPPLAAYRAASWSGARIFGFNDRGLIAPGWRADLALIDALE
                     SCRVSDVVAAGRLVGPDLFAARRPVEPVGRHSMKARAVAAADFRHPARQGATPVIGVI
                     PGKIITDRLTLALPIRDGATGCDLDQDVIKVTVIERHGRSAPGEGIGRGFVKGFGMKR
                     GAIASSVGHDSHNICVVGADDADIAVAANRLGEIEGGFVVVAGGQVLAELALPVAGLM
                     SLAGRTVVHDRLVALRGAARSLGVTL"
     gene            1666..2625
                     /locus_tag="PLQ11_04700"
     CDS             1666..2625
                     /locus_tag="PLQ11_04700"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase A"
                     /protein_id="HRK24235.1"
                     /translation="MSKAKPPLILSYTRWDEAPWLARFRAAAPDRPVLSLAEVKDTLP
                     DRFYLSCWRPPADLFRIIPKPLLILCPGAGVDNVMAAAPPPDVPLTRIVDPDLTGRMV
                     EYVALHALMHLRQMTNYQTSQQERRWRPRHQPAAREVTVGILGAGEMGLAAATALKAV
                     GFQVIGWRRSPAPDAPIPIHAGAAGLPAFLAATDILVNLLPSTPETRGLIDAALIGCL
                     RNPGPLGAPAFINAGRGDTVQDADLIAALEDGRLRAASLDVFTVEPLPVDSPYWDLPN
                     VIVTPHVAADSMPDAVCAAILAEISAFEAGRPLIHPVDRSKGY"
     gene            2632..3057
                     /gene="arfB"
                     /locus_tag="PLQ11_04705"
     CDS             2632..3057
                     /gene="arfB"
                     /locus_tag="PLQ11_04705"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HRK24236.1"
                     /translation="MLEISKDIVIDDSDIEISFVRASGPGGQNVNKVATAVQLRFDVR
                     RSRGLPDAVAVRLMTLAGRRLTKDGVLVLTADRFRTQEMNRQDALDRLAGLIREAAAP
                     PPPKRRATRPTKASVERRLTTKKGRGGVKRARSDKRFDE"
     gene            complement(3086..5518)
                     /gene="gyrB"
                     /locus_tag="PLQ11_04710"
     CDS             complement(3086..5518)
                     /gene="gyrB"
                     /locus_tag="PLQ11_04710"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HRK24237.1"
                     /translation="MAETPRDTSNDNDYGADSIKVLKGLDAVRKRPGMYIGDTDDGSG
                     LHHMVYEVVDNAIDEALAGHADIVTVTLNPDGSCTVTDNGRGIPTDIHPGEGVSAAEV
                     IMTQLHAGGKFDQNSYKVSGGLHGVGVSVVNALSKWLKLKIYRNDQSHEMEFSHGDAV
                     APLKVTGQAIGKKGTEVTFLPSLETFKGVTEFDYGTLEHRLRELAFLNSGVRIVLTDR
                     RHADVKREEMLYEGGLEAFVRHLDRTKTALIQKPVMIRRDQDGITVEAALWWNDSYHE
                     NVLCFTNNIPQRDGGTHLAGFRGALTRQVTGYAESSGISKKEKVSLTGDDCREGLTCV
                     LSVKVPDPKFSSQTKDKLVSSEVRPVVESVCNEALSIWLEENPAEARTVVGKVVEAAA
                     AREAARKARELTRRKGALDIASLPGKLADCQERDPSKCELLLVEGDSAGGSAKQGRDR
                     LYQAVLPLRGKILNVERARFDKMLSSQEIGTLITALGTGIGREEFNVDKLRYHKIIIM
                     TDADVDGSHIRTLLLTFFFRQMPEVIERGHLYIAQPPLFKATRSKSEQYLKDERALEE
                     YLISGGIEGAVLKLANGEERAGPDLRRSIDEARLIRQIMSGLHSRYDRGIIEQAAIAG
                     ALRPVNEDETSAMQALATVIAQRLDILADPLERGWQGDVRHDSLVFSRMVRGVKQAAV
                     LDASLLASAEARRLNERSSSLSESYGAPAMLIRKGDERPISGPLALFDAITGTGRKGV
                     TLQRYKGLGEMTAQQLWETTLDRNVRSLLQVKIKEVDAADNVFVKLMGDVVEPRREFI
                     QDNALNVANLDI"
     gene            complement(5597..6211)
                     /locus_tag="PLQ11_04715"
     CDS             complement(5597..6211)
                     /locus_tag="PLQ11_04715"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HRK24238.1"
                     /translation="MTLSGFALYISVYFAAVASPGPGIALVLARAMGKGLDRVGWFVA
                     GFALGDTVLFVLAASGLAIIAQTFEDVFLIIRYLGAAYLIYLAVKIWRAPATAIDITA
                     ETLRETPVQAFLSSFLLTLGNPKPIMFFLSIMPLVVDLKSLTAETFLILGLANIVVIV
                     PVMLAYALLADRTRRLFRSPEAVRRINRTTAGVMAGTALVMATR"
     gene            complement(6208..7344)
                     /gene="recF"
                     /locus_tag="PLQ11_04720"
     CDS             complement(6208..7344)
                     /gene="recF"
                     /locus_tag="PLQ11_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HRK24239.1"
                     /translation="MPIACRIRRLHLSDFRTYTASEMEVGSALVALVGDNGAGKTNVL
                     EALSLLAQGRGLRRADLADMARRSGDGSFAVAVEAEGGLGPCHLGTGVIAGETGRRNR
                     INREPASSPIAFADHLRIVWLTPDQDGLFRAAAGERRRFLDRLVLAVDPAHAARVSAL
                     ERALRARNRLLEEFAPDRHWLDAAEHEVAEIAVAVAAARRETVDRLAHLIATGRDEAS
                     VFPYPVLTLEGALEERIAAEPASAVEDWYRAELAAGRARDKAAGRTLTGPQASDLAVR
                     HGPKDMPARLCSTGEQKALLIALVLAHARLVTAMTGMAPITLLDEVAAHLDPGRRRAL
                     IGALGALGGQVWLTGADPSLTDDLHASADCFAVTPGHIMPIVAS"
     gene            complement(7394..8512)
                     /gene="dnaN"
                     /locus_tag="PLQ11_04725"
     CDS             complement(7394..8512)
                     /gene="dnaN"
                     /locus_tag="PLQ11_04725"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HRK24240.1"
                     /translation="MKVTIEQAALLKALGHVHRVVERRNTIPILSNVLIRAEERRLQL
                     KATDLDIEVTEVIEADVSQAGATTVPAHVLHDIVRKLPDGAQVSLEQTGDTGQLLLRS
                     GRSRFVLQSLPAGDFPDLAAGVLSHAFDVPATELRRLIEKTQFAISTEETRYYLNGIY
                     LHCVAGEAGAVLRAVATDGHRLAQADTPAPAGASGMPGVIVPKKTVAEIQKLLAEASG
                     EVKVELSSAKVRFTTGSVVFTSKLIDGTFPDYARVIPTRNDKRLTVELAEFAGSVDRV
                     STISSERGRAVKLALADGKLTLSVNNPDAGQATEELSVDYGETPLEIGFNARYLLDIT
                     QQLDGDTALFKLAEPGSPTLIQDREGAPTLYVLMPMRV"
     gene            complement(8668..10155)
                     /gene="dnaA"
                     /locus_tag="PLQ11_04730"
     CDS             complement(8668..10155)
                     /gene="dnaA"
                     /locus_tag="PLQ11_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HRK24241.1"
                     /translation="MFAEKTPVALPAAAGASEVEPHGADTVWGRVRARLRAELGEDIF
                     TSWFARLDLVSIADGVAHLSVPTRFLRSWLHGHYIDRIEAMFAEERDDVRKVSIALRE
                     AGQKVVLARISPASPPAGGPSSAPAAADVVVAATPPGATPTRAQDYFESSPLDRRLVF
                     STFHIGRSNILAHSAALQVVQAAPADMPVYNPLFVHSAVGLGKTHLLQATAQAVAANG
                     RRVIYLTAEKFMYGFVAALRSQTALAFKETLRAIDLLVIDDAQFLNGKQVQQEFCHTL
                     NALIDAGKQVIVAADRSPADLESLDERIQSRLAGGLVVEIGALDEALRLKVLESRIQT
                     ARMRLPGFAIAPDVIAHVAQLVTANGRDLEGAVNRLLAHATLSGGPLDKEGAELAIRD
                     LIRTHEPKKVKIEDIQRLVANHYNVSRADILSSRRTATVVRPRQIAMYLAKLLTLRSL
                     PEIGRRFGGRDHTTVLHAVRKIDGLVARDTSLAGEIDHLKRMLQE"
     gene            complement(11266..11532)
                     /gene="rpsT"
                     /locus_tag="PLQ11_04735"
     CDS             complement(11266..11532)
                     /gene="rpsT"
                     /locus_tag="PLQ11_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HRK24242.1"
                     /translation="MANTKSAKKAARQALRKTEVNKARRSRTRTSIRKVEEAIASGNA
                     EAAATALRAAEPEMMRAARSGVMHKNTASRKVSRLTKAIKAIKA"
     gene            complement(11711..12517)
                     /locus_tag="PLQ11_04740"
     CDS             complement(11711..12517)
                     /locus_tag="PLQ11_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HRK24243.1"
                     /translation="MIETLAELYGLPLASVAGLVAVSFTAAMIDAVAGGGGLLNVPAL
                     MLAGLNPVAAVATNKLQGTAGAISATRSYMRAGLIDRVAMRGAFLASLAGGAMGAVSA
                     QIVPIDALRTGVPVLLIGVALFVAASPKLSDVASRARLGLMPYALGVALVIGFYDGIF
                     GPGGGTFYFISLVLLLGQGVTRAAGNAKFLNLASNAGALGLFAFSGQVWWALGLTLGL
                     ASMLGARIGARAALKRGAGLIKPLVVIVSILMALRLLADPTHPIGQWLRS"
     gene            complement(12557..13342)
                     /locus_tag="PLQ11_04745"
     CDS             complement(12557..13342)
                     /locus_tag="PLQ11_04745"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HRK24244.1"
                     /translation="MGLKPSYDNIIAETHGRVGLIRLNRPQALNALNSALIADLNHAL
                     DVFEADEAIGCLVLTGSEKAFAAGADIKEMQSKTYMDVYLSDFIGRWDNLSRTRKPVI
                     AAVAGFALGGGCEIAMMCDFIIAAETAKFGQPEIKLGVMPGAGGTQRLTRYIGKSKAM
                     DLCLTGRMMDAAEAERCGLVSRIVPADQLVSEAMKAAETIATMSLPAVMMTKESVNRS
                     YETTLAEGIRFERRVFHSMFATDDQKEGMAAFAEKRKASFRHR"
     gene            complement(13446..14327)
                     /gene="mutM"
                     /locus_tag="PLQ11_04750"
     CDS             complement(13446..14327)
                     /gene="mutM"
                     /locus_tag="PLQ11_04750"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015319341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HRK24245.1"
                     /translation="MPELPEVETVRRGLAPVLEGQRLASVRINRPNLRFPFVPRFVER
                     LTGQRVDRLNRRAKYLIADLGSGEHLILHLGMSGRFVVEQIDETRQPGAFHQAIPRLA
                     AHDHVVFETAAGARIVYNDPRRFGFMVLVEAGALCDHPLLAGLGIEPLSNDFHAGAID
                     RLFAGKAAPLKAALLDQRLIAGLGNIYVCEALHRAGLSPARPAGTLSGARRRAACARE
                     RLAGAIRDVLTEAVAAGGSSISDHARTDGSLGYFQHAFRVYDREGAPCPTPGCHGHIQ
                     RQVQSGRSTFSCIRCQK"
     gene            14426..>15180
                     /gene="ubiE"
                     /locus_tag="PLQ11_04755"
     CDS             14426..>15180
                     /gene="ubiE"
                     /locus_tag="PLQ11_04755"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HRK24246.1"
                     /translation="MARAARPSAARVATSPREDESTHFGFQDVPLGEKQGRVNEVFHS
                     VAKKYDLMNDLMSAGLHRAWKDAMISMLRPPRGEGPYRHLDVAGGTGDVAFRILKAGG
                     AGMAVTVLDINGDMLAVGRDRAERQDFAGAIDFVEANAEALPYPDRSFDSYTIAFGIR
                     NVPRIDVALREAFRVLKRGGRFLCLEFSPVDMPVLDRIYDAYSFNVIPLVGQAVTGDA
                     DSYRYLVESIRKFPRPVLFADMIRAAGFGRVTMR"
BASE COUNT         2829 a         4855 c         4791 g         2705 t
ORIGIN      
        1 agcgtgacgc cgagactgcg ggcggcgccg cgcagcgcga ccagccggtc atgcacgacc
       61 gtccggccgg caagactcat caggccggcg accggcaggg caagctcggc cagcacctgg
      121 ccgccggcca caaccacaaa tccgccctcg atttcgccaa gccggttcgc ggccacggct
      181 atatcggcat cgtcggcgcc aaccacacag atattgtggc tgtcatggcc gacggacgag
      241 gcaatggcgc ctcgtttcat gccaaaacct ttgacaaatc cgcgcccgat gccttcgccg
      301 ggcgccgacc ggccatgccg ctcaatgacg gtgaccttga tcacgtcctg atccagatcg
      361 catccggtcg ccccatcgcg gatcggcaag gcaagtgtga gacggtcggt gatgatcttg
      421 cccgggatga caccgatgac gggtgtggcc ccctggcgcg caggatgacg gaaatcggcc
      481 gcggcgacgg cgcgtgcctt catcgaatgg cgaccgaccg gctcgaccgg ccggcgggcg
      541 gcgaacaggt ccggccctac cagccggcca gcagcgacaa catcggagac gcggcagctc
      601 tccagcgcgt cgatcaacgc cagatcggcc cgccatcccg gcgcgatcag cccgcgatcg
      661 ttgaagccaa agatccgcgc acctgaccag gaggcggccc ggtaggcggc aagcggcggc
      721 acgcccagtg cgattgcggt gcggatgatg aaatcgaggt ggccttcctc ggcgacatcg
      781 agtggattgc ggtcatccgt gcagaagccg cagaacgggg cggttcgctc ggtcaggatc
      841 tcggccagcg catgcaggtc tttggagact gatccttcgc ggatcaggat gttcattccc
      901 ttggacagct tctcgcgcgc ctccgccgca gtcgtgcatt cgtggtcggt gcggatgccg
      961 gccgccagat agccgttcag gtccctgccc ctgagcaggg gcgcatggcc gtcgatatgc
     1021 ccgccggcaa aggccgccag cttgtcaagg cagcccgcat ccttgaacag gacgccggga
     1081 aagttcatga attcggccag gccgatgacc ttgggatggt tgcgcaacgc gacgagatcg
     1141 gcggcgtcga gccgggcgcc tgcggtttcc agatgtgttg ccggcacgca ggacgaaagg
     1201 ttgattcgca ggtccatcac cagggtctcg gcgctggcgg taaagtagcg gatcccctct
     1261 gccccgatca cattggcaat ttcgtgcgga tcgcagatca cggtggtgac accgtagggc
     1321 aggacgcagg aatcgaactc ctgcggggtc agcagcgagg attcgacatg cgtgtgcgta
     1381 tcgacaaaac cgggcacaat gatccgaccg gcggcatcga tcacgctccg cccctcgtag
     1441 gcgccatgcg tgccgacaat ggtttggcca cagatcgcaa tgtcggaagc aaccagatcg
     1501 ccgctcacca gatcgagaaa gcggccgttc ttgatgacca gatcggcagg ttccagtccg
     1561 cgtccctgat cgatgcggcg ggcaagtgtg gcgcgatcca tcgattgtct ccctcagggg
     1621 ttcgacgtta ccatgacagc aatgccgctg cgagccggag tcatcatgtc caaggcaaag
     1681 ccaccgttga tcctgtccta tacccgctgg gacgaggcgc cttggctggc ccgcttccgc
     1741 gcggccgcgc cggaccgccc ggtcctgtcg ctggccgagg tgaaggacac cctgcctgac
     1801 agattctacc tgtcctgctg gcgcccgccg gctgatctct tccgcatcat tcccaagccg
     1861 ttgctgatcc tgtgccccgg tgcgggggtc gacaatgtta tggcggccgc cccgccgccc
     1921 gatgtgccgc tgacccgcat tgtcgatccg gacctgaccg gtcgcatggt cgaatacgtg
     1981 gccttgcatg cgctcatgca tctgcgccag atgaccaact accagaccag tcagcaggag
     2041 cggcgctggc gtccgcgcca ccagccggcg gcccgcgagg tcacggtggg cattctcgga
     2101 gcaggggaaa tgggtcttgc ggcggcaacg gcgctcaagg cggtcggctt tcaggtgatt
     2161 ggctggcggc gttcgccggc gccggatgcg ccgatcccga tccatgccgg cgccgcagga
     2221 ttgccggcat ttctggctgc gaccgacatt ctggtcaatc tcttgccttc aacccctgaa
     2281 acccgaggtt tgatcgacgc ggcactcatc ggctgcctca ggaaccccgg cccgctcggt
     2341 gcgcccgcct tcatcaatgc cggtcgcggc gacacggtgc aggacgccga tctgatcgcc
     2401 gcccttgagg acggccgact gcgcgccgcc tcgctcgatg tgttcacggt cgagccgctg
     2461 ccggtcgaca gcccgtactg ggatctgccg aacgtgatcg tcaccccgca tgttgccgcc
     2521 gattcgatgc ccgatgcggt ctgcgccgcc attctggccg agatttcggc tttcgaggcc
     2581 gggcggccgt tgatacatcc ggtcgatcgg tcaaagggct attgaaacgt catgcttgag
     2641 atcagcaagg atatcgtgat cgatgacagc gatatcgaga tcagcttcgt gcgcgcctcc
     2701 ggcccgggcg gtcagaatgt caacaaggtg gcaaccgccg tccagcttcg cttcgatgtg
     2761 cgccgttcgc gtggcttgcc ggatgccgtg gcggtgcggc tgatgacgct ggcgggccgc
     2821 cggttgacca aggacggcgt tctggtgctc accgccgacc ggttccgcac ccaggagatg
     2881 aaccggcagg atgctctcga ccgtcttgcc ggcctcatcc gcgaggcggc agccccgccc
     2941 ccgccgaagc gccgggcaac caggccgacc aaggcttcgg tggagcggcg gctgacgaca
     3001 aagaaagggc ggggcggcgt caagcgcgcc cgctcggaca aacgctttga cgaatgaacc
     3061 cggcacccgc agccgccgga cgcgatcaga tgtcgagatt ggcgacgttc agcgcattgt
     3121 cctggatgaa ctcgcgccgc ggctccacca catcgcccat cagcttgacg aaaacgttgt
     3181 cggcggcatc gacttccttg atcttcacct gcagcagcga acggacattg cgatccagcg
     3241 tcgtctccca caattgctgg gcggtcatct cgcccaggcc cttgtaacgc tgcagggtga
     3301 cccccttgcg cccggtgccg gtgatggcat cgaacagggc aagcgggccg gaaatcggcc
     3361 tttcatcccc cttgcggatc agcattgccg gcgcgccata gctctcggac agcgagctgg
     3421 agcgctcgtt gagccggcgt gcttcggcgg aggcaagcag cgacgcatcg aggacggctg
     3481 cctgtttcac accgcgcacc atgcgtgaaa agaccagcga atcgtgcctg acgtctccct
     3541 gccagccccg ttcgagcgga tcggccagaa tgtcgagccg ctgcgcgatc acggttgcca
     3601 gcgcctgcat tgcagatgtt tcgtcctcgt tgaccggacg cagcgcaccg gcgatcgccg
     3661 cctgctcgat gatcccccga tcataacggc tgtgcaggcc gctcatgatc tggcggatca
     3721 gccgcgcctc gtcgatcgaa cggcgcaggt ccggcccggc ccgttcctcg ccattggcca
     3781 gtttgagcac ggccccctcg atgccgccgg agatcaggta ttcctccagc gcccgttcgt
     3841 ccttgagata ctgttcggac ttggagcgcg ttgctttgaa cagcggcggc tgggcgatgt
     3901 agagatgtcc acgctcgatg acttccggca tctggcggaa gaagaacgtc agaagcaggg
     3961 tccggatgtg gcttccatcc acatccgcgt ccgtcatgat gatgatcttg tggtaacgca
     4021 gcttgtcgac gttgaattcc tcgcgcccga ttccggtgcc gagcgcggtg atcagcgtgc
     4081 cgatttcctg gcttgacagc atcttgtcga aacgcgcgcg ctcgacattg aggatcttgc
     4141 cgcgcagggg cagcacggcc tgatagagcc ggtcgcggcc ctgcttggcc gagccgccgg
     4201 ccgaatcgcc ctcgaccagc agcagctcgc atttcgacgg atcgcgctcc tgacaatccg
     4261 ccagcttgcc cggcagcgag gcgatgtcga gcgcgccctt gcgccgggtg agttctcgcg
     4321 cctttcgggc ggcctcgcgg gcagcggctg cctcgaccac cttgccaacc acggtcctgg
     4381 cttcggccgg attttcctcc agccatatgg acagcgcctc gttgcacacg ctttccacca
     4441 ctggacgcac ttcggacgaa accagcttgt ccttggtctg ggacgagaat ttcggatcag
     4501 gcaccttgac cgacagcacg caggtgagcc cctcgcggca atcatcgccg gtcagcgaga
     4561 ccttctcctt cttcgaaatg ccggagcttt cggcgtaacc ggtgacctgc cgggtcaatg
     4621 caccgcggaa gccggcgaga tgcgtgccgc catcgcgctg cggaatgttg ttggtgaagc
     4681 acagcacatt ctcgtggtag ctgtcgttcc accacagcgc cgcctcgacg gtgatgccat
     4741 cctgatcgcg gcggatcatc accggttttt ggatcagcgc cgttttggtg cgatcaagat
     4801 ggcggacgaa ggcctcaagc ccgccctcgt aaagcatttc ctcgcgcttg acgtcggcat
     4861 ggcgacgatc ggtcagaacg atgcgtacac cggaattgag aaaggcgagt tcgcgcagac
     4921 ggtgttcaag cgtgccgtag tcgaactcgg tgaccccctt gaaggtttcg aggctgggga
     4981 ggaaggtgac ctccgtgccc ttcttgccga ttgcctggcc ggtcaccttc aggggcgcta
     5041 cggcatcgcc atgcgaaaat tccatctcgt gcgactggtc gttgcggtag atcttcagtt
     5101 tcagccattt cgacagagca ttgaccaccg aaacgccgac gccgtgcaga ccgcctgaca
     5161 ccttgtagga attctggtcg aacttgcccc cggcgtgcag ctgggtcatg atcacttccg
     5221 ccgccgaaac cccttcgccg gggtgaatat cggtcggaat gccgcggcca ttgtcggtga
     5281 cggtgcagga accgtccgga ttgagggtca cggtcacgat atcggcatgg ccggcgagtg
     5341 cctcgtcgat cgcattgtcg accacctcgt agaccatatg gtgcaggccc gaaccgtcat
     5401 cggtatcgcc gatatacatg ccggggcgct tgcgcaccgc atcgaggccc ttgaggacct
     5461 tgatggaatc ggcgccgtag tcattgtcgt tcgaagtgtc gcgcggcgtc tcggccatca
     5521 gatatcagct ttcagtccat tgattcgaaa atggaatata gccgtttggc gcgtcagttt
     5581 cacggcgtca ttcacatcat cgtgtcgcca tcaccagcgc tgttccggcc atgacgccgg
     5641 cggtggtgcg attgatgcgg cgcacggcct ccggcgagcg gaagagccgc cgcgtccggt
     5701 cggccaacaa tgcataggca agcatcaccg gcacgatcac gacgatgttg gcaaggccga
     5761 ggatcaggaa ggtctccgcc gtcagcgact tgagatcgac gacgagcggc atgatcgaga
     5821 ggaagaacat gatcggcttg ggattgccga gggttaaaag gaagctcgac aggaaggcct
     5881 gcaccggcgt ctcgcgcaaa gtctctgccg tgatgtcgat ggcggtcgcc ggcgcgcgcc
     5941 agatcttgac ggcgaggtag atcagatagg ccgcgcccag ataacggatg atcaggaata
     6001 catcctcgaa cgtctgtgcg atgatcgcca gccccgaggc ggcgaggaca aacagcaccg
     6061 tatccccgag agcgaagccg gcgacgaacc agccgacacg atccaggccc ttgcccatgg
     6121 cgcgggcaag aaccagcgca attcccggtc cgggtgaggc gacggcggca aaatagaccg
     6181 atatgtaaag agcaaagccg gacagcgtca tgatgcaacg ataggcatga tgtgacccgg
     6241 tgtcacggca aaacaatcgg cactcgcgtg aagatcatcg gtcagcgaag gatcggcgcc
     6301 ggtcagccag acctgcccgc cgagcgcgcc gagcgcaccg atcagcgcac gccgccggcc
     6361 gggatcaaga tgggctgcaa cctcgtcgag cagggtgatc ggtgccatgc cggtcatggc
     6421 cgtgaccagc cgggcgtgag cgagaaccag cgcgatcagc agggccttct gctcgccggt
     6481 cgaacacagc ctcgccggca tatccttggg tccgtgccgc accgccagat cgctggcttg
     6541 cggcccggtg agcgtcctgc ccgcagcttt gtcgcgggcc cggcccgccg caagttcggc
     6601 gcgataccaa tcctcgacag cgctggcagg ctcggctgcg atgcgctcct ccagcgcccc
     6661 ttccaaggtc agaacggggt agggaaacac cgaagcctcg tcgcggcctg tggcaatcag
     6721 atgggcgagg cgatcaaccg tctcgcggcg tgcggcggca accgcgaccg cgatttcggc
     6781 gacctcgtgt tcagccgcat caagccagtg acgatcgggc gcaaactctt cgagaagccg
     6841 gttgcgcgcc ctgagcgccc gttcgagcgc actgaccctt gcggcgtgag caggatcgac
     6901 cgccagcacc agccgatcga gaaaccggcg ccgttccccg gctgcggcgc ggaacagccc
     6961 gtcctgatcg ggggtcaacc agacaatgcg caaatgatcg gcaaaggcga tgggggaact
     7021 ggccggctcg cgattgatgc ggttgcggcg ccctgtctct ccggcaatga ccccggtgcc
     7081 cagatgacag ggaccaagcc caccctccgc ctcgacagcc acggcaaagc tgccgtcgcc
     7141 gctgcgccgg gccatgtcgg ccagatcggc acggcgcagg ccgcggcctt gcgccagcaa
     7201 cgacagggct tcaagcacat tcgtcttgcc ggcgccgttg tcgccaacca gcgcaaccaa
     7261 tgcgctgccg acctccattt cggaggcggt gtaggtacgg aaatcggaca ggtgcagccg
     7321 gcggatgcgg caggctatcg gcatgcaatc aacctcggct tcccggcgga gccgtgcgac
     7381 ggcagggcgc ggctcacacc cgcatcggca tcagcacata cagcgtcggc gccccctcgc
     7441 gatcctggat cagcgtcggc gagcctggct cggccagctt gaacagcgcg gtgtcgccat
     7501 caagctgctg ggtgatgtcg agcagatagc gggcattgaa cccgatctca agcggggttt
     7561 cgccatagtc gacggacagt tcctcggtcg cctggccggc atcgggattg ttgaccgaca
     7621 aggtcagttt gccgtcggcc aatgccagtt tcaccgcccg cccgcgttcg gatgaaatcg
     7681 tcgagacgcg atcgacagag ccggcaaatt cggcaagctc caccgtcagg cgcttgtcgt
     7741 tgcgggtggg gatgacccgg gcataatcgg ggaatgtgcc gtcgatcagc ttggacgtaa
     7801 acacgaccga ccctgtggtg aagcggacct tggcgctcga cagttccacc ttcacttcgc
     7861 cgctggcctc ggccagaagt ttctggattt cggcaacggt ctttttcggc acgatgacgc
     7921 caggcatgcc ggaagctccg gccggtgccg gcgtatccgc ctgggcgaga cgatgaccgt
     7981 cggttgccac cgcacgcagc acagctcccg cctcaccggc aacgcaatgc agatagatgc
     8041 cgttgagata gtagcgcgtc tcttcggtcg agatggcgaa ctgggtcttt tcgatcagcc
     8101 ggcgcaactc cgtagccgga acatcgaagg catgcgacaa tacgccagcg gcaagatcag
     8161 gaaaatcacc ggccggcagt gattgcagca cgaagcgcga gcgcccggag cgcagcagca
     8221 gctgtcccgt gtcgccggtc tgctcaagcg agacctgcgc cccgtccggc agcttgcgca
     8281 cgatatcatg cagcacatgc gccggcacgg tggtggcgcc ggcctggctg acatcggcct
     8341 cgatcacctc ggtgacctcg atatcgagat ccgttgcctt gagctgcaac cgccgttcct
     8401 cggcgcggat cagcacattc gacaggatcg gtatggtgtt gcggcgctcg acaacgcgat
     8461 gcacatgtcc cagcgctttc agcagcgctg cctgttcgat cgtaaccttc atgccaaccg
     8521 tcctacaaac ctgcccatgc cagccgaggc agcatcccgg caagggtgca ggacgatgcc
     8581 gtctcttgcg cccggttgca agagcggatc ctgtcttggc tggggattac cgcgtcaaag
     8641 gcagtgccag accggcaacg gacccgttca ctcctgcaac atacgcttga ggtggtcgat
     8701 ctctccggcc agcgaggtat cgcgcgccac cagcccgtca atcttgcgaa ccgcatgcag
     8761 cacggtggtg tgatcgcggc caccgaaccg gcggccgatt tccggcagcg agcgcaaggt
     8821 cagcagcttg gccagataca tggcgatctg gcgggggcga accaccgtcg ccgtgcggcg
     8881 cgacgacagg atgtcggcac ggctgacgtt atagtgattg gcgaccagcc tctggatatc
     8941 ctcgattttg accttcttgg gttcatgggt gcggatcaga tcgcggatgg ccagctccgc
     9001 cccttccttg tcgagggggc cgcctgacag ggttgcatgg gcgagcaacc ggttgaccgc
     9061 tccttcgaga tcgcggccat tggccgtaac caattgcgcg acatgggcga tgacatccgg
     9121 cgcaatggca aaacccggca gccgcatccg ggcggtctgg atccgcgatt ccagcacctt
     9181 gagccgcagc gcctcatcca gcgcgccgat ttcgacgacc agtccgccgg ccagccgtga
     9241 ctggatccgc tcatcgagcg attcaagatc cgccggactg cggtcggccg ccacgatcac
     9301 ctgtttgccg gcatcgatca atgcattcag cgtgtggcag aattcctgct gcacctgctt
     9361 gccgttgaga aactgggcat cgtcaatgac caagagatcg atggcccgca gcgtttcctt
     9421 gaaggccagc gccgtctggc tgcgcagggc cgcaacaaag ccatacatga atttttcggc
     9481 cgtcagatag atcacccggc ggccattggc cgccaccgcc tgggccgtgg cctgcaacag
     9541 atgcgtcttg ccaagtccga cggccgaatg aacgaacagg ggattgtaga ccggcatgtc
     9601 cgctggtgcg gcctgaacga cctggagagc cgccgaatgg gcaagaatat tggatcggcc
     9661 gatatggaag gtcgaaaaca ccaggcgacg atccagcggc gaactctcga aataatcctg
     9721 agcccgtgtt ggcgtcgccc caggcggggt tgccgccaca acgacatcgg ccgctgcagg
     9781 ggcgctcgat ggcccaccgg caggcggact tgcgggagaa atgcgcgcca gaacaacttt
     9841 ttggcccgcc tcgcgcagcg cgatcgacac tttgcgcaca tcatcgcgct cttcggcgaa
     9901 cattgcctca atgcggtcga tatagtgacc atgcagccag ctgcgcagaa accgcgtcgg
     9961 aaccgacaga tgagccactc catcggcgat cgaaaccaga tcaagccggg cgaaccatga
    10021 ggtaaaaatg tcctcgccaa gttcggcgcg cagacgggcg cgaacccgtc cccagacggt
    10081 gtcggctcca tgcggctcga cctcggaggc tccagcggcg gcaggcagtg ctacgggtgt
    10141 cttttcggca aacatcaggc aacccaattc tgagaagatc atcagccggc agcaacccct
    10201 gtttggtccg ccaccagcga ggaattcaat ccggcgtcat cacgccagcc agtcaagtcc
    10261 attgtccgga gcgccatcag tcccggataa aagaatatag gttatccgag catagttcat
    10321 ttcaccctcg cattgatccc gcgccctttc tgattacaga aagagttttt cctacgaaaa
    10381 atctgtatta ccccaaacaa gcacgcacag aaccatgcct gttgcacaag caatgccttc
    10441 tgagctgatc ataggcgcaa gcaagactgc caggcctcaa ggagggtctg tcaatgcctt
    10501 gcttttgctg ctgtgtcggc ggccgttcgt ctgcccgcca aaagagtgtt agcggccccc
    10561 aagagggcgc gcaactgctt ttgtcccggg tctcggaggc cgatctctgg ccaagccccg
    10621 aggtcgcgcc agcctggacc ggtgcgccag ttttaaaccg ttgaggtcaa tgatttttct
    10681 gctgtggccg gttaagagaa atttaatgtt tcggaggcgt aacacgagac ttgtaacaaa
    10741 ttgtgatcgc cctctgccgg cgccggattt tcaccggtgt tgcctcttgc cggctaacat
    10801 cttgaaattt caacaaaaaa aatgagcgct cggtttcaga atcggcccga cgacaggaac
    10861 tgtgacatcg cgtcgattct tggggattga cacagattca ggatttacag cgccaatatt
    10921 ggttccgaca tgcgggtgtt tgactgcggt gcggccttca gcaacccaag gctatattgc
    10981 gccggaatcg tgcacttcag ctgtgtattc cgccagtata ggccctcatg tcttgatgga
    11041 tgcgccgatc atggcatcag gaggacgcgc tgcggaattg gccgcgccat ttccccagcg
    11101 tcgggcttgc aggtgtgggc tgacgggagg gcgaagccgc agtcttgccg cggtccttgc
    11161 aagaatgaaa accgatgatt gcaggacagg gagtgcttgc ccgggccata aaacaaaaaa
    11221 gccccggccg tagccagggc tgtcaacggt caggcaggct ggtcttcagg ccttgatcgc
    11281 cttgatcgcc ttcgtcaggc gcgagacctt ccgcgacgcg gtattcttat gcatcacgcc
    11341 gctgcgggca gcacgcatca tctccggctc ggcggcgcgc agggccgttg ccgcagcttc
    11401 ggcgttgccg gaagcaatcg cctcttcgac cttgcggatc gaggtgcgcg tgcggctgcg
    11461 gcgtgccttg ttgacttcgg tcttgcgcaa agcctggcgt gcggccttct tggcagactt
    11521 ggtattcgcc attgtttact tctccgggac aacctgccgg taaatgccga gcatcaaccg
    11581 agaccacatc ccaacgggtc gagacaaaaa aggagcgcgc acacccggtc gggtttgcgc
    11641 ttggcggtcc catggcacag gcaggggcct gcgtcaacgg ttgccggcgg gttttcttgg
    11701 attgcccgcc tcaggaccgc agccactggc caatcgggtg cgtcggatcg gccagtaggc
    11761 gcagcgccat caggatggag acaatcacca ccagcggctt gatcaacccg gccccgcgct
    11821 tcagcgcagc gcgtgcgccg atacgggcgc caagcattga cgcaaggccc agggtcaggc
    11881 cgagtgccca ccagacctgg ccggaaaatg cgaacaggcc cagcgcaccg gcgttgctgg
    11941 caagattgag gaacttggcg ttgccggcgg cgcgcgtgac gccctggccg agcagcagga
    12001 ccagcgagat gaaatagaag gtgccgccac cggggccgaa gatcccgtcg tagaagccta
    12061 tgaccagcgc cacgccgagc gcatagggca tcagacccag cctggcgcgc gaggcgacgt
    12121 cggaaagctt gggcgaggcc gcaacgaaca gggcaacgcc gatcaacagg accggaaccc
    12181 ctgtgcgcag cgcatcgatg ggaacgatct gcgccgaaac ggcgcccatc gcgcctccgg
    12241 ccagggaggc gaggaacgct ccccgcatcg ccacccggtc gatcagcccc gcccgcatgt
    12301 aggaccgtgt cgccgagatc gctccggcgg tgccctgcaa tttattggtg gccactgctg
    12361 caaccgggtt cagccccgcc agcatcaacg ccggcacatt gaggagtccg ccgccgcccg
    12421 ccacagcgtc gatcatggcg gccgtgaacg acaccgcgac cagccccgcg acgctggcaa
    12481 gcggcaggcc gtaaagctcg gcaagggtct cgatcatgac agcggaccct ggcgcatcgc
    12541 ggccggcagg tggcggtcag cgatgccgga aactggcttt gcgcttttcg gcgaaggcgg
    12601 ccattccttc cttctggtcg tcggtggcga acatggaatg gaacacgcgc cgctcgaagc
    12661 ggatgccttc ggcgagggtc gtctcatacg aacgattgac cgattccttc gtcatcataa
    12721 ccgctggcag cgacattgtg gcaatggtct cggcagcctt catggcttcg gagaccagct
    12781 gatcggccgg cacgatgcgc gagacgaggc cgcaacgctc ggcttcggcg gcatccatca
    12841 tgcggccggt gagacacaga tccatcgcct tggacttgcc gatataacgg gtaagccgct
    12901 gcgtgcctcc tgcgcccggc atgacgccga gcttgatttc cggctggccg aatttcgccg
    12961 tctccgctgc gataatgaaa tcgcacatca tggcaatctc gcagccgccg cccagagcga
    13021 aaccggcaac cgccgcaatc accggcttgc gcgtgcgcga aaggttatcc cagcggccga
    13081 tgaaatcgct gagatagacg tccatatagg tcttggattg catttccttg atgtcggcac
    13141 cggctgcgaa ggccttctcg gatccggtca gaaccagaca gccaatggcc tcgtccgcct
    13201 cgaagacatc cagtgcgtgg ttgagatcgg caatcagcgc cgaattgagg gcattgagcg
    13261 cctgcggccg gttcaggcgg atcaggccga cgcggccatg ggtttcggca atgatgttgt
    13321 cgtaggacgg tttcaggccc atggcggatc tccttgttac tgggattggc tagccgagct
    13381 tgcggagcgc ggcaagagcc gaaaggcgga gcgtgttctt tcaaaagccg aaaaggcgct
    13441 ggggcttact tctggcagcg gatgcaggag aaggtcgagc gacccgactg cacctgccgc
    13501 tggatgtgac cgtggcagcc gggcgtcggg cagggcgcgc cctcccggtc atacacacgg
    13561 aatgcatgct ggaagtagcc cagcgatcca tcggtgcgcg catgatcgct gatggaagag
    13621 ccgcccgcgg ccacggcctc ggtcagcaca tcacggatcg cgccagcgag acgctcgcgt
    13681 gcgcaggcag ccctgcgtct cgccccgctc agggttccgg ctggccgcgc cgggctcaac
    13741 cctgcccggt gcaatgcctc gcacacatag atgttgccaa gcccggcgat cagccgctgg
    13801 tcgagcagcg ccgccttgag aggtgcggcc ttgcccgcaa acagccggtc gatggccccg
    13861 gcatgaaaat cgttgctgag aggctcgatg ccgaggccgg ccagcaaggg atggtcgcaa
    13921 agcgcccccg cctcgaccag caccatgaag ccgaagcggc gtggatcatt gtagacaatg
    13981 cgtgcgcccg ccgcggtttc gaacacgacg tgatcatggg cggcaagacg ggggatggcc
    14041 tggtggaagg cgcccggctg gcgcgtctca tcgatctgct cgaccacaaa acgcccgctc
    14101 atcccaaggt gcaggatcag atgctcgccc gagccgagat cggcaatgag gtatttagcc
    14161 cgccggttga ggcgatctac ccgctggccg gtcagccgtt cgacaaagcg cggaacaaag
    14221 ggaaagcgca ggttcggccg gttgatccgg accgaggcca gccgctgacc ttcaagcacc
    14281 ggggcaagcc cgcggcgcac cgtttcgact tccggcagtt ccggcatggc ctcttgtccc
    14341 tccgcgtcaa ggatagctcc aagaacgcat agccttcacc gccatgtttc gctattgctt
    14401 ctcgccgaag tcaggaggag ccaccatggc ccgcgccgcc cgcccgtccg ccgcaagggt
    14461 tgccacttcg ccccgcgagg acgagtcgac ccatttcggc tttcaggatg tgccgctcgg
    14521 cgagaagcag ggccgggtca acgaagtctt tcattcggtc gccaagaaat atgacctgat
    14581 gaatgatctg atgtccgccg gccttcaccg ggcctggaag gatgcgatga tctcgatgtt
    14641 gcgcccgcca cgaggcgagg ggccgtatcg ccatctcgat gttgccggcg gaaccggcga
    14701 tgtggcgttt cgcattctca aggccggcgg ggcgggcatg gccgtcacgg ttctcgacat
    14761 caatggcgac atgctggcgg tgggtcgcga ccgggccgag aggcaggact tcgccggcgc
    14821 gatcgatttt gtcgaggcca atgccgaggc tcttccctat cccgacagga gctttgacag
    14881 ctacacgatc gctttcggta tccgcaacgt cccgcgcatc gatgtggcgc tcagggaagc
    14941 ctttcgtgtc ctcaagcgcg gcggccggtt cctctgcctc gaattctcgc cggtcgacat
    15001 gccggtgctc gaccgaattt acgatgccta ttccttcaac gtcattccgc tggtcggcca
    15061 ggcagtgacg ggtgatgccg acagctatcg ttacctggtg gagtcgatcc gcaagttccc
    15121 acgccccgtc ttgttcgccg acatgattcg tgcggccggg tttgggcggg tgacgatgcg
//
LOCUS       DAOMXG010000045        14975 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_2203_length_14975_cov_9.717158, whole genome shotgun sequence.
ACCESSION   DAOMXG010000045 DAOMXG010000000
VERSION     DAOMXG010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 14975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14975
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2203_length_14975_cov_9.717158"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1124
                     /locus_tag="PLQ11_04760"
     CDS             <1..1124
                     /locus_tag="PLQ11_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24247.1"
                     /translation="GLAVVLVVPGGARGLLAAAAVACVLVALGWLLPQASLLPLAAEA
                     RSDVLEAAVMRFAEAPVSAGDLIGWTCAGIGVLLLLDTALTGQRPLLARSGHAAAVLV
                     LLALAFLANLSDVGVRQAMQRLAATPVESLPAALFSERHRGEITICGTVARGVPDVMR
                     ACSGSIVDGRVPEARFAVNHPGSGRQLSAVHDLPTMVGVAFDLALPLVLVVTFALLVH
                     QAAKLFAHDIIYRLTGRTGTGSGRLAMQRLAALGLIAMIALGVRPAVDGELARALASL
                     ALFLAAAVPVPLVILARWGRADSRAALCGLASSLIAGVAFWTGYLPSGPGYLAGTAAG
                     LCAGMLAALVFPARSPHDRVAADILAGKQPGPLFQDKSA"
     gene            1121..2107
                     /locus_tag="PLQ11_04765"
     CDS             1121..2107
                     /locus_tag="PLQ11_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRK24248.1"
                     /translation="MSRDHSSGDLAADRRYLWAEASLKEGDAPAAADLFHQALALAPH
                     FSAAVFGLGSALERMGDVDGARTAYRRCLDQRPDDVFGAGLSLARLDGATPAAMPQDY
                     VARLFDDYAGRFESHLVGALHYRGPAILVEALEAVTAAQQRPCRFLQALDLGCGTGLM
                     ADALGGRVQWIDGVDLSSGMLAQAAARGHYRSLIQADITAALAMLPAGQSYDLVIAAD
                     VLVYLGDLGDLFRAIAAALAPGGLFAFTVQAGPDGGYALGGDLRFSHGAGYLGAVAGA
                     AGLRLTHLAPAVTRKDAGRDVAGYAAVLESADQPPRTTGRDSSCVQSVMDPS"
     gene            complement(2044..2877)
                     /locus_tag="PLQ11_04770"
     CDS             complement(2044..2877)
                     /locus_tag="PLQ11_04770"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HRK24249.1"
                     /translation="MTAANGLISAATLAQRLGSDLSILDARSASMFEAGHIPGAINSD
                     YAADGWRARVGMVPGLLPGVEHLAGLFSRLGLAPTRAIILVPAGQSVSDLSATARMAW
                     TLDLCGYRVEALLDGGMTAWMAAGLPVQKGAVQPVPGSGALHIDTSTRATATDVAQAL
                     ADGRTTFVDARSRSYFDGQEKAPEARAAGHLPGAVSVDYASLYDPAQQRLRPRAELAR
                     IFSMLPTDRPVISYCNTGHTAALNWFVLSRVLDRADVRLYDGSMTDWTQDESRPVVRG
                     G"
     gene            complement(2874..3305)
                     /locus_tag="PLQ11_04775"
     CDS             complement(2874..3305)
                     /locus_tag="PLQ11_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRK24250.1"
                     /translation="MKASVKWVDGMMFLGESGSGHSVVMDGAPESGGRNHGIRPMEML
                     LIGLGGCTAFDVVLILKRGREPVEGCTIEISAERAESDPKVFTTIHLNYRVSGRGLNP
                     AKVERAIALSKEKYCSASIMLGATATMTHEFSVSEARDLIG"
     gene            complement(3431..4702)
                     /locus_tag="PLQ11_04780"
     CDS             complement(3431..4702)
                     /locus_tag="PLQ11_04780"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HRK24251.1"
                     /translation="MLRPISRRAFTAGAALAGLAGLASGLCASEPRAPFRRGVSIHNL
                     MNWGQLEPNDKTRYRWPPFASVHHQLQPQIYDNLVEAGFDFVRLTLDPGVFLQASGQH
                     RADLDAQLLTHVKKFQGLGLGVIVDFHPNSQVPDYAPEKITENANSALFKDYGAMLER
                     TALLLKPLTGSRVAFELMNEPQWGWDGGTAARWQKMLEIWHERVRSVAPDLMLVLTGA
                     RGGNIDGLRLMDAKPFAGSNTLWTFHYYEPYELTHAGVHFTDPKARTRKYFSDLPYPG
                     APEHLEDAMRIIAGNIGADTALSAAQKKELTSEARTALAKYLEARPGRKTIKAQFDTV
                     VDWAKRNNIANNHIFLGEFGVTRTYGRYRASPPAPLKNWLSDVREEAEARGFGWALWV
                     LSGYGGMSLIETDESVRFDGPTLEALGLKAG"
     gene            complement(4707..6038)
                     /locus_tag="PLQ11_04785"
     CDS             complement(4707..6038)
                     /locus_tag="PLQ11_04785"
                     /inference="COORDINATES: protein motif:HMM:NF038256.1"
                     /note="VpsF, distantly related to oligosaccharide ligases,
                     is encoded next to the probable flippase VpsE; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VpsF family polysaccharide biosynthesis protein"
                     /protein_id="HRK24252.1"
                     /translation="MRTVYRGSSSQRIAPPAPAPTFGLPVVEASGEVDTSRFVTGVAY
                     IFAVLLVLRLLLTDSVLNQFINYTGEGGFILEKIHPCTQAIVLLLFITLAKVKVSLTP
                     SEALIIRQMCILMAVIVALVMLVQVMGRPIAIGYLLDTYIGACLVCFLMFALPLRHRR
                     MIGHVIMAYLVVNSLVAIVEKATSFRLMPYPYVETVFRPTALTAHPLAVGLFSAGGAA
                     FTLATGWRPWVKGLALLAFLLGTAASGARTGLIFTTLSILGALVLTDVGGQTGEQRLQ
                     RRLLLFGSMAFGGVLLFSIGLAAGFLERFEGGYIDENASARIDVYQVFDWVSWKDILF
                     GADMLEIKAMVKDRLDLLIESSVVVFVFQFGLFGAITLAATIFWTVWRISSASDWRGV
                     VASVTFCAVAMSNDTLSGKHFHFAVLFLCLIAFRNNSHDHLGRRVRPNWEA"
     gene            complement(6041..7477)
                     /locus_tag="PLQ11_04790"
     CDS             complement(6041..7477)
                     /locus_tag="PLQ11_04790"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HRK24253.1"
                     /translation="MAHQIETLAVAAASHKRILSPPLVTLIRIVSEASVVFLAAYASG
                     VAYHVAVNRSLAYTNPQMDLAATFTVLFVLISFYRDDYRLSLYQKTRKAAQSVVQSFA
                     LALVAVIVLAFVTKSSESFSRGSVLLFLPTGLASLIGARLMISKAVRRVTRSGWVSAG
                     RVMVVGFADELDWVLHKGSPQRMGFDVIGVEQLADERGDQQPIPGIRIDAIVDKARFL
                     NPDTIVLALPWSRSDLIEQCVRAFMNLPARVELAPERVLERYSELRLGRLGAMLTLNV
                     LRPPLGHAGRATKRLLDIVLAMTALLLLAPLFAVVALLIRLDSPGPALFRQTRYGFNQ
                     KPFLIWKFRTMHTQDNGPVVQQASRNDARITRIGKHLRRLNIDELPQLFNVLIGEMSL
                     VGPRPHAVAHNRFYEGKIDLYARRHNVLPGITGWAQVNGLRGETDDAAMRARVDLDLY
                     YLDNWSLSFDLIIIVMTILSKKSYRNAY"
     gene            7705..9975
                     /locus_tag="PLQ11_04795"
     CDS             7705..9975
                     /locus_tag="PLQ11_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24254.1"
                     /translation="MVIRYASETGPDASEPKRIRPLAPVLAVDSLGLRLSGLARAALA
                     RLRRRLLAMILVAALPLAGAFAYLKVMPPVYLATASVFVDPAGGRPGRSDSPVDPAII
                     AAHLRQVTAKSTFQRAIDKDRLAEDSSIHARPTGMAALARIVLDLINRSQDNRSENRN
                     ETLLRVLAEQISARRSGEANLIEIVARSSDGDTAARLANAVAQAFVDEVVAGAGSVQG
                     AERARAAALGDDALKKLREAEAKLADFRARNGIEPAGQGKPGAQDGDIAAQLTRARAA
                     SLEAKTRSDQIQKLLASGKDIEAIADLVRSPAIDRLRIQYNEAAAQEASFRTSLGPRH
                     PSYLEAAEQARERRRLLMEGLRLAGSSARSDWQAARDQELALERRAGTDAPAQSAAAP
                     AEPPALLRELERAVDLARLAYERTLKTQQSAESAPDQGAARVVTRASPSDEPSRIEAG
                     VVWSRAGMASAGLSALVLLLGGGGKRSGPSGSVKPSRRSRTVPVRDMQVAGEMATPPA
                     DQTGAGRVTAEQVARGWAEVVERTFGAAPAGGAPASAPPPAEPPTASQAVAREIAARD
                     GEFALQLVLVTADVPSPQQGEIALDLARASAQLDLRVLVVDANPGDQELSRRFAAAPC
                     KGRLHLNGRDRPLLQAELGAASVAMVPNEQVRGLPANDASGTHIKSLAGHFDLVIING
                     APLTDAAAVRALARTAQSIVVAMSNSAGPGQAQIAALLGTSASQVRIVPTGSPPAPPL
                     DARNRPMAVVRLSQCA"
     gene            9966..11480
                     /locus_tag="PLQ11_04800"
     CDS             9966..11480
                     /locus_tag="PLQ11_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24255.1"
                     /translation="MRLIPVSQPALRHVTVEVRVPRRACRRWHRWLVEALLAQGHRVS
                     LRICDGPADEVAGLELLGFVERMLYQHQLGEETDASAVIEARDWVGGPAADRPEVIFD
                     CSTTDGPYPETGEVLLPSFDGMRSLAGLVGSLIGQREPLIGWRRKSDGALVAGGRAAL
                     AARHILTATLAQSLALMPGLGARALADTMQAGAVTAPGAVSANLPAGHLAITRFGLAN
                     LADRLSRRLRETLTTPRQWSVAWRRVAPEQGLVAAASFDPTCYTWLPRDPARYFADPF
                     LFEHQGGMFLFCEEFPHATGRGLISVMEWTGNGFGTPRPVLERPYHLSYPCVFRHGSE
                     IWMIPETTAAGTLELYRATDFPDRWSLHGVIAEGINAADATVFKHDGLIWIAAGTAAP
                     GSSDRDQLSLFFAESLEGPWQQHPLNPVVVDAFGARPAGGWQSTPSGWRRPAQDCSGG
                     YGWGLSLRRIDRLDRERFAETELIRFPPPAVLDATGFHTVNVGGGLETIDALKP"
     gene            complement(11470..12903)
                     /locus_tag="PLQ11_04805"
     CDS             complement(11470..12903)
                     /locus_tag="PLQ11_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRK24256.1"
                     /translation="MSETFSSFLAELAATIGPAQISTDPNDLAPHLVEGRGLYRGTAF
                     GLARPRDTDDVSACLWLANRHRLPVVAHGGNTGLVGGGVPRDALVLSLDRMNRIRAVD
                     PVNLTMTVEAGAILKTVQDAATAEGCLFPLSLGSEGSCTIGGNLSTNAGGTAVLRYGN
                     ARELALGLEVVLADGRVLNDLNRLRKNNTGYDLRNLFIGAEGTLGIITAAVLKLFPAP
                     RVRATAWVGLDSRQAGLDLFDLVRASAGECLTGYEFMYAEGIDFVLRHIPGTRCPLDS
                     RSPFHVLIELSSSDASLDLAGRLESLLGDAYERGLIGDATIAASVAQAAALWSLREHI
                     SDAQKFEGGSIKHDISVPVSAVVAFLDRATAACEAELAGLRVVAFGHFGDGNIHFNLS
                     QPVGMDKAEYLSMWDRFNRIVHDIVADLGGSISAEHGIGLIKLHELARYKDPVALDLM
                     ARLKATLDPHNILNPGKVLPPRQSFTA"
     gene            complement(12953..13984)
                     /locus_tag="PLQ11_04810"
     CDS             complement(12953..13984)
                     /locus_tag="PLQ11_04810"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HRK24257.1"
                     /translation="MSIRHGLIGAIGNTPLIRLRHASEATGCTILGKAEFMNPGGSVK
                     DRAALAILEDGRASGALKPGGVIVEGTAGNTGIGLALVGNALGHRTVIVIPETQSREK
                     KDMLRHAGADLVEVPAAPYANPNNYIKVSARLAEQLNRSEPHGAFWANQFDNTANREG
                     HRRTTAREIFEQTDERIDGFVCAVGTGGSLAGIGLGLKERNPAITIALADPMGAALHS
                     YFTTGELKAAGSSVTEGIGQGRITRNIEGAPVDVSFQIPDEEAIPVMFDLLALEGLCV
                     GGSSGVNVAGAIRLARHLGPGKTIVTLLCDGGTRYASKLYDPAFLESKGLAVPAWLRP
                     RPSIDPGLL"
     gene            complement(13996..14460)
                     /locus_tag="PLQ11_04815"
     CDS             complement(13996..14460)
                     /locus_tag="PLQ11_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011438909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRK24258.1"
                     /translation="MMDMIDRKLLTLLQQDANQSLAELSERVGLSQTPCWKRIQKLEA
                     TGIIAKRVALLNPDKIGLGLTVFVSIETGDHSGPWLERFADIVSAMPEVMGLYRMAGD
                     VDYLAHVVVADMAAYDQFYKRLINTIALKNVTSRFAMERIKSTTAFIIPPSA"
     gene            14700..>14975
                     /locus_tag="PLQ11_04820"
     CDS             14700..>14975
                     /locus_tag="PLQ11_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRK24259.1"
                     /translation="MTRPDRPPLDCSVLHAETRLVHEGILRSGFGETSEALFLTQGHV
                     YDNAEQCEARFAGADPGFIYARFSNPTVQMFERRMAGLEGAEAARATA"
BASE COUNT         2586 a         4698 c         4922 g         2769 t
ORIGIN      
        1 cgggcctggc ggtggttctg gttgtccctg gcggggcgcg cggattgctg gcggcagcgg
       61 ccgtggcctg tgtgctggta gcccttggct ggctgctgcc gcaggcttcg ctgctgcctc
      121 tggccgccga ggcgcgatcc gatgtgttgg aagctgcggt tatgcgcttt gccgaggcgc
      181 cggtgtcggc aggggatctc atcggctgga cctgtgcggg catcggcgtt ctgctgctgc
      241 ttgatacggc gctgaccggg caaagaccgc tgcttgcccg gtccggacat gcggctgcgg
      301 tgcttgtgtt gctggctttg gcctttcttg ccaacctgtc cgatgtcggc gtgcggcagg
      361 ccatgcagcg actggccgcc accccggtcg aatccctccc cgcagccctg ttttcggaac
      421 gccaccgtgg cgagatcacc atctgcggaa cggttgcgcg cggcgtgccg gatgtcatgc
      481 gggcctgctc cggctcgata gtggacgggc gtgtgccgga agcgcgcttt gccgtcaacc
      541 atcccggctc cggccgccag ctgtccgctg tgcacgatct gccgacgatg gtcggcgtcg
      601 ccttcgatct tgcgctgccg ttggtgctgg tcgtaacgtt cgccctgctt gttcatcagg
      661 ccgccaaact cttcgcccac gatatcatct atcggctgac gggacgcacc ggaaccggca
      721 gcggtcggct ggccatgcaa cggctggcag cgctggggct gatcgccatg atagcgctgg
      781 gggttcgccc ggcagtcgac ggggagctgg ccagagcgct ggcgtcgctt gccctgttcc
      841 tggctgcggc ggtaccggtg ccactggtca ttctggcgcg ctggggccgc gccgattcgc
      901 gcgcagcctt gtgcgggctg gccagttcgc tgatcgccgg cgtcgcgttc tggaccggct
      961 atctgccatc cggtcccggt tatcttgccg gcacggcggc gggactatgc gccggaatgc
     1021 tggctgccct ggtttttccg gcgcgctcgc cgcacgatcg cgttgctgcc gacatccttg
     1081 ccggaaagca gcccgggccg ctgtttcagg acaagagcgc atgagccggg atcactcctc
     1141 gggcgatctg gccgccgacc gccggtatct ttgggccgag gccagcctca aggaggggga
     1201 cgctccggcc gctgccgatc tcttccatca ggctttggcc ttggccccgc atttttctgc
     1261 tgcggtgttc gggctgggat cggctctgga acggatgggc gatgtggacg gcgctcgcac
     1321 agcctatcga cgctgtctgg accagcggcc tgacgacgtg tttggcgcag gcctttcgct
     1381 ggcccggctc gacggcgcga ctccggcagc catgccgcag gactatgtcg cccgcttgtt
     1441 tgacgattat gccggccgct tcgaaagcca tcttgtcggt gcgctccact atcgcggccc
     1501 ggccattctc gtggaggcgc tggaggcagt gaccgcagcg cagcagcggc cctgccggtt
     1561 tctacaggcg ctggatctgg gctgtggcac gggattgatg gccgatgctc tcggcgggcg
     1621 ggttcaatgg atcgacggcg tggacctgtc atccggcatg ctggcccagg ctgccgctcg
     1681 gggccactac cgcagcctga tccaggccga catcaccgca gctctggcca tgctgcctgc
     1741 cggccagagc tacgatctgg tgatcgctgc cgatgtcctg gtctatcttg gtgatcttgg
     1801 cgaccttttt cgggccatcg ccgctgcatt ggcgcccggc ggtctgttcg ccttcaccgt
     1861 tcaggcggga cccgacggcg gctatgccct tggtggcgat ctcaggtttt cccatggtgc
     1921 cggctatctt ggcgccgtgg ccggggcggc tgggctcaga ctgacccatc tcgcccctgc
     1981 cgtgacccgg aaggatgccg gccgcgatgt tgctggctac gccgcggttc tggaaagcgc
     2041 cgatcagcca ccgcgcacga ccggccggga ttcgtcctgc gtccaatccg tcatggaccc
     2101 atcgtagagc ctgacatcag cgcgatcgag aacccgggac agcacgaacc agttcagcgc
     2161 tgccgtgtgg ccggtgttgc aatagctgat caccggcctg tcggttggca gcatcgaaaa
     2221 aatccgggcc agctctgccc gcggtctcag gcgctgctgc gccggatcgt agaggctggc
     2281 gtaatcgacc gatacggcgc cgggcagatg gccggcggcg cgcgcttcgg gtgccttttc
     2341 ctgcccgtcg aaataggatc gggagcgggc atcgacgaac gtcgttcgac cgtcagccag
     2401 cgcttgggca acatccgttg ccgttgcccg ggtgctggta tcgatgtgca gggctccgct
     2461 gcccggcact ggttgcacgg cgcccttctg cacaggcagg ccggcggcca tccatgcggt
     2521 catgcctcca tcaagcaggg cttcaacccg atagccgcaa aggtccagag tccaggccat
     2581 gcgggcggtt gcggaaagat cgctcaccga ttggccggcc gggaccagga tgatggcccg
     2641 tgtcggggca agtcccaacc ggctgaacaa gcccgcgagg tgctccactc cgggcaacag
     2701 tcccggcacc attccgacgc gcgcccgcca gccatcggcg gcataatcgc tgttgatggc
     2761 gccgggaata tgcccggcct cgaacatgga ggcgctgcgc gcatcgagga tcgaaagatc
     2821 actgccgagc cgctgtgcca gcgttgcagc gctgatcaat ccattcgcgg cggtcatccg
     2881 atcagatccc tggcttcgga aacggaaaat tcgtgcgtca tcgttgccgt tgcgcccagc
     2941 atgatcgatg cggaacagta tttctccttc gacagggcga tcgcccgttc aacctttgcg
     3001 gggttgagcc cgcgcccact gacgcgatag ttgagatgga tggtggtgaa caccttggga
     3061 tcgctctcgg ccctttcggc gctgatctcg atggtgcagc cttcgacagg ctcacgcccg
     3121 cgcttgagaa tcaggacgac atcgaaggcg gtgcaaccgc caagcccgat cagcagcatt
     3181 tccatcgggc ggataccgtg attgcgcccg ccggattcgg gagcgccatc catcacgacc
     3241 gaatgcccgc tccccgattc gccgaggaac atcattccgt ccacccattt cacactcgcc
     3301 ttcatgtctg ttcccctgat cccgccattc cgtcaggtgc aaaggtgcgc gcaaccccgc
     3361 cgccggtcaa tgccagcacg gcggacgcgg tgacgcagaa gccagggggg agccccagcg
     3421 gcaggatcgt tcagccagcc ttgagcccga gcgcttccag cgtcggtcca tcgaaccgca
     3481 cgctttcatc ggtctcgatc aacgacatcc cgccatagcc ggacaggacc cagagcgccc
     3541 agccgaagcc gcgtgcttca gcctcctcgc gtacgtcgga aagccagttc ttcagggggg
     3601 cgggcggaga cgcccgatag cggccatagg tgcgggtgac gccgaattca ccgaggaaga
     3661 tgtggttgtt ggcaatgttg ttgcgcttgg cccagtccac cacagtgtcg aattgggcct
     3721 tgatcgtctt gcggcctggc cgggcctcga gatatttggc cagtgcagtg cgtgcctcgg
     3781 acgtcagttc tttcttctgc gccgccgaca gggcggtgtc tgctccaatg ttgccggcga
     3841 tgatgcgcat ggcatcctcg agatgctccg gggcgcccgg atagggcaaa tcggaaaaat
     3901 acttccgcgt tctggccttc ggatcggtga aatggacgcc ggcatgggtc agttcgtagg
     3961 gctcgtaata gtggaaggtc cagagggtgt tgctgcctgc aaagggtttg gcatccatca
     4021 gcctgagacc gtcgatgttc cctccgcgcg caccggtcag caccagcatc aggtccggcg
     4081 ccacgctccg cacgcgttcg tgccagattt ccagcatctt ctgccagcgt gccgctgtgc
     4141 cgccgtccca tccccattgc ggttcgttca tcagttcgaa tgcgacacgg cttccggtca
     4201 atggcttgag cagcagcgcg gtccgctcca gcatcgcgcc ataatccttg aacagggcgc
     4261 tgttggcgtt ttcggttatt ttctccggcg cgtagtcagg cacctggctg ttgggatgga
     4321 aatcgacgat gacacccagc cccagcccct ggaacttttt cacatgagtc agcaattgcg
     4381 cgtcgagatc ggcgcgatgt tggccggaag cctgcaggaa aacgccggga tctagcgtca
     4441 gccggacgaa gtcgaaccct gcctccacca gattgtcgta gatctggggt tgcaactgat
     4501 gatgaacgga tgcgaagggc ggccagcggt atctggtctt gtcgttcggc tccagctggc
     4561 cccagttcat caggttgtgg atgctgacgc cacgcctgaa tggcgcgcgc ggttcgctgg
     4621 cgcaaagacc ggacgccagc cctgccaatc ccgcgagcgc agcgccggcg gtgaatgcac
     4681 gtcgggaaat cggacgcagc atggtttcat gcctcccagt ttgggcgaac ccgtctgccc
     4741 agatggtcat ggctgttgtt cctgaatgcg atcagacaca ggaacaggac cgcaaagtga
     4801 aaatgcttgc ctgacagggt gtcgttgctc atcgccaccg cgcagaaggt cacggacgca
     4861 accacgccgc gccagtccga ggcagaggag atccgccaga cggtccagaa gatcgtggct
     4921 gcaagggtga tggcgccgaa cagcccgaac tggaatacga acaccacgac cgagctttcg
     4981 atcagcagat ccagccgatc tttcaccatg gccttgattt cgagcatgtc ggcgccgaac
     5041 aggatgtctt tccagctcac ccagtcgaag acctgataga catcgatgcg ggcggaggcg
     5101 ttttcgtcga tatagccccc ctcgaaacgc tcgagaaatc cggcagccag gccaatgctg
     5161 aataagagaa ctccgccgaa ggccatggac ccgaacagca gcaaccgccg ctgcagccgc
     5221 tgctctccgg tctgtccgcc gacatcggtc agcacgagcg cgcccaggat cgaaagggtg
     5281 gtgaagatga ggccggtgcg cgctcccgag gctgccgttc caagcagaaa tgccagcagc
     5341 gccagtccct tcacccatgg ccgccagcct gtggccaggg taaacgcggc gccgcccgcg
     5401 ctgaacaggc cgactgccaa gggatgggcg gtgagcgccg tcggccggaa cacggtctcg
     5461 acatagggat agggcatcag ccggaaggat gtggccttct cgacgatcgc gaccagcgaa
     5521 ttgaccacaa gataggccat gatcacatgg ccgatcatcc gcctgtgccg gagcggcaaa
     5581 gcaaacatca ggaagcagac aaggcaggcg ccgatatagg tatcgagcag atagccgatc
     5641 gcgatcggcc ggcccatgac ctggaccagc attaccagcg cgacgatcac cgccatcagg
     5701 atgcacatct gccgaatgat caacgcttcg gatggcgtca gcgacacctt gaccttggca
     5761 agcgtgatga acagcagaag cacgatcgcc tgggtgcagg ggtggatctt ctcgaggatg
     5821 aagccgcctt caccggtata gttgatgaac tggttgagca ccgaatcggt caaaagcagc
     5881 ctgagcacca agaggacagc gaagatatag gcgacgcctg tcacaaagcg gctggtatcg
     5941 acttcgccgg atgcctcgac aaccggcaag ccgaacgtcg gcgccggggc aggcggggct
     6001 atccgctggg aggatgatcc gcgatacacg gtgcgcatga tcagtaggcg ttccggtagc
     6061 tctttttgga aaggatggtc atcacgatga tgataaggtc gaaacttaag gaccagttat
     6121 cgagatagta gagatcgagg tcgacgcgcg cccgcatggc ggcatcatcg gtctcgccac
     6181 gcagcccgtt gacctgtgcc cagccggtga ttccgggaag aacattgtga cgccgggcat
     6241 agaggtcgat cttgccctcg tagaagcgat tgtgcgcgac ggcatgcggg cgcgggccta
     6301 ccagcgacat ctcgccgatc agaacgttga aaagctgcgg cagttcgtca atgttcagcc
     6361 ggcgcaggtg tttgccaatc cgggtgatgc gcgcatcgtt gcggctggcc tgctggacca
     6421 ccgggccgtt gtcctgggtg tgcatggtcc ggaatttcca gatcaggaag ggcttctggt
     6481 tgaagccgta gcgcgtctgg cggaacagcg ccgggccggg gctgtcgagc cggatcaaga
     6541 gggcaacgac ggcgaacagc ggcgccagca gcaggagcgc tgtcatcgcc agcacgatat
     6601 cgagcaggcg tttggtcgca cggccggcgt gcccaagcgg cgggcgcagc acattgagcg
     6661 tcagcatggc gccgaggcgg ccaaggcgca gttcggaata gcgttcgagc acgcgctcgg
     6721 gggcgagttc gacgcgcgcc ggcaggttca tgaaggcccg cacgcattgc tcgatcaggt
     6781 ccgatcgcga ccacggcaac gccaggacga tcgtgtcggg attgaggaac cgcgccttgt
     6841 cgacgattgc atcaatcctg atcccgggga tcggctgctg gtcgccacgc tcgtcggcaa
     6901 gctgctcgac gccgatcacg tcaaacccca tgcgctgggg tgagcccttg tgcaggaccc
     6961 agtcaagctc gtcggcaaat ccgacaacca tcacccgtcc ggccgaaacc cagccggatc
     7021 gggtcacccg cctcaccgcc ttgctgatca tcagtcgcgc accgatcagg ctggcaagtc
     7081 cggtcggcag aaacagcagg accgaaccgc gcgagaaact ttcggagctc tttgtgacaa
     7141 aggccagcac gatgacggcg accagggcaa gagcgaagga ctgtaccacg ctctgggctg
     7201 ccttgcgcgt tttctggtag aggctgagac ggtaatcgtc ccgatagaag ctgatgagga
     7261 caaacagcac cgtgaatgtg gctgccaggt ccatctgtgg attggtatag gccagcgaac
     7321 ggttgaccgc gacgtggtag gcaacaccgc tggcataggc ggcaagaaag acaacactcg
     7381 cttcgctgac gatgcggatc aaagtgacca gcggcgggct cagaatccgc ttatgggacg
     7441 cggcggcaac ggcaagtgtt tcgatttgat gcgccatggg acacctgaac tggattgtag
     7501 ccttcggtgt gcgaatccca tgccgatgcg ggttggagat cgtcagggcg agcggggagg
     7561 cggggactgc cccaaattgg caccccggta aacacttcat aaaggtttgg cggatgatga
     7621 tcggcgaggt tcgattgatt ctttgaccgg ctggcatcga gagcttcggc accgttctcg
     7681 agcagctggt atccggtgag ggtgatggtc attcgctacg cttccgagac gggtccggat
     7741 gcttccgagc cgaagcggat ccggccgctg gcgcccgtac ttgcggtcga cagccttgga
     7801 ctgcggcttt ccggtttggc acgcgctgca ctggcacgcc tgcggcgtcg tctgctggcg
     7861 atgatcttgg tggcggcatt gccgctcgcc ggagcctttg cctatctcaa ggtgatgcca
     7921 ccggtctacc ttgccaccgc ttcggtgttt gtcgatccgg ccggcggtcg gccgggcagg
     7981 tccgacagtc cggtcgatcc tgcgatcatc gcggcgcatc tgcggcaggt gaccgccaag
     8041 tccacgttcc agcgggccat cgacaaggac cgtctggccg aggattcatc gatccacgcc
     8101 cggccgaccg gcatggcggc gctggcacgc atcgtgctcg acctgatcaa tcgttcgcag
     8161 gacaaccgct ccgagaaccg caacgagact ctgctgcgtg ttctggccga gcagatcagc
     8221 gcccggcgct ccggtgaagc caatctgatc gagatcgtcg cccggtcgtc ggatggggac
     8281 acggcagcgc ggctcgccaa tgcagtggcg caggcatttg ttgatgaggt tgttgccggg
     8341 gctggcagtg tgcagggtgc cgaaagggca cgcgccgcgg ctctcgggga cgacgcattg
     8401 aagaagttgc gggaggcgga agcgaaactg gctgatttcc gggcacgcaa cgggatcgag
     8461 ccggccggcc agggcaagcc cggcgcccag gacggcgaca ttgccgccca gttgacgcga
     8521 gcccgcgcgg ccagcctgga ggccaagaca cgctccgacc agatccagaa gctgcttgcc
     8581 agcggcaagg acatcgaggc cattgccgat ctcgtccgct cgccggccat cgaccgtctg
     8641 cgcatccaat acaacgaggc tgcggctcag gaagccagtt ttcgcacctc gctcgggccc
     8701 cgtcacccgt cctatctgga ggcagccgaa caggcgcgcg agcgccgcag gctgctgatg
     8761 gaaggcttgc gcctggctgg ctcgagtgcc cgttccgact ggcaggctgc gcgggatcag
     8821 gaactggccc tcgagcgacg cgccggcaca gatgcgccgg cacaatccgc tgccgctccg
     8881 gcggagccgc cagcgctgct gcgtgaactt gaacgggccg tcgatctggc aagactcgcc
     8941 tatgagcgaa cactgaagac ccagcagagc gctgaatcgg ccccggatca gggggcggcc
     9001 cgcgttgtca cgcgcgcctc gccgtcggat gaaccgtccc ggatcgaggc tggcgtggtc
     9061 tggtcgcggg ccggcatggc ctcggccggg ctgtcggctc tcgtcctgct gctcggcggc
     9121 ggaggtaagc gttcgggccc gtccggttcg gtcaagccat cccgccgtag caggaccgtt
     9181 ccggtcaggg acatgcaggt ggctggcgaa atggccacgc ccccggccga tcaaacaggg
     9241 gccggcaggg tcacggcaga acaggttgcc cggggctggg ccgaggttgt cgagcgcacg
     9301 tttggtgccg cgcctgccgg aggggctccg gcatctgcgc cgccgccggc tgagccaccg
     9361 accgcatcgc aggctgttgc ccgcgagatc gccgcccgcg acggcgaatt cgcactccag
     9421 ctcgtgctgg tgactgccga tgtcccctcg ccccagcagg gagaaattgc cctggatcta
     9481 gcccgggcat cggcccagct tgatctgcgc gtgcttgtgg tcgatgccaa tcccggggac
     9541 caggaactgt cccgtcgctt tgctgcggcg ccctgcaagg gccgcctgca tctcaacggg
     9601 cgtgaccggc cattgctgca ggccgaactt ggcgcggcaa gcgtggcgat ggtgccgaac
     9661 gagcaggtca ggggcttgcc cgccaatgac gcgagcggga cgcatatcaa gagccttgcc
     9721 ggccatttcg acctggtgat catcaacgga gcaccattga ccgatgctgc cgcggtgcgt
     9781 gcgctggccc gcacggcaca aagcattgtg gttgccatga gcaactctgc cggccccggt
     9841 caggcccaga ttgccgcctt gctggggacc tcggccagcc aggtgcgcat cgtgccgacc
     9901 ggttcgccac cggccccgcc actcgacgcc cgcaatcggc cgatggcggt tgttcggctc
     9961 tcgcaatgcg cctgattcca gtatcgcaac cggcactgag acatgtcacg gtagaagtgc
    10021 gtgtgccccg gcgggcctgc cgtcgctggc accgctggct ggtcgaggcg ctgctggcgc
    10081 agggccaccg cgtcagcctg cgcatctgcg acggcccggc cgatgaggtt gccgggctcg
    10141 aattgctcgg ttttgtcgaa cgcatgctct atcagcatca gctgggcgaa gagaccgatg
    10201 caagtgccgt catcgaggcc agggactggg tcggcggtcc ggcagcggat aggccggagg
    10261 tcattttcga ctgttccaca accgatgggc cctatccgga aaccggtgag gtgctcttgc
    10321 cttcctttga cggcatgcgc tcgctcgcag gtcttgtagg gtcgttgatc gggcagcggg
    10381 aaccgttgat tggctggcgc cgcaagagcg acggggcgct ggtggctggc gggcgggcgg
    10441 cactcgcggc ccgtcatatc ctgaccgcga cattggccca gtcgctcgca ttgatgccgg
    10501 ggctcggagc ccgcgccctg gccgacacaa tgcaggctgg cgcggtaacg gcgccgggcg
    10561 cagtgtccgc caacctgccc gcagggcacc tggccatcac ccgcttcggt cttgccaatc
    10621 tggctgatcg tctaagccgc cgtttgcggg aaacgctgac gacgccgcgc cagtggagcg
    10681 tcgcctggcg ccgcgtggcg cctgagcagg ggctggttgc ggccgcgtcc ttcgatccga
    10741 cctgctacac ttggcttccg cgtgatccgg cgcgctactt tgccgatccg ttcctgttcg
    10801 agcaccaggg tgggatgttc ctgttctgcg aggaatttcc ccacgcaacc ggtcgcgggc
    10861 tgatctcggt catggaatgg accggcaatg gcttcggcac gccccgcccg gtgctggaac
    10921 ggccttacca cttatcgtat ccatgcgtct ttcggcatgg cagcgagatc tggatgatcc
    10981 ccgaaacgac ggcggcggga acactggagc tttaccgtgc cacggacttt cctgatcggt
    11041 ggagcctgca tggcgtgatc gccgagggca tcaatgctgc ggacgcgacc gtgttcaagc
    11101 atgacggtct gatatggatc gccgccggga cggcggcccc gggctcgtcg gatcgtgacc
    11161 agctgtcgtt gttctttgct gaatcgctgg aaggcccctg gcagcagcat ccgctcaatc
    11221 ctgtggtggt cgatgccttc ggcgcccgtc ctgccggcgg ttggcaaagc acgccgtccg
    11281 gatggcggcg cccggcgcag gattgttcgg gtggctacgg ctggggcctg tcgctccgcc
    11341 ggattgaccg gctggaccgg gagcgttttg ccgagacaga gctgatccgc ttcccgccgc
    11401 cagccgtgct ggatgccacc ggattccaca ccgtcaacgt cggcggcggc cttgagacga
    11461 ttgacgcgct caagccgtga aggactgccg gggcggcaga accttgcccg ggttgaggat
    11521 gttgtggggg tcgagcgtcg ccttcagacg tgccatgaga tcgagagcga ccggatcctt
    11581 gtagcgcgcc agttcgtgca gtttgatcag gccgataccg tgctcggccg agattgatcc
    11641 gcccaggtcg gccacgatgt catgcacgat gcggttgaag cgatcccaca tcgacagata
    11701 ctcggccttg tccatgccga ccggctggct gagattgaaa tgaatgttgc cgtcgccgaa
    11761 atggccgaag gcgacgacgc ggagtccggc cagttcagcc tcgcaggctg cggttgccct
    11821 gtccagaaat gcaacgacgg cggagacagg cacggaaatg tcgtgcttga tcgaaccacc
    11881 ctcgaatttc tgggcatcgg aaatatgttc acgcaggctc cacagtgcag cggcctgggc
    11941 cacgctcgcc gcaatggtcg catcgccaat caggccacgc tcgtaggcat cgcccagcag
    12001 gctttccagg cgtccggcaa gatcaagcga ggcatcggac gaactcagct cgatcagcac
    12061 atgaaagggc gaacggctgt ccagtgggca tcgcgtgccc gggatgtgcc tgaggacgaa
    12121 atcgatccct tccgcataca tgaattcata gccggtgagg cactctccgg cgctggcgcg
    12181 gaccaggtcg aacaggtcaa gcccggcctg ccggctgtcg agtccgaccc aggctgtggc
    12241 gcgcaccctt ggcgccggaa agagcttgag gacggcggcg gtgatgattc caagcgtgcc
    12301 ctctgcgccg atgaacaggt ttctcaggtc atagcccgta ttgttcttgc gcagacggtt
    12361 gagatcgttg agcacccgtc catccgccag caccacctca agcccaagcg cgagttcgcg
    12421 tgcattgcca tagcgcagca ccgccgtccc gccggcattg gtggatagat tgccgccgat
    12481 ggtgcacgat ccttcggagc cgagtgaaag tgggaacagg cagccctcag cagtggccgc
    12541 atcctgcacg gttttcagaa tggcgccggc ctcgacggtc atggtgagat tgaccgggtc
    12601 gaccgcgcga atgcggttca tccggtcgag cgacaggacc agtgcgtcgc gcggcacgcc
    12661 gccaccgacc agcccagtgt tgccgccatg ggcgacgaca ggcagacgat ggcggttggc
    12721 cagccagagg catgcggaga catcgtcagt gtcgcgcggt cgggccagcc cgaacgccgt
    12781 gccgcgatag agcccgcgcc cttcaacgag atgcggggcg agatcattgg gatcggtgga
    12841 gatttgcgcc gggccgatcg ttgccgcaag ttcggccaga aagctggaga aggtctcgct
    12901 catgcctcgc accttggccg gcaacccggc cgggcgtcaa ctgccgtgcg ccttacaaga
    12961 gtcctggatc gatgctcggc cgaggccgta accaggccgg gaccgcgagc cccttggatt
    13021 caagaaacgc cggatcgtaa agcttggagg catagcgtgt gcctccgtca cacagcagcg
    13081 tgacgatcgt cttgccgggg ccgagatgcc gcgccagccg gatcgcgcca gcgacattga
    13141 cgccggacga gccgccgacg cacaaccctt ccagcgccag aagatcgaac atcaccggaa
    13201 tggcttcctc atccggaatc tggaagctga catcgaccgg cgcgccctcg atgttgcggg
    13261 tgatgcgccc ctgcccgatg ccttcggtca ccgacgagcc ggcagccttg agttctccgg
    13321 tggtgaagta gctgtgcagc gccgcgccca tcggatcagc cagggcaatg gtgatcgcag
    13381 ggttgcgttc cttaaggccg aggccgatgc cggcgaggga cccgccggtg ccgaccgcac
    13441 agacaaagcc atcaatgcgc tcgtcggtct gttcaaagat ctcgcgcgcc gtggtgcgtc
    13501 ggtgcccctc ccggttggcg gtattgtcga actgattggc ccagaaggcg ccatgcggct
    13561 cgctcctgtt gagctgttcg gccagccggg cggagacctt gatgtagttg ttggggttgg
    13621 catagggcgc ggccggcact tcgacgagat cggcgccggc atggcgcagc atgtccttct
    13681 tctcgcgtga ttgggtctcg ggtatgacga tcaccgtgcg gtgccccagc gcattgccga
    13741 ccagcgccag cccgatgccg gtgttgcctg cggtgccctc gacaatgacg ccgcccggct
    13801 tcagcgcgcc ggaggcacgg ccatcctcaa ggattgccaa tgcggcccgg tccttgaccg
    13861 aaccgccggg attcatgaat tcagccttgc cgagaatggt gcagccggtt gcttcggagg
    13921 cgtggcgcag ccggatcagc ggcgtgttgc caatggcacc gatcaggccg tggcggatgg
    13981 acatggcctt gatcctcagg cgctgggcgg aatgatgaat gcggtcgttg acttgatgcg
    14041 ctccatggca aagcgcgatg tcacgttctt gagcgcgatc gtgttgatca gccgcttgta
    14101 gaactggtca taggcagcca tgtcggcgac caccacatgg gcgagatagt caacatcgcc
    14161 agccatacgg tatagtccca tcacttcggg catggccgag acgatatcgg caaagcgttc
    14221 cagccagggc ccggaatgat ccccggtttc gatagagacg aaaacggtca gccccagccc
    14281 gatcttgtcc ggattgagca gggcaacccg cttggcgata atcccggtgg cttcgagttt
    14341 ctgtatgcgc ttccagcagg gcgtctgcga caacccgacg cgctcggaca attccgccag
    14401 tgactggttg gcgtcctgct gcaacaaggt cagcagtttc cggtcgatca tatccatcat
    14461 ggcggccctt tttcaaaatc gaaaaatttt ttaagcaccg gggatctgtt ctgccagata
    14521 tagcaaaaaa ttcttcgatg caacagttct gcccgtgctt ccactctgtc ttgccaggtg
    14581 cgggatcact ccgcgcggca gggaaagccg cgattcgttc ggattagaaa attattcgtt
    14641 cttcttgtcg aacggttttt catggtttat cccattgttc atctgtttcc gagattgcca
    14701 tgacccgtcc cgaccgccct ccgctggatt gttctgtcct ccacgccgag acacgccttg
    14761 tccatgaggg catattgcgc agcggcttcg gcgagacctc cgaggcgctg tttctcaccc
    14821 agggccatgt ctatgacaat gccgagcaat gtgaggcacg ttttgcgggc gccgatccgg
    14881 ggttcatcta tgcgcgtttt tccaatccga ccgtgcagat gttcgagcgg cgcatggcgg
    14941 gcctggaagg cgccgaggcg gcgcgggcca cggcc
//
LOCUS       DAOMXG010000046        14586 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_2280_length_14586_cov_9.913082, whole genome shotgun sequence.
ACCESSION   DAOMXG010000046 DAOMXG010000000
VERSION     DAOMXG010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 14586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14586
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2280_length_14586_cov_9.913082"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="PLQ11_04825"
     CDS             complement(<1..196)
                     /locus_tag="PLQ11_04825"
                     /inference="COORDINATES: protein motif:HMM:NF014719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HRK24260.1"
                     /translation="MNVSALLLNILGGIALLLWATRLVKTGVFRAFGDGLQRMLHRST
                     ANRLAACGLGVVVATALQSST"
     gene            476..1801
                     /locus_tag="PLQ11_04830"
     CDS             476..1801
                     /locus_tag="PLQ11_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRK24261.1"
                     /translation="MTRRLAMIVAAGLALPTPGHAQAPTGKLTIVTSFSKEVTDPFKA
                     AFEKAYPGTNVDIQNRNTNAGVKFVDETKANNPVDLFWASAPDAFEVLKAKGLLEAYK
                     PKAEGVPEKIGQFPIHDPAGFYSGFAASGYGIMWNTRYVAAQKLPEPKEWQDLATAPY
                     FDHISMAAPSRSGTTHLTLEAILQTAGWDKGWGIIKAFSGNLRSITERSFGVPDAVNS
                     GQVGYGIVIDFFAFSAQGAGFPVKFVYPSLSTIVPANIGVVKNAPNAAAAKAFVDFIL
                     SEPGQAILFDPKIRRLPVRTASYAKAPEGLPNPFEFKWVADAKFDVEKSEKRFAVVDA
                     LYDQLVTFQHDALKAATKAIHEADAAIAKKPNAEAAKLVAEARALIAAMPVTEADAAG
                     PDLAAAFKGAGGKDKAPRQAELEQAWASFAKEKYAAAQAKAEAAVKAAK"
     gene            1821..3539
                     /locus_tag="PLQ11_04835"
     CDS             1821..3539
                     /locus_tag="PLQ11_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRK24262.1"
                     /translation="MTTGTTSPAATRSLGQPGTLVVALLLALFLAAFLVIPVMTVMVA
                     AFQPKGGGGFTLINFVDFFANDLFRRSFWNSVYVAAMSVLLATLFAVPLAYFTTRFDF
                     RGAAIVQTLGIIPLIMPPFAGAVAMQLLFGRNGTINLLLDQAFGFRIPFMEGLNGVIF
                     VEAVHYFPFILINLSAALNAIDRTMEEAAQNLGCSGFRLFRRIVFPLAMPGYVAGASL
                     VFIKVFDDLATPLLLNVKDMLAPQAYLRITSVGLTDPMGYVISVILIVASLVAMYLST
                     LALKGKDYATVQRGGGGLSRRALGPMDALLAYGMITLVLLLVLAPHVGLLLLSLATIW
                     SFSPLPDAYTLAHYARVFSDSGQYIWNTLLYAGLAGLIDVVIGTAIAYLVLRTRTWGA
                     RWLDAVAMAALAVPGVVLGIGFLRTYFGVTLPSGQSLAAFWGMIVLALAIRRLPYALR
                     ASMAGLQQVSASLEEAAQNLGATRGRTVRRIVLPLMTGAIAAGFVTSFATAAVELSAT
                     MMLVQSSNDAPLAYGLYVFMQSPAGRGAGAALGVIAVVIVGISTYISNRIVERDRAGR
                     AGAPGH"
     gene            3564..4631
                     /locus_tag="PLQ11_04840"
     CDS             3564..4631
                     /locus_tag="PLQ11_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRK24263.1"
                     /translation="MSLKAQSAGIEIENVDLSYGQTRVLKDVSLSIRQGEFFAFLGPS
                     GCGKTTLLRLIAGFNTCQSGRVLIGGKDVSALPPWKRDVGMVFQSYALWPHMSVARNV
                     AFGLEERRLPRDQIEDRVDSALDIVGLKHLKDRKPSQLSGGQQQRVALARTIAVRPKV
                     LLLDEPLSNLDARMRVDVRRELRALQQDLKLTTVFVTHDQEEANTISDRIAVMQDGRV
                     QQVGTPQELYDAPANLFVAGFLGTANILAAERQADGRASLPGGVRIDLPDDGRTRAMF
                     RPQNARLATPGDSVSTGRARLTGTISHREFLGSTVRYGVRVGASECLVDAPHHGGEQG
                     FRLGDPVAVDVDRNRILWLAD"
     gene            4696..5334
                     /locus_tag="PLQ11_04845"
     CDS             4696..5334
                     /locus_tag="PLQ11_04845"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRK24264.1"
                     /translation="MDDRYYATARNDVLAHVEGVPARVLEIGCGTGATMQVLRQRGTA
                     FAVGVELDPVSAEAARPQFDRLYVSAVEAAPFEEAIAPGSLDAVFCLDVLEHLVDPWQ
                     VVRRVSPLLKPGGRLHISLPNVRNWKFLWRLFAFGDFHYRDSGVLDRTHLRFFVRSTA
                     VELAECGGLTAIRAVDARVYHPYEIRRLLNLVSFGLTEEWIAKQWIVIAERR"
     gene            5451..6242
                     /gene="iolB"
                     /locus_tag="PLQ11_04850"
     CDS             5451..6242
                     /gene="iolB"
                     /locus_tag="PLQ11_04850"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008124503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HRK24265.1"
                     /translation="MSKLLRHPAATNPVHSVTPRNARWKYVGFEVHDLKRGEVLKAET
                     GNREVCIVMVHGRADVAASGHDFGHIGERSSPFFGEPWAVYVPARSGFRIEADTDCEV
                     ALCSAPAKGTKVARLITPQEVSHETRGNGTNQRFVRNILPDTSPIAESLLVVEVVTPG
                     GHWSSYPPHKHDTDDFPNETYLEETYYHQLKRGSGFAMQRVYTKDGSLDECMAVGHRD
                     VVLVPRGYHPVGAPHGFDLWYLNVMAGPKRAWKFSFDHDHSFLTW"
     gene            6407..7993
                     /locus_tag="PLQ11_04855"
     CDS             6407..7993
                     /locus_tag="PLQ11_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRK24266.1"
                     /translation="MSIRKLCLATVAALALASVSANAVTLRYANQGDLKSLDPYTLNE
                     TTANAHLGHVYEGLTKRDRELKIIPGLAERWEVMDGAKKWRFYLRKGVKFQNGEDFTA
                     DDVIFSADRVRAQGSNFQTRVPKGAKFSKIDDHTVEVELESPNPIMNAQWDTWYIMSK
                     KWAEANNTAAPTPASATSPSHASLNANGTGPFRIESHQPGVKTVFKPHAGWWGKKEHN
                     LDEIVFTPISNDATRVAALLSGEVDVIEPVPIQDIPRVNSSDKARVLAGPESRTIFLG
                     FDQIRDELLESNVKGKNPFKDVRVRQAFNMAIDKETIKTRVMRGLSTPSPLMIAPELV
                     PWPKDFQNPKFDPDAAKKLLEEAGYPNGFEVGMDCPNDRYVNDGPICQAVVGMLARIG
                     VKVNLNAQPKAQYFAKILKPGGYKTSFYLLGWTPGTFDSHNVLYDIHGCRAEGSPRGE
                     SNVGGYCNKTVDELADKVLVESDTAKRNALIKEAFQITTKEFAYVPLHQQALAWGVAK
                     NVELSQRPDNSVLLYWATKK"
     gene            8061..9038
                     /locus_tag="PLQ11_04860"
     CDS             8061..9038
                     /locus_tag="PLQ11_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRK24267.1"
                     /translation="MLAFILRRIAQSLLVMVTVALIAFVMFRFVGDPVNQMVGVDTPQ
                     AEVARLRESLGLNDSTIVQFGRFLWNALHFEFGTSYQFRLPVTQLLGERAPATLELAL
                     VASLFALIVGVVMGVYCALRPNGILARLFQAISLVGVSLPTFLIGILLIYVFAVTLGW
                     LPSNGRGQVVKLGRWWTTGFFTASGLRALILPAITLGLFQMTLIMRLVRAEMMEVMRT
                     DYVKFARARGIRTRSIHFRHALKNTLIPVITIAGLQLGSIIAFAIITETVFNWPGMGL
                     LFLKAVQNVDIPIMAAYLMLIGLLFVTINLIVDLAYGLVDPRLRVAMRS"
     gene            9042..10004
                     /locus_tag="PLQ11_04865"
     CDS             9042..10004
                     /locus_tag="PLQ11_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003499545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRK24268.1"
                     /translation="MPTPTPEEVKGLVASGGATLASRIRDSDIYLSFRASPLTMLAAA
                     IVVIMVAGALLAAWIAPTNPFDPATVFLADASIPPRWQQGGDPRFVIGTDDQGRDLYS
                     AILYGLRVSLLVGGMGVLLAAVIGITLGLVAGYFGGRVDAIIMRIADVQLTFPAILIA
                     LLIDGVVQALMKGVPREQSAIPVLVISIGLSFWVQYARTIRGSTMVEKNKDYVAAARL
                     VGLPAPVIMLRHVLPNVIGPVLVILTINLGLAIITEATLSFLGVGLPPTQPSLGTLIA
                     IGNKYLFSGDWWIVTFPGLTLAILVLAVNLLGDWLRDALNPKLR"
     gene            10008..11021
                     /locus_tag="PLQ11_04870"
     CDS             10008..11021
                     /locus_tag="PLQ11_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRK24269.1"
                     /translation="MPEPVVSVRNLKVEFAGRRGTLVAIDDVSFDIHRGEVLGVVGES
                     GAGKSVTGSAIIGLIEPPGRIAGGQILLHGQRIDNLAPEEMRRLRGRKIAMIFQDPLT
                     SLNPLFTVGEQLVETILTHLDLSPAEARAISLLGEVGIPAPDKRIDGYPHEFSGGMRQ
                     RVVIALALSAEPDFIVADEPTTALDVSVQAQIIALLKKLCRERGTAVMLVTHDMGVIA
                     ETADRVAVMYAGRVAEIGPVRDVVKHPLHPYARGLMGAIPTLESEADRLVQIPGSMPR
                     LTNIPRGCAFNPRCDRLIDRCRQDRPDPIEPDPATAAREHLMAGHRVACHLYEPRKTG
                     AKQ"
     gene            11018..12010
                     /locus_tag="PLQ11_04875"
     CDS             11018..12010
                     /locus_tag="PLQ11_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012321397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRK24270.1"
                     /translation="MNAPAPLVSVRDLRRVFDVSRPWLNRVLEGSEKQFLKAVDGVTF
                     DIRKGETFALVGESGSGKSTVARMAVGLLPMSGGSVTIDGVAMGTGEDSRERRALRKR
                     IQMIFQDPYASLNPRWKVERIIAEPIRAFGLAQGADLVARIGELLTLVGLHPDDRTKF
                     PHEFSGGQRQRIAIARALAANAEFIVCDEPTSALDVSVQAQILNLMRDLQQRLGLTYL
                     FISHNLAVVRHMADRIGVMYLGRLVEVSEGKRLFSAPRHPYTRMLLDAVPDLGMTGRQ
                     RIAVAGEIPNPINPPSGCTFHPRCPLASDRCKREAPQPLDGVACHAVEEGRSGR"
     gene            complement(12020..13186)
                     /locus_tag="PLQ11_04880"
     CDS             complement(12020..13186)
                     /locus_tag="PLQ11_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004107746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HRK24271.1"
                     /translation="MAIDSRVLDLAGTVRGWRQQLHQQPELGYEVHRTAAFVAEKLRA
                     FGCDEVVEGIGRTGVVGIVHGRKGAGGRVVGLRADMDALPIEEETNLPYRSKVAGVMH
                     ACGHDGHTAMLLGAARHLTATREFDGTVALIFQPAEEGGAGGKAMVEDGMMDRWSIAE
                     VYGMHNMPGQPVGSFGARSGPIMAAADEFVITVEGKGGHAAAPHLGVDPVIVGVHIIS
                     ALQTIASRNADPLHSVVVSTTMFHAGTAFNVIPTRAELTGTVRTLNASIRSMAEARLK
                     EIAIGTARVFGANATVSYRYGYPATVNHERETILATEIARGIAGPENVDGNIAPMMGG
                     EDFSYMLEARPGAYLFIGNGDTAGLHNAAYDFNDEVLPYGMQFFINVARERTAR"
     gene            13277..14458
                     /locus_tag="PLQ11_04885"
     CDS             13277..14458
                     /locus_tag="PLQ11_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019906234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HRK24272.1"
                     /translation="MPACTGACPLKQSSSWTRQTLAAQALGRIDAATKAVVTPIHVAT
                     TFIRDPDNQYRSGFEYGRADNGSVREAEAIIAALEGANTAMVLGSGMSAATAVFLALK
                     PGDHVIAPRVMYWGLRNWLLTYATEWGLQIELVDTGDTAAVAAALRPGKTKLVWLETP
                     ANPLWTICDIAAISAMAKKAGARVAVDSTCATPLLTRPLEHGADIVMHSATKYLNGHS
                     DVIAGALVTGAEDELWSRIKTLRGKLGLILGGFEAFLLIRGMRTLPLRLRAACDGAME
                     LARRFSNHPGVAEVLYPGLESHPGHAIARRQMQGGFGGMLSIRIRGGEAAAIATAARV
                     DLWKRATSLGGVESLIEHRASVEGAGSPCPPDLLRLSVGAEDIEDLFADLDRALRAAT
                     G"
BASE COUNT         2430 a         4727 c         4662 g         2767 t
ORIGIN      
        1 ccgtcgaact ctgcagcgcc gtcgccacca ccacgccaag gccgcaggcc gccagccgat
       61 tcgccgtcga gcggtgcagc atgcgttgca agccatcgcc gaaggcccgg aaaacgccgg
      121 tcttgaccag tcgcgtcgcc cacagaagca gggcgattcc gcccaggata ttcagcagga
      181 gagccgaaac gttcatcgca accgagatgc tccccgaaac agttcgcacc ggcgtcagcc
      241 ggaccatttg ccgttgcaga accgtcgact cgttggtcgc cgcaccgctt ttttgttagc
      301 cgactgaacc ggcttttgca tcccgcgtca atgcgttgtg cgaaaggcgt gggccagccg
      361 gttcaagcag gggcctgact tgcgccctgt ccgcgctgct gtgagttgac gagcgcggca
      421 ttccttcgca ctctgccgcc aatcaaacga acgagatagg gagcacccga tgcctctgac
      481 ccgccgcctt gccatgatcg tcgccgccgg ccttgccctg cccacacctg gccatgcgca
      541 ggccccgacc ggcaagctca ccatcgtcac ctcgttctca aaggaagtga ccgatccttt
      601 caaggcggcg ttcgagaaag cctatcccgg caccaatgtc gatatccaga accgcaacac
      661 caatgcgggg gtgaaattcg tcgacgagac caaggccaac aacccggtcg acctgttctg
      721 ggcatcggca cccgatgcgt tcgaggtgct caaggccaag ggcctgctcg aagcctacaa
      781 gcccaaagcc gaaggcgttc cggaaaagat cggccagttt ccgatccatg atccggctgg
      841 cttctattcg ggctttgccg cgtcgggcta cggaatcatg tggaacaccc gctatgtagc
      901 ggcacagaag ctgcctgagc cgaaggaatg gcaggatctg gccaccgcgc cctatttcga
      961 ccacatctcg atggcggcgc cttctcgctc cggcaccacc catctgacgc tggaagccat
     1021 tctgcagacc gccggctggg acaagggatg gggcatcatc aaggccttct cgggcaatct
     1081 gcgctcgatc accgagcgct cgttcggcgt gccggatgcg gtcaactccg gtcaggtcgg
     1141 ctacgggatc gtcatcgatt tctttgcctt ctccgcccag ggcgcgggct ttccggtcaa
     1201 gttcgtctac ccctcgctgt cgaccatcgt gccggccaat atcggcgtgg tgaagaacgc
     1261 gccgaacgcc gccgccgcca aggcgtttgt cgatttcatc ctgtccgagc cgggtcaggc
     1321 gatcctgttc gatccgaaga tccgccgcct gccggtgaga acggcaagct acgccaaggc
     1381 gccggaaggg ctgcccaacc cgttcgagtt caaatgggtc gccgatgcca aattcgatgt
     1441 cgagaagtcg gaaaagcgct ttgccgtggt cgatgcactc tacgatcagc ttgtgacctt
     1501 ccagcacgac gcgctgaagg ctgcgaccaa ggccattcac gaagccgatg cggcaatcgc
     1561 caaaaagccc aatgcggagg ccgcgaaact ggtggccgaa gcccgtgcgc tgatcgccgc
     1621 catgccggtc accgaagcgg atgcggccgg tcccgatctg gcagcggcgt tcaagggcgc
     1681 cggcggcaag gacaaggcgc cgcgtcaggc cgagcttgaa caggcctggg ccagctttgc
     1741 caaggagaaa tatgcggcgg cgcaggccaa ggccgaggcg gccgtgaagg cggccaagtg
     1801 acggctgcga gcgcactctg atgactaccg ggaccacatc gccggccgcc acgcggagcc
     1861 ttggccaacc aggcaccctg gtcgttgccc tgttgctggc gctgtttctc gcggcgttcc
     1921 tggtcattcc ggtgatgacg gtgatggttg ccgccttcca gcccaagggc ggcggcggct
     1981 tcacgctgat caacttcgtc gatttctttg ccaacgatct gttccgccgc tcgttctgga
     2041 actcggtcta tgtcgcggcg atgtcggtct tgctggcgac gctgtttgcg gtgccgcttg
     2101 cctatttcac cacgcggttc gacttccgcg gcgccgccat cgtgcagaca ctcggcatca
     2161 ttcccttgat catgccgcca tttgccggag cggtggcgat gcagctgctg ttcgggcgca
     2221 atggcacgat caacctgctg ctcgatcagg cgttcggctt ccgcattccg ttcatggaag
     2281 ggctcaacgg cgtcattttc gtcgaggcgg tgcattattt tccgttcatc ctgatcaatc
     2341 tgtcggcggc gttgaacgcc atcgaccgga cgatggagga agcggcgcag aatctcggct
     2401 gttccgggtt ccggctgttc cgccgcatcg tgtttccgct ggcgatgccc ggctatgtcg
     2461 ccggcgcgag tctcgtgttc atcaaggtgt tcgacgatct ggcgacgccg ctgctgctca
     2521 acgtcaagga catgctggcg ccgcaggcct atctgcgcat cacctcggtc gggctgaccg
     2581 atccgatggg ctatgtgatc tcggtgatcc tgatcgtcgc ctcgctggtg gcgatgtatc
     2641 tgtcgaccct ggcgctcaag ggcaaggatt acgccacggt ccagcggggc ggcggcgggc
     2701 tgtcgcggcg ggcgctgggc cccatggacg cgctgctcgc ctatggcatg atcacccttg
     2761 tgctgctgct ggtgctggcg ccgcatgtcg gcctgctgct gctgtcgctg gcgaccatct
     2821 ggtcgttctc gccgctgccc gatgcctata cgctggccca ctacgcccgg gtgttttccg
     2881 acagtggcca gtacatctgg aacacgctgc tctatgccgg gctggccgga ttgatcgacg
     2941 tggtgatcgg cacggccatc gcctatctgg tgctgcgcac ccgcacctgg ggagcccggt
     3001 ggctcgatgc tgtcgccatg gcggcgctgg cggttcccgg ggttgtgctg ggcatcggct
     3061 tcctgcgcac ctatttcggc gtcaccttgc cgtccggcca gtcgctggcc gccttctggg
     3121 gcatgatcgt gctggccctg gcgatccgcc gcctgccgta tgctctgcgg gcgtcgatgg
     3181 cggggttgca gcaggtgtcc gcatcgctgg aggaggcggc gcagaatctc ggcgcaacgc
     3241 gcgggcgcac cgtgcgccgc atcgtgctgc cgctgatgac cggggcgatc gccgccggct
     3301 ttgtcacctc ctttgcgacg gcggcggtcg agctctcggc cacgatgatg ctggtgcagt
     3361 cgagcaacga tgcgccgctc gcttatgggc tttacgtgtt catgcaatcg ccggcggggc
     3421 gcggcgcggg cgcggcgctt ggcgtcatcg cggtggtgat cgtcggcatt tccacctaca
     3481 tttccaaccg catcgtcgag cgtgaccggg ccggcagggc cggcgcgccc gggcactgac
     3541 ctgaccgacc ggagatccgc acgatgagcc tcaaagccca gtccgccggc atcgagatcg
     3601 agaacgttga cctgtcctac ggccagaccc gggtgctgaa ggatgtcagc ctttcgatcc
     3661 ggcagggcga gttctttgcc ttcctcggcc cctcgggctg cggcaagacc acgctgctgc
     3721 ggctgatcgc cgggttcaac acctgccaga gcggccgcgt gctgatcggc gggaaggatg
     3781 tctccgccct gcccccgtgg aagcgcgacg tcggcatggt gttccagtcc tatgcgctgt
     3841 ggccgcatat gagcgttgcc cgcaatgttg ccttcgggct ggaggagcgg cgcctgccgc
     3901 gtgaccagat cgaggatcgg gtcgattcgg cgctggatat cgtcgggctg aagcacctca
     3961 aggatcgcaa gccctcccag ctttccggcg gccagcagca gcgtgtcgcg ctcgcccgca
     4021 ccatcgccgt gcggccgaaa gtgctgctgc tcgacgagcc gctgtccaat ctcgacgcca
     4081 gaatgcgcgt cgatgtacgg cgtgaattgc gtgcgctgca gcaggacctg aagctgacca
     4141 ccgtcttcgt cacccacgat caggaagagg ccaacaccat ttccgaccgg atcgcggtga
     4201 tgcaggatgg ccgcgtgcag caggtcggaa ccccgcagga gctttatgac gcgccggcca
     4261 acctgttcgt cgccgggttt ctgggaaccg ccaacatcct tgccgccgag cggcaggccg
     4321 acggtcgggc gtcgctgccg ggcggcgtgc ggatcgatct gcctgatgac gggcgcacgc
     4381 gggccatgtt ccgtccgcag aacgctcggc tggcgacgcc gggcgactct gtgtcgaccg
     4441 ggcgggcgcg actgaccgga accatcagcc atcgcgaatt cctcggctcg acggtgcgat
     4501 atggcgttcg ggtcggcgcc agcgaatgcc ttgtcgatgc tccccaccac ggcggtgaac
     4561 aggggttccg tctcggcgat ccggtggctg tcgatgtcga tcgcaaccgc atcctctggc
     4621 tcgccgactg atcgggctgg ccatgccagg ccgttgatgc tacctcgcgc ggcgtccaca
     4681 tccgggggaa ggcttttgga cgaccgctac tacgccactg cccgcaacga tgtcctcgcc
     4741 catgtcgagg gcgttccggc gcgcgtgctc gagatcggtt gcggcaccgg cgcgacgatg
     4801 caggtgctgc gccagcgcgg aacggctttt gccgtgggcg tcgaactcga tccggtttcg
     4861 gccgaggcgg cacggccgca gttcgaccgg ctctatgttt cggcggtgga agcggccccg
     4921 ttcgaggagg cgatcgcgcc gggttcgctc gatgcggtgt tctgcctcga tgtgctcgaa
     4981 catctggtcg atccctggca ggtggtgcgg cgggtctcgc cgctgctcaa gccgggcggg
     5041 cggctccata tctcgctgcc caacgtccgc aactggaagt ttctctggcg gctgttcgcc
     5101 ttcggcgatt ttcactatcg cgattcgggc gtgctggatc gcacccatct gcggttcttc
     5161 gtgcggtcga cggcagtcga actggccgaa tgcggcggtt tgaccgcgat ccgggcggtg
     5221 gatgcgcggg tctatcatcc ttacgagatc cgccggctgc tcaatctcgt cagcttcggc
     5281 ctgacagagg aatggatcgc caaacaatgg atcgtcattg ccgaacggcg gtgagacctc
     5341 ctgccgcagg cggacgcgga cgcagggcga ctggtccgaa tcccgatgtt gggctacgtc
     5401 ttgatccgac gagacggtcg gcgcgcagac gccggcagag gggagacgcc atgtccaagc
     5461 tgttgcggca tccggccgcc accaatcctg tccacagcgt cacgccacgc aatgcgcgct
     5521 ggaaatatgt cggcttcgag gtgcatgacc tcaagcgcgg cgaggtgctg aaagccgaga
     5581 ccggcaatcg cgaggtctgc atcgtcatgg tccatggccg ggccgatgtc gcagcctccg
     5641 gccatgattt cggccatatc ggcgagcgat cgagcccgtt cttcggcgag ccctgggccg
     5701 tctatgtgcc ggccagatcc ggtttccgca tcgaggcgga taccgattgc gaagtggcgc
     5761 tgtgttcggc cccggccaag ggcaccaagg tagcccggct catcaccccg caggaagtca
     5821 gccacgagac gcgtggcaat ggcacaaacc agcggtttgt ccgcaatatc ctgcccgata
     5881 cgtccccgat cgccgagagc ctgctggtgg tcgaagtggt gacccccggc ggccactggt
     5941 cgtcctatcc gccgcacaag catgacaccg acgatttccc gaacgagacc tatctggagg
     6001 aaacctacta ccatcagctc aagcgcggct cgggctttgc catgcagcgc gtctacacca
     6061 aggatggcag cctggatgag tgcatggctg tcggccaccg cgatgtcgtg ctggtgccca
     6121 ggggctatca tccggtcggc gcgccgcacg gctttgacct gtggtatctc aacgtgatgg
     6181 ccggaccgaa gcgggcgtgg aaattctcgt tcgaccacga ccacagcttc ctgacctggt
     6241 gaccggccat cgccagcagc tcaggtcgtc attgactaag gtctcatagg ggcgctaggg
     6301 tcgctccaaa cagggcgctg caccgggagg gtttttgctg tccgcacctg tttctggttg
     6361 ttcttgaaga acatacaatg gtctcttcaa cgggagaatg aagaaaatgt cgatccgaaa
     6421 actgtgcctc gccactgttg cggcgctggc tctggcatct gtgtccgcca atgccgtcac
     6481 gctgcgttat gccaaccagg gcgatctgaa gtcgcttgac ccctatacct tgaacgaaac
     6541 caccgccaac gcccatctcg gccatgtcta cgaggggctg accaagcgcg accgcgagct
     6601 gaagatcatt ccgggtctgg cggagcgctg ggaggttatg gacggcgcca agaagtggcg
     6661 gttctatctg cgcaagggcg tcaagttcca gaatggcgag gatttcaccg ctgatgacgt
     6721 gatcttctcc gccgatcggg tgcgggctca gggctcgaac ttccagacgc gcgtgcccaa
     6781 gggcgccaag ttcagcaaga tcgatgacca caccgtcgag gtcgagctgg aatcgcccaa
     6841 cccgatcatg aatgcccagt gggatacctg gtatatcatg tcgaagaagt gggcggaggc
     6901 gaacaacacc gccgcgccga ctccggcctc ggccacttcg cccagccatg cctcgctgaa
     6961 tgccaacggc accggtccgt tcaggatcga gagccatcag ccgggcgtca agaccgtgtt
     7021 caagccgcat gccggctggt ggggcaagaa ggagcacaat ctcgacgaga tcgtgttcac
     7081 cccgatctcc aacgacgcaa cccgcgtcgc ggcgctgctg tcgggcgagg ttgacgtgat
     7141 cgagccggtg ccgatccagg acattccgcg cgtcaattcg tcggacaagg cacgtgtgct
     7201 ggccgggccg gaatcgcgca cgatcttcct cggcttcgac cagatccgtg atgaactgct
     7261 ggaatcgaac gtcaagggca agaacccgtt caaggatgtg cgggtgcgcc aggcgttcaa
     7321 catggcgatt gacaaggaga cgatcaagac gcgcgtcatg cgcggtctgt cgaccccctc
     7381 gccgctgatg atcgcgccgg aactggttcc gtggccgaag gacttccaga atccgaagtt
     7441 cgatccggac gcggccaaga agctgctgga ggaagccggc tacccgaacg ggttcgaggt
     7501 cggcatggat tgcccgaacg accgctatgt caacgacggc ccgatctgcc aggcggttgt
     7561 cggcatgctg gcgcgcatcg gcgtcaaggt gaacctcaac gcccagccga aggcgcagta
     7621 tttcgccaag atcctcaagc ccggcggtta caagacctcg ttctatctgc tcggctggac
     7681 ccctggcacc ttcgacagcc acaacgtgct ctatgacatc catggctgcc gggccgaagg
     7741 ctcgccgcgc ggggagtcga atgtcggtgg ttattgcaac aagaccgtcg atgaactcgc
     7801 cgacaaggtt ctggtggaaa gcgataccgc caagcgcaac gctctgatca aggaagcgtt
     7861 ccagatcacc accaaggagt tcgcctacgt tccgctgcac cagcaggcgc ttgcctgggg
     7921 tgtcgccaag aacgtcgagc tctcgcagcg cccggacaat tcggtgctgc tctactgggc
     7981 caccaagaag taactcccga gaacgccgtg acgggaaggc ccccttcccg tccggccctt
     8041 ggtgccagcc ggttttcctg atgcttgctt tcatcctccg gcgtattgcg cagtcgcttc
     8101 tcgtcatggt gacggtagcg ctgatcgcct tcgtgatgtt ccgttttgtc ggcgacccgg
     8161 tcaaccagat ggtcggggtc gatacgccgc aggcggaggt cgcgcgcctg cgcgagagtc
     8221 ttgggctgaa tgattcgacc atcgtgcagt tcggccggtt cctgtggaac gcgctgcatt
     8281 tcgaattcgg cacttcctac cagttccgcc tgccggtgac gcagctgctg ggcgagcggg
     8341 cccctgcgac gctcgaactg gcgctggtcg cctcgctgtt tgcgctcatt gtcggcgtcg
     8401 tcatgggcgt ttattgtgcg ctgcggccga atggcattct ggcgcggctg tttcaggcga
     8461 tcagtctcgt cggcgtgagc ctgccgacct ttctgatcgg catcctgctg atctacgtat
     8521 tcgcggtgac gctcggctgg ctgccgtcga acgggcgcgg ccaggtggtc aagctcggcc
     8581 gctggtggac gaccggcttt ttcaccgcct ccggcctaag ggccttgatc cttccggcaa
     8641 tcaccctggg ccttttccag atgaccctga tcatgcgcct tgtgcgcgcc gagatgatgg
     8701 aggtcatgcg caccgattat gtgaaattcg cccgtgcgcg cggcatacgc acccggtcga
     8761 ttcacttccg ccatgcgctg aagaacacgc tgatcccggt gattaccatc gccgggctgc
     8821 aacttggctc gatcatcgcc tttgccatca tcaccgagac ggtattcaac tggccgggca
     8881 tgggcctgtt gttcctcaag gcggtgcaga atgtcgatat tccgatcatg gcggcctatc
     8941 tgatgctgat cgggctgttg ttcgtgacca tcaacctgat cgtcgatctg gcctatggcc
     9001 ttgtcgatcc gcgcctgcgc gtggcgatgc ggagctgaac gatgccgaca cccacgcccg
     9061 aggaggtcaa ggggcttgtc gccagcggcg gcgcaacgct cgcgtcgcgg atcagggact
     9121 cggacatcta tctcagcttc cgcgcctcgc cgctgacgat gctggctgca gcaatcgtcg
     9181 tcatcatggt cgccggggcc ctgctggcag cgtggatagc cccgaccaat ccgttcgatc
     9241 cggcgacggt gtttctcgcc gatgcctcga tcccgccgcg ctggcagcag ggcggcgatc
     9301 cgcgctttgt catcggcacc gatgatcagg gccgcgatct ttattcggcc attctctacg
     9361 ggctgcgggt ttcgctgctg gtcggcggga tgggtgtgct gctggcggcg gtcatcggca
     9421 tcacgttggg gctggtggcc ggctatttcg gcggccgggt tgatgcgatc atcatgcgca
     9481 tcgccgatgt gcagctgacc ttcccggcca tcctgatcgc tctgctgatc gatggcgtgg
     9541 tgcaggcgct gatgaagggc gtgccgcgcg agcaatcagc gattccggtg ctggtcattt
     9601 ccattggcct gtccttctgg gtgcagtatg cgcgcaccat ccgcggctcg accatggtgg
     9661 agaagaacaa ggattatgtt gccgccgccc ggctggtcgg cctgccggcg ccggtgatca
     9721 tgctgcggca tgtgctgccg aacgtgatcg gcccggttct ggtgatcctg acgatcaacc
     9781 tcggcctggc gatcatcacc gaggcgacgc tgtcgtttct cggcgtcggc ctgccgccga
     9841 cccagccctc gctcggcacg ctgatcgcca tcggcaacaa atacctgttt tccggcgact
     9901 ggtggattgt caccttcccc gggctgacgc tggccatcct ggtgctggcg gtgaacctgc
     9961 tgggcgactg gctgcgcgac gcgctcaacc cgaagctgag gtgaggcgtg cccgaaccgg
    10021 tcgtttccgt tcgcaatctg aaggtcgaat ttgccggccg gcgcggcacg ctggtcgcca
    10081 tcgacgatgt gtcgttcgac atccatcgcg gcgaggtgct gggcgtggtc ggggaatccg
    10141 gcgccggcaa atcggtgacc ggatcggcga tcatcggcct gatcgagccg cccggccgca
    10201 tcgccggcgg ccagatcctg ctgcatggac aaaggatcga caatcttgcc ccggaggaga
    10261 tgcggcggct gcggggacgc aagatcgcca tgatcttcca ggacccgctg acctcgctca
    10321 atcccctgtt cacggtgggc gagcaactgg tcgagaccat tctgacccat ctcgacctgt
    10381 cgccggccga ggcgcgggcc atcagcctct tgggcgaggt cggcattccc gcgcccgaca
    10441 agcgcatcga cggctatccg cacgagtttt ccggcggcat gcgccagcgt gtcgtcatcg
    10501 cactggcatt gtcggccgag cccgatttca tcgttgccga cgagccgacc accgcgctcg
    10561 atgtttccgt gcaggcgcag atcatcgccc ttctgaaaaa gctctgccgc gagcgaggta
    10621 cggcggtgat gctggtgacc cacgacatgg gcgtgatcgc cgaaaccgcc gatcgcgtgg
    10681 cggtgatgta tgcagggcgt gtcgccgaga tcggcccggt gcgcgatgtg gtcaagcatc
    10741 cgctgcaccc ctacgccagg gggctgatgg gcgcgatccc gacgctggaa agcgaggcgg
    10801 accggctggt gcagatcccc ggctcgatgc cgcgcctgac caacatcccc agaggatgcg
    10861 ccttcaatcc gcgctgcgac cggctgattg accgctgccg ccaggatcgg cccgatccga
    10921 tcgagcccga tcccgccacc gccgcccgcg agcacctgat ggccggccat cgcgttgcct
    10981 gccacctcta cgagccccgc aagaccggag cgaagcaatg aacgctcccg cgcccctcgt
    11041 atccgtgcgc gacctcaggc gagtgttcga cgtttcgcgc ccctggctca accgcgtgct
    11101 ggaaggcagc gagaagcagt ttctcaaggc ggtggacggc gtcaccttcg acatccgcaa
    11161 gggcgagacc ttcgcgctgg ttggcgaatc gggctctggc aaatccacgg tggcgcggat
    11221 ggcggtggga ttgctgccga tgagcggcgg atccgtcacc atcgacggcg tggcgatggg
    11281 aaccggcgag gacagcaggg aacgccgcgc cctacgcaag cgcatccaga tgatctttca
    11341 ggatccctat gccagcctca atccccgctg gaaggtcgaa cgcatcattg ccgagccgat
    11401 ccgggcgttc ggactggcgc aaggcgccga ccttgtcgcc cggatcggtg aactgctgac
    11461 gctggtcggc ctgcatccgg atgaccggac gaaatttccg cacgaatttt ccggcggcca
    11521 gcgccagcgc atcgccatcg cccgagcttt ggcggccaat gccgaattca tcgtctgcga
    11581 cgaaccgacc tcggcgctgg atgtctcggt tcaggcgcag atcctcaatc tgatgcgcga
    11641 tctccagcag cgcctggggc tcacctatct gttcatttcg cacaatctcg ccgtggtccg
    11701 gcacatggcc gaccgcatcg gcgtgatgta tcttggccgg ctggttgagg tgagtgaggg
    11761 caagcggctg ttttcggccc cgcgccatcc ctatacgcgc atgctgcttg atgcggttcc
    11821 cgatctcggc atgaccggcc ggcagcgcat tgcggtcgcc ggagaaattc ccaacccgat
    11881 caatccgccc tcaggatgca ctttccaccc gcgttgtccg ctcgccagcg accgctgcaa
    11941 gcgtgaggcg ccgcagccgc tggacggtgt ggcctgccat gccgtcgagg aaggacgcag
    12001 cggccgatag gcaaaacggt cagcgcgctg tccgctcccg ggcgacattg atgaagaact
    12061 gcatgccgta gggcagcacc tcatcgttga aatcataggc ggcattgtgc aacccggccg
    12121 tatcgccatt gccgatgaac agataggcgc cgggccgtgc ttccagcatg taggagaaat
    12181 cctccccgcc catcatcggc gcgatattcc cgtcgacatt ctcggggccg gcaattccgc
    12241 gtgcgatctc ggtcgccaga atcgtctcgc gctcatggtt gacagtcgcc ggatagccgt
    12301 agcggtagct caccgtcgca ttcgcgccga aaacgcgggc ggtgccgatc gcgatctcct
    12361 tcagccgcgc ctccgccatc gaccggatgc tggcattgag cgttcgcacc gtaccggtga
    12421 gttcggcgcg ggtcgggatc acgttgaagg ccgtgccggc atggaacatc gtcgttgaga
    12481 cgacgaccga atgcagcggg tcggcattgc gcgaggcgat ggtctgcaag gcgctgatga
    12541 tatgcacgcc aacgatcacc ggatcgaccc cgagatgcgg ggccgccgca tgcccgccct
    12601 tgccctcgac ggtgatgacg aattcatcgg cagcagccat gatcggcccg gagcgggccc
    12661 cgaagctgcc gaccggctgt ccgggcatgt tgtgcatgcc gtagacctcg gcgatcgacc
    12721 agcggtccat catgccatcc tcgaccatcg ccttgccgcc ggcgccgcct tcctccgccg
    12781 gctggaagat cagcgcgacg gtgccgtcga attcgcgcgt ggcggtcagg tggcgggccg
    12841 caccgagcag catggcggta tggccgtcat ggccgcaggc atgcatgacg ccggcaacct
    12901 tcgaccggta gggcagattg gtctcttcct cgatgggcag cgcgtccata tccgcccgca
    12961 ggccgacgac ccggccgccc gcccccttgc gcccatggac gatcccgacg acgcctgtcc
    13021 ggccgatgcc ttcgaccacc tcgtcgcagc cgaaggcgcg cagcttctcg gccacgaagg
    13081 ccgcagtccg gtgcacctca tagccaagct cgggctgctg atgcaattgc tggcgccatc
    13141 cccggaccgt gccggccagg tcaaggacgc gtgaatcgat agccatgggt ccctctcagc
    13201 aaaccttgat ctttgaaatc cattgcgggg ctgtcaaccc aaaagcatgc ttgcacacga
    13261 agctttgttg gccacaatgc cggcctgcac aggagcatgc cccttgaaac agtcatcctc
    13321 atggacccgc cagacccttg ccgcccaggc cctcggccgc atcgacgcgg cgaccaaggc
    13381 cgtcgtcacg ccgatccatg tggccaccac cttcatccgc gatcctgaca atcagtatcg
    13441 ttccggtttt gaatatggtc gtgccgacaa tggctcggtg cgggaggcgg aggccatcat
    13501 cgcggcgctg gaaggggcca acactgccat ggtgcttggc tcgggcatgt ccgccgcaac
    13561 ggcggttttt ctcgcgctga aacccggcga tcatgtgatc gcgcccaggg tgatgtattg
    13621 gggcctgcgc aactggctgc ttacctatgc caccgaatgg ggcttgcaga ttgaattggt
    13681 cgatacgggc gatacggccg ccgtcgctgc cgcccttcgc cccggcaaga ccaagcttgt
    13741 ctggctggaa acgccagcca atccattgtg gacgatctgt gacatcgcgg cgatcagcgc
    13801 gatggcgaag aaggccggcg cgcgcgtcgc cgtcgattca acctgcgcga cgccgctgct
    13861 gacccgtccg ctggagcatg gcgccgatat cgtgatgcat tcggccacaa aatatctcaa
    13921 tggccattcc gatgtgatcg ccggagcgct ggtgaccggc gccgaagacg aattgtggtc
    13981 acgcatcaag accttgcgtg gcaagcttgg gctgatcctc ggcgggttcg aagccttcct
    14041 gctcattcgc ggcatgcgca ccctcccgct gcggctgcgg gccgcctgcg acggcgcgat
    14101 ggaactggcg cggcgctttt ccaatcaccc cggcgtggcc gaggtgctct atcccgggct
    14161 ggaaagccat ccgggccatg ccatcgcccg gcggcagatg cagggcggct tcggcggcat
    14221 gctgtcgatc cgaatccggg gcggcgaggc agcggccatc gccaccgctg cccgggtgga
    14281 cttatggaag cgggcgacct cgctgggcgg cgtcgaaagc ctgatcgagc atcgcgcctc
    14341 ggtcgagggc gcgggcagtc cctgcccgcc agacctgctg cgcctgtcgg tgggcgccga
    14401 ggatatcgag gacctgttcg ccgatctcga ccgcgcctta agggccgcaa caggctgatc
    14461 gaaaagcgct ctatattttt gtttttcgcc ttttcttgac gcgaaccgga agccacttcg
    14521 ctcgaaaatg ctctaggctc aggactcatt gattgagata gaaagcgacg gcggccgcga
    14581 tgcaga
//
LOCUS       DAOMXG010000047        14574 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_2284_length_14574_cov_9.582823, whole genome shotgun sequence.
ACCESSION   DAOMXG010000047 DAOMXG010000000
VERSION     DAOMXG010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 14574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14574
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2284_length_14574_cov_9.582823"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..1465
                     /locus_tag="PLQ11_04890"
     CDS             125..1465
                     /locus_tag="PLQ11_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRK24273.1"
                     /translation="MPVAGPKLRADFVRRNRCGRFCLAVMTLLLAVGLAAAQAPPAGE
                     ATPQGELRQVLEKIQRSEAEQSRLRSEITGLKGDRAQLNKDLLASTVAVREAEVRIGE
                     AQARLNEVTASEQALKQSLEARRGVITEVLATLQRMGRRSPPAVLVRPEDMLEAIRSA
                     ILLGHVVPDIKAEAEQLAADLSELVRLRRAAAAERQRLQGERDRIEAERTRLAGLIDA
                     RQSQIESSEKALGDERGRLDGMARQAQTLKDLIARLEQDAASMQRVQEQAKKAPSPAQ
                     SQEDATRLAALAFRDPARLAPKIAFQDARGLLALPTNGPVIKVFGAADGFGGSERGIT
                     IAAQPGHVVTAPSDGWILFAGPYRSYGRILIINAGNGYNFVLTGLQSTSVEPGQFVLA
                     GEPVGMMGSPALEAARTGDGQPATSHLYVELRKDNQPIDPTPWWAKATNEKARG"
     gene            1465..2793
                     /locus_tag="PLQ11_04895"
     CDS             1465..2793
                     /locus_tag="PLQ11_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRK24274.1"
                     /translation="MRKASLFLAGVAAGALLFGAAELVRATGPATAAPSDTYRRLTMF
                     GDMFDKIRADYVEAPDEQKLIEAAINGMLGSLDPHSAFLDQKANEDMQRTTRGSFGGL
                     GIEVMMEDGLVKVMSPFDDSPAAQAGILANDLITHIDGTQVQGLSLNQAVDRMRGDIG
                     SKVTLKILRNKTQTLEVTLTRATIRPPVAKVRKEGDEVAYIRLSQFNEQSYDAIRKGV
                     EDMTKEIGADKLKGFILDLRNNPGGLLEQSIQVADAFLERGEIVSTRGRTPDATQRFS
                     ARAGDITKGKPVVVLINGGSASASEIVAGALQDHKRGTIIGTRSFGKASVQSIFQMRD
                     KVALKLTTQLYYTPSGKSIQGKGIDPDIQVLQDVPDDIKGKDEVKGEAALKGHIKTNE
                     DEKSGSSSYVPPDPKNDKQLIYAVDWLKSGGKLEPPKKAEAPAKDETKKN"
     gene            2887..4068
                     /locus_tag="PLQ11_04900"
     CDS             2887..4068
                     /locus_tag="PLQ11_04900"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HRK24275.1"
                     /translation="MVSDELSKPLGLDRTAASAVPGFPVARMVLGLAGVVAVAGVLWL
                     ILRTDPMGGEPYARVRIERIAQAPAVSPADDKKPNAPAGQKQRETAAEVEAASGVKVT
                     RGRDGAAPGALIIHVPDSPGSGERLAPVDRRLTERGRHGLLPKASPETGKPREVYARP
                     LNAAAVAGRPAVAVMVTGLGIGATATAEAIARLPGEVSLAFAPYGNDLERQVGGARAD
                     GHEVFLQVPMEPFDYPDNDPGPQTLLADGAEAQTLDRLHWLMGRLQGYVGLTNFMGAK
                     FTASRQALKPVLEETAKRGLMFVDDGSSARSQLVTAAAAAGLPALRGDVLLDGLDKAA
                     DVDAALARAEAAARANGVALVVGPTLPMTVDRLAGWLRTLDAKGLALVPVSSAQRFRR
                     P"
     gene            4074..4610
                     /locus_tag="PLQ11_04905"
     CDS             4074..4610
                     /locus_tag="PLQ11_04905"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008121984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HRK24276.1"
                     /translation="MTVEKKPVRALMPFRPCVGAALFNRRGEVFIGRRKSGQGPEYEA
                     LRQEWQMPQGGIDGNEPAFNAAKRELYEETNVRSAELLGVAPYWFHYDLPDSVLGKAL
                     HGKYRGQTQLWFAFRFTGEDHEIDVAHPAGGGHPAEFVEWRWERLERLPDLIVPFKRP
                     VYAQVVAAFRHHADALAG"
     gene            4658..5113
                     /gene="hisE"
                     /locus_tag="PLQ11_04910"
     CDS             4658..5113
                     /gene="hisE"
                     /locus_tag="PLQ11_04910"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HRK24277.1"
                     /translation="MPDSIQSLYMAILKERRRSPMASRTAKLLSEGTVKIAKKLAEEA
                     VEVGHEAMLKRKAAVIAESADVLYNLAVLWVDAGTRPADVWAEMKRREAMLGIAEKLP
                     KEGQPVRPRKREKADKSAKEARPVAPQRWALRRSVHWLRLGHNRTNSRI"
     gene            5117..6061
                     /locus_tag="PLQ11_04915"
     CDS             5117..6061
                     /locus_tag="PLQ11_04915"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019965234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HRK24278.1"
                     /translation="MTAPNTLGWRLTLPVIAAPMFLVSGPELVIGAMRAGIVGTFPAL
                     NQRSTEGYVEWVREIRAATADLPPEQAIFGVNLIVHPSNTRLEADVRATMDLKVPLVI
                     TSLGAVKLVVDAVHSYGGKVFHDITNRRHAEKAIEAGVDGLILVTAGAGGHAGTANPF
                     ALVAEIRRIYDGPIALAGALSNGAQVAAALAAGADFAYMGTRFIATKESRAMPGYREM
                     IHASQIRDIVYTPAISGIPANFMAGSLVKAGLDPKNLPAVDPKTHKIDMGQEARAWRD
                     VWSAGHGVVDTKDNPTVAELIGRMRREFEAARQRLSGV"
     gene            6067..6243
                     /locus_tag="PLQ11_04920"
                     /pseudo
     CDS             6067..6243
                     /locus_tag="PLQ11_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018260996.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
     gene            6324..6926
                     /locus_tag="PLQ11_04925"
     CDS             6324..6926
                     /locus_tag="PLQ11_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004679248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRK24279.1"
                     /translation="MPVPHAPELVLHLADEAIELWQKTEEELEALGLPPPFWAFAWAG
                     GQALARYVLDHPEIVRGRRVLDFASGSGLCAIAAARAGAASVLAADIDPFALSAITLN
                     SDANGVVLAATGEDVIGQAGGWDVILAGDIFYEQDLAARVFDWLSACMRDGAQVLIGD
                     PGRSYLPKEQLIELACYQVPVTRALEDAEIKKSCVWSLRS"
     gene            6935..7507
                     /locus_tag="PLQ11_04930"
     CDS             6935..7507
                     /locus_tag="PLQ11_04930"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HRK24280.1"
                     /translation="MLLRALASGDELGYSQRMARLNADDKPSFLKAHACCDAHHPSAE
                     ARLAEAEAVCARKGSRLTAQRRMVLEHLVGAGRALGAYDLISRIEATAGRKLAPITIY
                     RALDFLVEAGLVHRIESHNAFFACPAGHGPHPNAVFMICETCGSVNEAASARIAEELS
                     RLAMRSGFVPQSHIIELTGHCAACSPAQNP"
     gene            7511..8764
                     /gene="ispG"
                     /locus_tag="PLQ11_04935"
                     /gene_synonym="gcpE"
     CDS             7511..8764
                     /gene="ispG"
                     /locus_tag="PLQ11_04935"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HRK24281.1"
                     /translation="MNDSIPPSLSAAPQARHRTVAVGVGKVLVGGGAPIIVQSMTNTD
                     TADIGATVAQVAELARAGSELVRITVDRDDAAAAVPHIREQLLKRGIAVPLIGDFHYI
                     GHKLLADHPACAEALDKYRINPGNVGFKDKRDRQFSQIVEMAIKHAKPVRIGANWGSL
                     DQELLTHLMDANSRAAAPLDARAVTREAMVQSALLSAARAEEIGLGRDRIILSAKVSA
                     VQDLIAVYRMLAARSDYALHLGLTEAGMGSKGLVASSAAIGILLQEGIGDTIRYSLTP
                     EPGGDRTAEVRAAQELLQTMGFRTFVPLVAACPGCGRTTSTVFQELARDIQTWISTSM
                     PEWKTRYPGVEGLNVAVMGCIVNGPGESKHADIGISLPGTGEAPAAPVFIDGVKARTL
                     RGPTIAAEFKALVAAYIETRYGSRL"
     gene            8832..9902
                     /locus_tag="PLQ11_04940"
     CDS             8832..9902
                     /locus_tag="PLQ11_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008128826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HRK24282.1"
                     /translation="MPSSAGLVDLQVNGFAGVDFNSGTISAEALDHALEAMLATGVTM
                     CLPTIITAYPAALEQRLRALDAAVNASKLGPLMCPGYHLEGPFLNPEPGYRGCHPPGA
                     MTPADGALIARLQAVISRPILMITVAPEIPGVMDLIPRLVDQGMIVAIGHTAASIDVV
                     AAAAAAGARMSTHLGNGVPQLTHKFANPIFAQLAEDRLSAGFIADGVHLPVSALKPMM
                     RAKGFERSILVTDAATGAASAPGRYRFAGMEIDRMADGSLRLAETGSLAGSALCLDQA
                     VRNVVAWNLAPPAQAIAMASDNPRALLEPALARTGPPLERGIVEWTEHLTVQEVRIGA
                     VERHFAARQGGHAAEPGRAKPA"
     gene            complement(9924..13187)
                     /locus_tag="PLQ11_04945"
     CDS             complement(9924..13187)
                     /locus_tag="PLQ11_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRK24283.1"
                     /translation="MNAPSPGLAGRLTRAFITSPLTPLFLIVALALGLVALVTLAREE
                     EPQISVPMVDIHVRAEGLRADDAVKLVTEPLEAIIKSINSVEHVYSQTLDHGVVVTAR
                     FMVGTASDAAILRVHEKIRANIDRIPAGIPEPLVVGRGIDDVAIAVLTLTPKPEAAER
                     WTAADLTRLARELRTDIAKLDNVGLTYLVGETPEEIRITPDPERLARSGLTLQALTAK
                     IQSANSAFPAGTIRTEGRQLSLIAGRTLATIEEIANLSVTTRDDRPVYVRDVATIGLA
                     QETEVRVAHLVHDGDRFRRAPAVSIAVAKRAGANAVIIADQVLAAVERARGTLVPADI
                     EVIATRNYGETANEKANELLFHLGLATLSIVALVFFAIGRREALVVAVVIPVTILLTL
                     FSAKVMGYTLNRVSLFALIFSIGILVDDAIVVIENIARHWDMADGRDPREAAIDAVAE
                     VGNPTIVATLTVVAALLPMLFVSGLMGPYMSPIPSNASAAMIFSFFVAVTVTPWLMLK
                     LGRKPGHTHGHDQDGGLLGRLYRAAAAPVLTNKPRAWAFLLIVLVLTLGSLAMFQTQA
                     VTVKLLPFDDKPELSVMLDLPRGTSVEDTDRTLQEAAAALRPVREIATLQSHAGTAAP
                     FNFNGLVRHSYLRGEPHMGDLQLNLVPKGQRKRPSHAIALDVRERLKAVAFPPGTVVK
                     VVEPPPGPPVLATLLAEIYGPTPDIRRQVAEKVKAAFRAVPFIVDIDDTLARLPERRR
                     LTLVDEQIQFHKVEQRDVFETIRMLYGGSIVGYSHRGDSRQPIPIRVALDRSTKVLDE
                     RALAVPVPANVIPGARGVVELGDVVRIETEQPFSVIFRRNGRAAEMVMAELAGSFEAP
                     LYGMIAVQRELDRQDWSGLEKPRILLHGQPDDTARPALLWDGEWEVTWVTFRDMGAAF
                     AVAMLGIYILVVAQFGSFKLPLVILTPVPLTFIGIMLGHWLFGAPFSATSMIGFIALA
                     GIIVRNSILLVDFIRHAPRTMTVTRTTEAGTSETLIVPRPLIDILLEAGAIRFKPILL
                     TALAAMIGAVTILNDPIFQGLAISLLFGLASSTLLTLLVIPAIYIALRGGPEGAARI"
     gene            complement(13184..14146)
                     /locus_tag="PLQ11_04950"
     CDS             complement(13184..14146)
                     /locus_tag="PLQ11_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006932374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRK24284.1"
                     /translation="MMRILTLLVLLGLAGAARAGELEIKPLAITETKAVFGRVETRDV
                     VPARARIGGTLMSITVSEGDAVRQGDRIALVADQKLALQMSAADARIKALVSEQANAR
                     TEYDRARELMARGASTQQRLDQTRTALEVVGNQIAAAEAERSVIAQQAAEGAVLAPAT
                     GRVLTKPVTVGGVILPGETVATIAGGGFFLRLAIPERHAPHLKLGVDVPVDGRPGQAT
                     GTGKLAKIFPQIENGRVIADVELNTLGDFFVGERVLVHVPVATRQAIAVPQQAVMTRS
                     GIDIVRLAGGSEVAVVPGRMVETEAGPRLEILSGLRSGDRVIVP"
     gene            complement(14151..14354)
                     /locus_tag="PLQ11_04955"
     CDS             complement(14151..14354)
                     /locus_tag="PLQ11_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HRK24285.1"
                     /translation="MSIDQAVMAFAGFMVLLSLALTYFVSPHFWLLTGFVGLNMLQAA
                     FTGFCPAAMLARKLGFRAGCIFN"
BASE COUNT         2656 a         4509 c         4907 g         2502 t
ORIGIN      
        1 agttagagca tgtcttctgc gattgaccgg tatccactta atcgcgacat gctctaagaa
       61 tttcaccgtt ctggccatca gatggtaaga cgaatcagtt ccgattgctg ggatggatcg
      121 tcacttgccc gttgccggac caaaactcag ggcggatttt gtgcggcgca accggtgcgg
      181 gcgcttctgt cttgccgtga tgaccctgct gctggcggtg ggcctggccg ctgcccaggc
      241 gccgcctgcc ggcgaggcaa cgccgcaggg tgaattgcgg caggtgctcg aaaagatcca
      301 gcgcagcgaa gccgagcagt cgcggctaag gagcgagatc accgggctga agggcgatcg
      361 cgcccaactc aacaaggatc tgctcgcctc gaccgttgct gtgcgagagg cggaagtgcg
      421 tatcggtgag gcacaggccc gcctcaacga ggtgacggca agcgaacagg cgctcaaaca
      481 gtcgctggag gcacggagag gcgtcatcac cgaggtgctg gcgacgctgc aacggatggg
      541 gcggcgatcg ccgcctgcgg tactggtccg gcccgaggat atgctggaag ccatccgctc
      601 ggcgatcctg cttggtcatg tggtgcccga tatcaaggcg gaggccgagc agcttgcggc
      661 cgacctcagc gagcttgtcc ggctgcgcag ggcagcggcg gcagagcgcc agcgcctgca
      721 aggcgagcgc gaccgaatcg aagccgagag aacacggctg gcagggctga tcgatgcccg
      781 ccagagccag atcgaatcat cggaaaaggc attgggcgac gaacggggtc ggctcgacgg
      841 catggccagg caggcgcaga ctctcaaaga tttgatcgcg cggctggagc aggatgccgc
      901 cagcatgcag cgtgtccagg aacaggcgaa gaaggctcct tctcccgccc agagtcagga
      961 agatgccacc aggctggcgg cgcttgcttt cagggatccg gcgcggctgg cgccaaaaat
     1021 cgccttccag gacgctcgcg gcctgctggc gttgccgaca aatggcccgg tcatcaaggt
     1081 cttcggggcg gctgacgggt tcggcggttc ggagcgcggc attaccatcg cagcccagcc
     1141 cgggcatgtg gtgaccgcgc cgtcggatgg ctggatcctg tttgccggac cctaccgctc
     1201 ttacggtcgg atcttgatta tcaatgccgg caacggctac aatttcgtgc tgaccggatt
     1261 gcagtcgaca tcggtcgagc cgggccagtt cgtgctggcc ggggaaccgg tcggcatgat
     1321 gggatcgccc gccctcgagg cggcgcggac cggggatggt cagccggcca cctcgcacct
     1381 atatgtggaa ctgcgtaagg ataaccagcc catcgacccg acaccctggt gggcgaaggc
     1441 gaccaacgaa aaggcgcgag gatgatgcgc aaggcttctt tgtttctggc aggcgtcgca
     1501 gcgggcgccc tgttgttcgg cgcggcggag ctggtgaggg cgaccggtcc ggccacggcc
     1561 gcaccgtccg acacctaccg tcggctgacg atgttcggcg acatgttcga caaaatccgt
     1621 gccgattacg tcgaggcgcc ggacgagcag aagctgatcg aggcggcaat caacggcatg
     1681 ctcggttcgc tcgatccgca ttcggcattc cttgaccaga aggccaatga ggacatgcag
     1741 cgcacgacgc gcggttcgtt cggcggactc ggcatcgagg tgatgatgga ggatggcctg
     1801 gtcaaggtca tgagtccctt cgacgattcg cccgccgccc aggcggggat cctcgccaat
     1861 gacctgatca cccatatcga tggcacccag gttcagggcc tgtcgctcaa tcaggcggtt
     1921 gaccggatgc gtggcgatat cggcagcaag gtcaccctga agatcctgcg caacaagacc
     1981 cagacgcttg aggtgacgct gacccgcgcc accatccgtc cgccggtcgc caaggtgcgc
     2041 aaggagggcg atgaggtcgc ctacatccgg ctgtcacagt tcaacgaaca atcctatgac
     2101 gcgatccgca agggcgtcga ggacatgacg aaagagatcg gcgccgacaa gctcaagggc
     2161 ttcatcctcg atctgcgcaa caatccgggc gggctgctgg aacagtcgat ccaggtcgcc
     2221 gatgcattcc ttgagcgtgg cgagatcgtc tcgacgcgtg ggcgcacgcc ggacgcaacc
     2281 cagaggttct cggccagggc aggcgatatc accaagggca agccggtcgt ggttctgatc
     2341 aacggcgggt cggcctcggc ctcggaaatc gtcgccggtg cgctgcagga tcacaagcgc
     2401 ggcaccatca tcggaacacg ctcgttcggc aaggcatcgg tgcagtcgat cttccagatg
     2461 cgcgacaagg tggcgctgaa actgacgacg cagctctatt acacgccgtc gggcaaatcg
     2521 atccagggca aggggatcga tccggacatc caggtgctcc aggatgtgcc cgatgacatc
     2581 aagggcaagg acgaggtcaa gggcgaggcg gccctgaaag gtcatatcaa gaccaatgag
     2641 gacgaaaagt ctggatcctc gtcctatgtg ccgccggatc ccaagaacga caagcagctc
     2701 atctatgcgg tcgactggct gaagtcgggc ggcaagctcg agccgccgaa gaaggccgag
     2761 gcgccggcca aggacgagac caagaagaac tgaggccggc tgccgccgca acggcggctt
     2821 tgcctccacg gcgcagacaa gtaatctcgc agggcaggtg attcgcctgc cggttgtctt
     2881 gtttgcatgg tcagcgacga actcagcaag cccctcggtc tcgaccggac ggccgcaagc
     2941 gccgtgccgg ggtttccggt tgcgcggatg gttctggggc tggccggtgt ggtggccgtg
     3001 gccggggtgc tgtggctgat cctgcgcacc gaccctatgg gcggggagcc ctatgcccgg
     3061 gtcaggatcg agcgcatcgc ccaggcgccg gctgtctctc cggctgacga caagaagccg
     3121 aatgctccgg ccgggcagaa acagcgggaa accgccgccg aggtcgaggc tgcctccggc
     3181 gtcaaggtga cccgcgggcg cgacggtgcg gcgcccgggg cgctgatcat ccatgtgccg
     3241 gacagtcccg gcagcggcga gcggctcgcg ccggtggatc gccgcctcac cgagcgaggc
     3301 cgccatggcc tattgcccaa ggcgtcgccc gagaccggca agccgcgcga ggtctatgcc
     3361 cggccgctga atgccgctgc ggtggccggt cgtccggccg tggcggtgat ggtgaccgga
     3421 cttggtatcg gcgccaccgc aacggccgag gcgatcgcca gattgccggg tgaggtaagc
     3481 ctggcctttg cgccctacgg caacgatctt gaacgccagg ttggcggggc acgcgccgat
     3541 ggtcacgagg tgttcctcca ggtgccgatg gagccgttcg actatcctga caatgaccct
     3601 ggcccgcaga cgcttctggc ggacggggcc gaggcacaga cgctcgaccg gctgcactgg
     3661 ctgatgggac gattgcaggg gtatgtcgga ctgacgaatt tcatgggggc gaagttcacg
     3721 gcttcgcgtc aggcgctcaa accggtcctg gaagagacgg ccaaacgcgg tttgatgttt
     3781 gtcgatgacg gttcttcggc gcgaagtcag ctggtgactg ccgctgcagc ggccgggttg
     3841 ccggcactgc gtggtgacgt gctgctcgac gggctcgaca aggccgccga tgtcgatgcg
     3901 gcactggccc gggccgaagc ggccgcccgg gccaacggtg ttgcactggt tgtcggcccg
     3961 acgctgccaa tgacggttga ccgcctggcc ggctggctgc gcacgctgga cgcaaagggg
     4021 ttggcgctgg tgccggtttc gtcggcacag cggttcagga ggccgtgaat tccatgacgg
     4081 tggagaaaaa gccggtccgt gcattgatgc ctttccgccc ctgtgtcggc gccgccctgt
     4141 tcaaccgccg cggcgaggtg ttcatcggtc gccgcaagtc cggccagggc ccggaatacg
     4201 aggcattgcg gcaggagtgg cagatgccgc agggcgggat cgacggaaac gagccggcct
     4261 tcaatgcggc aaaacgcgaa ctttatgagg aaaccaacgt ccgcagtgcc gaattgctgg
     4321 gcgtagctcc ctactggttc cactatgacc tgcccgattc cgtcctcggc aaggcattgc
     4381 acggcaaata tcgcggccag acgcagttgt ggttcgcctt ccgctttact ggagaggacc
     4441 atgaaatcga tgtcgcccat ccggccgggg gaggacatcc ggccgagttt gtcgaatggc
     4501 gatgggaacg gctggagcgg ctgccggatc tgatcgtgcc gttcaagcgc ccggtttatg
     4561 cccaggtggt cgccgccttt cgtcaccatg ccgatgcgct ggcgggttga accggaaagc
     4621 cttttgcaaa cggcgcattc cgcaggatag tcggaccatg cccgattcga ttcaatccct
     4681 ttacatggcc attctcaagg agcggcgtcg ctcgcccatg gcttcgcgca cagccaagct
     4741 gctcagcgag ggcacggtca agatcgccaa gaagcttgcc gaggaggcgg tcgaggtcgg
     4801 tcatgaggcg atgctgaaac gcaaggctgc tgtgatcgcc gaaagcgccg atgtgcttta
     4861 caatcttgcc gtgctctggg tcgatgcggg gacaaggcct gccgatgtct gggcagagat
     4921 gaagcgccgc gaggccatgc tcgggattgc cgagaaactg ccgaaggaag gtcagccggt
     4981 gcgaccgcgc aagcgcgaaa aggccgacaa gagcgccaag gaggcaagac cggtcgctcc
     5041 ccagcgctgg gccttgcgcc gttcggtgca ttggttacga ttgggtcaca accgaaccaa
     5101 ttcgaggatc tgacccgtga cggctcccaa tactctcggc tggaggctga cgcttccggt
     5161 catcgcagcg ccgatgtttc tggtgtccgg cccggaactc gtgatcggcg ccatgcgtgc
     5221 cggtattgtc ggcactttcc cggcactcaa ccagcgctcg accgaggggt atgtcgaatg
     5281 ggtcagggag atccgtgccg cgactgcgga tctgccgccg gaacaggcta ttttcggggt
     5341 caacctgatc gtccatccca gcaacacacg gctggaagcg gatgtgcgcg ccacgatgga
     5401 tctcaaggtg ccgctggtca tcacctcgct gggggctgtg aagctggtgg ttgatgcggt
     5461 gcacagctac ggcggcaagg tgttccacga catcaccaac cgccggcatg cggaaaaggc
     5521 gatcgaggcg ggtgttgacg ggctgatcct ggtgacagcc ggggctggcg ggcatgccgg
     5581 cacggccaat ccgttcgcgc tggtcgcgga gatccgccgc atctacgatg gaccgatcgc
     5641 gctggccggt gcgctgtcga atggcgccca ggtggccgct gcgctcgcgg cgggcgcaga
     5701 ttttgcctat atgggcaccc gcttcatcgc gaccaaggaa agccgcgcga tgccggggta
     5761 tcgcgagatg atccacgcat cgcagatccg cgatatcgtc tacacgccgg caatttccgg
     5821 cattcctgcc aatttcatgg ctggttccct ggtcaaggcg gggctcgacc ccaagaacct
     5881 tccggccgtc gatcccaaga cccacaagat cgacatgggc caggaggcgc gcgcctggcg
     5941 cgatgtctgg tctgccggac atggcgttgt cgacaccaaa gacaatccga ccgttgcgga
     6001 actgatcggg cgcatgcgcc gggagttcga ggcggcgcgc cagcggcttt ccggcgtgtg
     6061 atggtgatgg gtccgggcaa cggacgaggc aagatcgcct ttgtcgtgac cgaggactgg
     6121 ttcttctgct cgcatttcct gccgatggcg cgggcagcgc gcgatgcggg attcgacagt
     6181 gtggtcattg cccgcgagcg cgaccatcgg caccagatcg aggcggaagg gttccggctg
     6241 gtggcacgcg accggtcgat cgtgtcatga cccgcatcgc gcccggggag gccgcaattt
     6301 tcattcagcg cgagacgcgg ctgatgccag tgcctcatgc gccggagctt gtgctgcatc
     6361 ttgccgatga ggcgatcgag ctttggcaaa agaccgagga ggaactggaa gcgctcggcc
     6421 tgccgccgcc attctgggct tttgcctggg caggcggcca ggcgctggcc cgttatgtgc
     6481 ttgatcatcc cgaaatcgtg cgcggccggc gcgtgctcga cttcgcctct gggtcgggat
     6541 tatgcgccat agcggcggca cgggcgggag ctgccagcgt gctggcagcc gatatcgatc
     6601 cgtttgccct gtcggcgatc acgctcaaca gcgatgccaa cggtgttgtc ctggccgcca
     6661 ccggcgagga cgtgatcggc caggccgggg gatgggacgt gatcctcgcc ggcgacatct
     6721 tctatgagca ggatctggcg gcccgtgtgt tcgactggct gagcgcctgc atgcgcgacg
     6781 gggcgcaggt gctgatcggt gatcctggcc gcagctatct gccgaaggag cagttgatcg
     6841 agcttgcctg ctatcaggtt ccggtgaccc gggcactgga ggatgccgag atcaagaaaa
     6901 gctgcgtctg gtcgctgcga tcatgagccc gcaggtgctg ctgagagccc tcgcatccgg
     6961 cgacgagttg ggctatagcc agcgcatggc ccgattgaac gcggatgaca agccttcctt
     7021 cctcaaggcc catgcctgct gtgacgcgca tcacccgagc gcggaggcgc gcctcgccga
     7081 ggccgaggcc gtttgcgcgc gcaagggatc gcggctgacc gcgcaacggc gcatggtgct
     7141 tgaacatctg gtcggcgccg ggcgggcgct gggagcctac gacctgattt caaggatcga
     7201 ggcgacagca gggcgcaagc tcgcgccgat cacgatctat cgcgcgctcg atttccttgt
     7261 tgaagccgga cttgttcaca ggatcgaaag tcacaatgcc tttttcgcct gcccggctgg
     7321 acacgggccg catcccaatg cggtgttcat gatctgcgag acctgcggca gcgtgaacga
     7381 ggcagcaagc gcccggatcg ccgaggaact gtccaggctt gccatgcgga gcggattcgt
     7441 tccgcaaagc cacattatcg aactgactgg ccattgtgcg gcctgctccc ctgcccagaa
     7501 cccgtagtcg atgaacgatt ctatacctcc ctccctgagc gccgcaccgc aagcacggca
     7561 ccggactgtt gccgttggcg tgggcaaagt gctggtcggt ggcggggcgc cgatcattgt
     7621 ccagtcgatg accaataccg atacggccga tatcggcgcg acggtggcgc aggtcgcgga
     7681 actggcgcgg gccggatcgg aactggtgcg catcacggtg gatcgcgacg atgcggcggc
     7741 agccgtgccg catatcaggg agcagttgct caagcgcggc attgctgtgc cattgatcgg
     7801 agatttccat tacatcggcc acaagcttct ggccgatcac cccgcctgtg ccgaagcgct
     7861 cgacaaatac cgcatcaatc cgggcaatgt cggcttcaag gacaagcgcg accggcaatt
     7921 cagccagatc gtcgaaatgg cgatcaagca tgcaaagccg gtgcggatcg gcgccaattg
     7981 gggctcgctt gatcaggagc tgctgaccca tctgatggat gccaattccc gcgccgctgc
     8041 tcccctcgat gcgcgggcgg tgacgcgcga ggccatggtg cagtcggcct tgctgtcggc
     8101 agcacgcgcc gaggagatcg gattgggcag ggaccgcatc atcctgtcgg ccaaggtctc
     8161 ggcggtgcag gatctgatcg ccgtctaccg catgctggcg gcgcgctcgg attatgcgct
     8221 acatctgggg ttgaccgagg cagggatggg ctcgaaaggc ctggttgcct cgtcggctgc
     8281 gatcggcatc ctgttgcagg aagggattgg cgacacgatc cgctattcgc tgacgccgga
     8341 accgggcggg gatcggacgg cggaagtccg cgccgcgcag gaattgctgc agaccatggg
     8401 gttccgcacc ttcgtgccgc tggtcgctgc ctgtccgggc tgcgggcgga ccacctcgac
     8461 cgtgtttcag gagctggcgc gcgatatcca gacgtggatt tccacctcaa tgccggagtg
     8521 gaagacgcgg tatcccggcg tcgagggcct caatgtcgcg gtgatgggct gcatcgtcaa
     8581 cgggccgggc gaatccaagc atgcggatat cggcatttcg ctgccgggga caggtgaagc
     8641 accggccgcg ccggtcttca tcgacggagt gaaggcaaga accctgcggg gccctaccat
     8701 cgccgccgag ttcaaggccc ttgtggccgc ctatatcgag acccgctacg gctccaggct
     8761 ctgacccagt ggcacgcagg ctgctgcggc catcaggcca acaccctgcg caaagcaaga
     8821 gggaatgtgt catgccatcg tcggccggcc tcgtcgatct tcaggtcaac ggctttgccg
     8881 gtgtcgattt caattccgga accatcagcg cggaggcgct ggatcatgcg ctggaggcga
     8941 tgctcgccac aggcgtcacc atgtgtctgc cgacgatcat cacagcctat cccgccgcac
     9001 tggagcaacg gctgagggcg ctcgacgcag cagtgaacgc gagcaagctt gggccgctga
     9061 tgtgccccgg ctatcatctt gagggcccgt tcctcaatcc tgagcccgga tatcgcggct
     9121 gccacccgcc cggagcgatg acgccggccg atggagcgct tatcgccagg cttcaggcgg
     9181 tcatttcccg gccaatcctg atgatcacgg tcgcgcctga aatccccggc gtgatggacc
     9241 tgattccacg cctcgtcgac cagggcatga tcgtcgctat cggccacacg gcggccagca
     9301 tcgatgtggt ggccgccgct gcggcggcgg gtgcgcggat gtcgacccat ctgggcaatg
     9361 gtgtgccgca gctcacgcac aaattcgcca acccgatatt tgcccagctt gcggaggatc
     9421 gcctttctgc cggcttcatc gccgatggtg tccatctgcc ggtctcggcg ctcaagccga
     9481 tgatgcgggc caaggggttt gagcgcagca tcctggtgac agatgccgca accggtgcgg
     9541 catctgcgcc cgggcgctat cgcttcgccg ggatggagat cgaccgcatg gcggacggat
     9601 cgcttcggct tgccgaaacc ggatcgttgg ccgggtccgc tttgtgcctt gaccaggctg
     9661 tccgcaatgt cgtggcctgg aaccttgcgc cgccggcgca ggccatcgcg atggcgtccg
     9721 acaatccgcg tgcgctgctt gagcctgcac tggccaggac cggacctccg ctggagcgtg
     9781 gcatcgtgga gtggaccgag catctgacgg tgcaggaggt gcgcatcggc gcggtcgaac
     9841 gtcattttgc tgcgaggcaa gggggccacg ccgcagagcc gggtcgcgca aaacccgcgt
     9901 gaccgccgtc ctgctcgtcg gcgtcagatg cgcgccgcgc cctctggtcc gccgcgcagc
     9961 gcaatgtaga tcgccggaat caccagcagc gtcagcagcg tcgacgaggc aaggccgaac
    10021 agcagcgaaa tcgccagccc ctgaaagatc ggatcgttca ggatcgtcac ggcgccgatc
    10081 atcgccgcca gcgctgtcag caggatcggc ttgaaacgga tggcgccggc ttccagcagg
    10141 atgtcgatga gcgggcgggg cacgatcagg gtttcgcttg tccccgcctc ggtggttctc
    10201 gtcactgtca tggttcgtgg cgcatggcgg atgaaatcga ccagcaggat cgagttgcgc
    10261 acgatgatcc cggccaatgc gataaagccg atcatcgacg ttgccgagaa cggggcgccg
    10321 aacagccaat gaccgagcat gatgccgatg aaggtcagcg gcaccggcgt caggatcacc
    10381 agcggcagtt tgaacgaacc gaactgcgca acgacaagaa tgtagatgcc cagcatggcc
    10441 acggcgaagg ccgcgcccat gtcacggaac gtcacccagg tgacttccca ttcgccgtcc
    10501 cacagcaggg caggacgggc ggtgtcgtcc ggctggccgt gcagcaggat ccgcggcttc
    10561 tcaaggccgc tccaatcctg acgatcgagt tcgcgctgaa cggcgatcat gccgtagagg
    10621 ggcgcctcga aactgccggc cagctccgcc atcaccatct cggcggcgcg gccattgcgg
    10681 cggaagatca ccgagaaggg ttgttcggtc tcgatccgca ccacatcgcc gagttcgacg
    10741 acgccgcgcg cgccggggat gacattggcc ggcaccggaa ccgcgagggc gcgttcgtcc
    10801 agcacctttg tggatctgtc gagggcgacg cggatcggga tcggctgccg gctgtcgcca
    10861 cgatgcgaat agccgacaat ggagccgcca tacagcatgc ggatggtctc gaacacgtcg
    10921 cgctgctcga ccttgtggaa ctggatctgt tcatcaacga gggtcaggcg ccggcgctcg
    10981 ggcaggcggg cgagcgtatc gtcgatgtcg acgatgaagg gcacggcgcg gaaggcggcc
    11041 ttgaccttct cggcaacctg gcggcggatg tccggggtcg ggccatagat ttccgcaagc
    11101 agcgtcgcca gcaccggagg ccccggcggg ggctcgacca ccttcaccac ggtgccgggc
    11161 ggaaaggcca ccgccttcag ccgctcgcgc acgtccagcg cgatggcatg gctggggcgc
    11221 ttgcgctggc ccttggggac gaggttgagt tgcaggtctc ccatatgcgg ctcgccgcgc
    11281 agataggaat ggcgtaccag cccattgaaa ttgaagggtg ctgccgtgcc ggcatggctc
    11341 tgcagggtgg cgatctcgcg caccggccgc agcgccgccg ctgcttcctg cagggtgcgg
    11401 tcggtgtctt ccaccgaggt gccgcgcggc aggtccagca tgacgctgag ttcaggcttg
    11461 tcatcgaacg gcaggagctt gaccgtgaca gcctgggtct ggaacatggc cagcgagccg
    11521 agagtgagca ccagcacgat cagcaggaag gcccaggctc gcggcttgtt ggtcagcacc
    11581 ggggcggcgg cggcgcgata gagccggccg agcaggccac catcttggtc atgaccatgc
    11641 gtgtggcccg gtttcctgcc gagtttgagc atcagccagg gcgtgaccgt caccgcgaca
    11701 aagaacgaga agatcatcgc ggctgaggcg ttcgacggga tcgggctcat gtagggtccc
    11761 atcagcccgg agacgaacag catcggcagc agcgccgcga cgacggtcag ggtggcaacg
    11821 atggtcggat tgccgacctc ggccaccgca tcgatggccg cctcgcgcgg atcacggccg
    11881 tccgccatgt cccaatggcg cgcgatgttc tcgatgacca cgatcgcgtc atcgaccaga
    11941 atgccgatgg aaaagatcag cgcaaacagg ctgacacgat tgagcgtgta gcccatcacc
    12001 ttggccgaaa acagcgtcag caggatggtc accggtatga caacggcgac caccagagct
    12061 tcgcggcggc cgatggcgaa aaacaccagt gcaacgatcg aaagggttgc cagtccgagg
    12121 tggaacagca gttcgttggc cttctcgttg gccgtttctc cgtagttgcg tgttgcgatc
    12181 acttcgatat cggccggaac cagcgtgcct ctggcgcgct cgactgccgc cagaacctgg
    12241 tcggcgatga tgactgcatt ggcgcccgcc cgcttggcca ccgcaatcga cacggcgggt
    12301 gcgcggcgga agcggtcgcc atcatggacg agatgggcca cgcgcacctc cgtctcctgg
    12361 gcgaggccga ttgtggctac atcgcggacg tatacgggcc gatcatcgcg tgttgtcacg
    12421 gagagattgg cgatctcctc gatggtcgcg agcgttcggc cggcgatgag tgaaagctgg
    12481 cggccttcgg tacggatggt gccggccggg aaggcgctat tggcgctctg gatcttggcc
    12541 gtcagggcct gcaacgtaag cccggaccgt gccagccgtt cgggatccgg cgtgatgcgg
    12601 atttcttccg gggtttcgcc gacgagatag gtcaggccga cattgtcgag cttggcaatg
    12661 tcggtgcgca attcccgggc aagccgggtc agatcggcgg cggtccagcg ctccgcagct
    12721 tccggcttgg gcgtcagtgt cagcacggcg atcgccacat catcgatgcc gcgccccacg
    12781 accaggggct ccgggatgcc ggccgggatg cggtcgatgt tggcgcggat cttttcatgc
    12841 acgcgcagga tcgccgcgtc cgacgccgtg ccaaccatga agcgcgccgt gacgacaaca
    12901 ccgtggtcca gcgtctgtga atagacgtgt tcaaccgagt tgatcgactt gatgatggct
    12961 tccagcggct ctgtgacgag cttgaccgca tcgtccgccc gcaagccttc ggcgcgcacg
    13021 tgaatgtcga ccatcggcac ggagatctgc ggctcttcct cgcgcgccag cgtcaccagc
    13081 gcaaccagtc ctagggcgag ggcgacgatc agaaacagcg gtgtcagcgg agaggtgatg
    13141 aaagcgcggg tcagccgccc cgccaggcca ggagagggag cattcacggc acgatcaccc
    13201 ggtcgcccga cctcaggccg gaaaggattt caagtctcgg acccgcctcg gtttcgacca
    13261 ttctgcccgg caccaccgca acctcgctgc cgccggcaag ccgcacgata tcgatgcccg
    13321 aacgggtcat gacggcctgt tgcggcacgg caatggcctg ccgggtggcc acagggacat
    13381 gcaccagcac ccgttcaccg acaaagaaat cgcccaatgt gttgagttca acgtcggcga
    13441 tgacccggcc gttctcgatc tgcggaaaga tttttgccag cttgccggtg ccggtcgcct
    13501 ggccggggcg gccatcaacc ggcacgtcca cacccagctt gagatggggc gcatggcgct
    13561 ccggtattgc caagcgcagg aagaagccgc caccggcgat ggtagcgacg gtctcgcccg
    13621 gcagaatgac accgcccacc gtcaccggct tggtcagcac gcgacccgtt gccggggcca
    13681 gaaccgcgcc ttccgccgcc tgctgggcaa tgacggagcg ctccgcttca gccgccgcga
    13741 tctgattgcc caccacttcg agggcggtgc gggtctgatc gaggcgctgc tgggtgctgg
    13801 cgccgcgcgc catcagttca cgggcgcggt catattctgt ccgggcattg gcctgttcgg
    13861 aaaccagggc cttgatgcgc gcatccgcag cgctcatctg cagcgccagc ttctgatcgg
    13921 cgaccagcgc aatgcgatca ccctgcctga ccgcatctcc ctcgctcacg gtgatcgaca
    13981 tcagcgtgcc gccgatgcgg gcgcgggcgg gaacgacatc ccgcgtttcc acgcgcccga
    14041 acacggcctt cgtctcggtg atcgccaacg gcttgatctc aagctcgccg gctctggctg
    14101 caccggccag tccgagcaaa acaagcagcg tcaaaatgcg catcatggcc tcagttgaag
    14161 atgcagccgg cgcgaaaccc cagcttcctg gccagcatgg cagcagggca gaagccggta
    14221 aaggcagcct gaagcatgtt caacccgaca aagccggtca gaagccagaa atggggcgat
    14281 acgaaatagg tcaatgccag cgataacagg accataaagc cggcaaaggc cataactgct
    14341 tgatcaatcg acacgggagc ctccataggt gttgcggccc ctaatatatt agaaatctct
    14401 aatattgcaa ggcctgacga actcccatct ttctgatcgt gcgacagttg agaggccgtt
    14461 gccagtgtct cctggatgaa tcagagccag gggccgctgg ccacagtgcc gacgggccca
    14521 agaagagcgc tgaccggagg ccaacacccg gccgggtcgg acaatagtgc tcta
//
LOCUS       DAOMXG010000048        14481 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_2301_length_14481_cov_10.730001, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000048 DAOMXG010000000
VERSION     DAOMXG010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 14481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14481
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2301_length_14481_cov_10.730001"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..900)
                     /locus_tag="PLQ11_04960"
     CDS             complement(52..900)
                     /locus_tag="PLQ11_04960"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRK24286.1"
                     /translation="MTGPLRDRDWFEQQYNPRLTVTDVQGIFDRWTAVSAATRKGRPC
                     RLDLPYGPHPREVIDLFPADRPRGALVFLHGGYWRALSTKEHSFVAPAWLEAGFSVFL
                     VNYPLCPQVTIKDIVASCRRAMATLWPMLGEAERRHVLVAGHSAGGYLAAAMAATDWA
                     APGLPAVALSGALSLSGVFDLPPLLNTSMNELIRLTPETARDWSLTRAAPATTVPLSL
                     ALGADEPGEFHRQSFALAEAWHMPAGSVQALAGHNHFTIVEDFADPAGRSFALAMQRV
                     GLAPTD"
     gene            complement(916..1827)
                     /locus_tag="PLQ11_04965"
     CDS             complement(916..1827)
                     /locus_tag="PLQ11_04965"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017275929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRK24287.1"
                     /translation="MSKERIGFIGVGLMGHGMAKNLVTKGFPLTIMGNRNRAPVDNLV
                     SLGAKEVKTARDVAASSDIVFLCVTDSPTVEALVSGPQGLKAGAHRGLVIVDCSTADP
                     TSTRRIIDDLAPFGVTFCDAPLGGTPVQAEEGKLFAMVGCDEATFTRIRPAIEAWAGR
                     VQHFGPATTGHKLKLINNFIAQGYGAIYAEAFALARKSGIAPETLFNVINGGRMDCGY
                     FQTFAAYAARGELESHKFSIRNALKDLRYVETMANAAVVSNPVGNAVKNTYALATAQG
                     EGDTFVPLVVDVIATQNGLPPYHAKKR"
     gene            complement(1927..2814)
                     /locus_tag="PLQ11_04970"
     CDS             complement(1927..2814)
                     /locus_tag="PLQ11_04970"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRK24288.1"
                     /translation="MATIAFIGLGAMGLPMADNLIKRQHKVRGFDFRKEALEVHQRNG
                     GIACASAAEAATGAEALVLMVVNGAQAKSALFDAAALDALNPGAVVMLMATCSAADAR
                     EIGEKVKASGRKFVDCPVSGGVVGARAGLLTIMAAAPGEDFAAAEPILRVFGDKVFLV
                     GKHWGQGSTVKTLNQLLCGVHIVAAAEAMALAEKAGLDTAMMLKILGNSAAGSWMLNN
                     RGPRMLEAEPEVTSAVDIFVKDLSLVLDAGRAEKVALPLSAAAHQMFLAASGAGHGAA
                     DDSQVIRTYRAMNGTEPKS"
     gene            complement(2869..3741)
                     /locus_tag="PLQ11_04975"
     CDS             complement(2869..3741)
                     /locus_tag="PLQ11_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRK24289.1"
                     /translation="MTLEDHSDGMSYLETTRSRLITLWLPLGLIVFVLLFPFYWMALT
                     AIKPDNQLLDFNTYNPLWTWEPTFKHINKLLFESNYPRWLWNTFYVAFAATFLSLIAS
                     VFAAYAITRLRFRGSSTVGAMIFLAYLVPPSILFIPLSTVIVAYGLFDSPLALILTYP
                     TILIPFCTWLLMGYFKTIPFELEECALMDGATRWQILWKIILPLAVPGLISASIFALT
                     LCWNEFIYALTFISSVPNKTVPVAVVNEFVDGDIFRWGSLMAGALVGSLPLVILYSFF
                     VEYYVSAMTGAVKE"
     gene            complement(3816..4772)
                     /locus_tag="PLQ11_04980"
     CDS             complement(3816..4772)
                     /locus_tag="PLQ11_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRK24290.1"
                     /translation="MSATAPSAGKRQPFKEPTTWERLQSDRNWLGFWFMLPAAAILLL
                     FLAYPLAKGIWMSLTDVKIGRAGQFIGLENYIELISYDKTFLRATAFTLVYTAVASVI
                     KFALGLYLALLLNNNMPFKSFIRSIVLIPFIVPTVLSAIAFWWIFDTQFSIISWFLKK
                     TGLISQNINFLGDPLLAQATVIFCNIWRGIPFIAITLLAGLQTVSPSLYEAATLDGAT
                     RWQIFKNITYPLLTPIIAVVMTFSVLFTFTDFQLIWAMTRGGPVNATHLMATYSYQKG
                     ILGGQLGEGAAIATAMVPFLLAAIMVSWFGLQQRKWQQGAKE"
     gene            complement(4868..6190)
                     /locus_tag="PLQ11_04985"
     CDS             complement(4868..6190)
                     /locus_tag="PLQ11_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018262606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRK24291.1"
                     /translation="MTFTRRSILKGSAGLAAAAGLSSEALLGFAKSWAQAAPWKPEAG
                     ATVKLMRWKRFLQQEEDAFLKMVDAFQKATGVKVEVTNESFDDIQPKASVAANTGQGP
                     DAVWPLHSTPHLFPEKLLDVTDVADYLGKKYGGWVPVAELYGKSKGKWIALPIAINGG
                     YINYRIKAMNDAGFSKFPETTDEFLALCKALKAKNTPAGFALGHASGDANAWIHWLLW
                     SHGGFLVDKDDKVIINSPETAKALEYCKALYETFIPGTASWNDSNNNKAFLSGELFLT
                     SNGISIYAAAKNSGDPKQLAIAEDMDHAFYPLGPSGKPAELQLVFPMMIFNYTKVPNA
                     AKALAAFMLEAENFNPWLEGAQGYLTHLLNAYDNNPVWAADKKRAPYREAAKRTLPIS
                     GLGSLGEKAATAVADFIIVDMFANYCTGREDLKGAMAQAERQARRIYR"
     gene            complement(6349..7428)
                     /gene="ugpC"
                     /locus_tag="PLQ11_04990"
     CDS             complement(6349..7428)
                     /gene="ugpC"
                     /locus_tag="PLQ11_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HRK24292.1"
                     /translation="MASVTVRDVRKAFGNVQIIKGVNIDIEDGEFVILVGPSGCGKST
                     LLRMIAGLENITAGEIAIGGRVVNNVPPKERDIAMVFQNYALYPHMTVKDNMAFSLKL
                     RNAAQTEIDERVSKAAKILGLTPLLERYPRQLSGGQRQRVAMGRAIVRDPQVFLFDEP
                     LSNLDAKLRVQMRSEIKELHQRLKTTTVYVTHDQIEAMTMADKIVVMHDGIVEQIGAP
                     LDLYDRPDNLFVAGFIGSPAMNFIKGVARRGQFVADAGFTLSLPDTSANVDGQAVTLG
                     LRPEYIRLDPKGFAAEVVVIEPTGSETQVALRIGKGDAVQEIIAVFRERISARPGDVM
                     HVLPMEGRVHLFSSETQQRLSLAGA"
     gene            7623..8390
                     /locus_tag="PLQ11_04995"
     CDS             7623..8390
                     /locus_tag="PLQ11_04995"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HRK24293.1"
                     /translation="MNVPNDVLRPPLGNPDSLFSQVAEHIALLIVSGSVAVGDLIPNE
                     TTISGPLSVSRSVYREAVKFLSAKGLVESRPKSGTRAAPATAWNLLDPDVLRWTFQAG
                     ASDQFIRDLYELRIMIEPGAARMAAERRTPAQAAAIRDAYETMASTEPYSTENIRADV
                     LFHDLLLDACGNGAFRCLRSVVMTTLMWSLALQKGTPRQDYAPALGDHARVCQAIETQ
                     DGARAEAVMRLLVHDALDDTLRRFRRRADAAKRAAAE"
     gene            8669..8950
                     /locus_tag="PLQ11_05000"
     CDS             8669..8950
                     /locus_tag="PLQ11_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRK24294.1"
                     /translation="MSSTFEKVADIIAETADIDRTSITEASHAIDDLGIDSLAFLDIA
                     FAIDKAFGIKLPLEQWTQEVNEGKAPAENYFVMKNLCARIDELVAAKKA"
     gene            8988..9455
                     /locus_tag="PLQ11_05005"
     CDS             8988..9455
                     /locus_tag="PLQ11_05005"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HRK24295.1"
                     /translation="MKLEYFQMIDRVVSLDRETLRLVATSRIPSISPIFEGHFPGHPL
                     MPGVLLIETMAQTSGYLLLGLNNFTAMPFLTKVVNANLRRFLGPDTPIEMEATLLHDG
                     SGYAVTKAKITTGGKAVCDAEIMFKILPFPEDLGAKMRANAASLGMVVTEPAA"
     gene            9452..10651
                     /locus_tag="PLQ11_05010"
     CDS             9452..10651
                     /locus_tag="PLQ11_05010"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HRK24296.1"
                     /translation="MSGRDVVITGVGLVSSLGEGLEAHGAALADAGHVHVDATTYAPY
                     AVHPATALELDRQIPKKLDQKQMEPWQRLGTYAAGLALEMAGLKGGKDLLGETQLIVA
                     AGGGERDYAVDSQILSQMPGAEKPEVLLNEKLMSELRPTLFLAQLSNLLAGNISIVHG
                     VTGASRTFMGEEQCGVDAFRIAFERIRAGQGDRFLVGGSYNAERADSLLIFAMGGFLR
                     RAGYRPVDARDDDPGFILGSGGAFLMLEAREAAEARGATILARLDSVAAMRTRRKPGA
                     VQAALGKALQNASASSGGSPLVISGATGVAGLTAEELAAIGSALPGAPVRIAGNRVGH
                     VLEAHFPVSLALAVAALRQGAVYPPTPGSAVEAPYAGAPDCVIITSVGHMRGEGLAVV
                     AAEQGAA"
     gene            10648..11922
                     /locus_tag="PLQ11_05015"
     CDS             10648..11922
                     /locus_tag="PLQ11_05015"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HRK24297.1"
                     /translation="MKDRFNRPVVVVTGMGMVTSLGRGKADNWAALTAGRSGIRRISR
                     FAVEGLKTTIAGTVDFVPAEPFCAAQLSENFARLAAEEAIGEAAIGMKGDFPGPLFVA
                     VPPVEMEWPQRVDLARASGAGSEVTYTDLLRAAGTGHFKPWHDLFLFGTVADRLADDF
                     GTKGSPISLSTACSSGATAIQLGVEAIRRGETEAALCIGTDGSVQAEALIRFSLLSAL
                     STQNDPPEGASKPFSKNRDGFVMGEGAAALVLESYASAKARGARILGILSGCGEKGDG
                     FHRTRTSPDGMPAIRAMRDAIADAGLTPDDIDYVNAHGTSTPENDKMECLGCMTVMGE
                     RMRSVPISSNKSMIGHTLTAAGAVEAVISLMTLANQRIPPTINHHLPDPAIPLDVVPN
                     AARDARVRHVMSNSFGFGGQNTVLLLSGEVPA"
     gene            11919..12668
                     /locus_tag="PLQ11_05020"
     CDS             11919..12668
                     /locus_tag="PLQ11_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRK24298.1"
                     /translation="MSGRVLVTGGGKGVGAAIVRALAEAGHDVDFTYRTSSAAAEALA
                     AEIAAAHPGRTLRALPLDLADREAVERFSTEIEDTVYFGFVHNAGQSYDALAAMMAQD
                     KAEAIMQVNFWSMTRLAKAVLRPMLRARSGRIVGIGSVAALEGNQGNAAYAATKGAML
                     SYMRTLAVESAKRGVTVNYIAPGFIDTDMLAPYANYRAQMEKQIPAGRFAQPDDIAAL
                     VTFLLSPGAAYITGAVLPVDGGLTATIAFQR"
     gene            12779..13801
                     /locus_tag="PLQ11_05025"
     CDS             12779..13801
                     /locus_tag="PLQ11_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRK24299.1"
                     /translation="MRALQLFGDRDLRLTRLEPPPHPGRGEVQVRVHAVGLNHIDVWG
                     FRGMAFAKRKMPLIVGAEAAGVVASVGEDVTHVDIGDRVVMYGARTCGHCTMCVKGRD
                     NLCENVGGIMGFHIDGFACDFVNMPQRLLVKVPDGVSFAEAACAPIAFGTVQHMLFDN
                     ARLEPGETILVQAGGSGIGTAAIRMAKAIGCTVITTVGDDDKAAKAKALGADHVINYR
                     EERFEGVVRKITGRKGVDVVFEHTGVDTWQGSLLSMKKGGRLVTCGSTSGISTTMNLM
                     QLFQQQYKIFGSFGCSIRNIEQSLEKMAQGIRPVIDTEFPLADFAKGLDRLEGRKVFG
                     KIIVSF"
     gene            13801..>14481
                     /locus_tag="PLQ11_05030"
     CDS             13801..>14481
                     /locus_tag="PLQ11_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HRK24300.1"
                     /translation="MPLVWKIRAWRALNTAGGWLMLPLVRLVFGFLKLLGPDRAADLG
                     GWLTRSIGPRLKVHRVGLANLRAAYPDKTEDEIAAIALGAWDNLGRTGAEYAHLGSLF
                     DWDADNPDGPSRTEVSGIEHFEALRDDGRPGIIFSAHLANWELPAICAAKYGLQVTAV
                     FRAPNNPAVNRVVHEIRSGTMGGLEAARAGAAFAMKEALEKGGHLGMLIDQHFTRGVP
                     VTFFGRTAM"
BASE COUNT         2739 a         4525 c         4614 g         2603 t
ORIGIN      
        1 tgatctacat cgcagcagcc gtcatcaatt cacgatgaat ccccacgcgc cttagtctgt
       61 cggggccaga ccaacgcgct gcatggcaag cgcaaagctc cgcccggccg gatctgcgaa
      121 atcctcgaca attgtgaaat ggttgtggcc ggcgagagcc tgaaccgagc ctgccggcat
      181 atgccaggct tcggccagag caaaactctg acgatggaac tctcccggtt catcggcacc
      241 aagggcaagg gacagcggca ccgtcgtcgc cggtgcggca cgggtgaggc tccagtctcg
      301 tgcggtctcg ggcgtcaacc ggatcaactc gttcatgctg gtgttgagca gcggtggcaa
      361 atcgaacacg ccggatagcg acagggcccc ggacagggcg acagcgggca accccggcgc
      421 cgcccaatcg gtcgccgcca tcgcggcagc aagatagccg ccggccgaat gacccgcgac
      481 caagacatgg cggcgctcgg cctcccccag catcggccag agcgttgcca tggcgcgccg
      541 gcaggaggcg acgatatcct tgatcgttac ctgcggacac agcggataat tgaccagaaa
      601 cacgctgaac ccggcctcaa gccaggccgg ggcgacgaaa ctgtgttcct tggtcgacag
      661 cgcccgccaa tagccaccat gcaggaaaac cagcgcgccg cgcggccgat ccgccgggaa
      721 caggtcgatc acctcccgcg gatgcggacc atagggaagg tctaggcggc agggacggcc
      781 cttccgggtg gccgccgaaa ccgccgtcca gcgatcgaaa atgccctgca catccgtaac
      841 cgtcagtcgc gggttgtatt gctgctcgaa ccagtcgcga tcacggaggg ggccggtcat
      901 gacggggacc ccggctcaac gcttcttggc atgatagggc ggcaatccat tctgggtcgc
      961 gatcacatcg accacgagcg gtacgaacgt atcgccctct ccctgcgcgg tagcgagcgc
     1021 ataggtgttc ttgaccgcgt tgccgaccgg gttggacacc actgccgcat tggccatggt
     1081 ctcgacatag cgcaggtcct tcagcgcatt gcggatcgaa aacttgtggc tttcaagctc
     1141 gcccctggca gcataggccg caaaggtctg gaaatagccg caatccattc gcccgccgtt
     1201 gatgacgttg aacaaagttt ccggcgcaat cccggatttc cgggcgaggg cgaatgcctc
     1261 cgcatagatg gcgccatagc cctgcgcgat gaagttgttg atgagcttga gcttgtgtcc
     1321 ggtggttgcc gggccgaaat gctgcacgcg gccggcccag gcctcgatgg caggtctgat
     1381 gcgcgtgaag gtcgcttcat cgcagccgac catggcgaac agcttgccct cctcggcctg
     1441 caccggggtc ccacccaggg gggcatcaca gaaagtgacg ccgaacgggg caagatcgtc
     1501 gatgatccgc cgggtggagg tggggtctgc ggtggaacaa tcgacgatga caagaccccg
     1561 gtgcgcaccg gccttcagcc cctgcgggcc ggataccaga gcttccacgg tcggggaatc
     1621 ggtgacgcag agaaaaacga tgtcggaact ggcggccacg tccctggccg ttttcacctc
     1681 tttcgcgccg aggctgacga gattgtcaac tggcgcgcgg ttgcggttgc ccatgatcgt
     1741 cagcgggaac cccttggtca cgagattctt ggccatgccg tggcccatga ggccgacgcc
     1801 gatgaaaccg atgcgttcct tgctcatagt cccatgcctt tctctcggct gacaccggtc
     1861 caatcattgc actgcggcac gtcaaccacg ctttccggtt tcaactggcc cgatgcacat
     1921 gcagcctcag ctcttcggtt cggttccgtt catcgcccga taggtgcgga tcacctggct
     1981 gtcatcggcg gcgccgtgtc cggcgccgga agcggccaga aacatctggt gtgctgccgc
     2041 cgacaatggc agggccacct tctcggcccg gccggcatcg agcaccagcg acaggtcctt
     2101 gacgaagatg tcgacagcgc tggtgacctc cggttcggct tccagcatgc gcggaccgcg
     2161 attgttgagc atccaggacc ccgccgccga gttgccaagg atcttcagca tcattgcagt
     2221 atcaagcccc gccttctcgg ccaatgccat ggcctcggcc gcagcgacga tatggacccc
     2281 gcacaggagc tggttcaagg tcttgaccgt cgacccctga ccccaatgtt tgccaacgag
     2341 aaacaccttg tcgccaaata cccgcaggat aggctccgcc gccgcaaaat cctctccggg
     2401 tgccgcagcc atgatggtca gcaagccggc acgggcaccg acaacgccgc cagagaccgg
     2461 acagtcaaca aatttgcgcc ctgaagcctt gaccttttct ccgatctcgc gcgcatcggc
     2521 cgccgagcag gtcgccatca acatgacaac agcaccgggg ttgagcgcat cgagcgcggc
     2581 ggcatcgaac agggcggatt tggcctgagc accgttgacc accatcagca ccagggcctc
     2641 ggcgccggtt gccgcctctg cggccgaggc gcaggcaatg cccccgttgc gctgatgaac
     2701 ctccagcgcc tctttcctga aatcgaagcc gcgcaccttg tgctggcgct tgatcaggtt
     2761 gtcggccatg ggcagaccca tggcgccgag accgatgaag gcaatggtgg ccattccaga
     2821 tactcccatc gtcatggccg acgcggccgg tgctggcggc atgaacgcct attctttcac
     2881 cgcgccggtc atcgccgaga cgtaatattc gacgaagaag gaatagagga tcaccaaagg
     2941 cagcgagccg accagcgcac ccgccatcag cgagccccag cggaagatgt cgccatcgac
     3001 gaattcgttg accaccgcca ccggcaccgt cttgttcggc accgaggaga taaaggtcag
     3061 cgcgtagatg aactcgttcc agcacagggt gagcgcgaag atcgaagccg agatcaagcc
     3121 aggcaccgcc agcggcagaa tgatcttcca caggatctgc cagcgcgtcg ccccgtccat
     3181 cagcgcgcat tcctcaagct cgaagggaat cgtcttgaaa tagcccatca acagccaggt
     3241 gcagaacggg atgaggatcg tcggataggt caggatcagc gcgagcggcg aatcgaacag
     3301 cccgtaggca acgatgacgg tcgacagcgg gatgaacagg atcgaaggcg gcaccagata
     3361 ggcgaggaaa atcatcgcgc cgacagtgga cgagcccctg aagcgcagcc gggtgatcgc
     3421 ataggcagca aacaccgagg cgatcagcga caggaaggtc gccgcaaacg cgacataaaa
     3481 agtgttccac agccagcgcg ggtaatttga ttcaaacagc agcttgttga tgtgcttgaa
     3541 cgtcggctcc caggtccaca gcggattgta ggtgttgaaa tcgagaagct gattgtcggg
     3601 tttgatcgcc gtcagcgcca tccagtagaa cgggaacaaa agaacgaaca cgatcagccc
     3661 gagcggcagc cagagggtga tcaagcggct gcgggtcgtc tccagatagc tcatgccatc
     3721 ggaatgatcc tcaagcgtca tcacctcatg ggccgtgacc ctgtgctggc ccgcgccttg
     3781 cgtgttgggc gaagcgatgg gagaaacggg actggtcatt ctttggctcc ctgctgccac
     3841 ttgcgctgct gaaggccgaa ccacgacacc atgatcgccg ccagcaggaa cggcaccatc
     3901 gcggtggcaa tcgccgcgcc ctcgccaagc tgtccgccga gaatgccctt ctggtaggaa
     3961 taggtcgcca tcagatgggt ggcgttcacc ggcccgccgc gggtcatcgc ccagatcagc
     4021 tggaaatcgg tgaaggtgaa cagcaccgaa aaggtcatca ccacggcgat gatcggtgtc
     4081 agcagcggat atgtgatgtt cttgaagatc tgccagcggg tcgccccgtc cagcgtcgct
     4141 gcttcataaa gcgaaggcga cactgtttga aggccggcca gcagggtgat ggcgatgaac
     4201 gggatgccgc gccagatgtt gcagaagatc acggtcgcct gcgccagcag cggatcgcca
     4261 aggaaattga tgttctggct gatcaggccg gtctttttga gaaaccacga aatgatcgag
     4321 aactgcgtgt cgaaaatcca ccagaaggcg atcgccgaca gcaccgtggg cacgatgaag
     4381 gggatcagca cgatcgagcg gatgaacgac ttgaacggca tgttgttgtt gagcagcagc
     4441 gccagataga gacccagcgc gaacttgatg accgaggcga ccgccgtata gaccagcgtg
     4501 aacgcggtcg cccgcaggaa cgtcttgtcg tagctgatca gctctatgta gttctcgagc
     4561 ccgatgaact ggccggcacg accgatcttg acgtcggtca ggctcatcca gatgcccttg
     4621 gccagcggat aggcgagaaa cagcaacaga atggccgccg ccggcagcat gaaccagaag
     4681 ccgagccagt ttcggtctga ctggagacgc tcccaggtcg tgggttcctt gaaaggctgg
     4741 cgtttgccgg cagagggggc agtcgcactc atcgcttggg ttcccggaga gaggcgagcc
     4801 atcctgccgc agaaggcggg acagcggatg gcaggcggcg ggagaaaacc ccgccgccgc
     4861 ccatttctta gcggtagatg cggcgggcct gacgctcggc ctgcgccatc gcgcccttca
     4921 ggtcctcgcg gccggtgcag taattggcga acatgtcgac gatgatgaaa tcggcaaccg
     4981 cagtcgccgc cttctcgccg agcgaaccga gtccggagat cggcagcgta cgcttggccg
     5041 cctcgcgata cggggcgcgc ttcttgtccg ccgcccacac cgggttgttg tcataggcgt
     5101 tgagcagatg ggtcagatat ccctgcgcgc cttccagcca cgggttgaag ttctccgctt
     5161 ccagcatgaa ggctgccaac gccttggcgg cgttcggcac cttggtgtaa ttgaagatca
     5221 tcatcgggaa cacgagctgc agttccgccg gcttgcccga ggggccaagc ggatagaacg
     5281 catggtccat gtcctcggcg atggccagct gcttgggatc acccgagttc ttggccgcgg
     5341 catagatcga gatgccgttc gaggtgagga acaattcgcc ggagaggaac gccttgttgt
     5401 tgttggaatc gttccacgac gcggtgcctg gaatgaaggt ctcgtagagc gccttgcaat
     5461 attccagcgc cttggcggtt tccggcgagt tgatgatgac cttgtcgtcc ttgtcgacaa
     5521 ggaagccgcc atggctccac agcagccagt gaatccaggc attggcatcg cccgaggcat
     5581 ggccgagcgc aaagccggcc ggagtgttct tggccttgag cgccttgcac aatgccagga
     5641 actcgtcggt cgtttccggg aatttggaga agccggcatc attcatcgcc ttgatgcgat
     5701 agttgatgta gccgccgttg atggcgatcg gcagggcgat ccacttgccc ttggacttgc
     5761 cataaagctc ggcaaccggc acccagccac catatttctt gccgaggtaa tcggcgacat
     5821 ccgtgacgtc gagcagcttt tccgggaaaa gatgcggggt cgaatgcagc ggccacaccg
     5881 catccgggcc ctgcccggta ttggccgcca ccgaggcttt gggctggatg tcgtcgaagg
     5941 attcgttggt cacctcgacc ttgacgccgg tcgccttctg gaaggcatcg accatcttga
     6001 ggaaagcgtc ctcttcctgc tggaggaaac gcttccagcg catcagcttg acagtggcgc
     6061 cggcttccgg cttccacggc gcggcctgag cccacgactt ggcgaagccg agcaaggctt
     6121 cggaactaag gccggcggcg gcggccagac cagcgctacc cttgagaatg ctgcggcgag
     6181 taaacgtcat ggtatcctcc gtttgacgtc tggtgtgaat gcgtcgtcaa ttcacatggt
     6241 cgggcatatt gcgggcgatt gatccgtagc cagccagtcg ccctatgctc tatgctttgg
     6301 gaccccgagc gcggcgcatg ccgcgccggt acgtggcacc atgattcctc atgcccctgc
     6361 cagggacaag cgctgctgcg tctcactcga gaacaggtga acgcgccctt ccatcggcaa
     6421 gacatgcatc acgtcgcccg gccttgccga gatccgctcg cggaatacgg cgatgatttc
     6481 ctgaaccgca tcgccctttc ctatccgcag ggcgacctgg gtttccgaac cggtcggctc
     6541 gatcaccacc acctcggcgg caaagccttt cggatcgagc cggatatatt cgggtcgcag
     6601 cccgagggtc accgcctggc cgtccacatt ggcgctggtg tcgggcagcg acagcgtaaa
     6661 gcccgcatcg gcgacaaact gaccgcggcg agccacaccc ttgatgaaat tcatcgccgg
     6721 cgagccgatg aacccggcga caaacagatt gtcgggccga tcgtaaagat ccagcggcgc
     6781 cccgatctgc tcgacgatgc cgtcatgcat caccacgatc ttgtcggcca tggtcatcgc
     6841 ctcgatctga tcgtgggtga catagaccgt ggtggtcttg agccgctggt gcagctcctt
     6901 gatctccgaa cgcatctgca cgcgcagctt ggcatcgaga ttggacaagg gctcgtcgaa
     6961 caggaacacc tgcggatcgc gcacgatggc acggcccatg gcaacgcgct ggcgttggcc
     7021 gcccgaaagc tggcgcgggt agcgctcaag caggggggtg aggccaagga tcttggcggc
     7081 cttgctgaca cgctcgtcga tttccgtctg cgcggcattc cgcagcttga gcgaaaaggc
     7141 catgttgtcc ttcacagtca tatgcgggta gagcgcatag ttctggaaca ccatggcaat
     7201 gtcgcgctcc ttcggcggga cattgttgac gacgcggccg ccgatggcga tctcgcccgc
     7261 cgtgatattc tcgaggccgg cgatcatgcg caacagcgtg gatttcccgc agccggaggg
     7321 gccaaccaga atgacgaatt cgccgtcctc gatgtcgatg ttcacaccct tgatgatctg
     7381 cacattgccg aacgccttgc gcacgtcgcg gaccgtcacc gacgccatct acatcctccc
     7441 tgagccgacc ccgtccgggt gctgcccgcc tgtagggccc gatctggtgc cgggcttctt
     7501 ggccttcgcc caaagacgaa agaccttgca ctcgattagt cagattatat gatgtcattt
     7561 gtcaagcatg aatgcgccag gcgccgaaaa atgcgccgag gcgtccagcc gggagaccag
     7621 cgatgaacgt cccaaacgat gttttgcgtc caccgcttgg caatcccgac agcctgtttt
     7681 cccaggttgc cgaacatatt gctctgctga tcgtctcggg aagcgtagcg gtcggcgacc
     7741 tgattccgaa cgagacaacc atcagcggcc ccttgtcggt atcgcgcagc gtttaccgcg
     7801 aggcggtcaa gttcctttcc gccaaggggc tggttgaatc gcgccccaaa agcggaaccc
     7861 gcgccgcgcc ggcgaccgcc tggaacctgc tcgacccgga cgtcctgcga tggacgttcc
     7921 aggccggggc gagcgatcaa ttcattcgtg atctctatga gttacggatc atgatcgagc
     7981 ctggagcggc aagaatggcg gctgagcgcc gcacaccggc gcaggcggct gcaatccgcg
     8041 atgcctatga gacaatggcc agcaccgaac cctactcgac tgaaaatatc cgggccgatg
     8101 tcctgtttca cgacctgctg ctcgatgcct gcggcaatgg cgctttccgt tgcctgcgga
     8161 gcgttgtgat gaccacgctg atgtggtcac tggcactgca aaagggcacg ccccggcagg
     8221 actatgctcc cgccctgggc gatcacgccc gcgtctgtca ggccatcgaa acacaggatg
     8281 gggcacgcgc ggaagccgtg atgcggctct tggtccatga tgcgctggac gatacgctgc
     8341 gacgattccg ccgccgtgcc gacgccgcga aacgggcggc ggccgaatag agggcccagg
     8401 aataaatcat acttattcgc gcgattggac ctggctcgca tcacagtatg cgctttggca
     8461 agccggcagc ggcgtctccc cacctggcat ctcagaccgc ccgcgccacg gatctcatca
     8521 agatgagaaa ggacggcgcg gaaggcgggc tgcagttggc gattggacaa gtgcgctggc
     8581 tcctgtatag ggcacgaggt tcaggcatga cccgtcatgg gaaaagggcc ttttgagttt
     8641 tgcagatcag cccctgagga gcggcgcaat gtcgtcaacc ttcgagaaag tcgccgatat
     8701 catcgcggag accgccgata tcgaccggac cagcatcacc gaggcatcgc acgcgatcga
     8761 cgatctcggc atcgattcgc tggcattcct cgacatcgcc tttgccatcg acaaggcttt
     8821 tggcatcaag cttccgctcg aacagtggac gcaggaggtc aacgagggca aggcgccggc
     8881 cgagaactac tttgtcatga agaacctctg cgcccgcatc gacgaactgg tcgccgccaa
     8941 gaaagcgtga tcgatccatc gcgggcaggc ggtcaccgga cccgggcatg aagcttgaat
     9001 atttccagat gatcgaccgc gtggtctccc tcgaccggga gacgctgcgg cttgtcgcaa
     9061 cgtcgcgcat tccttcgatc agcccgattt tcgaggggca ttttcccgga catccgctga
     9121 tgcccggcgt gctgctgatc gaaaccatgg cgcagacctc gggctatctg ctgctcgggc
     9181 tgaacaattt caccgccatg ccgttcctga ccaaggtggt gaacgcgaac ctgagacggt
     9241 tcctgggacc cgacacgccg atcgaaatgg aagccacgct tctgcacgac ggttcaggct
     9301 atgccgtcac gaaagccaag atcaccacag gcggcaaggc agtctgcgac gccgagatca
     9361 tgttcaagat cctgcccttc ccggaggacc ttggcgcgaa aatgcgcgcc aatgcggcca
     9421 gcctcggcat ggttgtgacg gagccggcag catgagcggg cgtgacgttg tcattaccgg
     9481 cgtcgggctg gtctcgagcc tcggtgaggg gctggaagcc catggtgccg cgctggccga
     9541 cgcggggcac gtgcatgtcg acgcgacgac ctacgcgcct tacgccgttc atccggccac
     9601 cgcgcttgaa cttgaccggc agatccccaa gaagctcgac cagaagcaga tggaaccgtg
     9661 gcagcggctc ggcacctatg cggccgggct tgcactcgag atggctggcc tgaagggcgg
     9721 caaggatctt ctgggcgaga cccagctgat cgtcgcggcc ggcggcggcg agcgtgatta
     9781 cgcggtcgac agccagattc tgtcgcagat gcctggggcg gaaaagccag aggttctgct
     9841 caatgaaaag ctgatgagcg aactcaggcc gacgctgttc ctggcgcaat tgtccaacct
     9901 gctcgctggc aacatttcta tcgtgcatgg cgtcactggc gcctcgcgta ccttcatggg
     9961 cgaagagcaa tgcggtgtgg atgccttccg catcgctttc gagcgcatcc gcgccggaca
    10021 gggcgaccgg ttcctcgtcg gcggctccta caacgcggag cgtgccgatt cgctgctgat
    10081 cttcgcgatg ggcggattcc tgcgccgcgc cggctacagg ccggtcgatg cgcgcgacga
    10141 tgatcccggc ttcattctcg gcagcggcgg cgctttcctg atgctggaag cacgggaagc
    10201 cgccgaagcg cggggagcga ccattctggc caggctcgac agcgtggcgg cgatgcgcac
    10261 gcggcgcaag cccggcgccg tccaggcggc cctgggtaaa gcattgcaga acgcgtcggc
    10321 ctcatccggc ggctcgccgc tggtgatctc cggagcaacc ggggttgccg ggctcacagc
    10381 agaagaactt gccgccatcg gctccgccct gccgggcgcc ccggtgcgga ttgccggcaa
    10441 ccgcgttggc catgtgctgg aagcgcattt tccggtttcg ctggcgttgg ccgttgccgc
    10501 gcttcggcag ggggcggtct atccgccgac gccgggcagc gcggtcgagg cgccgtatgc
    10561 gggcgcgccg gattgcgtga tcatcaccag cgtcgggcac atgcgcggcg aaggtctcgc
    10621 cgttgtggcg gcggaacagg gggcggcatg aaggaccgtt tcaatcgccc tgtcgtcgtc
    10681 gtcaccggca tgggcatggt cacctcgctg gggcgcggca aggccgacaa ttgggcggca
    10741 ctcaccgccg gccggtcggg cattcggcgc atttcccggt ttgcggtcga gggtttgaaa
    10801 acaaccatcg ccggcaccgt cgatttcgtg ccggcagagc cattctgtgc agcgcaactg
    10861 tcggagaatt ttgcccgcct tgccgccgag gaagctattg gcgaagccgc aatcgggatg
    10921 aagggcgatt tccccgggcc gctgttcgtc gccgtgccgc cggtcgagat ggaatggccg
    10981 cagcgagtag acctggcacg cgcctccggt gccggcagcg aagtcaccta caccgacctg
    11041 ttgcgtgcgg cgggcaccgg acacttcaag ccctggcacg atctgttcct gttcggcacg
    11101 gtcgccgatc gactggctga tgacttcggc accaaaggct cgccgatctc gctgtcgacc
    11161 gcctgttcgt ccggcgccac cgccatccag ctcggcgtcg aggccatccg tcgcggcgag
    11221 accgaggccg ccctgtgcat cggcaccgat gggtcggtgc aggccgaggc actgatccgc
    11281 ttttcgctgc tgtcggcgct gtcgacccag aacgatccgc ccgagggcgc gtccaaaccg
    11341 ttttccaaga accgcgacgg ttttgtcatg ggcgagggcg cggccgcgct tgtgctcgaa
    11401 tcctatgcct ccgccaaggc gcgcggcgca cgcattctcg ggattctctc cggctgcggc
    11461 gagaagggcg acgggttcca ccgcacccgc accagcccgg acggcatgcc ggcaatccgt
    11521 gccatgcgcg acgcgatcgc cgatgccggc ctgacgccgg acgacatcga ttatgtcaac
    11581 gcccacggta cctcgacgcc cgagaacgac aagatggaat gcctcggctg catgacagtg
    11641 atgggcgagc ggatgcggag cgtgccgatt tcctccaaca agtcgatgat cggccacacg
    11701 ctgacggcgg ccggcgcagt cgaggcggtg atttcgctga tgacgctggc caaccagcgc
    11761 attccgccga cgatcaatca tcatctgccc gatccggcga tcccgcttga tgtcgtgccg
    11821 aatgcagcgc gcgatgcacg ggtacgccat gtgatgtcga attccttcgg ctttggcggt
    11881 cagaacacgg tgctgctgct ctccggtgag gtgccagcat gagcgggcgg gtgctggtca
    11941 ccggcggcgg caagggcgtc ggcgcggcca tcgtgcgggc gctggccgaa gccggtcatg
    12001 atgtcgattt cacctatcgc acgtcctctg ccgcagccga ggcgctggcg gcggagatcg
    12061 ccgcagccca tcccggccgg accttgcgcg cactgccgct tgatcttgcc gaccgcgagg
    12121 cggtggaacg gttttcgacc gaaatcgagg atacggtcta ttttggcttc gtccacaatg
    12181 ccggccagtc ctatgacgcg cttgcggcga tgatggcgca ggacaaggcc gaagcgatca
    12241 tgcaggtcaa tttctggtcg atgacgcgcc ttgccaaggc ggttctgcgg ccgatgctgc
    12301 gggcgcggtc gggccggatt gtcgggatcg gttcggtggc ggcgctcgaa ggcaatcagg
    12361 gcaatgccgc ctatgccgcc accaagggag cgatgctgag ctatatgcgc acgctcgcgg
    12421 tggagagcgc caagcgtggt gtcaccgtca attatatcgc gcccggtttc atcgacaccg
    12481 acatgctggc accctatgcc aattaccggg cacagatgga aaaacagatc ccggccgggc
    12541 ggttcgcgca gccggacgac atcgccgcgc tggtgacttt tctgctctca cccggcgccg
    12601 cctacatcac cggcgcggtg ctgccggtgg atggcgggct gaccgcaacg attgccttcc
    12661 agcggtaacc agcgcctgcc cggtcatccc gagcttgtcg aaggatgccg ttccaacgta
    12721 tcagcagctt ggttcggcag gttcatgatg atgcccgcag gacaaacagg atggacccat
    12781 gcgcgcgttg caacttttcg gcgatcgtga ccttcggctg accaggctgg agccaccgcc
    12841 tcaccccggc cggggcgagg tacaggtgcg ggtccatgcg gtcggcctca accacatcga
    12901 cgtgtggggg ttccgcggca tggcgtttgc caagcgcaag atgccgctga tcgttggcgc
    12961 ggaagctgcc ggcgtcgtcg cctcggtggg cgaggatgtc acccatgtcg atatcggcga
    13021 ccgggtggtg atgtatggtg cgcgcacctg cgggcattgc acgatgtgcg tcaaggggcg
    13081 cgacaacctg tgcgagaacg tcggcggcat catgggcttc cacatcgacg gcttcgcctg
    13141 cgacttcgtc aacatgccgc agcggctgct ggtcaaggtg ccggacgggg tcagctttgc
    13201 ggaggcggcc tgcgcgccga tcgccttcgg cacggtgcag cacatgctgt tcgacaacgc
    13261 caggcttgag ccgggcgaga ccatccttgt gcaggccggc ggctccggta tcggcacggc
    13321 ggcaatccgc atggccaagg caatcggctg cacggtcatc accacggtgg gcgacgacga
    13381 caaggcggcg aaagccaagg cgctcggcgc cgatcatgtc atcaattacc gcgaggagcg
    13441 cttcgagggc gtcgtgcgca agatcaccgg ccgtaagggt gtcgatgtgg tgttcgagca
    13501 taccggcgtc gatacctggc agggctcact cttgtcgatg aagaagggcg ggcggctggt
    13561 cacctgcggt tcgacctcgg gcatttcgac aacgatgaac ctgatgcaat tgttccagca
    13621 gcaatacaag atcttcggct cgttcggttg ctccatccgc aacatcgaac aatcgctgga
    13681 gaagatggcg cagggcatcc gcccggtgat cgacaccgaa ttcccccttg ccgatttcgc
    13741 caaggggctc gacaggctcg aaggccgcaa ggttttcggc aagatcatcg tttcgttttg
    13801 atccccctcg tctggaaaat ccgcgcctgg cgcgcgctca ataccgccgg cggctggctg
    13861 atgctgccgc tggtgcggct ggtattcggc tttctgaagc tgctcgggcc ggaccgggcc
    13921 gccgacctcg gcggctggct gacacgcagc atcggcccgc gtctgaaggt gcaccgggtc
    13981 ggccttgcga acctgcgcgc cgcctatccc gacaagaccg aggacgagat cgcggccatc
    14041 gcgctgggcg cctgggacaa tctcggccgt accggtgccg aatatgccca tctgggctca
    14101 ttgttcgatt gggatgccga caatccggat ggcccgagcc ggacggaagt ttcgggaatc
    14161 gagcatttcg aggcgctgcg tgacgatggc cggccgggca tcatcttctc tgctcatctc
    14221 gccaactggg aattgccggc gatctgcgcg gcaaaatatg gccttcaggt caccgccgtt
    14281 ttccgcgcgc cgaacaatcc ggcggtcaac cgggttgtcc acgaaatccg ctccggcacg
    14341 atgggcgggc tggaggcggc gcgcgccggg gcggcgttcg ccatgaagga ggccctggaa
    14401 aagggcgggc atctcggcat gctgatcgat cagcatttca cccgcggcgt gccagtgacg
    14461 ttcttcggcc gtacggcgat g
//
LOCUS       DAOMXG010000049        14316 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_2329_length_14316_cov_9.397448, whole genome shotgun sequence.
ACCESSION   DAOMXG010000049 DAOMXG010000000
VERSION     DAOMXG010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 14316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14316
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2329_length_14316_cov_9.397448"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="PLQ11_05035"
     CDS             complement(<1..885)
                     /locus_tag="PLQ11_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRK24301.1"
                     /translation="MTGVPLIRVSGLTKHFGTPPAIVHAVEDVSLAIDRGEVVGLVGE
                     SGSGKSTIGRMILRSIDPTAGEILFEGQDIARMSQRELRPLRRRMQMVFQDPYASLNP
                     RLRVRDIVGEALDAHGLARGAARDRRVGELLETVGLQADHATRFPHEFSGGQRQRIGI
                     ARALAVEPDLIVTDEPVSALDVSVQAQVLNLLQDLRQRFGMALLFISHDLDVIELMCD
                     RIVVLYLGRVMESGPVDEVCRRPRHPYTQALLAASPKPDPDAPRTRRLLQGDMPSPLA
                     PPSGCVFRTRCPLATAACA"
     gene            complement(882..1877)
                     /locus_tag="PLQ11_05040"
     CDS             complement(882..1877)
                     /locus_tag="PLQ11_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018444640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRK24302.1"
                     /translation="MAGLLDIASLEVRFRTEAGEVTPVSGVSLTVEAGRTTALVGESG
                     SGKSVTSLAVMGLLPRPSGRISGGSVHFAMKSGGAVDLASLPPAEMRKLRGSEIAMIF
                     QEPMTSLNPVLTVGEQIAEQVRLHMGLGSAAAMRRAEDMLGLVEIPDARRRATDYPHQ
                     MSGGMRQRVMIALAMSCNPRLLIADEPTTALDVTIQAQILALIDKLRREHDMGVLFIT
                     HNLGVVAEVADAVTVMYAGEVVESADVRTLFRAPRHPYTRALIDCLPARAAAGPGGRR
                     IVHSIPGTVASAASAQGCRFAPRCNHATAPCHAAPPALERNEAAHLIRCARWRDL"
     gene            complement(1877..2725)
                     /locus_tag="PLQ11_05045"
     CDS             complement(1877..2725)
                     /locus_tag="PLQ11_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRK24303.1"
                     /translation="MDLAGIVLFFRRLFRRKLVLFATLVLALIVVLAVLAPLVAPYSP
                     TGMRVANRLKPPSGINWFGTDEFGRDVLSRIIYGGRASLGIGMAVVIASMLAGTTLGL
                     LAGYFRTLDGIIMRMVDAMMSLPDILLAIFLVAVLGASAGNVVLALAIVYTPRVVRVV
                     RGSTLVVRELPFVEAARTIGVSTWRILTRHILLNVASPILVQATFIFAYAVLAEAGLS
                     FLGAGVPPEIPTWGTMIATGQQYADQAVWVVLFPGLAIVLSALCLQLLGDGLRDMLDP
                     KLRKAL"
     gene            complement(2733..3674)
                     /locus_tag="PLQ11_05050"
     CDS             complement(2733..3674)
                     /locus_tag="PLQ11_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018448592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRK24304.1"
                     /translation="MLVAIARRLAGMLAVMFIVATMVFFLTRLAPGDPASLMLGDQAT
                     AADVAVLRATYGLDQPLLVQYVLWLKEIASGNLGQSIFLQRPVLQAIAERAEPTFFLA
                     LFSILIAALIGIPSGIASAVWRGDRLDQAVSAGAMLAASIPSFWLGLLLIQFLSVRLG
                     WFPVAGYGDPGASLGMRLYHLVLPATVLGIVNSALITRFTRASMLDVLSEDYIRTARA
                     KGISEARIVLRHALGNALVPIITVVGLTAALLVGGAIITETVFGLPGIGNLVVSAVLR
                     RDYPVIQGALIVIAGIYVIINFAIDVIYTLVDPRVRT"
     gene            complement(3776..5347)
                     /locus_tag="PLQ11_05055"
     CDS             complement(3776..5347)
                     /locus_tag="PLQ11_05055"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRK24305.1"
                     /translation="MSDQARDMSLHGWSRRDLAKFIAGAGLLATLPAGSQALAQGAKS
                     ANLAMIGEPQSLDPMASTADLVSTIMMHVYESLYTFDKDLNAQPMLAAAMPKVSADGK
                     LYEIEIRKGVKLHSGRDLDVEDVIASLNRWIAMTPRGKSVGANVDKLEAKGSHSLAIS
                     LKNPVPALLTHMALPSGYAAIMAKESIANPLVSFVGTGPYKFQERKPDQYVILTKFDG
                     YSARSEPANGYAGKREAKIAELRFIPVPNANTRVEGVVAGQYHFADQLPVESYKRIAA
                     TASVKPVLVMPYGFPYFVLNTKQGPFADKKIRQAYQLALNNTEMMLAAFGDDKFFKAT
                     AAHFPKASPFHSEAGGAAYNKGDAAAAKKLLAEAKYDGSPIRILNSKQYEFHNRMALV
                     MAENLKEAGFKVQLDVVDWATLVQRRADPAVYDVYVTHSTFLPEPMLSPPQLGDGAPG
                     WWDTPAKKATLAAFNAESDPVKRGALWAKVQEVVYDEVPYIRVGDFAALSATSAKMTG
                     LVPMPSPAFWNVDLA"
     gene            5654..6832
                     /locus_tag="PLQ11_05060"
     CDS             5654..6832
                     /locus_tag="PLQ11_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HRK24306.1"
                     /translation="MNHSVVLKGGRVIDPSQGIDGIMDVAFRDGKVVASGVGLSTGAG
                     TEVIDVAGKIVTPGLIDLHTHVYDRGTSLGVDAMRLFQRGAVTTLVDTGSAGPGNFPG
                     FRSHVIERIPVRVLAYLNVSFAGIYGFSKRIMVGEASDMRLMAPIDTVEVANANRDMI
                     IGIKVRVGRNASGFSGLAPLQIARQVADELGLPLMVHIDEPPPMLHEVLALLRKGDVL
                     THCFRPFPNCASTADGKVHDYVLDARRRGVIFDIGHGMGSFSFQVARTMLANAFQPDC
                     ISSDVHALCIEGPAFDLLTTMSKFLCLAMPLNDVIRAATEAPAAALNRPDLGTFRPGA
                     AGDASVLSLESGSFDYVDSVGERLKGGQRLTASGVVLAGQYQPCTPEIGLGGRPHSLA
                     "
     gene            6840..7265
                     /locus_tag="PLQ11_05065"
     CDS             6840..7265
                     /locus_tag="PLQ11_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018429798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HRK24307.1"
                     /translation="MTYASLFFASFVAATILPAQSEAVLAALLVRGEQPAAALIIVAT
                     IGNVLGSLCNWLLGRGIDRFRQHRWFPATPEQLDRAAAHYARWGHWSLLLSWAPVIGD
                     PLTLIAGVLREPLWRFLLLVTLAKLGRYLIVYFAVQALA"
     gene            complement(7279..8139)
                     /locus_tag="PLQ11_05070"
     CDS             complement(7279..8139)
                     /locus_tag="PLQ11_05070"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019962819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HRK24308.1"
                     /translation="MAGWSQTWTFVDGRWLEGNPPLTGPRSHAFWLASSVFDGGRVFD
                     GVGPDLDLHFQRLNRSGPAIGLKATHSVEEMQALTWEGVKKFSPGTAIYVKPMYWAEG
                     EGASAILPDAETTRFCLCLFEAPMATAYGGASITLSPFRRPTIESAPTDSKAGCLYPQ
                     NGRALREARARGFSNALVRDMLGNIAETCTANVFMARDGVVFTPIPNGSFLNGITRQR
                     SIALMRADGMEVVEKALTYADFQGADEIFTSGNYSKIVAVTKIDDRDLQPGPLTRRAR
                     DLYMDYAHSR"
     gene            complement(8220..8612)
                     /locus_tag="PLQ11_05075"
     CDS             complement(8220..8612)
                     /locus_tag="PLQ11_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017483717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HRK24309.1"
                     /translation="MEQLIGRVAAAAGIDAGLAEKAVGMIFGFLKKEAPADSFAALVN
                     AVPGAAAVAEANSGGGGGGGLMGSLMGMMGGGGGVMGLGAQMMGAGLSMGQIQSISKE
                     VFAYGREVAGEDTMGQIVGAIPGLSQFV"
     gene            8795..10342
                     /locus_tag="PLQ11_05080"
     CDS             8795..10342
                     /locus_tag="PLQ11_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015952612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HRK24310.1"
                     /translation="MASPLIAPAGTILVGKSVKPEVLTLKLANRHGLITGATGTGKTV
                     TLQTMAEEFSRQGVPVFAADIKGDLSGIAKPGRGEAHFLKRCEDIGLDYKADQSPVVF
                     WDVFGQQGHPIRATVAEMGPLLLSRMLMLNEVQEGVLNILFRYCDDNKLLLLDLKDLR
                     AMLAEFGSNRELQGELMATYGNITPQTVGAIQRQLLILENQGAARFFGEPALDILDLI
                     KTEGGRGVVNILAADKLMEAPQLYATFLLWLLSELFQELPEVGDLDKPRLVFFFDEAH
                     LLFTDAPKALLAKIEQVVRLIRSKGVGVYFVTQNPLDVPDSVRAQLGNRVQHALRAFT
                     PRDQKAVKAAAETFRQNPKFNTETAITELAVGEALVSMLEGKGVPSMVERTLIKPPTG
                     RVGPVTPAERSELLAVSPMKGAYDEMVDNESAYEILQKRVEQATTKATEGGMMDTIKD
                     TVGGWFGGSADKPKTGPGSRGGRVPPSMAEKVITSAARSAATSVGRQIGTAIFRSVLG
                     SILKGGR"
     gene            10345..11751
                     /locus_tag="PLQ11_05085"
     CDS             10345..11751
                     /locus_tag="PLQ11_05085"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012605815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HRK24311.1"
                     /translation="MGLDRVLDRIDADVPKALERLFALIRIPSISTDSAYAKDVVACA
                     AALKADVDSLGFQTTIHPTAGHPVVVAHRPKTGAPHVLFYGHYDVQPVDPEALWTEPP
                     FAPSVKEIAPGRKVITGRGSADDKGQVMTFIEACRAFLATTGDLPVGVTLLIEGEEES
                     GSPNLPAFVETHRKQLQADIGLVCDTGMWDADTPAITTSLRGLVYQEIVVRCADRDLH
                     SGLYGGAAANPAHVLARIITSLHDRNGRVRIPGFYKGVTNPPAAQLANWKSLGLTEAE
                     FLGPIGLKHSVGEKGRELIEHIQSRPTCDVNGIWGGYTGEGAKTVIAAEARAKISFRL
                     VGQQDPKAIAKAFAAYVKRQVPKDCSVEVIPMKGSRAFEVDYNAPAIAAARTALRQEW
                     GREAAAIGSGGSIPIVGDFKRILGLDCVMVGFGLEDDRVHSPNEKYDLKSFQKGTRSW
                     ARILDALARMKPAKEPAR"
     gene            11751..11969
                     /locus_tag="PLQ11_05090"
     CDS             11751..11969
                     /locus_tag="PLQ11_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2093 domain-containing protein"
                     /protein_id="HRK24312.1"
                     /translation="MKSLFERPEKRGLEAQLHYLDGDYRILRHGSFVRCAVTGQPIPL
                     DELRYWSVKRQEAYATPEASLTRHRGQG"
     gene            complement(11905..12954)
                     /gene="lpxK"
                     /locus_tag="PLQ11_05095"
     CDS             complement(11905..12954)
                     /gene="lpxK"
                     /locus_tag="PLQ11_05095"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018263112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HRK24313.1"
                     /translation="MKAPTFWWDRTPGAIARLLMPFGWLYGTITTIRMKRAGYRAPVP
                     VLCIGNFTAGGAGKTPTTIALAEAMAAGGEFPVILTRGYGGTARGVTVVVPAIHDART
                     VGDEALLLARIAPTVVASDRVAGAEAAARAGASVILMDDGLQNPSLAKDVSIAVVDGA
                     VGFGNGLCLPAGPLRAPVEQQAPLVDALVAIGMGHGLTQAVAAGSVSGRPVFRAELGV
                     SDAVAEQFAGKRVLAFAGIGRPEKFFDTLMSLYVKIEIARPFGDHHAYSDAEAEELMA
                     TAAARGLALVTTSKDHVRLAGSPALERLKAATEPLPVQLTLPDGLIALVRDRISLARG
                     GGSAKLQASHRPPDA"
     gene            complement(12951..14234)
                     /locus_tag="PLQ11_05100"
     CDS             complement(12951..14234)
                     /locus_tag="PLQ11_05100"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HRK24314.1"
                     /translation="MATAALSLYRLVTRAAEPLVPLLLKRRIANGKEEVTRINERLGY
                     PERPRPPGPLVWLHGASIGETLSLMPLVSALGERGLTILLTSGTVTSAEIAASRLPAG
                     AIHQYLPVDTPGAARRFIAHWRPNVALFAESELWPNLILTAKAAGVKLAIVNGRMSER
                     SAKSWQRLPKSIARVLGAFDLCLAQSGADAERFSRLGAANARFIGNLKFDGPAPHADA
                     AMLADLQQRIGTRPVLVAASTHPGEDEVVLAAHLAAEMVHPGLITVLAPRHPQRGDDI
                     ERLARSAGLNPARRSRQELPDQATRIYIADTVGELGLFYRLGPLAVLGGSFIAHGGQN
                     PIEPAKLGVGVLHGPHVFNFTDVYAAFDAAGGAELVADPDRLGHRIADLLGDSAGLGR
                     MQKAAEAVAAELSGALGRTLEALEPLLPRVAEQSA"
BASE COUNT         2597 a         4612 c         4604 g         2503 t
ORIGIN      
        1 ggcacatgca gccgttgcca gcggacagcg ggtccggaac acgcagcccg aaggcggggc
       61 cagcggcgag ggcatgtcgc cctggagcag ccggcgtgtg cgcggcgcat cggggtctgg
      121 cttgggagag gcagccagca gcgcttgcgt gtagggatgg cgcgggcggc ggcagacctc
      181 gtccaccggg ccgctttcca ttacccggcc gagatagagc acgacgatcc ggtcgcacat
      241 cagctcgatc acatcgagat catggctgat gaacagcaac gccatgccga accgttgccg
      301 caagtcctgc aacaggttca gcacctgtgc ctgcaccgag acatcgagcg cggagaccgg
      361 ttcatcggtg acgatcaggt ctggctcgac cgccagggcg cgggcgatgc cgatgcgctg
      421 acgctggccg cctgaaaatt catgtggaaa ccgggtggca tggtctgcct gcagcccgac
      481 cgtttccagc aattcgccca cccgcctgtc gcgtgcggcg ccgcgggcca gcccatgcgc
      541 atccagtgcc tccccgacga tatcgcgcac ccgcagccgc gggttgaggc tggcataggg
      601 atcctgaaac accatctgca tgcgtcgtcg cagcggacgc agctcccgct gtgacatgcg
      661 ggcgatatcc tgcccctcga aaaggatctc gccggccgtc ggatcaatcg atctgaggat
      721 catccggccg atggtgctct tgcccgagcc ggattcgcca acaaggccga cgacttcacc
      781 acggtcgatg gcgagcgaaa catcctccac cgcatgaaca attgccggcg gcgtgccgaa
      841 atgcttggtc aggccggaga cgcggatcag gggcacgccg gtcacagatc cctccatcgc
      901 gcacagcgga tcagatgcgc cgcctcgttc cgctccagcg ccgggggagc ggcatggcag
      961 ggagcggtgg catgattgca gcgcggcgca aacctgcagc cttgggccga agcggcgctg
     1021 gcgaccgtgc cggggatcga gtgaacgatg cgccgtccgc ccggacccgc cgccgcacgg
     1081 gcgggcaggc agtcgatcag ggcccttgtg taaggatggc gcggagcccg aaacagggtg
     1141 cggacatcgg ccgattccac cacctcgccc gcatacatca cggtgacggc atcggctact
     1201 tcggccacca caccgaggtt gtgagtgatg aacaacacgc ccatgtcatg ctcgcggcgc
     1261 agcttgtcga tgagcgcgag gatctgcgcc tgaatggtga catccagcgc ggtggtcggc
     1321 tcgtcggcga tcaggaggcg cggattgcac gacattgcca gcgcgatcat cacccgctgg
     1381 cgcatgccgc cggacatctg gtgcggatag tcggtcgccc ggcggcgggc atcaggaatt
     1441 tccaccagac ccagcatgtc ctcggcgcgg cgcatggctg cggcagagcc cagccccatg
     1501 tgcaggcgga cctgttcggc aatctgctcg ccgaccgtca gcaccggatt gagcgacgtc
     1561 atcggctcct ggaagatcat ggctatctcg ctgccgcgca gcttgcgcat ctccgccggt
     1621 ggcaggctgg cgaggtcaac cgcgccgccg gatttcatgg caaaatgcac cgatccgccg
     1681 ctgatccgcc ccgacgggcg cggcagcagc cccatgacgg cgagactcgt caccgatttt
     1741 cccgagcccg attctcccac cagcgcggtt gtccggcctg cctcgaccgt aagactgacg
     1801 ccgcttacag gcgtaacctc gcccgcctcg gtgcggaagc gaacctcaag gctggcaatg
     1861 tcgagaaggc cagccatcac agcgccttcc gcagtttggg atcaagcatg tcccgcaatc
     1921 cgtcgcccag aagctgaaga cacagcgccg acagcacgat ggcaagcccg ggaaacagca
     1981 ccacccagac cgcctgatcg gcatattgct gaccggtggc gatcatcgtg ccccaggtgg
     2041 ggatttccgg cgggacgccg gcgccgagga acgacagccc ggcttccgcc agcaccgcgt
     2101 aggcgaagat gaaggtggcc tgcaccagga tgggcgaggc gacattgagc aggatgtggc
     2161 gggtcaggat gcgccaggtg gaaacgccga tggtgcgcgc cgcctcgaca aagggaagct
     2221 cgcgcaccac cagcgtggag ccgcgcacga cccgcaccac acgcggtgtg tagacgatgg
     2281 ccagcgccag cacgacattg ccggcagatg cgcccagcac cgccaccaga aagatcgcca
     2341 gcaggatatc aggcaacgac atcatcgcat cgaccatgcg catgatgatc ccatcaagcg
     2401 tgcggaagta gccggccagc agaccgagcg tcgtgcccgc cagcatggat gcgatgacaa
     2461 ccgccatgcc gattcccaat gacgcgcgac cgccatagat gatgcgggac agaacatcgc
     2521 ggccgaattc gtcggtgccg aaccagttga tgccgcttgg cggtttcagc cggttggcga
     2581 cacgcatgcc cgtcggcgag tagggcgcga ccagaggcgc aagaaccgcc agcacgacaa
     2641 tcagcgccag cacaagcgtg gcgaacagca ccagcttgcg ccggaacagc cggcggaaga
     2701 acaagacgat tccggccagg tccatggtca gctcaagtcc tgacgcgcgg atcgacgagc
     2761 gtgtagatca cgtcgatagc gaagttgatg atgacataga tgccggcgat gacgatcagc
     2821 gcgccctgga tgaccggata atcgcgccgc agcacggcgg acaccacaag gttgccgatg
     2881 ccgggcaggc caaacaccgt ttcggtgatg atcgccccgc ccaccaagag cgcggccgtc
     2941 agaccaacga cggtgatgat cggcaccaga gcattgccaa gggcgtgacg caggacaatg
     3001 cgggcctccg aaatgccctt tgcccgcgcg gtgcggatgt agtcctcgct cagcacgtcc
     3061 agcatactgg ctcgggtgaa acgggtgatc agcgccgaat tgacgatgcc gagcaccgtt
     3121 gccggcagca ccagatgata gagacgcatg cccagcgatg ccccgggatc gccatagccg
     3181 gcaaccggaa accagccgag ccgcaccgac aggaactgga tcagcagcag cccaagccag
     3241 aaactcggga tgctggcagc cagcatggcc ccggcgctga ccgcctgatc cagccggtcg
     3301 ccgcgccaca ccgccgaggc gatccccgac ggaatgccga tcagcgccgc aatgagaatg
     3361 gaaaacagcg ccagaaagaa cgtcggttcg gcgcgttcgg caatcgcctg caacaccggg
     3421 cgctggagaa aaatcgactg gccaagattg cccgaggcaa tctccttcag ccacaggaca
     3481 tattgcacca gcaggggctg atcgagccca taggttgcgc gcagcaccgc gacatcggcc
     3541 gccgtcgcct ggtcgccaag catcagggac gcagggtcgc ccggcgcaag gcgcgtcagg
     3601 aaaaacacca tcgtggcgac gatgaacatg acggccagca ttccggccag cctacgggcg
     3661 atggcgacga gcatgcatcc tcacggaaac tcagatgcgg tgcacgaggc cgcagcggca
     3721 ggcgccgcgg ccctgtgggt tcacactgct ggactgccgc tggccggcgc gatggttagg
     3781 ccagatcgac gttccagaag gcgggcgatg gcatcggcac caggccggtc atcttggccg
     3841 aagtggcgct gagcgcggca aaatcgccga cccggatgta gggcacctcg tcgtagacga
     3901 cttcctgcac cttggcccag agcgcgccgc gcttgaccgg atcgctttcg gcgttgaagg
     3961 ccgccagtgt cgccttcttg gccggcgtgt cccaccagcc cggagcgcca tcgcccaatt
     4021 gcggcggcga cagcatcggc tcgggcagga aggtcgaatg tgtgacgtag acgtcataga
     4081 ctgccggatc ggcgcggcgc tgcaccagcg tcgcccagtc caccacatca agctgcacct
     4141 tgaagcccgc ttccttcaga ttctcggcca tgaccagcgc catgcgattg tggaactcat
     4201 actgcttgga attgaggatg cggatcggcg atccgtcata tttggcctcg gccagcagct
     4261 tcttggccgc tgccgcatcc cccttgttgt aggccgcccc gcctgcttcc gaatggaacg
     4321 gcgaagcctt ggggaaatgc gcggcggtcg ccttgaagaa cttgtcgtcg ccaaatgccg
     4381 ccagcatcat ctcggtattg ttgagagcaa gctgataggc ctgccggatt ttcttgtcgg
     4441 cgaacgggcc ctgcttggtg ttcagcacga aatagggaaa gccatagggc atcaccagta
     4501 ccggcttgac cgaggccgtg gcagcgatgc gcttgtagct ctccaccggt aactggtcgg
     4561 caaagtgata ttgcccggcc acgacacctt ccacccgggt gttggcgttc ggcaccggga
     4621 tgaagcgcaa ttcggcaatc ttcgcctcgc gcttgccggc atagccgttg gccggttcgc
     4681 tgcgcgccga atagccatcg aacttggtca ggatcacata ttggtccggc ttgcgctcct
     4741 ggaacttgta ggggccggtg ccgacaaagc tgacgagcgg attggcgatc gattccttgg
     4801 ccatgattgc ggcatagccg gaaggcagcg ccatatgggt gagcagggca gggacaggat
     4861 ttttgagtga aatcgccagg ctgtgcgagc ccttggcttc aagcttgtca acattggcgc
     4921 cgacggactt gccacgcggc gtcatggcga tccagcggtt gagcgaggcg atgacgtcct
     4981 cgacatcaag gtcgcgaccc gagtgcagct tcacgccctt gcggatctcg atctcgtaca
     5041 gcttgccgtc ggcgctgacc ttcggcatcg ccgctgccag catcggctgg gcgttgagat
     5101 ccttgtcgaa cgtataaagc gattcataga catgcatcat gatcgtcgag acgagatcgg
     5161 cagtggacgc catcgggtcg agcgactgcg gctcgccgat cattgccaga ttggcgctct
     5221 tggcgccctg ggcaagcgcc tggcttccgg cgggaagcgt tgccagcagg ccggcgccgg
     5281 cgatgaactt ggcgagatcg cggcggctcc agccgtgcag cgacatgtcc ctggcttgat
     5341 cagacatcgg caaccctccc gtgagcgccg tccggaagtc ggggcgcggt tcttggttta
     5401 gattttgaaa ctttattgct tgacgttggg ctgtcaagcg ctaatgccgg ggctctgggg
     5461 taatgtgaac ttagaatagc gcgcgttgat gcggttattg cgctgcaaaa atagcatctt
     5521 gtggcgctga gcactggatt cgaggtctgt cctgccgcac aaaatgaaac gttatttctt
     5581 ttttgattga tctgtcgctg cgcttttgta tccttgcccg gaaagatcgc agcccgggcc
     5641 cggaggcata gccatgaacc attccgttgt cttgaagggc ggtcgcgtca ttgatccgtc
     5701 gcaggggatc gatggcatca tggatgttgc gttccgtgat ggaaaggtgg tggcgagtgg
     5761 cgtcgggcta tcgacaggtg ccgggacaga agtgatcgat gttgcgggca agatcgtcac
     5821 gcccgggctg attgatctgc acacccatgt ctatgacagg ggaacatcgc tcggtgtgga
     5881 cgccatgcga ttgttccagc gcggcgcggt gaccaccctg gttgataccg gaagcgccgg
     5941 tccgggcaat tttccggggt tccgttcgca tgtgatcgag cgcattccgg tccgcgttct
     6001 ggcctatctc aacgtgtcct ttgccgggat ctatggcttt tcgaagcgga tcatggtggg
     6061 cgaagccagt gacatgcggc tgatggctcc gatcgatacg gtcgaggtcg ccaatgccaa
     6121 cagggacatg atcatcggca tcaaggtgcg cgtcggccgc aatgccagcg ggttctcggg
     6181 gctggcgccg ctgcagatcg cccggcaggt ggcagatgaa cttgggctcc cgctgatggt
     6241 ccatattgat gagccgccgc cgatgctgca cgaggttctc gcgctgttac gcaagggcga
     6301 tgtgctgacc cattgcttcc ggccattccc caattgcgct tcgacggcgg atggcaaggt
     6361 gcatgattat gtgctcgatg cccggcggcg cggcgtgatt ttcgacattg gccatggaat
     6421 gggatcgttc tccttccagg tcgcgcggac catgctggcc aatgccttcc agcccgattg
     6481 catttcctcg gatgtgcatg cgctgtgcat cgaggggccg gcgttcgacc tgctgacgac
     6541 gatgtcaaaa ttcctctgcc tcgccatgcc gctgaacgat gtcatccgcg ccgcgaccga
     6601 ggcgccggca gcggcattga accgcccgga tctcggcaca ttccggcccg gggccgccgg
     6661 ggatgcctct gtcctgtcgc tcgaatcggg cagtttcgac tatgtcgatt cggtcggaga
     6721 acgcctgaaa ggcggccagc gcctgacggc aagcggcgtc gttctggcgg gacaatatca
     6781 gccctgcacg ccggagatcg gtcttggcgg acggccgcac agtctggcat gacggggcga
     6841 tgacctatgc cagtctgttc tttgcgtcat ttgtcgccgc gaccatcctg cccgcccaat
     6901 cggaagcagt gctggcggcg ctgctggtca ggggtgaaca gccggctgcg gcactgatca
     6961 tagtggcaac gattggcaat gtgctgggca gcctgtgcaa ctggctgctg gggcgcggca
     7021 tcgaccgctt caggcagcac cgctggtttc cggccacgcc cgaacagctc gaccgcgccg
     7081 cagcccatta tgcccgctgg ggccattggt cactgctgct gagttgggca ccggtgatcg
     7141 gcgatccgct gaccctgatt gccggcgtgc tgcgcgaacc attgtggcgc tttctgctgc
     7201 tggtcacgct tgccaagctc ggccgctatc tgatcgtcta tttcgccgtc caggcattgg
     7261 cttgaaggcg cgcccggttc agcgcgaatg ggcatagtcc atgtaaagat cgcgcgcccg
     7321 gcgtgtcagc ggtcccggct gcaggtcgcg gtcgtcgatc ttggtcaccg cgacgatctt
     7381 ggagtaattg cctgaggtga agatctcgtc ggcgccctgg aaatcggcat aggtgagcgc
     7441 cttttccacc acttccatgc catcggcgcg catcagcgcg atgctgcgct ggcgggtaat
     7501 cccgttaagg aacgacccgt tggggatcgg cgtgaacacc acgccgtccc tggccatgaa
     7561 cacatttgcc gtgcaggtct cggcgatatt gcccagcatg tcgcgcacca gcgcattgga
     7621 aaagccgcgc gcgcgggcct cgcgcagcgc ccggccgttc tgtggataga ggcacccggc
     7681 cttcgaatcg gttggtgcac tctcgatagt cggccggcgg aacggcgaca gcgtgatcga
     7741 tgccccgcca taggctgtgg ccatcggcgc ctcgaacagg cacaggcaga accgggtcgt
     7801 ctccgcatcc ggcaggatcg cgctggcgcc ttccccctcg gcccagtaca tcggcttgac
     7861 gtagatcgcg gtgccgggag aaaacttctt caccccctcc caggtcaggg cctgcatctc
     7921 ctcgaccgaa tgggtcgcct tgagcccgat cgccgggccc gagcggttga gccgctggaa
     7981 atgcaggtca agatcggggc cgactccgtc gaataccctg cctccatcga aaacgctcga
     8041 agccagccag aaggcgtgac tgcgcggtcc ggtgagcggg ggatttccct ccagccagcg
     8101 gccatccacg aatgtccagg tttgcgacca gcccgccatg cgctcactcc catgcgatca
     8161 gttgacctgt atgggaacag gaggcggcgc ccccggtcaa gggcgccgcg cactttgcct
     8221 cagacgaact gcgacaggcc cggaatggcg ccaacgatct ggcccatcgt gtcctcgccc
     8281 gccacttcac ggccgtaggc gaacacttcc ttggagatcg actggatctg gcccattgac
     8341 agaccggcgc ccatcatctg cgcgccaagc cccatgacgc cgccgccgcc gcccatcatg
     8401 cccatcagcg agcccatcag gccgccgcca ccgccgccgc cgctgttggc ttccgccacc
     8461 gccgccgcac ccggaactgc attgaccagc gccgcgaaac tgtcagccgg cgcttccttc
     8521 ttgaggaagc cgaaaatcat gccgacggcc ttttcggcca gaccggcgtc gatgcctgcc
     8581 gctgcagcga cacgtccgat gagttgttcc atttatctct cccgtttcgg cagtccttgc
     8641 cgtcgcccca tacataatac acagcagcag cggcctgcaa ggagggatag ttcatatatg
     8701 aacaaaattg accgccctgc aaaggaactg gttttcgcag ccctctgcgc ctataaggct
     8761 ggccagcttg gccaattttg agagacggca gaccatggct tcaccgctga tcgcacccgc
     8821 aggcaccatt ctggttggca agagtgtcaa gccggaggtt ctgaccctca agctggccaa
     8881 tcgtcatggc ctgatcaccg gggcgaccgg caccggaaag accgtgacct tgcagaccat
     8941 ggccgaggag ttttcgcgtc agggcgtgcc ggtctttgcc gccgatatca agggcgacct
     9001 ttcgggaatt gccaagccgg gccgtggcga ggcgcatttc ctcaagcgct gcgaggatat
     9061 cggcctcgac tacaaggccg accagtcgcc tgtggtgttc tgggatgtgt tcggccagca
     9121 gggccatccg atccgcgcca ccgtggccga gatggggccg ctgctgctct cgcgcatgct
     9181 gatgctgaac gaagtgcagg aaggcgtcct caacatcctg ttccgctatt gcgacgacaa
     9241 caagctgctg ctgctcgacc tcaaggacct cagggcgatg ctggccgaat tcggctcgaa
     9301 ccgcgaattg cagggcgagt tgatggccac ctacggcaac atcacgccac agacggtggg
     9361 ggcgatccag cgccagctgc ttattctgga aaatcagggc gccgcgcgct tcttcggcga
     9421 gccggcgctt gatatcctcg atctcatcaa gaccgagggc ggccgcggcg tcgtcaacat
     9481 tctggcggcc gacaagctga tggaagcccc gcagctttat gcgaccttcc tgctctggct
     9541 gctttccgaa ctttttcagg agctgcccga ggtcggtgat ctcgacaagc ccaggctggt
     9601 gttcttcttc gatgaggcgc atctgctgtt caccgatgcg ccgaaagcgc tgctggccaa
     9661 gatcgaacag gtcgtccggc tcatccgctc caagggcgtc ggcgtctatt tcgtcaccca
     9721 gaacccgctc gatgtgccgg attcggtgcg cgcccagctt ggcaatcgtg tgcagcatgc
     9781 cctgcgcgcc ttcacgccgc gtgatcagaa ggcggtgaag gccgcagctg aaacattccg
     9841 ccagaatccg aaattcaaca ccgagacggc gatcaccgaa ctggcggtgg gcgaagcgct
     9901 ggtctcgatg ctggaaggca agggcgtgcc ttcgatggtc gagcgcacgc tgatcaagcc
     9961 gccgacgggg cgcgtcggcc cggtcacccc tgccgagcgc tccgagcttc tcgccgtgag
    10021 cccgatgaag ggcgcctatg acgagatggt cgacaacgag agcgcctacg agatcctgca
    10081 gaagcgcgtc gagcaagcca cgacgaaggc caccgaaggc ggcatgatgg acaccatcaa
    10141 ggataccgtc ggcggctggt tcggcggttc ggcagacaag ccgaaaaccg ggcccggctc
    10201 gcgcggcggg cgggtgccgc catcgatggc ggaaaaggtg atcacctctg cggcgcgctc
    10261 cgctgcaact tcggtcggcc gccagatcgg cacggcgatc ttccgctcgg tgcttggctc
    10321 gattctcaaa gggggacgtt gagcatgggg ctggacaggg ttcttgaccg gatcgatgcc
    10381 gatgtgccca aggcactgga gcgattgttc gcgctgatcc gcattccctc gatctcgacg
    10441 gactcagcct atgccaaaga tgtcgtggcc tgtgcggcgg cgctcaaggc ggatgtcgac
    10501 agccttggct ttcagacgac cattcatccg accgccggtc atccggtggt tgtcgcccac
    10561 cggccgaaaa cgggtgcgcc gcatgtcttg ttctacggcc attatgacgt gcagccggtc
    10621 gatcccgagg ccctctggac cgagccgccg ttcgctccct cggtgaagga gatcgcgccg
    10681 ggtcgcaagg tcatcaccgg gcgtggttct gccgatgaca agggccaggt gatgaccttc
    10741 atcgaggcgt gccgcgcctt tctggccacc acgggtgacc tgccggtcgg tgtcactctg
    10801 ctgatcgagg gcgaggagga aagcggctcg cccaacctgc ctgccttcgt cgagacccat
    10861 cgcaagcaat tgcaggccga catcggtctg gtctgcgata ccggcatgtg ggatgccgac
    10921 acgccagcca tcaccacctc gctgcgcggc ctcgtctatc aggaaatcgt ggtgcgctgc
    10981 gccgatcgcg atctgcattc tgggttgtat ggtggggctg ccgccaatcc ggcgcatgtg
    11041 ctggcgcgga tcatcacctc cttgcatgac cgcaacggtc gggtccggat tccgggcttc
    11101 tacaagggcg tcaccaatcc gccggccgcc cagctggcca actggaaatc gcttggcctg
    11161 accgaggctg aatttctcgg accgatcggc ctcaagcatt cggtgggcga gaaggggcgt
    11221 gagctgatcg agcacatcca gtcgcgcccg acctgcgacg tcaacggcat ctggggtggt
    11281 tataccggcg agggcgccaa gacggtgatc gccgccgaag cgcgcgccaa gatttccttc
    11341 cgcctggtcg gacagcagga ccccaaggcg attgcgaagg cttttgccgc ctatgtgaag
    11401 cggcaggtgc cgaaggattg ttcggtcgag gtgatcccga tgaagggctc gcgcgccttc
    11461 gaggtcgatt acaatgcgcc ggccattgcc gccgcccgga cggcattgcg gcaggaatgg
    11521 gggcgtgagg cggcggccat cggctcgggc ggatcgattc ccatcgtcgg cgatttcaaa
    11581 cggatcctcg ggcttgattg tgtgatggtc ggtttcggcc ttgaggacga ccgggtgcat
    11641 tcgcccaacg agaaatatga tctcaagagc ttccagaaag gcacccgctc ctgggcgcgc
    11701 attcttgatg ccctggctcg gatgaagcct gccaaggagc cggcgcgatg atgaaatccc
    11761 tgttcgagcg ccccgagaaa cgcggcctgg aagcacagct tcactatctt gatggcgact
    11821 atcgcatcct gcgccatggc agctttgtcc gctgcgcggt gaccgggcag ccgatcccgc
    11881 tcgacgaatt gcgctattgg agcgtcaagc gtcaggaggc ctatgcgacg cctgaagctt
    11941 cgctgacccg ccaccgcggg caaggctgat cctgtcgcgc accagggcga tcagcccgtc
    12001 aggcagtgtc aactgcaccg gcaggggctc ggtggccgct ttcagccgtt caagcgccgg
    12061 gcttcccgcc agccggacgt gatccttgct ggtggtgacc agcgccagcc cgcgcgcggc
    12121 ggcggttgcc atcagttcct cggcttcggc atcgctatag gcatggtgat cgccgaacgg
    12181 gcgggcgatc tcgatcttca cataaagcga catcagcgtg tcaaagaact tttccggtcg
    12241 cccgatgccg gcaaaagcga gcacgcgctt gccggcgaat tgctcggcaa ccgcgtcgct
    12301 gacgccgagt tcggcgcgga acaccggcct gccgctgaca ctgccggcgg ccaccgcctg
    12361 ggtcagacca tggcccatgc cgatggcgac cagcgcgtca accagtggcg cctgctgctc
    12421 caccggtgcc cgcaggggac cggcgggcag gcaaagcccg ttgccgaatc ccaccgcccc
    12481 gtccaccacg gcgatcgaga catccttggc gagcgacgga ttttgcaacc cgtcatccat
    12541 caggatcacg ctcgccccgg cccttgccgc agcctccgcg ccggcaacgc ggtcggatgc
    12601 cacgacggta ggcgcgatgc gggcgagcag cagcgcttca tcacccaccg tccgcgcatc
    12661 atggatggcc ggcaccacga cggtcacgcc ccttgccgtg ccgccgtagc cgcgcgtcag
    12721 gatgaccgga aattcgccgc cggcggccat tgcttcggcg agcgcaatcg tcgtcggggt
    12781 tttcccggcg ccgccagcgg tgaaattgcc gatgcagagg accggcacgg gcgccctgta
    12841 accggcccgc ttcatccgga tggttgtgat cgttccgtag agccagccga acggcatcag
    12901 caggcgggcg attgctcccg gcgtccggtc ccaccagaac gtcggggctt tcatgcactt
    12961 tgctccgcaa ccctgggcag cagcggctca agcgcctcca gtgtccgtcc aagcgctccg
    13021 ctcaactccg cagcgaccgc ttccgccgcc ttctgcatcc ggccaagtcc ggcgctgtcg
    13081 ccgagaaggt ccgcaatgcg atgccccagc cggtccggat cggccaccag ttcggcgccc
    13141 ccggcggcat cgaaggcggc atagacatcg gtgaagttga acacatgcgg cccgtgcagc
    13201 acgccaacac cgagcttggc cggctcgatc gggttctgcc cgccatgggc gatgaacgag
    13261 ccacccagca ccgccagcgg cccgagcctg tagaacaacc ccagttcgcc aaccgtgtcg
    13321 gcgatgtaga tccgtgtggc ctggtccggt agctcctgac gcgagcgcct tgccggattg
    13381 agcccggcgc tgcgggcgag ccgttcgatg tcgtcgccac gctgcgggtg acgcggagcc
    13441 aggacagtga tcaggcccgg atgaaccatt tctgcggcaa gatgggctgc cagcaccact
    13501 tcatcctcgc cgggatgggt gctggcggca accagcaccg gccgtgtgcc gatccgttgt
    13561 tgcagatccg ccagcatcgc ggcatcggca tgcggcgccg gcccgtcaaa cttgagattg
    13621 ccgataaagc gtgcgttcgc agcgccaagc cgggaaaagc gctctgcatc cgccccgctc
    13681 tgcgccaggc agagatcgaa ggcgccgaga acgcgcgcga tgctcttggg caggcgctgc
    13741 catgatttcg ccgagcgctc cgacatccgc ccgttgacga tggcaagctt aacgcctgcc
    13801 gctttcgccg tcaggatcag gttcggccac aattcgcttt cggcaaacag cgccacattc
    13861 ggccgccaat gggcgatgaa ccgtcgtgcg gcaccgggcg tgtcgaccgg caggtattga
    13921 tggatcgcgc ccgccggcag gcggctggcg gcaatttcgg cggaggtgac ggtgcccgac
    13981 gtcagcagga tcgtaaggcc gcgttcgccg agggcggaaa ccagcggcat cagcgacagg
    14041 gtctctccga tcgaggcacc atgcagccag accagcggac cgggcgggcg tggcctttcg
    14101 ggatatccca accgttcgtt gatgcgggtg acttcctctt tgccgttggc gatgcgccgc
    14161 ttcagcagca acggcaccag cggttcggcg gctcgggtaa cgagtcggta gagcgacaaa
    14221 gcggccgtcg ccatctcagg cctcgacagg gtctgtacgg gctgcggccc gtgccgccgc
    14281 cacgctgtcg cgcccggcgc cgggatcggt cgcccc
//
LOCUS       DAOMXG010000050        14144 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_2359_length_14144_cov_10.379800, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000050 DAOMXG010000000
VERSION     DAOMXG010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 14144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14144
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2359_length_14144_cov_10.379800"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="PLQ11_05105"
     CDS             complement(<1..253)
                     /locus_tag="PLQ11_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HRK24315.1"
                     /translation="MLRFSRLKILGVVALALASILYSLPSGLPQSTRDAIERSIPGWV
                     PKWIVPYKGITLGLDLQGGTHVVLEVDQPALIRTMVQQLQ"
     gene            complement(283..612)
                     /gene="yajC"
                     /locus_tag="PLQ11_05110"
     CDS             complement(283..612)
                     /gene="yajC"
                     /locus_tag="PLQ11_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HRK24316.1"
                     /translation="MVITPAFAQAAAAPGGGELIAQFAPFVLILIIMYFLLIRPQQKR
                     AREHQEMIKNVRRGDTIIMSSGMIAKITKVVDDSEVEVEIADGVRVRMMRAMIADVRT
                     KGEPVAG"
     gene            complement(739..1422)
                     /locus_tag="PLQ11_05115"
     CDS             complement(739..1422)
                     /locus_tag="PLQ11_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012452927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRK24317.1"
                     /translation="MMIPALALRTIERTYFQAGEALEILRGADLTVQPGELVALVAPS
                     GTGKSTLLHCAGLLERPDGGEVLIGGMPTRSLPDNERTRLRRTEIGFVYQFHHLLPEL
                     TAIENVMMPQLIAGLGREQARSRSAELLTFIGLGKRLDHQPAKLSGGEQQRVAIARAV
                     ANAPRLLLADEPTGNLDPGTSDHVFSMLTAIVKQSGLAALVATHNLDLARRMDRRVTI
                     RDGHIAELE"
     gene            complement(1419..2714)
                     /locus_tag="PLQ11_05120"
     CDS             complement(1419..2714)
                     /locus_tag="PLQ11_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011068949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HRK24318.1"
                     /translation="MTAAVSLKDTAGGWLKRVASSFEVMLAWRYLRARRREGFISLIA
                     LLSFLGIMLGVATLIVVMSVMNGFRKELIGKILGVNGHAFIQPLGEPLQDYEAIARRI
                     EGVKGVTIAAPMIEGQVLAASQTASTGALVRGLSEADIRKFKVVSGNIREGSLDGFSD
                     GNGIAIGKRMAGHLNVTIGDKVRLLSPKGPSTPFGMAPRQKAYEVTAIFEVGMSEFDA
                     GFVFMPIGESQIYFDLEGQASVIEVFIQDPEKIDDLKPAIQQALGPVATVTDWRFRNR
                     VFFGALEMERTMMFIILMMIVLVAAFNIIAVLIMMVKDKTRSIAILRTMGATRGSVLR
                     IFIMTGAIIGVVGTILGVLLGLLISANVESLRQFFSWLLARPLFPPELYYLNRMPSVI
                     DPTEISIIVGVTLLLSFAATLYPAWKAAKLDPVEALRGE"
     gene            complement(2711..4033)
                     /locus_tag="PLQ11_05125"
     CDS             complement(2711..4033)
                     /locus_tag="PLQ11_05125"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HRK24319.1"
                     /translation="MRLTRFFLPTLRETPKEAEIVSHRLMLRAGMIRQESAGIYAWLP
                     LGLRVLNKVCAIIREEQNRSGAIELLMPTIQSADLWRESGRYDAYGKEMLRIKDRHER
                     DMLFGPTNEEMITEIFRAYVRSYKDLPLNLYHLQWKFRDEVRPRFGVMRSREFLMKDA
                     YSFDLDQAGARHAYNKMFVAYLRTFERLGLKSIPMRADTGPIGGDLSHEFIVLASTGE
                     SEVFCHKDYLDFAVPPADTDFDDIKGIQATVDKWTSLYAATSEMHDAAAYEAIPADRR
                     VSARGIEVGHIFYFGTKYSEPMRAVVNGPDGKEHMVHMGSYGIGPSRVAAAAIEASHD
                     EAGIIWPEAIAPFRVALLNLRQGDSACDAACEALYRDLTARGVDVLYDDREERPGGKF
                     ANADLIGLPWQVMVGPKSLAEGKVETKHRASGARDLMSPADVIARLTA"
     gene            complement(4524..4754)
                     /locus_tag="PLQ11_05130"
     CDS             complement(4524..4754)
                     /locus_tag="PLQ11_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1467 family protein"
                     /protein_id="HRK24320.1"
                     /translation="MKFGTWLAIYFIVWWVMLFAALPFAVRRDAEPDEVVAGSDPGAP
                     ARPNLKRALLITTSLAAIITAAFWWLINNYWV"
     gene            complement(4754..5158)
                     /gene="mce"
                     /locus_tag="PLQ11_05135"
     CDS             complement(4754..5158)
                     /gene="mce"
                     /locus_tag="PLQ11_05135"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HRK24321.1"
                     /translation="MIGRLNHVAIAVRDLAAATRTYAGALGAKVSAPQALPEHGVTVV
                     FVELPNTKVELLEPLGASSPIARFLEKNPDGGMHHICYEVTDILAARDALKAAGARVI
                     GDGTPKIGAHGKPVLFLHPKDFNGTLVELEQV"
     gene            complement(5169..6833)
                     /locus_tag="PLQ11_05140"
     CDS             complement(5169..6833)
                     /locus_tag="PLQ11_05140"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HRK24322.1"
                     /translation="MADPAELVFVPLGGLGEIGMNAALYGYGPPRRREWLLVDLGVAF
                     AGEDMPGVDLIMPALGFIEQQKERLAGIVLTHAHEDHFGAVADLWPRLGVPLYATQFA
                     ADLLEARRLSEKGAPKVPIRRIGQATRFSVGPFDLETVPVAHSIPESCALILRTPAGT
                     VVHTGDWKIDPTPVLGLPTDEARFRAIGDEGVLALVCDSTNILREGESASERQVADAL
                     KEIIAGSPGRVLVTTFASNVARIRAVAEAAAACGRQVIIAGRAMERVAEIAAELGMFS
                     GLPPFRGPDAYQSLPRDKVVVLVTGSQGEPRAALARIAAGEYPDVRLASGDRVIFSSR
                     AIPGNEKAINRIINALARQDIEIITDRHGLVHVSGHPRRDEVRRMYEWVRPKIAIPAH
                     GEALHLTEHRAFARSMGVDHAIRAFNGDLVRLAPGDPVIVQEVPHGRLVKDGMLITEE
                     TGAVAERRKLAYVGIVTVAFAIDERGEFASDPEIETTGIPETTADGTVIAELALDAVE
                     AAVRSLGKARRRDVSAVQEAVRRAVRSAIADVWDKKPVCQVFVLDV"
     gene            complement(6826..7602)
                     /locus_tag="PLQ11_05145"
     CDS             complement(6826..7602)
                     /locus_tag="PLQ11_05145"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HRK24323.1"
                     /translation="MRLGGAATTAGYSLVSLQSVDSTNRLALDLAQQAGADRTWVVSE
                     EQRAGRGRQGRTWASPPGNFYGSLLLVAPCPTRDLPKLGFVAGVALADTVRSLSLRGP
                     VHLKWPNDLMAGNAKLAGMLLEGRAMADGRMAIAIGIGLNIANAPPVSGRQTTSLGEL
                     GVHADRREVFRGLSDRVAERLDQFAGGTGFALIRDAWTERTLPLGTAISVQLASGLRG
                     GRFAGIDASGQLLLDTAAGRIAVEAGDVLLNASESAFDHG"
     gene            complement(7615..9057)
                     /gene="nuoN"
                     /locus_tag="PLQ11_05150"
     CDS             complement(7615..9057)
                     /gene="nuoN"
                     /locus_tag="PLQ11_05150"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010969150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HRK24324.1"
                     /translation="MTSTLAQVPALGPFLPELVLAVVALVLVLLGAFRGDQCKTLIDR
                     VTIAALVIAGLLVIEQGPGRFLTFNDAFIADSFARFMKVLTLIGSIAAVVLSTGFLRD
                     EKVQRFEYPILILLSTLGMMVMISANEMISLYLGLELQSLALYVLASIHRDNSRASEA
                     GLKYFVLGALSSGMLLYGMSLVYGFTGSISFKAIAASVHGGHGTGVTFGLVFILAGLA
                     FKVSAVPFHMWTPDVYEGAPTPVTAFFATAPKMAAVAILVRVLITAFPAVTRDWQQIV
                     TFMAIASMALGAFAAIPQTNIKRLMAYSSIANMGYGLTGLAAGSIEGVEGIAIFMAIY
                     LAMTLGAFAVILSMKTDKGHVEAIDDLSGLSKSQPFMAAMLMLIMFSLIGIPPLAGFF
                     GKFYVFAAAVKAKLMALAILGILTSVVSAYYYLRIVKVMYFDEPKREFLPVGGEVRLV
                     MTLAGLVVLLFWVFPAPIKSAAEAAAKSLF"
     gene            complement(9082..10590)
                     /locus_tag="PLQ11_05155"
     CDS             complement(9082..10590)
                     /locus_tag="PLQ11_05155"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HRK24325.1"
                     /translation="MFGFGILSGLTFLPLLGAAIILLQRDDEAGWRNTRCIALWTTLA
                     TFLLSLVAWVRFDTANAGFQLLERKAWLSETIVYQLGVDGISMPFVLLTTFLMPLCIL
                     ASWHSINTRLRAYMAAFLVLETMMIGVFVALDVILFYLFFEGGLIPMFLIIGIWGGQR
                     RLYASFKFFLYTLIGSVLMLIAIMAMYWDAGTTDMIKLMMSHKFGGKLQIWLWLAFFA
                     SFAVKMPMWPVHTWLPDAHVEAPTAGSVILAGILLKMGGYGFLRFSLPMFPEASHYFA
                     NMVFWMSCIAIVYTSLVAMMQEDIKKLIAYSSVAHMGFVTMGIFTFNTPGVQGAVFQM
                     VSHGLISGALFLCVGVIYDRMHTREIAAYGGLVEKMPLYAAIFLLFTMANVGLPGTSG
                     FIGEFLTLTGSYKASTWVAFVATTGVILSAAYALWLFARVVFGTMTRPGLMAIPDLDA
                     RELAILLPLAALTIYYGVQPGPILDACAASVDLVIKQATAGLAPVKAALMMH"
     gene            complement(10590..12668)
                     /gene="nuoL"
                     /locus_tag="PLQ11_05160"
     CDS             complement(10590..12668)
                     /gene="nuoL"
                     /locus_tag="PLQ11_05160"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HRK24326.1"
                     /translation="MYTAIVFLPLLGFILAGLASLIAHKPGRDVVAFGAADHSGHDHA
                     HGHDAHGHDDHGHDDHHAPPSPHSAGVIKFAEWITTGFLFVSCLLSWVAFFKIALGPS
                     EGVRVLLGNWMTSGTMSVDWALRIDPLTAVMLVVVTTVSALVHLYSMGYMHEDPSRPR
                     FFAYLSLFTFAMLMLVTADNLAQMFFGWEGVGLASYLLIGFWFHKPSANAAAMKAFIV
                     NRVGDFGFALGIFLVFWTFGTVSLDGIFADVAGKAKDTTGFLGWQVHTITLACCLLFM
                     GAMGKSAQFLLHTWLPDAMEGPTPVSALIHAATMVTAGVFMVARLSPLFEFSPFAQSL
                     VIIVGATTAFFAATVGLVQNDIKRVIAYSTCSQLGYMFVALGVGAYSAGIYHLFTHAF
                     FKALLFLGAGSVIHAMHHEQDMRKMGGLAQKIPFTAWMMIIGTLALTGFPFTAGFFSK
                     DAIIEAAYASHRVGNGYAFVLIVLAALFTSFYSWRLAFMTFYGKTRADKHTYDHAHES
                     PMTMLIPLGVLAAGAIFAGFPFAAKFVGGGFADFFKGSIFMGADNHILHDLHNVPFWV
                     KASPFVMMVLGFVLAWLFYIQDPGRPVRLAETFPGLYRFLLNKWYFDEIYDLIFVRPA
                     KWLGRFFWKKGDGWLIDGFGPDGVSALAGRIAVRAGRVQTGFVYHYAFAMLIGVALIV
                     TWYLYAGGAH"
     gene            complement(12737..13042)
                     /gene="nuoK"
                     /locus_tag="PLQ11_05165"
     CDS             complement(12737..13042)
                     /gene="nuoK"
                     /locus_tag="PLQ11_05165"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HRK24327.1"
                     /translation="MIGLTHYLVLSAILFTLGVFGIFINRKNVIVILMSIELILLSVN
                     INFVAFSSYLGDLTGQVFAMLVLTVAAAEAAIGLAILVVYFRNRGTIAVEDINMMKG"
     gene            complement(13045..13659)
                     /locus_tag="PLQ11_05170"
     CDS             complement(13045..13659)
                     /locus_tag="PLQ11_05170"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HRK24328.1"
                     /translation="MNAVVFFFYLFSTILLASAFMVIAARNPVHAVLFLILTFFNAAG
                     LFILLGAEFLAMLLVVVYVGAVAVLFLFVVMMLDVDFVELRQGFLKYLPIGGLVAVIF
                     GIELALVMGTWKISPEAIATGKAMAQTPVEAMNVRALGRILYTENVYFFQLAGLILLV
                     AMIGAIVLTLRERPGVKRQVVMDQNARTRATAMDVKNVAPGQGV"
     gene            complement(13798..>14144)
                     /gene="nuoI"
                     /locus_tag="PLQ11_05175"
     CDS             complement(13798..>14144)
                     /gene="nuoI"
                     /locus_tag="PLQ11_05175"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HRK24329.1"
                     /translation="EHALRRYPNGEERCIACKLCEAICPAQAITIEAGPRRNDGTRRT
                     VRYDIDMTKCIYCGFCQEACPVDAIVEGPNFEFATETREELFYDKARLLENGARWERE
                     IARSIAMDAPYR"
BASE COUNT         2938 a         4559 c         4401 g         2246 t
ORIGIN      
        1 cctgcaactg ctgcaccatg gtgcggatca gcgccggctg gtcaacctcc agcaccacat
       61 gggtgccgcc ctgcaggtcc agccccaggg tgatgccctt gtagggcacg atccatttcg
      121 ggacccagcc ggggatcgac cgctcgatgg catcacgcgt cgattgcggc aatccactgg
      181 gcagcgaata gagaatgctg gccagtgcca gcgccacgac accgagaatt ttcagccggg
      241 aaaagcgcaa catcgcaatc agtcgccttc agggtgacgg gctcagcccg ccaccggctc
      301 gcccttggtg cggacatcgg cgatcatcgc gcgcatcatg cggacgcgca cgccatcggc
      361 gatctcgacc tcgacctcgc tgtcgtcaac caccttggtg atcttggcga tcatgccgga
      421 cgacatgatg atggtatcgc cgcgacggac attcttgatc atctcctgat gctcgcgcgc
      481 acgcttctgc tgcgggcgga tcagcaggaa atacatgatg atcaggataa gcacgaaggg
      541 agcaaactgg gcgatgagtt cgccgccgcc gggggcggcc gctgcttggg cgaaagccgg
      601 ggtgatcacg atcaatcctc gtctggtcag gccggacggc tgagccttcc ggaatgggca
      661 aattcgcggc ggactataga ggcgcgcacc aagaaggcaa cggctttccg cgccccggtg
      721 ccgccgctgg ccaccatgtc actccagttc ggcgatatgt ccgtcgcgga tggtcacccg
      781 gcgatccatg cggcgggcaa ggtcgagatt gtgggtggca accagcgccg caagcccgga
      841 ttgcttgacg atggccgtca gcatcgaaaa gacatgatcg gaggtgccgg ggtcgagatt
      901 gccggtcggc tcgtccgcca gcagcaggcg cggcgcattg gcaaccgcgc gggcaatggc
      961 gacgcgctgc tgctcgccgc ccgacagttt ggccggctga tgatcgagcc gcttgccgag
     1021 accgatgaag gtcagcagtt ccgccgaacg cgagcgggcc tgttcgcggc caaggccggc
     1081 aatcagctgc ggcatcatca cgttctcgat cgcggtcagt tccggcaaca gatgatggaa
     1141 ctgatagaca aagccgatct cggtgcggcg cagcctcgtc cgctcgttgt cgggcaggct
     1201 gcgggtcggc atgccgccga tcagcacctc gccgccatcc ggcctttcca gcagaccggc
     1261 gcaatgcagc agtgtcgact tgccggtgcc ggaaggcgcc accagcgcca ccagctcgcc
     1321 gggctgcacg gtcagatcgg cgccgcgcag gatctccagc gcctctccgg cctgaaaata
     1381 ggttctctcg atcgtcctga gggcgagtgc cgggatcatc attccccccg caaggcttcg
     1441 accgggtcga gcttggccgc cttccaggcg ggataaagcg tcgcggcaaa ggacaacagc
     1501 aatgtcacgc cgacgatgat gctgatctcg gtcgggtcga tcaccgacgg catccggttg
     1561 agataataaa gctccggcgg gaacagcgga cgcgccagca gccacgaaaa gaactggcgc
     1621 agggattcga cattggccga gatcagcagc cccagcagca ccccgaggat cgtgcccacc
     1681 acaccgatga tggcgccggt catgatgaag atccgcagca ccgatccgcg cgtcgccccc
     1741 atggtgcgca ggatggcgat ggagcgggtc ttgtccttca ccatcatgat cagcacggcg
     1801 atgatgttga aggcggcaac cagcacgatc atcatcagga tgatgaacat catggtgcgt
     1861 tccatttcca gcgcgccgaa aaacacccgg ttgcggaagc gccagtcggt cacggtggcc
     1921 accggcccca gcgcctgctg gatcgcgggt ttcagatcat cgatcttctc cggatcctga
     1981 atgaacacct cgatcaccga tgcctggcct tccagatcga aatagatctg cgattcgccg
     2041 atcggcatga acacgaagcc ggcgtcaaat tcgctcatgc ccacttcgaa gattgccgtg
     2101 acctcatagg ctttctggcg cggcgccatg ccaaatggcg tcgaggggcc cttggggctg
     2161 agcaggcgca ccttgtcgcc gatggtgaca ttgaggtgcc cggccatgcg cttgccgatg
     2221 gcaatgccgt tgccgtcgct gaacccgtcg agcgagcctt cgcggatgtt gccggagaca
     2281 accttgaact tgcggatatc ggcctccgac aggccgcgca ccagcgctcc ggtcgaggcc
     2341 gtctgcgagg cggccagaac ctggccctcg atcatcgggg cggcaatcgt cacgcccttg
     2401 accccctcga tccgcctggc gatcgcttca taatcctgaa gcggctcgcc gagtggctgg
     2461 atgaacgcat ggccgttgac gccaaggatc ttgccgatca actccttgcg aaagccgttc
     2521 atgacgctca tcaccacgat cagcgtcgca acacccagca tgatgccgag gaacgacagc
     2581 agcgcgatca gcgagatgaa cccctcgcgc cggcgggccc gcaggtagcg ccaggccagc
     2641 atcacctcga acgacgaggc aacgcgcttc agccagccgc cggcggtgtc cttcagggag
     2701 acggccgccg tcacgccgtc aaccgcgcaa tcacatcggc cggggacatc aggtcgcgcg
     2761 cgccgctcgc ccgatgcttg gtctcgacct tgccctccgc cagcgatttc ggccccacca
     2821 tcacctgcca gggcaggccg atcagatcgg cattggcgaa tttgccgccc ggacgctcct
     2881 cgcggtcgtc gtagagcaca tcgacgcccc tcgccgtcag atcccggtag agcgcttcgc
     2941 aggcggcatc gcaggcgctg tcgccctgcc tgaggttgag cagcgccacc ctgaacggcg
     3001 caatcgcctc gggccagatg atgccggcct cgtcgtggct ggcctcgatg gcggcggcgg
     3061 caacacggct cggcccgatg ccataagagc ccatatgcac catatgctcc ttgccgtccg
     3121 ggccgttgac caccgcccgc atcggctccg aatatttggt gccgaaatag aagatatggc
     3181 cgacctcgat gccgcgcgcc gagacgcggc ggtccgccgg aatggcctcg taggctgcgg
     3241 cgtcatgcat ctcggacgtt gcggcgtaga gcgaggtcca cttgtcgacc gtcgcctgaa
     3301 tgcccttgat gtcgtcgaaa tcggtgtcgg ccggcggcac ggcgaaatcg agatagtcct
     3361 tgtggcagaa cacctccgat tccccggtcg aggccagcac gatgaactca tgcgaaagat
     3421 cgccgccgat cgggccggta tcggcccgca tcgggatcga cttcaggccg agccgctcga
     3481 aggtgcgcag ataagcgacg aacatcttgt tgtaggcgtg gcgcgcgccg gcctgatcga
     3541 gatcgaagga ataggcatcc ttcatcagga actcgcgcga acgcatcacc ccgaagcgcg
     3601 ggcgcacctc gtcgcggaac ttccattgca gatgatagag attgagcggc aggtccttgt
     3661 aggagcgcac ataggcccgg aagatctcgg tgatcatctc ctcgttggtc gggccgaaca
     3721 gcatgtcgcg ctcgtgccgg tccttgatgc gcagcatctc cttgccatag gcgtcgtaac
     3781 gaccgctctc gcgccacaga tcggccgact ggatggtcgg catcagcagt tcaatggcgc
     3841 cggaacgatt ctgctcctcg cggatgatgg cgcagacctt gttgagcacc cgaagcccca
     3901 gcggcagcca cgcataaatg ccggccgatt cctgccggat catgcccgcg cgcagcatca
     3961 ggcgatgcga gacgatctcc gcctccttgg gcgtttcgcg aagggtcggc agaaaaaacc
     4021 gggtgaggcg catgaaaaga actctcctgg ccacgaatcg ccatcgcggc gtgaacacaa
     4081 gggcctggat gttaagagcg gatcggcccc gaaaattcaa gcgtctctga ctgcgagaga
     4141 aaattcagca ttgcgatgaa attttggcgt gacactgcct attgcctcgg ctaatcaaaa
     4201 ttccgtgaat ggggcaatgc aggcaggagc cgacagaacg ctctgctgcg gtggtccggg
     4261 tctacggagg aagacagcag tcggcgctgg gagaacggat gacaatccgc aggcgccggg
     4321 aagctgacga gagtttctta ttggagcaag gctagccggc cttgcttttt cttttttgcg
     4381 gccctgcttt ttctttttgc tgctttcttg cggcggcctg ctcgctggtt aggagcgaat
     4441 gcgacgccga gcagggccga aggcccggtt gcgagggacg gcccgggaaa tgaccggcag
     4501 gtcatttgtg aaggccactg gtatcacacc cagtagttgt tgatcagcca ccagaatgcg
     4561 gcggtgatga tcgccgccag gctggtcgtg atgagcaatg cgcgcttcag gttcggccgc
     4621 gccggggcgc cgggatcgct gccggcaacc acctcatccg gctccgcatc gcggcgcacc
     4681 gcgaacggca gggcggcaaa cagcatgacc caccagacga tgaaatagat cgcgagccag
     4741 gtgccgaatt tcatcagacc tgttccagtt ctaccagcgt gccgttgaaa tccttgggat
     4801 gcaggaacaa caccggcttg ccgtgcgcac cgattttcgg cgtgccgtcg ccgatgacgc
     4861 gcgcgcccgc cgccttcagc gcatcgcgcg ccgccaggat gtcggtgacc tcgtagcaga
     4921 tgtgatgcat tccgccgtcg gggttcttct caaggaacct ggcgatcggc gatgacgcgc
     4981 ccagaggctc cagcaactcg accttggtgt tgggcagttc gacgaacacc accgtcaccc
     5041 cgtgttccgg cagggcctgc ggcgccgaca ccttcgcgcc caacgcgccg gcataggttc
     5101 tcgttgctgc ggcaagatcg cgcaccgcga tggcaacatg gttcagccgt ccgatcatcc
     5161 ttgcctcctc acacgtccag tacgaatacc tgacagacag gcttcttgtc ccaaacatcg
     5221 gcaatcgccg aacgcactgc gcgccgcacg gcctcctgca cggcggaaac atcgcgccgc
     5281 ctggccttgc cgagcgagcg caccgccgct tccaccgcat caagcgcaag ttcggcgatc
     5341 acagtgccgt ccgctgtcgt ctcggggatg ccggttgtct cgatctccgg atcggaggcg
     5401 aattcgccgc gttcgtcgat ggcgaacgcc acagtgacga tgccgacata ggcaagcttg
     5461 cggcgttcgg cgacggcgcc ggtctcctcg gtgatcagca tcccgtcctt gaccagccgg
     5521 ccgtgcggca cctcctggac aatgaccgga tcgccgggag cgagccgcac cagatcgccg
     5581 ttgaaggcac ggatggcatg gtccaccccc atcgagcggg cgaaggcgcg atgctcggtg
     5641 aggtgcaaag cctcgccatg ggcggggatg gcgatcttcg gccgcaccca ttcatacatg
     5701 cggcgcacct cgtcacggcg cggatggccc gagacatgca caaggccatg ccggtcggtg
     5761 atgatctcga tatcctgccg ggcgagcgcg ttgatgatgc ggttgatcgc cttttcgttg
     5821 ccgggaatgg cacgggagga gaaaatcacc cggtcgccgg acgcaaggcg cacatccgga
     5881 tattcgccgg cggcgatccg ggccagcgca gcccgtggct cgccctggct gccggtgacc
     5941 agcaccacca ccttgtcgcg cggcaggctc tgatacgcat cgggaccgcg gaacggcggc
     6001 aggccggaga acatgccgag ttcggcggcg atctcggcca cccgttccat cgccctgccg
     6061 gcaatgatca cctgccggcc acaggcagca gccgcttccg ccacggcacg gatgcgcgcc
     6121 acattggagg caaaggtggt gaccagcaca cgccccggcg atccggcgat gatttccttc
     6181 aacgcatcgg ccacctgccg ttccgaagcg gattcaccct cgcgcaggat gttggtggaa
     6241 tcgcagacga gcgcaagaac gccctcgtcg ccgatggcgc ggaagcgcgc ctcgtcggtc
     6301 ggcaatccaa gcacaggcgt cgggtcgatc ttccagtcgc cggtgtggac cacggtgccg
     6361 gccggcgtgc gcaggatcag cgcgcaggat tcggggatag aatgggcgac cggaacggtt
     6421 tccagatcga acggaccgac ggaaaagcgc gtcgcctggc cgatgcggcg aatcggcact
     6481 ttcggtgcgc ccttctccga caggcgccgc gcctcgagaa gatcggcggc gaactgtgtc
     6541 gcatagagcg gcacgccgag gcgcggccac agatcggcca ccgcgccgaa gtgatcctca
     6601 tgcgcatggg tcagcacgat gccggcgagc cgctccttct gctgctcgat gaatccaagc
     6661 gccggcatga tcagatcgac gcccggcatg tcctcgccgg caaaagccac gccgagatcg
     6721 accagcagcc attcgcgccg gcgcggcggc ccatagccat agagggcggc attcatgccg
     6781 atctcgccaa gtccgcccag cggcacaaac accagttccg ccggatcagc catggtcgaa
     6841 agcgctctcg ctggcattga gcagcacatc gcccgcctcg acggcgatgc gaccggccgc
     6901 agtatcgagc agcaactggc cggaggcgtc gattccggca aatcgcccgc cgcgcaagcc
     6961 gctggccagc tggaccgaaa tcgccgtgcc aagcggcagg gttcgctccg tccaggcgtc
     7021 acggatcaag gcaaaaccgg tgccgccggc aaactgatcc agccgctcgg caacacggtc
     7081 cgacagcccg cggaagacct cgcggcggtc ggcatgcacg ccgagttcgc cgagcgatgt
     7141 ggtctggcgc ccgctcaccg gcggggcatt ggcgatgttc agcccgatgc cgatggcaat
     7201 ggccatgcgg ccatcggcca tcgcccggcc ctccagcagc atgccggcca gcttggcatt
     7261 gccggccatg agatcattgg gccatttgag atgcaccggc ccgcgcaagg acagggaccg
     7321 gacggtgtcg gccagcgcga cgcccgccac gaacccgagc tttggcagat cccgcgtcgg
     7381 gcatggcgcg accagcagca gcgaaccata gaaattaccg ggcggagagg cccaggtccg
     7441 gccctgccgg ccacggccgg cgcgctgttc ctcggaaact acccaggtcc ggtcggcgcc
     7501 ggcctgctgc gccagatcaa gcgccagccg gttggtgctg tcgaccgact ggagcgatac
     7561 cagtgaatag ccggccgtgg tcgccgcgcc gcccagccgc attgctgctg agcctcagaa
     7621 cagcgacttg gcggcagcct cagccgcaga cttgatcggc gccgggaaca cccagaagag
     7681 cagaaccacc aacccggcca gcgtcatcac cagcctgacc tcgccgccca ccggcaggaa
     7741 ctcccgcttc ggctcatcaa aatacatcac cttgacgatg cgcagatagt aataggcgga
     7801 gacgaccgag gtgagaatgc cgaggatcgc cagcgccatc agcttggctt tgaccgccgc
     7861 tgcgaacacg tagaacttgc cgaagaaacc agccagcggc ggaatgccga tcagcgagaa
     7921 catgatcagc atcagcatgg cggccatgaa gggctgcgac ttggacaggc cggagagatc
     7981 atcgatcgcc tcgacgtggc ccttgtcggt cttcatcgac aggatcacgg caaaggcccc
     8041 cagcgtcatg gccaggtaga tcgccatgaa gatcgcaatc ccctcgacgc cctcgatgct
     8101 gccggcggca aggccggtga gcccgtagcc catgttggcg atcgaggaat aggccatcag
     8161 tcgcttgatg ttggtctgcg gaatggcggc aaacgcgccc agcgccatcg aggcgatggc
     8221 catgaaggtg acaatctgct gccagtcgcg ggtgaccgcc gggaaggcgg tgatcagcac
     8281 ccgcaccaga atggcgaccg ccgccatctt tggcgctgtg gcaaagaacg ccgtcaccgg
     8341 ggtcggcgcg ccttcataaa cgtccggcgt ccacatgtgg aacggcacgg ccgagacctt
     8401 gaaggcgagg cccgccagga tgaacaccag accgaaggtg acgcccgtgc cgtggccgcc
     8461 atgcaccgac gcggcgatcg ccttgaacga aatcgaaccg gtgaagccat agaccaggct
     8521 catgccatag agcagcatgc cggaggacaa ggcgccgagc acgaaatact tcagccccgc
     8581 ctcggaggcg cggctgttgt cgcgatggat cgaggcgagc acatagagcg caaggctctg
     8641 caattcgagg ccgagataga gcgagatcat ctcgttggcc gagatcatca ccatcatgcc
     8701 gagcgtcgag agcaggatca ggatcgggta ttcgaaccgc tgcacctttt catcgcgcag
     8761 gaacccggtc gacagcacca ctgcggcgat cgaaccgatc agcgtcagca ccttcatgaa
     8821 gcgcgcaaag gaatcggcga tgaacgcatc attgaaggtc aggaacctgc caggtccctg
     8881 ctcgatcacc agaagaccgg caataaccag cgctgctatc gtcacccggt cgatcagcgt
     8941 tttgcactgg tcgccgcgga aggcgcccag cagcaccaga accagcgcca caacggccag
     9001 aaccagttcg ggcaggaacg ggcccagcgc cggcacttgt gcaagggtcg aagtcatcgg
     9061 atggatgtcc ttctcattca atcaatgcat catcaaggcg gccttgaccg gagccagtcc
     9121 ggcggtggcc tgcttgataa caaggtcaac ggaagcggcg caggcatcga ggatcggccc
     9181 cggctgcacg ccgtaataga tggtcagcgc cgccagcggc agcaggatgg ccagttcacg
     9241 cgcgtccaga tccggaatgg ccatcagccc gggcctggtc atcgtgccga acaccacgcg
     9301 ggcaaacagc cacagcgcat aggccgccga caggatcacg ccggtcgtcg cgacaaaggc
     9361 cacccaggtc gaggccttgt aggatccggt cagcgtcagg aactcgccga tgaagccgga
     9421 ggtgccgggc aggccgacgt tggccatggt gaacagcagg aagatcgccg cataaagcgg
     9481 catcttctcg accagccctc cataggccgc gatctcgcgg gtgtgcatgc gatcgtagat
     9541 gacgccgacg cagaggaaca gcgcgccgga aatcagcccg tggctgacca tctggaacac
     9601 cgcaccctgc acgcccggcg tgttgaaggt gaagatgccc atggtgacaa agcccatgtg
     9661 cgccaccgag gaataggcga tcagcttctt gatgtcctcc tgcatcatcg ccaccagcga
     9721 ggtgtagacg atggcaatgc agctcatcca gaacaccatg ttggcgaagt aatgcgacgc
     9781 ctcggggaac atcggcagcg aaaaccgcag gaagccgtag ccgcccatct tgagcaggat
     9841 gcccgccagg atgaccgacc cagccgtcgg cgcctcgacg tgcgcgtccg gcagccaggt
     9901 atgcaccggc cacatcggca tcttgaccgc aaacgaggcg aagaacgcca gccacagcca
     9961 gatctgcaac ttgccgccga acttgtggct catcatcagc ttgatcatgt cggtggtgcc
    10021 cgcatcccaa tacatcgcca tgatggcgat cagcatcaga accgagccga tcagcgtgta
    10081 gaggaagaac ttgaacgagg catagagccg gcgctgccca ccccagatgc cgatgatcag
    10141 gaacatcggg atcaggccgc cctcgaagaa caggtagaac aggatgacat cgagcgcgac
    10201 gaacacgccg atcatcatcg tctcgagcac caggaacgcc gccatgtagg cgcgtaaccg
    10261 cgtgttgatc gagtgccagg acgccaggat gcacagcggc atcaggaagg tcgtcagcag
    10321 cacgaagggc atcgaaatgc cgtcaacgcc aagctggtag acaatcgtct cgctcagcca
    10381 ggcctttctt tcaagcagct ggaagccggc attggccgtg tcgaaccgca cccaggccac
    10441 caacgacagc aggaacgtcg ccagcgtggt ccacagcgcg atgcagcgcg tgttgcgcca
    10501 gcccgcctcg tcatccctct gcagcagaat gatcgccgca ccgagcagcg gcaggaaagt
    10561 cagaccggag agaattccga acccgaacat cagtgagctc ctccggcata gaggtaccag
    10621 gtgacgatga gcgccactcc gatcagcatg gcaaaagcgt agtggtagac gaagccggtc
    10681 tgcacccggc ccgcgcgaac cgcgatccgc ccggccagcg ccgagacgcc atccggcccg
    10741 aagccatcga tcagccagcc atcgcccttc ttccagaaga agcggccaag ccacttggcg
    10801 ggacgcacga agatcaggtc atagatctcg tcgaaatacc acttgttgag caggaaccgg
    10861 tagagaccgg ggaaggtctc ggccaaccgc accgggcgcc ccggatcctg gatgtagaac
    10921 agccaggcaa gcacgaagcc gagcaccatc atcacgaacg ggctcgcctt cacccagaac
    10981 ggcacattgt gcagatcatg caggatgtgg ttgtccgccc ccatgaagat cgagcccttg
    11041 aagaaatcgg caaagccgcc gccgacgaat ttcgccgcga acgggaagcc ggcgaaaatc
    11101 gcgcccgccg ccagcacccc gagcgggatc agcatcgtca tcgggctttc atgggcgtga
    11161 tcgtaggtgt gcttgtcggc gcgggtcttg ccatagaagg tcatgaacgc cagccgccac
    11221 gaatagaacg aggtgaacag cgcggccaga acgatcagca cgaaggcata gccgttgccg
    11281 acccggtgcg aggcataggc cgcctcgatg atcgcgtcct tggagaaaaa gccggcggtg
    11341 aacggaaagc cggtcagcgc cagcgtgccg atgatcatca tccaggccgt gaacgggatc
    11401 ttctgggcaa ggccgcccat cttgcgcatg tcctgctcgt gatgcatcgc atggatgacc
    11461 gagccggcgc cgaggaacag cagcgccttg aagaaggcat gcgtgaacag gtggtagatg
    11521 ccggccgaat aggcgccgac gccgagcgcg acgaacatgt agccaagctg cgaacaggtc
    11581 gaataggcga tcacccgctt gatatcgttc tgcaccagac cgaccgtcgc ggcaaagaac
    11641 gccgtcgtcg cgccgacgat gatcaccagc gactgggcaa acggcgagaa ctcgaacagc
    11701 ggcgaaagcc gggccaccat gaacacgccg gcggtcacca tggtcgccgc atggatgagc
    11761 gccgagaccg gcgtcgggcc ttccatcgcg tccggcaacc aggtgtgcag caggaactgc
    11821 gctgatttgc ccatcgcgcc catgaacagc aggcagcagg caagggtgat cgtgtgaacc
    11881 tgccagccga ggaagccggt ggtgtccttc gccttgccgg cgacatcggc gaagattccg
    11941 tcaagcgaaa cggtgccgaa ggtccagaat accaggaaaa taccaagcgc gaagccgaaa
    12001 tcgccgacgc ggttgacgat gaacgccttc atcgccgccg cattggccga gggcttgtgg
    12061 aaccagaagc cgatcaggag atacgaggca aggccgacgc cttcccaacc gaagaacatc
    12121 tgcgccagat tgtccgccgt caccagcatc agcatcgcga aagtgaacag cgacagatag
    12181 gcgaagaagc gcggccgcga cgggtcctca tgcatgtagc ccatcgaata gagatggacg
    12241 agcgccgaga ccgtggtcac caccaccagc atcaccgccg tcagcgggtc gatcctgagc
    12301 gcccagtcga cgctcatggt gcccgaggtc atccagttgc cgagcagaac gcgcacccct
    12361 tccgaggggc cgagcgcgat cttgaaaaac gccacccagg acaagaggca ggagacaaac
    12421 agaaagccgg tggtgatcca ctcggcaaac ttgatcacgc cggcggaatg cggcgagggc
    12481 ggcgcatgat gatcgtcgtg gccatgatcg tcgtggccat gggcatcatg accatgggca
    12541 tggtcgtggc cggaatgatc ggccgcaccg aaggcgacga catcccgccc cggcttgtga
    12601 gcgatcagtg acgcaagacc ggccaggatg aagccgagca gcgggaggaa aacgatggct
    12661 gtatacatgg cttatgcctt tgccgagcgc atgttcttta cgcaaaaccg gggggcaatt
    12721 ctgctaaaca tgcgcgtcag cccttcatca tgttgatgtc ttcaaccgcg atggtgccgc
    12781 ggttgcggaa atagacgacc aggatcgcca gcccgatggc cgcctccgcc gccgcaacgg
    12841 tcagtaccag catcgcgaaa acctgcccgg tgagatcgcc gagataggac gaaaacgcca
    12901 cgaaattgat gttcaccgac agcaggatca gttcgatcga catcaggatg acgatcacgt
    12961 tcttgcggtt gatgaagatg ccgaacacac ccagcgtgaa caggatcgcc gacaggacga
    13021 gataatgcgt gagcccgatc atcgttacac cccctgcccc ggcgcgacat tcttgacgtc
    13081 catggcggtc gcccgcgtgc gcgcgttctg atccatcacc acctgccgct tgacccccgg
    13141 ccgctcgcgc aatgtcagga caatcgcgcc aatcatcgcc accagcagga tcaatccagc
    13201 caactggaag aaatagacgt tctcggtata gaggattcga ccgagcgcgc gcacgttcat
    13261 cgcctcgacc ggggtctggg ccatggcctt gcccgtggca atcgcctccg gcgagatttt
    13321 ccaggtgccc atcaccagcg ccagttcaat gccgaaaatc acggcaacaa gcccgccgat
    13381 cggcagatat ttcagaaagc cctgcctcag ttcgacgaaa tcgacgtcga gcatcatcac
    13441 cacgaacagg aaaagcaccg ccaccgcgcc gacgtagaca accaccagca gcatggcgag
    13501 gaattccgcc ccgagcagga tgaacaaacc tgccgcgttg aagaaggtga ggatcaggaa
    13561 cagcaccgca tgcaccggat tgcgcgcggc aatgaccatg aaggcgctcg ccaacaggat
    13621 tgtcgagaac aggtagaaga agaaaacgac tgcgttcatc ttacggtctc tcgggcggtg
    13681 ccccaccctg ttgcggtctc tctggcggtt ggcggcagac aagccacctg tcggtctata
    13741 tccactttgc tgcgccgcac aaccggcgac gcctcgtcat gacgtctcat ccccacctca
    13801 gcggtagggt gcatccatcg cgatggagcg ggcgatttcc cgttcccagc gcgcgccgtt
    13861 ttcgagcagc cttgccttgt cgtagaacaa ttcctcgcgc gtctcggtgg caaactcgaa
    13921 attcggcccc tcgacaatcg catcgaccgg gcaggcttcc tggcagaagc cgcagtagat
    13981 gcacttggtc atgtcgatgt catagcgcac cgtgcggcgg gtgccgtcgt tgcgccgtgg
    14041 accggcctcg atggtgatcg cctgcgcggg gcagatcgcc tcgcacaatt tgcaggcgat
    14101 gcagcgttcc tcgccgttgg gatagcggcg cagcgcatgc tcgc
//
LOCUS       DAOMXG010000051        13729 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_2458_length_13729_cov_9.233289, whole genome shotgun sequence.
ACCESSION   DAOMXG010000051 DAOMXG010000000
VERSION     DAOMXG010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 13729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13729
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2458_length_13729_cov_9.233289"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="PLQ11_05180"
     CDS             complement(<1..249)
                     /locus_tag="PLQ11_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24330.1"
                     /translation="MVSPLPRVVLVLLVPLALGACNQTSAPPAASAPAGTSSSGTPTV
                     SSNVTPSGFRLPEGAGCSGSVNRFRALMDNDLATGHVAR"
     gene            389..1045
                     /locus_tag="PLQ11_05185"
     CDS             389..1045
                     /locus_tag="PLQ11_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRK24331.1"
                     /translation="MLTIYGVHNSRAARVIWLAYELNITFRQVPIIQAYKLAGRAPGP
                     DPVNTRSPEFLRVNPAGLIPAIDDDGLVLAESLAITLYLARKHGGPVAPQDIGEEGQA
                     MMWSFWAATEIEGHALDIMRNRVMRPPEERDGILADAAEAVLNAKFPVLERALARTGW
                     LVGSRFTVADLNVAEVVRYASAAPALFRTHPNIKKWLDACHQRPAFQRMWTERANEKV
                     "
     gene            1343..2902
                     /locus_tag="PLQ11_05190"
     CDS             1343..2902
                     /locus_tag="PLQ11_05190"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020179204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HRK24332.1"
                     /translation="MTASTNDRVLIFDTTLRDGEQCPGATMTFEEKLEVADLLDELGV
                     DIIEAGFPIASDGDFEAVTAVAKRVKNATVAGLARAGAKDIDRAGEAVKHARRGRIHT
                     FISTSPVHMKWKLQKEPDEVLQLVINSVTRARSLVEDVEWSAEDGTRTEMDFLCRCVE
                     AAIKAGATTINIPDTVGYTTPEEYMTLFQTVRNRVPNSDKAIFSVHCHNDLGMAVANS
                     LAGVKGGARQIECTINGIGERAGNAALEEIVMAIKTRGDVLPYHSRIETTMLNRASKL
                     VAAATSFPVQYNKAIVGRNAFAHESGIHQDGMLKHTQTYEIMTPESVGVSKTSLVMGK
                     HSGRAAFKDKLKQLGYELGENQLEDAFVRFKALSDRKKHIYDEDIEALVDENIATAQD
                     RVKVLSLLVVAGTMGPQLATLTLDIDGQHRTVQSSGNGPVDATFNAIHALLPHEAKLA
                     LYQVHAVTEGTDAQAEVSVRLEDKKSGRLVTGKSADPDTLVASAKAYIVALNKLTNEK
                     SQGFHAQHVSL"
     gene            2979..3785
                     /locus_tag="PLQ11_05195"
     CDS             2979..3785
                     /locus_tag="PLQ11_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24333.1"
                     /translation="MFDAFGLDFRADIVPRVDEWVQGAIIHPGFGHPGDLGSPEPPAG
                     EPTVMLLANQNTFRCGVVLGVSVADEQAMLSSASIFFEGGRQHQLALRRILVSKDRTM
                     GLLDGDLDDAVAITLFEPTFASERQWHGEGSVHEVLVYGICYRLSVGAPPPMQVADPL
                     DGRSGKTQTLYFDDAAMLIPREHPPWLYTIIGPVLQVDDYEMTVLDQHVLKVRVVVAR
                     LDDDKDVDVDLFVTKTALRGGKRPKVGDMIEASVALCARLWEPNVAFSAE"
     gene            3867..3942
                     /locus_tag="PLQ11_05200"
     tRNA            3867..3942
                     /locus_tag="PLQ11_05200"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3900..3902,aa:Lys,seq:ctt)
     gene            4111..5178
                     /locus_tag="PLQ11_05205"
     CDS             4111..5178
                     /locus_tag="PLQ11_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24334.1"
                     /translation="MKRASKRKIADSDADARAGGEPGDTLEAARAKADLSWDLAVPLV
                     THPLMLINIVKAFFFSGLIMALLLSFLIGITGSPEQIPMIIGISFAAAGGIGIVGTAA
                     AALIYRNRMNMHFLLNDRGARAIITDTTARNVSILTIILGVLAGKPGAVGTGLITLSD
                     RDRHAGWRGVVNVRYHPKHHAISLANSWRTVLILFCTPENYEKVAAYVAAATARAKVP
                     ASAKINPIPKLLMHTVGVVLACVPMFVFDYPFRIDIFAPLLILCFALAMLWLIPIMAY
                     VVWAGLAYVVLHTIMKLMETRTSQFGGGLYRAFEVLSGDDIALLVATGIGMAYLIWLS
                     RGLMSGRVRSALGGDMEELDG"
     gene            complement(5256..5549)
                     /locus_tag="PLQ11_05210"
     CDS             complement(5256..5549)
                     /locus_tag="PLQ11_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24335.1"
                     /translation="MKFRTLMLIAAAGMTVLASLPAAAQYSPAAACRSRCEGILDRSN
                     NVNSGALTRRYNACMAQCNQRQNAENAYRRCMANARGNQAAIQNCRNNYQRDR"
     gene            complement(5681..7201)
                     /locus_tag="PLQ11_05215"
     CDS             complement(5681..7201)
                     /locus_tag="PLQ11_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019966122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA synthase"
                     /protein_id="HRK24336.1"
                     /translation="MANHLFDTIVARIKAKDAAFVMLPDGRSYSYGDMLDLSARYANV
                     LAGLAVKPGDRVAVQVEKSIEAVMVYLACVRAGAVFLPLNTAYTAAEVEYFLTDAEPA
                     VFVSDPGKTAALAPVAAKAGARLETLGVANDGTLAEAARAAAPSFANVVRGPDDLGAI
                     LYTSGTTGRSKGAMLSHDNLASNALTLVDYWRFTNKDVLIHALPIYHTHGLFVATNVV
                     LLAGASMHFLPRLDPDTMLKLMATSTAMMGVPTFYTRLLQHPGLTREATKNMRLFVSG
                     SAPLLAETHREWQERTGHAILERYGMTETNMNTSNPYDGDRIAGTVGLPLPGVSVRVA
                     NPETGEILTRPGDIGVLEVKGPNVFKGYWRMPEKTEAEFRKDGFFITGDLGLIDERGY
                     VQIVGRGKDLVITGGFNVYPKEVETEIDSIPGVIESAVIGVPHRDFGEGVTAVVVRKA
                     GAPLTEAGVLAHLAERLAKFKQPKRVIFVDDLPRNTMGKVQKNVLRDSYKDLYAAG"
     gene            7396..8706
                     /locus_tag="PLQ11_05220"
     CDS             7396..8706
                     /locus_tag="PLQ11_05220"
                     /inference="COORDINATES: protein motif:HMM:NF009558.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine
                     deacetylase/succinyl-diaminopimelate desuccinylase family
                     protein"
                     /protein_id="HRK24337.1"
                     /translation="MPGHDLLLPPAATIARVFAEIDARRDAVAELTAELIRFTTVNPP
                     GRDYEPCAEFIAERLANSGFSAEFVRGRGEAGDSHDFPRANVICRREGTGEGPCVHFN
                     SHIDVVEAGHGWTVEPFDGVIRDGRVYGRGACDMKGGLAASIIAVEAIVAAGVPYRGA
                     LEISGTADEESGGFGGVGYLAKRGYFSPPRVDHVIIPEPLNPDRVCLGHRGVWWAEVE
                     MRGRIGHGSMPFLGVSAIRGMADFMARIEADLYPMLDGRRTAMPCVPPGARASTLNYN
                     SIHGGQPEPEDGLPAPVVADWCRLVLDRRYLIEERLDQVKAEIIGLLDATKAARAGLD
                     YSIRELMHFAPVMTEAGDPVVKALDHWIETLFDRPAAHVASPGTYDQKHIQRFGALRS
                     CVAYGPGILDLAHQPDEWVGIDDMVRSAKVMAAAALTLTGISPG"
     gene            8758..10008
                     /locus_tag="PLQ11_05225"
     CDS             8758..10008
                     /locus_tag="PLQ11_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HRK24338.1"
                     /translation="MDETDLLRRLGVALAIGLLVGLERGWHTREEPDSMRPAGLRTFA
                     LTGLLGGIAGALSLKLGGVVLAASFATLSAGFIAFHWLEAQKEGDLGATTVVAGMLTF
                     LLGSYALVGEVTNAVIAGVSMTILLASRQRLHTMVASLTPEEIRAILTLVAMSFLLLP
                     LLPNRPVDPWGAINPYLIWLMAITIALVSFGGYVAVKVFGQERGVLLTAVAGGLASST
                     AVTLSFARLAREHPQSERLLASGILASGATMMVRVAVLAGVLNAKLITPLALPLGAAA
                     LVMLFGGWLLLGGARDDEQPRLDVTNPLELGTALKLAGLILIIMLAGKLMKAQFGDAG
                     VMALAAVSGIADVDAITLSMAQSGGKDLTLAVAAAAILLAVAVNSLAKVGLAGWEGGT
                     RLGFKTGTVSLLAIAVAGAVAYFL"
     gene            10066..11034
                     /locus_tag="PLQ11_05230"
     CDS             10066..11034
                     /locus_tag="PLQ11_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018238534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HRK24339.1"
                     /translation="MRIVATAFALALGLSPAIAQPYPNKPISLVVPFAAGGPTDLIAR
                     LVGESMTKTLGQQIIVENIAGAGGTIGPSRVARAKPDGYTFLVHHVGLTTAATLYRKL
                     DFDIRTAFQPVGIITNGPMILIAKSDFEPKDSAELVAFLRKNGEKVTFATAGLGSGSN
                     LCGMLLEAALKVKLTTVPYRGTGPVMQDLRGKQVDMSCDQATNATQPVRENFVKAYAV
                     TAPARLGPLPNIPTTGENPDLKSLDLNIWNALFAPKGTPPEIVSKVAEALRIALKEEK
                     VVTRFKDLSTDPATPEQATPEALAKAFTAEIERWKPLIEASGQYAD"
     gene            complement(11273..11902)
                     /locus_tag="PLQ11_05235"
     CDS             complement(11273..11902)
                     /locus_tag="PLQ11_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like cysteine peptidase"
                     /protein_id="HRK24340.1"
                     /translation="MAVCLARFVRLSAAAVAMLAAPLAARAQQFAALTPPAGYETRAP
                     IGWVDFCERNPADCQVPAMAAELVPLTAANWRTITRINTGVNRDIRAVSDKDLYGVTE
                     HWTYPSAGKGDCEDIVLEKRRRLMQAGLPRQALLITVVRDLKGDGHAVLTVRTDRGDY
                     ILDNQEAKVLNWDETGYRFIKRQSETHPNTWISLGGIDTGSSVAGMMRR"
     gene            12162..13643
                     /locus_tag="PLQ11_05240"
     CDS             12162..13643
                     /locus_tag="PLQ11_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009492252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRK24341.1"
                     /translation="MKVTVLAAAALASAAVYALPVLAQTRDSVTLCMTLEPPILDPTQ
                     GAAQAIKEVTYGNLFEGLVALTSGGKIVAKLAESWTVSPDNLTYTFKLRQGVKFHDGA
                     DLTSADVKFSFERAVAPDSKNATKKVFAPIASIETPDPLTVVLKLKEVSANLLFGLTF
                     GDASILSPASAKDAATRPVGTGAYKYVDWSRGNRLQYTRNDSWWGGKAAIRDVTYRFI
                     ADPQAQLAAIQAGDCDALTNFGASESIEILKKNPGLKVAFGNTEGETILAMNNLKPPF
                     NDLRVRRAIAHAIDRKTVIDGTMNGVATPIGTHFSPNHPAYVDLTGVTPYDPKKAKAL
                     LAEAGFPNGFSTTLKLPPPPYARRSGEIVAAMLGEVGIKVSIEPIEFPQWLERVYRNK
                     DYDLSIIAHTEPLDIEIYSRPDYYFQYNNADMQKLVKAAESTADEAARNELYRQAQKK
                     IVDDQVNVYLFMLPKTTVSKVGLNGMWENWPIPVSPAHELSWK"
BASE COUNT         2434 a         4150 c         4555 g         2590 t
ORIGIN      
        1 gcgggcgacg tggccggtcg ccagatcatt gtccatcagc gcgcggaagc ggttgaccga
       61 gccgctgcac ccggcccctt ccggcaggcg gaagccggag ggcgtgacgt tggacgagac
      121 cgtgggcgtg ccgctcgacg atgtgccggc cggtgcggag gcagcaggcg gcgcagaggt
      181 ctgattgcag gcaccgagtg ccagcggcac cagcaacacc aggacaacac ggggcagggg
      241 cgaaaccatc ggcagtctcc acgggctctt tcaacaggag agtgccggga ttcggctttt
      301 ccagcaatcg gtcatgcgtt tcgtgctgga ccatgcctgc cggagcctcc tacagtcagc
      361 gattgcgcca gcgcgtgggg gagacggcat gctgacaatc tatggtgttc acaattcgcg
      421 tgccgcgcgg gtgatctggc tggcctatga actgaacatc acctttcgac aggtgccgat
      481 catccaggcc tacaagctgg caggccgcgc gcccggtccg gaccctgtca acacccggtc
      541 gccggagttt ctgcgcgtca acccggcggg tctgatcccg gcgatcgacg atgatgggct
      601 ggtgctggct gaatcgctgg ccattaccct ctatctcgcc cgcaagcatg gcgggccggt
      661 cgcgccgcag gatatcggcg aggaggggca ggcgatgatg tggtcgttct gggcggcgac
      721 cgagatcgaa ggccatgcgc tcgacatcat gcgtaatcgt gtgatgcgtc cgccggaaga
      781 gcgcgacggc attcttgccg atgccgcaga agccgttctg aacgcgaagt ttcctgtgct
      841 ggagcgggcg ctggccagga caggctggct ggtcggcagc cggttcaccg tggctgatct
      901 caacgtggcc gaggtggtgc gctatgcgag cgcggcgccg gcgctgttcc gcacccatcc
      961 caacatcaag aaatggcttg atgcctgcca tcagcgcccg gccttccagc gcatgtggac
     1021 cgagcgggcc aacgaaaagg tctgaaagac ccggcggtga ctctgcggcg tgggcttgca
     1081 ataggccggc aaagcaggtt aaacctgtca caatcgagca ggagacgggc ccgtgacggc
     1141 tgcgaccatg accagtgcca tcgaaaggac gggcgagatc gcgccgtcgc tggcacttgg
     1201 gctgctgctg cttatccgcc cctgagggcc gtccgggcgt gtgcctgggc cttcagggga
     1261 tgcgagccaa atcccggaca atttcatttt ttgcgggcct gagcgtcgga ttggcgcagc
     1321 ccgaagccca aggatcaccg acatgacggc ttccaccaac gatcgcgtgc tgattttcga
     1381 cacgaccttg cgcgatggcg agcaatgccc cggcgctacc atgaccttcg aggaaaagct
     1441 ggaggtggcc gatctgctcg atgaactcgg cgttgatatc atcgaggcgg gctttccgat
     1501 cgcctccgat ggtgatttcg aggcggtgac ggctgttgcc aagcgggtga agaacgccac
     1561 cgtggccggc ctcgcccgtg ccggcgccaa ggatatcgac cgtgccggcg aggcggtgaa
     1621 gcacgcccgg cgcgggcgca tccacacctt catctccacc tcgcctgtgc atatgaaatg
     1681 gaagctccag aaggagccgg acgaggtgtt gcagctggtc atcaactcgg tgacccgtgc
     1741 gcgcagtctc gtcgaggatg tcgaatggtc ggccgaggac ggcacccgca ccgagatgga
     1801 ttttctctgc cgttgcgttg aagcggcgat caaggcgggt gccaccacca tcaacatccc
     1861 cgatacggtc ggctacacga cgccggaaga atatatgact ctgttccaga ctgtccggaa
     1921 ccgcgtgccc aactcggaca aggcgatctt ctcggtgcat tgtcataacg atctcggcat
     1981 ggccgtggcc aacagcctcg ccggcgtcaa gggcggcgcg cggcagatcg aatgcaccat
     2041 caacggcatc ggcgagcggg cgggcaatgc cgcactcgag gagatcgtca tggcgatcaa
     2101 gacgcgcggc gatgtgctgc cgtatcattc gcggatcgag acgacgatgc tgaaccgcgc
     2161 ctccaagctg gtcgctgccg ccacctcctt cccggtgcaa tacaacaagg ccattgtcgg
     2221 ccggaacgcc ttcgcgcacg agagcggaat ccatcaggat ggcatgctca agcataccca
     2281 gacctatgaa atcatgacgc cggaatcggt cggcgtgtcc aagacctcgc tggtgatggg
     2341 caagcattcg ggccgcgcgg cgttcaagga caagctcaag cagctcggct acgaactggg
     2401 cgagaaccag ctcgaagatg ccttcgtccg cttcaaggcg ctgtcggatc gcaagaagca
     2461 catctacgac gaggatatcg aggcgctggt cgatgagaat atcgccaccg ctcaggaccg
     2521 ggtgaaagtc ctgtcgctgc tggtcgtcgc cggcacgatg gggccgcagc tcgccacgct
     2581 gacgctcgac atcgacggcc agcacaggac cgtgcaatcc tccggcaatg gtccggtgga
     2641 tgcgacgttc aacgccatcc atgcgctgct gccgcacgaa gccaagctcg cgctctacca
     2701 ggtgcatgcg gtgacggaag gcaccgatgc gcaggcggaa gtgtcggtgc ggctggagga
     2761 caagaagtcg gggcggctgg taaccggcaa atcggccgat ccggacacgc tggtcgcttc
     2821 cgccaaggcc tatatcgttg cgctcaacaa gctgaccaac gagaagagcc agggcttcca
     2881 tgcgcagcat gtgagtttgt aaggccatgg ctaggcggcc tcagggatcg ttttcggccc
     2941 ttgtcgaggt tgatgatcaa gcgttcgatg gtatcggcat gttcgatgcc ttcggccttg
     3001 attttcgtgc ggatatagtt cctcgcgttg atgagtgggt gcagggcgcc atcattcacc
     3061 ccggcttcgg acatccgggc gatctgggtt cgcccgaacc tccggccggt gagccaacgg
     3121 tcatgcttct ggcaaaccag aatacgtttc gatgcggggt cgttctgggc gtctccgtcg
     3181 cggacgaaca ggctatgctc tcgagtgcgt ccatcttctt tgaaggcggg cgtcagcatc
     3241 agctcgcctt gcgacgcata ttggtctcca aagaccgcac gatggggctt cttgacggcg
     3301 atcttgatga cgccgtcgcc atcaccctgt ttgagccgac gttcgcgagc gagcgccaat
     3361 ggcatggaga ggggagcgtg cacgaggtgc tggtctacgg gatttgctac aggctgtcgg
     3421 tgggggctcc gccgcccatg caggtcgcgg acccgctcga tggtcgctcc ggcaaaactc
     3481 aaaccctgta ttttgatgat gcagccatgc ttattccgcg cgaacatccg ccctggctgt
     3541 ataccattat cgggcctgtg cttcaggtcg acgattacga aatgaccgtg ctcgatcagc
     3601 acgtcctgaa ggttcgtgtt gttgtcgccc gacttgatga tgacaaggat gtcgatgttg
     3661 atctgttcgt tacaaagaca gcgctgcgcg gcgggaagag gcccaaagtc ggtgacatga
     3721 tcgaagcctc agttgcgctc tgcgcccggc tgtgggagcc caacgttgcc ttttcggcgg
     3781 agtgaaataa gcgctggaca ggccgcgcac ccttcgctat agacccgcca acgacggggc
     3841 gcggccaacg cgccccggtt tcgttcgggt gattagctca gttggtagag cagctgactc
     3901 ttaatcagcg ggtcgtaggt tcgagcccta catcacccac cataacctcg cttcagtcgg
     3961 gatttcagag gcagccgtcc ttcgggcaca ggcgcgaccc gcaggcatgg ggcgcagccc
     4021 ctttagtctt ttgacggctt tgctctggat cagcatggca aggctggact tgcgatagaa
     4081 ttgcgctccg tcgtgagagg agcgcagaaa atgaaacgcg ccagcaaacg caagattgcc
     4141 gacagtgatg cggatgccag ggccggcggc gagccgggcg acacgctgga ggcagcgcgc
     4201 gccaaggccg atctttcctg ggatctcgcc gtgccgctgg tcacccatcc gctgatgctg
     4261 atcaacatcg tcaaggcgtt cttcttcagc gggctgatca tggcgctgct gctgtcgttc
     4321 ctgatcggga tcaccggcag cccggagcag attccgatga tcatcggcat cagtttcgct
     4381 gctgcgggtg gcatcggcat cgtcgggacg gcggcggccg cgctgatcta tcgcaaccgc
     4441 atgaatatgc atttcctgct gaatgaccgc ggtgcacggg cgatcattac cgatacgacg
     4501 gcgcgcaatg tgtcgatcct gaccatcatt cttggcgtcc ttgccggcaa gccgggtgcc
     4561 gtcggcaccg gactgatcac attgtcggac cgggaccggc atgccggctg gcgcggcgtc
     4621 gtcaacgttc gctatcatcc gaagcaccac gccatcagcc tcgccaattc ctggcgcacc
     4681 gtgctgatcc tgttctgcac gccagagaat tacgagaaag tcgcggccta tgtggctgca
     4741 gcgacggcgc gggccaaagt gcccgcctct gcgaagatca acccgatccc caagctgctg
     4801 atgcataccg tcggcgtcgt gctggcctgc gtgccgatgt tcgtgttcga ctatccgttc
     4861 cgcatcgaca tcttcgcgcc gctgctcatc ctgtgcttcg cgctggcgat gctgtggctg
     4921 atcccgatca tggcctatgt ggtctgggcg ggcctggcct atgtcgtgct gcacacgatc
     4981 atgaagctga tggaaacccg cacctcgcaa ttcggcggcg ggctttaccg ggcgttcgag
     5041 gtgctgagcg gcgacgatat cgcgctgctg gtcgcaacag gaatcggcat ggcctatctg
     5101 atctggctgt cgcgcgggct gatgagcggg cgggttcgct cggcgctcgg cggtgacatg
     5161 gaggaactgg acggctgatc ccggcgcctg ccaacacgaa gaaccctccc tctgcggggc
     5221 agagggaggg ctttccggct ctgtggacgc ggatcttacc ggtcgcgctg gtagttgttg
     5281 cggcagttct ggatggcggc ctgattgccg cgggcgttgg ccatgcagcg gcgataggcg
     5341 ttctcggcgt tctgccgctg gttgcattgc gccatgcagg cgttgtagcg gcgggtcagc
     5401 gcgccagagt tgacgttgtt ggagcggtca agaatgccct cgcaacgcga ccggcaggct
     5461 gcggccggcg aatattgcgc ggcagccggc aggcttgcca gtaccgtcat gccggcggcg
     5521 gcgatcagca tcagtgtcct gaacttcatg gagacctcct cgtggttcaa tgaggagagt
     5581 ctaatattat gggatttcct cgccatgatg cagcgcaacg ttgccggcac gatttcagtg
     5641 acaacaaaag atttatatcg tttctgacag aaacagtgcc tcaccctgcg gcgtagaggt
     5701 ccttgtagct gtcgcgtaga acatttttct gcaccttgcc catcgtattg cgcggcaggt
     5761 cgtcaacgaa gatcacccgc ttgggctgtt tgaatttcgc cagcctttcg gcgagatggg
     5821 cgagaacacc ggcttcggtc agcggggcgc cggccttcct gacaacaacc gcggtgacgc
     5881 cttcgccgaa atccctgtgc ggaacgccga tcaccgcgct ttcgatgacg ccggggatgg
     5941 aatcaatctc ggtctcgacc tccttggggt agacgttgaa gccgccggtg atgacgaggt
     6001 ccttgccgcg tccgacaatc tgcacatagc cgcgctcgtc gatcaggccg aggtcgccgg
     6061 tgatgaagaa cccgtccttg cggaactcgg cctcggtctt ttccggcatg cgccagtaac
     6121 ccttgaacac gttcggtccc ttgacctcca gcacgccgat gtcgccgggc cgggtcagga
     6181 tctctccggt ttccgggttg gccacccgca ccgacacgcc gggcaggggc aagccaacgg
     6241 tgccggcgat gcggtcgccg tcatagggat tggaggtgtt catgttggtt tcggtcatgc
     6301 cgtagcgttc gagaatggcg tggccggtgc gttcctgcca ttcgcggtgc gtttcggcca
     6361 gcagcggcgc cgagccggag acgaacaggc gcatgttctt cgttgcttcc cgagtcaacc
     6421 cgggatgctg gagcaggcgg gtgtagaagg tcggcacgcc catcatcgcg gttgatgtcg
     6481 ccatcagctt cagcatggtg tcggggtcga gcctgggcag gaaatgcatg gaagcgccgg
     6541 ccagcagcac aacgttggtc gccacgaaca atccatgcgt gtggtagatc ggcagggcgt
     6601 ggatcagcac atccttgttg gtgaagcgcc agtaatcgac cagcgtcagc gcgttcgagg
     6661 cgagattgtc gtggctgagc atcgcgccct tggagcgccc ggtggtgccg gaggtataga
     6721 ggatcgcgcc gagatcgtcg gggccacgga cgacatttgc aaaggaaggg gcagcggccc
     6781 tggcggcctc ggccagcgtg ccgtcgttgg cgacgcccag cgtctccagc cgcgcgccgg
     6841 ccttggcggc aaccggcgcc agcgctgcgg tcttgcccgg atccgagacg aaaaccgccg
     6901 gctcagcgtc ggtgaggaaa tactcgacct cggcggcggt ataggcggtg ttcagcggca
     6961 ggaacacagc gccggcgcgc acgcaggcga ggtagaccat caccgcttcg atcgactttt
     7021 ccacctgaac cgccacccgg tcgcccggct tcacggcgag gccggcgagc acattggcat
     7081 agcgcgccga aaggtcgagc atgtcgccat agctgtagct ccggccgtca ggcagcatga
     7141 caaaggcggc gtccttcgcc ttgatccgcg caacgatggt gtcgaacagg tggttggcca
     7201 tgatgtcgct ccggcaggtc attgccccgg gggttttgcg ctattgagac agggggcgca
     7261 agggggctgt caggcgtggg gcagctgtcg gatgccagga tgcagattgg gcttgcacag
     7321 gcggcgggaa tggcgatgca ttgccgatcg ggaggctgca tcgcctcctc accaagacga
     7381 ccggggccaa gcgcaatgcc aggacatgat cttctgcttc ctcctgccgc caccattgcc
     7441 cgcgtttttg cggagatcga tgcgcggcgc gatgcggtgg cagagctgac cgccgaactg
     7501 atccgcttta cgacggtcaa tccgccgggg cgcgactacg agccttgcgc cgagttcatc
     7561 gccgaacggc tggcgaattc gggcttttcc gccgagttcg tgcgcgggcg gggcgaagcg
     7621 ggcgacagcc acgactttcc gcgcgccaat gtcatctgcc gacgcgaggg gacgggcgag
     7681 gggccctgcg tgcatttcaa cagccacatt gatgtggtcg aggcggggca tggctggacg
     7741 gtcgagccgt tcgacggggt gatccgcgac gggcgtgtct acgggcgcgg tgcctgcgac
     7801 atgaagggcg ggctggcggc ctcgatcatc gctgtggagg cgatagtggc ggcgggcgtg
     7861 ccctatcgcg gggcactgga aatctccggc acggcggatg aggaatcggg cggcttcggc
     7921 ggtgtcggct atctggcaaa gcggggctat ttctcgccgc cgcgcgtcga tcatgtcatc
     7981 atccccgagc cgctcaatcc ggaccgggtc tgcctcggcc atcgcggcgt gtggtgggcg
     8041 gaagtggaga tgcgcgggcg gatcggccat gggtcgatgc cctttctggg cgtgtccgcg
     8101 atccgcggca tggccgattt catggcgcgg atagaggccg atctctaccc catgctcgac
     8161 gggcgccgca cggcgatgcc ctgcgtgccg ccgggcgcgc gtgcctcgac cctcaactac
     8221 aattcgatcc atggcggcca acccgagccc gaggacgggt tgccggcacc ggtggtggcc
     8281 gactggtgcc ggctggtgct cgaccgtcgc tatctgatcg aggagcggct cgaccaggta
     8341 aaggcggaga tcatcggcct gctcgatgcc accaaggcgg cgcgtgccgg gctcgactat
     8401 tccatccgcg aattgatgca tttcgcgccg gtgatgaccg aggccggcga tccggtggtc
     8461 aaggcgctcg atcactggat cgaaacgctg ttcgaccgcc cggcagcgca tgtcgcttca
     8521 cccggcacct atgaccagaa gcacatccag cgtttcggcg ccctcagaag ctgcgttgcc
     8581 tacgggccgg gcattctcga tctggcgcat cagccggatg aatgggtcgg catcgacgac
     8641 atggtgcgct cggccaaggt gatggcggcg gcagcactga cgctgacggg gatcagccct
     8701 ggttagaaag tcggcaattg aacttgcggc gggcctgctt tggtgtgaag ctttgtcatg
     8761 gacgaaacgg acctcctgcg gcgccttggc gttgctctgg caatcggcct tctggtcggg
     8821 ctggagcgcg gctggcacac gcgggaagaa cccgattcca tgcgcccggc gggcctgcgc
     8881 accttcgcgc tcaccggatt gcttggcggc attgccgggg cgctttcgct gaagctgggc
     8941 ggtgtggtgc tggcggcttc cttcgccacg ctttcggccg gcttcatcgc tttccactgg
     9001 ctggaagcgc agaaggaggg cgatctcggc gcaacgaccg tggttgccgg catgctgacc
     9061 ttcctgctcg gttcctatgc gctggtcggt gaagtcacca atgcggtgat cgccggagtt
     9121 tcgatgacca tcctcttggc ctcccgccag cggctgcata cgatggttgc ctcgctgaca
     9181 ccggaggaaa tccgcgccat cctgaccctg gtggcgatgt cgttcctgct gctgccgctg
     9241 ctgccgaacc ggccggtcga cccatgggga gcgatcaatc cctatctgat ctggctgatg
     9301 gcgatcacca tcgcgctggt gtcgttcggc ggttatgtcg cggtcaaggt gttcgggcag
     9361 gaacgtggcg tgctgctgac ggccgtggcg ggcgggcttg cctcatcgac ggcggtcact
     9421 ctgtcctttg cgcggctggc acgcgagcat ccgcagtcgg agcggctgct ggcgtcgggc
     9481 atcctggcgt cgggcgcgac gatgatggtg cgcgttgcgg tgctggccgg cgtgctcaat
     9541 gccaaactga tcacgccgct tgcgctgccg ctgggtgcgg cggcgctggt gatgctgttc
     9601 ggcggctggc tgctgctcgg tggcgcccgc gatgacgagc agcccaggct tgatgtcacc
     9661 aacccgctgg aactgggcac cgcgctgaag ctggcggggc tgatcctcat catcatgctg
     9721 gccggcaagt tgatgaaggc gcagtttggc gatgccggcg tgatggcact ggcggcggtc
     9781 tcgggtatcg ccgatgtcga tgcgatcacg ctgtcgatgg cccagtcggg gggaaaagac
     9841 ctgaccttgg cggttgcagc ggcggccatc ctgctggcgg tggcggtcaa cagcctggcc
     9901 aaggtcgggc tggccggctg ggaaggaggc acccggctgg gtttcaagac cggaactgtc
     9961 agtttactgg ccattgcagt ggcgggggcg gttgcctatt ttctctgatg cccgggcagg
    10021 gagcgcgcgg gcagcaaaaa gaccatcaga gggaggatct tttccatgcg cattgtcgca
    10081 acggcattcg cactggccct ggggttaagc ccggccatcg cccagcctta tccaaacaag
    10141 ccgatctcgc tcgtcgtgcc gttcgcagcc ggcggtccca cggatctgat cgcccggctg
    10201 gtcggagaat cgatgaccaa gacgctcggt cagcagatca tcgtcgagaa catcgccggc
    10261 gccggcggca cgatcggccc gtcccgcgtg gcccgggcca agccggatgg ctatactttt
    10321 cttgtccacc atgtcgggct gacgacggcg gcgacgctgt atcgcaagct tgattttgat
    10381 atccgcacgg cgtttcagcc tgtgggcatc atcaccaacg ggccgatgat cctgattgcc
    10441 aaatcggatt tcgagcccaa ggattcggct gaactggtcg ccttcctcag gaagaacggc
    10501 gaaaaggtga cgtttgccac tgccggcctc ggctccgggt cgaacctgtg cgggatgctg
    10561 ctggaagccg cactcaaggt caagttgacc accgtgccct atcgcggcac cggcccggtg
    10621 atgcaggatc tccgcggcaa acaggtcgat atgtcgtgcg atcaggcgac caatgcgacg
    10681 cagccggttc gcgagaattt cgtcaaggcc tatgcagtga cggcgccggc acgccttggg
    10741 ccgttaccca acattccgac cacaggtgaa aacccggatc tcaagtcgct tgacctgaat
    10801 atctggaacg ccctgttcgc gcccaagggg acgccgccgg agatcgtcag caaggttgcc
    10861 gaggcgctgc ggatcgcgct gaaggaagag aaggtcgtca cccgcttcaa ggatctgagc
    10921 accgatcctg ccacccctga acaggcgacg ccggaagcgc tggccaaggc cttcacagcc
    10981 gagatcgagc gctggaagcc gctgatcgag gcaagcgggc aatatgcgga ttgacgccgg
    11041 gggccctcgg ttctgaccgt tcggtcatcc ctcgggcatt cctcgcaacc gggccttacg
    11101 gccctgctcg gcgtcgcttt cgctcctaac caacagccca ccgtcatgac ctatcggtca
    11161 tttccggtgg ccgttggtac aagcaagggc ttttttgtcc cgcaaggacg gaatgaaaag
    11221 ggggcctcgc gccccctttt ttagctcaac gccggctttc gtgcgcctca attcaccgcc
    11281 gcatcatgcc ggcaacgctg gagccggtat caatgccgcc cagtgaaatc caggtgttgg
    11341 gatgggtttc ggactgccgc ttgatgaagc ggtacccggt ctcgtcccag ttcagcacct
    11401 tggcttcctg attgtcgaga atgtaatcgc cacggtcggt gcggaccgtc aggacagcgt
    11461 ggccatcacc cttgagatcg cgcacgacag tgatcagcaa cgcctggcgg ggcaggccag
    11521 cctgcatcag gcgtcggcgc ttttccagaa cgatgtcctc gcaatcgccc ttgccggcgg
    11581 acggataggt ccagtgttcg gtcacgccgt agagatcctt gtcggaaaca gcgcggatgt
    11641 cgcggttcac gccggtattg atgcgggtga tcgtccgcca gttggcggcc gtcagcggca
    11701 ccagttccgc cgccatggcc ggtacctggc aatcggcggg attgcgctcg cagaaatcca
    11761 cccagccgat cggcgcgcgc gtctcgtatc cggccggcgg ggtcagcgcg gcgaattgtt
    11821 gcgcccgggc cgccagcggt gcggcgagca ttgcgacagc tgcagccgac aggcgaacga
    11881 agcgggcgag acaaacggcc atgcgacaag ttcccgaaaa ccaacgccgg caccttgccc
    11941 gtttacgaag gttaaggaaa ggtgaatatt aacacatcgt taacgccggc cggcagcatt
    12001 ggggattggc ttgcatcgac aacaggttga gcggcaggcc ggcggtcaga ttacgatcaa
    12061 ttgacagggc agtggggctt ttcgctgctg gcaaaggtgc atagatgtta gtgtgccgca
    12121 gcgggcggcg ttcggtcgtg tctgtttccg ggagggaaac catgaaggtg acggttcttg
    12181 cagcggctgc gcttgccagc gctgcggtct atgcgctgcc ggtgctggcg caaaccaggg
    12241 acagcgtgac gctgtgcatg acgcttgagc cgccgatcct cgatccgacg caaggggcgg
    12301 cgcaggcgat caaggaggtc acttacggca acctgttcga ggggctggtg gcgctgacga
    12361 gcggcggcaa gatcgtggcg aagctggcgg aaagctggac ggtctcgccc gacaacctga
    12421 cctacacgtt caagctcagg caaggcgtca agttccatga cggggcggat ctgacctcgg
    12481 cggatgtgaa gttctcgttc gaacgggcgg ttgctcccga ttccaagaat gcgaccaaga
    12541 aggtgtttgc cccgatcgct tcgatcgaaa cgcctgatcc gctgaccgtg gtgctgaagc
    12601 tgaaggaggt ttcggccaat ctgctgttcg gcctcacttt cggcgatgcc tccatcctgt
    12661 cgccggcctc cgccaaggat gcggcgacca ggccggtcgg cacaggcgcc tacaaatatg
    12721 tcgactggag ccgcggcaac cgcctgcaat acacccgcaa cgatagctgg tggggcggca
    12781 aggcggcgat ccgggacgtg acctaccgct tcattgccga tccgcaggcg cagcttgcgg
    12841 cgatccaggc cggcgattgc gatgcgctga ccaatttcgg cgcctccgag agcatcgaga
    12901 tcctgaagaa gaaccccggt ctgaaggtgg cgttcggcaa taccgagggc gagaccattc
    12961 tggcgatgaa caacctcaag ccgccgttca acgatctcag ggtgcgcagg gcgatcgctc
    13021 atgccatcga ccgcaagacg gtgatcgacg ggacgatgaa cggtgttgcc acgccgatcg
    13081 gcacgcattt ttcgcccaac catcccgcct atgtcgatct gaccggtgtg accccgtatg
    13141 atccgaaaaa ggccaaggcg ctgctggccg aagcaggatt tcccaacggc ttttcgacca
    13201 ctctgaagct gccgccgccg ccctatgcgc gccgctcggg cgagatcgtc gcggcgatgc
    13261 tgggtgaggt cggcatcaag gtgtcgatcg agccgatcga gtttccgcag tggctggagc
    13321 gggtctatcg caacaaggac tatgacctgt cgatcatcgc ccataccgag ccgctcgaca
    13381 tcgagatcta ctcgcggccg gactactact tccaatacaa caacgccgac atgcagaagc
    13441 tggtcaaagc ggctgaatcc accgccgacg aggcggcgcg caacgaactc taccgccagg
    13501 cgcagaagaa gatcgtcgac gatcaggtca acgtctatct gttcatgctg cccaagacga
    13561 cggtcagcaa agtcggcctc aacggcatgt gggagaactg gccgatcccg gtctcgccgg
    13621 cgcatgaact gagctggaag tgagcgggcg tccttcccct tctcggggag agttggggag
    13681 gggtggaggt gccagttcgt cccttccccc ttgcggggaa gggccgggg
//
LOCUS       DAOMXG010000052        13527 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_2506_length_13527_cov_9.269893, whole genome shotgun sequence.
ACCESSION   DAOMXG010000052 DAOMXG010000000
VERSION     DAOMXG010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 13527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13527
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2506_length_13527_cov_9.269893"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="PLQ11_05245"
     CDS             <1..579
                     /locus_tag="PLQ11_05245"
                     /inference="COORDINATES: protein motif:HMM:NF015551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HRK24342.1"
                     /translation="WPGPVLVAPQFGIEGLVNIALPLFIVTMASQNITGLTVLASFGY
                     RPDPRLGLAVTGGLSALTAPFGAPTINYAAITAALCAGPEAHPDPDRRYVAAIVSGIG
                     YILLAGLAALAADFVTRSSPVLIEAVAGLALISAFGGALLGAVQSENDRLPALLTFLV
                     TASGFTFHGVGAAFWGLLIGCAIAAFGRAGRG"
     gene            complement(620..1246)
                     /locus_tag="PLQ11_05250"
     CDS             complement(620..1246)
                     /locus_tag="PLQ11_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HRK24343.1"
                     /translation="MILIGQYDSPFVRRVGIALTLYKLAFEHRPWSAFADAFRIRPYN
                     PLTRVPTLVLDDGEALIDSAYILDYLDSLVPESVALAPRREPERRRVLKIAALATGLG
                     DKAVNLFYEQRMHNAASDIYVHRLRAQIDGALGVLEAERGKCSGSWFFGDALTHADIA
                     VGCIWSFVMAAHPGLVAAEDYPCLSALSARLEALPVFQLIHQPFIAPT"
     gene            1424..2059
                     /locus_tag="PLQ11_05255"
     CDS             1424..2059
                     /locus_tag="PLQ11_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HRK24344.1"
                     /translation="MRDPIETYMNLVPMVVEQSNRGERAFDIFSRLLRERIIFLTGPV
                     EDGMASLVVAQLLFLEAENPKKEISLYINSPGGVVTSGLSIYDTMQFIRCPVTTLCTG
                     QAASMGSLLLAAGEKDMRFALPNARIMVHQPSGGFRGQATDILIHAEEIKGLKRRLNE
                     IYVHHTGNDYETIEKALERDNFMSADKAKDFGLIDKVVSKRGEDPAEPKPM"
     gene            2297..3562
                     /gene="clpX"
                     /locus_tag="PLQ11_05260"
     CDS             2297..3562
                     /gene="clpX"
                     /locus_tag="PLQ11_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HRK24345.1"
                     /translation="MSKQSGGESRSTLYCSFCGKSQHEVRKLIAGPTVFICDECVELC
                     MDIIREESKSSLVKTRDGIPTPKEICKVLDDYVIGQFHAKRVLSVAVHNHYKRLNHAT
                     KHNDVELAKSNILLVGPTGSGKTLLAQTLARILDVPFTMADATTLTEAGYVGEDVENI
                     ILKLLQASDYNVERAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKIMEGTV
                     ASVPPQGGRKHPQQEFLQVDTTNILFICGGAFAGLEKIISGRGKGTSIGFAAKVEAPD
                     DRRTGEIFRTLEPEDLLKFGLIPEFVGRLPVIATLEDLDEATLKQILTQPKNALVKQY
                     QRLFEMENVDLTFQDEAVTMIARKAIERKTGARGLRSIMEGILLDTMFDLPGLEGVEQ
                     VVIGAEVIEGNAKPLYIYSDRDEKQAESA"
     gene            3750..4067
                     /locus_tag="PLQ11_05265"
     CDS             3750..4067
                     /locus_tag="PLQ11_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24346.1"
                     /translation="MFFAIFGAGLHFLRAAIGANERPIVESGLLYRMTDHWFAKHVLK
                     AEFTDNGAWEMLSGRNLLISMATTAIWPLFFGIFWWQRQPEALAAGCKVYGWLGLQPV
                     FCW"
     gene            4247..6673
                     /gene="lon"
                     /locus_tag="PLQ11_05270"
     CDS             4247..6673
                     /gene="lon"
                     /locus_tag="PLQ11_05270"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HRK24347.1"
                     /translation="MTAQKPKPPLIPGQVEVCPVLPLRDIVVFPHMIVPLFVGREKSI
                     KALEEVMRSDRYILLATQKNKDDDDPSTDGIYEIGTLATVLQLLKLPDSTVKVLVEGA
                     GRARIRRYVREDGHLEAEAEALLDDLGDRVEVEAMARSVVTEFEGYVKLNKKVSPDVV
                     TAVTQIEDYAKLADTVASHLAVKIADKQAILEMPKVADRLERVLGLMESEISVLQVEK
                     RIRTRVKRQMEKTQREYYLNEQIKAIQKELGDGEEGKDELGELEDRIRRTKLSKEARD
                     KSLHELKKLRQMSPMSAEATVVRNYLDWMLSLPWGKRSKVKKDLKLAQDTLDADHFGL
                     DKVKERIVEYLAVQQRANKLTGPILCLVGPPGVGKTSLGKSIARATGREFVRMSLGGV
                     RDEAEIRGHRRTYIGSMPGKIIQSLRKAKTQNPLFLLDEIDKMGMDFRGDPSSALLEV
                     LDPEQNSTFADHYLEVDYDLSNIMFVTTANSLNIPAPLMDRMEIIRIAGYTEDEKAAI
                     VRKHLIPKSLAKHGLSDKEWSISDEALLQLVRRYTRESGVRNLEREISNLVRKAVKTI
                     LLEKVASVKVTVKNLADFLGPPKYRYGEAESEDQVGVVTGLAWTEVGGELLTIEGVMM
                     PGKGKMTVTGNLKDVMKESISAAASWVRSRCLDFGVAPPLFDKRDIHVHVPEGATPKD
                     GPSAGIAMATTIVSILTGIPIRRTIAMTGEVTLRGRVLPIGGLKEKLLAALRGGLKTV
                     LIPEDNVKDLADIPSNVKNGLEIIPVSRMDEVLKHALLRQPTPIEWDEEAHEKAAGKA
                     VGETGASAPH"
     gene            7086..7162
                     /locus_tag="PLQ11_05275"
     tRNA            7086..7162
                     /locus_tag="PLQ11_05275"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7120..7122,aa:Val,seq:tac)
     gene            complement(7312..7656)
                     /locus_tag="PLQ11_05280"
     CDS             complement(7312..7656)
                     /locus_tag="PLQ11_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019906239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24348.1"
                     /translation="MKRFVLAAASVLILATAAGTASAQYYDPYRPAPPRYDPYDGPRG
                     YDPYDRPRRVDPYRYDRPPPRDWGYRPPRRQPMGNVCVTSRGACNSYLTPIGTGCRCN
                     IPGFGLKRGTIQ"
     gene            7906..8622
                     /locus_tag="PLQ11_05285"
     CDS             7906..8622
                     /locus_tag="PLQ11_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24349.1"
                     /translation="MRLSLLTAFVAYASVAALAQPARAADMTFSTLSYGSSRQCVDDC
                     PRIMQASGQITASTPQALIDFARREVARPGLINVLLIHSPGGSVNASIAMGRVLRKMG
                     TTVIVARAMDIRELPTATIGRREPGVAENLRFVSGTCASACVYALAGGLKRVVPQESR
                     IAVHRMAGNIANFDPATREFQQRQIYAGETELNALTAYIYEMGISTDLVDMAESVPHE
                     NARILSNREIRALRLGQPKL"
     gene            8722..8798
                     /locus_tag="PLQ11_05290"
     tRNA            8722..8798
                     /locus_tag="PLQ11_05290"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8756..8758,aa:Asp,seq:gtc)
     gene            8975..9340
                     /locus_tag="PLQ11_05295"
     CDS             8975..9340
                     /locus_tag="PLQ11_05295"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007531776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HRK24350.1"
                     /translation="MQLLLADYFPLVVFIGLSAVIGLALLAAPFLLAFKAPDAEKLSA
                     YECGFNAFDDARMKFDVRFYLVAILFIIFDLEVAFLFPWAVAAQEIGWFGFWSMMLFL
                     GVLTIGFIYEWNKGALEWD"
     gene            9386..9967
                     /locus_tag="PLQ11_05300"
     CDS             9386..9967
                     /locus_tag="PLQ11_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HRK24351.1"
                     /translation="MVSAVDRGNPLVAPAPRGLVGADGRPIGSTDPYFATINNELADK
                     GFIVTATDELIAWARTGSLMWMTFGLACCAVEMMQASMPRYDVERFGFAPRASPRQSD
                     VMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVPV
                     DIYVPGCPPSAEALLYGILLLQKKIRRTATIER"
     gene            9973..10206
                     /locus_tag="PLQ11_05305"
     CDS             9973..10206
                     /locus_tag="PLQ11_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24352.1"
                     /translation="MAAEPKDMIVPMLKEMRAENAALHGDTRKRLDKIEQAKKGFRNA
                     LAADSLMSKMMIGDWEERIEALEKKVRKLESSK"
     gene            10203..10805
                     /locus_tag="PLQ11_05310"
     CDS             10203..10805
                     /locus_tag="PLQ11_05310"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014130488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HRK24353.1"
                     /translation="MTDKLQALGEHIAASLPGSVTGFDVAFGDLNVTARAADIVKVMT
                     FLQGDARCLFWSFIDVCGVDYPGRDNRFDVVYHLLSPRHVQRVRVKVETDEATPVPSI
                     TGLFPGADWFEREAYDMYGILFSDHPDLRRLLTDYGFEGHPLRKDFPTTGYVEVRYDD
                     QEKRVVYEKVKLNQEMRQFDFLSPWEGTDYVLPGDEKAKQ"
     gene            10857..11222
                     /locus_tag="PLQ11_05315"
     CDS             10857..11222
                     /locus_tag="PLQ11_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012460472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRK24354.1"
                     /translation="MAIQSHRDLRVWNAAVELSVACYRLTADFPREEAYGLTSQIRRA
                     SVSIAANIAEGYGRDNVGSYIQFLRIAQGSLKELETLLLLAERIPLCDSRQIVPLTQQ
                     SDGIGRMLRSLIRTLQDPA"
     gene            11281..12483
                     /locus_tag="PLQ11_05320"
     CDS             11281..12483
                     /locus_tag="PLQ11_05320"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HRK24355.1"
                     /translation="MTTAPDTPAHLRNFQINWGPQHPAAHGVLRLVMELDGEIVERCD
                     PHIGLLHRGTEKLIEQKTYLQAVPYFDRLDYCAPMNQEHAFCLAIERLLGVEVPRRGQ
                     LIRVLFSEIGRILSHLLNITTQAMDVGALTPPLWGFEEREKLMVFYERACGARLHANY
                     FRPGGVHQDLPEDLINDIEAWCDPFLKVVQDLDDLLSENRIFKQRNVDIGYVSIEDAW
                     KRGFSGVMVRGSGAAWDLRRATPYECYSEMEFDIPIGKNGDCYDRYHIRMEEMRQSVR
                     IMRQCIVKMRQSDGKGPVMAGGKVSPPKRGEMKRSMEALIHHFKLYTEGFKVPAGEVY
                     AAIEAPKGEFGVYLVADGTNRAYRCKIRAPGYAHLQAMDFMTKGHMLADVSAVMGSFD
                     IVFGEVDR"
     gene            12508..12780
                     /locus_tag="PLQ11_05325"
     CDS             12508..12780
                     /locus_tag="PLQ11_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24356.1"
                     /translation="MFAAGGTTAALLAGALQAQAEVPSPRGPAPPIVTLEQLAREGYE
                     VKAIQSAATRGFGFVVMLQRGGEVKSCLMRIERNADGRPARQSVCF"
     gene            12793..>13527
                     /gene="nuoE"
                     /locus_tag="PLQ11_05330"
     CDS             12793..>13527
                     /gene="nuoE"
                     /locus_tag="PLQ11_05330"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HRK24357.1"
                     /translation="MSVRRLAPDAIQPASFTFSAENRAWIVTQLEKYPAGRQASAVIP
                     LLWQAQKQAGGWLPRAAIEHVGDLLGMPYIRVLEVATFYTMFNLEPVGKYFIQLCGTT
                     PCALRGAEKIIKVCKDRIGEQRHVTADGMFSWLEVECLGACCNAPMVQINDDFYEDLT
                     PENFNKLLDDLAAGRKVKVGSQSGRVSSEPEGAVKTLLDAGLYDGSAVGNWRKGFDER
                     YSKAQAAKAAAAAATAPAAPPPAAKPV"
BASE COUNT         2504 a         4098 c         4381 g         2544 t
ORIGIN      
        1 tggcccggcc cggtgctggt ggcgccgcag ttcggtatcg aagggctggt caatatcgcc
       61 ctgccgctgt tcattgtcac catggccagc cagaacatca ccggccttac ggtgctggcg
      121 agctttggct accggcccga tccgcgtctt ggcctggcgg tcaccggcgg tctttcggcg
      181 ctgacagcac cgttcggcgc gccgaccatc aactatgcgg cgatcaccgc cgcgctctgt
      241 gccggaccgg aagcccatcc cgatcctgac cgccgctatg tcgcggccat tgtctccggc
      301 atcggctaca tcctgctggc ggggctggcg gcgctggcgg ccgacttcgt cacccgctcc
      361 tcgcccgttc tgatcgaggc agtggcgggg ctggcgctga tctcggcctt tggcggcgcg
      421 ctgctcggcg ccgtgcagtc cgagaacgat cgcctgccgg cgctgctgac ctttcttgtc
      481 acggcttcag gctttacatt tcacggtgtc ggggcggctt tctgggggct gctgatcggt
      541 tgcgcgatcg ctgccttcgg gcgggccggc cgcggctgat caaaaaattt gcagcgtccc
      601 gccactcttg gcgccgccat cacgtcgggg cgatgaacgg ctggtggatc agttggaaca
      661 ccggcagcgc ttcaagtctt gccgacaggg ccgacaggca ggggtaatcc tcggccgcca
      721 ccaatcccgg atgggctgcc attacgaacg accagatgca gccgacggca atgtcggcat
      781 gggtgagcgc atcgccgaag aaccacgagc cgctgcactt gccgcgctcg gcttccagca
      841 caccgagagc cccgtcaatc tgcgccctga gccggtgaac atagatgtcc gaggccgcat
      901 tgtgcatgcg ctgctcgtag aacaggttca ccgccttgtc gccgagaccg gtcgccagcg
      961 cggcgatctt cagcacacgc cggcgctccg gttcgcggcg cggcgccagc gccacgctct
     1021 cgggcaccag actgtcgaga tagtcgagga tataggcgct gtcgatcagc gcctcgccat
     1081 cgtcgagaac cagcgtcggc acccgggtca gcggattgta ggggcggatg cggaaggcat
     1141 cggcaaaagc cgaccacggc cggtgttcaa acgcgagctt gtagagggtg agggcaatgc
     1201 caacgcggcg gacgaagggc gaatcgtatt gaccgatcag gatcatcagg aactctggga
     1261 ttgatgggga tggcgcagcc ttgccgccaa ctatagccta atcgtggcac aggccaccct
     1321 ttccgattca cgcctcggct actattttcg tgcaacgagt ggacgtaaca gcgattcgag
     1381 acgcggggaa acggtccccg cgctgcaccc ggcgagaggc accatgcgcg atcccatcga
     1441 aacctacatg aacctcgtgc ccatggtggt cgagcaatcg aaccgcggcg agcgcgcctt
     1501 cgacatcttc tcgcgcctcc tgcgtgagcg catcatcttc ctgaccggtc cggtcgagga
     1561 tggcatggcc tcgctggtcg tcgcccagct gctgtttctc gaagcggaaa accccaagaa
     1621 ggaaatctcg ctgtatatca actcgccggg cggcgtggtc acctccggcc tgtcgatcta
     1681 cgacacgatg cagttcatcc gctgcccggt caccactctg tgcacggggc aggcggcctc
     1741 gatgggctcg ctgctgctgg cggccggcga gaaggacatg cgctttgccc tgccgaacgc
     1801 ccgcatcatg gtgcatcagc catccggcgg cttccgtggc caggcgaccg atatcctgat
     1861 ccacgccgaa gagatcaagg ggctcaagcg gcgcctcaac gagatctacg tgcaccacac
     1921 cggcaacgat tacgaaacga tcgaaaaggc tctggagcgg gacaatttca tgagcgccga
     1981 caaggccaag gacttcggcc tgatcgacaa ggtggtctcc aagcgcggcg aggacccggc
     2041 tgaacccaag ccgatgtgat cggcgggtag tcgtcaggtg gttttgtgcc tataaggggg
     2101 ctctgcgccg ccgatcggta acggcccgtt agggaaacgg ggcggggatc attccggcgc
     2161 ggattgcatt aacggttcct ccatcgaacc gatgggacaa tggaacctga gtgaatcggt
     2221 atggtccgca gaaccattca gggatgcggc acgcgtggcg ggaagcgacg ttttcttcct
     2281 cgccggggag aaggtgatga gcaagcagtc cggtggagaa tcgcgcagca cgctgtactg
     2341 ctcgttctgc ggaaagagcc agcatgaggt gcgcaagctc atcgccggtc cgactgtgtt
     2401 catctgtgat gaatgcgtcg aattgtgcat ggatatcatc cgcgaggagt cgaaatcctc
     2461 gctggtcaag acccgtgacg gcatcccgac gccgaaggaa atctgcaagg tgctggacga
     2521 ttatgtgatc ggccagttcc acgccaagcg cgtgctgtcc gtggcggtgc acaaccacta
     2581 caagcgcctc aatcacgcca ccaagcacaa tgatgtcgaa ttggccaagt cgaacatcct
     2641 gctggtcggg ccgaccggtt cgggcaagac gctgctggcc cagacgctgg cgcgcatcct
     2701 cgatgtgccg ttcaccatgg ccgacgccac caccctgacc gaggccggct atgtcggcga
     2761 ggacgtcgag aacatcatcc tcaagctgct ccaggcctcc gactacaacg tcgaacgggc
     2821 ccagcgcggc atcgtctata tcgacgagat cgacaagatt tcccgcaagt ccgacaatcc
     2881 gtcgatcacc cgcgacgtgt cgggcgaggg tgtgcagcag gcactgctga agatcatgga
     2941 aggcacggtg gcatcggttc ccccgcaggg tggccgcaag catccgcagc aggaattcct
     3001 gcaggtagac accaccaata tcctgttcat ctgcggcggc gccttcgccg ggctggaaaa
     3061 gatcatttcc ggtcggggaa agggcacgtc gatcggcttt gccgccaagg tcgaggcgcc
     3121 ggacgaccgg cgcaccggcg agattttccg cacgctggag ccggaggatc tgctcaagtt
     3181 cggcctcatc ccggaattcg tcggtcgtct gccggtgatc gccacgctcg aggatctcga
     3241 tgaggcgacg ctcaagcaga tcctgacgca gccgaagaac gcgctggtca agcagtatca
     3301 gcggctgttc gagatggaga atgtcgacct gaccttccag gacgaggcgg tgacgatgat
     3361 cgcccgcaag gccatcgagc gcaagaccgg tgcgcgcggc ctcagatcca tcatggaagg
     3421 catcctgctc gatacgatgt tcgatctgcc gggccttgag ggcgtcgagc aggtggtcat
     3481 cggcgccgag gtgatcgagg gcaacgccaa gccgctctac atttactcgg accgcgacga
     3541 aaagcaggcg gagtcggcct gattgtcctg cccgatggtg ttgatcaatt agcgcatttt
     3601 caagcgaagt ggagtccggt tcgcgtgaag aaaatgcgac aagacaagat gttagggcat
     3661 tttcagtgaa tgtgaattca ctgaaagtgc tctagcggaa cggcatcgta gcgatggggc
     3721 gatatcttct cttggtcggg atgacgacag tgtttttcgc catctttggc gccggtctgc
     3781 attttctgcg cgcggcgatc ggtgccaatg aacgcccgat cgttgagagc gggcttctct
     3841 acaggatgac cgatcattgg tttgccaagc atgtgttgaa ggcggagttt accgacaacg
     3901 gcgcctggga aatgctgagc ggtcgcaacc tgttgatttc catggcaacg acggcgatct
     3961 ggccgctgtt tttcggcatt ttctggtggc agcgccagcc tgaggcgctg gctgccggtt
     4021 gcaaggtcta cggctggctc gggttgcagc cggttttttg ttggtgagcg cctttccggc
     4081 gcacgttccc ttgaatcatc gacaaattcg ccccacttta tcggtgaagg tagcgggagt
     4141 gccgctgagt cgcgggctcc gccggcttga cgcgtaagat cggtcaggcc gggccgttgc
     4201 catcgggagc cgctgttttc cgcgtgcggc gtcgaaagga caccagatga ctgcccagaa
     4261 gcccaagccc ccgttgatcc ccggacaggt cgaggtctgc cccgttctgc cgctgcgcga
     4321 catcgtggtg tttccgcaca tgatcgtgcc gctgtttgtc ggccgcgaga agtcgatcaa
     4381 ggccctggaa gaggtgatgc ggtcggatcg ctacattctt ctggcgaccc agaagaacaa
     4441 ggacgacgat gatccgtcca ctgacggcat ctacgagatc ggcacgctgg cgacggtgct
     4501 gcaactgctc aagctgccgg acagcacggt caaggtgctg gtggaagggg ccgggcgtgc
     4561 ccgcatccgc cgctatgtgc gcgaggacgg gcaccttgag gctgaagctg aagcgctgct
     4621 ggatgatctc ggcgaccggg tcgaggtgga agcgatggcc cgctcggtgg tgaccgagtt
     4681 cgaaggctat gtgaagctca acaagaaggt ctcgccggat gtcgtcaccg cggtgacgca
     4741 gatcgaggat tacgccaagc tggccgatac ggtcgcctcg catctggcgg tcaagattgc
     4801 cgacaagcag gcgattctcg aaatgccgaa ggtggccgac cggcttgagc gcgtactcgg
     4861 gctcatggaa agcgagattt ccgttctcca ggtggaaaag cgcatccgca cccgcgtcaa
     4921 gcggcagatg gaaaagaccc agcgcgaata ctacctcaac gaacagatca aggcgatcca
     4981 gaaggaactt ggcgacggcg aggaaggcaa ggacgaactc ggcgaactcg aggacaggat
     5041 ccgccgcacc aaactatcga aggaggcgcg cgacaagtcg ctgcacgagc tcaagaagct
     5101 ccggcagatg tcgccgatgt cggccgaggc gacggtggtg cgcaactatc tcgactggat
     5161 gctgtcgctg ccctggggca agcgctcgaa ggtcaagaag gacctcaaac tggcgcagga
     5221 tacgctcgat gccgatcatt tcggcctcga caaggtcaag gagcgcattg tcgaatatct
     5281 ggcggtgcag cagcgcgcca acaagctgac cgggccgatc ctctgccttg tcggcccgcc
     5341 cggtgtcggc aagacctcgc tcggcaagtc gattgcccgg gcgacgggcc gcgaattcgt
     5401 ccgcatgagc ctcggcggcg tgcgtgacga ggccgagatc cgcggccacc gccgcaccta
     5461 catcggttcg atgcccggca agatcatcca gtcgctgcgc aaggcgaaaa cgcagaatcc
     5521 gctgttcctg ctcgacgaga tcgacaagat gggcatggat ttccgcggcg atccgtcctc
     5581 ggcgctcctg gaggtgctcg accccgagca gaactcgacc ttcgccgacc attatctgga
     5641 ggtcgattac gatctttcga acatcatgtt cgtcaccacg gccaattcgc tgaacattcc
     5701 ggcgccgctg atggatcgca tggagatcat ccgcatcgcc ggctataccg aggatgagaa
     5761 agcggcgatc gtccgcaagc acctgatccc caagtcgctc gccaagcacg ggctgagcga
     5821 caaggaatgg tcgatctcgg acgaggcgct gttgcagctc gtccgccgct acacgcggga
     5881 atcaggcgtt cgcaaccttg agcgcgagat ttcgaatctg gtccgcaagg cggtcaagac
     5941 gatccttctg gaaaaggtcg cctcggtgaa ggtgacggtg aagaacctcg ccgatttcct
     6001 cgggccgccg aaatatcgct atggcgaggc cgagagcgag gatcaggtcg gtgtcgttac
     6061 cggtctggcc tggaccgagg tgggtggcga actgctgacc atcgaaggcg tcatgatgcc
     6121 cggcaagggc aagatgacgg tgacgggcaa cctcaaggac gtgatgaagg aatcgatctc
     6181 cgccgccgca tcctgggtgc gctcgcgctg cctcgatttc ggcgtcgcgc cccccctgtt
     6241 cgacaagcgg gacatccatg tgcatgtgcc ggaaggcgcc acgcccaagg acggcccctc
     6301 ggccggcatc gccatggcga cgaccatcgt ctcgatcctc accggcattc cgatcaggcg
     6361 caccatcgcc atgaccggcg aggtgacgct gcggggcagg gtgctgccga tcggcgggct
     6421 caaggagaag ctgctggcgg cactgcgcgg cgggctgaaa accgtcctca tccccgagga
     6481 caacgtgaaa gacctcgccg acattccctc caatgtgaag aacgggctgg agatcatccc
     6541 ggtctcgcgc atggacgagg tgctcaagca cgcgctgctg cgccagccta cgcccatcga
     6601 atgggacgag gaggcgcacg agaaggcggc cggcaaggcc gtcggcgaaa ccggggcaag
     6661 cgctccgcat tgacggacgg gtgaggctcc gccgctagag catgtcgcga ttaagtggat
     6721 accggttaat cgcagaaggc atgctctaac ttgttgatac cgatcacgtt atccgcattt
     6781 tgatgattcc atcaaaatgc ggcgtgatct aggccgccgc ccatgccggg tggcggcccg
     6841 agggccgtat ttccaaacca tgagcggggc ttggcgcggg gaggatctgg atcagccttg
     6901 cgctgggccc gaatgtcgcc agagcacttt cagtgaagtc acattcattg aaagttcttt
     6961 tgttcttgct ttatcgcatt ttcttgacgc gaaccggact ccacttcgct cgaaaatgct
     7021 ctggggccaa cgcttttgac cgcccctctt gccaatcccg gcaaaatcaa gtgaatggca
     7081 gcgaggggcg gttagctcag ctggttagag cggctggttt acacccagta ggtcgggggt
     7141 tcgaatccct catcgcccac cagattatgc cagtggccct cggagctgac cgttcggtca
     7201 ttcctcgggc attcctcgca accgggcctg cggccctgct cggcgtcgca tccgctcctg
     7261 accaaaggcc cacgatcatg acctgttggt catgtccgca ggccgtcggt atcactggat
     7321 cgtgccgcgc ttgaggccga agcccggaat gttgcagcgg cagccggtgc cgatcggcgt
     7381 cagataggaa ttgcaggcgc cgcgcgaggt gacgcagacg ttgcccatcg gctgacggcg
     7441 gggcggacga tagccccaat cgcgcggcgg cgggcgatcg tagcgatacg gatcgacccg
     7501 gcgcggcctg tcatagggat catagccacg gggtccgtca tagggatcat agcgcggcgg
     7561 agccggacga taggggtcat aatactgcgc agaggcagtg ccggcggcgg tcgccaggat
     7621 caggaccgag gcggcggcca agacgaaacg cttcatgacg ggtctcccga atacggtaaa
     7681 cggatgtttg ccccacccga cattaacgca ggctgagcgg aaattgcagc attttccgac
     7741 aggcgtccgc tgcgggcggc ccaagatgaa acgccgccgc gcgatcccgg gctccggatt
     7801 gttaagattg ccgaggcata aacgatgcat cggttctggc cgtgtgttgt tcggccagct
     7861 tcgtggtgtt gcgtagagta gcagtggcga ctgtttgagt gtgtgatgcg tctttcgctt
     7921 ctgacggcgt ttgttgcgta tgcgtctgtc gcggctctgg cccagccggc cagggccgcc
     7981 gacatgacct tttcaaccct gtcctatggc tcaagccggc aatgcgtgga tgactgtccg
     8041 cgaatcatgc aggcgagcgg tcagatcacc gcatcaacgc cacaggcgct gatcgatttt
     8101 gcccgtcgtg aagtggccag gcccgggctt atcaacgttc tgctgattca ttcgccgggc
     8161 ggcagcgtca atgcctcgat cgccatgggg cgggtgctgc gcaagatggg aacgacggtg
     8221 atcgttgccc gcgccatgga catccgcgaa cttccgaccg ccaccattgg ccggcgcgaa
     8281 cccggcgtcg cggaaaatct ccgcttcgtg tccggaacct gcgcctcggc ctgtgtctat
     8341 gcgctggctg gcgggctgaa gcgggtcgtt ccgcaggaaa gccgcatcgc cgtgcaccgg
     8401 atggccggca atatcgccaa ttttgacccg gcgacccgcg aattccagca gcggcagatc
     8461 tatgccggcg agacggaact caacgccctg acggcctata tctacgagat ggggatcagc
     8521 accgatctcg tcgacatggc cgaatcggtg ccgcatgaga atgcgcgtat cctgtctaac
     8581 cgcgagattc gcgcgctgcg gctgggccag ccgaaactgt agcggcaacg acgttagggg
     8641 aattcgcatc gcgggcgctt gacttgaaag cgtcgctgcc gtaacccccc ggtctcgctg
     8701 ctgctggcag cgaagcaatg cgcgggcgta gctcagtcgg ttagagtgcc ggcctgtcac
     8761 gccggaggtc gcgggttcga gccccgtcgc ccgcgccatt ttccccttgg cttgacaatt
     8821 gtatggtgcg ccgggctgcg ggcctgttcc ttggtcgcat tcgccccttg agaaccgggg
     8881 tcctcgcttg cgtcgcatgc gtgggtgggc taagcggagc cgaggcacgt tgcccgcgcc
     8941 gggtcttttc ccggtttgcc gccaaggatg caccatgcag ctcttgctcg ccgattactt
     9001 tccgcttgtc gtcttcatcg gcctgtcggc cgttatcggc ctggcgctgc tggccgcgcc
     9061 gttccttctg gcgttcaagg cgccggatgc cgagaagctc tcggcctatg aatgcggctt
     9121 caacgccttc gacgatgcgc gcatgaagtt cgatgtgcgg ttctatctgg tggcgatcct
     9181 gttcatcatc ttcgatctgg aagttgcgtt cctgttcccc tgggcggttg cggcgcagga
     9241 gatcggctgg ttcggcttct ggtcgatgat gctgttcctg ggcgttctga cgatcggatt
     9301 catctacgag tggaacaagg gggcgctgga atgggactga cgccggatcg cgtcatggtc
     9361 tgtccagcac tggaggtttg agaagatggt ctcagcagtt gatcgcggca atccgcttgt
     9421 tgctcccgcg ccgcgcggtc tggtcggggc ggacgggcgc ccgatcggtt cgaccgatcc
     9481 gtatttcgcc accatcaaca acgaattggc ggacaagggc ttcatcgtca ccgccaccga
     9541 cgagttgatc gcctgggcac gcaccggctc gctgatgtgg atgaccttcg gccttgcctg
     9601 ctgcgcggtc gagatgatgc aggcatcgat gccgcgctat gatgtggaaa ggttcgggtt
     9661 cgctccccgc gcgtccccgc gccagtcgga cgtgatgatc gtcgccggca cgctgaccaa
     9721 caagatggcg ccggcgctgc gcaaggtcta cgaccagatg cccgagccgc gctacgtgat
     9781 ctcgatgggc tcctgcgcca acggcggcgg ctactaccac tattcgtatt cggtggtgcg
     9841 cggctgcgac cgcatcgtgc cggtcgacat ctacgtgccc ggctgcccgc cgagcgcgga
     9901 agcgctgctc tacggcatcc tgctgctgca gaagaagatc cgccgcacgg cgacgatcga
     9961 gagatgaccc cgatggcggc cgaacccaag gacatgatcg ttccgatgct caaggaaatg
    10021 cgggcggaaa acgcggcgct ccatggggat acgcgcaagc gtctcgataa gattgagcaa
    10081 gcgaaaaaag gtttcaggaa tgcgctcgcg gcagattcgc tcatgagcaa gatgatgatc
    10141 ggcgactggg aagagcgcat tgaggcgctc gaaaagaagg ttcgcaagct ggaatcctca
    10201 aaatgaccga caagcttcag gccctcggcg agcatatcgc cgcttcgctg cccggctctg
    10261 tgaccgggtt cgacgttgcc ttcggcgatc tgaacgtcac ggcgcgcgcg gccgacatcg
    10321 tcaaggtcat gaccttcctg caaggcgatg cgcgctgcct gttctggtcg ttcatcgacg
    10381 tctgcggcgt tgactacccg ggccgcgaca accgcttcga tgtggtctat cacctgctca
    10441 gcccgcgcca tgtccagcgt gtccgcgtca aggtcgagac cgacgaggcg accccggtgc
    10501 cgtcgatcac cggcctgttt cccggcgccg actggttcga gcgcgaggcc tacgacatgt
    10561 atggcatcct gttctcggac catccggacc tgcggcgcct tttgaccgac tacggcttcg
    10621 aggggcatcc cttgcgcaag gatttcccga ccacgggtta tgtcgaggtg cgctacgacg
    10681 atcaggaaaa gcgcgtcgtc tacgagaagg tgaagctcaa tcaggaaatg cggcagttcg
    10741 atttcctctc tccatgggag gggacggact acgtgctgcc cggcgatgag aaggccaagc
    10801 agtgatggct tcggcaatcg gcaatcggca gtcggcaatc gtgaggggga ctgcccttgg
    10861 ctatccagtc gcatcgcgat ctgcgcgtat ggaacgctgc ggtggagctg tccgtcgcct
    10921 gctaccggtt gacggcggat tttccgaggg aggaagccta tgggctgacc tcgcagattc
    10981 ggcgtgccag cgtgtcaatc gccgccaata tcgccgaggg ctacgggcgg gacaacgtgg
    11041 gttcgtatat ccagtttctc cggatcgcgc agggctcgtt gaaggaactc gaaaccttgc
    11101 tgcttcttgc cgagcgaatt ccgttgtgcg acagccggca gatcgtgccg ctcacccaac
    11161 aatccgacgg gatcgggcgc atgctgcgtt ccctgatccg cacattgcag gaccctgcat
    11221 gatgcattcc gattgccgat tgccgactgc cgattgccgt ttccagccgc gaggccgcgc
    11281 atgaccaccg cccccgatac ccccgcccac ctccgcaatt tccagatcaa ctgggggccg
    11341 cagcacccgg cggcgcatgg tgtgctgcgc cttgtgatgg aactcgacgg cgagatcgtc
    11401 gagcgctgcg atccgcatat cgggctcttg caccgtggca ccgagaaact gatcgagcag
    11461 aagacctatc ttcaggcggt gccgtatttc gaccggctcg actattgcgc gccgatgaac
    11521 caggagcatg ccttctgtct ggccatcgag cgactgctcg gcgtcgaggt gccgcgtcgt
    11581 ggccagctca tccgcgtgct gttctccgag atcggccgca tcctgtcgca tctgctcaac
    11641 atcacgacgc aggcgatgga cgtcggcgcg ctgaccccgc cgctgtgggg tttcgaggag
    11701 cgcgagaagc tgatggtgtt ctacgagcgg gcctgcggcg cccgcctgca cgccaactat
    11761 ttccgtcccg gcggcgtgca tcaggacctg ccggaggatc tgatcaacga tatcgaggcg
    11821 tggtgtgatc cgttcctcaa ggtggtgcag gatctcgacg atctgctgtc ggaaaaccgc
    11881 atcttcaagc agcgcaacgt cgatatcggc tatgtgtcga tcgaggatgc gtggaagcgt
    11941 ggcttctctg gcgtgatggt gcgcggctca ggcgccgcct gggacctgcg ccgcgcgacc
    12001 ccgtatgagt gctactcgga aatggaattc gacattccga tcggcaagaa cggcgattgc
    12061 tacgaccgct accacatccg catggaagag atgcgccagt cggtgcggat catgcgccag
    12121 tgcatcgtca agatgcgcca gagcgacggc aaggggccgg tgatggccgg cggcaaggtg
    12181 tcgccgccca agcgcggcga aatgaagcgc tcgatggaag cgctgatcca ccacttcaag
    12241 ctctataccg aaggcttcaa ggtgccggcc ggcgaggtct atgcggcgat cgaggcaccg
    12301 aagggcgagt tcggcgtcta tctggtggcg gacggcacca accgggccta tcgctgcaag
    12361 atccgcgcgc cgggctatgc gcatcttcag gcaatggatt tcatgaccaa gggccacatg
    12421 ctggcggacg tgtctgccgt catgggctcg ttcgatatcg tgttcggcga ggtggaccgg
    12481 taaggcgcgg acagcggggc aggggtgatg tttgctgcag gcggaacaac ggcggctttg
    12541 ctggccgggg cccttcaggc ccaggcggaa gtgccgtcgc cgcgcgggcc ggctccgccc
    12601 atcgtcacgc tggaacagct cgcgcgcgag ggctatgagg tgaaggcgat ccagagcgcg
    12661 gcaacgcgcg ggttcggctt cgtggtgatg ctgcagcggg gtggcgaggt gaagagctgc
    12721 ctgatgcgca tcgagcggaa tgcagacggc cgccccgcgc ggcaatctgt gtgtttttga
    12781 aaggtttgaa ccatgtcagt tcgccgcctc gcacctgatg cgatccagcc cgcgagcttt
    12841 acgttttcgg ccgaaaaccg ggcgtggatc gtcacgcagc ttgagaaata tccggccggc
    12901 cgtcaggcgt cggcggtgat cccgttgctg tggcaggcac agaagcaggc tggtggctgg
    12961 ctgccgcgcg ccgccatcga acatgtcggt gacctgctcg gaatgcccta tatccgcgtg
    13021 ctggaagtgg cgaccttcta cacgatgttc aacctcgaac cggtcggcaa gtatttcatc
    13081 cagctctgcg gcaccacgcc ctgcgccctg cgcggcgcgg aaaagatcat caaggtgtgc
    13141 aaggaccgca ttggcgagca gcgccacgtc accgccgacg gaatgttctc ctggctggaa
    13201 gtggaatgtc tcggcgcctg ctgcaacgcg ccgatggtgc agatcaacga cgatttctac
    13261 gaggatctga cgccggaaaa tttcaacaag ctgctcgatg atctggccgc cggccgcaag
    13321 gtcaaggtcg gctcgcagtc cggccgggtc tcgtccgagc cggaaggggc ggtcaagaca
    13381 ctgctcgacg ccgggcttta cgatggttct gccgtcggca actggcgcaa ggggttcgat
    13441 gagcgctaca gcaaggcgca ggcagccaag gctgccgctg cggctgctac ggccccggcc
    13501 gctccgccgc cggccgccaa gccggtc
//
LOCUS       DAOMXG010000053        13439 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_2520_length_13439_cov_12.370218, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000053 DAOMXG010000000
VERSION     DAOMXG010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 13439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13439
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2520_length_13439_cov_12.370218"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..1376)
                     /gene="hisD"
                     /locus_tag="PLQ11_05335"
     CDS             complement(57..1376)
                     /gene="hisD"
                     /locus_tag="PLQ11_05335"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HRK24358.1"
                     /translation="MIRYLKTARSEAERAEDDAKVRATVEGILADISTRGDAAVRELS
                     VTFDKYDPPSFRLTEQQIETAMSRVAKRDLEDIRFAQAQVRSFAEHQKASMRDIEVET
                     MPGVILGHRNIPVQSVGCYVPGGKYPMVASAHMSVVTAKVAGVKRIIASAPPQASADG
                     PAPHPAIVAAMHMGGADEIYCLGGIQAVGAMALGTQTIAPVDMLVGPGNAFVAEAKRQ
                     LYGRVGIDLFAGPTETMVIADDTVDGELCATDLLGQAEHGVNSPAVLITNSEKLARDT
                     MAEIERLLTILPTAGAAAQSWRDYGEVILCNSHEEMLSEANRIASEHVQVMTDRDDWY
                     LANMHSYGALFLGPRTNVSYGDKVIGTNHTLPTKRAGRYTGGLWVGKFLKTHTYQKVL
                     TDEASALIGAYCSRLCMLEGFVGHAEQANIRVRRYGGRNVPYGQAAE"
     gene            complement(1396..1932)
                     /locus_tag="PLQ11_05340"
     CDS             complement(1396..1932)
                     /locus_tag="PLQ11_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009466712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HRK24359.1"
                     /translation="MAGFRPEQAALTRDNDSSKTEATRRVIEGMVDGLNDHKINGMAE
                     FFHAGFRWFGNVGCGTKNGLREFQDNWQRPFQAAFSDKVCIDEARLFDGEWAASFGRQ
                     EATHSGEFMGIPPTGRRVQIRYMDFWKVVDGKIADNWVMVDFPHVLAQLGVDIFQSQG
                     WEAYDRGERVPPRPSHDK"
     gene            complement(1937..2866)
                     /locus_tag="PLQ11_05345"
     CDS             complement(1937..2866)
                     /locus_tag="PLQ11_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRK24360.1"
                     /translation="MATTRTLAHPLWLKLTSNGFLMLWLVAAIFPLLWITLMSFRAPV
                     DALSAHPLDVIFGKTTFDHYKAVWVTHGFWRNFLNSLMITAGVVTISLTVGTLAGYAL
                     ARANSNLAFWLLIMALVFRALPHSVLVTGYLPPFLNSRELLAPLWESGLTGWLFRLFS
                     DMPPTLYGQPWAIILVLVAINQPFTIWMMRSFFMNIPTELDEAARVDGCTHFGAFWRV
                     IMPVMWPGVITTGLFSFLLAYNDYLVTALLLDAQSQTMVPAISQFLNRDTRASDQIEA
                     IAAAVSITAPLFLLVMVFQRQIVSGLTAGAVKG"
     gene            complement(2866..3900)
                     /locus_tag="PLQ11_05350"
     CDS             complement(2866..3900)
                     /locus_tag="PLQ11_05350"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRK24361.1"
                     /translation="MKGRTFALFVAPSLAMMLIFIALPLASVFWQSFHETRAVVTRTE
                     VESCTPGFLAPTCTRETRTVPRIGADGRPVTETVFVGLDVYRRVLQTDAVAKALGAGG
                     GGLSAILGIDFWKALRFTLTFTLITLPLVIGLGLLIALAVNNLVKALRGPAIFITLLP
                     FIITPVIGALSIRWLFLGDGILTRAIEWVLARDISMFAQGWTIEIMMLIYKVWHVAPF
                     AFVVFYAALQTVDQDTLESAIVDGASRLERLRYVVIPHLMPLIVFVALIHLMDSYRVF
                     DEIVGFSAQAHVLSLQYLTYSFLTPDETGNRAVARAAASSMLTMIGIVILLVPLLMRT
                     WRTHRSARRG"
     gene            complement(3943..5157)
                     /locus_tag="PLQ11_05355"
     CDS             complement(3943..5157)
                     /locus_tag="PLQ11_05355"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRK24362.1"
                     /translation="MIRKTLAAAALSLMMSVSAQAACGFQNTVPLKSLTAAFEAWKAV
                     TSAMAECGNVQPELDQTFRTKQPAAFAANPSLYHIGGVANGTLTPLVNAGTIRPLDDL
                     IAKYGQNLSPNQLIKVDGKTVAVAMMVNAQHLVVREDILKELGIATPTTYDEVLAAAD
                     KIKASGKVQYPLGATFKAGWDLAQDFNNMFVGFGGTFIKPDNTPGMNSEAGVKALEMM
                     KKLSAYMDPEFLVSESTFVQKQLQQGKIAMANLWASRLGALDDPKESQHVGKFGVAAA
                     PKAMPGGPSSSTLWWDGIVIAKNISDAEAEAAFRVAMEGIDEEMVKANNGLAVWLIKG
                     YTPNKMAQGAIETARGGAPNYASTTAMGLFHSAVGEKIADFMAGRKDAKATLAEMETS
                     YLVKAKEQGLVK"
     gene            complement(5211..6284)
                     /gene="ugpC"
                     /locus_tag="PLQ11_05360"
     CDS             complement(5211..6284)
                     /gene="ugpC"
                     /locus_tag="PLQ11_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018067044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HRK24363.1"
                     /translation="MVAVSLRGITKRWGAFTGVSAIDLDIGDREFIVFLGPSGCGKTT
                     TMRMIAGLEDPTDGEIRIGDRIVNDLEPKDRDVAMVFQNYGLYPNMTVYENIRFPLKV
                     RKVDPATHDARVRKAAAMVELGDLLHRRPKELSGGQRQRVALARAIVREPAVFLMDEP
                     LSNLDAKLRVSTRAQIKNLQHELKVTTIYVTHDQIEAMTLADRVVVMNKGVFQQVGTP
                     TEIYDRPANTFVASFIGSPAMNLINGDIADGVFTAAQVRIAGLPSGLAGPVTLGFRAE
                     DAEVAAEGDGQIVAQIYAIELLGDATMVSVRVGDALVAAKAPKTYRAIIGDRVVFRIA
                     PAQCHLFDHQSGQRIEAKVDAGH"
     gene            6419..7498
                     /locus_tag="PLQ11_05365"
     CDS             6419..7498
                     /locus_tag="PLQ11_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009464707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HRK24364.1"
                     /translation="MTDQDRIIDAKRFVRAALSRVAEATPGTVLQRLTEAYHPQAEWR
                     GSYPMNEMSGFEAMAATVWQPLLRSFPDLERRDTLLMGGHWNGGDWVGAVGHYCGTFR
                     SDWLSIPATGRPIYIRYGEFHRVQDGRIAQSTVLIDVLDVIRQAGFWPIAPSLGTEEQ
                     WPCPFTGDGIVLTPQDPALSAASLAQTLAMHKTLGDYNDLDHRGREGLLDMPQKLHWH
                     PKMMWYGPCGIGTTRGLQGFVDYHQLPFRIAFPNRRGGNHYVRIGDGAYSATAGWPSV
                     HARHLGGDFCGVGPTGRDVTMRVMDFYLHHEGLIRENWVPIDIIHLLRQMDVDVFARM
                     QSFFRRGRIGTPRSRRILMESSKCG"
     gene            complement(7575..8582)
                     /locus_tag="PLQ11_05370"
     CDS             complement(7575..8582)
                     /locus_tag="PLQ11_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007199017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRK24365.1"
                     /translation="MNKSAVTSSDVAGLAGVSQSAVSRAFSPGASIAPETRNKVLRAA
                     EKLGYRPNALARAMISGRSRLVAVLVAYLDNQFYPLALEQLSRELQARGYQILIFMTD
                     TGNQDTVVQKMLQYQVEAIIMLSAELSSSLARECAATGTPVLLFNRYVPGLPASSVTS
                     DNLEGGRQVAHFLADGGHARIAFIAGQENSSTSRDREAGFCKGLAERGITPVARAVGG
                     YRFEGAAAAARELFAAAGRPDAVFVANDHMAFAVMDVLRGELGLRVPQDVSVVGYDDV
                     PEASWGGYDLTSVSQNLGEMIQATIDILISQIDRQQVKRKAAIIPAKLVVRGSARPTR
                     R"
     gene            complement(8656..9567)
                     /locus_tag="PLQ11_05375"
     CDS             complement(8656..9567)
                     /locus_tag="PLQ11_05375"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRK24366.1"
                     /translation="MPDISSNKIRALDGTSLLVMIELLQHRRTTIVADRMGLTQSAIS
                     QTLRRLRTLFDDALFLRRPHGLEPTAFALELEPKIRRIIDLMQESVGPPRAFDPAASQ
                     RTVRIAASEYELASLVPRFLALAEEEAVQMRVFGLPLRRDEGLARLADGTIDLVLGFY
                     WDAGSEFVTAPLYQEHYEVVMRRGHPLTGRLTSPRAFAEARHLLVTPDGEQRGLVDEA
                     LASEGLTRHVVATVPLFLPALASLAVSDLVACLPSRIAALFARQFDLQHQALPFSTRS
                     FPVSAVFHRRNANSPMHEWIIRRIESL"
     gene            9655..10863
                     /locus_tag="PLQ11_05380"
     CDS             9655..10863
                     /locus_tag="PLQ11_05380"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRK24367.1"
                     /translation="MKTTRPLLAALAALLLASAPAIAGCAFENKVPVKVSAAAFPAWK
                     AVTSAMAECGNVQSELSQEFQDKQAAGFAARPSLYHIGGVANSSLVQLVNQGLVHPLD
                     DLVARFGQKLRPNQLIRVDGKIVAIAMMANAQHLMYRKDIFDALGIAVPETYEDLLAA
                     ADRIQKANPVKYPFGGTWKTTWLEFVNVYMGYVGEFFTDRVQPAINNPKGIAALEMLR
                     KLTAYMDPEYLVSDATYVQQQFQQGRIAMANYWASRAAALDDPKELQVVGKVAMAAAP
                     APEKGLLPASTLWWDGIVIARNISYAEAEAALKVTLEGMDTDMVKASNSVAIGLIEGY
                     AADKLATGAQATAAKGAKPYPASTQMGLMQKAIANKLNDILKGSKDARTTLADIEADY
                     RVQAKEAGLL"
     gene            10904..11932
                     /locus_tag="PLQ11_05385"
     CDS             10904..11932
                     /locus_tag="PLQ11_05385"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HRK24368.1"
                     /translation="MTVHAVEASRLQIQKAKALVWDLWKAWQTVTPVPAQLPEAAMLD
                     EAVAFHGPAPLGLLTGRDQVLRQVYAPIANAFPLSRREPHLFLGGQFEGHVWVATTGY
                     ISGAMAGAWLGIPAGSGERRLRFGEFYRIENGRIVEIRCLFDILGLAAQAGYQLLPPF
                     QGRAEPPPGPALGNGIALGEQDPEATRYTLQLVEDMLGGCNRLNGSDLSSMGMAAYWH
                     EDMVWHGPWGVGSSYGFREFQEFAQGPSVASFPNRRGGFHRARFADGLTAAFTGWPSL
                     RGDFTGKPFRGIAPTGGPIGQNIMDFYVRRDDRLHENWILIDLIDFARQCGVDLLAPL
                     QRSPAARP"
     gene            11929..12360
                     /locus_tag="PLQ11_05390"
     CDS             11929..12360
                     /locus_tag="PLQ11_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24369.1"
                     /translation="MKTRTQLAFIAPSVVMMLVFIAAPLITVFWQSFHVSQPVFETVR
                     VENCTPAFLSQVCTTEEKTRPVLGPDGKMLMRSSFVGFSSQAHVISLQWLTYDFLKPD
                     DSGNRSISRASASAMLTMVGVIVLLAPLLRRTWADFRGNRP"
     gene            12678..13205
                     /locus_tag="PLQ11_05395"
     CDS             12678..13205
                     /locus_tag="PLQ11_05395"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRK24370.1"
                     /translation="MPHSVLVAGYLPWFINSADLLRPLLGDAAPTLYGKPWAVIAVLV
                     AINQPFTIWLLRSFFQSIPTELDEAARVDGCTHFQTFRFVIMPVMWPGVLTAGLFSFP
                     LGYNDYLVTSLLLDAQNQTMVPAVTSYFNAETTMRDQVEAVAAAVSITAPLLLLVLVF
                     QRQIVSGLTAGAVKG"
BASE COUNT         2475 a         4298 c         4248 g         2418 t
ORIGIN      
        1 ggtgaccagc gcgcgccggc catcgagccg gaacgagggg gtgcgcggca gcgccatcac
       61 tcggccgcct ggccataggg cacattgcgc ccgccataac ggcggacacg gatattggcc
      121 tgttcggcat ggccgacgaa cccttccagc atgcacagcc gcgagcagta ggccccgatc
      181 agcgcggagg cctcatcggt cagcaccttc tgataggtgt gggtcttgag gaacttgccg
      241 acccagagcc cgcccgtgta gcgcccggcc cgctttgtcg gcagcgtgtg gttggtgccg
      301 atcaccttgt cgccatagct gacattggtg cggggcccga ggaacagcgc cccgtaggag
      361 tgcatgttgg cgagatacca gtcgtcgcgg tcggtcatca cctgcacatg ctcggaggcg
      421 atgcgattgg cttcggagag catctcctca tggctgttgc agaggatcac ctcgccatag
      481 tcgcgccagc tctgtgccgc ggcgcctgcc gtcggcagga tggtcagaag ccgctcgatc
      541 tcggccatcg tgtcgcgcgc cagcttctcc gaattggtga tcagcaccgc cggcgaattg
      601 acgccatgct cggcctgtcc gagcagatcc gtggcgcaga gttcgccatc caccgtgtcg
      661 tcggcgatca ccatcgtctc ggtcggcccg gcgaagagat cgatgccgac gcggccatag
      721 agctggcgct tggcctccgc cacgaaggcg ttgcccggcc cgaccagcat gtcgaccggc
      781 gcgatggtct gggttcccag cgccatggcg ccgacggcct ggatgccgcc gaggcaatag
      841 atttcatcgg cgccgcccat gtgcatcgca gccacaatcg ccggatgcgg cgcgggacca
      901 tcggcgctcg cctgcggcgg ggcggatgcg atgatgcgct tcacacccgc gacctttgcg
      961 gtgaccaccg acatgtgggc ggaggccacc atcgggtact tgccgcccgg cacatagcag
     1021 ccgacggact gcaccgggat gttgcggtgg ccgaggatga cgcccggcat cgtctccacc
     1081 tcgatgtcgc gcatcgacgc cttctggtgc tcggcaaagc tgcgtacctg cgcctgcgcg
     1141 aagcggatat cctccaggtc acgcttggcg acccggctca tcgccgtctc gatctgctgc
     1201 tcggtcagcc ggaacgacgg cggatcgtac ttgtcgaacg tcacggagag ctcgcgcacg
     1261 gctgcgtcgc cgcgcgtcga gatgtcggcc aggatgccct caaccgtcgc gcgaaccttc
     1321 gcgtcgtcct cggcccgttc ggcctcactg cgggcggtct tgagatagcg gatcatcgct
     1381 gcggtcctgt gtcagtcact tgtcatgaga agggcgcggc ggcacgcgct cgccgcggtc
     1441 ataggcttcc cagccctggc tctggaagat atcgacgccg agttgcgcca gcacatgggg
     1501 gaaatcgacc ataacccagt tatcggcgat cttgccgtcg acgaccttcc agaaatccat
     1561 gtagcggatt tgaacgcgcc tgccggtggg cgggatcccc atgaactcgc ccgaatgcgt
     1621 ggcctcctgc cgcccgaagg acgccgccca ctcgccgtcg aacagccgcg cctcgtcgat
     1681 gcagaccttg tcggagaacg cggcctggaa ggggcgctgc cagttgtcct ggaattcgcg
     1741 gaggccattc ttcgtgccgc agccgacatt gccgaaccag cggaagccgg catggaagaa
     1801 ctcggccatg ccgttgatct tgtggtcgtt caaaccgtcg accatgccct cgatgacgcg
     1861 gcgcgtcgcc tcggtcttgc tgctgtcatt gtcgcgggtc agcgcagcct gttcgggtcg
     1921 gaatccggcc atggtcctat cccttcacgg cgccggcggt caggccggac acgatctgcc
     1981 gctggaacac catcaccagc aggaacagcg gcgccgtgat cgagacggcg gcggcaatgg
     2041 cctcgatctg gtccgaggcg cgagtatcgc ggttgaggaa ctgcgagatc gccggcacca
     2101 tggtctgcga ctgcgcatcg agcagcagcg ctgtgacgag gtagtcgtta taggcgagca
     2161 ggaacgagaa cagcccggtc gtgatcacgc ccggccacat caccggcatg atgacgcgcc
     2221 agaacgcacc gaaatgcgtg cagccatcga cgcgggccgc ctcatccagc tcagtgggga
     2281 tgttcatgaa gaaggagcgc atcatccaga tcgtgaaggg ctggttgatg gcgaccagaa
     2341 ccaggatgat cgcccagggc tggccgtaga gcgtgggcgg catgtccgag aacagccgga
     2401 agagccagcc ggtcaaaccg ctctcccaca agggtgccag caattcgcgc gaattgagga
     2461 agggcgggag atagcccgtc accagcaccg aatgcggcag cgcccggaac accagcgcca
     2521 tgatcagaag ccagaaggcg agattggagt tcgcccgcgc cagcgcatag cccgccagcg
     2581 tgccgaccgt gagcgaaatc gtgacgacac ccgccgtgat catcagcgag ttgagaaaat
     2641 tgcgccagaa gccatgcgtg acccagaccg ccttgtagtg atcgaaggtc gtcttgccga
     2701 agatcacatc gagcggatgg gccgagagcg catcgaccgg cgccctgaac gacatcagcg
     2761 tgatccagag cagcgggaag atcgctgcca ccagccacag catcaggaaa ccgttgctgg
     2821 tcagcttcag ccagagcgga tgggccaggg tgcgtgttgt cgccatcatc ccctcctcgc
     2881 ggagcgatgc gtccgccagg tccgcatcag caagggaacg agcaggatga cgatcccgat
     2941 catcgtcagc atggaggagg cagcggcccg cgcgaccgca cggtttccgg tctcgtccgg
     3001 cgtcaggaac gaataggtca ggtattgcag cgagagcaca tgggcctgcg ccgagaaccc
     3061 gacgatctca tcgaagacgc gataggaatc catgagatgg atgagcgcga cgaagacgat
     3121 cagcggcatc agatgcggga tcacgacata gcgcagccgc tccagccgcg aggcgccgtc
     3181 gacaatcgcg gattcaagtg tgtcctgatc gacagtctga agtgcggcat agaacaccac
     3241 gaaggcgaag ggcgcgacat gccagacctt gtagatcaac atcatgatct cgatcgtcca
     3301 gccttgcgcg aacatcgaga tgtcgcgcgc cagcacccat tcgatcgccc gcgtcaggat
     3361 gccgtcgccg agaaacagcc agcggatcga caaggcgccg atcaccggcg tgatgatgaa
     3421 gggcagcagc gtgatgaaga tggccggacc tcgcaacgcc ttgacgaggt tgttcaccgc
     3481 gagcgcaatc aggaggccga ggccaatcac cagcggcagg gtgatcagcg tgaaggtgag
     3541 ggtgaagcgc agcgccttcc agaaatcgat gccgaggatg gccgacagcc cgcctccgcc
     3601 agcgcccagc gcctttgcca ccgcatccgt ctgcagaaca cgccgataga catcgagccc
     3661 gacaaagacc gtttccgtga caggccttcc gtcagccccg atcctgggca ccgtccgggt
     3721 ttcgcgtgtg caggtcggcg cgagaaagcc gggcgtgcag ctttccacct cggtccgggt
     3781 cacgaccgca cgcgtctcgt ggaagctctg ccagaacacc gaggccaggg gcagcgcgat
     3841 gaagatcagc atcatcgcaa gcgacggcgc gacgaacaat gcgaaggttc tgcccttcat
     3901 cgccgcgccc tctttcttgc cgtccggtcc tgacaggatg cgttacttca cgaggccctg
     3961 ctccttcgcc ttgacgagat agctcgtctc catctccgcc agcgtggcct tcgcatcctt
     4021 acggccggcc atgaaatcgg cgatcttctc gccgacggcg gagtggaaca ggcccatggc
     4081 cgtggtggaa gcatagttcg gcgcgccgcc cctggcggtc tcgatggcac cttgcgccat
     4141 cttgttcggc gtgtagccct tgatgagcca gacggcgagg ccgttattgg ctttcaccat
     4201 ctcctcgtcg atgccctcca tcgcgacgcg gaaggccgcc tcggcctcgg catcggagat
     4261 gttcttggcg atcacgatcc cgtcccacca cagtgtcgag gaggacgggc cgcccggcat
     4321 cgccttcggt gcggccgcca cgccgaactt gccgacatgc tggctctcct tcggatcatc
     4381 gagcgcgcca agccgcgacg cccagagatt ggccatcgcg atcttgccct gctggagctg
     4441 cttctggacg aaggtcgatt ccgaaacgag gaattccggg tccatatagg ccgagagctt
     4501 cttcatcatc tcgagcgcct tcaccccggc ctcggagttc atccccggcg tgttgtccgg
     4561 cttgatgaag gtgccgccga agccgacgaa catgttgttg aagtcctgcg ccagatccca
     4621 gccagccttg aaggtggcgc ccagcggata ctgcaccttg cccgaggcct tgatcttgtc
     4681 ggccgcagcc agcacctcgt cataggtggt cggcgtcgcg atgccgagtt ccttcaggat
     4741 atcttcgcga accacgagat gctgcgcgtt gaccatcatc gcgacggcga ccgtcttgcc
     4801 atcgaccttg atgagctgat tgggcgacag gttctggccg tacttggcga tcagatcgtc
     4861 gagcgggcgg atggtgccgg cattgacgag cggcgtcagc gtgccattgg cgacgccgcc
     4921 gatatggtac agcgacggat tggccgcgaa ggccgccggc tgcttggtgc ggaaggtctg
     4981 gtccagctcc ggctggacat tgccgcattc ggccatggcg gacgtcaccg ccttccaggc
     5041 ctcgaaggcc gcggtcagcg acttgagcgg cacggtgttc tggaagccgc aggcggcttg
     5101 cgccgacacg gacatcatga gcgacaatgc agcggcggcg agtgtcttgc ggatcatgct
     5161 ctctctccca aggcaggtgt ggcagtgact tcggcggaca ggacgcaggt tcagtgacct
     5221 gcgtcgactt ttgcttcaat ccgttggccg gactggtgat cgaacagatg gcattgcgcc
     5281 ggggcaatac ggaacacgac cctgtcgccg atgatcgcgc ggtaggtctt gggggccttt
     5341 gcggccacca gcgcatcgcc gacacgcacg ctgaccatcg tggcgtcgcc cagaagctcg
     5401 atcgcgtaga tctgcgcgac gatctgccca tcaccttcgg ccgcgacctc ggcatcctcg
     5461 gcgcggaaac ccagcgtgac cggccccgcc agcccgctgg gcagcccggc aatcctgacc
     5521 tgcgcggcgg taaagacacc atcggcgatg tcgccattga tcaggttcat cgccggtgag
     5581 ccgatgaagc ttgcgacaaa ggtgttggcc gggcggtcgt agatctcggt cggtgtcccg
     5641 acctgctgga acactccctt gttcatgacg acgacgcggt ccgccagcgt catcgcctcg
     5701 atctggtcat gggtcacata gatggtcgtc accttcagct cgtgctggag gttcttgatc
     5761 tgcgcgcggg tcgagacacg cagcttcgca tccagattgg agagcggctc gtccatcagg
     5821 aagactgccg gctcgcgtac gatggcgcgc gccagggcaa cgcgctgccg ctggccaccg
     5881 gaaagctcct tcggccggcg gtggagaagg tcgcccagtt cgaccatcgc ggcggccttg
     5941 cggacgcgcg catcatgggt ggcaggatcg accttgcgca ccttaagcgg gaaccggatg
     6001 ttctcgtaga cggtcatgtt cggataaaga ccgtaattct ggaacaccat cgcgacatcg
     6061 cgatccttcg gctccagatc attgacgatg cgatcgccga tccggatctc gccgtcggtc
     6121 gggtcctcga ggccggcgat catccgcatt gtcgtcgtct tgccgcagcc cgaggggccg
     6181 agaaagacga tgaactcccg gtcgccgata tcgaggtcga tcgcgctcac gccggtgaac
     6241 gcgccccatc tcttggtgat gccgcgcagg gaaacagcca ccatgcccga atgatctcct
     6301 ccagcgacct tttcgggccg cctctccgca gatgacactg acagagattg catacgtatg
     6361 caatagcctc tagtctaccc aagccgaaac cgtggacagc cgcttttccg gagatccgat
     6421 gaccgatcag gaccgcatca ttgacgccaa gaggttcgtg cgcgcggcgc tctcgcgtgt
     6481 ggccgaggcg acaccgggca ctgtgctgca aaggctcacc gaggcctacc atcctcaggc
     6541 ggagtggcgg ggctcctacc ccatgaatga aatgtccggc ttcgaggcga tggcggcaac
     6601 ggtctggcag ccgctgctgc ggtccttccc cgatctggag cgccgcgaca ccctgctgat
     6661 gggcggtcac tggaatggcg gcgactgggt cggcgcggtt ggccattatt gcggcacgtt
     6721 tcgcagtgac tggctgtcga tcccggcgac cgggcggccg atctatatcc gctacggcga
     6781 gttccatcgc gtccaggacg gcaggatcgc ccagagcacc gtgctgatcg acgtgctcga
     6841 cgtcatacgg caggcggggt tctggcccat cgcccccagt ctcggcaccg aggagcagtg
     6901 gccctgcccg tttaccggcg acgggattgt tctgaccccg caggacccgg ccctgtcagc
     6961 ggcgagcctc gcccagacgc tagccatgca taagacgctc ggcgactata atgaccttga
     7021 ccatcgcggc cgcgagggtc tgctggacat gccgcaaaag ctgcactggc atccgaagat
     7081 gatgtggtac ggaccctgcg gcattggcac gacacggggc ttgcagggct ttgtcgacta
     7141 ccaccagctg cccttccgga tcgcctttcc gaaccggcgc ggcggcaacc actatgtccg
     7201 catcggcgac ggcgcctatt cggcaacggc gggctggccg agcgtccatg cgcgccacct
     7261 cggcggcgac ttctgcggcg tcggcccgac cggtcgcgat gtcaccatgc gcgtgatgga
     7321 cttctacctc caccatgaag gcctgatccg cgagaactgg gtgcccatcg acatcatcca
     7381 cctgctcagg cagatggatg tcgacgtgtt tgcaaggatg cagagcttct tccggcgcgg
     7441 ccggatcggc acgcctagat cacgccgcat tttgatggaa tcatcaaaat gcggataacg
     7501 tgatcggtat caataagtta gagcatgttt tctgcgatta accggtatcc cttaatcgcg
     7561 acatgctcta gaggctagcg gcgggtcgga cgtgccgagc cgcggacgac cagtttcgca
     7621 gggatgatcg ccgccttgcg cttcacctgc tgccggtcga tctgcgagat caggatgtcg
     7681 atggtggcct ggatcatctc gccaaggttc tgggaaaccg aggtcaggtc atagccgccc
     7741 caggaggcct cgggcacgtc gtcatagccg acaaccgaga catcctgcgg aacacgaagc
     7801 ccaagctctc cgcgcagcac atccatcact gcaaaggcca tgtggtcatt ggcgacaaag
     7861 accgcatcgg ggcggccagc cgctgcgaac agctctcgtg ccgctgcagc ggccccttca
     7921 aagcggtatc cacccaccgc gcgggcaacc ggcgttatgc cgcgttcggc caggcccttg
     7981 cagaagccgg cctcgcggtc gcggctggtc gaggagtttt cctgcccggc gatgaaggcg
     8041 atccgggcat gaccgccatc ggccaggaaa tgcgcgacct gacgtccgcc ttcgagattg
     8101 tctgaggtca cgctgctggc aggcagaccc ggcacatagc ggttgaacag cagcaccggc
     8161 gtccccgtgg ccgcgcattc ccgcgccagg ctggacgaca gttctgccga gagcatgatg
     8221 attgcctcga cctgatactg cagcatcttc tggaccacgg tgtcctgatt gccggtgtcg
     8281 gtcatgaaga tcaggatctg atagccccgc gcctgcaact cgcgcgacag ctgttctagg
     8341 gcgagcggat agaattggtt gtcgagatag gcaacgagca cggcgaccag acgcgagcgg
     8401 ccactgatca ttgcccgcgc aagcgcattg gggcgatagc ccagcttctc ggccgcgcgc
     8461 agcaccttgt tccgcgtctc cggggcgatg ctggcgcccg gggaaaaggc gcggctgacg
     8521 gccgactgcg agacgccggc aagacccgcc acatcactgg aggtgacagc tgacttgttc
     8581 atgacggcgg caacctttgg catgatgaga tgtataccga catgcagcgc agtgatcgag
     8641 gttcaggcat cagcgtcata gggattcaat acgccgaata atccattcat gcatcggact
     8701 gttggcattg cgcctgtgaa acacagccga aaccggaaag gaccgcgtcg agaatggcag
     8761 cgcctgatgc tgcaggtcaa actgccgggc gaacagggcg gcaatccggc tgggcaggca
     8821 ggcgacgaga tcgctgacgg ccagtgacgc aagcgccgga aggaacagcg gaacggttgc
     8881 aacgacgtga cgggtcaacc cctcggaggc aagcgcttca tcgacgaggc cacgctgttc
     8941 gccatccggc gtgacgagca ggtgccgcgc ctccgcaaag gccctgggcg aggtcaagcg
     9001 accggtcagc ggatgcccgc ggcgcatcac cacctcgtaa tgctcctgat acaaaggtgc
     9061 ggtgacgaac tccgagccgg catcccagta gaacccgaga acaagatcga ttgtcccgtc
     9121 ggcaaggcgt gcaagaccct catcgcgccg gagcggcagg ccgaagacgc gcatctgcac
     9181 ggcctcctcc tcagccagcg ctagaaaacg cggaacgagc gaggccagtt catattcgct
     9241 ggccgcgatc cggacggtgc gctggctggc ggcaggatcg aaagcgcgcg gcgggccgac
     9301 cgattcctgc atcaggtcaa tgattctcct gatcttcggc tccagctcga gcgcgaaggc
     9361 cgtcggttcg agaccatggg gccgccgcag gaacaaggcg tcatcgaaca atgtcctcag
     9421 ccggcgcagg gtttggctga ttgccgactg cgtcagcccc atacgatcag cgacgatcgt
     9481 ggtgcgccgg tgctgcagca gctcgatcat caccagaagc gatgtcccgt cgagcgccct
     9541 gatcttattt gaactgatat ccggcatgac tcgatttcag ttttgtggat ttcgtccgct
     9601 gataccgtgc ggtccatgga ccggcaatcc ccatgctggc gcagggagga cgacatgaag
     9661 acaacacgac cgcttctggc cgctctggcg gccttgctgc tcgcctcggc acccgccatt
     9721 gcaggctgcg ccttcgagaa caaggttccg gtcaaggttt cagccgcagc cttcccggcc
     9781 tggaaggccg tgacctccgc catggccgaa tgcggcaatg tgcagtccga actcagccag
     9841 gagttccagg acaagcaggc cgcaggcttt gccgccaggc cctcgctata tcacatcggc
     9901 ggcgtggcca attcgtccct cgtccagctg gtcaatcagg gcctcgtgca cccgctcgat
     9961 gatctcgtcg cgcgcttcgg ccagaagctc cggcccaacc agctgatccg tgtcgacggc
    10021 aagatcgtcg cgatcgcgat gatggcgaat gcgcagcatc tcatgtaccg caaggacatc
    10081 ttcgatgcgc tcgggatcgc ggttccggaa acctacgagg atctgctcgc ggcagccgac
    10141 agaatccaga aggcgaaccc cgtcaaatat ccgtttggcg ggacctggaa gacgacgtgg
    10201 cttgagttcg tcaacgtgta tatgggctat gtcggcgagt tcttcaccga tcgtgtccaa
    10261 cccgccatca acaatccaaa gggtatcgcg gccctcgaaa tgctgcggaa gctgaccgcc
    10321 tatatggacc cggaatatct ggtgtccgac gcgacctatg tgcagcagca gttccagcaa
    10381 ggccgcatcg ccatggcgaa ttactgggcg tcgcgcgctg ccgcgcttga tgatcccaag
    10441 gagttgcagg tcgtcggcaa ggtggcgatg gccgcagccc ccgcgcccga gaagggcctg
    10501 ctgccggcct cgaccctgtg gtgggacgga atcgtgatcg cccggaacat ctcctacgcc
    10561 gaggcggaag ccgccctcaa ggtcaccctt gaaggcatgg atacggacat ggtcaaggcc
    10621 agtaattcgg tcgcgatcgg gctgatcgag ggctatgccg ccgacaagct tgccacgggc
    10681 gcgcaagcca ccgcagcaaa gggcgccaag ccctacccgg ccagcacgca gatgggcctg
    10741 atgcagaagg cgattgccaa caagctcaac gacatcctga agggcagcaa ggacgccaga
    10801 accacgctgg ccgatatcga ggcggactat cgcgtccaag ccaaggaggc cggtctgctg
    10861 tgaagcagaa cggccggccc tttcctcagc cgggcaccga tacatgaccg tccatgcagt
    10921 cgaggcatcg cgccttcaaa tccagaaagc caaggcgctg gtctgggatc tctggaaggc
    10981 ctggcaaacc gtaaccccgg tgccggcgca gttgccggag gccgcaatgc ttgatgaggc
    11041 cgttgccttt cacgggccag cgccgcttgg ccttctgacg ggacgcgatc aggttctgag
    11101 gcaggtctat gcgccaattg ccaatgcctt cccgctgtcg cgccgggaac cccatctttt
    11161 tctcggcggc cagttcgagg gccatgtctg ggtcgcgaca accggttaca tctccggcgc
    11221 catggccggc gcctggcttg gcattccggc aggttccggc gagcggcgtc tgcgcttcgg
    11281 cgagttctac cggattgaga acggtcgtat cgtcgagatc cgctgcctgt tcgacattct
    11341 gggtctggca gcacaggccg gctaccagct gctgccgccc tttcagggca gagccgagcc
    11401 tccgcccgga cctgcactcg gcaatggtat tgcactgggc gaacaggacc cggaggcgac
    11461 gcgttacacc ttgcagcttg tcgaggacat gctcgggggc tgcaaccgcc tcaatggcag
    11521 cgatctgtcg agcatgggca tggccgccta ctggcacgag gatatggtct ggcacgggcc
    11581 ctggggtgtc ggatccagct atggcttccg cgagttccag gagttcgcgc aggggccatc
    11641 ggttgcgagt tttcccaacc ggcgcggcgg ctttcatcgt gcgcgctttg ccgacgggct
    11701 gacggcggcc tttaccggct ggccgagcct gcgtggtgat ttcaccggca agccattccg
    11761 cggcattgcg ccgacgggcg gcccgatcgg ccagaacatc atggatttct acgttcggcg
    11821 cgatgacagg ctgcacgaga actggatcct gatcgacctc atcgacttcg cccggcaatg
    11881 cggcgttgat ctgcttgcac cgctccagcg ctcccccgct gcccggccat gaagacgcgg
    11941 acgcagctcg ccttcattgc gccctcggtc gtgatgatgc tggtcttcat cgctgccccg
    12001 ctgatcacgg tgttctggca gagctttcac gtcagccagc ctgtcttcga gaccgtccgg
    12061 gtcgagaact gtacacccgc cttcctctcg caggtctgca ccaccgagga aaagacccgg
    12121 ccggttctgg gaccggatgg caagatgctg atgcgatcga gcttcgtcgg cttctcgagc
    12181 caggctcacg tgatctccct tcaatggctg acctatgact tcctgaagcc cgacgacagc
    12241 ggcaaccggt cgatctcgcg ggcatcggcc agcgcgatgc tcaccatggt cggggtcatt
    12301 gtcctgctgg ccccgctcct tcgccggacc tgggcagact tccgggggaa ccggccttga
    12361 gcctcccgcg cgtcctgcgt cagccctggc cggcgcgctg gggcaaccgc tgatcggcgc
    12421 aacgaccgag cattacgaag cggtctgggt ccgcaatgaa ttctaccgga acttcctgaa
    12481 ctcgatgctc gtcacgggtg gtgtggtgct gatttcgctg acctgcggca cactggccgg
    12541 ctatgggctg tcgcgctcgg gcgcgagcat tgccgcgcag aagggcagca agaagggcgg
    12601 taaggccaaa tgggctgtcg cgctcgggcg cgagcattgc cttctgggtc ctgatcattg
    12661 cgctggtctt ccgggccttg ccgcattcgg ttctcgttgc cggatacctc ccctggttca
    12721 tcaactccgc cgacctgctg cggccgctgc tcggggacgc tgcgccaacg ctctacggca
    12781 agccctgggc cgtcatcgcc gtgctggttg ccatcaacca gcccttcacg atctggctgc
    12841 tgcgctcctt cttccagtct atcccgactg aactggacga ggccgcgcgc gtcgatggct
    12901 gcacgcattt ccagaccttt cgcttcgtca tcatgccggt gatgtggccc ggcgtgctga
    12961 ctgccggatt gttcagtttt ccgctcggct acaatgacta cctcgtcacc tcgcttctgc
    13021 ttgatgccca gaaccagacc atggtcccgg ccgtcacgag ctatttcaac gcggaaacga
    13081 cgatgcgcga tcaggtcgag gccgtcgctg cagccgtgtc gatcaccgcg ccgctgctgc
    13141 tgctggtcct ggtcttccag cgccagatcg tcagcggcct gactgcgggt gccgtgaagg
    13201 gttaggcgcg tcgaaactct tcgtcaaagc tgtccgtttg atccgaactg agcgttggca
    13261 gccaagcgcc ctggctgaat gacaacgggc atactgctag agcattttca agcgaagtgg
    13321 attccggttc gcgttaagaa aatgcgaaaa aacaaaaaga tagagcactt tctgtgaatt
    13381 ggaattcact gaaagtgctc taaggcgcgt ggggattcat cgtgaattga tgacggctg
//
LOCUS       DAOMXG010000054        13271 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_2565_length_13271_cov_8.813711, whole genome shotgun sequence.
ACCESSION   DAOMXG010000054 DAOMXG010000000
VERSION     DAOMXG010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 13271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13271
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2565_length_13271_cov_8.813711"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1272
                     /locus_tag="PLQ11_05400"
     CDS             <1..1272
                     /locus_tag="PLQ11_05400"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HRK24371.1"
                     /translation="ERALAAILADMATGERMLRLLQGDVGSGKTIVALLAMARAVEAG
                     RQAALMAPTEILARQHFDRLAPLAAAAGMRMALLTGRAKASERAAVLAGLAAGEIDLV
                     VGTHALFQDDVAFRDLALAVVDEQHRFGVHQRLALSRKGDATDLLVMTATPIPRSLVL
                     TYFGDMDVSILDEKPAGRLPIDTRLIDLDRLGEVAEGIGRAIASGARVYWVCPLVAES
                     ETSDLAAAEARRDDLARLLGDRVGLVHGRMKGTDKDEAVRRFQSGETAVLVATTVIEV
                     GVDVPEATIMVIEHAERFGLAQLHQLRGRVGRGAGRSTCLLLYKAPLGEVAGARLKIL
                     RESEDGFRIAEEDLRLRGEGDVLGTRQSGSPGFRIARLEAHGDLLRMARQDAELALNR
                     DPDLTGPRGAALRLLLYLFEREEAVRLFRAG"
     gene            1380..2156
                     /locus_tag="PLQ11_05405"
     CDS             1380..2156
                     /locus_tag="PLQ11_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRK24372.1"
                     /translation="MRKLTVLLVAAAAVAMTGAAQAQQAEWKEIRIGTEGAYPPFNNL
                     NAKKELEGFEIDYANALCEKMKVKCTWVVQDWDGIIPALLSKKYDVIVAGMNATEERA
                     KRVDFSMVYTKTPISMIAAKSVTSTDVSPAALKGKAIGTQGSTIHANFLEKHYKDSTP
                     RLYPTQEEANLDLLNGRLDYVVADKLALEDFINGKGKDCCKMIADVTRDPAIHGPGVG
                     MAVRKEDGALKALLNKAIEDSIKDGTHKKIADKWFKFPIL"
     gene            2261..2983
                     /locus_tag="PLQ11_05410"
     CDS             2261..2983
                     /locus_tag="PLQ11_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400553.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRK24373.1"
                     /translation="MDRYITLLSFGPEGWGAALLSGALVTISIALATLPFGLALGLAV
                     ALAKRSRHAWLRGPGTLYTTIFRGVPELLTLYIIYFGIQMLVQSLWTWLGFAGTIEMS
                     PFLAGVFALGIVLAAFSAEVWLGALNSVQRGQREAAAALGLTSWQAFRLVVFPQLFRV
                     ALPGLGNNWMVLLKETSLVSVITLQDTMFIATRANVVTKEPFLFFGVAMLIYLFFSAV
                     SAAGLAKAEARTNRGFAAPGAR"
     gene            2983..3699
                     /locus_tag="PLQ11_05415"
     CDS             2983..3699
                     /locus_tag="PLQ11_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909471.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRK24374.1"
                     /translation="MLWCEYPGAVLGIEVLQNYGCRMASGLGLTLQLVAISFGIGLIA
                     GLGLALLRLNAPRPLAWLVGAYMTVFRGTPLLGQLFLIYYGLGSFRLFWQDVGLWWLF
                     RDPLYCCLLAFTLNTAAYQAEIFRGALIALPRGQREAAMALGLHPVPTYLKVLLPQAL
                     ITALRPLGNELIVMIKASSLASLVTLFDLMGVTKLAFARSFDLTIYLVAALLYLVLVE
                     IIRRLWDNLERRLTRHLALA"
     gene            3696..4865
                     /locus_tag="PLQ11_05420"
     CDS             3696..4865
                     /locus_tag="PLQ11_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015343559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HRK24375.1"
                     /translation="MTAYSINPSVAAVEAPPIPSAQAWKRDYDGARGPMIDLTQAVPG
                     TPPPADVLERLAMAAGSIEATRYGPILGDSALRQAHAGETWRLYGGAIEPQHVAITCG
                     CNQAFVVTMLALARAGDAVILPAPWYFNHKMTLDMLGLETRVLACRPEAGFVPDPDAA
                     ERLIDGRCRALVLVSPNNPTGAIYPPQLLARFFDLCRRHRLALVLDETYRDFLPSDAA
                     PHGLLARSDWPDTLIQLYSFSKSHALPGHRLGAIVAAPATIGEIAKVQDSLQICPARP
                     AQGVVADSIESQRGWRAMRRDELIARGTAFQALFARQNRWRIDSLGAYFAFVAHPFTG
                     LDSVAAARRLALEAGVLGLPGSYFGPGLDGHLRLAFANVETAGLADLPQRLSAMA"
     gene            complement(4910..5521)
                     /locus_tag="PLQ11_05425"
     CDS             complement(4910..5521)
                     /locus_tag="PLQ11_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003601702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HRK24376.1"
                     /translation="MQLYYSPASPYARKVLIVAHETGLADTITLLTGPVSPIEKDKAI
                     NRHNPAGKIPTLVTEAGEAIYDSRVIVEYLAARAGDSKLFPAGAERWKALVLQSLCDE
                     ALDACLLARYEQVLRPEPLRWGAWIEGQMHKIATSLDVLERDYAHRLAAHFDIGTIAA
                     ATLMGYLDFRFTDFDWRATRPHLAAWAAGIVARPSVAATLPKA"
     gene            5775..5866
                     /locus_tag="PLQ11_05430"
     tRNA            5775..5866
                     /locus_tag="PLQ11_05430"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5809..5811,aa:Ser,seq:gct)
     gene            complement(6158..9367)
                     /locus_tag="PLQ11_05435"
     CDS             complement(6158..9367)
                     /locus_tag="PLQ11_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HRK24377.1"
                     /translation="MSGPIVISSSGVSLYDVAARYGVSIKDLYRLNPQFVPGSTDGKR
                     FDASVQQAMFERGQKGRDGDYLAVGEAINLPDGAPRHEPVTFYKADLSHARRQHVLEY
                     VRGKMTEAGLKGADTMGRDEFMALVRKADPKLTERQAQELFNETGDAQHKYAIWQDKI
                     LAGEVTAALAEDALLEAEERSVKSDKEARQILKNLERIEGNLNSLKRQVQENGARIDQ
                     TLASIKAEQAANDSWNARNGAINTDGSLKPWAKTGRDMFKTSSDRLEWARKALEAGVD
                     PNEVFASPGEAKAFMADKAMREVEGKYDGWIGSFSDGLADALPGVTSTLDARKQVDKL
                     TSLAFMRSSDPATLARQNALNAENSQQANAAVAKIAEADKHVGRTQTGVEVTAVVAVG
                     VATGGTGLLASTAITTGTTIGLHTLDDATNTGDTYGWNGVVRDVEFGVLGAAGGRALG
                     ALFKAPAAKVVSSGADDVAAAGNTVDDLAAAGSGASNQATETVVETATKKTAKELAQE
                     QQERSLLGRFDKLIDKRVEGTNTFFGISKSPFTKTADKAADGLVNATDDVVAMADDAV
                     QVADDAVRGVDDVVQTADDVSGAADDVAGNAATKSRTAGEVIESAQKEFELSWKNGKG
                     AKYVYTPGDLPYVPNLKFNPQYGLANVPQLVGEGVKILPRVVANPTGVIQRLATPGVE
                     LTRWASAKLYHPARDLVVDLAMEPGRQAMVQFGRTVAQSPAGQATSWAVSKTAGLLDD
                     VAGSRFGSFVGTTAQRPVNWLTRQYEGTLLQQGVNALPVRFSAAGGQLAGDFSTAVKG
                     TAEAAKGVGNVLNRVSGAAIGLGIADAWASDGPTVTHNNEFARAQYNMKKTSENITWA
                     APHLPVSVTLGWDKSLAVARLPAPLGSGAMPGADWNDGKGRARGLMNSSIGATEVLFG
                     FTVGGAEVNYSGAISMSPLRYMWNQMAYNNYDGVKSPGYAQHSLKTFGGITPHSIQFA
                     GKFNFGPAAIYLDRANSLSTIYTLATDPKVATEYGASMLTQQGSRVTAASKGDFIAIP
                     NTALTINTAYKAETARPLWKWYQNFLEPQPTETYTPDPR"
     gene            9655..11463
                     /gene="iorA"
                     /locus_tag="PLQ11_05440"
     CDS             9655..11463
                     /gene="iorA"
                     /locus_tag="PLQ11_05440"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HRK24378.1"
                     /translation="MNQQRRIEPDIVTDTLLPLMGNEAIARGAWEAGVRVAAAYPGTP
                     STEILESLGTYPASDVSAEWATNEKVALDVVIGAALCGVRALTAMKHVGLNVAADALM
                     SQSYIGSHGGLVLIVCDDPGIHSSQNEQDTRLFARLANVPVLEPSDAQEAYDFTKLAF
                     EISEQFDTPVIVRSTTRLSHTRSMVRVGVRSEVPGKGFVETSTKTVTIPANARRLHPK
                     VLEREARLARYFETSPLTRREAGETRFGIITASTAYLYVKEVAPHASVLKLGASHPLP
                     EAAIRAFAASVDRLLIVEELEPALEKDIRAMGIPAEGKAFFPRLGELSPELVQAGLAA
                     AGLMEPAAAVRSLNIEPMARPPVLCPGCPHTATYLALRGIGARVSGDIGCYTLAAVEP
                     LKSMDTAVCMGASIGNAIGMARSGVDTKPIVATIGDSTFLHSGIPPLIDAVYNRVNIT
                     VVILDNAITAMTGGQDHPGTGRSLRGEPTYKVDYEQICRAAGVDWVRTVDPYQVGHMY
                     QTLREAIVHRGVSVVIASRPCVLDPVKIKGTPLAINPAGCVACQACMNLGCPSLSWTE
                     EMHEGRHKVMIEAASCIGCTLCAQVCPSDCIQPVAP"
     gene            11460..12371
                     /locus_tag="PLQ11_05445"
     CDS             11460..12371
                     /locus_tag="PLQ11_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRK24379.1"
                     /translation="MSQPARSERANQARNIMIVGVGGQGVLMISRVMAMMGHRQGHQV
                     KQSEVHGMAKRGGVVFSHVRFGEAVWSPTIAKGEADALIALEWAEGLRWLDHLRPETG
                     VFIADTRRIVPPFACRNRRAGAATNYALETPAEIVSRIADGYALDASTMATNLGDARA
                     ANTVLLGVLSVSLDYAVSDWLDIIDGLVPAKSRLVNREAFEAGRTWALAAQADPSLRY
                     DETIRRYEAPGRQADVAITLEINEAWCKGCDICVKLCPERCLHLNAHQVAVLRQPEAC
                     TGCHVCEWLCPDFAITVRRQPNSAEVA"
     gene            12368..>13271
                     /locus_tag="PLQ11_05450"
     CDS             12368..>13271
                     /locus_tag="PLQ11_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HRK24380.1"
                     /translation="MTGALKSLHGNQACALGAIAAGCTFFAGYPITPSSEVAEHMARE
                     LPRVDGVFVQMEDEIGSLAAVIGASLGGVKAMTATSGPGYSLMQENLGYAAMTEAPCV
                     IINVMRGGPSTGMPTRPSQGDIMQARWGTHGDHPIVVVAPASVQEIYTETIRAFNLAE
                     QFRVPVVVLYDEIIAHLLETVDLPAKKDVVRIERVWASGNEPGFLPYADTPDHVPVMA
                     RPGDGHRIHVTGLTHLPDGFPTQKPELAERALCRLLDKIEVNLARIEHTEMLGTEDAD
                     VLVVAVGITARAARSAVQRLRAEGV"
BASE COUNT         2157 a         4289 c         4252 g         2573 t
ORIGIN      
        1 gaacgcgcgc tcgccgccat ccttgccgat atggcgaccg gcgagcgcat gctgcgactg
       61 cttcagggcg atgtcggctc cggcaagacg atcgttgcgc tgctggcgat ggcccgggcg
      121 gtcgaggccg ggcggcaggc ggcgctgatg gccccgaccg aaattctcgc ccgccagcat
      181 ttcgatcgcc tcgccccgct cgcggctgcg gccggcatgc ggatggcgct gctgaccggg
      241 cgtgcgaagg cgtccgaacg cgccgccgtt cttgccggcc ttgccgctgg cgaaatcgac
      301 cttgtcgtcg gcacgcatgc gctgtttcag gatgatgtgg cgttccgcga tctggcgctg
      361 gcggtggtcg acgagcagca ccgtttcggc gtgcaccagc ggctggcgct gagccgcaag
      421 ggcgatgcga ccgaccttct ggtgatgacg gccacgccga ttccgcgctc gctggtgctg
      481 acctatttcg gcgacatgga cgtgtcgatc ctcgatgaaa agcccgccgg gcgcctgccg
      541 atcgacacgc ggctgatcga tcttgaccgg ctcggcgagg tggccgaggg gatcggcagg
      601 gcgattgcca gcggcgcgcg ggtctactgg gtctgcccgc tggtggcgga aagcgaaacc
      661 tccgaccttg ccgccgccga agcccggcgc gacgatcttg cccggctttt gggcgatcgc
      721 gtcgggctcg tccatggccg gatgaagggc accgacaagg atgaggcggt gcgccgcttc
      781 cagtcgggag agactgcggt tctggtcgcc acgacggtga tcgaagtcgg cgtcgatgtg
      841 ccggaagcga cgatcatggt gatcgagcat gccgagcgct tcggcctcgc ccagttgcac
      901 cagttgcgcg gccgcgtcgg gcgcggcgcc ggcagatcga cctgccttct gctctacaag
      961 gccccgcttg gcgaggtggc cggggcgcgg ctgaaaattc tgcgcgagag cgaggacggc
     1021 ttccgcatcg ccgaggagga tctccgcctg cgcggcgaag gcgatgtgct cggcactcgc
     1081 cagagcggct cgcccggctt ccgcatcgcc cggctggagg cacatggcga tctcttgcgc
     1141 atggcccggc aggacgcgga actcgccctc aatcgcgacc cggacctgac cggtccgcgc
     1201 ggggcagccc tgcggctgct gctctacctg ttcgagcgcg aggaggcggt gcgactgttc
     1261 cgcgccggat gatgccggcg acaggactgg tttgggttgc aaatcgccgc gttccgttgt
     1321 ccattcactc caggaccgtc gtgcattttg catccagcgg cggcttcagg gagaaagaca
     1381 tgcgtaaact gacagttctt ctggttgcgg ccgctgcggt ggcgatgacc ggcgccgccc
     1441 aggcgcagca ggcggaatgg aaagagatcc gcatcggcac cgagggcgcc tacccgccct
     1501 tcaacaatct caacgccaag aaggaactcg aaggcttcga gatcgattac gccaatgccc
     1561 tttgcgagaa gatgaaggtc aagtgcacct gggtggtgca ggactgggat ggcatcattc
     1621 ccgcactgct gtccaagaaa tacgatgtga tcgtcgccgg catgaacgcg accgaggaac
     1681 gggccaagcg cgtcgacttt tcgatggtct ataccaagac gccgatctcg atgatcgccg
     1741 ccaaatccgt gacctcgacc gatgtctcgc cggccgccct caagggcaag gcgatcggca
     1801 cgcagggttc gaccatccac gccaacttcc tcgaaaagca ctacaaggat tcgacccccc
     1861 gcctctatcc gacgcaggag gaggccaacc tcgatctgct caacggacgg cttgactatg
     1921 tggtggccga caagctggcg cttgaggatt tcatcaacgg caagggcaag gattgctgca
     1981 agatgatcgc cgatgtgacg cgcgatccgg cgatccatgg tcccggcgtc ggcatggcgg
     2041 tgcgcaagga ggacggcgca ctcaaggccc tgctcaacaa ggccatcgag gactcgatca
     2101 aggacggcac gcacaagaag atcgccgaca agtggttcaa gttcccgatc ctgtaacaat
     2161 ctgaacgaga ccgggcgcgt cgtaaccggc gcgccttttc gtcatccggc gtgccgcccc
     2221 ggctcccggc acctttcctg accagagccc cggcgccccg atggatcgct atatcacgct
     2281 gctgtcgttc ggcccggaag gctggggcgc cgcgctgttg tccggcgcgc tggtgacgat
     2341 ttccatcgcg ttggcgacct tgccgttcgg tctggcactg gggctggcgg tggctctggc
     2401 caaacgctcg cgccatgcct ggctgcgcgg accgggcacg ctctacacca cgattttccg
     2461 gggcgtgccg gaacttctga cgctctacat catctacttc ggcatccaga tgctggttca
     2521 gtcgctttgg acctggcttg ggtttgccgg caccatcgag atgagcccgt tcctggccgg
     2581 cgtgtttgcg ctgggcatcg ttctggcggc cttttcggcg gaggtatggc tgggcgcgct
     2641 caactcggtg cagcgcggcc agcgcgaggc agcggctgcg ctgggcctca cgtcgtggca
     2701 ggcattccgg cttgtggtgt tcccgcagct gttccgcgtt gccctgcccg gcctcggcaa
     2761 caactggatg gtgctgctca aggaaacctc gctggtctcg gtgatcacgc tccaggacac
     2821 gatgttcatc gccacgcgcg ccaatgtagt gaccaaggag ccgttcctgt tttttggcgt
     2881 cgcgatgctg atctacctgt ttttctcggc ggtttcggcg gcgggactgg cgaaggccga
     2941 agcccggacc aaccgcggtt tcgccgcccc gggagcgcgc tgatgctgtg gtgcgaatat
     3001 cccggcgccg tcctcggcat cgaggttctg cagaactacg gctgccgcat ggcctcgggc
     3061 ctcgggctga cactacagtt ggtggcgatc tcctttggca tcggattgat tgccgggctg
     3121 ggcctggcct tgctgcggct caatgcgccc cgccccctcg cctggctggt cggcgcctac
     3181 atgacggtgt ttcgcggcac gccactgctc ggccagctgt tcctgatcta ctatggcctc
     3241 ggctcgttcc ggctgttctg gcaggatgtg gggctgtggt ggctgtttcg cgatccgctc
     3301 tattgctgcc tgctcgcctt cacgctgaac accgccgcct atcaggccga gatctttcgt
     3361 ggggcgctga tcgccctgcc gcgcggccag cgcgaggcgg cgatggcgct cggcctgcat
     3421 ccggtcccga cctatctcaa ggtgctgctg ccgcaggcac tgatcacggc gctgcgcccg
     3481 ctgggcaatg agctgatcgt catgatcaag gccagttcac tggcctcgct ggtgacgctg
     3541 tttgatctga tgggcgtgac caagctggcc ttcgcccggt cgttcgatct gacgatctat
     3601 ctggttgcgg cgctgcttta tcttgttctg gtcgagatca tccggcgcct gtgggataat
     3661 ctggaacggc ggttgacccg ccatctggct ctggcatgac cgcctattcg atcaatccgt
     3721 ctgttgccgc tgtcgaggcg ccgccgatcc cctctgcgca ggcgtggaag cgcgactatg
     3781 acggcgcgcg cgggccgatg atcgatctga cccaggcagt gcccggcacg ccgccgcccg
     3841 ccgacgtgct ggaaaggctg gcgatggcgg ccggctcgat cgaagccacg cgctacgggc
     3901 cgatccttgg cgatagcgca ttgcgccagg cccatgccgg cgaaacctgg cgcctgtacg
     3961 gcggcgccat cgagccgcag catgtggcga tcacctgcgg ctgcaatcag gctttcgtgg
     4021 tcacgatgct ggcgctggct cgggccggtg acgcggtgat cctgccggcg ccgtggtatt
     4081 tcaatcacaa gatgacgctg gacatgctgg gcctcgagac ccgggtgctg gcctgccggc
     4141 cggaggcggg cttcgtgccg gatcccgatg cggccgagag gctgatcgac gggcgttgcc
     4201 gcgcgctcgt tctggtcagc cccaacaacc cgaccggcgc gatctatcca ccgcagctgc
     4261 tggcgcgctt tttcgatctc tgccggcggc accggctggc gctggttctg gacgaaacct
     4321 atcgcgattt cctgccctcg gatgccgcgc cgcacgggct gctcgcgcgc tccgactggc
     4381 cggatacgct gatccagctc tattcctttt ccaagtcgca tgcgctgccc ggtcatcggc
     4441 ttggtgcgat tgtagcggca ccggcgacca ttggcgaaat cgccaaggtt caggacagcc
     4501 tgcaaatctg cccggcccgg ccggcccagg gcgtggtggc ggattccatt gaaagccagc
     4561 gcggctggcg ggcgatgcgc cgcgatgaac tgatcgcccg cggaaccgcc ttccaggcgc
     4621 tgtttgccag gcagaaccgc tggcggatcg acagtctggg cgcgtatttt gcctttgttg
     4681 cccacccgtt caccgggctg gattcggtgg ctgcggcacg ccgcctggcg ctcgaagccg
     4741 gcgtactcgg cctgccgggc agctacttcg gtcccgggct tgatggccac ctgcggcttg
     4801 ccttcgccaa tgtcgagacc gccggccttg ccgacctgcc gcagcggctg tcagcaatgg
     4861 cttgaggccg gagcggccga ccggccaatc cgctccggca aatccgtcgc tacgctttcg
     4921 ggagagttgc ggccaccgaa ggccgcgcca cgattcccgc tgcccaggcc gccagatgcg
     4981 gccgggttgc ccgccagtcg aaatcggtaa agcggaaatc gaggtagccc atgagcgttg
     5041 ccgccgcgat cgttccgatg tcgaaatgcg ccgccagacg atgcgcgtag tcgcgttcga
     5101 gcacatcgag cgatgtggcg atcttgtgca tctgcccctc gatccaggcc ccccagcgca
     5161 gcggttcggg gcgcagcacc tgctcgtagc gcgccagcag acaggcatcg agcgcctcat
     5221 cgcacagcga ttgcagaacc agggctttcc agcgctctgc cccggcggga aacagcttgc
     5281 tgtcccccgc cctggcggcg agatactcga cgatgacccg gctgtcgtag atcgcctcgc
     5341 cggcctcggt caccagcgtc ggaatcttgc ctgcgggatt gtgtcggttg atcgccttgt
     5401 ccttctcgat cggactgacc ggcccggtga gcagcgtgat cgtgtcggcc agccccgtct
     5461 cgtgggcaac gatcaacacc ttgcgggcat agggcgaagc gggcgagtaa tagagttgca
     5521 tgcgggcaaa tccttgcggg gttcgaggct gccctagtta gagcaggttc gggcagagtg
     5581 acaacggcct ttgccggtgg cctgctccaa atgaccgaaa tcgatcccat catccagacc
     5641 ggcagaaatg gccggctgcc cacttgcggt gccgcctgcc gcaaaaacgg cattggcgcc
     5701 accaaacccg cacgcaacgc ttgccagcgg ttcgcgggtc aggcatacac cggccgggcg
     5761 ctggccgccc gtgcggagac gtggccgaga ggctgaaggc actcgtttgc taaatgagca
     5821 taccctgaaa ggggtatcga gggttcgaat ccctccgtct ccgccattga tttgctaagc
     5881 gattggacag gcttggccat tccttagatc acgccgcatt ttgatggaat catctaaatg
     5941 cggataacgt gatcggtatc aataagttag agcatgcctt ctgcgattgg ccggtatcca
     6001 cttaatcgcg acatgctcta gcgtcggaag gaacgtcctg tttccatcgg ggtttgcggc
     6061 gccagaccac tttccgtctg gaccggaccg gttgcgtttc tcgggccgtg caggtgattt
     6121 cccggacggt ttcggtttgc ctgttggttc catcaggtca cctgggatca ggtgtgtagg
     6181 tctcggtagg ctgcggctcc aggaaattct ggtaccactt ccacagtgga cgggccgtct
     6241 ccgctttata tgcggtgttg atcgtcagcg ccgtgttggg gatcgcgatg aaatccccct
     6301 tgctcgcagc ggtcacccga ctgccttgct gcgtgagcat gctggcgccg tattcggtgg
     6361 ccaccttggg atcggttgcc aacgtataga ttgtcgacaa gctgttcgcc ctgtcgagat
     6421 agatggccgc cggtccgaag ttgaacttgc cggcgaactg gatcgaatgg ggcgttatgc
     6481 ctccgaatgt cttcagcgaa tgctgggcat agccgggcga cttaacgccg tcataattgt
     6541 tgtaggccat ttgattccac atgtagcgaa ggggcgacat gctgatggca ccgctgtagt
     6601 tgacctccgc tccgcctacc gtgaagccga acagcacctc ggtcgcgccg atcgaggaat
     6661 tcatcaggcc gcgcgccctg cccttgccgt cgttccagtc tgcacccggc atcgcgccgc
     6721 tgcccagggg tgccggcaga cgcgccaccg ccagcgactt gtcccagccg agcgtcaccg
     6781 agacgggcag gtgcggcgcc gcccacgtga tgttctcgga tgttttcttc atattgtact
     6841 gggcgcgggc aaattcgttg ttgtgggtaa cggttgggcc gtcgcttgcc caggcatcgg
     6901 cgatgcccag accgatggcc gcccccgaaa cgcggtttag aacgtttccg acgcccttgg
     6961 ccgcttctgc cgttcccttg actgcggtgg agaaatcgcc agcgagctgg cccccggctg
     7021 cagagaagcg gaccggcagg gcgttcactc cctgctgtag caacgtgcct tcatactggc
     7081 gagtgagcca gttcaccggt cgctgggcgg tggtgcccac aaagctgccg aaacgagagc
     7141 cggccacatc gtccagcaag ccagccgtct tgctgacggc ccaggacgtg gcttggccgg
     7201 cagggctctg cgcaaccgtc cgtccgaact gcaccattgc ctgcctgcca ggctccatcg
     7261 ccaggtcgac caccagatcg cgggcgggat gatagagctt ggcgctggcc caacgcgtca
     7321 actcgacgcc aggggtcgcc aaccgctgga tcactccagt gggattggca acaacacggg
     7381 gaagaatttt cacgccctcg ccgacgagct gaggcacatt cgccaggcca tactgcggat
     7441 tgaatttcaa attcggtacg tagggcagat cgcctggggt gtagacgtat tttgcaccct
     7501 tgccgttttt ccagctgagc tcgaattcct tttgggctga ttcaatgact tcaccggctg
     7561 tgcgggattt cgtcgcagcg ttcccggcta cgtcatcggc tgcgccggaa acgtcatcgg
     7621 ccgtctgaac aacgtcatca acaccccgaa cagcgtcgtc agcgacctga acggcatcgt
     7681 cggccatcgc gactacatca tctgttgcat tgaccaaacc gtcggcggct ttgtccgccg
     7741 tcttggtgaa tggcgatttc gatattccaa aaaacgtatt ggtgccttcg acacgtttgt
     7801 cgatcagctt atcaaagcgt ccgagcagcg atcgttcctg ctgttcctga gccagttcct
     7861 tggcggtttt cttggtggct gtctcgacga cagtctcggt cgcctggttg ctagcgccac
     7921 ttccggccgc ggcgagatca tcgaccgtat ttccggccgc agcgacatca tcagcgccac
     7981 tgctgacgac cttggcagct ggggccttga acaatgctcc aagcgcccgc ccgccagccg
     8041 caccgagaac gccgaattcc acgtcccgaa caacgccatt ccagccgtag gtgtcgccgg
     8101 tattggtggc atcgtccagc gtgtgcaggc cgatcgtcgt accggtggtg atggcggtcg
     8161 aggccagcag tccggtcccg ccggttgcca cgccgacagc gacgacggcc gtcacctcga
     8221 ctccggtttg cgtccggccg acatgcttgt ctgcctccgc gatcttggcg acggctgcgt
     8281 tagcctgctg gctgttctcg gcgttaaggg cattttgccg ggcaagggtg gcaggatcag
     8341 aggagcgcat gaaggcaaga gacgtgagct tgtcgacctg cttgcgcgcg tccagcgtgc
     8401 tggtcactcc tggcagtgca tcggccagcc cgtctgagaa tgacccgatc cagccgtcat
     8461 actttccttc cacctcacgc atggccttgt cagccataaa ggccttggct tcgcccgggg
     8521 aggcaaagac ctcatttgga tcgacacctg cctccaaagc ctttctcgcc cattccaagc
     8581 gatcactgga cgtcttgaac atgtctcgcc cggtctttgc ccagggtttg agcgagccat
     8641 cggtattgat cgccccgttg cgggcgttcc aactgtcgtt ggctgcctgc tcggccttga
     8701 tcgaagcaag agtctggtcg atacgggcgc cgttttcctg cacctggcgc ttgaggctgt
     8761 tcagattgcc ctcgatccgt tcgaggttct tcaagatctg ccgggcctcc ttgtcggatt
     8821 tgaccgagcg ttcctctgct tccagcaggg cgtcttcggc gagcgcggcg gtgacttcgc
     8881 ctgcgagaat cttgtcctgc cagatcgcat atttgtgctg ggcgtcgccc gtttcgttga
     8941 acagctcctg agcctgccgc tcggtcagct tcggatccgc cttgcggacg agcgccatga
     9001 actcgtcgcg gcccatcgta tcggcacctt tgaggccagc ttccgtcatc ttgccgcgaa
     9061 cgtattccag cacatgctga cgacgggcat ggctgaggtc tgccttgtag aacgttaccg
     9121 gttcatgcct cggtgcaccg tccggcaggt tgatcgcctc gccaacagcg agatagtcgc
     9181 cgtccctgcc cttttgaccc ctctcgaaca ttgcctgctg aacgctggca tcaaaacgct
     9241 tgccgtcagt gctgcccggc acgaactgcg ggttgagacg gtaaagatcc ttgatgctca
     9301 caccgtagcg agccgctaca tcgtagagcg ataccccgct gctggaaatc acaatcggac
     9361 ctgacacctt gggctccgct cgttgttttt tacgtttttt ttcataattt tttataaaat
     9421 gcaggccttg tgtcagaaca gcggtatcag atcagaaagg ccgccgcggg caaccggcga
     9481 agagtctcct gtccgcatcg gcctgcacct ggcactagcc ttggtcaaaa gacgcaggcc
     9541 ttcgggggcg aaggttgatt gaggtcaaat acttgtgcgc tgcagatggt gcacggtccg
     9601 ccccttggcc tgagccgcaa aacgctcagc ctggcatcct gacgaggggg cggcatgaac
     9661 cagcaacgac gcatcgagcc cgatatcgtt accgacaccc tgctgccgct gatgggcaac
     9721 gaggccatcg cgcgcggcgc atgggaggcc ggcgtgaggg tggctgcggc ctatcccggc
     9781 acgccttcga ctgaaattct cgaaagcctc ggcacctacc ccgccagtga tgtcagcgcc
     9841 gaatgggcga ccaacgagaa agtcgcgctc gatgtggtga tcggcgcggc gctgtgcggc
     9901 gtgcgtgcgc tcaccgcgat gaagcatgtc ggcctcaatg tcgccgccga tgcgctgatg
     9961 tcgcaatcct atatcggctc gcatggcggg ctggtgctga tcgtctgcga tgatcccggc
    10021 atccattcct cgcagaacga gcaggacacg cgcctgttcg cccggctcgc caacgtgccg
    10081 gtgctggagc cgtcggacgc ccaggaagcc tatgatttca ccaagctggc gttcgagatt
    10141 tccgaacagt tcgatacccc ggtcatcgtc cgctcgacga cgcgcctgtc gcatacccgc
    10201 tcgatggtca gggtcggggt gcgcagcgag gttccgggca agggctttgt cgagacatcg
    10261 accaagaccg tcaccattcc tgccaacgcc cgccgcctgc atccgaaagt gctggagcgg
    10321 gaggcgcgcc tcgcccgcta tttcgagact tcgccgctga cccgccgcga agccggcgag
    10381 actcggttcg gcatcatcac cgcttcgacc gcctatctct atgtcaagga agtcgcgccg
    10441 catgcatcgg tcctcaagct cggcgcctcg catccgctgc ccgaagcggc gatccgcgcc
    10501 ttcgccgcat cggtcgaccg actgcttatc gtcgaggaac tcgaaccggc actggagaag
    10561 gacatccgcg caatgggcat tccggcggag ggcaaggcgt tcttcccgcg tcttggagaa
    10621 ctgtcgccgg aactggtcca ggcgggactg gcggctgccg ggctgatgga gccggcggcg
    10681 gcggtccgtt cgctcaacat cgagccgatg gcgcggccgc cggttctgtg ccccggttgt
    10741 ccgcatacgg caacctacct tgccttgcgc ggcatcggag cccgggtttc cggcgacatc
    10801 ggctgctaca cccttgctgc ggtcgaaccg ctcaagtcga tggataccgc cgtctgcatg
    10861 ggcgccagca tcggcaatgc catcggcatg gccagatccg gggtcgatac caagccgatt
    10921 gtcgccacca tcggcgattc caccttcctg cattccggta tcccgccgct gatcgatgcg
    10981 gtctacaaca gggtcaacat cacggtggtg atcctcgaca atgcgattac cgcgatgacc
    11041 ggagggcagg atcaccccgg caccgggcgt tcgctgcgcg gcgagccgac ctacaaggtc
    11101 gattacgagc agatctgccg ggcggcgggc gtcgattggg tgcgcacggt ggatccctat
    11161 caggtcggcc acatgtatca gaccttgcgc gaggcgatcg tccatcgcgg cgtgtcggtg
    11221 gtcatcgcct cgcggccctg cgtgcttgac ccggtcaaga tcaagggcac cccgctcgcc
    11281 atcaacccag ccggctgcgt cgcctgccag gcgtgcatga accttggctg cccctcgctg
    11341 tcctggaccg aggagatgca tgaggggcgg cacaaggtga tgatcgaggc ggcgagctgc
    11401 atcggctgca cgctctgcgc ccaggtctgc ccttccgact gcatccagcc ggtggcgcca
    11461 tgagccaacc cgccaggtcg gagcgggcca accaggcgcg caacatcatg atcgtcggcg
    11521 tcggcggtca gggcgtgctg atgatttcgc gggtgatggc catgatgggc catcgccagg
    11581 gccatcaggt caagcagagc gaggtgcacg gcatggccaa gcgcggcggc gtggtgttca
    11641 gccatgtccg tttcggcgag gcggtgtggt cgccgaccat tgccaagggc gaggcggatg
    11701 cgctgatcgc gctcgaatgg gccgagggcc tgcgctggct ggaccatctg cggccggaga
    11761 ccggcgtgtt catcgccgac acgcggcgga tcgtgccgcc cttcgcctgc cgcaaccgcc
    11821 gggctggcgc tgccaccaac tatgcgctgg agacgccggc agagatcgtc tcgcgcattg
    11881 ccgacggcta tgcgcttgat gcatccacca tggcgaccaa tctcggtgat gcccgtgcgg
    11941 ccaataccgt tctgctcggc gtgctgtcgg tatcgctcga ttacgcggtg agcgactggc
    12001 tcgacatcat cgacgggctg gtgccggcaa agtcgcgtct ggtgaaccgc gaggcgttcg
    12061 aggccggacg cacctgggcg ctggcggcgc aggccgatcc cagcctgcgc tatgacgaga
    12121 cgatccgacg ctacgaggcg ccgggccggc aggccgacgt cgccatcacg ctcgaaatca
    12181 acgaagcctg gtgcaagggc tgcgacatct gcgtcaagct ctgcccggaa cgctgcctcc
    12241 atctcaatgc ccatcaggtg gcggtgctgc gacagcctga agcctgcacc ggctgtcatg
    12301 tctgcgaatg gctgtgcccg gatttcgcca tcaccgttcg caggcaaccc aattcggcgg
    12361 aggtcgcatg accggcgcgc tcaaatcgct ccatggcaat caggcctgcg cgctgggcgc
    12421 gatcgccgcc ggctgcacgt tctttgcggg ctatccgatc accccgtcat cggaggtcgc
    12481 cgaacatatg gcgcgcgaac tgccgcgcgt cgacggcgtg ttcgtccaga tggaggacga
    12541 gatcggttcg ctcgccgcag tgatcggcgc ctcgctcggc ggggtcaagg cgatgaccgc
    12601 cacctccggc cccggctatt cgctgatgca ggaaaacctc ggctatgcgg cgatgaccga
    12661 agcgccctgc gtgatcatca atgtgatgcg cggcggtccc tccaccggca tgccgacccg
    12721 cccctcgcag ggcgacatca tgcaggcgcg ctggggcacg catggcgacc atccgatcgt
    12781 cgtcgtcgcc cccgcctcgg tgcaggagat ctataccgag accatccgcg ccttcaatct
    12841 ggccgagcag ttccgcgtgc ctgtggtcgt gctctatgac gagatcattg cccatctgct
    12901 cgaaacggtc gacctgccgg cgaaaaagga tgttgtgcgg atcgagcggg tctgggccag
    12961 cggcaacgag cccggctttc ttccctatgc cgacacgccc gaccatgttc cggtgatggc
    13021 gcggccgggc gacggccacc gcatccacgt caccgggctg acgcatctgc cggacgggtt
    13081 tccgacccag aaaccggagc ttgcggaaag ggccctctgc cgcctgctcg acaagatcga
    13141 ggtcaacctt gcccgcatcg agcacaccga gatgctcggc accgaggatg ccgatgtgct
    13201 ggtggtggcg gtcggcatca ccgccagggc ggcccgcagc gcggtgcagc ggctgcgcgc
    13261 cgagggcgtg c
//
LOCUS       DAOMXG010000055        13252 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_2567_length_13252_cov_9.419868, whole genome shotgun sequence.
ACCESSION   DAOMXG010000055 DAOMXG010000000
VERSION     DAOMXG010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 13252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13252
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2567_length_13252_cov_9.419868"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..823
                     /locus_tag="PLQ11_05455"
     CDS             74..823
                     /locus_tag="PLQ11_05455"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HRK24381.1"
                     /translation="MPFPPIRLGVNIDHVATVRNARGGLLPDPVRAAHVAIEAGADGI
                     TAHLREDRRHIRDADIERLKAEITKPLNFEMAATEEMVAIALKLRPHACCLVPEKREE
                     RTTEGGLDVVGGKAALRPTIRRLKDAGIRVSLFIEPDRAAIETSAELGADIVELHTGT
                     WCEAAIHHDAGSDQELRRLIEGAAIGQAAGLEMHAGHGLDYRTAEKLAHCHPFVEFNI
                     GHFIVGEAIFVGFGEAVRQMKAAMIRGRKFR"
     gene            820..1221
                     /gene="acpS"
                     /locus_tag="PLQ11_05460"
     CDS             820..1221
                     /gene="acpS"
                     /locus_tag="PLQ11_05460"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011424723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HRK24382.1"
                     /translation="MILGLGSDLIDIRRIERSLQRFGSRFTGRVFTEAERLRSERKAA
                     RAASYAKRFAAKEACAKALGTGIRSGVFWSDMGVVNRPSGQPTMQLTGGALQRLRAMT
                     PAGMEAVVHLTMTDDFPLAQAFVVIEARPQG"
     gene            1307..2083
                     /gene="lepB"
                     /locus_tag="PLQ11_05465"
     CDS             1307..2083
                     /gene="lepB"
                     /locus_tag="PLQ11_05465"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRK24383.1"
                     /translation="MTDTPPRTAPPARKSEGGFGETLRVILEALAIALVVRTFLFQPF
                     NIPSGSMIPTLLIGDYLFVSKYSYGYSKHSFPFSLMPVGGRFLFVSEPKRGDVVVFKL
                     PKDNSTDYIKRLVGLPGDRLQVIRGVLFINGSAVKLERAGSFVTPDRWGRPAPTPLIR
                     ETLPGGFTHDIIEVEGDNAYLDNTSEYVVPAGHYFMMGDNRDNSQDSRVLSEVGFVPF
                     ENLVGRAEIIFFSMDENARFYEFWKWPWSIRWDRMFSGIR"
     gene            2083..2787
                     /gene="rnc"
                     /locus_tag="PLQ11_05470"
     CDS             2083..2787
                     /gene="rnc"
                     /locus_tag="PLQ11_05470"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HRK24384.1"
                     /translation="MAGDDRLAGLEARLGHRFADRALLEQALTHASALNGDEAGRASY
                     QRLEFLGDRVLGLAVAAMLFDRFPSAPEGQLSRALADLVRKETCAAVAIKLDLGPCLV
                     LGRGERRTGLAKKLSVLGDACEALLGAVYRDAGFAAASALVHRLWQDRLPELGSVATA
                     DPKTALQEWAHSQGLAEPRYEERGRSGPDHAPEFCIAVLVAGLEPVEASGKSKRAAER
                     AAAQRMLARQLGDVDG"
     gene            2780..3682
                     /gene="era"
                     /locus_tag="PLQ11_05475"
     CDS             2780..3682
                     /gene="era"
                     /locus_tag="PLQ11_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HRK24385.1"
                     /translation="MAEATRCGFVALIGAPNAGKSTLLNALVGAKVSIVSRKVQTTRT
                     QVRGIALTGQTQIIFVDTPGIFAPKRRLDRAMVTSAWGGAGDADLVCLLVDVHKAGDE
                     ANLALLDKVKELRAPVALVLNKIDTLPRERLLAIAADLNKDHAFAATFMVSALTGDGV
                     ADLMAWLAPRLPPGPWLYPEDQMSDAPLRFLAAEITREKLFERLHDELPYRSTVETEQ
                     WKTLRDGSVRIEQVIFVEREGHRKIVLGEKGRTIKAIGEAARGEIAEAAEAKVHLFLF
                     VKVRENWADDPERYREMGLEFPKG"
     gene            3737..4450
                     /gene="recO"
                     /locus_tag="PLQ11_05480"
     CDS             3737..4450
                     /gene="recO"
                     /locus_tag="PLQ11_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HRK24386.1"
                     /translation="MDWHDDGIIIGLRRHGETSVIVEAMTRSHGRHLGLVRGGRGRRH
                     APVLQPGNTVDLVWRARLDEHLGQYQVEPLVMRSRFLDSAAALYALSVVSGHLRLLPE
                     RDPHEALYEAATVIFDHADDPATACALIARFERALLGDLGFGLDLDACAATGAAADLI
                     YVSPKSGRAVSRDAGAPYADRLLALPDFLRNGGTSPMGADLAAAFRLTGHFLERHVFE
                     PRGLSLPDQRAALLALAVV"
     gene            4502..4969
                     /locus_tag="PLQ11_05485"
     CDS             4502..4969
                     /locus_tag="PLQ11_05485"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRK24387.1"
                     /translation="MSSVPIVRPETEAVPQFDLANVRTLRMVAFVIDAAVVVMLWWLC
                     GALMVALAWPTYGASFGFAPLMSLVGVFYAGLTMSGRGMGTWGMRAVGLKATDLGGQR
                     LGFIIAAGHALIFWLATTFSMGQLTIALLFVSLLNAAKRTAHDFLTGVIVSRR"
     gene            5042..5767
                     /locus_tag="PLQ11_05490"
     CDS             5042..5767
                     /locus_tag="PLQ11_05490"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HRK24388.1"
                     /translation="MTEQPRDAPQFYMTAPTTCPYLPGRMERKVFTHVVGRRAPELND
                     MLTHGGFRRSQTIAYRPACETCKSCVSVRVLTDEFRPSRGMRRVVRDNGDLSADMRPA
                     KATPEQYSLFRDYLDARHADGGMADMSALDYAMMVEDSHIDTRVVEYRLRCGPRAGVL
                     VGVALTDVLSDGLSMVYSFYDPSEDGRSFGTFMILDHIARARSLGLPFVYLGYWVDGS
                     PKMAYKARFGPQEQLTPLGWRRA"
     gene            complement(5779..6522)
                     /locus_tag="PLQ11_05495"
     CDS             complement(5779..6522)
                     /locus_tag="PLQ11_05495"
                     /inference="COORDINATES: protein motif:HMM:NF017655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HRK24389.1"
                     /translation="MTRPDLDAYARSLNGGPLDLMKTIAVFTMVLESANFVLFDNRYG
                     LPWYIDRVTFPIFCFASAVHIARGFDFRGQVATLAVLGVVAQPFHALAFPGYGGANVL
                     VTLAIGAALAGSLASRPPWQGDAVIALVAALNIALPAAISGGWEYGMLGAVLPLAFAS
                     GLKHGGIRLLWPVVVLTVMYAMNDFAKPEALRIPGLDIAFAVVGTALVILAGQMMVGR
                     PRFLHRYALHVVYPGHLAVLVGLRLLMGG"
     gene            complement(6618..6779)
                     /locus_tag="PLQ11_05500"
     CDS             complement(6618..6779)
                     /locus_tag="PLQ11_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24390.1"
                     /translation="MKKALACVALSLLIGGSGVLVSATPADAIGIVYGKKDGKQKKPA
                     KQQKAPQRR"
     gene            complement(6903..7079)
                     /locus_tag="PLQ11_05505"
     CDS             complement(6903..7079)
                     /locus_tag="PLQ11_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24391.1"
                     /translation="MKKVLAPLALALLVGGSGVLVSATPADAIGINWDGPRSKQKPAQ
                     KQQKQQPPRTKQRR"
     gene            complement(7195..7347)
                     /locus_tag="PLQ11_05510"
     CDS             complement(7195..7347)
                     /locus_tag="PLQ11_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24392.1"
                     /translation="MKKILMCSMLTLALAGGSFLASATPADAIGINFDGSKKKSKKPP
                     KAQQRR"
     gene            complement(7582..7941)
                     /locus_tag="PLQ11_05515"
     CDS             complement(7582..7941)
                     /locus_tag="PLQ11_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24393.1"
                     /translation="MKVFSALSGSLIVAFGVYAYWGEQPCTRIPPRHWLSPLEVEIRL
                     RDAQLRMEAVRTTSNRCYETVARDVYGMRHIMVIDPADGSILSSERKPASIADNQGNP
                     AAATGADGRRATPQVLD"
     gene            complement(8034..10277)
                     /gene="parC"
                     /locus_tag="PLQ11_05520"
     CDS             complement(8034..10277)
                     /gene="parC"
                     /locus_tag="PLQ11_05520"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HRK24394.1"
                     /translation="MGKPVEPPKDDEVEHVELKTALEERYLAYALSTIMHRALPDARD
                     GLKPVHRRLMHAMRLLKLDPGQGFKKCARVVGDVIGKYHPHGDQAVYDAMVRLAQDFA
                     QRYPLVEGQGNFGNIDGDGAAAYRYTEARLTDVAALLLDGIDEDAVDFRPTYNGEDEE
                     PIVLPAAFPNLLANGSQGIAVGMATSIPPHNAAELCQAALHLIDRPEATTDDLLQFVP
                     GPDFPTGGIIVEGRGAIAETYRTGRGGFRVRARWSREDGQRGTWVIVVTEIPYLVQKG
                     RLIEKIAELINDKKLPLLADIRDESAEDVRIVLEPRAKTVDPALLMESLFRMTELESR
                     LSMNMNVLVDGLAPRVIGLKEALKVWLDHRRVVLQRRSKYRLGKIEHRLEILGGLLIV
                     YLDLDEVIRIIREEDEPKPALMKRFDLTELQANAILDTRLRSLRKLEEMELKREHAEL
                     TAEKAQIEALLGSDAKQWKTIAWEIRQVLKKFGPETALGKRRTTFADAPEIGAIDITD
                     ALVEKEPITIVISEKGWIRALKGHVGDLSNVAFKGDDGLKLAFRGQTTDKLLVLATNG
                     KVFTLEAAKLPGGRGFGDPIRLMADIDPDADIVTVWPHEPGAKRLIIGRDARGFVVAS
                     DDLVANTRKGKNVLTVDSARAWLSVPVEGDGVAIIGENRKLLVFPLDQIPEMARGKGV
                     RLQRYKEGGIADAKTFSLAEGLTFTDSAGRTFTVKGKDLDEWRGNRAEAGKLPPKGFP
                     KTNRFVG"
     gene            10469..11752
                     /gene="glyA"
                     /locus_tag="PLQ11_05525"
     CDS             10469..11752
                     /gene="glyA"
                     /locus_tag="PLQ11_05525"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016553500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HRK24395.1"
                     /translation="MSAPRSNSFFSAPLAARDPEIARSIELELGRQRDEIELIASENI
                     VSRAVLEAQGSVMTNKYAEGYPGKRYYGGCQFVDIAEKLAIERACRLFDCKFANVQPN
                     SGSQMNQAVFLALLKPGDTFMGLDLNSGGHLTHGSPVNMSGKWFNVVPYGVRKDDELL
                     DYDAIAALALEHRPKLIIAGGTAYSRIWDFKRFREIADSVGAYLMVDMAHFSGLVAGG
                     AHPSPFPHAHVATTTTHKSLRGPRGGMILTNDEELAKKFNSAVFPGLQGGPLMHVIAA
                     KAVAFHEALQPEFKAYAHQVVKNAQALAKSMQDNGFRIVSGGTDNHLMLVDLRSRKVT
                     GKAAEAALGRAHITCNKNGIPFDPEKPMVTSGVRLGAPAATSRGFGVAEFTEVGNLIA
                     EVMDGLSKNGADGNGAVEEAVKAKVHALTARFPIY"
     gene            11865..12341
                     /gene="nrdR"
                     /locus_tag="PLQ11_05530"
     CDS             11865..12341
                     /gene="nrdR"
                     /locus_tag="PLQ11_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012169793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HRK24396.1"
                     /translation="MRCPYCGSLETQVKDSRPTDDNTSIRRRRVCPDCGGRFTTFERV
                     QLKELTVVKRSGRRVPFDRDKLARSIEIATRKRPVEPERIERMINGIVRQLEASSDGD
                     VTTEQIGEAVMEGLKGLDDIAYVRFASVYRNFREAREFGAFIDALEQGERPKEDGE"
     gene            12338..>13252
                     /gene="ribD"
                     /locus_tag="PLQ11_05535"
     CDS             12338..>13252
                     /gene="ribD"
                     /locus_tag="PLQ11_05535"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HRK24397.1"
                     /translation="MSTPAATDARFMAAALAYAAHGLGLTRPNPTVAALIVRDGIVIA
                     RGRTGLGGRPHGERLAIERAGSAARGATMYVTLEPCARRSGAGDALSCSEAIMAAGLA
                     RVVIAADDPSPFAAGAGSARLIEAGIAVTRGVLAEEARRLNLGHVLRIERHRPLVSLK
                     LARTADGFAGTLAGGPIMITGPVSFRHTHVARAEADALMVGVGTVLGDDPKLNCRLAG
                     LEHRSPLRVVVDPALRTPVASYLVRTAQRQPTRIICTETAPEARAEPLRASGVDLLRI
                     PAGVDGRPDLGLALAALAASGITRLMVEG"
BASE COUNT         2197 a         4311 c         4339 g         2405 t
ORIGIN      
        1 tagagcatgt cttctgcgat taaccggtat ccacttaatc gcgacatgct ctggcgacga
       61 aaggacagtg ccaatgccat ttccgccgat ccgcctcgga gtgaacatcg accacgtcgc
      121 aacggtgcgc aatgcccgcg gcggcctgct gcccgatccg gtgcgcgccg cccatgtcgc
      181 catcgaggca ggggcggacg gaatcaccgc ccatctgcgc gaggatcgtc gccatatccg
      241 cgatgccgat atcgagcgat tgaaggcgga gatcaccaag ccgctgaatt tcgaaatggc
      301 agccaccgag gagatggtgg cgatcgcgtt gaaactgcgc ccgcatgcct gttgtctggt
      361 tccggaaaag cgcgaggagc gcaccaccga gggcgggctt gatgtggtgg gcggcaaggc
      421 ggcgttgcgc ccgaccatcc ggcggctgaa ggatgccggg atccgcgtct cgctgttcat
      481 cgagccggac cgggccgcca tcgagacatc ggctgaactc ggcgcggata tcgtcgaact
      541 acacaccgga acctggtgcg aggcggcgat ccaccacgac gccgggtcgg accaggagct
      601 gcggcgcctg atcgagggtg ccgccatcgg tcaggctgcg gggctggaaa tgcatgccgg
      661 ccacgggctc gactaccgca cggccgagaa gctggcccat tgccatccgt tcgtcgaatt
      721 caacatcggc catttcatcg tcggcgaggc gattttcgtc ggttttggcg aggcggtgcg
      781 gcagatgaag gcggcgatga tccgggggcg caagttccgg tgattctcgg tctgggatcc
      841 gatctcatcg acatccgccg gattgagcgt tcgctgcaac gattcggcag ccgctttacc
      901 gggcgcgtgt tcaccgaggc cgagcgtctg cgctccgagc ggaaggcagc gcgcgccgcc
      961 tcctatgcca agcgctttgc cgccaaggag gcctgtgcca aggcattggg caccggcatc
     1021 cgttccggcg tgttctggag cgacatgggc gttgtcaacc gcccatccgg ccagccgacc
     1081 atgcaattga ccggcggcgc gctgcaacgg ctcagggcga tgacgcccgc cggcatggag
     1141 gctgtcgtcc acctgacgat gaccgatgat tttccgctgg cgcaggcctt tgtggtgatc
     1201 gaggcccgcc ctcagggctg atctgcacat tgctcctgtg cccaactgcg actataccgg
     1261 ctcgcatgag acgcccccag ggcgccgccc gaatggaacc ccgcgcgtga ctgacactcc
     1321 ccccaggaca gccccccccg cccgcaagtc cgaaggcggt tttggcgaga ccttgcgcgt
     1381 catccttgag gcgctggcga tcgcgctggt ggtgcgcacg ttcctgttcc agccgttcaa
     1441 catcccgtcc ggctcgatga tcccgacgct gctgatcggc gactatctgt tcgtttcgaa
     1501 atattcctac ggctactcga agcattcgtt cccgttcagc ctgatgccgg tcggcgggcg
     1561 tttcctgttc gtgtccgagc ccaagcgcgg cgatgtcgtg gtgttcaagc tgcccaagga
     1621 caattcgacc gattatatca agcgccttgt cggcctgccg ggcgaccggc ttcaggtgat
     1681 ccgcggcgtg ctgttcatca acggctcggc ggtcaagctt gagcgcgccg gcagtttcgt
     1741 cacgcccgat cgctggggcc gcccggcgcc gacgccactc atccgcgaga cgctgcccgg
     1801 cggcttcacc catgacatca tcgaggtgga aggcgacaat gcctatctcg acaacaccag
     1861 cgaatatgtc gttcccgccg gccattactt catgatgggc gacaaccgcg acaattcgca
     1921 ggacagccgg gttctgagcg aggtcggatt tgtcccgttt gagaatctgg ttggccgtgc
     1981 ggagatcatc ttcttctcga tggatgagaa cgcgcgcttc tacgaattct ggaaatggcc
     2041 ctggtcgatc cgttgggatc gcatgttcag cggcatccgc tgatggccgg ggacgaccgg
     2101 cttgccgggc ttgaggcgcg gctgggccac cggtttgccg atcgtgcgct gctggaacag
     2161 gcattgaccc acgccagcgc gctcaatggc gacgaggcgg ggcgcgccag ctatcagcgg
     2221 ctggaatttc tcggtgaccg ggttctgggg cttgctgtgg cggccatgct gttcgaccgt
     2281 tttccgagcg cgcccgaagg ccagttgtcg cgggcgctgg ccgatctggt gcgcaaggaa
     2341 acctgcgccg ccgttgcgat caagcttgat ctcggcccct gcctcgtcct ggggcgagga
     2401 gagcgtcgca ccggtctcgc aaagaagctg tccgtgctgg gcgacgcctg cgaggcgttg
     2461 cttggcgcgg tctatcgcga tgccggcttt gcggcagcgt ccgcgctggt gcatcggctg
     2521 tggcaggatc gcctgccgga actgggcagc gtggccaccg ccgatcccaa gacagcgctg
     2581 caggaatggg cgcacagcca ggggctggcc gagccgcgct atgaggagcg cggccgttcc
     2641 ggtcccgatc acgcgcccga gttctgtatc gcagtgctgg tcgccggact tgagccggtt
     2701 gaggcgtccg gcaagtccaa gcgtgcggcc gagcgtgccg cagcccagcg catgcttgcc
     2761 cgccagttgg gagacgtcga tggctgaagc aacccgctgc ggttttgtcg cgctgatcgg
     2821 cgcgcccaat gccggcaagt cgacgctgct caatgcgctg gtcggtgcca aggtgtcgat
     2881 cgtctcgcgc aaggtgcaga ccacccgcac ccaggtgcgc ggtatcgcgc tcacagggca
     2941 gacgcagatc atctttgtcg atacgccggg cattttcgcc cccaaacgcc ggctcgaccg
     3001 ggcgatggtc acctccgcct ggggcggggc aggggatgcc gatctcgtct gcttgctggt
     3061 ggatgtccac aaggccggcg acgaggccaa tctggctttg cttgacaagg tgaaggagtt
     3121 gagggcgccg gtggcgctgg tgctcaacaa gatcgacacc ctgccgcgcg agcggctgct
     3181 ggcgattgcc gccgacctca acaaggacca tgccttcgcc gcaaccttca tggtctcggc
     3241 gctgaccggc gatggcgtcg ccgatctgat ggcctggctg gcgccgcgcc tgccgccggg
     3301 gccgtggctc tatcccgagg atcagatgtc ggatgcgccg ttgcgcttcc ttgccgccga
     3361 aatcacccgc gaaaaactgt tcgaacgcct gcacgacgaa ttgccctacc gctcaaccgt
     3421 cgagaccgag cagtggaaga ccctgcgcga cggctcggta cgcatcgagc aggtcatttt
     3481 tgttgagcgc gagggccatc gcaagatcgt gctgggcgag aagggccgca cgatcaaggc
     3541 catcggcgag gcagcacgcg gcgagattgc cgaggcggcg gaggccaagg tgcacctgtt
     3601 cctgttcgtc aaggtgcgcg agaactgggc cgacgatccc gagcgctacc gcgagatggg
     3661 gctggagttt ccgaaagggt agtgcggccc ggcaaggcgt gctctgctgc gccgatcgcg
     3721 caatagattc gcccgcatgg actggcacga cgacggcatc atcatcggct tgaggcgcca
     3781 cggcgagacg tcggtcatcg tcgaggcgat gacgcgcagc catggccgcc atcttgggct
     3841 ggtgcgcggc ggacgcgggc gtcgccatgc gccggtgctg caacccggca acacggtcga
     3901 tcttgtctgg cgtgcgcggc tggatgagca cctcggccag tatcaggtcg agccattggt
     3961 gatgcgttcg cgctttctcg acagtgcggc ggcgctttac gcgctgtcgg tggtgtccgg
     4021 tcacctgcgg ctgttgccgg agcgcgatcc gcacgaagcg ctctacgagg cggcgacggt
     4081 gattttcgac catgccgacg atccggccac cgcctgcgcg ctgatcgccc ggttcgaacg
     4141 ggcactgctg ggcgatcttg gcttcggcct cgatctcgac gcctgtgccg caaccggggc
     4201 cgccgctgat ctcatctatg tctcgccgaa atcggggcgc gcggtcagcc gcgatgccgg
     4261 cgcaccctat gccgaccggt tgctggcttt gccggatttc ctgcgcaatg gcggaacctc
     4321 gccgatgggc gcggaccttg cggcggcctt tcggctcacc ggccattttc tggagcggca
     4381 tgtgttcgag ccgcgcggcc tcagcctgcc cgaccagcgc gccgcgctgc tggctctggc
     4441 cgttgtttaa agaaggttca gacgcgccta tataggaagc gccgtcctgg ggagcctgcc
     4501 tatgtcgtct gtgccgattg tccgcccgga aaccgaagct gtgccgcagt tcgatcttgc
     4561 caatgtgcgg acgctgcgca tggtcgcctt cgtgatcgat gccgccgttg tggtgatgct
     4621 gtggtggctg tgcggcgcgc tgatggtggc gctggcctgg ccgacctacg gcgcctcgtt
     4681 cggctttgcg ccgctgatgt cgctggtggg tgtattctac gccggcctca ccatgtccgg
     4741 gcgcggcatg ggcacctggg gcatgcgcgc ggtcgggctc aaggcaaccg atcttggtgg
     4801 tcagcgcctc ggcttcatca tcgccgccgg gcatgcgctg atcttctggc tggcgacgac
     4861 cttctcgatg gggcagctga ccatcgcgct gctgttcgtc tcgctgctca atgcggccaa
     4921 gcgcaccgcg catgatttcc tgaccggagt gatcgtgtcg cgccgctgag cccggccttg
     4981 cctcggggcg gatccggacg cactatggcc ggatcgattt ctgcccgtgc cgagtcttgt
     5041 cttgaccgaa caaccgcgcg acgccccgca attctacatg acggcgccga ccacctgccc
     5101 ctatctgccg gggcggatgg agcgcaaggt gttcacccat gtggtcgggc gccgcgcgcc
     5161 cgaactgaac gacatgctga cccatggcgg gttccgccgc agccagacca tcgcctaccg
     5221 gccggcctgc gagacctgca aatcctgtgt ctcggtgcgg gttctgaccg atgagttccg
     5281 gccttcgcgc gggatgcggc gcgttgtgcg cgacaacggc gacctcagcg ccgacatgcg
     5341 cccggcgaag gcaacgccgg agcagtattc gctgttccgc gattatctcg atgcccgcca
     5401 cgccgatggc ggcatggccg acatgtcggc gctcgattat gccatgatgg tcgaggacag
     5461 ccatatcgac acccgcgtcg tcgaataccg gttgcgttgc gggccgcgcg ccggcgtgct
     5521 ggtcggcgtg gcgctgaccg atgtgctcag cgacgggctg tcgatggtct attccttcta
     5581 cgatcccagc gaggacggcc gctcgttcgg caccttcatg atcctcgatc atatcgcccg
     5641 cgcccgcagc ctcggcctgc cgttcgtcta tctcggctac tgggtcgatg gctcgcccaa
     5701 gatggcctac aaggcccgct tcggcccgca ggaacaattg accccgcttg gctggcgccg
     5761 ggcgtagcgc cgacgggcct aacctcccat cagcagccgg aggccgacaa gcaccgccag
     5821 atggccggga tagaccacat gcagcgcata gcgatgcaga aacctcggac ggccgaccat
     5881 catctgtcct gccaggatga ccagcgccgt gccgacaacc gcaaaggcga tgtcgagccc
     5941 ggggatgcga agcgcttcgg gcttggcgaa gtcattcatc gcatacatca cggtcaggac
     6001 cacaaccggc cataacaggc ggatgccgcc atgtttgaga ccgctggcaa aggccagcgg
     6061 cagcaccgcg ccgagcatgc catattccca tccgccggaa atggcggcgg gcagggcaat
     6121 attgagcgcc gcgaccagag caatgaccgc atcgccctgc cagggtggcc ggctcgccag
     6181 cgagcccgcc agcgccgcgc cgatggccag cgtcaccagc acattggccc cgccatagcc
     6241 gggaaaggcc agcgcatgga acggttgcgc cacaacgccg agcaccgcca gcgtcgcaac
     6301 ctgcccgcgg aaatcgaacc cgcgcgcgat atgcaccgcc gaggcaaagc agaagatcgg
     6361 aaaggtgacc cgatcgatat accacggcag gccgtagcga ttgtcgaaca gcacgaaatt
     6421 cgcgctctcc agcaccatgg tgaaaacggc gatcgttttc atcaggtcga gcgggccgcc
     6481 gttcagcgag cgggcatagg cgtcaagatc aggccgtgtc atggcaagca ccaattctgc
     6541 acaatacccc cacgcccgcc ggccggccac cagtccggtg gtcggggcag gacgtttccg
     6601 acctcacgcc gaacgcgtca acgccgctgc ggcgccttct gctgtttcgc cggctttttc
     6661 tgcttgccgt ccttcttgcc gtagacgatg ccgatcgcat cggcaggggt ggccgagacc
     6721 agaacgcccg atccgccgat cagcagcgaa agtgcaacac aggcgagcgc ctttttcatc
     6781 gttggttcct ccggaaatat cagccgactg tcgcagcgac agatggcccg atccattcag
     6841 ccagggcacg ggcatctcaa gcgatgcccg cgcctgtcag cttgcggcgt tggccgctgg
     6901 cgtcagcgcc gctgcttggt ccttggcggc tgctgcttct gctgcttctg ggccggcttc
     6961 tgcttgctcc gcgggccatc ccagttgatg ccgatcgcat cggccggagt ggctgagacc
     7021 agaacgcccg atccgccgac cagcagggcg agggcaaggg gagcgagaac ttttttcatg
     7081 tgtcacctca gggaacgaac gccgcaacag gagcgccccg gcgtccaggt ccggttatat
     7141 caaatcagac gctttcggca tcaacgggcc ttgaggaccg tcgtacctca gcgatcagcg
     7201 ccgctgctgg gcttttggcg gcttcttgct tttcttcttg ctcccgtcaa agttgatgcc
     7261 gatggcatcg gccggcgtcg ccgaagccag gaagctgccg cctgcaagcg ccagcgtcag
     7321 catcgagcac atcaggattt tcttcatgcg aacctccgtt cagaccagcc gttgatcggc
     7381 tctggccatc gcgggtcagt gtggccctgc gtgccatcgt ccgccttgcg ctagatcatt
     7441 ggcgccgcat caatccgtca catgtcggag catcgcgtcg ggctttggca gggcgctgtc
     7501 gatcgcgcgg tgattcttat ggagttgcca aagcacggaa catgaaccgg attcaacctg
     7561 atcgcagcat gccctggaac gtcaatcaag gacctggggc gttgcccggc gaccatccgc
     7621 accggttgca gcagccggat taccctgatt gtcggcaatc gaggcgggtt tgcgctccga
     7681 ggacaggatc gatccatcgg cggggtcgat gaccatgatg tgccgcatgc catagacatc
     7741 gcgcgcgacc gtttcataac agcggttgga ggtggtccgc accgcttcca tgcgaagctg
     7801 tgcatcgcgc agcctgatct cgacttcgag cgggctgagc cagtgccgtg gcgggatccg
     7861 ggtgcagggc tgctcgcccc agtaggcgta aacgccgaat gcgacgatca atgagccgga
     7921 cagggcagaa aagaccttca tgaaaatctc ccggcgaagg ggccaggagc aatacaacct
     7981 aaaattgtta aaaatcaaac aatgcaactt ttgcgcttgt catggtgcgg ccgttacccc
     8041 acgaagcggt tggtcttggg aaagcccttg ggtggcagct tgccggcttc ggcgcggttg
     8101 ccgcgccatt cgtcgaggtc cttgcccttg acggtgaagg tgcgcccggc gctgtcggta
     8161 aaggtcaggc cctcggcgag gctgaaggtc ttggcatcgg cgatgccgcc ttccttgtag
     8221 cgttgcagcc gcacgccttt gccgcgtgcc atctccggaa tctgatcgag cgggaacacc
     8281 agcagcttgc ggttctcgcc gatgatcgcc accccgtcgc cctcgaccgg caccgacagc
     8341 caggcccgcg cgctgtcgac cgtcagcacg ttcttgccct tgcgcgtgtt ggcgacgaga
     8401 tcgtcggagg cgacgacaaa gccgcgcgca tcgcggccga tgatgaggcg ctttgccccc
     8461 ggttcatgcg gccagacggt gacgatgtcg gcatcgggat caatgtcggc catcagccgg
     8521 atcggatcgc cgaaaccgcg cccgccgggc agcttggcgg cttccagcgt gaacaccttg
     8581 ccgttggtgg cgagcaccag cagcttgtcg gtggtctggc cgcggaaggc aagcttgagc
     8641 ccgtcatcgc ccttgaacgc gacgttcgac aggtcgccga catggccctt gagcgcgcgg
     8701 atccagccct tttccgaaat gacgatggtg atcggttcct tctcgaccag cgcatcggtg
     8761 atgtcgatgg cgccgatctc gggcgcgtcg gcgaaggtcg tgcgccgctt gccgagcgcc
     8821 gtctcagggc cgaatttctt cagcacctgc cggatttccc aagcgatcgt cttccattgc
     8881 ttggcatcgg agccgagcag ggcctcgatc tgcgcctttt cggcggtcaa ttccgcgtgc
     8941 tcgcgcttga gttccatctc ctccagcttg cgcagcgagc gcagccgggt atcgagaatg
     9001 gcattggcct gcaattcggt gaggtcgaag cgcttcatca gcgccggctt cggctcatcc
     9061 tcctcgcgga tgatgcggat cacctcgtcg agatcgagat agacgatcag caggccgccc
     9121 aggatttcga ggcgatgctc gatcttgccg agccggtatt ttgaacgccg ctgcaacacg
     9181 acgcggcgat ggtcgagcca gaccttcagc gcttccttga ggccgatgac acgcggggcg
     9241 aggccatcga ccagcacgtt catgttcatc gacaggcgcg attccagttc cgtcatccgg
     9301 aacagcgatt ccatcagcag cgcgggatcg accgtcttgg cccgcggctc cagcacgatg
     9361 cgcacatcct cggccgattc atcgcggata tcggcgagca gcggcagctt cttgtcgttg
     9421 atcagttcgg cgatcttctc gatcagccga cctttctgga cgaggtaggg aatttcggtg
     9481 acgacgatca cccaggtgcc gcgctggccg tcctccctgc tccagcgggc ccgcacccgg
     9541 aaaccgccgc gcccggtccg gtaagtctcg gcgatcgcgc cgcgcccctc gacgatgatg
     9601 ccgccggtgg ggaaatccgg ccccggcacg aattgcagga gatcgtccgt cgtcgcctcg
     9661 ggcctgtcga tcagatgcag cgccgcctgg cacagttcgg cggcattatg cggcggaatc
     9721 gaggttgcca tgcccaccgc gatgccttgc gaaccgttgg cgagcaggtt ggggaatgcg
     9781 gcgggcagga cgatcggttc ctcgtcctca ccgttgtagg tggggcggaa atcgaccgca
     9841 tcctcgtcga tgccgtcgag cagcagcgcg gcgacatcgg tcagtcgcgc ctcggtatag
     9901 cggtaggcgg cggcaccatc accgtcgatg ttgccgaaat tgccctggcc ctcgaccagc
     9961 ggatagcgct gggcaaaatc ctgcgcgagg cggaccatcg cgtcatagac cgcctgatcg
    10021 ccatgcggat gatatttgcc gatgacatcg ccgacgacgc gggcgcattt cttgaagccc
    10081 tggccgggat caagcttgag caggcgcatg gcatgcatca gccggcgatg gaccggcttc
    10141 agcccgtcac gcgcatcggg cagggcgcga tgcatgatgg tcgacagcgc ataggcaaga
    10201 tagcgctctt ccagcgccgt cttgagttcg acgtgctcga cctcgtcgtc tttcggcggt
    10261 tcgacaggct tgcccatgcc cctcgctagc aggcgaggcg attcggggga aggcaccccg
    10321 cgcaaggagc gccatccggc aagaccggcg agggggcagg caaacggcag tcctgtagcc
    10381 gatgccgcag cccgcgctgc accctttccc cgctgcgccc cccatgctaa gcggcgggcg
    10441 cgtttttctc tgccgtgacg aggccctcat gtccgctccc cgttccaatt cgttcttttc
    10501 cgcgccgctt gccgcgcgtg atcccgagat cgcccggtcg atcgagctgg agctgggccg
    10561 ccagcgcgat gagattgaat tgattgcctc cgaaaacatt gtctcccgcg ccgtgctgga
    10621 agcgcagggc tcggtgatga ccaacaaata cgccgagggc tatccgggca aacgctacta
    10681 tggcggctgc cagttcgtgg atatcgccga gaagctggcc atcgaacgcg cctgcaggct
    10741 gttcgactgc aagtttgcca atgtccagcc caattccggc agccagatga accaggcggt
    10801 gtttctggcg ctcttgaagc cgggcgacac gttcatgggc ctcgatctca actccggcgg
    10861 gcatctgacg catggctcgc ccgtcaacat gtcgggcaag tggttcaatg ttgtccctta
    10921 tggcgtgcgc aaggacgacg aactgctcga ttacgatgcg atcgccgcgc tggcgctgga
    10981 gcaccggccg aagctgatca ttgccggcgg cacagcctat tcgcgcatct gggatttcaa
    11041 gcgcttccgc gagatcgccg acagcgtcgg cgcgtatctg atggtcgata tggcgcattt
    11101 ttcgggtctg gtcgccggcg gcgcccatcc ctcgccgttc ccgcatgcgc atgtcgccac
    11161 gacgacgacg cacaaatccc tgcgcgggcc ccgcggcggc atgatcctca ccaatgacga
    11221 ggaactggcc aagaagttca actccgcagt gtttccgggc cttcagggcg gcccgctgat
    11281 gcatgtcatc gccgccaagg cggtggcgtt ccacgaggca ctccagcccg agttcaaggc
    11341 ttacgcccat caggtggtca agaacgcgca ggcgctggcg aaatccatgc aggacaacgg
    11401 cttccgcatc gtctcgggcg gcaccgacaa tcacctgatg ctggtcgatc tcaggtcgcg
    11461 caaggtcacc ggcaaggcgg cggaagcggc gctcggccgc gctcatatca cctgcaacaa
    11521 gaacggcatt ccgttcgatc cggaaaagcc gatggtcacc tcgggcgtgc gcctcggcgc
    11581 cccggctgcg acctcgcgcg ggttcggcgt ggccgagttc accgaggtcg gcaacctgat
    11641 cgccgaggtg atggacgggt tgtcgaagaa cggcgcggac ggcaacggcg cggtcgagga
    11701 ggcggtgaaa gccaaggtcc acgcgctgac agcaagattc ccgatatatt gagggacgat
    11761 gtatcgcccg cccacgccgt ggtaagacag gcacaatccg gcgacgtgga cggccgggcc
    11821 aagcccgacg atgacgaacg gcctaccatc gctaggaaca tcccatgcgc tgcccctatt
    11881 gcggctcgct tgaaacgcag gtgaaggatt cccgcccgac ggatgacaac acgtcgatcc
    11941 gccgccggag agtctgcccc gattgcggcg gccgcttcac caccttcgag cgcgtgcagc
    12001 tcaaggaact gacggtggtc aagcgctccg gacggcgcgt gccgttcgat cgcgacaagc
    12061 tggcccgctc catcgagatc gccacgcgca agcgcccggt cgagccggag cgcatcgagc
    12121 ggatgatcaa cggcatcgtc cggcagctgg aggcatcctc cgacggggat gtcacgaccg
    12181 aacagatcgg cgaggcagtg atggaggggc tgaaggggct cgacgacatc gcttatgtgc
    12241 ggttcgcctc ggtctatcgc aatttccgcg aggcgcgcga attcggcgcc ttcatcgacg
    12301 cgctggaaca gggcgagcgc cccaaagagg acggcgagtg agcactccgg ccgccaccga
    12361 tgcgcggttc atggccgccg cgctcgccta tgcggcccat gggcttgggc tgacgcggcc
    12421 caaccccacc gttgccgcgc tgatcgtccg cgatggcatt gtcatcgccc gcggccgcac
    12481 gggcctaggc ggtcgcccgc atggcgagcg ccttgcgatc gagcgggccg gttctgctgc
    12541 gcgtggtgcc accatgtatg tgacgctgga gccgtgtgcc cgtcgctcgg gcgccggcga
    12601 tgcgctgtcg tgcagcgagg cgatcatggc tgccggactg gcccgcgtgg tgatcgcggc
    12661 ggatgatccc agcccctttg ccgctggtgc cgggtccgcg cggctcatcg aggccggaat
    12721 tgccgtcacc cgtggcgtgc tggcggagga ggcgcggcga ctcaatctcg gccatgtcct
    12781 gcgtatcgag cggcaccggc cgctggtcag cctcaagctg gcccggacgg cggatggatt
    12841 tgccggcacg ctggccggcg gaccgatcat gatcaccggt ccggtcagct tccgccacac
    12901 ccatgtcgcg cgcgcagaag ccgatgcgct gatggtcggt gtcggcacgg tgctgggcga
    12961 tgatcccaag ctcaattgcc ggctggcggg cctcgaacac cgttcgccgc tccgggtggt
    13021 ggtcgatccc gcgctgcgga cacctgtcgc ctcctatctg gtgcgcacgg cgcagcgcca
    13081 gccgacacgg atcatctgca ccgagacagc gcccgaagcg cgcgccgaac cgttgcgtgc
    13141 gtcgggagtc gatctgctgc gcattccggc aggcgtcgat ggccggcccg atcttggcct
    13201 ggcgctggcc gctcttgcgg cctccggcat cacgcggctg atggtcgagg gc
//
LOCUS       DAOMXG010000056        12920 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_2644_length_12920_cov_8.905014, whole genome shotgun sequence.
ACCESSION   DAOMXG010000056 DAOMXG010000000
VERSION     DAOMXG010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 12920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12920
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2644_length_12920_cov_8.905014"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1408
                     /gene="rpoD"
                     /locus_tag="PLQ11_05540"
     CDS             <1..1408
                     /gene="rpoD"
                     /locus_tag="PLQ11_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HRK24398.1"
                     /translation="APKVEPPADVFADPPKPVRSAGDDDDEAESRAPEPEPDNFDDEF
                     DGGVSLSAMEADLKPRVLETFGRIADNYKKLRRLAEANVENKLQSKSLSPAQERKYKK
                     LKEEIVADVKSLSLNQNRIDALVEQLYDINKRLIGLEGRLLRLAESYGVDRVDFLKHY
                     QGSELDSKWLLRISKFTSRGWKEFVAKDIDRIKEFRAEIQALSAETGLEVSEYRKIVL
                     MVQKGEREARQAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEY
                     RRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKIVRTSRQMLHEIGREPT
                     PEELSEKLAMPLEKVRKVLKIAKEPISLETPIGDEEDSHLGDFIPDPNVMLPIDAAIQ
                     SNLRETTTRVLASLTPREERVLRMRFGIGMNTDHTLEEVGQQFSVTRERIRQIEAKAL
                     RKLKHPSRSRKLRSFLDN"
     gene            1476..2003
                     /locus_tag="PLQ11_05545"
     CDS             1476..2003
                     /locus_tag="PLQ11_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24399.1"
                     /translation="MKRVLAVGFAMVWAASGGAHAEPLTPVYSSIQNCTPLPRLELPG
                     RTIVRTEATAAARCRGVGGFDLAVVEEDTRSWLAVIAPGGVHPLNRAMVQEFRHGHFP
                     TITDNKVVEWRVDGQGQPRALIVRVHFTDPDMPATSPAARKSLLMVFDLRALPPRLAG
                     TAPDNAGARRVADGE"
     gene            complement(2042..3481)
                     /locus_tag="PLQ11_05550"
     CDS             complement(2042..3481)
                     /locus_tag="PLQ11_05550"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRK24400.1"
                     /translation="MKLEESHERQPAFGEVQAPGRACDLARRLFGAQSALLVLHSAGV
                     SRVIASSGTRQPYRSRQLDFSIAPYARDAEVVQPDASGEPHYQPIATLLGLPRLGFFL
                     RTPVVVAAAYHLALLIGDPEPGKRPTPAKRKLLDNIKALIREEFSRHAPLLTDPSAGV
                     TAAITLDTASQLVADCTMPAALLKADQTILAANQPMASLLDRPLDSIVGRRHSELAVP
                     MGEAISALYRHAIESRISPPDCEVVTETESGRESFQLSISPFSPIETRDYFLFVTVAD
                     TASSNALEASITRQVESDMARPREPSLAFLDETLVHRRTIRARNGVHYLTLRSWRTSL
                     RDWQIKALRALKANTPSDMPSMIAEEMRYEMSALIGAQAFRAVVPIPCGHSGEGPCLS
                     LEIARALGAKLDIPVIQAFVTQPLRGKSHPKENLRRPPLALIRSFEGPLLLVDDVATS
                     GSHLEEAVKLLRPQSGSVFAVAWISGDAA"
     gene            3601..3876
                     /locus_tag="PLQ11_05555"
     CDS             3601..3876
                     /locus_tag="PLQ11_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002964584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyU family transcriptional regulator"
                     /protein_id="HRK24401.1"
                     /translation="MSFFAKLFGKSAAQAPEPVNEDYRSYILTATPYQEAGQWQMCGV
                     ISKEIGNETKQHRFVRADRFPSREDAVEFTLKKARQIVDLNGDRIFS"
     gene            4014..5231
                     /locus_tag="PLQ11_05560"
     CDS             4014..5231
                     /locus_tag="PLQ11_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24402.1"
                     /translation="MTDAILRAPPSSGQNPVYSLLFSRSFMGRPYLISPVADVLMAGG
                     GALFLLAVLSLTLPTQAGRAAPEAIATTAAIFATVSYFINYPHFMASYSLLYGSLGQR
                     MAAYSPGQMTWWRTVNAAFVAPAVLVAFFAYCVAWRRVDLMGWAVQAMFFFVGWHYVK
                     QSYGVFVVLSGMKGIYYNRWQVLALKGHVYSLWLYSWFSSGAVILEGMSPRPMNFHSI
                     SYDGLSLFKTQGLVDFLFWPTVFFALAGWAAVLWNWYATGKRPSLSGLLGYTSMYTLL
                     AFVRLHPLFAYAAPAFHSLQYLLFVYAYKRGEFHLEGQDHAVPDTGAKPPWLRLLTYF
                     LLMCATGALFFDVLPFYFDNKSTRWGLPLVATPIFHLFINVHHYFIDSAIWRRDNRDV
                     SRFLFWRAQAERR"
     gene            5293..6534
                     /locus_tag="PLQ11_05565"
     CDS             5293..6534
                     /locus_tag="PLQ11_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010686516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRK24403.1"
                     /translation="MQGSSSAPEHLPDSAYAWRRLAACVLVGTMASAGMWTVVVALPT
                     VQAEFGTARAGASLPYTALMIFFGVGTIIMGRAADRFGLTIPVIVAAVAIGLGYVISG
                     MATSLTMFTLAHGVLIGFGGGAGFAPLMSDISHWFVRRRALAVTLAACGSYLAGTLWP
                     LVMNLTMPLYGWRATHVGIGLAVMVIMLPLAFMFRVKPSQASLAASESATAAARADLG
                     IPPGRLQAMLTAAGLSCCIAMSMPQVHIVAYCGDLGYGVARGAEMLSLMLGLGIVSRI
                     ASGFLADRIGGPATLMIGSLMQGMALLLYLFFDGLSSLYLISAIFGLFQGGIVPMYAV
                     IAREFLPPREAGARIGIIISATIVGMAVGGYLSGLIFDLTSSYRMAFLHGLLWNFVNL
                     ALTGWLVARRRQGMALAGAAR"
     gene            6665..7057
                     /locus_tag="PLQ11_05570"
     CDS             6665..7057
                     /locus_tag="PLQ11_05570"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015951100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HRK24404.1"
                     /translation="MTPTPEPTDPRGDLTVRTIAMPADTNANGDIFGGWVMSRMDQAG
                     GIAGVRRARGRVVTVKVQAMTFHRPVRVGDVLTVYTRIGRIGRTSMDIEIEAWARRFL
                     TGEEEKVTDGVFTFVAITDEGKPRPIPR"
     gene            complement(7076..9829)
                     /gene="ppc"
                     /locus_tag="PLQ11_05575"
     CDS             complement(7076..9829)
                     /gene="ppc"
                     /locus_tag="PLQ11_05575"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HRK24405.1"
                     /translation="MTQRDQPRSADKDQSLIDDIRLLGQILGDTVREQEGADVFDLIE
                     TIRRLSVAISRDNSQSAAETLDMLLRAIPSDKAVSVVRAFTYFSHLANIAEDRHHIRR
                     REAHEAAGDRPQDGSLERCFDRIGDAGMAADTVRAMLETAWLSPVLTAHPTEVQRKSI
                     LDAERAIAHLLVDRENLRTARDRDRNLASLKARVAQLWQTRLLRTSKLSVEDEIDNAL
                     SYYRTTFLKEIPRLYGRLEELTGARVAPFLRMGSWIGGDRDGNPFVTAETLRIALMRQ
                     CELVLRHYLTEVHELGAELSLSEMLAGASADLLALAARAHDANEHRADEPYRKALIGI
                     YARLAATLKALTGTEALRHAVAPSQPYPDARAFLDDLAIIAASLASHHGAAMAEERLV
                     PLMRAAEVFGFHLSTVDLRQNSDRHEAAVAELLKTARIAPDYAALEETRKQEVLLAAL
                     GDPRCLRVPHLAYSAETESELAIFTMARVMRERLGAEAIRHAIISHTESVSDLLEVLL
                     LQKETGLMTGVLGDANARAALVAVPLFETIGDLREAPAIMRAFYALPGIAALTRAMGN
                     EQEVMLGYSDSNKDGGFFTSNWELYRASTELAKLFSGELDVQLRLFHGRGGTVGRGGG
                     PSYQAILAQPPGTVTGQIRLTEQGEVIASKYANPDIGRRNLETLVAATIEKTLLSTNE
                     TAPPAFLEAADALSAASTAAYRALVYEDSGFTEFFFAATPITEIAELNIGSRPASRKA
                     NRRIEDLRAIPWSFSWGQARLTLPGWYGFGTAVDAFLTAGGADGELLLKTMYAEWPFF
                     RTLLSNMDMVLAKADITMLKRYAALVPDPKLAARISADIRAEWQRTCDALERITGCAE
                     RLADNPALARSIRHRFPYIAPLNHLQVELIRRWRVGQHDDKTRRGILISINGIAAGLR
                     NTG"
     gene            complement(9910..10992)
                     /locus_tag="PLQ11_05580"
     CDS             complement(9910..10992)
                     /locus_tag="PLQ11_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HRK24406.1"
                     /translation="MMPSAAIPCARADKAWLAQAIRRIRADMTRSADTHLVRLDLNRS
                     LATRIYLKDESTHPTGSLKHRLARSLFLYAICNGWIGPDTPVIESSSGSTAVSEAYFA
                     RLLGLRFIAVMPRSISPAKVDLIRFYGGECHFVERGDEVYAASARLASELNGHYMDQF
                     TYAERATDWRGNNNIAESIFDQMRAEETPVPDWIVCGAGTGGTSATLGRYIRYNSYST
                     RLCLADPEASVFHRHLADPGVTTTACTSTIEGIGRGRVEPSFIPEQIDRWIAVPDAAS
                     VAAARWLSKRLGRRVGGSTGTNLVAVADLVEESEQAGRAGAIVTLLCDSGERYAASYF
                     DDAWIRAQGYDLAPETARLDRILGPA"
     gene            complement(10995..11456)
                     /locus_tag="PLQ11_05585"
     CDS             complement(10995..11456)
                     /locus_tag="PLQ11_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007694813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRK24407.1"
                     /translation="MTPEQRLAVHGIELPIPPRPVANYLPYRWAGSLLCLSGHGPRRA
                     DGSYRPGRLGRDMSIDEAYQEARLTGLNLLATAKSAIGELSRIEAVVKLLGMVNAEPD
                     FADHPKVINGCSDLFVEVLGDAGRHARSAVGMGSLPNRMAVEIEAILLIRE"
     gene            11615..12514
                     /locus_tag="PLQ11_05590"
     CDS             11615..12514
                     /locus_tag="PLQ11_05590"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRK24408.1"
                     /translation="MPAAAFHGVFPYLVSPIAAEGRVKDEVLAQLCDDLIGAGVHGLT
                     PLGSTGEFAYLNAAQKRRIVEVTVKAVAGRVPVVAGVAATTTADAVEQVRAYEALGVN
                     GILAVLDAYFPLDDAAVEAYFSAIAAATALPIVLYTNPNFQRSDLSLPVINRLSRIAN
                     IGYIKDASNNTGRLLSILTLTEGRMQVFAASAHIPAAVMLIGGVGWMAGPACIAPRLS
                     VRLYQMCRERQFDAAMPLQRGLWQLNQAFAKYNLAACIKGGLALQGYDVGDPLLPQTP
                     LGPEGRADLERVFAALSDQFGPL"
     gene            12598..>12920
                     /locus_tag="PLQ11_05595"
     CDS             12598..>12920
                     /locus_tag="PLQ11_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HRK24409.1"
                     /translation="MTAQPVQFKLASREEARQFVTANGIDTIKVGVTDVDGILRGKYL
                     SRDKFLSALEGGFGFCDVIVGWDSNDQLYDNVTYTGWHTAYPDAPVRIVPETGRLLPF
                     EGNIP"
BASE COUNT         2268 a         3944 c         4290 g         2418 t
ORIGIN      
        1 ggcgccgaag gtggagccgc ctgccgacgt tttcgccgat ccgcccaagc ctgtacgaag
       61 cgccggcgac gacgatgatg aggccgaatc gcgggcgccg gaaccggagc cggacaattt
      121 cgacgacgaa ttcgacggcg gcgtgtcgct gtcggcgatg gaagccgatc tcaagccgcg
      181 cgtgctcgaa accttcgggc gcatcgccga caattacaag aagctgcgcc ggctggccga
      241 ggccaatgtc gagaacaagc tgcaatcgaa gtcactgtcg ccggcgcagg aacgcaaata
      301 caagaagctc aaggaagaga tcgtcgccga cgtcaagtcg ctgtcgctca accagaaccg
      361 catcgatgcg ctggtcgagc aactctacga catcaacaag cggctgatcg gcctcgaagg
      421 gcggctgctg cggctggccg agagctatgg cgtcgaccgc gtcgacttcc tcaagcacta
      481 ccagggctcg gaactcgatt ccaaatggct gctgcgcatc tccaaattca ccagccgggg
      541 ctggaaggag ttcgttgcca aggacatcga ccgcatcaag gaattccgcg ccgagatcca
      601 ggcgctgtcg gccgaaaccg ggcttgaggt cagcgaatac cgcaagatcg tgctgatggt
      661 gcagaagggc gagcgcgagg cccggcaggc gaaaaaggaa atggtggaag ccaaccttcg
      721 cctcgtcatc tccatcgcca agaaatacac caaccgcggg ttgcagttcc ttgatctgat
      781 ccaggaaggc aacatcggcc tgatgaaggc ggtcgacaag ttcgagtatc gccgcggcta
      841 caagttctcg acctatgcaa cctggtggat ccggcaggcg atcacccgtt cgatcgccga
      901 tcaggcgcgc accatccgca ttcccgtgca tatgatcgaa acgatcaaca agatcgtccg
      961 cacctcgcgc cagatgctgc acgagatcgg ccgcgagccg acgccggagg aactgagcga
     1021 aaagctcgcc atgccgctcg aaaaggtgcg caaggtgctg aagatcgcca aggagccgat
     1081 cagtctcgaa acgccgatcg gcgacgagga ggattcgcac ctcggcgatt tcatccccga
     1141 tccgaacgtg atgctgccga tcgacgcggc gatccagagc aatctgcgcg agacgacgac
     1201 acgggtgctc gcctcgctca ccccgcgcga agaacgcgtg ctgcgcatgc gcttcggcat
     1261 cggcatgaac accgaccaca cgctggagga agtcggccag cagttctcgg tgacgcgcga
     1321 acgcatccgc cagatcgagg ccaaggcgct gcgcaagctc aagcatccga gccggtcgcg
     1381 caagctgagg agcttcctcg acaattgatc gcttttgcgc tgacgcattg acggtatgag
     1441 ggtctgtcct atcgttcact cgcttggagg gtgcgatgaa gcgggttctg gcggtcggtt
     1501 ttgcaatggt ctgggcggca tccggcggag cgcatgcgga gccgttgacg ccggtctaca
     1561 gttccatcca gaactgcacg ccgctgccac gtcttgagct tcccgggcga acgattgttc
     1621 gcaccgaagc gacagcagcg gcccgctgcc gcggcgtcgg cggttttgat ctggcggtgg
     1681 tcgaggagga tacgcgcagc tggctggcgg tgatcgcacc ggggggcgtg catccgctca
     1741 accgggcgat ggttcaggag ttccgccacg ggcattttcc gaccatcacc gacaacaagg
     1801 tggttgagtg gcgcgtcgac ggccaagggc aaccgcgggc gctgatcgtg cgggtgcatt
     1861 ttaccgatcc cgacatgccg gcgaccagtc ccgccgcgcg caagtcgctg ctgatggtgt
     1921 tcgacctgcg cgccctgccg ccgcgccttg caggcacggc gccggacaac gccggggcgc
     1981 ggcgggtcgc cgacggggag taatgccaac gcctgtcgag tttgaccgat gagtgttggt
     2041 gtcaggccgc gtcgccggaa atccaggcaa cggcaaagac cgaaccgctt tgagggcgca
     2101 gcagcttcac cgcttcctca aggtgcgagc cggaggtggc gacatcatcg acaagcagca
     2161 gcggcccttc aaacgaacgg atcaacgcga gcggcgggcg gcgcagattt tccttggggt
     2221 gcgattttcc cctgagcggc tgcgtgacga acgcctgaat caccggaatg tccagtttcg
     2281 cgcccaaagc tctggcaatc tccagcgaca ggcatgggcc ttcaccggaa tggccgcagg
     2341 ggatcggcac cacagcgcgg aatgcctgtg cgccgatcag ggcagacatt tcatagcgca
     2401 tttcctcggc aatcatcgac ggcatatcag acggggtgtt ggccttgagc gcccgcagcg
     2461 ccttgatctg ccagtcccgc agtgaggtcc tccaggatcg cagggtgagg taatgcacgc
     2521 cgttgcgggc gcgaatggtg cgacgatgca ccagcgtttc gtcaagaaac gcgagcgacg
     2581 gctccctggg gcgggccatg tccgactcaa cctgtcgggt gatcgacgct tccagcgcgt
     2641 tactgctggc cgtatcggca accgtgacga acaggaaata gtcgcgcgtc tcgatcgggc
     2701 tgaagggcga gatgctgagc tggaatgatt cgcgcccgct ctccgtctcg gtcaccacct
     2761 cgcagtctgg cggggaaatg cgcgattcga tcgcatggcg gtagagtgcg ctgatcgcct
     2821 cgcccatggg cacggcaagc tcgctgtggc ggcgcccgac gatgctgtcc aatggtcgat
     2881 caagcaggct tgccataggc tggttggcgg ccagtatcgt ctgatcagcc ttgagcagcg
     2941 ccgccggcat cgtacaatcg gcgaccagtt ggctggccgt atccagcgtt atcgccgcgg
     3001 tcaccccggc tgacggatcg gtcagcagtg gcgcatggcg cgagaattcc tcgcggatca
     3061 gcgccttgat gttgtccagc agctttcgct tggcaggcgt tggccgtttg cctggttcag
     3121 gatcgccgat cagcagagcc agatgatagg cagcggccac gacaaccggc gtccgcagga
     3181 aaaagccaag gcggggcagc ccaagcaggg tcgcgatcgg ctgatagtgc ggctcaccgg
     3241 aagcatcggg ctgcaccacc tcggcgtcgc gcgcgtaagg tgcgatactg aaatccagct
     3301 gccggctgcg gtagggctgc cgggtgcccg aagaagcgat gacacgactg acccctgcgg
     3361 agtgcagcac cagcaaggcg gactgcgctc caaaaagcct gcgggccaga tcgcaggcgc
     3421 gaccgggagc ctgcacctcg ccgaacgccg gttgcctttc gtgactctct tcaagcttca
     3481 tgcgcgcacc gaccgcctct ttccggcaga agccgccccg atgatagcct tggcggagat
     3541 ggcatgacac tgtagcatct tggaaaaacg atcaagcgaa cactctggca ggcgcatccg
     3601 atgtcatttt ttgcaaaact gttcggcaag tccgcagctc aggcacccga gccggtcaac
     3661 gaggattacc ggagctatat cctgacggca acgccttatc aggaggccgg gcagtggcag
     3721 atgtgcggag tgatctcgaa agagatcggc aatgagacga agcagcatcg cttcgtgcgc
     3781 gccgaccggt ttcccagccg tgaggatgcg gtggaattca cgctgaagaa agcgcggcag
     3841 atcgtcgatc tcaacggcga ccgaatcttc tcctgaaccc cctgtgtgta accggcgggg
     3901 cgccggttgc catgcttgaa aagccgttcc tgcgtcgctg cactgaggcg cgctgtcacg
     3961 tctgattcgc gtcgggcggg gcaacaggcg cgcatgtgac cggagcgata ctgatgaccg
     4021 atgcaatcct gagggcacca ccatcaagcg gccagaatcc ggtctatagc ctgctgttca
     4081 gccgctcctt catgggacgg ccctatctga tctcgccggt cgccgatgtg ctgatggcgg
     4141 gcggtggcgc attgttcctg ctcgccgtgc tgtctctgac cttgccaacc caggccggcc
     4201 gggccgcgcc ggaagcgatt gcgacgacag cggcgatttt cgccacggtc agctatttca
     4261 tcaactatcc gcatttcatg gccagctatt cactgctcta tggatcgctg ggccagcgca
     4321 tggcggccta ttcgccgggc cagatgacct ggtggcggac ggtcaatgcg gcgtttgtcg
     4381 cgccggccgt gctggtggcg ttctttgcct attgcgtggc ctggcggcgt gtcgatctca
     4441 tgggctgggc ggttcaggcg atgtttttct tcgtcggctg gcattatgtg aagcaatcct
     4501 acggcgtgtt tgtcgtgctc tcgggcatga aaggcatcta ctacaaccgc tggcaggtgc
     4561 tggctctgaa ggggcatgtc tattcgctgt ggctctacag ctggttctcc tcgggtgcgg
     4621 tgattctcga aggcatgtcg ccgcggccga tgaatttcca ctcgatttcc tatgacgggc
     4681 tgagcctgtt caagacgcag gggctggttg atttcctgtt ctggccgacc gtgttttttg
     4741 cccttgccgg atgggcggcg gtgctgtgga actggtatgc gaccggcaag cggccatcgc
     4801 tgtccggcct tctcggctat acctcgatgt atacgctgct ggccttcgtc cggctgcatc
     4861 cgctgtttgc ctatgcggcg ccggcgttcc attcgctgca atatctgctg ttcgtctatg
     4921 cctacaagcg cggagaattc caccttgaag gtcaggacca tgccgtgccc gataccgggg
     4981 ccaagccgcc gtggctgcgg ctgctgacct atttcctcct gatgtgcgcg accggcgcgc
     5041 tgttctttga tgtcctgccg ttctacttcg acaacaagag cacacgctgg ggcctgccgc
     5101 tggtggcgac gccgatcttc caccttttca tcaacgtgca ccactatttc atcgattcgg
     5161 cgatctggag gcgcgacaac cgcgatgtca gccgcttcct gttctggcgg gcacaggcgg
     5221 agcggcgatg attgatctta gcgatcaaac ctttcaattt gacaggcgct gatgccggcc
     5281 taacctgacc ggatgcaggg ttcttcttcc gcgccggagc atttgccgga ttccgcctat
     5341 gcctggcgca ggctcgcggc ctgcgtgctg gtcggaacca tggcgtcggc gggcatgtgg
     5401 accgtggtgg tggcgctgcc gacggttcag gccgaattcg gcacggcgcg ggccggtgcg
     5461 tccttgccct acaccgcgct gatgatcttt ttcggtgtcg gcaccatcat catggggcgg
     5521 gcggccgacc ggtttggcct gaccattccg gtcattgtcg ccgccgtggc gatcgggctc
     5581 ggttatgtca tttccggaat ggccacctca ctgaccatgt ttacgctggc gcatggcgtg
     5641 ctgatcgggt ttggcggcgg ggcgggattt gcgccgctga tgtccgatat cagccattgg
     5701 ttcgtcaggc ggcgggcgtt ggcggtcacg ctggctgcct gcgggtcgta tctcgccggc
     5761 acgctgtggc cgctggtgat gaacctgacc atgccgctct acggctggcg ggccacccat
     5821 gtcggcatcg gcctggcggt catggtgatc atgctgccgc tggcattcat gttccgggtg
     5881 aagccttcgc aggccagtct tgccgcttcg gagagtgcca cggctgcggc acgcgccgat
     5941 ctcggcattc ctcccggccg gttgcaggcg atgctgacgg cggcggggct gtcctgctgc
     6001 atcgccatgt cgatgccgca ggtacatatc gtcgcctatt gcggcgatct cggctacggg
     6061 gtggcgcgcg gggcggaaat gctgtcgctg atgctgggac ttggcatcgt cagccgcatt
     6121 gcctcgggct ttctggcaga ccggatcggc gggccggcga cgttgatgat cggctcgctg
     6181 atgcagggga tggcgctgct gctctatctg ttttttgacg ggctgagctc gctctatctc
     6241 atttcggcga tcttcggcct gttccagggt ggcattgtgc cgatgtatgc ggtgatcgca
     6301 cgcgaattcc tgccaccgcg cgaggccggc gcaagaatcg gcatcatcat ctcggccacc
     6361 attgtcggaa tggcggtggg cggctatctg tcggggctga ttttcgacct tacaagctcc
     6421 tatcgcatgg ccttcctgca cggacttttg tggaacttcg tcaatctggc actgaccgga
     6481 tggctggttg cgcgacgccg gcaggggatg gcgctggctg gggcggcacg ctgaacccat
     6541 cggtcacgag aagtgaccga tgacaaattt gaattttcat tatttttcaa tgagatatga
     6601 aaattcaaaa caggaaccaa gggtcaattg tcatgaccct tggcatgaca ggatggacgc
     6661 aacgatgacc ccgacacccg agcccaccga tccgcgcggg gatctgaccg tacgcaccat
     6721 cgccatgccg gcggatacca acgccaatgg cgatattttc ggcggctggg tgatgtcgcg
     6781 gatggatcag gcgggcggta tcgccggcgt gcgccgcgcc cgtggccgcg tcgtgaccgt
     6841 caaggtgcag gcgatgacct tccaccgccc ggtgcgggtc ggtgatgtgc tgacggtcta
     6901 tacccgcatc gggcggatcg gccgcacctc gatggatatc gagatcgagg cctgggctcg
     6961 ccggtttctc accggcgagg aggaaaaggt gaccgacggg gtgttcacct ttgtcgccat
     7021 taccgacgag ggaaagccgc ggccgattcc gcggtgaagc tcccttgcgc cggtctcacc
     7081 cggtattcct gaggcccgcc gcgatgccgt tgatcgaaat gagaatgccg cgccgggtct
     7141 tgtcgtcgtg ctggccgaca cgccagcggc ggatgagttc cacctgcagg tggttgagcg
     7201 gcgcgatata ggggaagcgg tggcggatgg agcgtgcgag cgccgggttg tcggcgagcc
     7261 gttcggcgca gccggtgatg cgttcgagcg catcgcaggt gcgctgccat tcggcgcgga
     7321 tgtcggcgga aatgcgcgcc gccagcttcg ggtccggtac cagcgcagca tagcgcttga
     7381 gcatggtgat gtcggccttg gccagcacca tgtccatgtt cgacagaagc gtgcggaaga
     7441 acggccattc ggcatacatc gtcttgagca gtaactcgcc atcggcgccg ccggcggtga
     7501 ggaacgcatc gaccgccgtg ccgaacccat accagcccgg cagggtgagg cgtgcctggc
     7561 cccaggagaa tgaccaggga atggcgcgca gatcctcgat gcggcggttg gccttgcgcg
     7621 aggccggacg cgagccgatg ttgagttcgg caatctctgt gatcggggtg gcggcaaaga
     7681 agaattcggt aaagccggaa tcctcgtaga ccagcgcacg ataggcggcg gtggaggcgg
     7741 cggaaagggc atcggccgct tcgagaaagg ccggtggtgc cgtctcgttg gtcgaaagca
     7801 gcgtcttttc gatggtcgcc gccaccagcg tttcgaggtt gcggcggccg atgtcggggt
     7861 tggcatattt ggaggcgatc acctcgccct gttcggtgag gcggatctgt cccgtcaccg
     7921 tgccgggcgg ctgggcgaga atggcctgat agctcggtcc gccgccgcgc cccaccgtgc
     7981 cgccacggcc atggaacagg cgaagctgca catcgagttc acccgagaac agtttcgcca
     8041 gttcagtcga ggcccggtat agctcccagt tcgaggtgaa gaagccgcca tccttgttgc
     8101 tgtcggaata gccgagcatc acctcctgct cgttgcccat cgccctggtc aaagcggcga
     8161 tgccaggcag cgcatagaag gcgcgcatga tggcgggagc ctcgcgcagg tcgccgatgg
     8221 tttcgaacaa cggcacggca accagcgcgg cgcgcgcatt ggcatcgccg aggacgccgg
     8281 tcatcagccc ggtttccttt tgcagcagca gcacttccaa cagatcgctg acactctcgg
     8341 tatggctgat gatggcatgg cggatcgcct cggcgccgag ccgctcgcgc atcacccgcg
     8401 ccatggtgaa aatcgccagt tcgctctcgg tctcagcgga ataggcaaga tgcggcaccc
     8461 tgaggcagcg cggatcacca agcgcggcga gcaatacctc ctgttttctt gtttcctcca
     8521 gcgccgcata gtccggcgcg atgcgggcgg ttttcagcaa ttccgccacc gccgcctcgt
     8581 gccggtcgga attctgcctg agatcgacgg tcgacagatg gaagccgaac acttcggccg
     8641 cccgcatcag cggcaccagg cgttcctcgg ccatcgccgc gccgtgatgg ctcgccagcg
     8701 aggcggcgat gatggcaaga tcatcaagaa acgcgcgtgc atctggataa ggttgcgacg
     8761 gggccaccgc atggcgcagc gcctcggtgc cggtcagcgc tttcagcgtg gccgccaggc
     8821 gcgcgtagat gccgatcagc gccttgcgat agggttcgtc cgcgcggtgc tcgttggcgt
     8881 catgggcgcg ggcagcgagg gcgagcagat ccgccgaggc gccggcgagc atctcggaca
     8941 gcgacagttc cgcccccagt tcatgcactt cggtcagata atggcgcagc accagctcgc
     9001 actggcgcat cagcgcgatg cggagtgttt cggcggtgac gaacgggttg ccgtcgcgat
     9061 ccccgccgat ccagcttccc atccgcagga acggggcgac gcgcgcgccg gtcaactcct
     9121 ccagccggcc gtagagacgc ggaatctcct tgaggaacgt ggtccgataa tagctcaggg
     9181 cgttgtcgat ctcgtcctcg acgctgagct tggaggtgcg caacagtcgc gtctgccaca
     9241 attgcgcgac gcgggctttc aggctggcaa ggttgcggtc acgatcgcgc gctgtcctca
     9301 ggttctcgcg gtcgaccagc agatgggcga tggcgcgctc ggcatcgaga atggacttgc
     9361 gctgcacctc ggtcggatgg gcggtgagca ccggcgacag ccaggccgtc tccagcatgg
     9421 cccgaaccgt atccgccgcc attccggcat cgccgatccg gtcaaagcag cgctccaggc
     9481 tgccgtcctg cgggcggtct cccgccgctt catgcgcctc gcggcggcgg atgtggtggc
     9541 ggtcctcggc gatgttggcc agatgcgaga agtaggtgaa ggcgcgcacg acgctgaccg
     9601 ccttgtcgga agggatggcg cgcagcagca tgtcgagcgt ttcggcggcg ctctgggaat
     9661 tgtcgcggct gatcgccacc gacaggcgac ggattgtctc gatcaggtcg aaaacgtccg
     9721 cgccttcctg ctcgcgcaca gtatcgccga gaatctgccc cagcagccgg atgtcatcaa
     9781 tcagcgactg atccttgtcg gcggagcggg gttgatccct ctgggtcatg gcaggtcctc
     9841 gggcagcatc gggccaatgg tcctgtcctg ccatcactgg catggccaag gcaaggtcgg
     9901 agcagggcgt caggccgggc cgagaatgcg gtcaagccgg gcggtctctg gagcgagatc
     9961 atagccttgc gcccgaatcc aggcgtcgtc gaaatagctc gccgcatagc gctcgccgga
    10021 atcgcataac agcgtgacga tggcgccagc acggccagcc tgctcgctct cctcgaccag
    10081 atcggcaacc gcgacaagat tggttccggt cgacccgcca acgcggcgcc cgagacgctt
    10141 cgacagccag cgcgcggcgg cgacgctggc cgcatccggc acggcgatcc agcggtcgat
    10201 ctgttcggga atgaacgagg gctcgacccg gccccggccg attccctcga tggtcgaggt
    10261 gcaggcggtg gtggtcacgc ctggatcggc aagatgacgg tggaacaccg atgcttcggg
    10321 atcggcaagg cagaggcggg tggaatagga attgtagcgg atatagcgac cgagggttgc
    10381 cgacgtgccc cccgtgccgg caccgcagac gatccagtcc ggcaccgggg tttcctcggc
    10441 gcgcatctga tcaaagatcg attcggcgat gttgttgttg ccgcgccagt cggtggcccg
    10501 ctcggcatag gtgaactggt ccatgtagtg accgttcagt tcgctcgcca gccgggctga
    10561 ggcagcatag acctcatcgc cacgctcaac gaaatggcat tcgccaccat agaagcggat
    10621 gaggtccacc ttggccggcg agatcgaacg cggcatcacg gcaatgaacc tgaggccgag
    10681 caggcgggcg aaataggctt ccgacacggc ggtcgagccg gaggaggatt cgatcaccgg
    10741 cgtgtccgga ccgatccagc cgttgcagat cgcatagaga aacagcgagc gggcgagccg
    10801 atgtttgagg ctgcctgtcg gatgggtcga ttcatccttg agatagatgc gtgtggccaa
    10861 ggaccggttg agatcaagcc gcaccagatg ggtgtctgcc gagcgtgtca tgtcggcgcg
    10921 gatgcggcgg atggcctggg ccagccatgc cttgtcggca cgggcacagg gtatggcagc
    10981 gctgggcatc atggtcactc gcggatcagc aggatggctt caatctcgac cgccatgcga
    11041 ttggggagcg accccatccc gacggccgag cgggcatggc ggccggcatc gcccagcacc
    11101 tcgacgaaca ggtcggaaca gccgttgatc accttcggat ggtcggcaaa gtcaggctct
    11161 gcattgacca taccgaggag cttgaccacc gcttcgatgc gcgacaattc gccgatggcg
    11221 gattttgccg tcgccagcag gttgaggccg gtcagccgcg cttcctgata ggcttcgtcg
    11281 atgctcatgt cgcggcccag ccggccgggg cgatagctgc catcggcgcg gcgcggcccg
    11341 tgcccggaca gacacagcag cgagccggcc cagcgatagg gcaggtaatt ggcgacgggg
    11401 cggggcggta tcggcagttc gatcccgtgc acggccagcc gctgctccgg tgtcatcatt
    11461 ccgctgctcc catgcgttcg gacatgccac accattcacg acgggggacc cagaacgttg
    11521 tcggcggttc gccggccaag gtcaatcact cttgctcccg agcgggtttg ctacgacaag
    11581 aattgtctgc ccggaccttg ccctggagtg tgtcatgccc gctgccgcct ttcatggagt
    11641 atttccctat ctggtctcgc cgattgccgc cgagggcagg gtgaaggacg aggttctggc
    11701 gcagctctgc gatgatctga tcggggccgg cgtgcatggg ctgacgccgc tcggctcgac
    11761 gggggaattt gcctacctca atgccgcgca gaagcggcgc atcgtcgagg tgacggtgaa
    11821 ggcggtggcg gggcgggtgc cggtggtggc gggcgtggcg gcgaccacca cggcagatgc
    11881 ggtcgagcag gtgcgggcct acgaggcgct gggggtgaac ggcatcctgg cggtgctgga
    11941 tgcgtatttc ccgctcgatg atgcggccgt cgaagcctat ttctccgcga tcgctgccgc
    12001 tactgctctg ccgatcgtgc tctacaccaa cccgaatttc cagcgctcgg acctgtcgct
    12061 gccagtgatc aaccggctct cgcgcatcgc caatatcggc tacatcaagg atgcgtcgaa
    12121 caataccggg cggctcttgt cgatcctcac cctgaccgag gggcgcatgc aggtgttcgc
    12181 ggcctcggcg catatcccgg cggccgtgat gctgatcggc ggggtcggct ggatggcggg
    12241 tccggcctgc atcgcgccgc gcctcagcgt caggctctac cagatgtgcc gggaacgcca
    12301 gttcgacgcc gccatgccgt tgcaacgcgg attgtggcag ctcaaccagg ctttcgcgaa
    12361 atacaacctg gcggcctgca tcaagggcgg cctcgccttg cagggctacg atgtcggcga
    12421 cccgttgctg ccgcagacgc cgctcggacc ggaggggcga gccgaccttg aacgggtctt
    12481 tgccgcgctt tccgatcaat tcgggccgtt gtgatcaact gtatgccggc tatggtcaag
    12541 cacggtcggc gcagatagca ttcgacatat gaaaagaacc atgcacggga ggcgttcgtg
    12601 acggcccagc cagtccagtt caagcttgca tcgcgcgagg aggcgcggca gttcgtcacc
    12661 gccaatggca tcgacacgat caaggtcggg gtgaccgatg tcgatggcat cctgcgtggc
    12721 aaatatctca gccgcgacaa gttcctgtcg gcgctcgaag gcggtttcgg cttctgcgat
    12781 gtgatcgtcg gctgggattc caacgaccag ctttatgaca acgtgaccta caccggctgg
    12841 cacaccgcct atcccgatgc gccggtgcgg atcgtcccgg agaccgggcg gctgctgccg
    12901 tttgagggca acatcccgtt
//
LOCUS       DAOMXG010000057        12745 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_2690_length_12745_cov_8.780615, whole genome shotgun sequence.
ACCESSION   DAOMXG010000057 DAOMXG010000000
VERSION     DAOMXG010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 12745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12745
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2690_length_12745_cov_8.780615"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="PLQ11_05600"
     CDS             complement(<1..555)
                     /locus_tag="PLQ11_05600"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRK24410.1"
                     /translation="MRQRHRDHGSSKVLKLLGAAMIAGMSGACSSDILRFSDTPFANP
                     FNGKADQATTGSVNAPLAKVRASSLGMPQTPEPYQPFPSAVGGTTASQAAMTQVAAAG
                     NQPVLGSVAGWSATGGTPVTAGAGDTVEALSGRYGVPVQALRQINGIKGQPSAGQQVI
                     IPAYNPGMARSAAAAPSVTRTAPPA"
     gene            complement(711..1211)
                     /gene="gpt"
                     /locus_tag="PLQ11_05605"
     CDS             complement(711..1211)
                     /gene="gpt"
                     /locus_tag="PLQ11_05605"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HRK24411.1"
                     /translation="MQPLPDKAFPVSWDQFHRDARALAWRLAGAGPFRAILCITRGGL
                     VPAAIIARELELRLIDTICIASYHSYSAQGDLQLLKGASPELIGEDGGKGVLVLDDLV
                     DTGKTVKLTRELLPNAHYATVYAKPLGRPLVDTFVTEVSQDTWIYFPWDMGFAFQPPI
                     RKGAAG"
     gene            complement(1231..1998)
                     /locus_tag="PLQ11_05610"
     CDS             complement(1231..1998)
                     /locus_tag="PLQ11_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007566757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HRK24412.1"
                     /translation="MSTTVPITAALLVIGDEILSGRTKDRNIGYIADYLTNVGIDLKE
                     VRVVPDDEAEIIDAIRALKGRYTYLFTTGGIGPTHDDITADCVAKAFGVGIDHDPRAV
                     AILRQRYKPEDLNEARLRMARIPDGADLVDNPISKAPGFMIGNVIVMAGVPQIMQVML
                     DNVAPKLKTGVKMLSRSVETGGLPEGVYAEVLGAIAKAHPGAIIGSYPSFDGKTFLNR
                     IVMRGRDESLLIAVEKQVIDALNTLHEAQGSSYRLPL"
     gene            complement(2058..2885)
                     /locus_tag="PLQ11_05615"
     CDS             complement(2058..2885)
                     /locus_tag="PLQ11_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010685945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HRK24413.1"
                     /translation="MPDLDRSFGSFAPTGFMARLLETTRALPTHGLGKTLGFALRRLA
                     SMMLHGKPLDVEAYGAKFRLYPYNNVCESRILFFPRQFDEEERRLLAERMKPGFHFVD
                     IGANIGGYALAVAALAGPAARILAVEPLPEIFSRLAFNVRQNPFGTVKALELAVADKD
                     GDMTLFIDPYNQGESSLKIITTAEGTAVRVPARKLTTILREERFERIDALKLDTEGAE
                     DLILECFFGEADERLFPRLIILQSTPERWNINLFALIEQHGYRIIAETRNNVVYERG"
     gene            3054..3878
                     /gene="map"
                     /locus_tag="PLQ11_05620"
     CDS             3054..3878
                     /gene="map"
                     /locus_tag="PLQ11_05620"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009494106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRK24414.1"
                     /translation="MHTDVKTKRRPGHIRQHGPEAFAAMRKAGRLTAEALDLMTEWVI
                     PGVTTAELDRRAFAFAMDHGAYPAPLNYRGYTHSICTSINHVVCHGIPDDKPLREGDI
                     VNVDVTLIVDGWHGDSSRMYYVGEVPRKAARLCEVTYESLMRGIAAVRPGATTGDIGH
                     AIQTFAEAERCSVVRDFCGHGLGELFHDEPNILHFGRPGEGPVLKPGMLFTIEPMINL
                     GKPQVKILSDGWTAVTRDRSLSAQFEHTIGVTETGCEIFTVSPGGLHHPPYAKAGA"
     gene            3875..4567
                     /gene="radC"
                     /locus_tag="PLQ11_05625"
     CDS             3875..4567
                     /gene="radC"
                     /locus_tag="PLQ11_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HRK24415.1"
                     /translation="MTRGDAAENLNAGHRDRLRRSFRAAGAEALPDYELLELLLFRSI
                     PRRDVKPIAKLLIRRFGSFAEVLGAPLERLTEVEGVGAATALDLKIIAAASQRMARGD
                     ISKRTTLSSWSALIDYCRVAMAFSEREELRILFLDKKNGLIADEVQQTGTVDHTPVYP
                     REVLRRALELSATAIILVHNHPSGDPTPSTSDIRMTQDVVAIAKPLGIAVHDHIIIGR
                     NGHASFRGLGLL"
     gene            4692..6839
                     /locus_tag="PLQ11_05630"
     CDS             4692..6839
                     /locus_tag="PLQ11_05630"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRK24416.1"
                     /translation="MDALKNPGSRTLSTSLSVVPILLVSFAVIGSMSFLAKTAFERNE
                     RQAILTEQRRIDMALEHEASEFADQIQREALLLAQPESGKPIDMERLSTYLASEHRLD
                     FVAVYSRAGKLISIRSSRPEILERRLKASDELIAERETLKTPFALRRIGMSSTLVEPR
                     AIISMAAVNVGDQFQLVVARRLEDRTLTAIGKLLLLADLRFAETDLTSPASPETGMTL
                     VWNRINDGSFNLQSLASIGFMGIAVIGIYGLLVYLHIRRVTRELEQSEAQAQHQAGHD
                     PLSGLPNRSLFHRALNTELARVNRDGGGLAVMYLDLDKFKEVNDQFGHEIGDKLIIQV
                     AGRLGQLVRGADTVARFGGDEFAIIQVGVRTIDDVETLARRVLDELRRPFRIDDFEVN
                     IGCSIGVSVAPENGNDSVQLMRYADIALYRAKNEGRNRYSFFEHQMNETMRLKKIIED
                     DLRAAIDNERLVLHYQPIVTADGSQIIGLEALVRWNHPESGLIPPNEFISIAEERGLI
                     SALGEWVLRRACLDTKHWPGVSVSVNVSPVQFRQRDFVATVARILAETGFDPHRLELE
                     LTEGVVVEDADQAEAAIMDLRAMGVRIALDDFGVGYSSLIYLRRFAFDRIKIDKSFLE
                     SMEGTGESAILIHSIVHLGRALGLEVTAEGVETEEQQRFLQAVGCHHLQGYYFARPCP
                     AAEIAGVLEKAQAMPNEGRLADKPNPASPRAAA"
     gene            complement(6975..7595)
                     /locus_tag="PLQ11_05635"
     CDS             complement(6975..7595)
                     /locus_tag="PLQ11_05635"
                     /inference="COORDINATES: protein motif:HMM:NF017833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HRK24417.1"
                     /translation="MSDPVAAQSLSTILTGIARTAGSSSEAKVSLNELVAGFGNRAFG
                     LLFLMFGLPNCLPMPPGLPVLCGVVVMVVALQMLLGMSQLLLPGWLGNRQIDRAMLNR
                     VVEKATGPLRWMERLAKPRFPLLSGDLMRRAVGLAGLALGVALMAPIPVFGGIPAGIA
                     VTILGLAFTQRDGLLMVFGVLVATPVALAVTSAMIYALFQGAAAFF"
     gene            7880..8545
                     /gene="bcsS"
                     /locus_tag="PLQ11_05640"
     CDS             7880..8545
                     /gene="bcsS"
                     /locus_tag="PLQ11_05640"
                     /inference="COORDINATES: protein motif:HMM:NF028346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsS"
                     /protein_id="HRK24418.1"
                     /translation="MAMPALALDWTDALSWDAVFATADFDNRTAFVAQGGKKQLSMPD
                     EGAKMTLAFVSGVSLSDIARVRRGKLSVVEIDRYSRLLLGLERSAGPVYTAVAIGPSL
                     ALEKPAVRPGRQRFGVALDAQVWLRPDTATYWAFALTGDTAARTLWARGRLGYRPEGW
                     VMAAGPELGAAADAQSGKTRVGLHLGEITLGPFRFDVATGRQWDTRRRAGSYATLAVV
                     GRY"
     gene            complement(8594..10216)
                     /locus_tag="PLQ11_05645"
     CDS             complement(8594..10216)
                     /locus_tag="PLQ11_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding/glycosyltransferase family
                     2 protein"
                     /protein_id="HRK24419.1"
                     /translation="MKFGSVPVAAAVGGILAHAVRQGGVVLRKGTRLRDNDVLLLEKA
                     GVREVVIARLEEGDVDEDSAALAIAETLAGGGVAVERPFTGRANLTAEAAGVFRVNAE
                     VIHALNGIDGAITVATLPDFRAVRAGEMVATVKIIPFAVDHALVNGAILAAGSGNGAL
                     SVTAFRPGRVALISTTLPGLKASVIDKTAAVTAARLAAAGTTISADQRVPHEMNALAD
                     ALARQAAANDVVIVFGASAITDLADVIPAAIRQAGGRVDHFGMPVDPGNLLLVGRIGD
                     ARVIGAPGCARSPKENGFDWVLQRMLAGFDVTRFDIQAMGVGGLLTEIASRPHLREPR
                     SPAAPTVIPAQASLGVIVLAAGRSTRMEGANKLLLPVDGVAMVRRSVEVALAAGVGPV
                     SVVTGHEADRVMAALAGLPVRFVANADFVQGMATSLRAGLAAQPEDVAGVLVMLADMP
                     LVAPAVLQRLAGVFGEHPGVEAVVPTLVGERGNPVLIGRALFGRIAGLTGDQGARKLI
                     EAAGADVIEVPVEDAGIARDFDTPEAFGRLKR"
     gene            complement(10213..10911)
                     /locus_tag="PLQ11_05650"
     CDS             complement(10213..10911)
                     /locus_tag="PLQ11_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HRK24420.1"
                     /translation="MKLEVLAALNAERAARRPALLVTDPESGEVRLVRAADLVSLPLA
                     GTELGEATAKALRQGRSAMAMVAGRRLFLTVQVPPVRIVVIGAVHIAQALAPMVRQVG
                     FDLTIIDPRTAFATPERFPDVALLTEWPEAALTRLGLDRYTALVALTHDPKIDDPGLV
                     AALAAGCFYIGALGSRKTHAGRLARLAAAGCDEAASARIHAPIGLDIGAISPAEIAVS
                     ILAEIIGALRTMPP"
     gene            complement(10908..11231)
                     /locus_tag="PLQ11_05655"
     CDS             complement(10908..11231)
                     /locus_tag="PLQ11_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HRK24421.1"
                     /translation="MLASDDALLTKAEAWARAGRGVALATVVETWGSAPRPVGSHLVI
                     DHEGNFLGSVSGGCVEGAVVTEALEVIASGKPAMLSFGVADETAWRVGLSCGGRIAVY
                     VEPVA"
     gene            complement(11319..12542)
                     /locus_tag="PLQ11_05660"
     CDS             complement(11319..12542)
                     /locus_tag="PLQ11_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018095059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRK24422.1"
                     /translation="MQAGAVEAGTGRLAENIAYFGRALRSAGLPVGPGAVLDAVAAVE
                     AAGIGHRADFHATLQAVFVKKREHMAVFDQAFQIFWRKRSLMERMMAAMMPVAPGEPD
                     KPRQEALRRVSEALYDDRNRAPQEVEQQKMLDARLTVSDLEVFKRRDFEQMSAAEIEE
                     AKRAVARLVLPLDRVVTRRMRADPNGLRVDPRASLRATLRSGAGGIALKFRAPLTVHP
                     PIVALADISGSMSRYAQVFLHFLHALGRADRKVHGFVFATELTNISRALAAHRDPDLA
                     LAATARMVRDWDGGTRIGRALSDFNRLWSRRVLGQGAIVLLLTDGLERQETAELSFAL
                     DRLRRSCRRLIWLNPLLRYDGFEARAAGIRAMLPHVDEFRSLHNLASMADLCRALTGE
                     PAEAAADPRRFRLPE"
     gene            complement(12542..>12745)
                     /locus_tag="PLQ11_05665"
     CDS             complement(12542..>12745)
                     /locus_tag="PLQ11_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRK24423.1"
                     /translation="REDLFKAPGVAETLDWASALVELDAVALDPALVSDTLGVLLKYQ
                     DDIAKIQGTKAKDLIDRARAEAR"
BASE COUNT         2318 a         4310 c         3928 g         2189 t
ORIGIN      
        1 agccggcggc gccgtgcgcg taacggacgg cgcggcagcg gccgagcgcg ccatgcccgg
       61 attgtaggct ggaatgatga cctgctggcc ggcgctgggc tggcccttga tgccgttgat
      121 ctggcgcagc gcctgcaccg gcacgccata gcgacccgac agcgcctcga ccgtatcacc
      181 ggccccggct gttaccggcg ttccgcctgt tgcgctccaa cctgcgacgg agccaagcac
      241 cggttgattg ccggcggcgg caacctgcgt catcgcggcc tgcgaggccg ttgtgccgcc
      301 gaccgccgag gggaacggct gatagggctc cggcgtctgc ggcatgccaa gcgaggaagc
      361 cctcaccttg gcgagcggcg cattgaccga gcccgttgtg gcctgatcgg ccttgccatt
      421 gaacggattg gcgaacgggg tatcggagaa tctcaggata tccgaactgc acgcgccgga
      481 catgccggcg atcatcgcag ctccgagaag cttgagaacc ttgctggatc cgtggtcgcg
      541 gtgacgctga cgcatactcg cactcttttc gatttgagcc gaaaaaagta agaccacgtt
      601 ggggttacga aacggttgac ggggccgccc cggcattcac caaaccggca ttttctgccg
      661 ctggtgttat ccgccgttca tcatggttga tgcttggtga acgccccgcg ctatcccgcc
      721 gcccccttgc ggatcggcgg ctggaaggca aagcccatat cccagggaaa atagatccag
      781 gtatcctgcg acacttcggt cacgaatgta tcgaccagcg gccgccccag cggcttggca
      841 tagaccgtgg cataatgggc attgggcagc agttcgcgtg tcagtttcac cgtcttgccg
      901 gtatcgacca gatcatcgag caccagcacg cccttgccgc cgtcctcacc gatcagctcg
      961 ggactggcgc ccttgagcag ttgcaggtct ccctgcgcgc tgtagctgtg ataggaggcg
     1021 atgcagatcg tgtcgatcag ccgcagttcc agctcgcgcg cgatgatggc cgccggcacc
     1081 agcccgccgc gggtgatgca caggatcgcc cggaacggcc ccgccccggc cagacgccag
     1141 gccagcgccc gcgcatcgcg atgaaactgg tcccacgaca ccgggaacgc cttgtccggc
     1201 aacggctgca tgatcgaact ccgcaatgcg tcagagtggc aatctgtagc ttgagccctg
     1261 cgcttcatga agggtattca gcgcgtcgat cacctgcttt tccaccgcaa tcagcagaga
     1321 ctcgtcgcgc ccccgcatga cgatgcggtt gagaaaggtt ttcccgtcga aggacggata
     1381 ggatccgatg atggcgccgg gatgggcctt ggcgatcgct cccagcacct cggcatagac
     1441 accttcaggc aggccaccgg tctccaccga gcgggacagc atcttcacgc cggtcttgag
     1501 cttcggcgcg acattgtcga gcatcacctg catgatctgc ggcacgccgg ccatcacgat
     1561 cacattgccg atcatgaatc ccggcgcctt ggaaatgggg ttatcgacca gatcggcgcc
     1621 atcgggaatg cgcgccatcc tcagcctcgc ctcattgagg tcctcgggct tgtagcgctg
     1681 tctgaggatc gccaccgcgc gcggatcatg gtcgataccg acgccgaacg ccttggcgac
     1741 gcaatcggcg gtgatgtcgt catgggtcgg gccgatgccg ccggtggtga acagataggt
     1801 atagcggcct ttcagcgcgc ggatcgcgtc aatgatctcg gcctcgtcgt ccgggacgac
     1861 ccgcacctct ttcaggtcga taccgacatt ggtcagatag tcggcgatat agccgatgtt
     1921 cctgtccttg gtgcggccag agaggatctc gtcgccaatg accagcagcg cggcggtgat
     1981 ggggactgtc gtgctcatgc ccccttatgc agtcgccccg cctggtgcgc aagggcgctg
     2041 acccgcaacc gaacgcttca gccgcgctcg tagacgacgt tgttgcgggt ttcggcgatg
     2101 atgcgatagc cgtgctgttc gatcagcgcg aacagattga tgttccagcg ctccggggtc
     2161 gattgcagga tgatgagccg ggggaacagc cgctcgtcgg cttcgccaaa gaaacattcg
     2221 aggatcaggt cctccgctcc ctcggtatca agcttcagcg catcgatccg ctcgaaccgt
     2281 tcctcgcgca ggatcgtcgt cagcttgcgc gccggcacgc gaacggcggt cccttctgcg
     2341 gtggtgatga tcttgaggct cgattcgcct tgattgtagg ggtcgatgaa caaagtcatg
     2401 tcgccatcct tgtcggcgac ggcaagttcg agcgccttga cggtcccgaa cgggttctgg
     2461 cgcacgttga aggcgaggcg ggagaaaatc tcaggcagcg gctctacggc caggatgcgg
     2521 gcggcaggcc cggcgagcgc ggccacggcc aaagcgtagc caccgatgtt tgcaccgata
     2581 tcgacgaaat ggaaccccgg cttcatccgt tcggcgagaa gccggcgctc ctcctcgtca
     2641 aactggcgcg ggaagaacag gatccggctt tcgcagacgt tgttgtaggg atagaggcgg
     2701 aatttcgcac cataggcctc gacatccagc ggtttgccat gaagcatcat gctcgccagc
     2761 cgtcgcagcg cgaagccgag ggtcttgcca agcccgtggg tcggcagcgc ccgcgtcgtt
     2821 tcaagcagtc gcgccatgaa cccggtgggc gcgaaactgc caaagctccg atcaaggtca
     2881 ggcatgaaat cccggatggt aagaaagccg ctgggactgc ggcggcgttg cccttcctct
     2941 taccggtcga agctgcactg ataaagcccc ggatgcgtgt ccgcccggca agccaggggt
     3001 tggccttgcc tccgtgtcgg cgtatggggc attctgatca cggacacgtt gctatgcaca
     3061 cagacgtcaa gaccaaacgc cgccccggcc atattcgcca gcatggcccc gaagccttcg
     3121 ctgccatgcg caaggccggg cggctgaccg ccgaggcgct ggatctgatg accgaatggg
     3181 tcatccccgg cgtgaccacg gcagagcttg accggcgggc ttttgccttt gccatggacc
     3241 acggcgccta ccctgccccg ctcaactatc gcggctacac ccattcgatc tgcacatcga
     3301 tcaaccatgt cgtctgtcat ggcattcccg acgacaagcc gctgcgcgag ggcgatatcg
     3361 tcaacgtcga cgtgacgctg atcgtcgatg gctggcacgg cgattcctcg cgcatgtatt
     3421 atgtcggcga ggtgccgcgc aaggcggccc gcctgtgcga ggtaacctat gaatcgctga
     3481 tgcggggcat tgcggcggtc aggcctggcg ccacgaccgg cgatatcggc catgccatcc
     3541 agacctttgc cgaggccgag cgctgctccg tcgtgcgcga tttctgtggc cacggcctcg
     3601 gtgaactgtt tcatgacgaa ccgaacatcc tgcatttcgg ccgccccggc gaagggccgg
     3661 tgctcaagcc cggcatgctg ttcaccatcg agccgatgat caacctcggc aagccgcagg
     3721 tgaaaatcct ctctgacggc tggacagcgg tgacgaggga ccgctcgctg tccgcccagt
     3781 ttgaacacac catcggcgtc accgagaccg gctgcgagat cttcaccgta tcgccgggcg
     3841 ggctgcatca tccgccctat gcaaaggcgg gcgcatgacc aggggcgatg cggcggaaaa
     3901 cctcaacgcc gggcatcgcg accgtctgcg ccggagtttc cgcgcggcag gcgccgaggc
     3961 cctgccggat tacgagttgc tggaactgct gctgttccgc tcgatcccgc ggcgcgacgt
     4021 caagccgatc gccaagctgc tgatcaggcg tttcggctca ttcgccgaag tgcttggtgc
     4081 gccactcgaa cgtctgacgg aagtggaggg cgtcggcgcg gcaaccgcgc tcgatctgaa
     4141 gatcatcgcg gccgccagcc agcgcatggc gcgcggcgac atttccaagc gcacgacgct
     4201 ctcctcctgg agcgcgctga tcgactattg ccgcgtcgcc atggcttttt cagaacggga
     4261 ggaacttcgc atcctttttc tcgacaagaa gaacgggttg atcgccgacg aggtgcagca
     4321 gaccggcacc gtcgatcaca cgccggtcta tccccgcgaa gtcctgcgcc gcgcccttga
     4381 actgtcggca accgccatca tcctggtcca caatcaccct tccggcgatc ccacaccctc
     4441 gacgtccgac atccgcatga cccaggacgt ggtggcgatc gccaagccgc tcggcatagc
     4501 agtgcatgat cacatcatca tcgggcgcaa tggtcatgcc agctttcgcg ggctgggctt
     4561 gctgtaaatc ctcaaagcat tcctccgtga caatcaggca tgggcgctga acaaaggcaa
     4621 agataacgaa cggtaaacct ccttgtgcaa cgttggcaga actaatctac ctgcaatgcc
     4681 ttggattcct gatggacgcc ctaaagaacc ccgggtcccg gaccctctcc acctcgcttt
     4741 cggtcgtccc gatcctgctg gtttccttcg ccgtcatcgg cagtatgtcc ttcctcgcca
     4801 agacagcctt tgaacgcaac gagcggcagg cgatcctgac cgagcagcgc cgcatcgaca
     4861 tggcgctgga acatgaggcc agcgagtttg ccgaccagat ccagcgcgag gcgctgctgc
     4921 tggctcagcc ggagtccggc aagccgatcg acatggaacg gctctcgacc tatctggcca
     4981 gcgagcaccg gctggatttc gtcgcggtct attcgcgcgc cggcaaattg atttccatcc
     5041 ggtccagccg ccctgagatt ctcgagcgtc gcctgaaagc ctccgatgag ctcatcgccg
     5101 aacgcgagac gctgaagacg ccgttcgccc tgcgccgcat cggcatgagc tcgacattgg
     5161 ttgagccccg ggccatcatt tcaatggcgg ccgtcaatgt cggtgatcag tttcagcttg
     5221 tcgtcgcccg ccgcctcgaa gaccggacgc tgacggcgat cggcaaattg ctgctgctgg
     5281 ccgatctgcg ctttgccgaa accgacctga cctccccggc ttcgccggag actggcatga
     5341 cgctggtgtg gaaccgcatc aacgacggat ccttcaacct gcaaagtctc gccagcatcg
     5401 gcttcatggg catcgccgtg atcggcatct acggattgct ggtctatctg cacatccgcc
     5461 gggtgacgcg ggaactggag cagagcgaag cacaggcgca gcatcaggcc ggccacgacc
     5521 cgctgtcagg cctgccgaac cgcagcctgt tccatcgcgc gctcaacacc gaactggctc
     5581 gcgtcaatcg tgatggcggc ggccttgcag tgatgtatct cgacctcgac aagttcaagg
     5641 aggtcaatga ccagttcggt catgagatcg gcgacaagct gatcatccag gtggcaggcc
     5701 gattgggcca gctcgtgcgc ggcgccgata ccgtggcccg gttcggtggc gatgaattcg
     5761 ccatcatcca ggtgggcgtt cgcaccatcg acgatgtcga gaccctggcc cgccgtgtgc
     5821 tcgacgaatt gcgccggccc ttccgcatcg acgatttcga ggtgaacatc ggctgttcga
     5881 tcggcgtgtc cgttgcccct gaaaacggca atgattccgt ccagctcatg cgctatgcgg
     5941 atatcgcgct gtatcgtgcc aagaacgagg gccgcaaccg ctactcgttc ttcgagcacc
     6001 agatgaacga gacgatgcgc ctcaagaaga tcatcgagga cgacctgcga gccgcgatcg
     6061 ataacgaacg tctggttctc cattatcagc cgatcgtgac ggccgacggc agccagatca
     6121 tcgggctcga agcgctggtg cgctggaatc atcccgagtc ggggctcatt ccgccgaatg
     6181 agttcatctc gattgccgag gagcgtggcc tgatctcggc gcttggcgaa tgggttctgc
     6241 gccgcgcctg tctcgacacc aagcattggc cgggcgtatc tgtttcggtc aacgtctcgc
     6301 ccgtgcagtt ccgccagcgc gatttcgttg ccaccgtcgc ccgcattctt gcggagaccg
     6361 ggttcgatcc gcatcggctg gaactggaac tgaccgaggg cgtcgtcgtc gaggacgccg
     6421 atcaggccga ggccgcgatc atggatctgc gcgccatggg cgtccgcatc gctcttgacg
     6481 atttcggcgt cggctattcc agcctgatct atctgcgccg ctttgccttc gaccggatca
     6541 agattgacaa gtccttcctg gaaagcatgg aaggaacagg cgaatccgct atcctcatcc
     6601 attccatcgt gcatctaggc cgggccctcg ggctggaagt caccgccgaa ggtgtcgaga
     6661 ccgaggaaca acaacgcttc ctgcaggcgg tcggctgcca tcatctgcag ggctattact
     6721 tcgcccggcc ctgccctgct gcggaaatcg ccggcgttct cgaaaaggcg caggccatgc
     6781 ccaacgaggg ccgcctggcc gataagccga accctgcctc accccgcgcc gccgcctgac
     6841 atcgggtcaa ggctttgtct cataccaagg gtcatgacaa ttgacccttg gttcctgttt
     6901 tgaattttca tttccctaaa aaaagtaatg aaaattcaaa atacgtcatc ggtcacttct
     6961 cgtgaccgat ggtatcagaa gaacgccgcc gccccctgaa acagggcgta gatcatggcg
     7021 gatgtcaccg ccagcgcgac cggcgtggcc accaggacac cgaacaccat cagcagtcca
     7081 tcgcgctggg tgaaggccag gccgaggatg gtcaccgcga ttccggccgg aatgccgccg
     7141 aaaaccggga ttggcgccat cagggcgacg cccagcgcca gcccggccag gccaaccgcc
     7201 cgccgcatca gatcgcccga cagcaaagga aagcggggct ttgccagtcg ctccatccag
     7261 cgtagcggtc cggtggcctt ttccaccacc cggttcagca tggcccggtc gatctgacgg
     7321 tttccgagcc atcccggcag aagcaattgt gacatgccca gcagcatttg cagcgccacg
     7381 accatcacca ccacgccaca cagcacaggg agccccggcg gcatcggcag gcaattgggc
     7441 aacccgaaca tcaggaacag cagtccgaag gcccggttgc cgaacccggc gacaagctca
     7501 ttgagcgaca ccttcgcctc gctgctgctg ccggcggttc gggcaatacc ggtcaggatc
     7561 gttgacaggc tttgagcggc gacaggatca gacacagaca ggcaactcca gagcggaacg
     7621 ccttttacga ggctacggag aaattgccag cggttttgtt tgcttgggtt agcggcaatg
     7681 tccaccgccg cgctgcgtca caatgcgcaa atcgcaacca agaggcgtat ccctgcgccg
     7741 acgcgcgcag gtgaccgtag cttttccatg actgtcgaag ccaaatgggc cgatggggct
     7801 tggcgtagga acagggctga cggggcggcg gcttgcaaat ctgtttgcag gaatgcaagc
     7861 ctatgtcggt gcctcgatca tggccatgcc tgcgctggct ctcgactgga ctgatgcgct
     7921 gagctgggat gcggtgtttg cgaccgccga tttcgacaac aggacggcct tcgtcgccca
     7981 gggtggcaag aaacagcttt cgatgccgga cgaaggggca aagatgacgc tggccttcgt
     8041 gtccggcgtc tcgctcagcg acattgcccg cgtgcgtcgc ggcaaactgt cggtggtcga
     8101 gattgaccgc tattcacgcc tgctgctggg cctcgaacga tcggcaggcc cggtctatac
     8161 ggcggtcgcg atcggcccgt cgctggccct ggaaaagccg gctgtccggc cggggcgcca
     8221 gcgctttggc gtcgccctcg atgcacaggt ctggctgcgg ccggatacgg caacctactg
     8281 ggccttcgcg ctgaccggcg acacggccgc ccggacgctg tgggcccgcg gtcgccttgg
     8341 ctatcgaccg gagggatggg tgatggcggc aggccccgaa ctgggcgccg cggccgacgc
     8401 gcaatcgggc aagacccggg ttggcctgca tctcggggag atcaccctcg gccccttccg
     8461 cttcgatgtc gccactggcc gtcagtggga tacgcgaagg cgcgccggct cctatgcaac
     8521 gctggcggtt gtcggacggt attgattggg tagtaaaaag gcctgctggc tttgtcggga
     8581 caggaagatc gccttaccgc ttcaggcgcc cgaacgcctc gggcgtgtcg aaatcgcggg
     8641 caattccggc atcctcgacc ggcacctcga tcacatccgc gcccgccgcc tcgatcagct
     8701 tgcgcgcacc ctgatcccct gtcagtccgg cgatccggcc gaacagcgcg cggccgatca
     8761 gcaccggatt accgcgctcg ccgaccagcg tcggcaccac agcctcgacg ccgggatgtt
     8821 caccgaacac gcccgccagc cgttgcagga ctgccggcgc aacaagcggc atgtcggcaa
     8881 gcatcaccag cacgccggca acatcctcag gctgggcggc cagtcccgcc cgcagtgagg
     8941 tagccatgcc ctggacaaaa tcagcgttgg cgacaaagcg caccggcagc cccgccagtg
     9001 ccgccatcac ccgatccgcc tcatggccgg tgaccacgct gaccgggccg acgccggccg
     9061 ccagcgcaac ctccacactc cgccgcacca tcgcaacgcc atcgaccgga agcagcagct
     9121 tgttggcccc ttccatgcgc gtcgagcgcc cggcggccag cacgatgacg ccgagcgagg
     9181 cttgcgcagg aatgaccgtc ggcgcggcag ggctgcgagg ctcgcgcagg tgcgggcggc
     9241 tggcgatctc ggtcagcaac ccgccaaccc ccatcgcctg aatatcgaag cgcgtcacat
     9301 cgaagccggc cagcatccgc tgcaacaccc agtcaaagcc gttctccttg gggctgcgcg
     9361 cgcagccggg cgcgccgatg acccgcgcat cgccgatccg cccgaccagc aggagattgc
     9421 ccggatcgac cggcatgccg aaatgatcca cccggccacc cgcctggcgg atcgccgcag
     9481 gaatgacatc ggccaaatcg gtaatggcag aggcgccaaa gacgatgacg acatcattgg
     9541 cggccgcctg tctggccaat gcatcggcca aagcgttcat ctcatgcggc acacgctgat
     9601 cggcgctgat ggttgtcccg gcagccgcca gccttgccgc tgtcacggcg gcggtcttgt
     9661 cgatgacgct ggccttgagg ccgggcaagg ttgtggagat gagcgcaacc cggcctggtc
     9721 ggaacgccgt gactgaaagc gccccgttcc ccgacccagc agcgaggatc gccccgttca
     9781 ccagcgcatg gtccactgca aacgggatga tcttgacggt cgcgaccatt tccccggcgc
     9841 gcacggcacg gaaatccggc aacgtcgcca cggtaatggc cccgtcgatg ccgttgagcg
     9901 catggatgac ctcggcattg acgcggaaaa cgcccgccgc ttcggccgtc aggttggcgc
     9961 ggccggtgaa tggccgctcg accgcaacgc cgccgcccgc cagcgtctcg gcgatggcca
    10021 gcgcggccga atcctcatcg acatccccct cttccagacg cgcaatgacg acctcgcgga
    10081 cgccggcctt ttccagcagc agcacgtcat tgtccctgag ccgcgtgccc ttgcgcagaa
    10141 ccacgccacc ctgccgcacg gcatgggcca gaatgccgcc gaccgcagcc gcgacaggaa
    10201 ccgatccgaa tttcatggtg gcatggtccg caaggcgccg atgatctcgg caaggatgga
    10261 gacggcgatt tccgccgggc tgatggcgcc gatgtcgagc ccgatcggcg cgtgaatccg
    10321 ggcagaggcc gcctcgtcgc aaccggccgc cgccagccgc gccagccgcc ccgcatgggt
    10381 tttgcggctg cccagcgcac cgatatagaa acacccggct gccagcgcgg cgaccaggcc
    10441 cggatcatcg atcttcggat catgcgtcag cgccaccagc gccgtatacc ggtcgagccc
    10501 gaggcgcgtc agcgccgcct caggccactc ggtcagaagg gccacatcgg ggaagcgctc
    10561 cggggtggca aaggcggtgc gtggatcgat gatggtcaga tcgaacccga cctgccgcac
    10621 catcggcgcc agcgcctgcg caatatgcac tgcgccgatc accacgatgc gcaccggcgg
    10681 cacctgcacg gtgagaaaca gccttcgccc cgcgaccatc gccattgcag agcggccctg
    10741 ccgcaatgcc ttggctgtcg cttcgcccag ctcggttccg gccagcggca ggctgaccag
    10801 atcggcggcc cggaccagcc ggacctcgcc gctttcggga tcggtgacca agagcgccgg
    10861 tcgccgcgcc gcccgttccg cgttcagtgc ggcaagaacc tccagcttca tgccaccggc
    10921 tccacataga cggcgatcct gccgccgcag gacaggccga cgcgccaggc ggtttcatcg
    10981 gcgacgccga aactgagcat tgccggcttg cccgaggcaa tcacctccag cgcctcggtc
    11041 accaccgcgc cttccacgca gccgcccgac acagatccga ggaaattgcc ctcatggtcg
    11101 atcaccagat gcgagccgac cggacgcggc gccgaacccc aggtctccac caccgtcgcc
    11161 agcgcaacgc cccgcccggc ccgggcccag gcttcggctt tggtcagcaa cgcgtcatcg
    11221 cttgcgagca tggcgaacgc ctcctcatcc gtccagtctg gagcgtttta tgcgccgccg
    11281 caatgcgtca gggcaaaccg gactatccgc cgccctgctc attcgggcag ccggaaccgt
    11341 cgcggatcag ccgctgcttc agccggttcg cccgtcagcg cgcggcagag atcggccatc
    11401 gacgcaagat tgtgcagcga gcggaactcg tcgacatgcg gcagcatcgc ccggataccc
    11461 gccgcccgtg cctcgaaacc gtcatagcgc agcaacgggt tgagccagat cagccgccgg
    11521 cacgagcgac gcagccgatc gagcgcgaac gacagttcag ccgtttcctg ccgttccagc
    11581 ccgtcggtga gcagcagcac gatggcgccc tgcccgagca cccggcgcga ccacagccgg
    11641 ttgaaatcgc tgagcgcccg gccgatccgc gtgcccccgt cccagtcgcg caccatcctc
    11701 gccgtcgccg ccagcgccag atccgggtcg cgatgggcgg cgagcgcgcg gctgatgttg
    11761 gtcagttcgg tggcaaagac aaagccatgc acttttcgat ctgcccggcc aagcgcatgc
    11821 agaaaatgca gaaacacctg cgcatagcgg ctcatcgagc cggagatgtc agcaagggcg
    11881 acaatcggcg ggtgcaccgt cagcggcgcg cggaatttca gcgcgatgcc accggccccg
    11941 ctgcgcaacg tcgccctgag cgaagctctg ggatcgaccc tgagcccatt gggatcggcc
    12001 cgcatgcgcc gcgtcaccac ccgatcgagc ggcagcacca gccgggcaac ggcgcgcttg
    12061 gcctcctcga tctcggcggc gctcatctgc tcgaaatcgc gccgtttgaa cacttcaagg
    12121 tcggacacgg tcaggcgcgc gtcgagcatc ttctgctgct cgacctcctg cggcgcgcgg
    12181 ttgcggtcat cgtagagcgc ttcggagacg cggcgcagcg cctcctgccg cggcttgtcc
    12241 ggctcgcccg gcgccaccgg catcatcgcc gccatcatcc gttccatcag cgagcgcttg
    12301 cgccagaaaa tctggaacgc ctgatcaaac accgccatat gctcgcgctt cttgacgaac
    12361 actgcctgca aggtggcgtg gaaatccgcc cggtgaccga ttccggccgc ctccaccgcc
    12421 gccaccgcat ccagcaccgc gccggggccg accggcaaac cggcagaccg cagggccctg
    12481 ccgaaatagg cgatgttttc ggccagcctg ccggtgccgg cttcgaccgc accggcctgc
    12541 atcatcgggc ctcggccctg gcgcggtcga tgagatcctt ggccttcgtg ccctggatct
    12601 tggcgatgtc gtcctggtat ttgagcagca cccccagcgt atccgacacc agcgccggat
    12661 cgagcgccac cgcatcgagt tcgaccagcg cgctcgccca atcaagggtt tcggcgacgc
    12721 ccggcgcctt gaacaggtcc tccct
//
LOCUS       DAOMXG010000058        12634 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_2714_length_12634_cov_8.898482, whole genome shotgun sequence.
ACCESSION   DAOMXG010000058 DAOMXG010000000
VERSION     DAOMXG010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 12634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12634
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2714_length_12634_cov_8.898482"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..1131
                     /locus_tag="PLQ11_05670"
     CDS             130..1131
                     /locus_tag="PLQ11_05670"
                     /inference="COORDINATES: protein motif:HMM:NF013838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliG C-terminal domain-containing protein"
                     /protein_id="HRK24424.1"
                     /translation="MRPANGAERAALVLLALGETHGAPIWAELDDEEAALLSRTIARL
                     GPVDRAFAEAALDLFRTELDSSAGLTGTPRRAEELVSKVLPEPRAAPLLEQLRRPNGL
                     DLWEKLATLSDQVVADYLSLEHPQTVSVILWRMNPNQSARVLARLPDDLAVDCLDRMT
                     RIDAIEPAVVSAIEATLTANLVEPHSGDAPPEPTTRIADIFNVIDKRRAEQLLSGWRG
                     ADAATADRVREQMFTFEDFRKLGPATMQTLMKGLDKDLLALALKASPPETRSFFMDQL
                     SARAARMLDDQISSLGAVRLREAQAAQFKVIAFARELEAAGELSLRIAESASEEMVE"
     gene            1128..1748
                     /locus_tag="PLQ11_05675"
     CDS             1128..1748
                     /locus_tag="PLQ11_05675"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HRK24425.1"
                     /translation="MSALPRFPFEADFSAPPSPPDHAEAPVDEGTAREAAGYARGYDD
                     GAASAMAGIEARLAEAIDRLGENLEGVVAAANERIAALEGEATRLALALARKFAARAL
                     LLDPLGGVEEAFISLIGDLQGEREVSLHLSPELVALAETRLGDLCRARLAPFRLRLMP
                     DAALAAGDARIEWSLGGVVLDHAATDARFTGIVERFLPSPTDEAAS"
     gene            1745..2047
                     /gene="fliN"
                     /locus_tag="PLQ11_05680"
     CDS             1745..2047
                     /gene="fliN"
                     /locus_tag="PLQ11_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HRK24426.1"
                     /translation="MTDTPAPQLPDGVTHDRRKATAPGDLLRLTELPVRVTAVVGRAD
                     IDIAALLALGPGAVLELDRKIGEPVDILVNERLVARGEIVMVDDRLGVTMTELASG"
     gene            2056..3420
                     /locus_tag="PLQ11_05685"
     CDS             2056..3420
                     /locus_tag="PLQ11_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019904649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRK24427.1"
                     /translation="MRLLIVGMLRSELVTAAKIARDRGAQVAQVDGCEQALTHLRAGR
                     GADLIFADVTGDIRGLIDSLRREHLNVPVVACGISTDRTVAVAAIHAGAREFVPLPPD
                     PELIAAIIEAVSHDPDELIVRDPAMARVVELARQIARSDASVLITGETGVGKEVMARF
                     LHARSRRAGRPFVSVNCAAIPEGLLESELFGHEKGAFTGAIARRVGKFEEASGGTLLL
                     DEISEMDLKLQAKLLRALQERVIDRLGGGKPVPVDIRVLATSNRNLAETVRTGQFRED
                     LYYRLNVVNLRIPPLRERPADILELAGHFIRRYAEAAGLPPRTLSSAAKRLLLASPWR
                     GNVRELENALHRAVLLAGGPEIDAEAIVMPDGTAAYATDADPARRAAERAVVATRALV
                     GRSVAEVEQTLILDTLDHCLGNRTHAARILGISIRTLRNKLNDYEAAGLLVPEPGSTR
                     IVTA"
     gene            3548..5656
                     /gene="flhA"
                     /locus_tag="PLQ11_05690"
     CDS             3548..5656
                     /gene="flhA"
                     /locus_tag="PLQ11_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HRK24428.1"
                     /translation="MTDAPLTTSSPQRISLPRIGDLGKVLARGDLALALGVLGILVVL
                     IVPLPPLLLDLLLAVSIIFSVLILMTALFIQGPLEFTSFPAVLLIVTMLRLALNLAST
                     RLILANGHTGGGAAGHVIEAFGAFVIGGNFAIGIIVFIILVIVNFVVITKGSTRIAEV
                     AARFTLDAMPGKQMAIDADLSSGLITQEQARTRRRELEDESAFFGAMDGASKFVRGDA
                     IAGIMITFINVLGGMAIGILQQGMSAGDAARSYTLLTVGDGLVSQFPALIVSTAAGLL
                     VSKAGVTGAADKALMKQLTFYPRALGLSAAVMLVIAVLPGMPMLPFLLLALGAGALAR
                     KAEQVNRQAQAGTAAEAAPPAEDSDNASAALRMDELRIEIGYGLLPLARGPDGEDRLS
                     SQIRALRRAMATEHGFLMPAVRMIDNVQLEPNRYVIYLKEVQAGEGVVFPGQFMAMNP
                     SGAAIDLPGQTMLEPAFGMQVTWIDAPLREEAQFRGLTVVDPGSVITTHLTEIIRANM
                     PDLLSHAETQKLLRELPKDHADLVKELIPQSLSATALQRVLQALLAERVSIRDLPTII
                     EAIAEVAGSLRDPRAIAEQVRTRLARQICAQHASADATLTILPLSAHWEDAFATALVG
                     EGEIRQLAIAPSKLHEFIQVLKVRLEDAAREGQMPVLVASAAIRPYLRMVIERFRPQT
                     VVLSQAEIHSSIRLKSLPML"
     gene            complement(5657..6733)
                     /locus_tag="PLQ11_05695"
     CDS             complement(5657..6733)
                     /locus_tag="PLQ11_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016920504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HRK24429.1"
                     /translation="MKRLKEFIWPIIGLVAVVLSVRVLYQKLYDEAAINPRVSAALEK
                     AGWWDSLKIIARVIGQKLAAIPPEGYLLAFLSTLAAYAALAWYDRIALLHLRKEKGIS
                     WLFISACSFTTYALSHNIGASVVSGGAVRYRAYTSKGLTGAEVAVLVALCSFTFAYGT
                     LILTGLVLVGEPHILTPLLKSPMLRNTPVAGLSLTTIRIIGLVILGLCALYVLFSWMK
                     LKPLTIRGFTLEYPRMEVVVRQLIAAPLELIGAAGIIYFALPDASNPGFFVVLGAFLL
                     SFSAGLLAQVPGGVGVMEAVFIAVMPGVPASSVFAALLVWRLFYLLLPLALSIPIILA
                     FEEKRIVETIHHAHEHKNDPPPKG"
     gene            6844..7455
                     /locus_tag="PLQ11_05700"
     CDS             6844..7455
                     /locus_tag="PLQ11_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRK24430.1"
                     /translation="MDTTLSCLLDGITTLPRRHVATIKFYLERDLTSGNAPPPLPAPL
                     RLERLRGDQSGRYRSLFATLGTRWLWWSRLMFAPGSLAVLLDRPATEAFAVCRGDRDI
                     GLLELDLAALPVADLAFLGLFDGETGKGAGRALMAWALARMAEAGARRASVNTCTFDS
                     PHALGFYRASGFELVRQAVEIVPDPRLSGLLPATAAPHVPILA"
     gene            complement(7521..7940)
                     /gene="fliJ"
                     /locus_tag="PLQ11_05705"
     CDS             complement(7521..7940)
                     /gene="fliJ"
                     /locus_tag="PLQ11_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HRK24431.1"
                     /translation="MKSREALLKFKRFQVEERRRRVSQIEAMIAEFGRMAGDLDREIE
                     TEEKRAGISDPAHFAYPTYAKAARARRDNLLHSAGELKDQLEDAKSALEVAFEDLKKV
                     EVLDDRERAAERAENAMRQQMEADRAAARMRFVAGRA"
     gene            complement(7990..9312)
                     /gene="fliI"
                     /locus_tag="PLQ11_05710"
     CDS             complement(7990..9312)
                     /gene="fliI"
                     /locus_tag="PLQ11_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HRK24432.1"
                     /translation="MTAATLLSLVEDEQPYLVFGRVQGVRGLLVEIGGPLSHMDIGAR
                     VVIETGEAASIGCEVIGFDRDAALAMPFGHLAGVRRGCRALLQSRRGGIRPSGAWLGR
                     VIDAFGEPLDGGPLLTPGPRSYPFRAQPPAAHRRRRLGEPLDLGIRALNSFLTMRAGQ
                     RVGLFAGSGVGKSVLMSMLARNARADVFVIGLIGERGREVVEFIQDDLGDEGLRRAVV
                     VVATSDESALTRRQAAYTTMSVAEYFRDQGQSVVLMMDSVTRFAMAQRDIGLALGEPP
                     TTKGYTPTVFAELPSLLERAGPGEGAGTITAFFTVLVDGDDHNEPVADAARGILDGHV
                     VLDRAIAQRGRFPAINILKSVSRTMPGVADPVFVPTLAEARRLMATYNDMEDLIRLGA
                     YRPGTSPDVDRAIRAHEPLEAFLQQGKEESTSLGESYLRLADIVNGLG"
     gene            9732..10433
                     /locus_tag="PLQ11_05715"
     CDS             9732..10433
                     /locus_tag="PLQ11_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRK24433.1"
                     /translation="MRVLLIEDDSATAQSIELMLKSENFNIYTTDLGEEGVDLGKLYD
                     YDIILLDLNLPDMSGYEVLRSLRVAKVKTPILILSGLAGIEDKVKGLGFGADDYLTKP
                     FHKDELVARIHAIVRRSKGHAQSVIQTGDLIVNLDQKTVEVSGNRVHLTGKEYQMLEL
                     LSLRRGTTLTKEMFLNHLYGGMDEPELKIIDVFICKLRKKLANASEGRNYIETVWGRG
                     YVLREPLDTDERIPA"
     gene            complement(10548..11648)
                     /gene="cheB"
                     /locus_tag="PLQ11_05720"
     CDS             complement(10548..11648)
                     /gene="cheB"
                     /locus_tag="PLQ11_05720"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HRK24434.1"
                     /translation="MGGPSFDAVQPRIRVALVDDQVVVRGLFSRWLAEVEDFEIVGVH
                     RDGAQAVRQIGLARPDVVVLDIEMPEMDGLTALPLLLEASPDSRVLIVSGLSLRGADI
                     TLRCMLRGATDFLPKPGSMREVTTSEEFRSDLIARIRAIGRQRRAVPPVTMPRLPPAA
                     LPAAAGGRTLSGPDLLIVGASTGGPAAVTHLLNGLKPVLHRLAVVVVQHMPQPFTLLF
                     ADHLRRHSGLEAVEIGGGEILRRGCVHVCRGGQSLRIVRSAEGLGVVRDLLPEAECRG
                     IPSIDLVMTSAARACGAGVAGVILTGMGDDGAVGARAIAAAGGVVLAQDEASSIVWSM
                     PAAAIETGLVQAVAPVGDLAVMLVAMATPRSA"
     gene            complement(11671..12036)
                     /locus_tag="PLQ11_05725"
     CDS             complement(11671..12036)
                     /locus_tag="PLQ11_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRK24435.1"
                     /translation="MTYCLVVDDSSVIRKVARRILEGLSFEVSEAEDGQQALDACLHR
                     MPDAILLDWSMPEMDGYQVLQRLRELDHGKRPKVVFCTTVNDVSMIAKAIHLGADDYV
                     MKPFDRHILTAKFGQVGLA"
     gene            complement(12052..12534)
                     /locus_tag="PLQ11_05730"
     CDS             complement(12052..12534)
                     /locus_tag="PLQ11_05730"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HRK24436.1"
                     /translation="MSNAAVNRPRPPAPGDRAQVVTLRLDGQLIGLPIEQVLDVFQVV
                     EPQPVPLTAPSVVGLVNLRGRILPVLSLAGLLGLPKKLPGKTRMAVGVPWRGEIYGVE
                     IDDIGDVIEVSLAASEPLPAHLGETWARAARCVHKLERGLMIELDLVALLEQPLTVAA
                     "
BASE COUNT         2317 a         4260 c         3803 g         2254 t
ORIGIN      
        1 ggccgagccg acgccagcgg ccaatggtgc aacgcctgta ccacccgctc ttgagcacct
       61 cggcgcccgc attgccagcc agccgcagga tgccgccgcc attctcaaga cctggctgag
      121 gagcgcagca tgagaccggc caatggcgcc gaacgcgccg cccttgtcct gctggcgctg
      181 ggcgaaaccc atggagcccc gatctgggcc gaactcgacg acgaggaggc ggctctgctg
      241 agccgcacca ttgcccggct tggcccggtt gaccgcgcgt ttgccgaggc ggcgcttgac
      301 ctgttccgca ccgagcttga ctcatccgcc gggctgactg gcacaccccg ccgcgccgag
      361 gaactggtca gcaaggtgct tcccgaaccg cgtgccgctc ccctgctcga acagctgcgc
      421 cggccgaacg gcctggatct gtgggagaag ctcgccaccc tttccgatca ggtggtcgcc
      481 gattacctct cgctcgaaca tccccagacc gtttcggtga tcctctggcg gatgaacccg
      541 aaccagtcgg cccgagtgct cgcccgattg cccgatgatc ttgctgtcga ttgtctcgac
      601 cgcatgaccc gcatcgatgc gatcgagccg gccgtggtca gcgccatcga ggcgacgctg
      661 accgcaaatc tggtcgaacc ccattcgggc gacgccccgc ccgagccgac cacccgcata
      721 gccgacatct tcaatgtcat cgacaagcgc cgtgccgaac agttgctcag cggttggcgt
      781 ggggcggatg ctgccaccgc cgaccgcgtg cgcgaacaga tgttcacctt cgaggatttt
      841 cgcaaactcg gccccgccac catgcagaca ctgatgaagg gcctcgacaa ggacctcctg
      901 gcgcttgcgc tgaaggcttc gccgcctgaa acgcgatcct tcttcatgga ccagctctcg
      961 gcccgcgccg cccgcatgct ggatgaccag atatccagcc ttggcgccgt tcgcctgcgc
     1021 gaggcgcagg ctgcacagtt caaggtgatc gcttttgcgc gcgagctgga agcggccggg
     1081 gaactctccc tgcgcattgc cgagagcgcg tccgaggaga tggtggaatg agcgcgcttc
     1141 cccgctttcc tttcgaggcc gatttttcag cccccccatc gccgccggac catgccgaag
     1201 ccccggtcga tgagggcacc gcccgcgaag cagccggcta tgcccgcggt tatgacgacg
     1261 gggctgccag cgcaatggcg ggcatcgaag cccgcctggc ggaggcaatc gacaggctcg
     1321 gcgaaaacct ggaaggtgtg gtagcggcgg ccaacgaacg gatcgctgcg ctggaaggcg
     1381 aggccacccg gctggcgctg gcgctggccc gaaagtttgc cgcgcgcgcc cttctgctcg
     1441 atcctcttgg cggagtcgag gaagccttca tcagcctcat cggcgacctg cagggcgagc
     1501 gcgaggtctc tttgcacctg tcgcccgagc ttgtggcgct ggctgaaacc cgtctcggcg
     1561 acctctgccg cgccaggctg gctcctttcc gcctgcgtct gatgccggac gcggcactcg
     1621 ccgccggcga tgcacggatt gaatggtcgc tcggcggcgt cgtgctcgac catgccgcga
     1681 ccgacgcgcg cttcaccggg atcgtcgaac gcttccttcc atccccgacc gacgaggccg
     1741 cctcatgaca gacactcccg caccccaact tccggacggc gtcacccatg atcgccgcaa
     1801 agccaccgcg cctggcgatc tgctgcgtct caccgaattg cccgtgcgtg tcacggccgt
     1861 ggtcgggcgg gccgacatcg acatcgcggc gctgctggcg ctgggccccg gggcggtgct
     1921 cgaactcgac cggaaaatcg gcgaaccggt cgacatactt gtcaatgaac gtctggttgc
     1981 acgtggcgag atcgtgatgg tcgacgatcg cctcggcgtc accatgaccg aacttgcttc
     2041 cggttgaggt ctgccatgcg tcttctgatt gttggtatgc tgagaagtga actggtgacc
     2101 gccgccaaga tcgcccgcga tcgcggcgcc caggtggcac aggtcgacgg atgcgagcag
     2161 gcattgacgc atctgcgcgc cggccggggc gccgatctga tctttgccga tgtcaccggt
     2221 gacatccgcg gtttgattga cagcctcagg cgtgaacacc tcaacgtccc ggtcgtcgcc
     2281 tgcggaattt ccaccgaccg caccgtcgcg gttgcggcaa tccacgccgg cgcccgcgaa
     2341 ttcgtgccac tgccgcccga cccggagctg attgccgcca tcatcgaggc cgtcagtcat
     2401 gatcccgacg agttgatcgt ccgtgatccg gcaatggccc gtgtggtcga acttgcccgc
     2461 cagatcgccc gatcggatgc gtcggtgctg attaccggcg aaaccggcgt cggcaaggaa
     2521 gtgatggccc gctttctcca tgcccgctcg cgccgtgccg gccgcccgtt tgtctcggtc
     2581 aactgcgccg cgatccccga aggcctgctg gaaagcgaac tgttcggcca tgagaagggc
     2641 gcctttaccg gagcgattgc ccgccgtgtc ggcaagttcg aggaagcctc cggtggcacc
     2701 ctgctgctcg acgagatctc ggaaatggat ctcaagttgc aggcaaaact cctgcgcgcc
     2761 ctccaggagc gggtgatcga ccggctgggc ggcggcaagc ctgttccggt cgacatccgc
     2821 gtgctggcga catccaaccg caatctcgcc gaaacggtgc gcaccggcca gttccgcgag
     2881 gatctctact accggctgaa tgtggtcaac ctgcgtattc cgccgctgcg cgaacgcccc
     2941 gctgacattc ttgaactcgc cggccatttc atccgccgct acgccgaggc ggccggcctg
     3001 ccaccccgca ccctgtcgag cgcggccaag cgcctgctgc tcgcctcgcc ctggcgcggc
     3061 aatgtgcgcg aactcgaaaa cgccttgcat cgcgccgtgc tgcttgccgg aggccccgag
     3121 atcgacgcgg aagccatcgt catgcccgac ggcaccgccg cctatgccac ggatgccgac
     3181 cccgcccgtc gtgccgcaga acgggcggtg gtcgccaccc gcgcacttgt tggccggtcg
     3241 gtggccgagg tcgagcagac gctgatcctc gacacgctcg accattgtct gggaaaccgc
     3301 acccatgcgg cgcggatcct cggaatatcg atccgcacgc tgaggaacaa gctcaacgat
     3361 tacgaggccg ctggcctgct ggttccggag cccggcagca cgcgcatcgt tacagcctag
     3421 agcactttca gtgaattcac attcattgaa agtgctccag attcttgttt tgtcgcattt
     3481 tcttcacgcg agccggactc cgcttcgctt gaaaatgctc tagtcatcag catactgagc
     3541 gagcaccatg acggatgccc ccctgaccac ctcctcaccg cagcggattt cgctgccgcg
     3601 gatcggcgat ctcggcaagg tcctggcacg cggcgatctg gccctggcgc tcggcgtgct
     3661 cggcattctg gtcgtgctga tcgttccgct gccgccgctg ctgcttgatc ttctgctggc
     3721 ggtctcgatc atcttttccg tgctgatcct gatgaccgcc ctgttcattc agggcccgct
     3781 ggaattcacc tcgtttcctg ccgtcctgct gatcgtcacc atgctgcggc tggcgctcaa
     3841 tctcgcctcg acccggctca tccttgccaa cgggcacacc ggcggcgggg cggccggtca
     3901 tgtcatcgaa gccttcgggg cctttgtcat cggcggcaac ttcgccattg gcatcattgt
     3961 tttcatcatc ctggtgatcg tcaatttcgt cgtgatcacc aagggttcga cccgcatcgc
     4021 cgaggttgcg gcgcggttta cccttgatgc catgccgggc aagcagatgg ccatcgacgc
     4081 cgatctttct tccgggctga tcacccagga acaggcccgc acccggcgca gagagcttga
     4141 agatgaaagc gcttttttcg gcgcgatgga tggcgcctcg aaattcgtgc gcggcgatgc
     4201 cattgccggc atcatgatca ccttcatcaa tgtgctcggc ggcatggcca tcggcattct
     4261 gcaacagggc atgagcgccg gcgatgccgc ccgctcctat accctgctga cggttggcga
     4321 tggcctggtc agccagttcc ccgccctgat cgtgtcaact gctgccggcc ttctggtgtc
     4381 gaaggctggc gttaccggcg ccgcggacaa ggcgctgatg aagcaactga ccttctatcc
     4441 ccgcgcgctc gggctttcgg ccgccgtcat gctcgtcatc gcggttctgc cgggcatgcc
     4501 gatgctgccc ttcctgctgc tggcgcttgg tgccggcgct ctggcccgca aggcagagca
     4561 ggtcaaccgc caggcccaag ccggcactgc cgccgaggcc gcgccgccgg ccgaggacag
     4621 cgacaacgcc tctgccgccc tgcgcatgga tgaattgcgc atcgagatcg gttacgggct
     4681 gctgccgctg gcgcgcggcc cggatggcga ggatcgtctt tccagccaga tccgggcgct
     4741 gcggcgcgca atggccaccg aacatggctt cctgatgcct gccgtccgga tgattgacaa
     4801 cgtgcagctc gagccgaacc gctatgtcat ctatctcaag gaagttcagg cgggcgaagg
     4861 cgtggttttc cccggtcagt tcatggcaat gaacccttcc ggtgccgcta tcgacctgcc
     4921 tggccagaca atgctggagc cggcattcgg catgcaggtg acctggatcg acgccccctt
     4981 gcgtgaggaa gcccagtttc gcgggctgac tgtcgtcgat cccggttcgg tgatcaccac
     5041 gcatctgacc gagatcatcc gcgccaacat gcccgacctg ctcagccatg ccgaaacgca
     5101 gaaacttttg cgtgaattgc ccaaggatca cgccgatctt gtcaaggagc tgatcccgca
     5161 atcgttgtcg gcgaccgcct tgcagcgcgt cctgcaggcg ctgctggccg agcgcgtctc
     5221 gatccgcgat ctgcccacca tcatcgaagc gatcgccgaa gtggccggct cgttgcgcga
     5281 tccgcgcgcc attgccgaac aggtgcgcac gcgccttgcc cgccagatct gcgcccagca
     5341 tgcttctgcc gatgccacgc tgacgatcct gccgctgtcg gcacactggg aggatgcctt
     5401 tgccacggcc ctggtcggcg aaggcgaaat ccgtcagctc gccatcgccc cgtcaaagct
     5461 gcatgagttc attcaggtgc tgaaagtccg gctggaggat gcggcacgcg aagggcagat
     5521 gccggtgctg gtggcgagcg ctgcgatccg cccctatctg cgcatggtga tcgagcgctt
     5581 ccggccgcaa accgtggttc taagccaggc cgagatccat agctcaatcc ggttgaagag
     5641 cctgccgatg ctgtgactac ccctttggtg gcggatcgtt cttgtgctca tgggcgtgat
     5701 ggatggtctc gacgatccgc ttttcctcga acgccaggat gatcgggatc gatagcgcca
     5761 gcggcagcag cagatagaac aggcgccata ccagcagggc ggcgaacacg ctggaggctg
     5821 gaacacccgg catcacggca atgaacaccg cctccataac cccgacacca cccggaacct
     5881 gcgccagcaa tccggccgaa aaggacagaa ggaacgcccc cagcaccacg aaaaatcctg
     5941 gattgctcgc atcggggaga gcgaaataga tgattcccgc cgccccgatc agctccagcg
     6001 gcgcagcgat caactgccgg accaccacct ccattcgcgg atactcaagc gtgaaaccgc
     6061 ggatcgtcag cggcttcagc ttcatccagg agaacagcac atagagtgcg cacaggccaa
     6121 gaatgaccag gccgatgatg cggatcgtcg tcagactcaa cccggcaacc ggcgtattgc
     6181 gcaacatcgg cgatttgagc agcggggtca ggatatgcgg ctccccaacc agcaccagcc
     6241 cggtcaggat cagcgttcca taggcgaagg tgaaagagca cagggccacc agaacagcca
     6301 cttccgcccc cgtcagtccc ttggacgtat aggcacggta gcgcaccgct ccgcccgaga
     6361 ccaccgaggc gccgatgttg tgcgacagcg cataggtggt gaacgaacag gccgagatga
     6421 acagccagga aatgcccttt tccttgcgca ggtgcagcaa tgcgatgcgg tcataccagg
     6481 caagcgccgc ataggcggca agcgtcgaca ggaacgccag cagatagccc tcgggcggga
     6541 tcgctgccag cttttggccg atgacccggg caatgatctt gaggctatcc caccagccgg
     6601 ccttctccag cgcggccgaa acccgcggat tgatcgccgc ctcgtcatag agcttctggt
     6661 agagcacgcg caccgaaagc acgacagcga caaggccgat gatcggccag atgaactctt
     6721 tcaaccgctt catgctgcgc agctttttcc cgtcgtgaac cccgctgctt cctgcacaag
     6781 ccgcatcggc aaagcaagcg gtaaacgtgt gacggcggcg gcaaagcact ctagaaggag
     6841 gctatggaca ccacattgtc atgcctcctc gacgggatca ccacactgcc gcgccgccat
     6901 gtcgcgacga taaaattcta tctcgagcgc gatctgacat ccggcaatgc gccaccgccc
     6961 ctgcctgctc cgctgcgcct cgagcgcctg cgcggcgacc agagcggccg ttatcgttcg
     7021 ctgtttgcga cactcggcac ccgctggctg tggtggtcac ggctgatgtt tgcgcccggg
     7081 tccctggccg tactgctcga ccgaccggcg accgaggcct ttgccgtctg tcgcggggat
     7141 cgtgatatcg gcctgctgga acttgatctt gccgccctgc cggtcgctga tcttgctttt
     7201 cttggcctgt tcgacggcga gaccggcaaa ggagccggtc gtgccctgat ggcctgggca
     7261 ctggcccgca tggccgaggc gggcgcccgc cgggccagcg tcaacacctg cacgttcgat
     7321 tccccgcatg ccctggggtt ttatcgcgct tcgggctttg aactggtccg gcaggcggtc
     7381 gagatcgttc cggatcccag gttatccggc ctgctgccgg cgactgcggc accgcatgtg
     7441 ccgatccttg cctgaaacgc agcacggccg ggcttttggc ccggccgtgc aggatccttg
     7501 ttggcagaac gccgggcggg tcaagcccgt ccggcaacga agcgcatccg ggctgcggcc
     7561 cgatcagctt ccatctgctg gcgcatggcg ttttcggccc gttcggctgc ccgttcgcga
     7621 tcgtcgagca cctcgacctt cttgaggtcc tcgaaggcca cttcgagggc cgatttggca
     7681 tcctcaagct ggtccttgag ttcgcccgcc gaatgcagaa ggttgtcgcg acgggcgcgg
     7741 gcagccttgg cataggtcgg ataggcgaaa tgggcgggat cagagatacc ggcccgtttt
     7801 tcctcggttt cgatttcacg gtcgagatcg ccggccatgc ggccgaattc ggcgatcatc
     7861 gcctcgatct gcgaaacgcg gcggcgacgc tcctccacct ggaagcgttt gaatttcaat
     7921 aaagcttcgc gcgacttcat ggccccttac tctactctac gctacaccga gactggccat
     7981 atcgaggccc tatcccagcc cgttaacaat gtccgcgaga cgaagataac tctcgcccaa
     8041 ggaggttgat tcttccttac cttgttgaag aaaagcttcg agaggttcat gagcacggat
     8101 tgcccggtcc acatcggggc tggttccagg cctgtaggcg cccagacgga tcagatcttc
     8161 catgtcatta taagtagcca tcaggcgccg tgcttcggca agcgtcggca caaacacggg
     8221 atcggccacg cccggcatgg ttcgtgacac cgatttcagg atattgatcg ccggaaagcg
     8281 gccgcgctgg gcgattgccc gatccagcac gacatgaccg tcgagaatgc cgcgtgccgc
     8341 atccgcgacc ggttcattgt gatcgtcccc atcaaccaga acggtaaaaa aggcggtgat
     8401 cgtgcccgcc ccctcgcctg gccctgcccg ctccagcagg ctgggcagct cggcaaacac
     8461 cgtcggcgta tagcccttgg tcgtcggggg ttcccccagc gccagcccga tatcgcgctg
     8521 ggccatggca aagcgggtca cgctgtccat catcagcaca accgactgcc cctgatcccg
     8581 gaaatattcg gcgacactca tggtcgtata agcagcctgg cggcgggtca gtgccgattc
     8641 atcggaggtc gccaccacga ccacggcacg ccgcaagcct tcgtcgccga gatcgtcctg
     8701 tatgaactcg acgacttcgc gaccacgctc gccgatcagc ccgatgacga acacatcggc
     8761 cctggcattg cgcgccagca tcgacatcaa caccgacttg ccgacgccgg acccggcaaa
     8821 cagcccgacg cgctggcccg cgcgcatggt cagaaaactg ttgagggccc ggatgccaag
     8881 atcgagcggc tcgcccagac ggcgccggcg atgggccgcc ggcggttgag cccggaaggg
     8941 atagcttcgc ggtcccggcg tcagcagcgg accgccatcc agcggctcgc cgaacgcatc
     9001 gataacccgg cccagccagg cgccggacgg gcgaatcccg cctcttcgtg attgcagcag
     9061 cgcacggcag ccccgtctga cgccggccag atgaccgaac ggcatggcca gcgcagcatc
     9121 gcgatcgaag ccgatgacct cgcaaccgat cgaggccgcc tctccggttt cgatcacgac
     9181 gcgggcgccg atgtccatat gggacagggg accaccgatc tcgaccagca gaccccgcac
     9241 gccttgaacg cgaccgaaca ccagataggg ctgttcatcc tcgacgagac taagaagcgt
     9301 cgcagccgtc acgacccgtc tctcgcgttg attcgcggtc aatttaaccc gttcttgacc
     9361 ttttcgcgaa aggatttggc aaccggccaa ggttgacgcg acggcccggt ctggtcccgt
     9421 tttgaaccgg ttgccggcct gcaagaccca aaccgatgag tcgaaagagt cgcttaatga
     9481 cttttcttaa tgtgctgcat taattgtttt tctcctaaaa aatcaaagct ctatggccac
     9541 ttacaggctg ttttacgact cacaacggtt accgcttgcc aacaggaatc agcttttgtt
     9601 aaccacttgt cggtaagact tgatcccgcg agcttcacga aggtgaattt tgtcgaatcg
     9661 cggcggacag cagtggcgtt ccgggcgtga ttgggcgggg ttcggataac tatggcttgg
     9721 ggacctgacg aatgcgtgtt ctcctgatcg aagacgacag cgccacggcg cagagcatcg
     9781 aactgatgct caagtctgaa aatttcaaca tctacaccac cgacctcggc gaggagggtg
     9841 tggatcttgg caaactctat gactacgaca tcatcctgct cgatctgaac ctgcctgaca
     9901 tgtcgggcta cgaggttctg cgcagcctgc gggtggccaa ggtcaaaacg ccgatcctga
     9961 tcctgtcggg tctggccggc atcgaggaca aggtgaaggg cctcggcttc ggcgccgacg
    10021 actatctcac caaacctttc cataaggatg agctggtcgc tcgcatccac gcgattgtcc
    10081 gccgctccaa gggccatgcc cagtcggtga tccagaccgg cgatctgatc gtcaatctcg
    10141 atcagaaaac cgttgaggtc tccggcaacc gggtgcacct caccggcaag gaataccaga
    10201 tgctggagct gctttccctg cgtcgcggca ccacgctgac caaggaaatg ttcctgaacc
    10261 acctctacgg tggcatggac gagccggaac tgaagatcat cgacgtcttc atctgcaagc
    10321 tgcgcaagaa gctcgccaac gcctccgaag gccgcaatta catcgaaaca gtctggggcc
    10381 gcggctatgt gctgcgcgaa ccgctcgaca ccgacgagcg catcccggcc tgaccggctt
    10441 ttttacgaag ctccatctcc ccacaactgg cccgcgctga aacgcgggcc ttttgctttg
    10501 gttgtgacca ttcgccaggg gaatggcgtc acccccgaaa aagtgcgtca ggcggaccgc
    10561 ggcgtggcca tggccaccag catcaccgcc agatcgccga caggtgcgac cgcctgcaca
    10621 agtcccgttt cgatcgctgc agccggcatg ctccagacaa tgctgctggc ttcgtcctgc
    10681 gcaagaacca cccctcccgc cgcggcaatg gcccgtgcgc cgacagcgcc atcgtcgccc
    10741 attccggtca ggatcacacc ggccacaccg gccccgcagg cccgcgccgc gctggtcatg
    10801 accagatcga tcgaggggat tccgcggcac tctgcctccg gcaacagatc acgcaccacg
    10861 ccgaggcctt ccgcgctacg tacgatccgc agcgactggc cacctcggca gacatggacg
    10921 caaccccgtc gcaagatttc tccgccaccg atttcgacag cctcaaggcc ggaatgacgc
    10981 ctgaggtggt cggcgaacag cagggtgaac ggctgcggca tatgctggac cacgacaacc
    11041 gccagccgat gcagcaccgg cttgagacca ttgagcaaat gcgtgacggc agccggtcct
    11101 ccggtggacg ctccgacgat cagcaggtcc ggaccagaga gggttcttcc ccccgctgct
    11161 gccggcaggg ctgccggcgg caatcgaggc attgtcacgg gcggaaccgc ccggcgctgg
    11221 cgaccgatcg cccggatacg tgcgatgaga tcggatcgaa actcttcgga ggtcgtgacc
    11281 tcgcgcatgc tgcccggctt gggcagaaag tccgtagcgc cccgcaacat gcagcgcagt
    11341 gtgatatcgg cgccgcgcag actgagcccg gagacgatca gcacgcgact gtcgggcgaa
    11401 gcctccagca acaggggcaa cgcggtcagg ccgtccatct ccggcatttc aatgtcgagg
    11461 acaacgacat cgggccgcgc aagaccgatc tgccgcacgg cctgtgcgcc gtcgcgatgc
    11521 acgccgacga tctcgaaatc ctcgacctcg gccagccagc gcgaaaaaag gccgcgcacc
    11581 accacctgat cgtccacgag ggcgacccgg atgcgcggct gtaccgcgtc gaacgaaggg
    11641 ccgcccatgg atgcggcggg accgtcaggc tcaggccagt ccgacctggc cgaatttcgc
    11701 tgtcaggata tgccggtcga acggcttcat cacataatcg tcggcgccga gatgaatggc
    11761 cttggcgatc atcgagacat cattgaccgt ggtgcagaac accaccttcg gccgcttgcc
    11821 gtggtcaagt tcgcgcaacc gctgcaacac ctgatagccg tccatctcag gcatgctcca
    11881 gtcgagcagg atggcatccg gcatccggtg gaggcaggcg tcgagcgcct gctggccatc
    11941 ctcggcttcg ctcacctcga acgacaggcc ctccaggatg cgccgggcga ccttgcggat
    12001 cacactcgag tcatcgacga caagacaata ggtcatgata cagtctccgt ttcaggccgc
    12061 gacagtcagc ggctgttcaa gcaaggcaac aagatcaagt tcgatcatca gcccgcgttc
    12121 gagcttgtgc acgcagcggg cagcccgcgc ccaggtctca ccaaggtggg ctggcaaagg
    12181 ttcgctggcc gccagcgaaa cctcgatcac gtcgccaata tcgtcgatct cgacgccata
    12241 gatctctccg cgccatggca cgccgacggc catgcgcgtc ttgccgggca gcttcttcgg
    12301 caggcccagc aggccggcaa gcgacagtac cggcaggatg cgtccgcgca gattgaccag
    12361 accgacgacc gaaggagcgg tcagcgggac cggctgcggc tccacaacct gaaagacatc
    12421 gagcacctgc tcgatcggca gaccgatcag ctgtccatcg agcctcaacg tgacaacctg
    12481 agcccgatca ccgggagcag gcgggcgagg tcggttcacc gcagcgttgc tcatgcggca
    12541 acctcgctga tcggtgccgg ttccgccagc agggccagca gcgcccggcg atcgaagctg
    12601 ccgaccacat cgcagcaccc ggccgcccgg gccg
//
LOCUS       DAOMXG010000059        12530 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_2738_length_12530_cov_10.235591, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000059 DAOMXG010000000
VERSION     DAOMXG010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 12530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12530
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2738_length_12530_cov_10.235591"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..>1633)
                     /locus_tag="PLQ11_05735"
     CDS             complement(57..>1633)
                     /locus_tag="PLQ11_05735"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRK24437.1"
                     /translation="PPRGAEYPVYSGIIRSAKGRLGAFELTVDSFAPPVQGMRGPMRF
                     GPPRNGASSTCDLILDVSGRMPLFSAHELRPGYLRASPGDRAAIERLIAEARDLVGTF
                     DKPRYVAFKPELCAHSRSRITGCTRCLDICPTGAITPDGDSVAIDPGICGGCGGCSAL
                     CPTGAASYALPAPDHAIASLRTLLTSYFAAGGQDPVVLVHDHEHGEEMIHLLAHAGAG
                     LPARVLPLAVNEVTALGLEAIAAAFAYGATALRVLARARPRHDDAPLRATLTMAGTIL
                     SGLGFSDDAASVIATDDADTLGAALRAIGNGAAAIRRASFLPAGTKRQLQWLALRELH
                     AIAPAPKRSIALPAGAPMGRVTVASDDCTLCLACVSACPAEALRSNPERPELKFSEDL
                     CVQCGLCAATCPEKVISLEPRLDFDAIDAGPIVLKQEDPHACERCGKLFGTRSTIARI
                     RDKLAASHWMFTGAHADRMKLIGLCEDCRVIAATEAAIDPYAGAPRPKPRTAEDYRRV
                     SEGIDLDPQGKKDTGV"
     assembly_gap    1634..1643
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1644..2116)
                     /locus_tag="PLQ11_05740"
     CDS             complement(<1644..2116)
                     /locus_tag="PLQ11_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HRK24438.1"
                     /translation="MPLPHHAIILCSCAGRQPRDVEHLSRSLPGSDIVELRDPVGSDR
                     DRLLRLARSVSSLTIACSCQSAAIEDALEEAGIDVPVTFANLNEPAAFSDEAAAATPK
                     MAALLALAGAPAPPFGLYSMESQGITVVYGSDETALEVARRLADDLDLTILLAGTP"
     gene            2212..2661
                     /locus_tag="PLQ11_05745"
     CDS             2212..2661
                     /locus_tag="PLQ11_05745"
                     /inference="COORDINATES: protein motif:HMM:NF028227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate--protein ligase family protein"
                     /protein_id="HRK24439.1"
                     /translation="MGRKVAADVWDERFPPLFEPVRLREHEDAFSVAMARAAESGAGT
                     VFHVGRFDPIEFAVVLEPDMPLAAARRALFVGMNALAATILADCPPERVVEFSYPDGL
                     VFDNGLIGGARIAFQDGRGDRAAVKVNEAGRKGSTRTRFPIGWCFRR"
     gene            2646..3008
                     /locus_tag="PLQ11_05750"
     CDS             2646..3008
                     /locus_tag="PLQ11_05750"
                     /inference="COORDINATES: protein motif:HMM:NF028227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate--protein ligase family protein"
                     /protein_id="HRK24440.1"
                     /translation="MFSAMIRTGSFQELGFALDPSVTSLEEAGYRDIDPRGFAARFCR
                     HLMVELDEWQEAGFKEIGRRYLDRLPKPPGTGMRGIDRNGDLITHPGGGRAAQRQDFL
                     PALAASAWFDPATGAPRA"
     gene            3005..3547
                     /locus_tag="PLQ11_05755"
     CDS             3005..3547
                     /locus_tag="PLQ11_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017482692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6505 family protein"
                     /protein_id="HRK24441.1"
                     /translation="MKLIRTIRLDPSDTFVFTRSAEPGEWAVSGAFVFWNQPLEGLVG
                     KERAAFRSGLLGIASLGWSTLAVVSEATAAERAGAVDQLARQLVARFGAPDLATARAA
                     AEQEIDYAEQLAEPPVGTLIAVRRSDENGDIRERFRTLLPGGDDLHNRAFHFEMSDEE
                     PEERVDLMALDNASNPQGRA"
     gene            3544..4554
                     /locus_tag="PLQ11_05760"
     CDS             3544..4554
                     /locus_tag="PLQ11_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6352 family protein"
                     /protein_id="HRK24442.1"
                     /translation="MSDDFWLASGHHLLDRDGNGGGLLLTDDFLKAYLARPELVPPAE
                     ACPVERGVHAELMASPRHRVATATLDAIADADARDNIRVFLTFRDHLLRHRTLEDAYV
                     GLFRARLTGIPALFVQQLVAVVARNAFDRCADPFVLRAAECLFRPQKVTIHEGALLLA
                     DAEVIDSHEANRKHSPLLTMLGGPAVTELSVLNDSNAASYFHRSDAHDLVLNLFDPAH
                     GRAALGEAARVFIRHLCGIDLVLEPVEQLDGEPQAWFLAFDAEATAIGNAVWRGEMLD
                     PDWAARVLALYRFRLPDDRRIAEVRRGRPGFALAAMTSDRMLNIKPQNLILGLPLNER
                     DG"
     gene            4558..5034
                     /locus_tag="PLQ11_05765"
     CDS             4558..5034
                     /locus_tag="PLQ11_05765"
                     /inference="COORDINATES: protein motif:HMM:NF023177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3305 domain-containing protein"
                     /protein_id="HRK24443.1"
                     /translation="MVEMTIRVGVVAERRPVDHPWVDHVWEAVAVLPDAPAVAPGTIL
                     LDQGGVVRLYAGSADIVLASSDTAQYRDNLMRPPPRLWVVLRERQPEASVEVVAVTAD
                     PMEAEGHAEDSSNIVGALTMDADMAAAVALFVDTHHVEQVFVKRQRKRHRDGEAEA"
     gene            5031..5627
                     /locus_tag="PLQ11_05770"
     CDS             5031..5627
                     /locus_tag="PLQ11_05770"
                     /inference="COORDINATES: protein motif:HMM:NF023176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3306 domain-containing protein"
                     /protein_id="HRK24444.1"
                     /translation="MSRNEGFLSRWSARKLAPPEPAAKPGEAAAEQPSKEEPAQTGEP
                     FDPASLPSIDSLVADSDIRPFLRAGVPEALKTAALRRMWVSDPAIRDYIGPADFQWDF
                     NTPGALAGFGELPQGTDIVRMLSDLGEGHRPESAQGDDGAAKLLQAPADAGDLPAPPA
                     ESATEVITGECPNSLPAPDEEAATKPVRRRRHGAATPV"
     gene            5772..6620
                     /locus_tag="PLQ11_05775"
     CDS             5772..6620
                     /locus_tag="PLQ11_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HRK24445.1"
                     /translation="MRDTGLEQAIRAAGGISSLARALGVAQPSVSNWSRIPAERVLAV
                     EAITAVPRTTLRPDLYPVDAAADAVDPVDQARGQLYLLLANLLLKVPSDAVLADVAHL
                     PQGAPGPIGEALGALAAAAREARAEAVGREHFDLFVGVGRGELVPFASYYRTGFLYER
                     PLVKVREDLKILGLQRSDDLGEPEDGIGFLCEVMAGMALGRFAVPESYARGFFNRHLA
                     PWAERFFADLEAAGAARFYRAVGRLGRSFIGLEREAFALDDDSHVAASIAHGTNSEGK
                     HDHKAC"
     gene            6601..6801
                     /locus_tag="PLQ11_05780"
     CDS             6601..6801
                     /locus_tag="PLQ11_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HRK24446.1"
                     /translation="MTTKRADETLNRRGFLRAGLGTASVAATTVIGAAQPAQAAENDA
                     ERKKARYKETDHVKTFYRTNRY"
     gene            6812..9736
                     /locus_tag="PLQ11_05785"
     CDS             6812..9736
                     /locus_tag="PLQ11_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HRK24447.1"
                     /translation="MLIKRKAGQVRRGASAGAFAAINGAGMDRRAFLRNSGLTAGGLA
                     ALSSLTFAGVQRAEAGPVAADVAITRKRNMCTHCSVGCSVIAEVQNGVWVGQEPAWDS
                     PINRGTHCAKGAASRELVKGERRVKTPMKLVNGQWQRISWDQAIDEIGSKMLEIREKS
                     GPDSVYLLGSAKFTNEAAYLFRKFAAFWGTNSVDHQARICHSTTVAGVANTWGYGAQT
                     NSYNDIRNAKTVIFMGSNAAEAHPVSLQHILTGKETQRANVIVFDPRFTRTAAHSTEF
                     VRFRSGTDIPVIWGMLHHIFKNGWEDKGFIQSRVYGMEMVRKEVEKWTPEEVEKVTGI
                     PGEQLKRVAETFAKQRPSTFIWCMGGTQHTVGTANVRAYCTLLLATGNVGQFGTGANI
                     FRGHTNVQGATDLGLDIGNLPLYYGLAEGAWRHWARVWDVPYDYLLSRFVDKKMMEAP
                     GIPSTRWFDAVTLPKDQVDQKDNVKAMFIMGHGGNTIPRMPDSAKGLEALELLVVVDP
                     HPTTFSVLSNRKNGSYILPACTSFEMDGSRTASNRSIQWGEKVVEPIFESKNDYEIIY
                     RFARKLGFATEMFKNITVNGVQPVPEDILREINKGGLSTGYSGQSPERLKLHMANQGA
                     FDVVSLKAADGPAKGDYYGLPWPCWGKPEHKHPGTHTLYNTNLHVKDGGGTFRARFGV
                     TYEEPQADGSKKTINMLSEGSYSVGSEIKDGYPEFTYGVLKKLGWDIDLRPDEIETIL
                     KIGGNNPDGVGWAVDLSCGIIRVALEHGCIPYGNGKARANAWNLPDPIPVHREPIYTP
                     RPELVAQYPTRPDGKQFRVPNIGLSIQKDAVEKGIAKQFPIILTSGRLVEYEGGGEET
                     RSNPWLAELQQDMFVEINPNDARSRGISDGQWVWVLGPVGGAKAKVKALVTERVGRGV
                     AFMPFHFAGWYQGEDQRPKYPKGADPVVLGESVNIVTTYGYDPVTGMHEGKATLCDIK
                     AV"
     gene            9748..10344
                     /gene="fdh3B"
                     /locus_tag="PLQ11_05790"
     CDS             9748..10344
                     /gene="fdh3B"
                     /locus_tag="PLQ11_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase FDH3 subunit beta"
                     /protein_id="HRK24448.1"
                     /translation="MARMKFLCDSDRCIECNACVTACKNEHEVPWGINRRRVVTLNDG
                     LPGERSVSMACMHCTDAPCAAVCPVNCFYTTADGVVLHSKDLCIGCGYCFYACPFGAP
                     QYPKVGNFGSRGKMDKCTYCAGGPEDDRTKAEYEKYGRNRLAEGKLPLCAEMCSTKAL
                     LAGDGDIIAQIYKERVAKRGYGSGAWGWQVAYKETVAL"
     gene            10415..11425
                     /locus_tag="PLQ11_05795"
     CDS             10415..11425
                     /locus_tag="PLQ11_05795"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HRK24449.1"
                     /translation="MTHPFRWLALLAALVFPLLVSAVPASAQQGQQINPTAQSLKEAE
                     LLKALKGGDAISGRISIPDAKAATLIQPDGRSWQDFKRSTLPMLGALLILGMLALLVL
                     FYAIRGRIRIDSGPSGQFIERFGAIDRFVHWLTASSFIILGLTGLNVTFGRSIVLPIV
                     GPEAFTTLSEFGKMLHNYVGFAFMVGVVLTFLIWVKDNLPHSRDLVWLSHFGGLFSKG
                     DHPPAERFNAGQKGIFWSVVIGGALMSLSGLHLIFPNSVGGGVAEIQFHSMIHGIVAM
                     VMVAIILAHIYIGSVGMEGAKEAMTTGEVDLNWAKEHHSLWVERELKAKGQAIVHPAA
                     AE"
     gene            11499..12020
                     /gene="mobB"
                     /locus_tag="PLQ11_05800"
     CDS             11499..12020
                     /gene="mobB"
                     /locus_tag="PLQ11_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HRK24450.1"
                     /translation="MKIIGITGWSGAGKTSLIVKLIPELRARGLTVSTLKHAHHGFDV
                     DVPGKDSHMHRTAGATEVLVSSGRRFALMHELNGEAEWSLPRLLGKLNPVDLVLVEGF
                     KRYPHPKIEVYRVANGKPMLHPGDASIRAVAADCPLPEAGRTVYHIDDLTGLAEAVLS
                     LSEPAGSVFAELG"
     gene            12020..>12530
                     /locus_tag="PLQ11_05805"
     CDS             12020..>12530
                     /locus_tag="PLQ11_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020094838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdenumtransferase MoeA"
                     /protein_id="HRK24451.1"
                     /translation="MAQLSDDCFAFGGPLMTVDEAIALIGERIQPVAGEDRVGSAEAC
                     GRILAEPVTASVAVPSFDNSAVDGYAVRHADLAAEGVTTLPVSARIAAGCRTLAPLTP
                     GTAARIFTGAPMPEGADTVFMQEDVQEDGGRVTLPPGLKQGANRRFAGEDFKLGAEVL
                     AAGTRLDPRH"
BASE COUNT         2208 a         3852 c         4234 g         2226 t
ORIGIN      
        1 tttgtttttt cgcattttct taacgcgaac cggaatccac ttcgcttgaa aatgccctac
       61 acacctgtgt ccttcttgcc ttgcggatcg aggtcgatgc cctcggagac gcggcgatag
      121 tcttcagcag tgcggggctt ggggcgtggt gcgcccgcgt aggggtcgat cgccgcctct
      181 gtcgcggcga tgacccggca atcctcgcac agaccgatca gtttcatgcg gtcggcgtga
      241 gcgccggtaa acatccagtg actggccgcg agtttgtcgc gaatccgggc aatggtcgat
      301 cgtgtgccaa acagcttgcc gcagcgttcg caggcgtgcg gatcttcctg cttgagcaca
      361 atcgggccgg catcgatggc gtcaaaatcg aggcgcggtt ccagcgaaat gaccttctcc
      421 gggcaggtgg cggcgcaaag tccgcattga acgcagaggt cctcgctgaa tttcagttcc
      481 ggccgttcgg gattcgagcg caaggcttct gccggacagg cggaaacgca ggccagacac
      541 agggtgcagt catcgctggc cacagtcacc cgccccatcg gagcgccggc gggcaaggcg
      601 atggatcgtt tcggtgctgg tgcgatggca tgcagttcac gcaaggcgag ccattgcaac
      661 tggcgcttgg tcccggccgg caggaagctc gcgcgccgga ttgcagcagc gccgttgccg
      721 atggcccgca gcgccgcgcc gagtgtatcg gcatcgtcgg tagcgatcac cgatgcagca
      781 tcgtcggaaa agccgagacc ggacaaaatc gtaccggcca tcgtgagcgt cgccctgagc
      841 ggagcatcat catgccgagg gcgggcgcgc gccagaaccc gcaaggctgt cgcgccatag
      901 gcaaaggcag ccgcgatcgc ctcaaggccc agggccgtca cctcgttgac cgccagcggc
      961 aggacccggg ccggcaggcc ggcgccggca tgagcaagca gatggatcat ctcctcgcca
     1021 tgctcgtgat catggaccag cacgacggga tcctggcctc cggccgcaaa atagctggtc
     1081 agcagcgtgc gcagcgaagc gatggcgtga tccggagcgg gcagcgcata ggaggcagcc
     1141 cccgtcgggc agagggcgct gcatccgcca catccgccgc aaatgccggg gtcaatggcg
     1201 acggaatcgc cgtcaggcgt gatcgctccg gtcgggcaga tgtcaaggca gcgggtgcag
     1261 ccggtgatgc gggagcggga atgggcgcag agttccggct tgaaagcgac ataacgcggc
     1321 ttgtcgaaag tgccgacgag atcgcgcgcc tcggcgatca gccgctcgat cgccgcgcga
     1381 tccccgggcg aagcgcgcag atagccgggc cggagttcat gggcggaaaa cagcggcatc
     1441 cgtccgctga catccaggat cagatcacag gtcgaactgg ctccattgcg tggcgggcca
     1501 aagcgcatcg gtccgcgcat gccctgcacg ggcggtgcga aggaatccac cgtcagttcg
     1561 aacgcgccca gccgtccctt tgccgagcgg ataatgcccg aataaaccgg atattccgct
     1621 ccgcgcggcg gcannnnnnn nnnggggtcc ccgccagcag gattgtcagg tcgagatcgt
     1681 cggcaagccg gcgggcgacc tcaagcgccg tctcatcgct gccgtagacc acggtgatgc
     1741 cctggctttc catggaatac aacccgaagg gcggcgccgg agcgccggcc agcgccagca
     1801 atgcggccat cttcggggtc gctgccgccg cttcgtcgct gaaggcggcc ggttcattga
     1861 gattggcgaa ggtaaccggc acgtcgatcc cggcctcctc gagcgcatcc tcgatcgccg
     1921 cgctctggca ggagcaggca atcgtcagac tgctgacgga tctggccagg cgaagcagac
     1981 ggtcgcgatc gctgccgaca gggtcacgca attccacgat gtcgctgccc ggcagcgatc
     2041 gcgacaggtg ctcgacatcg cgcggctggc gaccggcgca ggagcacaga atgatggcgt
     2101 ggtgcggcag gggcattgac actcccgaaa cgtcttgatt tgaccgatcc cgctcacaca
     2161 taacgagatt aggccgccgg ctggaagcat tattgtgagc gggaggaaac aatgggccgc
     2221 aaggttgcag cagatgtgtg ggatgagcgc ttcccgccgc tgttcgagcc ggtgcgcctg
     2281 cgcgagcatg aagatgcctt cagcgtggcg atggcgcggg cggccgaaag cggcgcgggg
     2341 acggtgttcc atgtcggccg gttcgatccg atcgagtttg ccgtcgtcct ggagccggac
     2401 atgccgctgg cggcggcgcg tcgggccctc ttcgtcggga tgaatgcgct tgctgccacc
     2461 atccttgccg attgcccgcc ggaacgcgtg gtggaatttt cctatccgga cgggctggtt
     2521 ttcgacaatg gcctgattgg cggggcgagg atcgcgtttc aggatggcag gggagaccgg
     2581 gcggccgtca aggttaacga ggctggccgg aagggatcga cgagaacgcg cttcccgatt
     2641 ggctggtgtt ttcggcgatg atccgcaccg gctcgtttca ggaactggga ttcgcgcttg
     2701 acccatcagt gacctcgctg gaagaggccg gctatcgcga catcgatccg cgtggcttcg
     2761 ctgcccggtt ctgccggcat ctgatggttg aactcgacga atggcaggag gcaggtttca
     2821 aggagattgg ccgtcgctat ctcgaccggt tgccgaagcc gccgggcacc gggatgcgcg
     2881 gcatcgatcg caacggcgat ctgatcaccc atcccggcgg cggaagggct gcacagcggc
     2941 aggacttcct gccggcattg gcggcgtctg cctggttcga tccggccacc ggagcgccgc
     3001 gcgcatgaag ctgatccgta ccatcaggct tgatccttcc gacacgttcg ttttcacgcg
     3061 ctccgccgaa cctggcgaat gggcagtttc gggcgccttc gtgttctgga accagccgct
     3121 cgaagggctg gtcggcaagg aacgggcggc gtttcgctcc ggccttctgg gcattgcctc
     3181 gctgggctgg tcgacgctgg cggtggtcag cgaagcgacg gcggctgaac gcgctggcgc
     3241 ggtggaccag ctggcacgcc agcttgtcgc ccgtttcggc gcgccggatc tggcaacggc
     3301 gcgggcggcg gccgagcagg aaatcgacta tgccgagcaa ctggccgagc ctccggtcgg
     3361 gacgctgatc gccgtcaggc gcagcgacga gaacggcgac atccgcgagc gtttccgtac
     3421 gttgctgccg ggaggagacg atttgcacaa ccgcgccttt catttcgaga tgagcgacga
     3481 ggaacctgag gaacgggtcg atctgatggc tctcgacaac gcaagcaacc ctcaggggcg
     3541 cgcatgagtg acgatttctg gctggccagc ggccatcatc tcttggaccg cgacgggaac
     3601 ggcggtgggc tgctgctcac cgacgacttc ctcaaggcct atctggcgcg gccagaactg
     3661 gtgccgccgg ccgaagcctg tccggtcgag cggggcgtgc atgcagaatt gatggcctcg
     3721 ccgcggcata gggtggcgac ggcaacgctt gacgcgatcg ccgatgcgga tgcccgtgac
     3781 aatatccggg tgtttctgac cttccgcgat catctgttgc ggcacaggac gttggaggac
     3841 gcctatgtcg gcctgttccg tgcccggctg actggcattc ccgcgctgtt cgtgcagcaa
     3901 ctcgtggcag tggtggcgcg caatgccttc gatcgttgtg ccgatccctt cgtgctgcgc
     3961 gcggcagaat gccttttcag gccgcagaag gtgacgattc acgagggagc gctgctgctt
     4021 gccgatgccg aggtgatcga cagccatgag gctaaccgca agcattcgcc gctgctgacc
     4081 atgctgggcg ggccggcagt gaccgaactc agtgtgctga acgattccaa tgcggcgagc
     4141 tatttccatc gctccgatgc ccatgatctg gtgctcaacc tcttcgatcc cgcccatggc
     4201 cgcgctgccc tcggggaggc ggcacgcgtg ttcatccgtc acctttgcgg gatcgatctg
     4261 gttcttgagc cggtcgagca gctcgatggc gaaccgcagg catggttttt agccttcgat
     4321 gccgaagcga ccgccatcgg caatgccgtg tggcggggcg agatgctcga tcctgactgg
     4381 gcggcgcggg tgctggcgct ctaccgcttc cggttgcccg atgatcgccg cattgccgag
     4441 gtgcggcgtg gccgccccgg atttgcgctt gcggcaatga cgtccgaccg tatgctgaac
     4501 atcaaaccgc agaacctcat cctggggctg ccactcaacg aacgggacgg ttgaagcatg
     4561 gtggagatga ccatccgcgt cggcgtcgtc gccgaacggc gcccggtcga tcatccctgg
     4621 gtcgatcatg tctgggaagc ggtggcggtg ctgccggatg cgcccgccgt cgctccggga
     4681 acgatcctgc tcgatcaggg cggggtcgtc cggctgtatg cagggtccgc tgacatcgta
     4741 ctggcttcga gcgatacggc gcagtatcgg gacaatctga tgcggccgcc accgcggctc
     4801 tgggtggtgc tgcgggaacg acagccggag gcaagcgtcg aggttgtggc ggtcacggct
     4861 gacccgatgg aggctgaagg gcatgccgag gacagcagca atatcgtcgg tgccttgacg
     4921 atggatgccg acatggccgc cgcagtcgcg ctttttgtcg atacgcatca tgtcgagcag
     4981 gttttcgtaa agcggcagcg caagcgacat cgcgatggtg aggccgaagc atgagccgga
     5041 acgagggatt tctcagtcgc tggtcagccc ggaaactggc cccgccggaa ccagcagcaa
     5101 agccgggcga ggcagcggcg gagcagccat cgaaagagga gccggcgcaa acaggcgagc
     5161 cgttcgatcc ggcctccctg ccgtcgatcg acagtctcgt cgctgattcc gatatccggc
     5221 catttctccg ggcaggcgtg ccggaagcgc tgaaaacggc ggctttgcgc cggatgtggg
     5281 tcagcgaccc ggcgatccgc gattatatcg gccccgccga tttccagtgg gatttcaaca
     5341 cgcccggagc gttggctggc tttggcgaac tgccgcaggg aaccgatatt gttcgcatgc
     5401 tgagcgatct tggggagggg catcggccgg aatcggcgca gggtgatgat ggcgcggcca
     5461 agctgctgca agcaccggcc gatgccgggg acctgccggc cccacctgca gaatcggcga
     5521 ccgaggtcat caccggcgaa tgcccgaatt cactccctgc tcccgatgaa gaggccgcta
     5581 caaagccggt tcgccgccgc cgccacggcg ctgccacacc tgtctgagaa aaagtttccg
     5641 ttcccagcga cccggcctgg aaattattgc cccgaatgcc acgccggcgc ttgacggccg
     5701 gcccatagcc cgacactatg ggccaacttg tcagacatca tgacaaggct gcagggagac
     5761 agcgccagat catgcgggat actgggctag agcaggcgat cagggctgcg ggcgggattt
     5821 cgtcgcttgc gcgcgcgctt ggcgttgccc agccgtcagt ctcgaactgg agccgcattc
     5881 ctgccgagcg ggttctggcg gtggaggcga tcacggcagt tcccagaacg acgctgcgac
     5941 cggatctgta tcctgtcgac gcggctgcgg acgcggtcga tccggtcgat caggcgcgcg
     6001 gacaattgta tctcttgctc gccaatctgc tgctcaaagt gccgagcgat gctgtcctgg
     6061 ccgatgtcgc gcatctgccg cagggagcgc cgggtccgat cggtgaggcg ctgggtgcgt
     6121 tggccgcagc agcgcgcgag gccagggccg aggcggtcgg ccgtgagcat ttcgatcttt
     6181 tcgtcggtgt ggggcgcggc gaactggtgc cctttgcctc ctattatcgc acaggtttcc
     6241 tttatgagcg gccgctggtg aaagtgcgcg aggatctgaa gattctcggt cttcagcgca
     6301 gtgacgatct tggcgagccc gaagatggaa tcggctttct ttgcgaagtg atggccggga
     6361 tggcgctggg gcgtttcgcc gttccggaga gttacgcacg aggcttcttc aaccgccatc
     6421 tggcgccgtg ggcggagcgg ttctttgctg atctcgaagc ggccggtgcc gcgcggttct
     6481 atcgcgcggt cggtcggctc gggcggagtt ttattgggct cgaacgcgag gcgttcgcgc
     6541 tggatgatga tagccatgtg gcggcatcga tcgcgcatgg gaccaattcg gaggggaaac
     6601 atgaccacaa agcgtgctga cgaaacgctg aaccgccgcg gattcctgcg cgccggtctc
     6661 ggcacggctt ccgtcgcggc cacaacggtg atcggtgcgg ctcagcccgc gcaggcggct
     6721 gagaacgacg ccgagcggaa aaaggcccgc tacaaggaaa cggaccacgt caagacgttc
     6781 taccgcacca atcgctactg aggacgctcc catgctgatc aagcgcaagg caggacaggt
     6841 tcggcgcggc gcctcggcgg gcgcctttgc cgccatcaac ggcgcgggga tggaccgccg
     6901 cgcctttctt cgcaactccg gactgacggc aggcggcctg gccgcgttgt cgagcctgac
     6961 cttcgcaggc gtgcagcgcg ccgaggctgg tccggtggcg gcggatgtcg ccatcacccg
     7021 caagcgcaac atgtgcacgc attgctcggt cggctgttcg gtgattgccg aggttcagaa
     7081 cggggtctgg gttggtcagg agccggcctg ggacagcccg atcaatcgcg gaacccattg
     7141 tgccaagggc gccgcatcgc gcgaactggt caagggagag cggcgcgtca agaccccgat
     7201 gaagctggtc aacggccagt ggcagcggat ctcgtgggat caggcgatcg acgagatcgg
     7261 cagcaagatg ctggagatcc gcgagaaatc cgggccggat tcggtctatc tgctcggctc
     7321 ggccaagttc accaacgagg cggcctacct gttccgcaag ttcgcggcgt tctggggcac
     7381 caactcggtc gatcatcagg cgcgcatctg tcattcgacc acggtggcgg gcgtcgccaa
     7441 tacctggggc tatggcgcac agaccaattc ctacaacgac atccgcaacg ccaagaccgt
     7501 gatcttcatg ggttcgaatg cggccgaagc gcatccggtg tcgctgcagc acatcctgac
     7561 cggcaaggag acgcagcgcg ccaacgtgat cgtgttcgat ccgcgcttca cccgcacggc
     7621 ggcgcattcg accgagtttg tgcgcttccg ctccggcacc gacattccgg tgatctgggg
     7681 catgctgcac cacatcttca agaacggctg ggaggacaag ggattcatcc agagccgcgt
     7741 ctacggcatg gaaatggtgc gcaaggaagt cgagaagtgg acgcccgagg aggtcgagaa
     7801 ggtcaccggc attcccggcg agcagctcaa gcgcgtcgcc gagaccttcg ccaagcagcg
     7861 cccgtcgacc ttcatctggt gcatgggcgg cacgcagcat acggtcggca ccgccaacgt
     7921 ccgcgcctat tgcaccttgc tgctggcgac cggcaatgtc ggccagttcg gcaccggcgc
     7981 caatatcttc cgcggccaca ccaacgtgca gggcgcgacc gacctcggtc tcgatatcgg
     8041 caacctgccg ctctattacg gccttgccga gggcgcctgg cgccattggg cccgcgtctg
     8101 ggatgtgccg tatgattatc tgctgtcgcg cttcgtcgac aagaagatga tggaggcacc
     8161 cggcatcccg tcgacccgct ggttcgacgc cgtgacgctg ccgaaggatc aggtggacca
     8221 gaaggacaac gtgaaagcga tgttcatcat gggccatggc ggcaacacca tcccgcgcat
     8281 gccggattcg gccaagggcc tggaggcgct ggaactcttg gtggtggtcg atccgcatcc
     8341 gacaacgttc tcggtgctgt ccaaccgcaa gaacggcagc tacatcctgc cggcctgcac
     8401 ctcgttcgag atggacggct cgcggacggc ttcgaaccgc tcgatccagt ggggcgagaa
     8461 ggtggtcgag ccgatcttcg aatcgaagaa cgattacgag atcatctacc gcttcgccag
     8521 gaagctcggg tttgccaccg agatgttcaa gaacatcacc gtcaacggcg tccagccggt
     8581 gccggaggat atcctgcgcg agatcaacaa gggcggcctt tcgaccggct attccggaca
     8641 gtcgccggag cgcctcaagc tgcatatggc caaccagggc gccttcgatg tggtgtcgct
     8701 caaggcggcc gatggtccgg ccaagggcga ttactacggc ctgccgtggc cgtgctgggg
     8761 caagcccgag cacaagcatc ccggcacgca cacgctctac aacaccaacc tgcatgtgaa
     8821 ggacggcggc ggcacgttcc gcgcccgctt cggcgtcacc tatgaggaac cacaggcgga
     8881 cggatcgaaa aagacgatca acatgctgtc ggaaggctcc tattcggtcg gctccgagat
     8941 caaggacggc tatccggagt tcacctacgg cgtgctcaag aagctcggct gggatatcga
     9001 cctcaggccg gacgagattg agacgatcct gaagatcggc ggcaacaacc ccgatggcgt
     9061 cggctgggcg gtggacctca gctgcggcat catccgtgtc gcgctcgaac acggctgcat
     9121 tccctacggc aacggcaagg cccgcgccaa tgcgtggaac ctgccggacc cgatcccggt
     9181 gcatcgcgag ccgatctaca caccgcgccc ggaactggtg gcccagtatc cgacacggcc
     9241 ggacggcaag cagttccgcg tgccgaacat cggcctctcc atccagaagg atgcggtcga
     9301 gaaggggatt gccaagcagt tcccgatcat cctcacctcc ggccgtctgg tcgaatacga
     9361 gggcggcggt gaggagaccc ggtcgaatcc gtggctggcc gagctgcagc aggacatgtt
     9421 cgtcgagatc aacccgaacg acgcgcgctc ccgtggcatc tccgatggcc agtgggtgtg
     9481 ggtgctcggc ccggtgggcg gggccaaggc caaggtcaag gcgctggtta ccgagcgtgt
     9541 cgggcgcggg gtggcgttca tgccgttcca cttcgccggc tggtatcagg gcgaggatca
     9601 gcgaccgaag tatccaaagg gtgccgaccc ggtggtgctc ggcgagagcg tgaacatcgt
     9661 caccacctac gggtatgatc cggtcaccgg catgcacgaa ggcaaggcca cgctttgtga
     9721 catcaaggcg gtctgaggag aacacacatg gcccggatga aattcctctg cgactccgat
     9781 cgctgcatcg aatgcaacgc ctgcgtcacc gcctgcaaga acgagcacga ggtgccgtgg
     9841 ggcatcaacc gccggcgcgt ggtgacgctc aatgacggcc tgccgggtga acgctcggtg
     9901 tcgatggcct gcatgcactg cacggatgcg ccctgcgccg ccgtctgccc ggtgaactgc
     9961 ttctacacca ccgccgatgg cgtggtgctg cattccaagg acctgtgtat cggctgcggc
    10021 tactgcttct acgcctgccc gttcggcgcc ccgcaatatc cgaaggtcgg caacttcggc
    10081 tcgcgcggca agatggacaa gtgcacctat tgtgccggcg ggccggaaga cgaccggacc
    10141 aaggcggaat atgaaaagta tggccgcaac cgtctcgccg agggcaaatt gccgctgtgc
    10201 gccgagatgt gctcgaccaa ggcgctgctg gccggtgatg gcgacatcat cgcccagatc
    10261 tacaaggagc gggtcgccaa gcgcggctat ggctccggcg cctggggctg gcaggtcgcc
    10321 tacaaggaaa ccgtcgcgct gtgatttatc gattgggcac cgggtccggc ccggtcgccc
    10381 tcatgcattc cgggaggcac acttggtctc gaacatgaca catcctttcc gttggctggc
    10441 gctgctggca gcgctggtct ttccgctgct tgtctcggcc gtgccggctt cggcccaaca
    10501 gggccagcag atcaatccga cggcgcaatc gctgaaggaa gcggagttgc tcaaggcgct
    10561 gaagggcggc gatgcgattt ccggccgcat ttccatcccc gatgccaagg cggcgacgct
    10621 gatccagccg gacgggcgga gctggcagga tttcaagcgg tcgacgctgc cgatgctcgg
    10681 cgcgctgctg atcctcggca tgctggcgct gctggtgctg ttctacgcta tccgcggtcg
    10741 catccgcatt gattcgggcc cgtcgggcca gttcatcgag cggttcggag ccattgaccg
    10801 gttcgttcac tggctgacgg cgtcgagctt catcatcctg ggccttaccg gtctcaacgt
    10861 gaccttcggg cgctccatcg tgttgccgat cgtcgggccg gaagcgttca cgacactgag
    10921 cgaattcggc aagatgctgc acaattacgt cggctttgcc ttcatggtcg gcgtggtgct
    10981 gaccttcctg atctgggtca aggataacct gccgcattca cgcgatctgg tctggctgag
    11041 ccatttcggc gggctgttct cgaaggggga ccacccgccg gcggagcgct tcaatgcggg
    11101 gcagaagggc atcttctggt cggtggtcat cggcggcgcg ctgatgagcc tgtccggcct
    11161 gcatctgatc ttccccaatt cagtgggcgg cggggtggcc gagatccagt tccattcgat
    11221 gatccacggt atcgtggcga tggtgatggt ggcgatcatc cttgcccata tctacatcgg
    11281 ctcggtcggc atggaaggcg ccaaggaagc gatgacgacc ggcgaggtcg atctgaactg
    11341 ggccaaggag catcattcgc tgtgggtcga gcgcgaattg aaggccaagg ggcaggccat
    11401 cgtgcatccg gccgctgccg aatagtcgca tcgaccgaac aggcttgcaa aagggcgcga
    11461 cttgcgccct tttcgcattt catggcaggg accaggcagt gaagatcatc ggcatcaccg
    11521 gctggagcgg ggcgggcaag accagcctga tcgtcaagct cattccggaa ctcagggcgc
    11581 gcggattgac cgtctcgacc ctcaagcacg cccatcatgg ctttgatgtc gatgtgccgg
    11641 gcaaggattc gcatatgcac cggacggcag gcgctaccga agtcctggtc tcgtccgggc
    11701 gccggttcgc gctgatgcat gaactcaacg gcgaggcgga gtggtcgttg ccgcgcctgc
    11761 tcggcaagct caatccggtc gatctcgtgc tggtggaagg gttcaagcgc tatccccacc
    11821 ccaagatcga ggtctacagg gtggcgaacg gcaagccgat gctgcacccc ggagacgcca
    11881 gcatccgcgc ggtcgcggcc gattgtccgc tgccggaggc tgggcgaacg gtctatcata
    11941 tcgatgacct gacggggctg gccgaggcgg tgctatccct gtccgaaccg gccggatcgg
    12001 tgtttgcgga gcttgggtga tggcgcaact cagtgacgat tgttttgctt tcggcggccc
    12061 gctgatgacc gtcgacgagg cgatcgcgct gatcggggaa cggatccagc ccgtggccgg
    12121 cgaggaccgc gtgggctcgg ccgaagcctg cgggcgcatc cttgccgaac ccgtgactgc
    12181 gagtgtggcg gtgccgtcct tcgacaattc ggcggtggat ggctatgccg tccgtcatgc
    12241 cgatcttgcg gcggagggcg tgacgacgct gccggtcagc gcgcgcattg ccgcaggctg
    12301 tcgtaccctg gctccgctta cgccgggaac ggcagcgcgg atcttcaccg gcgctccgat
    12361 gccggagggc gccgacacgg tgttcatgca ggaagatgtg caggaagatg gcgggcgagt
    12421 gacgctgcct cctggcctca agcagggcgc caaccgccgc ttcgccggcg aggacttcaa
    12481 actcggggcc gaggtgctgg ccgccgggac gcggctcgat ccgcggcaca
//
LOCUS       DAOMXG010000060        12330 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_2786_length_12330_cov_9.381181, whole genome shotgun sequence.
ACCESSION   DAOMXG010000060 DAOMXG010000000
VERSION     DAOMXG010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 12330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12330
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2786_length_12330_cov_9.381181"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="PLQ11_05810"
     CDS             <1..832
                     /locus_tag="PLQ11_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009762642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRK24452.1"
                     /translation="GSPWRRLVGQPPVLWAAGFLLAVLACGLLAPWIMPLDPAAANYA
                     RLLEGPSRDHWLGTDEFGRDLATRLIHGAPLSLGIGLVAAAGGAVLGTAFGIASGMAG
                     GWLDRLAMRFCDVLLAFPGLLLALAIVALLGPGLANVGWAVAIASVPVFARLARAATL
                     TQLEAGHVEAARLLGVLPVDIAVRHVLPGAVGGVLAYFWLRAGTAILTAAGLSFLGLG
                     AQPPAPEWGAMLAAGRSYIGVADHLMLVPGLAIFLSVLALTIIGDGVRDALDPRLPPA
                     "
     gene            914..2746
                     /locus_tag="PLQ11_05815"
     CDS             914..2746
                     /locus_tag="PLQ11_05815"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HRK24453.1"
                     /translation="MNGPTLPTFQPRRSGASTASEALPEWQLDDLYPGMDSAAYRDDL
                     ARLEGLCRDFANQYRGKVADVAAGPDGAAALFAAIQAYEAIEDRLGRVISYAGLVYAG
                     DTTDPKRGKLYGDAQEKVTAASSDLIFFQLELNRLDDALFARLIRTAPLDHYRPWLED
                     IAKEKPYQLDDRLEQLFHEKSVTGRAAWNRLFDETIASLRFKIDGKELTLEPTLNFMQ
                     DPDEKKRKAAAGALSATFKENLRTFTLITNTLAKDKEIGDRWRGFADVAESRHLANRV
                     EPEVVDALVEAVQAAYPRLSHRYYALKARWFGKDKLEFWDRNAPLPKVEQRAISWGEA
                     RDTVLDAYSSFAPEMARIARRFFDEGWIDAPVRPGKAPGAFAHPTVPSSHPYVLVNYQ
                     GKPRDVMTLAHELGHGVHQVLAAPNGALMAPTPLTLAETASVFGEMLTFRKVLAATAD
                     QRQKKALLAGKVEDMINTVVRQIAFYSFERKVHLARKEGELTAEAINDIWMSVQGDSL
                     GPSIRLSDGYEVYWAYIPHFIHSPFYVYAYAFGDCLVNSLYGVYQKADPRFVENYFAL
                     LSAGGTKHHSELLAPFGLDARDPRFWQIGLTMIEGMIGELEGMP"
     gene            complement(2753..3412)
                     /locus_tag="PLQ11_05820"
     CDS             complement(2753..3412)
                     /locus_tag="PLQ11_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020180516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HRK24454.1"
                     /translation="MPIRLAILATLGLALLAAWPAVAEPPPSAGRAACVHAAPTCTSW
                     QARLVAAASAQVGVTRLYDPAYARIGFPNGDVPRERGVCTDVIIRAYRDAFGLDLQTL
                     VNADMKRAFSAYPRRWGLARPDTNIDHRRVGNLQVYFSRHGQRLPPSRNPDDFKPGDI
                     ISQMLPGNLPHIAVVSARRGASGAPEVIHNIGAGVRSEDTLFAYPITGHYRFAPAADR
                     R"
     gene            3605..4027
                     /locus_tag="PLQ11_05825"
     CDS             3605..4027
                     /locus_tag="PLQ11_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24455.1"
                     /translation="MTDAKKHIFISYSRQGGMALARRVSAALSGMGFDADVPIQPIWE
                     PDAEWDDEVVADIRTAAGVVFIEPKDERAAPHLMFEIGAAHALGKKIVGVGANREEFY
                     LEEAGSDGVLHIYDLIDATNLSDEELAVAIVAGLGLTS"
     gene            4104..5459
                     /locus_tag="PLQ11_05830"
     CDS             4104..5459
                     /locus_tag="PLQ11_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HRK24456.1"
                     /translation="MSASDDDEKNRLSARATRYARVGMHVGGAAARVAGTRLFGGSGT
                     GDAAALAGALGRLKGPLVKVAQLMATIPDLLPADYAAELQKLQSQAPPMGWVFVKRRM
                     ASELGPDWQARFQSFEKDAAAAASLGQVHRAVSLDGQALACKLQYPDMASVVEADLAQ
                     LRLLFAVHRRMDGVIDTSQAAEEIAERVREELDYVREAGHARLYKRVLADEPLVRVPE
                     VFDGLSTRRLITLGWLDGSKLLDHRQDDLESRNRLATAMFKAWWLPFSRYGVIHGDPH
                     LGNYTVFDEGGRAGGINLLDYGCIRIFPPGFVGGVVDLYYGLRTGDRNRVVHAYETWG
                     FKALNPALIDTLNIWARFIYGPLLEDRVRSIADGVSPAEYGRREAMRVRSELKKHGAL
                     TVPREFVFMDRAAIGLGGVFLHLRAELNFYRLFNAAIEGFDSADVAQRQGSAMQGAGL
                     A"
     gene            5570..5776
                     /locus_tag="PLQ11_05835"
     CDS             5570..5776
                     /locus_tag="PLQ11_05835"
                     /inference="COORDINATES: protein motif:HMM:NF019452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aa3-type cytochrome c oxidase subunit IV"
                     /protein_id="HRK24457.1"
                     /translation="MAEHHGSHRVDNDYVEHEKTYNLFVTLTKWGTVASLLIVLFAGS
                     MTSLVPWALTLVLAVLMFVAAMML"
     gene            5830..6969
                     /locus_tag="PLQ11_05840"
     CDS             5830..6969
                     /locus_tag="PLQ11_05840"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HRK24458.1"
                     /translation="MRIVVLDEAKSGEPRVAVTPETVKKLIGFGAEVTVQAGAGASSS
                     FPDAEFEGAGAKVTKDMKAALAEADILLSVGRPDKGRIKGIRKGALALATMDPYGNAE
                     AIQGLADTGVAAFAMELMPRITRAQVMDVLSSQANLAGYRAVIEAAGEYGRAFPMMMT
                     AAGTVPAAKAFIMGVGVAGLQAIATARRLGAVVTATDVRPATKEQVESLGAKFLAVED
                     EEFKQAQTAGGYAKEMSKEYQAKQAELTASHIAKQDVVITTALIPGRPAPRLITKEMV
                     ASMRPGSVVVDLAVERGGNCELARKGEVVTSDNGVKIVGWGNLAMRLPQTASGLYSKN
                     LVAFLETMIDKKEKKLSVNWDDELVKATLLSKDGALVHPMFQPKS"
     gene            6981..7427
                     /locus_tag="PLQ11_05845"
     CDS             6981..7427
                     /locus_tag="PLQ11_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012319752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-translocating transhydrogenase family
                     protein"
                     /protein_id="HRK24459.1"
                     /translation="MANSPAELVEQARQAAAAAKAASDKAQAALDMLQGAADAAGAAA
                     SAATGGAVDPFIFRFAVFVMAIFVGYYVVWSVTPALHTPLMSVTNAISSVIIVGALLA
                     TGVQISESGSGWAVVFGVIALIFACVNIFGGFLVTQRMLAMYKKKG"
     gene            7432..8835
                     /locus_tag="PLQ11_05850"
     CDS             7432..8835
                     /locus_tag="PLQ11_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HRK24460.1"
                     /translation="MNANFANLLYLVAGVLFILSLRGLSHPTTSQQGNKFGMIGMGIA
                     VVTTLLLSPPASFMNWLFLLVGAGVGGGIGALLAQRIAMTAMPQLVAAFHSLVGLAAV
                     FVAAAALYSPAAFGIGAPGAIKGASLFEMALGAAIGAVTFTGSVIAFLKLDGRMSGAP
                     IMLPQRHAINIGLGVLLAFFILVFIRSESTAVFWLIVLVSFALGVLLIIPIGGADMPV
                     VVSMLNSYSGWAAAGIGFTLGNLALIITGALVGSSGAILSYIMCKAMNRSFFSVILGG
                     FGGEAVAGGAAQEARPYKAGSADDAAYIMKNASKVIIVPGYGMAVAQAQHALREMADL
                     LKKEGVQVSYAIHPVAGRMPGHMNVLLAEANVPYDEVFELEDINSEFAQADVAFVIGA
                     NDVTNPAAKTDKTSPIYGMPVLDVEKAANVLFVKRSMGGIGYAGIDNDLFYRNNTMML
                     LADAKKMVENIVKALAH"
     gene            complement(8945..9859)
                     /locus_tag="PLQ11_05855"
     CDS             complement(8945..9859)
                     /locus_tag="PLQ11_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRK24461.1"
                     /translation="MLAAAMKSSCSHAALRQSRGVRTWARVPVLVPVLALVLAMGACE
                     TISPINPAGVAEVDDASAAAASSANIGSLSDVIARNPDDPQAYNTRGAAYARAGRFND
                     AINDFSRAVRINPNFAAAYVNRGLAHRQIGKNDLAFGDFNRAIQADPNYPAAYLARGN
                     LYRAQGNMQSANEDLSQALRLKPDMAEAYHGRGLIHQLQGQHPLAISDFDAAIDRNPY
                     VAPPYLGRGQSLNAIGKFEQALEDFTATLNIDNRQADGWAGRGYALDRLGRKAEANEA
                     YQRALNLNPNVVLPKDASVSRGGFGLFR"
     gene            complement(10021..10257)
                     /gene="rpsU"
                     /locus_tag="PLQ11_05860"
     CDS             complement(10021..10257)
                     /gene="rpsU"
                     /locus_tag="PLQ11_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HRK24462.1"
                     /translation="MQVLVRDNNVDQALRALKKKMQREGIFREMKLRGHYEKPSEKRA
                     REKAEAVRRARKLTRKRLQREGLLPAAKPKVKKV"
     gene            complement(10401..11489)
                     /locus_tag="PLQ11_05865"
     CDS             complement(10401..11489)
                     /locus_tag="PLQ11_05865"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HRK24463.1"
                     /translation="MNRLVPRQTIGILGGGQLGRMAALAAAELGLRTLIYQPEREGPA
                     FDVATECMTGDYEDAEALARFAARCDVITYEFENVPARTVEILAPLKPVRPGARMLSI
                     SQDRLHEKTFMSDLGIETAPFWSVDSLTELEVAVLALGRPSVLKTRRFGYDGKGQVVI
                     RPDTALPAAYDAIGGAPAILEGFVPFSAEVSVIAARGLDGTFAAYDVCENEHRNHILW
                     QTNVPARISPETQARALDIARRIGDGLDAVGVFAVEMFLLRDGGSERLIVNEIAPRVH
                     NSGHWTIEGAETSQFEQHIRAIAGWPLGSVACTGASVTMQNLIGDEAGDWARLLAEPG
                     AHLHLYGKSEPRPGRKMGHVTRVRRHAG"
     gene            complement(11486..11983)
                     /gene="purE"
                     /locus_tag="PLQ11_05870"
     CDS             complement(11486..11983)
                     /gene="purE"
                     /locus_tag="PLQ11_05870"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019998225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HRK24464.1"
                     /translation="MTGTPARVAIIMGSQSDWSTMRHAADTLEALGIAFDARIVSAHR
                     TPERLFSFARSAREAGYHVIIAGAGGAAHLPGMTAAMTPLPVFGVPVESKALSGVDSL
                     HSIVQMPAGIPVGTLAIGRAGAVNAALLAAAVLALHDPALATRLDGWRSAQTAAVAER
                     PRDEP"
BASE COUNT         2137 a         3729 c         4152 g         2312 t
ORIGIN      
        1 cggctcgcca tggcggcggc tggtggggca gccgccagtg ctgtgggcgg ccgggttcct
       61 gctggcggtt ctagcttgcg ggctgctggc tccgtggatc atgccactgg acccggctgc
      121 ggccaattat gcacgcctgc tggaggggcc aagccgggat cactggctcg gcaccgatga
      181 atttggccgc gatcttgcca cccggctcat tcatggagcg ccgctgtcgt tggggatcgg
      241 gctggtcgcc gctgcgggcg gggcagtcct tgggacggca ttcgggatcg cctccggcat
      301 ggcgggcggc tggctcgacc ggctggcgat gcggttctgc gatgtgctgc tggcctttcc
      361 cgggctgctg ctggcgctgg cgatcgtcgc gctgctcggg cctgggctgg ccaatgtcgg
      421 ctgggcggtc gcaatcgcgt ccgtgccggt ttttgcgcga ctggcgcgcg ctgcgacgct
      481 gacccagttg gaggccggcc atgtggaagc ggcccgcctg ctcggcgtcc tgcccgtcga
      541 cattgccgtt cgtcatgtcc tgcccggcgc ggttggcggt gttctggcct atttctggtt
      601 acgcgccggc actgcgatcc tgacagcggc cggattgtcg tttctggggc tcggggccca
      661 gccgccggca ccggaatggg gcgcgatgct ggcagcgggg cgcagctata tcggggttgc
      721 cgaccatctg atgctggttc cggggctcgc catctttctc agcgtgctgg cgctgacgat
      781 catcggggac ggcgtgcgcg atgcgctcga tccgcgtctg ccgcccgcat gacgacatca
      841 ccgcatcgtt ggttgaaaca accggcgatt gcccgatatt gagcgtgaca gattcgctcc
      901 ggagaccgca cgcatgaacg gccccaccct gccaaccttc cagccgcggc gcagcggcgc
      961 cagcacggcg agcgaggcgc tgccagaatg gcagctcgac gatctctacc cgggaatgga
     1021 ttcggcagcc taccgcgatg atctggcccg gctggagggt ttgtgccggg attttgccaa
     1081 tcagtatcgt ggcaaggttg cggacgttgc tgccggaccg gacggcgcgg ccgcgctgtt
     1141 cgcggcgatc caggcctatg aggcgatcga ggacaggctt ggccgggtga tttcctatgc
     1201 cggtctcgtc tatgcgggag acaccaccga tcccaagcgc ggcaagctct atggcgatgc
     1261 ccaggagaag gtgacggcgg caagttccga cctgatcttc ttccagcttg aactcaaccg
     1321 gctggatgat gccctgttcg cccggctgat caggacggcg ccgctcgatc actatcggcc
     1381 ctggcttgag gatatcgcca aggaaaagcc ctatcagctc gacgaccggc tggagcagtt
     1441 gttccacgaa aaatcggtca ccggtcgcgc ggcctggaac cgcctgttcg acgagaccat
     1501 cgcatccctg cgtttcaaga tcgacggcaa ggagctgacg ctcgagccga cgctcaattt
     1561 catgcaggat cccgacgaga agaagcgcaa ggccgctgcg ggcgccttgt cggcgacctt
     1621 caaggaaaac ctgagaacct tcacgctgat caccaacacc ctggccaagg acaaggagat
     1681 cggtgatcgc tggcgcggct ttgccgatgt ggcggagtcg cgccatctcg ccaaccgggt
     1741 cgagccggag gtggtcgatg ctctggtcga ggcggtgcag gcggcctatc cgcggctgtc
     1801 gcatcgctat tacgcgctca aggcaaggtg gttcggcaag gacaagctgg agttctggga
     1861 tcgcaatgca ccgttgccga aggtggagca gcgggcgatt tcctggggcg aagcgcgcga
     1921 cacggtactc gatgcctaca gcagctttgc cccggaaatg gccaggatcg cccggcgctt
     1981 cttcgatgag ggctggatcg acgcgccggt gcggccgggc aaggcgccgg gcgcctttgc
     2041 ccatcccacc gtgccgtcat cgcaccccta tgtactggtc aactatcagg gcaagccgcg
     2101 tgatgtgatg acgctggccc atgagttggg ccacggtgtg catcaggtgc tggcggcgcc
     2161 caacggcgcg ctgatggcgc cgaccccgct gacgctggcc gaaaccgcca gcgtgttcgg
     2221 cgagatgctg accttccgca aggtgctggc cgcgaccgcc gaccagcggc agaagaaggc
     2281 actgctggcc ggcaaggtgg aggatatgat caacaccgtg gtgcggcaga tcgcgttcta
     2341 ttcgttcgag cgcaaggtgc atctggcccg caaggaaggc gaactgaccg ccgaggcgat
     2401 caacgacatc tggatgagcg tgcagggcga cagcctcggt ccgtccatcc ggctgtcaga
     2461 cgggtatgag gtctactggg cctatatccc gcatttcatc cattcgccgt tctatgtcta
     2521 tgcctacgct ttcggcgact gcctggtgaa ttcgctctat ggcgtctacc agaaggccga
     2581 tccaaggttt gtcgagaact acttcgcgct gctctcggca gggggcacca aacatcattc
     2641 cgagttgctc gctccgttcg ggctcgatgc gcgtgatccc aggttctggc agatcggcct
     2701 gacgatgatc gaaggcatga tcggcgaatt ggagggaatg ccctgaggcg gcctatcgcc
     2761 ggtccgctgc tggcgcaaac cggtagtggc cggtgatcgg ataggcaaac agcgtgtcct
     2821 cgctgcggac tcctgcgccg atgttgtgga tgacttccgg cgccccggac gcgccacggc
     2881 gtgcgctgac tacggcgata tggggcagat tgccgggcag catctggctg atgatgtcgc
     2941 cgggcttgaa atcgtccgga ttgcggctgg gcggcaggcg ctggccgtgc cggctgaaat
     3001 agacctgcag attgccgacc ctccgatggt cgatgttggt gtcagggcgc gcaaggcccc
     3061 agcggcgcgg ataggctgaa aatgcgcgtt tcatgtcggc attgaccagc gtctgcaggt
     3121 cgaggccgaa ggcatcgcgg taggcgcgga tgatcacatc cgtgcacacg cctcgctcgc
     3181 ggggcacatc gccgttggga aagccgatgc gggcataggc cggatcgtag agccgggtca
     3241 ccccgacctg tgcggaggcg gcggcgacga gccgggcctg ccaactggtg caggttgggg
     3301 ccgcatgcac acaggcggcc cgcccggcag aaggcggcgg ttcggccacg gctggccagg
     3361 ccgcaagcag cgctaggcca agtgtggcaa ggatggcaag tcggattggc atggaaggtc
     3421 tcggctcagg tcggcttgcc atttccgaca ggattttcgg gcaaaagcat ggccactcag
     3481 ccggcgatgg tgcctgcctg acagggcatc agcgcccgcg ggccagttga ggcaaatcgt
     3541 ggcatggatt ttcctgcgcc aagccgctag acagccgccg gattaccgac aacggacccc
     3601 ggtcatgacg gacgcaaaga agcacatctt catttcctat tcgcgccagg gcggcatggc
     3661 gctagccaga cgggtcagcg ccgcgctgtc cggcatgggc tttgatgccg atgtgccgat
     3721 ccagccgatc tgggagccgg atgcggagtg ggatgacgag gtggttgccg atatccgcac
     3781 cgccgccggg gtggtgttca tcgaaccgaa ggatgagcgg gccgcgccgc atctgatgtt
     3841 cgagatcggc gcggcccatg cgctgggcaa gaagatcgtc ggcgtcggcg ccaaccgcga
     3901 ggaattctac cttgaggagg cgggcagcga cggcgttctg catatctatg atctcatcga
     3961 tgcgaccaat ctgtccgatg aggagctggc cgttgccatc gttgccgggc tcggcctgac
     4021 gtcctgagac ctgcccgact gcgccggttt ccctgtccgg acccgggctg gcgggaaagc
     4081 tcgcgcatgc ctatttatgc ctgatgagcg cttcggatga tgacgaaaag aaccggcttt
     4141 cggcgcgagc cacccggtat gcgcgggtcg gcatgcatgt cggcggtgcg gcggcgcggg
     4201 tggcaggcac gcggctgttc ggtggatcgg gaacgggcga tgccgcagcc ctcgccggag
     4261 cgctcggccg gttgaaggga ccgctggtca aggtggcgca attgatggcc acgatcccgg
     4321 atctgcttcc cgctgattac gcagccgaac tgcagaagct gcaatcccag gccccgccga
     4381 tgggctgggt gttcgtcaag cggcgcatgg ccagcgaact cgggcccgac tggcaggcgc
     4441 ggttccagag tttcgaaaag gacgcggctg cggcagcttc gctggggcag gtgcaccggg
     4501 cggtttcgct cgacgggcag gcgctggcct gcaagctgca atacccggac atggcctcgg
     4561 tggtggaggc ggatctggcc cagcttcgcc tgttgtttgc cgtgcatcgg cgcatggatg
     4621 gcgtgatcga tacctcgcag gccgccgagg agatcgccga gcgggtgcgc gaggagctcg
     4681 actatgtgcg tgaggccggc catgcgcggc tttacaagcg ggtgctggcg gacgagccac
     4741 tggtgcgcgt gcccgaggtg ttcgacgggt tgtcaacacg gcggttgatc acgctcggct
     4801 ggctcgatgg cagcaaactg ctcgaccacc ggcaggacga tcttgaaagc cgcaaccggc
     4861 tggcgacggc gatgttcaag gcctggtggc tgccgttttc ccgctacggc gtcattcatg
     4921 gcgatccgca tctgggcaat tacacggtct ttgacgaggg cggccgggca ggggggatca
     4981 atctgctcga ttatggctgc atccgcatct tcccgcccgg gttcgtcggc ggtgtggtcg
     5041 atctctacta cgggctgcgc acgggtgacc gcaaccgggt ggtgcatgcc tatgagacct
     5101 ggggattcaa ggctctcaac ccggcgctga tcgatacgct caacatctgg gcgcggttca
     5161 tctacgggcc gttgctggaa gatcgggtgc ggtcgattgc cgatggggtc agtccggccg
     5221 agtatggccg ccgcgaggcg atgcgggtgc gctcggagct gaaaaaacac ggcgcgctga
     5281 ccgtgccgcg cgagttcgtg ttcatggatc gggcggccat cgggctcggc ggagtgttcc
     5341 tgcacctgag ggcggagctg aacttctacc gcctgttcaa tgcggcgatc gagggctttg
     5401 acagtgccga tgtcgctcag cgccagggca gtgcgatgca gggcgcgggc cttgcctgat
     5461 ttcggtcaag tggacggcgg aaaaattgcc acacccgacc attgcggcat tgcgaggcgc
     5521 aggctagtgc gtaatgtgtt gtcagaattt ggcgcctaga ggggtttcca tggccgagca
     5581 tcacggatcg caccgtgtcg acaatgatta tgtcgaacac gaaaagacct acaacctgtt
     5641 tgtcaccctt acaaaatggg ggacggtggc aagtctgctc attgtcctgt tcgctggctc
     5701 gatgacaagt cttgtgccgt gggcgctgac tctggtgctg gcggtgctga tgtttgtcgc
     5761 agccatgatg ctctgaaccc gtggcggggc cggcggcccc gttcgcgttt ccatctgcga
     5821 ggagtgacga tgcgcatagt cgttcttgat gaggccaagt ctggggagcc gcgcgttgca
     5881 gtcaccccgg agaccgtgaa gaagctgatc ggcttcggag ccgaggtgac ggtgcaggcc
     5941 ggcgccggcg cgtcgtcgag tttccctgac gccgagttcg agggggctgg agccaaagtc
     6001 accaaggata tgaaggccgc actggccgag gcggacatcc tgttgtcggt cggcaggccg
     6061 gacaaggggc ggatcaaggg catcaggaag ggcgcgctgg ctctggccac catggatcct
     6121 tacggcaatg ccgaggcgat ccagggattg gctgataccg gtgttgccgc ctttgccatg
     6181 gagttgatgc cgcgcatcac ccgggcccag gtcatggacg tgctgtcctc gcaggccaat
     6241 cttgccggct atcgcgccgt gatcgaggcc gccggggaat atggccgggc ctttccgatg
     6301 atgatgaccg cagccggcac ggttccggcg gccaaggcgt tcatcatggg cgtcggcgtc
     6361 gccggcttgc aggcgattgc caccgcgcgc cggctcggcg cagtagtgac ggcgaccgac
     6421 gtgcgccccg ccaccaagga gcaggttgaa tcgctcggcg ccaagttcct cgcggttgag
     6481 gatgaggagt tcaagcaggc gcagaccgct ggcggctacg ccaaggaaat gagcaaggaa
     6541 taccaggcca agcaggcgga attgacggcc agccacattg ccaagcagga tgtcgtcatc
     6601 accaccgccc tgattccggg ccggccagcg ccgcgcctga tcaccaagga aatggtggca
     6661 tcgatgcgtc cgggctcggt cgtcgtcgat ctggcggtgg agcgcggcgg caattgcgag
     6721 cttgcccgca agggtgaggt cgtcacatcc gacaatggcg tgaaaatcgt cggctggggc
     6781 aatctggcga tgcgcctgcc gcagaccgcc tcgggcctct attccaagaa ccttgtcgcg
     6841 ttcctcgaga cgatgatcga caagaaagag aaaaagctca gcgtcaactg ggatgacgaa
     6901 ctggtgaaag ccacgctgct gtcgaaagac ggtgcgctcg tccacccgat gttccagccc
     6961 aagtcctaag ggggctatcc atggccaatt ctccagccga actggttgaa caggcgcgtc
     7021 aggcggccgc tgccgccaag gctgcgtccg acaaggcgca agccgcgctc gatatgttgc
     7081 agggggctgc cgatgcagcc ggtgcggcgg cctcggccgc cacgggcggc gcggttgatc
     7141 cgttcatctt ccgctttgcc gtgttcgtga tggcgatttt cgtcggctat tatgtggtct
     7201 ggtcggtgac accagcgctg catacgccgc tgatgagcgt taccaacgct atttcctccg
     7261 tgatcattgt cggcgccttg ctggcgacgg gcgtccagat cagcgaaagc ggttccggtt
     7321 gggccgtcgt tttcggggtg atcgcgctga tcttcgcctg cgtgaacatc ttcggcgggt
     7381 tcctggtcac ccagcgcatg ctggcgatgt acaagaagaa gggctgagcc gatgaacgcc
     7441 aatttcgcca atcttctcta tctcgtcgcg ggcgtgctgt tcatcctgtc gctgcgtggc
     7501 ctgtcgcatc ccacgacctc gcagcaggga aacaagttcg gcatgatcgg catgggcatt
     7561 gccgtcgtga ccacgctgct gctgtcgccg ccggcgtcgt tcatgaactg gctgttcctg
     7621 ctggtcgggg ccggtgtcgg cggcggcatc ggcgcattgc tggcccagcg catcgcgatg
     7681 acggcaatgc cgcagctggt ggccgcgttc cacagcctcg tcggtcttgc ggcggtgttc
     7741 gtcgcggctg cggcgcttta ttcaccagcg gcgttcggta tcggagcccc gggcgcgatc
     7801 aagggcgcct cgctgttcga gatggcgctg ggtgccgcga tcggtgcggt gaccttcacc
     7861 ggatcggtca tcgccttcct caagctcgac ggtcgcatgt ctggcgcgcc gatcatgctg
     7921 ccgcaacggc atgcgatcaa catcggcctc ggtgtgctgc tggccttttt catcctggtg
     7981 ttcatccgct cggaatcgac ggcggtgttc tggctgatcg tgctggtcag ctttgcgctc
     8041 ggcgtcttgc tgatcatccc gatcggcggc gccgacatgc cggtggtggt ttcgatgctc
     8101 aactcctatt cgggctgggc ggcagcgggc atcgggttca cgcttggcaa tctcgcgctg
     8161 atcatcaccg gcgcgctggt gggctcctcg ggcgcgatcc tcagctacat catgtgcaag
     8221 gcgatgaacc gctcgttctt ctcggtcatc ctcggcggct tcggcggcga ggcggtggcg
     8281 ggcggagcgg cgcaggaagc gcggccctac aaggccggct cggcggatga tgcggcctat
     8341 atcatgaaga atgcctccaa ggtcatcatc gtgccgggat acggtatggc tgtggcgcag
     8401 gcccagcatg cgttgcgcga gatggccgac cttctgaaaa aggaaggggt gcaggtctcc
     8461 tatgccatcc atccggtggc tgggcgcatg ccggggcaca tgaacgtgct gctggccgag
     8521 gccaacgtgc cgtatgacga ggtgttcgag cttgaggaca tcaactcgga attcgcccaa
     8581 gcggatgttg ccttcgtcat cggcgccaac gacgtgacca acccggcggc caagaccgac
     8641 aagacttcgc cgatctatgg catgccggtg ctcgacgtcg agaaggcggc gaatgtgctg
     8701 ttcgtcaaac ggtcaatggg cggcatcggc tatgccggta tcgacaacga cctgttctat
     8761 cgcaacaaca cgatgatgct gctggccgac gccaagaaga tggtcgagaa catcgtgaaa
     8821 gcgctggcgc attaatccga taccataccc tcgaccgggg cgatcagcct ggcgacaccc
     8881 ctgacatgac aaaggccgcc tcgaggcggc ctttttcttt gtcgttccgt tcaaagaccg
     8941 agaatcagcg gaacaggccg aagccgccgc gcgagaccga ggcatccttg ggcaggacga
     9001 cgttcgggtt gagattcagc gcacgctggt aggcctcgtt cgcctcagcc ttgcggccga
     9061 gccggtcaag cgcatagccg cgcccggccc agccgtcggc ctgccggttg tcgatgttca
     9121 gcgtcgcggt gaaatcctcg agcgcctgtt cgaacttgcc gatggcattc aggctctggc
     9181 cgcgacccag atagggcgga gcgacatagg gattgcggtc gatcgccgca tcgaaatcgg
     9241 aaatcgccag cgggtgctgg ccctgcaact gatggatcag cccgcggcca tgataggcct
     9301 cggccatgtc cggcttgagc cgcagcgcct gactgagatc ctcattggcg gactgcatgt
     9361 tgccttgcgc gcggtagagg ttgccacggg cgagataggc cgccggataa ttgggatcgg
     9421 cctggatggc gcggttgaaa tcgccgaaag ccagatcgtt cttgccgatc tggcgatggg
     9481 caagaccgcg attgacgtaa gcagcggcga aattcggatt gatccgcacc gcgcgcgaga
     9541 aatcattgat cgcatcattg aaccgaccgg cccgggcata ggcggcaccg cgcgtgttgt
     9601 aagcctgggg atcatccggg ttgcgggcaa tgacatcgct cagcgagccg atattggccg
     9661 agctggcggc cgcagcgctt gcgtcatcga cttccgcgac gccggccggg ttgatcgggc
     9721 tgatcgtctc gcaggctccc attgccagaa ccagcgccag aaccggcacc agaaccggca
     9781 ctcttgccca ggttcttaca ccgcgagatt gccgcagtgc ggcatgggaa caggaactct
     9841 tcatggctgc agccagcatc acaccactcg tttcgaccgc gcccctcagg ggcactctta
     9901 gcgctgttta gcgaaaacat accacaaaaa cttaaaagcg cccggaatcc ggacgctttt
     9961 ttgcacatga gccaaaggtg gctcccagga ccgcctggcg atccggctgc gcagaaggtc
    10021 tcagaccttc ttgaccttcg gcttggcggc cggcagcagg ccttcgcgct gcagacgctt
    10081 gcgcgtcagc ttgcgggcgc ggcgcacagc ctcggccttt tcgcgagcgc gcttctcgga
    10141 cggcttctcg taatgaccgc gaagcttcat ttcacggaaa atgccttcac gctgcatttt
    10201 cttcttgaga gcgcggagcg cctgatcaac gttattgtcg cgaacgagta cctgcacgtg
    10261 ctgtgtccgt cagtcctggc gtggtgaaaa catacgggca ggcgcctaga tgcctgccag
    10321 cggaaggttg ggtcattatc aaagcgaccg gcgcttgtcc agcgcgggaa aacggcacct
    10381 gtgcggaaaa tgggcggtat tcaaccggcg tgacgccgca cacgtgtcac atggcccatc
    10441 ttgcggcctg gacgcggttc gctcttgcca taaagatgga gatgcgctcc aggctcggcc
    10501 agcagtctgg cccaatcgcc ggcctcgtcg ccgatgagat tctgcatcgt cacgcttgcc
    10561 ccggtgcatg ccaccgatcc gagcggccag ccggcgatag cacggatatg ctgctcgaac
    10621 tgcgatgttt ccgcaccttc gatggtccag tggcccgagt tatgcacgcg aggagcgatc
    10681 tcgttgacga tcaggcgctc gctgccgccg tcacgaagca ggaacatttc caccgcgaat
    10741 accccgacgg catccagccc gtcgccgatc cggcgggcga tgtcgagcgc gcgggcctgc
    10801 gtctcgggcg aaatccgcgc cgggacattg gtctgccaga ggatatggtt gcgatgctcg
    10861 ttctcgcaga cgtcgtaggc ggcgaatgtg ccatcaaggc cgcgggcggc gatcaccgaa
    10921 acttcggcag aaaaggggac aaagccttcc agaatggccg gcgccccgcc gattgcatca
    10981 taggcagccg gaagggcggt atcgggacgg atcaccacct gccccttgcc atcatagccg
    11041 aaacggcggg tcttgagcac gctcggccgg ccaagggcaa gcaccgccac ctcaagttcc
    11101 gtcagcgaat caacgctcca gaacggggcg gtctcgatgc cgagatcgga catgaaggtc
    11161 ttttcgtgca accggtcctg gctgatcgac agcatgcggg cgccggggcg gaccggcttc
    11221 agcggcgcaa gaatctccac cgtgcgggcc ggaacattct cgaactcgta ggtgatgaca
    11281 tcgcagcggg cggcaaagcg ggcgagcgcc tcggcgtcct cataatcccc ggtcatgcat
    11341 tcggtggcca catcgaaggc cgggccctcg cgttcaggct ggtagatcag ggtcctcagg
    11401 ccaagttccg ccgccgccag ggcggccatg cgaccaagct gaccgccgcc gagaatgccg
    11461 atggtctggc gcggaaccag acggttcatg gctcgtccct cgggcgttcg gcgaccgcgg
    11521 cggtctgtgc cgaccgccag ccatcaagcc gggttgcgag cgccggatca tgcagggcaa
    11581 ggacggcagc ggccaaaagc gcagcattga cggcgccagc gcggcctatg gccaatgtgc
    11641 ctaccgggat tccggccggc atctgcacga tcgaatgcag gctgtcgacg ccggacagcg
    11701 ccttggattc aaccggcacg ccgaagaccg gcagcggtgt catcgccgcc gtcatgccgg
    11761 gcagatgggc ggcgccgccg gcgccggcga tgatgacgtg ataaccggcc tcgcgggccg
    11821 atctggcaaa ggaaaaaagc cgctccggcg tgcggtgggc agagacaatg cgcgcgtcaa
    11881 acgcgatgcc cagcgcttcc agtgtgtccg ccgcgtgtct catcgtcgac cagtcggact
    11941 ggctgcccat gatgatggcg actctggcgg gtgttccggt catatccgca tgtcccttag
    12001 atcacgctct cgtttcggct caatcaccga aacgagagat atagtgaccg acaataataa
    12061 cctggagcat gcttttggcg aaaaaccgtc gacacttttt cgcagcatgc tccaaggctg
    12121 ctcccctgta tccaaccatg cggcgccagc gcaaggctct cgtgcgcgtc cggttagagc
    12181 atgctgcgat tgggtgaaag ccggctcatc gcaaaaggca tgctgtgaac tccagatatc
    12241 gatcacgttg cctcgcgctt tgatggaatc atcagaatgc ggataacgtg atcggtatca
    12301 ataagttaga gcatgtcttc tgcgattgac
//
LOCUS       DAOMXG010000061        12308 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_2793_length_12308_cov_9.672325, whole genome shotgun sequence.
ACCESSION   DAOMXG010000061 DAOMXG010000000
VERSION     DAOMXG010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 12308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12308
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2793_length_12308_cov_9.672325"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1467)
                     /locus_tag="PLQ11_05875"
     CDS             complement(<1..1467)
                     /locus_tag="PLQ11_05875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HRK24465.1"
                     /translation="MPHSEKMSPVDTTWLRMDRPNNLMVIVGVLKLAGPVNVDRLEKA
                     IVDRLLVYRRFRQRVETRPAGTWWVEDPDFDPARHIHRIRLPGEGGDPELEHFVGEQA
                     AIPLDHAIPLWQIHIVEGFQGGAALVVRIHHAIADGIALIGVMLSLTDEMHMLSGGGK
                     AAGREAAKEEEEDDHDDWLHQMFAPLGQALKLGGKLSNKGLKYAAEIPSNPGRALDYL
                     REGTSILSELAGLILMSNDSQTPLKGKPHGIKRVAWSEPMPLAEVKALSRGLGCSVND
                     ILLSSVAGAINSYIADRGETTDGVEIRALVPVNLRKEGRQIELGNRFGIVALELPVGI
                     DNPLERVFEVRRRMDQLKKSYEAPVTFGLLSALGYAPKIAQDLLFDTLLSRATAVMTN
                     VPGPQFPMSIAGSQISQIMFWVPQAGDVGIGVSILTYNGKVQFGLITDAALVPDPKAI
                     IAGFRPHFERLLYHTLMEHEGADDEAPAQSYAAARRAEN"
     gene            1550..2284
                     /locus_tag="PLQ11_05880"
     CDS             1550..2284
                     /locus_tag="PLQ11_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24466.1"
                     /translation="MDTKVEIAGRLIARMNDATALRGQARGDEKAMRSRALLRSWQSQ
                     RLARTHADLLADARFHDAAQFFLDELYGLKDTAQRDADATRVAPTLARVLPEAGLETV
                     ADAAELDAISEEFDAAMVRALGRKVEGIDSAGYAAAYRKVGRKDDRLRQIGLVEHLGQ
                     ALDRLTRRAFAGTALKMMRKPAELAGFADLQAFLQTGYDAFRKMGSAEDFLNRIISRE
                     RAIVEALFAGNDAVLDAGPEHFGVSR"
     gene            2287..3717
                     /locus_tag="PLQ11_05885"
     CDS             2287..3717
                     /locus_tag="PLQ11_05885"
                     /EC_number="1.2.1.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050269 - coniferyl-aldehyde dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006081 - cellular aldehyde metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coniferyl aldehyde dehydrogenase"
                     /protein_id="HRK24467.1"
                     /translation="MSSPPPADDRDRLETLFAAQRALSRRVPFPDLSTRRSRLERLLA
                     LLVDNQTAFEQAISADFGHRSVQETRLAEAFVAEAGIRHILSHLKHWMQPRRIKTELA
                     FLPGSNRLMPQPLGAVGIIAPWNYPLQLTINPVAAAFAAGNLVMLKPSEFTPAFSSLL
                     ARLVADLFAPEDFTIIEGGADVARQFADLPFDHLFFTGSTKVGRLVAQAAARNLTPVT
                     LELGGKSPAIIDASADIAKAAGRIAFGKLLNAGQTCIAPDYVLCPPEREAEFISAYKA
                     AVARLYGADAANPDYSAIITDRHFSRLEGLIDDAAARGARIDYAVADPAAWKARRKFP
                     PCLVQGASAEMLVMQEEIFGPILPVLPIADTAAAIAQVNAGDRPLALYWFGTDAAARD
                     RVLAETVSGGVTINDCLVHIAQENQPFGGVGASGMGAYHGEWGFRTFSHDKPIFYRPE
                     LSGLGLMQPPYGKTFDRMLAQLRRII"
     gene            3761..5407
                     /locus_tag="PLQ11_05890"
     CDS             3761..5407
                     /locus_tag="PLQ11_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HRK24468.1"
                     /translation="MLEFDYLVLGGGSAGSVVASRLSERPDVTVALVEAGNKGAGWVV
                     NTPIAGALMVPSRLNNWAFKTVPQKGLNGRIGYQPRGRALGGSSAVNAMVYVRGHRWD
                     YDHWAELGCKGWAFDDVLPYFLKAETNERGASQWHGADGPLHVSNSRTGNPWHAIFLE
                     AAREAQFRMNEDFNGPEQEGFGVYQVTQKNGERWSAARGYLHPFLGKRANLCVETGAQ
                     VTRILFEGRRAIGAEFRQGGVIRHLRARREVILCAGALQSPHVLMLSGVGDAPALQKM
                     GIAPVLNLPAVGKNLQDHPDFIFGYEAKSLDLVGLSMKGFARFFNEIRRYRRERQGML
                     ASNFAEAGGFLKTRSDLPAPDIQVHFVVAMVDDHARRLRWGHGFSAHLCLLRPKSRGS
                     VALAAPDPFTAPLIDPNFLDHPDDVEDMVAGFKQTRRIMDAPALASLRGRDLFTANIR
                     TDEEIRSVLRERVDTVYHPAGTCRMGTGTDAVVTPDLKVVGMEGLRVADVSIMPTLIS
                     GNTNAPAVMIGEKAADLIARTARSADHAPVLMASSKRETT"
     gene            complement(5506..5844)
                     /locus_tag="PLQ11_05895"
     CDS             complement(5506..5844)
                     /locus_tag="PLQ11_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24469.1"
                     /translation="MKRLLRLFSFFSALAGFVLLVIDGTGFVATRVLAVTPLGDVLAR
                     LPAGTFGPLSRERLGALLPEGVWGSLEQAVLSCPAALAFGLAALLFWILGRKPPPDLR
                     LAGRRWQADG"
     gene            6057..6146
                     /locus_tag="PLQ11_05900"
     tRNA            6057..6146
                     /locus_tag="PLQ11_05900"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6091..6093,aa:Ser,seq:gga)
     gene            complement(6147..6506)
                     /locus_tag="PLQ11_05905"
     CDS             complement(6147..6506)
                     /locus_tag="PLQ11_05905"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HRK24470.1"
                     /translation="MVEGPKALVESFYADVWNRRDTTRARKTIASDFRFRGSLGPEKR
                     GVEGFLTYVDAVHAALGDYTCIIKDLIEAPQRVAARMIFRGVHQAEFFGVPAIHQTIE
                     WSGAASANPIAVVRISE"
     gene            6748..7815
                     /locus_tag="PLQ11_05910"
     CDS             6748..7815
                     /locus_tag="PLQ11_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018899557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRK24471.1"
                     /translation="MGGISIEDVSVSYAGNRVIQNLSLTIKDGALFTLLGPSGCGKTT
                     LLRMLAGFLPVDSGRIRFGETDVTQWPPYKRDIGMVFQDYALFPDKTVFDNVAYGLRA
                     RKRDEATIRARTGEYLERVGLSGFEKRRPAELSGGQRQRVALARALVIAPQVLLMDEP
                     LSNLDAKLRVQVRETIRDLQKEAAITTVFVTHDQEEALALSDAIGLMRAGQIEQMGPP
                     VEIYSTPVSAYVADFVGAANVIAATTQAHAGKGADVEVQLASARIVARAVTDVPAGSV
                     QVLARQEDVELHAPDTGSGIPGTVLRRQYLGSRTHYKVQLGVNGPVLAVERTGLAHGS
                     IQEGAAVRVVLDRALTLVVPR"
     gene            7812..9464
                     /locus_tag="PLQ11_05915"
     CDS             7812..9464
                     /locus_tag="PLQ11_05915"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRK24472.1"
                     /translation="MNRRLFDGGIWFWLSLAALVVLGAFILYPLINVLASSFGAARMD
                     GRSGWQILLSEPKYLRAVGNTMLLGLVVTLTTTVIGVPLAYVTARFRFPGKALIAILP
                     LTTLIVPEVIAAQTWLMMLGNNGLITRTLGEWGIPVPSFYGWPGLIIVMTFIYYTYIY
                     MGTLAAIRGFDHQLEQAAQSLGASPGRARLDVMLPVVLPAILASALLVFTLVVGNFAV
                     ATILGHKVELLSVITYQTFVSEIGNDPVMQSTLASVSIGIVALALFLQRFILARGRYE
                     ITLGRGAKPVDIRGVPALALAGAATIVVLISLLPLASVIAGAFTRARGPVMRWGEWTT
                     ANLERVLINAPDPIINTLIYAGAATALSLIVSTGVSYMLVKKRNILTPAIDYLAALPL
                     ALSGTVIGIGLVMSFNQGWLPLTGTAAIIVLAYLIRRLPFGVRNASSTLYNIPNSIEE
                     ASISLGVPPLRSFVAVVLPLMVPAIAAAGVLVWTTTVAELSASIVVYSGGRETLPIQI
                     FRLIDSGLMAQASAYGLVLVSIILIPIIIATALFRLDIFGAK"
     gene            9536..10534
                     /locus_tag="PLQ11_05920"
     CDS             9536..10534
                     /locus_tag="PLQ11_05920"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRK24473.1"
                     /translation="MNMTRRTVLATLASAAAVPAYAQRDQTAGNVVIYTSNNQQSVQA
                     VTDTAASLLPNVKISTITGGSGQLLRRIEAEAGKTQADVFWSSSPNTLGAFKSLYEPY
                     KSPNASAVPAELIEPGTLWTASNIHVVVAMLNTTQLGGHAKPKTWTDLLDPKWKGKII
                     IADPANSSTAYTIIWGVKTLFGSEGLKKLAANVTVTSAAATVLRAVAQGEYAIGLTFE
                     SNAYAYVAGGQKEIELLYPEDGTFTSPESLVLIKNAPAGEAARKVYDLLLSKEAQIAL
                     LEAAFRRPSRTDIDVPKYVKLPNLNTIKIAPIDEAEAARQRTAFLGEWTAAAGPPK"
     gene            10538..11323
                     /locus_tag="PLQ11_05925"
     CDS             10538..11323
                     /locus_tag="PLQ11_05925"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HRK24474.1"
                     /translation="MSELEDCLRAASEFADESRAMLRAAAAETRASELKPDRTFVTAT
                     DRAIELRLRERIADRFPQHGIWGEEFGREKPGAGWQWILDPIDGTAQFIAGIPVYATL
                     IALAKGGVPVLGLMDFPAIGERWIGCEGHATTCNGAICRTSRTMILSDAMMSTSSPDF
                     YAETERPVLAALQGATRWRIYGGAALSYGCLAAGRTDLACDSSFQVYDFAAFRPIIEG
                     AGGVVTDWEGRSLSLDSGSRVLAAATSGLHAEALRLLGQSQRI"
     gene            complement(11347..>12308)
                     /locus_tag="PLQ11_05930"
     CDS             complement(11347..>12308)
                     /locus_tag="PLQ11_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017275255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRK24475.1"
                     /translation="GGAAGLFQRLRVDWRQQRRPEGRVVVDVGTNAEILLACGNRRYA
                     ASSPTGPAFEGAQIRCGQRAAPGAIERVRIDPATLEPRIRIIGSDLWSDEAGFTEAAA
                     KLGVTGLCGSGIIEALGELFLAGVITSEGVIDGTLAALSNRVIPEDRTWSYLLWKGTI
                     EIRITQADVRAIQLAKAALYAGVRLLMEHSGVDRLDRIKLAGAFGTHIDPLYALVIGM
                     VPDCDVTKVTAVGNAAGTGARMALLNRAHRRVVAEIVRSVDKIETALEPRFQALFVDA
                     MAFPNARDAFPCLSQTVRLPTRIHAPHDTPQRRGGRRGRTGTS"
BASE COUNT         2186 a         4000 c         3815 g         2307 t
ORIGIN      
        1 gttctcggcg cgacgggcgg cggcgtagct ctgggccggg gcctcatcgt ccgcgccttc
       61 atgctccatc agggtatggt agagcagccg ctcgaaatgc ggccggaagc cggcgatgat
      121 cgccttggga tccggcacca gcgccgcatc ggtgataagg ccgaactgca ccttgccgtt
      181 ataggtcagg atcgacacac cgattccgac atcgcccgcc tgcggcaccc agaacatgat
      241 ctggctgatc tgcgatccgg cgatcgacat cgggaactgc gggcccggca cattggtcat
      301 caccgcagtc gcgcggctca gaagcgtgtc gaacaaaaga tcctgggcaa tcttgggggc
      361 atagcccagc gccgacagga gcccgaatgt caccggcgcc tcgtaggatt tcttcaactg
      421 atccatccgg cggcggacct cgaacacccg ttcgagcggg ttgtcgatcc ccaccggcaa
      481 ttccagcgca acgatgccga accggttgcc gagttcgatc tgccgcccct ccttgcgcag
      541 attgacagga accagcgcgc ggatctccac cccgtcggtc gtctcgccgc ggtcggcgat
      601 ataggaattg atcgctccgg ccactgacga gagcaggatg tcattgaccg aacagcccag
      661 cccgcggctc agcgccttga cctcggcgag cggcatcggc tcgctccagg ccacccgctt
      721 gatgccgtgc ggcttgccct tgagcggggt ctggctgtca ttggacatca ggatcagccc
      781 cgccagctcg ctcaggatgc tggtgccctc cctcagataa tcgagcgccc tgcccggatt
      841 cgacggaatt tcggccgcat atttcaggcc cttgttcgac agtttgccgc ccagtttgag
      901 cgcctgtcca agcggcgcaa acatctggtg cagccaatcg tcgtggtcgt cctcctcttc
      961 ttccttggcc gcctcgcggc cggccgcctt cccgccgccc gacagcatgt gcatctcatc
     1021 ggtcagcgac agcatgacgc cgatcagcgc gatgccgtcg gcaatggcgt ggtgaatgcg
     1081 cacaaccagc gccgccccgc cctgaaaccc ctcgacgatg tgaatctgcc acagcggaat
     1141 ggcatgatcg agcggaatgg cggcctgttc gccgacaaaa tgctccagct ccgggtcacc
     1201 tccctcccct ggcagacgga tgcggtggat atggcgggcc ggatcgaaat ccggatcctc
     1261 cacccaccat gtcccggccg gccgcgtctc cacccgctgg cgaaaccgtc gatagaccag
     1321 caggcgatcg acgatcgcct tttccagccg gtcaacgttg accgggccgg cgagtttcag
     1381 caccccgaca atcaccatca aattgttcgg ccgatccata cgcagccacg tcgtatcgac
     1441 gggcgacatc ttttcgctat gcggcatcgt cccctggtcc tccccaacgc cgcccaagcc
     1501 aatttgtctt taggcggcgc ttactaatcc gggatggtag agtattttca tggacacgaa
     1561 agtggaaatt gccgggcgcc tgatcgcccg gatgaacgat gcgaccgcgc tgcgcggcca
     1621 ggcgcgcggt gacgaaaagg cgatgcggag ccgtgcattg ctgcgcagct ggcaatcgca
     1681 gcggctggcg cgcacccatg ccgacctgct tgccgatgcg cggtttcacg acgcagcgca
     1741 gtttttcctc gacgaactct acgggctgaa ggacaccgcc cagcgcgatg ccgatgcaac
     1801 gcgcgtggcc cctaccctcg cccgagtgct tcccgaggcc gggctggaaa cggtagccga
     1861 tgcggccgaa cttgatgcga tctccgagga attcgatgca gccatggtca gggcgctcgg
     1921 ccgcaaggtc gagggaatcg attcggccgg ctatgccgcc gcctaccgca aggtcggacg
     1981 caaggatgac cggctgcgcc agatcggtct ggtcgagcat ctcggccagg cgctggatcg
     2041 cctgacccgc cgcgcctttg ccggaacagc gctgaaaatg atgcgcaaac cggcggaact
     2101 tgccgggttt gccgatctcc aggcattcct gcagaccgga tatgacgcct ttcgcaagat
     2161 gggcagcgcc gaggacttcc tcaaccgtat catcagccgc gagcgcgcca tcgtggaagc
     2221 gctgtttgcc ggcaatgatg cggtgcttga tgctggcccc gagcatttcg gagtgtcgcg
     2281 atgaatatgt catccccacc cccggccgat gaccgggacc ggctggaaac tctgttcgcc
     2341 gcccagcgcg cgctgtcgcg gcgcgtgccg tttcctgacc tttcgacccg caggagccgg
     2401 cttgagcggc tgcttgcgct gctcgtcgac aaccagacag cgttcgaaca ggcaatttct
     2461 gccgatttcg gccatcgctc ggtgcaggaa acccggctgg cggaggcctt tgtcgccgag
     2521 gccggcatcc gccatatcct cagccatctc aagcactgga tgcagcccag acggatcaag
     2581 accgagcttg ccttcctgcc cggatcgaac cggctgatgc cgcagccgct gggcgctgtc
     2641 ggcatcatcg cgccatggaa ctacccgttg caactgacca tcaacccggt ggccgccgcc
     2701 tttgccgccg gcaatctggt catgctcaag ccgagcgaat tcacccccgc cttctcgtcc
     2761 ctgctggcgc ggctggtggc cgatctgttc gcgccggaag atttcactat catcgagggc
     2821 ggcgccgacg tggcgcggca atttgccgat ctgccgttcg atcacctgtt tttcaccgga
     2881 tcgaccaagg ttggccggct ggtggcgcag gcggcggcgc gcaatctgac gccggtgacc
     2941 ctcgaactcg gcggcaagtc gcccgccatc atcgacgcct ctgccgacat cgccaaggca
     3001 gcgggccgca tcgccttcgg caagctgctg aacgccgggc agacctgcat cgcccccgat
     3061 tatgtgcttt gtccgccgga gcgcgaggcc gagttcatct ccgcctacaa ggcagccgtc
     3121 gcccgtctct atggcgcgga tgcggccaat cccgactata gcgccattat cacggatcgg
     3181 catttcagcc ggcttgaagg gctgatcgac gatgcagcgg ccaggggcgc gcgtatcgac
     3241 tacgccgtcg ccgatcccgc cgcctggaag gcaaggcgca agttcccgcc ctgcctggtg
     3301 cagggcgcct cggccgagat gctggtcatg caggaggaga tcttcggccc catcctgccg
     3361 gtcctgccca tcgccgatac ggccgctgcc atcgcccaag tcaatgccgg tgaccgcccg
     3421 ctggcgctct actggttcgg caccgatgca gcggcgcgcg accgggtgct ggccgagacg
     3481 gtctccggcg gcgtgacgat caacgattgc ctcgtccata tcgcgcagga aaaccagccc
     3541 ttcggcggcg tcggcgcctc cggcatgggc gcctatcacg gcgagtgggg ctttcgcacc
     3601 ttttcccatg acaagccgat cttctaccgc ccggagcttt cgggcctcgg cctgatgcaa
     3661 ccgccctatg gcaagacctt cgatcggatg ctcgcgcagc tgcggcggat catctgagcg
     3721 cagcctgtga cggctgagga gacaggacat gacccaggac atgttggagt tcgattatct
     3781 ggtgctcggt ggcggctcgg cgggatcggt ggtcgccagt cggctgagcg agcggccgga
     3841 tgtaaccgtc gcgctcgtcg aggccggcaa caagggcgcg ggctgggtgg tcaacacccc
     3901 catcgccggc gcgctgatgg tgccctcccg tctcaacaac tgggcgttca agaccgtgcc
     3961 gcagaagggc ctcaacggcc gcatcggcta tcagccgcgc gggcgtgcac tcggcggttc
     4021 ctcggcggtc aatgcgatgg tctatgtgcg cgggcaccgc tgggattatg accattgggc
     4081 cgaactcggc tgcaagggct gggccttcga tgacgtgctg ccctatttcc tgaaagccga
     4141 gaccaacgag cgcggcgctt cccagtggca tggggcggac gggccgctgc atgtctccaa
     4201 ttcccgcacc ggaaacccct ggcacgcgat cttccttgaa gccgcacgcg aagcgcagtt
     4261 ccgcatgaac gaggatttca acgggcccga gcaggaaggg ttcggggtct atcaggtcac
     4321 ccagaagaac ggcgagcgct ggagcgccgc ccgcggctat ctgcacccct tcctcggcaa
     4381 gcgcgccaat ctctgtgtcg agaccggcgc ccaggtcacc cgcatcctgt tcgaaggtcg
     4441 ccgggccatc ggcgccgagt tccgccaggg cggcgtgatc cgccatctgc gcgcccgccg
     4501 cgaggtcatt ctctgtgccg gggcgctgca atcgccgcac gtgctgatgc tgtcgggcgt
     4561 gggcgacgca cctgccctgc aaaagatggg aattgcgccg gtgctgaacc tgcccgcagt
     4621 cggcaagaac ctccaggacc accctgattt catctttggg tatgaagcaa aaagcctcga
     4681 tctcgtcggt ctttccatga aaggcttcgc gcgatttttt aacgaaattc ggcgctatcg
     4741 gcgggagcgg caggggatgc ttgcctccaa tttcgccgag gcgggcggct ttctcaagac
     4801 ccggtctgac ctgccggcgc cggatatcca ggtgcatttc gttgtcgcca tggtggacga
     4861 tcatgcccgc cggctgcgct gggggcatgg cttttccgcc catctgtgtc tgctgcggcc
     4921 gaaaagccgg ggctcggtcg ccctcgccgc gcccgatccc ttcaccgcgc cgctgatcga
     4981 tcccaacttc ctcgatcatc ctgacgatgt ggaggacatg gttgccgggt tcaagcagac
     5041 gcggcggatc atggacgcgc cggcgctggc ttcgctccgc ggccgcgacc tgttcaccgc
     5101 caacatccgc accgacgagg agattcgctc ggtgctgcgc gaacgggtcg ataccgtcta
     5161 ccacccggcc ggcacctgcc gcatgggcac gggcaccgat gccgtggtca caccggatct
     5221 gaaggttgtc ggcatggagg gcctgcgcgt tgccgatgtg tcgatcatgc cgacgctgat
     5281 cagcggcaat accaacgccc cggcggtgat gatcggcgag aaggcggcgg atctgatcgc
     5341 cagaaccgcc cgctcggcag atcacgctcc cgttttgatg gcctcatcaa aacgagagac
     5401 aacgtgatcg attaaaatag ttcagagcgt gctttccgcg aaaaaccgtt tccacttttc
     5461 cgcagcatgc tctggcggtg gcctgacccg atcgaggcgg cggattcagc cgtcggcctg
     5521 ccagcgtcgc ccggccagcc gcaggtccgg cggcggcttc cgtcccagga tccagaacag
     5581 cagcgcggcc agcccgaaag ccagcgcggc cggacacgac agaaccgcct gctcaagact
     5641 cccccaaacc ccctccggca gcagcgcgcc cagccgctcg cgcgacaaag ggccgaacgt
     5701 gcctgccgga agccgcgcca gcacatcccc gagcggggtc accgccagca cgcgcgtcgc
     5761 gacaaagccg gtgccgtcga tgaccagcag cacgaagccg gcaagggcgc tgaaaaagct
     5821 gaaaaggcgc agcagtcgtt tcattccaga ttccggccga tacggcaccg attccgcgcc
     5881 atcgtaggca cggcggatcg atctaggcaa cggatatgga tggatgcgtc accttagccg
     5941 cacccccgcc accgattgcc cgtttacatt gcgcccgtcc cggcctatga ggcgctggct
     6001 caacagggcg cgggctcccc gcctttcggc tgtcacagcc ggttcgtcct gggcacggag
     6061 aggtggccga gtggttgaag gcgtacgcct ggaacgcgta tatacgggaa accgtatcga
     6121 gggttcgaat ccctctctct ccgccactac tcagaaattc ggacaactgc gatggggttc
     6181 gcggaggccg cgcctgacca ttctattgtt tgatggatcg caggaacgcc gaagaactca
     6241 gcttgatgaa cacccctgaa tatcatccgt gctgccactc gctgcggtgc ctcaatcaaa
     6301 tccttgatga tgcaggtata gtctccgagc gcggcatgca ctgcgtcgac ataggtcagg
     6361 aagccctcga cgcctcgctt ttctgggccc aaagacccgc gaaagcgaaa atcagatgca
     6421 atagtcttgc gagcgcgagt cgtgtcacgc ctgttccaga catcagcata gaagctctcg
     6481 accagtgctt ttggaccttc aaccatgttg gtgtggctac cggcttgatg tggggaccgc
     6541 aaggcactgt catgtgtaga cggctccact gggtcaaggg agtaggttcg ggtttggatc
     6601 gagacaggga tcggtcatgt gtccggcctg ctgccgcgat ttgatgtcag cccggaacag
     6661 gggcgcgagc tggcgcaacc acgcgtgttt cattgaccaa agcgctggtt tgtgctcgcc
     6721 tcttgaacgg acaggaggcg catcagtttg ggtggaattt caatcgagga tgtatctgtt
     6781 tcctacgcgg gtaaccgggt catccagaac ctttcgctga cgatcaagga cggggcgctc
     6841 ttcacgctgc tcgggccttc ggggtgcgga aagaccacat tgctgcgcat gcttgcgggc
     6901 tttctccccg tggacagcgg aaggatccgg ttcggcgaga ccgatgtcac ccagtggcca
     6961 ccgtataagc gcgatatcgg catggtgttt caggactatg cgctgttccc ggacaagacc
     7021 gttttcgaca acgtggccta tggtctccgc gcgaggaaac gggacgaggc caccatccgc
     7081 gcaagaacag gtgaatacct tgaacgggtg gggctttccg gcttcgagaa gcgtcgcccc
     7141 gccgaactgt ccggcggtca acgccaacgg gtcgcgcttg cccgcgcgct ggtcattgcg
     7201 ccgcaagtgc tgctgatgga tgagccgctt tcgaatctgg atgccaagct ccgggtgcag
     7261 gtgcgcgaga ccattcgcga cctccagaag gaggcggcaa tcacgaccgt ctttgtcacg
     7321 catgatcagg aggaagcgct cgcactgtcc gatgcaatcg gcctgatgcg ggctggccag
     7381 attgaacaga tgggtccgcc ggtcgagatc tattccacgc cggtctcagc ctatgtggcc
     7441 gattttgtgg gagcggccaa tgtgatcgcc gccaccacgc aagcccacgc cggcaaggga
     7501 gctgatgtcg aggtgcaact ggccagtgcg cggattgtgg cgcgggccgt gaccgacgtg
     7561 ccggcaggct ctgtccaggt gcttgcacgg caggaagatg ttgaattgca tgcgccggac
     7621 accggcagcg ggatccccgg aaccgtcctc cgccgccagt atctcggcag ccgaacccat
     7681 tacaaggttc agctcggggt caatggcccc gtgctagccg tggagcggac gggtcttgcg
     7741 cacggcagca ttcaggaggg cgcggctgtt cgggtcgtgc tcgacagggc gctcaccctg
     7801 gtggttccgc gatgaaccgc cgactgttcg acggcggtat ctggttctgg ctgtcgctgg
     7861 cggccttggt ggtgctgggc gccttcattc tctatccatt gatcaatgtt ctggcctcca
     7921 gcttcggtgc agcgcgtatg gatgggcgca gtggatggca gattctgctg tccgagccga
     7981 aatacctccg ggctgtcggc aataccatgc tgctggggct tgtggtcacg ctgacaacca
     8041 ccgtgatcgg cgtgccactg gcctatgtca ccgcgcgctt ccgcttcccc ggcaaggcgc
     8101 tgatcgctat cttgccgctg acgacgctga tcgttcccga agtgatcgcg gcacagacct
     8161 ggctgatgat gctgggcaat aacgggctca tcacccgtac gctcggggaa tgggggattc
     8221 cggtacccag cttctacggt tggccgggcc tcatcatcgt gatgaccttc atctattaca
     8281 cctacatcta catgggcacg ctggcggcca ttcgcgggtt tgatcatcaa ctggaacagg
     8341 ccgcacaaag ccttggtgcg tcaccaggtc gggcacggct ggatgtgatg ctgcccgttg
     8401 tccttcccgc cattctggcc agcgccctgc tggtcttcac gctggttgtc ggcaatttcg
     8461 ccgtcgcgac tatcctcggg cacaaggtgg agttgctctc ggtcatcacc taccagacct
     8521 tcgtctccga gatcggcaac gatcccgtta tgcagtccac cctggcctcg gtctcgattg
     8581 gcatcgtcgc gctggccctt ttcctccagc gcttcatcct cgcgcgtggc cgctatgaaa
     8641 tcacgcttgg acgaggcgcc aagccggtgg atatccgggg cgtgcctgcg cttgcgctgg
     8701 ctggcgccgc cacgatcgtt gtcctgatct cgctccttcc gttggcgagt gtgattgcgg
     8761 gcgccttcac ccgggcacga ggaccggtga tgcgctgggg cgaatggacc accgcaaatc
     8821 tcgaacgcgt cctgatcaac gcgcctgatc cgattatcaa tacgctgatc tatgccggcg
     8881 cggcgacggc cttgagcctg atcgtgtcga ccggcgtgag ttacatgctg gtgaagaagc
     8941 gcaacatcct cacgccggcg attgattacc tcgcagccct gcctctggcg ttgtccggca
     9001 cggtcatcgg gattggactt gtgatgtcgt tcaatcaggg ctggctgccg ctgaccggca
     9061 cggcggcgat catcgttctg gcctatctta tccgtcgcct gcctttcggc gtgcgcaatg
     9121 catcctcgac gctctacaat atccccaatt caattgaaga agcctccatc agccttggcg
     9181 tgccgccgct gcgcagcttc gtggccgtgg tcctgccctt gatggtcccg gccatcgcgg
     9241 cagcgggcgt tctcgtctgg acgacaaccg ttgccgagct ttcggcgtcc atcgtggtct
     9301 attccggggg gcgtgagacc ctgccgatcc agattttccg cctgattgat tccggtttga
     9361 tggcgcaggc ctcggcctac ggcctcgttc tggtctcgat catccttata ccgatcatca
     9421 tcgcgacagc actgttccgg ctcgatattt ttggggctaa atgattgcgg gccggaggca
     9481 agtgtctatt ctgggttcgg aagccatcgg gccaagccaa gggagaatga accccatgaa
     9541 catgacgcgt cggaccgtcc tcgcaacgtt ggccagcgcc gctgccgttc cagcctatgc
     9601 ccagcgggac cagactgccg gcaatgtggt gatctatacg tccaacaacc agcaatctgt
     9661 gcaggctgtg accgatactg ccgcctcatt gctgccgaac gtgaagatca gcaccatcac
     9721 cgggggcagc ggccagcttt tgcgccgcat cgaggccgag gccgggaaga cgcaggctga
     9781 tgtcttctgg agctccagcc cgaacacgct tggcgcgttc aagagccttt acgaacctta
     9841 caagtcccct aacgcttcgg ccgtgcctgc ggagctcatc gagccgggca ctctctggac
     9901 cgcctcgaat atccatgtcg tcgtggcgat gctgaacacg acccagctcg gcggccatgc
     9961 caagcccaag acatggacgg atctgctgga cccaaaatgg aagggcaaga tcatcatcgc
    10021 cgatcctgcc aattcctcaa ccgcctacac gatcatctgg ggcgtgaaga cgctgtttgg
    10081 ctccgaaggg ctcaagaaac tggcggcgaa tgtcactgtc accagcgcgg cagcgaccgt
    10141 tctgcgcgct gtggcgcaag gcgaatacgc gatcggactg accttcgaat ccaatgccta
    10201 tgcgtatgtc gccggcgggc agaaggaaat cgaactgctt taccccgagg acggcacctt
    10261 cacgagcccg gaaagcctgg tcctgatcaa gaacgcgcca gccggcgaag cggcccgaaa
    10321 agtctatgac ctgcttctct cgaaggaagc gcagatcgct ttgctggaag cggccttccg
    10381 ccgtcccagc cgcaccgata ttgatgttcc caaatacgtg aaactgccaa acctcaacac
    10441 gatcaagatt gcgccgatcg acgaggcgga agccgcgagg cagcgcaccg ctttccttgg
    10501 cgaatggacg gccgcagccg gcccgccaaa gtaagcgatg tccgaactcg aagactgtct
    10561 tagagcggct tctgaattcg ccgatgagag ccgcgcgatg cttcgcgcgg cagcagccga
    10621 aacaagggcg agcgagctca agcccgatcg caccttcgtc actgcaaccg atcgggccat
    10681 cgaactgcgc ctgcgggaac gcattgccga ccgctttccg cagcatggca tctggggcga
    10741 ggaattcgga cgcgagaagc ccggcgccgg ttggcaatgg atcctggatc ccattgatgg
    10801 cacggcccag ttcatcgcgg ggataccggt ctatgcaacg ctcatcgcgc tggcgaaagg
    10861 gggcgttcct gtgctcggtt tgatggattt ccccgccatt ggagaacgct ggatcggctg
    10921 tgaggggcac gcaacgactt gcaatggtgc tatctgccgt acaagccgca cgatgatatt
    10981 gtctgatgcc atgatgtcca caagcagccc ggatttctac gcggagacag aacgccccgt
    11041 gctggcagcg ttgcaggggg caacacgctg gcggatctat ggcggggcgg cgctgagcta
    11101 tggctgcctc gcagcaggcc ggacagatct cgcgtgcgat tcaagtttcc aagtctatga
    11161 ttttgccgct ttccgcccga tcatcgaagg tgctggcggc gtggtcaccg actgggaagg
    11221 ccgcagtttg tcactcgata gcggatcgcg cgtgctggcg gctgcaacgt ccgggcttca
    11281 cgcggaggcg ttacggcttc tcggccaatc acagcggatc tgattccggc acttcaagag
    11341 gcactgtcag gaggtcccgg ttcgcccgcg gcgcccgccg cgccgctggg gcgtatcatg
    11401 cggagcatgg atcctcgtcg gcagcctgac ggtctgggac agacacggga aggcatcgcg
    11461 ggcattcggg aaggccatcg catcgacgaa cagcgcctga aagcgcggct ccagcgcggt
    11521 ctcgatcttg tcgaccgacc gcacgatctc cgcgaccacg cggcgatggg cgcgattgag
    11581 cagggccatg cgcgcgcccg tgccggcggc gttgccgacc gccgtcacct tcgtcacatc
    11641 gcaatcaggc accatgccga tcaccagcgc atagagcggg tcaatatgcg tgccgaaggc
    11701 gcccgccagc ttgatgcggt cgaggcggtc aacgcctgaa tgctccatca gcagcctgac
    11761 gccggcatag agcgccgcct tggccaattg gattgccctg acatcggcct gcgtgatcct
    11821 gatctcgatc gtccctttcc agaggagata actccacgtc ctgtcctccg ggatgacccg
    11881 gttgctgagc gccgccagcg tgccgtcgat cacaccctcg gaggtgatga ccccggcaag
    11941 gaacagttcg ccgagagcct cgatgatgcc cgagccgcag agtccggtga cgccgagctt
    12001 cgcggcagcc tcggtaaagc cggcctcgtc cgaccacaga tccgagccga tgatgcggat
    12061 ccgtggctcc agggtcgcag gatcgatgcg cacccgctcg atggcgcccg gcgcggcgcg
    12121 ctgcccacag cggatctggg cgccttcgaa ggccgggccc gtcggcgagg acgccgcata
    12181 gcgcctgttg ccgcaggcaa gcaggatttc ggcattggtg ccgacatcaa caaccacccg
    12241 gccttctggt cttcgctgct gccgccaatc gacccgcagc cgctgaaaaa gccccgctgc
    12301 cccccctg
//
LOCUS       DAOMXG010000062        12252 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_2809_length_12252_cov_10.290317, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000062 DAOMXG010000000
VERSION     DAOMXG010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 12252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12252
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2809_length_12252_cov_10.290317"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1638)
                     /locus_tag="PLQ11_05935"
     CDS             complement(<1..1638)
                     /locus_tag="PLQ11_05935"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HRK24476.1"
                     /translation="MRLANSADFEPSPEAGRSFGSIFNELNRKKRWILLPAVVAFICA
                     AAFVNIVRPKYTGEARVFLENRENYFTRPDKDSRGSEQAMDSEGVQSQVQMVMSRDLA
                     LRVIKKLDLASREEFDSVKKEVSTLRRVLSLLGLAKDPLANSPEERVLENYYDRLLVL
                     NVGKSRVISVEFSSRDPELAYKTANAIAEEYIALQRDAKKSTTQGASAWLGQAIEPLR
                     AKVAEAEAKVEAFRAQNGLLIGASNATIATQQLGEMNTQMSNARSMQSDLQAKAKIIR
                     EALKSGRIFETSEVVNNELVRRLLEQRVAMKTQIALEERSLLPGHPRMKELNAQLSDL
                     ESQIRTAAERTARTLENDARVAGARLQSLQSEFEAQKKTSANANEHEVQLRALEREAR
                     ALRENYEQYLTKYRDSLSRDSDAAAPADARIISRAVLPMLPAFPKKVPIILFSTLATM
                     VLAIGIITTRFMFDVDKAPGAAELPPEVFSQPVQQPMAYQPATVQAPEPAFQEPIIRP
                     PPQAEDYPEHEQPRPAAASAAQWQPAADRESTRLNSSH"
     gene            1865..2356
                     /locus_tag="PLQ11_05940"
     CDS             1865..2356
                     /locus_tag="PLQ11_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HRK24477.1"
                     /translation="MPPDVLQASLHEPYMLNTGDKLRVIVFGQDALSNVYAIDAAGRI
                     SMPLIGSVDARGLTTQSLERAIENKLKSGYLREPKVSAEVDTYRPFFILGEVTTSGQY
                     PFVNNMTIQTAVAIAGGYAPRAARTYAEVTRVVNGDLVTVNVPVTTPIRPGDTIVVKE
                     RYF"
     gene            2546..3682
                     /locus_tag="PLQ11_05945"
     CDS             2546..3682
                     /locus_tag="PLQ11_05945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRK24478.1"
                     /translation="MTPLRILFVFRAPVGGLFRQVIDLIRYQSRSGHQIGIICDSLTG
                     GARALELFEELGPMCPLGIHRMPMHRNPHPSDLTNIRAIAAIVREKNVDVLHGHGSKG
                     GLYARIDAAFRRSGPIRAYTPHGGSFNYEPGTLIHSAYMLVERFLERGTDVFTFESQY
                     IANRFYTECGKTDRLVHVTLNGLYPHEFEPRQTIANPTDILFIGELRAAKGIDMLLQA
                     LSRLRQQGKPITTTIIGGGPDEGLLRAMVLDLGIHDQVIFTGPMVGSEGLKRGRIMVV
                     PSRAESLPYIVLEAVGAQVPLITTNVGGIPEIVGPDYDWMIASGDLEGLVRLLHQAID
                     SSADDLAARSLRLATRARSIFDVEKMAADIVVAYREAIEARIRA"
     gene            3748..5244
                     /locus_tag="PLQ11_05950"
     CDS             3748..5244
                     /locus_tag="PLQ11_05950"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HRK24479.1"
                     /translation="MVDPRSSSASEATPAPGLRLAETVAGLTPRSSTSPAVIMVLARL
                     GEALVIMTVGVLAIYLYIPASEPLKGAYWIAVPGAALLTVAMLQMLGLYRISALQAPL
                     VHGPKVAGGVAAVFLLMLAIMFFLKLEGGFSRGVFSLWLAGGLISLLLARIAIAMVVR
                     GLLHSGALERRAVIVGGGDYAEQLIEAFAASPDSGITLCGLFDERNDPRSPDLVAGYP
                     KLGGIDDLVAYARTMRIDLIIFSLPVTAEARILSMLAKLWVLPADIRLAAHMQRLKFR
                     PRNYSYIGSLPMFDIADKPFSEWDLIIKSAFDRIVAALLILLLAPVMATVALAVRLDS
                     RGPVLFRQKRYGFNNQLIEVFKFRSMHVDQLDANASRLVTRGDPRVTRVGRFIRRTSL
                     DELPQLFNVLTGELSLVGPRPHAVHAKAEDRLYDEVVDGYFARHKVKPGITGWAQING
                     WRGETDTAEKIQKRVEHDLYYIENWSVLFDLYILAMTPVALLQAKNAY"
     gene            complement(5278..6513)
                     /locus_tag="PLQ11_05955"
     CDS             complement(5278..6513)
                     /locus_tag="PLQ11_05955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRK24480.1"
                     /translation="MNIVIVLDCAHINGGQAKVALDSALGLKARGHQPVVFASAGPVL
                     PALVEAGIEVVCLGQHELVNDPDKKAAAMRGLWNRAAEVALAELLARQPRGRTVIHVH
                     GYAKALSPSIARAIARSRLPVIYTMHEYFLMCPNGGFYNYREHHHCTLKPLSLACWKT
                     HCDSRSYGFKLWRGARGLITNHVAGMPKVFSNIAYFHGYQRAIIEPHLPKSTRLYEVA
                     NPIEAEDLGPKADPASGDFLFLGRLSTEKGCPLFAEAAARAGITPAFVGDGPAAAELK
                     AKYPNARFYGWADAAGVRKALRAARCLVFPSLWHEGQPLTVLESLALGTPVIAGDGSA
                     GRESVVDGVTGLWFRQADVADLAEKLRLMQDDARVTAMSNAAHRRYWDHPFSLDRHVN
                     RIEEVYADILGRQGMAGAA"
     assembly_gap    6890..6989
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7018..7095)
                     /locus_tag="PLQ11_05960"
     tRNA            complement(7018..7095)
                     /locus_tag="PLQ11_05960"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7058..7060),aa:Pro,seq:ggg)
     gene            7280..8056
                     /locus_tag="PLQ11_05965"
     CDS             7280..8056
                     /locus_tag="PLQ11_05965"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRK24481.1"
                     /translation="MRVVAILAVLLGLLTPARGEVACQAHSAMGGARIERAALAADQV
                     KITFVGHATFLIETPKGVRIATDYNDYVRPRAVPDIATMNRAHDTHFSRSPDPGIAHV
                     LKGWSETSAMARHDLTFQDVRVRNVPTNIRTYEGTERYGNSIFVFEVAGLCIAHLGHL
                     HHTLTPEHLRTIGQLDVVLVPVDGSFTMDQDGMVEVLDTLGARLVIPMHYFGQSTLQR
                     FLDRLGQNYDIERSPVGEVTVSRTSLPAKPKVLVLPGRLF"
     gene            complement(8072..9016)
                     /locus_tag="PLQ11_05970"
     CDS             complement(8072..9016)
                     /locus_tag="PLQ11_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24482.1"
                     /translation="MRLAAVLIALAALLAPAAAQTAADEKAVRVESRSEPVLCAEKDN
                     IYVPLLSPAVKSFRIQALHPSYIGGLIADRWAADWANCDFGGEPPKGQKPPMPERITL
                     YEDLELQVIGLKYHEFWRPANVPVVINGNSFEGLHLVQLWMRAGNSAEEFLVVYPPDG
                     YWRARPLPPQHMKWTAYGSSFMVGPVTMEGRPVVALKELGFDPKTRTFEMSFVKGGEA
                     TLAVTTIDPDKQVLDVTFSGNLPRDVPFAGLRSMYVTEFNNDAARIAWRGRSAPGWGE
                     APIMEFKPFEAVEVWMGRHSYSRHNTSSPDMVFDRFRE"
     gene            complement(9032..9799)
                     /locus_tag="PLQ11_05975"
     CDS             complement(9032..9799)
                     /locus_tag="PLQ11_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018261717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HRK24483.1"
                     /translation="MALTPDQIERLAAFPALARSIAVYHGDPAREAAMQALYARFVKP
                     GDMVFDIGAHVGDRVAAFRALGAKVVALEPQPDCMAALRIIHGADPGVMLFEQACGAA
                     AGRIAMRINSANPTVSTASDAFIAAADGAGGWEGQVWDRTIEVRVTTLDRLIANHGVP
                     AFAKIDVEGFEAEVLAGLSRPLPALSFEFTTIQRDVALAALDRLKDMGRYRFDVALGE
                     SQQMTFGSPVSLRDMADYIATLPHDANSGDIYAILAT"
     gene            complement(9801..11582)
                     /gene="mdoH"
                     /locus_tag="PLQ11_05980"
     CDS             complement(9801..11582)
                     /gene="mdoH"
                     /locus_tag="PLQ11_05980"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HRK24484.1"
                     /translation="MQAFPVSLQREVLTPTGTQSVRELKRRRQIVALLNIATWLMLIA
                     AFIGLMASNGWTLLDTLMLVCFIVGTPWTVLGFWNSIIGLMLLHSPRDAMVRVAPFAD
                     AGDAPDPITIDTAVLMTLRNEDPARAFLRLKTVRQSLEGTGFGGRFAYFVLSDTNDPA
                     VAAAEEAAFAAWKAEVEPGARLIYRRRTDNAGFKAGNVRDFCATWGADFELMLPLDAD
                     SLMDGPTIVRHVRMMQVHSKLGILQSLVVGMPSKSAFARIFQFGMRHGMRAYTMGSAW
                     WIGDCGPFWGHNALVRIKPFFEDCDLPVVPGGPPLGGPVLSHDQVEATLMRRAGYEVR
                     VLPEEGGSWEENPPTMLEFARRDVRWCQGNLQYLKLLDFPGLYPVSRFQLIWAILMFI
                     GIPAWTLLIALAALKPYDSDAANAFPTTAAIVMFVSFLTMSLMPKLCGLADILMTRGG
                     TARYGGTAKFLMSAVIEIVFSFLQGAVSTLRTTIFMVGLLFGASVVWGGQARDAHGIS
                     WSTAFAGLWPQLLFGTIVLGLMAWASPLLALWSLPLTAGYLLAMPFAVWTADPDIGAA
                     TARAGLAMIPEEVETPDVVRIVQQQGD"
     gene            11668..>12252
                     /locus_tag="PLQ11_05985"
     CDS             11668..>12252
                     /locus_tag="PLQ11_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HRK24485.1"
                     /translation="MNSLSAHALWITGPGRAEVRACTLREPRADEALVRTLYSGLSRG
                     TERLVFNAEVPVSEYRRMRAPLMEGDFPFPVKYGYAAVGVVLKGPEALVDRTVFCLHP
                     HQDRFVAPVAMLAPVPDRVPPRRAIFAANMETALNAVWDSGAGPGDRIVVVGAGVVGL
                     LIARLCARMPGTEVTVVDVASERAETARALGCHFA"
BASE COUNT         2216 a         3880 c         3902 g         2154 t
ORIGIN      
        1 gtgactggag ttcagacgtg tgctctcccg atcggcggca ggttgccact gcgcagcgga
       61 agcggcagcc ggtctgggct gctcatgttc gggatagtcc tccgcctgcg gcggcgggcg
      121 gataatcggt tcctgaaagg cgggctcggg agcctggacg gtggccggct gataggccat
      181 cggctgctgc accggctggc tgaacacctc ggggggcagt tcggcggcgc ccggtgcctt
      241 gtcgacatcg aacatgaagc gcgtcgtgat gatgccgatg gcaaggacca tggttgccag
      301 cgtcgagaac aggatgatcg gtaccttctt cgggaaggcc ggcagcatcg gcagcaccgc
      361 gcgcgagatg atgcgcgcat ccgcaggcgc ggccgcatcg gaatcgcgcg acagcgaatc
      421 gcgatacttg gtcagatact gctcgtagtt ctcacgcaaa gcgcgggcct cccgctcgag
      481 cgcccgaagc tgaacctcat gctcgttggc attggcagaa gtcttcttct gcgcctcgaa
      541 ctcggattgc aggctctgca gccgggcacc ggcgacacgg gcatcattct ccagcgtgcg
      601 ggccgtgcgc tcggccgcgg tgcggatctg gctttccaga tcgctcaact gggcgttgag
      661 ttccttcatg cgcggatggc cgggcagcag cgagcgctcc tccagcgcga tctgggtctt
      721 catcgccacg cgctgctcaa gcaggcggcg gaccaattcg ttgttgacga cctcggaggt
      781 ctcgaaaatg cggccggatt tcagcgcctc gcggatgatc ttggccttgg cctgcagatc
      841 cgattgcatc gagcgggcgt tggacatctg ggtgttcatc tcacccagct gctgggtagc
      901 gatggtggcg ttggaggcgc cgatcagcag gccgttctgg gcgcggaatg cctcgacctt
      961 ggcttcggct tcggccacct tggcccgcag cggctcgatg gcctggccaa gccaggcact
     1021 ggccccctgc gtcgtcgatt tcttcgcgtc ccgctgcagg gcgatatatt cctcggcgat
     1081 ggcattggcg gtcttgtagg ccagttcggg atcgcgcgag gaaaactcga cggagatcac
     1141 ccgcgacttg ccgacgttga gaaccagcag ccgatcgtaa taattctcaa gaacgcgttc
     1201 ttccggcgaa ttggccagcg ggtccttggc cagaccgagc agcgacagca ccctgcgcag
     1261 agtgctgacc tccttcttga ccgaatcgaa ctcctcgcgg gaggccagat ccagcttctt
     1321 gatgacccgc agcgcaagat cgcgcgacat gaccatctgc acctggctct gcaccccttc
     1381 ggagtccatc gcctgctcgc tgccgcggga atccttgtca ggccgggtga aatagttttc
     1441 gcggttttcg agaaaaacgc gcgcctcgcc ggtatatttc ggcctaacga tgttgacgaa
     1501 ggctgcagca cagatgaagg caacgacagc cggcagcaga atccaccgct tcttgcggtt
     1561 gagctcattg aaaatgctgc cgaaactcct gccggcttcc ggcgagggtt caaaatcggc
     1621 ggagttcgca agccgcatgc cactggtctc cagacgcaat ctacgcatgg ccagtttcat
     1681 acgaacatgg ttagtgacgc gttaaattcg aaaaatttgt taagtttgga acttgttaac
     1741 cataacgggc gatgctcgcc ggcagttgtc atcctggcga tttgtccttg tgagcatgaa
     1801 acgtcgttcc ctcctgatca gtgctgttgc cgcgctcatg gctgcgggct gcgccccgtc
     1861 ggtcatgccg ccggatgtgc tgcaggccag cctgcacgag ccctatatgc tcaacaccgg
     1921 cgacaagctc agggtgatcg tgttcggcca ggatgccctg tccaacgtct atgcgattga
     1981 tgccgccggg cgcatctcga tgccgctgat cggctcggtg gatgcacgcg ggctgaccac
     2041 gcaatcgctc gagcgggcaa tcgagaacaa gctcaagagc ggctatctgc gcgaaccgaa
     2101 ggtcagcgcc gaggtcgata cctaccggcc gttcttcatt ctcggcgaag tgacgacttc
     2161 cggccagtat ccgtttgtca acaacatgac gatccagacc gcggtggcga tcgccggcgg
     2221 ctatgctcca cgcgccgcgc gcacctatgc cgaggtcacc cgcgtcgtga acggcgatct
     2281 ggtcaccgtc aatgtgcccg tcaccacccc gatccggccg ggcgacacaa tcgtcgtcaa
     2341 ggaacgctac ttctgacccg tagagcatgc tgcgaaaaag tgtggacggt tcttcggaga
     2401 cagcatgctc cagcttatta ttgtcgatca cgttgtcgct cgtttcggtg attcaaccga
     2461 accgaatgcg tgatctggtg gcagttcggc ctgacggcgc agggacacgc cgacagccgg
     2521 ggctgcggtc gcaaccgagg ctgtcatgac cccattgcgc atcctgttcg tgtttcgtgc
     2581 accggtgggc ggattgttcc gccaggtgat cgacctgata cgctaccagt ccagaagcgg
     2641 tcaccagatc ggcatcatct gcgacagcct gacgggcggg gcccgtgcgc tggagctgtt
     2701 cgaggaactc ggaccgatgt gtccgctcgg catccatcgc atgccgatgc atcgcaatcc
     2761 gcatccaagc gatttgacca acatccgggc cattgccgcc atcgtgcgcg aaaagaacgt
     2821 cgatgtgctg catggccatg gctcgaaggg cggtctttat gcccgcatcg atgctgcctt
     2881 ccgccgctcc ggaccgatcc gcgcctacac cccacatggc ggcagcttca actacgagcc
     2941 gggcacgctg atccacagcg cctacatgct ggtcgagcgg tttctcgagc gcggaacgga
     3001 tgtctttacc ttcgagagcc agtatatcgc caaccgtttc tataccgaat gcggcaagac
     3061 cgaccggctg gtgcatgtca cgctcaacgg cctgtatccg cacgagttcg agccgcgcca
     3121 gaccatcgcc aatccgacag acatcctgtt catcggcgaa ttacgcgccg ccaagggcat
     3181 cgacatgctg ctccaggccc tcagccgcct tcggcagcag ggcaagccga tcaccaccac
     3241 aatcatcggc ggcgggcctg acgagggcct gctgcgggcc atggtgctcg acctcggtat
     3301 ccacgatcag gtaatcttca ccgggccgat ggtcggctcg gaggggctga agcgtgggcg
     3361 catcatggtg gtgccttcgc gggcagaatc cctgccctac atcgtgctgg aggcggtggg
     3421 cgcgcaggtg ccgctgatca ccaccaatgt cggcggcatt ccggagattg tcggaccgga
     3481 ttacgactgg atgatcgcct cgggcgatct cgaagggctg gtgcgtctgc tgcaccaggc
     3541 catcgattcc tcggccgatg acctggcggc ccgatccctg cgtctggcca cacgcgcccg
     3601 ctcgatcttc gacgtcgaaa agatggcagc cgacatcgtc gttgcctatc gcgaagccat
     3661 tgaggcccga atacgggctt aactgacgat ttattgccgt ctggcacact cgccagaccg
     3721 aatatgcgca gcgcaagaca ggtagcgatg gttgaccccc gttcctcttc ggcttctgag
     3781 gcaacgcccg cacccggcct gcggctggcc gagacggtgg ccggcctgac cccgcgcagc
     3841 agcacttcgc cggcagtgat catggtgctg gcgcgcctcg gcgaggcact ggtcatcatg
     3901 accgtcggcg tgctggcaat ctatctctac atcccggctt ccgaaccgct gaaaggcgcc
     3961 tactggatcg cagtgccggg ggcagcgctg ctgaccgtcg ccatgcttca gatgctgggc
     4021 ctttaccgca tatcggcgct gcaggccccg ctggtgcatg ggcccaaggt ggccggcggc
     4081 gttgccgccg tgttcctgct gatgctggcg atcatgttct tcctcaagct cgaaggcggg
     4141 ttctcgcgcg gtgtgttctc gctctggctg gcgggagggc tgatcagcct gctgctggcg
     4201 cggatcgcga tcgccatggt ggtgcgcggc ctgctgcata gcggggcact ggaacgtcgc
     4261 gctgtgattg tcggtggcgg cgactatgcc gagcagctca tcgaggcgtt tgccgcatcg
     4321 cccgacagtg gcatcaccct gtgcggcctg ttcgacgagc gcaacgatcc gcgttcgccg
     4381 gatctggtcg cgggctaccc caaactgggc gggatcgacg atctggttgc ctatgcacgg
     4441 acgatgcgca tcgacctgat catcttctcg ctgccggtga ccgcggaggc ccgcattctg
     4501 tcgatgctgg ccaaactgtg ggtgctgcct gccgacatcc ggctggcggc gcatatgcag
     4561 cgcctcaagt tccggccacg caactattcc tacatcggct cgctgccgat gttcgacatc
     4621 gccgacaagc cgttcagcga atgggacctg atcatcaagt cggcattcga ccggatcgtt
     4681 gcggcgctgc tgatcctgct gcttgccccg gtgatggcga cggtggcgct tgctgtccgg
     4741 ctggattcac gcgggccggt gctgttccgc cagaagcgct acggcttcaa caaccagctc
     4801 atcgaagtgt tcaagttccg ctcgatgcat gtcgaccagc tcgatgccaa cgcctcaagg
     4861 ctggtgaccc gtggcgatcc gcgcgtgacc cgcgtcggcc gtttcatccg ccgcacctcg
     4921 ctcgacgaac tgccgcaatt gttcaatgtt ctgacgggcg aactgtcact ggtcggccct
     4981 cgcccgcatg cggtgcatgc caaggccgag gaccggctct acgacgaggt ggtggatggc
     5041 tatttcgccc gccacaaggt caagcccggc atcaccggtt gggcacagat caacggctgg
     5101 cgcggcgaga ccgatacggc cgaaaagatc cagaagcgcg ttgaacacga cctctattac
     5161 atcgagaact ggtcggtgct gttcgatctc tatatcctgg cgatgacgcc ggtggcgctg
     5221 ctccaggcca agaacgccta ttgaagccgc gcctctgccg gctcgcagac gccccgatca
     5281 ggcggccccg gccatgccct gccggccgag aatgtcggca tagacctcct cgatgcggtt
     5341 gacatgccgg tcgagcgaaa agggatgatc ccaatagcgg cgatgggcgg cattcgacat
     5401 ggccgtcacc cgggcgtcgt cctgcatcag gcgcagcttt tcggcgaggt ccgccacgtc
     5461 cgcctggcgg aaccacagtc cggtgacgcc atcgacaacg ctttcgcggc ccgccgaacc
     5521 atcgccggca atcaccggag tgcccagggc cagcgattcc agcacggtca gcggctggcc
     5581 ctcatgccag agcgagggaa acaccaggca gcgggcggca cgcaacgcct tgcgcacacc
     5641 tgcggcatcc gcccagccgt agaaccgggc attggggtat ttcgccttca gctccgcagc
     5701 cgccggcccg tcgccgacaa aggcgggcgt gatcccggca cgcgccgccg cctcggcaaa
     5761 cagcgggcag cccttttcgg tggacagacg gcccagaaaa agaaaatcgc cgctggccgg
     5821 atcggctttc gggccgaggt cctccgcctc gatcgggttg gcaacctcgt agagcctggt
     5881 ggatttcggc aggtgcggct cgatgatcgc gcgctggtag ccgtggaaat aggcgatgtt
     5941 gctgaacacc ttcggcatgc cggcgacatg gttggtgatg aggccacgcg cgccgcgcca
     6001 gagcttgaag ccataggagc gggaatcgca atgggtcttc cagcaggcca gcgacagcgg
     6061 cttgagcgtg cagtgatggt gctcacggta attgtagaag ccgccgttcg gacacatcag
     6121 gaaatattcg tgcatggtgt agatcaccgg cagccgcgaa cgggcgatgg cccgggcgat
     6181 cgacggcgac agcgccttgg catagccgtg gacatggatg acggttctgc ccctcggctg
     6241 gcgggcaagc agctcagcga gcgcaacttc ggccgcgcgg ttccacaggc cacgcatggc
     6301 agcggccttc ttgtcgggat cgttgaccag ttcatgctgc ccgaggcaga cgacctcgat
     6361 gccggcctcg accagcgccg gcagcaccgg cccggccgag gcaaaaacca caggctggtg
     6421 accgcgggcc ttcagaccca gcgcactgtc cagcgccacc ttggcctggc caccgttgat
     6481 atgggcgcaa tcgaggacaa tgacgatgtt catgggcaaa aagtccattc tggcgcaggc
     6541 gccgcacagc cgccgcgcac tatcccgaat agcgcaaaga aatgaacaaa agccgatggc
     6601 aacgcggcca gcgcgattct tacgcctcaa cgaacacgcg cggaacaaag atgtaaacgc
     6661 tgctgtaacc gcgcaaacga aatagaccag cggtatttta aatttgtaat gaaattcaaa
     6721 tttttagaat ggtcggagtg gcgggattcg aacccagatc tcttctgatg gcctctggcc
     6781 aaaggccaac cggccatcag acgtccagtc gtgggttcgt ccgtcagagt gaattggtcg
     6841 gagtggcggg attcgaaccc agatctcttc tgatggcctc tggccaaagn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna gtcgtgggtt cgtccgtcag agtgaattgg
     7021 tcggagtggc gggattcgaa cccacgaccc ctagtccccc agactagtgc gctaaccgga
     7081 ctgcgctaca ctccgacttg agccggctta tagcagcctc agacggcggt gcaatcggtt
     7141 tgagcgatgc cggcccatgc cgatccggca aaggacagcg gaccatccct ccgctcgcgg
     7201 gctgttgatc gggcacggac ttgccggccg gcgccgttgc gcgcaaactc ggacgtcccg
     7261 gctgtctgga gaggcaggca tgcgtgttgt tgcaattctg gccgtgctgc tcgggctgct
     7321 gacaccggcg cgcggcgagg tggcctgcca ggcccacagc gcgatgggcg gcgcccgcat
     7381 cgagcgggcc gcccttgccg ccgatcaggt caagatcacc ttcgtcggcc atgcgacctt
     7441 cctgatcgaa acgccgaagg gcgtgcgcat cgccaccgat tacaacgatt acgtccgccc
     7501 gcgcgccgtg ccggatatcg ccaccatgaa ccgggcgcat gacacgcatt tctcccgctc
     7561 gcccgatccc ggcattgccc atgtgctgaa aggctggagc gagaccagcg ccatggcccg
     7621 gcatgacctg acgtttcagg atgtgcgcgt gcgcaacgtg cccaccaaca tccgcaccta
     7681 cgagggaacg gagcgctacg gcaattcgat cttcgtgttc gaggtggcgg gcctgtgcat
     7741 cgcccatctc ggccatctgc accacacgct gacgccggag catctgagaa ccatcggcca
     7801 gctcgacgtg gtgctggtgc cggtggatgg ctcgttcacg atggatcagg acggaatggt
     7861 cgaggtgctc gacacgttgg gcgcgcgcct tgtcattccg atgcattatt tcggccaatc
     7921 gaccctgcag cgctttctcg accggctcgg ccagaactac gatatcgagc gcagcccggt
     7981 cggcgaggtc accgtgtcgc gcacgagcct gccggcaaag ccgaaggtgc tggtgctgcc
     8041 ggggcggctg ttctgaggcg gcggtccgcc cctattcccg gaaccggtcg aacaccatgt
     8101 cgggcgagga ggtgttgtgg cgcgagtagc tgtggcggcc catccacact tccaccgcct
     8161 cgaatggctt gaactccatg atcggcgcct cgccccagcc gggggcgctc cttccgcgcc
     8221 aggcgatgcg ggcggcgtcg ttgttgaact cggtgacata catcgagcgc aagccggcaa
     8281 acggcacatc gcgcggcaga ttgccggaga aggtcacgtc cagcacctgc ttgtcggggt
     8341 cgatggtggt gaccgccagc gtcgcctcgc cgcccttcac aaagctcatc tcgaaggtcc
     8401 tggtcttggg atcgaagccg agttccttca aggccaccac cgggcgacct tccatcgtca
     8461 ccggcccgac catgaaggac gaaccatagg ccgtccattt catgtgctgc ggcggcaggg
     8521 gccgggcccg ccaatacccg tccggcgggt agacgacgag gaattcctcg gccgaattgc
     8581 cggcgcgcat ccacaattgc accagatgca gcccctcgaa gctgttgccg ttgatgacca
     8641 ccggcacatt cgccggacgc cagaactcgt ggtatttgag gccgatcacc tggagttcaa
     8701 ggtcctcata cagcgtgatg cgctccggca tcggcggctt ctggcccttg ggcggctcgc
     8761 cgccgaaatc gcaattggcc cagtccgccg cccagcgatc ggcaatcaga ccgccgatat
     8821 aggaaggatg cagtgcctgg atgcggaagg atttcaccgc cggcgagagc agcggcacgt
     8881 aaatgttgtc cttttcggca cagagcaccg gttcggagcg gctttccacc cgcacggctt
     8941 tctcgtccgc agcagtctgc gcggcggcag gggcgagcag cgcggcaagg gcgatgagga
     9001 cagcggccag gcgcatgaaa ggctccagcg atcaggtggc aaggatggcg taaatatcgc
     9061 cggaattggc atcgtgtggc agggtagcga tataatcggc catgtcgcgt aatgaaaccg
     9121 ggctgccgaa ggtcatctgc tggctctcgc cgagcgcgac atcaaaccgg tagcggccca
     9181 tgtccttcag ccggtcgagc gctgccagcg ccacatcgcg ctggatcgtg gtgaattcga
     9241 acgacagcgc cggcagcggc cgcgacaggc ccgccagcac ttcagcctcg aacccctcaa
     9301 cgtcgatctt ggcaaaagcc ggcacgccat ggttggcgat cagccggtcg agcgttgtaa
     9361 cccgcacctc gatggtgcga tcccagacct gcccttccca gccgccggcg ccatcggcgg
     9421 cggcgatgaa ggcgtcggag gcggtcgaca ccgtgggatt ggccgaattg atccgcatcg
     9481 cgatgcgccc cgccgccgcg ccgcaggcct gctcgaacag catcacgccg ggatcggcgc
     9541 cgtggatgat gcgcagcgcc gccatgcaat cgggctgcgg ctccagcgcc acgactttcg
     9601 cgcccagagc ccggaaggcg gcaacgcggt cgccgacatg ggcgccgata tcaaaaacca
     9661 tatcgcccgg tttgacaaag cgggcgtaga gcgcctgcat cgccgcctcg cgcgccggat
     9721 cgccatggta aaccgcaatg gagcgcgcca gcgccggaaa ggcggcaagg cgctcgatct
     9781 ggtccggcgt cagcgccatg tcagtctccc tgctgctgca caatgcgcac aacgtccgga
     9841 gtttccacct cttccgggat catcgcaagg ccagcgcgcg ccgttgccgc accgatatcc
     9901 ggatcggccg tccagaccgc aaaaggcatg gcgagcagat agccggccgt cagcggcagc
     9961 gaccacagcg ccagcaaggg cgaggcccag gccatcagcc cgagcacgat ggtgccgaac
    10021 agcaattgcg gccacaggcc ggcaaaggcg gtcgaccagg aaatgccatg cgcatcgcgc
    10081 gcctgcccgc cccagaccac cgaggcgccg aacaacaggc ccaccatgaa gatcgtcgtg
    10141 cgcaaggtgg agaccgcgcc ctggagaaag gaaaagacga tctcgatcac cgcgctcatg
    10201 aggaatttcg ccgttccgcc gtagcgagcg gtgccaccgc gcgtcatcag aatgtcggca
    10261 aggccgcaga gctttggcat cagcgacatg gtgaggaagg aaacgaacat cacgatggcc
    10321 gccgtcgtcg ggaacgcatt ggcggcgtcg ctgtcgtagg gtttgagcgc ggcgagcgcg
    10381 atcagcaagg tccaggccgg aatgccgatg aacatcagga tcgcccagat gagttggaag
    10441 cggctgaccg gatagaggcc cggaaagtcg agcagcttga gatattgcag gttcccctgg
    10501 caccagcgca cgtcgcggcg ggcgaattcg agcatcgtcg gcgggttttc ctcccacgag
    10561 ccgccctctt cgggcagcac gcgcacctcg tagccggcgc ggcgcatcag cgtcgcctcg
    10621 acctgatcgt ggctgagaac aggcccgccc agcggagggc cgccgggcac cacgggaagg
    10681 tcacagtcct cgaaaaacgg tttgatccgc accagcgcgt tatgccccca gaacgggccg
    10741 caatcgccga tccaccaggc ggagcccatc gtgtaggcgc gcatgccatg gcgcatgccg
    10801 aactggaaga tgcgggcaaa ggcgcttttc gacggcatgc cgacgaccag gctttgcaga
    10861 atgccgagct tggaatgcac ctgcatcatc cggacatggc ggacaatggt cggcccatcc
    10921 atcaggctgt cggcatcgag cggcagcatc agctcgaagt cggcgcccca ggtggcacag
    10981 aaatcgcgga cattgccggc cttgaacccg gcattgtcgg tgcggcggcg atagatgagg
    11041 cgggcgcccg gctccacctc ggccttccag gcggcgaagg cggcctcctc ggcggcagcg
    11101 acggccggat cgttggtgtc ggacaggaca aaataggcga agcggccgcc gaaaccggtc
    11161 ccttccagcg actggcgcac ggttttcaac cgcaggaagg cgcgcgccgg gtcctcgttg
    11221 cgcagggtca tcaacacggc agtgtcgatg gtgatcggat cgggcgcatc cccggcatcg
    11281 gcgaagggcg caacgcgcac catcgcatca cgcggcgaat gcaacagcat cagcccgatg
    11341 atcgaattcc agaagccaag caccgtccat ggcgtgccga caatgaagca gaccagcatc
    11401 agcgtatcga gcagcgtcca gccgttgctg gccatcagtc cgatgaaggc ggcaatcagc
    11461 atcagccagg tcgcgatgtt gagcagcgcc acgatctgcc gccgccgctt cagctcgcgc
    11521 accgattgcg tgccggtggg ggtcagcact tcgcgctgca gggaaacagg aaaagcttgc
    11581 acgggatgga atttccggga ttgagttgtt ccctgtggcg atgcccattt tcattgccgg
    11641 atgcaagatg aacacaaggc aaattccgtg aattcgctgt ccgcccatgc tctctggatc
    11701 accggccccg gccgggcgga ggtgcgtgcc tgcaccctga gggagccgcg cgcggatgag
    11761 gcgctggttc gcacgctgta ttccggcctc agccggggca ccgagcggct ggtgttcaat
    11821 gccgaggtgc cggtcagcga ataccggcgg atgcgcgcgc cgctgatgga gggagatttc
    11881 ccgtttccgg tgaaatacgg ctatgcggcg gtcggtgtcg tgctgaaggg gccggaggcg
    11941 ctggtcgacc ggacggtgtt ctgcctgcat ccgcatcagg accggttcgt cgcgccggtg
    12001 gcgatgctgg cgccggtgcc cgatcgcgtg ccgccgcgcc gggcgatctt tgccgccaac
    12061 atggaaaccg cgctcaatgc cgtgtgggat tccggcgcgg gtccgggcga caggatcgtt
    12121 gtcgtcggtg ccggcgtggt cggcctgctg atcgcgcggc tgtgcgcccg catgcccggc
    12181 accgaggtca ccgtggtcga tgtcgccagc gaacgggcgg aaacggcgcg ggcgctgggc
    12241 tgccatttcg cc
//
LOCUS       DAOMXG010000063        12214 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_2818_length_12214_cov_9.144667, whole genome shotgun sequence.
ACCESSION   DAOMXG010000063 DAOMXG010000000
VERSION     DAOMXG010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 12214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12214
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2818_length_12214_cov_9.144667"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="PLQ11_05990"
     CDS             complement(<1..729)
                     /locus_tag="PLQ11_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRK24486.1"
                     /translation="MSSLPGSIDETQALLKSGSYVAERGLATVVFLALKMGRPLLLEG
                     EAGVGKTEIAKVLATTLGRNLIRLQCYEGLDVSSAVYEWNYAGQMMAIRLAEAAGETD
                     RERLGADVFSERHLIKRPLLKALEAGAGGAPVLLIDELDRTDAAFEAYLLEILSDFQV
                     TVPELGTFRAPHPPIVIITSNRTREIHDALKRRCLYHWVDYPDAPRELAILKARYPDV
                     PAKLARQIVHYVQAIRREDLFKAPG"
     gene            complement(798..1853)
                     /locus_tag="PLQ11_05995"
     CDS             complement(798..1853)
                     /locus_tag="PLQ11_05995"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HRK24487.1"
                     /translation="MSRRNFGTFEGRPVDEVMITSEGAEARIIEYGAVLRDLIVPRAD
                     GGRQRVVLGLNSLRDYELHSPHFGAIAGRYANRIGGGTFRLDGWDYQIPKNQAGKHAL
                     HGGGQGFGKRPWTILAHDASSVTLGLVSSDGDAGFPGTLTAACRYVLKGPTLRVELTA
                     FTDKPTICNLTNHSYFKLDDGLDVLDHDLQIRANLYTPIDEDLIPDGTLAPVSGTPFD
                     FRRPRPILMMRDDGERAWYDHNWVLRRDPLEADESGLDLALAARLSSQRSKMQMEVWT
                     TEPCLQFYDAERLNLPVPGLDGAMYGAHAGLCLETQHAPDSPNLPHMPSTVLRPGELY
                     RHVTEFRFGPIGGDDAA"
     gene            complement(1904..3820)
                     /locus_tag="PLQ11_06000"
     CDS             complement(1904..3820)
                     /locus_tag="PLQ11_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HRK24488.1"
                     /translation="MTTTAAPDVSCCTKAAPHDEHGHAHGHDAVPFEPIAIARIGVAL
                     LAIAVLWFEPSPPITMGIGVGVLAFSGWPIFREAFENLVERRMTMELSMTIAIVAAAA
                     IGEFFTAIVVAFFVLVAEELEHLTVQRGRTAIKDLIAFVPAEARVRRGGEIVTLAVSE
                     IVAGDRVLVSPGEKIPVDGTVTDGHSSVDQSRITGESMPAEKTAGAIVYAGSINQVGS
                     LEVAVSRVGRDTTYGRIIEAVEQAEQARAPVQRLADRLAGYLVYFSFAAAIITFVLTR
                     NIRDTISVIIVAGACGIAAGTPLAILGGIGRAAKLGSIIKGGVHLETLGKIDTVVLDK
                     TGTLTEGEPVVERLIAAPGVSDHDLLRITAAAELHSEHPLAMAVLKRAREAGVPISEP
                     ESFSYTLGRGIEARIEGRFVQVGNRKMMQGAGIALPERPADAVGSEILVAVDGAFFGE
                     ILVRDPVRAEARAAMADLAGLGIKTMLLSGDVQVVASSVGAELGIGEAVGDMLPEDKL
                     NRIRAMVAEGRHVAMVGDGVNDAPALTAATLGIAMGSGTDVAKESADVVLIGNDLVKL
                     VETLRIARRTRSIIWQNFAGTIGVDTLGIVLAAFGMLNPLLAALIHVGSELLFLLNSA
                     RLLPAADRLKPTGA"
     gene            complement(3981..7346)
                     /locus_tag="PLQ11_06005"
     CDS             complement(3981..7346)
                     /locus_tag="PLQ11_06005"
                     /inference="COORDINATES: protein motif:HMM:NF024516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3971 domain-containing protein"
                     /protein_id="HRK24489.1"
                     /translation="MAAIVVGMLYFNLERHPLVGQYLRDKLVRVLEERMGAAFKVGLD
                     AVDLRREQAQTTIKVTGFHLRHADGRRIIDAPEGVITLDSARLVLLALVPTRIELSGL
                     SVVLELSETGQLGLGEDESAGVVAAPAVQPKAPEMDAAGPIDRLRTWIGATFASLSAT
                     REALGGTFPQLGIREATFAIQDRRNGQKFSLSGIRADLSTADSSAVLARTEVRMGQTA
                     FAVDLELSPPGEATQRLRVRSERFDAGDILALSGLMAAGVDGRTEVDFLLDAEVDQSR
                     QAVSARAEVSFGRTALNLNASEPPLVLDRSRAVLNWTQQAPGITIESLDITSGAVALK
                     LAGTITPPAAPAGDWSGRLSAAEPRFDGLAQADEVIQLSSLDLDLAYSPATGALRVEN
                     LKAIGPKANVTAAVQIAPDASGQPSVKVDVAATDTDARAALRLWPRFFAPETRRWLAD
                     HVHGGLLTDLVLAVHVPGDVLAAAAARPLPEEAVSARWKLTGTVLQALPDTPLIRDVN
                     STGRATGRSVRVDIEKGMINTGGRKLMLHASQFAIADTWAKVPEALIRARFSGSADAL
                     ADLARAPGLQPFVPRPIDPARIKGQAEGEVTLALKLNDRPQAGDVRIGMNAILKDVSV
                     DTGYAGRALEGGPLQLTVERETILLKGDARLGGIPAQVEVRGSGKAPPMATLNLTIDD
                     AARARLGMSAGALLTGPILARLTVPLHEDGKGNDGKSNDGKSEVAAEFDLARAGIGEI
                     VPGWSKKAGTPGKARAVLAERPGGAWSLSKFELDAGTLSLRGAVDLARDGTFQKASFS
                     SFKLSPGDNVQVDAERSGAITRIAVRGNAFDARPFLKATQTGSIDKANARDTELTLKT
                     TVMSGFGSELVANADLRMEKRGNDLKRFDLSGRFDGGPVTVKLQPQAGAPTLVVESGD
                     AGAFLRFFDLYARMRGGNLILSAALGEGSQRGTILVRDFSLRDEPALKRLVTDQTATA
                     STGGDSTRLPPEVLRRLGQTKDVPFTKMNVTFARTPGRLDVQDAVVWGPEIGGSMNGS
                     LDYLRDRVSLTGTFVPAFSLNNMFAQVPVLGPLLGGGRNEGLFAVRYTISGQLSAPTL
                     TIDPLTAIAPGFLRKIIDFRGHAQQREKAGPQQ"
     gene            7336..7644
                     /locus_tag="PLQ11_06010"
     CDS             7336..7644
                     /locus_tag="PLQ11_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24490.1"
                     /translation="MAAIVASDNTDPMAAAPSTNRQRMAGRAEADAEALTSVAAGKPA
                     PGADPLSPPGIVVASSILIPLYRELRFQARALLAELRGSPYVWLMADACDEPKAGDNP
                     "
     gene            7641..8111
                     /locus_tag="PLQ11_06015"
     CDS             7641..8111
                     /locus_tag="PLQ11_06015"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRK24491.1"
                     /translation="MSQPLTIGAPAPDFTLASDGGGSITLSSLRGKTIVLYFYPKDDT
                     SGCTMEAKDFNALLPKFEAAGAVVVGVSPDPVKSHDAFKKKYGLCFPLAADPDKAMLS
                     AYGVWVEKSMYGRKYMGVERTTVLIDRNGHVARIWPKVKVPGHAAEVLKAAEAL"
     gene            complement(8161..8463)
                     /locus_tag="PLQ11_06020"
     CDS             complement(8161..8463)
                     /locus_tag="PLQ11_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24492.1"
                     /translation="MAAVALAGCQGVPADYKPDPSLKSASVAKLQSKVAEVCVKAQRA
                     KGSVTTGEVSKPCGCYASRALKAMDKSEIEFYRGNGYFADSARPKAQAALEACGLK"
     gene            complement(8556..10640)
                     /locus_tag="PLQ11_06025"
     CDS             complement(8556..10640)
                     /locus_tag="PLQ11_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRK24493.1"
                     /translation="MNAPAKRSAFSTCPHDCPSACSVEVELLDGNRIGRLRGAREQSY
                     TAGVICAKVARYAERIHHPDRLLHPLIRSGPKGSGQFRRASWDEAMERVAEGLLAAER
                     AHGSQAVWPYYYAGTMGFVMRDGINRLRHVKRYSGFYGTVCITPAWTGFIAGTGRLAG
                     VDPREMARADCVVIWGTNPVATQVNVMTHAVRARKERGAKIVAIDIYRTETLEQADLP
                     IIVRPGTDGALAVAVMHVLFRDGLADRAYLSRYTDDPDGLEQHVASRTPAWAEAITGV
                     PAATIEAFARLVGTTQRTFFRLGYGFSRQRNGAVNMHAASCIAAVTGAWQHEGGGVFF
                     NNGAIYRWDKTMIEGLDAVDPAVRVLDQSQIGRVLTGDAEALRHGPPVMALFIQNTNP
                     VSIAPEQSLVRRGFARDDLFTVVHEQFLTETAQMADVVLPATMFLEHDDIYQGGGHQH
                     MMLGPKLIEAPGECRSNHEVIVDLARRVGASHPGFAMSPRALIDDLLARSGRGSFETL
                     KAERWRDLQPDFATAHYRDGFAWPDRKFRFRPDWSSVPLANDGLKGPWQSLPGLPDHA
                     DLIEATDEDHSFRLATSPARSFLNSTFTETPTSMGRERQPSLLIHPADAGENGIADGD
                     AVEIGNRRGTVFLTARLFDGVQRGVVIAESIFPNASHREGRGINTLIGADSIAPYGGA
                     AVHDCAIWLKRA"
     gene            complement(10812..11177)
                     /locus_tag="PLQ11_06030"
     CDS             complement(10812..11177)
                     /locus_tag="PLQ11_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24494.1"
                     /translation="MRSLRSILCAVAVVSAAVPASAECSLSKLMFNFGETTTQYLDVR
                     QGEYCSGDLKMPGVTVFRIEVDKQPKNGLVSVNQKTYVWKYRPFKNFTGQDEMVLRLY
                     GYSSRTNNHGAMVFKIRVL"
     gene            complement(11208..>12214)
                     /locus_tag="PLQ11_06035"
     CDS             complement(11208..>12214)
                     /locus_tag="PLQ11_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24495.1"
                     /translation="TPPPPPSGTQGFLDLLRRVAPQVQPAAIYGDRLPGRASANNPRQ
                     SCWTCVPYCYNYQVPEVICDTALTMLSCGQSIEYGRLNPYAGAAHTACSFSYGGCWLR
                     GTFPKTTLVSGCVSDPNERKVQTNQVSWCPWVPPTAWDKLTPTGSPEPITGPNMNCPT
                     PMLGLSGNRQQIIETLDRMTPVPGGTHNDVGLRWGLRSLSPNSGWPAFFGLTKLPGVF
                     KVDSAKVMILITDGENTQAVDYPGFWGCSGHMNPGCNGSPNQQALDARMLSWCSAIRN
                     QYGVDLYTIAVNFSNPAAVAKLVQCAGDPKKSFSIDAAQLQTVLDLISSRILKLRLTE
                     "
BASE COUNT         2178 a         4137 c         3819 g         2080 t
ORIGIN      
        1 gcccggcgcc ttgaacaggt cctccctgcg gatcgcctgc acataatgga cgatctgccg
       61 ggcgagtttg gccggcacat ccggatatcg cgccttcagg atcgccagtt cgcgcggcgc
      121 atcggggtag tcgacccagt gatagaggca gcggcgcttg agcgcatcgt ggatttcgcg
      181 cgtgcggttc gaggtgatga tgacgatcgg cggatggggc gcgcggaagg tgccgagttc
      241 cggcacggtg acctgaaaat cggagaggat ttccagaaga taggcttcga atgcggcatc
      301 ggtgcggtcg agttcgtcga tcagcagcac tggcgctcca cccgcgcccg cttccagcgc
      361 cttcagcaat ggccgtttga tcaggtgccg ttccgaaaag acatcggcgc caagtcgctc
      421 gcgatcggtc tcgcccgccg cttccgccag ccggatcgcc atcatctggc cggcatagtt
      481 ccactcatag acggccgagg agacatcgag cccctcgtag cattgcaggc ggatcaggtt
      541 tcgtccgagc gttgtcgcca gcactttggc gatctcggtc ttgccgacgc cggcctcgcc
      601 ttcgagcagc agcggccggc ccatcttcag tgccagaaac accaccgtag ccaggccgcg
      661 ctcagcgaca taggagccgg acttcaggag cgcctgcgtc tcgtcgatgc tgccgggcaa
      721 tgaggacatc gggccttgcg cctttcttcg agggggagga gcagacgagc cttgcgcggc
      781 gctgccgtca agaggcgtca ggccgcgtcg tcaccgccaa tcggaccgaa gcggaattcg
      841 gtgacatggc ggtagagttc gccgggccga agcacggtcg agggcatgtg tggcagattg
      901 gggctgtcgg gcgcgtgctg ggtctccaga cagaggcccg catgggcgcc atacatcgcc
      961 ccgtcgagcc cgggcaccgg caggttgagc cgctcggcat cgtagaactg gaggcagggc
     1021 tcggtggtcc acacctccat ctgcatcttc gagcgctgcg acgacagccg cgccgccagc
     1081 gccagatcga gccctgattc atcggcttcg agcggatcgc ggcgcagcac ccagttatgg
     1141 tcataccagg cgcgctcgcc atcatcgcgc atcatcagga tcgggcgcgg cctgcggaaa
     1201 tcgaagggcg tgccggagac cggcgccagc gtaccgtcgg gaatgagatc ctcgtcgatc
     1261 ggcgtgtaga gattggcacg gatctgcaag tcgtggtcga gcacatcgag cccgtcgtcg
     1321 agcttgaaat aggaatggtt ggtgaggttg cagatcgtcg gcttgtcggt aaaggccgtc
     1381 agttcaaccc ggagcgtcgg ccccttgagg acatagcggc aggcggcggt cagcgtgccg
     1441 ggaaagccgg catcgccatc ggacgagacc agccccagcg tcaccgaact tgcatcatgg
     1501 gccaggatgg tccacggccg cttgccgaag ccctgcccgc caccatgcag cgcatgcttg
     1561 ccggcctggt tcttcggaat ctgataatcc cagccgtcca gccggaaggt accgccgccg
     1621 atgcggttcg catagcgccc ggcgatcgcg ccgaaatgcg gcgagtgcaa ttcgtagtca
     1681 cgcagcgaat tgaggccgag cacgacacgc tggcgcccgc catcggcccg cggcacgatg
     1741 agatcgcgca gcacggcgcc atattcgatg atccttgctt ccgccccttc gctggtgatc
     1801 atcacctcgt cgaccggacg cccctcaaag gttccgaaat tgcggcggct catcgcgtca
     1861 ctcctgttgc cgccggcagc gttgaccgga ccgacagcag ccgtcaagca cctgtcggct
     1921 tcaaccggtc ggctgcgggc aacagccgcg ccgagttgag caggaacaac agctccgaac
     1981 cgacatggat cagcgccgcc agcagcggat tgagcatgcc gaacgccgcc agcacgatgc
     2041 cgagcgtatc gacgccgatg gtgccggcga aattctgcca gatgatcgag cgggtccgcc
     2101 gggcgatcct gagtgtctcg accagcttga ccagatcatt gccgatcaga accacatcag
     2161 cgctttcctt ggcgacatcc gtgccggacc ccatggcgat gccgagcgtt gccgccgtca
     2221 gcgccggagc atcgttgacc ccatcgccga ccatcgccac atgccggccc tcggccacca
     2281 tggcacggat gcgattgagc ttgtcctcgg gcagcatgtc gccgaccgct tcgccgattc
     2341 cgagttccgc cccgacgctg ctcgccacca cctgcacatc gcccgacagc agcatggtct
     2401 tgatgccgag cccggcgaga tcggccatcg ccgcccgcgc ttcggcccgc acgggatcgc
     2461 gcaccaggat ttcgccgaaa aacgcgccat cgacagcgac caggatttcc gatcccaccg
     2521 catcggccgg ccgctccggc aaggcgatgc cggcgccctg catcatcttg cggttgccca
     2581 cctgcacgaa gcgcccttcg atccgcgcct cgatgccgcg cccgagcgtg taggaaaagc
     2641 tctccggttc gctgatcggc acgcccgctt cccgggcgcg cttgagcaca gccatcgcca
     2701 gcggatgctc cgaatgcaat tccgccgccg ccgtgatgcg cagcagatcg tgatcgctga
     2761 cgcctggcgc ggcgatcagc cgctcgacca ccggctcgcc ctcggtcagc gtgccggtct
     2821 tgtcgagcac gaccgtgtcg atcttgccca atgtttccag atgcacgccc cccttgatga
     2881 tcgaccccag tttcgccgcc cgtccgatcc cgccgagaat cgccagcggc gttccggctg
     2941 caatgccgca ggcaccggcc acgatgatca ccgagatggt gtcgcggatg ttgcgggtca
     3001 gcacaaaggt gatgatcgcc gcagcgaagg agaaatagac gagatagccg gcgagccggt
     3061 cggccagccg ctgcaccggg gcgcgcgcct gctcagcctg ctcgaccgcc tcgatgatcc
     3121 gcccgtaggt cgtgtcgcgc ccgacccggc tcaccgcgac ttcaagactg ccgacctgat
     3181 tgatcgaacc ggcatagacg atggcgccgg cggtcttttc cgccggcatc gattcaccgg
     3241 tgatgcgcga ttgatcgacg ctcgaatgcc cgtcggtgac cgtgccgtcg accgggatct
     3301 tctcacccgg cgagaccagc acgcgatcgc ccgcgacaat ctcgctcacc gccagcgtga
     3361 cgatctcgcc gccccgccgg acccgcgctt cggccggcac gaaggcgatc aggtccttga
     3421 tcgccgtgcg cccgcgctgc accgtcagat gttccagttc ttcagccacc agaacgaaga
     3481 aggccaccac gatggccgtg aagaactcgc cgatcgccgc cgctgcgaca atcgcgatgg
     3541 tcatcgacaa ttccatcgtc atgcggcgct cgacgaggtt ctcgaacgct tcgcggaaga
     3601 taggccagcc ggaaaacgcc agcacgccca cgccaatccc catcgtgatc ggcggcgatg
     3661 gctcgaacca cagcacggca atggccagca aggcgacgcc gatccgcgcg atcgcgatcg
     3721 gctcgaacgg caccgcgtca tggccgtggg cgtggccgtg ctcgtcatgc ggggccgcct
     3781 tggtgcagca ggagacatcg ggggcggcag tagtggtcat ggaaatgctc cttgtccgga
     3841 gctatacgcc ttccttagaa cccttccaag aaaatattag aacaattcaa aagtgaaaca
     3901 ccgcgacgcc ccatgcggat gcgcgacatt cccccgcgcc gggggcacag ctggccagcc
     3961 cttgcgacgg ctggcgccgg tcactgttgc gggcccgcct tttcccgctg ctgggcatgg
     4021 ccacgaaaat cgatgatctt gcgcagaaag cctggcgcga tggcggtcag cggatcaatc
     4081 gtcagggttg gcgcgctcaa ctggccgctg attgtgtagc gcacggcgaa caaaccctcg
     4141 ttgcggccac cgccaagcag cgggccgagc accggcacct gggcgaacat gttgttgagc
     4201 gaaaaggccg gaacgaaggt gcccgtcagg ctcacccgat cgcgcagata atccagcgac
     4261 ccattcatcg aaccgccgat ctccggcccc cagactaccg catcctgaac atcgagtcgg
     4321 cccggcgtgc gcgcgaaggt gacattcatc ttggtgaagg gcacgtcctt ggtctggccg
     4381 aggcgccgca gcacctccgg cggcaggcgg gtgctgtccc cgccggtgct ggcggtggcg
     4441 gtctgatcgg tgacgagccg cttgagcgcc ggttcgtcgc gcagcgaaaa atcgcgcacc
     4501 agaatggtgc cgcgctggct tccctcgccg agggcggcgg acaggatgag gttgccgccc
     4561 cgcatgcgcg cgtagagatc gaagaagcgc aggaaggccc ctgcatcgcc gctttccacc
     4621 acaagggtcg gcgcccccgc ctgcggttgc agcttgacgg tgaccggccc gccgtcgaac
     4681 cgtccgctca gatcaaagcg tttgaggtca ttgccccgct tttccatgcg aagatcggca
     4741 ttggccacca gttcgctgcc aaacccgctc atcaccgtgg tcttgagggt caattcggta
     4801 tcgcgggcat tggccttgtc gatgctgccg gtctgcgtcg ccttgaggaa cggccgggca
     4861 tcgaaggcat tgccgcgcac ggcgatgcgg gtgatcgcgc cggaccgctc cgcatcgacc
     4921 tgcacattgt cgccgggcga cagcttgaac gaggagaaag aggccttctg gaaggtgccg
     4981 tcgcgcgcca gatccaccgc gccgcgcaac gacagggtcc cggcatcaag ctcgaatttg
     5041 ctgagactcc aggccccgcc cggccgttcg gccagcacag cgcgggcctt gccgggcgtt
     5101 ccggcctttt ttgaccatcc gggcacgatt tcgccgatcc cagcgcgcgc cagatcgaat
     5161 tcggcagcca cttcgctctt gccatcattg ctcttgccat cattgccctt gccatcctca
     5221 tgcagtggca ccgtcaaccg ggccaggatc ggtccggtca acagggcgcc ggcgctcatc
     5281 cccagtcgcg cccgcgctgc atcgtcgatg gtcaggttca gcgtcgccat cggcggtgcc
     5341 ttgcccgaac cgcgcacctc cacctgtgcc ggaatgccgc cgagccgcgc atcacccttc
     5401 aagaggatcg tctcgcgctc gaccgtcagt tggaggggcc ccccttccaa tgcgcgcccg
     5461 gcataaccgg tatcgaccga gacgtccttg agaatggcgt tcatcccgat ccgcacatcg
     5521 ccggcctgcg gtctgtcgtt cagtttcagc gccagggtca cctcgccctc ggcctgcccc
     5581 ttgatccgcg ccggatcgat cggccggggg acaaagggtt gcaggccggg cgcgcgtgcc
     5641 agatcggcca acgcatcggc gcttccggaa aaccttgccc ggatcagtgc ttcgggaacc
     5701 ttcgcccagg tatcggcgat ggcaaactgg ctggcatgca gcatcagctt gcggccacct
     5761 gtgttgatca ttcctttttc tatgtcgacc cgaacgctgc gtccggtggc ccggccggtg
     5821 gaattgacat cgcggatcag cggcgtatcg ggcagcgcct gcaacaccgt tccggtcagt
     5881 ttccagcgcg ccgaaaccgc ctcctccggc aacggcctgg ccgctgccgc cgccagaaca
     5941 tcgcccggaa catggacggc cagcaccaga tcggtcagca acccgccatg cacatggtcg
     6001 gcaagccagc gccgtgtttc cggcgcaaag aatcgcggcc aaagccgcag cgccgcccgc
     6061 gcatcggtgt cggttgctgc aacatcgacc ttcaccgatg gctgcccgga cgcatcaggg
     6121 gcaatctgca cggcggcggt gacattggcc ttggggccga tcgctttgag gttttcgacc
     6181 cgcaacgctc cggtcgcggg gctgtaggcc agatcgagat cgagcgagga aagttgaatg
     6241 acttcatccg cctgggccag tccatcgaac ctgggctcgg cggcagaaag ccgtccgctc
     6301 cagtcgcctg ccggcgctgc cggcggtgtg atcgttccgg ccagcttcag ggccacggca
     6361 cccgaggtaa tgtccaggct ctcgatcgtg attcccggcg cctgctgcgt ccagttgagc
     6421 acggcacggc tgcgatcgag caccagcggc ggttcactcg cattgaggtt gagcgccgtg
     6481 cggccgaagg acacttccgc ccgggccgac acagcctgcc tcgactgatc cacctcggca
     6541 tcgagcagga agtcgacctc cgtgcggcca tcgacgcctg cggccatcag cccggacagg
     6601 gccagaatat cgccggcgtc gaaccgttcg gagcgcacgc gcagccgctg cgtcgcctcg
     6661 cccggcggcg acagttcgag atcgacggca aacgccgtct ggcccatccg cacttcggtg
     6721 cgggccagaa ccgcgctgga atccgccgtc gacagatccg cgcggatccc ggacaggctg
     6781 aatttctgtc cgttgcgccg gtcttggatc gcaaaggtcg cctcgcggat gccaagctgc
     6841 ggaaacgttc cgcccaacgc ctcacgggtt gccgacaggc tggcaaatgt ggcgccgatc
     6901 caggttcgca ggcgatcaat gggcccggct gcgtccatct ccggggcttt tggttgcaca
     6961 gccggggcgg cgacaacgcc ggcgctctcg tcctcgccaa gcccgagttg cccggtctcg
     7021 gacaattcga gcacgacgct gaggccgctg agttcgattc tggtcggcac cagcgcaagc
     7081 aacaccagcc gggcggaatc gagcgtgatg accccttccg gcgcgtcgat gatccgccgc
     7141 ccgtcggcat ggcgcagatg aaaccctgtg accttgatcg tcgtctgcgc ctgttcacgc
     7201 cgcagatcga ccgcatcgag cccgaccttg aacgccgccc ccatccgctc ttcgagcacc
     7261 cgcaccagct tgtcgcgcag gtattgcccg acgagcggat gccgttcgag attgaaatac
     7321 agcatcccga cgacgatggc cgccatcgtg gccagcgata acaccgatcc tatggcagcc
     7381 gcgccaagca caaaccgcca gcgaatggcc ggccgagcag aagcggacgc ggaagccttg
     7441 acttccgtcg ctgcgggcaa gccggcacct ggtgccgacc ccctgtcgcc cccagggatc
     7501 gtcgtcgcct cgtccattct gattccacta taccgggaat tacgttttca ggcacgtgcc
     7561 cttctggcgg agctgcgcgg cagtccctat gtctggctca tggcagacgc ctgtgacgaa
     7621 cccaaggcag gagacaatcc atgagccagc ccctcaccat cggcgccccc gcccccgatt
     7681 tcactctcgc cagtgacggg ggcggcagca tcaccctgtc cagcctcagg ggtaaaacga
     7741 tcgtgcttta tttctacccc aaggacgaca cctcaggctg caccatggag gccaaggatt
     7801 tcaatgcctt gctgccgaaa tttgaagctg cgggcgccgt cgtggtcggc gtttcgcccg
     7861 atccggtcaa gagccatgat gccttcaaga agaaatacgg cctgtgcttt ccgctggccg
     7921 ccgatccgga caaggcgatg ctgtccgcct atggtgtctg ggttgagaaa tcgatgtatg
     7981 gccgcaaata catgggcgtc gaacgcacga ccgtgctgat tgaccgcaac ggccatgtcg
     8041 cccgcatctg gcccaaggtg aaggtccccg gccacgccgc cgaagtgctc aaggcggccg
     8101 aagccttgta ggtccagccg gtctggcgcg gggatgccct cgcgccagat cgaaggccgg
     8161 ttatttcaac ccgcaggcct ccagcgcggc ctgcgccttc ggtcgtgcgc tgtcggcgaa
     8221 atagccgttg ccgcggtaga attcgatctc gctcttgtcc atcgccttca gcgcccggct
     8281 ggcatagcag ccgcagggct tggagacctc tccggtcgtc acgctgccct tggcccgctg
     8341 agccttgacg cagacctccg caaccttgct ctgcagcttg gcgaccgatg ccgatttcag
     8401 cgaaggatcg ggcttgtaat cggccggcac gccctggcag ccagccagtg caaccgcagc
     8461 cacgcaaacg catacaaccc ggcgaactcc agcaaacatt caatcacccc cacattcgtc
     8521 gtgatgtccc attcgccccg ggagcgcccg cttcgtcaag ccctcttgag ccagatcgca
     8581 caatcatgca ccgccgcccc cccgtagggt gcgatggaat cggcgccgat cagcgtattg
     8641 atcccgcgtc cctcgcgatg actggcgttc ggaaagatgg attcggcaat caccacaccg
     8701 cgctgcacgc cgtcgaacag ccgtgcggtc agaaacaccg tgccgcgccg gttgccgatc
     8761 tcgaccgcat cgccgtcggc aattccgttc tccccggcat ccgccggatg gatcagcagc
     8821 gacggctgcc gctcgcggcc catcgacgtc ggcgtttcgg tgaaggtcga attgaggaag
     8881 gaccgcgccg gcgaggttgc cagtcggaac gagtgatcct catctgtcgc ttcgatgagg
     8941 tcggcatgat cgggcagccc aggcaggctc tgccaggggc ccttgagccc gtcattggcc
     9001 aatggcacgc tcgaccagtc gggccggaag cggaatttcc ggtcaggcca ggcaaacccg
     9061 tcacggtaat gggcagtggc aaaatccggc tggagatcac gccagcgctc ggccttcagc
     9121 gtttcgaacg agccgcgtcc ggagcgcgcc agcagatcgt caatgagcgc gcgtgggctc
     9181 atcgcaaagc cggggtggct tgcgccaacc cggcgtgcga gatcgacgat cacctcgtga
     9241 ttggaccggc actcacccgg cgcctcgatc agttttggcc cgagcatcat gtgctggtgg
     9301 ccgccgcctt gatagatgtc gtcgtgttcc agaaacatcg tcgcgggcag caccacatcg
     9361 gccatctgcg ctgtttcggt gaggaactgc tcatggacga cggtgaacag atcgtcacgg
     9421 gcgaagccgc gccgcaccag ggactgttcg ggcgcgatgc tgaccggatt ggtgttctgg
     9481 atgaacagcg ccatcaccgg cggcccgtgg cgcaacgcct cggcatcgcc ggtcagcaca
     9541 cggccgatct gcgactgatc gagcacgcga actgccggat caaccgcatc cagcccctcg
     9601 atcatcgtct tgtcccagcg gtagatggcg ccgttgttga aaaagacgcc cccgccctca
     9661 tgctgccagg cgccggtgac ggcggcgatg caactggcgg catgcatgtt gaccgctccg
     9721 ttgcgctggc gcgaaaagcc atagccgaga cggaagaacg tgcgctgcgt cgtccccacc
     9781 agccgcgcga aagcttcgat cgtggcggcc ggcacgccgg tgatcgcctc cgcccaggcg
     9841 ggcgtgcgac tggcaacatg ctgttccagc ccgtccggat catcggtgta gcgtgacaga
     9901 taggctcggt cggcaaggcc atcgcggaac agcacatgca tgacggcaac cgccagcgcg
     9961 ccgtccgttc ccggccggac gatgatcggc agatccgcct gttcgagcgt ttcggtgcga
    10021 tagatgtcga tggcgacgat ctttgccccg cgctccttcc gcgcccgcac ggcatgggtc
    10081 atcacattga cctgggttgc gaccggattt gttccccaga tcaccacgca atcggccctg
    10141 gccatttcgc gcggatcgac cccggccagc cttcccgttc cagcgatgaa gcccgtccag
    10201 gctggcgtga tgcagaccgt accgtaaaac cccgaatagc gtttgacgtg ccgcagccgg
    10261 ttgatgccgt cgcgcatcac gaaccccatg gtcccggcgt aataatacgg ccacaccgct
    10321 tgcgaaccat gggcacgctc agccgccagc aggccctcgg ccacccgctc catggcctcg
    10381 tcccacgagg cgcggcggaa ctggccagag cctttcgggc cggagcggat caacggatgc
    10441 agcagccggt cgggatggtg gatccgctcg gcatagcggg cgaccttggc gcagatcacc
    10501 ccggcggtat agctctgctc gcgcgcaccg cgcaggcggc cgatgcggtt gccgtccagc
    10561 aattcgacct ccaccgaaca ggcggaaggg caatcatgcg gacaggtgga aaaagccgag
    10621 cgtttggccg gagcgttcat gccggaaaca gtggcagcaa tcgccgggag tggcaacatg
    10681 ggaaggcaac gggaagcatg gcagcgctcc gctttgtccc ccgcgaccgc agaaagcccc
    10741 ggcgcccgcc ccgcctgccc cacccctcgc ccgtaaaacc gggcgcccgg gtgtcaaaca
    10801 ggcggatcca gctatagaac gcggatcttg aaaaccatcg cgccgtggtt gttggtgcgg
    10861 ctgctgtagc catagagccg caacaccatc tcgtcctggc cggtaaagtt cttgaagggc
    10921 cgatacttcc agacataggt tttctggttg acgctgacga ggccgttctt cggctgtttg
    10981 tcgacctcga tgcggaacac ggtcacgccc ggcatcttga gatcgcccga gcaatattca
    11041 ccctggcgca catcgagata ctgcgtggtg gtctcgccga agttgaacat cagcttgctc
    11101 agcgagcact cggcgctggc cggcaccgcg gctgaaacca cggcgacggc acacaggatt
    11161 gaacgaaggg atcgcatgat cagtccctgc ctttccggcc tcagccatca ttcggtaagg
    11221 cgcagcttga ggatgcgcga agagatcagg tccagcacgg tctgcagctg tgcagcatcg
    11281 atgctgaatg atttcttcgg atcgcctgcg cactgcacca gcttggcgac cgccgccgga
    11341 ttcgagaaat tgaccgcgat cgtatagaga tcgacgccat actgattgcg gatcgcgctg
    11401 caccaggaaa gcatgcgcgc atccagggcc tgctggttgg gcgaaccgtt gcatcccggg
    11461 ttcatatggc ccgaacagcc ccagaagccc ggatagtcga cagcctgcgt gttctccccg
    11521 tcggtgatca ggatcatcac cttggcggaa tcgaccttga acacaccggg tagcttggtc
    11581 aggccgaaga acgccggcca gccgctgttg ggcgacaggc tgcgcaggcc ccagcgcaag
    11641 ccaacgtcat tatgcgtgcc gccgggcacc ggcgtcatgc gatccagcgt ttcgatgatc
    11701 tgctgccggt tgccggagag ccccagcatc ggcgtcgggc agttcatgtt cggtccggtg
    11761 atcggctcgg gggagccggt gggagtcagc ttgtcccacg cagtcggtgg aacccacggg
    11821 caccacgaca cctggttggt ctgcaccttg cgctcgttcg gatcggaaac gcagcccgag
    11881 accagcgttg tcttggggaa ggttccacgc agccagcaac cgccatagct gaaggagcac
    11941 gcagtatgcg ccgcccctgc atagggattg aggcgaccat actcgatact ctgcccacag
    12001 ctcagcatgg tcagcgccgt atcgcagatc acttccggca cctggtagtt gtagcaatag
    12061 ggcacgcacg tccagcacga ttgccgcggg ttgttggccg aggcccgtcc cggcagcctg
    12121 tcgccataga tcgcggcagg ctggacctgc ggcgcaacgc ggcgcagcag atcaaggaac
    12181 ccctgcgtgc cgctcggcgg cggcggcggc gtgc
//
LOCUS       DAOMXG010000064        12196 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_2825_length_12196_cov_8.777695, whole genome shotgun sequence.
ACCESSION   DAOMXG010000064 DAOMXG010000000
VERSION     DAOMXG010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 12196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12196
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2825_length_12196_cov_8.777695"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="PLQ11_06040"
     CDS             complement(<1..261)
                     /locus_tag="PLQ11_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24496.1"
                     /translation="MIDVTDDKALQAWLKKQPLEIVWLIGARAAWRVWPGLYYSYNQL
                     NEIAATAIVLPLFRGSASAPVASTCPTSADGRRAAAAAVAEAA"
     gene            complement(617..1150)
                     /gene="nusG"
                     /locus_tag="PLQ11_06045"
     CDS             complement(617..1150)
                     /gene="nusG"
                     /locus_tag="PLQ11_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HRK24497.1"
                     /translation="MAKRWYIVHAFSNFEKKVADSIREGAEQRGLSHLFDEILVPTEK
                     VTEVRRGRKIETDRKFFPGYVLVKVDLTDDAYHLIKNTPKVTGFLGADKKPIPIPDHE
                     AERIKGQVVDSSAAPKSAITFKVGEQVRVSDGPFASFNGVVEDVDAIRSRLKVAVSIF
                     GRATPVELEFGQVEKMK"
     gene            complement(1178..1369)
                     /gene="secE"
                     /locus_tag="PLQ11_06050"
     CDS             complement(1178..1369)
                     /gene="secE"
                     /locus_tag="PLQ11_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014130852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HRK24498.1"
                     /translation="MSKLNPLEFLQEVRSEAKKVTWPTQKETMVTTGMVLLMVFVASL
                     FFLFADWIIRILVGLVLSR"
     gene            1624..2799
                     /gene="rnd"
                     /locus_tag="PLQ11_06055"
     CDS             1624..2799
                     /gene="rnd"
                     /locus_tag="PLQ11_06055"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011926302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HRK24499.1"
                     /translation="MPLLTTTDALDAACERLGSHDFITVDTEFLRETTYYPKLCLIQM
                     ASSDEVVLVDPLAPDLSLEPFYRLMANPSVVKVFHAARQDVEIIWTGGRLIPAPLFDT
                     QIAAMVCGFGEQVSYESIAAALANARIDKSSRFTDWSRRPLTEAQLAYAAADVTHLRT
                     VYSAIITKLAKNGRRDWLVDEMAALADPSSYETQPEHAWERLKTRVRKPRELAALIEL
                     AAWREREAQARDVPRGRILKDDALGEIASALPATVEDLGRLRTIPKGFERSRTGADIL
                     AAVERAKNRDTETLPALERPRGGSSNGALIQLLKVLLQAVAERQRVAARLIASSDDIE
                     AIATEEAPDGPVMQGWRRELFGDLALRLKRGEITLGISGGRIVTLPVPDHAALQPAQ"
     gene            complement(2806..4080)
                     /locus_tag="PLQ11_06060"
     CDS             complement(2806..4080)
                     /locus_tag="PLQ11_06060"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRK24500.1"
                     /translation="MFEQPVVSLRSCDLLALALKLTVGPALLIVAGLGGAALWAGPDV
                     FDLVGAIALWVLAVCAAAAYIGRRILDTSERDMAALAITARMLADGRINVEIGGQDRT
                     DCVGDLARALAFLRHRLHKESAAATAAVVEAPAKPATAVSNVLASRTALGAMLVDAGA
                     VNSSVRETARVLGEQVKRAIGDAHAAREASNQGAIAVSSLAAAVDQIAGAVRSISAQA
                     SESTDLVRKASNAGATAIDHVSQLSQAVQRIGRVVTSIRAIAEQTNLLALNATIEASR
                     AGEAGRGFAVVAAEVKALATETARATAEITSLVSRIQDVTTAASAATRGIGQQLEAVD
                     EASRVIAAAVSEQEISTNEIARNSTNAARHSLTAHEHFTAIEAAIIAAGAAVTSLEGA
                     TQRFNETTGRIQAEIDGLIDTVGLPEPVARAA"
     gene            complement(4221..5738)
                     /locus_tag="PLQ11_06065"
     CDS             complement(4221..5738)
                     /locus_tag="PLQ11_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HRK24501.1"
                     /translation="MLATLFSGPNDNMDRPVGVVDIGSNSVRLVVYDALCRTPTPIFN
                     EKSLCGLGRDVALTGRLPEDGVEQALSAMKRFRALADVIGVGTLHALATAAARDASNG
                     PEFIAEAERILRGPIQLISGEREAQLAALGVISGIHKPDGIVADLGGGSLELIDVKDG
                     VLGAGVSLKLGGLALRDLSGRSLKKASKIVQGAMDDLPALRSGTGRDIFAIGGTWRAI
                     ARLHMRHIGYPLNVLHDYAMPADEAIDFCRMVTRVDPESLTAISSVAVDRRPLLAYGA
                     LVLDHLMKRSRARRLIVSALGVREGLLYELLDNAERAKDPLLMVAGELNKLRSRSPAY
                     TVELAGWTDGLFAHPDFSESEAERRLRLAACLLSDISWRAHPDYRGEQSLNVIAHAVL
                     PGVDHAERVFLALSVYYRHIGPVSGETPPRIRELAPTRMIERARLLGAAMRTAYLATA
                     AMPGVLKRVPIRIEDGALVLTLPQDLADLANERLFNRLKQLAKLIGREPVITIAR"
     gene            complement(5747..6823)
                     /gene="purM"
                     /locus_tag="PLQ11_06070"
     CDS             complement(5747..6823)
                     /gene="purM"
                     /locus_tag="PLQ11_06070"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008128463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HRK24502.1"
                     /translation="MSGVRKNGLTYADAGVDIDAGNALVEAIKPLVRATRRTGADAEI
                     GGFGGLFDLKACGFKDPVLVAANDGVGTKLKIAIDAEIHDTVGIDLVAMSVNDLVVQG
                     AEPLFFLDYFATGRLSPKIGVKIVGGIAKGCRLAGCALIGGETAEMPGLYHDRDYDLA
                     GFAVGAVERDAILPRLRAIRPGDVLVGLASSGVHSNGYSLVRRIVRQADLGWDVPAPF
                     APKKTLARALLEPTRIYVKPLLSLLRGPHGGAVKALAHITGGGFIDNIPRVLPKGIGV
                     DLDLGAIQPPKVFGWLAEAGGVSVDEMLRTFNCGVGMVAVVAPEAVEAVLAALGASGE
                     RARKIGTLIADKDRRVQPRGTLAL"
     gene            6943..7473
                     /locus_tag="PLQ11_06075"
     CDS             6943..7473
                     /locus_tag="PLQ11_06075"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRK24503.1"
                     /translation="MNLANWLTLLRLCLVPLVIVAITQHRFDLALIGFVVAGLTDAVD
                     GWIARRFNQMSELGALLDPIADKSLLVSIFITLGILGIIPLWLVVLVVARDLMIVGAV
                     IISWLLDNPVEIRPLLVSKANTAAQLVFAAYKLATLAFQISWPQIDLGLTAVVCLLTT
                     VSIAAYLSGWLRHMTQ"
     gene            7473..8177
                     /locus_tag="PLQ11_06080"
     CDS             7473..8177
                     /locus_tag="PLQ11_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020091805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HRK24504.1"
                     /translation="MARPVRPRPPRPPAQLPLDLALAPQFGEEDFLVGPSNEAAHDFM
                     TLWPDWPARIALLTGPAGSGKTHLAHIWSARSGARLLAASRIGDVSPAMLTDAPALAI
                     DGCDTGTFDEARLFHIINLVHERGRTLLLTARRQPDLWGIATADLLSRLRLAPATAIA
                     GPDDGLLRAVMVKQFLDRQLTVDVSVVEFVVARIERSFAAVRSVVDALDREALALGRR
                     ITRPVAAAVLERIAPS"
     gene            8252..10420
                     /locus_tag="PLQ11_06085"
     CDS             8252..10420
                     /locus_tag="PLQ11_06085"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003598025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HRK24505.1"
                     /translation="MNELARPGRQAATPVALRNQPERFINRELSWLGFNRRVLEEAGN
                     PAHPLLEQLRFLSISASNLDEFFMVRVSGLVEQVIARVETLSDDGLSPAQQLQKIHES
                     VKQLTDAQQQRWHELRRLIAENHIDVLGPDALSEEDRTWLEGYFLSAIFPTLTPLAID
                     PAHPFPFIPNLGFSLALMLSRKSDKSKLRALIRMPQKIERFVRLPAPQGRHRVILVEQ
                     VIILFIGKLFPGYKLKGHGCFRIIRDSDVEIEEEAEDLVRVFESMLKRRRRGVVIRLE
                     VDAAMPQGLRDFLTDEQDLAHGEVTVIDGMIGLNELSQLVDLPRPDLKFVPYVPRFPE
                     RIKELGGDMFTAIRQKDLVVHHPYESFDSVIQFLTQAVRDPNVVAIKQTLYRTSADSP
                     IVRALAEAAEAGKSVTALVELKARFDEEANIRWARDLERAGVQVVYGFIELKTHAKLS
                     LVVRREHGQLVTYCHIGSGNYHPITARIYTDLSVFTADPVFGRDVARIFNFITGYAEP
                     AELEQMSVSPYGVKPRLLKHIEEEIAHVKEGRPGAIWLKCNSLVDPVIIDALYDASAA
                     GVKIDLVVRGICCLRPGIPGLSENITVKSIVGRFLEHARIYAFGCGFGLPHPKAAVYL
                     SSADLMPRNLDRRVEVLLPLTNATVHEQVLEQILYGNLIDNQQSWTVLPDGSSRRITP
                     ADGETPFNAHQYFMTNPSLSGRGKSLKHSSPKDLIRLRPG"
     gene            10479..11111
                     /gene="purN"
                     /locus_tag="PLQ11_06090"
     CDS             10479..11111
                     /gene="purN"
                     /locus_tag="PLQ11_06090"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HRK24506.1"
                     /translation="MLISGRGSNMAALIEAAGLADYPAEIVLVLSNKAEAGGLAIAAA
                     AGIATSVIPHKGYKDRESFDRAMTAAIEASGAELVCLAGFMRLLSPWFCRHWHGRLIN
                     IHPALLPSFKGLDTHARALAAGVKIHGATVHFVIPEMDEGPIIAQGAVPVLPEDDEPA
                     LAARVLGVEHRLYPEALARVATGAARMVEGRCVMAPHGPAQGALMVPSQD"
     gene            11251..12063
                     /locus_tag="PLQ11_06095"
     CDS             11251..12063
                     /locus_tag="PLQ11_06095"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRK24507.1"
                     /translation="MRVRLFALLLSLITPPALAQDRLTRPLSQPPGYSWAGFYFGGHL
                     GYQRSPSGGNLSGYTGYAAGFFETGGLKRFNLDPNGLFGGVQLGANAQFDRLVVGVEA
                     DASLLGHIAKGQSSATNRTTLATLSVTHTETLTSKTDINWLATFRARLGYAVLDRLLI
                     YGTAGLAMSQIRASAGQVSVIHTVPLSGPSTTATNLANAASSSWTRTGWAAGFGAEWA
                     FSRSLSLRGEYLHYSLGDQTLVVQNQTGPAQTTSFRFRNSGDLFRIGLNSLF"
BASE COUNT         2096 a         4162 c         3777 g         2161 t
ORIGIN      
        1 ggcggcttcg gcgacggcgg cggcggcggc gcgtcgaccg tccgcactgg ttgggcatgt
       61 gctcgcaacc ggtgcggacg cactgccacg aaacaacggc agcacgatgg cagtagctgc
      121 gatttcgttc agttggttgt aagaataata aagccccggc catacccgcc aggcggcgcg
      181 ggcgccgatc agccagacga tctcaagcgg ctgctttttc agccacgctt gaagcgcttt
      241 gtcgtcggtg acatcgatca cgcgcgcccc cgggttcccg gtgcatgata tggtgaccgc
      301 atcgcgcctg cgcaagcact tccctcccca aagtggggag ggaggaatgg ggcactctcc
      361 ctcccctgtc aggggagggt ccgagcaaag cgaggggggg gggtgaacgt gccgacagaa
      421 aagttggagc acgccccacc cggtcgctgg cgcgaccacc ctccccaaag tggggaggga
      481 aatctggagc actctccctc ccctcgatgg ggagggtcgc gagtgaaacg agcggggtgg
      541 ggtggcgcgg acaaaccacc cccacccggt cgctggcgcg accaccctcc ccgcaagggg
      601 gagggattcg cgcgccttac ttcatcttct cgacctggcc gaattccagc tcgaccggcg
      661 tggcgcggcc gaagatcgac accgccacct tgaggcgcga gcggatcgca tcgacatcct
      721 cgacgacgcc gttgaacgag gcgaacggcc cgtccgacac gcgcacctgc tcgcccacct
      781 tgaaggtgat cgccgatttc ggcgcggcac tggaatcgac cacctggccc ttgatgcgct
      841 cggcctcgtg atccgggatc gggatcggct tcttgtcggc gccgagaaag ccggtgacct
      901 tcggcgtgtt cttgatcagg tgataggcat cgtcggtcag gtccacctta accagcacat
      961 agccggggaa gaacttgcgg tccgtctcga tcttgcggcc gcgccgcacc tcggtcacct
     1021 tctcggtcgg cacgaggatt tcatcgaaca gatgactcag gccgcgctgc tcggcccctt
     1081 cgcggatcga atccgccacc ttcttctcga aattggagaa tgcgtgaacg atataccagc
     1141 gcttggccat gggggtatcc gatcgaaaag acgggtatca gcgggaaaga accaggccga
     1201 ccaggatgcg gatgatccag tcggcaaaca ggaagaacag cgaggcgaca aacaccatca
     1261 gcagaaccat gccggtggtc accatggttt ccttctgcgt cggccaggtc accttcttgg
     1321 cttccgagcg gacttcctgc aggaactcga ggggattgag cttggacatg caacacccgg
     1381 actttggctg ccctgttgca cccaagtgac cgcgacccgc cgcgcgggga gatcatcaga
     1441 ctgcactgtc agggaatggc agcgtctgta accttttgcg cggcttctgg caagggggcg
     1501 ggcgagcccg tgccgtgttg aaaaaccgtt ttgcactttc cccggcctgg tctagggagc
     1561 gcaggcttgc ctgtccctct ggctggcctt tccgggctgt ccccggcaca tcaaggacca
     1621 tggatgccgc tgctgaccac caccgacgcg cttgacgccg cctgcgagcg tcttggcagc
     1681 cacgacttca tcaccgtcga caccgagttt ctgcgcgaaa ccacctatta tcccaagctg
     1741 tgcctgatcc agatggccag cagcgatgaa gtggtgctgg tcgatccgct cgccccggac
     1801 ctgtcgctgg agcccttcta ccggctgatg gccaacccgt cggtggtgaa ggtgtttcat
     1861 gccgcccgcc aggatgtcga gatcatctgg accggggggc ggctgatccc cgccccgctg
     1921 ttcgacaccc agatcgccgc gatggtctgc ggcttcggcg agcaggtctc ctatgaaagc
     1981 atcgcggccg ccctcgccaa tgcccgcatc gacaagtcat cccgcttcac cgactggtcg
     2041 cgccgcccgc tgaccgaggc gcagcttgcc tatgccgcgg ccgatgtcac ccatctgcgc
     2101 acggtctatt cggcgatcat caccaaactg gccaagaacg gccggcgcga ctggctggtc
     2161 gacgagatgg ccgccctggc cgatccgtca agctacgaga cccagcccga acacgcctgg
     2221 gaacggctga aaacccgcgt ccgcaagccc agggagctcg ccgcgctgat cgagcttgcc
     2281 gcctggcgcg agcgcgaggc gcaggcccgc gatgtgccgc gcggccgcat cctcaaggat
     2341 gatgccctgg gtgaaatcgc ctccgctctg ccggcaacgg tcgaggatct cggccggttg
     2401 cgcaccattc ccaagggttt cgagcgctcc cgcaccggcg ccgacattct cgctgccgtg
     2461 gagcgggcga aaaaccgcga caccgagacc ctgcccgccc ttgagcggcc gcgcggcggt
     2521 tcgtccaacg gcgcgctgat ccagctgttg aaagtgctgt tgcaggccgt ggccgagcgc
     2581 cagcgcgttg ccgcaaggtt gatcgccagt tccgatgaca tcgaggcgat tgccaccgaa
     2641 gaggcgccgg atggtccggt gatgcagggc tggcgacgcg aattgttcgg cgatctcgcc
     2701 ctgcggctga agcgcgggga aatcacgctt ggcatttccg gcggccgcat cgtcaccctg
     2761 ccggtgcccg accacgccgc gctgcaaccg gcgcagtgac cgccctcagg cggcgcgggc
     2821 aaccggctct ggcagtccga ccgtatcgat cagcccgtcg atctccgcct ggatgcgccc
     2881 ggtcgtttcg ttgaaccgct gggtggcccc ttccaggctg gtgaccgccg cgccggcggc
     2941 gatgatcgct gcctcgatgg cggtgaagtg ctcatgcgcc gtcaggctgt gccgcgccgc
     3001 attggtcgaa ttgcgggcaa tctcgttggt ggaaatttcc tgctcgctca ccgccgccgc
     3061 gatcacccgg ctcgcttcat cgaccgcctc aagctgctgg ccgatgcccc gcgttgccgc
     3121 gctggctgcg gtcgtgacgt cctgaatgcg cgaaaccagc gacgtgattt ccgcagtcgc
     3181 ccgcgcggtt tcggtggcga gcgccttgac ctcggccgcg accaccgcaa agccacgacc
     3241 ggcttcgccg gcacgcgacg cctcgatcgt tgcgttaagg gccagcagat tggtctgctc
     3301 ggcaatggcg cggatactgg tcaccaccct gccgatgcgc tgcacggcct gcgacaactg
     3361 ggagacatga tcgatggctg tggcgccggc attggatgcc ttgcgcacca gatcggtcga
     3421 ttcgctggcc tgcgcgctga tgctgcgcac ggccccggca atctggtcaa ccgccgcagc
     3481 aaggctggaa acggcgatcg cgccctgatt ggacgcctcg cgcgccgcat gggcatcgcc
     3541 tatggcccgt ttgacctgtt cgcccagcac ccgggccgtc tcccgcacgc tggaattgac
     3601 cgctccggca tcaacgagca tcgctccaag cgccgtgcgc gaggcaagga cattggagac
     3661 cgccgtggca ggcttggccg gcgcctccac cacggcagcc gtggcagcgg ccgattcctt
     3721 gtgcaaccga tgacgcagga aggccaatgc gcgtgcaagg tcgccgacac agtcggtgcg
     3781 atcctgcccg ccgatctcga cattgatccg tccatcggcc agcatgcgcg ccgtgatcgc
     3841 caatgccgcc atgtcacgct cgctggtgtc caggatgcgg cgcccgatat aggctgcggc
     3901 ggcacagacc gccagaaccc agagcgcgat cgcgccgaca agatcgaaca catccggacc
     3961 ggcccaaagc gctgcaccac cgaggccggc cacgatcagc aacgccggac ctacggtcaa
     4021 cttgagggca agggccagca gatcgcacga gcgcagcgaa acaacgggct gttcaaacac
     4081 gggagcctcc tgggagcatt gcttaaccag aaggcaacag gaatggttaa agcgggctta
     4141 aatgccggaa tttccggtat tttctacctg gatttcagtt cttcaaccaa tatccgtccc
     4201 aaaatgaacc agcatccagc ctaacgtgca atggtaatca ccggttccct gccaatcagc
     4261 ttggcgagct gcttcagcct gttgaacagc cgttcgttgg cgagatcggc cagatcttgc
     4321 ggcaaggtca gaaccagcgc gccatcctcg atcctgatcg gcacccgttt gagcacaccc
     4381 ggcatcgctg ccgtggcgag ataggcggtg cgcatcgccg cccccagcag ccgcgcccgc
     4441 tcgatcatcc gggtcggcgc aagctcgcgg atacgcggcg gcgtctcccc cgagaccggg
     4501 ccgatatggc ggtaatagac cgacagcgcc agaaacaccc gctccgcatg atcgaccccg
     4561 ggaagaaccg catgggcgat gacgttgagg ctctgttccc cgcgatagtc gggatgggcc
     4621 cgccaggaaa tatccgacag aaggcaggcg gccaatctca gccgccgctc ggcctcgctc
     4681 tcgctgaaat ccggatgggc gaacagcccg tcggtccatc cggcaagctc gaccgtatag
     4741 gccggcgaac gcgacctgag cttgttgagt tcgcccgcca ccatcagcaa cggatccttg
     4801 gcgcgctcgg cattgtcgag caattcgtag agcagccctt cgcgcacgcc gagcgccgag
     4861 acgatcagcc gccgcgccct tgaccgcttc atgagatggt cgagcaccag cgcgccatag
     4921 gccagcagtg gccgccgatc gaccgccacc gacgaaatcg ccgtcaggct ttccggatca
     4981 acccgcgtca ccatgcggca gaagtcgatc gcctcatcgg ccggcatggc ataatcgtgc
     5041 agcacattca gcggataacc gatatggcgc atgtgcagcc tggcgatggc ccgccaagtg
     5101 ccgccgatgg cgaagatgtc gcgccctgtc ccgctcctga gcgccggcag atcgtccatc
     5161 gcgccctgca cgatcttcga agcctttttg agcgagcgac cggagagatc gcgcagggcc
     5221 agcccgccga gtttgaggct caccccggcc ccaagcacac cgtccttcac atcgatgagt
     5281 tcgaggctgc cgccaccgag atcggcgacg attccatccg gcttgtgaat ccccgagatc
     5341 acacccagcg cggcaagctg tgcctcccgc tcgccggaaa tgagctggat cggcccgcgc
     5401 agaatccgct ccgcctcggc gatgaattca gggccattgc tggcgtcgcg ggcagctgcc
     5461 gtggccagtg catggagcgt tccgaccccg atcacatcgg ccagcgcgcg aaaccgcttc
     5521 atggccgaaa gcgcctgctc gacgccatcc tccggcagcc ggccggtcag tgccacatcg
     5581 cggccgaggc cgcacaacga cttttcattg aagatgggcg tcggcgtccg gcacagggcg
     5641 tcatagacca ccagacgcac cgagttggac ccgatgtcga cgacaccgac gggccggtcc
     5701 atgttgtcgt tggggccgga gaacagggtc gcgagcatcg agggcctcag agcgcgaggg
     5761 ttccgcgtgg ctggacgcgc cggtccttgt cggcgatcag ggtgccgatc ttgcgggccc
     5821 gttcaccgga tgcccccagc gccgcgagca cggcttcgac cgcctccggc gccacgacgg
     5881 ccaccatgcc gacgccgcag ttgaaggtgc gcagcatctc atcaaccgac acgccgcccg
     5941 cttccgccag ccagccgaac actttcggcg gctggatcgc gccgagatcg aggtcgacgc
     6001 cgataccctt gggcaggacg cgcggaatat tgtcgatgaa tccgccgccg gtgatatggg
     6061 ccagcgcctt caccgccccg ccgtgcggac cgcgcagcag ggacagcagc ggtttgacat
     6121 agatgcgggt aggctcgagc agcgcccgcg ccagcgtctt tttcggcgcg aacggcgccg
     6181 gcacgtccca gccgagatcc gcctgccgga caatccgccg caccagcgaa tagccattgg
     6241 aatgcacccc ggacgaggcg agtccgacaa gcacatcgcc cggccggatc gctctcaagc
     6301 gcggcaggat ggcatcgcgc tccacggcac cgacggcaaa gccggccagg tcataatccc
     6361 tgtcgtgata gagccccggc atttccgccg tctcgccgcc gatcagcgca catccggcga
     6421 gcctgcaccc cttggcgatg ccgccgacga tcttcacccc gatcttcggg ctgagccggc
     6481 cagtggcgaa ataatcgagg aaaaacaggg gttccgcccc ctgcaccacc agatcgttga
     6541 cactcatggc aacaagatcg atgccgacgg tatcgtgaat ctcggcgtcg atggcgattt
     6601 tcagcttggt gccgacgcca tcattggccg ccaccagcac cgggtccttg aaaccgcagg
     6661 ccttcagatc aaacaggccg ccgaagccgc cgatttcagc atccgctccg gtgcggcggg
     6721 tcgcacgcac cagcggcttg atggcttcca ccaaggcgtt cccggcgtcg atatccacgc
     6781 cggcatccgc ataggtcagg ccgttcttgc gcacaccgct catgcgcatt ccggtattga
     6841 ccctcggcct gtttggcaag ccccgcctgt gtatagcggg gccggcaccc cgccgacacc
     6901 tgtgcaaccg caaccggaat acctataaaa aacgccatga gcatgaacct cgccaactgg
     6961 ctgaccctgc tgcggctgtg ccttgtgccg ctggtcatcg tcgccatcac ccagcatcgc
     7021 tttgatctgg cgctgatcgg ctttgtggtg gctggcctga ccgacgctgt cgatggctgg
     7081 attgcccgcc gcttcaatca gatgtcggaa ctcggcgccc tgctcgatcc gattgccgac
     7141 aagtcgctgc tggtgtcgat tttcatcacc cttggcattc tcggcatcat tccgctgtgg
     7201 ctggtcgtgc tggtggtggc gcgcgacctg atgatcgtcg gcgcggtcat catttcctgg
     7261 ctgctcgaca atccggtcga gatccgtccg ctgctggtct ccaaagccaa taccgccgcg
     7321 caactggtgt ttgccgccta caagctggcc acgcttgcgt tccagatttc ctggccgcag
     7381 atcgatctcg gtctcaccgc cgtcgtctgc ctgctgacca cggtttcgat cgcggcctat
     7441 ctgtccggct ggctccggca catgacacag tgatggcacg tcctgtaagg ccacgcccac
     7501 cgcgcccgcc ggcccagctg ccgctcgatc tggcgctggc gccgcaattc ggtgaggagg
     7561 attttctcgt cggtccgtcg aacgaggcgg cgcatgattt catgacgctc tggccggact
     7621 ggccggcgcg catcgccctg ctgaccggcc cggcgggcag cggcaagacc caccttgccc
     7681 atatctggag cgcccgcagc ggcgcccgcc tgctggcggc ttcccggatc ggcgacgtct
     7741 cgcccgccat gctgaccgat gcgccggcac tggcgattga cggctgcgac accgggacat
     7801 tcgacgaggc gcgactgttc cacatcatca atctggtgca cgagcgcggc cgcaccctgc
     7861 tgctgaccgc ccggcggcaa ccggacctct ggggaatcgc aaccgccgac ctgctgtcgc
     7921 gtctgcggct ggcaccggcg acggccattg ccggacccga cgatggtctg ctgcgtgccg
     7981 tcatggtcaa gcagttcctc gatcggcagc tcacggtgga cgtgtctgtc gtcgaatttg
     8041 tcgtagcccg gatcgagcgc tccttcgcgg ccgtccgttc ggttgtcgat gcgctggacc
     8101 gcgaggcgct ggcgctcggt cggcgcatca cccggcctgt ggcggcagcc gtgctggagc
     8161 ggatcgcccc gtcctgacgc gccatgattt cggctgaaat ctctgccgtg atgcgatagc
     8221 ttgatctcat cgtgagcagg atcgccgaac catgaacgaa cttgcccggc cgggccgtca
     8281 ggcggctacc cccgttgcgc tgcgtaatca gccagaacgc ttcatcaatc gcgagctgtc
     8341 gtggctcggg ttcaaccggc gtgtgctgga ggaagcgggc aatccggccc acccgctctt
     8401 ggagcagttg cgattcctgt cgatctcggc gagcaacctc gacgaattct tcatggtgcg
     8461 ggtgtccggc ctggtcgagc aggtgatcgc ccgggtggag accctttcgg atgacgggct
     8521 gtcgcctgcc cagcagctgc agaaaatcca tgaatcggtc aagcagctga ccgatgccca
     8581 gcagcagcgc tggcacgagc tgcgccgcct gattgccgag aaccacattg atgttctcgg
     8641 ccccgatgcc ctgagcgagg aggaccggac ctggctggag ggctatttcc tctcggccat
     8701 cttcccgacg ctgaccccgc tggcgatcga cccggcgcac ccgttcccgt tcatcccgaa
     8761 cctggggttc tcgctggcgc tgatgctgtc gcgcaagtcc gacaagtcca agctgcgggc
     8821 gctgattcgc atgccgcaga agatcgaacg tttcgtccgc ctgcctgccc cgcaggggcg
     8881 tcaccgcgtc attctggtcg aacaggtgat catcctgttc atcggcaagc tgttccccgg
     8941 ctacaagctc aagggccacg gctgtttccg catcatccgc gactcggacg tcgagatcga
     9001 ggaggaggcc gaggatctgg tgcgggtgtt tgaatcgatg ctcaagcgtc gccgccgcgg
     9061 cgtggtgatc cgccttgagg tcgatgccgc gatgccgcag ggtttgcgcg actttcttac
     9121 cgatgagcag gatctggcgc atggcgaggt gacggtcatc gacggcatga tcggcctcaa
     9181 cgaactgtcg cagctcgtcg atctgccgcg gccggacctg aaattcgtcc cttacgttcc
     9241 gcgctttccc gagcgcatca aggaactcgg cggcgacatg ttcaccgcca tccgtcagaa
     9301 ggacctggtg gtccaccacc cgtatgaatc ctttgacagc gtcatccagt tcctcaccca
     9361 ggcggtgcgc gatcccaatg tcgtggcgat caagcagacg ctctaccgca cctcggccga
     9421 ttccccgatc gtgcgcgccc ttgccgaagc ggccgaggcc ggcaaatccg tcaccgcgct
     9481 ggttgaactg aaggcccgct tcgacgagga ggccaacatc cgctgggcgc gcgatctcga
     9541 acgggccggc gtacaggtgg tttacggctt tatcgaactg aaaacccacg ccaagctctc
     9601 gctggtggtg cggcgcgagc atggccagct ggtgacctac tgccacatcg gttcgggcaa
     9661 ttatcatccg attaccgcga ggatttatac cgatctttcg gttttcaccg ccgatccggt
     9721 gtttggccgc gatgttgcgc gcatcttcaa tttcatcacc ggctatgccg aacccgccga
     9781 actcgagcag atgtcggtct cgccctacgg ggtcaagccg cgccttctca agcacatcga
     9841 ggaggaaatc gcccatgtga aggaagggcg ccccggcgcc atctggctga aatgcaattc
     9901 gctggtcgat ccggtgatca ttgatgcgct ctatgatgcc agcgcggcgg gcgtgaagat
     9961 cgatctggtg gtgcgcggca tctgctgcct caggccgggc attccgggat tgtcggagaa
    10021 catcaccgtc aaatcgatcg tcggccggtt ccttgaacat gcgcgcatct atgcgtttgg
    10081 ctgcggcttc ggcctgccgc atcccaaggc ggcggtctat ctatcctcgg ccgatctgat
    10141 gccgcgcaat ctcgatcgcc gcgtcgaggt tctgctgccg ctgaccaacg cgacggtgca
    10201 cgaacaggtg cttgagcaga tcctttacgg caacctgatc gacaaccagc agagctggac
    10261 ggtcttgccc gatggctcat cccggcgcat aaccccggcc gacggcgaga cgccgttcaa
    10321 cgcccatcag tatttcatga ccaacccgag tctctccggc cgtggcaaat ccctcaagca
    10381 ttccagcccc aaggacctca tccgcctccg gccgggatga ggcaccggcc cgcaagggag
    10441 agaccgggtc gcaggcccgc cggcgcgcga aagtcgcggt gctgatctcg gggcgcggct
    10501 cgaacatggc ggcgctcatc gaggcggcgg ggcttgcaga ctatccggcc gaaatcgtgc
    10561 tggttctttc caacaaggcc gaggccggcg ggctggcaat cgctgctgcc gcaggcatcg
    10621 cgacgtcggt gatcccgcac aagggataca aggatcgcga gagcttcgac cgggcgatga
    10681 ccgccgcgat cgaagcctcc ggcgccgagc tcgtctgcct tgcgggcttc atgcggctgc
    10741 tgtcgccgtg gttctgccgc cactggcacg gccgtctgat caacatccac cctgccctgc
    10801 tgccgagctt caaggggctc gacacccacg cccgggcgct cgccgccggc gtcaagatcc
    10861 acggcgcgac cgtgcatttc gtcattccgg agatggatga ggggccgatc atcgcgcagg
    10921 gcgcggtgcc ggttctgccg gaggatgacg agcccgcgct cgcagcgcgc gtgctgggag
    10981 tcgagcaccg gctctacccc gaggccttgg cgcgggtggc gactggcgca gcgcgaatgg
    11041 tcgaaggccg ctgcgtgatg gccccgcatg gcccggcgca gggcgccctc atggtgccat
    11101 cgcaggattg atggccccgg ccatcggcgc ggctgccgcg caaccgatca tttacgacga
    11161 tctcaaagtc tgccgccgcc gggcctcagg ctgaaggatt ggttacggca tcaggcgcaa
    11221 tttgaaggac ctcatccgga gttcccgaca ttgcgtgtcc gcctttttgc cctcctcctc
    11281 agcctgatca cgcctcccgc cctggctcag gaccggctga cgcgcccttt gagccagccg
    11341 ccgggctatt cctgggctgg gttctatttc ggcggccatc tgggctacca gcgctcgccc
    11401 tcgggcggca atctttccgg ctacaccggc tatgcggccg ggttcttcga gaccggcggg
    11461 ctgaagcgct ttaatcttga ccccaacggc ctgttcggcg gcgtgcaact gggcgccaat
    11521 gcgcaattcg accgcctcgt tgtcggcgtc gaagccgacg cgagccttct gggccacatc
    11581 gccaaggggc aatcctctgc caccaaccga acgacgctgg cgacgctatc ggtcacccac
    11641 accgagacac tgaccagcaa gaccgacatc aactggctgg ccaccttccg cgcccgcctg
    11701 ggctatgcgg tcctcgaccg gctgctgatc tatggcactg cgggcctggc catgtcgcag
    11761 atccgcgcct ccgccggcca ggtctcggtc atccataccg tgccgctctc cgggccgagc
    11821 accaccgcca ccaatctggc caatgctgcc tcgtcgagct ggacccgcac cggctgggct
    11881 gccggcttcg gcgcagaatg ggccttctcc cgctcgctgt cgctgcgcgg cgagtatctg
    11941 cattacagcc tcggcgatca gacactcgtc gtgcagaacc agaccggccc ggcgcagacc
    12001 accagcttcc gcttccgcaa ttccggggac ctgttccgca tcgggctcaa ttcgctgttc
    12061 tgataccagt ggccctcaca accgggcctt cggccctgct cggcgtcgca ttcgctcctg
    12121 accagccgcc tgaaaattgc ccctagatca cgttatccgc attttgatgg aatcatcaaa
    12181 atgcggataa cgtgat
//
LOCUS       DAOMXG010000065        12114 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_2848_length_12114_cov_10.044365, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000065 DAOMXG010000000
VERSION     DAOMXG010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 12114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12114
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2848_length_12114_cov_10.044365"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="PLQ11_06100"
     CDS             <1..450
                     /locus_tag="PLQ11_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRK24508.1"
                     /translation="DALQAIFLAHYEKHIADFSRPYPGLIAQLDRLQADGHRLAVCTN
                     KLEAYARLLLDELGLTHRFAAITGGDSFAFKKPDRRHLEETIRLAGSGPAIMVGDSRT
                     DVDTARNAGIPVIGVSFGYTDVPMKDLRPDVLIEHFDELAGAIARID"
     gene            complement(569..643)
                     /locus_tag="PLQ11_06105"
     tRNA            complement(569..643)
                     /locus_tag="PLQ11_06105"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(609..611),aa:Val,seq:cac)
     gene            856..1431
                     /locus_tag="PLQ11_06110"
     CDS             856..1431
                     /locus_tag="PLQ11_06110"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019903000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRK24509.1"
                     /translation="MRRLNTAVFALLLGAVAAPAAVAYEIDPLTREPIIPYQDTRAKA
                     TPIPREEVSYASKFAPGTIVVSTVERRLYYILGNGRAVRYGVGVGRPGFEWGGSKAIT
                     RKAEWPDWTPPPQMLKRRPDLPRHMKGGPENPLGARALYLGSSLYRIHGSNEPETIGQ
                     AVSSGCIRMLNDDVVDLYSRVKVGTRVVVLR"
     gene            1607..2611
                     /gene="moaA"
                     /locus_tag="PLQ11_06115"
     CDS             1607..2611
                     /gene="moaA"
                     /locus_tag="PLQ11_06115"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HRK24510.1"
                     /translation="MSPAPLTDPFGRQVSYLRVSVTDRCDFRCTYCMSEHMTFLPKKD
                     LLSLEELSRLCGLFVQRGVRKLRITGGEPLVRRDIMRLFRDLSQHLANGALDELTLTT
                     NGSQLARFADELADCGVKRINVSLDTLDADKFRSITRWGDLDQVLDGIRAAQAAGLKI
                     KINTVALRDFNDHESLELIRWAHGQGMDITFIEVMPLGEMEVARVDQYLPLSQLRERL
                     GQQLTLKAIDYRTGGPARYMRASETGGRIGFITPMTHNFCESCNRVRLTCTGTLYMCL
                     GQEDAADLRQPLRSSEGDEAVLRAIDDAIARKPRGHDFIIDRDTRQPAVRRHMSLTGG
                     "
     gene            2638..3549
                     /locus_tag="PLQ11_06120"
     CDS             2638..3549
                     /locus_tag="PLQ11_06120"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRK24511.1"
                     /translation="MFSNLSPNGRGILAMTGASIAFVFSDVLAKLATLDWPVAQLMAV
                     RGLFAVMISLAVVIVSGDRQALGGLTRPLMTARSATEAVLGITFLSALGMMPIADLTA
                     LLMLSPLVITAIATLLLGETVGWRRWSAILVGFAGMLLVVKPGGAQAQYPLYHVAAIL
                     GLAAVLGVALRDILTRKLGNALPTTVVTLGTSIGSWLAGLALSLFQPWAPFQWRPFLA
                     CLVAAVIVTVGNYFIALACRGADLSVVAPYRFSSVIWAIALGALVFGEVPGALSIAGI
                     ALIAASALYTLHRERVRARTRLDGGQR"
     gene            3703..4329
                     /locus_tag="PLQ11_06125"
     CDS             3703..4329
                     /locus_tag="PLQ11_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011442270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HRK24512.1"
                     /translation="MAALLRLSRGIDWLNEKIGKNVAWLLLAAVLISTANAIIRKIFN
                     SSSNAWLEAQWLLFGAVFLLCAPWTLLHNEHIRIDIVNSKLPQRGKRLIEFLGHGLFL
                     IPMALVMIVTSYPFFKTSFLMNEQSSNAGGLPQWPAKFLILLGFILLLIQGISEFIKR
                     MAVSQGLIPDSLGSGGHHAAAEAEAERLLEVAREEAEKAERARLAKDQ"
     gene            4361..6373
                     /locus_tag="PLQ11_06130"
     CDS             4361..6373
                     /locus_tag="PLQ11_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRK24513.1"
                     /translation="MAAFIAHNIAPIMFAALVVFLLLGYPVAFALGACGLAFFVIGVE
                     LAPLSGGTITLSWPLLQSLPDRIYGGVMANDVLLAIPFFTFMGLVLERSGMAEDLLDT
                     IGQLFGTIRGGLAYAVIFVGALLAATTGVVAASVISMGLISLPIMLRYGYDRRLASGV
                     IAASGTLAQIIPPSLVLIVLADQLAADVGSMYEGAFVPGVVLSALYASYVFLVSTFYP
                     AAAPGLPPEDIAFKETDPAVANRLSRANAIALLAIIAAGVMFAGGSLKTQIVDGLGGG
                     WGAKIWAVILIGFLAWLALSVVRLVKTLQAGGDERGLLSLLTLTIVASVLAYLYMTTT
                     KVGAGADFVILTMSVGVAVAFLIAVANWICSRFLGFRFLSKLAQQVTFVMVPPLALIF
                     LVLGTIFIGVATPTEGGAMGATGALLLAAFKRYLDNDPKRFNWTIIRQATESTAKLSA
                     FVIFILIGARIFSLTFYGVNGHVWVEEHLKSLPGGFYGFLILINLAVFLLAFFLDFFE
                     LAFIVIPLIRPAAEAIFTTDPTALEIARSFNLTTDAEIKKAMIVWFGVMMGVNMQTSF
                     MHPPFGFALFYLRSVAPKEPYKDRVTGKMTDPVTTGQIYWGAVPFVVIQLIMVTLVML
                     TPSMVITKKPERPAVDPSKALEQLNNLGGGIGAPGGGGLDIAPPKF"
     gene            complement(6529..7617)
                     /locus_tag="PLQ11_06135"
     CDS             complement(6529..7617)
                     /locus_tag="PLQ11_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRK24514.1"
                     /translation="MKRRQFLTAGAGLAATAVAAPAIAQSMPEVKWRLTSSFPKSLDT
                     IYGASEVLAKYVAEATDNKFQIQVFAAGEIVPGLQAADAVTNATVELCHTAPYYYIGK
                     DPTFAFGTAVPFGLNARQQNAWMMHGGGLALMNEFYAKYNMIGFIGGNTGCQMGGWFR
                     KEIKTVADLSGLKMRIGGFAGQVLSKLGAVPQQIAGGDIYPALEKGTIDGAEWVGPYD
                     DQKLGFNKVAPFFYYPGWWEGGAMLKFLVNTAKWNELPKNYKAIFEAAAHLADNDMQA
                     KYDARNPTALIDLVRNGGAQLRAYPQEVMEACLKAANEVYTETNAKNAQFKKVHDSHM
                     AFRNDGYLWWQIAEYTFDNFMIRTRQRT"
     gene            complement(7822..8931)
                     /locus_tag="PLQ11_06140"
     CDS             complement(7822..8931)
                     /locus_tag="PLQ11_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018690253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HRK24515.1"
                     /translation="MSNADAVLAALAAVKGPDGRTSLPQSGALSQIAIAAGKAYFSIA
                     IDPKRAAAMEPMRKAAETAAAAVPGLSGAVVTLTAERAAASQAQAAAPPRPAPGGGKL
                     EVPGIKHIIAVASGKGGVGKSTTSANLALSLQRLGLKVGVLDADVYGPSMPKLFGLNT
                     RPVMAGNNMLQPLDGYGLKVMSIGFLVEEDSAMIWRGPMVMSAIQQMLREVAWGDLDV
                     LVVDMPPGTGDAQLTMAQNVPLAGAVIVSTPQDLALIDARRGITMFRKVDVPILGVIE
                     NMSTFICPQCGTRHDIFGHGGARAEAAKIGAPFLGEIPLAMAIRENSDAGRPIVLTSP
                     DGPHAAAYLSIARAVRDALSAGAGQRAMPAIVFDS"
     gene            9037..9975
                     /locus_tag="PLQ11_06145"
     CDS             9037..9975
                     /locus_tag="PLQ11_06145"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018428282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRK24516.1"
                     /translation="MSPGQPATAWRQQAAGSRTKELDMAKVAFLGLGVMGYPMAGHLK
                     AKGGHEVTVYNRTFAKAEKWAAQHGGTAARTPREAAAGKDFVFACVGNDDDLRAVTIG
                     PDGAFQAMARGAVFVDNTTASAEVARELATEAAKRGVGFVDAPVSGGQAGAENGLLTV
                     MCGGDEASYARAEPVIMAFARACRRLGPPGAGQLTKMVNQICIAGLVQGLAEGVHFAR
                     KAGLDVEAVLDVISKGAAGSWQMENRGKTMNQGKFDFGFAVDWMRKDLSICLTEARRN
                     GASLPVTALVDQFYAEVQKMGGNRWDTSSLMARLER"
     gene            complement(10865..11650)
                     /locus_tag="PLQ11_06150"
     CDS             complement(10865..11650)
                     /locus_tag="PLQ11_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007799952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRK24517.1"
                     /translation="MTYANLIVTIEHRVATLTLNRPAKLNAFSAGLHADLRSALAAAA
                     DDAGVRAVVLTGAGRAFSSGQDLTEDLPRHPDGRFDLSGPLMRDYNPLIERLMSYPKP
                     VIAALNGPAVGASMNIALACDIVLATRSAYLQEGFAKIALVPDAGGTWVLPRLVGFKR
                     ALALSLTADRVSADDAERMGLVYKVYDDAAFAGEVATLARRLADGPAHCFGLIKRALA
                     GSLDNDLGQQLAIEAEMQQAAADHPDFMEGVMAFLQKRPPQFK"
     gene            complement(11647..>12114)
                     /locus_tag="PLQ11_06155"
     CDS             complement(11647..>12114)
                     /locus_tag="PLQ11_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24518.1"
                     /translation="AALLACVAALPAMAADDPAQQAMPSPLQQVSGADLAQEVTGNTL
                     SGRHSSGMPYSEYHSPDGRIYGHNNNVPVRDGCWVIRRDEICYTYEKGPAPGVFCWRF
                     YRAAGGYTIYLPTTGTVGTAKLEAGNPYKYSDGGQPWSCDALLSMASPRAQNP"
BASE COUNT         2108 a         3858 c         3885 g         2263 t
ORIGIN      
        1 gatgcgctgc aggcgatctt ccttgctcat tacgaaaagc atatcgccga tttctcgcgc
       61 ccctaccccg gcctgatcgc ccagctcgac cggttgcagg cggacggcca tcgtctggcc
      121 gtctgcacca acaagctgga agcctatgcg cggctgctgc tggacgaact cgggctgacc
      181 caccgtttcg ccgccatcac cggcggcgac agcttcgcct tcaagaagcc cgatcggcgg
      241 catcttgaag agaccataag gctggccggc agcgggccgg cgatcatggt cggcgacagt
      301 cggactgacg ttgataccgc gcgcaatgcc ggcattccgg tgatcggggt cagcttcgga
      361 tataccgatg tgccgatgaa ggacctcagg cccgatgtgc tgatcgagca tttcgacgaa
      421 ctggccggcg cgatcgcccg gatcgactga agccggcctc ctcgacgggc cggccagcca
      481 ggcgcggcgg ccggccgttc ccggttctct gaaaaaccaa taaccgaagc aatccttcgg
      541 ctttcagtga cacaagaagg cttggcaatg gtgggcgcga cagggatcga acctgtgacc
      601 ccttcggtgt gaacgaagtg ctcttccgct gagctacgcg cccgatcagg accccgcgct
      661 tctagggagc cccgcgagcg aagtcaaggc tgcgccattg ttgcagacag gcaacggatc
      721 ggcgctactt cacgggaagt tgttccgatt cggcgctcat acgcttgaaa tagcgttcta
      781 ttgcggacaa aggtcgctac tcccatgctg ggtgtgcgcg caccctgatt cacgttactt
      841 gaaggatgca aggacatgcg tcgtttgaat acggccgttt tcgccctgct gttgggcgcc
      901 gtggcggcgc cggcagcagt cgcctatgag atcgacccgc tgacccgcga accgatcatc
      961 ccctatcagg acacccgcgc caaggcgacg ccgatcccgc gcgaggaagt cagctatgcg
     1021 tcgaagttcg cgccgggcac catcgtggtg agcacggtcg agcgccggct ctactacatc
     1081 ctcggcaacg ggcgtgccgt gcgctacggc gtcggcgtcg gccgtccggg ctttgaatgg
     1141 ggcggcagca aggcgatcac ccgcaaggcc gagtggccgg actggacgcc gccgccgcag
     1201 atgctcaagc gccgccccga cctgccacgc cacatgaagg gcgggccgga gaatccgctc
     1261 ggcgcccgcg ccctctatct gggctcgtcg ctctatcgca tccatggctc gaacgagccg
     1321 gagacgatcg gccaggcggt ttcctcgggc tgcatccgca tgctcaatga cgatgtggtg
     1381 gacctttaca gccgcgtgaa ggttggcacc cgggtcgttg tcctgcgctg agcgggacga
     1441 catcgaaggt ttcagcaggg gccgggcaac cggccctttt tgctgcgcgc gacccaagcc
     1501 gccttgacgg atgcggcgcc ctgccccaac tgtgaggcag taatgtgaat tcatcgccag
     1561 aaaagaaaga ccgcccctcg tgactgaccg agcgttgccg accggaatgt cccccgcgcc
     1621 gctcaccgac ccgttcgggc ggcaggtctc ctatctgcgc gtttcggtga cggaccgctg
     1681 tgacttccgc tgcacctatt gcatgtcgga gcacatgacc ttcctgccca agaaggatct
     1741 gctcagcctc gaggaactgt cgcggctgtg cggattgttc gtccagcgcg gtgtccgcaa
     1801 gctccgcatc accggcggcg aaccgctggt gcggcgggac atcatgcggc tgttccgcga
     1861 tctgtcgcag catctggcaa acggcgcgct ggacgaactt acgctgacta ccaacggctc
     1921 gcagctcgcc cgttttgccg atgaactcgc cgattgcggc gtcaagcgga tcaatgtctc
     1981 gctcgatacg cttgatgcgg acaaattccg ctccatcacg cgctggggcg atcttgatca
     2041 ggttctcgac gggatccgcg ctgcgcaagc agccgggctg aagatcaaga tcaacaccgt
     2101 tgccctgcgc gatttcaacg atcatgaaag cctggaactg atccgctggg cgcatggcca
     2161 gggcatggac atcaccttca tcgaagtgat gccgctcggt gagatggagg tggcgcgtgt
     2221 cgatcagtac ctgccgctgt cgcagctcag ggaaaggctt ggccagcagc tgacgctgaa
     2281 ggcgatcgac tatcgcaccg gcggtcccgc acgctacatg cgggccagcg agaccggcgg
     2341 ccgcatcggc ttcatcacac cgatgacgca caacttctgc gaaagctgca accgcgtccg
     2401 gctgacctgc accggaacgc tctacatgtg ccttggccag gaggatgcgg cggacctgcg
     2461 ccaaccgctg cgctcgtcgg aaggagacga ggcggtgctg cgtgccattg acgatgccat
     2521 cgcccgcaag ccgcgcggcc atgatttcat cattgaccgc gatacgcgcc agccggccgt
     2581 gcggcgacac atgagcctga cgggtggctg acaagccgcc ggcaacaggg ttctttcgtg
     2641 ttctccaacc tttccccgaa tggccgcggc atcctggcga tgaccggcgc gtcgatcgcc
     2701 ttcgtgttca gcgatgttct ggcaaagctt gcaacactgg actggccggt ggcgcagctg
     2761 atggcggttc gcggcctgtt cgcggtgatg atcagtcttg cagtggtgat tgtcagcggc
     2821 gaccggcagg ccctgggagg gctgaccaga ccgctgatga ccgcgcgttc ggcgacggaa
     2881 gcggttctgg ggatcacctt cctgagcgcg ctcggaatga tgccgattgc cgatctgacc
     2941 gcattgctga tgctcagccc gctggtcatt acggcgatcg ccacattgtt actgggagaa
     3001 accgtcggat ggcggcgctg gagcgccatt ctcgtcggct ttgccggcat gctgctggtg
     3061 gtcaagcccg gcggagccca ggcccaatat ccgctctatc acgtcgcggc gatactcggg
     3121 cttgccgcag tgcttggcgt tgccttgcgc gatatcctga cacgcaagct cggcaatgcc
     3181 ctgccgacca cggtggtcac gctgggaaca tccatcggtt cctggctagc gggcctcgcc
     3241 ctcagcctgt ttcaaccctg ggcaccgttc cagtggcggc cgttcctggc ctgccttgtc
     3301 gcagcggtga ttgtcacggt cggcaattac tttatcgcac tggcctgccg cggcgccgat
     3361 ctctcggtgg tggcacccta ccgcttttca tcggtcatct gggccatcgc gctgggagcg
     3421 ctggtgttcg gcgaagttcc gggcgccctg tccatcgccg gcattgcgct gattgccgca
     3481 agcgctctct acactctgca tcgcgaacgg gtgcgcgccc gcacgcgact tgacggtggg
     3541 caacgctgac aggcaaggca tgcaagaatc gcatatgcct cctgcctgcc agtcagcttg
     3601 ccagatgctt gaccaatgtc taaagacttg ggttaatcga tcgttcatgt tccgggaggg
     3661 gcaacggtcg ctgcgggaga atgcacaaac cgggagggac gcatggcggc cttgttgcgc
     3721 ctgtcgaggg gtattgactg gctgaacgaa aagatcggca agaacgtggc ctggctactg
     3781 ctggcagcgg tgctgatttc aaccgccaat gcaatcatcc gcaagatctt caattccagt
     3841 tcgaatgcct ggctcgaagc gcaatggctg ctgttcggcg cggtgttcct gctgtgcgct
     3901 ccgtggacgc tgctgcacaa cgagcacatc cgcatcgaca tcgtgaactc caagttgccg
     3961 cagcgcggca agcgcctgat cgagtttctc ggccatggcc tgttcctgat cccgatggcg
     4021 ctggtgatga tcgtcacctc ctatcccttc ttcaagacct cgttcctgat gaacgagcaa
     4081 tcgagcaacg ccggcggcct gccgcagtgg ccggcgaagt tcctgatcct gctcgggttc
     4141 atcctgttgc tgatccaggg catttcggaa ttcatcaagc ggatggcggt gtcgcagggt
     4201 ctgatcccgg actcgctcgg ctcgggcggt catcatgctg cggcggaagc cgaggcggag
     4261 cgcctgctgg aggttgcgcg cgaggaggcc gaaaaggccg agcgcgcccg gcttgccaaa
     4321 gaccagtaat cagtcccgat cccgcgcgga gtccttcgct atggccgctt tcatcgccca
     4381 taatatcgcc ccgatcatgt tcgcggcgct ggttgtcttc ctgctgcttg gctatccggt
     4441 cgctttcgcg ctgggtgcct gcggcctggc cttctttgtc atcggcgtgg aacttgcgcc
     4501 gctgtcgggc gggaccatca ccctgtcctg gcccttgctg caatcgctgc cggaccgcat
     4561 ctacggcggc gtgatggcca acgacgtctt gctggccatt ccattcttca ccttcatggg
     4621 gctggtgctg gaacggtccg gcatggcgga ggacctgctc gacaccatcg ggcagttgtt
     4681 cggcacgatc cgcggtggtc ttgcctatgc ggtcatcttc gtcggcgcgc tgctggcggc
     4741 gaccaccggc gttgtggcgg cctcggtgat ctcgatgggg ctgatctcgc tgccgatcat
     4801 gctgcgctat ggttatgacc gacgcctggc ctccggcgtc atcgcggcgt cgggcacgct
     4861 ggcgcagatc attccgccgt cgctggtgct gatcgtgctg gccgaccagc tcgccgctga
     4921 cgtcggctcg atgtatgaag gcgccttcgt tccaggcgtg gtgctgtcgg cgctttatgc
     4981 ctcttatgtg ttcctcgtca gcacgttcta tccggccgct gcgccgggcc tgccgccgga
     5041 ggatatcgcc ttcaaggaaa ccgatcctgc ggttgccaac cggctgtcgc gcgccaatgc
     5101 gatcgctttg ctggcgatca tcgcggccgg ggtgatgttc gccggcggtt cactcaagac
     5161 ccagatcgtc gatggtctgg gcggcggctg gggcgccaag atctgggcgg tgatcctgat
     5221 cggcttcctc gcctggcttg ctctttcggt tgtccggctg gtcaagacgc tgcaggccgg
     5281 cggcgatgag cgcggcttgc tgtccttgct gacgctgacc atcgtcgcgt cggtgctcgc
     5341 ctatctctac atgacgacga ccaaggtcgg cgcaggcgcg gatttcgtca tcctgacgat
     5401 gtcggtcggc gtggcggtcg ccttcctgat tgcggtcgcc aactggatct gctcgcggtt
     5461 cctgggcttc cgcttcctgt ccaagctggc ccagcaggtg accttcgtca tggtgccgcc
     5521 gctggccctg atcttcctgg ttctcggcac catcttcatc ggcgtcgcca cgccgaccga
     5581 aggcggcgcg atgggcgcca ccggcgcgct gctgctcgcc gcgttcaagc gctacctcga
     5641 caatgatccg aagcgcttca actggacgat catccgccag gcgacggaat cgaccgccaa
     5701 gctgtcggcc ttcgtgatct tcatcctgat cggcgcgcgc atcttctcgc tcaccttcta
     5761 cggcgtgaac ggccatgtgt gggtggaaga gcacctgaag tcactgccgg gcgggttcta
     5821 cggcttcctg atcctcatca acctcgccgt gttcctgctc gcgttcttcc tcgacttctt
     5881 cgaactggcg ttcattgtca tcccgctgat ccgtccggca gcggaagcaa tcttcaccac
     5941 cgatccgacg gcgctggaga ttgccagaag cttcaacctg acgacggacg cggaaatcaa
     6001 gaaagcgatg atcgtctggt tcggcgtgat gatgggcgtc aacatgcaga ccagcttcat
     6061 gcatccgccg ttcggttttg cgctgttcta cctgcgctcg gtggcgccga aggagccgta
     6121 taaggatcgc gtgaccggca agatgaccga tccggtcacc accggccaga tctactgggg
     6181 cgccgtgccg ttcgtggtca tccagctgat catggtgacg ctggtgatgc tgacgccgtc
     6241 gatggtcatc accaagaagc cggaacggcc ggcggtcgat ccaagcaagg cgctcgaaca
     6301 gctcaacaac ctcggtggcg gcatcggcgc cccgggcggc ggcgggctgg atatcgcacc
     6361 gccgaagttc tgacggccca agacctggct tgacgatcat gcagcggcgc ttcggcgccg
     6421 ctgttttttt gtgccgtcag ggcagtccca aaaaacaagc ggcgccagaa agccccaaaa
     6481 gaaaacggcg cccgaaggcg ccgttccatt tgatctcgat cacgccgctc aggtgcgctg
     6541 gcgggtgcgg atcatgaagt tgtcgaaggt gtattcggca atctgccacc acagatagcc
     6601 gtcattgcgg aaggccatgt gcgagtcatg caccttcttg aactgggcgt tcttggcgtt
     6661 ggtctcggta tagacctcgt tggcggcctt gaggcaggct tccatgacct cctgcggata
     6721 ggcacggagc tgggcgccgc cgttacgcac cagatcgatc agcgcggtcg ggttgcgggc
     6781 gtcatacttg gcctgcatgt cgttgtcggc gagatgcgcc gctgcctcga agatcgcctt
     6841 gtagttcttc ggcagttcat tccacttggc ggtgttgacg aggaacttca gcatggcgcc
     6901 gccttcccac caacccggat agtagaagaa cggggcgacc ttgttgaagc cgagcttctg
     6961 gtcatcatag gggccgaccc attcggcgcc gtcgatggtg cccttctcca gcgccggata
     7021 gatgtcgccg ccggcgatct gctgcggcac agcgccgagc ttggacagca cctgaccggc
     7081 gaagccgccg atacgcatct tgaggccgga gagatcggca acggtcttga tttccttgcg
     7141 gaaccagccg cccatctggc agccggtgtt gccgccgatg aaaccgatca tgttatactt
     7201 ggcgtagaac tcgttcatca gcgccagacc gccgccgtgc atcatccagg cgttctgctg
     7261 acgggcgttg aggccgaacg gaaccgccgt gccgaatgcg aaggtcgggt ccttgccgat
     7321 gtagtaatag ggcgcggtgt ggcagagttc cacggtggcg ttggtcaccg cgtcggccgc
     7381 ctgcaggccg ggaacgattt cgcccgccgc gaaaacctgg atctggaact tgttgtccgt
     7441 cgcctcggcg acgtatttgg ccagcacttc tgatgcgcca tagatggtat cgagcgactt
     7501 cggaaagctc gacgtcaggc gccacttcac ttcaggcatt gactgggcaa tggccggtgc
     7561 ggcaactgcg gtagccgcaa ggcctgcgcc tgcggtgaga aactggcgac gcttcatgct
     7621 tatcctcccg gatggtcagt ggaatatgcc aaaacccgcg ccgaacgggt cggcgcgcgt
     7681 tggggcaata caaagcagaa acatggcccg ctgcaaacag gaatctgctc tatcctgccg
     7741 acaaagtgaa cgacagaatc cggcgtctat ccgtggaaaa ccagcccagg agccgcgcct
     7801 taccggccga aaagcgccgc cctagctatc gaacacgatg gccggcatgg cgcgctggcc
     7861 agcccccgcc gacagggcat cgcggaccgc gcgcgcaatc gacagatagg cggctgcgtg
     7921 cggtccgtcc gggctggtga ggacaatcgg ccgacccgca tcggaattct cgcgaatggc
     7981 catggccagc gggatttcac ccaggaacgg cgcgccaatc ttggccgcct cggcccgcgc
     8041 gccaccatgt ccgaagatgt cgtggcgcgt gccgcactgc gggcagatga acgtcgacat
     8101 attctcgata acgccgagga tcggcacatc gaccttgcgg aacatggtga tgccgcgccg
     8161 ggcgtcgatc aacgccagat cctgcggggt cgacacgatc accgcgccgg ccagaggcac
     8221 gttctgcgcc atggtcagtt gcgcatcgcc ggtgccgggc ggcatgtcga ctaccagcac
     8281 atcaagatcg ccccaggcaa cctcgcgcag catctgctgg atggcgctca tcaccatcgg
     8341 cccgcgccag atcatcgcgc tatcttcctc gacgaggaag ccgatcgaca tcaccttcag
     8401 cccgtagcca tcgagcggct ggagcatatt gttgccggcc atgaccggac gggtgttgag
     8461 gccgaacagc ttgggcatcg acgggccata gacatccgcg tcgagcacac cgaccttcag
     8521 cccgagccgc tgcaatgaca gcgccagatt ggccgatgtc gtcgacttgc cgaccccgcc
     8581 cttgccactg gcgaccgcaa tgatgtgctt gatacctgga acctccagct tgccgccacc
     8641 cggggcaggc cgaggcggcg cagcagcctg ggcctgggag gccgcggccc gttcggccgt
     8701 cagggtcacc accgccccgg aaagacccgg aacggctgcg gctgctgttt cggccgcctt
     8761 gcgcatcggc tccatcgccg ctgcccgctt tgggtcgatg gcaatcgaaa aatatgcctt
     8821 gccggctgcg atggcgatct gcgacagcgc cccagactgc ggcagggatg tccgcccgtc
     8881 cggccccttg acggcggcga gggcagcgag aacggcatcg gcattgctca tcggtcagtc
     8941 tctccaggca aaggacaatc gtccgccgcc atttgcccgg atggcagcgc tccggtcaac
     9001 tgcttcatca gcggccggtg aacttcccct ccctttatct ctcctggcca accggctaca
     9061 gcttggcggc aacaagcggc gggcagccgc accaaggagc tggacatggc gaaagtggcg
     9121 tttctcggcc tcggcgtgat gggatatccg atggccggac atctcaaggc caagggcggg
     9181 catgaggtga ccgtctataa ccggacgttt gcgaaggcgg agaaatgggc cgcccagcat
     9241 ggcgggacgg ccgcccgcac gccaagagaa gccgcagcgg gcaaggattt cgttttcgcc
     9301 tgtgtcggca acgacgacga tctgcgcgcg gtcaccatcg gtcctgacgg agccttccag
     9361 gccatggcca gaggcgcggt gtttgtcgac aacaccaccg ccagcgcgga agtcgcccgg
     9421 gaactcgcca ccgaggcggc aaagcgcggg gtcggttttg tcgatgcacc ggtctccggc
     9481 ggccaggccg gcgccgaaaa cggcctgctg accgtcatgt gcggcggcga tgaggcaagc
     9541 tatgccaggg ccgaaccggt gatcatggcc tttgcccgcg cctgccgccg gctcggccct
     9601 cccggagcgg gacagttgac caagatggtc aaccagatct gcatcgccgg cctggtgcag
     9661 gggctcgccg agggtgtgca tttcgccagg aaggccgggc tcgatgtcga ggcggtgctg
     9721 gatgtcattt ccaagggcgc agctggatcc tggcagatgg aaaaccgcgg caagacaatg
     9781 aaccagggca agttcgactt cggcttcgcc gtcgactgga tgcgcaagga cctgtcgatc
     9841 tgcctgacgg aagcacggcg caacggcgcc agtctgccgg tgaccgcgct ggtcgaccag
     9901 ttctatgccg aagtgcagaa gatgggcggc aaccgctggg atacgtccag cctgatggcc
     9961 cgcctcgaac gctgagtggt gatgccggcc ctggccggag gcaagggcgc tcctgactgt
    10021 caatccctcc cccttgcggg gagggtggtc cgagcatcgc agacaagttt acgcagtctg
    10081 cgtagacttg actgcgagcg gcggaggacc gggtgggggt ggttcaaccc ctcacctcac
    10141 cccaccccgg cgctgacgcg ccgaccctcc ccatcaaggg gagggaagaa cgggtgtggc
    10201 gcccctcgca tcggctcggc gtcgcgttgc tcctcgattc tttttgtgct gtcaggccag
    10261 acagccctga gtgatactct gaacaataaa cggcgcaacg gggaggccgt tgcgccgaat
    10321 tcctggcggt gatccgccga tgcgtcaggc cgctctgccg tcaaccgggg cggtcggggg
    10381 cgcggggcag accgaagaga ctggccacaa cctcgcctga acagccctgt ttgaggcgct
    10441 cgccatggct gggaagctga gcacatccgc tcacggcggc ggcagggcaa tcatggaggt
    10501 ttggggaagc tggcaagcga ggcaaacgca tgggcctgtc tcctgtcaag gaagcgcccg
    10561 ggggttccgc ctgtcgcgtc atccaatccg gccgcaacaa ggccagcgtg ccgcaggatc
    10621 gtttgcaaaa ggttaacggc gcttgccctg ctcacccggt ggtgaacaaa gccgaagccg
    10681 gcagatcggc gaacagccgg ttgcattcag ggatctgcaa gcatgccaaa ccatgccctc
    10741 gtattgattg aacatcaaag cgagagataa catgatcgtt atcaagagtt tagagcatgc
    10801 tttttgcgat tagccggatt ccacccaatc gcagcatgct ctaaggcgcc cgacccgttc
    10861 gggctcactt gaactgcggc gggcgcttct gcaggaaggc catcacccct tccatgaaat
    10921 cgggatggtc cgcagccgcc tgctgcatct cggcctcgat ggcgagctgc tggccaaggt
    10981 cattgtcgag gctgccggcc agcgcccgct tgatcaggcc gaagcaatgg gccggaccat
    11041 cggcgagccg gcgggcgagc gtggccactt ccccggcgaa agccgcatcg tcatagacct
    11101 tgtagaccag ccccatccgc tcggcatcgt cggcgctgac ccgatcggcg gtcagcgaca
    11161 gggcaagcgc gcgcttgaac ccgaccagac gcggcaggac ccaggtgccg ccggcatccg
    11221 gcaccaaagc gatcttggca aacccttcct gcagataggc cgaacgggtg gccagcacga
    11281 tgtcgcaggc aagcgcgatg ttcatcgacg cgccgaccgc cgggccattg agcgccgcga
    11341 tcaccggctt gggatagctc atcaggcgct cgatcagagg attgtagtcg cgcatcagag
    11401 ggccggagag gtcaaagcgc ccatcaggat ggcgcggcag gtcctcggtc aggtcctgcc
    11461 ccgaggagaa ggcgcggccg gcgccggtga gcaccacggc gcgcacgccc gcatcatcgg
    11521 cagccgctgc cagcgcagaa cggaggtcgg catgcaaccc ggcagagaag gcattgagct
    11581 tggccgggcg gttgagcgtc agggtggcga cgcgatgctc aatggtgacg atcagattgg
    11641 cgtaggtcat gggttctgtg ctcgtggtga tgccatggag agcagcgcat cgcacgacca
    11701 gggctggccg ccgtcgctgt atttgtaagg gtttccggct tccagcttgg ccgtgccaac
    11761 cgtgccggtc gtgggcagat agatcgtgta gccgccggcg gcgcgataga agcgccagca
    11821 gaacacgccc ggtgccggcc ccttctcata agtgtagcaa atctcgtccc gccggatgac
    11881 ccagcagcca tcgcgcaccg gcacattgtt gttgtgacca tagatgcggc catcgggtga
    11941 atgatactcg ctgtagggca tgcccgacga atgccgcccg gacagggtat tgcctgtcac
    12001 ctcctgcgcc agatcggcgc ccgagacctg ctggagcggc gagggcatgg cctgctgcgc
    12061 cgggtcatcg gcggccatgg ccggcagcgc cgccacgcag gccaggagtg ccgc
//
LOCUS       DAOMXG010000066        12046 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_2869_length_12046_cov_10.955300, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000066 DAOMXG010000000
VERSION     DAOMXG010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 12046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12046
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2869_length_12046_cov_10.955300"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(203..1594)
                     /gene="argH"
                     /locus_tag="PLQ11_06160"
     CDS             complement(203..1594)
                     /gene="argH"
                     /locus_tag="PLQ11_06160"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HRK24519.1"
                     /translation="MANAMWGGRYQEGPAAIMEEINASIDFDKRLASQDIRGSKAHVA
                     MLARQGIVSAADADAITEGLEAIEAEIVAGTFTFSRALEDIHLNIEKRLADRIGPAAG
                     RLHTARSRNDQVAVDFRLWVRDQVDAVDAGLKDLQQALADRALEHAGTIMPGFTHLQS
                     AQPTTFGHHLLAYVEMLGRDRGRFRDARIRMNECPLGAAALCGTSFPIDRHATAAALG
                     FDRPTANSLDSVSDRDFALESLAAMAISAMHLSRLAEELVIWTTPQFGFVALSDKFTT
                     GSSIMPQKRNPDAAELVRAKIGRIIGALTGLLVVMKGLPLSYAKDMQEDKERTFDAFD
                     AFALCVAATAGMVRDLVPSARAMRKAAGSGYATATDLADWLVRTLNMPFRDAHHVTGR
                     IVGAAAGRGCELERLPLAEMQAIEPRITQDVYSVLGVANSVRSRTSYGGTAPANVRRQ
                     ARRWLTRLKRDGG"
     gene            1608..2273
                     /locus_tag="PLQ11_06165"
     CDS             1608..2273
                     /locus_tag="PLQ11_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRK24520.1"
                     /translation="MHTHPDPAPPATTRSGLLIAGGLLVAVVAGIAGLYGMRGDGANS
                     HIGGDCAPARGVAARIAPLATGEVAALKVTPQPRPAVDLGFLRPDGTPARLADWRGKT
                     VLVNLWATWCAPCRAEMPALDRLQKALGGADFEVVTINIDTNRLDRPKAFLNEVGVAA
                     LAYYSDPTAAVFADLKKAGKAFGMPTTLLIDQNGCELGAMAGPAEWSSPDALALLRAT
                     LGR"
     gene            complement(2400..3695)
                     /locus_tag="PLQ11_06170"
     CDS             complement(2400..3695)
                     /locus_tag="PLQ11_06170"
                     /inference="COORDINATES: protein motif:HMM:NF014577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HRK24521.1"
                     /translation="MQRKHLLARSAALAFGIAVASPACVHAADLKAPPLQPGAVAEPM
                     RACPEYGPGFIRLPNTDICLKAAIDIAYEIGLDLARRDLNIETTTVGGHPVALYEVQR
                     LGREIDRFATRLDGRMNVTTVSKIGDEPVITFLSFRSSPNVTAATVRDHVGLTSSVHV
                     HEAWVKYAGFTAGRHPSYFDFVPGYNHTAGYASQRNLSLLAFTKSFDKAGSVSVAVED
                     QTERRMEEGVWAAYGGQRMPDVVAQARWTPSWGIVHLGGALHHINDAIGSRSAYGFAA
                     NAGVEYRVKWGEVFGPAAEGTYGRILVTGAYASGALDYLGIPKFGADYVSDITGRIEQ
                     TKGHSAIVSYEHVWRPNFKTTFGVSSYGLGSDTENYRHRVRGLLAQVGAEYMPTPGLM
                     VGVEGDYFRDSVKGRFFGVQGPRDKVDIFTAFAYVRRRI"
     gene            4077..4421
                     /locus_tag="PLQ11_06175"
     CDS             4077..4421
                     /locus_tag="PLQ11_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24522.1"
                     /translation="MRIASAAILATALFAGPALAGDISVYQEGRSNWIAANQYNGANS
                     AWIQQHGINANGNNYAAFAQTGRSNFLNSVQTGYTDNILSANQVGRFNGAEAYQRALG
                     VNTFSVTQSRRR"
     gene            4647..5099
                     /locus_tag="PLQ11_06180"
     CDS             4647..5099
                     /locus_tag="PLQ11_06180"
                     /inference="COORDINATES: protein motif:HMM:NF018688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24523.1"
                     /translation="MRLLSIATGIAALVLGATAVQAQSATYSQSNTLNVAEIDQGAAS
                     NALQMQQSGVKNYLFARQVGSTNKARVDQKGTYNGAFLAQYGDDNSATFQQVDGASYV
                     TAASGGRIARSVETSGGVTTYVTTYTNGPISILQAATQPQAFGRLGRR"
     gene            5257..5742
                     /locus_tag="PLQ11_06185"
     CDS             5257..5742
                     /locus_tag="PLQ11_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24524.1"
                     /translation="MTTLKFSIAAGALALAGGLFAAQPAAALTMQECSAKYNAAKAAN
                     SLGGLSWNDFRKAQCGDDAPAAAPAPAPAARSPQQNAPAAAPRTQTALPASNAVFPRA
                     IDRKYATETPAKQRMKTCLDQYNANKAAGANGNGGLNWIQKGGGYYSQCNARMKQIQG
                     V"
     gene            complement(5798..6382)
                     /locus_tag="PLQ11_06190"
     CDS             complement(5798..6382)
                     /locus_tag="PLQ11_06190"
                     /EC_number="2.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009211461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-S-isoprenylcysteine O-methyltransferase"
                     /protein_id="HRK24525.1"
                     /translation="MTPTIAKAAYVLMAALWYVLRIPHERKARKTPIARDALDARERL
                     RLTVSLTGLGIVPMIYVATGWPAFAARSFSPLAAWLGVVLAVAALVVFRLTHKALGRY
                     WSVSLQMRKEHKLITEGIYSRIRHPMYSAFWLWALAQALLLPNWIAGLSGIIGFGTLY
                     LLRVGPEERMLLDTFGDEYRAYMARTGRLLPKLF"
     gene            complement(6379..7584)
                     /locus_tag="PLQ11_06195"
     CDS             complement(6379..7584)
                     /locus_tag="PLQ11_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24526.1"
                     /translation="MTGSVKQRTVAYLGGYYPLAPDLAHERFVRDLATVSDTWSLNAK
                     AGPATEHEHASRWPVTLAGPDWRADTDVYLFCWNDLIDADRSRSWFLRLPLALWVYAD
                     FVLHGAFWRYLTRAWRYCLFFLYPFVLLGVLAGLAWLIAAALPLAILGPGWAMVLRLV
                     GALAAFAVLARVIGRPLMLDHLLDDWIYARRLLTHGDAEVDRRLDRLADDLASRGGEL
                     LVVGHSLGAVHGAELIAKLLARRPEGPPVHFVTLGSSILKLGYHSGSFRLRATLAEIA
                     ASPRLVWQDFHAHNDPMNFYRREPVSELGLTARPAGMRSVRFRTMLSEARYRRIRRNF
                     FRLHSQFVSANDRRARYDYYLMLCGPFALPVWAGSDAGFSGLIGEDGRLTDAGKAALE
                     PAAVDGSRR"
     gene            complement(7800..9095)
                     /locus_tag="PLQ11_06200"
     CDS             complement(7800..9095)
                     /locus_tag="PLQ11_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRK24527.1"
                     /translation="MFSLEIFVLLALTLLNGFFAMSEMAIISARRARLDAMAEAGDHG
                     AAKAADLAREPTGFLSSVQIGLTLISIGTGAFGTATLSNDLVAPISAFEILRPHAEAI
                     AIGVIVVITTLLTLVFGELVPKRIALAYPETIAARVARPINLLAVGARPVVALLGVST
                     AFILRLLRVPTVRDQSVTEEEVQTILQEGTESGVIDPQERVMVKEVIGLAERSIAAIM
                     TRRSEVYWIDVEDDPKVVRAELRQCPFSRVVVAKDGSINEPLGYVHKKDLLDVLLEGK
                     PLDIEKNVREPLFVPEAASVLDVLELFRTKRNHFAFVIDEFGAFEGVVTLTDVLEAIA
                     GDIPEEHEVTRQAIVTRADGTYLVDGRAEIAELEETVGIAVPEDARFHTVAGLALELF
                     GRIPVEGDTADMEDGWTIEVIDMDGRRIDKLLIRRAANA"
     gene            complement(9226..9489)
                     /locus_tag="PLQ11_06205"
     CDS             complement(9226..9489)
                     /locus_tag="PLQ11_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HRK24528.1"
                     /translation="MTFKTRIATKLATLSPLHLEVIDESHRHHGHAGWKEGGETHFRV
                     IVTASAFAGQSRLARHRIINALLADELKERVHALAIEARAPGE"
     gene            9588..10178
                     /locus_tag="PLQ11_06210"
     CDS             9588..10178
                     /locus_tag="PLQ11_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015823431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HRK24529.1"
                     /translation="MDFNSSLFDKIRIARAREEQQPAERRCEHPGCQRPGNHRAPMGR
                     HREGRYFHFCLDHVRAYNQSYNYFAGMNDESIQAFQKDAITGHRPTWSMGIKGSRGPD
                     LEADDTISDILGLFRNGRRTTERREERRAVSAGASKALDALGLEEGAGRAEVRAQFKS
                     LAKRFHPDLNGGDRSHEEKLRAIIDAYNYLKSAGLA"
     gene            10245..11231
                     /gene="cobS"
                     /locus_tag="PLQ11_06215"
     CDS             10245..11231
                     /gene="cobS"
                     /locus_tag="PLQ11_06215"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008192290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobS"
                     /protein_id="HRK24530.1"
                     /translation="MSEAVTAGSPMPDTEFSVRALFNIDSDIKVPAYSKASDHVPELD
                     PDYLFDRDTTLAILAGFARNRRVIVTGYHGTGKSTHIEQVAARLNWPCVRVNLDSHVS
                     RIDLVGKDAIVLKDGKQVTEFRDGILPWAYQHNVALVFDEYDAGRPDVMFVIQRVLES
                     SGRLTLLDQSRVIRPHGAFRLFATANTVGLGDTSGLYHGTQQINQAQMDRWNIVTVLN
                     YLPHDQEVGIVLAKARHYRNKEGKDIVSKMVRVADLTRNAFMNGELSTVMSPRTVITW
                     AENADIFKDVAFAFRVTFLNKCDELERGTVAEFYQRCFGVELKESTVNVVLT"
     gene            11246..11941
                     /locus_tag="PLQ11_06220"
     CDS             11246..11941
                     /locus_tag="PLQ11_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020090718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HRK24531.1"
                     /translation="MTRPVALFFDIFGTVVDWRGSIAREARERFGETFAGEAFADAWR
                     DEYQPAMERVRTGGRGFVKLDVLHRENLDRILPRFGLDRLTEAERADLNLVWHRLDGW
                     PDASLALRRLGVLYRLAPMSNGNISLMVDLARHNHFHWDAILGAELARDYKPKLAVYR
                     SGCDALDLAPERCMMVAAHTSDLVAAKAAGLQTAHVARPDEYGPGKGEAAPSAPVDLA
                     AHDLDHLAELLEC"
BASE COUNT         2393 a         3842 c         3654 g         2157 t
ORIGIN      
        1 ggccctcgtc ggaatccggc ggcggctcca gcgggccgcg cttgccgcag gccgccacgg
       61 cgagaaccgt gccgatgccg atggcgcgca agagatggcg gcggctgggg gcggcggaca
      121 aggagatatt ggtcatgctc atatcaggac ttggaacatt tgaggccaga taacatgctt
      181 tgcgggttcg gcgcgaccaa aatcagccgc catcccgctt gagccgggtc agccagcgcc
      241 gtgcctgccg gcgcacgttg gccggggcag tgccgccata gctggtgcgg ctccggaccg
      301 aattggcaac cccgagcacg ctgtagacgt cttgtgtgat gcgcggctcg atcgcctgca
      361 tctcggcgag cggcaggcgc tcaagctcgc agccgcgccc cgcagctgcg ccgacgatgc
      421 ggccggtgac gtgatgggca tcgcggaacg gcatgttgag cgtgcgcacc agccagtcgg
      481 caaggtccgt ggcggtggca tagccggagc ctgcggcctt gcgcatggcc ctggcgctgg
      541 ggacaagatc ccgcaccatc ccggctgtgg cggcaacgca gagtgcgaag gcgtcgaaag
      601 cgtcgaacgt gcgctccttg tcctcctgca tgtccttggc atagctgagc ggcaacccct
      661 tcatgaccac cagcaggccg gtcagcgcgc cgatgatacg gccgatcttg gcgcgcacca
      721 gttccgccgc atcgggattg cgcttctgcg gcatgatcga ggaaccggtg gtgaacttgt
      781 ccgaaagcgc gacgaagccg aattgcggcg tcgtccagat gaccagttcc tcggccagcc
      841 gcgacagatg catggccgaa atcgccatgg cggccagcga ctcaagtgca aagtcgcgat
      901 ccgacacaga atccagcgaa ttggcggtcg gccggtcgaa gccaagggcc gctgcggtgg
      961 catggcgatc gatcggaaag gatgtgccgc acagggcggc cgccccaagt gggcattcgt
     1021 tcatgcggat gcgtgcatcg cggaatcggc cgcgatcgcg ccccagcatc tcgacatagg
     1081 cgagcaggtg gtggccgaat gtcgtcggct gggcactctg cagatgggtg aagcccggca
     1141 tgatcgtgcc ggcatgttca agcgccctgt ccgccagcgc ttgctggagg tccttcaggc
     1201 cggcatcgac cgcatcgacc tggtcgcgca cccacagacg gaaatcgacc gcgacctgat
     1261 cgttgcgcga acgggccgtg tgcagccggc ccgcagcagg gccgatccgg tcggctaggc
     1321 gtttttcgat gttgaggtga atatcctcga gagcccgcga aaaagtgaag gtaccggcga
     1381 caatttccgc ttcaattgcc tccagcccct cagtaatggc gtcagcatcc gcggcagaga
     1441 cgatcccctg tctggccagc atggccacat gagcttttga gccacggata tcctgagacg
     1501 ccagcctctt gtcaaaatcg atcgaggcgt tgatctcttc catgatcgcg gccggacctt
     1561 cctggtaacg gccaccccac attgcgttcg ccaaggtcat ctcctcgatg cacactcatc
     1621 ccgatcctgc cccacccgca acgacccgtt ccggcctgtt gatcgccggc gggcttctgg
     1681 tcgccgtcgt ggcagggatt gccgggctat acgggatgcg gggagacggc gcaaacagtc
     1741 atatcggcgg cgactgcgcg ccggcgcggg gggtcgcggc caggattgcg ccgctcgcaa
     1801 ccggcgaagt ggcggccctc aaggttacgc cacagccgcg ccctgcggtc gatctcggtt
     1861 ttttgaggcc cgacggcacg ccggcccgcc tcgccgactg gcgcggcaag accgtgctcg
     1921 tcaacctctg ggcgacctgg tgcgctccgt gccgggcgga aatgccggca ctcgaccggc
     1981 tgcagaaagc gcttggcggc gccgatttcg aggtcgtgac gatcaacatc gacaccaacc
     2041 gtctcgatcg ccccaaggcc ttcctcaacg aagtcggggt cgcagcgctc gcctattatt
     2101 ccgatccgac cgctgcggtg tttgccgatc tcaagaaggc cggaaaagcc ttcggcatgc
     2161 cgacaaccct gctgatcgat caaaacggct gtgagctcgg cgccatggca gggccggcgg
     2221 aatggtcgag ccccgatgct ctggccctgc tcagggccac gctgggtcgc tgaaaccgca
     2281 gccgctcata ccaagggtca tgataattga cccttgggtc ctgttttgaa ttctcatttc
     2341 ccattgaaaa atagtgaaat tcaaagttcg tcatcggtca cttcaagtga ccgatggtat
     2401 cagatgcggc ggcggacata ggcaaaggcg gtgaaaatat cgaccttgtc gcgcggcccc
     2461 tggactccga agaaccggcc cttcaccgaa tcgcggaaat aatccccctc gacaccgacc
     2521 atcaggccgg gggtcggcat atattccgcg ccgacctggg ccagcaggcc acgcaccctg
     2581 tgacgataat tctccgtgtc ggaccccagg ccatagctgg aaacaccgaa cgtcgtcttg
     2641 aagttcggcc gccagacgtg ttcgtacgag acgatggccg aatggccctt ggtttgctcg
     2701 atacgtccgg tgatgtctga aacatagtcg gcaccgaact tcggaatgcc gagataatcc
     2761 agcgccccgc tggcataggc tcccgtgacc aggatgcggc cataggtgcc ttcggcggcc
     2821 gggccaaaca cctcgcccca tttcacccgg tattccacgc cggcattggc cgcaaatccg
     2881 taggcgctgc gcgaaccgat cgcgtcgttg atgtgatgca gggcgccgcc gagatggacg
     2941 atgccccagg acggggtcca gcgcgcctgc gccaccacat cgggcatgcg ctggccaccg
     3001 taagcggccc agaccccctc ttccatgcgc cgttcggtct gatcctcgac cgcaaccgac
     3061 acgctgccgg ccttgtcgaa gctcttggta aaggcgagga ggctcagatt gcgctgcgat
     3121 gcatagccgg ccgtatggtt gtagcctggc acgaaatcga aataggacgg atggcgcccg
     3181 gcggtaaacc cggcgtattt cacccaggct tcatgcacat gaaccgaact ggtcaaacca
     3241 acatgatcgc gcaccgtcgc cgccgtgaca ttgggtgagg agcggaatga caggaaggtg
     3301 atgaccggct cgtcgccgat cttgctgacg gtggtgacgt tcatccgccc atccagccgc
     3361 gtcgcgaacc ggtcgatctc gcggccaaga cgctgcacct cgtaaagcgc gaccggatgg
     3421 ccgccgacgg tcgtggtctc gatattgagg tcccgcctcg ccagatcgag gccgatttcg
     3481 tacgcgatgt cgatcgccgc cttcaggcag atatcggtat tgggcaagcg gatgaagccg
     3541 ggcccatact ccggacaggc ccgcatcggc tcggcaacag cgcccggctg caggggaggc
     3601 gccttcagat cggcggcatg aacgcaagcc ggtgacgcaa cggcgatacc aaacgcaagg
     3661 gcggctgagc gggccaggag atgtttgcgc tgcactcgaa tcaactcgga tcgtggccgg
     3721 cactgggcgg cctgtttcac ttctggaaat attgcagcca acctgcctaa atattcatta
     3781 acgctaagag acactaaaca ctcaaaaact ggcccacaaa gccaacgaat gtcatgttta
     3841 atacacatta ataatcgttt ttaatcagtt tattcttggt attaagcaag atatttgatg
     3901 tgttttggca agttttacgg attaatagtg agatgaattg ctacatcact atcggtttaa
     3961 attaatgttt aataagtcat ttacttgaat attcggatca atttctagat atgccgacac
     4021 gagccaacga cagaattggc caaggctatc agacagagca aggacaggag tcattcatgc
     4081 gtattgcttc agcggccatt ctggccaccg cgttgttcgc gggccccgcc ctcgccggcg
     4141 acatttcggt ctatcaggaa ggccgctcca actggattgc ggccaatcag tacaacggcg
     4201 ccaattcggc ctggatccag cagcacggca tcaacgccaa tggcaacaac tatgcggcct
     4261 tcgcccagac cggccgctcc aactttctca attcggtcca gaccggctac actgacaaca
     4321 tcctgtcggc aaaccaggtt ggtcgcttca atggcgccga ggcctaccag cgcgcgctcg
     4381 gtgtcaacac cttctcggtg acgcagagcc gccgtcgcta ataccaaaag tcattgaaaa
     4441 tgactctttg gtgcaactca aaaatttctc atctcgttga taaaagtgag aaatttttga
     4501 aacgaaccaa gtcgcggcga gttccttgcc ttgcgacttg gtataaggcc ggctgcgctt
     4561 tttccaaacc catcctgccc tgatcgggcg cgcggcggca ggccgtcgcg cgtcggattg
     4621 gtgcatcctt atatccggag gccatcatgc gtctgctttc gattgcaacg gggattgcgg
     4681 ctctcgttct tggcgcaacg gccgttcagg cccaaagcgc gacatacagc cagtccaaca
     4741 ctctcaatgt tgccgagatc gaccagggtg cggcgtccaa tgccctgcag atgcagcaga
     4801 gcggcgtgaa gaactacctg ttcgcccgac aggtggggtc gaccaacaag gcccgcgtcg
     4861 accagaaagg cacctacaac ggcgcgtttc ttgcccagta tggcgacgac aacagtgcca
     4921 ccttccagca ggtcgatggc gccagctatg tcacggcggc aagtggcggg cggatcgccc
     4981 gcagcgtcga gacgtccggc ggtgtgacca cctatgtcac gacctatacc aacggtccga
     5041 tcagcatcct ccaggctgca acccagcccc aggctttcgg tcgccttggc cggcgctgat
     5101 ccgccgccaa ccccccttat gctcagcacc gcagaaaact gtgtgcatgc tgcaaggcat
     5161 ggtcattgcg ggttcattgt ctttaccata acttgcttgc cgaagtcgcg ccgggacagg
     5221 tgacggctcg gttggcctcg cgcttggaga cagacaatga ccacactgaa gttttcgatt
     5281 gccgctggcg cattggcgct tgccggcggc ctgttcgcgg cccagccggc tgcggcgctg
     5341 accatgcagg aatgcagcgc caagtataac gcggccaagg ccgccaacag cctcggcggc
     5401 ctgtcctgga acgatttccg caaggcccag tgtggtgatg acgccccggc ggccgcgcct
     5461 gctccggccc ccgcagcccg ctccccgcag cagaatgctc cggccgccgc tccgcgcacc
     5521 cagaccgcgc ttcccgcctc gaacgccgtg ttcccgcggg cgatcgaccg gaaatacgcc
     5581 accgagaccc cggccaagca gcgcatgaaa acctgtcttg accagtacaa cgccaacaag
     5641 gcggccggcg ccaatggcaa cggtggcctc aactggatcc agaagggcgg cggctactac
     5701 agccagtgca acgcccgcat gaagcagatc cagggcgtct gatcactcct tcgcgcagaa
     5761 agtcggcggg cctccctcgg ggggcccgtt gcttttgtca gaacagtttt ggcaaaaggc
     5821 ggccggtacg cgccatatag gcgcgatatt catcaccgaa cgtgtccagg agcatccgct
     5881 cttccgggcc gacgcgcagc agatacaacg tgccgaagcc gatgatgccc gatagcccgg
     5941 cgatccagtt cggcaaaagc agcgcctggg ccagggccca cagccagaag gccgaataca
     6001 ttggatgccg gatgcgggaa tagatcccct cggtaatcag cttgtgctcc ttgcgcatct
     6061 gcaacgatac cgaccagtag cgcccgagcg ccttgtgggt gagcctgaaa accaccagcg
     6121 ccgcgactgc caggacaaca ccgagccagg cagccagcgg cgaaaaggaa cgggcggcaa
     6181 aagccggcca gccggtcgcg acatagatca tcgggacaat cccgagcccg gtcagagaaa
     6241 ctgtcaggcg gagccgttcg cgggcgtcca gcgcgtcgcg agcgatcggg gtcttgcgcg
     6301 ccttgcgttc atgcggaatg cgcagcacat accagagtgc ggccatcaac acataggcgg
     6361 ccttggctat ggtaggggtc atcggcggct cccatcgacc gcagcaggtt ccagtgcggc
     6421 cttgcccgca tcggtcaagc ggccatcctc gccgatcagg cccgaaaatc cggcgtcgga
     6481 ccccgcccag acgggaagcg cgaaggggcc gcacagcatc aggtaataat catagcgggc
     6541 gcggcgatca ttggccgaga cgaattggct gtgcagacgg aagaaattgc gacggatgcg
     6601 ccgatagcga gcctcgctca gcatggtgcg gaaccggacg gaccgcatgc cggcaggccg
     6661 ggccgtcagt cccagctcgc tgaccggctc gcgacggtag aaattcatcg gatcgttgtg
     6721 ggcatggaaa tcctgccaga cgagccgcgg cgaggccgca atctcggcaa gggtcgcccg
     6781 caatcgaaac gaaccggaat ggtagccgag tttgaggatg gacgagccga gcgtcacaaa
     6841 atgcaccggc gggccctcag ggcggcgcgc cagcagcttg gcgatcaatt ccgccccgtg
     6901 cacggcgcca agactgtgcc cgacgaccag caattcgccg ccgcggctgg caagatcatc
     6961 ggcaagccgg tcgaggcgcc tgtcgacctc cgcatcaccg tgcgtcagca gccggcgggc
     7021 atagatccaa tcatccaata ggtggtccag catgagcggc cggccgatca cccgggccag
     7081 caccgcgaag gccgcgagcg ctcccacaag gcgcagaacc atggcccagc cgggccccag
     7141 aatcgccaat ggcaatgcgg cagcgatcag ccaggcgagc ccggcaagca cacccagcaa
     7201 gacgaacggg tagagaaaga acaggcaata gcgccaggca cgggtcagat aacgccagaa
     7261 cgcgccgtgc aggacgaaat ccgcatagac ccagagcgca agcggcaggc gcaggaacca
     7321 ggatcgtgat cggtcagcat cgatcagatc gttccagcag aacagataga cgtcggtatc
     7381 cgcacgccag tcggggccgg caagcgtaac cggccatcgg ctggcgtgct catgctcggt
     7441 cgctggtcct gccttggcgt tcagcgacca tgtgtccgat accgtcgcaa gatcgcgcac
     7501 aaaccgctcg tgcgccagat caggagccag cggataatat ccgccgagat aggcaaccgt
     7561 gcgctgtttt acactcccgg tcaccggctg cccctcgtcg cccattgtcc ggcgctagat
     7621 cacgccgcat tttgatggaa tcatcaaaat gcgtataacg tgatcggtat caataagtta
     7681 gagcatgtct tctgcgatta accggtatcc acttaatcgc gacatgctct cggccgcgcc
     7741 atcgttcctg tcggcgacct ttagccaagg ctggcgccgg agaaaagcct gaggttgtgt
     7801 caagcgttgg ctgcgcgacg gatcagcagc ttgtcgatcc gccggccatc catgtcgatg
     7861 acttcgatcg tccagccgtc ttccatgtcg gcggtatcac cttcaaccgg gatgcgcccg
     7921 aacagttcga gagccagtcc ggccacggta tggaagcgtg catcctccgg cacggcgatt
     7981 ccgacagttt cctccagctc ggcgatctcc gccctgccat cgaccagata ggtgccgtcc
     8041 gcgcgtgtaa cgattgcctg cctggtgacc tcgtgttctt cgggaatgtc gccggcgatg
     8101 gcttccagaa cgtcggtcag cgtcaccacg ccctcgaatg caccgaattc atcgatcacg
     8161 aaggcaaaat ggttgcgctt ggtgcggaac agttcgagca cgtcgagcac tgacgccgcc
     8221 tccggcacga acaacggctc gcgcacgttc ttctcgatgt cgaggggctt gccctcaagc
     8281 agcacatcga gcaggtcctt cttgtgcaca tagccgagcg gctcgttgat cgagccatcc
     8341 ttggccacca cgacacggct gaacggacac tggcgcaatt ccgcccggac caccttggga
     8401 tcatcttcga cgtcgatcca gtagacttcc gagcgccgtg tcatgatggc cgcgatcgag
     8461 cgttccgcca gcccgatcac ttccttgacc atcacccgct cctgcggatc gatgacgccc
     8521 gattcggtgc cttcctgcag gatggtctgc acctcctcct cggtcaccga ctggtcgcgc
     8581 accgttggca cgcgcagaag cctgagaatg aaggcggtcg acaccccgag cagcgcaaca
     8641 accggtcggg cgcccaccgc cagaagattg atcggccggg ccacccgcgc cgcaatcgtc
     8701 tcgggatagg cgagcgcgat gcgcttgggc accagttctc cgaacaccag cgtcaaaagg
     8761 gtggtgatca cgacgatgac gccgatggca attgcctcgg catgcggccg caggatttcg
     8821 aaagcggaaa ttggcgcgac cagatcattg gacagggtcg ccgtgccgaa tgcgccggtc
     8881 ccgatcgaga tcagcgtcag cccgatctgg acggaggaaa ggaaacctgt tggctccctg
     8941 gccagatcgg cggccttggc agcaccgtga tcaccagcct cggccatggc atccagccgg
     9001 gcgcgccgcg ccgagatgat cgccatttcc gacatcgcaa agaagccatt gagcagcgtc
     9061 agtgcgagaa gtacgaatat ctccagacta aacattcagc cgatcagggt taaccgccgg
     9121 tcgtgtgggc cagcaacccg cttatcgcac ggcgaaggcg tgatccgcaa ccggctcgca
     9181 cagtttgttt tcagaactga tcattctgtt ttagacgcgc ccggatcatt cgccgggtgc
     9241 tctggcctcg attgccagcg catggacgcg ctctttcaat tcatcggcga gcagcgcgtt
     9301 gatgatccgg tgtcgagcca gtcggctctg cccggcaaag gccgatgcgg tgacaatcac
     9361 acgaaaatgg gtctcgccgc cctccttcca gccggcatgg ccgtgatggc gatgactttc
     9421 gtcaatcacc tccagatgga gcggggacag ggtggcgagt ttggtggcga tgcgggtctt
     9481 gaaggtcatg gggcgtgttc taccagaatg ccggctcggg tgaaatacga ccggacaggc
     9541 ggccgcaaac cttgccccgt taaccgtcga tgcggtatcc tgtccggatg gatttcaatt
     9601 cgtcgctctt tgacaagatc cgaatcgccc gggcgcgcga agagcagcag ccggccgagc
     9661 ggcgctgcga gcatcccggc tgccagcggc cgggcaacca tcgtgcgcca atgggccggc
     9721 accgcgaggg gcgctatttc catttctgcc tcgaccatgt gcgggcctac aaccagtcct
     9781 acaattactt tgccgggatg aatgacgaat cgatccaggc ttttcagaag gacgcgatca
     9841 ctggccatag gcccacctgg tccatgggga taaagggctc acgcgggccc gaccttgagg
     9901 ccgacgacac gatctccgac atcctgggcc tgttccgcaa cggtcgccgc acaaccgaac
     9961 ggcgtgagga acggcgcgcg gtctctgccg gagcgagcaa ggccctggac gcgctcggtc
    10021 ttgaggaagg cgccggccgt gccgaggtac gggcccagtt caaatcgctt gccaagcgct
    10081 ttcatcccga cctcaacggc ggtgatcgtt cgcatgaaga aaaattgcgt gcaatcatcg
    10141 acgcctataa ttacctgaaa tccgcagggc tggcctgacc ggtcccggcc acgccaccct
    10201 ttgtgcagca tgcccggcct tctcgccggg cctctaggtg aactatgtcc gaggcagtta
    10261 ctgcgggcag cccgatgccc gataccgagt tttccgtccg cgcgctgttc aatatcgaca
    10321 gcgacatcaa ggtgcccgcc tattccaagg cgagcgatca tgtgccggaa ctcgatcccg
    10381 attatctgtt cgaccgcgat acgaccctgg cgatcctcgc cggttttgcc cgcaaccggc
    10441 gcgtcatcgt caccggctat catggcaccg gcaaatcgac tcacatcgag caggtggcgg
    10501 cgcggctcaa ctggccctgc gtgcgcgtca atctcgacag tcatgtcagc cgcatcgatc
    10561 tggtcggcaa ggatgcgatc gtgctcaagg acggcaagca agtcacagaa ttccgtgatg
    10621 gcatcctgcc ctgggcctat cagcacaatg tcgcgctggt gttcgatgaa tatgacgccg
    10681 gccgccccga tgtgatgttc gtcatccagc gcgtgcttga atcctcgggc cgtctgacgc
    10741 tgctcgacca gagccgggtg atccgtccgc acggggcgtt ccgcctgttc gccaccgcca
    10801 atacggtggg gctgggcgac acctccggcc tctatcacgg cacccagcag atcaatcagg
    10861 cacagatgga ccgctggaac atcgtcaccg tgctcaacta cctgccgcac gatcaggaag
    10921 tcggtatcgt tctggccaag gcccggcatt accgcaacaa ggaaggcaag gacatcgttt
    10981 ccaagatggt gcgtgtggcc gacctgaccc gaaacgcctt catgaacggg gagctatcca
    11041 ccgtgatgag cccgcgcacg gtcatcacct gggccgagaa cgctgatatc ttcaaggatg
    11101 tcgcctttgc cttccgtgtc accttcctca acaaatgcga tgaattggaa cgcggcacgg
    11161 tcgccgaatt ctatcagcgc tgcttcgggg tggaactgaa ggaatcgacc gtcaacgtgg
    11221 ttctgacgta aggcgggcgg caagcatgac ccggcccgtt gccctgtttt tcgacatttt
    11281 cggcacggtc gtcgactggc gcggctcaat tgcgcgtgag gcccgcgaac gatttggcga
    11341 gactttcgcg ggcgaggctt tcgccgatgc ctggcgcgac gaataccagc cggccatgga
    11401 gcgcgtacgc acgggcgggc gaggctttgt caagctcgat gtcctgcatc gggaaaacct
    11461 tgatcgcatc ctgccgcgct tcgggcttga ccggctgacc gaggccgaac gcgccgatct
    11521 caatctggtc tggcaccggc ttgatggctg gccggatgcg tcgctggcgc tgcggcgtct
    11581 gggtgtgctc taccggctgg cgccaatgtc gaacggcaat atctcgctga tggtcgatct
    11641 tgcccgccac aatcactttc actgggatgc gatcctcggg gcggaactcg cccgggacta
    11701 caagcccaaa cttgccgtct accgctccgg ctgcgacgcg ctcgatcttg cgccggaacg
    11761 ctgcatgatg gtcgctgcgc acaccagcga tctggtggcg gccaaggcgg ccggcctgca
    11821 aaccgcccat gtcgcccggc ccgatgaata cggccccggc aagggcgagg cggccccctc
    11881 cgcgccggtt gatcttgccg cgcacgatct cgaccatctg gcggagctgc tggaatgctg
    11941 agccgatcct atcatggctt gccatgcccg attccttcgg gaagaggcta ggcgatgtcc
    12001 agttccagca accgtcgccc cggccagccg gcccagcagc cgaccg
//
LOCUS       DAOMXG010000067        11926 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_2900_length_11926_cov_11.383792, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000067 DAOMXG010000000
VERSION     DAOMXG010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 11926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11926
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2900_length_11926_cov_11.383792"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..1007
                     /locus_tag="PLQ11_06225"
     CDS             162..1007
                     /locus_tag="PLQ11_06225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HRK24532.1"
                     /translation="MSLFASSRASAVEAVPEVRVNMLTMRGTLAALVARARANQGFTL
                     FTVNLDHIVKLNQNHSFREAYQRASHVTADGWPIVWLARGKDRRRVARPGARDRRQSN
                     GLERTTGADLLEPMCREAAKHGLPLYFIGPSPDSQKAGLEILKRKYPQLIVAGAETPQ
                     VGPDVDAATVEPIAERLATTKARVCIVSLGAPKQELLADRLHRACPDVGFLCVGAALD
                     FISGHAVRAPKWVRRIGMEWFWRMASDPKRLVGRYVGCGLTFFQLCLPSVFRKNPRLV
                     VAGQD"
     gene            1160..1684
                     /locus_tag="PLQ11_06230"
     CDS             1160..1684
                     /locus_tag="PLQ11_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HRK24533.1"
                     /translation="MGFTLDVAETGSVSAEKAAQRAAPRLQPGDKTRIVVYGEDRLTG
                     DYEIDGHGNIVVPLIGQVQAAGLSKKELESLLVDRLRKGQILRQPVVSVDVASFRPFY
                     VLGEVEKPGEYAYRNGLNVMSAVAVAGGYTYRASKGQVRIQRAGEKNFTDYELTPDIP
                     IYPGDLISVPERYF"
     gene            1719..3017
                     /locus_tag="PLQ11_06235"
     CDS             1719..3017
                     /locus_tag="PLQ11_06235"
                     /inference="COORDINATES: protein motif:HMM:NF021589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRK24534.1"
                     /translation="MCGALAAIAPTVAPVVATAQTVPTPPTSTSAAEETAALLAPIPH
                     SQKNPLLKPTRDYGGLPMGDWLFFPSVTAGVTFDDNLVWSSRKPVSATGFRLAPELIG
                     IRDAGIHKTTVYGEVDARLYPSVQDSTAVSARAGLAHAWEIQRDLLFKAHIDYDHRST
                     HTGSTTGSGGDFTTLAMPLAYDRVSAAASLNKSFGTMFIGLAAETVKTTYNDNATTPG
                     RAGESFRDSWTTTGTMRGGAWVSPALYVFSQVAGNIRDYGEGSDRSHGYQVVAGVGSD
                     RISLFRGEVYGGVQQQFYNSGPVKDVLVPVFGGKLFWYPTRDITLRMSLDQTFTDARL
                     VTPTNPVGHPERMTLGQISLKYQIARDWEATVRGGYSYSHFIGTGRRDQAWRAGVTVF
                     HELSRNLGLTLDYDFSRIISSQVAAGYTRNSVTLGLKYRY"
     gene            complement(3130..5424)
                     /locus_tag="PLQ11_06240"
     CDS             complement(3130..5424)
                     /locus_tag="PLQ11_06240"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRK24535.1"
                     /translation="MTLLHSLLQAQIGNCTDKSGTLDLQSLLKLISEAYDEQDSDRRR
                     TDRSINLMVEEIETANGELERLLEVKSDELTSVRLALDAAMQNTSQGLLIVDAGGRIT
                     AYNQRLIELLAFPTELLDRRPYFSELLQWQMDRGEFSMTPEELVAWIMSGGIVNSPPV
                     YERSRPDGKVLEVRTVLLKEGGAVRTFTDITERKEQLLALQRAEHLHRSLFENAAAGL
                     FRATVAGRLLDANLALAQIYGYQDVAGFLVMANSDSRWHAVDRDTRTNFREMLFASDR
                     IIDFVCEMEQPGTGHRKWISTTAWLVRDAEGAPIHYEGSVTDISERKSTEQRLAYMAT
                     HDSLTGLPNRAFLMNWLASEKGRASAQRSITIHCIDLDDFKDINDDMGHAIGDLLLKQ
                     AAERMEQLLSKGDFAVRLGGDEFAVVQIGAKSTGSVERLAARLVRCLSRDYRIGKKSL
                     VVGASVGTMTNADPRVAGEHLLKYADIALYSAKEAGGNCYRLYDSDLEARYARRRLIE
                     TSLAGALQRNEFSVVYQPILSLADGKVHHYEALLRWNHPTEGAISPAEFIPIAEQCDA
                     INALGELVLRQACSSFAMARTECSVSVNLSPVQLRQRGFIATLVDTLATTGLLPHRLT
                     LEITETVLLSDNPTTRAQLRDLRALGVRIALDDFGSGHSSLAYLKSYDFDQIKIDRSF
                     IAERELSRVNGAVINAVIQLGRELGIRVVAEGIETERQLEDLRALGCDMIQGYFIGKP
                     DAREHWPDLQPAPAEQPGSSRRVA"
     gene            complement(5421..6560)
                     /locus_tag="PLQ11_06245"
     CDS             complement(5421..6560)
                     /locus_tag="PLQ11_06245"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HRK24536.1"
                     /translation="MKTCTLTWDHQNRLRGDVAASSPDVVILFGPRELVAAPELFSDF
                     RTRFPASHVLGCSTGGQIHDGVVSDAEVVGLGLDFAETTLRFASTEIKAHAGSREAGQ
                     ALGRRLACTSLAGIFVLADGLALNGTDFVAGLAEAVGEHIPLMGGLAGDGARFEQTLV
                     IADAAGRPGIAAAVGFYGEAVRLHHGHAGGWDRFGPKRRITRSRDNILFELDGRPALE
                     LYERYLGEEAAGLPGTGLLFPLRIFNPANPDHDVVRTLLAIDREQGSVTFAGSMPQGW
                     VAQLMRGSLDRLAHGAGMAADHARIPDNWTGEGAAFLISCIGRRLLMGQRTGEEVAAV
                     RAALRRTMPVVGFYSYGEISPHLVSGFSELHNQTMTVMTITERAA"
     gene            6776..7168
                     /gene="apaG"
                     /locus_tag="PLQ11_06250"
     CDS             6776..7168
                     /gene="apaG"
                     /locus_tag="PLQ11_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HRK24537.1"
                     /translation="MYRAVTKDVEVVVRPQFSPEQSNPDKNKYFWTYAVEITNHGAET
                     IQLRHRHWEITDGNGQRQDVRGPGVVGEQPILKPGDTFRYASGCPLTTPQGIMVGEYE
                     MVAAGGSRFNVRIPAFSLDSPFEVRKVN"
     gene            complement(7330..8325)
                     /locus_tag="PLQ11_06255"
     CDS             complement(7330..8325)
                     /locus_tag="PLQ11_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone"
                     /protein_id="HRK24538.1"
                     /translation="MTVSDASVAAAGCDAADDDRILPFQVDALDVRGRVVRLGPTVDA
                     ILRRHAYPGAVSRLLGEAVALTVLIGTSLKFDGQLQLQTRSDGPVDMLVVDFSAPASV
                     RAYARFNAERLAQHPLASPAELLGRGHLAFTIDQGPHMQRYQGLVPLEGQGLEEAAHQ
                     YFLRSEQIPTRVRLAVAEEHTPDGRRWRAGGLMVQFLPGSTERQRQAEFDPGDAPAGS
                     SQPAFVEDDAWVEARSLVETVTDLELVDSCLSSESLLYRLFHERGVRVFAASSLVERC
                     RCSSERILEMLRRFTVEDRQAMIGEDGTIGVTCEFCSVHYSFRPGELDYTSGTDN"
     gene            complement(8329..9258)
                     /gene="argF"
                     /locus_tag="PLQ11_06260"
     CDS             complement(8329..9258)
                     /gene="argF"
                     /locus_tag="PLQ11_06260"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HRK24539.1"
                     /translation="MVAFRHFLDIADLQADEFLDILAAAARMKARRGTAAAAGDRPLA
                     GKKIAMVFEQPSLRTRVSFDVGIRELGGEPLMITGKEIELGERETIADTARVLSRYVD
                     AIMIRMLDHAQLTELAAHATVPVINGLTKNSHPCQVTADIMTFEERLGPLAGRTVAWT
                     GDANNVLTSWVEAATKIPFTFRIATPPDLAPRPELLAGARRKGARITLGHDPEAVVAG
                     ADCVITDCWVSMGDKDEGRRQNLLKPYQVNARLMSKASKDAIFMHCLPATRGEEVTDE
                     VMDGPQSAVFDEAENRLHAQKAILAWVFGAKVA"
     gene            complement(9276..10496)
                     /locus_tag="PLQ11_06265"
     CDS             complement(9276..10496)
                     /locus_tag="PLQ11_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRK24540.1"
                     /translation="MTKAGTKSDNSAMMGTYARIDLRFERGEGAYLITEDGRRYLDFV
                     AGVAVMSLGHSHPHLVEAVKAQAEKLWHTSNLFRIPEGERLARRLVELTFADEVFFTN
                     SGAEALECAIKTARKYHHANGQPERYRLITIEGAFHGRTLATIAAGGQQKYLDGFGPV
                     TPGFDQVPFGDLEAIKAAIGPETAGILLEPVQGEGGLRAWTPAQLRQIRDLCDERGLL
                     LVLDEVQCGMGRTGKLFSHEWSGIAPDIMAVAKGIGGGFPLGACLATHEAAKGMTAGS
                     HGSTYGGNPLAMAAGNAVLDVVAKDEFLAHVRQTALLMKQRMAELKDRFPRIIEDVRG
                     EGLLMGFKAGIPNTEVIAALREQKLLAAAAGHNVVRFLPPLIIGEAEVGAAMRALEAA
                     CASLTACAQSKPAA"
     gene            10917..11411
                     /locus_tag="PLQ11_06270"
     CDS             10917..11411
                     /locus_tag="PLQ11_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007200039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcrA family cell cycle regulator"
                     /protein_id="HRK24541.1"
                     /translation="MSWTEERIERLKKLWGDGLSASQIAAELGGVTRNAVIGKVHRLH
                     LSGRVKTSSAATQRSRKTARPVARPTAQRLVTASRGNLAVVQTLEAEEVVVYRPAAEV
                     VVPISRRITIMELREGTCRWPMGDPLTPEFVFCGGDCDIGKPYCTAHAQVAYQPSLDR
                     RRVR"
     gene            11529..>11926
                     /locus_tag="PLQ11_06275"
     CDS             11529..>11926
                     /locus_tag="PLQ11_06275"
                     /inference="COORDINATES: protein motif:HMM:NF021376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2076 domain-containing protein"
                     /protein_id="HRK24542.1"
                     /translation="MTPQEREVINGIFDRLKGAAGQPRDPEAERHIADLVRQQPYATY
                     ALAQSVYVQEQALTNMQTEIQQLQAQIGQLQQAMHQQTQQQQSGGFLSGLFGGGARPQ
                     PAPPQQQPGPWNRAPQQGMPMQSPEPPAAGP"
BASE COUNT         2312 a         3831 c         3648 g         2135 t
ORIGIN      
        1 ctgacggtca attgtcaggg ccgctggtat taccaaacga ttaatattaa aaagtattta
       61 attcgtaatt ttaacaaaag ttccccgctt aaccgcattt ttacatcgct ctcctaacgt
      121 catggccttg tagagaaccg tgttgcgttg gggcatctct catgagtttg tttgcgtcga
      181 gtcgcgcatc tgctgtggaa gcggtgccgg aagtccgcgt caatatgttg accatgcgcg
      241 gcacattggc ggctctggtc gcgagagccc gtgccaacca aggcttcacg ctgttcacgg
      301 tcaatctcga ccatatcgtc aagctgaacc agaaccattc gttccgcgaa gcctaccagc
      361 gcgccagcca tgtcacggcg gatggctggc ccattgtctg gctggcacgc ggcaaggatc
      421 gccgccgggt tgcccgacca ggtgcgcgcg accgtcgcca gtcgaacgga ttggagcgca
      481 caaccggtgc ggacctgctg gagccgatgt gtcgcgaagc ggccaaacac ggcctgccgc
      541 tgtacttcat cggccccagc ccggattcgc agaaggccgg gctggaaatc ctgaaaagga
      601 agtatccgca gctcatcgtt gccggtgcag agaccccgca ggtcggtccg gacgtcgatg
      661 ccgccacagt cgaaccgatc gccgagcgac ttgccactac aaaagcccgg gtctgtatcg
      721 tttcgctggg agcgccgaag caggaactgc tggctgaccg cctgcaccgt gcctgccccg
      781 atgtcgggtt tctctgcgtt ggcgcagcgc tggacttcat ttccggacat gctgtgcggg
      841 cgccgaaatg ggtgcgccgc atcggcatgg agtggttctg gcgcatggcc agtgacccaa
      901 aacgccttgt gggccgctac gtcggctgcg ggctgacctt tttccagctg tgcctgccgt
      961 ctgtattccg caagaacccg cggctggttg tggccgggca ggactaggca tgacctcaat
     1021 gggcatccgt tcggaacaca acgcgccagg cagcaagcac tccctgcgat ggcgcgcgtt
     1081 cgccagcaga tgggccgggc cgctgcttgc gatcgcgatt gccggcggcg ttgccggatg
     1141 tgaatccgga ctcgggtcca tggggttcac tctcgacgtt gccgaaaccg gttcggtcag
     1201 cgccgaaaag gcggctcagc gtgccgcacc gcggttgcag ccgggcgaca agacccgcat
     1261 cgttgtctat ggcgaagatc ggttgaccgg cgattatgaa atcgacggcc acggcaacat
     1321 cgtcgtgccg ctgatcggac aggtccaggc cgccggcctg tcgaagaagg aactggaatc
     1381 gctgctggtc gaccggttgc gcaaggggca gatcctgcgc cagccggtgg tcagtgtcga
     1441 tgtggcctcg tttcggccgt tctacgttct tggcgaggtc gaaaagcccg gcgaatatgc
     1501 ttatcgcaat ggcctgaacg tcatgagtgc ggttgcggtt gccggcggct acacctatcg
     1561 cgccagcaag ggacaggtgc gcatccagcg cgcaggtgaa aagaacttca ccgattacga
     1621 actcacaccc gacattccga tctaccctgg tgatctgatc agtgttcccg agcgctattt
     1681 ctgaacggcg ggaccgccgg tcctggacca gccccgcctt gtgtggagcg ctggcggcaa
     1741 ttgccccgac agtggctccg gttgtggcga ctgcccagac cgtgccgaca ccgcccacgt
     1801 caaccagcgc tgcggaagag actgccgcgc tgctggcccc catcccacac agccagaaga
     1861 atccgctgct caagccaacg cgcgactatg gcgggctgcc gatgggggac tggctgttct
     1921 ttcccagcgt gacagccggg gtgaccttcg atgacaacct tgtctggtca tcacgcaagc
     1981 cggtttcagc taccggcttc cggctggctc ccgaactgat cggcattcgc gacgccggca
     2041 tccacaagac caccgtttat ggcgaggttg atgcccggct ctatccctct gtccaggaca
     2101 gcacagcggt cagcgcccga gccggcctgg cccatgcatg ggaaatccag cgcgacctgt
     2161 tgttcaaggc ccatatcgac tacgatcacc gatcgaccca taccggcagc accacaggca
     2221 gcggcggtga cttcaccacg ctggccatgc cgctggccta tgatcgcgtc tcggccgcag
     2281 cctcgctgaa caagagcttc ggcacgatgt tcatcgggct cgctgcggaa acggtcaaga
     2341 caacctacaa tgacaatgcc acgaccccgg gccgggccgg cgagagtttc cgcgacagct
     2401 ggacgacaac cggaacaatg cgcggcggcg cctgggtttc accagcgctc tatgtgttct
     2461 cgcaggtcgc cggcaatatc cgcgactatg gcgagggcag cgaccgttcc catggctatc
     2521 aggtggtcgc cggcgtcggt tctgaccgca tcagcctgtt ccgcggcgag gtctatggcg
     2581 gtgtccagca gcagttctac aacagcgggc cggtcaagga cgtgctggtt ccggtgttcg
     2641 gcggcaagct gttctggtat ccgacgcgcg acatcaccct gcgcatgtcg cttgaccaga
     2701 cattcacgga cgcaaggctg gtgacaccga ccaatccggt cggacatccc gaacggatga
     2761 cgctgggcca gatcagcctc aaataccaga tcgcgcgcga ctgggaggca acagtccggg
     2821 gtggctacag ctattcgcat ttcatcggga ccggtcgccg tgaccaggcc tggcgcgccg
     2881 gcgtcaccgt attccatgaa ttgtcgcgca atctcgggct gacgctggac tacgattttt
     2941 cccggatcat ctccagtcag gtcgccgccg gctacacgcg gaactccgtt accctcgggc
     3001 tgaaataccg ctactaaaac cctgcggacc tgggcgggat cgcatagacg caagacctgc
     3061 cggcacagtc tcaaataaca ttggccgggg ctcgggcgcc cggctcgaaa tgcacagtag
     3121 cggcaagtct caggccactc gccgggacga tcctggctgt tccgccggag caggctgaag
     3181 gtctggccag tgctccctgg catccggttt gccgatgaag tagccctgga tcatgtcgca
     3241 gccgagtgcg cgcaggtcct ccagttgccg ttcggtctcg atgccctcgg caacaacccg
     3301 gatcccgagt tcacggccaa gctggatcac ggcattgatc accgcaccat tgaccctgct
     3361 cagttcccgc tcggcaatga aggagcggtc gatcttgatc tggtcgaagt cgtaactctt
     3421 gaggtaggcg agcgaggaat ggccagagcc gaaatcgtcg agagcaatac gcacgccaag
     3481 cgcccgaaga tcgcgcaatt gtgcccgcgt tgtcggattg tcggacagga gcacggtttc
     3541 ggtaatttca agtgtcagcc gatgcggcag caggccggtc gttgccagcg tatcgacgag
     3601 tgttgcgata aagccgcgct ggcgcagctg caccggtgaa agattgaccg agacgctgca
     3661 ttcggtcctg gccatggcaa agctggagca ggcctgtcgc aataccaatt caccgagcgc
     3721 attgatcgca tcgcattgct cggcaatcgg aatgaattca gccggagaaa ttgccccctc
     3781 ggtcggatgg ttccagcgca gcaacgcctc gtaatgatgc accttgccat cggccagcga
     3841 caggatcggc tggtagacga cgctgaattc gttgcgctga agcgctccgg ccaggctggt
     3901 ttcaatcaga cggcggcgcg catagcgtgc ttccaggtcg ctgtcataga ggcggtaaca
     3961 gttgccgccg gcttccttgg cgctatagag cgcgatgtcc gcgtatttga gcaaatgctc
     4021 gccggcaacg cggggatcgg cgttggtcat cgtgccgaca ctggcgccta ccaccagtga
     4081 tttcttgccg atgcgatagt cgcgcgacag gcagcggacg aggcgagccg ccagcctttc
     4141 caccgaaccg gtcgatttgg cgccgatctg gacaacggcg aattcgtcgc cgcccagccg
     4201 gacagcaaag tcgcccttgc tcaacagctg ctccatccgt tcggctgcct gcttgagcag
     4261 aaggtcgcca atggcatgcc ccatgtcgtc gttgatgtcc ttgaaatcgt caaggtcgat
     4321 gcaatgaatg gtgatggacc gctgagcgct ggccctgccc ttttcagaag caagccaatt
     4381 catcaggaac gcccgattgg gcaatccggt gagcgaatcg tgcgtggcca tgtaggccag
     4441 gcgctgttcg gtcgatttgc gctcgctgat gtccgtgacc gagccctcgt aatggatcgg
     4501 cgcgccctcg gcatcgcgaa ccagccaggc ggtggtcgaa atccacttgc gatggccggt
     4561 gccaggctgc tccatctcgc agacaaagtc gatgattcga tcggaggcga acagcatctc
     4621 acggaaattg gtccgcgtgt cccggtcaac ggcgtgccaa cggctatcgc tgttggccat
     4681 gaccagaaag ccggcaacgt cctgatagcc atagatctgc gcaagggcca gattggcatc
     4741 gagcaggcgc ccggcgactg tggcccgaaa caggccggcg gcggcattct caaacaggct
     4801 acgatgcaga tgttcggcgc gttgcagcgc aagcaattgc tccttgcgct cggtaatgtc
     4861 ggtgaaagta cggaccgctc caccttcctt gagcagaacc gtgcgcactt ccaggacctt
     4921 gccgtccggg cggctgcgct catagacggg cggcgagttc acaatgccgc cggacatgat
     4981 ccatgccacc agttcctccg gtgtcatgga aaattcgccg cggtccatct gccactggag
     5041 cagttcggag aagtagggac gccgatccag cagttccgtc gggaaggcga gcaactcgat
     5101 cagccgctgg ttgtaggccg tgatacgccc gccggcatcg acgatcagca acccttgcga
     5161 ggtgttctgc atcgcagcat caagcgccag gcgaaccgag gtcagttcgt cggacttgac
     5221 ctcaagcagc cgctcgagct cgccgttggc cgtctcgatc tcctcgacca tcaggttgat
     5281 cgaccggtcg gtgcggcgcc gatcgctgtc ctgctcgtca taggcttcgc tgatcagctt
     5341 taagagggac tgcagatcca gtgtcccgct cttgtccgta caattgccaa tctgcgcctg
     5401 aagaagagaa tgcagcagtg tcacgcagca cgctccgtaa tggtcatcac cgtcattgtc
     5461 tggttgtgca gctcggaaaa gcccgagacc agatgcggcg agatctcgcc atatgagtag
     5521 aagccgacga cgggcatggt gcgccgcagg gcagcccgaa ccgccgcgac ctcctcaccg
     5581 gttcgctggc ccatcagcag gcgccgaccg atgcagctga tcaggaacgc agccccctca
     5641 ccagtccaat tgtcgggaat ccgtgcatgg tccgcagcca tgcccgcacc atgcgccaga
     5701 cggtcgagcg agccacgcat caattgggcg acccagcctt gcggcatcga accggcgaac
     5761 gtcaccgacc cttgttcgcg gtcaattgcg agcaatgtgc ggacgacatc gtgatcggga
     5821 ttggccgggt tgaagatgcg caggggaaac agcaggccgg tccccggcaa tccggcggcc
     5881 tcctcgccca gatagcgttc gtagagttcc agcgccggcc ggccatcaag ttcgaacagg
     5941 atattgtcgc gcgaccgggt aatgcgacgc ttgggtccga acctgtccca gcccccggca
     6001 tggccatggt gcaggcggac cgcttcgcca tagaagccga ccgcagcggc aatgcctggc
     6061 ctgccagccg catcggcaat gacaagggtc tgctcaaagc gcgcgccatc gccggccagg
     6121 ccgcccatca gcggaatatg ctcacccacg gcttcggcaa ggcccgccac gaagtcggtg
     6181 ccgttcaacg ccagaccatc ggcaagaaca aaaattccgg cgagactggt gcaggccagc
     6241 cggcgcccca gcgcctgtcc ggcctcacgg cttccagcgt gcgccttgat ctcggtgctg
     6301 gcaaagcgca gcgtggtttc ggcaaaatca agaccgagcc caaccacctc ggcatcagaa
     6361 acaacaccat catggatctg cccgccggtt gaacagccaa gcacatggct ggcgggaaag
     6421 cgggtcctga aatctgagaa aagctcaggg gcggcaacca gttcgcgcgg accgaacagg
     6481 atcacgacat cgggcgatga tgccgcaacg tcacctctca ggcggttctg gtgatcccag
     6541 gtcagtgtgc aggttttcat atctcgcccc cctgctgggc aataaaggct ggaaacacta
     6601 aaatttgctt caaatgcaag tatttccgag tggtttgtgc cgtggacagc gccaaactct
     6661 atatatgccc aacggctgct taacgagaga accggccgcg actgatcaaa tcccgcttgc
     6721 caaacgggtg cgatgactca caattgacgg acttgaggaa acgtcgaggg ccggcatgta
     6781 tcgcgcggtg acgaaggatg tcgaggtggt ggtccggccg cagttttcgc cggagcagtc
     6841 caatcccgac aagaacaagt atttctggac ctatgccgtc gagatcacca atcacggcgc
     6901 cgagaccatc cagctgcgcc accgccactg ggagattacc gacggcaacg gccagcgcca
     6961 ggatgtgcgc ggccctggcg tcgtcggcga acagccgatt ctgaaaccgg gcgatacatt
     7021 ccgctatgct tcgggctgtc cgctgacgac gccgcaaggg atcatggtcg gcgaatacga
     7081 gatggtcgca gccggaggaa gccggttcaa cgtgcgcatc ccggcgttct cgctcgacag
     7141 ccctttcgag gtgcggaaag tcaattgacc ccggagccgg atccacttcg cttgaaagcg
     7201 ctctgatacc agtggcccgc acaaatgacc tgccggtcat ttcccgggcc gtccctcgca
     7261 accgggcctt cggccctgct cggcgtcgca ttcgctccta accagccgcc tgccaattgc
     7321 cccgaacggt caattgtcag tgccactggt ataatccagc tcgccggggc gaaagctgta
     7381 atggacagag cagaattcgc acgtcacccc gatcgtgcca tcctcgccga tcatcgcctg
     7441 cctgtcttcc accgtgaacc ggcgcagcat ttcgagaatc cgctccgacg agcagcggca
     7501 ccgttcgacc aggctgctcg ccgcgaacac acggacgccg cgctcatgga acagacgata
     7561 gagcagcgat tccgacgaca ggcaggaatc gaccagttcg agatccgtca cagtctcgac
     7621 cagcgagcgg gcctcgaccc aggcatcatc ctcgacaaag gccggctgcg agctgccggc
     7681 cggcgcatcg ccggggtcga attccgcctg tcgctgccgt tccgtcgagc cgggcaggaa
     7741 ctgcaccatc agcccgccgg ctcgccagcg gcgtccgtcc ggagtgtgtt cttcggcaac
     7801 agccagccgc acgcgggtcg gaatctgctc ggagcgcagg aaatactgat gcgcggcttc
     7861 ctcaagtccc tgtccttcca gcggcaccaa tccctggtac cgctgcatat gcgggccctg
     7921 atcgatggtg aaggccagat gccccctgcc aagcaactcg gcgggagagg ccagcggatg
     7981 ctgcgccaga cgttcggcgt tgaagcgggc gtaggcgcga accgaggcag gcgcactgaa
     8041 atccaccacc agcatgtcca ccggaccgtc actgcgggtc tgcaactgca actggccatc
     8101 gaatttcagc gatgtgccga tcagcaccgt aagggcgacc gcctctccca gcaggcgcga
     8161 gaccgccccg ggataggcat gacggcgcag gatggcatcg accgtgggac caagcctgac
     8221 cacgcgcccg cgcacgtcca gcgcatccac ctgaaacggc agaatccggt catcgtccgc
     8281 ggcgtcgcag ccagccgctg cgaccgaagc atcgctcacc gtcatggatc aggccacttt
     8341 ggcgccaaac acccaggcaa ggatggcctt ctgggcatgc agccggttct cagcctcgtc
     8401 gaacaccgcc gattgcggtc cgtccatgac ctcgtcggtc acttcctcgc cgcgggtcgc
     8461 cggcaggcaa tgcatgaaaa tcgcgtcctt gctggccttg gacatcagcc gggcattgac
     8521 ctgatagggt ttcagcaggt tctgccggcg gccctcatcc ttgtcgccca tcgacaccca
     8581 gcaatcggta atgacacagt cagcgcccgc caccaccgct tccggatcat gaccgagggt
     8641 gatgcgtgcg cccttgcggc gggccccagc gagcaattcg ggacggggag ccagatccgg
     8701 cggggtggcg atccggaagg tgaaggggat cttggtcgcc gcctcgaccc atgaggtcag
     8761 cacattgttg gcgtccccgg tccaggccac cgtgcggccg gcaagcgggc cgagccgctc
     8821 ctcgaaggtc atgatgtcgg ccgtgacctg acaggggtgc gagttcttgg tcaatccatt
     8881 gatcaccggc accgtcgcat gggcagccag ttccgtgagc tgcgcatggt cgagcatccg
     8941 gatcatgatg gcgtcgacat agcgcgacag aacccgggcg gtgtcggcga tggtttcgcg
     9001 ctcgccgagt tcgatctcct tgccggtgat catgaggggc tcgccaccca gttcacggat
     9061 gccgacatca aacgagacgc gcgtgcgcaa cgatggctgc tcgaacacca tcgcaatctt
     9121 cttgccggcc agcgggcgat cgcccgccgc cgccgccgtg ccacgccgcg ccttcatgcg
     9181 cgcagccgca gccaggatgt ccaaaaactc atccgcctga agatcggcga tatcgagaaa
     9241 atgccggaac gcaaccatgg acactcacct gatcttcatg ctgccggctt gctctgggcg
     9301 caggcggtca ggctggcgca ggccgcctcc agcgcgcgca tcgccgcacc aacctccgcc
     9361 tcgccgatga tcagcggcgg aaggaagcgc acaacattgt ggccggccgc tgcggccagc
     9421 agcttctgtt cccgcagagc cgcgatgacc tcggtattgg gaatgccggc tttgaacccc
     9481 atcagcagcc cctcgccgcg cacatcctcg ataatgcggg gaaagcggtc cttgagttcg
     9541 gccatgcgct gtttcatcag caatgccgtc tgccgcacat gggccaggaa ttcatccttg
     9601 gcaacaacgt cgagcacggc attgccggca gccatggcga gcgggttgcc gccataggtc
     9661 gagccatgcg agccggcagt catccccttg gcagcctcat gggtcgcaag gcaggcgccg
     9721 agcgggaagc ccccgccgat gcccttggca accgccatga tgtccggcgc gatacccgac
     9781 cattcatgcg agaacagctt gccggtgcgg cccatgccgc actgcacctc atccagcacc
     9841 agcaacaggc cgcgctcgtc gcacaggtcg cgaatctgac gcaactgggc cggcgtccag
     9901 gccctaagcc ccccctcgcc ctgcaccggt tcgagcagaa tgcctgctgt ctccgggcca
     9961 atggcggcct tgatcgcctc cagatcgccg aacggcacct gatcgaaccc gggcgtgacc
    10021 gggccgaagc catcaagata cttctgctgc ccgcctgcgg cgatggtcgc cagggtgcgg
    10081 ccatggaacg cgccttcaat ggtgatcagc cggtagcgtt ccggctggcc attggcatga
    10141 tgatacttgc gcgcggtctt gatggcgcat tcaagcgcct cggcgccgga attggtgaag
    10201 aacacctcat cggcgaaggt cagctcgacc agacgccggg ccagccgctc cccttcggga
    10261 atgcgaaaca ggttcgatgt atgccatagc ttttcggcct gcgccttgac cgcctcaacc
    10321 agatgcggat ggctgtggcc aaggctcatc acggcgacgc cggcaacgaa atcgaggtat
    10381 cgccggccgt cctcggtgat cagataggcg ccttcgccac gctcgaacct cagatcgatg
    10441 cgtgcatagg tccccatcat cgccgaattg tcggacttgg tgccggcctt ggtcatctct
    10501 gcctcacgtt ccaaacgcct gaagaaacgc aaaaagccgc ccacctcggg cgggggcggc
    10561 gctctgcttg ttccatgact tggcgccggc gtcaattata cgcaaggggt ggcaaaccat
    10621 gacatgatgt gaccgacagg atcagccgcg ctcatgcgtg ccgtcgcagg aatgccgcag
    10681 gcaagatcga ctcttgacga gaccggaact ttcccacagg agatcgctgg ggaaaactgg
    10741 cgcaagcggt gtgcggaatc tgccgctttc gtccctgatc tagtagcgtc cagttcgaac
    10801 gacacgagat gtcgtagttc gtcgcgtgat ttcagtttgt gcgtggattt gccgccggga
    10861 ggccgaactt cccgggagag cctgattcat gcatgcgctg gtgttaggga tgcatcatgt
    10921 cttggaccga agaacggatt gaacggctta agaagctctg gggcgacggg ctcagcgcaa
    10981 gccagatcgc agccgaactg ggcggcgtta cccgcaatgc cgtgatcggc aaggtgcacc
    11041 ggctgcatct gtcgggccgg gtcaagacgt cgagtgcggc cacccagcgc tcgcgcaaga
    11101 ccgcccgccc ggtcgcccgg cccacagccc agcgactggt gaccgcctca cgtggcaatc
    11161 tggcagtcgt gcagacgctt gaagccgagg aagtggtggt ctatcgccca gccgccgaag
    11221 tggtggttcc aatttcccgg cgcatcacca tcatggaatt gcgggagggc acctgccgct
    11281 ggccgatggg tgatccgctg acgccggaat tcgtgttctg cggtggcgat tgcgatatcg
    11341 gcaaacccta ttgcaccgcc catgcgcagg tggcctacca gccgtcgctg gatcggcgaa
    11401 gggtccgctg accagaaaaa aaggcggtat caggtctgga aattgccatg gttatgacca
    11461 cctaatgctt cgtccgcatc cggcgaggcg atggccgccc ccggcggcga acaggtggag
    11521 gatttcccat gaccccgcaa gaacgcgagg taatcaacgg catttttgat cgtctcaagg
    11581 gcgccgcggg ccagccgcgc gatcccgagg ccgagcgtca catcgccgat ctcgtgcgcc
    11641 agcagcctta tgccacctat gccctggccc agtcggtcta tgtgcaggag caggcgctca
    11701 ccaacatgca gaccgagatc cagcaattgc aggcccagat cggccagttg cagcaggcaa
    11761 tgcatcagca gacccagcag cagcagtccg gcggcttcct ctccggcctg ttcggcggcg
    11821 gcgcacgtcc gcagcctgcg ccgccgcagc agcagccagg gccatggaac cgtgcacccc
    11881 agcagggaat gcccatgcaa tcgcctgaac cgccggctgc cggccc
//
LOCUS       DAOMXG010000068        11847 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_2922_length_11847_cov_11.942419, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000068 DAOMXG010000000
VERSION     DAOMXG010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 11847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11847
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2922_length_11847_cov_11.942419"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="PLQ11_06280"
     CDS             complement(<1..218)
                     /locus_tag="PLQ11_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24543.1"
                     /translation="MRFLALLIAGLSLAAAAQSQWMPTAGAPIYLAITGLMVALLLLR
                     AVVLGPFLKFFSVFYALSFALLLALTMLA"
     gene            complement(516..2153)
                     /gene="groL"
                     /locus_tag="PLQ11_06285"
     CDS             complement(516..2153)
                     /gene="groL"
                     /locus_tag="PLQ11_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010682003.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HRK24544.1"
                     /translation="MAAKDVRFSSDARERMLRGVDILANAVKVTLGPKGRNVVIDKSF
                     GAPRITKDGVTVAKEIELADKFENMGAQMVREVASKTNDIAGDGTTTATVLAQSIVRE
                     GAKYVAAGMNPMDLKRGIDKAVAAAVEDIKKRAKKVKDSSEVAQVGTISANGDKHIGE
                     MIANAMQKVGNEGVITVEEAKTADTDVDIVEGMQFDRGYLSPYFVTNAEKMVAEFEDP
                     YVLIFEKKLSSLQPMLPILEAVVQTGKPLLIIAEDVEGEALATLVVNRLRGGLKVAAV
                     KAPGFGDRRKAMLEDIAILTGGNMISEDLGIKLENVTLPMLGRAKRVRIEKENTTIID
                     GAGKKKDITDRVAQIKAQIEETTSDYDREKLQERLAKLAGGVAVIRVGGATEVEVKEK
                     KDRVDDALNATRAAVEEGIVPGGGCALLRAKGAVAKVSADNEDEKAGIAIVLKALEAP
                     IRQIVENSGVEGSIVVGKIAENKSETFGFNAQTEEYVDMIKAGIVDPAKVVRTALQDA
                     ASVAGLLITTEAMIAEAPKKDAPAPAIPGGGMGGMDF"
     gene            complement(2203..2490)
                     /gene="groES"
                     /locus_tag="PLQ11_06290"
     CDS             complement(2203..2490)
                     /gene="groES"
                     /locus_tag="PLQ11_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HRK24545.1"
                     /translation="MKFRPLHDRVVVRRLNSEEKTKGGIIIPDTAKEKPQEGEIIAVG
                     SGARDESGKLIPLDVKKGDKVLFGKWSGTEVKIDGEELLIMKESDIMGIIG"
     gene            complement(2688..3521)
                     /locus_tag="PLQ11_06295"
     CDS             complement(2688..3521)
                     /locus_tag="PLQ11_06295"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRK24546.1"
                     /translation="MPIVLCLVAIGLLTLMNGFIKALTGRYDPLEIAFLRYVVGGSWA
                     LLATAWIRPGWPERSILLPQIARGTLGMVSGTSFFFALRDLPLADTFTISFLAPLFVT
                     ILAMLFLKERPRALDIVALLLGFAGVLVIALGVGGSGAGRPLFGMSAAMLSAITYAIS
                     IVMLRSLAQSQPHIHMVLFQHWVSALWLLPAVLMVWKTPELADLPVLLLAPLLGVAGH
                     LLMTHAYARMPAARLAPTEYSALIYAITLDSLWFSTSPANDMLLGAIAIILAAALAAR
                     R"
     gene            3930..4553
                     /locus_tag="PLQ11_06300"
     CDS             3930..4553
                     /locus_tag="PLQ11_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HRK24547.1"
                     /translation="MNDNPVGAARPGPVCRGIVWLSARLLAAERLAVTGFMVLLTGLI
                     LLNVVTRYTGIPLYWVDESAVYSVVFLTFIGASAMTRLRLDFAVTMLTERFSERGIRI
                     AKVIATSIVLLFGLSLLWMCVLWLDPVGIARAGFNAKDFAGESFNFVYTERTQTLNWP
                     FWVLYLIMPVFALSMTVHSAANLLEDLEWVPRADHADFIKSGVEGMN"
     gene            4555..>5565
                     /locus_tag="PLQ11_06305"
     CDS             4555..>5565
                     /locus_tag="PLQ11_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010975203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRK24548.1"
                     /translation="MLTGATFLAAMLIGVPIGLCLCIGGIAFILASGNPILFQSFPLT
                     MFGGVDSYGLIAIPLFILIGEIMNGGGITRRIVDLAMAFVGALKGGLAYVNILANMFV
                     ASILGSATAQVAIMSQIMVPEMEKQGYDKDFAAGLTAYGGMLGPIIPPSVMFVVYSVM
                     AQVSVSDMLIAGIVPGIVLTMLFFAVIALMGFVYNYPHAQRRTPRERLATILRAAPTM
                     LIPIVIVGSILTGLANATESAAVGALAAALIGRYWTKELEFKRLPEMMLRAGIYSAIV
                     LFLVAAAAVFSWVLIYGKIPQMAASWVQTVAKDPVSFMLLTNVILLPIATEVYGIDPR
                     HFG"
     assembly_gap    5566..5665
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5666..5915
                     /locus_tag="PLQ11_06310"
     CDS             <5666..5915
                     /locus_tag="PLQ11_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRK24549.1"
                     /translation="ILLPIATEVYGIDPRHFGVVVVINLVLGLLSPPVGLCFFVAAAI
                     TGARPGKMFMVTLPFFGVSCMLLILLSIYPWLSLVLLK"
     gene            5943..6926
                     /locus_tag="PLQ11_06315"
     CDS             5943..6926
                     /locus_tag="PLQ11_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRK24550.1"
                     /translation="MSLSRRQILAIGASLPVAAPTVLLAQARELRLGIITPVGHSWNK
                     AALALGDKLKAASNGRLTLTVFHSGQLGNEPAMMQQLQSGALDMGFIQAAELGSRVPH
                     LAAINAPYIVRSTPAVAKFVRHPEVLKLFDVLPKETGTIGLGWGITGMRAIFSSRDLQ
                     SLADIKGMKLRINPTPVYRDFYQLLGAAPTPIPTPQVFDAMANGQVDGLEADLEFSWN
                     QRFDKVSKVILQMNAVFMPFAAIVSGRVWQTMPPADRELITKLVKETLDAQIDELAGN
                     EPNLIENFKKAPIPIRQVAVADTEAVIAEFDKIWMPQAPVIANLRKVGASL"
     gene            6995..7933
                     /locus_tag="PLQ11_06320"
     CDS             6995..7933
                     /locus_tag="PLQ11_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018064681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRK24551.1"
                     /translation="MSKTVFSGVMPALMTPCRADRRPDYDALVRKGRELVAAGMSGVI
                     YCGSMGDWPLLTDEERMTGTERLVEAGLHVIVGTGAQNPMRAAALAAHAKKIGAAGLM
                     VIPRVLSRGSSPAAQYAHFAGILEAAVDLPSVIYNSPYYGFETKADLFFALHDRYPHL
                     VGFKEFGSARAMSYAAEHITGRSAALTLMVGVDTEVFHGFVNCGATGAITGIGNVLPR
                     EVLHLARLCERAACGDVVARRQAAELEAAMKVLSTFDEGADLVLFYKYLMVLEGNPEY
                     ELHFNPTDALSESQKTFARTQLGLFKTWYASWSSAN"
     gene            7954..9057
                     /locus_tag="PLQ11_06325"
     CDS             7954..9057
                     /locus_tag="PLQ11_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HRK24552.1"
                     /translation="MKITAIRFWQVDLPLREGRYNWSNGNFVEVFDSTVVAVDTDAGI
                     TGYAECCPLGSAYLPAYALGVRSGLAELGPKLIGLDPTDLGTLNRHMDAVLRGHPYIK
                     APIDIACWDILGKATGLPVYKLLGGAAQDRIALYRAISQESPEAMAAKIAGYKKEGYT
                     KFQLKVGGNADTDIVRIHETRAILEAGDILVADANTGWTRAEAARIAAAVANVDVYIE
                     QPCPTYEECLSVRRRTVRPFVLDEVIGDLGTLMTALADDAMDIINLKISKVGGLTKAR
                     LLRDICVATGTPMTIEDTWGGDIVTATIAHLAISTPEEFCFSATDFNSYGTVDIADGA
                     PMRVNGFMTASDTPGLGVSPRLDVLGKPSGMIR"
     gene            9091..10107
                     /locus_tag="PLQ11_06330"
     CDS             9091..10107
                     /locus_tag="PLQ11_06330"
                     /inference="COORDINATES: protein motif:HMM:NF014653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HRK24553.1"
                     /translation="MTVAEAEAFAINILSANGLVEHQAGPIARAMALAEAAEVRSHGL
                     YRLIGYVGSIRSGRVNPSARPQLQTIAGSFLRVDGDNGFAPAAHGIGAPALIEAARTN
                     GIAALALTNCYHFSALWHDLDPLVNAGLACWAFTLGQCIVAPHGGTRRLMGTNPIAFG
                     WPREGEDPFLFDFATSVVARGEIELMRRAGKDLPEGWGLDAAGMPTRDPQAALAGALL
                     PFGGYKGSALSMMVELLAGPLIGEPTSLEAAALDNGDGGPPLGGELLIAMSPDLFNRG
                     LANDWRQQAEMFFAEARKQPGLRLPSDRRHGALRHNAEAGIRISKALLEELTNLASPG
                     LQYY"
     gene            10153..11712
                     /locus_tag="PLQ11_06335"
     CDS             10153..11712
                     /locus_tag="PLQ11_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24554.1"
                     /translation="MVYDIAWNSAGTTTLSPLRDMRAEHAFASAVSVSNAASVKEPDP
                     GKPTKTLSQYADGVIDWWSGVLKGDFEENPTIGQTVTRSLLTMIPFVDQGGDIQDLTA
                     NLYKCYQKGRIEASDGLAIALTAVGAIPELGSALKGSFKIILQASKELAANKAGDAVA
                     LARKLFAMPMVRNSIDRFLANAQDLRKDMIAGINTALDQIAKLNPDLAGYVARKRASL
                     VEAAAEGFDALRDTLADTKKRLMAPIRQTPAAPAGYHAPGPTPPGNLPSFTPQKYFTG
                     AIKPAIRDYPWGKAVHIDAFPANLSIQDRALYIRDAMNVAGIDQVVLPKGGDKGQIMA
                     ALQLMEGREFLYARALDGAHSAERVIVRGNDYSVRLPSFDLVPIAHTHPPSSLEKWFG
                     EFISVMPSDKDIEVLRESGGYVSSTIISTAGQARRHFDFRQILANPRMKASLAKLSMS
                     GRQAMRQHIVEQKLAYEEMIFQFGRASDLVGPKHKRYSEFLTHMTSFNELIKDCNEME
                     KLINQLPRTVR"
BASE COUNT         2191 a         3570 c         3612 g         2374 t
ORIGIN      
        1 gccagcatcg tgagcgccag caacagagca aaggacagag cgtagaatac cgagaagaat
       61 ttgaggaatg gtccgagaac aacggcgcgc agcaacagca gcgcaaccat gagccccgtt
      121 atcgccagat agatcggcgc gccggccgtc ggcatccatt gggactgggc tgcggccgca
      181 aggctgaggc cggcaatcag gagagcaaga aaacgcatgc gggtccttcg tcggaaacgc
      241 tcgggccaga atcaaccaag tgatcctaaa cacgccggcg aacgcatcgg aatcacattc
      301 tgtaatggca aaggcccggc gtcgccgccg ggccttgctg catggatcga tgggatcgct
      361 gaaaattcag aaatccaatc cgccactgaa gtcggctgtt gccgccttca gacccagtat
      421 cgccttgggc ggcggacttc ttagaagtcc atgccgccag tgaagtcggc tgttgccgcc
      481 ttcagaccca gtatcgcctt gggcgaatgg acttcttaga agtccatgcc gcccatgccg
      541 ccgcccggaa tcgccggagc cggcgcgtcc ttcttcggtg cttctgcgat catcgcctcg
      601 gtggtgatga gcaggccggc gaccgaagcg gcgtcctgaa gggcggtgcg cacgaccttg
      661 gccggatcga cgataccggc cttgatcatg tcgacatatt cttcggtctg ggcattgaag
      721 ccgaaggtct cggacttgtt ctcggcgatc ttgccgacga cgatcgagcc ttcgacaccg
      781 gaattctcga cgatctggcg gatcggcgcc tcgagcgcct tcagcacgat cgcgataccg
      841 gccttctcgt cctcgttatc ggccgagacc ttggcaacgg cgcccttggc gcgcagcagg
      901 gcgcagccgc cgccgggaac gatgccttct tccaccgcag cgcgggtggc gttgagcgcg
      961 tcgtccacgc ggtccttctt ctccttgacc tcgacctcgg ttgcgccgcc gacgcggatc
     1021 accgcgacgc cgccggcgag cttggccaga cgctcctgca gcttctcgcg gtcgtagtcc
     1081 gaggtggtct cctcgatctg cgccttgatc tgggcaacac ggtcggtgat gtccttcttc
     1141 ttgccggcgc cgtcgatgat cgtggtgttc tccttctcga tgcggacgcg cttggcacgg
     1201 ccgagcatcg gcagggtgac gttctcgagc ttgatgccga gatcttccga gatcatgttg
     1261 ccgccggtga ggatggcaat gtcttccagc atggccttgc ggcgatcacc gaagcccggc
     1321 gccttgacgg ccgccacctt gaggccgccg cgcaggcggt tgacgaccag ggtggccagc
     1381 gcctcgccct cgacgtcctc ggcgatgatc agcagcggct tgccggtctg caccaccgct
     1441 tcgaggatcg gcagcatcgg ctgcagcgag gacagcttct tctcgaagat caggacgtag
     1501 ggatcctcga actcggccac catcttctcg gcgttggtga cgaaataggg gctgagatag
     1561 ccgcggtcga actgcatgcc ctcgacgatg tcgacgtcgg tatcggcggt cttggcttcc
     1621 tcgacggtga tgacaccctc gttgccgacc ttctgcatcg cgttggcgat catctcgccg
     1681 atatgcttgt cgccattggc cgagatggtg ccgacctggg ccacctcgct cgaatccttg
     1741 accttcttgg cgcgcttctt gatgtcctcg acggcggcgg cgacggcctt gtcgatgccg
     1801 cgcttgaggt ccatcgggtt catgccggcg gcgacatact tggcgccttc acgcacgatc
     1861 gactgggcga gaacggtggc ggtggtggtg ccgtcaccgg cgatgtcgtt ggtcttggag
     1921 gcaacctcgc ggaccatctg ggcgcccatg ttctcgaact tgtcggcaag ctcgatctcc
     1981 ttggcgacgg tcacgccgtc cttggtgatg cgcggggcgc cgaacgactt gtcgatcacc
     2041 acattgcggc ccttggggcc aagcgtgacc ttcacggcat tggcgagaat gtcgacacca
     2101 cgcagcatgc gctcgcgggc atcggaggaa aagcggacgt ctttggcggc cattgtgaaa
     2161 aatcctgatg ttggtgtttg tgatgagagg acgtaagcgg gctcagccga tgatgcccat
     2221 gatgtcggac tccttcatga tcaggagctc ttcgccatcg atcttgacct cggtgccgga
     2281 ccacttgccg aacagcacct tgtcgccctt cttcacatcg agcgggatca gcttgccgga
     2341 ttcatcgcgc gcgccggagc cgacggcgat gatctcgccc tcttgcggct tttccttggc
     2401 ggtatccgga atgatgatgc cgcccttcgt cttttcctcg ctgttcaggc gacggacgac
     2461 gacgcggtcg tgcaggggac gaaacttcat gggttcactt ccttcttgac tggatcgttc
     2521 ccgaagccgg tgtctgagtg ccaagagccg ccctcttcga ggcgcggctg ttagcactct
     2581 ccggccgaga gtgctatcaa cagaggcgga gatagtgagg ccctttgcct gagtcaagcg
     2641 ctgctgcgac cttcgtcgaa gggcgactgg ctggttctgg ccgatggtta acgccgcgcc
     2701 gccagcgcgg cggccagaat gatggcgatc gcgcccagca gcatgtcgtt ggccgggctg
     2761 gttgaaaacc acaggctgtc gagcgtgatc gcatagatca gagcggagta ttcggtcggg
     2821 gccagccgtg cagcgggcat gcgcgcatag gcatgggtca tcagcagatg gcctgcgacc
     2881 cccagcagcg gcgccagcag caaaaccggc agatcggcaa gctccggcgt cttccacacc
     2941 atcagcacag caggcagcaa ccagagggcg gaaacccaat gctggaacag caccatatga
     3001 atatgcggct ggctctgcgc cagcgaacgc agcatcacta tcgagatcgc ataggtaatg
     3061 gccgaaagca tcgccgcgct catgccgaac agcggccggc cggcgccgct gccaccaaca
     3121 cccagggcga tcaccagaac accggcaaag cccagcagca atgccacgat gtcgagcgcc
     3181 cttggccgct ccttgagaaa cagcatcgcc aggatggtca cgaacagcgg cgcaaggaac
     3241 gagatggtga acgtgtcggc gagcggcaga tcgcgcaaag cgaagaagaa cgaggtcccg
     3301 gaaaccatgc ccagcgtgcc gcgcgcaatc tgtggcaaca ggatggagcg ttctggccag
     3361 ccggggcgga tccaggccgt tgccagcaaa gcccaggagc cgccgacgac atagcgcaga
     3421 aaggcaatct caagcgggtc atatcgacct gtcagcgcct tgatgaagcc gttcatcagc
     3481 gtcagcagcc cgatcgcgac cagacaaagc acgatgggca cgcccgctcc ccctcaatgc
     3541 ctgccccgat ggcggcatgc gcgcagccgg atcgctccgg agccgactca accagacgtt
     3601 catgctggca ccgatcctca tcccgttcaa tgcgctgggc gttttggcgc cggctgagag
     3661 gacagcggat ccgtgtccga tgtggtcgat ccttggtttg ctgcctgcgc atccattgat
     3721 ctggaatccc tgactgcagc aatggtcctg cggttctgtc cggcggcctg ccatcggacc
     3781 gaaggggcac ggcctcgtgc tcctgcgccc gcttcgtcaa ggattgccat cggtatcgaa
     3841 tcctgcggcg tcacgggcgg catatccggt ttgacaatca gattttatac aatatacctt
     3901 ttaggacaaa agacctctgg ggaagggcaa tgaacgacaa tccggtcgga gctgcgagac
     3961 cagggcccgt ttgccggggc attgtctggc tttcggccag gcttctggca gccgagcgtc
     4021 tggccgtaac gggcttcatg gtcctgctga ccggcctcat cctgttgaat gtcgtcaccc
     4081 gctacacagg aattccgctc tattgggttg atgaatcggc ggtctattct gtggtattcc
     4141 tgacgttcat cggcgcatcc gcgatgacac gcctgcggct ggattttgcc gtcacgatgt
     4201 tgaccgagcg cttttccgaa cgcggaatac ggatagccaa ggtcattgcc acctcgattg
     4261 tgctgctgtt tggcctgtcc ttgctgtgga tgtgcgtgtt gtggcttgat ccggtcggca
     4321 tcgcccgggc cggcttcaat gcgaaggact tcgctggcga gagcttcaat tttgtctaca
     4381 cggagcgcac gcagacgctg aactggccat tctgggtgct ttatctgatc atgccggtct
     4441 ttgccctgag catgacggtc cacagtgccg ccaatcttct tgaggacctc gaatgggttc
     4501 cccgggcgga ccatgccgat ttcatcaagt ccggtgtgga aggcatgaac tgacatgctg
     4561 accggagcaa ccttccttgc ggcgatgctg atcggagtac cgatcgggct ttgcctgtgc
     4621 atcggcggca ttgccttcat ccttgcttcg gggaatccaa ttctgttcca gtcatttcct
     4681 ctgacgatgt tcggcggcgt cgacagctat gggctgatcg ccatccccct gttcatcctg
     4741 atcggcgaga tcatgaacgg tggcggcatc acccggcgga ttgtcgatct tgccatggcc
     4801 ttcgtcggtg cgctgaaggg cggccttgcc tatgtgaaca tcctcgccaa catgtttgtc
     4861 gcctccattc tcggctcggc cacggcccag gtcgcgatca tgtcgcagat catggtgccg
     4921 gagatggaaa agcagggcta cgacaaggat ttcgctgccg gcttgaccgc ctatggcggc
     4981 atgctggggc cgatcattcc gccatccgtg atgtttgtgg tctattccgt gatggcgcag
     5041 gtatcggtca gcgacatgct gattgcaggg attgttccgg gcatcgtcct gacaatgctg
     5101 ttcttcgccg tgattgcgtt gatgggcttt gtctacaatt atccgcatgc acagcgccgg
     5161 acgccgcgcg agcgcttggc cacgatcctg agggcggcgc caaccatgct gatcccgatc
     5221 gtcatcgtcg gctcgattct caccggtctt gccaatgcga cggaatcagc ggcagtcggc
     5281 gcgctggctg cggcgctgat cggcagatac tggaccaagg agctggagtt caagcggctg
     5341 cccgaaatga tgctgcgggc cggaatctat tcggcgatcg tgctgtttct ggtggctgct
     5401 gctgcggtgt tttcctgggt gctgatctac ggaaaaattc cgcagatggc cgcctcctgg
     5461 gtgcagacgg tggccaagga cccggtcagt ttcatgttgc tgaccaatgt gatcctcttg
     5521 ccgatcgcga cagaggtcta tgggatcgac ccacggcatt tcgggnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnngatcc tcttgccgat cgcgacagag gtctatggga
     5701 tcgacccacg gcatttcggg gtcgtcgtcg tcatcaatct cgttctcggg ttgctctcgc
     5761 caccggtcgg cctgtgcttt ttcgtggcgg cggccatcac cggcgccagg cccggcaaga
     5821 tgttcatggt gacactgccg ttctttggcg tgtcctgcat gctgctcatc ctgctgtcga
     5881 tctatccgtg gctgtcgctt gttctgttga aatgatgcgg actaacaacg aggaggaatg
     5941 tcatgtcgct atctcgccgt cagattcttg ccatcggtgc atccctgccg gtcgctgccc
     6001 ccacggttct gttggcgcag gccagggagc tgcgcctggg gatcatcacc ccggtcgggc
     6061 attcctggaa caaggccgcg ctggcgctcg gtgacaagct caaggctgcc agcaatggcc
     6121 ggctgacgct gacggtgttc cactccggac agcttggcaa cgaacctgcc atgatgcagc
     6181 agctccagtc cggcgcgctc gatatgggct tcatccaggc ggccgagctc ggctcgcgcg
     6241 tgccgcatct cgccgcgatc aatgcgccgt atatcgtgcg ctccaccccg gccgtcgcca
     6301 agtttgtccg ccaccccgag gtgctcaagc tgttcgatgt cctgccgaag gagaccggca
     6361 cgatcggcct tggctggggc attaccggca tgcgggcgat cttctcctcg cgtgatctgc
     6421 aatcgctggc cgacatcaag gggatgaagc tgcgcatcaa tcccacgccg gtctatcgcg
     6481 acttctacca gctgctcggt gcggcgccca cgccgattcc gacgccgcag gtgttcgacg
     6541 ccatggccaa cggtcaggtt gatggtctgg aagcggatct tgaattttcc tggaaccagc
     6601 ggttcgacaa ggtctccaag gtcattctgc agatgaatgc ggtgttcatg ccgtttgccg
     6661 ccatcgtttc cgggcgcgtc tggcagacca tgccgccggc cgaccgggag ctgatcacca
     6721 aactggtgaa ggagacgctt gacgcccaga tcgatgaact tgccggcaat gaaccgaacc
     6781 tgatcgagaa cttcaagaaa gctccgattc cgatccgtca ggtcgccgtt gccgataccg
     6841 aagcggtcat tgccgaattc gacaagatct ggatgccgca ggcgccggtc atcgccaacc
     6901 tgcgcaaggt cggggcctca ctctgacgtg tagggacgtt tgctccgccg cctggcgcgg
     6961 cggagctacc ccttttttct gaaagtatga atcgatgagc aagaccgtgt tttccggcgt
     7021 gatgccggca ttgatgacgc cctgccgcgc agatcgtcgc cctgactacg atgcactggt
     7081 gcgcaagggc agggaactgg tcgcggcagg aatgtcgggc gtgatctatt gtggctcgat
     7141 gggcgactgg ccgctgctga ccgacgaaga gcggatgact ggcaccgaac gactcgttga
     7201 ggcagggctg catgtgattg tcggcaccgg ggcgcagaac ccgatgcggg ctgccgccct
     7261 cgccgcccat gccaagaaga tcggtgcggc cggtttgatg gtgattccgc gtgtgctctc
     7321 gcgcggctcg tctccggcgg cccaatatgc ccatttcgcc ggcattctgg aggcggcggt
     7381 tgatctgccc tcggtgatct acaacagccc gtactacggc tttgaaacca aggcggatct
     7441 gtttttcgcc ttgcatgacc gatatccgca tctggtcggc ttcaaggaat tcggcagcgc
     7501 cagggcgatg agctatgcgg ctgagcatat taccgggcgg tctgccgcgc tcaccctgat
     7561 ggtcggtgtc gataccgagg tattccacgg ctttgtcaat tgcggtgcga ccggcgccat
     7621 caccggcatt ggcaatgtcc tgcccaggga agtgctgcat ctggcgcggc tctgcgagcg
     7681 cgctgcctgc ggcgatgtgg tggcccgccg gcaggcagca gagctcgaag cggcgatgaa
     7741 ggtgctctcg accttcgatg agggggccga tctggtgctg ttctacaagt atctgatggt
     7801 gctggagggc aaccccgaat acgaattgca tttcaatccc accgatgcgc tgtccgaaag
     7861 ccagaaaacc ttcgcaagga cccagcttgg gctgttcaag acctggtatg catcctggtc
     7921 gagcgccaac tgaataccgg gaaggagcag gccatgaaaa tcaccgcaat ccgcttctgg
     7981 caggttgatc tgccgctgcg cgaaggccgc tacaactggt ccaatggcaa ttttgtcgag
     8041 gtgttcgact cgaccgttgt cgctgtcgat accgatgccg gcatcaccgg ctatgcggaa
     8101 tgctgcccct tgggttctgc ctatctgccc gcctatgcgc tcggggtgcg ctccggcctg
     8161 gccgaacttg ggccgaaact gatcgggctt gacccgacgg atctcggcac gctgaaccgc
     8221 catatggatg cggtgctccg gggccatccc tatatcaagg cgccgatcga tatcgcctgc
     8281 tgggacatcc tcggcaaggc aaccggattg ccggtctaca agcttctggg gggagcggca
     8341 caggacagga tcgccctcta ccgagcgatt tcccaggaat cgcccgaagc gatggctgcc
     8401 aagatcgccg gctacaagaa ggaaggctac accaagttcc agctcaaggt tggcggcaat
     8461 gccgataccg acattgtccg catccatgaa acgcgggcga tcctggaggc cggcgacatt
     8521 ctggttgccg atgccaacac cggctggacc cgcgccgagg ctgcgcgcat cgcggccgcc
     8581 gtcgccaatg tcgatgtcta tatcgagcaa ccctgcccga cctacgagga atgcctgtcg
     8641 gttcgccgcc gcacggtgcg ccccttcgtt ctcgacgagg tgatcggtga tctcggcacc
     8701 ctgatgacgg cgcttgccga tgatgcgatg gacatcatca acctcaagat cagcaaggtt
     8761 ggggggctta ccaaggcccg gctgttgcgc gacatttgcg ttgccaccgg cacgccgatg
     8821 actatcgagg acacctgggg cggtgatatc gtcaccgcca caatcgcgca tctggccata
     8881 tcgacgccgg aggaattctg cttctcggcg acggatttca acagctatgg cactgtcgat
     8941 atcgccgatg gagcgcccat gcgcgtcaat ggattcatga ccgcctccga tacgcccggt
     9001 cttggcgtgt ccccccgtct tgatgtgctc ggcaagccgt ccggcatgat ccgctagggc
     9061 aggcggttgg gccgcgaaca ggaaatcgtg atgacagtgg cggaggcaga agccttcgcc
     9121 atcaatatcc tatcggccaa cgggctggtg gagcatcagg ccgggccgat tgcccgcgcc
     9181 atggctctgg cagaagcagc cgaagtgcgc tcgcacggcc tttaccggtt gatcggctat
     9241 gttggcagca tccgcagcgg gcgggtcaac ccatccgcgc gtccgcagct gcaaaccatc
     9301 gccggcagct ttctgcgcgt cgatggcgac aatggctttg cccctgccgc gcatgggatc
     9361 ggcgctccgg ctctgatcga agcagcccga accaatggca tcgcggcgct ggccctgacc
     9421 aattgctatc atttttctgc cctgtggcat gatctcgatc cgctcgtgaa tgccgggctg
     9481 gcctgctggg ccttcacgct cggccaatgc atcgtggcgc ctcatggcgg cacgcgccgg
     9541 ctcatgggca ccaatccgat cgcctttggc tggccaaggg agggggagga cccgttcctc
     9601 ttcgattttg ccaccagcgt cgttgcgcgc ggcgagatcg aactgatgcg ccgggcggga
     9661 aaggacctgc ccgaaggttg ggggctcgac gccgcaggaa tgccaacacg cgatccgcaa
     9721 gccgccttgg ccggagcatt gctgccattc ggcggttaca aggggtcggc cttgtcgatg
     9781 atggtggaat tgctcgccgg cccgctcatc ggcgagccaa ccagcctcga ggcggccgcg
     9841 ctggacaatg gcgacggcgg ccctccgctg ggcggcgaac tgctgatcgc gatgagcccg
     9901 gatctcttca accgggggct ggccaacgac tggcgacagc aggcggagat gtttttcgcc
     9961 gaagcacgca agcagccagg cctcaggctg ccgtcagacc gacgccatgg cgcccttcgc
    10021 cacaatgctg aggccggcat caggatttcg aaggccctgc tggaggagtt gacgaatctt
    10081 gcctcgccgg gattgcaata ttattaaaat ttagtattat ttactttaga tggtacgact
    10141 gcgagattgg tcatggtgta tgacatcgct tggaattcgg ccgggacaac gaccctcagc
    10201 ccattgcgag acatgagagc cgagcacgca tttgcgagcg cggtgtctgt cagcaatgcc
    10261 gcatctgtca aggaaccgga cccgggaaag ccgaccaaaa ccctgagcca atatgccgat
    10321 ggcgtgatcg attggtggag cggggttctg aaaggggact tcgaagagaa cccgacaatt
    10381 ggccagacgg tcacccgcag cctgctgacg atgatcccgt tcgtcgatca gggaggcgac
    10441 atccaggatc tgaccgccaa cctctacaag tgctaccaga agggacggat tgaagcgagc
    10501 gacgggcttg ccattgccct gacggcggtt ggcgcgattc ccgaactcgg ttccgccctc
    10561 aagggtagct tcaagatcat tctgcaggcg tcgaaagagc tggctgccaa caaggcgggc
    10621 gatgcagtcg ccctggccag aaagctgttc gccatgccga tggtgcgcaa cagcatcgat
    10681 cggtttctgg cgaacgccca ggatctgcgc aaggacatga tagccggcat caacactgcg
    10741 ctggaccaga tcgccaaact caaccccgat ctggctggct atgtcgcgcg caagcgcgcg
    10801 agcctcgttg aggcggcagc cgaagggttt gatgcgctgc gcgatacgct cgctgacacg
    10861 aaaaagcgct tgatggcgcc cattcggcaa acgccggcag ccccggccgg ataccatgcg
    10921 ccgggcccga cgcctcccgg caacctgccg agtttcacgc cgcagaaata tttcaccggc
    10981 gcgatcaaac ccgcgatccg cgattatccc tggggcaagg ccgtccacat cgatgccttc
    11041 ccggcgaacc tgagcatcca ggatcgcgcg ctttacatcc gggatgcgat gaatgtcgcc
    11101 ggcattgatc aggtcgttct gcccaaaggg ggagacaagg gccagatcat ggcggccctg
    11161 caactgatgg aaggccgcga attcctctac gcccgcgcgc tcgacggcgc ccattccgcc
    11221 gaacgggtga tcgtacgcgg caacgactac agcgtgaggc ttccctcttt tgatctggtg
    11281 ccgattgccc atacgcatcc gccgtccagc ctcgaaaaat ggtttggaga atttatcagt
    11341 gtgatgccct ccgacaagga tatcgaagtg ctgcgcgaat ccggaggcta tgtgtccagc
    11401 acgattatca gcaccgccgg acaggccagg cgccacttcg acttccgcca gattctggca
    11461 aacccgcgca tgaaagcgtc gctggccaaa ctctcaatgt ctgggcgtca ggcaatgcgc
    11521 cagcatattg tcgagcaaaa attggcctat gaggaaatga tctttcagtt tggcagagca
    11581 tctgatctcg ttggtccaaa acacaagaga tatagtgaat ttcttacaca catgacgtca
    11641 ttcaatgagc ttataaaaga ctgtaatgaa atggagaaat tgataaatca actacctaga
    11701 acggtccgct gaacaggcca aggtgattga aagatccatt cgagcatgct gcgattaagt
    11761 ggacgccggt taatcgcaga agacatgctc taataccagt ggccctcaca aatgacctgt
    11821 cggtcatttc ccgggccgtc cctcgca
//
LOCUS       DAOMXG010000069        11846 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_2923_length_11846_cov_10.510304, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000069 DAOMXG010000000
VERSION     DAOMXG010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 11846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11846
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2923_length_11846_cov_10.510304"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..667
                     /gene="recR"
                     /locus_tag="PLQ11_06340"
     CDS             65..667
                     /gene="recR"
                     /locus_tag="PLQ11_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011005441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HRK24555.1"
                     /translation="MSSIAGPETERLIQLLARVPGLGPRSARRAALQLIRRREDLLAP
                     LAAAMQVAAERIRTCSQCGSIDTADPCTLCRDPRRDDRILIVVETVADLWALERARVI
                     NARYHVLGGVLSPLDGVTPKDLAIAPLLDRVTGNRVEEVILALNATVDGQTTAHYLTD
                     LLAPSGVRITRLAHGVPVGGELDYLDEGTLAAALRSRTAL"
     gene            940..3399
                     /locus_tag="PLQ11_06345"
     CDS             940..3399
                     /locus_tag="PLQ11_06345"
                     /inference="COORDINATES: protein motif:HMM:NF024267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HRK24556.1"
                     /translation="MGAASTVLGVLSLGLGPAAATDEVGLRLVTSVLPTRFDAIRFDL
                     NGPMGMLLIGLSIFSATIAILHVRARRLWNEEYEKHRATIADLQIRAERAGLFMGSEP
                     QVFVCWDGPGGQPEFEGDASILGQSIVPRRVLSFEDWLSEADRPRLVEAIDRLRATGQ
                     RFSLQVRSQSGAVMEADGQPVSGRAVMRLRLVTGVCQDLVRVSGELATQERFVSSIRL
                     MLDAIPQPVWLRHSDGRLAFVNAAYVTAVEGRDRDDVLSRGLDLMESATREAINASRS
                     SQSAFRGTAQVVVAGDRRSMDIVDVLAADGSSGGIAVDVTDLESAKRDFKQLTAAHVR
                     TLDQLSTAVALFDRHQRLAYFNRAFNDLWQLDDRFLANRPTDMEILDRLRADGKLPEQ
                     SDFRAWKQGLQQSYTSNEMLEDIWLLPSGRSLRVVTSPSTAGGVTYLFDDITEQMSLK
                     QNYNRLASMQGETLDALAEGVAVFGTNGRLQFTNPAFAVLWGIQPDLLAQSPHIDEVA
                     RLCPRETQGTWTEIRSIVCGLPDERINRAFEARNSNGKVLQLLLTPLPEGASLLTVED
                     VTSTVNAARMLSERNQALESASRFKSEFIHNVSFELRMPLTSVVGIAQLLATGTAGPL
                     NERQRSYANDLMRATDSVLALINDILDLASIDSNRLDLKIARLDLRTSIEEASTGLKD
                     RLGGAGVNLRLSIAPDIGVLLGDERRVRQILFNLMANAIACSDPGDTVLLAAERTGDR
                     IILRVVDRGPANGAGHPGPGDEAQRRIERGQSMRLSIVRSLVEMHHGQLNVEAMPDGL
                     RQVVCSMPAAAALGGSAKAAG"
     gene            3403..4974
                     /gene="tsaE"
                     /locus_tag="PLQ11_06350"
     CDS             3403..4974
                     /gene="tsaE"
                     /locus_tag="PLQ11_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HRK24557.1"
                     /translation="MAGAERTGIAAVPAQATEHSWRLSLPSLAATEALARSLAEILQP
                     GDLVTLGGGLGVGKTSFARALIRHLCESPDLDVPSPTFTLIQTYDGGRGPIVHADLYR
                     IERPEEMETLGWNEIDETAILLVEWAERAGTDLSDDRLDIRFVLDGPEGERRRARLTG
                     HGSFAPRLALMHAVHEAQAACGWTDVTRTHIQGDASSRSYERLTRGDGSASAILMIAP
                     KRPDGPPVRGGRPYSQIVKLAEDVRPFVALARGLADRGFSAPEIYAADLAAGVLVLED
                     FGREGIAVDGAPVAERYLESVSMLARLHAMDLPETLPVAEGSSHTIPRYDLEALLMET
                     ELLPDWYLPHVGQHALAMSARVGFSDLWAKALTPILTEPVTWTLRDVHSPNLMWLADR
                     QGVRRVGLLDFQDCVLGPPAYDVVSLAQDARLDVPAELELKLVGYYVRQRKEADASFD
                     NGAFARAYAVTGAQRATKILGIFARLNHRDGKPHYLKHLPRIARNLSRSLAHPALSDL
                     KIWYETHVPGLITPD"
     gene            4993..5718
                     /locus_tag="PLQ11_06355"
     CDS             4993..5718
                     /locus_tag="PLQ11_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRK24558.1"
                     /translation="MNADPLSNAAMILAAGLGTRMRPLTDSRPKPLVEVAGRTLIDYN
                     LAKLAGAGIRRAVVNVHYFADMLEAHVRTWREPVVVVSDERDRILNSGGGVKKALALL
                     GQGPFFSLNADTIWLDGPVSNLKRMAAAFDPYRMDILLLLAPTVSTIGWGNRGDFVMD
                     QAGRIRWPAPGEVAPFAYCGAAILQPQLFADTPEEFSLVTLFRRAAERDRLFGLRLDG
                     VFMHVGTPEAVIEAERALSESQL"
     gene            5715..8891
                     /gene="addB"
                     /locus_tag="PLQ11_06360"
     CDS             5715..8891
                     /gene="addB"
                     /locus_tag="PLQ11_06360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break repair protein AddB"
                     /protein_id="HRK24559.1"
                     /translation="MSIVAPRRPVPRVATIPVGQPFLAAFIEAFASGRLFGRPSPLTP
                     GEFAAAEVFVPTRRAGDALLSAFASQAEGGAVLLPRIIPLGDEDALDASLERRLAHHA
                     GGPVAVAALDRRLALFGLIERWRDAVAGLRGPAGDREPFYVASSRTDAFALAGDLARL
                     IDEMIIEDVPLDRLAAAMPASYDPERHDDYWQLTQTFLSIAAQAWPETLAEWQQRDAA
                     TITRDSLRRLAASLAEAPPPTPMVVAGSTGSVAATADLMAAIARLPMGAVVLPGLDLT
                     LDDATWSRIGHMAADLPTRYAHPQALLKRTLDRIGLQRSEVHRLGLARETPAERLASE
                     VFRPAEATALWQTRALSEADLADAKARLCLIEAADSREEALAIALVLRETLETPGKTA
                     ALITPDRQLTQAVIAELQRWGIAATESAGVPLAGTPIAGLARLALQVAESGAARDLLA
                     LLRHPLCRLGAPDADRAAVVSAVEIAGLRGQRFGRAVLERLPMVLVEAQKKHPGRRDP
                     APRRRLPPDRLAAAETHARRLSAALDGFLATFAGSSPSLAGAAAAHRALCEALLEGDG
                     AADDWSERDDGRALAAVFDQLTGSQLDLSDLALADYIGLFEALLAEETIRTWQPPHPR
                     LKIWGLLESRLIDADRILLAGLNEGVFPPEARADAFLNRSMRIALGLQPQERRIGQSA
                     HDFMMGLGGREVFLCRAARRDGAPTVASRFLRRLTAYLGAKTSAALLHKGHRYIALAR
                     ALDLPDQVVAAERPAPKVPPDLFPDELNLTEIETLYRDPYRIFARRVLGLDELDPIEP
                     PLDARDPGIAVHQALADYSRIAGELAPDRREEALLCAGEKAFAGMAKEAPDTFAFWWR
                     QFSGFAPWFVRWDTERRSALIRLHVECSGRFTLDLTGGRRLTLIGRADRIEERSDLFD
                     QPELAVIDYKTGTVPGNKEVLAGLAPQLPLTAALAMRGAFSGVARPAQMPSVSLCHVA
                     VARTDGQGKQHDVKPTHETLAEVSEAQLARLTQHLDQYLKGEIGFVSRRIPRKRDYTS
                     GYDHLARHLEWSLAGEGEDVSDEAEGET"
     gene            8888..>11846
                     /gene="addA"
                     /locus_tag="PLQ11_06365"
     CDS             8888..>11846
                     /gene="addA"
                     /locus_tag="PLQ11_06365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break repair helicase AddA"
                     /protein_id="HRK24560.1"
                     /translation="MSGAGPSRKVQLSETEQRNRACAIDPGASVFLAANAGSGKTTIL
                     TQRVIRLLLDGTPPERILCVTYTRAAAAEMQNRIFTELADWVRLDEAELAHRIADLTG
                     APPDTDCRARARQLFARAVETPGGLKLQTIHAFAERLLHLFPFEANVPARFAVMDDFA
                     GAEISATARRDSLAAGLASPDSEHGRALADLINLMGENALSELIAAGFPLIRRARLQP
                     ATTEALAIRVKRLLGLGASETPSGLAAAFLEQGLARHLWPKAESSVGARASTPTDRAF
                     VTMLHEAAAKPDDVAAVDRLADLFVTGKGTLRDKIVTKAIAEALPWLAEAIGQTKARL
                     PAYLDRQRAFAVARWSAALMQVLADILSRFQAAKRRAALVDFDDIIERTRFLVAGHGS
                     AWVLRKLDGGIDHLLIDEAQDTTPAMWQIAEALTAEFFAGDGARSLRRTVFAVGDEKQ
                     SIYSFQGAEPKEFYNSRIKLQNAAEAAGLPFHALPLTSSFRTVADVLDAVDRVFAMPA
                     HFDGLTALPEPTLHETVRKQEPGLVEIWPLEHVASSRPEDPWQEIDALGARSSPAVLA
                     ERLAGHIATLCAGERFADDGRRIRPGDVLVLVQRRDAFVGALIRALKRKGLPVAGADR
                     MRLTEQAAVLDLIAAARATLLPEDDLTLATALKSPLIGLTDDDLISLCAGRPGSLVEA
                     LEMSGEARFTEAFAILTDWRRLASSETPFGFFSAILTPMGGRLKLMSRLGPEASEGID
                     LFLEQVLARQSRAPASLAGEIEAFERLTTDVKREQDQAGGAVRILTVHGAKGLEARIV
                     YLADPQRLPVSQKDKPVFLVPDGPDDTGLLLWSRALKSAPGLVRTLAEKRRQERMQEY
                     RRQLYVAMTRARDRLYVAGWGDPAKLKSDSWYAMIQTGLKPAGILIREDDGREIVRFV
                     SVTTPPAAPAEEPEPSRQDAEQRPDWVDRPALPETVPAPPLKPSRLLAAIDQVDTPER
                     RAARERGDLVHALF"
BASE COUNT         1891 a         3828 c         3878 g         2249 t
ORIGIN      
        1 aaggccctga ccgggggcct gccgctgccg cccggcctca agctgttctg agccgctcca
       61 gcgcatgtcg tccattgccg ggcccgagac cgaacgactt atccagctgc tggcgcgcgt
      121 tccggggctt ggcccgcgct cggcccgccg ggcggcgctg caactcatcc gccgcaggga
      181 ggatctgctc gccccgctgg ccgcagccat gcaggttgcc gccgagcgca tccgcacctg
      241 ttcgcaatgc ggcagcatcg ataccgctga cccctgcacc ctctgccgcg atccgaggcg
      301 ggatgatcgc atcctgattg tggttgagac ggttgccgat ctctgggctc tggagcgggc
      361 ccgggtgatc aatgcacgct atcacgtact tggcggagtt ctgtcaccgc ttgacggggt
      421 caccccaaag gacctggcga tcgccccgct ccttgatcgg gtgacgggca accgtgtcga
      481 ggaggtgatc ctcgcgctca acgcaactgt tgatggccag accacagccc attatctcac
      541 cgatcttctg gcaccttccg gggttcggat cacccggctc gctcatggcg tgccggtggg
      601 cggcgaactc gattatctcg acgagggcac attggccgct gcgttgcgga gtcgcacggc
      661 actttgatgg tctcaccgat ctggtagggc ctctgccggg atacgcacag gtctgcccaa
      721 tcctgacaga aatcgttgcg acccctgccc gagcgtcggc gaccgggtgg caaaacgatg
      781 atgcagaaag ttaatgtgac aattgcagcg cgcgattaaa gtggcagtga ttcgaagcgg
      841 cacgaccatt ttccaacggc gcgggagagg ttcaatcgca tggctggcgc agctggagca
      901 tcggcgcaag tccctgcgcg gacgctgagg tgccgtctca tgggtgcggc ctcgaccgtg
      961 ctcggcgtcc tttcgctcgg gcttgggccg gctgcggcga ccgacgaggt agggctgcgc
     1021 ctggttacgt ccgttcttcc cacgcggttt gatgccatcc gctttgatct caacggtccg
     1081 atgggtatgc tgctgatcgg cttgtcgatc ttctcggcca ccatcgccat cctgcatgtg
     1141 cgggcacgcc ggctgtggaa cgaggaatac gaaaagcacc gcgcgacaat tgccgatctg
     1201 cagatccggg ctgaacgcgc gggcctgttc atgggttcgg aaccacaggt gttcgtctgc
     1261 tgggacggtc ctggaggcca gccggaattc gaaggcgatg ccagcatcct tggccaaagc
     1321 atcgtcccta ggcgcgtcct gagtttcgag gattggctca gcgaggcgga tcgcccccgt
     1381 ctggtcgagg cgattgaccg gttgcgcgcc acgggccagc ggttttcgct gcaggtccga
     1441 tcgcaatccg gcgcggtgat ggaggctgat ggacagccgg tgtcggggcg cgccgtgatg
     1501 cggctgcgtc tggtcaccgg cgtctgtcag gatctggtcc gggtcagtgg cgagctggcg
     1561 acgcaggagc gctttgtatc ctcgatccgg ttgatgctgg atgccattcc ccagcctgtc
     1621 tggctgcgcc acagcgatgg ccggcttgct tttgtcaatg cagcctatgt gacggccgtc
     1681 gagggccgcg accgcgacga cgtcctgtcc agagggcttg atctgatgga aagcgcgacg
     1741 cgggaggcga tcaatgccag ccgttcgtcc caatcggctt tccgggggac ggcccaggtg
     1801 gtggtcgcgg gcgatcgccg cagcatggac attgtcgatg tccttgctgc tgatggcagc
     1861 agcggaggca tcgcggtgga tgtcaccgac cttgagagcg ccaaacgcga tttcaagcag
     1921 ctcaccgccg cccatgtgcg cacgctcgac cagctttcga ccgccgtggc actgttcgac
     1981 cgccatcagc ggcttgccta tttcaaccgc gccttcaacg atctttggca gctcgatgat
     2041 cggtttctcg ccaaccggcc cactgacatg gagatcctcg accggctgag ggccgatggc
     2101 aagctgcccg agcaatccga ctttcgcgcc tggaagcagg ggttgcagca aagttataca
     2161 tccaatgaga tgctggagga tatctggctg ctgccctccg gccgttcgct gcgggtcgtg
     2221 accagcccct ccaccgccgg cggggtcacc tatctgttcg atgacatcac cgaacagatg
     2281 tctctcaagc agaattacaa tcgcctggct tccatgcagg gcgaaacgct ggatgcgctc
     2341 gctgaagggg ttgcggtttt cggcaccaac ggccggctgc aattcaccaa ccccgccttt
     2401 gcggttttgt gggggatcca gcccgacctg cttgcccaat ccccgcatat cgatgaagtt
     2461 gcccggcttt gcccgcgtga aacgcaaggg acctggaccg aaatccgctc gatcgtctgc
     2521 ggattgccgg acgagcggat caatcgcgcc tttgaggccc gcaattccaa cggaaaggtg
     2581 ctgcaactgc tgctgacgcc gctgccggag ggcgcctcgt tgctgaccgt cgaggatgtc
     2641 acctcgaccg tcaatgcggc acgcatgctt tccgagcgca atcaggcatt ggaatcggct
     2701 tcgcggttca agtcggagtt catccacaac gtttcgtttg aactgcgcat gccgctgacc
     2761 agtgttgtcg gcatcgccca gttgcttgcc accggaacgg cagggccgct caatgaacgc
     2821 cagcgcagct acgccaatga cctgatgcgc gcgacagatt cggttctggc tctgatcaac
     2881 gacattctcg atctggccag catcgattcc aaccggctcg atctcaagat tgcccggctt
     2941 gacctacgca cctcgatcga ggaggcctcc accggcctta aggaccggct tggcggcgcg
     3001 ggtgtcaacc tgcgcctgtc gatcgcgccg gatatcgggg ttctcctggg ggatgagcgt
     3061 cgcgtccggc agatcctgtt caatctgatg gccaacgcga ttgcctgttc cgaccccggc
     3121 gacacggtgt tgctggcggc cgaacggaca ggcgatcgca tcatccttag ggttgttgac
     3181 cgcggtcctg ccaatggcgc gggacatccc ggacccggcg acgaggccca gcgccggatt
     3241 gagcgcggac agagcatgcg cctgtcgatc gtgcgatcac tggtggaaat gcatcacggc
     3301 cagctcaatg tcgaggcgat gccggatggc ttgcgtcagg ttgtctgctc catgcctgcc
     3361 gcagcggcgc tgggcggtag cgcaaaggcg gcgggctgag ccatggctgg cgccgaacgc
     3421 actggcattg ctgcggttcc cgcacaagca accgaacatt cctggcggtt gtctctgcca
     3481 tcactggcgg caacggaggc cttggcccgc agcctggctg aaatcctgca acccggcgat
     3541 cttgtcaccc ttggcggcgg gctcggggtt ggcaagacca gctttgcccg agcgttgatc
     3601 cggcatctct gcgagagccc cgaccttgat gttcctagcc cgacattcac gttgattcag
     3661 acctatgacg ggggccgcgg tccgattgtt catgccgatc tctaccgcat cgagcgaccg
     3721 gaggagatgg agacgctcgg ctggaacgag attgatgaaa ccgcgatcct gctggttgaa
     3781 tgggccgagc gtgccggcac cgacttgtct gatgaccggc tcgatatccg ttttgtcctg
     3841 gacggaccgg aaggcgagcg ccgcagggcc aggctgaccg gccacggcag ctttgctccc
     3901 cgacttgcgc tgatgcacgc tgtgcacgag gcgcaggcag cctgcggctg gaccgacgtc
     3961 acgaggacgc atatccaggg cgatgcttcc agccgctcct atgagcgctt gacccgtggc
     4021 gatggttcgg ccagcgcgat cctgatgatt gcccctaagc ggccggacgg ccccccggtg
     4081 cgcggcggcc ggccctacag ccagatcgtc aagctggccg aggatgtgcg tcctttcgtc
     4141 gcattggccc gcggcctggc tgaccgcggg ttcagcgcac cggaaattta tgcagccgat
     4201 cttgccgccg gggtgttggt gcttgaggat ttcggccgcg aaggcatcgc tgtggacggg
     4261 gctccggtcg ccgagcgtta tctggaatcg gtttccatgc tggcccggct gcacgctatg
     4321 gacctgcccg aaaccctgcc ggttgccgag ggcagcagcc acaccatccc gcgctatgat
     4381 ctggaagcgc tgctgatgga aaccgaactc ttgcctgact ggtatctgcc gcacgtcggg
     4441 cagcatgcgc tggccatgag cgcgcgggtc ggtttttccg atctctgggc caaggccctg
     4501 accccgattc tgaccgagcc ggtcacctgg acccttcgcg atgtgcattc ccccaacctg
     4561 atgtggctgg ctgatcggca gggtgtccgg cgtgtcggcc tgctcgattt tcaggattgt
     4621 gtgcttggtc ccccggccta cgatgtcgtc tcgctggcgc aggatgcacg gctcgatgtt
     4681 ccggcggagc tcgaactcaa gctggtcggt tattatgtcc ggcagcgaaa agaggccgat
     4741 gccagcttcg ataacggcgc cttcgcccgg gcctatgcgg tgacgggcgc ccagcgcgcc
     4801 accaagatcc tcggcatctt tgcccgtctc aatcatcgcg acggcaagcc gcactatctc
     4861 aagcacctgc cgcgcatcgc ccgcaatctg tcgcgttccc tggcccaccc ggccctgagc
     4921 gatctcaaga tctggtatga gacccatgtt cccggcctca tcacaccgga ttaggaaagc
     4981 gggtggcatc gcatgaatgc cgatcccctc agcaatgcgg ccatgattct ggcagccggg
     5041 cttggcaccc gcatgcgccc gctgaccgac agccggccaa agccgctggt tgaggtcgcc
     5101 ggtcggacgc tgatcgatta caatctggcc aagctcgccg gggccggaat acgccgtgcc
     5161 gttgtcaacg tgcactactt tgccgatatg ctggaagccc atgtcaggac atggcgtgag
     5221 ccggttgttg tcgtctctga cgagcgcgac cgcatcctga attccggtgg tggcgtgaag
     5281 aaggctcttg cgctgctggg gcagggcccg tttttctcgc tcaatgccga tacgatctgg
     5341 ctggatggtc cggtctcgaa cctgaagcgc atggcggcag ccttcgatcc ttacaggatg
     5401 gacatcctgc ttctgctggc tcccacagtt tcaaccattg gctggggcaa tcgtggcgat
     5461 ttcgtgatgg accaggcggg gcgcatccgc tggccggcgc cgggggaggt tgctcccttc
     5521 gcctattgcg gcgcagccat cctgcaaccg cagctgtttg ccgatacgcc ggaggaattc
     5581 tcgctggtga cgctgttccg ccgcgctgcg gagcgcgatc ggctgttcgg cctgcggctc
     5641 gatggcgtgt tcatgcatgt cggcacgccc gaagcggtca tcgaggccga gcgtgccctg
     5701 tcggagagcc agctgtgagc atcgtggcgc cgcgccggcc cgtgccgcgc gttgccacaa
     5761 tcccggtcgg acaaccgttt ctggctgctt tcatcgaggc gttcgcatcc ggccggctgt
     5821 ttggccgccc gtcaccgctc actccggggg agtttgccgc tgcggaggtc tttgtgccta
     5881 cccgtcgtgc cggcgatgca ttgctgtcgg cctttgccag tcaggcagaa ggcggagccg
     5941 ttctgctgcc aaggatcatt ccgcttggcg atgaggacgc gctggatgca agcctggaaa
     6001 ggcgactggc gcaccatgca ggcgggcctg tcgccgtcgc cgcccttgat cgccggctgg
     6061 ccctgttcgg gctgatcgag cgctggcgcg atgcggttgc cggcctgcgt gggccggcgg
     6121 gcgataggga gccgttctac gttgcatcct cgcgcaccga tgctttcgcg ctcgccggcg
     6181 atcttgcccg gctgattgac gagatgatca tcgaggatgt cccgctcgac cgcctcgccg
     6241 cagcgatgcc ggcttcctat gaccctgagc gccacgacga ttactggcaa ctgacgcaga
     6301 cctttctgtc cattgccgcc caggcctggc cggagacgct ggccgaatgg cagcaacgtg
     6361 atgccgccac aatcacccgc gacagcctgc gccgcctcgc cgccagtctg gcggaagcgc
     6421 cgccaccgac gccaatggtg gtggctggtt ccaccggctc ggtcgcggcg acagccgatc
     6481 tgatggccgc catcgcccgg ctgccgatgg gcgcagtggt gctgccgggc cttgatctga
     6541 cgctggacga cgcaacctgg agcaggatcg gccatatggc cgccgatctg ccaacccgct
     6601 acgcccatcc gcaggcgctg ctcaagcgca cgctcgaccg catcggtctt cagcgctccg
     6661 aggtgcaccg gctgggtctt gcccgagaga ctcccgccga aaggctggca agcgaggtct
     6721 ttcgtcccgc cgaggcaacc gccctgtggc agacacgcgc cttgtcggag gcagacctgg
     6781 ccgatgccaa agcacggctg tgcctgatcg aggcggcgga ttcgcgcgag gaagcgctgg
     6841 ccatcgccct tgtgctgcgc gagaccctgg aaacaccagg caagaccgcc gcgctcatca
     6901 cgccggacag acaattgacc caagctgtga tcgccgagtt gcagcgctgg ggcatcgctg
     6961 cgacggaatc ggccggggtg ccgctggcgg gcacgccgat cgcgggcctc gcccgcctgg
     7021 cattgcaggt cgccgaaagc ggtgcagcga gggatctgct ggccttgctg cggcacccgc
     7081 tgtgtcgtct gggagcgccc gatgcggacc gggcagccgt ggtttcggct gtcgaaatcg
     7141 ccggtctgcg cggccagcgg ttcgggcgtg ccgtgcttga acgcctgcca atggtcctgg
     7201 tggaggcaca gaagaagcat cctggtcgcc gcgatcccgc tccgcgtcgg cgtctgccgc
     7261 ccgaccgtct ggctgcggca gaaacgcatg cccgccggct gtctgctgcc ctcgatgggt
     7321 ttcttgcgac ttttgccggg agttcaccga gcctggccgg ggccgccgcc gcccaccgcg
     7381 ccctgtgcga ggccctgctg gagggcgatg gcgcggccga cgactggagc gaacgcgacg
     7441 acggcagggc gctggccgcc gtgttcgatc aactgaccgg cagccagctt gacctttccg
     7501 atctcgccct tgccgactac atcggcctgt tcgaggcgct gctggccgaa gagaccatcc
     7561 gcacctggca accgccgcat ccccggctca agatctgggg cctgctcgaa tcgcgcctga
     7621 tcgacgccga tcgcattctg ctggccggcc tcaacgaagg ggtctttccg cccgaggccc
     7681 gggccgatgc ctttctcaac cggtcgatgc ggattgccct tggactgcaa ccgcaggagc
     7741 gccgcatcgg ccagagcgcc catgatttca tgatggggct cggagggcgg gaggtgttct
     7801 tgtgccgtgc tgcgcggcgg gatggtgcgc cgacggtcgc ctcgcgcttt ctgcgccggc
     7861 tgaccgccta tctcggcgcc aagacaagcg cggctctatt gcacaagggg caccgctata
     7921 tcgcccttgc ccgcgcactt gatctgcctg atcaggtggt tgcggccgag cggccggcgc
     7981 caaaggttcc gccggatctg ttcccggacg aactcaacct gaccgagatc gaaacgctct
     8041 atcgcgatcc ctatcgaatc ttcgcgcgcc gcgtgctcgg gcttgatgaa ctcgatccga
     8101 tcgagccgcc gcttgatgca cgcgacccgg gcattgctgt ccaccaggct ctcgccgact
     8161 attccaggat tgccggcgaa ctggcaccgg accgacgtga ggaagcgctg ctttgcgcag
     8221 gcgagaaggc cttcgccggc atggctaagg aggcgccgga cacttttgcc ttctggtggc
     8281 gacagttttc cggctttgcc ccctggtttg tccgatggga cactgagcgg cgatcggcgc
     8341 tcatccggct gcatgtcgaa tgcagcggcc gcttcacgct ggatctgacg ggcgggcggc
     8401 ggctgaccct gatcggccgg gcggaccgga tcgaggaacg gtctgatcta ttcgatcaac
     8461 cggagctggc ggtgatcgac tacaagaccg gaacggtccc cggcaacaag gaggttctgg
     8521 ccggactggc accgcaactg cctctgaccg cagcattggc gatgcgaggc gctttctccg
     8581 gcgtcgcacg accggcacag atgccttcag tcagcctttg tcacgtagcg gtggcccgaa
     8641 cggacggcca gggcaagcag catgatgtca agccgacgca tgagacgttg gccgaggtgt
     8701 ccgaggcgca gcttgccagg ctgacccagc atcttgatca atatctcaag ggcgagatcg
     8761 gctttgtcag ccgccgcatt ccgcgcaaac gggattatac ctcggggtat gaccatctcg
     8821 cccgccatct cgaatggtcg ctggccggcg aaggcgagga tgtgtcggac gaggcggagg
     8881 gcgagacatg agcggcgccg gcccctcccg caaggttcag ctttccgaga cagagcagcg
     8941 caaccgtgcc tgcgccatcg accccggcgc ttcggttttt ctggcggcca atgccggttc
     9001 tggcaagacc accatcctga cccagcgggt catcaggctg ctgctcgacg gcacgccacc
     9061 cgaacgcatc ctgtgcgtga cctatacccg cgcggcggca gccgagatgc agaaccgcat
     9121 tttcactgag cttgccgatt gggtccgcct tgatgaggcg gagcttgcgc atcgtatcgc
     9181 ggacttgacc ggcgctccgc ccgatacgga ttgccgcgcc cgggcccggc agctctttgc
     9241 ccgggcggtg gaaacgccgg gcggtttgaa actgcaaacc atccatgcct ttgccgaacg
     9301 gctgctgcat ctgtttccgt tcgaagccaa tgtgccggcg cgttttgcgg tgatggatga
     9361 tttcgccggc gccgaaatca gcgctacggc ccggcgggac agtctggccg ccggtcttgc
     9421 cagcccggac agcgagcatg gccgtgcgct cgcagacctc atcaacctga tgggcgagaa
     9481 tgcgctgtcc gagttgattg ccgccggctt tccgctgatc cgccgggctc gcttgcagcc
     9541 ggcaacgact gaggcgcttg ccatacgggt gaaacgcctg ttggggcttg gtgcgagcga
     9601 gaccccgtcc gggctcgcgg ccgcctttct tgaacagggt cttgcccgcc atctgtggcc
     9661 gaaggcggaa tcctcggttg gcgcacgcgc cagcacgccc accgatcgtg cttttgtgac
     9721 gatgctccat gaagcggcgg ccaagccgga cgatgtggct gctgtcgaca ggctggccga
     9781 tctgttcgtg acaggaaaag gcacgctgcg cgacaagata gtgaccaagg ccattgccga
     9841 ggcgctgcct tggcttgccg aggcgatcgg gcagacgaag gcccgtttgc cggcctatct
     9901 cgaccggcaa cgcgcctttg cagtcgcgcg atggtcagcc gccctgatgc aggttctggc
     9961 tgacatcctc agccgttttc aggcggccaa gcgtcgagcg gcgttggttg atttcgatga
    10021 catcatcgag cgcacacgtt ttctggtggc cggtcatggc tcggcctggg ttctgcgcaa
    10081 gctcgatggc ggcatcgacc atctgctgat cgacgaggcg caggatacga cgccggcgat
    10141 gtggcagatc gccgaggctc tgacggctga gttctttgcc ggagacggcg cccggtcctt
    10201 gcgccgcacg gtctttgcag tgggcgatga gaagcaatcg atctattcgt ttcagggtgc
    10261 cgagccgaag gaattctaca acagtcgcat caagctgcag aatgccgcag aggcggcagg
    10321 cctgcctttc catgccctgc cgctgacgtc ctcgttccgc accgtggccg atgtgcttga
    10381 tgccgtcgac cgggtgtttg ccatgcccgc ccatttcgac gggctgaccg cgctccccga
    10441 gccgaccttg cacgaaaccg ttcgcaagca ggaaccgggc cttgtcgaga tctggccgct
    10501 tgaacatgtg gccagcagca gaccggagga cccctggcag gaaatcgatg cgctcggcgc
    10561 ccgttcctcg ccggcggttc tggccgagcg gctggcaggc catatcgcca cgctctgcgc
    10621 cggcgagcgg ttcgccgacg acggacggcg cattcgtccg ggggatgtcc tggtgctggt
    10681 gcagcgacgc gatgcctttg tcggtgcgct gatccgtgcc ctcaagcgca agggcttgcc
    10741 agtggccggc gcagaccgca tgcgccttac cgagcaggcc gccgtcctcg atctgatcgc
    10801 tgccgctcgt gcgacattgc tgcctgagga cgatctgacc ctggccacgg cgctgaaatc
    10861 gccattgatc ggtttgactg atgacgacct catatcactc tgtgccgggc gcccaggcag
    10921 ccttgttgaa gcgctggaga tgtccgggga ggcgcgattc acggaggcat ttgcaatcct
    10981 gacggattgg cggaggctgg cgagcagcga gacgcccttc gggttcttct cggcaatcct
    11041 gaccccgatg ggcgggcgtc tcaagctcat gtcccggctg gggccggagg cttccgaggg
    11101 gatcgatctg tttctcgaac aggttctggc gcgccaatcc cgtgcgccgg cgtcgcttgc
    11161 cggcgagatc gaggccttcg agcggctgac gacggacgtc aagcgtgagc aggaccaggc
    11221 tggcggggcg gtgcgcatcc tcaccgtgca tggcgccaag gggcttgagg cgcgaatcgt
    11281 ctatctggcc gatccccaac gcctgccggt ctcccagaag gacaagccgg tgttcctggt
    11341 tcccgatggg ccggacgaca ccggcctgct gctctggtcc cgcgccctca agtcggctcc
    11401 cggcctggtc cgcacgcttg ccgaaaagcg gcggcaggag cgaatgcagg aatatcgccg
    11461 tcagctttat gtggcgatga cacgggcgcg tgaccggctc tatgttgccg gctggggcga
    11521 tccggccaag ctcaagtcgg acagctggta tgcgatgatt caaaccgggc ttaagccagc
    11581 cggcatcctg atacgggagg acgatggccg cgagatcgtg cggtttgtct cggtcaccac
    11641 gccaccggcc gcgcctgcag aggagccgga accgagtcgg caggacgcag aacagcgtcc
    11701 tgactgggtt gaccggccag ccttgcccga aacggtgcca gcgcctcctc tcaagccgtc
    11761 gcgcctgctc gccgccattg atcaggtcga tacgcccgaa cgtcgcgctg cccgcgagcg
    11821 gggcgatctc gtgcatgccc tgttcg
//
LOCUS       DAOMXG010000070        11541 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_3012_length_11541_cov_9.349643, whole genome shotgun sequence.
ACCESSION   DAOMXG010000070 DAOMXG010000000
VERSION     DAOMXG010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 11541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11541
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3012_length_11541_cov_9.349643"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="PLQ11_06370"
     CDS             <1..642
                     /locus_tag="PLQ11_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRK24561.1"
                     /translation="IAAARTVGGLEELDDAIKTAGGSATLVPIDLKDLEAVDRFAPVI
                     DRRWGRLDILVGNAGVLGTISPVAHLDAKVFDEVFRVNVTANFRLLKSLDPLLRLSDA
                     GRVIMLSSGAARRVRAFWGCYAASKAALEALVRAYAAETMNITPVRAMLVNPGPMRTA
                     MRANAMPGEDAETLPRPEALVPDLVKLASPEWHETGKLFDFPTRSVISFEQPA"
     gene            complement(872..2260)
                     /gene="der"
                     /locus_tag="PLQ11_06375"
     CDS             complement(872..2260)
                     /gene="der"
                     /locus_tag="PLQ11_06375"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HRK24562.1"
                     /translation="MHPTIVIVGRPNVGKSTLFNRLVGKKLALVDDQPGVTRDRRSGE
                     ARLIDLTFTIVDTAGFEDEPAASLAGRMRRQTEAAIAEADVILFVIDARIGVHPDDAH
                     FANLVRKSGKPVILIANKAEGRGGLAGAYDAFSLGLGDPLPFSAEHGEGIGELHEALR
                     HYIGVPADEAEDDDGSGDSEDEETSRGDKPMRIAIVGRPNAGKSTLINTLIGEDRLLT
                     GPEAGITRDSIGIDFSWRGRAIRVFDTAGLRRRARIEEKLEKLAATDAIRAVKFAEVV
                     VILLDATIPFEKQDLSIVDLVEREGRGVVIGLNKWDLIEARQGKLAELRETADRLLPQ
                     VKGCPLVPVSGLAGEGLDRLMQAIVKVEEIWSRRVSTSSLNRWLESAIAAHAPPAVSG
                     RRIKIRYMTQPKSRPPYFALFGNQLGALPDAYKRYLVNGLRERFDLPGVPIRLSLRTS
                     DNPYAGRKKKPE"
     gene            complement(2260..2919)
                     /locus_tag="PLQ11_06380"
     CDS             complement(2260..2919)
                     /locus_tag="PLQ11_06380"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRK24563.1"
                     /translation="MSDIFREVDEEFKRDQVAQTVKKHSNLIIGLLLAVVIGVGGYRT
                     WQYFETKKAIEAGQKFEVALALASDGKADEAKAAFEAMAREAPKGYQMLARFRLASDL
                     ARTDVAGAIKAYEALAADASIDQTLRDLASVRAGVLMVDTAGYDDLKARLEPHVAQGK
                     AWRLAAREALGLSAYKAGQLDRAGGYFEQVLADPAAGQAVRQRAEMMLALVRGGAAPA
                     K"
     gene            3193..4368
                     /locus_tag="PLQ11_06385"
     CDS             3193..4368
                     /locus_tag="PLQ11_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24564.1"
                     /translation="MFRNGLVRMVLALIVLVITLGTRGQAAPNDRPNPPRIVLDGPEE
                     IVFDPKHDACDRHDVPDVPPRAYRDAAGNVRMFALHFENRALVGPSLDALKPDCTVVF
                     RGSGSADPAAYDDRSWITATWTGDGRIIHALVHHEYQAQAHPGRCAFKDYMACWFNTV
                     LAARSGDGGQSFVKAARPVVASAPFPQTVEQGRHRGFFNPSNIVSDGVWHYMLASTTG
                     WKGQDSGVCLFRTDTIADPSRWHAFDGTGFTIRHSDPYRGKTDTPPCMTLAPFPAPVG
                     SVTRHTPSGQWIAVFQASQDRQRFPVAGIYAAASRDLRTWSEPRLVMATKTLYDNACG
                     ADYLNSYPTLIDRASASRNFETTGASADLYLSRMRVEGCNHTGDRVLVRMKVQIGVE"
     gene            4459..5319
                     /locus_tag="PLQ11_06390"
     CDS             4459..5319
                     /locus_tag="PLQ11_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRK24565.1"
                     /translation="MRALAISLAVFLTVLAAAALHAGWNAMLKLRLDPLMAMVLIHAA
                     GSVIALPALFFTGLPPASTLIWLIASTILHMGYYFALAGAYRLADMGQVYPVARGSAP
                     LLTGLFSVILLGEPLTQLGAAGIGMLGLGIFVMSLRSAKDATHMDRRALLFAGLTAVT
                     ICGYTLADGSGARASGNPLAYSAALFVMDGMALTLFVLWLRGGEAMKPILGFWPQGLA
                     GGAMSCGSYTIAIWAMTVAPIPLVAAVRETSVLFGAVIAVVFLKEPLRLNRILAAVLI
                     VIGLTLIRLQ"
     gene            5331..5834
                     /locus_tag="PLQ11_06395"
     CDS             5331..5834
                     /locus_tag="PLQ11_06395"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HRK24566.1"
                     /translation="MAKPYRFNVGIALFDRQGRVFLGKSISDGPEYVLPGLEWQMPQG
                     GIDPHEDIIAAARRELAEETGIADVRLLAVTDEYWTYDFPPHEPTGHRLEAFAGQQQR
                     WVAFRFRGQDSAIDLTASGADFYPEFSEWRWASLAEAVAGVMPYKRPTYEKVAAAFAR
                     FAGVPGQ"
     gene            5831..6850
                     /gene="epmA"
                     /locus_tag="PLQ11_06400"
     CDS             5831..6850
                     /gene="epmA"
                     /locus_tag="PLQ11_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003599277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HRK24567.1"
                     /translation="MSGSSPWWHRDRHADRRPFLLARNRLAAAIRDWFGQRGFVEVDP
                     AALAISPGNETHLHAFATDMITTGGERTQRYLHTSPEFACKKLLAAGEPLLFSLGHVF
                     RNRESGPLHAPEFTMLEWYRAGADYQAIMADCTALLALAANVCGTDRALWRGRACTLT
                     TEPERLTVAAAFARHCGLDLLASDRAALATGARDLGLHVAEDDTWSDLFSRLMSTSIE
                     PHLGMGRPTILTDYPVSEAALARKKPADPRLAERFELYVAGVELANCFGELTDADEQR
                     ARFVADMDDKQRIYGERYPLDEEFLAALAQMPAASGGALGFDRLVMLTSGAPHIDLVQ
                     WVPAR"
     gene            6847..7878
                     /locus_tag="PLQ11_06405"
     CDS             6847..7878
                     /locus_tag="PLQ11_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012319693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-2,3-aminomutase-like protein"
                     /protein_id="HRK24568.1"
                     /translation="MKRPLTRIDDLIGAGLVDETRRAALETVAARYAVALTPEMADLI
                     ERGDADDPIARQFIPRADELETAPEESADPIGDAAHSPLPGLVHRYPDRVLLKLTHIC
                     PVYCRFCFRREMVGPAGDSTMTAAEIDAALAYIAAQPTIFEVIVTGGDPLILSSRRIA
                     EITKRIAAISHVRVLRWHSRVPVVRPGAITQALVRALRHTRLAVYVAVHANHAREFTP
                     AAEAALARLADGGVALLSQSVLLRGVNDNVAALSALMRAFVRNRVRPYYLHHGDLAPG
                     TAHFRVPLDEGLALVAALRGELSGLCQPSYVIDLPGGFGKVPVASGRRLAGGWEFVDR
                     QDRRHRYNS"
     gene            complement(8059..8283)
                     /locus_tag="PLQ11_06410"
     CDS             complement(8059..8283)
                     /locus_tag="PLQ11_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24569.1"
                     /translation="MLLPEGKSFYDMGMLYATGRGGEYDLVAAHCWFNIAAARGDRDA
                     AAKRSELAAEMSAMEISTAQRAAREWLKLH"
     gene            complement(8650..9345)
                     /locus_tag="PLQ11_06415"
     CDS             complement(8650..9345)
                     /locus_tag="PLQ11_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HRK24570.1"
                     /translation="MRPIDLYYWPTPNGWKISIALEEMGLAYTTHLVNISAGDQFQPD
                     FLAISPNNKMPAIVDHDGPGGRAISVFESGAILQYLGRKTGLFYPADERARVEVEQWL
                     MWQMGGFGPMLGQNHHFRHYAPEKLPYAIDRYTNETQRLYGVLDRRLKDRDYVAGDYS
                     IADMAIVGWARLWERQGQTIAEFPHVKRWLDVVLARPAVQRGLAVSAEKRGSTDPRDP
                     KVQAVLFGNKPKG"
     gene            9497..10045
                     /locus_tag="PLQ11_06420"
     CDS             9497..10045
                     /locus_tag="PLQ11_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018042492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HRK24571.1"
                     /translation="MESANFLTATIEFVRTHQEWAVPIVFLLAFFESLAFLSLVMPAT
                     AILIGVGALIGAAGIDFWSLWFAGGIGGVLGYSASYQIGVHFGERAYAVWPFRNHPEL
                     IRKSQSFFDRFGVMAVFIGHFIGPVRAVIPVVAGVHAVKRIPFEIANVLAAFIWISSV
                     LAPGFLAGSSDLASTWLMMGAR"
     gene            10063..10350
                     /locus_tag="PLQ11_06425"
     CDS             10063..10350
                     /locus_tag="PLQ11_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24572.1"
                     /translation="MVRYLAAGACLLSLFGGLAEARPMSSRMACSEVQRLVARSGAVV
                     MSFTPTTYDRVVHDRRWCLPSEGLEQIYVPARDTPHCFAGYTCREADRMDW"
     gene            10431..10802
                     /locus_tag="PLQ11_06430"
     CDS             10431..10802
                     /locus_tag="PLQ11_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24573.1"
                     /translation="MPSPRVLFPVVALMLSVPAVAQPKGYSGPVTHATTWKNTCGQVQ
                     ALVARRGFAVLTYAGSGAAAGSVLFGGDAQNRVVADQTFCLPRQSTQPIWVPTRDNTQ
                     CFAGYTCEEGSVGNSGSRGRR"
     gene            10923..>11541
                     /locus_tag="PLQ11_06435"
     CDS             10923..>11541
                     /locus_tag="PLQ11_06435"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HRK24574.1"
                     /translation="MLDGIRKAANNWLGRIVLIVIMGFLILSFAIWGIGDMLRVQGST
                     AVAVVGKTSIDAEQVRRTYSNALEDLSQRARTRITTEQARQMGLDKQVIGRLISEAAL
                     DQKARDFGLNLADEEVVRITMAEPAFKGPSGQFDRARFYDVLRQSGLSEAMFFNEQKR
                     SLLRRTIGAAVAGDMRPPNALVDAVYRYVSEERTLKSFTLAPAVLG"
BASE COUNT         1888 a         3835 c         3613 g         2205 t
ORIGIN      
        1 atcgccgccg cccgcacggt cggcggactt gaggaactcg acgatgccat caagaccgca
       61 ggtggcagcg cgacgctggt gccgatcgac ctcaaggacc tggaagcggt cgaccgcttc
      121 gccccggtca tcgaccggcg ctggggccgg ctcgacattc ttgtcggcaa tgccggcgtg
      181 ctcggcacga tctcgccggt cgcccatctc gatgccaagg tgttcgacga ggtgttccgc
      241 gtcaatgtca cggccaattt ccgcctgctg aaatcgctgg atccgctgct gcgactgtcg
      301 gatgccggcc gggtcatcat gctgtcgtcg ggcgcggcac ggcgcgtgcg ggcgttctgg
      361 ggatgctatg ccgcctcgaa ggcggcgctg gaggctctgg tgcgcgccta tgcggcggaa
      421 acgatgaaca tcacgccagt gcgggccatg ctggtcaatc ccggcccgat gcgcaccgcc
      481 atgcgcgcca acgccatgcc cggcgaggat gcggagacgc tgccccggcc ggaagctctg
      541 gttcccgatc ttgtcaaact ggcctcgccg gagtggcacg agaccgggaa actgtttgat
      601 tttccaacgc gctccgtgat ttcgttcgag cagccggcct gagccggccg aaggtgcaag
      661 gggtgagggc gcaaagaggg tgtccggacc gggcctgacg gcccttccgg aatgacagcg
      721 gcggtgcatc tccactgtca ttccggggcg agcgaaagcg agagcccgga atccatcttg
      781 gggacagtgc aattgacgaa ggcgcaaaga tggattccgg acagggcctg acggcccttc
      841 cggaatgacg gcgcgaggca agtcggtccg ttcactccgg ctttttcttg cggcccgcgt
      901 agggattgtc ggaggtgcgc agcgacaggc ggatcggcac gcccggcaga tcgaagcgtt
      961 cgcgcaggcc attgaccagg tagcgcttgt aggcatcggg cagcgcgccg agctggttgc
     1021 cgaacagcgc gaaatacggc ggccgggact tgggctgcgt catgtagcgg atcttgatgc
     1081 ggcgcccgga gacggcgggc ggcgcatgcg cggcgatcgc gctttcgagc cagcggttga
     1141 ggctggaggt ggaaacgcgg cggctccaga tctcctcgac cttgacgatt gcctgcatca
     1201 gccggtcgag cccctcgccg gcaaggcccg agaccggcac cagcggacag cccttgacct
     1261 gcggcagcag ccggtcggca gtttcacgca attcggcgag cttgccctgc cgcgcctcga
     1321 tcaggtccca tttgttgagg ccgatgacga cgccccggcc ctcgcgctcg accagatcga
     1381 cgatcgacag gtcctgcttc tcgaacggga tcgtcgcatc gagcaggatc accaccactt
     1441 cggcaaattt gacggcgcgg atcgcgtcgg tggcggcgag cttttccagc ttttcctcga
     1501 tacgggcgcg gcggcgcagt ccggcggtgt cgaacacgcg gatggcgcgg ccgcgccagg
     1561 aaaagtcgat gccgatggaa tcgcgggtga tcccggcttc cgggccggtc agcagccgat
     1621 cctcgccgat cagcgtgttg atcagcgtcg acttgccggc attcggacga ccgacgatgg
     1681 cgatgcgcat cggcttgtcg ccgcggctcg tctcctcatc ctcgctgtcg ccggagccat
     1741 catcgtcctc cgcttcgtcc gccggaacac cgatgtaatg gcgcagcgcc tcgtgcaatt
     1801 cgccgatgcc ctcgccatgc tctgcggaaa acggcagcgg atcgcccagc cccagcgaaa
     1861 acgcatcata ggcgccggca aggccgcccc gcccctccgc cttgttggcg atcaggatca
     1921 ccggcttgcc gctcttgcgc accagattgg cgaaatgcgc atcatcaggg tggacgccga
     1981 tgcgcgcgtc gatgacgaac aggatgacat cggcctcggc aatcgccgcc tcggtctggc
     2041 ggcgcatgcg tccggccagc gaggctgccg gctcgtcctc gaaaccggcg gtatcgacaa
     2101 tggtgaaggt gagatcgatc agccgcgcct cgccagaacg gcggtcgcgc gttacgcccg
     2161 gctggtcatc gacgagcgcc agcttcttgc cgaccagacg gttgaacagc gtcgacttgc
     2221 cgacattggg ccggccgaca atgacgattg tgggatgcat tactttgccg gggcagcgcc
     2281 gccgcggacc agggcgagca tcatctcggc gcgctggcga acggcctggc cggcggccgg
     2341 atcagccagc acctgctcaa aataaccgcc tgccctgtcg agctggcccg ccttgtaggc
     2401 cgacagcccc agggcttcgc gagcggccag acgccaggcc ttgccctgcg ccacatgcgg
     2461 ttccagccgg gccttcagat cgtcatagcc ggcagtgtcg accatcagga caccggcacg
     2521 cacgctcgcc agatcgcgca gcgtctggtc gatcgacgca tcggcggcca gcgcctcata
     2581 ggccttgatc gcgccggcaa catcggttcg ggccagatcg gaggcgaggc ggaaccgcgc
     2641 cagcatctga taccccttcg gcgcctcgcg ggccatcgcc tcgaacgccg ccttggcctc
     2701 atccgccttg ccatcggacg caagcgccaa cgcgacctcg aacttctgtc ccgcctcgat
     2761 cgccttcttt gtctcgaaat actgccaggt gcgatagccg ccgaccccga tcaccacagc
     2821 cagcaacagg ccgatgatga gattgctgtg cttcttgacc gtctgggcga cctgatcgcg
     2881 tttgaattcc tcgtcaactt cgcgaaagat atccgacatc gcctgtctct catggttcac
     2941 ggccgctggc gcccggcgct ccggctagat cacgctgtgt tttgatagaa tcatcaaaac
     3001 acagtaacgt gatcgctatc aatagtttag agcatgcttt ggcgattaac cggcttccac
     3061 ttaatcgcag catgctctag cacatgcggg ccgacaaggc gagcctttcg cgttgcccgg
     3121 cgcggcatca acggtttctt gaaggtcgcg gccgcattct ggggccagga tcctgccacg
     3181 ggaaacacgc tgatgttccg caacggcctc gttcgcatgg ttctggctct gatcgtgctg
     3241 gtgatcacgc tcggcacgcg cggccaggct gcgcccaatg atcgcccgaa cccgccgcgc
     3301 atcgtgctcg acgggcccga ggagatcgtg ttcgatccca agcacgatgc ctgcgaccgc
     3361 catgatgttc ccgacgtgcc gccgcgcgcc tatcgcgatg cggcgggcaa tgtccggatg
     3421 ttcgcgctgc atttcgagaa ccgggcgctg gtcggcccgt cgctcgatgc gttgaaaccg
     3481 gactgcaccg tcgtgtttcg cggcagcggc agcgccgatc ctgccgccta cgacgaccgc
     3541 agctggatca ccgccacctg gaccggcgac ggtcgcatca tccatgcgct ggtccatcac
     3601 gagtatcagg cgcaggccca tcccggccgg tgcgccttca aggattatat ggcctgctgg
     3661 ttcaataccg tgctggcggc acgatccggc gatggtggcc agagcttcgt caaggccgcc
     3721 cggccggtcg tggccagcgc gccgtttccg caaaccgtcg agcaggggcg gcaccggggc
     3781 tttttcaacc cgtccaacat cgtctccgac ggcgtatggc attacatgct cgcctcgacc
     3841 accggatgga agggacagga cagcggcgtc tgcctgttcc gcaccgatac cattgccgat
     3901 ccctcgcgct ggcatgcgtt cgacggcacc ggcttcacga tacgccacag cgatccctat
     3961 cgcggcaaga cggacacacc gccctgcatg acgctggcgc ccttccccgc cccggtcggg
     4021 tcggtaaccc gccatacccc gtccggccag tggatcgccg tgtttcaggc ctcgcaggac
     4081 aggcagcgct ttcccgtcgc cggcatctat gctgccgcct cgcgtgatct caggacatgg
     4141 agcgaaccgc ggctggtgat ggcgaccaag accctctacg acaatgcctg cggcgcggac
     4201 tatctcaatt cctacccgac gctgatcgac cgcgcctcgg cctcgcgcaa tttcgaaacc
     4261 accggcgcaa gcgccgacct ctatctttcc cgcatgcggg tggagggctg caaccatacc
     4321 ggcgaccggg ttctggtgcg catgaaggtc cagatcggcg tcgagtaatt ctcgcgctgc
     4381 acagcgttcg ccgcttgatc aaggaccggc cctgccgcag agttgagacc tgactcggga
     4441 gggcgtgctg gccctggatt gagggccctc gccatttcgc ttgccgtttt cctgaccgtt
     4501 cttgccgctg ccgcgcttca tgccggttgg aacgccatgc tcaagctgcg gctcgatccg
     4561 ctcatggcga tggtgctgat ccatgcggcc ggctccgtga ttgccttgcc ggcattgttc
     4621 tttaccgggc tgccgccggc ctcgaccctt atctggctga ttgcctcgac catcctgcat
     4681 atgggctatt attttgctct ggcgggcgcc tatcggctcg ccgacatggg gcaggtctat
     4741 ccggtggcgc gcggctcggc gccgctgctg accggattgt tctccgttat cctgctgggc
     4801 gaaccgctga cgcagcttgg tgcggccggc atcggcatgc ttggcctcgg catcttcgtc
     4861 atgtcgctgc gcagcgccaa ggatgccacc cacatggacc gacgggcgct gctgtttgcc
     4921 gggctgaccg ccgtcaccat ctgcggctac acgctcgccg atgggtcagg agcgcgggct
     4981 tccggcaatc cgctcgccta cagcgccgcg ctgtttgtca tggatggcat ggccctcacc
     5041 ctgtttgtcc tgtggctgcg aggaggcgag gcaatgaaac cgatcctcgg cttctggccg
     5101 cagggactgg ccggcggcgc catgtcctgc ggttcctaca ccatcgccat ctgggcgatg
     5161 accgtggcgc cgatcccgct ggtggccgct gtgcgcgaga cctcggtgct gttcggagcg
     5221 gtgatcgccg tggtcttcct caaggagccg ctgcgcctca atcgcattct cgccgccgtg
     5281 ctgatcgtca tcggcttgac actgatccgc ctgcaatagc atcaacgggc atggccaaac
     5341 cctatcgttt caatgtcggc atcgcgctgt ttgaccggca aggccgtgtc tttcttggca
     5401 aatcaatcag cgacgggccg gaatatgtgc tgcccggcct cgaatggcag atgccgcagg
     5461 gcgggatcga tccacacgag gacatcatcg ctgccgcccg gcgcgaactt gccgaggaaa
     5521 ccggtattgc cgacgtgcgc ctgcttgcag tgaccgatga gtattggacc tatgactttc
     5581 cgccccatga gccgaccggg cacaggctgg aagccttcgc cggccagcag cagcgctggg
     5641 ttgccttccg ctttcgggga caggacagcg ctatcgacct gacggccagc ggcgccgact
     5701 tctatcccga attttccgaa tggcgctggg ccagtctcgc cgaggccgtg gccggggtca
     5761 tgccgtacaa gcgcccgacc tatgagaagg tcgccgccgc cttcgcccgc ttcgccgggg
     5821 tgccggggca atgagcggtt cctcgccctg gtggcaccgg gatcgccatg ccgaccggcg
     5881 cccgttcctt ctcgcccgca accggctggc ggcagcgatc cgcgactggt tcggccagcg
     5941 cggatttgtc gaggtggacc cggccgccct ggccatctcg ccgggcaatg agacgcatct
     6001 gcatgccttt gccacggaca tgatcaccac cggcggcgag cgaacccagc gctatctgca
     6061 tacctcgccc gaatttgcct gcaagaagct gctggccgcc ggcgagccgc tgctgttcag
     6121 cctcgggcat gtgtttcgca accgggaatc cggaccgctg cacgcgcccg aattcaccat
     6181 gctggaatgg tatcgcgccg gcgccgacta ccaggccatc atggctgatt gcacggcgct
     6241 gctggcgctt gccgccaatg tctgcggcac cgatcgggca ctctggcgcg gtcgcgcctg
     6301 cacgctgacc acggaaccgg agcgtttgac ggtcgccgca gcattcgccc gccactgcgg
     6361 ccttgatctg ctggcctctg accgtgccgc gctggcaaca ggcgcccggg acctggggct
     6421 gcatgtcgca gaggatgaca cctggtcgga cctgttctcg cggctgatga gcacctcaat
     6481 cgaaccgcat ctcggcatgg gccggcccac gatcctgacc gattaccccg tcagcgaggc
     6541 ggcactggcg cggaaaaagc ccgccgatcc gcgtctggcc gagcggttcg aactctatgt
     6601 cgccggggtg gaactcgcca attgcttcgg cgagctgacc gatgcggatg agcagcgggc
     6661 ccgctttgtc gccgacatgg acgacaaaca gcgcatctat ggcgagcgct atccgctcga
     6721 cgaggagttt ctggccgccc ttgcgcagat gccggcggcc agcggcggcg ctctcggctt
     6781 cgaccggctg gtgatgctga ccagcggcgc gccgcatatc gacctggtgc aatgggtgcc
     6841 ggcgcgatga agcggcccct cacccgcatc gatgacctga tcggagccgg actggtggat
     6901 gaaacccggc gggccgcgct tgagaccgtc gccgcgcgct atgccgtggc gctgacgccc
     6961 gagatggcag acctcatcga acgcggcgat gccgatgatc cgattgcccg gcagttcatc
     7021 ccgcgcgccg atgaactcga aaccgcgccc gaggaaagcg ccgacccgat cggcgatgcg
     7081 gcccattccc ccctgcccgg cctggtgcat cgctatccgg accgggtgct tctcaaactg
     7141 acccatatct gtccggtcta ttgccgcttc tgcttccggc gcgaaatggt cgggccggcc
     7201 ggcgacagca cgatgaccgc cgccgagatt gacgctgccc tcgcctatat cgccgcgcaa
     7261 ccgacgattt tcgaagtgat tgtcaccggc ggcgacccgc tgatcctgtc gtcgcggcgc
     7321 atcgctgaaa tcaccaagcg tatcgccgcc atcagccatg tccgggttct gcgctggcac
     7381 agccgggtgc cggtggtcag acccggcgcc atcacccagg cgctggtgcg cgcgctcagg
     7441 catacacgtc ttgccgtcta tgtcgcggtc catgccaatc atgcacgcga gttcacgccc
     7501 gctgccgagg ccgcgctcgc acggctcgcc gacggcggtg tggcgctgct cagccagagc
     7561 gtgctgctgc gcggcgtcaa tgacaatgtt gccgccctca gtgccctgat gcgcgccttc
     7621 gtgcgcaaca gggtccggcc ctattacctg catcacggcg atctcgcgcc cggcacggcc
     7681 catttccgcg tgccgctgga tgaggggctg gcgctggttg cggcgctgcg cggcgagctg
     7741 tccggcctct gccagcccag ctatgtgatc gacctgccgg gcgggttcgg caaggtgccg
     7801 gttgcctcgg gccgacgact tgcaggcggc tgggaattcg tcgatcgcca agaccgccgg
     7861 caccgctaca attcctagaa cactttcacg cgaaccggag tccacttcgc ttgaaaatgc
     7921 tccagtttcc cgtttttcgc attttcttca cgcgaaccgg agtccacttc gcttgaaagt
     7981 gcttttaaac ggcaacgggc gaccccgaag ggccgcccgg ctcgctgcct gtttgacgcc
     8041 tcccgattct gcgatcggtc agtgcagctt cagccattcg cgcgccgccc gctgggcggt
     8101 gctgatttcc atcgccgaca tttcggcggc cagttccgaa cgcttggctg ccgcatcgcg
     8161 atcgccacgg gcagcggcaa tgttgaacca gcaatgggcc gcaacaagat catactcgcc
     8221 gccccggcct gtcgcataca acatgcccat atcgtagaag gacttacctt ccggaagcaa
     8281 cattgtcctg atcgcctcaa ccgtcatagc ccttaactcc caatcacacg ttctatacgt
     8341 agccctgact ttgcgaacgc ttgtctgtgc aggtttttct gcaccctgat tgagtatgat
     8401 cctgctccca acaattgaat cgtggcttaa gccaaagcgt tagcaagagc ttgcccaaat
     8461 cctgacaatt gctttcatct gctcggcatt aaccgttgca ggtcgatttt tgcttttcta
     8521 tcaagcgctt gcggtcaacg catcaagctt acccgaaggt attttcaaca gccgattcac
     8581 cacgtctgcg cttgctgccg gcggatcgtc atcccgacat gtggttgcgg acagcagcag
     8641 cggggtgctc taccccttcg gtttgttgcc gaacagcaca gcctgcacct tgggatccct
     8701 cgggtcggtg ctgccgcgtt tttcggcgct gaccgcaagg ccgcgctgca ccgccgggcg
     8761 ggcgagcaca acgtcgagcc agcgtttcac atgcggaaat tcggcaatag tctggccctg
     8821 ccgctcccat agcctcgccc agccgacgat cgccatatcc gcgatcgaat agtcgccggc
     8881 cacatagtcg cgatccttca gccggcggtc gagcacccca tagaggcgct gcgtctcgtt
     8941 ggtgtagcga tcaatggcgt agggcagctt ttcgggcgcg tagtggcgga aatggtggtt
     9001 ctggccgagc atcgggccga aaccgcccat ctgccacatc agccactgtt cgacctcgac
     9061 gcgcgcgcgt tcgtcggcgg gatagaacag cccggtctta cgtccgagat attgcaggat
     9121 ggccccggat tcgaacaccg aaatcgccct gccccccggt ccgtcatgat cgacgatggc
     9181 cggcatcttg ttgttgggcg agatcgccag aaagtccggc tggaactggt ccccggcgga
     9241 gatgttgacg agatgggtgg tgtaggccag ccccatttct tccagtgcaa tggagatctt
     9301 ccagccgttc ggtgtcggcc aataatagag atcgatgggg cgcatgaggg tctcctgacc
     9361 ggcagtgtcc tcatctagcc gtgcggctca tggccgacaa atcgaaaaag ctggatcggg
     9421 ccccttgcaa ggccaccaat ccttcacgat cctgtgttga ctggtttgcc agagtgcaac
     9481 cattgtcaga gtgaacatgg aatcggcgaa ttttctcacc gcaacgatcg aattcgtccg
     9541 cacccaccag gaatgggcgg ttccgatcgt attcctgctg gcctttttcg aatcgctggc
     9601 ctttctgtcg ctggtgatgc cagcaacggc gattctgatc ggcgtcggcg ccctgatcgg
     9661 tgcggctggc atcgatttct ggtcactgtg gtttgccggc gggatcggcg gcgtattggg
     9721 atattcggcc tcctaccaga tcggcgtcca tttcggcgaa cgcgcctatg ccgtctggcc
     9781 gtttcgcaat catcccgaat tgatccgcaa gtcacaatcg ttctttgacc ggttcggggt
     9841 catggcggtg ttcatcggcc atttcatcgg cccggtgcgt gcggtcatcc cggtggttgc
     9901 cggggtgcat gcggtcaagc gcattccgtt cgagattgcc aatgttctgg cggcattcat
     9961 ctggatatcg tcagtgttgg cgccggggtt ccttgccggc agctcggatc tggccagcac
    10021 ctggctgatg atgggcgccc gttaacgaga aggaattggt ccatggtccg ttatcttgcc
    10081 gctggcgcct gcctgctgtc gcttttcggc ggacttgccg aggcgcggcc gatgtcgagc
    10141 cgcatggcct gcagcgaggt gcagcgactg gtcgcccgct ccggcgccgt ggtgatgagt
    10201 tttactccca cgacctatga ccgcgtggtg catgaccggc gctggtgcct gccgagcgag
    10261 ggactggagc agatctatgt tccggcccgc gatacgccgc actgctttgc cggatacacc
    10321 tgccgcgaag ccgaccggat ggattggtag gacctgttgc cgtttttgtg gtgcaagccg
    10381 tgaaaagctg cgctaggatt tccgcctagc accaaacagg accgaagccg atgccctcac
    10441 cccgcgttct ctttcctgtc gtcgccttga tgctgtcggt gcccgccgtt gcccagccga
    10501 agggctattc cggacccgtg acgcatgcga ctacctggaa gaacacctgc ggccaggttc
    10561 aggccctggt ggcacggcgc ggctttgccg ttctgaccta tgcgggaagc ggcgcagccg
    10621 ccggatcggt gctgtttggc ggcgatgcgc agaaccgcgt cgtggccgac cagaccttct
    10681 gcctgccgcg ccagagcacg caacccatct gggtgccgac ccgcgacaac actcagtgct
    10741 ttgcgggcta cacctgcgag gaaggcagcg tcggcaacag tggcagccgc ggccggcgct
    10801 gaccggtcgc accggtccaa tcgcaaaaaa tagcgccctt tggacgcttc gcccttcccg
    10861 tcgccgcaat tttagggcat cggagcggct gaaagtttgc atcgcaaggg atcgctgccg
    10921 ccatgctcga cggaatccgc aaagctgcca acaactggct gggccgcatc gtcctgatcg
    10981 tgatcatggg cttcctgatc ctgtccttcg caatctgggg gatcggcgac atgctcaggg
    11041 tgcagggcag caccgccgtc gcggttgtcg gcaagacctc catcgatgcc gagcaggtgc
    11101 gccgcaccta ttccaacgcg ctcgaggacc tgtcgcagcg cgcccgcacc cgcatcacca
    11161 ccgagcaggc ccggcagatg gggctcgaca agcaggtgat cggccggctg atcagcgagg
    11221 cggcgcttga ccagaaggcc cgggatttcg gcctcaatct cgccgatgag gaggttgtgc
    11281 gcatcaccat ggccgagccg gcgttcaagg gcccctccgg ccagttcgac cgtgcgcggt
    11341 tctatgatgt cctgcgccag agcgggttga gcgaggcgat gttcttcaac gagcagaagc
    11401 gctcgctgct gcgccgcacc atcggcgcgg cggtcgccgg cgacatgcgc ccgcccaacg
    11461 cgctggtcga tgccgtctat cgttacgtct ccgaggaacg cacactgaaa agcttcacgc
    11521 tcgcccctgc cgtgctgggc g
//
LOCUS       DAOMXG010000071        11252 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_3107_length_11252_cov_10.049388, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000071 DAOMXG010000000
VERSION     DAOMXG010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 11252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11252
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3107_length_11252_cov_10.049388"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2279
                     /gene="hrpB"
                     /locus_tag="PLQ11_06440"
     CDS             <1..2279
                     /gene="hrpB"
                     /locus_tag="PLQ11_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018263258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HRK24575.1"
                     /translation="RGAARRMADTLGEDVGGTVGLRARLETRVSARTRIEVITEGVFT
                     RMILDDPALTGVAAVLFDEFHERSLDADLGLALALDAQAGLREDLRLVVMSATLDGAR
                     TASLLAPGGAPIIESEGRMFPVETRHLGRDPAQRIEDQMTAAILAGLRAEPGSVLAFL
                     PGQGEILRVADRLGERIRDPGIEICPLYGAMDVRGQDRAIRPADAGRRKVVLATSIAE
                     TSLTIEGVRVVVDSGLARAPRYEPDVGITRLETVRVSRAAADQRRGRAGRTEPGVCYR
                     LWDEAQTQSLPAFARPEILDADLAGLLLDLAEWGVRDPATLTWLDPPPGPALAEARSL
                     LEGLGALTNDGTITPHGRALRALPLPPRLAAMVVRGTERGQGPEAAEIAALLSERGLG
                     GTATDLTHRLDAFHRQRGGRAEDMRRLAASWAKSGGNKIAVGAPLSAAGLLALAYPER
                     VARRRGAPGQFLLANGRAAAVDPTDSLAKAPFLVVAELTGTAAQGRILTAAPLEETEA
                     LAMFTNRIITQEEVSFDPVAASLRARRIKRLDAIILASDPLPIPPGTDSAAMLADGVA
                     RLGIHRLAFSKAQKQWRDRIAFLHAAEGEDWPDVSDAGLAASVQDWLAPFVGGITALS
                     GITPDVLGDALASLLPHTLRRRLDVEAPTHFVAPTGLSHPIDYESEGAPVLAIRVQEL
                     FGLTNHPSIARGQLPLTLHLLSPAHRPIQITRDLPGFWAGSWASVRAEMRGRYPKHPW
                     PDDPARAAPTHRAKPRGT"
     gene            complement(2365..3180)
                     /locus_tag="PLQ11_06445"
     CDS             complement(2365..3180)
                     /locus_tag="PLQ11_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1194 domain-containing protein"
                     /protein_id="HRK24576.1"
                     /translation="MTAGFARVLRVLACFMTCLWACSLTTSVRASPEEVDVALVLAVD
                     VSYSMDLEEQRLQREGYIAALNSPDVQRAIRMGMVGRIAITYVEWANAFDQTIVLPWT
                     ILDGPDSTRAATDRIAATPPRRAQRTSISGALTFSHKLLQDMPYRALRRIIDVSGDGP
                     NNAGSAVERARDAVLADGITINGLPVMVKRNLAGWGDIDHLDAYYQDCVIGGPGAFVI
                     GIRSMEEFLPATRQKIIREIASLGMADISPVQQRETKSDCMIGERMVRDRWGN"
     gene            complement(3266..4117)
                     /locus_tag="PLQ11_06450"
     CDS             complement(3266..4117)
                     /locus_tag="PLQ11_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HRK24577.1"
                     /translation="MQHDPRVTPARPDLAAAHLAGKVSADRFVEGVARRAVVPLSPLK
                     RRPAFDAPLETEVLAGEAVTVYEEEEGWAWVQLADDGYVGWMSANALAAPDPAPTRRV
                     TALRTFLYPGASIKAEPLGWLSMGATVAVTRMDGAFAATPLGFIYASHLAPPTEQAVD
                     FVAVAESLIGAPYLWGGKSSLGLDCSGLVQLSLAMTGHAARRDSDMQEKELGQPLAID
                     QDLLRRGDLVFWKGHVGIMRDADTLLHANGHHMLVVSEPLAEARARILAKGAGPVTSI
                     RRLNTQA"
     gene            complement(4117..4464)
                     /locus_tag="PLQ11_06455"
     CDS             complement(4117..4464)
                     /locus_tag="PLQ11_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015319324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HRK24578.1"
                     /translation="MAASFRPVQALRLWHDVSLALVREEEIDLSQRQLAILLTIYLEM
                     PPHTVRGLAKRLNVTKPVITRALDSMGSLDLVSRRRDDLDRRNVIVQRTVRGSLFLEK
                     LGDRIARCMAEIH"
     gene            complement(4546..4800)
                     /locus_tag="PLQ11_06460"
     CDS             complement(4546..4800)
                     /locus_tag="PLQ11_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6489 family protein"
                     /protein_id="HRK24579.1"
                     /translation="MNVTINIDCTPLEARQFFGLPDVQPMQEAVLGELQRKMLAEMNN
                     FSTDKIMSAWLSMAPQNAEWMKMMFDQFSAFTGGKKPSGR"
     gene            4904..5432
                     /locus_tag="PLQ11_06465"
                     /pseudo
     CDS             4904..5432
                     /locus_tag="PLQ11_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684916.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            complement(5470..6540)
                     /locus_tag="PLQ11_06470"
     CDS             complement(5470..6540)
                     /locus_tag="PLQ11_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004671934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRK24580.1"
                     /translation="MMAREPASVEFRNVTKAYGSVVAVDDVSFSIDPATLVTLLGPSG
                     CGKTTCLRMIAGLEMATKGQIFIGKRDVTHLSAADRDVSMVFQSYALFPHMSVLDNAA
                     YGPSVQGKPKQQAHEMAMDKLRMLGLDTMANRLPSELSGGQQQRVAIARALVLEPQVL
                     LFDEPLSNLDAKLRRRVREEIRELQLRLNLTVAYVTHDQEEALAVSDKVIVMSNARIA
                     QVGTPRELYDSPASLFVADFVGDSNLLEAEVIEVGKQGARARIGGAVELTLAQSDVAQ
                     GAAKLMVRPGSVRLGRKAVNAETLKGRVLKAAYIGKHMEYTIETDAGEVFVTDDIRPD
                     AWHSGDDIYVSILKDRVKLLQH"
     gene            complement(6554..8737)
                     /locus_tag="PLQ11_06475"
     CDS             complement(6554..8737)
                     /locus_tag="PLQ11_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008123889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRK24581.1"
                     /translation="MRKKALLWGAIALAALVLTPWYIEPVPGAVAGGWLSRLINPFAS
                     ALSLGLTGRWWLLLALLPLLALIRIGEDFASARMLTIVGLGGLALVFLQGFLIGLNGW
                     TLDAFAALFGGPGPKQGGMGVGGFLALAAFLMLTCLGLAGQGWCRGDVFVVAAIGWVV
                     ALIGLFVFLPVLTILASAFQDNAGHFAPALFWQKFTDSSIWGLGCLGGGTACGVAWNT
                     VFLGVLVGLSSTALGLAFALIALRTRFAFKGSLKLLAILPIITPPFVIGLALILIFGR
                     SGAVSSLLFQWFGIPPSRWIYGLPGVFIAQTLAFTPAAYLVMLGVVQGISPTLEEAAQ
                     TLRARPWTIFATLTWPLMRPGLANSFLLGFVESLADFGNPLVLGGNFEVLSTKIFFAV
                     VGAATDQGRAAVLSLVLLGFTLAGFAVQQLWIGKKVYTTISGKGDAGLPLPLPKRVEW
                     GCYLVAIPWGVFTIAVYLLIFYASMVKTIGRDYTFTLHHYITAFNIENGPGGLLFSGS
                     AWPSFFATVKIALIAAPLTAAIGILTAYLLTRQTFAGKRAFEFGTLLSFAIPGTVVGV
                     SYILAFNVPPIEITGTALILVICFIFRNMPVGVRAGIASLSQIDKSLDEASLTLGARS
                     FTTLRQVILPLLKPAIIASLVYAFVRAMTAVSAVIFLVSAQYNMATIYIVGRVDAGEH
                     GLAIAYSATLILVMGFAIWLINTVIGERKIGRRSAAPAAVPMAAG"
     gene            complement(8784..9815)
                     /locus_tag="PLQ11_06480"
     CDS             complement(8784..9815)
                     /locus_tag="PLQ11_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRK24582.1"
                     /translation="MPAVAARLAFLALVALPLGSLSASAQSSVTLYCSILEEQCREGA
                     ALFEKATGAKVQMIRRSTGETYATIKAESANPKGDVWWGGPGEAHLQAAEEGLTEVYK
                     SPMLDQLQDWAVRHAEKSGYRTTGTYLGALGIGMNTEVLKAKNLPEPKCWADLLDPKF
                     RDEIMIADPNSSGTAYTALATIVQVFGDDKGFEVLKGLHRNISQYTKSGIGPINALKL
                     GETAVGIGFIHDMVTQKLAGAPIKAIAPCEGTGYETGSVSLIKGGKNAELGRKFVDFA
                     LGVEAQAINNKLKIYSMPSNRNVTVPDDAPDFKAMKFIAYDTGKYGSAAVRTGLLKKW
                     GDEVKSLPR"
     gene            complement(9878..10918)
                     /locus_tag="PLQ11_06485"
     CDS             complement(9878..10918)
                     /locus_tag="PLQ11_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRK24583.1"
                     /translation="MTYQTIRNLILAGTAISALGAMGGTAQAQGQLVLYCAVQEEWCR
                     AVSTAFEKETGIKVAMTRKSSGEFYAQLKAESANPRGDIWWGGTGDPHMQAAEEGLTE
                     AYTSPNMSQLHDWAVKAHNDTKGRAVGIYTGALGFGYNAKMLAAKKLPEPKCWADLAK
                     PEYKDEVQVADPNSSGTSYTMLATIVQLMGEDKAFEWLKAVHKNINQYTKSGAAPAKA
                     TSLGETTIGITFMHDMLAIQRENPDVKVVAPCEGTGYEIGSMSMVKGTRNAEIAKKFY
                     DWALTPGPQALGAENKQFQTPSNKATPMPKFGLDMATVKLINYDFGKYGSSSVRNALL
                     AKWDKDVKSAPK"
BASE COUNT         2163 a         3641 c         3515 g         1933 t
ORIGIN      
        1 cacgcggcgc ggcccggcgc atggccgata cgctcggcga ggacgtgggc ggaacagtcg
       61 gactgcgagc caggctggag acgagggtca gcgcgcgaac gcgcatcgag gtcatcaccg
      121 aaggagtgtt tacccggatg atccttgatg atccggcact gaccggtgtg gccgccgtat
      181 tgttcgacga gttccatgaa cgctcgctcg atgccgacct cggtcttgca ctggccctcg
      241 atgcacaggc aggcttgcga gaagacctca ggctcgtggt catgtccgca acactcgatg
      301 gcgcccgcac cgcgtcattg ctcgcgccgg gcggcgcgcc gatcatcgag agcgaaggcc
      361 gcatgtttcc ggttgaaacc cgccatctcg gccgcgatcc ggcccaacgc atcgaggacc
      421 agatgacggc agcgatcctt gccgggctgc gggccgagcc aggttcggtg ctcgccttcc
      481 tgcccggaca gggcgaaatc ctgcgcgtcg ccgaccgtct cggcgaacga atcagagacc
      541 ccggcatcga gatctgcccg ctctatgggg ccatggacgt ccgcggtcag gatcgcgcca
      601 tacgtccggc cgacgcgggc cggcgcaagg tggtgctggc gaccagcatc gcggaaacgt
      661 cgctgacgat cgagggcgtg cgggtggtgg tcgattcagg gctggcgcgg gcgccgcgct
      721 atgagcccga tgtgggcatt acccggctgg agacagtgcg ggtgtcgcgc gcagccgccg
      781 accagcggcg tggccgcgcc ggccgcaccg agcccggggt ctgctatcgc ctgtgggacg
      841 aggcacaaac acaaagcctg ccggcttttg cgcggccgga aatcctcgat gccgatctgg
      901 cgggcctgtt gctcgacctt gccgaatggg gcgtgcgtga tcctgcaacg ctgacctggc
      961 ttgatccgcc gcccggtccg gcgctggccg aagcgcggtc gctgcttgaa ggtctgggcg
     1021 cattgacgaa cgatggcacg atcacgccgc acggccgcgc cctgcgcgcc ttgccgctgc
     1081 cgccgcggct agcggccatg gtggtgcgcg ggacggagcg cgggcagggt ccggaggccg
     1141 ccgaaatcgc tgccctgctc agcgaacgcg gacttggtgg cacggccacc gatctgaccc
     1201 accggctcga tgcgttccac cgccagcgcg gcgggcgcgc cgaggatatg cggcgactgg
     1261 cagcaagctg ggccaaatcc ggtgggaaca agattgccgt cggcgccccc ctgtcggctg
     1321 cgggtctgct ggcgctcgcc tatccggagc gtgtggcccg gcggcgggga gcgcctgggc
     1381 agttcctgct cgccaatggc cgcgccgcag cggtggatcc caccgattct ctggccaagg
     1441 cgccgtttct cgttgtcgcg gaactgacag gcacggccgc gcaagggcgc attctgaccg
     1501 ccgcaccgct tgaagagacc gaggctctgg ccatgttcac caaccggatc atcacccagg
     1561 aggaggtgag ttttgacccc gtcgccgcca gcctgcgcgc acggcggatc aagcggctcg
     1621 atgccatcat cctcgcctcc gatccgctgc ccataccgcc ggggaccgac agcgcggcca
     1681 tgctcgccga cggggtggcg cgcctgggca ttcatcggct ggccttctcg aaagcacaga
     1741 agcagtggcg tgaccggatc gcattcctgc atgcggcgga gggcgaggac tggccggatg
     1801 tgtcggacgc cggactggct gccagcgtgc aggactggct ggcaccgttt gtcggcggga
     1861 tcaccgccct ttcgggtatc actccggatg tgctcggcga tgccctcgcc tcactgctgc
     1921 cgcacaccct caggcgtcgg ctcgacgtgg aagcgccaac ccatttcgtc gcgcctaccg
     1981 gtctcagcca ccccatcgac tatgaaagcg aaggcgctcc ggttctggcg atccgtgtcc
     2041 aggaactgtt cggcctgacc aaccatccga gcatcgcgcg gggacagctc ccgctcacgc
     2101 tgcacctgct ctcgccagcg catcggccga tccagatcac ccgcgatctg ccaggcttct
     2161 gggccggctc ctgggcgagc gtcagagcgg agatgcgggg gcgctatccc aagcatccct
     2221 ggccggatga tcctgcccgg gctgctccga cgcatcgggc caaaccacgc ggaacctgac
     2281 ggtttgccgg tgcatccgcc cgagggcgcg tgctttccgg ccggtgtcgc gatgcggcca
     2341 aaccatgtcc cgccgggacc gggatcaatt accccagcgg tcgcgcacca tgcgctcgcc
     2401 gatcatgcaa tcgctcttgg tctcgcgctg ctgaaccgga gagatatcgg ccatgccgag
     2461 gctggcaatc tcgcggatga tcttctggcg ggtcgcggga agaaactcct ccatcgagcg
     2521 gatgccgatc acgaaagcgc ccggcccgcc aatcacgcaa tcctgatagt aggcgtcgag
     2581 atgatcaatg tcgccccaac cggcaaggtt acgtttgacc atcacaggca ggccattgat
     2641 ggtaatgccg tcggccagca ccgcgtcgcg tgcccgctcc accgccgaac cggcattgtt
     2701 gggaccatca cccgatacgt cgatgatcct gcgcaaggcg cgatagggca tgtcctgaag
     2761 cagcttgtgg ctgaaggtga gcgcgccgga gatcgaggtg cgctgcgccc ggcgcggcgg
     2821 ggttgcggcg atgcgatcgg tggccgcgcg ggttgagtcc ggcccgtcca gaatggtcca
     2881 gggcaggacg atggtctgat cgaaggcgtt ggcccattcg acataggtaa tggcaatgcg
     2941 gccgaccata cccatgcgga tggcacgctg aacatccggc gaattgagcg ccgcaatgta
     3001 accctcgcgc tgcaatcgct gctcttcaag gtccatggaa taagaaacat ccacggcaag
     3061 aaccagggcg acatcgacct cttcagggct agcccggaca gacgtcgtca acgagcatgc
     3121 ccagagacag gtcatgaagc aggccaagac ccgcagcacc cgagcgaacc ctgccgtcat
     3181 cgcgcgcctc cccgtctctt ccgggatttg acctcagttt gtcagggttg caggcgctgt
     3241 ccagccgaaa atcgtggtgg atcgttcacg cctgcgtgtt gaggcgacgg atcgaagtga
     3301 ccgggcccgc acccttcgcc aggatgcgtg cccgcgcttc ggcgagcggc tcgctgacca
     3361 ccagcatatg atgcccgttg gcatgcagca gcgtgtcggc gtcgcgcatg attccgacgt
     3421 gccccttcca gaacaccaga tctccgcggc gcaacaggtc ctgatcgatc gccagcggct
     3481 ggccgagttc cttttcctgc atgtcggaat cgcgccgggc ggcatggccg gtcattgcca
     3541 gcgacaattg caccagaccg gagcagtcga gcccgaggct ggacttgccg ccccagagat
     3601 agggagcccc gatcagcgat tcagcgaccg cgacaaaatc gaccgcctgt tctgtaggcg
     3661 gtgcaagatg cgaggcatag atgaagccga gcggggttgc ggcaaacgca ccatccatgc
     3721 gcgtgaccgc aaccgtcgcg cccatcgaca gccatccgag cggctcggcc ttgatgcttg
     3781 ctcccggata gagaaacgtc cgcaatgcag tcacccgacg ggtgggggcc ggatccggag
     3841 cggcaagggc attggccgac atccagccaa catagccatc gtccgcaagc tgcacccagg
     3901 cccagccctc ttcctcctcg tagaccgtca ccgcctcacc agcgagcact tcggtttcca
     3961 acggcgcatc gaaagcgggg cgacgcttga gcggagacag cggcacgacg gcgcgacggg
     4021 cgacgccctc gacaaaacgg tcggcactga cctttccggc cagatgggcc gccgccagat
     4081 ccggacgggc cggggtcacc cgaggatcgt gttgcatcag tgaatctccg ccatgcagcg
     4141 ggcgatcctg tcaccaagct tttcaagaaa cagtgaaccg cgcaccgtgc gctgaacaat
     4201 cacattgcgt cggtccaggt cgtcgcgacg gcgcgatacc agatcgaggc tgcccatgga
     4261 atccagcgcg cgggtgatca ccggcttggt cacattgagg cgcttggcca atccccgcac
     4321 cgtatggggc ggcatttcga gataaatcgt cagaaggatc gccaactgcc gctgcgacag
     4381 gtcgatctcc tcctcccgca ccagcgccag tgacacgtca tgccacaggc gcagtgcctg
     4441 cacggggcga aacgacgccg ccataaccgc tccattgttt cggagccgtt ttaatccgga
     4501 tggctgaaag aatcaacccg cctgccagcg gatcatccgt ttgcgctacc ggcccgacgg
     4561 cttcttgccg ccggtaaagg cgctgaactg atcgaacatc atcttcatcc actcggcatt
     4621 ctgcggcgcc atcgacagcc aggccgacat gatcttgtcg gtcgagaagt tgttcatctc
     4681 cgcgagcatc ttgcgctgca attcgcccag aaccgcctcc tgcatcggct ggacatccgg
     4741 caggccgaaa aactgacggg cttccagagg cgtgcagtca atattgatgg tcacgttcat
     4801 gctgctctcc ccttgtcggc ggtgaatgct gacaactttc ccggctgaat gataacaggc
     4861 ttggatcatc ggcggcaaac gatcgttgac ggtgctggca gggttgcgaa cgctggtcgt
     4921 cgatgatgcg ttgaaggccg gaacccatat cggcccggtg gtcgatgaaa gccagctcaa
     4981 acaggacctc gactatgtcg cgattgcccg cgacgagggc gcccgcctgg tctgcggcgg
     5041 cgagatcctc aatcgcgcta cgccggggtt ctatttccag ccggcgctgt tcagcgaggt
     5101 caacaatcgc atgcggatcg cgcgcgaaga ggtgttcggc ccggtcgcag cggtcatccg
     5161 ggtcagttcc tacgaggagg cgctggcggt tgccaacgat accgacttcg gcctgtcctc
     5221 gggcatctgc accacgtcgc tgaaatatgc ggcgcatttc aagcgcaatg tggaggccgg
     5281 aatggtgatg gtcaacctgc ccactgcggg ggtcgattat catgtgccct tcggcgggcg
     5341 caaaggctcc agctatggcc cgcgccgaac agggggccta tgcgcgcgag ttctacacca
     5401 cggtcaagac agcctatacc ctggcgggat gagcattccc ggcgacggga agcggcggtc
     5461 agccgaccat caatgctgca acagcttgac gcggtccttg aggatcgaga catagatgtc
     5521 gtcgccactg tgccaggcat cgggacgaat gtcatcggtg acgaaaactt cgccagcgtc
     5581 ggtctcgatc gtatattcca tgtgcttgcc gatataggcg gccttgagaa cgcgcccctt
     5641 gagcgtttca gcattgaccg ccttgcggcc aagccggacc gaaccgggcc ggaccatcag
     5701 cttggcggcg ccctgcgcca catcgctctg ggcaagcgtc agttcaaccg ccccaccgat
     5761 acgcgcccgc gcgccctgct tgccgacctc gatcacctcg gcttcgagca gattggaatc
     5821 gccaacgaaa tcggcaacga acaggcttgc gggggaatcg taaagttcgc gcggcgtgcc
     5881 cacctgggca atccgggcat tggacatcac gatgaccttg tcggaaaccg ccagcgcctc
     5941 ctcctgatcg tgcgtgacgt aggcgacggt gagattgagc cgaagctgta gttcacggat
     6001 ctcctcgcgg acccgccggc gaagtttggc atcaagattg gacagcggct catcgaacag
     6061 cagcacctgc ggttccagca ccagggcccg cgcgatggca acacgctgct gctgaccacc
     6121 cgacaattcc gacggaaggc gattggccat ggtgtcgagg ccaagcatcc ggagcttgtc
     6181 catcgccatc tcgtgggcct gctgcttggg cttgccctgc acactcggcc cataggcggc
     6241 attgtcgagc acgctcatgt gcgggaacag cgcataggac tggaacacca tcgaaacgtc
     6301 gcggtcggcg gccgacagat gggtgacgtc ccgcttgccg atgaatatct ggcctttggt
     6361 ggccatctcg aggccggcaa tcatccgcaa acaggtggtc ttgccgcagc cggatgggcc
     6421 cagcagcgtg accagggtcg ccggatcgat ggaaaatgac acatcgtcca ccgccaccac
     6481 gctgccatag gccttggtga cgttccggaa ttcgacggaa gcaggttctc tagccatgat
     6541 atccccgtag ttctcaaccc gcagccatcg gcacggcggc aggcgccgcc gagcggcgac
     6601 cgatctttcg ttcaccgatc acggtgttga tcagccagat ggcaaagccc atgaccagga
     6661 tcaacgttgc ggaataggca attgccaatc cgtgctcgcc ggcatcgacg cggccgacga
     6721 tatagatcgt cgccatgttg tattgcgccg agaccagaaa gatcacggca ctgaccgccg
     6781 tcattgcccg cacgaaagca tagacaagac tggcaatgat cgcaggcttg agcaacggca
     6841 ggatcacctg tcggagcgtg gtgaaggatc gcgcacccag cgtcaacgag gcttcatcga
     6901 gactcttgtc gatctggctg agcgaggcaa tgcccgcccg cacgccgacc ggcatgttgc
     6961 ggaagatgaa gcagatgacg aggatcagcg ccgtgccggt aatctcgatc ggcggcacgt
     7021 tgaacgccag aatgtaggag acgccaacca cggtgccggg aatggcgaag gacagaagcg
     7081 tgccgaactc gaaggcgcgt ttgccggcaa aggtctgccg cgtcagaaga taggccgtca
     7141 ggatgccgat ggcggcggtc agcggtgcgg cgatcaacgc gatcttcacg gtggcgaaga
     7201 acgacggcca ggcactgccc gagaacagca gtccgccagg accattctcg atgttgaatg
     7261 ccgtgatgta gtgatgcagc gtgaaggtgt agtcgcggcc gatggtcttg accatgctgg
     7321 catagaaaat cagcagatag accgcgatcg taaacacacc ccatggaatg gcgacgagat
     7381 agcaccccca ttcgacgcgc ttgggcagag gcagcggcag accggcatcg cccttgccgg
     7441 aaatggtggt gtagaccttc ttgccgatcc acagctgctg cacggcaaag ccggcgagag
     7501 tgaagcccag cagcaccagt gacaagacgg ccgcccgccc ctgatcggtc gcggcgccga
     7561 ccacggcaaa gaagatcttg gtggacaaga cctcgaaatt gccgcccaga accagcggat
     7621 tgccgaaatc ggcaaggctt tcaacaaaac ccagcaggaa ggaattggcc agaccgggtc
     7681 gcatcaatgg ccaggtcagg gtggcgaaga tcgtccaggg ccgcgcgcgc agcgtctgcg
     7741 cggcctcttc cagcgtcggc gagatgccct gaaccacccc cagcatcacc agataggccg
     7801 ccggcgtaaa ggccagcgtc tgggcgatga acacgcccgg aaggccatag atccagcggc
     7861 tcggcgggat gccgaaccac tggaacagca agctggaaac cgcgccggag cgaccgaata
     7921 tcaggatcag cgcaagaccg atcacaaagg gcggcgtgat gatcggcagg atggcaagca
     7981 gcttcagact gcccttgaag gcaaatctgg tgcgcagcgc aatcagcgcg aaagccagcc
     8041 cgagcgccgt cgaggacagc ccgaccaata ccccgaggaa aaccgtgttc caggccacac
     8101 cgcaggctgt gccaccaccc agacagccaa gcccccagat cgaggaatcg gtgaatttct
     8161 gccagaacag cgcaggcgca aaatggccgg cattgtcctg aaaagcgctg gccagaatgg
     8221 tcagaaccgg gaggaaaaca aacaggccga tgagcgcgac cacccagccg atcgccgcaa
     8281 ccacaaagac atcaccccgg caccagccct gcccggccag gcccaggcag gtcagcatca
     8341 ggaatgccgc cagggccagg aagccaccaa cccccatgcc cccctgcttg gggcccggcc
     8401 cgccgaacag ggcggcaaag gcatctagcg tccagccatt gaggccgatg agaaagccct
     8461 gcaggaacac cagagcaaga ccgccaagac cgacgatcgt cagcatccgg gcactggcga
     8521 aatcctcgcc gatcctgatc agggccagca acggcagcag cgccagcagc agccaccagc
     8581 ggccggtcag gcccagcgac aaagccgatg cgaagggatt gatcagccgg ctgagccagc
     8641 cacccgccac cgcgccgggc accggctcga tataccaggg cgtcaacacc agcgctgcca
     8701 gggctatcgc gccccacagc aaagccttct tgcgcatgtc gcccctcccc ggagacgttc
     8761 ggccgcctgc ccggccttct tgctcagcga ggcaggctct tcacctcgtc gccccatttc
     8821 ttcagcaaac cggtccggac cgctgcggag ccgtatttcc cggtatcata ggcaatgaac
     8881 ttcatcgcct tgaaatccgg cgcatcgtcc ggcacggtca cgtttcggtt tgacggcatc
     8941 gaatagatct tgagcttgtt gttgatcgcc tgcgcctcga cgccgagagc gaaatcgacg
     9001 aatttccggc ccagttcggc gttcttgccg cccttgatca gcgaaaccga ccccgtctcg
     9061 tatccggtgc cttcgcaagg ggcgatcgcc ttgatcggcg ctccggccag cttctgggtc
     9121 accatatcgt ggatgaagcc gatgccgacg gcggtctcgc caagcttgag cgcattgatc
     9181 ggaccaatac cggacttggt atactggctg atattcctgt gcaggccctt gagcacctcg
     9241 aagcccttgt cgtcgccaaa tacctgcacg atcgtcgcca gcgccgtgta agccgtaccc
     9301 gaggagttcg ggtcggcgat catgatttca tcacgaaact tcggatcgag cagatcggcc
     9361 cagcatttgg gttcaggcag gttcttggcc ttgagaacct cggtgttcat gccgatgccg
     9421 agcgcgccga ggtacgtgcc ggtggttcga tagccggatt tctcggcgtg acgcaccgcc
     9481 caatcctgca actggtcgag catcggcgac ttgtagacct cggtcaggcc ttcctctgcc
     9541 gcctgcagat gcgcctcccc cggaccgccc caccagacat cgcccttggg gttggcagac
     9601 tcggccttga tggtggcata ggtctccccg gtcgacctgc ggatcatctg caccttggcg
     9661 cccgtcgcct tttcaaacag cgccgcgcct tcacggcact gctcttcaag gatcgagcaa
     9721 taaagcgtca ccgaactctg cgccgatgcc gacagggagc ccagcggcaa agcgacaaga
     9781 gccaagaacg caaggcgggc ggcaacagcg ggcatgggca tgacgattct ccgatgggcg
     9841 acgggcagca ccgccggcgc tgcccgttct gcttgactta cttcggcgcg ctcttgacgt
     9901 ccttgtccca tttggcgagc agcgcgttgc gcaccgagga cgagccgtac ttgccgaaat
     9961 cgtagttgat cagtttcacc gttgccatgt cgagcccgaa cttgggcatc ggcgtcgcct
    10021 tgttcgacgg ggtctggaac tgcttgttct cggcaccaag ggcctggggc ccgggcgtca
    10081 gcgcccagtc atagaatttc ttggcgatct cggcattgcg ggtgcccttg accatcgaca
    10141 tcgagccgat ctcatagccg gtgccctcgc agggagccac gaccttcaca tccgggttct
    10201 cacgctggat cgccagcatg tcgtgcatga aggtgatgcc gatcgtcgtc tcgccgaggc
    10261 tggtcgcctt ggccggcgcg gcgccggatt tggtatactg gttgatgttc ttgtgaactg
    10321 ccttgagcca ttcaaacgcc ttgtcctcgc ccatcagctg cacgatcgtt gccagcatgg
    10381 tgtaggaggt gccagacgag ttcggatcgg cgacctgcac ctcgtccttg tattccggct
    10441 tggcgagatc ggcccagcac ttgggttcgg gcagcttctt ggccgccagc atcttggcat
    10501 tgtagccaaa gcccagagcg ccggtataaa tgccgaccgc gcggcccttg gtatcgttat
    10561 gtgccttaac cgcccagtca tgtagctggc tcatgttcgg cgaggtatag gcctcggtaa
    10621 ggccctcttc ggccgcctgc atgtggggat cgcccgtgcc gccccaccag atgtcgccgc
    10681 gcggattggc cgattcggcc ttgagctggg catagaactc gcccgaactc ttgcgcgtca
    10741 tggcaacctt gatgcctgtc tctttttcaa acgccgtcga aacggcccgg caccactcct
    10801 cctggacagc gcagtagagg acgagctggc cctgagcctg ggcagttccg cccatcgcgc
    10861 caagcgcact tatggccgtg cccgccagaa tgaggttgcg gatggtttga tacgtcatga
    10921 agctctcccg ttatgtggcg ccgtatccga cgcttctgtg acaggttagc attgaacaat
    10981 gacgccccgt caacaaaaga cagtgatcca tcaaccgttc ggcactgcaa tggccatggg
    11041 tggtcgacag atgcggccca cgaccggtgg cgacttcagg ccaccaccgc ccgctgcgcg
    11101 aaaaatgacc gtcgctattc ctcctggaag gcttcttctc gtgtttttga gaaggagggg
    11161 agcagaacca gggtgagcag aatagcggcc agcgccagca tggtggcact gatcggccgg
    11221 gtgaccagca ccatcggatc gcccttggac aa
//
LOCUS       DAOMXG010000072        11183 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_3138_length_11183_cov_10.398454, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000072 DAOMXG010000000
VERSION     DAOMXG010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 11183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11183
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3138_length_11183_cov_10.398454"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..1506)
                     /locus_tag="PLQ11_06490"
     CDS             complement(52..1506)
                     /locus_tag="PLQ11_06490"
                     /inference="COORDINATES: protein motif:HMM:NF019351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HRK24584.1"
                     /translation="MDRRLFLAGAATVTGLAAARVPASAQSPLLTVTTRQIEVGRRGA
                     TVFGVAGANGRHGILAREGDRFSGALLNATAEPLVMHWHGQIYAPAEQDRARTGGGEL
                     APGQSDLHDFRLTPGTHWMHSHTLTEQQLLAAPMVTREKDAGDVQDVIVMLHDFSFRP
                     PAELLAGLGGVNAHSGHAGHGATPAAPAQAGGQGAHAGHGMQHGGANHSAMGHGAGQP
                     HANDIQYDAFLANDRTLEDPEVVRVERGSKLRLRIINGATATAFFIDTGAVTARCIAV
                     DGSPCQPHPGRRFPLAQGQRIDLVLDIPASGGAFPIFAQVEAERMLTGIILATAGATV
                     RKYSAKSEHVAKHTDLSLDLALRAVHPLPTRAPSRIEHIMLGEGPNYRWTINGAIHGE
                     HKPILAKVGERIEFMFMNPTTMMHPMHIHGHHFQVVGSRSGRFSGPVRDTVIVPPHTP
                     VMVAIDFDKPGSWYLHCHHLYHMATGMMTEVVVS"
     gene            complement(1635..2000)
                     /locus_tag="PLQ11_06495"
     CDS             complement(1635..2000)
                     /locus_tag="PLQ11_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020094869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24585.1"
                     /translation="MRITLTAAVFLAGSLAFAHEPRKGPNGGALVDAGKSYHIELIAT
                     GTPEVMLILSDSADKPVASTGFKASAIIVVDGKPQRFALEPTDGGRLVGRAPVAVPAG
                     SKGAVQLTAPDGTTSQAKF"
     gene            complement(2017..5160)
                     /locus_tag="PLQ11_06500"
     CDS             complement(2017..5160)
                     /locus_tag="PLQ11_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003602543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRK24586.1"
                     /translation="MFTFIVTRSLRNRLFVLAFAAILTLYGALMASRLPVDVFPDLNR
                     PTVTIMTEAEGLAPPEVEQLVTYPIEAQMNGLPGVTRVRSVSGVGLSITYVEFDWNTD
                     IFRNRQQIAERLSLIQQQLPAGILPQMGPINSIMGQILLVAMTGPEDVSPMDLRETAN
                     FIVRPRLLSIPGVAQVIPIGGEVRQYRVSPNPAALRALKVTFSDVEKALQQFGANTGG
                     GFTDQYSREFMIRNIGRTRSLEDLKRTVVATVAGQPIALEQIATVDFAPRTKRGEAGY
                     QGKSAVIVSIEKQPGVDTIPLTRKIEAALGEMTATLPQGIRADQLLFRQADFIEASIG
                     NVRTVLLEAAAVVAVVLFLFLLNVRTTAISLTAIPVSILVTAIVFHFFGLSINTMTLG
                     GLAIAIGELVDDAVVDVENIFRRLRENREAGNPRSVFDVVVSASQEVRSGIVYATMII
                     VLVFVPLFALSGIEGRLFAPLGQAYIVSILVSLVVSITLTPVMAYYLLPGLKRLDHRE
                     SGLVRVLKRANRAVLMRAFRAQKALMALAGLAVMIAGYFATQLPRAFLPPFNEGTLTI
                     NLLFTPGISLAESHRVGLIAERLIMGVAEVERVGRRTGRAELDEHAEGVHSSEIEVAL
                     KPGGRGKDAVINDIRSQLAVLPVATNVGQPISHRLDHMLSGVRAQIALKIFGDDLDTL
                     RSLAETFRQQFVAVPGIVDLQVEKQVRIPQLEIIIDHRRAALYGVQPAAITEQLERLS
                     NGRIVSRVVDGNRRFDVVLRLEDHQRTTHKLSELLIETPMGWVPVRQLADVVETDGPN
                     QILRENGRRRLVVLANTDGAADMARVVTDIRAILASARLPPGFSTSLEGTFQAQEDSM
                     RTIGLLSLVSLAMIFAILYSRYRSAMFVAIIMASVPLALIGSVAALWLAGQPLSVASM
                     IGFITLTGIATRNGILKISHIINLAISDQQPLGPELILRGSLDRLTPVLMTALSAGVA
                     LLPLMIDAQAPGKEILHPVAITIFGGLVTATLLDAVLTPTLMLRYGRASLERLVAEAE
                     HGPHSAPQGQAASVEPY"
     gene            complement(5160..6617)
                     /locus_tag="PLQ11_06505"
     CDS             complement(5160..6617)
                     /locus_tag="PLQ11_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012455885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRK24587.1"
                     /translation="MILCPERFCDRHVIALAFAILSLCTPVAGHDGHDHGAPPPPVST
                     TIAPRADASSDQFEVVAVARDGKLVLTLDTFRGNAPLAGAVIELDTPQGTQKATAEPD
                     GSYALALPWLDRQGSYDLAITVQAEGVVDVLTATLTIPGSAGTGKAGTAVPASRDIAQ
                     RFADGSLFVPKHTQRILALRTEFTEQKTFRGTVELPGRIIPDPNGVGYVQASVAGRLM
                     PPPGGFPRLGTPVKAGDILAHVHPGIGAADVTSQQLQARELDQQIALVTRRLDRLRQL
                     QNVVARSQVEDAELELAGLTARRANLDRAPRHTEDLVAPVSGVIAAVNATAGLIAEPS
                     TVIFQIIDPSRFWVEALTYDVQAISGTATARLTNSRSIALAYRGMGLAERNQSVPIHF
                     SIEGETQGLRAGQFLTVLAGTAEEKAGVSLPRSAILRGANGQSLVFEHTNAERFVPRE
                     VRVEPLDAARVLVVSGLGAGRRIATQGAELLNQIR"
     gene            complement(6675..8039)
                     /locus_tag="PLQ11_06510"
     CDS             complement(6675..8039)
                     /locus_tag="PLQ11_06510"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRK24588.1"
                     /translation="MNRPSSAALFLRSLFDGLAARTIFIVLIGIGAVHLISLWTYHHA
                     LSQQAELAGDARLADQLLSMKRAVMREPQPRREAVAHDLSGGPMEAHWSLTEHATPGG
                     PGTEQWLALAERLKQAAPELDDGGLVIGANRRSADDPHLAIISMRLPDESWLNVSLVR
                     TSTRSAQGHGTLISTSLMALGAVLLSVVLVRWLTRPLTVFATAAKGFSGSATPVNVAE
                     EGPREVRALAAAFNEMQRRIARLLDERTQALAAVSHDLKTPITRLRFRTEEIADPSLR
                     AAIADDLGEMERMLDQTLAYLRGERTDEAMQPIDLVAILATICGDRSDTGADVLLGGS
                     RSATISGRRLSLKRAFGNLIDNAIKYGKKAEVTVLESPDTIEVRVADHGPGIAPEYRE
                     RALAPFVRLEPSRNQETGGFGLGLTIAQTIITGHGGTLCLSNGAEGGLIVTVRLPRHV
                     VAAG"
     gene            complement(8036..8788)
                     /locus_tag="PLQ11_06515"
     CDS             complement(8036..8788)
                     /locus_tag="PLQ11_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020665363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRK24589.1"
                     /translation="MPEGSRATTTNRQARLATVLVVDDDSEILSLVARFLQFNGFAVM
                     TARNGSEMTACLKQNSVDLIVLDLMLPGANGLDLCRDLRRWSAVPVIMLTAKGDEIDR
                     IIGLEVGADDYLAKPFNPRELLARISAVLRRTQARPSNQAAGAQRLVKFAGWRLDTTK
                     RELTNPQGIVVDLSTGEYDLLLAFLDAPQRVLSRDFLMEATRTRTLEAFDRAIDVQIS
                     RLRRKIEVDQELIKTVRGAGYMFIPDVTRGQA"
     gene            complement(8927..9002)
                     /locus_tag="PLQ11_06520"
     tRNA            complement(8927..9002)
                     /locus_tag="PLQ11_06520"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8967..8969),aa:Ile,seq:cat)
     gene            complement(9384..>11183)
                     /gene="cobT"
                     /locus_tag="PLQ11_06525"
     CDS             complement(9384..>11183)
                     /gene="cobT"
                     /locus_tag="PLQ11_06525"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019906086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobT"
                     /protein_id="HRK24590.1"
                     /translation="VTFAADKPALTDIPGAPRVRLGEPPRKPSAKDIAILRGQADSYA
                     LRLACHDMRLHRRLQPEQQQARAVFDAVEQARVDCLGARRMTGVGANITAMLEDRYLR
                     NKFDEITDREAAPIEEAVALLVRERLTGHQTPKVAASVADLWRSHIESKAGPALDALS
                     GAIDSQQTFAKAVHRLLRSLEMTDETDASPEDSKDEDNSDRQQDQNQQQAAEGDQEQS
                     PEAQELRDTERSKEDIRDGEQDEAEAPPGELDDADADLDDAEDPSEMARPPASKQPEK
                     TAVDYKVFTQKFDETVGAEELCDTEELDKLRAYLDKQLSQYHSVVARLANRLQRRLLA
                     QQNRSWEFDLEEGVLDPARLPRVVIDDLRGRFSPLSYKWEKDTDFRDTVVTLLIDNSG
                     SMRGRPITVAATCGDILARTLERCGVKVEILGFTTRAWKGGQSREAWLQAGKPAGPGR
                     LNDLRHIIYKSADSPWRRAKRNLGLMMREGLLKENIDGEALDWAHKRLLARPEQRRIL
                     MMISDGAPVDDSTLSVNAGNYLERHLRAVIHEIETRSPVELIAIGIGHDVTRYYRRAV
                     TIVDAEELGGAMTDKLAELFDERSSPAPSRRAG"
BASE COUNT         2081 a         3465 c         3634 g         2003 t
ORIGIN      
        1 aactgtgaat cacatcttcg aacaaatggg aatcccaaat ccccacgcgc cttagctcac
       61 aaccacttcg gtcatcatgc cggtggccat gtgatagagg tgatggcagt gcagatacca
      121 gctgccgggc ttgtcaaagt cgatggcgac catgacaggg gtgtgcggcg gaacaataac
      181 cgtgtcgcgc accgggccgg aaaagcgccc gctgcggctg ccaacgacct ggaaatgatg
      241 cccgtgtatg tgcatcggat gcatcatcgt ggtcggattc atgaacatga attcgatccg
      301 ctcgccaacc tttgcaagga tcggcttgtg ctcgccgtgg atcgcgccat tgatggtcca
      361 gcgatagttg gggccctcgc cgagcatgat gtgctcgatg cggcttggcg ctctggtggg
      421 cagcggatgg acggcgcgaa gcgccagatc cagcgaaaga tcggtgtgtt tggcgacatg
      481 ttccgatttt gcggaatact ttctgacggt ggcgccggct gtggccagaa tgatcccggt
      541 cagcatccgt tcggcttcca cctgggcgaa gatcggaaag gcgccaccgg atgcgggaat
      601 gtcgagcaca agatcaatcc gctggccttg ggctagcgga aagcgacgcc ccggatgggg
      661 ctggcaaggg ctgccatcca ctgcaatgca tcgggctgtc accgcgccgg tatcgatgaa
      721 gaatgcggtt gccgttgcgc cgttgatgat gcgcaggcgc agcttgctgc cacgctcgac
      781 gcggaccact tccggatcct ccagcgtgcg gtcattggca agaaaggcat cgtattggat
      841 gtcattggca tgcggctggc cggcgccatg ccccattgcc gaatgattgg cgccgccatg
      901 ctgcatgcca tgtccggcgt gggcgccctg accgcccgcc tgtgccggcg cggccggtgt
      961 agcgccatgc ccggcatggc cagaatgagc attgacgccg cccaggcctg ccagcagttc
     1021 cgccggcgga cggaaggaaa agtcgtgcag cataactatc acatcctgca catcgccggc
     1081 gtccttctcg cgcgtcacca tgggagccgc cagaagctgc tgttcggtca gggtgtgcga
     1141 gtgcatccag tgcgtgcccg gcgtgagtcg gaagtcgtgc agatcactct gtccgggcgc
     1201 cagttcgccg ccccctgtgc gggcgcgatc ctgttcggcc ggcgcataaa tctgtccatg
     1261 ccagtgcatg acgagcggtt cggccgttgc attgagcaac gcgccggaga aacggtcgcc
     1321 ctcccgggcc aggattccgt gacgcccatt ggccccggcc acgccaaaca cggtcgcgcc
     1381 gcgcctcccg acctcaatct gccgggtggt gacggtcaat agcggggatt gcgcgctggc
     1441 cggcacgcgg gcggcggcca ggcctgtgac tgtggcggct ccggcgagaa acagacgacg
     1501 atccatggtg cacctcgata tcgggctggg ttagagcatt ttcaagcgaa gtggagtccg
     1561 gttcgcttta agaaaatgcg aaaaaacaag aagatagagc actttgacgc ggattggatt
     1621 tcgctgagag tgctctagaa ctttgcctgg gaggttgtcc cgtccggcgc cgtgagctgg
     1681 acggcgcctt tgctgccggc gggcacggcg accggtgccc gtccgacaag gcgccccccg
     1741 tcggtcggct caagcgcaaa gcgttgcggc ttgccatcga caacaatgat ggcgctggcc
     1801 ttgaagccgg tagaagcaac aggcttgtca gcggaatccg acaggatcaa catcacctcc
     1861 ggcgtccctg tggcaatcag ttcgatatga tagctcttgc cggcatcgac cagagcgccg
     1921 ccgttcggtc ctttgcgcgg ttcgtgggca aaggcaaggc tgccggcgag aaacacggca
     1981 gcggtgagtg ttatgcgcat ggcaggctcc ttgctctcaa tagggttcaa cgctggcggc
     2041 ctgaccctgc ggcgcagagt ggggcccatg ttcggcctcg gcgaccaggc gctccagtga
     2101 agcgcggcca tagcgaagca tcagggtggg ggtcagcacc gcatccagca gcgttgccgt
     2161 taccaacccg ccgaagatcg tgatggcgac ggggtggagg atctccttgc ccggcgcctg
     2221 cgcgtcgatc atcagcggca gcagtgcgac gcccgcagac agtgcggtca tcaacaccgg
     2281 ggtcagccga tcgaggctgc cgcgcaggat cagctctggc cccagcggct gttgatcgct
     2341 tatcgccagg ttgatgatat ggctgatctt cagaatgccg ttacgggtgg cgatgccggt
     2401 cagcgtgata aagccgatca tcgaggccac cgagagcggc tgccccgcca gccacaaagc
     2461 cgccaccgat ccgatcagcg ccagtggcac ggaggccatg atgatggcca cgaacatggc
     2521 cgagcggtaa cgactgtaga gaatggcgaa gatcatcgcc agcgagacca gcgacagcag
     2581 gccgatagtc cgcatggagt cttcctgcgc ctgaaaggtg ccttcaaggc tggtcgagaa
     2641 gcccggcggc aatctggcac tcgccagaat cgcacgaatg tccgtcacga cacgcgccat
     2701 gtccgccgcg ccatcggtat tggcgagaac gaccagtcgg cggcgcccgt tttcccgcag
     2761 gatctggttc gggccatcgg tttcaacaac atcggcaagc tggcggaccg gcacccaacc
     2821 catcggtgtt tcgatcagca gctcactgag cttgtgggtg gtgcgctggt gatcctcgag
     2881 ccgcagcacc acatcgaagc ggcggttgcc gtcgacaacg cgggagacga tgcgtccatt
     2941 cgacaggcgc tccagttgct cggtgatggc ggccggctgc acgccgtaaa gtgcggcgcg
     3001 gcgatgatcg atgatgattt ccagttgcgg gatgcggacc tgcttttcaa cctgcagatc
     3061 gacgatgcct ggcacggcaa cgaattgctg gcgaaaggtc tcggccagcg aacgcagggt
     3121 gtcgagatcg tcgccgaaga tcttcaatgc aatctgcgcc ctgacacccg acagcatgtg
     3181 atcgagacga tgcgagatcg gctggccgac attggttgcc acgggcagga cggcaagttg
     3241 gctgcgaatg tcgttgatca ccgcatcctt gccgcgcccc ccgggtttga gagccacttc
     3301 gatttcggag gaatgcacac cctcggcatg ttcgtcgagt tccgcccgtc cggtgcggcg
     3361 gccgacgcgt tccacttcgg cgacacccat gatcaaccgt tcggcaatca ggccgacgcg
     3421 atggctctcg gccagcgaga ttccgggcgt gaacagcagg ttgatcgtca gcgtgccctc
     3481 gttgaacggc ggcagaaagg cgcgggggag ctgcgttgcg aaatagccgg cgatcatgac
     3541 agccaggccg gcgagcgcca tcagcgcctt ctgcgcccgg aacgcccgca tcagcacggc
     3601 ccggttggcc cgcttgagca cgcgcaccaa cccgctctcg cggtggtcga gccgcttgag
     3661 gccgggcaac agatagtagg ccatgaccgg ggtcagggtg atcgatacga caaggctgac
     3721 aaggatcgaa acgatatagg cctgcccgag cggggcgaac aggcggccct cgatccctga
     3781 aagggcgaac agcggcacaa acacaagaac gatgatcatc gtcgcataga cgatgcccga
     3841 gcggacctcc tggctggcgg agaccacaac gtcgaacaca ctgcgtggat tgccggcctc
     3901 gcggttttcc ctgagccggc ggaaaatgtt ctcgacgtca accacggcgt catcgacaag
     3961 ctcgccgatg gcgatggcaa ggccgccaag tgtcatggtg ttgatcgata atccaaagaa
     4021 gtgaaacacg atggccgtca ccaggatcga caccgggata gccgtcagcg agatcgccgt
     4081 ggtgcgcacg ttgaggagaa acaggaacag caccaccgcg accacggcgg ccgcctccaa
     4141 gagcacggtc cggacgttgc cgatcgaggc ttcaatgaag tcagcctgcc ggaacagcag
     4201 ctgatcggcg cggatgccct gaggcaatgt ggcggtcatt tcgccaaggg ccgcttcgat
     4261 cttccgggtt agagggatgg tatcaacccc aggctgcttt tcgattgaaa caatgacggc
     4321 ggacttgccc tgatagccgg cttcgccacg cttggtccgg ggtgcgaaat ccaccgtggc
     4381 gatctgctcc agagcgatcg gctggccagc cactgtcgca acaacggtgc gtttgaggtc
     4441 ctcaaggctg cgcgttcggc caatgttccg gatcatgaac tcacgcgaat actggtccgt
     4501 gaacccgcca ccggtattgg cgccgaactg ctgcaacgcc ttctccacat ccgaaaaagt
     4561 gactttcagt gccctcagcg ccgccggatt gggcgagacg cggtattgcc ggacctcgcc
     4621 gccgatcgga atgacctgcg ccactccagg aatagacaaa agccgggggc ggacaatgaa
     4681 gttggccgtc tcgcgcaggt ccatcggcga aacgtcctct ggcccggtca tggcgaccag
     4741 caggatctgc cccatgatgg aattgatcgg tcccatctgc ggcaggatgc cggccggcag
     4801 ctgctgctgg atcagcgaca gccgttcggc gatctgctgg cgattgcgga agatgtcggt
     4861 gttccagtcg aattcgacgt aggtgatcga caggccaacg ccggaaaccg atcgcacgcg
     4921 cgtcacaccg ggcagcccgt tcatctgggc ctcgatcgga taggtcacca gctgttcgac
     4981 ctcaggcggg gccagccctt ctgcctcggt catgatcgtg acggtcggcc ggttgaggtc
     5041 gggaaacacg tcaaccggca gccgtgaggc catcagggcg ccatagaggg tcagaatcgc
     5101 ggcgaaggcc aggacgaaca gccggttgcg caaggatcgg gtgacgatga aggtgaacat
     5161 cagcggatct ggttcaacaa ttcggcgccc tgggtggcga tccgccggcc ggctccgaga
     5221 ccggagacca ccagcacccg tgcggcatcc agcggttcga cacgaacctc gcgcggaacg
     5281 aagcgctcgg cattggtatg ttcaaacacc aagctttggc cgttggcgcc gcgcaggatg
     5341 gccgaacggg gcagtgaaac gccagccttt tcctcggccg tgccggcaag gaccgtcagg
     5401 aactggccgg cccgcagccc ttgcgtctct ccctcgatgc tgaagtggat gggaacggat
     5461 tggttgcgtt ctgccagccc cataccgcga taggccagcg caatcgaacg gctgttggtc
     5521 aggcgcgccg tcgcggtgcc ggaaatcgcc tgcacgtcgt aggtcagggc ctcgacccag
     5581 aaccgggagg ggtcgatgat ctggaaaatc acggtcgatg gctcggcgat cagtccggcg
     5641 gttgcgttga ctgcggcgat gacaccgctg accggagcga ccaggtcctc cgtatggcgc
     5701 ggggcgcggt cgagattggc gcgccgcgcc gtcagtcctg caagttccag ttccgcatct
     5761 tcgacctgcg agcgggcgac gacgttctgc aattgccgga gtcgatcgag ccggcgggtg
     5821 acaagggcga tctgctggtc aagctcgcgg gcctgcagct gctgtgaggt cacgtccgcc
     5881 gcgccaatgc cgggatggac atgggccagg atgtcgccgg ccttgaccgg cgtgccaaga
     5941 cgcggaaaac cgccgggcgg cggcatcagc cgtccggcca ccgaagcttg aacataccca
     6001 acaccattgg gatcgggaat gatccgcccc ggcaattcga cggtgccgcg aaaggtcttc
     6061 tgttcggtga attcggtgcg cagggcgagg atgcgctggg tgtgcttggg cacgaaaagg
     6121 ctgccgtcgg cgaaccgctg ggcgatgtcg cggctggccg ggaccgccgt tccggccttg
     6181 ccggtcccgg cggaaccggg aatcgtcagg gtcgccgtca gaacatcgac caccccttcc
     6241 gcctgcacgg tgatggcaag gtcatagctg ccctgcctgt ccagccaggg caaagccagc
     6301 gcatagctgc catcgggctc ggctgtggcc ttctgtgtgc cctgaggggt gtcgagttcg
     6361 atcacggcgc cggccaacgg cgcattcccg cggaatgtgt cgagcgtcag caccagtttg
     6421 ccgtcgcgcg ccaccgccac gacctcgaac tgatccgaag aggcgtctgc tcgcggagca
     6481 atcgtggtgg aaacaggcgg cggcggggcg ccatgatcat gcccgtcatg cccagccacc
     6541 ggggtgcata acgacaggat ggcaaaggcc agggcaatga catggcggtc gcagaagcgc
     6601 tcggggcaca gaatcatccg ccaaggatga aggccttcct tgtcctgcgg atttcaggat
     6661 tgttacagaa tgtttcagcc ggcagcgacc acatgtcgtg gtaaacggac tgtgacgatc
     6721 aatccaccct cggcgccgtt cgacaggcaa agggttccgc catggcctgt tatgatcgtc
     6781 tgggcgatcg tcaggcccag gccaaacccg cccgtctcct ggttgcgcga gggttccagc
     6841 cgcacgaacg gggccagggc ccgctccctg tactcggggg caatgccggg gccgtgatcg
     6901 gcaacgcgca cctcgatcgt atcggggctt tccaggaccg ttacctcggc tttcttgccg
     6961 tatttgatgg cattgtcgat caggttgccg aaggcgcgct tcaatgacag cctgcggccg
     7021 gaaatcgtgg cgctgcggct gccgccgagg agcacatcgg ctccggtatc gctccggtcg
     7081 ccacagatcg tggcaaggat cgccaccagg tcaatgggct gcattgcttc atcggtgcgc
     7141 tcgcctctga gataggccag cgtctggtcg agcatgcgtt ccatttcgcc aaggtcatcg
     7201 gcgattgctg cgcgcaggct cggatcagcg atttcctcgg tccggaacct cagccgggtg
     7261 atcggtgtct tgagatcgtg tgacaccgct gccagggcct gcgtgcgttc atccagcaat
     7321 cgcgcgatgc gccgctgcat ttcattgaag gcggcggcca gagcgcggac ctcgcgcggc
     7381 ccttcctcgg cgacattgac gggcgttgcc gatcccgaaa agcccttggc cgccgtggca
     7441 aagacggtga gcggccgcgt cagccagcgc accagcacca ccgacaacag caccgcgccc
     7501 agtgccatca gcgatgtgga gatcagcgtg ccatgccctt gagccgaacg ggtcgaggtc
     7561 ctgaccaggg aaacattcag ccagctctcg tcgggcagcc gcatcgagat gatggccaga
     7621 tgcgggtcat cggcgctgcg gcggttggcg ccaatcacca gcccaccgtc gtccagttca
     7681 ggagcggcct gcttcagccg ttcggccagc gccagccatt gctcggtgcc gggcccgccg
     7741 ggcgttgcat gctcggtcag gctccaatgt gcttccatcg gcccgcctga aaggtcatgt
     7801 gccacggcct cgcggcgtgg ctgcggttcc cgcatgacgg ctcgtttcat gctgagcagc
     7861 tggtcagcca gccgcgcatc gccggccagc tcggcctgct gcgacaatgc gtggtgatag
     7921 gtccagaggc tgatgaggtg gacggcgccg atgccgatca acacgatgaa gatggtccgc
     7981 gccgccagcc cgtcgaacag ggatcgcaga aacagtgcag cggaggaggg gcggttcatg
     8041 cctgtccccg cgtcacgtcg ggtatgaaca tatagccggc cccgcgcacg gtcttgatca
     8101 gctcctggtc cacttcgatc ttgcggcgca atcggctgat ctggacgtcg atggcacgat
     8161 caaacgcctc cagcgtccgc gtgcgggtcg cctccatgag gaaatcacgg gacaggaccc
     8221 gctgtggcgc atcgaggaat gcgagcagca gatcatattc gccggtcgac agatcgacga
     8281 caatcccttg cggattggtc agttcgcgct tggtcgtatc gagccgccag ccagcgaact
     8341 tgaccagccg ctgcgccccc gccgcctgat tggacgggcg tgcctgagta cgcctgagca
     8401 cagcgctgat tcgggccaga agctcgcggg ggttgaatgg cttggcgaga taatcgtcgg
     8461 cgccgacttc cagcccgatg atccggtcga tttcatcgcc cttggccgtc agcatgatga
     8521 ccggaacagc agaccagcgt cgcaggtcgc gacaaaggtc aaggccattg gcaccgggca
     8581 gcatcagatc caggacgatc agatcgaccg aattctgctt caggcaggcg gtcatttcgc
     8641 tgccattgcg ggcggtcatc accgcaaagc cattgaattg aagaaagcgg gctacaaggc
     8701 tgagaatctc actgtcgtcg tccacaacca ggaccgtggc caaccgcgcc tgacgatttg
     8761 ttgttgtggc ccgggaccct tcaggcatgg gaaaaatccg cgcaatcaac tgaaccgatg
     8821 aaatcccatc ttgaagtgct tggtcctgcc tgtcatccaa tatgtccgga aactcggcat
     8881 ccagatcgat ccgcagctgc ggaagggcag gttcgaccat caagactggt gggccctgca
     8941 ggattcgaac ctgcaaccag accgttatga gcggccggct ctaaccgttg agctaagggc
     9001 ccggcgcaat cctcttgtca tattcgcccg gtggctggca aggcggctgc tgcgtcagtc
     9061 gccgccgagg agctagagcg tttttccttc atacggaagc aattctgttt ctcgggcggg
     9121 gcggcgatcc aggcggaggg cggcgcagag cgtatccgtc gatacggtca agccggcttc
     9181 caaactgggc gcccgccgcc cggaaatccg aaggacgggc aggatttggg ccaaatactc
     9241 ggaaaatccc ctcgaccata cacggggtat gctcttcggg aatttcccgg cccttcgccc
     9301 aaaccctgca ccgcagaatg attccgtatg aaggaaaaat gctctgggca aatgaccgtc
     9361 cggcagagca gcccgatctg ccgtcaaccc gcacggcgcg acggtgccgg tgagctgcgt
     9421 tcgtcgaaca attcggccag cttgtcggtc atcgcgccgc ccagttcctc ggcatcgaca
     9481 atcgtcaccg cccgccggta atagcgggtg acatcgtggc ctatgccgat ggcgatcagc
     9541 tccaccggcg agcgggtctc gatctcgtga atgaccgccc tgagatggcg ttcgagataa
     9601 ttgccggcat tgaccgaaag ggtggaatca tcaaccggcg ccccgtccga gatcatcatc
     9661 aggatgcgcc gctgttcggg gcgcgccagc agccgcttgt gggcccagtc gagcgcttcg
     9721 ccgtcgatgt tctccttgag caggccctcg cgcatcatca ggccgaggtt gcgcttggcg
     9781 cggcgccagg gcgaatcggc ggatttatag atgatgtggc gcagatcgtt gagccggccg
     9841 ggtcctgccg gcttgccagc ctgcagccag gcttcgcggg attgcccccc tttccaggcg
     9901 cgggtggtga agccgagaat ttcgaccttg acaccgcagc gctccagcgt gcgggcgaga
     9961 atgtcgccgc aggtggcggc cacggtaatc gggcggccgc gcatcgaacc ggaattgtcg
    10021 atcagcagcg tgaccacggt atcgcggaaa tcggtgtcct tctcccattt gtaggacaaa
    10081 ggcgagaagc ggccgcgcaa atcgtcgatg acgacgcgcg gcaggcgtgc cgggtcaagc
    10141 acgccttctt cgaggtcaaa ctcccaggag cggttctgct gggcaagcag acggcgctgc
    10201 aggcgattgg ccagccgcgc gacaaccgaa tgatactggc tgagctgctt gtcgagatag
    10261 gcccggagct tgtcgagctc ttcggtatcg caaagctcct cggcgccgac cgtctcgtcg
    10321 aatttctggg taaacacctt gtaatcgacg gccgtctttt ccggctgctt gctggcgggc
    10381 ggacgggcca tttcggacgg gtcttcggca tcgtcgagat cggcgtccgc atcatcgagt
    10441 tcaccgggag gggcctcggc ctcgtcctgt tctccgtccc ggatgtcctc tttcgaccgc
    10501 tccgtatcac gcagctcctg tgcttcgggt gattgctcct ggtcaccctc ggcggcctgc
    10561 tgctggttct ggtcctgctg gcggtccgaa ttgtcctcgt ccttgctgtc ctcgggcgaa
    10621 gcatcggtct cgtcggtcat ctccagcgag cgcagcagcc gatgaacagc cttggcaaag
    10681 gtttgctggc tgtcgatagc gcctgacagg gcatcgagcg ccggtccggc cttgctctcg
    10741 atatgcgagc gccacagatc ggcaacgctg gcggccacct tcggcgtctg gtgaccggta
    10801 agccgctcac gcaccaacag cgccacagcc tcctcgatcg gcgcggcctc gcgatcggtg
    10861 atctcgtcga acttgttgcg aagatagcga tcctcgagca tggcagtgat attagcgccg
    10921 acgccggtca tgcggcgcgc acccaggcaa tccacccgtg cctgctccac cgcatcgaaa
    10981 accgcacgcg cctgctgctg ctcgggctgc aggcggcggt gcagacgcat atcgtggcag
    11041 gccagacgca aggcgtagga atcggcctga ccgcgcagga tggcgatgtc cttggccgaa
    11101 ggcttgcgcg gcggctcgcc gaggcgcacc cgcggcgcac cgggaatatc ggtcagcgcc
    11161 ggcttgtcgg cggcgaaggt cac
//
LOCUS       DAOMXG010000073        10908 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_3222_length_10908_cov_11.252925, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000073 DAOMXG010000000
VERSION     DAOMXG010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 10908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10908
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3222_length_10908_cov_11.252925"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            187..711
                     /locus_tag="PLQ11_06530"
     CDS             187..711
                     /locus_tag="PLQ11_06530"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010967265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HRK24591.1"
                     /translation="MIPPASATPRLLIVMGVSGSGKSSVGTGLAAALGGHFLDGDAFH
                     PPASIAKMRRGEALTDADRWPWLDSLAKTMAQTPGIVIGGCSALRRIYRDRIIAAAGE
                     PVLFLHLAGPKDLIWQRMAARTDHFMPVSLLDSQFATLEPPGPDEPALCVDIAPPLAE
                     VVTRLARRLRPRHA"
     gene            795..1094
                     /locus_tag="PLQ11_06535"
     CDS             795..1094
                     /locus_tag="PLQ11_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24592.1"
                     /translation="MSSHRPMLRMMFTNWALGIALGLGFAALMLAFDIGGLWSLIQRS
                     DSGWIAAALLAGGFSVTCGGVVCASAIMRMPREEDDPTPGLPELAEPALAQARRR"
     gene            1202..1462
                     /locus_tag="PLQ11_06540"
     CDS             1202..1462
                     /locus_tag="PLQ11_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002971390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24593.1"
                     /translation="MEGLLIQLISGAVGGNVAGALLKNISLGTLGNSIAGIVGGGLGG
                     SVLSSLIPILAQGIGGQIAGGGVGGAIIMAVIGMIKNAMANR"
     gene            complement(1578..3086)
                     /locus_tag="PLQ11_06545"
     CDS             complement(1578..3086)
                     /locus_tag="PLQ11_06545"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020092093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HRK24594.1"
                     /translation="MNAHVKIADVVDEVPAAGQPEPPAVAVVHSPAVAPDIRLSQPMV
                     IGTVLATDVVMLLAGGLASEAMIGSGWNAGPVVSLGLAMAAVICLLHQRFFGYTIASF
                     GSAVQQILRFGGAMVSAFAALVTFIVIMDSDVGSVRGWFIGWAALAFGVIACGRIVLA
                     RQVGRWTEQGRLARRAVIVGGGRPVEDLLASLARDGSRSIDILGLFDDRGGERSPDQV
                     DTYHKLGRFDELVAFCRDHRVDLLIITLPHVAEARILHLLKKLWVLPIDIRISAVGAK
                     LKLRPRAYTYIGKTPFLAVFDKPLTDWGIAVKAVEDRVLAGLILVLLAPVMLAVALAV
                     KLTSKGPVLFKQTRYGFNNEKIGVYKFRSMYVDQCDANAVKLVTKGDPRVTKVGAFIR
                     KTSLDELPQLINVLKGELSIVGPRPHAMQAKAGGQIYDEVVEGYFARHKVKPGITGWA
                     QINGWRGETDTEEKIQRRVEYDLQYIDNWSVFFDLYILAMTPVSLINTKNAY"
     gene            3327..5699
                     /locus_tag="PLQ11_06550"
     CDS             3327..5699
                     /locus_tag="PLQ11_06550"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3772 domain-containing protein"
                     /protein_id="HRK24595.1"
                     /translation="MTRALAILWLAFGLGWAAVAQTPAPRTDMEALRQELSQIEALLK
                     RGTLADSELIRLRTRLEPIAGEVGEILAREQPRLDDINARLSQIGPKPDDKAPPESPE
                     VARTRLEQETLKKEVDEAIRVARLLQVRTDQAGAAIIDQRRSLFRQEVLARSQSVLSP
                     WLWADVLSAIPAAMSDLHASIGFRLRHSADAYDWRSLAAALALLLIGAAGAVPAHRIL
                     RQWDAVTESNQAPGRLDKALAALRHALAVSIVPLAFALLLTAAQEIILPHHERFSHML
                     RTAVFSVPLVLFLRGLTHGILSPTRPVWRLFALSDVAARRITATVTQLLMLSAFGKIV
                     EAANQAISAILPFTVATRGMVALVSGSVLLRLLRALRRPQETTAGDDEFAGTVSAMVP
                     ASLAALALAMIITALLGYVALASLLVDQLLWGMAIAALIGLVLIVVDEVLARGRLSES
                     RPARQLRVLTGIDIGSMRQAATLSSGVVKLLLYILAVLMFLAPLGVNSDDMLGSLRAA
                     FFGFQLGGVTISISTLVLALSIFVLAVFVTRSIRRWLEGTFLPTTSLDTGLQNSILTV
                     LGYIGFIIGAVAAMGTLGLGFDKITIVAGALSVGIGFGLQSIVNNFVSGLILLWERPI
                     RVGDWIVVGEEQGKVQRINVRATQIETFDKASLIVPNSEFISGRVKNFMHANRTTRII
                     IPVGVSYDSNPETVRHLLLDAAMAHREVMSDPAPNVLFISLGESSLNFELRCFVDVDA
                     MATTRSDLMFAIFEALRREKITVPFPTRTVEIADMNGLAGAIAARMPKKG"
     gene            complement(5703..6944)
                     /locus_tag="PLQ11_06555"
     CDS             complement(5703..6944)
                     /locus_tag="PLQ11_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010685905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRK24596.1"
                     /translation="MKDSGKTGEPSGGWAVFAEPDFRAFALARLMSGMAQNIQTVGVG
                     LYVYQVTRDPLALGLAGLFTFLPQLMFVALSGHAADSFDRRRIVAAGYALAAVSGVGL
                     MLIAGWGLTATAPVYAMITLMGTTQAFVLPANQAMLPNLVRPGWQASAVAWNASIRQA
                     ATILGPMAGGFLYLLGPLATFAVACLCNLLGVAAALTITPQRSPARREAFSFSAFTTG
                     ARFITANKVLLGALSLDLVAVLFAGVVALLPIYATDILEAGPAALGWLRASQAAGALG
                     MALFLARYPIRRHGGRWMLTASAVFGLAIIGFGLSRLLWLSMLMMMIEGAADMVSVVV
                     RGTLVQAETPDDMRGRVGSVNSLFIGASNSLGDFESGLVAAWIGVVPATIVGGCLSLM
                     ATALWCVLFPELRKRDRLQKG"
     gene            complement(7062..7883)
                     /locus_tag="PLQ11_06560"
     CDS             complement(7062..7883)
                     /locus_tag="PLQ11_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HRK24597.1"
                     /translation="MARKKGGGHGGGHGWFVTFADLMGLLMSFFVMLTAFSTQDQKKL
                     QIVAGSMREAFGTQKQSKLSGIVEIEGLPVRPHMRYVERTTPDKSSEFTGPRQTEKPT
                     PSSALSGSGDRALSQAVATLRQAMQDLPEIAEVSKNVLIEETAEGFDIQIVDQDGRAM
                     FGEGEARPNDRATKILDMITDKISQLANPIRITGHSSSSRLNVQEAGGWAVSTQRALI
                     VRERLAGRGIPRHRFESVAGKADSEPLFQGDPGLSANRRVSIVLVRMAPPLPPAK"
     gene            complement(7896..8663)
                     /locus_tag="PLQ11_06565"
     CDS             complement(7896..8663)
                     /locus_tag="PLQ11_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRK24598.1"
                     /translation="MDISTILGLVVGMVTVAALILMGGNFGMFYDVHAIIVIGGGCAA
                     GTLIRFPLSAMKHGFPMGLKYAFKMRARGPRQLIEDITQIAETYRKGGGTALDQIVVE
                     DSFLKQAVRYLADGYDADFIRRTLEQERDTFFQHLDEGQKIYRSIGDAAPAWGMVGTL
                     VGMVQMFSNMEDPSKLGPFMAVALLATLYGALAGNLICLPIADKLHVKLEEEDVSRNL
                     IIDGVLQMRASKSPAVIREMLISYLPEHHKHDFVEAA"
     gene            8820..10337
                     /gene="gpmI"
                     /locus_tag="PLQ11_06570"
     CDS             8820..10337
                     /gene="gpmI"
                     /locus_tag="PLQ11_06570"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010684929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HRK24599.1"
                     /translation="MAYSPVMLVILDGWGERAETADNAVRQARTPNFTRLWNAHPHAL
                     LNTSGLDVGLPGGQMGNSEVGHLNIGAGRVVMQDLPRIGQAIADGSLAASPAVARLIA
                     ALKMSGGTCHLIGLISNGGVHSHQDHALALARIVTAAGVPVVLHALTDGRDTPPQSGL
                     AFVREVEAALPAGARIGTIIGRYYAMDRDNRWERVSKAYDAMVSGAGTAFASAGAAIE
                     AAYASKVTDEFILPAIIAGYPGMGDGDGLLSFNFRADRVREILSAMVDPAFTGFERSR
                     IVRLAARVGMAQYSTALDAHLETIFAPQNLANVLGAAVAAAGKRQLRMAETEKYPHVT
                     YFLNGGEEAVYPGEDRIMVPSPKVATYDLQPEMSAPELTDKAVAAIDSGTYDLIVLNF
                     ANPDMVGHTGSLPAAIRAVETVDAGLGRIADAIARQGGALLFTADHGNCELMKDPVTG
                     GPHTAHTTNPVPACLTAGARRLRDGGRLADLAPTLLQLMGIAQPKEMNGRSLIAE"
     gene            complement(10377..>10908)
                     /locus_tag="PLQ11_06575"
     CDS             complement(10377..>10908)
                     /locus_tag="PLQ11_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24600.1"
                     /translation="TTTEVEGGWVKNSSGMTKVVYINDANGNRIGGYYVNYDASGKET
                     GRQHFTESGGAQPQTASAPVNPALEGTYSGRIAGGSSGSLTFTVSGGQVSGTIGGVHE
                     GDPINASFSGPLSAEGTFSAAANGVLRTTWADGKTSTFTFTGKIGGKVNGRSGSGTWS
                     GSNRWGASKGTWSASK"
BASE COUNT         1936 a         3601 c         3373 g         1998 t
ORIGIN      
        1 tgaatcctga atgtcgattg gtcctagagc atgtcgcgat taagtggata ccggttaatc
       61 gcagaagaca tgctctaact tattgatacc gatcacgtta tacgcatttt gatgattcca
      121 tcaaaatgcg gcgtgatcta aggatttcat gcgccccggc ccctacggcc gttccggaga
      181 ccgtgcatga tccccccagc atccgcgacg ccccgcctct tgatcgtcat gggtgtctcc
      241 ggctcgggaa agtcatcggt cgggacgggg ctggccgcag ccctcggcgg ccactttctt
      301 gatggtgacg cctttcaccc ccctgccagc atagccaaga tgcgccgcgg cgaagcgctg
      361 accgacgccg atcgctggcc ctggctcgac agccttgcca agaccatggc gcaaacaccc
      421 ggcatcgtca tcggcggctg ctccgcgctc aggcgcatct atcgcgaccg gatcatcgca
      481 gctgccggcg aaccggtgct gttcctccat cttgccggcc cgaaggacct gatttggcag
      541 cgcatggcgg cccgaacgga tcatttcatg ccggtctcgc tgctcgacag ccagtttgcc
      601 acgctggaac cgcccggccc ggacgaaccc gccctttgcg tggatattgc gccgccgttg
      661 gcagaagtgg tcacccggct cgcccgccgg ctgcggcctc ggcacgcctg accgtggagc
      721 aatgcaacca agggcttggc aaatcggcat ttcgggagtt caatagtgcc ggttcaggtc
      781 tgccggaggt gtccatgtcc tcgcatcgcc ccatgctccg catgatgttt accaactggg
      841 cgctcggcat cgcgctcggc ctgggctttg ccgcgctgat gctggccttc gatatcggcg
      901 gtctctggtc gctcatccag cggtccgatt caggctggat tgcggcggcc ctgctcgccg
      961 gagggttctc ggtcacctgc ggtggtgtgg tctgtgcttc ggcgatcatg cgcatgccgc
     1021 gcgaggagga tgaccccacg cccggtcttc cggagcttgc cgaaccggcg ctggcccagg
     1081 cgcgccgccg ctgaggcagc gaatacccgg acgatggggt aatttttgct tgcagttcag
     1141 atataaacag cctcagatat gctcagtctg cgcctaagtg gcgcttcttg ggaggatgga
     1201 aatggaagga ctgcttatcc aactgatttc gggtgccgtc ggcggaaacg tggcgggcgc
     1261 gctgctcaag aacatcagcc tcggcacgct tggcaactcg atcgccggca ttgtcggtgg
     1321 cggccttggc gggtcggtcc tgtcgtcgct gatcccgatc ctggcacagg gcattggcgg
     1381 tcagatcgcc ggcggcggtg tcggcggcgc catcatcatg gcggtgatcg gcatgatcaa
     1441 gaacgcgatg gccaatcgct gacaatgcga tgtccggctg gaaagccgct gcgggcccat
     1501 cggccagcgg cggctttttg gtctgtttgc tggagagggt ggatcgatag ccgcccgtgt
     1561 cacagcccga gccatgctca ataggcgttc ttggtattga tcagcgacac cggcgtcatc
     1621 gccaggatat agagatcgaa gaacacgctc cagttgtcga tatactggag atcgtattcg
     1681 acacgccgct ggattttttc ctcggtgtcg gtttccccgc gccagccgtt gatctgggcc
     1741 catccggtga tgccgggttt gaccttgtgg cgggcgaaat agccctccac cacttcgtca
     1801 tagatctggc cgccggcctt ggcctgcatg gcatgcggcc ggggaccgac gatcgacaat
     1861 tcgcccttga gcacattgat gagctggggc agttcatcga gcgaggtctt gcggatgaag
     1921 gcgccgacct tggtcacgcg cggatcgccc ttggtgacca gcttgacggc attggcatca
     1981 cactggtcga catacatcga tcggaacttg tagacaccga tcttctcgtt gttgaagcca
     2041 tagcgcgtct gcttgaacag caccggcccc ttggacgtca gcttcaccgc cagcgccacc
     2101 gccagcatca ccggcgccag cagcacgagg atgaggccgg ccagaacccg atcctcgacg
     2161 gctttgaccg cgattcccca atcggtcagc ggcttgtcga acacagcaag gaacggcgtc
     2221 ttgccgatat aggtataggc gcgcggccgg agtttcagct tggcacccac cgcgctgatg
     2281 cggatgtcga tcggcagcac ccacagtttc ttgagcagat gcaggatgcg ggcttcggcc
     2341 acatgcggca gggtgatgat gagaaggtca acccgatgat cacggcagaa ggcgacgagt
     2401 tcatcgaagc gaccgagctt gtggtaggtg tccacctggt cgggcgagcg ttcgccgcca
     2461 cgatcatcaa acaggccgag gatatcgatg gagcgcgacc catccctggc gagtgaggcc
     2521 agaagatcct cgaccggtcg gccgccgccg acaatcaccg cacgacgggc caggcggccc
     2581 tgttcggtcc agcgcccgac ctgccgggcc agcacaatgc ggccgcaagc aatgaccccg
     2641 aaggccagcg ctgcccagcc gatgaaccag ccgcgcaccg accccacatc cgaatccatg
     2701 atcacgatga acgtcaccag cgcggcaaag gccgagacca tggcgccgcc aaacctcagg
     2761 atctgctgga cagcactgcc gaaggacgcg atggtgtaac cgaagaaccg ctggtgcagc
     2821 aggcagatga ccgccgccat cgcaagaccc agcgagacaa cgggcccggc gttccaaccg
     2881 ctgccgatca tcgcctcgct cgcaaggccg ccggccagca gcatcacgac atccgtcgca
     2941 agcactgtac cgatgaccat cggctgcgac agccggatat ccggggccac cgcaggactg
     3001 tggaccacag cgaccgctgg cggctccggc tggcccgcag ccggaacctc gtcaaccaca
     3061 tcggcaatct tcacatgggc attcatcgaa aaatccaccc gctgcatcgc gccggccgcg
     3121 cccccacccc ggcatcagat gtgatggcac agtgggttcg aagaagggtt aagcggaccc
     3181 gcaaagcacg gcccaatcgg cccgcgaccg ctggaattgg ttatcaagtc cttggcgagg
     3241 catggccaac ttgcgcttat gcctcacgga aaagccgttt atgcctggtc tgagggaccg
     3301 attcgcaacg tcacggggaa caggtcatga cacgcgcgct ggcaattctg tggctggcct
     3361 tcggccttgg ctgggcggct gttgcccaga ctcccgcccc tcgcacagac atggaagcct
     3421 tgcgtcagga gttgagccag atcgaggcgc tgctcaagcg gggcacgctc gccgacagtg
     3481 aattgatccg cctgcgcacc cgccttgaac cgatcgccgg agaggtgggg gagatactcg
     3541 cccgcgaaca gccacgcctc gacgacatca atgcgcgcct ttcccagatt ggccccaaac
     3601 ccgatgacaa ggcgccgccc gaaagcccgg aggttgcccg aacccgcctg gagcaggaaa
     3661 cgctgaaaaa ggaggtggac gaggcgatcc gcgtcgcccg cctgctgcag gtgcggaccg
     3721 atcaggccgg agcggccatc atcgaccagc gccgcagcct gttccgccag gaagtgctgg
     3781 cccgttccca gagtgtgctg agcccatggc tgtgggcgga cgtgctgagt gccattcccg
     3841 ccgcgatgtc cgatctgcac gcatcgattg gcttccgcct gcgccatagc gccgacgcct
     3901 atgactggcg cagccttgcg gcggcgcttg ccttgctgct gatcggcgcg gccggcgccg
     3961 ttcctgctca tcggatcctg cgccaatggg acgcggtgac ggagagcaac caggcgccgg
     4021 gacggctcga caaggcgctc gcggcgctgc gccatgcgct ggccgtttcc atcgtgccac
     4081 tggcctttgc cctgctgctg accgccgcgc aggagatcat cctgccgcat catgagcgtt
     4141 tcagtcacat gctgcgcact gcggtgtttt ccgtgccgct ggtgctgttc ctgcgtggcc
     4201 tgacgcatgg catcctttcg ccgacccgac cggtctggcg cctgttcgcc ttaagcgatg
     4261 tcgcggcccg gcgcatcacg gccacggtga cgcaactgct gatgctgagc gccttcggca
     4321 agattgtcga agccgccaat caggcgattt cggcgatcct gccctttacg gtcgcaacgc
     4381 gcggcatggt cgcgctcgtt tccggctctg tcctgcttcg gctgctgcgc gccctgcgcc
     4441 ggccgcagga aacgaccgcc ggtgacgacg agttcgccgg gaccgtatcg gcgatggtgc
     4501 cggccagcct tgcggcactg gctctggcga tgatcattac ggcgttgctc ggctatgtcg
     4561 cgctggccag cctgctggtt gaccaactgc tgtggggcat ggcgatcgcc gccctgatcg
     4621 ggcttgtgct gatcgtggtc gatgaggtgc tggcccgggg gcgcctctcg gaatcccgtc
     4681 cggcacgcca gctccgggtg ctcacaggca ttgacattgg ctcgatgcgg caggccgcca
     4741 ccctgtcctc gggagtagtc aaactgctgc tctacatatt ggccgtgctg atgtttctgg
     4801 ccccgctggg tgtcaattca gatgacatgc tgggctcgtt gcgcgcggcc ttcttcggtt
     4861 tccagcttgg cggcgtcacg atctccatct cgactctggt gctggctctg tcgatcttcg
     4921 tcctggccgt tttcgtcacg cgctcgatcc gtcgctggct ggagggcacc ttcctgccaa
     4981 ccacctcgct cgataccggc ctgcaaaact cgatcctgac ggttcttggc tacatcggct
     5041 tcatcatcgg cgccgttgcg gcgatgggca cgctcgggct tggtttcgac aagatcacca
     5101 ttgttgccgg cgcgctttcg gttggtatcg gctttggcct gcagtcgatc gtcaacaatt
     5161 tcgtctccgg cctgatcctg ctgtgggagc ggccgatccg cgtcggtgac tggatcgttg
     5221 tcggcgagga acagggcaag gtgcagcgca tcaatgtccg cgccacccag atcgagacct
     5281 tcgacaaggc cagcctgatc gttcccaaca gcgaattcat ttccggtcgc gtgaaaaatt
     5341 tcatgcatgc caaccggacg acgcgcatca tcattcctgt cggcgtctcc tacgacagca
     5401 accccgagac cgtgcgccat ctgctgctcg acgctgccat ggcccatcgc gaggtgatgt
     5461 ccgatccggc ccccaacgtg ctgttcatct cgctcggcga atcgagcctc aatttcgaat
     5521 tgcgctgctt tgtcgatgtc gatgcgatgg ccaccacccg atcggacctg atgttcgcga
     5581 tcttcgaggc tttgcggcgc gagaagatca ccgtgccgtt cccgacccgc accgtggaga
     5641 tcgccgacat gaatggtctt gcaggcgcca tcgccgcacg gatgccgaaa aagggctgat
     5701 gttcagccct tctgcaaccg gtcacgcttt ctcagctccg ggaaaagcac gcaccacagc
     5761 gcggtggcca tcaggctgag acagccgccg acgatcgtcg ccggcacgac gccgatccag
     5821 gcggcgacca gtccggattc aaaatcgcca agcgaattgg acgcgccaat gaacagcgaa
     5881 ttcaccgacc cgacgcggcc gcgcatgtca tccggcgtct cggcctgcac aagcgtgccg
     5941 cgcaccacga ccgaaaccat gtcggcagcg ccttcgatca tcatcatgag catcgacagc
     6001 cacagaaggc gcgacaggcc gaaaccgatg atcgccaggc cgaataccgc cgaggcagtc
     6061 agcatccagc gcccgccgtg gcgtcggatc ggatagcggg cgagaaacag cgccatcccg
     6121 agcgctcctg ccgcctggct ggccctcagc cagccaagcg ctgccgggcc ggcctcgaga
     6181 atgtcggtgg catagatcgg cagcagcgcc accaccccgg caaacagaac cgccacaagg
     6241 tcgagcgaga gcgcgcccag cagcaccttg ttggcggtga tgaaccgcgc gccagtggta
     6301 aaggcactga agctgaaggc ttcgcgccgg gccggactgc gctgcggcgt gatggtcaac
     6361 gccgctgcca cgccgagaag attgcacaga caggcaaccg caaatgtcgc gagcggtccg
     6421 aggaggtaga gaaaaccgcc ggccatcggc cccagaatgg tcgccgcctg ccggatcgac
     6481 gcgttccatg ccacagcgct ggcctgccag ccgggccgca ccagattggg cagcatcgcc
     6541 tggttggccg gcagcacgaa ggcctgcgtc gttcccatga gcgtgatcat cgcatagacc
     6601 ggcgcggtcg cggtcagccc ccagccggcg atcagcatca acccgacgcc ggatactgcc
     6661 gccagggcat atcccgccgc cacgatcctg cgccggtcaa aactgtcggc ggcatgcccg
     6721 ctcagcgcca cgaacatgag ctgcggcaaa aaagtgaaca gccccgcaag gccgagtgcc
     6781 agcggatcac gcgtgacctg atagacataa aggccgacac ctacggtctg gatgttctgc
     6841 gccatgccgg acatcaaccg ggccagcgcg aaggcgcgga aatccggctc ggcgaacacc
     6901 gcccagccgc cgcttggctc tcctgtcttg ccagaatcct tcatgccccc gccaccacat
     6961 ccggcgggac cagcaaagca agggtgattg gcaacgggat cattcggaac gcatgctgag
     7021 agatcgaaga atcccgactt gccggcgcaa agtcaaccgt ctcattttgc cggcggcaag
     7081 ggcggcgcca tccgcaccag cacgatggag acacgccggt tggccgaaag gcccggatcg
     7141 ccctggaaca gcggctcgct atcggccttt cctgcaacgc tctcgaagcg gtggcgcgga
     7201 atgccgcgcc ccgccagccg ttcacgcacg atcagcgccc gctgcgttga caccgcccag
     7261 ccgcccgctt cctggacatt gagccggctg gacgaggagt ggccggtaat gcggatcgga
     7321 ttggccagct ggctgatctt gtcggtgatc atgtcaagaa tttttgtagc ccgatcattc
     7381 gggcgcgctt cgccttcgcc gaacatggcc cgcccatcct gatcgacaat ctggatatcg
     7441 aaaccctcgg cggtctcctc gatcagcaca ttcttggaaa cttcggcaat ctcggggagg
     7501 tcctgcattg cctgccgcag cgtggcgacg gcctgcgaca gggcccggtc gccactgccg
     7561 ctgagagcgc tggacggggt cggcttttcg gtttggcgtg ggccggtgaa ttcggaggat
     7621 ttgtccggtg tggtgcgctc gacatagcgc atgtgcggac gcaccggcag cccctcgatc
     7681 tcgacgatcc cggacagttt cgattgcttc tgtgtgccga atgcttcccg catggacccg
     7741 gcgacgatct gcagcttctt ctgatcctgc gtggaaaagg cggtcagcat gacgaagaag
     7801 ctcatcagca ggcccatcag atcggcaaag gtgacgaacc agccgtgacc gccgccgtgt
     7861 ccgccaccct tcttgcgcgc catgactgcc ccccgtcagg ccgcttcgac gaagtcgtgc
     7921 ttatggtgct ccggcagata ggagatcagc atttcgcgaa tgaccgccgg gctcttggag
     7981 gcgcgcatct gcaggacgcc gtcgatgatc aggttgcgcg agacatcctc ctcctccagc
     8041 ttgacatgca gcttgtcggc gatcggcagg cagatgaggt tgccggccag cgcaccatag
     8101 agcgtcgcca gcagtgccac ggccatgaac gggccgagct tggacggatc ttccatgttg
     8161 gagaacatct gcaccatgcc aaccagcgtg ccgaccattc cccaggccgg cgcggcatcg
     8221 ccgatggagc gatagatctt ctgcccctcg tcaagatgct ggaagaaggt atcgcgctcc
     8281 tgttcgagcg tgcggcgaat gaagtcggca tcgtagccgt cggccagata gcgaaccgcc
     8341 tgtttgagaa aggaatcctc gacgacaatc tggtccagcg ccgttcctcc acccttgcga
     8401 taggtctcgg cgatctgggt gatgtcctcg atcagctggc gcggtccgcg ggcccgcatc
     8461 ttgaaggcat atttcagccc catcggaaag ccgtgcttca tggctgacag cggaaaccgg
     8521 atcagcgtgc cggcagcgca tcccccgccg atcacgatga tggcgtggac atcgtaaaac
     8581 atgccgaagt tgccgcccat caggatcagg gccgccaccg tcaccatgcc cacgacaagg
     8641 ccgaggatcg tggaaatgtc cattgcacgc accgatatgt tcgattgccc aatactatct
     8701 gtgattgagt tatgccaaac cgtggttaag gcatggttag gaccggcgac agtccgcgcc
     8761 cttgccgaag cgggccggct aagttagcag cggtcagttg tgactgacgg agacgcacca
     8821 tggcctattc cccggtgatg ctggtgatcc tcgacggctg gggtgagcgc gccgaaactg
     8881 cggacaatgc tgtgcggcag gcccgcacgc ccaacttcac ccggctgtgg aatgcccatc
     8941 cgcatgcatt gctcaacacc tccgggcttg atgtcggtct gccgggcggc cagatgggca
     9001 attccgaagt tggtcatctc aacatcggcg ccggccgcgt ggtcatgcag gatctgccgc
     9061 gcatcggcca ggcgattgcc gatggctcgc tcgccgcctc ccccgctgtt gccagattga
     9121 tcgccgcact caaaatgagc ggcggcacct gtcacctgat cggcctgatc tcgaatggtg
     9181 gcgtgcattc acatcaggac catgcattgg cactcgcccg catcgtgacc gcggccggag
     9241 tgccggtggt gctgcatgcg ctgacggacg ggcgcgacac gccgccgcaa tccggtcttg
     9301 ccttcgttcg cgaggtcgag gctgcgttgc cggccggcgc gcggatcggc acaatcatcg
     9361 gccgctatta cgccatggac cgcgacaacc gctgggagcg cgtgtcgaaa gcctacgatg
     9421 ccatggtctc cggcgccggc actgcctttg ccagcgccgg cgccgcgatc gaggcggcct
     9481 atgccagcaa ggtcaccgac gagttcatcc tgccggcgat catcgccggc tatcccggca
     9541 tgggcgatgg cgacgggttg ctcagcttca acttccgcgc cgaccgggtg cgcgagatcc
     9601 tctccgccat ggtcgatccg gccttcaccg gttttgagcg cagcaggatc gtgcgcctgg
     9661 ccgcccgtgt cggcatggcc cagtattcaa ccgcactcga tgcgcatctg gaaaccatct
     9721 ttgctccgca gaacctcgcc aatgtgctcg gcgccgccgt cgctgccgcc ggcaagcggc
     9781 agctgcgcat ggccgagacg gaaaaatacc cgcatgtcac ctactttctc aatggcggcg
     9841 aggaggcggt ctatccgggc gaggaccgca tcatggtgcc ctcacccaag gtcgcgacct
     9901 acgatcttca gccggagatg agtgctccgg aattgaccga caaggcggtc gccgccatcg
     9961 attcgggtac ctatgacctg atcgtgctca atttcgccaa ccccgacatg gtcggccata
    10021 ccggctcgct ccccgccgcg atccgggcgg tcgaaaccgt cgatgccggc cttggccgca
    10081 tcgccgatgc gattgcccgg cagggcggcg cgctgctgtt caccgccgat catggcaatt
    10141 gcgaactgat gaaggacccg gtgaccggcg gcccgcacac ggcgcatacc accaatccgg
    10201 tgccggcatg ccttactgcc ggcgcccgcc gcctgcgcga cggcggccgg cttgccgatc
    10261 tcgccccgac cctgctccaa ctgatgggca tcgcccagcc gaaggagatg aacggtcgct
    10321 cgctgattgc cgagtgacgc cggcgccccg gctggttgcc ggggcgggcg gccctcctac
    10381 ttgctcgcgc tccaggtgcc tttcgaggcg ccccagcggt tgctgcccga ccaggtgccc
    10441 gatccgcttc ggccattgac cttgccgccg atcttgccgg taaaggtgaa ggttgaggtc
    10501 tttccatcag cccaggtggt gcgcagcaca ccattggcgg cagccgagaa ggtgccttcc
    10561 gccgaaagcg ggcccgagaa gctcgcattg atcggatcgc cttcatgcac accgccgatc
    10621 gtgccgctga cctgcccacc cgagacggta aaggtcagcg atccggagga gccgccggcg
    10681 atccgccccg aataggtgcc ttcaagcgcc ggattgaccg gtgcgctggc ggtctgtggc
    10741 tgtgcaccac cgctttcggt gaaatgctgg cgcccggttt ccttgccgct ggcgtcgtag
    10801 ttgacgtaat agccgccgat ccggttgccg ttggcgtcgt tgatatagac gaccttggtc
    10861 atcccgctgc tgttcttcac ccagccgccc tcgacctcgg tagtcgtc
//
LOCUS       DAOMXG010000074        10807 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_3253_length_10807_cov_9.927083, whole genome shotgun sequence.
ACCESSION   DAOMXG010000074 DAOMXG010000000
VERSION     DAOMXG010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 10807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10807
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3253_length_10807_cov_9.927083"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1752
                     /gene="thrS"
                     /locus_tag="PLQ11_06580"
     CDS             <1..1752
                     /gene="thrS"
                     /locus_tag="PLQ11_06580"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HRK24601.1"
                     /translation="AHVLAEAVQPLWPGTQVTIGPVIENGFYYDFHRNEPFTPEDFAA
                     IEKKMREIIARDSAFTKEEWSRDQAKEFFRAKGEAFKVELVDAIPPGESLKIYKQGEW
                     LDLCRGPHMTSVGKIGNAFKLMKVAGAYWRGDSTRPMLTRIYGTAFARQEDLDAHLKQ
                     IEEAEKRDHRKLGREMDLFHFQEEGPGVVFWHSKGWRMFQQLTAYMRRRLEADYEEVN
                     APQILDKSLWETSGHWGWYADSMFAVKSASALIRPNDPEADQRVFALKPMNCPGHVQI
                     FKHGLKSYRDLPIRLAEFGNVHRYEASGALHGLMRVRGFTQDDAHIFCTDAQLEEECL
                     KINELMLSVYADFGFTELVVKLSTRPEKRVGSDDLWDRAETIMRAVLDKIAAQSGGKI
                     RTAINEGEGAFYGPKFEYTLRDAIGRDWQCGTTQVDFNLPERFGAYYIDSDGEKRQPV
                     MIHRAICGSMERFLGILIESYAGHFPLWLSPEQVLVCTITQEADAYAETVVGQAFKAG
                     LRARADLRNEKIGYKVREHSLAKVPVIFAVGKREAEEGTVAIRRLGSQAQVVMKLDAA
                     LAMLAAEATPPDVQRAG"
     gene            complement(1759..2826)
                     /locus_tag="PLQ11_06585"
     CDS             complement(1759..2826)
                     /locus_tag="PLQ11_06585"
                     /inference="COORDINATES: protein motif:HMM:NF023695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3616 domain-containing protein"
                     /protein_id="HRK24602.1"
                     /translation="MEPVLVEIVMRRIIGALLVTMSLAPPLAAQTGLVRYSEICDASG
                     AVALDGETFLVVSDEIDRAKGEKKQNTGDTLVVYRRGTPAPIQKFPLKDFLPDASGDN
                     DEADLEGVARIGNTLYFIGSHARSRAGKGALFAPNRHVLFSAKLEPDAGKHKITPDGD
                     APYRGLLADILASSDVAENLRVASTLAPDAPGGFNIEGLAATPGNGLFIGLRSPLIDG
                     KALVLSLEQPDKVLTGAPASFGATARLDLGRRGIRALHWTGNEVLIVAGPVGEGKDPS
                     FGTDFALFRWSGKAGEAATEIKNVAVNSLRPEAVFIFPGSKQGMLLSDDGTEQVKDKD
                     CKNKETARPDRSFRALPFTLD"
     gene            2996..3949
                     /locus_tag="PLQ11_06590"
     CDS             2996..3949
                     /locus_tag="PLQ11_06590"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HRK24603.1"
                     /translation="MSTTPSNSRAFPHRHLLGIAGLHRLDIEALLDAAEAEVATSRQV
                     EKKKATLRGRTQINLFFEASTRTQASFELAGKRLGADVMNMSVANSSVKKGETLIDTA
                     ITLNAMRPDLIVVRHHAAGAVQLLARKVDCSVINAGDGAHEHPTQALLDALTIRRNKG
                     RIEGLVVAICGDIAHSRVARSNIICLAALGARVRCVGPTTLLPKGLDRMGVEVFTDMK
                     KGLKDADIVMMLRLQRERMNGSFVPSMREYFHFFGLDEEKLAFAKPDCLVMHPGPMNR
                     GVEISSAVADGAQSLIREQVEMGVAVRMAVLEALSRRLPNA"
     gene            3949..4218
                     /locus_tag="PLQ11_06595"
     CDS             3949..4218
                     /locus_tag="PLQ11_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24604.1"
                     /translation="MRDDDDDSDRRPYNAELAVLLQGALRARARVLALFLGLAVVTFV
                     WFAKDAGLPLWLVLAIGVLGAVLIAFLVALVWFWLRFLSHFRGRG"
     gene            4218..5522
                     /locus_tag="PLQ11_06600"
     CDS             4218..5522
                     /locus_tag="PLQ11_06600"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HRK24605.1"
                     /translation="MPDTDRRPLLLTNARLVDPASATEQKGGVLIEGGIIRDLGPGLV
                     ASAVSDETHVHDCHGAVVAPGLVDLRVHVGEPGAEDRETLRTLSQAAAAGGVTTVVTM
                     PDTDPAVDEPAIVDFVLRRARATASIRVHPAAALTKGLAGKEMTEIGLLLEAGAVAFT
                     DATRSVMNAQVLRRALGYGSMFNALVMQHLEEPTLVGEGVVSEGEFASRMGLPGVPRE
                     AETIVLERDMRLVRMTGGRYHASVVTCPDSLDVLARAKAAGLPVTAGTSINHLSLNEN
                     DIGEYRTFLKLAPPLRTEDERLALVAALADGLIDVIVSDHNPQDVEAKRQPFVECANG
                     AVGVETMLAAGLRLVHSGHVSLTRLLNAMSTRPAELIGVPGGRLERGAPGDVIVFDPD
                     MPWVVDAEKLHSRCRNTPFDEARMSGKVLMTIVAGRVVYEAG"
     gene            5587..6168
                     /gene="plsY"
                     /locus_tag="PLQ11_06605"
     CDS             5587..6168
                     /gene="plsY"
                     /locus_tag="PLQ11_06605"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003599346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HRK24606.1"
                     /translation="MTSLLALGLGYLLGAIPFGLLLTRMAGTEDIRTIGSGNIGATNV
                     LRTGSKGLALATLLCDALKGTAAVMIARHVWGGDEGLLAGLAAFLGHLFPVWLGFKGG
                     KGVATYLGVLLAVSSAAFVVAGLVWLAVAYVTRYSSAGSLTTSAITPLAVWMLATAPA
                     ALLFLVMAPLLWWKHSENIQRLRAGTESKIGKT"
     gene            6165..7406
                     /gene="dprA"
                     /locus_tag="PLQ11_06610"
     CDS             6165..7406
                     /gene="dprA"
                     /locus_tag="PLQ11_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003610886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRK24607.1"
                     /translation="MNGWPAPAPTGIRLSDQQRLDWLRLSRSEGVGPMTFTALINRFG
                     GAGAAVEALPELLRAKGRSPAGIADLASVEREIEAATRLGARFIARGEADYPALLREI
                     ADAPPLVAVLGRIELLHLPLVAMVGSRNASGVGRSFAERMAHDLTRHGYGVVSGLARG
                     IDTRVHVAALAFGTVAVLAGGLDRPYPPENLDLMRRIGESGAVISEMPFGWEPRGRDF
                     PRRNRIISGLCMATVVVEAARKSGSLITARFALEQGREVFAVPGSPLDPRAEGSNDLI
                     KASKAHFCTSVEDIIAELQPMLRAPDLFGEPARATSAATYQGAPLWDELFGDEQEGTT
                     PGLFGEAEAIPADFEPPGDLAARILALLGPSPLAIDDLLRLAEAPAPAVHAILTDLDL
                     AGRLERHGGNRVSLVVSACRD"
     gene            complement(7501..8421)
                     /locus_tag="PLQ11_06615"
     CDS             complement(7501..8421)
                     /locus_tag="PLQ11_06615"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HRK24608.1"
                     /translation="MPPIVETVLTVLIVTALGWALTRWSLIRQDHWEGFEHIAYQVFF
                     PALVLHALIKADLSAVPSLAFGITLFLTIVVAAALLVLLARPIMRITGTDGPGFTSLF
                     QGTIRWNTFMAFGLIESLYGARGLTLMAVAIAAIIPLVNVVSVLVLRRFAGGAGSFNP
                     LYLLRNPFIWSSLLGLAINLSGLPVPNVLVDAAGMCGKASLAGALLLVGSGLKLGDLE
                     RPNAAILLSSGLKLLGLPLLAAALAWPFGIRGADLGVLVLCAAVPTASAGYILARQMG
                     GDARLMAAIITVQTLVSALTLPAMLLLLAR"
     assembly_gap    8662..8671
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8672..9189
                     /locus_tag="PLQ11_06620"
     CDS             <8672..9189
                     /locus_tag="PLQ11_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24609.1"
                     /translation="QPAAAGPIPISRSDGAAPLVEQVQYRRRPVVVRKKRRGDGAAAA
                     ALAIGALAIGAAVMANQSQRRDRAYYYEDDPYYHPGYGYRTYQPQPYYAPQVQPRYRQ
                     RYVPQYQAPEYYAPQPRAHRWRPPAQPDAGSNYAIEQQRAQNRGYRGQPRVYVNPAPP
                     VPPQPKPPEAW"
     gene            complement(9162..9662)
                     /gene="ruvX"
                     /locus_tag="PLQ11_06625"
     CDS             complement(9162..9662)
                     /gene="ruvX"
                     /locus_tag="PLQ11_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HRK24610.1"
                     /translation="MAGAVIPLEELAPRLERGGRLLGLDLGSKTIGLALSDVEYRIAT
                     ALRTLERGKFRDDAAALARIVDEFGIVALVLGLPLNMDGSSGPRVQATRAFARNLLPL
                     LPLPIVLWDERLSTAAAERVLISADLSRTKRAAVIDATAAAYILQGALDRLARIDPHQ
                     ASGGLG"
     gene            9732..10487
                     /locus_tag="PLQ11_06630"
     CDS             9732..10487
                     /locus_tag="PLQ11_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008197240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HRK24611.1"
                     /translation="MKLTWYGHSAFALEAAGKKLLIDPFLTGNPSFVGPGDSAATASA
                     YAAAIAGTSHVVVTHGHGDHVGDAADICIRTRPTLFCNYDLGVWLMGKVAAANGGQES
                     GASFELMNTGGTVENGGVSVTLVRADHSSGMQEGEMAQALGMPNGAIVRLPGAPIVYH
                     MGDTDIFSDMGLINEIWKPDVVIIPIGDRFTMGPRTAALAIRRYFPGVRAVVPCHWGT
                     FGLLSGTPAMLKAELGDLAGRVVDLAPGGSVAL"
     gene            10623..>10807
                     /locus_tag="PLQ11_06635"
     CDS             10623..>10807
                     /locus_tag="PLQ11_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24612.1"
                     /translation="MTARDPVRVAAEAGALDIVVDPALIAPEGWRCLDLTRLGGVTIG
                     GARISARFAAMPAVAGGA"
BASE COUNT         1831 a         3331 c         3740 g         1895 t
ORIGIN      
        1 gcccatgtgc tggccgaagc ggtgcagccc ctgtggccgg gcacgcaggt gaccatcggc
       61 ccggtgatcg agaacggctt ctattacgac ttccaccgca acgagccgtt cacgcccgag
      121 gattttgccg ccatcgagaa gaagatgcgc gagatcatcg cgcgcgattc cgcctttacc
      181 aaggaagagt ggagccgcga tcaggccaag gagttcttcc gggccaaggg cgaggcgttc
      241 aaggtcgagc tggtcgatgc cattccgccg ggtgaatcgc tgaagatcta caagcagggc
      301 gaatggctcg atctctgccg cggcccgcac atgacctcgg tcggcaagat cggcaatgcc
      361 ttcaagctga tgaaggtggc cggcgcctac tggcgcggcg attccaccag gccgatgctg
      421 acgcgcatct atggcaccgc ttttgccagg caggaggacc ttgatgcgca cctgaagcag
      481 attgaggagg cggaaaagcg cgaccaccgc aagctcggtc gcgagatgga cctgttccat
      541 ttccaggagg aggggccggg cgtcgtgttc tggcactcca agggctggcg catgttccag
      601 cagcttaccg cctatatgcg gcggcggctg gaggccgatt acgaggaggt caacgcgccg
      661 cagatcctcg acaagagcct gtgggagacg tccggccact ggggctggta tgcggattcg
      721 atgttcgcgg tgaaatcagc ctcggcgctg atccgtccca acgatccgga ggccgaccag
      781 cgggtgttcg cgctgaagcc gatgaactgc cccggccatg tgcagatctt caagcatggg
      841 ctcaagagct accgcgacct gccgatccgg ctggcggaat tcggcaatgt ccaccgctac
      901 gaagcctcgg gtgcgctgca cgggctgatg cgcgtgcgcg gcttcacaca ggacgatgcg
      961 catatcttct gcacggacgc gcagcttgag gaagagtgcc ttaaaatcaa tgaattgatg
     1021 ctttctgttt atgcggattt cggcttcacc gagctggtag tgaagctgtc gacccggccg
     1081 gaaaagcgtg tcggctctga cgatctgtgg gatcgcgccg agacgatcat gcgcgccgtg
     1141 ctcgacaaga tcgcggccca gtcgggcggc aagatcagaa ccgcgatcaa cgagggtgag
     1201 ggcgccttct acggtccgaa attcgaatat accctgcgtg acgctatcgg ccgcgactgg
     1261 caatgcggca ccacgcaggt cgatttcaac ctgccggagc ggttcggcgc ctactacatc
     1321 gactccgatg gcgaaaagcg ccagccggtg atgatccatc gcgccatctg cggctcgatg
     1381 gagcggtttc tgggcatcct gatcgagagc tatgccgggc atttcccgct gtggctgtcg
     1441 ccggagcagg tgctcgtctg cacgatcacg caggaggccg atgcctatgc cgaaaccgtg
     1501 gtggggcagg cgttcaaggc tggattgcgc gcccgcgccg atctccgcaa cgagaagatc
     1561 ggctacaagg tgcgcgagca ttcgctggcc aaggtgccgg tgatcttcgc tgtcggcaag
     1621 cgcgaggcgg aagagggcac ggtggccatc cgccggctcg gcagccaagc gcaggtggtg
     1681 atgaagctcg acgctgcgtt ggccatgctg gcggcggaag caaccccgcc ggatgttcag
     1741 cgcgcagggt gaggctgctc aatcgagggt gaagggcagg gcccggaacg agcgatcggg
     1801 ccgggccgtc tccttgttct tgcaatcttt gtccttgacc tgctcggtac cgtcgtcgct
     1861 gagcaacatt ccctgcttgc tgccgggaaa gatgaacacg gcttccggcc gcaggctgtt
     1921 gaccgccacg ttcttgattt ccgtggcggc ctccccggcc ttgccgctcc agcggaacag
     1981 ggcaaaatcg gtgccgaaag acgggtcctt gccctcgccg accggaccgg cgacaatcag
     2041 cacctcgttg ccggtccagt gcagggcacg aatgccgcgc cggccgaggt caagccgggc
     2101 ggttgcgccg aagctggccg gagcgcctgt cagcaccttg tccggctgct caagagacag
     2161 caccagtgcc ttgccgtcga tcagcggact gcgcaggccg atgaaaagcc cgttgcccgg
     2221 cgttgcggcg agcccttcga tgttgaatcc cccgggcgcg tcgggagcca gcgtgctggc
     2281 cacgcgcaga ttttcggcga catcgctgga ggccagaata tcggccaaca ggccccggta
     2341 gggagcatcg ccatcagggg tgatcttgtg cttgccggcg tcgggttcca gctttgcaga
     2401 gaacagaacg tgacggttgg gagcaaaaag cgcgcccttt ccggcgcggc ttcgcgcgtg
     2461 agagccgatg aaatagagcg tgttgccgat gcgggcgacg ccttccagat cggcctcgtc
     2521 gttgtcgccg gaggcatcag gcagaaagtc cttgagaggg aatttctgga tcggtgccgg
     2581 tgttccgcgc ctgtaaacga ccagtgtgtc gccggtgttc tgcttttttt cgcccttggc
     2641 ccggtcgatc tcgtcgctga cgacgaggaa ggtttcgcca tccagtgcca ccgcgccgga
     2701 ggcatcgcag atttcgctgt aacggaccag gccggtctgg gcagcgagtg gcggggcaag
     2761 ggacattgtg acaagcaggg cgccaatgat gcgtcgcatg acgatctcca cgagaactgg
     2821 ttccatcaga gcacaaatcc cgcatgcccc gcgtcaccca cttgcatgaa ccttgaccgc
     2881 cggaacaagg gcgagtgcca gcgccctgaa tgtccacgat ctcaactata acggttgaga
     2941 acccaacgtg gcgcttgccg gggcgggcgg gagagcgtag agcctcgtct tcgacatgag
     3001 caccacgccg tcgaattccc gcgcctttcc gcaccgccat ctgctcggca ttgccgggct
     3061 gcatcggctt gacatcgagg cgctgctcga tgctgccgag gccgaggtag caacctcccg
     3121 ccaggtcgag aagaagaagg ccactttgcg cggccggacg cagatcaacc tgttcttcga
     3181 agcctccacc cgcacccagg cgagtttcga actggccggc aagcggctcg gcgccgatgt
     3241 gatgaacatg tcggtggcca attcctcggt gaagaagggc gagacgctga tcgacacggc
     3301 gatcacgctc aacgccatgc ggcctgacct gatcgtggtg cggcatcatg cggcgggggc
     3361 ggtgcaactg ctcgcccgca aggtcgattg ctcggtgatc aacgccggcg acggagcgca
     3421 cgaacatccg acacaggcgc tgctcgatgc gctgaccatc cgccgcaaca agggccgcat
     3481 cgaggggctg gtcgtcgcga tctgcggcga catcgcccat tcgcgcgtcg cccgctctaa
     3541 catcatctgc cttgcggcgc tgggcgcgcg ggtgcgctgc gtcgggccga cgacgctgct
     3601 gcccaagggc ctcgaccgca tgggcgtcga ggtgttcacc gacatgaaga aggggctgaa
     3661 ggacgcggac atcgtgatga tgctgcggct ccagcgcgag cggatgaacg gctcgttcgt
     3721 gccgtcgatg cgcgagtatt tccacttttt cggcctcgat gaggaaaagc tcgccttcgc
     3781 caagcctgac tgtctggtca tgcatcccgg cccgatgaac cgcggcgtcg agatttcctc
     3841 cgccgtggcg gacggggcgc agtcgctgat ccgcgagcag gtggagatgg gggtggccgt
     3901 gcgcatggcg gtgctggagg cgctgtcccg ccggctgccg aatgcgtgat gcgcgacgat
     3961 gacgatgaca gtgaccggcg cccctacaac gccgagcttg ccgtcctgct tcagggcgca
     4021 ctccgcgcca gggcgcgtgt ccttgcgctg tttcttgggc tggctgtcgt caccttcgtc
     4081 tggttcgcca aggatgcggg gctgccgctc tggctggttc tggccatcgg cgttctcggc
     4141 gcggtgctca tcgccttcct tgtcgcgctc gtctggttct ggctgcggtt cctcagccac
     4201 ttcaggggga ggggctgatg cccgatactg accgccgccc gttgctgctg accaacgccc
     4261 gtctggttga tccggcgtcc gcaaccgaac agaagggcgg cgtgctgatc gagggcggca
     4321 tcatccgcga tctcgggccg ggtctggtcg ccagcgctgt cagcgacgaa acccatgtgc
     4381 atgattgcca cggagccgtg gtggcgccgg ggctggtgga cctgcgcgtg catgtcggtg
     4441 aaccgggcgc cgaggatcgc gagacactgc gcaccctcag ccaggccgcc gctgccggag
     4501 gggtgaccac ggttgtcacc atgccggaca ccgatccggc ggtcgacgaa ccggcgatcg
     4561 tcgatttcgt gctgcggcgg gcgcgggcca ccgcgtcgat ccgcgtgcat ccggcggctg
     4621 cgctgaccaa gggcctggcc ggaaaggaaa tgaccgagat cggcctgctg ctcgaagcgg
     4681 gggcggtggc gttcaccgat gcgacgcgct cggtgatgaa cgcgcaggtg ctgcggcgcg
     4741 cgctcggcta cgggagcatg ttcaacgcgc tggtgatgca gcatctcgaa gagccgacgc
     4801 tggtcggcga aggggtggtg agcgaaggcg aatttgcctc gcgcatgggg ctgccaggtg
     4861 tgccgcgcga ggccgagacc atcgtgctcg aacgcgacat gcggctggtg cgcatgaccg
     4921 gggggcgcta tcacgcctcg gtcgtcacct gtccggattc gctcgatgtg ctggcccgcg
     4981 ccaaggcggc cggtctgccg gtcacggcag gcacctcgat caaccatctg tcgctcaacg
     5041 agaatgacat cggcgaatat cgcaccttcc tcaagctcgc cccgccgctg cgcaccgagg
     5101 atgagcgcct ggccctggtt gcggcgctgg cggacgggct gatcgacgtc atcgtctccg
     5161 atcacaatcc gcaggatgtg gaggccaagc gccagccgtt tgtcgaatgc gccaacgggg
     5221 cggtcggggt cgagacgatg ctcgcggcgg ggctcaggct ggtgcattcc ggccatgtga
     5281 gcctgacccg gctgctcaat gccatgtcga cgcggccggc ggagctgatc ggcgtgcccg
     5341 gcggacggct ggagcgcggc gcgccgggcg atgtgatcgt gttcgatccg gacatgccct
     5401 gggtggtcga tgcggaaaag ctgcattcgc gctgccgcaa cacgccgttc gacgaggcgc
     5461 ggatgagcgg caaggtgctg atgacgatcg tcgcggggcg ggtggtctac gaagcgggct
     5521 gattgcgttt cctgcccgga tggccttgaa tggcgccgtg ttggatcatg cggatgtgag
     5581 gcggaaatga cgagcctgct tgcccttggc cttggctatc tgcttggcgc tatcccgttc
     5641 ggcctgctcc tgacacggat ggccggcacc gaggatatcc gcaccatcgg ctcgggcaat
     5701 atcggcgcca ccaatgtgct gcgcaccggc agcaaggggc tggcgctggc gacgcttctc
     5761 tgtgacgcac tgaagggcac ggcggcggtg atgatcgcgc gccatgtctg gggcggcgac
     5821 gaagggctgc tggccgggct ggcggcgttt ctcggccatc tgtttccggt gtggctcggc
     5881 ttcaagggcg gcaagggcgt tgcgacctat ctcggcgtgc tgctggcggt gtcgtcggcg
     5941 gccttcgtag tcgccgggct ggtctggctc gcggtggcct atgtcacccg ctattcctcg
     6001 gccggttcgc tgacaacgag cgcgatcacc ccgctggccg tctggatgct cgctaccgcg
     6061 ccggccgctc tgctgtttct ggtgatggcg ccgctgttgt ggtggaagca ctcggagaac
     6121 atccagcgat tgcgcgccgg caccgagagc aagatcggga agacatgaac ggctggccgg
     6181 cgccggcgcc aaccgggatt cgtctgtccg atcagcagcg gctcgactgg ctgcggctgt
     6241 cgcgcagcga aggcgtgggg ccgatgacct tcaccgcgct gatcaaccgt ttcggcgggg
     6301 ccggggcggc ggttgaggca ctgccggaac tgctgcgggc caaggggcgc agcccggcgg
     6361 ggatcgctga ccttgccagt gtcgagcgtg agattgaggc cgcgacacgg cttggcgcgc
     6421 gattcatcgc gcggggcgag gctgactatc cggcgctgtt gagggaaatt gccgatgcgc
     6481 cgccgctggt cgcggttctc ggccgcatcg aactcctgca cctgccgctg gtggcgatgg
     6541 tgggctcgcg caatgcgtcg ggcgtcgggc gcagctttgc cgagcggatg gcccatgatc
     6601 tgacccggca cggctatggc gtcgtctccg gccttgcccg gggcattgat acgcgtgtgc
     6661 atgtcgcggc gctggcattc ggcactgtgg ccgtgcttgc cggcggcctt gaccggccct
     6721 atccgccgga gaacctcgat ctcatgcgca ggattggcga gagcggcgcc gtgatcagcg
     6781 agatgccgtt cggctgggag ccgcgcgggc gtgattttcc gcggcgcaac cggatcattt
     6841 ccggcctgtg catggcgacc gtggtggttg aggcagcgcg caagtcggga tcgctgatca
     6901 ccgcccgctt tgctctcgaa caggggcggg aggtatttgc cgtgccgggc tcgccgctcg
     6961 atccgcgcgc cgagggcagc aatgatctga tcaaggccag caaggcgcat ttctgcacat
     7021 cggtcgagga tatcatcgcc gaattgcagc ccatgctgcg ggcgccggac ctgtttggcg
     7081 agccggcccg cgccaccagc gccgcgacct accagggtgc tccgctgtgg gatgaactgt
     7141 tcggcgacga acaggagggc acgacgccgg ggctgttcgg cgaggcagag gctattcctg
     7201 ccgattttga gcctcccggc gatcttgccg cccgcatcct cgccctgctg gggcccagcc
     7261 cgctggccat cgacgatctg ctgcgcctgg ccgaggcgcc ggcaccggcc gtccatgcga
     7321 tcctgaccga ccttgatctg gccggacggc ttgagcgcca cggcggcaac cgggtgtcat
     7381 tggtggttag tgcatgtcgc gattaggtgg ataccggtca atcgcagaag acatgctcta
     7441 acttattgat accgatcacg ttatccgcat tttgatgatt ccatcaaaat gcggcgtgat
     7501 ctagcgcgcc agaagcaaca gcattgccgg cagggtgagc gccgagacca gagtctggac
     7561 ggtgatgatc gctgccatga gccgcgcatc gccgcccatc tggcgggcca ggatatagcc
     7621 ggctgaggcg gtcggcaccg ccgcacacag gaccagaacg ccgagatcgg cgccgcgaat
     7681 gccgaacggc caggccaggg ccgcagccag cagcggcagg ccaagcagct tgaggccgga
     7741 actcagcagg atcgcggcgt tcggccgttc aagatcgccc agtttcagcc cggagccgac
     7801 cagcagcagg gcgccggcca gcgatgcctt gccgcacatg ccggcggcat caaccagcac
     7861 gttggggaca ggcaggccgg aaagattgat cgccagcccg agcagcgaag accagatgaa
     7921 cggattgcgc aacaggtaaa gcgggttgaa gctgccggct ccgccggcaa aacggcgcag
     7981 cacaagcaca gaaacaacgt tgaccagcgg aatgatggca gcaatcgcca ccgccatcag
     8041 cgtcaggccg cgcgcgccat agaggctttc gatcaggccg aaggccatga aggtgttcca
     8101 gcggatcgtg ccctgaaaca gcgaggtgaa gcccggcccg tcggtgccag tgatccgcat
     8161 gatcggccgg gccagcagca ccagcagcgc ggctgccaca acaatggtca ggaacagcgt
     8221 tatcccgaag gccagcgaag gaaccgcaga aaggtctgcc ttgatgagcg cgtgcagcac
     8281 cagcgccgga aaaaacacct gataggcgat gtgttcaaag ccttcccaat gatcctggcg
     8341 gatcagcgac cagcgcgtca gggcccagcc aagcgccgta acaatcagga cagtgaggac
     8401 ggtttcgaca atggggggca tgaaagggtc cggctgcgga gcgggggcag ggtgtcttgg
     8461 tggcacgaat cctcgccggc agaaagccgg gccggtaagg ttgccagcaa ttattcagtg
     8521 cgggttcatg gccgctgcgt taagaaagtg ttgacgatca gcggcgttgt gcccgccggt
     8581 cgacaaaggg agacgacagg atgtcgcgtt ctaagggcct tcgccgcatt gctgcgggtg
     8641 ttctttgcgg gtcgctgctg gnnnnnnnnn ntccagccgg ctgccgctgg gcccatcccg
     8701 atcagccgtt ccgatggcgc cgccccgctg gttgagcagg tgcaatatcg ccgtcggccg
     8761 gtcgtcgtgc gcaagaagcg gcgcggcgat ggcgctgcgg ctgcggcgct ggccatcggt
     8821 gcgctggcga tcggcgcggc ggtgatggcc aatcagtcgc agcggcgcga tcgtgcctat
     8881 tactacgagg acgatcctta ctatcatccc ggctacggct atcgcaccta tcagccgcag
     8941 ccgtattacg cgccgcaggt tcaaccgcgg tatcggcagc ggtatgtgcc gcagtatcag
     9001 gcgccggaat attatgcgcc gcagccgagg gcgcatcgct ggcgtccgcc ggcgcagccc
     9061 gatgccggct cgaattatgc gatcgagcag cagcgtgcgc agaaccgcgg ctatcgcggc
     9121 cagccgcgtg tctatgtcaa tccggcgccg ccggtgccgc ctcagcccaa gccgcccgag
     9181 gcctggtgag gatcgatacg ggcgagccgg tcgagcgcgc cctgcagaat ataggcggcg
     9241 gcggtggcat cgatcaccgc cgcccttttt gtccgtgaca gatcggcaga gatcagcacc
     9301 cgctcggcag cggcggtgga gaggcgctcg tcccacagca cgatgggcaa gggcaggagc
     9361 ggcagaaggt tgcgcgcgaa ggcgcgggtt gcctgcacgc gcgggccgct cgacccgtcc
     9421 atgttgagcg gcaggccgag aaccagcgcg acgatgccga attcgtcgac aatccgcgcc
     9481 agcgccgccg catcgtcgcg aaatttgccg cgttccagtg tgcgcagcgc ggtggcaatg
     9541 cggtattcga catccgacag ggcaaggcca atcgtcttgc tgcccagatc aagaccaagc
     9601 aggcggccac cgcgttcaag gcgcggggct agttcttcca gggggatgac ggcgccagcc
     9661 acgctttgct cctgacattg cacgccctgc ctgatactcc tctaaagtga ttggcccaag
     9721 ggagagttta catgaaactg acgtggtatg gtcattccgc cttcgcgctg gaagcggcgg
     9781 gcaagaagct gctgatcgat ccgttcctca ccggaaaccc cagttttgtc ggcccgggcg
     9841 attcggctgc gacggccagt gcctatgcag cggcaatcgc cgggaccagc catgtcgtgg
     9901 tcacccatgg gcatggcgat catgtcggcg atgcagcgga tatctgcatc cgcaccaggc
     9961 cgacactgtt ctgcaactat gatctcggcg tgtggctgat gggcaaggtc gcggcggcca
    10021 atggcggtca ggagtcgggc gcctcgttcg agttgatgaa caccggcggc acggtggaga
    10081 acggcggggt ctcggtgacg ctggtgcgtg ccgaccattc ctccggcatg caggaaggcg
    10141 agatggcaca ggcactcggg atgccgaacg gcgccatcgt gcggctgccc ggtgcgccga
    10201 tcgtctatca catgggcgat accgacattt tctcggatat ggggctgatc aacgaaatct
    10261 ggaagcccga tgtggtgatc attccgatcg gcgaccgttt caccatgggg ccgcgcacgg
    10321 cggcgctggc gatccgccgc tatttccccg gtgtccgggc tgtcgtgccc tgccattggg
    10381 gcaccttcgg cctgctcagc gggacgccgg ccatgctgaa ggcagaactg ggtgatcttg
    10441 ccggccgggt ggtcgatctg gcgccaggcg gcagcgtcgc gttgtagagg atccggcggc
    10501 gcgatgtatc ggaatgatac gcggcgccga agctgccatt aaccctctgt tgacaataaa
    10561 atggccgccg acggggggct ttgctagcgt ctgccggttg aacaagccgg cggagctgac
    10621 ccatgaccgc gcgtgatcct gttcgcgttg ccgccgaggc gggggcgctt gatattgtcg
    10681 tcgatccggc gctgatcgcg cccgagggct ggcgttgcct tgatctgacc cggctggggg
    10741 gcgtgacgat cggcggcgcc cggatttcgg cacggtttgc cgccatgccg gcggtcgcag
    10801 gcggggc
//
LOCUS       DAOMXG010000075        10730 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_3278_length_10730_cov_10.285246, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000075 DAOMXG010000000
VERSION     DAOMXG010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 10730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10730
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3278_length_10730_cov_10.285246"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2726)
                     /locus_tag="PLQ11_06640"
     CDS             complement(<1..2726)
                     /locus_tag="PLQ11_06640"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HRK24613.1"
                     /translation="MDRDLDLSALSGSNAAYLEALTGDVTPGPSWKRSNWPVVARTEL
                     TSALDSDWSSVEKAVADKLKGKAAAGGAAPSAAQLQAAARDSVHALMMIRAYRMRGHL
                     HADLDPLGIEPRKDHEELHPSSYGFTEEDWDRKIFIDHVLGLEFATIREMLAILRRTY
                     CATIGYEFMHISDPAQKAWLQARIEGPEKEITFTREGKRAILNKLVEAEGFEKFLDRK
                     FTGTKRFGLDGGEALIPGLEQIIKRGGNLGVKEIVFGMAHRGRLNVLTQVMAKPHRAL
                     FHEFKGGSFAPDEVEGSGDVKYHLGASSDREFDGNKVHLSLTPNPSHLEIVNPVVLGR
                     VRAKQDQLNCTPEDRRAVLPLLIHGDAAFAGQGVVAECFGMSGLKGHRTGGWVSVIIN
                     NQIGFTTYPRFSRSSPYPSDMAKIVEAPIFHVNGDDPEAVVFVCKVAAEFRQKFQKPV
                     VVDMWCYRRFGHNEGDEPAFTQPLMYQKIRSHRTTLEIYAEKLIAEKVVTQGEVDKMK
                     ADWVSRLEAEFEAGHAYKPNKADWLEGAWEGLKAGTDAHDDPLHGKTGVAIDTLRSIG
                     RKIATIPEGFNAHRTIQRFMDGRLKMVESGEGLDWAMAEALAYGTLVDEGYPVRLSGQ
                     DCERGTFSQRHSVIIDQVNENRHTPLNNLRAGQKRYEVINSMLSEEAVLGFEYGYSLV
                     EPNTLTLWEGQFGDFANGAQVVFDQFISSGERKWLRLSGLVCLLPHGFEGQGPEHSSA
                     RLERFLQLCAEDNMQVANCTTPANYFHILRRQMKRDIRKPLILMTPKSLLRHPRCKSS
                     LADLGADSSFHRFLRDDADGGNAGYSLAKDAKIRRVIICTGKVYFDLLEEREKRNLDD
                     VYLLRVEQLYPFPLKALTKVMQRFRKADVVWCQEEPKNMGGWSFVEPYLEWVL"
     gene            complement(2747..3088)
                     /locus_tag="PLQ11_06645"
     CDS             complement(2747..3088)
                     /locus_tag="PLQ11_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHCW motif cupin fold protein"
                     /protein_id="HRK24614.1"
                     /translation="MRMTDIPFGTTDWSAVTPTRHPGAEGEAIWRTRTFGPEGNAIRV
                     RLVDYSAGYVSDHWCEKGHILLCLEGELETTLADGRVFVLKPGMSYQVADGAEPHRSR
                     TQSGARLFIVD"
     gene            complement(3092..3979)
                     /gene="sucD"
                     /locus_tag="PLQ11_06650"
     CDS             complement(3092..3979)
                     /gene="sucD"
                     /locus_tag="PLQ11_06650"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003599893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HRK24615.1"
                     /translation="MSILIDKNTRVICQGFTGKNGTFHSEQAIAYGTQMVGGTSPGKG
                     GSMHLGLPVFDTVAEAREATGADASVIYVPPPGAADAICEAIQAEIPLIVCITEGIPV
                     TDMIRVKRALSGSKSRLIGPNCPGVMTAGECKIGIMPGNIFKKGNVGIVSRSGTLTYE
                     AVFQTTQEGLGQTTAVGIGGDPVKGTEFIEMLEMFLKDEATKSIVMIGEIGGSAEEDA
                     AQFIRDEAKRGRKKPMVGFIAGRTAPPGRRMGHAGAIISGGKGGAEDKIAAMEAAGIR
                     VSPSPARLGKTLVDVLKGK"
     gene            complement(3996..4352)
                     /locus_tag="PLQ11_06655"
     CDS             complement(3996..4352)
                     /locus_tag="PLQ11_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24616.1"
                     /translation="MRALATLLMFVVCAFQAEAYVADGHEYTLACNKDGFVLTSRASV
                     TRYIEAGVSSRFVKKKEKIFLGKNCDAIHEIFGGGKWCWANGGFGAEFSGTKIWFPRQ
                     ELICPRETDNHLGCNC"
     gene            complement(4358..5551)
                     /gene="sucC"
                     /locus_tag="PLQ11_06660"
     CDS             complement(4358..5551)
                     /gene="sucC"
                     /locus_tag="PLQ11_06660"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HRK24617.1"
                     /translation="MNIHEYQGKAVLKSFGCPVSAGFPAFTVAEAEEAAKKLPGPLFV
                     VKSQIHAGGRGKGKFKELPADAKGGVRLAKSVAEVKAFAKEMLGNTLVTLQTGPAGKQ
                     VNRLYIEDGSDIAKEFYLSMLVDRVTSRVSFVVSTEGGMNIEEVAHESPEKIITFSVD
                     PATGYMGHHGRAIAKALNLSGDLAKQAEALGAKLYAAFTAKDMSMLEINPLIVTKEGQ
                     LKVLDAKISFDSNALYRHADVMALRDETEEDAKEIEASKYDLSYIALDGTIGCMVNGA
                     GLAMATMDIIKLYGEEPANFLDVGGGASKEKVTAAFKIITADPKVKGILVNIFGGIMK
                     CDIIAEGVIAAVKEVGLKVPLVVRLEGTNVELGKSIISKSGLNVIPADDLDDAAQKIV
                     KAVKG"
     gene            complement(5669..6637)
                     /gene="mdh"
                     /locus_tag="PLQ11_06665"
     CDS             complement(5669..6637)
                     /gene="mdh"
                     /locus_tag="PLQ11_06665"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HRK24618.1"
                     /translation="MARRKIALIGAGQIGGTLAHLAALKELGDVVLFDIAEGTPQGKA
                     LDLAESAPVDGFDAGLKGANDYSAIAGADVVIVTAGVPRKPGMSRDDLIGINLKVMES
                     VGKGIKEFCPKAFVICITNPLDAMVWALQKFSGVKPNKIVGMAGVLDSARFRHFLADE
                     FKVSVKDVSAFVLGGHGDDMVPLTRYSTVAGIPLPDLVRMKWTTQERIDAIVDRTRKG
                     GGEIVALLKTGSAFYAPAASAIAMAESYLKDQKRVLPCAAALKNEYGADCNGLFIGVP
                     IVIGEKGVERIVKIKLKGEEQAMFAKSVASVKGLVAACKQINPSLA"
     gene            complement(6965..7561)
                     /locus_tag="PLQ11_06670"
     CDS             complement(6965..7561)
                     /locus_tag="PLQ11_06670"
                     /inference="COORDINATES: protein motif:HMM:NF037152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HRK24619.1"
                     /translation="MSTESVEARILAAAQTRLRREGLRRLTVVRIAEDAGMTHANVYR
                     FFKSKADLVDRLISDWLRDLERRLTDITQAPDPAHDKLERFLTLVSRNYADKARSDPR
                     LFDALIDALDQQRPAALRHRQRQRDLLLQVVEEGIATRLFAGSDPRRLRQTISDLMHR
                     FIDPHALRTAASIEPASFDARRERATRAVLRTLAAARI"
     gene            complement(7558..8733)
                     /gene="zapE"
                     /locus_tag="PLQ11_06675"
     CDS             complement(7558..8733)
                     /gene="zapE"
                     /locus_tag="PLQ11_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010683377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HRK24620.1"
                     /translation="MSGAVAERYRALVAEGRLEPDPAQQAAIERLDALAAQLEEGQLA
                     AKGSALGWLFSSKTRRDPIPGLYLWGSVGRGKTMLMDLFFDELKVRHKRREHFHAFMS
                     DVHERIYEWRRLAKAGRARGNDPVAPVARDLAREASVLCFDEFAVTDIADAMLLGRLF
                     ENLFAEGVTVVATSNREPDELYKDGLNRQLFVPFIRMIEQKMQVLRLDARTDYRMEKL
                     AGAEVFLTPANAATAEQLETIFRNLTAGAPARPTHLHVKGRTLTLGKTAHHVALVSFE
                     ELCDRPLGAADYLAIAQAFRMVIIRDVPAMDQSQRNAAKRFINLIDVFYDYGVKTVIS
                     AAVPAQDLYHGTSGPEVFEFDRTVSRLIEMRSSDYLARPHGRQAPDPDKAISGIVET"
     gene            8958..9566
                     /locus_tag="PLQ11_06680"
     CDS             8958..9566
                     /locus_tag="PLQ11_06680"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRK24621.1"
                     /translation="MGGTANEHTVVRRLSAFDHEDYAQHLLRLDEATRYLRFGMPAKD
                     QFLIDYANSCTLWDVVIYGYFVDGILRGAAELRPLGGDHNEGEAAFSVEAEFRGRGVG
                     SQLFEKIVRAARNRSYQKLYMSCLATNRAMQALARRFAAEMSFEPGGSLGVVEPGHRD
                     VNSLSGEAAEDAEAIFAIARLDLPGHSEIDPLGWFNSTARAV"
     gene            complement(9590..10222)
                     /locus_tag="PLQ11_06685"
     CDS             complement(9590..10222)
                     /locus_tag="PLQ11_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019904172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AprI/Inh family metalloprotease inhibitor"
                     /protein_id="HRK24622.1"
                     /translation="MRPVAFTLVCLAPLLAACESSMRLPPLDNPFGRSERVTPAPAPQ
                     PRLEPTPTERVDVAPLPSVTRQDLAPPPGAAATPPLNPIDPDPGIRNELPPIPPTPPV
                     TPPVVPQVADAAPARPTQSALTGTWQAREASGSACRVTLSSSPKLDLYNASSSGCQSR
                     DLQRVTAWELRGDDVYLYEPGGAVAARFKAQSRQMNGTLSKSGAPLSLSR"
     gene            10372..>10730
                     /locus_tag="PLQ11_06690"
     CDS             10372..>10730
                     /locus_tag="PLQ11_06690"
                     /inference="COORDINATES: protein motif:HMM:NF025903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HRK24623.1"
                     /translation="MPFVRIDLQDTVAPTRRRAIADAVHAALVESIGIPAEDRFQVVS
                     SHGDELIYDPGYLGIERTDGIVMVEVHLSVGRSLDLKRALYAAVAERLAALGIRKQDV
                     FVHLMETTLLNWSFGDGA"
BASE COUNT         1941 a         3299 c         3364 g         2126 t
ORIGIN      
        1 agcacccatt cgaggtaggg ctcgacaaac gaccaccccc ccatgttctt gggctcttcc
       61 tggcaccaga ccacatccgc cttcctgaag cgctgcatga ccttggtcag ggccttcagc
      121 gggaacggat aaagctgttc gacgcgcagc aggtagacat cgtccagatt gcgcttctca
      181 cgctcttcca gaaggtcaaa gtagaccttg ccggtgcaga tgatcacgcg gcggatcttg
      241 gcgtccttgg cgaggctgta tccggcgttg ccgccatccg cgtcatcgcg caggaagcgg
      301 tggaacgagg agtcagcgcc aagatcggcc aggctcgact tgcagcgcgg atgcctgagc
      361 agcgatttcg gcgtcatcag gatcagcggc ttgcggatgt cgcgcttcat ctgccggcgc
      421 aggatgtgga agtagttggc cggagtcgtg caattggcga cctgcatgtt gtcctcggcg
      481 cagagctgca ggaagcgctc gagacgggcg gacgaatgct ccgggccctg accctcaaat
      541 ccgtgcggca gcaggcagac cagacccgac aggcgcagcc atttgcgctc gcccgaggag
      601 atgaactggt cgaacacgac ctgagcgccg ttggcgaagt cgccgaactg gccctcccac
      661 agggtcagcg tgttgggttc aaccagcgaa tagccgtatt cgaatccaag caccgcctct
      721 tccgacagca tcgagttgat gacttcgtag cgcttctgtc cggcacgaag gttgttcagc
      781 ggggtatggc ggttctcatt gacctgatcg atgatcaccg aatggcgctg ggagaaggtg
      841 ccgcgctcgc aatcctggcc cgacagacga accgggtagc cctcgtcgac cagcgtgcca
      901 taggccagcg cctcggccat cgcccagtcc agcccctcgc cgctttcgac catcttgaga
      961 cggccgtcca tgaaacgctg gatggtgcgg tgggcgttga aaccctccgg gatggtggcg
     1021 atcttgcggc cgatggacct gagcgtgtcg atcgccacgc ccgtcttgcc atgcaacgga
     1081 tcgtcatggg catctgtgcc ggccttaagg ccttcccagg cgccttccag ccaatcggcc
     1141 ttgttgggct tgtaggcatg gccagcctcg aattccgcct ccaggcgcga gacccagtcg
     1201 gccttcatct tgtcgacttc gccttgcgtg acgactttct cggcaatcag cttttccgca
     1261 tagatctcca gcgtcgtccg gtggctgcgg atcttctgat acatcagcgg ctgggtgaag
     1321 gccggctcgt ccccttcatt gtggccgaag cggcgatagc accacatgtc gaccacgacc
     1381 ggcttctgga acttctggcg gaactcggcc gccaccttgc agacaaacac caccgcctcg
     1441 gggtcgtcgc cgttgacgtg gaagatcggc gcctcgacga tcttggccat gtcggacgga
     1501 tagggcgatg agcgcgagaa gcgcggatag gtggtaaagc cgatctggtt gttgatgatc
     1561 accgaaaccc agccgccggt gcgatggcct ttcaggcccg acatgccgaa gcattcggcc
     1621 accacgccct ggcccgcaaa agccgcatcg ccatgaatga gcagcggcag cacggcgcgg
     1681 cgatcctccg gcgtgcagtt gagctggtcc tgcttggcgc ggacgcggcc aagcacgacc
     1741 ggattgacga tttccagatg cgaagggttc ggtgtcagcg acagatgcac cttgttgcca
     1801 tcaaactcgc gatccgatga cgcgccaagg tgatatttca cgtcgccgga cccttccacc
     1861 tcatccggcg cgaaggagcc gcccttgaac tcgtggaaca gcgcacgatg cggcttggcc
     1921 atcacctggg tcagcacgtt caggcggcca cgatgggcca tgccgaaaac gatctccttg
     1981 acgccgagat tgccgccgcg cttgatgatc tgctcaagac cggggatcag cgcctcgccg
     2041 ccgtcaagac cgaaccgctt ggtgccggtg aatttcctgt cgaggaactt ctcgaagcct
     2101 tctgcctcga ccagcttgtt gagaatggcg cgctttccct cgcgggtgaa ggtgatctcc
     2161 ttttccggtc cctcgatgcg ggcctgcagc caggccttct gcgccggatc ggaaatgtgc
     2221 atgaactcat agccgatggt ggcgcaatag gtgcgcctca ggatcgccag catctcgcga
     2281 atggtggcaa attccagccc cagcacgtgg tcgatgaaga tcttgcggtc ccagtcctcc
     2341 tcggtgaacc cgtaggaaga cggatgcagt tcctcgtggt ccttgcgcgg ctcgatcccg
     2401 agcgggtcga gatcggcatg cagatggccg cgcatgcgat aggcgcggat catcatcagc
     2461 gcatgcaccg aatcgcgcgc cgctgcctgc aattgtgccg cagacggcgc cgcgccgccg
     2521 gctgccgcct tgcccttcag cttgtcggcc acggcctttt cgaccgacga ccagtcgctg
     2581 tcgagtgctg acgtcagttc ggtgcgggca acaaccggcc agttggagcg tttccaggac
     2641 ggtccgggcg tgacatcgcc ggtcagggcc tcaagatagg cggcattgct gcccgaaagc
     2701 gctgagagat ccagatcgcg atccattcga tcaccctttt ggtggttcaa tccacgatga
     2761 aaagtcttgc accagactgg gtgcgcgacc ggtgcggttc ggcgccgtcc gccacctgat
     2821 aactcatgcc cggcttgaga acgaaaacgc ggccatcggc cagcgttgtt tccaattcgc
     2881 cctcaagaca gagcaggata tggcctttct cgcaccagtg gtcggagaca taaccggccg
     2941 agtagtcgac aagacgcacg cggatggcgt ttccctccgg gccgaaggtc ctggtccgcc
     3001 aaatcgcctc gccttcggcg ccggggtggc gcgttggcgt gacagcggac cagtccgtcg
     3061 tcccgaacgg gatgtccgtc atccgcaagg gttacttgcc cttgagcacg tcgacgagcg
     3121 tcttgccaag acgcgccggc gagggcgaga cgcggatgcc cgctgcctcc atcgccgcga
     3181 tcttgtcctc ggcgccgccc ttgccgcccg agatgatcgc gcctgcatga cccatgcggc
     3241 ggccgggagg cgccgtgcgg ccggcgatga agccgaccat cggcttcttg cggccacgtt
     3301 ttgcctcgtc cctgatgaac tgcgccgcat cttcctcggc tgagccgccg atctcgccaa
     3361 tcatgacgat cgacttggtg gcctcgtcct tgaggaacat ctccagcatc tcgatgaatt
     3421 cggtgccctt gacggggtcg ccgccgatgc cgaccgcagt ggtctggccg aggccctcct
     3481 gcgtggtctg gaacaccgcc tcataggtca gcgtgccgga gcgcgagacg atgccgacat
     3541 tgcccttctt gaagatattg cccggcatga tgccgatctt gcattctccg gcggtcatca
     3601 cgccggggca gttcggcccg atcagccgcg atttggaccc cgacagggcg cgcttgacgc
     3661 ggatcatgtc ggtcaccgga atgccttcgg tgatgcagac gatcagcggg atttccgcct
     3721 gaattgcctc gcagatcgca tcggccgcgc cgggcggggg cacgtagatg accgaggcgt
     3781 ccgccccggt agcctcgcgg gcctcggcca cggtgtcgaa caccggcagg ccgagatgca
     3841 tcgagccgcc cttgcctggc gaggtgccgc cgaccatctg cgtgccgtag gcgatcgcct
     3901 gttcggagtg gaaagtgccg ttcttgccgg tgaagccctg gcagatgaca cgcgtgttct
     3961 tgtcgatgag gatagacatg tgttacttcc ggggttcagc agttacagcc gagatgattg
     4021 tcagtttctc ttggacagat gagttcttga cgcggaaacc aaattttggt gcctgaaaat
     4081 tcagctccaa aacctccatt cgcccagcac cactttccgc caccaaatat ttcatgaatt
     4141 gcatcgcaat tttttcccag aaagattttc tctttcttct ttacaaatcg cgatgatact
     4201 ccagcttcaa tgtaacgtgt gacggaagcc ctagaggtca gaacaaatcc atctttgtta
     4261 catgcaagcg tatattcatg gccatcggca acatatgctt ccgcttgaaa tgcgcagacc
     4321 acaaacatta aaagggtagc tagagcgcgc atccctctca gcccttcaca gccttgacaa
     4381 ttttctgcgc ggcatcgtcg agatcatcgg ccgggatcac attcagtccc gacttgctga
     4441 tgatcgactt gccgagctcg acgttggtgc cttcgaggcg caccaccagc ggaaccttga
     4501 ggccaacctc cttcaccgcg gcgatgacgc cctcggcgat gatgtcgcac ttcatgatgc
     4561 cgccgaagat gttgaccaga atgcccttca ccttcgggtc ggcggtgatg atcttgaacg
     4621 ccgccgtcac cttctccttg gatgcgccac cgccgacatc aaggaagttc gccggctcct
     4681 cgccgtagag cttgatgatg tccatcgtcg ccatggcgag gcccgcgccg ttgaccatgc
     4741 agccgatggt gccgtcgagc gcgatatagg acaggtcgta tttggaggcc tcgatctcct
     4801 tggcatcctc ctcggtctcg tcgcgcagcg ccatcacgtc ggcatgacgg tagagcgcat
     4861 tggaatcaaa gctgatcttg gcgtcgagca ccttgagctg accctctttg gtgacgatca
     4921 gcgggttgat ttccagcatg ctcatgtcct tggccgtaaa ggcggcatag agcttggcac
     4981 cgagcgcttc ggcctgcttg gcaagatcgc cggaaagatt cagtgccttg gcgatggcgc
     5041 ggccgtgatg gcccatgtag ccggtcgcag gatccaccga gaaggtaatg atcttctcag
     5101 gcgattcgtg cgccacttcc tcgatgttca tgccgccttc ggtcgagacc acgaaggaaa
     5161 cgcgcgaggt gacgcggtcc accagcatcg acaggtagaa ttccttggcg atgtcggagc
     5221 cgtcctcgat gtagaggcgg ttgacctgct tgccagccgg gccggtctgc agggtgacca
     5281 gcgtgttgcc gagcatttcc ttggcaaaag ccttgacctc ggcaaccgac ttggcgagac
     5341 gcacgccgcc cttggcatcg gcgggaagtt ccttgaactt gcccttgccg cggccgcccg
     5401 cgtggatctg gcttttcacg acgaacagcg gacccggcag cttttttgcc gcctcttccg
     5461 cctcggcaac ggtgaaggcc gggaagccgg ccgacaccgg gcagccgaag ctcttcagca
     5521 cggccttgcc ctggtattca tggatgttca tgtgcgtccc tctccggaag cgaaaattct
     5581 gaagcagcga tcggcagtcg gcagtcggca atcgaaaaat tctggcaatc cttacgattg
     5641 cctgttcccg attgccgatt gccccctctt acgccagcga cgggttgatc tgcttgcagg
     5701 cggcgaccag gcccttcacc gaagcgacgg acttggcgaa catcgcctgt tcctcgccct
     5761 tgagcttgat cttgacaatg cgctcgacac ccttctcgcc gatcacgatc ggcacgccga
     5821 tgaacaggcc gttgcagtcc gcgccatact cgttcttgag cgcggcggcg cagggcagca
     5881 cacgcttctg atccttgagg tagctctcgg ccatggcgat ggcggaggcc gcaggcgcat
     5941 agaaggccga gccggtcttg agcagcgcga cgatctcgcc gccgcccttg cgggtgcgat
     6001 ccacgatggc gtcgatgcgc tcctgcgtcg tccacttcat cctgaccaga tccggcagcg
     6061 gaatgccggc aacggtcgaa tagcgggtca gcggcaccat gtcgtcgcca tgcccgccca
     6121 gcacaaaggc cgagacgtcc ttgaccgaga ccttgaactc atcggcaagg aagtggcgga
     6181 agcgggccga atcgagcacg ccggccatgc cgacgatctt gttcggcttg acgccggaga
     6241 acttctgcag cgcccagacc atcgcgtcga gcgggttggt gatgcagatc acgaaggcct
     6301 tcgggcagaa ttccttgatg cccttgccga ccgattccat taccttgagg ttgatgccga
     6361 tcaggtcatc gcggctcatg cccggcttgc gcggcacacc ggcggtgacg atcaccacat
     6421 cggcgccggc aatcgccgag taatcattgg cgcccttcaa tccggcatcg aaaccgtcga
     6481 cgggagcgga ttcggccagg tcgagcgcct tgccctgtgg cgtgccttcg gcaatatcga
     6541 acagcacgac atccccgagt tccttgaggg cagcaaggtg agcaagcgtg ccgccgatct
     6601 gtccggcacc gatgagagcg atcttccgac gcgccatggc ctgatatcct aatatctggg
     6661 ttgaggtggc tggaacctag ccagtttgcg ctgtttcgcc cacccggcct ttgggatgag
     6721 gcgccccctc atcccgtatg ggcggcctct gctggtgcgg cttgtaggtg agagcgccat
     6781 tccggatcaa gcttgcggct ggctggatgc ctgcatgctt tggtcttaga ccacgttcgt
     6841 tggccagcgg caggagttcg tgttatttaa aatcaatagc ttagctagtc accaaaagca
     6901 gccagcgtgc gttctggccc agaaaaaagc cataacaaat tacaatattt gtaatttgtt
     6961 atggctagat gcgggcggcg gccaatgttc tgagcacggc ccgggtcgcc cgttcgcggc
     7021 gcgcgtcaaa cgaggccggc tcgatgctcg cggcggtgcg cagtgcatgc gggtcgatga
     7081 agcgatgcat caggtcggag atggtctgtc gcagccggcg gggatcgctg ccggcaaaca
     7141 ggcgggttgc aatgccctcc tcgacgacct ggagcagaag atcgcgctgg cgctgccggt
     7201 ggcgcagggc ggcggggcgc tgctggtcga gggcgtcaat cagcgcgtcg aacaatcggg
     7261 ggtcggagcg ggccttgtcg gcataattgc gtgaaaccag ggtcagaaag cgttccagct
     7321 tgtcatgcgc cggatcgggc gcctgggtga tgtcggtgag gcgacgttcg agatcgcgca
     7381 gccagtcact gatcaggcga tcgaccagat ccgccttcga cttgaagaac cggtagacat
     7441 tggcgtgggt catgccggcg tcctcggcaa tccgcacaac cgtcagtcgg cgcaggccct
     7501 cccgccgcaa ccgcgtctgg gcggcggcga ggatccgcgc ttcgacgctt tccgtgctca
     7561 tgtctcgacg atgccgctga tcgccttgtc cgggtcgggt gcctggcgac cgtgcgggcg
     7621 ggcgagatag tcgctggagc gcatctcgat caggcgggag acggtgcgat caaattcgaa
     7681 cacttccggg ccgctggtgc cgtgatagag atcctgcgcc ggaaccgctg cagaaatcac
     7741 cgtcttgacg ccgtaatcgt agaatacatc gatgagattg atgaagcgct tggcggcgtt
     7801 gcgctggctc tggtccatgg ctggcacgtc gcgaatgatc accatccgaa aagcctgggc
     7861 gatcgccagg taatcggctg cgcccagcgg cctgtcacac agttcctcga acgagaccag
     7921 ggcgacatga tgggcggtct tgccgagagt cagcgtgcgc cccttgacgt gcagatgtgt
     7981 cggtcgggcc ggggctccgg ctgtcaggtt ccggaaaatc gtttcgagct gctctgctgt
     8041 ggccgcgttg gccggcgtca gaaagacctc ggctccggcc agcttttcca tccggtagtc
     8101 ggtgcgggca tcaagccgca ggacctgcat cttctgctcg atcatccgga tgaacggcac
     8161 gaacagctgc cggttgagac cgtccttgta gagctcgtca ggctcgcggt tcgaggtggc
     8221 gacgacagtg acgccttcgg caaacaggtt ctcgaacaac cggccaagca gcatggcgtc
     8281 ggcgatgtct gtcaccgcaa attcgtcaaa gcacaggacg ctggcctcgc gggcaagatc
     8341 gcgggccacc ggtgccaccg ggtcgttgcc ccgcgcccgc ccggccttgg ccagccgtcg
     8401 ccattcatag atgcgttcgt gaacatcgct catgaaggcg tggaaatgct cgcgccgctt
     8461 gtgccgcacc ttcaactcgt caaaaaacag atccatcagc atggtcttgc cacgtccgac
     8521 cgatccccag agatacaatc cggggatcgg atcgcgcctg gtcttgctgc tgaacagcca
     8581 gccaagagcg ctgcccttgg cagccagctg cccctcttcg agctgagctg ccagggcatc
     8641 gagccgctcg atggcggcct gctgggcagg gtcgggctct agccgccctt cggccaccag
     8701 cgcccgatag cgttctgcca ctgccccgct catcctcact ccccggatgt ttgtcaccat
     8761 ctcgtcacga tcaccgacta ttcaatccgc atctctttca caagccgcct tggggcgagg
     8821 cgtgagagga agatatgcat tcttgaaatg ttagtctttc tgacatatct aataatgcat
     8881 gtctggccga atcggccgag gaaacgagac gtaaggaagg ctcaattgtt ggacaccgtt
     8941 gccttaagga ggctgacatg ggtggcacag cgaatgagca cacggttgtg cggcgtctgt
     9001 ccgcattcga ccatgaagat tatgcccagc atctgctgag gctcgatgaa gcgacgcgct
     9061 atctgcgctt cggcatgccg gccaaggatc agttcctgat cgattatgcc aacagctgca
     9121 ccctctggga tgtggtgatc tacggctatt tcgtcgacgg cattctgcgt ggagcggccg
     9181 aattgcgtcc actgggcggc gatcataacg aaggcgaggc ggcgttttcg gtggaggccg
     9241 aatttcgcgg ccggggtgtc ggctcgcagc tgttcgaaaa gatcgtccgc gcggcgcgca
     9301 atcgcagcta ccagaagctc tatatgagct gccttgccac caatcgcgcc atgcaggcgc
     9361 tggcccggcg ttttgctgcg gaaatgagtt tcgagccggg tggttcgctc ggtgtggtcg
     9421 agcccggcca tcgcgatgtg aattcgctga gcggcgaggc cgccgaagat gccgaagcaa
     9481 tcttcgccat cgccaggctt gatctgccgg gacattcgga gatcgatccg ctcggctggt
     9541 tcaactcgac cgcgcgcgcg gtctgacgag cgtcctgccg cagcgccgtt cagcgactga
     9601 ggctgagcgg cgccccggat ttcgacagcg tgccgttcat ctggcggctc tgcgccttga
     9661 aacgggccgc gaccgcgcct cccggctcgt agagatagac gtcatcgccg cgcaattccc
     9721 aggcggttac gcgttgcagg tcacggctct ggcagccgct gctgctggca ttgtagagat
     9781 cgagcttcgg gctagaggac aaggtgacgc ggcaggcact gccggaggct tcccttgcct
     9841 gccaggtccc ggtcaaggcg ctttgcgtcg gtcgggccgg cgcggcatcc gccacctgcg
     9901 gcacaacggg gggcgtcacg ggtggtgtcg ggggaatcgg cggtaattcg ttccgaatgc
     9961 caggatccgg gtcgatcgga ttgagcggcg gcgtcgctgc ggcgcccggc ggaggggcca
    10021 gatcctgcct ggtcaccgat ggcaaggggg cgacatcaac gcgctcggtc ggcgtcggct
    10081 caaggcgcgg ttgcggcgct ggcgccggcg tcacgcgctc ggagcggccg aacggattgt
    10141 cgagcggggg caaccgcatg ctggattcgc acgccgcaag cagcggggca agacagacga
    10201 gcgtgaaagc tacagggcgc acggggacat ccttcttcgg cttgtgacct gaggcatagc
    10261 ggaggattgc ggcaaaacaa gccccgggcg gcggcgggca ggaaattccg gggagggttt
    10321 gactttgcca ataaagaaac atactagtag gtttgtttat tggagatgcc catgcccttc
    10381 gtccgaatcg atctgcaaga cactgttgcg ccaacgcgtc gccgcgccat cgccgatgcg
    10441 gtgcatgccg ctcttgtcga aagcatcggc attccggccg aggaccgctt tcaggtggtt
    10501 tcatcgcatg gcgatgagct gatttacgat cccggctatc tcgggatcga gcgcacggac
    10561 gggatcgtca tggtggaggt gcatctgtcg gtcgggcgca gcctcgatct caagcgggcg
    10621 ctttatgcgg cggtggcgga gcggctggcc gcgctgggaa ttcgcaagca ggatgtgttc
    10681 gtgcatctga tggagacaac cctgctcaac tggtcgttcg gggacggggc
//
LOCUS       DAOMXG010000076        10717 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_3283_length_10717_cov_9.838210, whole genome shotgun sequence.
ACCESSION   DAOMXG010000076 DAOMXG010000000
VERSION     DAOMXG010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 10717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10717
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3283_length_10717_cov_9.838210"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="PLQ11_06695"
     CDS             <1..321
                     /locus_tag="PLQ11_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HRK24624.1"
                     /translation="QLRLAEGIGHVAQDAAAIAAVRSGFARLADPVALAAAQVLVTLA
                     GSALIPLMLIDGAMEAEAAFDAASVDEDWNTQLWGEDAEALERRAYRRNEFLAAAGLV
                     AALS"
     gene            complement(334..705)
                     /locus_tag="PLQ11_06700"
     CDS             complement(334..705)
                     /locus_tag="PLQ11_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HRK24625.1"
                     /translation="MLRSALAIAAVVALPSLAMAQADPILGSWTRDTGASKINFAPCG
                     GAICGTLTWVKDTSGPAKVGQRVFTNVKQTSPGKYSGTAFNPEDGKSYSGTLTVNGNS
                     LSGGGCVLGGLICRTVNYRRG"
     gene            915..2447
                     /locus_tag="PLQ11_06705"
     CDS             915..2447
                     /locus_tag="PLQ11_06705"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HRK24626.1"
                     /translation="MKDILEKLEQKRASARIGGGQGRIDAQHSKGKLTARERIELLLD
                     KESFEEYDMFVEHRSNEFGMEKSRISGDGVVTGWGTINGRTVFVFAKDFTVFGGSLSE
                     THAQKICKVMDMAVKMRAPVIGLYDAGGARIQEGVAALGGYGEVFRRNVAASGVIPQI
                     SVIMGPCAGGDVYSPAMTDFIFMVKDTSYMFVTGPEVVKTVTNETVTAEELGGASVHT
                     VKSSVADGAYENDIEALLQVRRLIDFLPANNIDGAPEWPSFDDKNRYDHSLDTLVPDN
                     PNKPYDVKELIVKVVDESDFFEIQENFAKNIVVGFGRLEGSTVGFVANQPLVLAGVLD
                     SDASRKAARFVRFCNAFKIPIVTFVDVPGFLPGTAQEYGGLIKHGAKLLFAYSQCTVP
                     LVTVIIRKAYGGAYDVMASKHIGADINYAWPSAQIAVMGAKGAVEIIFRSDMNDPDKI
                     AERTKEYEQRFLSPFVAAERGYIDEVIQPFGTRKRVARALAMLRTKKLEQPWKKHDNM
                     PL"
     gene            2454..2693
                     /locus_tag="PLQ11_06710"
     CDS             2454..2693
                     /locus_tag="PLQ11_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HRK24627.1"
                     /translation="MPTVLRFDGCRAFFYSNEGHEPPHLHVIRDNKEAKFWLHDLSVA
                     VNLGFAAHEINAILIVLRENRVLLAEAWNDHFGNR"
     gene            2671..2925
                     /locus_tag="PLQ11_06715"
     CDS             2671..2925
                     /locus_tag="PLQ11_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HRK24628.1"
                     /translation="MTILEIDDADIRAVAVEFSDSELIVRLADGRRIATPLDWYPRLA
                     AAALADRQKIEISPLGLHWPALDEDLSIAAMLAGRKARAA"
     gene            3116..5281
                     /locus_tag="PLQ11_06720"
     CDS             3116..5281
                     /locus_tag="PLQ11_06720"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HRK24629.1"
                     /translation="MAYVSTHGIQVAKILHDFLVKEALLGTGVSAEQFLAGMADVVKA
                     ITPKHRRLLKHRDKLQAQIDAWHRARKGKAIKQAEYEAFLKEIGYLLPEPAEGRITTK
                     NVDAEIAKIAGPQLVVPCSNARYALNAANARWGSLYDALYGTDAIADEGDLKRGRGYN
                     AARGAAVVARARAILDGAAPLARGSHADATGYSVENGALKVALKKGVTGLRNKKAFVG
                     YRGDAARPTAILLAHNGLHFEIVIDAKHAIGKADPAGVADVMVESALTTIMDMEDSVA
                     AVDAEDKVGVYRNWLGLMKGDLSEKMEKDGKSFTRKLNPDRVYTPARGGEVPWPLHGR
                     SLMLVRNVGMHMMTDAVLDAKGRELPETIIDCCISSMIAMHDLKKTSGLRNSRTGSVY
                     IVKPKMHGPDEVALASKLFGRVEKMLGLPANTLKMGIMDEERRTTVNLKACILAAKAR
                     VVFINTGFLDRTGDEIHTSMEAGPMVRKAEMRAASWLPAYEDNNVDVGMATGLPGKAQ
                     IGKGMWAMPDRMADMMAQKIGHPKSGANTAWVPSPTAATLHALHYHEVDVFARQAEIA
                     GRKRADLSAILTVPVAKSNFEPAAVQQELDNNAQGILGYVVRWIDAGVGCSKVPDIHD
                     VGLMEDRATLRISSQHIANWLHHGVCTRAQVRSTLKRMAKVVDRQNAGDPTYRPMAPA
                     YDGVAFKAACDLVFKGLKQPNGYTEFVLTRRRREAKAAG"
     gene            5362..6423
                     /locus_tag="PLQ11_06725"
     CDS             5362..6423
                     /locus_tag="PLQ11_06725"
                     /inference="COORDINATES: protein motif:HMM:NF013890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HRK24630.1"
                     /translation="MAGRVVRAVRCLLPLVLVAGCVQLHPDQKGGEAIVRIGDFDSRD
                     KGPIRVGDIAGYYVPYALLAVWAYGSGENDRGERWAKLMAMNDRQGQPLKPRINSWMQ
                     QWTWIHKGFAEDVLPCSNKRIEEGRCHTPLGGIGVHTFVRKDCGEVVVAFRGTDFTEV
                     NDWITNFHWFTRLLPIYDQYEQVQDFAPEIVEIMRQQCPRQKSLRLTAVGHSLGGGLA
                     QQMAYASAEIQRVYAYDPSMVTGYYDVDSAARQKLNHLEIDRAYEHGEVLAYFRYLMR
                     QFYPPSACNPQIRNVRFDFLNGSIVDQHSIGDLAGHLVEAAGSKDQWERRRGKYRLPV
                     GSDADPGMCRGTGAGAAPR"
     gene            complement(6427..7551)
                     /locus_tag="PLQ11_06730"
     CDS             complement(6427..7551)
                     /locus_tag="PLQ11_06730"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HRK24631.1"
                     /translation="MAKNNRSHARARACAFLEKVPLLDAHNDLPYVVRKSAAAGDVAA
                     YDLTREHQETDTDIPRLVAGRLAGQFWAAFVPTDIENPVRFTLEQIALIKRMNSMHAD
                     VFHPAFKAADLARARRLGKIASYIAVESGVGLRNSLELLPLWHELGVRYLTLCHNETL
                     DWIDSATDAPRHGGMTDLGRKVIAECNRLGILVDLSHTSHQAQRQALDCARAPLAITH
                     ANAFALVDHPRNVKDDVLAQLKSNGGIVCATFVPTFSNQATRNWLKGVCDPYGKALHG
                     DLDTLWARHAETAGPCPRATLTQVADHLEYMTGIAGIDHIGIGSDFYGGHVPEGLESV
                     ARFPDFIAELMLRGWSEAYLAKICSRNLLRVLRGVEKAAG"
     gene            7797..8213
                     /locus_tag="PLQ11_06735"
     CDS             7797..8213
                     /locus_tag="PLQ11_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24632.1"
                     /translation="MSAQALAAPAARLQLVPKGEAVRAADEPGIDVSFKLPMTRREAE
                     ILFEIDGAQTGQSSAWKTFFVETLLEHLVFASRPTGRISNEDASWLLSLVGEDWSPSV
                     PALMRALVLQAEDVPQSLIAFAMRCGAMRCGASSLV"
     gene            8286..10292
                     /locus_tag="PLQ11_06740"
     CDS             8286..10292
                     /locus_tag="PLQ11_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003599287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HRK24633.1"
                     /translation="MFGKILIANRGEIACRVIKTARKMGIKTVAVYSEADKDALHVSM
                     ADEAVFIGPPPAAQSYLVIDKIIAACKSTGAEAVHPGYGFLSEREAFAEALKAAKITF
                     IGPNPKAIAAMGDKIESKKFANAAKVSTVPGYLGVIEGPEQAVRIADEIGYPVMIKAS
                     AGGGGKGMRIAYSRDEVADGFVRAKNEAAASFGDDRVFVEKFIVDPRHIEIQLIGDKH
                     GNVVYLNERECSIQRRNQKVIEEAPSPLLDAATRKKMGEQAVALAKAVNYDSAGTVEF
                     VAGQDKSFFFLEMNTRLQVEHPVTELITGIDLVEEMIRVAAGEKLRFKQSDVGINGWA
                     VESRVYAEDPVRNFLPSTGRLIKYRPPTEGTFGDVTVRNDTGVYEGGEISVYYDPMIA
                     KLVTHAPTRLGAIKAQAEALDAFAIEGIRHNIPFVSALMHHPRWQKGALSTGFIKEEF
                     PDGFVAPAPEGEVALRMAAVAAWLDHTHNVRRRLIDHQMATGKKFEFSRERSVMLGKQ
                     RINLLVHGAADGLVIEFADTGRKVEVVAETQIGDPLWQGTIGNDRVSAQVRPVLNGYW
                     LAHHGTEAVALVFTRREADLWALMPEKVAADTSKKLLCPMPGLVKQVAVIEGQEVKAG
                     DILAVVEAMKMENVLRAERDVTVKAIKARAGDSLAVDAVIMEFA"
     gene            complement(10296..>10717)
                     /locus_tag="PLQ11_06745"
     CDS             complement(10296..>10717)
                     /locus_tag="PLQ11_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24634.1"
                     /translation="RKGAPDTFDAAGYTAMLARIRAAAGTDETVYAPAFRREIEEPIA
                     GAIAVRPEHRLVITEGNYLLCDGAWSGVRPLLDACWYVGIRVEDRIQRLIARHMSFGR
                     SRQAATEWVLRSDEANARLIETMRARADRQVTWPPPD"
BASE COUNT         2025 a         3202 c         3573 g         1917 t
ORIGIN      
        1 cagctgcggc tggccgaagg catcggccat gtcgcgcagg atgcggcggc gatcgcagcg
       61 gtgcggagcg gctttgcacg gcttgccgat ccggtggcgc tggcggcggc ccaggtgctg
      121 gtaacgttgg caggttcggc gctgatcccg ctgatgctga tcgacggtgc gatggaggcg
      181 gaagcagcgt tcgacgctgc ttcggtcgat gaggattgga acacccagct ctggggcgag
      241 gatgcggagg cgcttgaacg acgcgcctac cgccgcaacg agttcctcgc ggcggcgggg
      301 ctggtcgcag ctctatcgta aggaacccgc gggtcagccg cggcggtaat tcaccgtgcg
      361 gcagatgagg ccacccagaa cgcagccgcc gcccgacagg gaattgccgt tgacggtcag
      421 cgtgccgctg tagctcttgc catcctcggg gttgaaggcg gtgccggaat atttgccggg
      481 cgaggtctgc ttgacgttgg tgaacacgcg ctggccgacc ttggccgggc cggaggtgtc
      541 cttcacccat gtcaatgtgc cgcagatcgc gccgccgcag ggggcgaaat tgatcttgga
      601 agcgccggta tcgcgcgtcc agctaccaag gatcggatcg gcctgagcca tcgccagtga
      661 aggcaaggcg acgacagcgg caatcgcaag agcagagcga agcatggttt cctcccgttt
      721 atggcctcat gggccttggt ctgactatag gcggctgcca gccggccgca agatcatata
      781 tgaaccataa ccgttttatg gctgaagcgc cgatgaacgg cacagccggg ctgtgctgcg
      841 tccccaaagg tctgattgac ccggtgcagc cacctgttat ccgtggtcgg attgcgactg
      901 agcggagacc cccgatgaaa gacatcctcg aaaagcttga gcagaagcgc gccagcgccc
      961 gcatcggggg tggacagggg cgcatcgacg cccagcacag caagggcaag ctgaccgccc
     1021 gcgagcgcat cgagctgttg ctcgacaagg aatccttcga ggaatacgac atgttcgtcg
     1081 agcatcgctc gaacgaattc ggcatggaaa agtcccgcat ttccggcgac ggcgtcgtca
     1141 ccggctgggg cacgatcaac gggcgcacgg tgttcgtgtt cgccaaggac ttcaccgtgt
     1201 tcggcggctc cctgtcggag acccatgcac agaagatctg caaggtcatg gacatggccg
     1261 tgaagatgcg cgcgccggtg atcggacttt acgatgcggg tggcgcgcgc attcaggagg
     1321 gcgtggcggc gctcggcggc tatggcgagg tgttccgccg caatgtggcg gcctcgggcg
     1381 tcattccgca aatctcggtg atcatggggc cgtgcgcggg cggcgacgtc tattcgccgg
     1441 cgatgaccga cttcatcttc atggtcaagg atacgtccta catgttcgtc accggaccgg
     1501 aggtggtgaa aaccgtcacc aacgagaccg tgacggccga agaactcggc ggcgcctcgg
     1561 ttcatacggt caaatcctcg gtggccgatg gcgcctacga gaacgacatc gaggcgctcc
     1621 tgcaggtgcg ccggctgatc gatttcctcc ccgccaacaa cattgacggg gcgccggaat
     1681 ggccgtcgtt tgacgacaag aaccgctacg atcacagcct cgatacgctg gtgccggaca
     1741 atccgaacaa gccgtatgac gtcaaggagc tgatcgtcaa agtggtcgac gagagcgact
     1801 tcttcgagat tcaggagaat ttcgccaaga acatcgtcgt cggcttcggg cgtctggaag
     1861 gttcgacggt cggcttcgtc gccaatcagc cgctggtgct cgccggcgtg ctcgacagcg
     1921 acgccagccg caaggcggcg cgctttgtcc gcttctgcaa cgccttcaag atcccgatcg
     1981 tcacttttgt cgatgtgccg ggcttcctgc cgggaacggc gcaggaatac ggcgggctga
     2041 tcaagcatgg cgccaagctg ctgttcgcct attcgcaatg cacggtgccg ctggtcacgg
     2101 tgatcatccg caaggcctat ggcggcgcct atgacgtgat ggcctccaag cacatcggcg
     2161 ccgatatcaa ctacgcctgg ccgagtgcgc agatcgccgt gatgggcgcc aagggcgcgg
     2221 tggagatcat cttccggtct gacatgaacg accccgacaa gatcgccgag cgcacgaagg
     2281 aatacgagca gcgcttcctg tcgccgttcg tcgccgccga gcgcggttat atcgacgagg
     2341 tgatccagcc gttcggcacg cgcaagcgcg tcgcccgcgc gctcgccatg ctgcgcacca
     2401 agaagctcga acagccctgg aagaagcacg acaacatgcc gctgtgaggg caggtgccca
     2461 ccgttctgag atttgatggc tgtcgtgcct tcttttacag caacgagggg cacgagccgc
     2521 cgcaccttca tgtcatcaga gacaacaagg aagccaagtt ctggcttcac gacctctcag
     2581 tcgcggtgaa cctcggtttt gcggcgcatg aaatcaatgc tatcctgatc gtgctgcgag
     2641 agaacagggt gctgctcgcg gaggcgtgga atgaccattt tggaaatcga tgacgcggat
     2701 atcagagctg tcgcggttga attcagcgac agcgaactga tcgtccgctt ggccgatggg
     2761 cgtcggatcg cgacgccgct cgactggtat ccacgactgg ccgccgccgc actggcggac
     2821 cgtcagaaga tcgagatttc ccctttgggc ttgcactggc cagcgctgga tgaggatctt
     2881 tcgatcgcgg ccatgcttgc ggggcgaaag gcgcgcgccg cctgaaaccg cggtttcgac
     2941 ctgcgcacca agaagctcga acagccctgg aagaagcacg acaacatgcc tttgtgaggc
     3001 ggaagcgccc atttccccat aaaaattgaa aatcaaacgg aaacgcaggc tttcgcgtcc
     3061 gcgcgcgctg tcgtaagacg cccccaacct gtcagaaccc ccgcaaggag cccccatggc
     3121 ctacgtcagc acccacggca ttcaggtcgc aaaaatcctg cacgacttcc ttgtcaagga
     3181 ggccctgctg ggaacggggg tttcggccga gcagttcctt gccggcatgg ccgatgtggt
     3241 caaggcgatt acgcccaagc atcgccggct gctcaagcat cgcgacaagc ttcaggcaca
     3301 gattgacgcc tggcaccgcg cgcgcaaggg caaggcgatc aagcaggccg aatacgaggc
     3361 gttcctgaag gagatcggct atctgctgcc cgagccggcc gaaggcagga tcaccacgaa
     3421 gaacgttgat gccgagattg cgaagatcgc cggcccgcag ctggtggtgc cgtgctccaa
     3481 cgcccgctat gcgctcaacg ccgccaatgc ccgctggggc agcctctatg atgcgctcta
     3541 cggcaccgat gcgattgccg atgaaggcga cctcaagcgc ggcaggggct acaatgcggc
     3601 ccgcggcgcg gcggtggtcg ccagggccag agccattctc gatggcgcgg caccgctcgc
     3661 ccgcggcagc catgcggatg cgaccggcta ttcggtcgag aatggtgcgc tcaaggttgc
     3721 attgaaaaag ggcgtaaccg gcctgaggaa caagaaggcc ttcgtcggct acaggggcga
     3781 tgccgcccgt ccgacggcga tcctgctggc acataacggc ctgcattttg agatcgtcat
     3841 cgatgccaaa catgccattg gcaaggccga tccggccggc gttgccgatg tcatggtcga
     3901 atcggcgctc accaccatca tggacatgga ggactcggtc gcagccgtcg atgccgagga
     3961 caaggtcggg gtctaccgca actggctcgg cctgatgaag ggggacctgt ccgagaagat
     4021 ggagaaggac ggcaagtcct tcacccgcaa gctcaatccg gatcgggtct ataccccggc
     4081 gcggggcggc gaagtgccgt ggccgctgca tggccgctcg ctgatgctgg tgcgcaacgt
     4141 cggcatgcac atgatgaccg acgcggtgct ggatgcgaag ggccgggaac tgccggagac
     4201 gatcatcgat tgctgcattt cgtcgatgat cgccatgcat gacctcaaga aaacctccgg
     4261 tttgcgcaat tcgcgcaccg gctcggtcta tatcgtcaag ccgaagatgc acggcccgga
     4321 cgaggtggcg ctggcgagca agctgttcgg ccgggtcgag aagatgctcg gcctgccggc
     4381 caatacgctg aaaatgggca tcatggatga ggagcgccgc acgacggtca acctcaaggc
     4441 gtgcatcctg gccgccaagg cgcgcgtcgt gttcatcaac accggattcc tcgatcgcac
     4501 gggcgatgaa atccacacct cgatggaagc cggcccgatg gtgcgcaagg cagaaatgcg
     4561 cgccgcctcg tggctgccgg cctatgagga caacaacgtc gatgtcggca tggcgaccgg
     4621 tctgccgggc aaagcgcaga tcggcaaggg catgtgggcc atgcctgatc gcatggctga
     4681 catgatggcc cagaagatcg gccatccgaa atccggcgcc aacaccgcct gggtgccgtc
     4741 gccgaccgcc gcgacgctgc atgcactgca ctatcacgag gtcgatgtgt tcgcccggca
     4801 ggcggagatc gccggccgca agcgcgccga tctgtccgcc atcctcaccg tgccggtggc
     4861 gaagtcgaac ttcgagccgg cagcggtgca gcaggaactc gacaacaatg cgcagggcat
     4921 tctcggctat gtcgtgcgct ggatcgacgc gggcgtgggc tgctcgaagg tgccggacat
     4981 tcacgatgtc gggctgatgg aggatcgcgc gacgctgcgc atctcctccc agcatatcgc
     5041 caactggctg catcacggtg tctgcaccag ggcgcaggtc cgctcgacgc tcaagcgcat
     5101 ggccaaggtc gtcgaccggc agaacgccgg cgatccgacc tacagaccga tggcgccggc
     5161 ctatgacggc gttgccttca aggcggcctg cgatctcgtt ttcaaggggc tcaagcagcc
     5221 gaacggctac accgaattcg tgctgacaag gcgtcgtcgc gaggccaagg ctgcggggtg
     5281 acatcacccg aacggaggtt gcatgtttcg tccccgggat cacaagtcga tcccgggttt
     5341 tttatctgcg aggcggcggg gatggcgggt cgggtagtgc gcgcggtgcg gtgcctgttg
     5401 ccgctggttc tggtggcagg ttgcgtccag ttgcaccctg accagaaggg cggcgaggcc
     5461 atcgtcagga tcggcgattt cgacagcaga gacaaaggcc cgatccgggt cggcgatatt
     5521 gccggctatt acgttcccta tgcgctgctg gcggtgtggg cttacggctc cggcgaaaac
     5581 gaccgcggcg agcgctgggc caaattgatg gcgatgaacg atcgccaggg gcaaccgctc
     5641 aagccgcgta tcaacagctg gatgcagcaa tggacctgga tccacaaggg atttgccgaa
     5701 gacgttctgc catgcagcaa caagcgcatc gaggaagggc gctgccatac accgcttggc
     5761 ggcatcggcg tgcatacttt tgtccgcaag gattgcggtg aggttgtcgt tgccttccgg
     5821 ggcacggatt tcaccgaggt gaatgactgg atcaccaatt tccattggtt tacccgcctt
     5881 ctgccgattt atgaccaata cgagcaggtg caggatttcg cgccggaaat cgtcgagatc
     5941 atgcggcagc aatgcccgag gcagaagtcg ttgcggctga ccgccgtcgg gcattcgctc
     6001 gggggcggac tggcccagca gatggcctat gcgagcgccg agatccagcg cgtctatgcc
     6061 tatgatccgt ccatggtcac cggctattac gatgtcgatt cagcggcgcg gcaaaagctg
     6121 aatcaccttg agatcgaccg cgcctatgaa catggcgaag tgctggccta tttccgctac
     6181 ctcatgcgcc agttctatcc accaagcgcc tgcaacccgc agatcaggaa tgtccggttc
     6241 gactttctca atgggagtat cgtcgatcag cattccatcg gagatttggc cggccatctg
     6301 gtcgaggcgg ccggcagcaa ggaccagtgg gagcgccgcc ggggcaaata ccggctgccg
     6361 gtcgggtcgg atgccgatcc cgggatgtgc cgaggcaccg gggcgggcgc cgcgccgcgg
     6421 tgaggctcag cctgccgcct tctccacacc cctgagcacg cgcagcaggt tgcgggagca
     6481 gatttttgcg agataggcct cgctccagcc gcgcagcatc agttcggcga tgaaatcggg
     6541 gaagcgggcg acgctttcca gcccttcggg gacatggccg ccatagaagt ccgagccgat
     6601 gccgatatgg tcgatgccgg cgatgccggt catgtattcg agatggtcgg caacctgggt
     6661 caaggtagcg cgcgggcagg ggccggcggt ttcggcatgg cgggcccaga gcgtgtcgag
     6721 atcgccgtgc agggccttgc catagggatc gcagacccct ttcagccaat tgcgtgtcgc
     6781 ctgattggaa aaggtcggca cgaaggtcgc gcagacgatg ccgccattgg atttgagctg
     6841 cgccagcacg tcatccttca cgttgcgtgg atgatcgacc agggcaaagg cattggcgtg
     6901 ggtgatggcc agcggcgcgc gggcgcagtc gagggcctga cgctgcgcct ggtgcgaggt
     6961 atgcgacaga tcgacgagaa tgccgagccg gttgcattcg gcgattacct tgcggccgag
     7021 atcggtcatc ccgccatggc gcggcgcgtc ggtcgcgctg tcgatccagt cgagggtctc
     7081 gttgtggcaa agcgtcagat aacgcacgcc gagttcgtgc cagagcggca gcagctccag
     7141 cgaattgcgg agcccgacgc cgctttcgac cgcgatgtat gaggcgattt tgccaagacg
     7201 tcgggcgcgg gccagatcgg ctgccttgaa ggccgggtga aacacatcgg catgcatgct
     7261 gttcatgcgc ttgatcagcg caatctgttc gagcgtgaag cggacaggat tttcgatgtc
     7321 ggtgggcacg aacgccgccc agaactggcc cgccagcctg ccggcgacga ggcgcgggat
     7381 atcggtgtcg gtctcctgat gctcgcgcgt caggtcatag gcggccacat cgccggccgc
     7441 agccgatttg cgcaccacat agggcaggtc gttgtgcgcg tcgagcagcg gcaccttttc
     7501 cagaaaggcg caggcgcggg cgcgggcgtg gctgcggttg ttcttggcca tggcggttca
     7561 tcaatcgttt gcgcaagtcg gaatccgagc aatgcgggtt tttcctcgca acgcaagggc
     7621 tgacgatcgc cgcaggcccc cggctgagcg cattcatgca attgcaacca agccttgttg
     7681 ccggctgtta aggttgcggc ggattcacct tgcctgttta ccggctgttt gagcggtagc
     7741 ggcattgtcg accccggaga gaagcacaag cttccgacag ggactggggt cagacaatga
     7801 gtgcacaggc tttggcggct ccggccgcgc ggttacaact ggtgccgaaa ggcgaggctg
     7861 tccgcgcggc cgatgagccg ggaatcgatg tcagtttcaa gctgccgatg acccggcgcg
     7921 aggcggagat cctgttcgag atcgacggcg cacagaccgg ccagagcagc gcctggaaaa
     7981 cgtttttcgt cgagacgctg ctcgaacatc tggtctttgc cagccggccg accgggcgga
     8041 tctccaacga ggatgcgtcc tggctgctca gtctggtcgg cgaggactgg agcccttcgg
     8101 ttccggcgct gatgcgggcg ctggtgttgc aggccgagga cgtgccgcaa tcgctgattg
     8161 cctttgccat gcgttgcggc gccatgcgct gcggtgcttc gtcattggtc tgagagcaag
     8221 cccccttacg cccttcgcgt tttccgatac aaagccttcg acatcgacag atcgagggcg
     8281 tttccatgtt tggcaaaatc ctgattgcga accggggcga gattgcctgc cgcgtcatca
     8341 agacggcgcg gaagatgggg atcaagaccg tcgcagtcta ttcggaggcc gacaaggacg
     8401 ccctgcatgt gtcgatggcg gatgaggcgg tgttcatcgg cccgccgccg gctgcccagt
     8461 cctatctggt gatcgacaag atcattgctg cctgcaagtc gaccggggcc gaggcggtgc
     8521 atccgggcta tggttttctg tccgagcgtg aagccttcgc cgaggcgctg aaggcggcga
     8581 aaatcacttt catcgggcca aaccccaagg cgatcgccgc catgggtgac aagatcgaat
     8641 cgaagaaatt cgccaatgcc gccaaggtct cgactgtgcc gggttatctc ggcgtgatcg
     8701 aggggccgga gcaggcggtc aggatcgccg acgagatcgg ctatccggtg atgatcaagg
     8761 cctccgccgg cggtggcggc aagggcatgc gcatcgccta cagccgcgat gaggtggccg
     8821 acggcttcgt ccgcgccaag aacgaggcag cggcgagctt cggtgacgac cgggttttcg
     8881 tcgagaaatt catcgtcgat ccgcgccata tcgagatcca gctgatcggc gacaagcatg
     8941 gcaatgtcgt ctatctgaac gagcgcgaat gctcgatcca gcgccgcaac cagaaggtga
     9001 tcgaggaggc gccgtcgccg ctgctcgacg ccgccacccg caagaaaatg ggcgagcagg
     9061 cggtggcgct ggcaaaagcc gtcaattatg acagcgccgg cacggtggaa ttcgtcgccg
     9121 ggcaggacaa gtcattcttc ttcctcgaaa tgaacacccg cctgcaggtc gagcatccgg
     9181 tgacggaact gatcaccggc atcgacctcg ttgaggagat gatccgtgtc gccgccggcg
     9241 agaagctgcg gttcaagcag tcggatgtcg gcatcaacgg ctgggcggtc gagagccgcg
     9301 tctatgccga ggacccggtg cgcaatttcc tgccgtccac cggccgcctg atcaagtatc
     9361 gcccgccgac ggaagggacc ttcggcgatg tcaccgtgcg caacgatacc ggcgtctacg
     9421 agggcggtga aatctcggtc tattacgatc cgatgatcgc caagctggtc acccatgcgc
     9481 caacccggct gggcgcgatc aaggcgcagg ccgaggcgct cgatgccttt gccatcgagg
     9541 gtatccgcca caatatcccg ttcgtttcgg cgctgatgca tcatccgcgc tggcagaagg
     9601 gcgcgctgtc gaccgggttc atcaaggaag agtttcccga tggattcgtg gcgccggcgc
     9661 ccgagggcga ggttgccctg cggatggcgg cggtcgcggc ctggcttgat catacccaca
     9721 atgtccgtcg ccggctgatc gaccaccaga tggcgaccgg caagaagttc gagttctcgc
     9781 gcgagcggtc ggtgatgctc ggcaagcagc gtatcaacct gctggtgcat ggcgcggccg
     9841 atggactggt tatcgagttt gccgatacgg ggcgcaaggt cgaggtggtg gccgaaacgc
     9901 agatcggcga tccgctgtgg caggggacga tcggcaatga ccgggtgtcg gcccaggtgc
     9961 ggccggtgct caacggttac tggctggccc atcacggcac cgaggcggta gcgctggtct
    10021 ttacccgtcg cgaagccgat ctgtgggcgc tgatgccgga gaaggttgca gcggacactt
    10081 cgaagaagct gctgtgtccg atgccgggcc tggtcaagca ggttgcggtg atcgaagggc
    10141 aggaggtcaa ggccggcgat attctcgcgg tggtcgaagc catgaagatg gagaacgtgc
    10201 tgcgcgccga gcgcgacgtg accgtcaagg cgatcaaggc cagggccggc gacagtctcg
    10261 cggtcgatgc cgtgatcatg gaatttgcct gacactcaat cgggcggcgg ccatgtaacc
    10321 tgcctgtccg cccgtgctcg catcgtctcg atcaggcgcg cgttggcttc gtccgagcgc
    10381 agcacccatt cagtcgccgc ctgcctgctg cggccgaagc tcatatgccg cgcgatcagg
    10441 cgctggatgc gatcctcgac ccgaatcccg acataccagc aggcatcgag cagcggtctt
    10501 acgccggacc aggcgccgtc gcacagaaga taattgccct cggtgatcac cagccgatgc
    10561 tcgggccgta cggcaatggc gccggcgatc ggttcctcaa tctcgcggcg gaaagcgggc
    10621 gcatagacgg tttcgtccgt gccggcagcg gcgcggatgc gggcgagcat ggcggtgtaa
    10681 ccggctgcat cgaaggtatc cggggcgcct ttgcggt
//
LOCUS       DAOMXG010000077        10670 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_3296_length_10670_cov_10.213943, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000077 DAOMXG010000000
VERSION     DAOMXG010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 10670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10670
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3296_length_10670_cov_10.213943"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..642
                     /locus_tag="PLQ11_06750"
     CDS             97..642
                     /locus_tag="PLQ11_06750"
                     /inference="COORDINATES: protein motif:HMM:NF019416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HRK24635.1"
                     /translation="MPVTRRFRKGDADSEALYSPCGAYRYLLTRRWGEGGLLLYVMLN
                     PSTATEERNDPTIERCERRARMLRHGGLAVANLFGFRATYPADLRAAPDPVGPENDAV
                     LMQAAANHDRSGRSAALSLPGRWPVPKFLITGADVMASGVTRGPEVGRRLAEIEARWI
                     AAGMPEGREAQTALLSGFKAP"
     gene            complement(629..1120)
                     /locus_tag="PLQ11_06755"
     CDS             complement(629..1120)
                     /locus_tag="PLQ11_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24636.1"
                     /translation="MAVINRRLVLLAPLLLALPATAQQRWARYTNPKYGTSAEYPADL
                     MQADAEPDARDGRTFRTPDGAALAIFGQFNVLSATPRSLEQDKRGEDDYREITYSSRG
                     PNWFVLSGFRELEDGRHVFYEKYQFSADGGTIHALVLVYPETEKPRIDPLVRRIAASL
                     KGP"
     gene            complement(1138..1884)
                     /locus_tag="PLQ11_06760"
     CDS             complement(1138..1884)
                     /locus_tag="PLQ11_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HRK24637.1"
                     /translation="MKSRWLPLVASLLAAGPVLAEPSALDSLVAAYPAVLAGHDGTHL
                     MFRDGTRLAVSDGRTGKSFDEKLRSASILDQMSLPYPKGPLTGPPGPQDDPGRFRNQA
                     FFDKIYGDCSRGGVRKSLVRVPWLPKSGGGTVEITSINGVADRLRAVSEDLDRLPLEI
                     RKFAFPSAGTFNCRTVKDTGARSMHAWGAAIDLNTRYSDYWLWSKGKGYRNRIPFEIV
                     AVFERHGFIWGGKWGHYDTMHFEYRPELLQ"
     gene            2056..2370
                     /locus_tag="PLQ11_06765"
     CDS             2056..2370
                     /locus_tag="PLQ11_06765"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRK24638.1"
                     /translation="MKRPVARLAGAAMAVVAMTPGLASTFCPIPNTRDGFVALRAGPD
                     LKAPIVARMRQGDEIQAMSEQSGPWIRARYWRGDERLTRGFGRHRATGWVHRSLVADL
                     CG"
     gene            complement(2374..3261)
                     /gene="hemF"
                     /locus_tag="PLQ11_06770"
     CDS             complement(2374..3261)
                     /gene="hemF"
                     /locus_tag="PLQ11_06770"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HRK24639.1"
                     /translation="MTLPDTQLRPKAWFEQLQTRICASFEALEDQCPGPFFAEAPAEA
                     GRFEKRPWQRTNHDGAPGGGGTMGLMRGRVFEKVGVHVSTVFGTFAPEFAGQIPGASE
                     DPRFWASGISLIAHPWNPNVPTVHMNTRRVVTTKQWFGGGADLTPVLDRRRTQEDADS
                     VAFHAAMRAVCDGHAAVAPYEKFRNWCDEYFYLKHRKEPRGIGGIFYDYLDTGDFEAD
                     FAFTRAVGEAFDAIYPAIVRANWDRHWTEADRHEQQVRRGRYVEFNLLYDRGTIFGLK
                     TGGNVDSILSSMPPTVRWP"
     gene            complement(3258..3770)
                     /locus_tag="PLQ11_06775"
     CDS             complement(3258..3770)
                     /locus_tag="PLQ11_06775"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018042310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HRK24640.1"
                     /translation="MTHHDSSESCTPPTPVMLALYQPDIPQNTGTIIRMAACLGVPVG
                     IIEPAGFPASDRAFRRAGMDYLDAAEIRRHASFAAFQQWRSAAGARLLLATTRAADDL
                     YAVRFRPGDVILFGRESLGVPQDVHDAADLRFRIPMRPELRSLNIAVSAAMITGEALR
                     QLGAFPKAAP"
     gene            3936..4475
                     /gene="petA"
                     /locus_tag="PLQ11_06780"
     CDS             3936..4475
                     /gene="petA"
                     /locus_tag="PLQ11_06780"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HRK24641.1"
                     /translation="MAEHAIDDTNRRDFLYIWTGALGAVAAGSAVIPLVDQMNPDAAT
                     IAAGAPIEVDLTQIPEGQVVTLKWRGKPIFVRHRSKKEIDESKAVAVASLPDPQPDSA
                     RVGSFNGKPMDKWIVNIGSCTHLGCVPISNSGEFHGWYCPCHGSHYDTAGRIRKGPAP
                     KNLELPPFSFQSESKVRIG"
     gene            4502..5806
                     /locus_tag="PLQ11_06785"
     CDS             4502..5806
                     /locus_tag="PLQ11_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006937653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6"
                     /protein_id="HRK24642.1"
                     /translation="MSDHSTYTPKTGIERWIDARMPIIRVMHDAGVSYPTPKNLNYLW
                     TFGGILTFMLVAQIVTGIVLVMHYTPHTTMAFNSVEHIMRDVNYGWLLRYFHANGASM
                     FFIAVYIHMFRGLYYGSYKAPRELLWILGVVIYLLMMATGFMGYVLPWGQMSFWGATV
                     ITNLFSAFPIVGEPIVTWLWGGYSVDNPTLNRFFSLHYLLPFMLAAVVFLHIWALHVV
                     GQNNPDGVEVKNFAKDTVPFTPYATAKDAFAMVCFIVFYGYFAFFMPNYLGHPDNYIQ
                     ADPLVTPAHIVPEWYYLPFYAILRSIPDKLMGVIAMFAAIAILAFLPWLDTSRVRSTR
                     YRPTYKIFFWTLVVVCFLLGYCGSQPTDKVVLSLGGTKILDIAGFGAILTAYYFLHFI
                     VVLPLLGLFEKTRPEPASIADAVLAKHGHAAPAGAVHAPEKR"
     gene            5871..6719
                     /locus_tag="PLQ11_06790"
     CDS             5871..6719
                     /locus_tag="PLQ11_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014286754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HRK24643.1"
                     /translation="MFRSLTPRRIAAAVLASGLIAGAASIAHAAGGDTPKPEKQSWSF
                     SGPFGTFDRAQLQRGFKVYKEVCANCHSLNLVAFRNLSDKGGPEFSVDQVKALAATYQ
                     IKDGPNDKGEMFERPGRPADKFPSPFPNPEAAKASLGGAPPDLSLIAKARSYERGVVL
                     SLVDLVTQYQEHGPDYLVALLAGYSDPPAGVTVGSGLAYNKYFPGGQLSMAQPLNPDQ
                     VEYTDGTKGTVEQYAKDVTAFLMWAAEPKMEDRKRIGAFFMMLMITLAFFTYFTKKKV
                     WSRVQH"
     gene            complement(6856..8625)
                     /locus_tag="PLQ11_06795"
     CDS             complement(6856..8625)
                     /locus_tag="PLQ11_06795"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRK24644.1"
                     /translation="MAETPVDRLQDLILFLGTAGIVVPLFKRLNLSPTLGFLAAGILL
                     GPYAIGQLAQSVPVLKHISIANPDGIAHVGEFGVVFLFFMIGLELSLERVRILRRLIF
                     GFGASQFLLSTLAIGFVAWLLGQQPVAAFVIGAALAMSSTAIVVPTLAERKKLATPAG
                     RGIFAVLLFQDLAVTPLLVMVTLLASAKEGAGSGAALLTLMQAGIGLVLLFFVGRIVL
                     RPLFHSVALARSQEFFTAACLLVVLGAGLVAQMSSLSMAIGAFIGGLLLAETEFRRQI
                     EATIDPFRGLLLGLFFIAIGARLDLNLLFDNPGQILAITAALIGLKALVLYLIMPRFH
                     LPDKGRHDTAWLLAPAGEFGFVLLAAAMSSGLLWPSLGGRLMVAITLSMFLIPVFAML
                     TDRMTRRPAVEPAPFDEIDDTTPSVIVIGCGRVGELVLEMLAHHKVPAFAVESSQRRV
                     AMARDKGLRAYYGDPQVPQFFDRLPLKTCRALVLTMNTATLTENLARSARAHYPDLTI
                     VARAHDPSHAERLYELGVTDAVPETFEASLHLAEATLVDIGVPMGLVIASVHEKREIT
                     RNQLRAAGYQVRDPRSVRRFVKD"
     gene            8795..9892
                     /gene="ychF"
                     /locus_tag="PLQ11_06800"
     CDS             8795..9892
                     /gene="ychF"
                     /locus_tag="PLQ11_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HRK24645.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTQTAAAQAANYPFCTIEPNVGD
                     VAVPDERLAALAAIAGSRQIIPTRLTFVDIAGLVRGASKGEGLGNQFLANIREVDAIA
                     HVVRCFEDGDVTHVEGRIDPIADIETIETELMLADLDSLEKRLVALEKKARGGDKEAK
                     EALELITAALGLLREGKPARLVERSAEQERAFQSLGLLTSKPVLYVCNVEEASADKGN
                     AHSEKVMARAREEGARAVVVSAKIESEIAVMAADDQAEFLEAVGLSEPGLNRVIREGY
                     ALLGLITYFTVGPKEARAWTVAEGTRAPQAAGVIHTDFEKGFIRAETIAYADYVGLKG
                     EAGAREAGKFRLEGKEYVVADGDVLHFRFAN"
     gene            9901..>10670
                     /locus_tag="PLQ11_06805"
     CDS             9901..>10670
                     /locus_tag="PLQ11_06805"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HRK24646.1"
                     /translation="MPRFCFEDFVPGTVIGYGDKRVDKDEMIAFARIFDPQPFHIDEE
                     AARSSFSGTLIASGWHTAAMHMRMMCDAFMLDSSSMGSPGVQELKWLRPVQPGDRLSA
                     RRTVLGARASTSKPDRGLVAFAFHVLNQEGHAVLEQKNTILFGLREPGRVPAHAGGLP
                     APDLMPHPGAAAGPQQVDARPGLPFFEDIVVGSVLELGALTFTEADIIAYASDFDPQY
                     FHVDPVAAQKSPFGRLIASGWHTAAGWMRSMAGDRARR"
BASE COUNT         1945 a         3319 c         3520 g         1886 t
ORIGIN      
        1 gtctcctgcg gcagggccgg gcggtggccg tgggcaccta ccgcgcgggt gagctgcacc
       61 cgctgcgcgt cttcaatcag ggctaggcgg cccgccgtgc cggtcacccg ccgcttccgc
      121 aagggcgatg ccgacagcga ggcgctgtat tccccctgcg gcgcctaccg ttatctgctc
      181 acccggcgct ggggcgaggg cggactgctg ctttatgtca tgctgaaccc ctcgaccgcg
      241 accgaggaac gcaacgaccc caccatcgaa cggtgcgagc ggcgggcgcg gatgctgcgc
      301 catggcggcc tggccgtggc gaaccttttc ggctttcgtg ccacctatcc ggccgacttg
      361 cgcgcggcgc ccgatccggt cgggccagag aatgacgcgg tgctgatgca ggcggcggca
      421 aaccacgacc gcagcggccg atcggctgcg ttgtcgttgc cgggccggtg gccggtgccg
      481 aaattcctga tcaccggcgc ggacgtcatg gccagcggcg tgaccagagg cccggaggtg
      541 ggccggcggc tggcagagat cgaggcgcgc tggatcgctg ccggcatgcc cgagggacgg
      601 gaggcgcaga ccgccctgct gagcggattc aaggcccctt gagcgaggcg gcgatgcggc
      661 ggaccaaagg gtctatccgg ggcttttcgg tctcgggata gaccagaacc agcgcatgga
      721 tcgtgccgcc atcggcggag aactgatact tttcgtaaaa gacgtggcgc ccgtcctcaa
      781 gctcgcggaa gccggacagg acgaaccagt tgggtccgcg gctggaatag gtgatctcgc
      841 ggtagtcatc ctcgccgcgc ttgtcctgtt cgagcgagcg cggggtggcg ctcaggacgt
      901 tgaactggcc gaagatcgcc agagcggcgc cgtctggggt gcggaaggtg cgaccgtcgc
      961 gggcatccgg ttcggcgtct gcctgcatca ggtctgcagg atactccgcc gaggtgccgt
     1021 atttggggtt ggtgtagcgg gcccagcgct gctgcgcggt ggcgggaaga gccagcagca
     1081 agggcgccag cagcaccagg cggcggttga tcacggccat ggatggcctc cctctggtca
     1141 ttggagaagt tccgggcggt attcgaagtg catcgtgtcg taatggcccc atttgccgcc
     1201 ccagatgaac ccgtggcgct cgaacaccgc gacgatctcg aacgggatgc ggttgcggta
     1261 gcctttgccc ttgctccaca gccagtaatc cgaatagcgc gtgttgaggt cgatggcggc
     1321 gccccaggca tgcatcgagc gggcgccggt atccttcacg gtgcggcaat tgaaggtgcc
     1381 ggcgctggga aaggcgaatt tgcggatttc cagcggcagg cggtcgagat cctcggagac
     1441 cgcgcgcagc cggtcggcga cgccgttgat cgaagtgatc tcaaccgttc ccccgccgga
     1501 tttcggcagc cagggcacgc gcaccagcga cttgcgcacg ccgcccctgg aacagtcgcc
     1561 gtagatcttg tcgaagaatg cctgattgcg gaagcgaccg ggatcgtcct gcggtccggg
     1621 cgggccggtc agcggccctt tcggataggg cagcgacatc tggtcgagga tcgaggcgga
     1681 gcgcagcttc tcgtcgaaac tcttgccagt acggccatcg gaaacggcaa ggcgcgtccc
     1741 gtccctgaac atgagatggg tgccgtcatg cccggcaagc accgccggat aggcagcaac
     1801 cagcgaatcg agcgcggagg gctctgccag aaccggtccg gcagccaaaa gcgaggcgac
     1861 gagcggcaac caacgcgact tcacggcgat ctccccgata tcaggaaatc agcttccccc
     1921 attatctgga acagcggcac cgcatgccat gagccagcgc aaaggcgtgg caggactcta
     1981 tcgggcggac gagtcttttc atgttttcgg ccgaggctgt cgtgatatgc tggccacctt
     2041 gagcaggggg ctgcgatgaa gcgtccggtc gcacggctgg cgggggcggc gatggccgtc
     2101 gtggcgatga cgcctgggct ggcatcgaca ttctgcccca ttccgaacac cagggacggg
     2161 ttcgtcgcgc tcagggccgg acccgacctg aaggcgccga ttgtcgcccg gatgcgtcag
     2221 ggcgacgaaa tccaggcgat gtccgagcaa tcggggccct ggatccgggc gcgctactgg
     2281 cgtggcgacg aacggctgac gcgaggcttc ggcaggcatc gcgccacggg atgggtgcac
     2341 aggagcctgg tggccgatct ctgcggctga gtgtcacggc caccgcaccg tcggcggcat
     2401 cgacgacagg atcgaatcga cattgccgcc ggttttcagg ccgaagatgg tgccgcggtc
     2461 gtagagcagg ttgaattcga catatcggcc gcggcgcacc tgctgttcgt ggcggtcggc
     2521 ctcggtccaa tgcctgtccc agttggcgcg gacgatggcc ggatagatcg catcgaaagc
     2581 ctcgcccaca gcgcgggtga aggcgaaatc ggcctcgaaa tcgccggtgt cgaggtaatc
     2641 gtagaaaatg ccgccgatgc cgcgcggctc cttgcgatgc ttgaggtaga aatactcgtc
     2701 gcaccagttc ctgaatttct cgtaaggggc gaccgcggca tggccatcgc agacggcgcg
     2761 catggcggca tggaaggcga ccgaatcggc atcctcctgc gtgcggcgcc ggtcaagcac
     2821 cggggtgagg tcggcgccgc cgccgaacca ttgcttcgtg gtcaccacgc ggcgggtgtt
     2881 catgtgcacg gtcggcacgt tcggattcca gggatgggcg atcagcgaga tgcccgaagc
     2941 ccagaagcgc ggatcctcgg aggcgccggg aatctggccg gcgaattcgg gggcaaaggt
     3001 gccgaatacg gtcgagacat gcacgccgac cttctcgaat acgcggccgc gcatcagccc
     3061 catggtgccg ccgccgccgg gtgctccgtc gtggttggtg cgctgccacg gccttttctc
     3121 aaatcgcccc gcttctgccg gtgcttcggc aaaaaacggg ccggggcact ggtcctccag
     3181 tgcctcgaat gaggcacaga tgcgggtttg cagctgttca aaccaggctt tggggcggag
     3241 ctgggtgtcg ggcagggtca tggtgcagct ttcggaaaag cgccgagctg gcgcagggcc
     3301 tcgccggtga tcatggcggc gctgacggcg atgttcaacg agcgaagttc ggggcgcatc
     3361 ggaatgcgga agcgcagatc ggcggcgtca tgcacatcct gcggcacgcc aaggctttcg
     3421 cggccgaaga ggatcacgtc gccgggccgg aagcggacgg cgtagagatc atccgctgcc
     3481 ctggtggtgg ccagcagcaa ccgtgcgccc gcagcgctgc gccattgctg gaatgccgca
     3541 aaactggcat ggcgacggat ctccgccgca tcaagataat ccatgccggc ccggcgaaag
     3601 gcccgatcgc tggccggaaa ccccgccggt tcgatgatcc ccaccggcac acccaggcag
     3661 gcggccatgc ggatgatcgt tccggtgttc tgcgggatat ccggctgata gagcgccagc
     3721 atcaccggtg tcggcggcgt gcagctttcg gaactgtcgt gatgcgtcat cttggctatc
     3781 taatcgctga aaggactatg gacaatgcga ttgcagcctg ccaaagagca tgctgcggat
     3841 gacgtgcggc gcgagcctcc tgtcacgaca gacattgcag aaatggtcga gcggacggcg
     3901 cggcgccgct gctccacagg ctgagggtgc ataaagtggc tgaacacgcg attgacgaca
     3961 cgaaccggcg cgatttcctt tacatctgga ccggggccct gggcgcagta gcggccggct
     4021 cggcggtcat tccgctggtt gaccagatga atccggacgc tgccaccatc gctgccggcg
     4081 cgccgatcga agtcgatctg acgcagattc ccgaggggca ggtggtgacg ctgaagtggc
     4141 gcggcaagcc gatcttcgtc cgccaccggt ccaagaagga aatcgacgaa tcgaaggccg
     4201 tggccgtggc cagcctgccc gacccgcagc ctgacagcgc gcgcgtcggc tccttcaacg
     4261 gcaagccgat ggacaagtgg atcgtcaata tcggcagctg cacgcatctg ggctgtgtgc
     4321 cgatctccaa ctccggcgag ttccatggct ggtattgccc ctgccacggc tcgcattacg
     4381 acaccgccgg ccgcatccgc aagggcccgg cgccgaagaa cctggaactg ccgcccttct
     4441 ctttccagtc ggaaagcaag gtccgcatcg gctaatccac tgatcctcgc aaggtaattc
     4501 catgtccgac cattcaacct acacacccaa gacgggcatc gagcgctgga tcgatgcgcg
     4561 tatgcccatc atccgggtga tgcacgacgc cggcgtctcc tatccgacac cgaagaacct
     4621 caattatctt tggacgttcg gcggcatcct gaccttcatg ctggtggccc agatcgtcac
     4681 cggaatcgtc ctggtgatgc actacactcc gcacaccacg atggcgttca attcggtcga
     4741 gcatatcatg cgtgatgtga actacggctg gctgctgcgc tatttccacg ccaacggcgc
     4801 gtcgatgttc ttcatcgcgg tctatatcca catgttccgc ggtctctatt acggctctta
     4861 caaggcgccg cgcgagcttt tgtggatcct cggcgtggtc atctaccttt tgatgatggc
     4921 gaccggcttc atgggttatg tgctgccttg gggccagatg tcgttctggg gcgccactgt
     4981 catcaccaac ctgttctcgg ccttcccgat tgtcggcgag ccgatcgtga cctggctgtg
     5041 gggcggctat tcggtcgata atccgacgct gaaccgcttc ttctcgctgc attacctgct
     5101 gccgttcatg ctcgccgccg ttgtgttcct gcacatctgg gcgctgcatg tggtcggcca
     5161 gaacaacccc gacggcgtcg aggtgaagaa ttttgccaag gacaccgtgc cgttcacgcc
     5221 gtatgcgacc gccaaggacg cgttcgcgat ggtctgcttc atcgtgttct acggctactt
     5281 cgccttcttc atgccgaact acctggggca cccggacaat tacatccagg ctgatccgct
     5341 ggtgacgccg gcccatatcg ttccggaatg gtattacctg ccgttctacg cgatcctgcg
     5401 gtcgatcccg gacaagctga tgggggtcat cgccatgttc gcagcgatcg cgatcctggc
     5461 gttcctgccg tggctggata ccagccgtgt ccgctccacg cgctatcggc cgacctacaa
     5521 gatcttcttc tggacgctgg tggtggtgtg cttcctgctc ggatactgcg gctcgcagcc
     5581 gaccgacaag gtggttctgt cgctgggcgg aaccaagatc ctcgacatcg ccggattcgg
     5641 cgcgatcctg accgcttatt atttcctgca cttcatcgtg gttctgccgc tgctcggcct
     5701 gttcgagaaa accaggcccg agccagcatc gattgccgat gctgtgctgg ccaagcacgg
     5761 acatgccgcg ccggccggtg cggtccatgc tccggaaaag cgctgaaacg aaccgaacgg
     5821 acaggcccgg cgtctggccg ggcagtaaca tgacggacga aggaacacag atgtttcgtt
     5881 cactcacccc tcgccggatt gccgccgcag tgctcgcttc cggcctgatc gccggtgcag
     5941 cctccatcgc ccatgcggcc gggggcgata cgcccaagcc ggaaaagcag agctggtcgt
     6001 tctccggacc gttcggcacg ttcgaccgtg cccagttgca gcgcggcttc aaggtctaca
     6061 aggaagtctg cgccaattgc cattcgctca acctggttgc cttccgtaac ctgtccgaca
     6121 agggcgggcc ggaattctcc gtcgatcagg tgaaggcgct ggccgccacc taccagatca
     6181 aggacggccc caacgacaag ggcgagatgt tcgagcggcc gggccggccg gcggacaagt
     6241 tcccgtcgcc cttcccgaac ccggaggccg ccaaggcttc gctcggtggc gctccgccgg
     6301 atctgtcgct gatcgccaag gcgcgctcct atgagcgtgg ggtggtcctg tcgctggtcg
     6361 atctggtgac ccagtatcag gagcacgggc cggattatct ggtggcgctg ctggctggct
     6421 attccgatcc gccggccggg gtcacggtcg gaagcggcct tgcctacaac aagtatttcc
     6481 ctggtgggca attgtcgatg gcccagccgc tcaatcccga tcaggtcgaa tataccgatg
     6541 ggaccaaggg cacggtcgag caatacgcca aggacgtcac cgctttcctg atgtgggcgg
     6601 cagagccgaa gatggaagat cgcaagcgga tcggcgcctt cttcatgatg ctgatgatca
     6661 cgctggcctt cttcacctac tttaccaaga agaaggtctg gtcgcgcgtc cagcattgac
     6721 gggacgtgcc ggggcgcggc gtcaagcccg cccgttcagc cgggattgtc gggaaagggg
     6781 gccgagaggc cccttttgct ttgtccgcag ccttgtcccg caggatgcaa acgtccggtg
     6841 ccggccaaaa aaccgtcaat ccttcacgaa gcgcctgacg ctcctgggat cacgcacctg
     6901 ataaccggca gcgcgcaact ggttgcgggt gatctcgcgc ttttcatgga ccgaggcgat
     6961 gaccagcccc atcggcacgc cgatatccac cagcgtcgcc tcggccagat gcaggctcgc
     7021 ctcgaaggtt tccggcaccg catcggtgac gccgagttca tacagacgct cggcatgcga
     7081 gggatcatgg gcgcgggcaa cgatggtcag atcaggataa tgggcgcggg cgctgcgtgc
     7141 cagattttcg gtcaaggtcg cggtattcat ggtcaggacc agcgcccgac aggttttcag
     7201 cggcagccga tcgaagaatt gcggcacctg ggggtcgccg taataggcgc gcaacccctt
     7261 gtcgcgtgcc atggcgaccc gccgctggct tgattccacc gcaaaggccg ggaccttgtg
     7321 atgggccagc atttccagaa ccaattcgcc gacgcggccg cagccaatga cgatgaccga
     7381 cggagtggtg tcgtcgatct cgtcgaacgg cgccggttcg accgccggcc ggcgggtcat
     7441 ccggtcggtc agcatggcga aaaccgggat caggaacatc gacagcgtaa tggcgaccat
     7501 caggcgcccg cccagcgagg gccaaagcag gcccgaactc atcgccgccg ccagcagcac
     7561 gaagccgaat tccccggcag gggcgagcag ccaggcggtg tcgtggcgcc ccttatccgg
     7621 caggtggaac cgcggcatga tgagatagag caccagcgcc ttgagcccga tcaacgccgc
     7681 tgtaatggcg aggatctggc cgggattgtc gaacagcagg ttgagatcga gccgggcgcc
     7741 gatggcaatg aagaacaggc cgagcagcag accacggaac ggatcaatcg tcgcctcgat
     7801 ctgccggcga aactcggttt cggccagcaa caacccgccg atgaaggcgc cgatggccat
     7861 cgacaggctg ctcatctgcg ccaccaggcc ggcaccgaga accaccagca ggcacgcggc
     7921 ggtgaaaaac tcctggctgc gggccagggc caccgaatgg aacagcggcc tgagcacaat
     7981 gcgcccgacg aaaaacagca aaaccagccc gatgcccgcc tgcatcaatg tcaatagcgc
     8041 cgcgccgctg ccggcgcctt ccttggcact ggccagtagc gtgaccatca ccagcaacgg
     8101 ggttaccgca aggtcctgaa acagcagcac ggcaaaaatg ccgcgcccgg ccggtgtcgc
     8161 cagcttcttg cgctcggcca gggtcggcac gacgatggcc gtcgaggaca tggccagcgc
     8221 cgcgccaatg acaaaggcgg ctacgggctg ctggcctaac agccaggcga caaagccgat
     8281 cgccagggtg ctgagcagga actggctggc gccgaagccg aagatcagcc ggcgcaggat
     8341 gcgcacacgc tccagcgaca gttcaagccc gatcatgaaa aacaggaaca cgacgccgaa
     8401 ttccccgaca tgggcaatac cgtcgggatt ggcgatggat atgtgcttga gcaccggaac
     8461 actctgggcc agctggccga tggcataggg gcccagcagg atgccagccg ccagaaagcc
     8521 gagcgtcggc gacagattga gccgcttgaa cagcggcacg acgatgcccg ctgttcccag
     8581 gaacaggatc agatcctgaa ggcgatcgac cggcgtctcg gccatggaac ctcgttgcac
     8641 ttgtgttggc aggtgactcg tctgtctgag tgtagcgcat ggctcgccgg caatagaagg
     8701 gcatctgcgg tttcggaatg cgattttgag gttgcagttt ggtccgaacc gccgtttatc
     8761 ggtgccaatc gacgcagctt cgaaagacgc ctccatgggt ttcaaatgtg gtatcgtcgg
     8821 cctgccgaat gtcggcaagt cgacgctgtt caatgcgctc acgcagaccg cagccgcgca
     8881 ggcggccaat tatccgttct gcacgatcga gccgaatgtc ggcgacgttg ccgtgccaga
     8941 cgagcggctg gcagcactgg cggcaattgc aggctcgcgc cagatcattc cgacgcggct
     9001 gaccttcgtc gatattgccg ggctggtgcg cggcgcctcc aagggcgaag ggctcggcaa
     9061 ccagttcctc gccaacatcc gcgaggtgga tgccatcgcc catgtggtgc ggtgcttcga
     9121 ggatggtgat gtcacgcatg tcgagggccg tatcgacccc atcgccgata tcgagaccat
     9181 cgagaccgaa ttgatgctgg ccgatcttga cagcctcgaa aagcgcttgg tcgcgctgga
     9241 aaagaaggcg cgcggcggcg acaaggaggc caaggaggcg cttgagctga tcacggccgc
     9301 gctcggtctg ctgcgcgagg gcaagccggc gcgtctggtc gagcggtcgg cagagcagga
     9361 gcgggcgttc cagtcgcttg gcctgctcac ctccaagccg gtgctgtatg tctgcaatgt
     9421 cgaggaagcc agcgccgaca agggcaacgc ccattcggaa aaggtgatgg cgcgggcgcg
     9481 cgaggaaggc gcgcgggccg tagtcgtctc ggccaagatc gaaagcgaaa tcgcggtcat
     9541 ggcggccgat gatcaggccg agttccttga ggctgtcggg ttgagcgagc cggggctcaa
     9601 ccgggtcatc cgcgaaggct acgcgcttct ggggctgatc acctacttta ctgtcgggcc
     9661 gaaggaagca cgcgcctgga cggtggcaga gggtacgcgc gcgccccagg cggccggtgt
     9721 gatccatacc gatttcgaaa aaggcttcat ccgcgccgaa accatcgcct atgccgatta
     9781 tgtcgggctc aagggcgagg ctggtgcgcg cgaggcagga aaattcaggc tggaaggcaa
     9841 ggaatatgtg gtcgccgacg gcgatgtcct gcatttccgc ttcgccaact gaggtcagcc
     9901 atgccacgtt tctgctttga ggacttcgtg cccggcacgg tgatcggcta tggcgacaag
     9961 cgcgtcgaca aggacgagat gatcgctttt gcgcgcatat tcgatccgca gccgttccac
    10021 atcgacgagg aggcggcgcg gtcaagtttt tcgggaaccc tgatcgcctc gggctggcac
    10081 acggctgcga tgcacatgcg gatgatgtgc gatgcgttca tgctcgattc ctcatccatg
    10141 ggttcgcccg gggtgcagga actcaaatgg ctccgcccgg tgcagcccgg agatcggctg
    10201 agcgcccgcc gcactgtgct gggagcgcgg gcttcaacct ccaagccgga tcgcgggctg
    10261 gtcgcctttg cctttcatgt gctcaatcag gaaggacatg cagtcctgga gcagaagaac
    10321 acgattctgt tcggtctgcg cgagccgggc cgggtccctg cccatgccgg cggcctgccg
    10381 gcgccggatc tgatgccgca tcccggcgcg gctgcaggcc cccagcaggt cgatgcccgc
    10441 cccggcctgc cattcttcga agacatcgtc gtggggagcg ttctggagct tggcgcgctg
    10501 accttcaccg aagccgatat cattgcctat gccagcgact tcgatccgca atatttccac
    10561 gtcgatcctg tcgcggcaca gaaaagtccg ttcggccggc tgatcgcttc gggctggcat
    10621 actgcggcgg gctggatgcg ttcgatggcc ggtgaccggg cgcggcgcat
//
LOCUS       DAOMXG010000078        10550 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_3357_length_10550_cov_10.180753, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000078 DAOMXG010000000
VERSION     DAOMXG010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 10550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10550
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3357_length_10550_cov_10.180753"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            511..1293
                     /gene="hisN"
                     /locus_tag="PLQ11_06810"
     CDS             511..1293
                     /gene="hisN"
                     /locus_tag="PLQ11_06810"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HRK24647.1"
                     /translation="MRPVDLVEFLDHLALLSGEAILPFFRNHISVTDKAGKGVFDPVT
                     EADRAAELVIRREIKQRFPQHGILGEEFGGDHEEAEFVWVVDPIDGTRSFICGLPVWG
                     TLIGLKRFDAAVLGLMHQPYTRERFFGDGARAWLKGPSGERKLSTRATTDLAEATLMT
                     TTPALFKGSMREAYDRVENTVRLARYGTDCYAYAMLASGHVDLVIETGLKAYDIVALI
                     PIIEGAGGVITDFSGGSVINGGDVVVAANHQLHEKALKLLNR"
     gene            complement(1283..2293)
                     /locus_tag="PLQ11_06815"
     CDS             complement(1283..2293)
                     /locus_tag="PLQ11_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRK24648.1"
                     /translation="MRDALNRLYSIDANPAPPGAEVVRLTTTDGLALRAAVFRPANPR
                     ASVVILQGRSEYIERYFETIGELMQRGFAVVAFDWRGQGGSMRELSDPMKGHVDDFLL
                     YLRDLDAVLTFIGASDLPRPCFGLAHSMGGAVLALALNHGEQRLARAVVTAPMLGIYH
                     APTGGWGHIAARLLNVVGLGGQYVPVTSPQTATAFAPFEDNVLTSDPERHERSVAILT
                     AAPELAVGAPTISWVAAAFRLIGDFNNPEFGLRFACPLLLVLAGGDRVVDTPAAERFA
                     LRARGASTVTLTGAQHEILMERDVFRAEFWAAFDAFVPHCQPGEEPGLSAETSDASPV
                     SG"
     gene            complement(2354..2782)
                     /locus_tag="PLQ11_06820"
     CDS             complement(2354..2782)
                     /locus_tag="PLQ11_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24649.1"
                     /translation="MLSKLSPTLGIAWPRRPAGRLSRAMLLVIAGGLAAAIWLAVGSW
                     SPKLPALSALKDPSHASLPANRGLSGEAVTRRLMQAGYLDVHDVRRRGAVYLARARIS
                     DRQGVRLVVHAQSGEVLGLKTVIERGSGGPQRAAEVLAGP"
     gene            2998..3450
                     /locus_tag="PLQ11_06825"
     CDS             2998..3450
                     /locus_tag="PLQ11_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HRK24650.1"
                     /translation="MRHFDLTPLYRSTIGFDRLFSLFDDLPNLDSSGGYPRYNIEKTG
                     EHDYRITLAVAGFGEADLAVEVADGVLIVRGTRQEKDGEARPEMLFQGIAARAFERRF
                     QLADSVTVAGARLENGLLSVDLKREIPEARKPRMIPIGHGAKPVEIAH"
     gene            complement(3513..4316)
                     /locus_tag="PLQ11_06830"
     CDS             complement(3513..4316)
                     /locus_tag="PLQ11_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24651.1"
                     /translation="MMRSRTIPARPLVRLLPLALVLALAAPVASPATAQYFGRGFDDD
                     VLSPREVVEILRDRGFSRLTRPLRNRDVYVVNAIDPYGVPVRLIVSSDDGSILEAFRQ
                     RGIPRGSRIEIDPEDDPRVVVPPPRAEPPRATRPAPQRDAVPRSNDAEQAPAPPTSAK
                     RSPLALPKDPAAPGKTDPSAGTRERPRVIPLTPGVTAPSTQAVPQLSPGVQTSQTPVL
                     PDLPNAPPPAVLDPRGPQLAPSQGQGGSLLDFRSGRGGDGSAIPPPPLD"
     gene            complement(4390..5460)
                     /locus_tag="PLQ11_06835"
     CDS             complement(4390..5460)
                     /locus_tag="PLQ11_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HRK24652.1"
                     /translation="MIFTSDNVVGASDKVMAGLAVANSGALASYGADPITQRVEAAFD
                     ALFERKVTSFLVTTGTAANALAIAAMVPPYAAALCHRESHIIDDECGAPEFFAGGAKL
                     VGIAGTGAKLSARAIAAHLAEEPGGTNHPPFRGISLSQATECGMVHRLDEIRAIAETA
                     HAAGMSLHMDGARFANALLTLGCTPAEMTWKAGVDCLSFGGTKNGCLMAEAVIFFNEK
                     LAEDFKFRRKRAGQTVSKGRLLSAQFEAYLDAGHWLDLAAKANGAATRLSLELAAIPG
                     VRLAWPTEANEVFVILKREVIAALRARGAAFYEWTAKSLAPDDRLAEDEDVVRLVCSF
                     ATSSAEVERFIDIVRSNPVQGG"
     gene            5733..10454
                     /gene="gltB"
                     /locus_tag="PLQ11_06840"
     CDS             5733..10454
                     /gene="gltB"
                     /locus_tag="PLQ11_06840"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006469486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HRK24653.1"
                     /translation="MTVRFVNDRAQSHDSGEAQSRTLRVVDPGLPVAEGLYDPALEKD
                     SCGVGFIANMKNVKSHAIVTQGLQILHNLDHRGAVGADPKAGDGCGMLVQIPHRFFKE
                     ECAKLGYDLPEPGHYAILQLFLPRDKAGRRTVQKIVDKVIKAEGQRLIGWRDVPVDNG
                     DLGVSVLPTEPVHRQVFIGRGEGIANEDEFERRLFILRKVISNAVYDLKDPTTKGYYP
                     VSSSCRTVVYKGMLLAGQLGSYFADLNDPRFESALALVHQRFSTNTFPTWSLAHPYRM
                     VAHNGEINTLRGNVNWMAARQASVDSELFGSDISKLWPISYEGQSDTACFDNALEFLV
                     QGGYSLAHAMMMLVPEAWAGNPLMDDQRRAFYEYNAALMEPWDGPAAVAFTDGRQIGA
                     TLDRNGLRPARYFVTDDGLVVMASEMGVLPIPEEKIVTKWRLQPGKMLLIDLGEGRII
                     SDEEIKAELASANPYKDWLKRTQIVLEDLPPVEPRAPRTDVPLLDKQQAFGYSQEDLA
                     LLMAPMATTGQEAVGSMGTDTPISALSDKSKLLYTYFKQNFAQVTNPPIDPIREELVM
                     SLVSFIGPRPNLFDLEGLSRRKRLEVRQPILTNEDLEKIRGIGHFEDRFDTKTLDITY
                     PAERGAGGMQDAIERLCDRAEAAVAGGYNIIILSDRLVGPERVPIPALLATAAVHHHL
                     IRKGLRTSVGLVVETGEAREVHHFCCLAGYGAEAINPYLAFETMEAMHEKGEFPPEVE
                     AKEVVKRYIKSVGKGILKVMSKMGISTYQSYCGAQIFDAVGLGQSFVDKYFFGTASRI
                     GGVGLAEVAIETVMRHRDAFGDNPVLKTSLEVGGEYAFRFRGEAHAWSPTTVAALQHA
                     VRGNSKERYEEFARAINEQNERLLTIRGLFRIKAAAEDGRKPVDVSEVEPANVIVRRF
                     ATGAMSFGSISREAHTTLARAMNALGGKSNTGEGGEESDRFRPLPDGRSNPERSAIKQ
                     VASGRFGVTTEYLVNSDMMQIKMAQGAKPGEGGQLPGHKVDAVIAKVRHSTPGVGLIS
                     PPPHHDIYSIEDLAQLIYDLKNVNPAGDVSVKLVSEVGVGTVAAGVAKARADHVTISG
                     FEGGTGASPLTSIKHAGSPWEIGLAETHQTLVRNRLRSRIAVQVDGGLRTGRDVVVGA
                     LLGADEFGFATAPLIAAGCIMMRKCHLNTCPVGVATQDPVLRKRFIGTPEHVINYFFF
                     VAEEVRQLMAEMGYRTFNEMVGQMQMLDRSEAIAAWKAKGIDFTRLFHKPEEGAETGI
                     FVSERQDHHLEKVLDRQFIAAASPALTDKTRVRAEFKITNVDRTAGAMLSGEVAKAYG
                     HAGLPEDTIHLKLNGTAGQSFGAWVARGVTLELEGEANDYVGKGLSGGKLIIYPSAKA
                     QQIKPEASIIVGNTVLYGAISGECYFRGVAGERFAVRNSGAIAVVEGTGDHGCEYMTG
                     GIVVVLGNTGRNFAAGMSGGIAYVLDEDKTFAKRCNMSMVDLEPVPEEEDLMQRLHHQ
                     GGDMATKGKVDVLADMSRHDQERLHQLVSNHARYTGSTRARHILDNWAEYRTKFVKVM
                     PLEYRRALKEMELQAAKAAAE"
BASE COUNT         2029 a         3360 c         3294 g         1867 t
ORIGIN      
        1 aagtctaggg aggaaacgcc caaaggaggg ctatcgaacg agggcaaacc gtcgtccgat
       61 gtggaaagag taatgttgcg ccgcacaaat ttcaagcctg aatcttggct tccgttaaaa
      121 aacctttccc gctcgcttcc tccgaaaggc cattcttggc cggatggcaa gatcatcacg
      181 cattcaggaa ttgcgcgaaa cgccccgaaa aagtgctaaa atataatcac gcctttataa
      241 tataagctga ttcaggcgca aaattataaa aaaggccacc cacgaagggt ggcccaagtc
      301 tagggaggaa acgcccaaag gagggcacgc aaggcagaaa gactcatgcc ttgcaccgac
      361 actaatatat gtgcgtcgca caaaaatcaa gttcatttgt aaactaaatg gtcactcttt
      421 ggcatagcca gttgcgtgtg gctgcaaacc cgctatgtgc ggtcctgacc ccaatccgct
      481 ttggccgttc gaggccatga aaggcttccc atgcgtccgg tcgatctcgt cgaatttctc
      541 gatcatcttg cgctgctttc gggtgaagcg atcctgccgt tcttccgaaa tcatatctcg
      601 gtgaccgaca aggccggaaa aggcgtgttt gatcccgtca cagaggccga tcgcgccgca
      661 gaactggtga tccggcgcga gatcaagcag cggtttcccc agcacggcat tctgggcgag
      721 gaattcggtg gcgatcatga ggaggccgag ttcgtctggg tggtggatcc gatcgacggc
      781 acgcgctcgt tcatctgcgg cctgccggtg tggggcacgc tgatcggact caagcgtttc
      841 gatgctgctg tgctcggcct gatgcaccag ccctacaccc gtgaacgctt cttcggcgat
      901 ggggcccgcg cctggctgaa agggccgtca ggcgaacgca agctgtcaac gcgcgccacc
      961 accgaccttg ccgaggcgac gctgatgacc accaccccgg cattgttcaa gggcagcatg
     1021 cgcgaggcct acgaccgggt tgaaaacacg gttcgcctcg cccggtatgg taccgattgc
     1081 tatgcctatg cgatgctggc gtccggtcac gtcgatcttg tcattgaaac cggtctcaag
     1141 gcctatgaca tcgttgccct gatcccgatc atcgagggtg caggcggcgt gatcacggat
     1201 ttttccggtg gctcggtcat aaacgggggt gatgtggtgg ttgccgccaa tcaccagctg
     1261 cacgaaaagg cgctgaagct gctcaaccgc tgaccgggct ggcgtccgaa gtctcggcgg
     1321 agagcccggg ctcttcacca ggctggcaat gcggcacaaa ggcatcgaac gctgcccaga
     1381 attcggcgcg gaacacatcc cgttccatca ggatttcgtg ttgcgcccct gtcagcgtca
     1441 ccgttgaggc gccgcgtgcg cgcagggcga aacgttccgc cgccggcgta tcgaccaccc
     1501 ggtcgccgcc ggccagcaca agcagcagcg ggcaggcaaa acgcagccca aattccggat
     1561 tgttgaagtc gccgatcagc ctgaacgccg cagcaaccca ggatatggtc ggcgcaccga
     1621 cggcaagctc gggggcggcg gtcaggatgg ccacagaccg ctcatgtcgc tccggatcgc
     1681 tggtcaggac attgtcctcg aatggggcaa aggcggtcgc cgtctgcggc gaggtgaccg
     1741 gcacatattg accgccaaga ccaaccacgt tgagcaggcg cgcggcgatg tgcccccagc
     1801 cgcctgtcgg ggcatgatag atccctagca tcggcgccgt aaccaccgca cgggccagcc
     1861 gctgctcgcc gtgattgagc gccagcgcca gaaccgcccc gcccatcgaa tgggcgaggc
     1921 caaagcacgg gcgcggaaga tcggatgccc cgatgaaggt gaggaccgcg tcgagatcgc
     1981 gcagatacaa aagaaaatca tcgacatggc ccttcatcgg gtccgacagt tcgcgcatcg
     2041 aaccgccctg tccgcgccag tcgaatgcaa ccacggcaaa tccgcgctgc atcagctcgc
     2101 cgatggtctc gaaataccgc tcgatgtatt cgctccgccc ctgcaggatc accacgctcg
     2161 ctcgcggatt ggcagggcga aacactgcgg cacgcagcgc caggccgtcg gtcgtggtca
     2221 gccggacgac ttcagccccg ggcggggccg gattggcgtc aattgaatac aggcgattga
     2281 gtgcgtcacg catcgcatcg tctccggctc tcgctgtgct tgtcatcgcc aagacaagaa
     2341 cacaagcctg tcgtcagggc ccggccagca cctcggccgc ccgttgtggc cccccgctgc
     2401 cgcgctcgat cacggtcttg agcccgagca cctcgccact ttgagcatgc accaccagcc
     2461 gcacgccctg gcggtcggag atgcgcgccc tggcaagata gaccgccccc cgccgccgca
     2521 catcatgcac atcgagatag cccgcctgca tcagccgccg ggtgacagcc tcgccgctga
     2581 gaccacgatt tgcgggaaga cttgcatggg aagggtcctt gagtgcggac agggccggca
     2641 gcttcgggct ccaactgccg acagcaagcc agatcgctgc cgccaatccg ccggcgatca
     2701 ccagcagcat ggcgcgcgac agccggcctg cggggcggcg gggccatgcg ataccgagag
     2761 tcggggaaag cttgctgagc atcgtgaggt cttaagcgcc ccagcctgaa ttgggctgga
     2821 agcggccatt aagccagcgt tcaactccgg ctcatccggg ggcgcgaccc ttgacattga
     2881 ctggaagcct tcccacctcc tgtgacgtcg caggccagca tggctgcgat ttcacaaggg
     2941 aacgccggcc attgtggcgg aaggttccat gaacactgtc gcttcaagga ggacagcatg
     3001 cgtcacttcg atttgactcc cctctatcgt tccaccattg gcttcgaccg gctgttttcg
     3061 ctgttcgatg acctgcccaa cctcgattcg tccggcggct atccccgcta caacatcgaa
     3121 aagaccggtg agcacgacta tcgcattacg ttggctgtcg ccggctttgg cgaggccgac
     3181 cttgccgtcg aggtggccga tggcgtgctg attgtgcgcg gcacccggca ggaaaaggac
     3241 ggcgaagcac gccccgagat gctgttccag ggcattgccg cgcgcgcgtt cgagcgtcgg
     3301 ttccagcttg ccgacagcgt gaccgtggcc ggtgccagat tggaaaacgg cctgctctcg
     3361 gtcgatctca agcgcgagat tcccgaggcc cgcaagccgc gcatgatccc gatcgggcac
     3421 ggcgcaaagc cggtggaaat cgcccattga ttggggtgtg ctcctgactc catgaatacc
     3481 ggcaggcgcc cgcaggggcg cctgtttgcc gttcagtcaa gcggcggcgg agggatggcg
     3541 gagccgtcgc ccccgcgtcc gctgcgaaaa tcgagcaacg agccgccttg cccctgcgat
     3601 ggcgccagtt gcggccccct gggatcgagc acggccggcg gcggagcatt gggaaggtcc
     3661 ggcagcaccg gcgtctgact ggtctgcaca ccgggagaga gctgcggcac agcctgcgtc
     3721 gagggggccg ttacgcccgg tgtcagagga atgacacgcg gcctttccct ggttcctgcc
     3781 gaggggtcgg tcttgccggg cgcggcaggg tcttttggaa gtgccagcgg tgaccgtttg
     3841 gcgctcgttg gtggcgcagg ggcctgttcc gcatcgttcg atctcggcac agcgtctcgc
     3901 tgcggcgccg gacgggtggc gcgcggcggc tcggcacgcg gcggtggaac gacgacgcgc
     3961 ggatcatcct cgggatcgat ctcgatgcgc gaaccgcgcg gaataccgcg ctggcggaat
     4021 gcttcgagga ttgatccatc atcgctcgac acgatcaacc gcaccggcac cccatacggg
     4081 tcgatggcat tgaccacata gacatcgcga ttgcgcagcg gccgcgtcag ccggctgaac
     4141 ccgcggtccc tcaggatttc gacgacctcc cggggtgaca gcacgtcatc gtcaaatccc
     4201 ctgccgaaat actgcgccgt cgcagggctc gcgaccggcg cagccagcgc cagaaccaga
     4261 gcgagtggca gcaggcgaac cagcggccga gcagggatcg ttcgacttct catcatgacc
     4321 aaacgcgagg ctaagccccg ctcctcatct gtcccgcccc tgtcccgttc tgggacagtg
     4381 cagccttgtt cagccgccct gaacggggtt tgaacggaca atgtcgatga aacgttcaac
     4441 ttcggccgaa cttgtggcga aggagcagac gaggcggacc acgtcttcgt cctccgcaag
     4501 ccggtcatcc ggcgcgagac tcttggctgt ccactcgtag aatgcagccc ccctggcccg
     4561 gagtgctgcg atcacctctc gcttgaggat cacgaagact tcattggctt cggtcggcca
     4621 ggcaaggcgc accccgggga ttgcggcaag ctccagtgac agacgcgtgg ccgcgccatt
     4681 ggcctttgcc gccaggtcca gccagtggcc ggcatcaaga taggcctcga actgcgccga
     4741 cagcagccgc cccttcgaga ccgtctgacc ggcgcgcttg cgacggaact tgaaatcctc
     4801 agccagcttc tcgttgaaga aaatgaccgc ctcggccatc agacaaccgt tcttggtgcc
     4861 gccgaacgac aggcaatcga ccccggcctt ccaggtcatt tcggccggtg tgcagccgag
     4921 cgtcagcagc gcgttggcaa accgcgcccc gtccatatgc aggctcatgc ccgcagcatg
     4981 ggccgtttca gcaatggccc ggatctcatc aagccggtgg accatgccgc attcggtcgc
     5041 ctgcgacagg ctgatgccgc gaaacggcgg atgattggtg ccgcccggtt cctccgcaag
     5101 gtgggcggca atcgccctgg cagagagctt ggcaccggtg ccggcgattc caaccagctt
     5161 ggcaccgccg gcgaagaact ccggcgcgcc gcattcgtcg tcgatgatgt ggctttcccg
     5221 atggcacagg gcggccgcat agggcggcac catcgccgcg atcgccagcg cattggctgc
     5281 cgtgccggtg gtcaccagaa aactcgtcac cttgcgctcg aacagggcat caaacgccgc
     5341 ctcgactcgc tgggtgatcg gatcagcacc ataggatgca agcgctccgc tgttggctac
     5401 cgccaacccc gccatcacct tgtcggaagc acccactaca ttgtcggaag tgaagatcat
     5461 gctgcggccc gctgcccaat gtccgttttt gtgatcggaa cctgattgtc attcatttgg
     5521 caaggggcag ctacaggccc ttgcaaatcg gcaacagcca ggaaaacacc gaatcggccc
     5581 ccttgtgcca gcgggcacga tctgcgtaag tgcgagtccc ggttagggca gagttgctga
     5641 cctattgcgt cgcagcccgc cggacccgca ggtgcgcggg aaaaagcgtg gacatagccc
     5701 aagctgccgg ggtggcagca aggagagacg acatgaccgt ccggttcgtg aacgatcgcg
     5761 cgcaatccca tgacagcggg gaggcgcaga gccgcaccct tcgggtcgtc gatcccggcc
     5821 ttcctgtggc agagggtctt tatgaccctg ccttggaaaa ggattcctgc ggcgtcggct
     5881 tcatcgccaa catgaagaac gtcaagagcc acgcgatcgt aacgcagggc ctgcagattc
     5941 tccacaacct cgatcatcgc ggcgcggtgg gcgctgatcc aaaggctggc gatggttgcg
     6001 gcatgctggt gcagatcccg catcgtttct tcaaggagga atgcgccaag ctcggctacg
     6061 atctgccgga gccgggccat tacgccatcc tccagttgtt cctgccgcgc gacaaggccg
     6121 gccgccggac cgtgcagaag atcgtcgaca aggtgatcaa ggccgagggc cagaggctga
     6181 ttggctggcg cgacgttccg gttgacaatg gcgacctcgg ggtcagcgtt ctgccgacag
     6241 agccggtgca tcgtcaggtg ttcatcggtc gcggtgaagg catcgccaat gaggatgagt
     6301 tcgagcgtcg cctgttcatt ctgcgcaagg tcatctccaa cgcagtctat gacctcaagg
     6361 acccgacgac aaagggctat tacccagtct cctcgtcctg ccgcacggtt gtctacaagg
     6421 gcatgctgct ggccggccag ctcggcagct attttgccga tctcaacgat ccgcgctttg
     6481 aaagcgcgct ggcgctggtg caccagcgtt tttcgaccaa caccttcccg acctggtcgc
     6541 tggcccatcc ctaccggatg gtggcgcata acggcgagat caacaccctg cgcggcaacg
     6601 tcaactggat ggccgcgcgg caggcgagtg tcgattccga gttgttcggc agtgacattt
     6661 ccaagctctg gccgatctcc tacgaaggcc agtcggacac ggcctgcttt gacaacgcgc
     6721 tcgaattcct cgtccagggc ggctattcgc tcgcccatgc catgatgatg ctggtgcccg
     6781 aagcctgggc cggcaatccg ctgatggatg atcagcgccg cgccttttac gaatacaatg
     6841 ccgccctgat ggagccatgg gacgggccgg ccgcggttgc cttcacggac ggacgccaga
     6901 tcggcgccac cctcgatcgc aacggcctgc gccccgcgcg ctattttgtc accgacgatg
     6961 gcctggtggt catggcttcc gagatgggcg tcctgccgat acctgaagag aaaatcgtca
     7021 ccaagtggcg cctgcagccg ggcaagatgc tgcttatcga ccttggcgaa ggacgcatca
     7081 tctccgatga agagatcaag gcagagctcg cctcagccaa tccctacaag gactggctga
     7141 agcgcaccca gatcgtgctt gaggacctgc cgccggtcga gccgcgcgcc ccgcgcacgg
     7201 acgtgccgct gctcgacaag caacaggcct ttggctactc gcaggaggat ctggcgctgc
     7261 tgatggcgcc gatggccacc accgggcagg aagcagtggg ctcgatgggc accgacacgc
     7321 cgatctcggc gctgtcggac aagtccaagc tgctctacac ctatttcaag cagaacttcg
     7381 cgcaggtcac caacccgccg atcgatccga tccgcgaaga actggtgatg agcctggtct
     7441 cgttcatcgg gccgcggccg aacctttttg atctcgaggg cctctcccgc cgcaagcggc
     7501 tcgaggtgcg ccagccgatc ctgaccaacg aggatctcga gaagatccgc ggtatcggcc
     7561 atttcgagga ccgcttcgac accaagacgc tcgacataac ctatccggcc gaacgcggcg
     7621 ccggcggcat gcaggacgcc attgaacggc tgtgcgaccg cgccgaggcg gcggtggccg
     7681 gcggctacaa catcattatc ctgtccgatc gtctggtcgg gccggagcgg gtgccgattc
     7741 cggcgcttct ggcgacggcg gctgttcacc accacctgat ccgcaagggt ctgcgcacgt
     7801 cggtcgggct cgtggtcgag accggggaag cgcgcgaggt gcatcacttc tgctgtctcg
     7861 ccggctatgg ggccgaggca atcaacccct atctcgcatt cgagaccatg gaggcgatgc
     7921 acgagaaggg cgaattcccg cccgaggtcg aggccaagga agtcgtcaag cgctacatca
     7981 agtccgtcgg caagggtatc ctcaaggtca tgtccaagat gggcatctcg acctatcaat
     8041 cctattgcgg ggcgcagatt ttcgacgcgg tcggcctcgg ccagagcttt gtcgacaagt
     8101 atttcttcgg caccgccagc cgcatcggcg gcgtcggact tgccgaggtc gcgatcgaga
     8161 cggtgatgcg tcatcgcgat gctttcggtg acaatccggt gctcaagacc agcctcgagg
     8221 tcggcggcga atatgccttc cgcttccgtg gcgaggcgca tgcctggtcg ccgaccactg
     8281 tggccgcgct ccagcacgcc gtgcgtggca attccaagga gcgctacgaa gaattcgccc
     8341 gcgccatcaa cgagcagaac gagcggttgc tgaccattcg tggcctgttc cgcatcaagg
     8401 cggcagcgga agatggccgc aagccggtcg atgtgtccga agtcgagccg gcgaacgtga
     8461 ttgtccggcg ctttgccacc ggcgcgatgt cgttcgggtc catctcgcgc gaggcccaca
     8521 ccacgctggc gcgggcgatg aatgcgctgg gcggcaagtc aaacaccggc gagggcggcg
     8581 aggaatccga tcgcttccgg ccgctgccgg acgggcgttc caatccggaa cggtcggcga
     8641 tcaagcaggt cgcctcgggc cgtttcggcg tgacgacgga atatctcgtc aattccgaca
     8701 tgatgcagat caagatggcg cagggtgcca agcccggcga aggcggtcag ctgcccggcc
     8761 acaaggtcga tgcggtgatc gccaaggtgc gccattcgac cccgggcgtc ggcctcatct
     8821 cgccgccgcc gcatcatgac atctattcga tcgaggacct tgcccagctc atctacgatc
     8881 tgaagaacgt aaacccggcg ggcgatgttt cggtcaagct ggtctcggag gtcggagtcg
     8941 gcacggtcgc ggccggcgtt gccaaggcac gcgccgatca tgtgaccatt tccggcttcg
     9001 agggcggcac gggcgcatcc ccgctcacct cgatcaagca tgccggctcg ccgtgggaga
     9061 ttggccttgc cgagacgcac cagactctcg tcaggaaccg tctgcgttca cgcatcgcgg
     9121 tgcaggtcga cggtggcctt cgcaccggac gcgatgtcgt tgtcggagcc ttgctcggcg
     9181 ctgacgaatt cggttttgcc accgccccgc tgatcgcagc cggctgcatc atgatgcgca
     9241 agtgtcacct caacacctgc ccggtcggtg tcgccaccca ggatccggtg ctcaggaagc
     9301 gcttcatcgg cacgccggag catgtcatca actacttctt ctttgttgcc gaggaagtgc
     9361 gccagttgat ggcggagatg ggctaccgca cgttcaacga gatggtcggc cagatgcaga
     9421 tgctcgatcg gtccgaggct attgccgcat ggaaggccaa gggcatcgac ttcacacgcc
     9481 tgttccacaa acccgaggaa ggcgccgaaa ccggcatctt cgtcagcgag cggcaggacc
     9541 atcacctcga gaaggtgctc gaccggcagt tcatcgcggc ggcgtcaccc gccctgactg
     9601 acaagaccag ggttcgcgcc gagttcaaaa tcaccaacgt cgaccgcacc gcaggcgcga
     9661 tgctgtcggg cgaggtcgcc aaggcctatg gccatgcagg actgccggaa gatacgatcc
     9721 acctcaagct caatggcacc gccgggcaga gcttcggcgc ctgggttgcg cgcggcgtga
     9781 cactggaact cgaaggcgag gccaacgatt atgtcggcaa gggcctgtcg ggtggcaaat
     9841 tgatcatcta tccctcggca aaggcgcagc agatcaagcc ggaagcctcg atcatcgtcg
     9901 gcaacaccgt gctttacggc gcgatctccg gcgaatgcta tttccggggt gtcgccggcg
     9961 aacgcttcgc cgtgcgcaac tccggtgcca tcgctgtggt ggaaggcacc ggcgaccatg
    10021 gctgcgaata catgaccggc ggcatcgtcg tcgtgctcgg caacaccgga cgcaacttcg
    10081 ccgccggcat gtcgggcggc atcgcctatg tgctggacga ggacaagacc ttcgccaagc
    10141 gctgcaacat gtcgatggtc gaccttgagc cggtcccgga agaggaagac ctgatgcagc
    10201 gcctgcatca ccagggcggt gacatggcca ccaagggcaa ggtcgatgtt ctggccgaca
    10261 tgtcgcgtca tgatcaggaa cggctgcatc agcttgtctc caatcatgcg cgctacaccg
    10321 gttcgacccg tgcacggcac attctcgaca actgggccga gtatcgcacc aaattcgtca
    10381 aggtgatgcc gctcgaatat cggcgcgcgc tgaaggaaat ggaattgcag gcggccaagg
    10441 ccgcggcgga atagccatcc cgattgctcc ggtggcgctg ccaggcgccg ccggaacgct
    10501 agatcacgcc gcattttgat ggaatcatca aaatgcggat aacgtgatcg
//
LOCUS       DAOMXG010000079        10430 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_3410_length_10430_cov_8.869108, whole genome shotgun sequence.
ACCESSION   DAOMXG010000079 DAOMXG010000000
VERSION     DAOMXG010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 10430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10430
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3410_length_10430_cov_8.869108"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..567)
                     /locus_tag="PLQ11_06845"
     CDS             complement(85..567)
                     /locus_tag="PLQ11_06845"
                     /inference="COORDINATES: protein motif:HMM:NF017913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HRK24654.1"
                     /translation="MSQQDTRKFPDGTDAPMFIRRTNRFALKLMSWVNAFAHLVLGLA
                     LAGAVLLFTWLFVKDVANGLSHDNMAEGFLHGLGTIMLLWTISALITAEIRYLRGHPL
                     QIDTFVEVAIVVILRKLITMPVQSALPAPAEFILWVASAVLLAIMFVIVRFAISMGRD
                     "
     gene            complement(689..1255)
                     /gene="rplI"
                     /locus_tag="PLQ11_06850"
     CDS             complement(689..1255)
                     /gene="rplI"
                     /locus_tag="PLQ11_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HRK24655.1"
                     /translation="MEVILLERVAKLGQMGEIVKVRPGYARNFLLPQKKALRATEDNK
                     KVFETRRAQLEARNLELKKEADAVGKKLNGQSFVLIRSAGESGMLYGSVSTRDLAEVM
                     TANGFTVSRNQVVLNAPIKLLGLHTVPVHLHPEVDVAVTINIARSPEEAERQARGENV
                     TAREAHNIDDLGLEIGAALAEAGPTAEL"
     gene            complement(1288..2244)
                     /locus_tag="PLQ11_06855"
     CDS             complement(1288..2244)
                     /locus_tag="PLQ11_06855"
                     /inference="COORDINATES: protein motif:HMM:NF021506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HRK24656.1"
                     /translation="MAQAILIGLIAGLASALLSGLLTPGSLLASLLFVIAPLPLMIVG
                     LGWHPLVAALGALAGCLVMDLGLSNTAALYFGLLVGLPSYLFSELLLRLLAFLPALPP
                     ERAGRLGGTILLGSIGAYAVLVTLGGAMVIDTSHAGFVQRVARSVETLFRDIASGGNK
                     LPLPGGAQDLAALARLYAQMLPPMLAFIIAVMLSLSLWLGLKVARTSQRIAYPVLPAH
                     ALSLPSAALFVFIGGFGLAQLGGYIGLFGTLVMVAVTFALILNGLAVLHARTLGSPSR
                     ALILSGVWGAVLVFGIPAFALALVGAVDCAFGLRNTRNKPLV"
     gene            complement(2361..2627)
                     /gene="rpsR"
                     /locus_tag="PLQ11_06860"
     CDS             complement(2361..2627)
                     /gene="rpsR"
                     /locus_tag="PLQ11_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HRK24657.1"
                     /translation="MSFADRKEGGAAAGGRRPFFRRRKSCPFQGEGAPKIDFKDVKLL
                     SRYISERGKIVPSRITAVSAKKQRELAQAIKRARFLGLLPYVIK"
     gene            complement(2627..3046)
                     /gene="rpsF"
                     /locus_tag="PLQ11_06865"
     CDS             complement(2627..3046)
                     /gene="rpsF"
                     /locus_tag="PLQ11_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HRK24658.1"
                     /translation="MPLYEHVFLARQDISAQQVEGLTDQYKAVIEAAGGAVTKVEYWG
                     VKSLAYRMNKNRKAHYALMNISASAPAVAEMERQMRINENILRFMTVRVEQLEDGPSA
                     MLQKRERDEKRDAERGSYGDRPPRRRDREEDAGGEDE"
     gene            3327..4160
                     /locus_tag="PLQ11_06870"
     CDS             3327..4160
                     /locus_tag="PLQ11_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRK24659.1"
                     /translation="MDQIIVTAHGAAIPAIGYGTWTLRGEVARDRVRHALACGYRHID
                     TAIMYENEIEVGEGMRQSGVPRDEIFLTTKVPPALIGEADLVDAAAGSIRRLGVDQVD
                     LLLIHWPNPEIDFTGSIRALCKARTLGLARHIGVSNFTIAHLDQAWQVTSEPLVTNQC
                     EYHPRLDQSRLIAACRRHGMSFTSYSPVARGALFGSPPLLSAARAHGKSESQVLLRWH
                     IQQPGIIAVPRSATPARIAENLDVFDFSLTDAEMAAISALKAADGRIVRPAWSPQWDE
                     S"
     gene            4167..4961
                     /locus_tag="PLQ11_06875"
     CDS             4167..4961
                     /locus_tag="PLQ11_06875"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HRK24660.1"
                     /translation="MYWVSKIIWLAAEPGNLLMLTLIAGIMLSARERFARLGNGLIIA
                     ATALLLLLGLSPIGTLMLRPLEARFPVYGETGAKVDGILVLGGVLATDVTLARGQPAL
                     NEAAERLTILLPLTRQFPEARIVFSGGAGEFTGAPISESEAVQTFVAEMMPGRAVIYE
                     RNSRNTFENARLSFDQIRPKPEERWLLVTSAWHMPRAYGLLRKVGWPVVAYPVDFRTS
                     GTSDDYRVFSGVSQGLRRVDMAAKEWIGLVFARLAGQSDSLLPGPE"
     gene            complement(5049..5324)
                     /locus_tag="PLQ11_06880"
     CDS             complement(5049..5324)
                     /locus_tag="PLQ11_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3253 domain-containing protein"
                     /protein_id="HRK24661.1"
                     /translation="MIADSDTLARLEAEMLRLVAERGPDRTACPSEAARAIGGPHPDQ
                     WGPLMTPARRIAVRLAGEGRIVITRKGRPVDPNDFRGVYRLGLPRSD"
     gene            5542..6543
                     /locus_tag="PLQ11_06885"
     CDS             5542..6543
                     /locus_tag="PLQ11_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016920890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HRK24662.1"
                     /translation="MALPEISMRQLLEAGAHFGHQSHRWNPKMAQYIYGVRNKIHIID
                     LSQSAPMLQRALQAISDTVARGGRVLFVGTKRQAQDSIAESAKRCAQYYVNSRWLGGM
                     LTNWKTISASIQRLRKVEETLAAGAHGLTKKERLMLSREQEKLDRALGGIKDMGGTPD
                     LIFVIDTNKEQLALQEARRLNIPVVAIVDTNCDPDGIAFPVPGNDDAGRAIALYCDLV
                     SRAVIDGIGRGAGDMGVDLGAAEAPVVEDLPAAGASESDFVFERLSAPRGAPDDLTKL
                     GGLGPELEKKLNDAGIFHFWQFSAMAPADETKLDADLKLHGRIGREGWIEKARGLLA"
     gene            6647..7570
                     /gene="tsf"
                     /locus_tag="PLQ11_06890"
     CDS             6647..7570
                     /gene="tsf"
                     /locus_tag="PLQ11_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HRK24663.1"
                     /translation="MAEITAALVKELREKTGAGMMDCKGALAETGGDLEAAIDWLRTK
                     GLAKAAKKSGRLAAEGLVAAAVSGKAGVVVEVNSETDFVARNDQFQGLVRSIAGIALK
                     GAGEIEALKAAAYPGGGTVSEAIANTVATVGENMSLRRVAGVSVSDGVVASYIHNQVV
                     EGLGKIAVLVALESKGDAAALAALGRQVAMHVAATNPQALDAASMDPAVVAREKAVLT
                     DKAAASGKPANVIEKIVESGIKTFYKEVCLVDQPFIHDGAKTVLQAVNEAAKSAGAPI
                     ALKGYVRFALGEGIEKEVSDFAEEVKKAGGA"
     gene            7652..8374
                     /gene="pyrH"
                     /locus_tag="PLQ11_06895"
     CDS             7652..8374
                     /gene="pyrH"
                     /locus_tag="PLQ11_06895"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HRK24664.1"
                     /translation="MTKPKPLYKRALVKLSGEALMGSESFGIHQPTVRQICADLVEAA
                     NLGVELAVVIGGGNIFRGAQMSGMGLSRPAADSMGMLGTVMNAIALEAQVEALGHSAR
                     AMSGVAMPSVVETYTRQQASDHLSKGRIVILAGGTGNPFFTTDTGGALRAAELGCDVM
                     LKATQVDGIYTADPKKDRTATRYDRVSYDEVIAKDLKVMDTAAFALARDASLPIIVFS
                     IHQPGSIAKVLTGTGHGTTVGR"
     gene            8452..9012
                     /gene="frr"
                     /locus_tag="PLQ11_06900"
     CDS             8452..9012
                     /gene="frr"
                     /locus_tag="PLQ11_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HRK24665.1"
                     /translation="MADTFDLNDLKRRMQGAVASLKHDLASLRTGRASANLLDPIEVE
                     AYGARMPIAQVATVSVPEPRLLSVQVWDRGMVHAVEKAIRDSSLGLNPNTEGQVIRLR
                     IPELNQQRRQEMVKVAHKYAEAARVAARHVRRDGMELLKKLEKDGKMAKDDATRAHDQ
                     VQKATDAAIAEIDAALVHKEKEIMQV"
     gene            9033..9791
                     /locus_tag="PLQ11_06905"
     CDS             9033..9791
                     /locus_tag="PLQ11_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HRK24666.1"
                     /translation="MENAARAQFDPVPAKGGGAAPFGLKHVGIIMDGNGRWAKQRGLP
                     RAFGHRAGVETLRGVVRTFSDLNIPYLTVYSFSSENWARPASEVAELMSLLRLFIRSD
                     LADLNARNVRIKVIGERAGLQPDIVALLNEAEDKTRTNTGLTFAVAFNYGARHELVMA
                     ARRLAELARTGRLEPCDISEARISAELTTAGMPDPDLIIRTSGEQRLSNFLMWQAAYA
                     EFVFTPVLWPDFDRAALETCLTEFGARTRRFGGL"
     gene            9791..>10430
                     /locus_tag="PLQ11_06910"
     CDS             9791..>10430
                     /locus_tag="PLQ11_06910"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRK24667.1"
                     /translation="MAEAEPAGRSELAQRVISAVIMASAAILLAWWGGWPFAIVWGLI
                     GGVALWEWSRLIGGMASPGWLAVGAVYAGVIMLGTLAVRGSGALGFIAILFMFAVVWL
                     TDIGAYFAGRTFGGPKFAPRISPKKTWSGVLGGLAAGILGGCAMLAVAGVPLRPAHGL
                     LALGLGMLVVAGDLFESFLKRRFGVKDAGALIPGHGGVLDRLDGYTFAIGAAA"
BASE COUNT         1869 a         3321 c         3478 g         1762 t
ORIGIN      
        1 ccggtagctc agcggtagag cacgtgactt ttaatcatgt ggtcgagggt tcgattccct
       61 cccggctcac caccccccgt ggtttcaatc cctgcccatg ctgatggcga agcgcacgat
      121 cacgaacatg atcgcgagca gcacggcaga ggcgacccac agaatgaact cggcaggtgc
      181 cggcagcgcc gactgcaccg gcatggtgat cagcttgcgc aggatcacca caatcgccac
      241 ctcgacgaat gtgtcgatct gcagagggtg gccgcgcaga tagcggatct cggcggtgat
      301 cagcgccgag atggtccaca acagcatgat cgtgccaagt ccgtgcagaa acccctcggc
      361 catgttgtca tggctgaggc cattggcgac gtctttcaca aacagccagg tgaacagcag
      421 caccgccccg gcaagcgcca gccccagcac cagatgcgcg aacgcattga cccaggacat
      481 cagcttgagc gcaaatcggt tggttcgccg gatgaacatc ggcgcgtcgg tgccgtcggg
      541 aaacttcctg gtgtcctgct ggctcatgag cgccccctga ccagggcagg atgggcgcgc
      601 gcgcgccgga tgccaagcac gcatattgcc gccagacaaa cgaaaagggc ggccgtagcc
      661 gccctgtccc tgccaatatg gcaacaattc acagttcggc ggtcggtccc gcctcggcta
      721 gcgccgcgcc gatctcgagg ccgagatcgt cgatattgtg cgcctcgcgg gcggtcacgt
      781 tctcgccgcg ggcctgacgc tccgcctctt ccggcgagcg ggcgatgttg atggtcaccg
      841 cgacatcgac ctccggatgc agatgcaccg gaaccgtgtg caggccaagc agcttgatcg
      901 gggcgttgag caccacctga ttgcgcgaga cggtgaagcc gttggcggtc atgacctcgg
      961 caagatcgcg cgtcgacacc gagccataga gcatgcccga ctcgcccgcc gagcggatca
     1021 gcacgaagct ctggccgttg agcttcttgc caacggcatc ggcttccttc ttcagttcga
     1081 ggttgcgggc ctcaagctgg gcacgccggg tctcgaacac cttcttgttg tcctcggtgg
     1141 cgcgcagggc cttcttctgc ggcagcagga aattgcgggc atagccgggg cgaaccttga
     1201 cgatttcgcc catctggcca agcttggcga cacgttcgag caggatgact tccatttctg
     1261 tttctccgtt gatgggttgg atgggggtca gaccagaggt ttgttccggg tgttcctcag
     1321 gccaaaagca caatcgaccg cgccgaccag cgccagcgcg aaggcgggaa tgccgaaaac
     1381 gagaaccgca ccccagacgc cggaaagaat cagggcgcga cttggcgagc caagcgtgcg
     1441 ggcatgcagc accgccagcc cgttcaggat cagcgcaaag gtcaccgcca ccatgaccag
     1501 cgtgccgaaa agcccgatat agccgccaag ctgggccagt ccgaacccgc cgatgaacac
     1561 aaacagcgcc gccgacggca gcgacagcgc atgcgccggc aataccggat aggcgatccg
     1621 ctgcgaagtt ctggccactt tcagccccag ccacaggctc aggctcagca tcaccgcgat
     1681 gatgaaggcc agcatcggcg gcagcatctg ggcatagagc cgggcaagcg ccgccagatc
     1741 ctgcgcaccg cccggcagcg gcagcttgtt gccgcccgag gcgatgtcgc ggaacagcgt
     1801 ctcgacgctg cgcgccacgc gctgcacgaa accggcatgc gacgtgtcga tcaccatcgc
     1861 gccgcccagc gtcaccagaa ccgcataggc gccgatcgat cccagcagga tcgttccgcc
     1921 aaggcgcccg gcgcgttccg gcggaagcgc cggcagaaag gccagcagac gcagcagcag
     1981 ttcggaaaac agataggacg gcagaccgac cagcagcccg aaatagagcg cggcggtatt
     2041 gctcagcccg aggtccatca ccaggcagcc ggcaagcgcg ccaagggccg cgaccagcgg
     2101 atgccagcca agtccgacga tcatcagcgg caggggcgcg atcacgaaca gcaggctggc
     2161 aagcagcgag ccgggcgtca gcagccccga caacagcgcc gaggccagcc ccgcgatcaa
     2221 tccgatgaga atggcctgag ccatgattgt ccgctgtccc ttacggttag gggtcttgcc
     2281 ccagcgatac ctgatccggg acagtcccgc ccaggcgaca gatggatgcc gggcgccggc
     2341 tgagcggcgc ccggaaggtc ttacttgatc acatacggca gcaggccgag gaagcgggcg
     2401 cgcttgatcg cctgggcgag ttcgcgctgc ttcttggccg agacggcggt gatgcgggac
     2461 ggaacgatct tgccgcgctc ggaaatgtag cgcgagagca gcttgacgtc cttgaaatcg
     2521 atcttcggcg cgccctcgcc ctggaacggg caggacttgc ggcgacggaa gaacgggcga
     2581 cggcctccgg cggcggcgcc gccttccttg cgatcagcaa acgacattat tcgtcctccc
     2641 cgccagcatc ttcttcacgg tcacggcggc gcggcggacg atcaccatag ctgccacgct
     2701 cggcgtcacg cttctcgtcg cgctcgcgct tctgcagcat cgccgacggt ccgtcctcga
     2761 gctgttcgac acgcacggtc atgaagcgca ggatattctc gttgatgcgc atctgccgct
     2821 ccatctcggc gacagccggg gccgaggcgg agatgttcat cagcgcataa tgcgccttgc
     2881 ggttcttgtt catgcggtag gcgagggact tgacgcccca gtattccacc ttggtcaccg
     2941 cgccgccggc cgcctcgatc accgccttgt actggtcggt gaggccctca acctgctgcg
     3001 cggaaatgtc ctggcgtgcc aggaacacat gctcgtaaag cggcatgtcc gtgcctttct
     3061 tgcatcgctc ggcgccggaa ttggccccga gatcatccaa cggcgctgag cccttcgagc
     3121 ccggaaaggc ccgacagaac aacgaaggcg tcgacaaggg agaccggatc gggaagatcc
     3181 tgcgggcaac agcccgccct cctttcagcc gccgaccgaa ggatcgcgcg gctataggag
     3241 cattcggccc ggaaggcaag gcccaacatg ctggacagca gggcaggaat ccggatgatc
     3301 gcctccgtat gtcagggaga caggctttgg accagatcat cgtcacggcg catggggcgg
     3361 ccattccggc catcggctac ggcacctgga ctctcagagg cgaggtggcg cgcgatcgcg
     3421 tccgccacgc gctcgcctgt ggctaccgcc acatcgacac cgcgatcatg tatgaaaacg
     3481 agatcgaggt gggcgagggg atgcgccagt ccggcgtgcc gcgcgacgag attttcctga
     3541 ccaccaaggt tccgccggcg ctgatcggcg aggctgatct ggtcgatgcg gcggccggca
     3601 gcatccgccg cctcggggtc gatcaggtcg atctgctgtt gatccactgg cccaaccccg
     3661 agatcgattt caccggctcg atccgcgccc tgtgcaaggc aaggacgctg gggctggcac
     3721 gccatatcgg cgtttccaac tttaccatcg cccatcttga tcaggcctgg caggtgacga
     3781 gcgaaccgct ggtcaccaac cagtgcgaat accatcccag gctcgaccag agccggctca
     3841 tcgccgcctg ccggcgccat ggcatgtcgt tcacctccta ttcgccggtg gctcgcggcg
     3901 cgctgttcgg atcgccgccg ctgctgtcgg cagcgcgcgc ccatggcaag agcgaaagcc
     3961 aggtcctgct gcgctggcat atccagcagc cgggcatcat cgccgttccc cgctcggcaa
     4021 cgccggcccg cattgccgag aatctcgacg tattcgattt ttcgctgact gatgccgaaa
     4081 tggcggccat ttccgccctg aaggcggcgg atgggcgcat cgtcaggccg gcatggtcgc
     4141 cgcaatggga cgaaagttaa ggcgtcatgt attgggtttc gaagattatc tggctggcgg
     4201 ccgagcccgg caatctgctg atgctgacgt tgatcgccgg gatcatgctt tcggcacgcg
     4261 agcggttcgc ccggcttggc aatgggctga tcatcgcggc gaccgcgctg ctgctgctgc
     4321 tcggactgtc gccgatcggc acgctcatgc tgcgcccgct ggaggcccgc ttcccggtct
     4381 atggcgagac aggcgccaag gttgacggca ttcttgtgct cggcggggtt ctggccaccg
     4441 atgtgaccct ggcacgcggc cagccggcac tcaatgaagc cgcagaacgg ctgacgatcc
     4501 tcctgccgct gacccggcag tttccggaag cgaggatcgt tttctccggc ggggcggggg
     4561 agttcaccgg agcgccgatt tccgaatcgg aggccgtgca gacatttgtt gccgagatga
     4621 tgcccggccg cgcggtgatc tacgaacgca actcgcgcaa cacgttcgag aatgcccgcc
     4681 tgtcgttcga tcagatccgg ccgaagccgg aggaacgctg gctgctggtc acttcggcct
     4741 ggcacatgcc gcgtgcctat ggcctgctcc gcaaggtcgg ctggccggtc gtggcctatc
     4801 ccgtcgactt tcgcaccagc ggcaccagcg atgattaccg ggtcttttcg ggagtctcgc
     4861 aggggctgcg gcgggtcgac atggctgcca aggaatggat cgggctggtc tttgcccggc
     4921 tggcggggca gagcgacagc ctgttgccgg ggccggaata gagcatgctg cgattaagtg
     4981 gaaggcggtc aatcgccaga acagccatca aatccgcgga tataacgcgg atataatagg
     5041 tcctgaccct agtccgatcg cggcaggccc agccggtaga cgccgcggaa atcgttggga
     5101 tcgacagggc gccccttgcg ggtgatgacg atgcgtcctt ccccggcaag ccgcaccgcg
     5161 atgcgacggg ccggcgtcat caaggggccc cactggtcgg gatgcggacc gccgatcgca
     5221 cgcgccgctt ccgaggggca ggcggtgcgg tccggcccgc gttcggcgac aaggcgcagc
     5281 atttcggctt ccagccgggc gagggtatcg gaatcagcaa tcatggtggc agccttctat
     5341 ccgggaaggc atctccaggc taccggcgat gcagccgtgc gctgccgccg tcgactgacg
     5401 gcaaaaaggg ccgtgccccc ttgcttttgt ctgcgaattg ggcttaaggg ggcgccatca
     5461 cacacgcgag ggcaggtttc gaccgtctgg tgccggatcc gtccggtatt tcctcgcgga
     5521 ggcttaaacc ggagaagacc aatggcgttg cccgagattt cgatgcggca gctgctggag
     5581 gctggtgcgc atttcggcca ccagtcccac cgctggaacc cgaagatggc gcagtatatt
     5641 tacggcgtcc gcaacaagat tcacatcatt gacctcagcc agtcggcgcc gatgctgcag
     5701 cgcgcgctgc aggcgatctc cgacaccgtg gcgcgcggcg ggcgcgtcct gttcgtcggc
     5761 accaagcgcc aggcgcagga ttcgattgcc gaaagcgcca agcgttgcgc ccagtattac
     5821 gtcaattcgc gctggctcgg cggcatgctg accaactgga agacgatctc tgcctcgatc
     5881 cagcggctgc gcaaggtcga ggagacgctg gccgccggcg cgcacggcct gaccaagaag
     5941 gaacgcctga tgctgtcgcg cgagcaggaa aagctcgacc gcgcactcgg cggcatcaag
     6001 gacatgggcg gcacgcccga cctgatcttc gttatcgaca ccaacaagga gcagctcgcg
     6061 ttgcaggagg cgcgccgcct caatatcccg gtggtggcga ttgtcgatac caattgcgat
     6121 cccgatggca tcgccttccc ggttccgggc aatgacgatg ccggccgggc gatcgcgctg
     6181 tattgcgatc tggtgtcgcg tgcggtgatc gacgggatcg gtcgcggcgc tggcgacatg
     6241 ggcgttgacc tcggcgctgc cgaggctccg gtggtcgagg acctgccggc ggcaggtgca
     6301 agcgagagcg atttcgtgtt cgaacggctg tctgcgccgc gcggcgcgcc ggacgatctg
     6361 accaagctcg gcggcctcgg cccggagctt gagaagaagc tgaacgatgc cggcatcttc
     6421 cacttctggc agttttccgc catggcgccg gccgacgaga ccaagctcga cgccgacctc
     6481 aagctgcacg gccgcatcgg ccgcgaaggc tggatcgaga aggcgcgcgg gcttctcgcc
     6541 tgagcgaccc cgaacacacg ctgacacgtg cgggcccggg gcgagagctg ccgggccttt
     6601 gcgtgaagaa atcacgataa acgacggatg aagaaggata aatctcatgg ctgagattac
     6661 cgctgcgctg gtcaaggaac tgcgcgagaa aaccggcgcc ggcatgatgg actgcaaggg
     6721 cgcgctggcc gagaccggcg gcgatctgga agccgccatc gactggctgc gcaccaaggg
     6781 cctggccaag gcggccaaga agtccggccg tctggcggcc gaggggctgg tcgctgcggc
     6841 ggtgtccggc aaggccggcg tggtcgtcga ggtcaattcc gagaccgatt tcgtcgcccg
     6901 caacgaccag ttccaggggc tggtgcgttc gatcgccggg atcgcgctca agggcgccgg
     6961 cgagatcgag gcgctcaagg ctgcggccta tccgggcggc ggcacggtga gcgaggcgat
     7021 tgccaacacg gtcgccacgg tgggcgagaa catgtcgctg cgccgcgtcg ccggtgtttc
     7081 ggtgagcgat ggcgtcgtgg cttcctacat ccacaatcag gtggtcgaag gactcggcaa
     7141 gatcgcggtg ctggtggcgc tggaatcgaa gggcgacgcc gccgcgctag cggcgcttgg
     7201 ccggcaggtg gcgatgcatg tcgcggccac caatccgcag gcgcttgacg cggccagcat
     7261 ggacccggcg gtggttgcgc gcgaaaaggc ggtgctgacc gacaaggcgg cggcctcggg
     7321 caagccggcc aacgtgatcg agaagattgt cgagagcggc atcaagacct tctacaagga
     7381 agtctgcctc gttgatcagc cgttcatcca cgacggcgca aagacggtgc tgcaggctgt
     7441 gaacgaagct gccaagtcgg cgggcgctcc gatcgcgctc aagggctacg tgcggtttgc
     7501 actcggcgag ggaatcgaga aagaagtctc ggacttcgcc gaagaggtga agaaggctgg
     7561 cggcgcctga caagcaatcc tacccgtatg ggcagggcaa gggcggcggc aagccgccct
     7621 ttttgtttgc ctgtcacgcc ggaggtgccc cttgaccaaa ccgaaaccgc tctacaagcg
     7681 cgccctcgtc aagctgtccg gcgaggcgct gatgggttcc gaatccttcg gcatccacca
     7741 gccgacggtg cggcagatct gtgccgatct ggtcgaggcc gccaatctcg gggtcgaact
     7801 ggcggtggtc atcggcggcg gcaacatctt ccgcggcgcc cagatgtccg gcatgggcct
     7861 gtcgcgcccg gccgccgatt cgatgggcat gctggggacg gtgatgaacg cgattgcgct
     7921 ggaggcgcag gtggaagcgc tcggccattc ggcccgcgcc atgtccggcg tcgccatgcc
     7981 ctcggtggtc gagacctaca cccggcagca ggcttccgat catcttagca agggccgaat
     8041 cgtcattctg gccggtggca ccggcaatcc attcttcacc accgacacgg gcggggcgtt
     8101 gcgtgcggcc gaactcggct gcgacgtgat gctcaaggcc acccaggttg acggcatcta
     8161 cacggccgat ccgaagaaag acaggacggc aacccgatac gaccgggtca gctacgacga
     8221 ggttatcgcc aaggatctca aggtgatgga cacggcggct ttcgcgctgg cgcgcgatgc
     8281 cagcctgccg atcatcgtct tttccatcca ccagccgggc agcatcgcca aagtgctgac
     8341 aggaaccggg cacggcacca ccgttgggcg gtgaattgac cttcgtcaac ccttgcggca
     8401 agccgccggg cggcgtaacc agaacggacc acagatcccg gaacacctgt catggccgat
     8461 acgttcgatc tcaatgacct caagcgtcgc atgcaggggg cggttgcctc cctcaagcac
     8521 gatctggcct cgctgcgcac ggggcgtgcc tcggccaatc tgctcgatcc gatcgaggtg
     8581 gaggcttacg gcgcgcgcat gccgatcgcc caggttgcca ccgtctcggt gccggagccg
     8641 cggctgctca gcgtgcaggt gtgggatcgc ggcatggtcc atgcggtgga aaaggcgatc
     8701 cgcgattcct cgctgggcct caatcccaac accgaagggc aggtgatccg cctgcgcatt
     8761 ccggaactga accagcagcg ccgccaggaa atggtcaagg ttgcccacaa atatgccgag
     8821 gcggcccgcg ttgccgcccg ccatgtgcgg cgcgacggca tggagctgct gaagaagctg
     8881 gaaaaggacg gcaagatggc caaggatgac gcgacccgtg cccatgatca ggtgcagaag
     8941 gcgactgatg cggccattgc cgagattgat gcggccctgg tccacaaaga aaaagaaatc
     9001 atgcaggttt gaggacgaaa gccggacgat tgatggaaaa cgccgcgcgt gcccagtttg
     9061 atcccgttcc cgccaaaggc gggggcgcgg ccccgttcgg cctcaagcat gttggcatca
     9121 tcatggatgg caacggtcgc tgggccaagc agcgcgggtt gccgcgcgca ttcgggcata
     9181 gggctggagt ggagacgctg cgcggtgtcg tccggacatt cagcgatctg aatattccgt
     9241 atctgacggt ctacagcttc tcctccgaga actgggcgcg gccggcctcg gaagtggccg
     9301 aactgatgtc gctgctgcgg ctgttcatcc ggtccgatct ggccgatctg aatgcccgca
     9361 atgtccgcat caaggtgatc ggcgagcggg cagggctgca gccggatatc gtcgccctgc
     9421 tcaacgaggc ggaggacaag actcggacca acaccggtct gaccttcgcc gtcgccttca
     9481 actatggcgc gcggcatgaa ctggtgatgg cggccagacg gctggcggag ctggcccgca
     9541 ccggtcgcct tgagccctgc gacatcagcg aggcgcgcat ttccgccgaa ctgaccaccg
     9601 ccggcatgcc cgatcccgac ctgatcatcc gcacctcggg agagcagcgg ctgtcgaact
     9661 tcctgatgtg gcaggcagcc tacgccgaat tcgtgttcac gccggtgctt tggcctgatt
     9721 tcgaccgggc ggcgctggaa acctgcctga cggaattcgg cgcgcgcacg cggcggttcg
     9781 gcggtttgtg atggccgaag cggaacccgc cggccgcagc gagcttgccc agcgcgtcat
     9841 ttccgccgtg atcatggctt ctgccgccat cctgctggca tggtggggcg gctggccttt
     9901 tgccatcgtc tggggattga tcggcggcgt tgcgctgtgg gaatggtcgc ggctcatcgg
     9961 cggcatggct tcgcccggtt ggcttgcggt cggcgccgtc tatgccggcg tcatcatgct
    10021 ggggacgctg gcggtgcgcg gcagcggggc gcttgggttc atcgccatcc tgttcatgtt
    10081 cgcggtcgtt tggctgaccg atatcggtgc ctattttgcc gggcggacct ttggcgggcc
    10141 caaatttgcg ccgcgcatca gccccaagaa aacctggtcc ggggtgcttg gcggcctggc
    10201 tgccggcatt ctcggcggct gtgcgatgct ggcggtggcc ggcgtgccgc tgcggccggc
    10261 gcatgggctg ctggcgctcg gtctcggcat gctggtggtc gccggcgacc tgttcgaatc
    10321 gtttctcaag cggcggttcg gggtcaagga tgccggcgcg ctgattccgg ggcacggcgg
    10381 cgttctcgac cggctcgacg gctacacctt cgccatcggc gccgcagcgc
//
LOCUS       DAOMXG010000080        10377 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_3429_length_10377_cov_11.038074, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000080 DAOMXG010000000
VERSION     DAOMXG010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 10377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10377
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3429_length_10377_cov_11.038074"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..1572)
                     /gene="guaB"
                     /locus_tag="PLQ11_06915"
     CDS             complement(82..1572)
                     /gene="guaB"
                     /locus_tag="PLQ11_06915"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003602509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HRK24668.1"
                     /translation="MARIVPAAIPEALTFDDVLMLPAESSVLPNQTDLRTRLTKSIRL
                     NLPILSSAMDTVTEARLAIAMAQAGGIGVVHRNLTPEEQADHVRQVKRFESGMVMNPV
                     TIGPDAALREALDIMRARSISGLPVVEGESGRGGKLVGILTNRDVRFAENLDQPVREL
                     MTHSGLVTVRETVTPEEARHLFHKHRIEKLLVVDDDYNCVGLVTVKDIEKARLNPNAC
                     KDEQGRLRVAAATTTGDDGFRRSELLIDAGVDVLVVDTAHGHSRGVLAQVAKVKTLSN
                     KVQVIAGNVATADGARALIDAGADAVKVGIGPGSICTTRIVAGVGVPQLTAIMETARA
                     AHDQGIPIIADGGIKYSGDLAKAIAAGADVAMIGSLLAGTDESPGEVFLYQGRSYKSY
                     RGMGSLGAMARGSADRYFQAEIKDTMKLVPEGIEGQVPYKGPVSGVLHQLAGGLKAAM
                     GYVGAATIAELQERAQFVRISSAGLRESHAHDVTITRESPNYPTRM"
     gene            complement(1734..2192)
                     /locus_tag="PLQ11_06920"
     CDS             complement(1734..2192)
                     /locus_tag="PLQ11_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009468712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRK24669.1"
                     /translation="MDRIDLQILARLQQDSNQPLAQLAQAVGLSASACHRRIKLLEEA
                     GAIGGYAARLDRTALGLTMELYVTVSLISQSNEALEGFERAVQSVPEILSCALLAGRA
                     DYLMHIAVRDMDDFERIHRQKLSRLPGVSTMVTSFSLRRVKEFRGYPVGR"
     gene            2325..3440
                     /gene="ald"
                     /locus_tag="PLQ11_06925"
     CDS             2325..3440
                     /gene="ald"
                     /locus_tag="PLQ11_06925"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014131201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HRK24670.1"
                     /translation="MLVGVPKEVKNHEYRVGMTPESVAEMVHHGHKVIVETRAGAGIG
                     ATDKAYRAAGAEVVAEAAEIFARADMIVKVKEPQPFECERLRPGQILYTYLHLAPDPE
                     QTRALIKSGAICIAYETVTDESGGLPLLKPMSQVAGRMAVHAGANALEKAHGGRGVLI
                     GGVPGVAPAKVVIIGGGVVGFNAAQMAVGMHADVVILDRNPVVLERLSTHFEASATVV
                     YAMKSAIEHHVSQADLVIGAVLVPGAAAPRLVTRQMIRGMKKGSVLVDVAIDQGGCFE
                     TSRPTTHAKPTYVVDGVVHYCVANMPGAVARTSTYALNNVTLQHALAIADKGWKKALS
                     DNRHLANGLNVCAGKVTYEAVARDLGYDYVPAARMLG"
     gene            3559..4482
                     /locus_tag="PLQ11_06930"
     CDS             3559..4482
                     /locus_tag="PLQ11_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006471258.1"
                     /note="LivHMGF is the membrane component of the LIV-I/LS
                     branched-chain amino acid transporter; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease LivH"
                     /protein_id="HRK24671.1"
                     /translation="MEVFVQQVINGLTLGAIYGLIAVGYTMVFGIIGMVNFAHGDVFM
                     IGGFTALILFILVTSVVGLSAGAIFLAILVCLALGMALTGLWNWAIERIAYRPLRGSF
                     RLAPLISAIGMSMVLVNFVQVTQGARNKPVPPILRDVIVLMEKDGFAVTLAYKQIVIF
                     VVTALLLGIFWYVVQKTPLGRAQRACEQDGKMAALLGINVDRTISITFVIGAALAAVA
                     GVMYLVYYGVISFSDGFIPGVKAFTAAVLGGIGSLPGAVLGGLIIGMVETLWSAYFSI
                     DYKDVAAFMLLAITLIFLPQGLLGKPEVEKV"
     gene            4482..5849
                     /gene="livM"
                     /locus_tag="PLQ11_06935"
     CDS             4482..5849
                     /gene="livM"
                     /locus_tag="PLQ11_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006467801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivM"
                     /protein_id="HRK24672.1"
                     /translation="MAATSDDQPASFADNVKNALFWTAIAFGLFILLIGLKTDQQANS
                     IVLEPRPMLLFGVLASVFIATLAGNMLISQRTSARLKPSSVAAGPSWLSRVEPVLKPF
                     LLAFVLVFPFVSIALAGWQGSIKWIDNFGIQILIYVMLGWGLNVVVGLAGLLDLGYVA
                     FYAVGAYSYALLATKFGLSFWILLPVAGILAAFWGIILGFPVLRLRGDYLAVVTLAFG
                     EIIRLVLINWVDFSNGYAGISSIPRPTLFGIPFTSGDDGFAARFGLEPSGLYYKIFLF
                     YLILFLALITAWVTTKLRRLPIGRAWEALREDEIACRALGINTTTTKLTAFAIGAMFG
                     GFGGSFFAARQGFISPESFTFIESAQILAIVVLGGAGSLYGVVIAAVGIVGGFEILRE
                     LDFLKAVFGNDFDPAQYRALIFGLSMVVIMLWKPRGLVSSREPSVALKERKAISTDLV
                     KEGHG"
     gene            5849..6757
                     /locus_tag="PLQ11_06940"
     CDS             5849..6757
                     /locus_tag="PLQ11_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006935271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRK24673.1"
                     /translation="MSLVSSPLLTVEHMTMRFGGLVAINDLSFVVNSGEITALIGPNG
                     AGKTTVFNCITGFYKPTVGRLALRLGNGDFDGLVDEATAGADAVAGNADAMLYLLERM
                     PDYEITQKARIARTFQNIRLFSGMTVLENLLVAQHNALNAASLYTFGGLFGIPSFKVA
                     EKESIEKAKYWLERTGLIDRADDPAGDLSYGAQRRLEIARAMCTNPVLLCLDEPAAGL
                     NPKESAELNALIRSIRDQHECSVMLIEHDMSVVMEISDHVVVLDYGTKIADGTPDEIK
                     ADPRVIAAYLGVADEEVDEVEAEVGL"
     gene            6754..7497
                     /locus_tag="PLQ11_06945"
     CDS             6754..7497
                     /locus_tag="PLQ11_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRK24674.1"
                     /translation="MTTAASTATPMLQVRGVKTYYGNIIALKGVDLDVNEGEIVTLIG
                     ANGAGKSTLMMTIFGKPRAREGTIRYRGQDITSMPTHEIARLGINQSPEGRRIFPRMT
                     VYENLQMGAAVDDFRHFDQDLERVTGIFPRLKERLHQRGGTLSGGEQQMLAIARALMA
                     RPKLLLLDEPSLGLAPIIVKQIFEVIKEINQKDGMTVFLVEQNAYHALRLAHRAYVMV
                     NGVITMSGEGKVLLKDPQVRAAYLEGGHH"
     gene            7497..7844
                     /locus_tag="PLQ11_06950"
     CDS             7497..7844
                     /locus_tag="PLQ11_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24675.1"
                     /translation="MQGILYEESSAWLFVLVTVVMGGAAAWQTGRAIASVWKPQLLLI
                     GYMLLLGAAVRFTHFALFEGTLLSLRFYIVDTVIVTAIALIGWRIARSQQMLRQYDWL
                     YEANGPGRWKRKG"
     gene            7969..9090
                     /locus_tag="PLQ11_06955"
     CDS             7969..9090
                     /locus_tag="PLQ11_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006725834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HRK24676.1"
                     /translation="MKKTLLAGVALAAGLMMASAANAQIKFGVAGPITGPNAAFGKQL
                     TDGVEQAVEDINAAGGINGQKIALSKGDDVSDPKQGVSVANKFAADGVKFVVGHFNSG
                     VSIPSSEVYAENGILAITPASTNPKLTENPKASWNVFRTCGRDDQQGEVAGAYVADKL
                     KGKKVAVVHDKTTYGKGLADETKKAMNAKGIKEVVYEGVNTGEKDFSALVSKLKAAGV
                     EVVYWGGLHTEGGLIVRQMRDQGLNAVMMSGDGITSAEFATIGGPGVEGTLMTFPPDP
                     RGRPEAAKVVAAFKAKKIDPESYTLYSYAAVEVIKQAADATKSLDPKKVAEHMKSGAK
                     FKTVIGELSFDKKGDITRPDYVMYTWKKGPDGKISYFQN"
     gene            complement(9179..9907)
                     /locus_tag="PLQ11_06960"
     CDS             complement(9179..9907)
                     /locus_tag="PLQ11_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HRK24677.1"
                     /translation="MTDLSIPYVAFAGLLSFLSPCVLPLVPPYLCYLAGTTLDEWTDE
                     NESGINRMALMAAFLFVLGFSTVFVLLGAGASVIGAVLRQNSLELGWLAGAIIIIMGL
                     HFMGVIRIAFLAREARMHVEKPAGLAGAYVMGLAFAFGWSPCIGPVLGSVFAVAGSEA
                     SVTRGMALLAVYSAGLGIPFLVAAFAMGPFVRFLRRYRAHIGKVEKVMGVLLVATGIA
                     FLTGSFTILGTWLLEAFPVLGKLG"
     gene            10004..>10377
                     /locus_tag="PLQ11_06965"
     CDS             10004..>10377
                     /locus_tag="PLQ11_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007798333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HRK24678.1"
                     /translation="MRNLITDVHGVMVGNADDVRLRSGVTVIAFAEPAVASVCQRGGA
                     PGNRDIGLLDPAMTVEKVDAIVLSGGSAFGLDAAGGVQSVLREQGRGFPVGDLCVPIV
                     PQAIVFDLLNGGLRFGVEVIHRT"
BASE COUNT         1879 a         3294 c         3261 g         1943 t
ORIGIN      
        1 agccacttac aaacctgcga agcgacgccg agcgagtgcg gggggcgcga gcttcagcgc
       61 acccctacaa aaccttcatc cttacatccg cgtcggatag ttcgggcttt cgcgggtgat
      121 cgtcacgtca tgggcgtggc tttcgcgcag gcccgccgag gagatgcgga cgaactgcgc
      181 ccgctcctgc aattccgcga tcgtcgcggc gccgacatag cccatcgccg ccttgaggcc
      241 gccggcgagc tgatgcagca cgcccgacac cggccccttg tagggcacct gcccttcgat
      301 gccctccggc accagcttca tcgtatcctt gatctcggcc tggaagtagc ggtcggccga
      361 gccgcgcgcc atcgctccca gcgaacccat gccgcgatac gacttgtagg agcggccctg
      421 atacaggaat acctcgccgg ggctctcgtc cgtgccggcc agcagcgagc cgatcatcgc
      481 cacatcggcg ccggcggcaa tcgccttggc cagatcgccc gaatacttga tcccgccatc
      541 ggcgatgatc gggatgccct ggtcatgggc tgcgcgcgcg gtctccatga tcgccgtgag
      601 ctgcggcaca ccgacaccgg cgacaatccg cgtcgtgcag atcgaccccg gaccgatgcc
      661 gaccttgacc gcatccgccc ccgcatcgat cagcgccctg gcgccatcgg cggtagccac
      721 attgccggcg atcacctgca ccttgttcga cagcgtcttg accttggcta cctgcgccag
      781 cacacccctg gaatggccat gcgcggtatc gacgaccagc acatcgacgc cggcatcgat
      841 cagcagttcc gagcggcgga acccatcgtc acccgtggtc gtcgcggcgg ccacgcgcag
      901 ccgcccctgc tcgtccttgc aggcattggg gttgagcctc gccttctcga tatccttgac
      961 cgtgacgagg ccgacgcagt tgtaatcatc gtcgaccacc agcagcttct cgatgcggtg
     1021 cttgtggaac aggtgccgcg cctcctccgg cgtgacggtc tcgcgcacgg tgacgagccc
     1081 cgaatgggtc atcagctcgc gcaccggctg gtctaggttt tcggcaaagc gcacatcgcg
     1141 gttggtgagg atgccgacca gcttgccgcc gcgcccgctt tcaccttcga ccaccggcag
     1201 gcccgaaatc gaccgcgccc gcatgatgtc cagcgcctcg cgcagggcgg catccggacc
     1261 gatggtcacc ggattcatca ccatgccgct ctcgaagcgc ttgacctgcc gcacatggtc
     1321 ggcctgttcc tccggcgtca ggttgcgatg gacgacgccg atgccgccgg cctgtgccat
     1381 ggcgatcgcc agccgcgcct cggtcacagt atccatcgcc gaggacagga tcggcagatt
     1441 gagccggatc gacttggtca gcctggttct gaggtcagtc tgattgggca gcaccgacga
     1501 ttcggccggc aacatcagca cgtcatcgaa cgtcagtgct tcaggaatcg cggcgggaac
     1561 gatgcgggcc atgccaaacc tcttcagaat acccgtccgg atcgctgcaa acgcgatggg
     1621 caagggacgg ggcaacagga attggcgctg gctggtaaca cgcccccgga cagggggaaa
     1681 ggaaaatccg tcaaagccgg tcgctgatgc agggcgaggc cgcgccgcca gcttcatcgc
     1741 cccaccggat agccgcggaa ctccttcacc cgcctgaggc tgaaactggt caccatcgtc
     1801 gaaacccccg gcaggcgtga cagtttctgc cggtggatgc gctcgaaatc atccatatcg
     1861 cgcacggcaa tgtgcatcag gtaatcggcc cggcccgcca gcagcgcgca ggacaggatc
     1921 tccggaacgc tctgcaccgc ccgctcgaac ccttccagcg cctcgttcga ctgcgagatc
     1981 agcgaaaccg tgacgtagag ttccatggtc agccccagcg ccgtccggtc gagacgcgcc
     2041 gcataaccgc cgatcgcccc cgcctcctca agcagcttga tgcgccgatg gcaggccgag
     2101 gctgacaggc caacagcctg cgcaagctgc gccagcggct ggtttgaatc ctgttgcaga
     2161 cgcgccagga tttgcagatc aattcggtcc atcgaatatt ttccggattt tgtcacgaaa
     2221 cttcgaattt catatcacac gggcgccgcc tgaccagaca gatcgcaatg caattccatt
     2281 gcgctccgac tacagtctac aaaaatcacc agcggaggat gaccatgctc gtcggcgttc
     2341 ctaaggaagt gaagaaccac gagtatcgcg tcggcatgac gcccgagagc gttgccgaga
     2401 tggttcatca cggccacaag gtcatcgtcg agacgcgcgc cggtgccggc ataggcgcca
     2461 ccgacaaggc ctatcgcgcc gccggcgccg aagttgtcgc cgaagccgcc gagatcttcg
     2521 cccgcgccga catgatcgtg aaggtcaagg agccgcagcc gttcgaatgc gagcgcctgc
     2581 gccccgggca gatcctctac acctacctgc atcttgcgcc cgacccggaa cagacccggg
     2641 cgctgatcaa gtccggcgcg atctgcatcg cctacgagac cgtcaccgat gagtccggcg
     2701 gcctgccgct gctcaagccg atgagccagg tggccggtcg catggctgtg catgccggcg
     2761 ccaatgcgct tgagaaggcc catggcggtc gcggcgtgct gattggcggc gtgccgggcg
     2821 ttgcaccggc caaggtggtg atcatcggcg gcggcgttgt tggtttcaac gcggcgcaga
     2881 tggccgtcgg catgcacgcc gatgtggtca tcctcgaccg caacccggtg gtgcttgagc
     2941 gcctgtcaac ccatttcgag gcttcggcaa cggtggtcta tgcgatgaaa tcggcgatcg
     3001 agcaccatgt cagccaggcg gatctggtga tcggcgccgt gctggttccc ggcgcggccg
     3061 cgccccggct agtgacccgc cagatgatca ggggcatgaa gaagggctcg gttctggtcg
     3121 atgtggccat cgatcagggc ggctgctttg aaacgtcgcg gccgaccacc catgccaagc
     3181 cgacctatgt ggtcgacggc gtcgtgcatt attgcgtcgc caacatgccc ggtgcggttg
     3241 cccgcacctc gacctatgcg ctgaacaatg tcacgctcca gcacgcgctg gcgatcgccg
     3301 acaagggctg gaagaaggcg ctgagcgaca accgccatct tgccaacggc ctcaacgtct
     3361 gcgccggcaa ggtgacctat gaggcagttg cccgcgacct cggttatgac tatgtgccag
     3421 cagcccgcat gctgggctag ccaacaaatt gggcagttgc cgcacaaagg ggcttgcgca
     3481 agagcgaatt gataaagaaa agttgtgacc gccatcacgt ggcagaatgc caggacggcc
     3541 aggggaggcg tatagcgtat ggaagtcttt gttcagcagg tcatcaacgg gctgacgctc
     3601 ggggcgatct atgggctgat tgccgtcgga tatacgatgg ttttcggcat catcgggatg
     3661 gtcaacttcg cccatggcga tgtgttcatg atcggcgggt tcaccgccct gatcctgttc
     3721 attctcgtga ccagtgtggt cggattgagc gcgggggcga tctttctcgc cattctggtc
     3781 tgtcttgcgc tcggcatggc gctgaccggt ctgtggaact gggcgatcga gaggattgcc
     3841 taccggccct tgcgcggctc gttccgtctg gcgccactga tctcggcgat cggcatgtcg
     3901 atggttctgg tcaatttcgt gcaggtgacc cagggcgccc gcaacaagcc ggtgccgccg
     3961 atcctgcgtg atgtgatcgt gctgatggag aaagacggtt ttgccgtcac cctcgcctac
     4021 aagcagatcg tgattttcgt ggtcacggcg ctgctgctcg gcatcttctg gtatgttgtg
     4081 cagaagaccc cgcttggccg ggcccagcgc gcctgtgagc aggacggcaa gatggcggcg
     4141 ctgctcggca tcaacgtgga tcgcaccatc tcgatcacct tcgtgatcgg tgcggcactc
     4201 gcggcggtcg cgggcgtgat gtatctggtc tactacggtg tgatttcctt ctcggatggc
     4261 ttcattcccg gcgtcaaggc attcaccgct gcggttctgg gcggcatcgg ctcgctgccc
     4321 ggcgcggtgc tcggcgggct gatcatcggc atggtcgaaa ccctgtggtc ggcctatttc
     4381 tcgattgatt acaaggatgt ggctgccttc atgctgcttg cgatcacgct gatcttcctg
     4441 ccgcagggcc tgctcggcaa accggaagtg gagaaggtgt gatggctgcc acctccgacg
     4501 accagccggc gagcttcgcc gacaatgtga aaaacgcgct gttctggacg gccatcgcct
     4561 ttggcctgtt catcctgctg atcggcctca agactgacca gcaggccaat tccatcgtgc
     4621 tggaaccgcg cccgatgctg ctgttcggcg ttctggcgag cgtgttcatt gccacgctcg
     4681 ccggcaacat gctgatttcg cagcgcacca gcgcgcgcct gaaaccctcg tccgtggcgg
     4741 ccgggccttc gtggctcagc cgtgtcgagc cggtgctcaa gccgtttctt ctggccttcg
     4801 ttctggtctt tccattcgtt tcgatcgcgc tggcaggctg gcagggatcg atcaagtgga
     4861 tcgacaattt cggcatccag atcctgatct atgtgatgct tggctggggg ctcaacgtgg
     4921 tggtcggcct ggccggcctg ctcgatctcg gctatgtcgc gttctacgcg gtcggggcct
     4981 attcctacgc gcttctggcg accaaattcg gcctgtcctt ctggatcctg ctgccggtcg
     5041 ccggcattct ggcggccttc tggggcatca tcctgggctt tccggtcttg cgactgaggg
     5101 gcgattatct ggcggttgtg acgctggctt ttggtgagat catccgcctt gtgctgatca
     5161 actgggtgga cttttccaac ggctatgccg gcatatcctc gatcccgcgg ccgacgctgt
     5221 tcggcattcc cttcaccagc ggcgacgatg gttttgccgc ccgtttcgga ctggaaccgt
     5281 ccgggctcta ctacaagatc ttcctgttct atctgatcct gttcctggcc ctgatcaccg
     5341 cctgggtgac gaccaagctg cgccgcctgc cgatcggccg ggcgtgggaa gcgttgcgtg
     5401 aggatgagat cgcctgccgc gcgctcggca tcaacaccac gaccaccaag ctgacggcct
     5461 ttgccatcgg cgcgatgttc ggcggcttcg gcggctcgtt ctttgcggcc cggcaggggt
     5521 tcatctcgcc ggaaagcttc accttcatcg aatcggcgca gattctggcc atcgtcgtgc
     5581 ttggcggcgc cggttcgctc tatggggtgg tgatcgcggc ggtcggcatc gtcggcggct
     5641 ttgaaatcct gcgcgaactc gacttcctga aggcggtgtt cggcaatgat ttcgaccctg
     5701 cgcaataccg cgcgctgatt ttcggcctgt cgatggtggt gatcatgctt tggaagccgc
     5761 gcggactggt ctcctcgcgt gaaccatcgg tggcgttgaa ggaacgcaag gccatctcga
     5821 cggatttggt caaggagggc cacggctgat gtcgctcgtt tcctcccccc tgctcaccgt
     5881 cgagcacatg accatgcgct ttggcggcct ggtcgccatc aatgacctgt ctttcgtcgt
     5941 caacagcggc gagatcacgg cgctgatcgg cccgaacggc gccggcaaga cgactgtgtt
     6001 caactgcatc accggctttt acaagcctac tgtggggcgg ctggcgctcc gtctcggcaa
     6061 cggtgacttc gacggcctgg tcgacgaagc cacggctggc gccgatgctg tggccggtaa
     6121 tgcggatgcc atgctgtatc tgcttgagcg catgccggat tacgagatca cgcagaaggc
     6181 acggattgcc cgcacgttcc agaatatccg cctgttttcg ggcatgaccg tgctcgagaa
     6241 cctgctcgtg gcccagcaca acgcactcaa tgccgcctcg ctctacacct ttggcggcct
     6301 gttcggcatt ccctcgttca aggttgccga gaaggagagc atcgagaagg cgaaatactg
     6361 gctggagcgc accggtctga ttgaccgcgc cgacgatccg gccggcgatc ttagctatgg
     6421 cgcgcagcgg cggctggaaa ttgcccgtgc catgtgcacc aatccggtcc tgctctgcct
     6481 tgacgagccg gcggccggcc tcaaccccaa ggaaagcgcc gaactcaacg cgctgatccg
     6541 ttcgatccgt gaccagcacg agtgttcggt gatgctgatc gagcacgaca tgtcggtggt
     6601 catggaaatt tcggatcatg tggtggtgct tgattacggc accaagattg ccgatggcac
     6661 gcccgacgag atcaaggccg acccgcgggt gatcgcggcc tatctcggcg tcgccgacga
     6721 ggaagtggac gaagtggaag cggaggtcgg cctatgacaa ccgcagccag cacagccacg
     6781 ccgatgctgc aggtgcgcgg cgtcaagacc tattacggca acatcatcgc actcaaaggc
     6841 gtcgatctcg acgtcaacga gggcgagatc gtcacgttga ttggcgccaa cggcgccggc
     6901 aagtcgacgc tgatgatgac cattttcggc aagccgcgcg cccgtgaggg caccatccgt
     6961 tatcgcggac aggacatcac cagcatgccg acgcacgaga tcgcgcggct gggcatcaac
     7021 cagtcaccgg aagggcggcg gattttcccg cgcatgacgg tatacgagaa cctgcagatg
     7081 ggggcggctg tcgatgactt ccgccatttc gaccaggatc tcgaacgggt gaccggcatt
     7141 ttcccgcgcc tcaaggagcg ccttcaccag cgcggcggca cgctttcggg cggcgagcag
     7201 cagatgctgg cgatcgcccg cgcgctgatg gcccgcccca agctgctgct cctggacgaa
     7261 ccgtcgctcg ggcttgcccc gatcatcgtc aagcagatct tcgaggtgat caaggagatc
     7321 aaccagaagg acgggatgac ggtgtttctg gtcgaacaga acgcctatca tgcgttgcgt
     7381 ctggcccatc gcgcctatgt catggtcaac ggcgtcatca ccatgtccgg cgaaggcaag
     7441 gtcttgctga aggatccgca ggtccgcgcc gcctatcttg aaggaggaca ccactgatgc
     7501 agggaatcct ctacgaagaa tcctcggcct ggctgtttgt tctggtcacg gtggtcatgg
     7561 gtggcgctgc ggcctggcag accggccgcg ccatcgccag cgtatggaag ccgcagttgc
     7621 tgctgatcgg ctacatgctg ctgctgggag cggcggtgcg gttcacgcat ttcgccctgt
     7681 tcgagggcac tttgctgagt ttgcgcttct acatcgtcga tacggtcatc gtcaccgcca
     7741 tcgcgttgat cggctggcgc atcgcgcgca gccagcagat gctgcgacaa tatgactggc
     7801 tgtatgaggc gaacggcccg ggacgctgga agcgcaaggg ctgagcatcc atttttgtgg
     7861 tgacagacag caagaaacgc gatttgttgg agacaaggcg ccgcacggcg cccaaaccgc
     7921 atgcgggggc ggcagactcc gcgaactgat gcatacggga gatgatgcat gaaaaagact
     7981 cttctggcgg gcgtcgccct ggccgctggc ctgatgatgg cgagcgcggc caacgcccag
     8041 atcaagttcg gcgttgccgg accgatcacc gggccgaacg ctgcattcgg caagcagctg
     8101 accgatggtg tcgagcaggc ggttgaggac atcaacgccg ccggcggcat caacggtcag
     8161 aagatcgccc tgtccaaggg cgatgacgtg tccgatccca agcagggcgt gtctgtggcc
     8221 aacaagttcg ctgccgatgg cgttaagttc gtggtcggcc acttcaactc gggcgtgtcg
     8281 atcccgtcct cggaagtcta tgccgagaac ggcatcctgg cgatcacccc ggcctcgacc
     8341 aacccgaagc tgaccgagaa cccgaaggcg agctggaacg tgttccgcac ctgcggtcgt
     8401 gacgaccagc agggcgaagt cgccggcgcc tatgtcgccg acaagctcaa gggcaagaag
     8461 gtcgccgtgg tccatgacaa gaccacctac ggcaagggcc tcgccgacga gaccaagaag
     8521 gcgatgaacg ccaagggcat caaggaagtc gtctacgaag gtgtgaacac cggcgagaag
     8581 gacttctcgg cgctcgtttc caaactcaag gcagccggcg ttgaagtcgt ctactggggc
     8641 ggcctgcaca ccgaaggtgg cctgatcgtc cgccagatgc gcgaccaggg cctcaacgcg
     8701 gtgatgatgt cgggtgacgg catcacctcg gccgagttcg ccaccattgg cggcccgggc
     8761 gtcgaaggca cgctgatgac cttcccgccg gatccgcgcg gccgtccgga agcagcaaag
     8821 gtggttgctg ccttcaaggc caagaagatc gacccggaga gctacacgct ctattcctac
     8881 gctgcggtcg aggtgatcaa gcaggcggcc gatgcgacca agtcgctcga ccccaagaag
     8941 gtcgccgagc acatgaagtc cggcgccaag ttcaagaccg tgatcggcga actgtccttc
     9001 gacaagaagg gcgacatcac ccgtccggac tatgtgatgt atacctggaa gaagggcccg
     9061 gacggcaaga tctcctactt ccagaactga tcggctttgc cggcggacgg gcgccctgcc
     9121 cgtccgtttc cagtcttgca ccccgccagc ggttcaaccc cggcggggtg tttttttgtc
     9181 agccgagctt gccgagaacc gggaaggctt ccagcagcca ggtccccaga atggtgaagg
     9241 agccggtcag gaaggcgata ccggttgcca ccagcaaaac gcccatcacc ttctcgacct
     9301 tgccgatatg cgcacggtag cggcgcagga agcggacgaa cggccccatg gcgaaggccg
     9361 ccaccagaaa cgggatgccg agcccggccg aatagaccgc cagcagcgcc atgccgcgcg
     9421 tcacgctcgc ctccgagccg gccaccgcga acacgctgcc cagcaccggg ccgatgcacg
     9481 gcgaccagcc gaaggcgaag gccagcccca tcacataggc gccggccagt ccggccggct
     9541 tttccacatg catccgcgcc tcgcgcgcca ggaaggcgat gcggatcacg cccatgaaat
     9601 gcagccccat gatgatgatg atcgcgccgg cgagccagcc aagctccagc gagttctgcc
     9661 gcagcaccgc accgatcacc gaagcgcccg cccccagcag cacgaacacg gtggaaaagc
     9721 cgaggacgaa caggaacgcg gccatcagcg ccatgcgatt gatgccactc tcgttctcgt
     9781 cggtccattc gtccagcgtg gtgccggcca gatagcacag atagggcggc accagcggca
     9841 gcacgcacgg gctgaggaag ctcagcaacc cggcgaaggc aacgtagggg atggatagat
     9901 cggtcatgcg tggggtcccg acaagtctgt tgaccccctc gccctcacgg cggaaggatg
     9961 tctgaggctc tgcttggccc taccatgatc gaaaggcaaa atcatgcgca acctgatcac
    10021 ggatgttcac ggtgtcatgg tcgggaatgc agatgatgtt cggctgagga gcggagttac
    10081 ggtgatcgcc ttcgccgaac cggccgtcgc ctccgtctgc cagcgcggcg gtgcgcccgg
    10141 caatcgcgat attggcctgc tcgatccggc aatgaccgtc gagaaggtgg acgccatcgt
    10201 tctgtcgggt ggatcggcct tcgggctgga tgccgccggc ggcgtgcagt cggtgctgcg
    10261 cgaacagggg cgcggctttc ctgtcggaga tctctgcgtg ccgatcgtgc cgcaggccat
    10321 cgtctttgat ctcctcaacg gcggtctgcg cttcggtgtt gaagttatac atcgcac
//
LOCUS       DAOMXG010000081        10112 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_3528_length_10112_cov_9.172218, whole genome shotgun sequence.
ACCESSION   DAOMXG010000081 DAOMXG010000000
VERSION     DAOMXG010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 10112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10112
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3528_length_10112_cov_9.172218"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="PLQ11_06970"
     CDS             complement(<1..314)
                     /locus_tag="PLQ11_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRK24679.1"
                     /translation="MAARLSPVIILVRPQMAENIGMAARAMANFGLAEMRLVAPRDGW
                     PQKTRMKKGAHQAASGATHILEQAALFDTTAEAIADLNFVFATTARERGQAKPVLTPA
                     EG"
     gene            390..1232
                     /locus_tag="PLQ11_06975"
     CDS             390..1232
                     /locus_tag="PLQ11_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24680.1"
                     /translation="MNVEKTILPCRLLNFSGVIRVRRYITSAVMCLGLAGCVLDNRLD
                     LTAAGERADKGVVFMAIAASCPLPSVSLVARQGEDRRAINAMPGFFAVNGKSGLLQIN
                     LRDNVSVARFDIPPGTYQLERVDCYITNGQRLLQRPNSRNGIASFTVGPREILNLGTF
                     HEAPVIRPVNSIFGNYQRHTGQMMFNFTPMSGEEMAKLREKYPEEAAKAVYRPMSMDQ
                     EHQREHAVTLCTMDALLKRNDSAKPSVEETNALIAACKQQGHVPRPASAEPGKTQKKA
                     HRQS"
     gene            1393..2607
                     /locus_tag="PLQ11_06980"
     CDS             1393..2607
                     /locus_tag="PLQ11_06980"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017964507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HRK24681.1"
                     /translation="MSKIKVKNPVVEMDGDEMTRIIWDLIKKKLIHPYLDVNLLYYDL
                     SVEYRDATNDQVTIDSAEATKKHGVAVKCATITPDEARVKEFNLKEMWKSPNGTIRNI
                     LGGTIFREPIICKNVPRLVPGWTQPIVVGRHAFGDQYRATDFKFPGKGTLTIKFVGED
                     GTVIEKEVFKAPGAGVSLSMYNLDESIKDFARASFAYGLLRNYPVYLSTKNTILKAYD
                     GRFKDIFQAIFDAEFADRFKEKGLIYEHRLIDDMVASALKWSGGYVWACKNYDGDVQS
                     DVVAQGFGSLGLMTSVLMTPDGKTVEAEAAHGTVTRHYREHQKGRETSTNAIASIFAW
                     TRGLAHRAKLDNNPELARFCSTLEKVCVSTVESGHMTKDLALLVGPDQRWLSTTGFLD
                     KVDENLQKAMGV"
     gene            complement(2921..4147)
                     /locus_tag="PLQ11_06985"
     CDS             complement(2921..4147)
                     /locus_tag="PLQ11_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-gated ion channel"
                     /protein_id="HRK24682.1"
                     /translation="MRRRPQPVRESARHRVYQVLDGGGFGDAVSRRLHRLLIFLIVIN
                     VSSVVLETVPSLATTYATAFLAIEAASVAVFGLEYGLRLWVAPEHGPLADLSPWGARL
                     KLALTPSMVIDLLAILPFLLAFIVAADFRMLLVLRLFRFFKIARYSSGMRSLLNAVHA
                     ERNALMACALIQLGLILLSASFMHLVEHAAQPEKFGSIPSAMYWAVITLATVGYGDVV
                     PVTALGKVVASITALLGLGMVALPVGILANAFAEEIHRRDFVVTWAMVARVPLFQGLN
                     AASIAEIMQFLRSQTVQPGQVIVRRGEVAHSMYFIAAGEVEIDLPRHTVNLGIGEFFG
                     EIALLGDVKRTATARAIARSNLLVLDKADLQALMERNPDLAERIMKEAHGQLPEHAVR
                     KGGDLVREEINHIGKS"
     gene            complement(4152..6812)
                     /gene="alaS"
                     /locus_tag="PLQ11_06990"
     CDS             complement(4152..6812)
                     /gene="alaS"
                     /locus_tag="PLQ11_06990"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012753019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HRK24683.1"
                     /translation="MSGVNEIRSTFLNFFSRNGHEVVASSPLVPRNDPTLMFTNAGMV
                     QFKNVFTGQEQRPYSRAVTAQKCVRAGGKHNDLDNVGYTARHHTFFEMLGNFSFGDYF
                     KADAIDLAWRLVTQDFALAKDRLTVTVYHEDDEAFGLWKKIAGLPDDRIIRIPTSDNF
                     WAMGDTGPCGPCSEIFFDHGPHIPGGPPGSADQDGDRFIEIWNLVFMQFEQLPGGQRV
                     SLPRPSIDTGMGLERISAVLQGTHDNYETDMMRSIIGTIAELVSVDPNGAQKASHRVI
                     ADHLRASAFLVADGVLPSNEGRGYVLRRIMRRAMRHAQLLGALEPLMYRLVPVLIREM
                     GQVYGELRRAEALIAETLKLEENRFRRTLERGLALLDEETRGFGAGATLKGETAFRLY
                     DTYGFPLDLTQDALRARSIDVDLDGFAAAMARQKAEARAKWAGSGEAATETVWFGIRE
                     RVGASEFLGYDTETAEGVVTALVRDGVEVRSLDVGESGFVVLNQTPFYGESGGQVGDA
                     GRFTGEGLAVEVADTQKKLGDVFVHAVKVVAGTLKVGTALNLLVDPARRGAIRGNHSA
                     THLLHEALRHVLGDHVAQKGSLVSAERLRFDISHPKPITEGELQAVEALANDMVLQNG
                     SVVTRLMGVDEAIASGARALFGEKYGDEVRVVSMGRYADGAQAGKPYSVELCGGTHVR
                     RTGDIGLVTILTEAAVGAGVRRIEAMTGAAARRHLGEESRLLQEVAAVLRAPVGEAVE
                     RVTQLVEERRRLERELGEARKKLAMGGGGRGEDEVQRVGSVKLIAKAVPGIDVRDLKS
                     LADEGKKRLGSGVVVLVSVAEDGRAGLVVGVTDDLTGRISAVDLVRAGAIELGGKGGG
                     GRPDMAQAGGPNGSGAEAALGAVSRALAAL"
     gene            complement(6943..8028)
                     /gene="recA"
                     /locus_tag="PLQ11_06995"
     CDS             complement(6943..8028)
                     /gene="recA"
                     /locus_tag="PLQ11_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008129115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HRK24684.1"
                     /translation="MTQPALRLVEGSSMDKTKALDAALSQIERAFGKGSIMRLGKNQK
                     AIEIETISTGSLGLDIALGVGGLPKGRVVEIFGPESSGKTTLALHTIAEAQKKGGICA
                     FVDAEHALDPVYARKLGVELENLLISQPDTGEQALEIADTLVRSGAVDVLVVDSVAAL
                     TPKAEIEGEMGEVQPGLQARLMSQALRKLTASISRSNTMVIFINQIRMKIGVMYGSPE
                     TTTGGNALKFYASVRLDIRRIGAIKEREEVVGNTTRVKVVKNKVAPPFKQVEFDIMYG
                     EGVSKMGELIDLGVKAGIVEKSGAWFSFDSQRLGQGRENAKQFLRQSPEVANRIEQMI
                     RQNAGLIAERILEHGGAEPEDDGPPEM"
     gene            8175..8417
                     /locus_tag="PLQ11_07000"
     CDS             8175..8417
                     /locus_tag="PLQ11_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019903912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3297 family protein"
                     /protein_id="HRK24685.1"
                     /translation="MSDTPPDRLSVNPQSPHYNEAALARDVGVRFKGVEKHNVEEYCV
                     SERWIRVAAGPAKDRRGNPLTLRLSGDVEVWFKNEA"
     gene            complement(8564..8761)
                     /locus_tag="PLQ11_07005"
     CDS             complement(8564..8761)
                     /locus_tag="PLQ11_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HRK24686.1"
                     /translation="MTQNVGGIDRALRIIIGLVLLSLVFVGPQTAWGWVGIVPLATGL
                     IGWCPPYSLFGFNTCSVPKKD"
     gene            complement(8851..>10112)
                     /locus_tag="PLQ11_07010"
     CDS             complement(8851..>10112)
                     /locus_tag="PLQ11_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24687.1"
                     /translation="RKVEASGAVRLLGARIGGDLACDGGRFLRKGEDALNCDGMSVTG
                     ALFLREVEASGEVRLLGARIGGNLVCDGGRFLKKGEDALSCDRMNVAGDLFLRKVEAS
                     GAVRLLGARIGGNLSCNGGRFLNEDGRALTGDKMSVTGTLFLREGTTIAGTLDLASAE
                     VGGLVDDPACWPSEGKLILDGFRYARFGGATPATDRRGDWLMRQRRDHLGSSFRPQPW
                     KQAIRVLREMGHDTEARALAIRFEQQRRKAGVFRGMAGVLHDLYGAMAGYGHRPMLLM
                     RWMAAVWLTCALFYQNASDFGVMAPTNPLVFDNPAYTDCRPRAGSLTNWTNSGTCNGP
                     NEYTTFQPLLYSLDLILPLVDLQQDKDWAPMVTRARTSDPKGETEDFPLGHFIRVVMW
                     FEILFGWVASLMFVAILSGLIKKGDEE"
BASE COUNT         2025 a         3415 c         2895 g         1777 t
ORIGIN      
        1 atgccttcgg ccggggtcag caccggcttg gcctggccgc gctcgcgcgc cgttgtcgcg
       61 aacacgaaat tgagatcggc aatggcttcg gcggtggtgt cgaacaatgc ggcctgttcg
      121 agaatgtgcg tcgcgcccga ggcggcctga tgcgcgccct ttttcatccg cgttttttgc
      181 ggccagccat cacgcggggc gaccagccgc atctcggcga ggccgaaatt ggccattgcc
      241 cgcgccgcca tgccgatgtt ctcggccatc tgcgggcgaa cgaggatgat gacgggggag
      301 agacgtgcag ccatgcatgg tgagtgcaat ggctggccag gttcggtcaa gcagaatgcg
      361 ccctcagggt tgactggcaa cgattcttct tgaatgtcga gaagacaatt ttaccatgcc
      421 gcttactgaa tttttctgga gtaattagag tgcgaaggta cataacttct gctgtaatgt
      481 gtctggggct tgcaggttgc gtactggata accggcttga tcttacggcg gctggcgaac
      541 gggcggacaa gggcgtggtg ttcatggcca ttgccgcaag ctgtcccctg ccgtccgtca
      601 gccttgtggc aagacaaggt gaagatcgca gggcaatcaa cgccatgccg ggcttcttcg
      661 cggtgaacgg caaatccgga cttcttcaaa tcaacctgag ggacaatgtt agtgtcgcgc
      721 gttttgacat tccccccggc acgtatcaac tggaacgggt ggattgctac attaccaacg
      781 gtcaacgctt gctccagcgc ccgaattcac gcaatggaat tgcttccttt acggttgggc
      841 cacgagaaat tctcaatctc gggaccttcc acgaggctcc tgtcatccgc ccggtcaata
      901 gcatattcgg caattaccag cggcacaccg ggcagatgat gttcaatttc actccaatga
      961 gtggtgaaga gatggccaag ctcagggaaa aataccctga agaggcggcc aaggcggttt
     1021 accgcccgat gagcatggat caggagcatc agcgcgagca tgccgtcacc ctgtgcacga
     1081 tggatgccct gctgaaacgg aacgactctg ccaaaccctc ggtggaagaa acaaatgcct
     1141 tgatcgccgc ctgcaagcaa caggggcacg ttccaagacc ggcatctgcc gaaccaggaa
     1201 agacacaaaa gaaggcccat cgccagagtt gaaaattctt tggccgagcg catgccggcc
     1261 gttgcgaggc gaagccgtcc ttacgccttt cgccttgctt cccgctccgc cgccgcaatg
     1321 ctatagcaag gccaacggcg ccatccgggg gtcccggcag gcacgccaag ttggattgtg
     1381 acgaggaaac cgatgtcgaa aatcaaggta aagaaccctg tcgtcgaaat ggacggcgac
     1441 gagatgaccc ggatcatctg ggacctgatc aagaagaagc tgatccaccc ttaccttgat
     1501 gtgaacctgc tctactacga cctgtcggtt gagtatcgcg acgccaccaa cgatcaggtg
     1561 accatcgatt cggcggaagc caccaagaag catggcgttg ccgtcaaatg tgccacgatc
     1621 acgccggacg aagcccgcgt gaaggaattc aacctcaagg aaatgtggaa gtcgccgaac
     1681 ggcaccatcc gcaacatcct cggcggcacc atcttccgcg agccgatcat ctgcaagaac
     1741 gtgccgcgcc tcgttcccgg ctggacccag ccgatcgtcg tcggccgcca tgccttcggc
     1801 gaccagtatc gtgccaccga tttcaagttc cccggcaagg gcacgctgac gatcaagttc
     1861 gtcggcgagg acggcacggt gatcgagaag gaggtgttca aagcacccgg cgccggcgtc
     1921 tcgctgtcga tgtataacct cgacgaatcg atcaaggact ttgcgcgcgc ctccttcgcc
     1981 tacggcctgc tgcgcaacta tccggtctat ctgtcgacca agaacaccat cctgaaagcc
     2041 tatgacggcc gcttcaagga tatcttccag gcgattttcg acgccgaatt cgccgacagg
     2101 ttcaaggaga agggcctgat ctacgagcac cggctgatcg acgacatggt cgcctcggcg
     2161 ctcaagtggt ccggcggcta tgtctgggcc tgcaagaact acgacggcga cgtgcagtcg
     2221 gacgtggtgg cccagggctt cggctcgctc ggcctgatga cctcggtgct gatgaccccg
     2281 gacggcaaga ccgtggaggc cgaagccgct cacggcaccg tgacccgtca ctaccgcgag
     2341 catcagaaag gccgggaaac ctcgaccaac gcgatcgcct cgattttcgc ctggacgcgc
     2401 ggccttgccc atcgtgccaa gctcgacaac aacccggaac tcgcccgctt ctgctcgaca
     2461 ctggagaagg tctgcgtctc gaccgtcgag agcggccaca tgaccaagga tctggcgctg
     2521 ctggtcggtc cggatcagcg ctggctgtca accaccggct tcctcgacaa ggtcgacgag
     2581 aacctgcaga aggcgatggg cgtctgacac cgctgcaggc aaaggcatta taccagcggc
     2641 cctgacaatt gaccgttcgg ggcaattgtc aggcggctgg ttagagcatg tcgcgattaa
     2701 gtggataccg gttaatcgca gaagccatgc tctaacttat tgataccgat cacgttatcc
     2761 gcattttgat gattctatca aaatgcggcg tgatctagga gcgaatgcga cgccgagcag
     2821 ggccgaaggc ccggttgcga gggacggccc gggaaatgac cggcaggtca tttgtgaggg
     2881 ccaccggtat taaggaaggc ccggtctcga ccgggctttt ttatgacttg ccgatgtggt
     2941 tgatttcctc gcgcacgagg tcgccgccct tgcgcaccgc atgttccggc agctggccgt
     3001 gcgcctcttt catgatccgt tcggcaagat cggggttgcg ctccatcaac gcctgaagat
     3061 cggccttgtc gagcacgagc aggttgctgc gcgcgatcgc ccgcgccgtc gccgtgcgct
     3121 tgacgtcgcc cagcagcgcg atctcgccaa agaattcgcc aatgccgagg ttgaccgtgt
     3181 ggcgcggcag gtcgatttcc acctcgccgg ccgcgatgaa atacatggaa tgggccacct
     3241 ccccccgccg gacgatcacc tgccccggct gcaccgtctg cgagcgaagg aattgcatga
     3301 tctcggcgat cgaggccgcg ttgaggccct gaaacagcgg cacccgcgcc accatcgccc
     3361 aggtgacaac aaaatcgcga cgatggatct cctcggcaaa ggcattggcg agaatgccga
     3421 ccggcagcgc caccatgccg agcccgagca gcgcagtgat gctcgccacc accttgccga
     3481 gcgctgtgac cggcactaca tcgccatacc ccacggtcgc caaagtgatc accgcccaat
     3541 acatcgccga cggaatcgag ccgaactttt ccggttgcgc cgcatgctcc accagatgca
     3601 tgaaactcgc cgacagcagg atcaacccca gctgaatcaa cgcacaagcc atcagcgcat
     3661 tgcgctccgc atggaccgca ttcagcaggg agcgcatgcc ggatgaatag cgcgcgatct
     3721 tgaaaaaccg gaacagccgc agcaccagca gcatccggaa atccgcagcc acgatgaacg
     3781 ccagcagaaa cggcaggatc gccaggagat cgatcaccat gctcggcgtc aacgccagct
     3841 tcagccgcgc tccccagggc gacagatccg ccagcgggcc atgttcgggc gccacccaaa
     3901 gccgcaaccc gtattcgagc ccgaacaccg caacgctcgc tgcctcgatc gccagaaacg
     3961 ccgtggcata ggtcgtcgcc aggcttggca ccgtctcgag caccaccgag cttacattga
     4021 tcacgatcaa aaaaatcagc agacggtgca gccgccggct gacagcatcg ccaaaaccac
     4081 cgccatcaag cacctgatag acccgatggc gggcactttc ccggacgggc tggggccgcc
     4141 gcctcatcct cttacaacgc cgccagcgcc cggctgactg cccccagcgc tgcctcggcg
     4201 ccggaaccgt tcggcccgcc cgcctgagcc atatcggggc ggccgccgcc acccttgccg
     4261 ccaagttcga tggcacccgc gcggaccaga tccaccgcgc tgatgcgccc ggtcagatca
     4321 tccgtcacgc caacaacaag cccggcacga ccatcctcgg caacgctgac cagaacaaca
     4381 acgcccgagc caagccgctt cttgccctca tccgccaggc tcttgagatc acgcacatca
     4441 atgcccggca cagccttggc gatcagcttg accgagccga cccgctgtac ctcatcctca
     4501 ccgcggccgc cgccgcccat cgccagcttc ttgcgcgcct cgcccagttc ccgttccagc
     4561 cgccgccgct cctcgaccag ttgggtgacg cgctccaccg cctcgccaac cggcgcgcgc
     4621 aaaaccgccg cgacctcctg caacagccgg ctctcttcgc ccagatgacg ccgcgccgcc
     4681 gcaccggtca tcgcctcgat acgccgcacg ccggcaccca cggccgcctc ggtcagaatc
     4741 gtcaccaatc cgatgtcgcc ggtgcgccgc acatgcgtgc ccccgcacaa ctcgaccgaa
     4801 tagggcttgc cggcctgcgc gccatctgca tagcggccca tcgacacaac ccgcacctcg
     4861 tcaccgtatt tctcgccgaa cagtgcccgc gcgccggagg caatcgcctc gtcaacgccc
     4921 atcagacggg tcaccaccga gccattctgc aacaccatgt cgttggccag cgcctcgacc
     4981 gcctgcaatt ccccctcggt gatcggcttg ggatggctga tgtcgaaccg caaccgctcg
     5041 gcactcacaa gcgaaccctt ctgcgccaca tgatcgccca gcacatggcg cagcgcttcg
     5101 tgcagcagat gcgtcgcaga atgattgccg cgaatggccc cgcgccgcgc cggatcaacc
     5161 agaagattga gcgccgtgcc gaccttcagc gtgccagcaa ccactttcac ggcatgaaca
     5221 aacacatcgc caagcttttt ctgggtatcg gccacctcaa cagccaaccc ctcgccggtg
     5281 aagcgccccg catcacccac ctgtccgccc gactcgccat aaaacggcgt ctggttcagg
     5341 acaacaaacc cggactcgcc cacatcgagg ctgcgcacct cgactccatc gcggacaagc
     5401 gccgtcacaa ccccctcagc cgtctcggta tcataaccca ggaactcact ggcaccgacc
     5461 cgctcgcgga taccgaacca taccgtttcg gtggccgcct cgcccgaacc ggcccacttg
     5521 gcccgcgcct ccgctttctg ccgcgccatc gccgcagcaa atccatcgag atcaacgtcg
     5581 atcgaccgcg cccgcaatgc atcctgcgtc agatcaagcg gaaacccata ggtgtcatag
     5641 agccggaaag ccgtctcgcc cttcaaggtc gccccggccc cgaaaccccg cgtctcctca
     5701 tccagcaggg caagaccgcg ctccagtgtt cgacggaacc ggttctcctc aagcttcaac
     5761 gtctcggcga tcagcgcctc cgcccggcgc agctccccat aaacctggcc catttcccgg
     5821 atcagcaccg gcaccagccg atacatcaga ggctcgagcg cgcccagaag ctgggcatgc
     5881 cgcatcgccc gccgcatgat ccgccggagc acatagccgc gcccttcgtt tgacggcagc
     5941 accccatccg ccaccagaaa cgccgaagcc ctgagatgat cggcgatgac cctgtggctc
     6001 gccttctgcg caccgttcgg gtcgaccgac accagctcgg cgatcgtccc gatgatcgaa
     6061 cgcatcatgt cggtttcata attgtcgtgt gttccctgaa gaaccgcaga aatccgttcc
     6121 agccccatgc cggtgtcgat cgatggccgc ggcagcgaca cccgctgacc gcccggcaat
     6181 tgctcgaact gcatgaacac gagattccag atctcgatga accggtcacc atcctggtcg
     6241 gcagacccgg gagggccacc cgggatatgc ggaccatgat caaaaaagat ttccgaacag
     6301 ggaccgcagg gtcccgtatc gcccattgcc cagaaattgt ccgacgtcgg aatccggatg
     6361 atccggtcat cgggcaaccc ggcaattttc ttccaaagcc cgaaggcctc atcgtcttcg
     6421 tgatacacgg tcacggtcag acggtccttc gcaagcgcga aatcctgcgt caccagccgc
     6481 catgccagat cgatcgcatc ggccttgaag taatcaccga aggaaaaatt gcccagcatc
     6541 tcgaaaaacg tgtggtgccg ggccgtatag ccgacattgt cgagatcgtt gtgctttcct
     6601 ccggcacgaa cgcatttctg tgctgtcacc gcccggctgt aaggccgctg ctcctgaccg
     6661 gtgaagacgt tcttgaactg caccattccc gcattggtga acatcagcgt cgggtcattg
     6721 cgcggcacca gcggactgga ggcgacgacc tcatggccat tgcgggagaa gaaattcaga
     6781 aatgttgacc ggatctcgtt gacgccactc atgatgcctg accgcaaacg ctcgcttgat
     6841 ggggccctgc cgtcctgtcg agccggacgg catgcccgtg aacccatcgc tcttgtagag
     6901 acgcttcagc ggcatgtcca cgcccacgca aggctgtccc gctcacatct cgggcggacc
     6961 gtcatcctcc ggctcggcac cgccatgctc gaggatacgc tcggcgatca gcccggcatt
     7021 ctggcggatc atctgttcga tgcggttggc gacctccgga ctctgcctga ggaactgctt
     7081 ggcgttttcc cgcccctgcc ccagacgctg gctgtcgaac gagaaccagg ccccggattt
     7141 ctcgacgatc ccggccttga cgccgagatc gatcagctcg cccatcttcg aaacaccctc
     7201 gccatacatg atgtcgaact cgacctgctt gaacggcggc gccaccttgt tcttcaccac
     7261 cttgacgcgc gtggtgttgc cgacgacctc ctcgcgctcc ttgatcgcgc cgatgcggcg
     7321 gatgtcgagg cgaaccgagg catagaattt cagcgcgttg ccgcccgtcg tcgtctccgg
     7381 gctgccatac atcacgccga tcttcatgcg gatctggttg atgaagatga ccatggtgtt
     7441 ggaccgtgag atcgacgcgg tcagcttgcg cagcgcctgg ctcatcagcc gcgcctgcag
     7501 gccgggctgc acctcgccca tctcgccctc gatctcggcc ttgggcgtca gcgccgccac
     7561 cgaatcgacc accagaacat caaccgcgcc ggaccgcacc agagtatcgg cgatttccag
     7621 cgcctgttca ccggtatcgg gctgcgagat cagcagattc tcgagctcca cccccagctt
     7681 gcgcgcatag accggatcaa gcgcgtgttc ggcatcgaca aaggcgcaga tgccgccctt
     7741 cttctgcgct tcggcgatgg tgtgcaaggc aagcgtggtc ttgcccgacg attcaggccc
     7801 gaaaatctcg accacccgcc ccttcggcag cccgccgacc ccgagcgcga tatcaaggcc
     7861 gagcgatccg gtcgagatcg tctcgatctc gatggccttc tggttctttc ccagccgcat
     7921 gatcgagccc ttgccgaagg ctcgctcgat ctggctgagc gcggcatcaa gcgccttggt
     7981 cttgtccatg gatgatcctt caacgagcct gagtgcgggc tgagtcatgg tatggcatcc
     8041 ttatgcatgg ccaggagagt cgcttcaacg caataaatgt accgcaaatg ttctcttttt
     8101 caagttctat ttttgttccg gttgcgattt ttctccgcgc ggggaagaag gagtggtttt
     8161 tgagggagcc cgccatgtcc gacaccccgc ccgaccgtct ttcggtcaat ccgcagagcc
     8221 cgcattacaa tgaggcggcg ctggcgcgcg acgtcggcgt gcgcttcaag ggcgtagaga
     8281 agcacaatgt cgaggaatat tgcgtttcgg agcgctggat tcgcgtcgcc gccgggcctg
     8341 ccaaggaccg caggggcaat ccgctgacgc ttcggctcag cggcgacgtc gaggtctggt
     8401 tcaagaacga ggcgtgatca ggccagcggc ttcggggatg accgccaggt cagaccggaa
     8461 gaccgctggt taggagcgaa tgcgacgccg agcagggcca aaggcccggt tgcgaggaat
     8521 gcccgaggga tgaccaaacg gtcagaaccg agggccacca ggatcagtcc tttttcggca
     8581 ccgagcaggt gttgaagccg aacagcgaat agggcgggca ccagccgatg aggccggtgg
     8641 cgagcggcac gatgccgacc cagccccacg ccgtctgcgg gccgacaaag acgagcgaga
     8701 gcagcaccag accgatgatg atgcgcaggg cgcggtcgat accgccgaca ttctgggtca
     8761 cgggaacctc ctgttgaata agaatgtcct aatataatag gcgcgctacc gcccgtcacg
     8821 gagaatctgt ttgcaaatct gcaaacaccc ctactcctcg tcgcccttct tgatcaggcc
     8881 ggagaggatg gcgacgaaca tcaggctcgc cacccagccg aacaggatct cgaaccacat
     8941 cacgacgcgg atgaaatggc ctagcgggaa atcctcggtc tcccccttgg ggtcggaggt
     9001 cctggcccgc gtcaccatcg gcgcccagtc cttgtcctgc tgaagatcga ccagcggcag
     9061 gatcagatcg agcgaataga gcagcggctg gaaagtggtg tattcgttcg gtccattgca
     9121 ggtgccggag ttggtccagt tggtcaggct gccggcgcgc ggccggcagt cggtataggc
     9181 agggttgtcg aacaccagcg gattggtcgg cgccatgacg ccgaagtcag atgcgttctg
     9241 atagaacagc gcacaggtca gccagaccgc cgccatccat cgcatcagca gcatcggccg
     9301 gtggccatag ccggccatgg cgccataaag atcgtgcaga acgccggcca tgccccggaa
     9361 cacgccggcc ttgcggcgct gctgctcgaa ccggatcgcc agcgcccgcg cctcggtatc
     9421 atggcccatc tcgcgcagaa ccctgatcgc ctgcttccac ggctgcgggc ggaagctgct
     9481 gccgagatga tcccgtcgct ggcgcatcag ccagtcgccg cgccggtcgg tggcgggcgt
     9541 tgcgccgccg aagcgggcgt agcggaaccc gtcgaggatc agcttgccct cgctgggcca
     9601 gcaggcgggg tcatcgacaa gaccgcccac ctcggcggag gccagatcaa gcgtgccggc
     9661 gatcgtcgtg ccctcgcgca ggaacagagt gccggtgaca ctcatcttgt caccggtcag
     9721 cgcccttccg tcttcgttca ggaaccggcc gccgttgcac gagagatttc cgccgatccg
     9781 cgcgccgagc agccgcactg cgccgctcgc ctcgaccttg cgcaggaaca gatcgccggc
     9841 gacattcatc ctgtcacagc tcagcgcgtc ttcgcctttc ttgaggaacc ggccaccgtc
     9901 gcacaccaga tttccgccga tccgcgcgcc gagcagccgc acttcgccgc tcgcctcgac
     9961 ctcgcgcagg aacagcgcgc cggtgacact catcccgtca cagttcagcg cgtcttcgcc
    10021 tttcctcagg aaccggccgc cgtcgcacgc cagatcgccg ccgatccgcg cgccgagcag
    10081 ccgcactgcg ccgctcgcct cgaccttgcg ca
//
LOCUS       DAOMXG010000082        10085 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_3538_length_10085_cov_8.468295, whole genome shotgun sequence.
ACCESSION   DAOMXG010000082 DAOMXG010000000
VERSION     DAOMXG010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 10085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10085
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3538_length_10085_cov_8.468295"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2261
                     /locus_tag="PLQ11_07015"
     CDS             <1..2261
                     /locus_tag="PLQ11_07015"
                     /inference="COORDINATES: protein motif:HMM:NF025086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HRK24688.1"
                     /translation="DRKRQFALISDFLANRDGEAVWITDAVAIGETDEATQGFIAALG
                     SKLRIIAATTSPLRLIRDAANSRDAMVVKLQRLGALEGAAAGIVRASDSRGRTLGEAN
                     FAFATGNDTEARLAVPLEVRNDIARIEIADGRSAGAVWLLDAGAQRRKVAIISGETAD
                     TAQPLVSGTYFVTKALAPYATLSEPPRGGDALTRALEDRPDVIVLVDIATVAGPALAD
                     LEKFVEDGGVLIRFAGSGLVAGSDALLPVKLRRGGRVLGGALSWDKPQRLAAFTDTGA
                     FAGLPVPADVTIERQVLAEPEPTLNERTWAALGDGTPLVTAGRRGKGLIVLFHVTADT
                     GWSNLPLSGVFVDMLRKILPLAASTATRSDSGPERANPRRILDGFGIFGPPPPSAKPL
                     PRSHAGGASQDHPPGFYGPPEASIAVNTLAPETRVAPLDLAGATVRPLAARPPLDLRL
                     PLLALAVVLFLLDALAVLFLSGLLRRSALAAGAVALCLVLPGGPAVSPAQAQTPSAQD
                     VEASLKPRLAYIVTGNREVDETSRLGLTALTRTMAARTAMEPGAPIGLDPAKDELVFY
                     SLIYWPMASDQPMPSEAALRRIDQFMKNGGTVIFDTRDATAIASAAGTTAETRRLRQM
                     LSNLDIPQLEPIPRDHVITKTFYLLDKLVGRYAQGETWIEAMPRGLGDDKRPARAGDR
                     VSPIIITSNDLAAAWAVDRSGNPRFPMVPGEPRQREMAMRAGVNLVMYAMTGNYKADQ
                     VHVPALLERLGQ"
     gene            2263..4368
                     /locus_tag="PLQ11_07020"
     CDS             2263..4368
                     /locus_tag="PLQ11_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24689.1"
                     /translation="MSNHFSLSFDPFLPWPVLIGLALIALLLAGLLIWHGKRGGWTRL
                     AACALLIAALTDPRFVNEEREGLPEVVALLVDRSGSQGIGERAAQTDAASGELEQRIA
                     ARPNTTLRKIEVFERDSDVNGTHLFEGLSAALADVPPERLAGIVAITDGVVHDVPASL
                     RALGFGAPFHGLITGKAGEFDRRIEIVESPRFGIVGKEVQATVKVRSEGAEIAGLRLT
                     LRRNGEVQFSRDVRVDTPVRLPLKIDRAGINLFEAEIEVAPGELTPLNNLIALPVEGV
                     RDKLRVLLVSGEPHAGERTWRNLLKADPNVDLVHFTILRPPEKQDGTPTSELSLIAFP
                     TRELFQQKIQEFDLIIFDRYSNQTYLPNAYFDNMVRYVREGGQMLFAVGPELLHRSGL
                     LSTPLRQIIPADPGTAPLERQFRARVSKLGERHPVTRKLEGAQLVAGPQGSEPSWAPW
                     FRQIPARAADGHVVMTGAENNPLLVLKREQKGRVALFLSDQVWLWARGYEGGGPYLDL
                     LRRLVHWLMKEPDLEEEALRLDVRGRTLTVERRTIGEAPGDVDLTLPGGEQLKLTLSE
                     AEAGIWRATVALRRAGLYKAQNGTLVAYANAGPANPKEFMEVVSTTSRLAPIAEESGG
                     SMRRLDQGGQLVVPRLVDIRSGGRFGGSDFIGLKPTEASVVRGVSVLALAAGGLGLLL
                     MLVPLIGTWLREGRGRRAT"
     gene            complement(4495..4992)
                     /locus_tag="PLQ11_07025"
     CDS             complement(4495..4992)
                     /locus_tag="PLQ11_07025"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HRK24690.1"
                     /translation="MTDLKITLHAEQPGDAEAIERLNERAFGPGRFARTAERIREQAG
                     PRLDLSFVARVATLLVGSVRLTPIRIGDAAGLMLGPLAVEPAFRSRGIGRLLIERALK
                     EAQAKGLKLVLLVGDLSYYGKLGFQPVPMGRITMPGPVDPARLLLWSADDAARSEAHG
                     AVRGG"
     gene            complement(5006..5524)
                     /locus_tag="PLQ11_07030"
     CDS             complement(5006..5524)
                     /locus_tag="PLQ11_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRK24691.1"
                     /translation="MMDRGDRPERRWLSRIAHLVFRFVRPMTLGVRAVVLDDSGRVFL
                     VRHTYVPGWHLPGGGVEAGETLVQAIAKELSEEANIRLTGPVQLHGVFHNSHASVRDH
                     VAVYVVRDFVQDGPRQPDHEIAETGFFPLDAVPAATTRGTRARLNEVTNGLARSETWE
                     PDMTRLGPGVKA"
     gene            5602..6492
                     /locus_tag="PLQ11_07035"
     CDS             5602..6492
                     /locus_tag="PLQ11_07035"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRK24692.1"
                     /translation="MPFLLAHLSDPHVGPLPQPRLVDLIGKRLTGYWNWHSKRRLIHN
                     MAVLEAIVSDIRCRCPQHVALGGDLVNIGLGAEFDLASRLLAPLGPAADVSIVPGNHD
                     AYVGESLRLMQARFGGHMRSDQDPDGFPFLRRRGGIALVGVSTAIPTLPFLASGAIGM
                     GQLGSLDRMLAELKQTGEPVVILMHHAPHQGGSRLFRGLRDAASLEAIIARHGAALIL
                     HGHNHKRSLAWLDGPDGSKVPAVGVASASAVPGDADHRAAWHLYHIDSGGSRPSIRLE
                     VRGMEATGAPIRILDEVQLT"
     gene            6489..6974
                     /locus_tag="PLQ11_07040"
     CDS             6489..6974
                     /locus_tag="PLQ11_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HRK24693.1"
                     /translation="MTQPALPAATERVVAAARSLGLAIDARVHAAPTRTAEEAAAACG
                     CDVGQIVKSLIFRGATSNRPYLLLVSGRNRVDEKGVAAHLGEDLRRPDAADVRALTSF
                     AIGGIPPFGHAEPMETLVDPDLLTYPTVWAAAGTPNTVFSIDPAVLAKATGARIVPMA
                     G"
     gene            complement(6981..8027)
                     /locus_tag="PLQ11_07045"
     CDS             complement(6981..8027)
                     /locus_tag="PLQ11_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRK24694.1"
                     /translation="MTDASHIETDCLIVGAGPVGLFAVFELGLLDVRAHLVDILGKPG
                     GQCAELYPEKPIYDIPGFPVITGQELTDNLMKQIAPFHPTFHYGEMITVVRPVGTAEA
                     PLFEVETDIGTRFRAKTVMIAAGGGSFQPKKPPIERIETYEGESVFYAVRRMEAFRGR
                     RVLIVGGGDSALDWAINLEPVAGRVTLLHRRDDFRGAPHSVEKMRDLVAAGRMDLRIG
                     QVSELKGAAPRLSAVACRDLDGNSFEIEADAMLPFFGLTMKLGPVADWGLNLNENLIA
                     VDTEKFETNVPGIFAVGDINTYPGKLKLILSGFHEATLAAQKVIRYVNPAKRIVFQYT
                     TSSTSLQRKLGVKD"
     gene            8175..9056
                     /locus_tag="PLQ11_07050"
     CDS             8175..9056
                     /locus_tag="PLQ11_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HRK24695.1"
                     /translation="MNDPSADYLLRPSPDDPRLTVRVEGIDHTGAAIVTPVTVERPLT
                     LFLNAREIVTMMTIGDYPEYLALGYLLNQAILKPDDPVSTIDYDDDLGVVVVRTEAPT
                     NFEEKLKKKVLTSGCAQGTSFGDLMAAIDTVQLPPGEVRTSWLYALSHKINRTPSLYL
                     EAGAIHGCVLCEQDRPLIYMEDVGRHNAVDKIAGYMARHGISPAGKLFYTTGRLTSEM
                     VIKTVRMGIPVLASRSGFTASGVELARKVGLTLIGRMRGQRFAALSGLDRIVFDTDPA
                     AVAEEHARHHRKGAADD"
     gene            9049..9672
                     /gene="mobA"
                     /locus_tag="PLQ11_07055"
     CDS             9049..9672
                     /gene="mobA"
                     /locus_tag="PLQ11_07055"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HRK24696.1"
                     /translation="MTERRPMGVILAGGLSSRMGGGDKPLKMVGGRTILQRIIDRLAP
                     QCDRMILNANGEAARFAAFGLPVVADTIPDYAGPLAGILAGLDWAASHRPDVADIISL
                     PADCPFLPADLVVRLAEARTAAGVPLAAAASGGWTHPVIGLWPVAIRNRLRAALAAGE
                     RKIDRFTPGIGIAHANWPTEPFDPFFNANAPDDLAAAETLAARYPNA"
     gene            9727..9864
                     /locus_tag="PLQ11_07060"
     CDS             9727..9864
                     /locus_tag="PLQ11_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24697.1"
                     /translation="MKAIVAALVAMGIISAGSYLVLNTTFQAPAEKAYATSGARVTIG
                     H"
BASE COUNT         1672 a         3405 c         3208 g         1800 t
ORIGIN      
        1 cggatcgcaa gcgccagttt gcgctgatca gcgatttcct cgccaatcgc gacggcgagg
       61 cagtgtggat caccgatgcg gtcgccattg gcgagaccga cgaggcgaca cagggcttca
      121 tcgctgcgct tggttcgaag ctgcgcatca tcgcggcgac aaccagcccg ctgcgcctca
      181 ttcgcgatgc ggcgaacagc cgcgatgcca tggtggtgaa actccagcgg ctcggggcgc
      241 ttgaaggcgc cgccgccggt atcgtccgcg cgagcgattc acgcggccgc accctggggg
      301 aggcgaattt cgcctttgcc accggcaatg acaccgaggc gcggctcgcc gtgccgctgg
      361 aagtgcgcaa cgatattgcc cgcatcgaga tcgccgatgg acgcagcgcc ggcgcggtct
      421 ggctgcttga tgccggcgcg cagcggcgca aggtggcgat catcagcggc gagacggccg
      481 atacggccca gccgctggtg tccggaacct atttcgtcac caaggcgctt gccccttatg
      541 ccacgctgag cgagccgccc cgcggcggcg atgctttgac ccgtgcgctg gaggatcggc
      601 cggacgtcat cgtgctggtc gatatcgcca cggtcgccgg cccggctctc gccgacctcg
      661 agaaattcgt cgaggatggc ggcgtgttga tccgctttgc cggctccggc cttgttgcgg
      721 ggtccgatgc attgctgccg gtcaagctcc ggcgtggcgg tcgcgttctc ggcggcgcgc
      781 tgtcctggga caaaccgcag cggctggccg ccttcaccga caccggcgcc ttcgccggcc
      841 ttcccgttcc ggccgatgtc accatcgagc ggcaggttct ggctgaaccg gagcccacgc
      901 ttaacgaacg cacctgggcc gcgctggggg acggaacgcc actggtcacg gctggccggc
      961 gcggcaaggg actgatcgtg ctgtttcacg ttaccgccga taccggctgg tcgaatctgc
     1021 cgttgtccgg cgtctttgtc gacatgctgc gcaagatcct gccgctcgcg gcaagcactg
     1081 cgacccgctc cgattccggc ccggaacgcg ccaatccccg ccgaattctc gatggtttcg
     1141 gcatattcgg tccgcccccg ccttctgcca agccgctgcc gcgcagccat gccggcggcg
     1201 ccagccagga tcatccgccc gggttctacg gcccgcccga agccagcatt gcggtcaaca
     1261 cgctggcgcc cgagacccgc gttgccccgc tcgatcttgc tggtgcaacg gtgcgtccgc
     1321 ttgcggcccg tccgccgctt gatctgcggc tgcccctgct ggcgcttgcg gtggtgctgt
     1381 tcctgctgga tgctctggcg gtgctgtttc tgtccggact gctgcgccgc tctgcgcttg
     1441 cggccggcgc ggttgcgctg tgtcttgtgc tgcccggcgg gcctgcggtc tcgcccgcgc
     1501 aggcgcagac gccatcggcg caggacgtcg aagcctcgct caagccgcgg ctggcctata
     1561 tcgtcaccgg caaccgcgag gtcgacgaga cctcccgcct cggcctgacc gccctgaccc
     1621 gcacgatggc tgcccgcacg gctatggagc cgggcgcacc gatcggactt gatcctgcca
     1681 aggacgagct ggtgttctat tcgctgatct actggccgat ggcatccgat cagccgatgc
     1741 cgtccgaggc cgcgctcagg cgcattgacc agttcatgaa aaacggcggc acggtgattt
     1801 tcgacacccg cgatgccacc gccatcgcca gcgccgccgg caccacggcg gagacccgtc
     1861 gcctgcgcca gatgctcagc aatctcgaca ttccgcaatt ggagccgatt ccgcgcgatc
     1921 acgtcataac caagaccttc tacctgctcg acaagctggt tggacgctat gcccagggcg
     1981 agacctggat cgaggccatg ccgcgcggcc tcggcgacga caagcgcccc gcccgggctg
     2041 gtgatcgcgt ttcgccgatc atcatcacct ccaatgatct ggctgccgcc tgggccgtcg
     2101 atcgctccgg caatccacga tttccgatgg tgccgggcga gccgcgtcag cgcgagatgg
     2161 cgatgcgcgc cggcgtcaat ctggtgatgt atgcgatgac cggcaactac aaggccgacc
     2221 aggtgcatgt gccggccctg ctggagcggt tggggcagtg atatgagcaa ccacttcagc
     2281 ctgtcattcg accccttcct gccatggccg gtgctgatcg gtcttgcgct catcgccctg
     2341 ctgctggcgg gacttctgat ctggcacggc aaacgggggg gatggacacg ccttgcggct
     2401 tgcgcactgc tcattgccgc gctgacggat ccgcgttttg tcaacgagga gcgcgagggc
     2461 ctgccggagg tggtggcgct tctggtcgat cgttccggca gccagggcat tggcgagcgc
     2521 gccgcccaga ccgatgcggc gagcggcgaa ctggaacagc gcattgccgc ccggcccaat
     2581 acaaccttgc gcaagatcga ggtgttcgag cgcgattcgg acgtcaacgg cacgcatctg
     2641 ttcgagggcc tttctgcggc gctggccgat gttccgcccg agcggcttgc cggcatcgtg
     2701 gcaatcaccg atggggttgt ccacgatgtc cctgcctcgt tgcgggcact tgggttcggg
     2761 gcgccctttc atggtctgat caccggcaag gcgggtgaat tcgaccgccg catcgaaatc
     2821 gtcgaatcgc cgcgtttcgg cattgtcggc aaggaggtgc aggctaccgt caaggtccgt
     2881 tcggaagggg ccgagattgc cgggctgcgc ctcacgcttc ggcgcaatgg cgaagtgcag
     2941 ttcagccgcg atgtgcgcgt tgacacccct gtccgcctgc cgctcaagat cgaccgcgcc
     3001 ggcatcaacc tgttcgaggc cgagatcgag gttgccccgg gcgagctgac cccgctcaac
     3061 aacctgatcg ccctgccggt ggaaggggtg cgtgacaagc tgcgcgttct gcttgtctcc
     3121 ggcgagccgc acgccggcga gcgcacctgg cgcaatctgc tcaaggccga tcccaacgtt
     3181 gacctcgtgc atttcaccat cctgcggccg ccggaaaagc aggacggcac gccgacgtcc
     3241 gaactgtcgc tgatcgcctt tccgacccgg gaactgttcc agcagaagat tcaggagttc
     3301 gatctgatca tcttcgatcg ctattccaac cagacctacc ttcccaatgc ctatttcgac
     3361 aatatggtac gctatgtgcg cgagggcggg cagatgctgt tcgctgtcgg tccggagctg
     3421 ctgcatcgct cggggttgct gtcaacgcca ttgcgacaga tcatccccgc agaccccgga
     3481 acagcgccgc tggaacggca gtttcgcgcc cgagtgagca aacttggcga gcgccatccc
     3541 gtcacccgta agctggaagg tgcccagctg gtcgccggcc cgcaaggcag cgaaccgtcc
     3601 tgggcgccgt ggttccggca gattccggcc cgggcggccg atggccatgt cgtcatgacc
     3661 ggcgccgaga acaacccgct gctcgtgctc aagcgggagc agaagggccg cgtcgcactg
     3721 ttcctgtccg atcaggtgtg gctgtgggcg cgcggctatg aggggggcgg gccctatctc
     3781 gatctgctgc gccgtctcgt ccactggctg atgaaggaac cggacctgga ggaggaggcc
     3841 ttgcgtcttg acgtgcgcgg ccgcaccctg acggtcgagc gccgcaccat cggcgaggcg
     3901 cccggcgatg tcgacctgac gcttcccggc ggcgagcagt tgaaactgac gctgagcgag
     3961 gccgaggccg ggatttggcg cgcaaccgtc gcgctgcgcc gcgccggcct ctacaaggcg
     4021 cagaacggca cgcttgtcgc ctatgccaat gccggcccgg ccaatccgaa ggaattcatg
     4081 gaggtagttt cgaccacgag ccggcttgcg cccattgccg aggaaagcgg cggctcgatg
     4141 cggcggctcg atcagggcgg gcaactggtc gtgccgcgtc tggtcgacat ccgcagcggc
     4201 ggccgctttg gcggttccga tttcatcggc ctcaagccaa ccgaagcctc cgtggtgcgt
     4261 ggcgtcagcg ttctggcact ggcggcaggc ggattgggat tgctgctgat gctggtgccg
     4321 ctgatcggca cctggctgcg cgagggacgg ggccgccggg cgacgtgatc aagagcattt
     4381 tcaagcgaag tggcgcccgg ttcgcgtaaa gaaaatgcga aaaaacaaaa agatagagca
     4441 cttttacgtg aattggattt cacggaaagc gctctagtca gatcggcttc tgctctagcc
     4501 gccacgcacc gcgccatgtg cctcgctgcg ggcggcatca tcggccgacc acagcagcag
     4561 ccgcgccgga tcaaccggcc ccggcatggt gatgcgcccc atcggcaccg gctggaaacc
     4621 cagctttccg taataagaca gatcgccgac cagcagcacc agcttcagtc ctttggcctg
     4681 cgcctccttc agcgcccgct cgatcagcag gcggccgatc ccgcggctgc ggaatgccgg
     4741 ttccacagcc agcggtccca gcatcagccc ggcggcatca ccgatgcgga tcggcgtcaa
     4801 ccgcaccgaa ccgaccagca gggtggcaac ccgtgccacg aacgacaggt cgagccgcgg
     4861 ccctgcctgc tcgcggatcc gctcggccgt gcgcgcaaac cgacccggac cgaaggcccg
     4921 ttcgttcagc cgttcgatcg cctcggcatc gccgggctgt tcggcgtgca gcgtgatctt
     4981 gagatctgtc atggaaccgg cgctatcatg ccttgacgcc ggggccaagc ctggtcatgt
     5041 ccggctccca ggtctcggat cgcgcaaggc cattggtcac ctcgttgaga cgcgcccgcg
     5101 tgccgcgcgt ggtcgccgcc ggcacggcat caagcgggaa gaagcctgtc tcggcgattt
     5161 catgatccgg ctgacgcggg ccatcttgca cgaaatcgcg caccacatag accgccacat
     5221 gatcgcgcac cgaagcgtgg gaattgtgaa acaccccatg cagctgcacg ggcccggtca
     5281 gccggatatt ggcttcctcc gacaattcct tggcgatggc ttgcaccagc gtttcgcccg
     5341 cctcgacgcc gccgcccggc agatgccagc ccggcacata ggtgtgccgc accaggaaaa
     5401 cacgcccgga atcgtccagc accaccgccc gcacccccag cgtcatcggc cggacaaagc
     5461 ggaagaccag atgggcgatg cggcttagcc agcgccgttc cggcctgtcg ccacgatcca
     5521 tcatcggcgt ccgcccttga cgggagccgc acaacaggaa acatcgtgcc gcacgttcac
     5581 caaccgagcc ggcatcacgc cttgcctttc cttctcgccc acctgtccga tccgcatgtc
     5641 ggcccgttgc cgcagccgcg ccttgtcgac cttatcggca agcggctgac cggttactgg
     5701 aactggcaca gcaagcgccg cctgatccac aacatggcgg ttctggaggc aattgtcagc
     5761 gacattcgtt gccggtgtcc gcagcatgtg gccctggggg gcgatctcgt caacatcggc
     5821 cttggcgccg agtttgacct cgcctcgcgc ctgctggcgc cgctcggccc ggctgccgat
     5881 gtcagtatcg tgccgggcaa tcacgacgcc tatgtcggcg aatcgcttcg gctgatgcag
     5941 gcacgcttcg gcggccatat gcggtccgat caggacccgg atggctttcc ctttctcagg
     6001 cgacgcggcg gcatcgcgct ggtcggcgtc tcgaccgcca ttcccaccct gccgtttctg
     6061 gccagcggcg ccatcggcat gggccagctt ggcagcctcg accggatgct tgccgagctg
     6121 aagcagacag gagagccggt ggtgattctc atgcaccacg cgccgcatca gggcggttca
     6181 cgattgtttc gcgggctacg cgatgcggcc tctctcgagg cgatcattgc ccgccatggg
     6241 gccgcgctga tcctgcatgg ccacaaccac aagcgttcgc tggcctggct cgacgggccg
     6301 gacggcagca aggtgccggc agtgggcgtt gcctccgctt ccgccgttcc gggcgatgcg
     6361 gaccatcgcg ccgcctggca cctctaccac atcgacagcg gcggctcccg cccgtcgatc
     6421 cggcttgagg tgcgtggcat ggaggcgacc ggcgctccga tccgtatcct cgacgaggtg
     6481 caactgacat gacccagcct gccttgcccg ccgcgaccga gcgcgtcgtc gcggccgccc
     6541 gatctctcgg tctggccatc gacgcgcgtg tgcacgcagc acccacccgc acggccgagg
     6601 aagcggcagc ggcctgtggc tgcgatgtcg gccagatcgt caaatcgctg atttttcgcg
     6661 gcgcgacgag caatcggccc tatctgctgc tggtctccgg tcgcaaccgt gtcgatgaga
     6721 agggcgttgc cgcccatctc ggcgaagatt tgcgtcgtcc ggatgcggcg gatgtgcggg
     6781 cgctcaccag ctttgccatc ggcggcattc cgcccttcgg ccatgcggaa ccgatggaaa
     6841 cactggttga tcccgatctg ttgacctacc ccacggtctg ggcggccgcc ggcacgccca
     6901 acaccgtttt ttccatcgat cccgccgtcc tcgcaaaagc caccggagcc cgcattgtcc
     6961 cgatggcggg gtgagcgctg tcagtccttc acgccgagct tgcgctgcaa cgaagtcgac
     7021 gaggtcgtat actggaacac gatccgcttg gccgggttga cgtagcggat caccttctgc
     7081 gccgccagcg tcgcctcgtg gaagccggag aggatcagct tcagcttgcc cggataggtg
     7141 ttgatgtcgc cgaccgcgaa aatgccgggc acattggtct cgaacttctc ggtatcaact
     7201 gcgatcaggt tttcattgag gttcaaaccc cagtcggcga ccggtccaag cttcatggtg
     7261 aggccgaaga acggcagcat cgcatcggcc tcgatctcga agctgttgcc atcgaggtcg
     7321 cggcaggcaa ctgcggaaag gcgcggtgcg gcgcccttga gttcgctgac ctgaccgatg
     7381 cggaggtcca tccggccggc cgcgaccagg tcccgcatct tttccaccga atgcggcgca
     7441 ccgcggaaat cgtcgcgccg gtgcagcagc gtcacccgcc cggcgacggg ttcgagattg
     7501 atcgcccagt cgagtgccga atcgccgccg ccgacgatca ggacgcgacg cccgcggaac
     7561 gcctccatcc tgcgcacagc gtagaacacg ctttcgcctt cgtaggtctc gatccgctcg
     7621 atcggcggct tctttggctg gaatgagccg ccgccggccg cgatcatcac cgttttggcg
     7681 cggaaccggg ttccgatgtc ggtttcgacc tcgaacagcg gcgcctcggc ggtgccgacg
     7741 gggcgcacca cggtgatcat ctcgccataa tggaaggtcg gatgaaaggg cgcgatctgc
     7801 ttcatcagat tgtcggtcag ttcctgtccg gtgatcaccg gaaatcccgg aatgtcgtag
     7861 atcggcttct ccggatagag ttcggcgcat tggccgcccg gcttgccgag aatgtcaacg
     7921 agatgggcgc ggacatcgag cagccccagt tcgaacaccg cgaacaggcc caccgggccc
     7981 gccccgacaa taaggcaatc cgtttcgata tgactggcgt ccgtcatgca tctgtctcct
     8041 tggttgccgc gccccgcaga ccaaccgtca gggatgaggc tgttagtcgc tgatggcggc
     8101 aatcgcaagg atctcgatca aaccatcgca attgccgcat ctcttcccta tctgtgaatc
     8161 agcgaggtgc tgccgtgaac gacccttccg ccgactatct tctgcgtccc tccccggatg
     8221 atccgcgtct caccgtccgt gtcgagggga tcgaccacac gggcgcagcg attgtcacgc
     8281 cggtgaccgt ggagcggccg ctgacgctgt ttctcaacgc ccgcgagatc gtcacgatga
     8341 tgaccatcgg cgattacccc gagtatctgg cgctcggcta tctgctcaac caggcgatcc
     8401 tcaagcccga cgatccggtg agcaccatcg actatgatga cgatctcggc gtcgtcgtgg
     8461 tgcgcaccga ggcaccgacc aacttcgagg aaaagctgaa gaagaaagtg ctgacctccg
     8521 gttgcgcgca gggcaccagc ttcggcgatc tgatggcggc catcgacacg gtgcagcttc
     8581 cgcccggcga ggtgcgtaca tcctggctct acgcgctttc gcacaagatc aaccgaacgc
     8641 cctcgctcta tctcgaagcc ggggcgatcc atggctgcgt gctgtgcgag caggaccggc
     8701 cgctgatcta catggaggat gtcggccggc acaatgcggt ggacaagatc gccggctaca
     8761 tggcccgtca cggcatctcc ccggccggca agctgttcta caccaccgga aggctcacct
     8821 cggaaatggt catcaagacc gtgcggatgg gcattccggt gctggcctcg cgttccggct
     8881 tcaccgcctc gggcgtcgag cttgcccgca aggttggcct caccctgatc ggccgcatgc
     8941 gcggccagcg ctttgccgcc ctgtcggggc tcgaccgcat cgtgttcgat accgatccgg
     9001 ctgccgttgc cgaagaacat gcccgtcacc atcgcaaggg agccgccgat gactgagcgt
     9061 cgacctatgg gcgtcatcct cgccgggggc ctgtcaagtc ggatgggggg tggcgacaaa
     9121 ccgctgaaaa tggtcggtgg ccgcacgatc ctccagcgca tcatcgaccg tctggcaccg
     9181 caatgtgatc gcatgatcct caatgccaac ggcgaggcgg cgcgttttgc cgcttttgga
     9241 ctgccggtgg tggccgatac gatccccgac tatgccgggc cgctcgccgg cattctcgcc
     9301 ggtctggact gggcggccag ccatcgcccc gatgttgccg acatcatcag cctgccggcc
     9361 gattgcccgt ttctgcccgc cgatctggta gtgcgcctcg ccgaggcccg gacagccgcc
     9421 ggggtgccgt tggccgccgc cgcgtccggg ggctggaccc atccggtgat cggcctgtgg
     9481 ccggtcgcca tacgcaaccg cctgcgcgct gcgcttgcgg ccggcgagcg caagatcgac
     9541 cgcttcacgc caggcatcgg catcgcccat gccaactggc cgaccgaacc gttcgatccc
     9601 ttcttcaatg ccaatgcgcc cgacgatctt gccgctgccg aaacgcttgc agcccggtat
     9661 ccgaatgcgt aaaacatgat ccaacaacag gatgcagagc ggcatcggaa catacgggac
     9721 ggatcgatga aagccatcgt tgcagcactt gtcgccatgg gcattatcag cgcggggagc
     9781 tatctggtct tgaacacgac ctttcaggcc ccggccgaga aggcttatgc caccagcggc
     9841 gcgcgcgtca caataggcca ttgaggccat cagaccaacg cccccggaca tggctggcag
     9901 gtcataatgg ggaattgctg gttaggagcg aatgcgacgc cgagcagggt cttaaggccc
     9961 ggttgcgagg aatgctcgag ggatgaccga acagtcagaa ccgagggcca ctggcagaag
    10021 cccctgacat cagcgaggca taggcaagaa accgcaccgc cgtccccggc gtgacattgg
    10081 tggtt
//
LOCUS       DAOMXG010000083        10040 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_3554_length_10040_cov_8.985578, whole genome shotgun sequence.
ACCESSION   DAOMXG010000083 DAOMXG010000000
VERSION     DAOMXG010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 10040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10040
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3554_length_10040_cov_8.985578"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /gene="argS"
                     /locus_tag="PLQ11_07065"
     CDS             complement(<1..848)
                     /gene="argS"
                     /locus_tag="PLQ11_07065"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRK24698.1"
                     /translation="MHLFADFHARIARVLASLPATAALPDSVRGRVTVEPPRDPSHGD
                     LSTNAAMVIARDAGMKPRDLAESIAAALRAEPGVVAVEVAGPGFINIRLQPAIYDDVL
                     RAILAAGPAFSRTGLGGGAVVNVEYVSANPTGPMHVGHCRGAVYGDALANLLAATGYK
                     VTREYYINDAGAQVDVLARSAFLRYREALGEDIGTIPEGLYPGDYLKPVGVALVEAHG
                     ARLKTLPEAEWLPIVRKAAIDGMIAMIRDDLAALNIHHEVFFSERSLQGGGNGGAVGA
                     AIEALRA"
     gene            complement(1000..2208)
                     /locus_tag="PLQ11_07070"
     CDS             complement(1000..2208)
                     /locus_tag="PLQ11_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HRK24699.1"
                     /translation="MLFHRERLLSPFAVDAANSRGRLVPEPASPTRSEFQRDRDRIIH
                     STAFRRLKHKTQVFVEHEGDHFRTRLTHTIEVAQIARAIARALALDEDLAEALALAHD
                     LGHTPFGHVGEDVLQELMAAHGGFDHNAQALRIVTRLERRYAAYDGLNLTWETLEGLV
                     KHNGPLTDANGQPTERYRRHGVPGAILEFDARFPLDLASHASAEAQVAALADDIAYNA
                     HDIDDGLRAGLFALDDLMAVPWLAELIAEIDAAWPGLEPARRQHELVRRLITRLVEDV
                     IGESERRLVALAPRSAEDIRNAGRPVVAFSEGVATAEKDLKRFLFARMYRHPRVMVIR
                     TDAASIVEDLFGRFVEAPSLMPGEWHAGLDGADAARIARRVADYIAGMTDRYAIDAHR
                     RLFDETPALR"
     gene            2578..2916
                     /gene="erpA"
                     /locus_tag="PLQ11_07075"
     CDS             2578..2916
                     /gene="erpA"
                     /locus_tag="PLQ11_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HRK24700.1"
                     /translation="MPVPTSDVSVTPRAARRIAAILKDEVAGAMLRVSVKGGGCSGFQ
                     YAFEVEPRAATDDLSIVQDGVTVLVDPTSLPFLKGAAIDFVDDLMGASFRVNNPNATV
                     ACGCGTSFSI"
     gene            3082..5097
                     /locus_tag="PLQ11_07080"
     CDS             3082..5097
                     /locus_tag="PLQ11_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24701.1"
                     /translation="MTRCKHSLLISASLFALVTAGSLLADRIGAAAVREQLTGAGAEA
                     RGISVSTLTGRVTIEGVRFAATGGHGEIARIHYSTGGGLVAPAFAQSGGSYVIEGLKV
                     EQPLVSVSIPSIQIDGASIGKDEWLKLFTFDAGLPARLKDLNVAAARVPLIEIRGTLK
                     AGDTLAYSLNDNRIENVRAGKAERIVTGSGSYSSTSTGMKQEGVIKQWEMRGLDLAQT
                     ARVWFNTASDGEKPSPLYESYLLEGMTSTAQGEVVMNIDISKVTSGAVRMRPLKGRSL
                     AAIVTDLMAFAEKNKDKKNTDKSAADKALEAELGRNVLPALVEVMDSFEDDGTSGENM
                     RFVVGEKAKPAATVSIAKFSGSYGSSTVPSGFLLSDLAVKSGDYTAKLAQFGISGFSY
                     APTLRGMAEAMASGDSDFSRIDPRKLMPRFGSYTLKGFELEGPAPKSPKGVKPERFAL
                     KVGQFAMAARDEVKGIPTNISLVLDNLAMTLPETPSEDWMKSIKALGYSSVDVSAKIA
                     AAWNEARKEVAISDISFGGAQMGQIKLSGTLGNIGRDLFEADAAMAQVALMGITAKSA
                     SLKVDNAGMAEKLIAMQARQQGRKPDELRSELGMMAAMGIPALLGPSDEAKMIAGAIA
                     KFLAKPKGLSIDLAARNAMGIGMPDMVMASDPQKALGLVTVKASASD"
     gene            5204..5839
                     /locus_tag="PLQ11_07085"
     CDS             5204..5839
                     /locus_tag="PLQ11_07085"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015821301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HRK24702.1"
                     /translation="MTDWLPPWLRRRLNPRNLTRQHLDALVQRHGFSIGDHSYGAPKV
                     RFAEGGGGLTIGKYCSIADQVEIFLGGNHRSDFVTTYPFGSFRKDWPDAQDLPSPARG
                     RGDVIIGHDVWIGFGALILSGVRIGNGAVIGARSVVSRDVPAYTIVAGSPARVLRPRF
                     SPGFIEQLQEVAWWDLPDKSVGKLARVLQSDRIEVAIEAIRTERARLNLAG"
     gene            complement(5823..6707)
                     /locus_tag="PLQ11_07090"
     CDS             complement(5823..6707)
                     /locus_tag="PLQ11_07090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018043509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRK24703.1"
                     /translation="MSVTAIIVAHDSAGILPDCLAALARDGVPALVVDNASADDTVAV
                     AEAAGARVIRNRLNEGYGRANDKGVVAADSDFCLILNPDIVLDPGCVARLLQAAADYP
                     DAGLWAPSIIEDDGRFFFQNRSLLAESVVRGPVSGDARAVPPEGDCCAPFLSGACFLV
                     RRQVFLAAGGFDPNIFLFYEDDDLCRRLQDGGRPLVHVHDAVARHGRGQSAPPTPERR
                     YRSRWHYGWSRIYVARKYGLPTGAAWTLAMNLPKALGVVFTLNRMEIARYWGLVAGTI
                     AGLAGRSAIGREGLSRRD"
     gene            complement(6723..8495)
                     /gene="aspS"
                     /locus_tag="PLQ11_07095"
     CDS             complement(6723..8495)
                     /gene="aspS"
                     /locus_tag="PLQ11_07095"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HRK24704.1"
                     /translation="MHRYRTHTCGALRASDIGTVARLSGWCHRIRDHGGVLFIDLRDH
                     YGMTQIVVDPDSPAFNAAEKVRSEWVIRIDGKVRKRPEGTDNANLSTGTVEVFVTELE
                     VLGPAGELPLPVFGDLDYPEDTRLTYRFLDLRRERLHRNIMTRVAIIDSMRQRMKTAG
                     FNEFNTPILTASSPEGARDFLVPSRIHPGKFYALPQAPQQYKQLLMMAGFDRYFQIAP
                     CFRDEDPRADRLPGEFYQLDLEMSFVEQEDVFTTMEPVITGIFEEFANGKPVTQQWPR
                     IPYATAMAKYGSDKPDLRNPIEMQDVSEHFRGSGFKVFARLLEDPKNRVWAIPAPTGG
                     SRGFCDRMNSWAQGEGQPGLGYILFREGGEPGGPIANNIGAERAIAIREQLGLKEGDS
                     AFFVAGNPEKFAKFAGAARVKVGEDLNLTKKDQFALAWIVDFPMFEYNEDEKKVDFSH
                     NPFSMPQGGLEALNGQDPLTIKAFQYDIVCNGFEIASGGIRNHKPEAMVKAFEIAGYS
                     EATVVERFGGMYRAFQYGAPPHGGMAAGVDRIVMLLCGTQNLREISLFPMNQQALDLL
                     MGAPSDVTNKQLRELHLRLNLPEK"
     gene            complement(8572..9213)
                     /locus_tag="PLQ11_07100"
     CDS             complement(8572..9213)
                     /locus_tag="PLQ11_07100"
                     /inference="COORDINATES: protein motif:HMM:NF015423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HRK24705.1"
                     /translation="MPSEIVSASLIIVDYLAVIVFAISGALAAAEKKLDVIGCIWLAA
                     VTGIGGGTMRDLLLRQPVFWVHEPNYLALAGGAAILVYFLARIPKGSRTALLWMDAVG
                     LAFVSVAGAGKALDIGAPPSIAVLMGVITGVFGGIVRDMLVQEPSVLLKKELYITASL
                     AGALGYVGLFLLGSGQELAMAGGILSGFAIRAGSILYGWRLPNARLRAENSDE"
     gene            9312..>10040
                     /locus_tag="PLQ11_07105"
     CDS             9312..>10040
                     /locus_tag="PLQ11_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018097160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HRK24706.1"
                     /translation="MLPSVLIANRGEIACRIIRTARRLGMRSIAVYSDADARAPFVAM
                     ADEAHRIGPAPARESYLKGDVILDVARRSGAACVHPGYGFLSENTGFARACEAAGIVF
                     VGPPPEAIEAMGLKDAAKKIAEKAGVPVVPGYHGDNQDPGFLKQKAYEIGYPVLIKAV
                     AGGGGKGMRRVDKHADFDDALASCKREALNAFADDRVLIEKYVTAPRHVEIQVFADRH
                     GNVVHLFERDCSLQRRHQKVIEEAP"
BASE COUNT         1871 a         3205 c         3221 g         1743 t
ORIGIN      
        1 gcgcgcaggg cctcgatcgc cgcgccgaca gcgccgccgt tgccgccgcc ctgcaagctg
       61 cgctcggaaa agaaaacctc atggtggatg ttgagcgcgg caagatcgtc gcggatcatc
      121 gcaatcatgc cgtcaatcgc cgccttgcgg acaatcggca gccattcggc ttccggcagc
      181 gttttcagac gggcaccatg cgcctcgacc agcgcaacgc cgaccggctt gagataatcg
      241 cccggataca gcccctcggg gatggtgccg atatcctcgc ccagcgcctc gcggtagcgc
      301 aggaaggccg aacgggccag cacatcgacc tgcgcgccgg catcattgat gtaatattcg
      361 cgcgtcacct tgtagccggt cgccgccagc agattggcca gcgcatcacc atagaccgcg
      421 ccacggcaat ggccgacatg catcggcccg gtgggattgg ccgagacata ttcgacattg
      481 accaccgcgc cgccgccgag cccggtgcgg ctgaaggctg ggccggcagc gaggatcgcg
      541 cgcagcacat cgtcatagat cgccggctga agcctgatgt tgatgaagcc gggacccgcc
      601 acctcgaccg ccaccacgcc cggttcggcc cgcagggctg cggcgatgga ttcggccaga
      661 tcgcgcggct tcatgccggc gtccctggca atcaccatgg cggcattggt ggacagatcg
      721 ccatgcgagg gatcgcgcgg cggctcgacc gtcacgcgcc cgcgcaccga atccggcaat
      781 gcagcggtgg cggggaggct ggcgagcacc cgcgcgatgc gggcatgaaa atcggcaaac
      841 agatgcatgg gactggggtt ccggtcagtt cggagtgcgg tcgtcagatc acgctctcgt
      901 tttgatggaa atatcaaaac gagagataac gtgatcgtta tcaaatgctt agagcatgct
      961 tgttgcgatt agccggattc cacctaatcg cagcatgctc tagcgcaagg ccggcgtttc
     1021 gtcaaaaagc cttcggtgcg catcgatggc gtagcggtcg gtcatgccgg caatgtaatc
     1081 ggcgacccgg cgggctatgc gcgccgcatc cgccccgtcc agcccggcat gccattcacc
     1141 gggcatcagc gagggcgcct cgacaaaccg gccgaacaga tcctcgacga tgctggcggc
     1201 atcggtgcgg atcaccatga cgcgggggtg gcgatacatc cttgcgaaca gaaagcgctt
     1261 gagatccttc tcggcggtcg ccacgccctc cgagaaggcg accaccggcc ggccggcatt
     1321 gcgaatatct tccgccgagc gaggcgccag cgcgacaaga cgtcgttcgc tctcgccgat
     1381 cacgtcttcg accagccggg tgatcagccg gcgcaccaac tcatgctggc gccgtgccgg
     1441 ctccagcccc ggccaggctg catcgatttc cgcgatcagc tcggcaagcc atggcacagc
     1501 catgagatca tcgagcgcga acagcccggc gcgcaggcca tcgtcgatgt catgggcgtt
     1561 gtaggcgatg tcatcggcga gcgcggcgac ctgcgcctcg gcgctggcgt gggaggccag
     1621 atccagcgga aagcgggcat cgaattcaag gatggcgccc ggcacgccat ggcgacggta
     1681 gcgctcggtc ggctggccgt tggcgtccgt cagcgggcca ttgtgcttga ccagcccttc
     1741 cagcgtctcc caggtcaggt tgagaccgtc ataggcggca tagcggcgct ccagccgggt
     1801 gacgatgcgc aaagcctggg cattgtggtc aaagccgccg tgggcggcca tcagttcctg
     1861 caagacgtcc tcgccgacat ggccgaacgg cgtatggcca agatcgtggg caagcgctag
     1921 cgcctcagcc aggtcctcgt ccagcgccag ggcgcgcgca atcgcccggg caatctgggc
     1981 cacttcgatc gtatgcgtca gccgcgtgcg gaaatgatca ccttcgtgct cgacgaatac
     2041 ctgagtcttg tgcttgagcc gacggaaggc ggtcgaatgg atgatccggt cgcggtcgcg
     2101 ctggaattcg ctgcgggttg gcgaagccgg ctccggcacc agccggccgc ggctgtttgc
     2161 ggcatcgacg gcaaatggcg agagcaaacg ttcccggtga aacagcacta gagcactttc
     2221 cgatcagatt gaaccgattg cgtttcacgg gccgcgcggc gatccgggcg gaggctggcg
     2281 agaagcgtat cctgcgatac ggtcgagcca gcgtccaaac cgggcgcccg cggcccggaa
     2341 acccggaggg atgaccattt tcgggccaaa tccggcgaaa aatgctccaa ccgtatcaac
     2401 ggatacgctt gtcgcattgt ccttgatccg aaactggtca tcgcaatgat tcaatctggt
     2461 cggaaagggc tctagcaccc cgacagccgg agagggttga agcccgacca ttctgctctt
     2521 accttgtttg tgacgccgcc ggaatacggg ggcggagccg aagcgaaaag agaaccgatg
     2581 cctgttccga cctccgacgt ttccgtgacc ccgcgcgccg cgcggcgcat tgcggcaatc
     2641 ctcaaggacg aggttgcggg cgcgatgctg cgggtatcgg tcaagggcgg cggctgttcg
     2701 ggcttccaat atgctttcga ggtcgagcca cgggctgcga ccgatgatct cagcattgtc
     2761 caggacgggg tgacggtgct ggtcgatccg acctccctgc ccttcctcaa gggcgcggcg
     2821 atcgattttg tcgatgacct tatgggcgcc tcattccggg tcaacaatcc aaatgcgacg
     2881 gtcgcctgcg gctgcgggac gtcgttctcg atctgagccg gctgcgcgcg tcgcctgctc
     2941 caacctcctg ccgtgacgtg gcgggtcaac agtctgtaaa ccgcgcctac gccggggcca
     3001 tagccatggt tcaacgaata tggtgaggtt ccgcggttca attgatgctt ttttgagtcg
     3061 ccaaacccca gggagggttc catgacccgt tgcaaacact cgctgctcat ttccgccagc
     3121 ctgttcgcgc tcgtgaccgc cgggtcgttg cttgccgacc ggattggcgc cgctgccgtg
     3181 cgcgagcagc tgaccggcgc cggagccgag gcgcgaggga tcagcgtgtc aaccctgacc
     3241 ggccgggtga ccatcgaggg cgtgcgcttt gccgcgacgg gggggcacgg cgagattgcc
     3301 cgcatccatt attcgaccgg gggcgggctg gttgcgcctg cctttgccca gagcggcggc
     3361 agctatgtca tcgaaggtct caaggtcgag cagccgctgg tttcggtctc catcccgtca
     3421 atccagatcg acggcgcctc gatcggcaag gacgaatggc tgaagctgtt cacattcgat
     3481 gccggcctgc ccgcgcggct caaggatctg aatgtcgcag cagcgcgcgt gccgctgatc
     3541 gagatccggg gaacgctgaa agccggcgac acgctcgcct attcgctgaa tgacaaccgc
     3601 atcgaaaacg tccgcgccgg caaggcggaa aggatcgtca ccggctcggg gtcgtattcg
     3661 tcgacctcga ccggcatgaa gcaggagggc gtcatcaagc aatgggagat gcgcggcctc
     3721 gatctcgccc agacagcccg cgtctggttc aacaccgcca gcgatggcga aaagccctcg
     3781 ccgctctatg agtcctatct tctcgaaggc atgacctcga ccgcccaggg cgaagtcgtg
     3841 atgaacatcg acatcagcaa ggtcacgtcc ggtgcggtgc gcatgcgtcc gctcaagggt
     3901 cgcagtctgg cagcgatcgt gaccgatctg atggcctttg ccgaaaagaa caaggacaag
     3961 aagaataccg acaagtcggc agccgacaag gcgctggagg ccgaactggg gcgcaatgtg
     4021 ctgccggctc tggtcgaggt tatggattcg ttcgaggatg acggcacatc cggcgagaac
     4081 atgcggttcg ttgttggcga gaaggccaag cccgccgcca cagtcagcat cgccaagttc
     4141 tccggttcct atggcagcag caccgtgccc tcgggtttcc tgctcagtga tcttgcggtc
     4201 aagagcggcg actacacggc aaagctggcg cagttcggca tctcggggtt ttcctatgcg
     4261 ccgaccttgc gcggcatggc cgaggcgatg gccagcggcg acagcgattt ctccaggatc
     4321 gacccgcgca agctgatgcc gcggttcggc agctacaccc tcaaggggtt tgaactggag
     4381 gggccggcgc ccaaatcgcc caagggcgtc aagccggagc ggtttgccct caaggtcggc
     4441 cagttcgcaa tggcggcgcg ggacgaagtg aaaggcattc ccaccaacat ttcgctggtt
     4501 ctcgacaatc tggccatgac cttgccggaa acaccgtccg aggactggat gaaatccatc
     4561 aaggcgcttg gctattcctc ggttgatgtt tcggccaaga tcgcggccgc atggaacgag
     4621 gccaggaagg aagtggcgat ttccgatatc tccttcggcg gggcgcagat gggccagatc
     4681 aagctcagcg gaacgctggg caatatcggc cgcgacctgt tcgaggccga tgcggccatg
     4741 gcgcaggtgg cactgatggg cattaccgcc aaatccgcca gcctgaaggt cgacaatgcc
     4801 ggcatggcag aaaagctcat tgccatgcag gcacgccagc agggccgcaa gcccgacgag
     4861 ctgcgctccg aacttggcat gatggcggcg atgggcattc ctgccctgct cggcccctcg
     4921 gatgaggcca agatgatcgc cggggccatt gcgaagttcc tggccaagcc gaaggggctc
     4981 agcatcgatc ttgccgcccg caatgcgatg gggatcggca tgccggatat ggtgatggcc
     5041 tccgatccgc agaaggcgct cggtctggtc acggtgaagg ccagcgccag cgactgagcg
     5101 gcgccggctt gaagcggcct ttggtctttg tgcgggagtt catttcccgc acgggatgat
     5161 ataggcttgg cgcaatgttt ggcgcattcc tgagtatctg tccgtgaccg actggctgcc
     5221 gccctggctt cgccgccgcc tcaatccccg caacctgacc cgtcagcacc tcgatgcgct
     5281 ggtgcagcgg catggctttt caatcggtga tcacagctat ggcgcgccga aggtgcgctt
     5341 tgccgaaggc ggcggcggcc tgacaatcgg caaatattgt tcgatcgccg atcaggtgga
     5401 gatcttcctc ggtggcaatc accgaagcga tttcgtcaca acctatccgt tcggctcgtt
     5461 caggaaggac tggccggatg cgcaggatct gcccagcccg gcacgagggc gcggcgatgt
     5521 gatcatcggc catgacgtct ggatcgggtt cggcgcgctg atcctgtcgg gagtccggat
     5581 cggcaacggg gcggtgatcg gcgcgcggtc ggtcgtctcg cgcgatgttc ctgcctatac
     5641 gatcgttgcc ggcagcccgg cgcgcgtgct ccggccgcgc ttctcgcccg gctttatcga
     5701 acaattgcag gaagtcgcct ggtgggacct gcccgacaaa agcgtcggca aactggcccg
     5761 cgtgctgcaa tcggatcgga tcgaggtcgc catcgaggcg atccgcaccg aacgggcgcg
     5821 gctcaatctc gccggctgag cccctcccgc ccgatggccg agcgtccggc cagcccggcg
     5881 atcgtgccgg cgaccagacc ccagtagcgg gcgatttcca tgcggttgag ggtaaacacc
     5941 acccccagtg ccttgggcag gttcatcgcc agggtccaag cggcgccggt cggcaggccg
     6001 tatttgcggg cgacatagat gcgagaccag ccgtaatgcc agcgtgagcg atagcggcgt
     6061 tccggcgtcg gcggcgctga ctggccgcgt ccatgccgcg ccaccgcatc atgcacatgc
     6121 acgagcggcc gcccgccatc ctgcaagcgg cgacagaggt cgtcgtcttc gtagaacagg
     6181 aagatattgg gatcgaaccc gccggccgcg agaaacacct gccgccgcac cagaaagcag
     6241 gcgcccgaca ggaacggagc acagcaatcg ccctccggcg gcaccgcccg cgcatcgccc
     6301 gaaaccggac cgcgcaccac cgattcggcc agcagagagc gattctggaa gaaaaaacgc
     6361 ccgtcatcct cgatgatcga cggtgcccac agcccggcat cgggataatc ggccgctgcc
     6421 tgcaagagcc gcgcgacaca gcccggatca agcacgatgt ccgggttgag gatcaggcag
     6481 aagtcactgt ccgccgcgac aacacccttg tcgttggccc ggccgtagcc ctcgttcagc
     6541 cgattgcgga tgacgcgggc tcccgccgct tccgccactg ccaccgtatc gtcggcactg
     6601 gcattatcga ccaccagagc cggcacaccg tcgcgcgcca gcgccgccag acagtccggc
     6661 aggatgccgg cgctgtcatg ggcaacgatg atggcggtga cgctcatggt gcgcgccccc
     6721 gctcacttct ccggcaaatt cagccgcagg tgcagttcgc gcagctgctt gttggtcacg
     6781 tcgctcggtg cgcccatcaa gaggtcgagc gcctgctggt tcatcggaaa cagcgagatt
     6841 tccctgaggt tctgcgtgcc gcagagcagc atgacgatgc gatcgacgcc tgccgccatg
     6901 ccgccatgcg gcggtgcgcc atactggaag gcgcgataca tgccgccgaa gcgctcgacc
     6961 accgtcgctt ccgaataacc ggcgatctcg aaggctttga ccatcgcctc gggcttgtgg
     7021 ttgcggatgc cgccgctggc gatctcgaag ccattgcaga cgatgtcata ctggaacgcc
     7081 ttgatggtca gcgggtcctg cccgttgagc gcctcaagcc cgccctgcgg catcgagaac
     7141 gggttgtgcg agaaatcgac ctttttctcg tcctcgttgt attcgaacat cgggaaatcg
     7201 acgatccagg ccagcgcgaa ctgatccttt ttcgtcaggt tcaaatcctc tccgaccttg
     7261 acgcgggcgg cgcctgcgaa cttcgcaaac ttttccgggt tgccggcgac gaagaaggcg
     7321 ctgtcgccct ccttgaggcc gagctgctcg cggatcgcga tggcccgttc ggcgccgatg
     7381 ttgttggcga tcgggccgcc cggctcgccg ccctcacgga acaggatata ccccagccca
     7441 ggctggcctt cgccctgcgc ccaggagttc atccggtcgc agaaaccgcg cgaaccgccg
     7501 gtcggcgcgg gaatggccca gacccgattc ttcgggtctt cgagcaggcg cgcgaacacc
     7561 ttgaagcccg aaccgcggaa atgctcggag acatcctgca tctcgatggg gttgcgcagg
     7621 tccggcttgt cggagccgta tttggccatc gctgtcgcgt aagggatgcg cggccattgc
     7681 tgcgtcaccg gctttccgtt cgcaaattcc tcgaaaatgc cggtgatgac cggctccatc
     7741 gtcgtgaaaa catcctcctg ctcgacaaag ctcatttcaa ggtcaagctg gtagaattcg
     7801 cccggcagac gatcggcgcg cgggtcctcg tcgcggaaac acggcgcgat ctggaagtag
     7861 cggtcgaaac ccgccatcat caagagctgc ttatactgct gcggcgcctg cggcagcgca
     7921 tagaacttgc ccggatggat gcgggagggc accaggaagt cgcgcgcccc ttccggcgac
     7981 gacgccgtga ggatcggcgt gttgaactcg ttgaagccgg cagttttcat gcgctggcgc
     8041 atcgaatcga tgatcgccac gcgcgtcatg atgttccggt gcagccgctc gcgcctcaga
     8101 tcgaggaagc ggtaggtcag ccgcgtatcc tcgggataat cgagatcgcc gaacaccggc
     8161 agcggcaatt cgccggcagg gcccagcacc tcaagctcgg tgacgaacac ttccacggtg
     8221 ccggtggaaa gattggcgtt gtcggtgcct tccgggcgct tgcgcacctt gccgtcgatg
     8281 cggatcaccc attccgagcg caccttctct gcggcgttga aggcagggct gtccggatcg
     8341 acgacgatct gcgtcatgcc gtaatggtcg cgcaggtcga tgaacagcac gccgccatgg
     8401 tcgcggatgc ggtggcacca gccggacagg cgggcgacgg tgccgatgtc ggaggcgcgc
     8461 agggccccgc aggtatgggt gcgataacga tgcatggcaa acctgtgatc tttccggctc
     8521 gccatgcgat ggagcgggcg gggtgtcaag ggcgcgaccg gccggaaggg gttattcgtc
     8581 gctgttctca gcccgcaacc gcgcattcgg cagacgccaa ccgtagagga tcgacccggc
     8641 gcggatagca aatccagaaa gaatgccgcc ggccatcgcc agttcctggc cgctgccgag
     8701 cagaaacaac ccgacatagc cgagcgcgcc ggccagtgat gcggtgatat acaattcctt
     8761 cttcaaaaga accgagggtt cctgcaccag catgtcgcgg acaatgccgc cgaaaacacc
     8821 ggtgatgact cccatcagga cagcgatgga cggcggggct ccgatgtcga gcgccttacc
     8881 tgcgccggcg acagaaacga aggccagccc caccgcatcc atccacaaca gggcggttcg
     8941 gctgcccttc ggaatccgcg ccagaaaata gacgagaatc gcagcgccgc cggccagcgc
     9001 gaggtagttc ggttcatgca cccagaacac cggctggcgc agcagcaggt cgcgcatcgt
     9061 cccgccgccg atcccggtga ctgccgccag ccagatgcag ccgatcacat cgagcttctt
     9121 ctccgcagca gccagcgcac cggaaatggc aaagacgatg acggcgagat aatcgacgat
     9181 gatgagactg gcggagacaa tttcactggg cacggtcttt cccctttgtg agttccggtc
     9241 tgacctactt ccctcgccgg ctcaaccggc taaagtggca acacaacact gagcgagtac
     9301 ccatgcccaa attgctccca tccgttctga tcgccaaccg cggcgaaatc gcctgccgca
     9361 tcatccgcac cgcgcggcgc ctcggcatgc gctccatcgc ggtctattcg gatgccgatg
     9421 ccagggcgcc gttcgtggcc atggccgacg aagcccaccg catcggcccg gctccagcgc
     9481 gggaaagtta tctcaagggt gatgtgatcc tcgacgtggc ccggcgctcc ggcgcggcct
     9541 gcgttcatcc gggctatggg ttcctgtcgg aaaataccgg ctttgcccgc gcctgcgagg
     9601 cggcaggaat cgtgttcgtc ggcccgccgc ccgaggcgat cgaggcgatg ggcctcaagg
     9661 atgccgccaa gaagatcgcc gagaaggccg gcgtgccggt cgtccccggc tatcatggcg
     9721 acaatcagga tccaggtttt ctcaaacaga aagcctacga gatcggctat cccgtactga
     9781 tcaaggcagt ggccggcggc ggcggcaagg gcatgcgccg cgtcgacaag cacgccgatt
     9841 tcgacgatgc cctcgcctcc tgcaagcgcg aggcgctgaa cgcttttgct gatgatcgcg
     9901 tgctgatcga gaaatacgtc acggcgccgc gccatgtcga aatccaggtg tttgccgatc
     9961 gccatggcaa tgtcgtgcat ctgttcgagc gcgattgttc gctgcagcgc cggcatcaga
    10021 aggtgatcga ggaggcgccc
//
LOCUS       DAOMXG010000084        10036 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_3555_length_10036_cov_8.914137, whole genome shotgun sequence.
ACCESSION   DAOMXG010000084 DAOMXG010000000
VERSION     DAOMXG010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 10036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10036
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3555_length_10036_cov_8.914137"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="PLQ11_07110"
     CDS             complement(<1..916)
                     /locus_tag="PLQ11_07110"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRK24707.1"
                     /translation="MALSAGAVTRLIDELRETIALEGPMSVARYMSLCLAHPRHGYYI
                     TRDPLGHSGDFTTAPEVSQMFGELVGLWAAGVWDGFGRPERIRLVELGPGRGTLMADA
                     MRAARALPAFRKALSIHLVEISPTLRQRQQERLASLGIPVVWHDRIEDVPAGGAEIIL
                     ANEFFDALPIHQFIVKGGVWRERLVGLGEAGQLQLGVSETPASVPFAAAAEGDVRELC
                     PAGLDVVASLARRWQAGPGALLAIDYGYARPRSGDSFQALKGHRHADVLADPGEADLT
                     AHVDFSALAQAARAAGLMVAPLLTQATLL"
     gene            complement(892..1689)
                     /gene="lgt"
                     /locus_tag="PLQ11_07115"
     CDS             complement(892..1689)
                     /gene="lgt"
                     /locus_tag="PLQ11_07115"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRK24708.1"
                     /translation="MLAIPFPMIDPIAVSIGPLVIRWYALAYIGGLLVGWWYARRLVR
                     QGALWGTVTRPSEVALDDLIVYMALGVVIGGRLGEVVFYNLPYYLAHPAEILQVWRGG
                     MSFHGGLIGAAVAAVLFARKAGVLTLTVFDLCSTVVPVGLLLGRVANFINAELWGRIS
                     DVPWAVIFPNAGPEPRHPSQLYQAFLEGAVLLVVLGLAAQRLGFAKPGRLAGIFGMGY
                     GIARLIGEFFREPDDHIGFLFGGWLTMGMALSVPVFAVGLWLYRRAR"
     gene            1810..2061
                     /locus_tag="PLQ11_07120"
     CDS             1810..2061
                     /locus_tag="PLQ11_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HRK24709.1"
                     /translation="MTSNPLLDNLSRMMTGAMGVAEGMKKEADGLVRAQFERMLRDMD
                     VVTREEFEAVKEMAARARDDNDALTARIEALEALLAKKK"
     gene            2323..2820
                     /locus_tag="PLQ11_07125"
     CDS             2323..2820
                     /locus_tag="PLQ11_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HRK24710.1"
                     /translation="MLNELDSGHADHPVDIVERLAAHNQWSFERSGDDEIAIEVTGTW
                     CSYNTSFTWVEEMESLHLACAFGLKVPEARRIEMLKLIAMINEQLWIGHFDLWPSEDV
                     VMFRHSLLLAGCSDALPRQCEAILDIALRTAERYYQAFQFVVWAGKRAEEALAGTLFE
                     TVGRA"
     gene            2817..3635
                     /gene="proC"
                     /locus_tag="PLQ11_07130"
     CDS             2817..3635
                     /gene="proC"
                     /locus_tag="PLQ11_07130"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009494598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HRK24711.1"
                     /translation="MNGRPALVLVGAGKMGGAMLEGWLAGGMAGDSIVVLDPLPGAAL
                     SDLARRSGIRINPPAADLASPDILVLAVKPQMLDSAAATLAALAGPDTLVVSIIAGKT
                     VADLAARMPRARAFVRAMPNTPAAVGRGIAGCAASPAVTTEQRRQADRLLAAVGSVVW
                     LDSEAQIDAVTAVSGSGPAYVFHLVEAMTEAGIALGLAPETAAQLARATIEGAGELLY
                     RDRDTSPAQLRVNVTSPGGTTAAALAVLMAPDGMTALMKRAIAAAHRRAGELSG"
     gene            3694..4398
                     /locus_tag="PLQ11_07135"
     CDS             3694..4398
                     /locus_tag="PLQ11_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRK24712.1"
                     /translation="MAKKSAKSSAKSAPAGRPADPRRIVVETMMTLAASQPYRSITLG
                     EIAGEAGISLADLRDLFPSKGAILGGLMRMVDREVLGGTAADMADETAHDRVLDVMLR
                     RFDALAPYRDGLRSVWKDLSTSPADLIAFNQAAVNSWRYMLESAAIDTNGHLGALRTQ
                     GAVLVFARAFATWLDDEPDMARTMAVLDRELKRGASVLGAADALHRLSAPFRGLMRAA
                     CDGRRARSAPAAAPEA"
     gene            4399..4740
                     /locus_tag="PLQ11_07140"
     CDS             4399..4740
                     /locus_tag="PLQ11_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020179264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HRK24713.1"
                     /translation="MTETISFVDFERVDIRVGTIVAVDPFPEARKPAYKLRIDFGPAL
                     GIRKSSAQITAHYAPETLIGRQVAAVVNFPPRQIGKFMSEVLTLGFPDESGAVVLISP
                     TLPVPDGGKLF"
     gene            complement(4746..6069)
                     /locus_tag="PLQ11_07145"
                     /pseudo
     CDS             complement(4746..6069)
                     /locus_tag="PLQ11_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009494602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(6118..6837)
                     /locus_tag="PLQ11_07150"
     CDS             complement(6118..6837)
                     /locus_tag="PLQ11_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009494603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRK24714.1"
                     /translation="MVRLPVPLLDDAPHVLVVDDDSRIRSLLSKFLKSNGYRVTTAGN
                     AAEAREHLNGLAFDLLVLDVMMPGESGFDLARAIRKTSSVPILMLTALTDLDDRLKGL
                     ELGADDYLGKPFDPRELLLRVNSILRRVAAVPPAAAPVGPEMVRFGPFQFHLGRGELK
                     HGEDTVRLTNRERDILRQFAQALGSTVARELLADPDSGANERTIDVQINRLRRKIEPD
                     AANPTYLQTVRGVGYRLVVDP"
     gene            complement(6837..7379)
                     /locus_tag="PLQ11_07155"
     CDS             complement(6837..7379)
                     /locus_tag="PLQ11_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018045103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRK24715.1"
                     /translation="MNDRPAAAMRDSGVTAVSAPEGRGTLDPVALVELIFFAYRDFIG
                     EPDRVLERYGFGRAHHRVLHFVNRNPGLTVAELLSILDITKQSLGRVLRDLVAGGFIV
                     QKAGQQDRRQRLMYLSATGTALAEELLGLQTRRMGRALERLSEAEVAAVTGFLGGLID
                     ADQRSEVARLTRLTLDQRRR"
     gene            7554..8432
                     /locus_tag="PLQ11_07160"
     CDS             7554..8432
                     /locus_tag="PLQ11_07160"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HRK24716.1"
                     /translation="MSILPFDDRDGFIWFNGKLIPWREAKLHVLSHGLHYGGAVFEGE
                     RAYAGKIYKSREHSERFLKSAELLDFTIPYSPAELCAAKQAVIDANGGGDLYVRPVAW
                     RGSEMMAVAAQNATIHVAIAVWAWPSMFDINTKMKGIRLDIAEFRRPDPATAPCASKA
                     AGLYMICTISKHRAERKGYADAMMLDWQGRVAECTGANIFFTRDGAIHTPIADCFLNG
                     ITRQTVIDIARREGFEVIERRIMPEELAGFNECFITGTAAEVTPVSEIGPYKFAPGNI
                     SRSLIEAYTREVTPTA"
     gene            complement(8451..>10036)
                     /locus_tag="PLQ11_07165"
     CDS             complement(8451..>10036)
                     /locus_tag="PLQ11_07165"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRK24717.1"
                     /translation="RLEKLIGLAEVKRELASLKALMLANQRRRAQGLPVAPVSSHLVF
                     TGNPGTGKTTVARLIGEIYRELGVCASGHVVEVDRSSLVEGYVGGTEKKTREAIEKAL
                     DGVLFIDEAYTLAKQGSSSDTGVEAINTLLKAMEDHRDRLTVIVAGYRNEMRAFIDAN
                     PGLKSRFTRMIHFEDYTAEELMEIFEALVRQQKLEIGEDAHERIAFVLKEMHRTRDEQ
                     FGNAREVRAFFEKMIERQAQRLFAHPQADPSKFRAADIPDQGFGQKLDLDHVMDKLDR
                     LTGLGGVKAEVKKLINVARVNERRVAQSMAPLPMSLHLVFTGNPGTGKTTVARLVGEI
                     YAALGLVRRGHVVETDRAGLVAGYVGQTAAKTKDKIKEALDGVLFVDEAYTLNGAGSN
                     DFGQEAIDTLLKEMEDKRERLAVIIAGYTDEIAGFIDSNPGLKSRFSRTITFEDYTPE
                     EMMDIFSRLAVAQGYSLDEAAREALLARFSDMYATRAKDFGNGRSVRQLFEQVLERQA
                     GRVVDDPDCDVAAITAADV"
BASE COUNT         1809 a         3353 c         3117 g         1757 t
ORIGIN      
        1 ccagcaaggt cgcctgcgtc agcagcggcg cgaccatcag ccccgccgcc cgcgcggcct
       61 gcgccagagc cgagaaatcg acatgcgcag tcaggtctgc ctcgcccgga tcagccagca
      121 catcggcatg acggtgcccc ttcaacgcct ggaaactgtc gccggaacgg ggcctcgcat
      181 agccgtaatc gatcgccagc aaggcccccg gccctgcctg ccaacgccgt gccagcgaag
      241 cgaccacatc aagcccggcc gggcaaagct cgcgaacatc gccttcggct gcggcggcga
      301 acggcaccga agccggtgtt tccgagacgc caagctgcaa ctgccctgcc tcgccaagcc
      361 cgaccagccg ctcacgccag accccgccct tgacgatgaa ctggtggatc ggcagggcat
      421 cgaaaaactc attggcgagg atgatctcgg ccccgcccgc aggcacatcc tcgatcctgt
      481 cgtgccagac aaccggaatg ccgaggcttg ccagccgctc ctgctggcgc tggcgcagcg
      541 tcgggctgat ctcgaccaga tggatgctga gcgccttccg gaacgccggc aatgcccgcg
      601 ctgcgcgcat cgcatcggcc atcagcgtgc cgcgcccggg gccaagttca accaggcgga
      661 tccgttccgg ccggccgaac ccgtcccaca cccctgcggc ccacaggccg acaagttcgc
      721 cgaacatctg gctgacttcc ggagcggtgg tgaaatcccc cgaatgcccc agcggatcgc
      781 gcgtgatgta atagccgtgg cgcggatggg cgaggcataa actcatgtag cgggcaacgg
      841 acatcggccc ttcgagggca atcgtctcgc gcaattcgtc gatcagccgc gtcaccgcgc
      901 ccgccgatag agccacagcc cgaccgcgaa caccggcacc gacagcgcca tgcccatcgt
      961 cagccagccg ccgaacagga agccgatatg gtcgtccggc tcacggaaga attcgccgat
     1021 caagcgggcg atgccatagc ccatgccgaa aatgccggcg agccgtccgg gtttggcaaa
     1081 ccccagacgt tgcgccgcca gcccgagcac caccagcagc accgcccctt ccagaaacgc
     1141 ctgatagagc tggctcggat ggcgcggctc cggtcctgcg ttcggaaaga tcaccgccca
     1201 gggcacatcg ctgatccgcc cccacagttc ggcattgatg aaattggcaa cgcgccccag
     1261 cagcagcccc accggcacca cggtcgagca caggtcgaat acggtcagtg tcaaaacccc
     1321 ggccttgcgc gcaaacagga ccgccgccac ggccgcgccg atcagcccgc cgtggaacga
     1381 catgccgccg cgccacacct gcaggatctc cgccggatgg gcgagataat aaggcaggtt
     1441 atagaacacg acctcgccga gccgcccgcc gatcaccacg cccagcgcca tgtagacgat
     1501 gagatcgtcc agcgcgacct cgctgggccg ggtcactgtc ccccacaacg cgccctggcg
     1561 caccaggcgc cgcgcatacc accagccgac cagcagcccg ccgatatagg caagcgcata
     1621 ccagcggatg accaggggac cgatcgacac cgcgatcgga tcgatcattg gaaagggaat
     1681 cgccagcatg cccatgatgc tgcgaggatt tgcgccggcg catgcctgcg gtcaatggtc
     1741 aaatcggctg gccatgctta tatggaagcg agcgcccgcc ggggcaccat tcgccaggac
     1801 ctttccgcca tgacctccaa tccgctgctt gacaatctct cccgcatgat gaccggcgcc
     1861 atgggcgtcg ccgagggcat gaaaaaggag gccgatggcc tcgtccgcgc ccagttcgag
     1921 cgcatgttgc gtgacatgga tgtcgtcacc cgcgaggaat tcgaggcggt caaggaaatg
     1981 gcggcgcgcg cccgcgatga caatgacgcg ctgacggccc gcatcgaggc gctcgaagcg
     2041 ctgctggcca agaaaaaata gccgcagccc gaatctgtgg aatttgaaaa atcacgtgtt
     2101 gtcggaacgg cgcaaatcac cgattcttca atgcgtcggc tgctgatgag tgcgcgcggc
     2161 gacagtgtga acgtgtagcc aggccggttt gccggtcgcg tcgcggatcg gtttttcctt
     2221 catccgcttt gttgaggtgt gttggtccca gcaacacagc cgtggcttgt cgtttgcctg
     2281 ttgtctcgcc tcgggggcgc cgagcgcaga tgaggatcga cagtgctgaa tgaactcgac
     2341 agcggccacg cggaccaccc cgttgacatc gtcgaacggc tggctgctca caatcaatgg
     2401 tctttcgagc gctccggcga cgacgagatc gccatcgagg tgactggcac ctggtgcagc
     2461 tacaacacca gtttcacctg ggtggaggag atggaatcgc tgcatctggc ctgcgccttc
     2521 gggctgaagg tgccggaggc gcggcggatc gagatgctca agctgattgc catgatcaac
     2581 gagcaattgt ggatcggtca tttcgatctc tggccgtcag aggatgtggt gatgttccgc
     2641 cattcgctgc tgcttgccgg ctgttccgac gccctgccac ggcaatgcga ggccattctg
     2701 gatatcgcgt tgcgcaccgc cgaacgctac tatcaggcgt tccagttcgt tgtctgggcc
     2761 ggcaagcgcg ccgaggaggc gctggccgga accctgtttg aaaccgtggg gcgggcatga
     2821 acgggcgccc cgcccttgtt ctggtcggtg ctggcaagat gggcggcgcc atgctggaag
     2881 gctggctggc cggcggcatg gccggtgaca gcatcgtcgt gctcgatcca ttgcccggcg
     2941 cggccttgag cgatctggcc aggcgcagcg gcatccggat caatccgcct gctgccgacc
     3001 tcgcatcgcc tgacattctg gtgctggcgg tcaagccgca gatgcttgac agcgccgccg
     3061 cgacgcttgc cgccctcgcc ggtccggaca cgctggtggt ctcgatcatc gccggcaaga
     3121 ccgtcgccga tcttgcggcg cgcatgccgc gcgcgcgcgc tttcgttcgc gccatgccca
     3181 atacccccgc tgccgtcggc cgaggcattg ccggctgcgc cgcctcgccg gcggtcacaa
     3241 cagaacagcg caggcaggcc gaccggctgc ttgcggcagt cggatcagtg gtctggctgg
     3301 acagcgaggc gcagattgac gcggtgaccg ccgtctccgg ttctggccct gcctatgtgt
     3361 ttcatctggt cgaagcgatg accgaggcag ggatcgcgct cggcctggca ccggagacag
     3421 cggcgcagct ggcacgggcc acgatcgaag gcgccggcga actgctttat cgggacaggg
     3481 acaccagccc tgcccagctg cgcgtcaacg tgacctcgcc gggcggcacc accgccgccg
     3541 cgctcgccgt tctgatggcg cccgatggca tgaccgcgct gatgaagcgc gcgattgccg
     3601 ctgcccaccg ccgcgccgga gagctttcgg gctgaaccct gccttgctgg cgcctgattt
     3661 acggttagga tgaggcagcg acaggagaga gacatggcaa agaagtccgc caaatcctcg
     3721 gccaaatccg cgcccgccgg gcgccctgcc gatccgcgca ggatcgttgt cgaaaccatg
     3781 atgacgcttg ccgccagcca gccctatcgc agcatcacgc tcggcgagat cgctggcgag
     3841 gcaggcatca gcctcgccga tctgcgcgac ttgttcccct ccaagggcgc cattctcggc
     3901 ggtctgatgc gcatggtgga tcgcgaggtg ctgggcggca ctgctgccga catggcggac
     3961 gagaccgccc atgatcgggt gctcgatgtg atgctgcgcc ggttcgatgc actggctccc
     4021 tatcgcgacg gtttgcgctc ggtgtggaag gatctgtcaa ccagcccggc cgatctcatc
     4081 gccttcaacc aggccgccgt caattcctgg cgctacatgc tcgagagcgc cgcgatcgac
     4141 accaacggcc atcttggtgc cttgcgcacc cagggcgccg ttctggtctt tgctcgcgcc
     4201 tttgccacct ggctggacga tgaaccggac atggcccgca ccatggccgt gctggatcgc
     4261 gaactgaagc gcggtgcgtc agtcctcggc gcggccgacg cgctgcatcg gctctccgcc
     4321 ccgtttcgtg gcctgatgcg tgccgcctgc gatggtcgcc gcgcccgctc cgctccggcc
     4381 gcagctccgg aagcctgaat gaccgaaacg atcagttttg tcgatttcga gcgtgtcgac
     4441 atccgcgtcg gcacgatagt tgcggtcgac cccttccctg aggcgcgcaa gccggcctac
     4501 aagctgagga tagacttcgg gccggccctg ggcatccgca agtcgtcggc gcagatcaca
     4561 gcgcattatg caccggaaac gctgatcggc cgccaggtgg cggcggtggt caatttcccg
     4621 ccccgccaga tcggcaaatt catgtccgag gtgctgacgc tgggttttcc cgacgagagc
     4681 ggcgccgttg tgctgatcag cccgaccctt ccggttcccg atggcggcaa gctgttctga
     4741 accgctcacg ccggtaggcg gatgatcgca cgcaggccgc cgagcgggga tccatccagt
     4801 gtcacatccc cgccatgcgc ccgcgcgatg tcgcgcacga tcgacaggcc gagaccggtg
     4861 ccgccatgat cctggttgcg tgcttcgtcc agccgatgga acgggcggaa cacatcggcg
     4921 cgcttgtccg cgggaatgcc tggtccgtca tcgtcgatgg tgatggtcag atagcgctcg
     4981 tcatgcacgg cattgatctc gattcggtcg ccatagcgcg cagcatttga caccagattc
     5041 tgcaccagcc ggcggaacgc atcgggccgc accttgacca tcggatcgcc cgcgatggtc
     5101 agctgcgtgt tgtggccctc gcgctcggca ttggacttca gctcctccag caggccgcgc
     5161 atgtcggtcg gcaccgcact ttcaccggta tcaccgcggg cgaaggcaag ataggcttcg
     5221 agcatgcggg tcatttcgtc gacatcgcgc ttgagatcat cgacctccgg gctttcgtcg
     5281 agcagttcca gcgacagttt gaagcgggtc agcacggttc tcagatcatg gctgacgcca
     5341 ttgagcatgg cggtgcgctg gtcgatggcc cgttcgatgc gccgcttcat ttccaggaag
     5401 gcgatgcctg cccgcctgac ttccgaggcg ccgcgcggct tgtaatcgac atcgcgcccc
     5461 ttgccgaagc tttccgagcc gcagcaccgg gcggacctga ttgcgcagga acaggctggc
     5521 gatgacgatc aggatcaacg acgagccgac catccacacc aggaagatat ggctgttgga
     5581 ggcataggcc tggctgcgcc gggcaatgac tcgcatgatc gcatcttcga gctggacgcg
     5641 gatttcgatg acattggatt tcccgaccgt atcgatgaag aaaggccggt tgagctggcg
     5701 tcgcaactcg gtcgaaaggc ttgcgtcgag aatttcgaaa aacggcttgg gcagcgccgg
     5761 cggcagtgcc tctggcggca ggaaatcgat gtcgagcccc atcttttcca gcgcgatgcg
     5821 ctcgagttcc tcatggctgg accctggcgg cagcagccgg cgcatctcga ccagcgcggc
     5881 gatatcctgg gtcacggtgg ccgatagcct ctgggttacc agctgccagt gacgctccat
     5941 gaacgtatag gcgatcaccg attgcagcag caccatcggc gtgatgatga tcagcagggc
     6001 ccgcgcgaac agccccttgg gcatgcggcg cctgagccac agcgccgggt gccagcgcgc
     6061 aaagggcatc gcctgccaca gtttcatgaa tccggtccgt tctggcatgg gaatccctta
     6121 cggatcgacc accagccggt agccgacacc gcgcaccgtc tgcagatagg tcggattggc
     6181 ggcatccggc tcgatcttgc gccgcagccg gttgatctgc acatcgatgg tccgttcgtt
     6241 cgcgccgctg tcgggatcgg ccagcagttc ccgcgccacg gtggacccca gagcctgggc
     6301 gaactggcgc aggatatcgc gctcgcgatt ggtcagccgt accgtatcct cgccatgctt
     6361 cagttctccg cgccccagat ggaactggaa cgggccgaac cgcaccatct cggggccgac
     6421 cggagccgca gcaggcggaa cagcggcgac gcggcgcagg atactgttga cccgcagcag
     6481 cagttcgcgc ggatcgaacg gcttgccgag ataatcgtcg gcgcccagtt cgaggccctt
     6541 gagccggtca tcaagatcgg tcaaggccgt gagcatcagg atcggcacgg aagaggtctt
     6601 gcggatggca cgggccagat caaagccgct ctcgcccggc atcatcacat cgagcaccag
     6661 gagatcgaaa gcgagaccgt tcagatgctc gcgcgcctcg gcggcattgc cggcggtggt
     6721 caccctgtag ccgttcgact tgaggaattt cgacagcagc gagcggatgc ggctgtcgtc
     6781 atccacgacc aggacatgcg gcgcgtcatc gagaagcggc acaggcagcc ggaccatcac
     6841 cgcctcctct gatccagcgt caggcgtgtc agccgcgcca cctcgctgcg ctggtcggca
     6901 tcgatcaggc cgccgagaaa gccggtgacc gccgccacct ccgcctccga gagccgctca
     6961 agcgcccgtc ccatgcggcg tgtctgcaac cccagcaatt cctcggcaag ggccgtgccg
     7021 gtcgcgctca gatacatcag gcgctggcgc cggtcctgct ggccggcctt ctgcacgatg
     7081 aagccgccgg cgaccagatc acgcaggacg cggccaaggc tctgtttggt gatgtcgagg
     7141 atcgacagca attccgccac cgtcagcccc ggattgcggt tgacgaaatg cagcacgcga
     7201 tgatgcgccc ggccgaaacc gtagcgttcc agaacgcggt ccggctcgcc gataaaatcc
     7261 cggtaggcaa agaaaatcag ttcgaccagc gcgaccggat caagggttcc ccgcccttca
     7321 ggcgcagaaa ccgccgtcac cccgctgtcg cgcattgccg ctgccggcct gtcgttcatt
     7381 ccaaactcct cgcccgccag cacccgggcc catgaattat gtcagtctta ttgacttaat
     7441 tgcagggact gctacggtcc agactcagga tggttgccga aaagggacca taaaggctgt
     7501 tataggccgt ccggcccgtc gggaacaggg ccttggcgag ggagcgagat acgatgtcga
     7561 ttttgccttt tgatgatcgc gacgggttca tctggttcaa cggcaagctc atcccctggc
     7621 gtgaggccaa gctgcatgtc ctgtcgcacg gcctgcatta tggcggcgcg gtattcgagg
     7681 gcgaacgcgc ctacgccggc aagatctaca agtcccgcga acattccgag cggttcttga
     7741 agtccgccga acttctcgac ttcaccattc cctattcccc tgccgaactc tgcgccgcca
     7801 agcaggcggt gatcgatgcc aatggcggcg gcgatctcta cgtgaggccg gtagcctggc
     7861 gcggatcgga gatgatggcg gttgccgcgc agaacgcgac catccatgtc gccatcgccg
     7921 tctgggcctg gccgtcgatg ttcgacatca acaccaagat gaagggtatc cggctcgaca
     7981 tcgccgaatt ccgccggcct gatccggcca ccgcgccgtg tgcctccaag gcagcgggtc
     8041 tctacatgat ctgcacgatt tccaagcaca gggccgagcg caagggctat gccgacgcga
     8101 tgatgctcga ctggcagggc cgtgtcgccg aatgcaccgg cgccaacatc ttcttcaccc
     8161 gcgatggcgc gatccacacg ccgatcgccg attgcttcct caacggcatc acccggcaga
     8221 cggtgatcga catcgcccgc cgcgagggtt ttgaggtgat cgagcggcgg atcatgccgg
     8281 aggagctggc cggcttcaac gaatgcttca tcaccggcac ggctgcggaa gtgacgccgg
     8341 tgagcgagat cggcccctac aaattcgcgc cgggcaacat ctcccgctca ttgatcgagg
     8401 cctatacacg ggaagtcacg ccgacggctt gacccacgcc atcacgggcg tcagacatcc
     8461 gccgccgtga tcgccgccac atcgcagtcg ggatcatcga ccacccggcc cgcctgacgc
     8521 tccagcacct gctcgaacaa ttgccgtacc gaacggccgt tgccaaaatc cttggcccgc
     8581 gttgcataca tgtcggaaaa gcgcgccagc agtgcttcgc gggccgcctc gtcgaggctg
     8641 taaccctgcg cgacagcaag gcggctgaag atgtccatca tttcctcggg cgtgtaatcc
     8701 tcaaaagtga tggtgcgcga gaagcgtgac ttcagccccg gattgctgtc gatgaatccg
     8761 gcgatctcgt cggtatatcc ggcgatgatg acggcgagac gctcgcgctt gtcctccatc
     8821 tccttcagca gcgtgtcgat ggcttcctgc ccgaagtcgt tggagccggc gccgttgagc
     8881 gtataggcct cgtcgacgaa cagcacgccg tcgagcgctt ccttgatctt gtccttggtc
     8941 ttggccgccg tctggccgac atagccggcc accagcccgg cgcgatcggt ctcgaccaca
     9001 tgcccgcgcc gcaccagacc aagcgcggca tagatctcgc ccaccagccg ggcgaccgtg
     9061 gtcttgccgg tgccgggatt gccggtaaag acaagatgca ggctcatcgg cagcggcgcc
     9121 atgctctggg ccacgcgccg ttcgttgacg cgcgcgacat tgatgagttt tttcacttcc
     9181 gccttgaccc cgccaaggcc ggtcagcctg tcgagcttgt ccatgacatg atcgagatcg
     9241 agcttctggc caaaaccctg atcgggaatg tcggcggccc ggaacttcga cggatcggcc
     9301 tgcggatggg cgaacaggcg ctgcgcctgc cgctcgatca tcttttcgaa gaaggcgcgc
     9361 acctcgcgcg cattgccgaa ctgctcgtcg cgggtgcgat gcatctcctt cagcacgaaa
     9421 gcgatgcgct catgcgcatc ctcgccgatt tcgagcttct gctggcgcac cagcgcctcg
     9481 aagatctcca tcagttcctc ggcggtgtaa tcctcgaaat ggatcatccg ggtaaagcgt
     9541 gatttcagcc ccggattggc atcgatgaat gcccgcatct cgttgcgata gccggcgacg
     9601 atcaccgtca gccggtcgcg gtgatcctcc atcgctttca gcagcgtgtt gattgcctcg
     9661 acgccggtgt cggaactgct gccctgcttg gccagcgtat aggcttcgtc gatgaacagc
     9721 acgccatcga gggctttctc gatggcttcc ctggtctttt tctcggtgcc gccgacatag
     9781 ccttcgacca gcgaggaacg gtccacctcg accacatggc cggaggcgca aacgccgagt
     9841 tcgcgataaa tctcgccgat cagccgcgcc accgtcgtct tgccggtgcc cggattgccg
     9901 gtgaagacga gatgcgagga caccggcgcc accggcagcc cttgcgcgcg gcggcgctga
     9961 ttggccagca tcagcgcttt cagcgaagca agctcgcgct tgacctccgc cagaccgatg
    10021 agtttttcca gccgtt
//
LOCUS       DAOMXG010000085         9995 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_3570_length_9995_cov_9.315392, whole genome shotgun sequence.
ACCESSION   DAOMXG010000085 DAOMXG010000000
VERSION     DAOMXG010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 9995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9995
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3570_length_9995_cov_9.315392"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="PLQ11_07170"
     CDS             <1..295
                     /locus_tag="PLQ11_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HRK24718.1"
                     /translation="VPRLANTSLFAIPGLRAETSVIAFDLDGVAVSSGAACSSGKVTK
                     SHVLKAMGVADDLAAAAIRVSFGWTSTLAEVEACLSSLERQLARLRARPLSAA"
     gene            354..1823
                     /gene="sufB"
                     /locus_tag="PLQ11_07175"
     CDS             354..1823
                     /gene="sufB"
                     /locus_tag="PLQ11_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011975623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HRK24719.1"
                     /translation="MPAVKETVDHVRSIDVDQYKYGFVTNIESELAPKGLNDDIVRYI
                     SAKKGEPQWLLDWRLEAFHRWQTMVEPTWARVNYPAIDFQDLHYYAAPKKTPGPKSLD
                     EVDPELLRTYEKLGIPLREREVLAGVEGARVAVDAVFDSVSVVTTFKEELAKAGVIFC
                     SISEAVKEHPDLVRKYLGTVVPTTDNFYATLNSAVFSDGSFVYIPPGVRCPMELSTYF
                     RINEQKTGQFERTLIIADKGAYVSYLEGCTAPKRDENQLHAAVVELIALEDAEIKYST
                     VQNWYPGDSEGRGGIYNFVTKRGDCRGPRSKISWTQVETGSAITWKYPSCILRGEGSS
                     GEFYSIAISNGYQQVDSGTKMIHLGANTTSKIISKGIAAGKSDNTYRGLVSAHRKAKN
                     ARNFTNCDSLLIGNDCGAHTVPYIESKTATAVFEHEATTSKIAEDQLFYCMQRGLDEE
                     AAVALIVNGFVKDVLQQLPMEFAVEAQKLIAVSLEGSVG"
     gene            2011..2763
                     /gene="sufC"
                     /locus_tag="PLQ11_07180"
     CDS             2011..2763
                     /gene="sufC"
                     /locus_tag="PLQ11_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HRK24720.1"
                     /translation="MLEIKNLTVRIADTDTQILNGLNLTVKAGEVAAIMGPNGSGKST
                     LSYVISGKEDYEVTEGDILLNGESILEMEAHERAAAGLFLAFQYPLEIPGVATMTFLK
                     AAMNAQRKSRGEDEIGPADFMKAVRAAAAQLKIDPEMLKRALNVGFSGGEKKRMEILQ
                     MALLKPRFCILDETDSGLDIDALRIVSEGVNALRAKDRGMLVITHYQRLLDHIVPDTV
                     HVMARGRIVRSGGAELAHELEKNGYRDYVDAA"
     gene            2771..4075
                     /gene="sufD"
                     /locus_tag="PLQ11_07185"
     CDS             2771..4075
                     /gene="sufD"
                     /locus_tag="PLQ11_07185"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HRK24721.1"
                     /translation="MTVHVIETKAERALAEQFRAVLPHLPGMRERRAAAFATFEKTGL
                     PSRRLESWHYTDWKGRQRETLPLVEEPLADGLAIASGLVDPFSGIDAYNVLLVNNDTT
                     LLSEQPDQVDVVDLVEAARTGHPLMAHLGTAFPASADPMVALNEAMFVGGVVARLRKG
                     ARLVKPAHASATAAAMVPSSVHARSLIIVEDGVELTLLDSVTGRDDIDFQLSTVVEFI
                     IGDNAKVEYITYNRAGARSQAFIGVGVRMGRNSQFNTVNFAHGVGFGRHQVHVAMAGE
                     GSKLGVRGAALITGEQHFDHTLTVEHIAPGCESRELFRTVVDGQGTGVFQGKIVVRKE
                     AQKTDGQMASNALLLSDDATMNNKPELEIFADDVVCAHGATCGALDEELLFYLQARGL
                     PKKEAEALMVQAFIGEVLEFVSHEEARERLSGVVEGWLAGRG"
     gene            4079..4492
                     /locus_tag="PLQ11_07190"
     CDS             4079..4492
                     /locus_tag="PLQ11_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24722.1"
                     /translation="MAGRKTWREKMGPVKSPEVTTLARAMVGGRPGDRLLIPTPHLVR
                     DYVVAIPRGETRDVLRLRADLAEAAGADVTCPLTTGMFLRIVAEAALEDAGAGAPQEA
                     VTPFWRVVDPVSPLARKLSCGPAFIEARRHEEACA"
     gene            4489..5724
                     /locus_tag="PLQ11_07195"
     CDS             4489..5724
                     /locus_tag="PLQ11_07195"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HRK24723.1"
                     /translation="MTRHVPYDVAAIRRDFPALSLEPYGKRLVYLDNAASAQKPRVVI
                     DRMRQVYESEYANVHRGLHYLANAATEAYEGAREAVRAFLNAGSTDEIIFTKNATEGL
                     NLVASSLGRHLKIGAGDEIVLSIMEHHSNIVPWHFWRESAGAVIKWAPVDEDGNFLID
                     EFEKLLTPKTRVVSITQMSNALGTIVPVKEVVRIAHARGIPVVVDGSQAAVHLPVDVQ
                     DIGCDFYVITGHKLYGPSGIGALYGKRAWLDQIPPFLGGGEMIREVSEDRVTYGAPPH
                     RFEAGTPPIVPAAGLAAALGYINGLGKATIRAHEDDLVAYAHERLRAINSLRIVGQAR
                     SKGAIVSFSFPNAHAHDIATIIDRHGIAVRAGTHCTMPLLKRFGETATCRASFGLYNM
                     RDEVDALAEALIRAQRLFS"
     gene            5735..6106
                     /locus_tag="PLQ11_07200"
     CDS             5735..6106
                     /locus_tag="PLQ11_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HRK24724.1"
                     /translation="MNDFHEPNRPETAAAGTLPPEEIDQLTDVIIRALKTVYDPEIPA
                     DIYELGLIYRIEIQDDRHVNVDMTLTAPGCPVAGEMPGWVENAVNSCPGITGCTVRMV
                     FDPPWDQSRMSDLARVSLDMW"
     assembly_gap    6258..6267
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6268..7182)
                     /locus_tag="PLQ11_07205"
     CDS             complement(<6268..7182)
                     /locus_tag="PLQ11_07205"
                     /inference="COORDINATES: protein motif:HMM:NF014653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HRK24725.1"
                     /translation="MGQTNRTADELTDLTIRIFTAAGWSRQEAEDLAEHLVLANLSGH
                     DSHGVGMIPAYIQAWQDGLLSPRNRPEVRRDGAPFLVIDAKIALGQPAAKAAVGRAIA
                     MAGQSGVCLLNLINANHMGRIGHYAEMAAAAGMIGLFWVNVAGRPSVVASFGARESRF
                     GTNPHAIGIPNGAEPLILDFATSRMAHGKARVAFNKGERVPEGYLIDEQGKPTTDPAV
                     VQGARSGALLPFGDHKGAGIAIVVEILAAALAQGAIIPETPVKSWIINNLFGLIFDPA
                     RLDPNGTERERRIALLAAGGAIEAVDAVL"
     gene            7501..7884
                     /locus_tag="PLQ11_07210"
     CDS             7501..7884
                     /locus_tag="PLQ11_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HRK24726.1"
                     /translation="MGTGQRSLQVMQLTEAAAARIAEQIARSDSPVIGLRVGVEKGGC
                     AGMSYTMSYATELKPFDEVVESHGVKLIVDAKAVLFLLGTTLDVKADKFSSGFVFRNP
                     NETSHCGCGESVAIEPANADTLARA"
     gene            complement(7932..8327)
                     /locus_tag="PLQ11_07215"
     CDS             complement(7932..8327)
                     /locus_tag="PLQ11_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24727.1"
                     /translation="MRYGVLISLAVLALGAGHQQAQAGGLGTSPGLASDGTYSNWTRS
                     NHPYPWQYPSTYPFSTPVVYTAGPGAYYPAKRYRRHVARHGQRWTGYPPRHFYVVSYN
                     GYAPQYGFPPGAYQLPGYHPYHDHYGYYR"
     gene            complement(8483..>9995)
                     /locus_tag="PLQ11_07220"
     CDS             complement(8483..>9995)
                     /locus_tag="PLQ11_07220"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRK24728.1"
                     /translation="LEERRVAGSPLRPLFAVWLGSGPEQQARFRAAHIPAYDTEADAM
                     SGLMQLARIRRRSDALLRPPPDLPAQILPDREAALKAITSALDDGRRWLSQIEVYALM
                     HAYGIPLPPVVLARNAEEAAAQAATLAGAGKTFAVKIHSPDIVHKSDVDGVRLGLTSK
                     AQIVAAAADILARARRLKPDARIDGVTIQPMISRPHARELIVGIAVDPTFGPVALFGH
                     GGTAVEVIDDKALALLPLDLAQSRDLIRNTRISRLLAGYRNVPPVDSEKIAEVLVRVS
                     RLVEDNPEIIGLDINPLLANSEDVIGLDARVEVAPVDRDRRERLAGQRFAVRPYPRDL
                     EHQVTSRDGAHLRVRPMRPTDQAALTVMLQRTAPKDLRMRFLSSVNKLDPVMMARLTQ
                     LDYAREMAFVALDPVSAEILGVVRLHGDANRERGEYAILIRSDQQGRGIGRALMDSII
                     RFARNEGYAEISGEVLAENAGMLRMAAELGFAILRQPGEDTAMVRLQLAEARH"
BASE COUNT         1835 a         3162 c         3249 g         1739 t
ORIGIN      
        1 cgttccgcgc cttgccaata ccagcctgtt tgcgataccc ggcctcaggg ccgagacatc
       61 ggtgatcgcc tttgatcttg atggtgtggc ggtctccagc ggcgcggcct gctcgtccgg
      121 caaggttacg aagagccatg tgctcaaggc catgggcgtg gccgatgatc tggccgcagc
      181 cgctattcgt gtcagcttcg gctggacctc gacattggcc gaggtcgaag cctgcctgtc
      241 cagccttgag cggcagcttg cccggctcag ggcgcgcccg ctttcggcgg cgtgacgtgc
      301 cttgaatgac aaccccaccc cccggccctt gaaaccggag aggagaggac acaatgcctg
      361 ctgtgaaaga gacggtcgat cacgtccggt cgatcgacgt cgatcaatac aaatacgggt
      421 tcgtcaccaa tatcgaatcc gaactggctc caaagggcct caacgacgat attgtccgct
      481 acatctccgc caagaagggt gagccgcaat ggctgcttga ctggcggctg gaagcgttcc
      541 accgctggca gacgatggtg gagccgacct gggcgcgggt gaattatccc gccatcgatt
      601 ttcaggatct gcattactac gcggcgccga aaaagacgcc ggggccgaag agcctcgacg
      661 aggtcgatcc cgagctgctc aggacctatg aaaagcttgg catcccgctg cgcgagcgcg
      721 aggttctggc cggcgtcgag ggcgcgcgcg tcgcggtcga tgcggtgttc gattccgtct
      781 cggttgtcac caccttcaag gaggaactgg ccaaggccgg cgtcatcttc tgctccattt
      841 cggaagcggt gaaggaacat cccgatctgg tgaggaagta tctcggcacg gttgttccga
      901 ccaccgacaa tttctacgcg acactgaaca gcgcggtgtt ctcggatggc tcgttcgtct
      961 acatcccgcc gggcgtgcgc tgcccgatgg aactgtcgac ctatttccgc atcaacgagc
     1021 agaagacggg gcagttcgag cgcacgctga tcatcgccga caagggcgct tacgtgtcct
     1081 atctcgaagg ctgcaccgcg ccgaaacgcg acgagaacca gctccacgcc gccgtggtcg
     1141 aactgatcgc gctcgaagac gccgagatca agtattccac cgtgcagaac tggtatcccg
     1201 gcgacagcga gggcaggggc ggcatctaca acttcgtcac caagcgcggc gattgccgcg
     1261 gcccgcgctc gaagatttca tggacgcagg tggagaccgg ctcggccatc acctggaaat
     1321 acccctcctg catcctgcgc ggcgagggct cctccggcga gttctattcc atcgccatct
     1381 ccaacggcta ccagcaggtc gactcgggca ccaagatgat ccatctgggc gccaacacga
     1441 cgtccaagat catctccaag ggcatcgcgg cggggaaatc cgacaacacc tatcgcggtc
     1501 tggtctcggc gcaccgcaag gcgaagaatg cgcgcaactt caccaattgc gacagcctgc
     1561 tgatcggcaa cgattgcggc gcccacaccg tgccctacat cgagagcaag acggcgaccg
     1621 ccgtgttcga gcacgaggca accacctcga agatcgccga ggatcagttg ttctactgca
     1681 tgcagcgcgg cctcgacgag gaggcggcgg tggcgctcat cgtcaacggc ttcgtcaagg
     1741 acgtgctcca gcaactgccg atggaatttg cggtggaagc gcagaagctg atcgccgtga
     1801 gccttgaggg ttcagtcggc tagagcatgc tgcgattggg tggaacccgg ctaatcgcaa
     1861 caagcatgct ctaaacactc gataacgatc acgtatctct cgtttttgat gattccatca
     1921 aaacgagagc gtgatctggg aacagaattt ggaagtcgtg gatggtcgga acaagtccga
     1981 ccatgacgac agatgcggaa gaagaacacg atgctcgaaa tcaagaacct caccgtccgc
     2041 atcgcggaca ccgacaccca gatcctcaac ggcctgaacc tgacggtgaa ggcgggcgag
     2101 gtggccgcca tcatggggcc gaacggctcg ggcaaatcga ccctgtccta cgtcatctcc
     2161 ggcaaggagg attacgaggt caccgagggc gacatcctgc tcaatggcga gagcatcctt
     2221 gagatggaag cgcatgagcg cgccgccgcc gggttgtttc tggccttcca gtatccgctg
     2281 gaaattccgg gcgtcgcgac catgaccttt ctcaaggcgg cgatgaacgc ccagcgcaag
     2341 tcgcgcggcg aggacgagat cggcccggcc gatttcatga aggcagtgcg cgccgccgcc
     2401 gcccagctca agatcgaccc ggagatgctc aagcgcgcgc tcaacgtcgg cttttcgggc
     2461 ggcgagaaga agcgcatgga aatcctgcag atggcgctgc tcaagccgag attctgcatc
     2521 ctcgacgaga ccgattccgg cctcgacatc gatgcgctga gaattgtctc ggagggcgtc
     2581 aacgccctgc gcgccaagga tcgcggcatg ctggtgatca cccattacca gcgcctgctc
     2641 gatcacatcg tgcccgatac cgtgcatgtg atggccaggg gccggatcgt gcgctcgggc
     2701 ggggcggaac tggcgcacga actcgagaag aacggctatc gcgactacgt ggacgcggcg
     2761 tgaggcggtg atgacggttc atgtgatcga gaccaaggcc gagcgcgcgc tcgccgagca
     2821 gttccgggcc gttctgccgc atcttccggg gatgcgggaa cggcgcgcgg cggcattcgc
     2881 gaccttcgaa aagaccggtt tgccgtcgcg gcggctcgaa tcctggcatt ataccgactg
     2941 gaagggccgg cagcgcgaaa cgctgccgct ggtcgaggag ccgctggccg acggtctggc
     3001 gatcgccagc gggcttgtcg atccgttcag cggcatcgac gcctacaatg tcctgctggt
     3061 caacaacgac accaccttgt tgtcagagca gcccgatcag gttgatgtgg tcgatctggt
     3121 cgaggccgca cgcaccggcc atccgcttat ggcgcatctc ggcacagcat ttcccgcaag
     3181 cgctgatcct atggtggcgc tgaacgaggc gatgttcgtc ggcggcgtcg ttgcccggct
     3241 gcgcaagggc gcgcggctgg tcaagccggc gcatgccagc gcgaccgccg ctgcgatggt
     3301 cccaagttcg gttcatgcac gctcgctgat catcgtcgag gacggcgtcg aactgacgct
     3361 gctcgacagt gtgaccggcc gtgacgatat cgacttccag ctctccacgg tggtcgagtt
     3421 catcatcggc gacaatgcca aggtcgaata catcacctac aaccgggccg gtgctcgctc
     3481 ccaggccttc atcggcgtcg gtgtgcggat ggggcgcaat tctcagttca acaccgtcaa
     3541 cttcgcgcat ggcgtcggct tcggccggca tcaggtgcat gtggcgatgg cgggcgaagg
     3601 ctcgaagctg ggcgtgcgcg gcgcggcatt gatcaccggt gagcagcatt tcgatcatac
     3661 cctgacggtc gagcacatcg cgccgggctg cgaaagccgc gaattgttcc gaacggtggt
     3721 ggatggccag ggaaccggcg tgtttcaggg caagatcgtc gtgcgcaagg aagcgcaaaa
     3781 aaccgacggg cagatggcct ccaacgcgct gctgctgtcc gacgacgcca ccatgaacaa
     3841 caagccggaa ctggaaatct tcgccgacga cgtggtctgc gcccatggcg ccacctgcgg
     3901 tgcgctcgat gaggagctgc tgttctatct gcaggcccgc ggcctgccga agaaggaggc
     3961 cgaggcactg atggtgcagg cgttcatcgg cgaggtgctg gaattcgtca gccacgagga
     4021 ggcgcgcgag cggctgagcg gcgtggtcga aggctggctt gcgggacggg gctgatcgat
     4081 ggcggggcgc aagacctggc gcgagaagat gggaccggtc aagtcgccgg aggtgaccac
     4141 gctggcgcgc gcgatggtcg gcggcaggcc gggcgaccgg ctgctgattc cgacgccgca
     4201 tctggtgcgc gattatgtcg tcgcgattcc gcgcggcgag acacgcgacg tgctgcggct
     4261 gcgcgccgat ctggcagaag ccgccggcgc ggacgtgaca tgcccgctga ccaccggcat
     4321 gttcctgcgc atcgtcgccg aggcggcgct ggaggatgcc ggcgccgggg cgccgcagga
     4381 ggccgtcacg ccgttctggc gcgttgtcga tccggtctcg ccgctggcca gaaagctgtc
     4441 ctgcggcccc gccttcatcg aggcgcggcg acacgaggag gcatgcgcat gacccggcat
     4501 gtcccctatg acgtcgccgc gatccgccgc gattttccgg ccctgtcgct ggagccctac
     4561 ggcaagcggc tcgtctatct cgacaatgcc gcctcggcgc agaagccgcg cgtcgtgatc
     4621 gaccggatgc ggcaggttta tgaaagcgaa tatgccaatg ttcaccgtgg tttgcattat
     4681 cttgctaatg cggccacgga agcctatgag ggcgcccgcg aggcggttcg ggcgttcctc
     4741 aatgccggat cgaccgacga gatcatcttc accaagaatg ccaccgaggg tctcaacctg
     4801 gtggcatcct cgctgggccg gcacctgaag atcggcgcgg gcgacgagat cgtcctgtcg
     4861 atcatggagc atcattccaa tatcgtgccc tggcatttct ggcgcgaaag cgccggcgcc
     4921 gtgatcaaat gggcgccggt ggacgaggat ggcaatttcc tcatcgacga attcgagaaa
     4981 ctgctgacgc cgaaaaccag ggtcgtgtcg atcacgcaga tgtcgaatgc gctgggcacc
     5041 atcgttccgg tgaaggaggt ggttcgcatc gcccatgcgc gcggcattcc ggtagtggtc
     5101 gatggctcgc aggccgccgt gcatctgccg gtcgatgtgc aggatatcgg ctgcgatttc
     5161 tacgtcatca ccggccacaa actctatggc ccgtcgggca tcggcgcgct ttatggcaag
     5221 cgcgcgtggc tggaccaaat tccgccgttt ctcggtggcg gcgagatgat ccgcgaagtc
     5281 agcgaggatc gggtgaccta cggtgcgccg ccgcaccggt tcgaggcggg cacgcccccg
     5341 atcgtgccgg cggcggggct tgccgccgcg cttggctata tcaatggcct gggcaaggcg
     5401 acgatccgcg cccacgagga tgatctggtg gcttacgcgc atgagcggct gcgcgcgatc
     5461 aacagcctgc gcattgtcgg tcaggcgcgg agcaagggag cgatcgtctc cttctccttc
     5521 cccaatgccc acgcccatga catcgctacc atcatcgatc gccacggcat tgcggtgcgg
     5581 gcgggcaccc attgcaccat gccgttgctc aagcgcttcg gcgagacggc gacctgccgc
     5641 gcctcattcg ggctctacaa catgcgcgat gaggtcgatg cgctggccga ggcgctgatc
     5701 cgcgcccagc ggctgttttc gtgaggggga agccatgaac gactttcacg aacccaaccg
     5761 cccggaaacg gccgccgccg gcacccttcc gcccgaggaa atcgatcagc tgaccgatgt
     5821 catcatccgc gcgctgaaaa ccgtctacga tccggaaatc cccgccgata tctacgaact
     5881 cgggctgatc taccgcatcg agatccagga tgaccgccac gtcaatgttg atatgacgct
     5941 gaccgcgccg ggctgtccgg tggccggcga gatgcccggc tgggtcgaga acgcggtcaa
     6001 ttcctgtccg ggcattaccg gttgcacggt gcgcatggtg ttcgacccgc cgtgggacca
     6061 aagccgcatg tcggatctcg cccgcgtctc gctcgatatg tggtaagcgt tcagaatgtg
     6121 gcgccgaccg cctcggccgc cgccatcagt tgccgccagg tttcatcatc cacgggaatg
     6181 ccgtcccggt tgcgctcggt gcgcacctcg cgctccctgt cgccgggcgc gcgcaccctg
     6241 tcgatgccat cggccggnnn nnnnnnngag cacggcgtcg accgcctcga tggcaccacc
     6301 ggcggcgagc agggcgatcc gcctttcccg ctcggtgccg ttcggatcga ggcgtgcggg
     6361 gtcgaagatc aggccgaaca ggttgttgat gatccagctc ttgaccggcg tctccggaat
     6421 gatcgccccc tgcgccagag cggcagcgag aatctcgacg acaatcgcga tgccggcgcc
     6481 cttgtgatcg ccgaacggca gcagcgcgcc ggagcgtgcg ccctgcacga cggcggggtc
     6541 ggttgtgggc ttgccctgct cgtcgatcag atagccttcc ggcacgcgct cgcccttgtt
     6601 gaaggcaacg cgagccttgc catgcgccat acgcgaggtg gcgaaatcga ggatcagcgg
     6661 ctccgccccg ttgggaatgc cgatggcatg cggattggtg ccgaaccgcg attcccgggc
     6721 accgaacgag gcaactaccg aggggcgccc cgccacattg acccagaaca ggccgatcat
     6781 tcccgccgcc gcggccatct cggcataatg gccgatccgt cccatgtggt tggcattgat
     6841 caggttgagg aggcagacgc cgctctgtcc ggccatggcg atggcgcggc caacggccgc
     6901 cttggccgcc ggctggccaa gcgcgatctt ggcgtcgatg accagaaacg gcgccccatc
     6961 gcgccgcacc tccggcctgt tccggggaga gagcagcccg tcctgccagg cctggatata
     7021 ggccgggatc atgccgacgc catggctgtc atgacctgaa agattggcca gaaccagatg
     7081 ctcggccaga tcctccgcct cctgccgtga ccagccggcc gccgtgaaaa tccggatcgt
     7141 caggtcagtc agttcatcgg cggttcggtt cgtctggccc atcatccggc tccggcttca
     7201 tggtcaagct ctgcatgctc tatgtcagcc ccggccatct ggcagcaacc gaaatcagcc
     7261 gcagcgccag gccatggaaa tgcggatcta gagcatgtcg cgatcaagtg gataccggtt
     7321 aatcgcagaa gccatgctct gacttattga taccgaacac gttatccgca tttagatgat
     7381 tccatcaaaa tgcggcgtga tctaatgcgc ttgcgggacc tcgaccccgg cgccaatcgt
     7441 gactatattg ccttgtctgt gtcatcgggc cttgaacccg gatcggcagg agagacgagt
     7501 atgggaactg gccagcgttc attgcaggtc atgcagctga cagaggcggc ggcggcgcgc
     7561 atcgccgagc agatcgcccg ttcggacagc ccggtgatcg gcctgcgtgt cggagtggag
     7621 aagggcggct gcgccggcat gtcctacacg atgtcctacg ccaccgaatt gaagcccttc
     7681 gacgaggtgg tggaaagcca tggcgtcaag ctgatcgtcg atgccaaagc ggtgctgttc
     7741 cttctcggca ccacgcttga tgtcaaggcc gacaagtttt cctcgggctt tgtctttcgc
     7801 aatcccaacg agacctcgca ctgcggctgc ggcgaatcgg tggccatcga gccggccaac
     7861 gcggacacgc tcgcccgggc ctgaacgcca gggcgcattg ctgtttccgg ccgcgagcgc
     7921 cggccggccg tttagcgata ataaccgtag tggtcgtggt agggatggta gccgggcaac
     7981 tggtaggcgc ccggcgggaa gccgtattgc ggcgcatagc cgttgtagct caccacgtag
     8041 aaatgtcggg gcgggtatcc cgtccagcgc tggccgtgcc gggcaacatg gcgccgatag
     8101 cgcttggccg gataataggc gcccggcccc gccgtataga cgaccggggt cgagaatgga
     8161 taggtggacg gatactgcca cgggtagggg tggttggagc gcgtccagtt ggaataggtg
     8221 ccatcactgg cgagcccggg tgatgtgccg aggcccccag cctgcgcctg ctggtggccc
     8281 gccccaagcg ccagaaccgc cagtgaaatc aaaacgccat aacgcatggt gccgaacccc
     8341 ttcccacaca ggttgtcatc ggcactctgg gcatgggcca gcgagcggtt tgctaacaac
     8401 ggcggcggag gcagactttt ctcgccggca ggctgaacaa agccctcaca ggtaacatta
     8461 atggcccgtt gccccgtccg gatcagtgcc gcgcctcggc cagttgcaga cgcaccatcg
     8521 ccgtatcctc tcccggctgc cggaggatgg caaagcccag ttctgccgcc atgcgcagca
     8581 tgccggcgtt ctcggccaga acctcgccgg aaatctcggc atagccctcg ttcctggcaa
     8641 agcggatgat gctgtccatc aaagcccggc cgatgccgcg cccctgctgg tccgagcgga
     8701 tgaggattgc atattccccg cgctcccggt tggcatcgcc atgcaggcga acgacgccga
     8761 ggatttcggc gctgaccgga tcgagcgcca cgaaggccat ttcccgcgca tagtcgagct
     8821 gggtcaggcg ggccatcatc accggatcaa gcttgttgac ggaggacagg aaacgcatcc
     8881 gcagatcctt gggggcggtg cgttgcagca tcaccgtcag cgccgcctga tcggtcggtc
     8941 gcatcggcct gacgcgcaga tgcgctccat cgcgcgaggt cacctgatgt tcaaggtcgc
     9001 gcggataggg acggacagca aaacgctgcc cggccagccg ttcgcgccgg tcccgatcca
     9061 ccggagccac ctcgacgcgg gcatcaagcc cgatcacatc ctcggaattg gccagcagcg
     9121 ggttgatgtc gagcccgatg atctccggat tgtcctcgac cagtcgggaa acgcgcacca
     9181 gaacctcggc gatcttctcg ctgtcaaccg gcgggacatt gcgatagccg gcgagcaggc
     9241 gggaaatccg ggtgttgcgg atgagatcac gcgactgggc aagatcgagc ggcagcagcg
     9301 ccagcgcctt gtcatcgatc acctcgaccg ccgtcccgcc atggccgaac agcgccaccg
     9361 ggccgaaggt gggatcgacc gctatgccga cgatcagttc gcgcgcatgc ggccgggaga
     9421 tcatcggctg gatggtcacc ccgtcgatcc gcgcatcagg cttgaggcgg cgggcacggg
     9481 ccagaatgtc ggccgctgcg gccacgatct gcgccttgct ggtcagtccc agccggacgc
     9541 catcgacatc ggatttgtgc acgatatcgg gcgaatggat cttgaccgcg aacgtcttgc
     9601 cggcgccggc cagtgtcgcg gcctgggcgg ccgcctcctc ggcgttccgg gcgagaacaa
     9661 ccgggggcag gggaatgcca taagcgtgca tcagcgcata gacttcgatc tgcgacagcc
     9721 agcggcggcc gtcatcgagc gctgaggtga tcgccttcag tgccgcttcg cgatctggca
     9781 ggatctgcgc cggcaggtcc ggcggcgggc gcagcagggc atcgctgcgg cgacggatgc
     9841 gggccagctg catcaggccg gacattgcgt cggcctcggt atcataggcc gggatgtggg
     9901 cggcgcggaa ccgtgcctgc tgctccgggc cggaccccag ccagacggca aacagcggcc
     9961 gcagcggcga cccggcgacg cgccgttcct ccagc
//
LOCUS       DAOMXG010000086         9896 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_3617_length_9896_cov_9.765268, whole genome shotgun sequence.
ACCESSION   DAOMXG010000086 DAOMXG010000000
VERSION     DAOMXG010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 9896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9896
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3617_length_9896_cov_9.765268"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1006
                     /locus_tag="PLQ11_07225"
     CDS             <1..1006
                     /locus_tag="PLQ11_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRK24729.1"
                     /translation="GLLAAAVAEPAGDAATISSLKLCLARETLAWHDGLADFAFAMQG
                     LGSGAIGLAGTPLMRRTVLPRTRSGEWIAAFALSEKDAGSDVAALRCTARREGASFVL
                     DGEKTWISNGGIADVYCVFARTGEAPGARGLSAFVVFADDPGFSIAERIDVMAPHPLA
                     TVRLDQCRIPADRLIGAPGEGFKVAMRTLDIFRASVAAAANGFARRALDEALAHAAQR
                     RMFGGCLADLQLTQAAFGDMATAVDAAMLLTYRAAWRRDIQHLPTTREAAMAKMAATE
                     TAQQVIDRALQMFGGRGVVRGERVEQLYREIRALRIYEGATEVQKLIIARDLLKAARP
                     "
     gene            complement(1136..2422)
                     /locus_tag="PLQ11_07230"
     CDS             complement(1136..2422)
                     /locus_tag="PLQ11_07230"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018095976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRK24730.1"
                     /translation="MRQTFPHYPDACGWDALLPPREPRAPAKGSLRVKYAVIGAGYTG
                     LAAARRLNELDPEAAILVLEAMNVGEGSSARNSGFANPRDSAFGLSPADVARGERLNR
                     YATEGFNWLLALMQQYGFDCDLTLTGRITAAATPAGEAKVRSLLDGARLQGVVHSHLD
                     ADGLAQWIGSRYYRSGFRAEEGYLLQPAKLIRGLAACLPEAITLCEHSPVLSLEKAGA
                     WRLATPESQIVADVVVMAANASIKHFGYLRDRVVTIYTYAGITEEMPPEDAARLGTAA
                     AWGVLPAHRLGTTVRRVGPNRLMVRSLYAYERGLSQETVRTALTSCFHRRYPELAHVK
                     LQYVWGGTTGLTMNGAPNWGRFDDNLYGFAGCNGSGIVKGTVLGKRLAEMICGTPQPD
                     LESAYGRADWIAPEPFRTLGFHVISAIERRKAGAEM"
     gene            2600..3928
                     /locus_tag="PLQ11_07235"
     CDS             2600..3928
                     /locus_tag="PLQ11_07235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013761256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRK24731.1"
                     /translation="MPIVMETLSHQESLPSRVDVVIIGGGIIGMTAALFLAQRGIPVA
                     ICEKGEVGHEQSGRNWGWVRVMGRDPAEIPMALEAQRLWEDMATILGEDVGFARAGIV
                     YVAETEAERAEQEAWLDQTAAYQLGTRRLRPSEIEAVLPGSRRRYAGALFTPEDARAE
                     PWKAVPAMARTVRRLGGHVLTRCAVRGIETAAGRVSGVITESGRIACQMAVLAGGAWS
                     RLFLGNMGIDFPQLRILGSVLQTEPLDGPPVHAVGASDYAFRKRSDGGYTIARRGASE
                     AHIVPDSFRLFVDFLPALYKQRREIRLALGNRFMEEARTPRRWSLDQVSPFEKTRILD
                     PEPSQKLLDEARRAVIAAFPAFSGMKIANAWGGLIDATPDAVPVIGEVPRLAGFFIAS
                     GFSGHGFGIGPGAGRLVADLVRGATPSVDPAPFRLARFARLARTTAPGIP"
     gene            4023..5570
                     /locus_tag="PLQ11_07240"
     CDS             4023..5570
                     /locus_tag="PLQ11_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020046854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRK24732.1"
                     /translation="MYRTHLAAGLAALVISAPAMAQKTTLVVGMQTQDAGVLDPHLNS
                     GTPGKALMNWMFNGLVRIKPGQASPDHIEPDIAESWTSSPDGKEWTFKLRQGVQCHHD
                     NGEFTSEDAVYSLNRAATKDSSAFSSDYAAFDKVEAVDKYTVKITLKQPIPSLLGQVM
                     SYHGGNMVCKKAAEALGKDGFGKKPAGTGPFMFAEYVPQQYVKLVANPKYFRGEPKLK
                     EIIYRFIPSDSARDLAFQAGEIDMMIGRQEQSWVDRVSATPGTKVIAFGPAEMSVLHL
                     NMTQPPLDNIKVRQAIAHAVDRAGIWNFRGKNSSRQAVSVVPSGYAGTLENAPLLPHD
                     LAKAKALLAEAGFPNGVTIKAIHTTLPGMFTFIEAVQAQLKRAGITLDITPVEHPTFH
                     QQIRQDLSQLVHFQAARFPIADVYLTQFFHSRSIVKTPSAVTNFSHCKVADAEIDAAR
                     TEQDKAKQTALWHEAQKKIIAEVCAVPVNEMLMIWAFKDSLDLGYDLKGSLNLGPPVT
                     EQTRFTK"
     gene            5831..6769
                     /locus_tag="PLQ11_07245"
     CDS             5831..6769
                     /locus_tag="PLQ11_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017269019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRK24733.1"
                     /translation="MGGFLLKRLIFSVVTLFAVLTLVFLIVRVVPGDPAQVILGDQAD
                     AAAIAALRGRLGLDRPVLEQYFSFLGGAVKGDWGVSLVTGRPVIREVLDVLPWTLELT
                     FVSMVLGTAIGVPLGVWAAINRNRMPDYVTRIASLIGLSFPPFVSAILLLISFSLVLR
                     WFPVISANRATPWQWFQSIALPALNLGLIMAAYITRVTRSAMLEVMQEDYVRTARAKG
                     APWRAIVWRHALRNALIPIVTIVGLYLGILIGNSVLTEIVFNRPGLGKLIIGALSQRD
                     YTMLQGMIVIYTLIVVLVNVATDLTYAFVDPRVKLK"
     gene            6766..7659
                     /locus_tag="PLQ11_07250"
     CDS             6766..7659
                     /locus_tag="PLQ11_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003523912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRK24734.1"
                     /translation="MSTSVHETSNAKASRTPRWLKATIAAFNANKTSWIGLVIFVLVV
                     ILALLAPWISPHDPVEQDVLSKLKPPFGNYYLGTDYYGRDILSRLLYGARISLVIGIA
                     STVLALVIGSMIGIVAGYYGGRIDVVFMQTLDMLHSFPSLILGLILVAMLGPTMTNII
                     IAIALTSIPSFARIARAPTISVKEREFIDAGRALGFSDTRLMAGHILPNIFPEILVMG
                     SLWLANAIRTEASLAFIGLGVKPPTPTWGGMIREGFENILDSYWLVLAPSIAILVIVL
                     ALQILGDGLRDAIDPKLKGEQ"
     gene            7656..9389
                     /locus_tag="PLQ11_07255"
     CDS             7656..9389
                     /locus_tag="PLQ11_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRK24735.1"
                     /translation="MSGAVLSVRDLVTEFRVDGQWRRAVDSVSFEIGAGETVAIVGES
                     GSGKSVTALSVMRLISPVNGRVSGEIRLEGQELLCLPEDAMREVRGNRISMIFQEPMT
                     SLNPVLTIGQQICEALQYHRDMSAREAEAEAIRILDRVRIPAARQRFGEYPHRFSGGM
                     RQRVMIAMALACRPRLLIADEPTTALDVTIQAQILDLIRSLQQEEGMSVLFITHDMGV
                     VAEIADRVVVMWKGRKLEDEPAVPIFARPQHGYTRALLAAVPKLGEMDAHPRPLRFPV
                     IEPSTGAVVTPASLVPETVRPGEPLLQVRGLTTRFPIRSGLFGRIGGRVHAVEDVTFD
                     LAAGETLALVGESGCGKSTTGRSILRLVEPTEGQVQFAGLNPRKTIGAAIAEPLTVHG
                     LADRKGAAERTAELLRRVGLLPDMATRFPHEFSGGQRQRIAIARALALSPELVVADEA
                     VSALDVSVKAQVLNLMLDLQQELGLAYLFISHDMAVVERVSHRVAVMYLGEIVEIGPR
                     RQVLSDPHHPYTRKLLAAVPVPDPGRRRSREPLAVEELPSPIRPADWVRPQSTLVEVG
                     PDHFVRVAAGI"
     gene            complement(9439..>9896)
                     /gene="mog"
                     /locus_tag="PLQ11_07260"
     CDS             complement(9439..>9896)
                     /gene="mog"
                     /locus_tag="PLQ11_07260"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HRK24736.1"
                     /translation="AIEAFLKEVLTSPFTIETRIVPDDRAAIEAAVRAFCDQAGAALV
                     LTTGGTGPAPRDVTPEAVHAVYDRELAGFGEAMRAASLNEVPTAILSRQSAGTRGKSL
                     IITLPGNPGAIRTCLMAVFAAVPYCIDLIGGPRLETDPSICKAFRPKGA"
BASE COUNT         1656 a         3212 c         3211 g         1817 t
ORIGIN      
        1 gggcctgctc gcggcggcgg tggccgaacc ggcaggcgat gccgcgacca tttcgtcgct
       61 gaaactgtgc cttgcccgtg aaaccctcgc ctggcatgac ggcctggccg atttcgcttt
      121 cgccatgcag gggctggggt ctggcgcgat cgggctggcc ggcacgccgc tgatgcgccg
      181 gacagtattg ccacggacac ggtcgggcga gtggatcgcc gcctttgccc tgtcggagaa
      241 ggatgccggc tcggatgtcg cggcgctgcg ttgcacggcg cggcgggaag gcgcgtcctt
      301 tgttctcgac ggcgaaaaga cctggatttc caacggcggc attgccgatg tctattgtgt
      361 ctttgcccgc accggggagg cgccgggcgc gcgcggcctt tcggcattcg tggtctttgc
      421 cgatgatccg gggttctcga ttgccgagcg catcgatgtg atggcgcccc atccgctggc
      481 gaccgtgcgg cttgaccaat gccgcatccc ggctgaccgg ctgatcggcg caccgggcga
      541 gggcttcaag gtcgccatgc gcacgcttga catcttccgc gcctcggtcg cggcagcggc
      601 caatggattt gcccgccgtg ccctggacga ggcgctggcc catgcagcac agcgccggat
      661 gttcggcggg tgccttgccg acttgcagct gacccaggct gccttcggcg acatggcgac
      721 cgctgtcgat gccgcgatgc tgctgaccta tcgtgcggcc tggcgacgcg acatccagca
      781 tctgccgacg acgcgcgagg cggcgatggc caagatggcg gcgaccgaaa ccgcccagca
      841 ggtcatagat cgggcgctgc agatgtttgg cgggcgcggc gtcgtcaggg gcgagcgtgt
      901 ggagcagctt taccgtgaga tccgcgcgct gcgcatctac gaaggggcga cggaagtgca
      961 gaagctgatc atcgcccgcg acctgctgaa agccgcgcgg ccctgatccg cccacctgag
     1021 cattttcaag ccaagtggaa tccggctcgc atcaagaaaa tacgatgaaa caaaaggata
     1081 gagcggttga tctgattcag tcagattgga aacaggcgct atcggcgcgg acgcgctaca
     1141 tctccgcgcc ggccttgcgg cgctcgatgg ccgagatcac gtggaaaccg agggtgcgga
     1201 acggctcggg cgcaatccag tccgcccgac cataggcgct ttcgagatcc ggctgtggcg
     1261 tgccgcagat catctcggcc aggcgcttgc caagcactgt tcccttgacg attcccgagc
     1321 cgttgcagcc ggcaaaaccg tagagattgt cgtcgaaacg cccccagttg ggcgccccgt
     1381 tcatcgtcaa cccggtggtg ccgccccaga catattgcag tttcacatgc gccagctccg
     1441 gatagcggcg gtggaagcat gaggtcagcg ccgtgcgcac ggtttcctgc gataggccac
     1501 gctcataggc atagagcgag cgcaccatca gccggttcgg cccgacacgg cgaaccgtcg
     1561 tgccgaggcg gtgggctggc agcacacccc aggcggccgc tgtgccgagc cttgccgcat
     1621 cctccggcgg catttcctcg gtaattccgg cataggtgta aatcgtcacc accctgtcgc
     1681 gcagatagcc gaagtgtttg atcgaggcgt tggcggccat caccacaaca tccgcgacga
     1741 tctgcgactc aggcgtcgca aggcgccagg caccggcctt ctccaaactc aggacggggg
     1801 agtgctcgca cagggtaatc gcttcgggca agcaagcggc cagcccgcgg atcagtttgg
     1861 ccggttgcag caggtagcct tcctcggcgc ggaagccgct ccggtaatac cggctgccga
     1921 tccattgcgc caagccatcg gcatcgagat gggaatggac gacgccttgc aaccgcgccc
     1981 cgtcgagcaa gctgcgcacc ttggcctcgc cggccggggt tgcggcggcg gtgatccgcc
     2041 cggtcagtgt cagatcgcag tcaaagccgt attgctgcat cagcgccagc agccagttga
     2101 acccttctgt tgcatatcgg ttgagccgtt cgccgcgcgc gacatcggcg ggcgacaggc
     2161 cgaaggcaga gtcgcgcggg ttggcaaatc ccgaattgcg ggcggaggaa ccctcgccca
     2221 cattcattgc ctccaggacg aggatggcag cctccggatc aagctcgttg aggcggcgtg
     2281 cggcggccag tccggtatat cccgccccga tgacggcata tttgacccgc agcgagcctt
     2341 tggccggcgc gcgcggttca cgcggcggca acagggcgtc ccagccgcag gcatccgggt
     2401 agtggggaaa cgtctggcgc atcagcgatc cgggatgatg acagtgaacc cggagagcgt
     2461 gccggcaatc accatccggc gcaatagctg atcgcaagcc cgcggcaatg aagtggcctg
     2521 ggctgatcgt cttgcctttc cggctttgac ttatacgatg accggcaagg gtcgcgcagc
     2581 gaatgggtca ggggtgcgca tgccaatcgt gatggagacg ctgagtcatc aggaatcgct
     2641 gccgtcgcgc gttgatgtcg tgatcatcgg cggcggcatc atcggcatga cggcggccct
     2701 gtttcttgcc cagcgaggga tcccggtcgc aatctgcgaa aagggcgagg tcggccacga
     2761 gcaatccggg cgcaactggg gctgggtccg ggtcatgggc cgcgatcctg cggaaatccc
     2821 gatggcgctt gaggcgcagc gcctgtggga agacatggcc accattctgg gcgaggatgt
     2881 cggctttgcc cgcgccggaa ttgtctatgt cgccgaaacc gaggcagaac gggcggaaca
     2941 ggaagcctgg cttgatcaga ccgccgccta tcaattggga acgcggcggc tgcgcccgag
     3001 cgaaatcgag gcggtgttgc ccggatcgcg acggcgttac gccggagcgc tgttcacccc
     3061 tgaggacgca cgggccgagc cgtggaaagc cgtgccggcc atggcccgca ccgttcgccg
     3121 gctgggcggg catgttctga cccgctgcgc cgtgcgcggc atcgagacgg cggcggggcg
     3181 cgtgtcaggc gtgatcaccg agtcggggcg gattgcctgc caaatggcgg ttctggccgg
     3241 cggcgcctgg tcgcggctgt tcctcggcaa catgggcatc gactttccgc aattgcgcat
     3301 tctcggatcg gtcctgcaga ccgagccgct cgacgggccg cctgtccatg cggtcggcgc
     3361 atccgactat gccttccgca agcgctccga tggcggctac accatcgccc ggcgcggcgc
     3421 cagcgaggcg catatcgtgc cggacagctt ccgtctgttc gtcgatttcc tgccggcgct
     3481 ctacaagcag cggcgcgaga tcaggctggc cctgggcaat cgcttcatgg aggaggcgcg
     3541 cacgccccgg cgctggagcc tggatcaggt gagcccgttc gaaaagacaa ggattctcga
     3601 tcccgagccc agccagaaac tccttgatga agccaggcgc gcggtgatcg ccgcctttcc
     3661 ggcgttcagc ggcatgaaga tcgccaacgc ctggggcggc ctgatcgatg caacgcccga
     3721 tgcggttccg gtcatcggcg aggttccaag gcttgccggc ttcttcatcg ccagcgggtt
     3781 ctccggccat ggcttcggca tcggaccggg cgctgggcgc ctggtcgccg atctggttcg
     3841 cggcgcaacg ccctcggttg atccggcgcc gttccggctg gcacgctttg cgcgtctggc
     3901 gcgcacgacg gcacccggga ttccttgagg acgcgcttgt gaattctcag cttgcggata
     3961 cggtgtaggc tgagaccaat tggtggcggg acgaacccgc ccggaaaaca acggggagag
     4021 acatgtatcg cacgcatctg gctgccggct tggcggcact tgtcatctct gcgccggcga
     4081 tggcgcagaa aaccacactg gttgtcggca tgcagacgca ggatgccggt gtccttgatc
     4141 cgcacctcaa ttccggcacg ccgggcaagg cgctgatgaa ctggatgttc aacggcctgg
     4201 tgcggatcaa gcccggccag gcctcgcccg atcatatcga gcccgacatc gccgaaagct
     4261 ggacctcttc gcccgatggc aaggaatgga ccttcaagct gcgccagggc gtgcaatgtc
     4321 accacgacaa cggcgaattc acctctgagg atgcggtcta ttcgctcaac cgtgccgcaa
     4381 ccaaggacag ctcggcattt tcctccgatt acgcggcctt cgacaaggtc gaggcggtcg
     4441 acaaatacac cgtcaagatc acgctgaagc agcccattcc ctcgctgctg ggccaggtga
     4501 tgagctacca tggcggcaac atggtctgca aaaaggccgc cgaggcgctg ggcaaggacg
     4561 ggttcggcaa gaagccggcc ggcacaggcc cgttcatgtt cgccgaatac gtgccgcagc
     4621 aatatgtgaa gctggtggcc aaccccaagt atttccgtgg tgagccgaag ctcaaggaaa
     4681 tcatctatcg cttcattccg tcggattcag cccgcgatct ggcgttccag gccggcgaaa
     4741 tcgacatgat gatcggccgg caggaacaat cctgggtcga tcgtgtcagc gccacccccg
     4801 ggaccaaggt catcgccttc ggtccggcgg aaatgtctgt gctccacctg aacatgaccc
     4861 agccgccgct cgacaacatc aaggtgcgtc aggcgattgc ccatgccgtc gaccgtgccg
     4921 gcatctggaa tttccgcggc aagaactcgt cgcgccaggc ggtctcggtc gtcccttcag
     4981 gctatgctgg aacgctcgag aatgcgccgc tgctgccgca cgacctggcc aaggccaagg
     5041 ccctgctcgc cgaggcgggt tttcccaacg gtgtgacgat caaggcgatc cacaccacgc
     5101 tgcccggcat gttcaccttt atcgaggccg tgcaggcgca gctcaagcgg gctggcatca
     5161 cgctcgacat cacgccggtc gagcatccga ccttccatca gcagatccgc caggacctgt
     5221 cgcagctcgt gcatttccag gcggcgcgct tcccgatcgc ggatgtttat ctcacccagt
     5281 tcttccactc gcgctcgatc gtcaagacgc cgtcagcggt taccaacttc tcccactgca
     5341 aggtggcgga tgcggagatc gatgcggcgc gcaccgaaca ggacaaggcc aagcagaccg
     5401 cgctctggca tgaggcgcag aagaagatca tcgccgaggt ctgcgcggtt ccggtcaacg
     5461 agatgctgat gatctgggcg ttcaaggaca gccttgacct cggctacgac ctcaaggggt
     5521 cgctcaacct tggtccgccg gtgaccgaac agacccgctt caccaaatga gggctgccac
     5581 ggctttcccc tcccccttgc ggggaggggc cagaggtggg gggcgccgat cgatagccag
     5641 cgttgaactg gcgtttctct tggagcatcc gcccgccagg tggataccgg ctggcggaaa
     5701 aagcggatgc ggaaacaacg agatgtcgga aattagcgat tcagtcagat cggaaagtgc
     5761 tctggaactg ccacccccat ccccaaccct tccccgcaag ggggaagaga gcttgctgcg
     5821 ttactgaccc atgggcggat tcctcctcaa gcgcctcatc ttctcagtgg tcacgctctt
     5881 cgccgtgttg accctggttt ttctgatcgt ccgcgtcgtg ccgggcgatc cggcccaggt
     5941 catccttggc gatcaggcgg atgcggccgc cattgccgcc ttgcgcgggc gccttgggct
     6001 ggatcgcccg gtgctggagc agtatttcag ctttctcggc ggcgcggtga aaggcgattg
     6061 gggcgtctcg ctggtcaccg gccgtccggt catccgcgag gttctcgacg tcctgccctg
     6121 gacgctcgaa ctcacctttg tctcgatggt cctcggcacg gccattggcg tgccgctcgg
     6181 ggtgtgggcg gcgatcaacc gcaaccgaat gccggattat gtaacgcgca tcgcctcgct
     6241 gatcggcctt tccttcccgc ccttcgtctc cgccatcctg ctgctgatca gcttttcgct
     6301 ggtcctgcgc tggtttcctg tcatttcggc caatcgcgcc acgccctggc aatggttcca
     6361 gtccatcgcg cttccggcgc tcaacctcgg cctgatcatg gcggcctata taacgcgcgt
     6421 gacccgttcg gcgatgctgg aggtgatgca ggaggattac gtccggaccg cccgcgccaa
     6481 gggcgcgccg tggcgcgcca tcgtctggcg gcatgcgctg cgcaacgcgc tgatcccgat
     6541 cgtcaccatc gtcgggctct acctcggcat tctgatcggc aattccgtgt tgaccgaaat
     6601 cgtattcaac cggcccgggc tcggcaagct catcatcggc gcgctgagcc agcgcgacta
     6661 caccatgttg cagggcatga tcgtgatcta cacgctcatc gtcgtcctgg tgaacgtcgc
     6721 caccgacctt acctacgcct tcgtggatcc tcgggtgaaa ctcaaatgag cacctcggtc
     6781 catgaaacgt ccaacgccaa ggcttcgcgc acaccgcgtt ggctgaaggc gaccatcgcc
     6841 gccttcaatg ccaacaagac gtcatggata ggcctggtca ttttcgtgct tgtggtgatc
     6901 ctggccctgc ttgcgccgtg gatttctccg cacgatccgg tcgagcagga cgtgctctcc
     6961 aagctcaaac cgccctttgg caactattac ctcggcaccg attactacgg tcgcgacatt
     7021 ctctcgcgcc tgctctatgg cgcgcgcatc agcctcgtca tcggcattgc ctcgacggtt
     7081 ctggcgctgg tgattggctc gatgatcggc attgtcgccg gttattatgg cggacgcatc
     7141 gatgtcgtct tcatgcagac gctggatatg ctgcattcgt ttccctcgct gatcctcggc
     7201 ttgatcctcg tcgccatgct cgggccgacg atgaccaaca tcatcatcgc cattgccctg
     7261 acatcaatcc cctctttcgc tcgcatcgct cgtgcgccaa ccatctcggt caaggagcgc
     7321 gaattcatcg acgcaggacg ggcgctcggc ttctcggata cgcgcctcat ggccgggcat
     7381 atcctgccca atatctttcc tgagattctg gtgatgggct cgctctggct tgccaacgcc
     7441 atccggaccg aagcctcgct cgccttcatc ggcctcggcg tcaagccgcc gacgccgacc
     7501 tggggcggca tgatccgcga ggggttcgag aacattctcg acagctactg gctggtgctg
     7561 gcaccatcga tcgccattct cgtcatcgtc cttgccttgc agattcttgg cgatggcttg
     7621 cgcgacgcga tcgaccccaa gctcaagggc gagcaatgag cggggctgtt ctttccgttc
     7681 gcgatctggt caccgagttt cgtgtcgacg gccagtggcg ccgtgcggtg gacagtgtct
     7741 cgttcgaaat cggcgccggc gagacggtcg ccatcgtcgg cgagtccggc tccggcaaga
     7801 gcgtcaccgc gctgtcggtg atgcggctga tctcgccggt caacggccgt gtatccggtg
     7861 aaatccggct ggaggggcag gaactgctgt gcttgccgga ggatgcgatg cgcgaagtgc
     7921 gcggcaatcg catttcgatg atcttccagg aaccgatgac cagcctcaat ccggtgctga
     7981 cgatcggcca gcagatctgc gaggccctgc aataccaccg tgacatgtcg gcgcgcgagg
     8041 cggaggcgga ggcgatccgc attctcgacc gggtccgcat tcctgcggcg cggcagcgct
     8101 tcggcgaata tccgcaccgg ttttccggcg gcatgcgcca gcgcgtgatg atcgccatgg
     8161 cgctggcctg ccggccgcgt ctgctgattg ccgatgagcc gaccaccgcg ctcgacgtga
     8221 ccatccaggc gcagatcctc gacctgatcc gatcgctgca gcaagaggag gggatgagcg
     8281 tcctgttcat cacccatgac atgggcgtgg tggccgaaat cgccgaccgt gttgtggtga
     8341 tgtggaaggg ccgcaaactt gaggacgagc cggcagtccc gatctttgcc cgcccgcagc
     8401 atggctatac ccgcgctctg ctggcggcag tgcccaagct cggtgaaatg gatgcgcatc
     8461 caaggccgct gcgctttccg gtgatcgagc cgtcgaccgg cgcggttgtg acgccggcca
     8521 gcctggtgcc tgagacggtc cgcccgggag agcccctgct gcaggtgagg gggttgacca
     8581 cgcggttccc gatccgctcc ggcctctttg gcaggatcgg ggggcgggtg cacgccgttg
     8641 aggatgtgac cttcgatctg gccgccggcg agaccctggc gctggtcggc gaatccggct
     8701 gcggcaagtc gacaaccggg cgctcgatcc tgcggctggt cgagccgacc gaggggcagg
     8761 tgcagttcgc cggcctcaat ccgcgcaaga cgatcggcgc ggccatcgcc gagccgctca
     8821 ccgtccacgg ccttgccgac cgcaagggcg cggccgagcg cacggctgaa ctgctgcgcc
     8881 gtgtcggcct gttgcccgac atggcgacac ggttccccca cgaattttcc ggcggccagc
     8941 ggcaacgcat cgccatcgcc cgggcactgg cgctctcgcc cgaactcgtg gtggcggacg
     9001 aggcggtgtc ggcgctggac gtttcggtca aggcgcaggt gctcaacctg atgctggacc
     9061 tgcagcagga actgggcctt gcctatctgt tcatcagcca tgacatggcg gtggtggaac
     9121 gcgtcagcca tcgcgttgcc gtgatgtatc ttggcgaaat cgtcgagatc ggcccgcgcc
     9181 ggcaggttct gtccgatccg catcatccct acacgcgcaa gctgctcgcg gccgtgccgg
     9241 ttcccgatcc gggccggcgg cgcagccggg agccgctggc cgtcgaggaa ctgcccagcc
     9301 cgatccggcc ggccgattgg gtccggccgc aaagcacgct tgtcgaggtt ggaccggacc
     9361 atttcgtgcg ggttgcggcg gggatctgac gcgggccaat tctgccttgc cgcaccccgg
     9421 tcgagcgacc cgccgggatc acgccccctt gggccggaag gccttgcaga tggagggatc
     9481 ggtttccaga cggggcccgc cgatcaggtc gatacaatag ggcacggcag cgaacaccgc
     9541 catcagacag gtgcggatcg cccccggatt gcccggcagg gtgatgatca ggctcttgcc
     9601 gcgcgtgcct gccgactggc gcgacaggat cgcagtcggc acctcattca ggctggcggc
     9661 gcgcatcgcc tcgccgaaac cggccaattc gcgatcatag acggcatgca ccgcttccgg
     9721 cgtcacatcg cgcggggcgg ggccggtgcc gccggtggtc agcaccagcg cggcgccggc
     9781 ctgatcgcag aaggcccgca ccgcggcttc gatcgccgcc cggtcatccg gcacgatgcg
     9841 cgtctcgatc gtgaacggcg aggtcagcac ctccttcagg aaggcttcga tcgccg
//
LOCUS       DAOMXG010000087         9832 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_3648_length_9832_cov_9.043060, whole genome shotgun sequence.
ACCESSION   DAOMXG010000087 DAOMXG010000000
VERSION     DAOMXG010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 9832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9832
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3648_length_9832_cov_9.043060"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(305..877)
                     /locus_tag="PLQ11_07265"
     CDS             complement(305..877)
                     /locus_tag="PLQ11_07265"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018043011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRK24737.1"
                     /translation="MLNRRTFSAAMVGLAVSPALAQAPANPPVTGLDLENTLLMDLKD
                     GRVTIRMRPDLAPRHVAQIKTLVRRGFYNGNVFYRVIAGFMAQTGDPTGQGHVNSDLP
                     NLPAEFSAEPYRRGILGMARHDDPNSANSQFFIMFADGAHLNGKYTVWAEVVSGMEHV
                     DAIKKGDPAKGGRVIEPDRMLKLQVAADAR"
     gene            complement(904..1404)
                     /gene="coaD"
                     /locus_tag="PLQ11_07270"
     CDS             complement(904..1404)
                     /gene="coaD"
                     /locus_tag="PLQ11_07270"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HRK24738.1"
                     /translation="MHSAFYPGSFDPLTNGHMDVLKSTFALVDRLVVAVGIHPGKAPM
                     FSAQERLEMIMACCGPMARAAKVKLEAITFDGLTVDAAKAHGCTILIRGLRDGTDLDY
                     EMQLAGMNQIMAPQVQTVFLPASPLARPITATLVRQISAMGGDVSGFVPQEVLARLQA
                     KKTSRR"
     gene            1556..1831
                     /locus_tag="PLQ11_07275"
     CDS             1556..1831
                     /locus_tag="PLQ11_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24739.1"
                     /translation="MAVALFLFHLRWFGIFAFIALLTIVYGHLNERGVAALSDPAFYS
                     GLVPPLSVAAALAICVPWIMFLIDPRRPAGSLSLEGFARYWKLEQVQ"
     gene            complement(1881..3074)
                     /locus_tag="PLQ11_07280"
     CDS             complement(1881..3074)
                     /locus_tag="PLQ11_07280"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRK24740.1"
                     /translation="MALPLVPLLGLAAVAVSYNQRMLDPLTVLIARDLAVDVTRTIAL
                     SPAFTLPYALGQPFLGPVADSFGKALVLKLCMAVIAITTLAAMFVSDDRLLLALRFVT
                     GLAAGGIIPTCLALIADRTPMAERQLTLSRFMVAIIVGQLFTAPVSAALAEAFGWRAS
                     PLVAIALASLSMLLLLRGLVPRRDAIRPPFSPRRAVALYRQILGLPRARACYVAVAAE
                     GLFVFGFIPHIAVYLSDRGLGGATEAGYILAGMGLGGLVHVLIVRWLVRRFDPFTLMI
                     AGSAMLAVGLVSFALANSWPLMMLSFVILGNGFYLLHTGLQTQVTEILPEARASLVSL
                     HAFWLFVGIAAGPILFGWGMALFGVRATLIASGIAMGLVGTLASRVLRRQVRAFSSEV
                     DAGSR"
     gene            complement(3105..3527)
                     /gene="ndk"
                     /locus_tag="PLQ11_07285"
     CDS             complement(3105..3527)
                     /gene="ndk"
                     /locus_tag="PLQ11_07285"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011926284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HRK24741.1"
                     /translation="MAKQRTFSIIKPDATERNLTGAVNAVIEKAGLRIVAQKRVRMTR
                     KEAETFYGVHRERPFFGELVDFMTSGPVVVQVLEGADAIQRYRDIMGATNPANAAEGT
                     IRKLFARSVGENSVHGSDSAENAAIEIAQFFSGNEIVG"
     gene            complement(3613..5439)
                     /gene="glmS"
                     /locus_tag="PLQ11_07290"
     CDS             complement(3613..5439)
                     /gene="glmS"
                     /locus_tag="PLQ11_07290"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HRK24742.1"
                     /translation="MCGIVGVLGSGPVAHQLVDSLKRLEYRGYDSAGIATLENGHLTR
                     RRAEGKLKNLQARLIEEPLSGHIGIGHTRWATHGRPSEYNAHPHATDRVAVVHNGIIE
                     NFRILREQLLAEGVNFSSDTDTEVVAHLVSRELRKGAGPHQAVAATLRQLHGAFALAF
                     LFEGENDLLIGARLGAPLAVGHGDGEMYLGSDALALAPFTDTIAYLDDGDWVELTRTS
                     ARFFDGDGREVTRAWQKTQAGAFLVDKGNHKHFMAKEIHEQPEVIGRTLAHYLDMAAG
                     RVSLPFALPFDFKSLRKISISACGTAFLAGLTAKYWFERLARLPVEIDIASEFRYREM
                     PLEQGDLSIFISQSGETADTLASLRYAKANGQHILSVVNVPTSTIARESDVVALTLAG
                     PEIGVASTKAFTCQLTVLAALAVAAGVARGTLDAAAEQALVNEMILLPGLVAESLKHE
                     SAIEVVAHDLAKAKDVLYLGRGTSYPLAMEGALKLKEISYIHAEGYAAGELKHGPIAL
                     IEETLPVIVLAPRDQWFDKTVSNVQEVAARGARLLLIGPEGIAREAGLSDADTIAMPE
                     MAPTFAAIAYSVPIQLLAYHTAVTMGKDVDQPRNLAKSVTVE"
     gene            complement(5582..6376)
                     /locus_tag="PLQ11_07295"
     CDS             complement(5582..6376)
                     /locus_tag="PLQ11_07295"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009755728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HRK24743.1"
                     /translation="MSETALRLSLFAGVLAGLVLWELLQPWRDAQQRLRRWPSNFAIV
                     LLDSLIVRLIFPLTAAGVALFAEIKGWGLFALVGAPFWLALPASVILLDLAIYAQHVA
                     FHHVPLLWRFHRMHHADTVLDVSTGLRFHPVEIVLSMLIKFGAVLALGAPPEAVIAFE
                     VLLNATAMFNHANIVLPERLDRLLRLVVVTPAMHRVHHSAIRAETDSNFGFNLPWWDR
                     LFGTYQDGPRLGYERMIVGLDSFRADEEARLDRMLTQPFRRPDKDG"
     gene            complement(6383..7090)
                     /locus_tag="PLQ11_07300"
     CDS             complement(6383..7090)
                     /locus_tag="PLQ11_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HRK24744.1"
                     /translation="MSSLSDFPITRRWPARHPDRIQLYSLPTPNGVKVSIMLEECGLP
                     YEAHAIAIGRNETWTEEFLSLNPNGKIPAIIDPDGPGGKPLGLFESGAILLYLAEKSG
                     QFLPADPARRIETIQWVFFQMAAIGPMFGQLGFFHKFAGREIEDKRPLERYRAETERL
                     LKVLETRLKGRDWIMGDTYTIADIAILGWVRNLIGFYEAGPLVGYERLVEVPRWLARG
                     LERPAVQRGLEIPKRPA"
     gene            7252..8355
                     /locus_tag="PLQ11_07305"
     CDS             7252..8355
                     /locus_tag="PLQ11_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HRK24745.1"
                     /translation="MAKITRIEILQIDLAPKVRRTDAIQSFVTQETPMVRIHTDDGRI
                     GTGYTYTIGTGGSSVIGLLKDHLCPRLIGRDPIHIEMLWKELFFATHATAVGAITSLA
                     LCAIDMALWDLKCLASGRPLWQEAGGAQASVPLYTTEGGWLHIDTSALVEDALKAKAD
                     GFGGSKIKIGRPHVSEDVARLGAVRAAVGPGFEIMTDANQAFTVSEAIRRARAYEGLD
                     LAWFEEPVPAEDLGGHVRLCQATSIPIAVGESIYHPSHFREYLQRGACSIVQADVARV
                     GGITPWLKIAHLAETFNVSICPHFLMEIHVSLCAAVPNAAWVEWIPQLDDVTTSRMGV
                     KEGRAVPPSAPGIGIDWDWASIERLAPPRHSLT"
     gene            8367..8714
                     /gene="arsC"
                     /locus_tag="PLQ11_07310"
     CDS             8367..8714
                     /gene="arsC"
                     /locus_tag="PLQ11_07310"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018687668.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HRK24746.1"
                     /translation="MRATIYHNPSCGTSRTVLAQLRVAGYQPIVVEYLKTGWTQPQLE
                     ALFAAAGITPRQALRTSNSPAEVMGLTAPGISDATILAAMVREPVLVNRPIVVTDKGT
                     RLCRPAGLLEEIL"
     gene            8800..9771
                     /locus_tag="PLQ11_07315"
     CDS             8800..9771
                     /locus_tag="PLQ11_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013761518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid TRAP transporter substrate-binding
                     protein SiaP"
                     /protein_id="HRK24747.1"
                     /translation="MLKTTLTAATVIAGVLLSTSSMAQTKLKWAHVYETSEAFHTESV
                     WAAGEIAKRTNGRYTIDVFPASQLGKETDINQGLALGSVDIIISGSSFAARSYPPIGV
                     TYYPFTFRDADHLMAYTKSDVFKDLTKGYEDKTGHKIVAVTYYGVRHTSSNRAIKTCA
                     DMKGLKMRVPDVPAYLAMPRACGANTAPIAFAEVYLALQNGTVEAQENPLTTIEAKKF
                     YEVQKHIVLTGHIVDHLNTVISGNLWKKLSPEDQKVFAEVAQEAAARATAQIKKREAE
                     LVAFFKEKGLTVTEINRKEFLDASLASAKFETYGYNKADWDKIQAIK"
BASE COUNT         1908 a         3218 c         3028 g         1678 t
ORIGIN      
        1 cgggcgcctg ctgcgccgga gccggctggg cctgggcgat ggcggcagtg gtcagggcca
       61 caagcggcag ggcgatgagc aggcggcgca gcatggcggt gtccttccgg caggagaatc
      121 agttcatacg tccgctgact ttataccaat tcgagtcaaa agcgcgcgcc ccatgtgctc
      181 ctctgcacat ccagagcatt ttcaagcgaa gtggagtccg gctcgcgtca agaaaatgca
      241 agaaaacaaa aagatagagc gctttttgtg aattggaatg cgctgaaagt gctccagaaa
      301 gaattcagcg cgcatccgcc gccacttgca gtttcagcat ccgatccggc tcgatcaccc
      361 ggccaccttt ggccggatcg cccttcttga tcgcgtcgac atgctccatt ccggaaacaa
      421 cttcggccca gacggtatac ttgccgttga gatgggcgcc gtcggcaaac atgatgaaga
      481 actgcgaatt ggcggaattg ggatcgtcat gccgggccat gccgagaatg ccacggcgat
      541 agggttctgc cgaaaactcg gccggaagat tcggcaggtc ggaattgaca tggccctgcc
      601 cggtcggatc gcccgtctgc gccatgaaac cggcgatgac ccggtagaat acattgccgt
      661 tgtaaaatcc gcgacggacc agcgtcttga tctgtgccac atgcctgggc gcaagatcag
      721 gccgcatgcg gatggtgacg cggccgtcct tcagatccat cagcagcgtg ttttcgaggt
      781 ccagcccggt caccggcggg tttgcggggg cctgggccag cgcgggagaa acggccaatc
      841 ccaccatggc ggcagaaaaa gtgcggcggt tcagcatggc atgctccgaa attggtagaa
      901 tgatcagcgg cgtgacgttt tcttcgcctg aagccgggca agcacctcct gcggcacaaa
      961 gcccgacaca tcgcccccca tcgccgagat ctgccgcacc agcgttgccg tgatcgggcg
     1021 cgccaacggg gacgccggca gaaacacggt ctgcacctgc ggcgccatga tctggttcat
     1081 cccggcaagc tgcatttcgt aatcgagatc ggtgccgtcg cgcaggccac ggatcagtat
     1141 ggtgcagcca tgcgccttgg cggcatccac cgtcagcccg tcgaaggtga tcgcctcaag
     1201 cttgaccttg gcggcccggg ccatcgggcc gcagcaggcc atgatcatct ccagccgctc
     1261 ctgagcgctg aacatcggcg ccttgccggg gtggataccg accgcgacaa ccagtcggtc
     1321 aaccagcgca aaggtgctct tgagcacatc catatgcccg ttggtcaacg gatcgaacga
     1381 cccgggatag aaggcactgt gcatgaaatc cccccgtttg ccggcctgca agagactgag
     1441 agccagggtc aggacctagc agaccggggg cgatggcggc aatgccgacc aaggatgagc
     1501 gggacaacct gcttgcgcgc cgctataatc cgcttcctgt cgttgagagg tctgaatggc
     1561 tgttgccctg ttcctgtttc atctgcgctg gttcggcatc tttgccttca tcgcgctcct
     1621 gaccatcgtc tatggccatc tgaacgagcg cggcgtggcg gccctgtccg atccggcctt
     1681 ctacagcgga ttggtgccac cgttgagcgt tgctgcggca ctggcgatct gcgtgccctg
     1741 gatcatgttc ctgatcgatc cgcgacgccc ggcgggcagc ctgtcacttg aaggctttgc
     1801 gcgctactgg aagctggagc aggttcagtg aattccagtt cacgtaaaag tgctctaacc
     1861 ttctgttttg tcgcattttc ttaacgcgaa ccggcatcca cttcgcttga aaatgctcta
     1921 acctgacgtc gcagcacccg gctcgccagc gtgcccacaa ggcccatggc gattcccgag
     1981 gcgatcagcg tcgctcggac accgaacagg gccatccccc agccgaacag gatcggcccg
     2041 gcggcaatgc cgacaaacag ccagaaggca tgcagcgaca ccagcgaggc acgcgcctcg
     2101 ggcaggatct cggtcacctg ggtctgcaac ccggtatgca gcaggtaaaa gccattgccg
     2161 aggatgacaa agctcagcat catcagcggc cagctgttgg ccagcgcaaa cgacaccagc
     2221 ccgacagcca gcatcgccga gccggcgatc atcagcgtga acggatcaaa gcgccgcacc
     2281 agccagcgca cgatcagcac atggacaagc ccgccaagcc ccatgcccgc caggatgtag
     2341 ccggcctcgg tcgcgccccc gagaccgcga tcgctgaggt agaccgcgat atgcggaatg
     2401 aagccgaaca cgaacagtcc ttccgccgcc accgccacat agcaggcgcg ggcccgcggc
     2461 aggccgagga tctgtctgta gagtgccacc gcgcggcggg gagagaacgg cgggcggatc
     2521 gcatcccgcc tcggaaccag cccccgcagc agcagcagca tcgacaggct ggccagtgca
     2581 atcgccacca gcggcgaggc ccgccagcca aaagcctccg ccagagcggc agagaccggc
     2641 gcggtgaaca attgcccgac gatgatcgcc accatgaacc ggctgagcgt caattgccgc
     2701 tcggccatcg gcgtgcggtc ggcaatcagc gccaggcagg tggggatgat gcccccagcc
     2761 gccagcccgg tgacaaaccg caaggccagc agcaaccggt cgtcactgac aaacatcgcc
     2821 gccaatgtgg tgatggcgat gaccgccatg cagagcttga gcaccagcgc cttgccgaaa
     2881 ctgtcggcga ccggccccag gaatggctgg cccagcgcat agggcagcgt gaaggccggg
     2941 gaaagcgcga tggtgcgcgt aacgtcgacc gccagatcgc gggcgatcag caccgtcagc
     3001 ggatcgagca tccgctggtt ataggaaacc gcgacagcgg ccagtccgag aaggggaacc
     3061 agcggcaggg ccatgagcgc atcctgccgc cgtctgccgc tcgatcagcc gacaatctcg
     3121 ttgccggaaa agaactgggc aatctcgatg gccgcattct cagccgaatc cgaaccatgc
     3181 accgaattct cgccgaccga gcgggcaaac agcttgcgga tcgtgccctc ggcggcattg
     3241 gccgggttgg tggcgcccat gatgtcgcga tagcgctgga tcgcgtcggc accttccagc
     3301 acctgcacga ccaccggacc ggaggtcatg aaatcgacca gttcgccgaa gaacgggcgc
     3361 tcgcgatgca caccgtaaaa ggtctcggcc tccttgcggg tcatgcgcac gcgcttctgg
     3421 gcgacaatgc gcagtccggc cttctcgatc acggcattga cggcgccggt gaggttgcgt
     3481 tcggtcgcgt ccggcttgat gatggagaag gtgcgctgct tggccatggt ccagtcctga
     3541 tgatcgggtt gagtgttggc acgcctctta ctgcctcgcc agcccttaga aaaggggctt
     3601 tgcgcggcgg cgctattcca cggtgaccga ttttgccagg ttgcggggct ggtcgacgtc
     3661 tttgcccatc gtcaccgctg tgtgataggc gagcaactgg atcggaaccg aataggcgat
     3721 ggcggcaaag gtcggcgcca tctccggcat ggcgatggtg tccgcgtccg acaatcccgc
     3781 ttcgcgggcg attccctccg gtccgatcag cagtagccgg gcaccgcgcg cggccacctc
     3841 ctggacattg gaaaccgtct tgtcgaacca ctggtcacgc ggggcaagca cgatcaccgg
     3901 cagggtttcc tcgatcagcg cgatgggccc gtgcttgagt tcgcccgccg cataaccttc
     3961 ggcatggatg tagctgattt ccttcagctt caacgcgcct tccatggcca gcggatagga
     4021 ggtgccgcgc cccagataga gcacatcctt ggccttggcc agatcgtgcg ccacgacttc
     4081 gatggcgctt tcatgcttga gcgattcggc gaccagaccg ggcagcagga tcatctcgtt
     4141 gaccagcgcc tgttcggctg cggcatccag cgtgccgcgg gcaacgccgg ccgcaaccgc
     4201 cagcgctgcc agcaccgtca gctggcaggt gaacgccttg gtggaggcga cgccgatttc
     4261 cggccccgcc agcgtgagag ccacaacgtc gctctcgcgg gcgatggtcg atgtcggcac
     4321 attcaccacc gagagaatgt gctggccatt ggccttggca tagcgcagcg aggccagggt
     4381 atcggccgtc tcgcccgact gcgagatgaa gatcgacaga tccccctgct ccagcggcat
     4441 ttcacgataa cggaattcgg aggcgatatc gatctcgacc ggcaggcgcg caagccgctc
     4501 gaaccagtac ttcgccgtca acccggcaag gaacgccgtg ccgcaggcgg aaatggaaat
     4561 cttgcgtaag gacttgaaat cgaacggcag cgcgaagggc aggctcaccc tgccggcggc
     4621 catgtccaga taatgggcca gcgtccgtcc gatcacttcc ggttgttcgt ggatctcctt
     4681 ggccatgaag tgcttgtggt tgcccttgtc gaccaggaag gcgccggcct gggttttctg
     4741 ccaggcacgg gtgacctcgc gaccgtcgcc atcaaaaaat cgcgcactgg tgcgcgtcag
     4801 ttccacccag tcgccatcat cgagataggc gatggtgtcg gtgaacgggg caagcgccag
     4861 cgcgtccgag ccgagataca tctcgccatc gccgtgaccg accgccagcg gagcgccgag
     4921 gcgcgccccg atcagcagat cgttttcgcc ctcgaacagg aaggcgagcg cgaaagcgcc
     4981 atgcaattgc cgcagcgttg ccgcgaccgc ctgatgcgga ccggcgccct tgcgcaattc
     5041 acggctgacc agatgggcga ccacctcggt atcggtatcg gacgagaaat tgaccccctc
     5101 ggcgagcagc tgctcgcgca ggatgcggaa attctcgatg atcccgttat gcacaaccgc
     5161 gacgcgatcg gtcgcatgcg gatgggcgtt gtattcgctc ggccgcccgt gggttgccca
     5221 gcgggtgtgg ccgatgccga tatgcccgct caagggttcc tcaatcagcc gggcctggag
     5281 gttcttgagc ttgccctcgg cgcggcgccg ggtcaggtgg ccgttctcca gcgtcgcaat
     5341 gccggcagaa tcgtatccgc gatattccag gcgcttgagc gaatcgacca gctgatgggc
     5401 gaccggtcca gaccccagca ccccgacaat tccgcacata cctgtctcca aatgattctt
     5461 cgttccttca gcaggcaagc cttgtgccgt ccatgcccgc ttggctcaag tcactttaga
     5521 caacaagcgt aagatttgag ctttttggct gtgcggcccg gcgaaacggc cttccgtgcc
     5581 gctatccatc cttgtccggc ctgcggaatg gctgggtcag catccggtcg agccgcgctt
     5641 cctcgtcggc gcggaagctg tccagtccca cgatcatccg ctcgtaaccg agccttggcc
     5701 catcctgata cgtcccgaac agcctgtccc accagggcag gttgaagccg aaattgctat
     5761 ccgtttcggc ccggatcgcc gaatggtgga cgcggtgcat cgccggcgtg accacgacca
     5821 gccgcagcag gcggtccagc cgctcgggca gcacgatatt ggcatggttg aacatcgccg
     5881 tggcgttgag cagcacctcg aaggcgatca ccgcctcggg tggtgccccc agcgccagca
     5941 ccgcgccgaa cttgatcagc atcgacagca caatctcgac cggatgaaac ctcagcccgg
     6001 tcgaaacgtc gaggacagtg tcggcatgat gcatccggtg aaaccgccac agcagcggca
     6061 catgatggaa ggccacatgc tgggcgtaga tggcaaggtc gagcaggatg accgaggccg
     6121 gaagtgccag ccagaacggt gcgccgacca aggcaaacaa cccccagccc ttgatctcgg
     6181 cgaacaatgc cacccctgcc gccgtcagcg gaaagatcag ccgcacgatc aggctgtcga
     6241 gcaggacgat ggcgaaattg gacggccagc gccgcaagcg ttgctgcgca tcgcgccagg
     6301 gttgcagcag ttcccacagc accagcccgg ccagcacgcc ggcaaacagg gagagacgaa
     6361 gcgcggtctc gctcatcgcc ggtcaggccg gacgcttggg tatttcgagc ccgcgctgca
     6421 ccgccgggcg ttccagcccg cgcgccagcc agcgcggcac ctcgaccagc ctttcatagc
     6481 cgaccagcgg cccggcctcg taaaagccga tcagattgcg cacccagccc aggatcgcga
     6541 tgtcggcgat ggtataggtg tcgcccatga tccagtcgcg ccccttcaac cgcgtctcca
     6601 gaaccttgag caaccgttcg gtctcggccc gatagcgctc cagcgggcgc ttgtcttcga
     6661 tctctcggcc ggcgaatttg tgaaaaaacc cgagctggcc gaacatcggg ccgatcgcgg
     6721 ccatctggaa gaacacccac tggatcgtct cgatccgccg cgccggatcg gccggcagga
     6781 actgtccgga cttttcagcg agatagagga ggatcgcacc cgactcgaac aggcccagcg
     6841 gcttgccgcc cggcccgtcc ggatcgatga tcgccgggat cttgccgttg gggttgagcg
     6901 acaggaattc ctcggtccag gtctcgttcc tgccgatcgc gatggcatgc gcctcgtagg
     6961 gcagcccgca ttcctccagc atgatcgaca ccttcacccc gttcggggtt ggcagcgaat
     7021 agagctggat gcggtcggga tggcgggccg gccagcggcg ggtgatcgga aaatcggaca
     7081 gggaggacat ggggcgactc gtcagccaat ggggtgatgc gatggtcgcc caggttaccg
     7141 gtcaccggca agtcaatttg acgcgacacc ccgcacgcct gccttggtgc ttttccaatc
     7201 gcgccgggcg cgctaccgat gagggcccct gtttccagac cggatcccgc catggccaag
     7261 atcacccgca tcgaaatcct gcagatcgac cttgcgccga aggtcaggcg caccgacgcc
     7321 atccagtcgt tcgtcacgca ggaaacaccg atggtgcgca tccacaccga cgacggacgc
     7381 atcggcaccg gctacaccta taccatcggc accggcggtt cgtcggtcat cggcctgttg
     7441 aaggaccacc tctgcccgag gctgatcggc cgcgacccta tccacatcga gatgctgtgg
     7501 aaggagctgt tctttgccac ccacgccacg gcggtcggcg ccatcacctc gctggcgctc
     7561 tgcgccatcg acatggcgct gtgggatctc aaatgcctcg cctcgggccg cccgctgtgg
     7621 caggaggccg gcggcgcgca ggcctcggtg ccgctctaca ccaccgaggg cggctggctg
     7681 cacatcgaca cgtccgcgct ggtcgaggat gcgctgaagg ccaaggcgga cgggtttggc
     7741 ggctcgaaga tcaagatcgg tcggccgcat gtgtcggagg atgtagcgcg gctcggtgcg
     7801 gtgcgcgctg cggtcggtcc cgggttcgag atcatgaccg atgccaatca ggcgttcacg
     7861 gtcagcgagg cgatccgtcg cgcccgggcc tacgaagggc tcgatctggc ctggttcgag
     7921 gagcccgtcc ccgccgagga tcttggcggc catgtccggc tgtgccaggc gacctcgatc
     7981 ccgatcgcgg tgggtgaatc gatctaccat ccgagccact tccgcgaata tctccagcgc
     8041 ggcgcctgtt cgatcgtgca ggcggatgtc gcccgcgtcg gcggaattac cccatggctg
     8101 aagatcgccc atctggccga gaccttcaat gtctcgatct gtccgcattt cctgatggaa
     8161 atccacgtct cgctctgtgc agctgttcca aatgccgcat gggtggaatg gataccccag
     8221 cttgatgacg tgaccaccag ccgcatgggt gtcaaggaag gtcgcgccgt gccgccgtcc
     8281 gcgcccggca tcggcataga ctgggattgg gcgtcgatcg agcgtctggc gccgccccgc
     8341 cacagcctga cctgaccggt gataccatgc gcgcaacgat ctatcacaac ccttcctgcg
     8401 gcacctcgcg caccgtgctg gcgcaactgc gcgtggccgg ctaccagccg atcgttgtcg
     8461 agtatctcaa gaccggctgg acacagccgc aacttgaagc gttgtttgcc gcggccggga
     8521 ttacaccgcg ccaggcgctg cgcaccagca attcgccggc cgaggtgatg ggactgaccg
     8581 cgcccggcat cagcgacgcg accatcctcg ccgcgatggt ccgcgagccg gttctggtca
     8641 atcgcccgat tgtcgtcacc gacaagggca cccgcctctg ccggcccgcc gggctgctgg
     8701 aagaaattct ctgacccgtc gatagacatc atacaacgct tgacactcag cagcagatgc
     8761 ggcgggatgc acccacaaca aacatcgggg agtgccaaaa tgctcaagac gaccctcact
     8821 gctgcgaccg tgattgccgg tgtgctgctt tccacctcgt ccatggcgca gaccaagctg
     8881 aaatgggcgc atgtctacga gacgtccgaa gcattccata ccgaatccgt ctgggctgcc
     8941 ggcgagatcg ccaagcgcac caacggccgc tacacgatcg acgtgttccc ggcctcgcag
     9001 ctcggcaagg agaccgacat caaccagggc ctggcgctcg gctcggtcga tatcatcatt
     9061 tccggatcga gctttgccgc ccgctcctat ccgccgatcg gcgtcaccta ttatcccttc
     9121 accttccgcg atgccgacca cctgatggcc tacaccaagt cggatgtgtt caaggacctg
     9181 accaagggct acgaggacaa gaccggccac aagatcgttg cggtaaccta ttacggtgtt
     9241 cgccacacct catccaaccg cgccatcaag acctgcgccg acatgaaagg cctcaagatg
     9301 cgcgtgccgg atgtgccggc ctacctcgcc atgccgcgcg cctgcggcgc caacaccgcg
     9361 ccgatcgcct tcgccgaggt ctatctcgcc ctgcagaacg gcacggtcga ggctcaggaa
     9421 aacccgctga ccacgatcga ggccaagaag ttctacgaag tccagaagca catcgtgctg
     9481 accggccaca tcgtcgatca tctcaacacg gtgatttccg gcaacctgtg gaagaagctg
     9541 tcgcccgagg atcagaaggt ctttgccgaa gtggcccagg aagcagcggc ccgcgccacc
     9601 gcgcagatca agaagcgtga ggctgaattg gtcgccttct tcaaggaaaa ggggctgacc
     9661 gtgaccgaga tcaaccgcaa ggagttcctc gacgcctcgc tcgccagcgc caagttcgag
     9721 acctacggct acaacaaggc cgactgggac aagatccagg cgatcaagtg acccactgac
     9781 atcgcgacat cccctctccg cctgcaggat aggggggccg ggccgacgcg tg
//
LOCUS       DAOMXG010000088         9828 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_3651_length_9828_cov_12.200143, whole genome shotgun sequence.
ACCESSION   DAOMXG010000088 DAOMXG010000000
VERSION     DAOMXG010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 9828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9828
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3651_length_9828_cov_12.200143"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..377)
                     /locus_tag="PLQ11_07320"
     CDS             complement(66..377)
                     /locus_tag="PLQ11_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003601785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HRK24748.1"
                     /translation="MMGMMKQVQAMQAKFAEMQAELEQLEVEGSSGGGMVKVVVLAKG
                     ALKSIRIDPSLMQPDEAEILEDLIVAACNDARGKAEALLAEKMKALTGGLPLPPGLKL
                     F"
     gene            complement(409..2187)
                     /locus_tag="PLQ11_07325"
     CDS             complement(409..2187)
                     /locus_tag="PLQ11_07325"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012453721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HRK24749.1"
                     /translation="MAQTPETGGAEAPASAYRVLARKYRPRDFSDLIGQEPMVRTLRN
                     AFAANRIHQAYIFTGVRGVGKTTTARIIARGLNYVAADGSGGPTVDLAGYGRHCEAIL
                     ESRHVDVIEMDAASHTGINDIREIIDSVQYRPVDARYKVFIIDEVHMLSTQAFNGLLK
                     TLEEPPPHVKFLFATTEIRKVPVTVLSRCQRFDLRRIVAADMVTYLKAIAGKEGFEIS
                     DEALALIARASEGSMRDALSLLDQAIAHADGRIEAERLRTMLGLADRSRVIDLFEAVM
                     RGDLAASLTELKSQYDSGADPAGIIADLADYVHVVTRLKILPGDANDPTLSESERTRG
                     QGFAEALSQKVLTRAWQILLKGLAEVQTAARPVSAAEMLLVRLAYAADLPTPDEALRR
                     LREAAREDSGSPISARPAPAASPLPRLPAGNTLPQPAPTLRQDVAPAAEPAPSLRIRR
                     FEDIPAIALERRDLALKVAVERDLRLVSFEEGRIEVNVVGPSGDVVTRLARALQTWTG
                     SRWIVAVSQQSGASTLAEQEQARRADDMRGVRANPVVRAILEDFPGAEIVAVRLLRGE
                     TDSSAMTDEIDGQAAPPVEADDVDDL"
     gene            complement(2461..2557)
                     /gene="ffs"
                     /locus_tag="PLQ11_07330"
     ncRNA           complement(2461..2557)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLQ11_07330"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            2644..3078
                     /locus_tag="PLQ11_07335"
     CDS             2644..3078
                     /locus_tag="PLQ11_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HRK24750.1"
                     /translation="MSDFRLDPRLERDTLVLGDLALCRVLMMNDARYPWLILVPRRPD
                     LTELIDLIPADQESLFREIVEVSAALRAVHNPDKLNIGALGNIVRQLHVHIVGRFASD
                     PAWPGPVWGHSPAHPYPAHMAGIARDRLIAALDPYGLRDGTT"
     gene            3075..4028
                     /gene="nudC"
                     /locus_tag="PLQ11_07340"
     CDS             3075..4028
                     /gene="nudC"
                     /locus_tag="PLQ11_07340"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HRK24751.1"
                     /translation="MSRFVSLNERYAPEDLGFAHGRLDRAGDRRDDGRFLTGALASGN
                     AGIYALVGETVVLKTTDPLGALFSPAELPLAEARREQVFLGLDGDAPRFGVLLEDTAA
                     ERLAAIPGLALISLRQCAIEGLLPPDQLGALATAKALLHWHQTHRFCARCGAPSHLRQ
                     AGWRRDCDNCGAQHFPRTDPVVIMLAIRGQSCLLGRQPRFLPGMYSCLAGFCEPGETI
                     EDAVRREIKEEAGIEIGRVRYLASQPWPFPQSLMIGCIAEAQSETITRDVTELEDARW
                     FTRAEVLAMMARKHPEAVHVPAKFSLASQLVQAWAVEGIGL"
     gene            4153..4866
                     /locus_tag="PLQ11_07345"
     CDS             4153..4866
                     /locus_tag="PLQ11_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HRK24752.1"
                     /translation="MTPIMPDLFAKTDTWVFDLDNTLYPPHSDLWPKIDHRITVFLAH
                     LLDLDGLSARALQKHYYQRYGTTLNGLMAEHADIINADRFLDFVHDIDRTTLMIDDRL
                     GAAIERLPGRRLVFTNGSRGHAEKTMLALGIAHHFDAVFDIVAGQFRPKPDPVTYERF
                     LTAHDIDPSRAVMFEDIARNLEPPKHLGMRTVLVVPRAGGTDHRDPWEQANTAPSYID
                     HVTDDLAGFLDAVAGGLGL"
     gene            4955..5821
                     /gene="dapD"
                     /locus_tag="PLQ11_07350"
     CDS             4955..5821
                     /gene="dapD"
                     /locus_tag="PLQ11_07350"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HRK24753.1"
                     /translation="MLTAQLNAAIERAWENRAEISPATRGEAREAVEAALDALDAGRL
                     RVAEKTPGESGRSAWKVNEWLKKAVLLSFRLNDMDIVPFGNATGGTHYGWDKVRLKTA
                     GWSAAEFRAAGFRAVPGAIVRRSAFVARNVVLMPSFVNLGAYVDEGTMVDTWATVGSC
                     AQIGKNVHLSGGVGIGGVLEPLQASPTIIEDNCFVGARSEVVEGVVVGEGSVIAMGVF
                     ISASTRIVDRATGTVHVGYVPPYSVVVSGNLPGKPLPDGTPGPSTYCAVIVKTVDAQT
                     RSKTGINDLLRE"
     gene            complement(5873..7090)
                     /gene="rmuC"
                     /locus_tag="PLQ11_07355"
     CDS             complement(5873..7090)
                     /gene="rmuC"
                     /locus_tag="PLQ11_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020179395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HRK24754.1"
                     /translation="MQDPFVIVAGHTITLQDALIYGALLALGLLCVLVWLQARMGNAR
                     AIEAATAAERAREMDEKMAALNQLQAEMTGRIQGFAEVLGSRQTDLARLVSERLDSVK
                     SSVGQGLTQQAERTADTLQKLNERLAVIDAAQTSLAGLTSEMLGLKDILANKQARGAY
                     GQGRMEAIVRDGLPPSAFAFQTTLSNRSRPDCVIHLPGDTRPLVVDAKFPLESFSLLR
                     AAQSDEARATAERRVRNDVGLHVKDIAEKYLITGETQDLALLFVPSEAVYADLSEHFE
                     DLVQRAHKARVLIVSPSLLMMAIQVMQAIVRDARMRDQARVIQTEVGRLLDDVRRLQE
                     RASKLDQHFRQAQDDVAGLIVSSDKVSKRGERIEALDFEEAPAAKAPPPVEPKLPLGA
                     DVLPSPLLRRAAR"
     gene            7199..7714
                     /gene="def"
                     /locus_tag="PLQ11_07360"
     CDS             7199..7714
                     /gene="def"
                     /locus_tag="PLQ11_07360"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003583874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRK24755.1"
                     /translation="MTIRPLVILPDAQLRKVSEPVRAIDSSLQRLADDMLETMYDAPG
                     IGLAAIQVGAPVRLITIDLSKKTEEEKSPQVFINPEIVWSSPETSSYEEGCLSIPDYY
                     ADVSRPARVKVRYRDLAGRDQEVLAEGLLATCIQHEIDHLNGVLFIDHISRLKRTMVM
                     KKFTKMARDRA"
     gene            7758..8690
                     /gene="fmt"
                     /locus_tag="PLQ11_07365"
     CDS             7758..8690
                     /gene="fmt"
                     /locus_tag="PLQ11_07365"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HRK24756.1"
                     /translation="MTLRIVFMGTPDFSVPVLTEILGQGHEVVGVYTRPPAPARRGME
                     MQKSPVHQAADRFNLPVFTPKSLKKAAAQAEFAALDADVAVVVAYGLILPKAVLEAPR
                     HGCLNLHASLLPRWRGAAPIQRAIMAGDAETGVMVMQMDEGLDTGPVAMVEKIVISPA
                     MTAGELHDRLMRLGADLMARALAALERGALTIRPQMETGVTYAAKLTNDEGRIDWSKT
                     AQEVHNQIRGLSPYPGAFLEADWSQGRQRLKVLRSEITEGAGTAGTVLDSRLTIACGS
                     GAVRLLEVQRAGKGPMQAADFLNGARIGPGLRLA"
     gene            8690..9433
                     /gene="truA"
                     /locus_tag="PLQ11_07370"
     CDS             8690..9433
                     /gene="truA"
                     /locus_tag="PLQ11_07370"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HRK24757.1"
                     /translation="MPRYKVILEYDGAPYAGWQLQAGWRTVQGDLEEAVRQFAGENAR
                     VNGCGRTDAGVHALAQVAHIDLTRDWRTDTVRDALNAKLKLIDARVAVLHAEHVDERF
                     DARFSARRRHYLYRILNRRPPSPLLADYTWHVPRKLDVTAMNAAATVLVGHHDFSTFR
                     AANCQARSPEKTLEFLHVEHRGDEVEVATHARSFLHHQVRSMVGSLVEVGLGRWSRED
                     LERARDARRRDACGPTAPAHGLYFVKVDY"
     gene            complement(9518..>9828)
                     /locus_tag="PLQ11_07375"
     CDS             complement(9518..>9828)
                     /locus_tag="PLQ11_07375"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HRK24758.1"
                     /translation="ANWMIPGKMVKGMGGAMDLVAGVQKVVVVMEHEAKGESKLMHRC
                     NLPLTGASVVDMVITDLGVFTIDKKGKGGMTLIELADGVTLDEIKAKTGATYKVGLKG
                     "
BASE COUNT         1776 a         3155 c         3076 g         1821 t
ORIGIN      
        1 gatgcgcctc cagcacctcg gggcgttcgc gcagggtgtg caggaacttg cgctggcagt
       61 cgggctcaga acagcttgag gccgggcggc agcggcaggc ccccggtcag ggccttcatt
      121 ttctccgcca gcagggcttc agccttgccg cgcgcatcgt tgcaggcggc gacgatcagg
      181 tcctcaagga tctccgcttc gtccggttgc atcagcgagg ggtcgatcct gatgctcttc
      241 agcgcgcctt tcgccaggac cacaaccttg accatgccgc cgccggacga accttcaact
      301 tcgagttgtt cgagctcggc ctgcatctcg gcgaatttcg cctgcatggc ctgcacctgc
      361 ttcatcatgc ccatcatggc tgttgttccc gtgttggaag gaagggggtc agaggtcgtc
      421 aacgtcatcg gcctcgaccg gaggggccgc ctgcccgtcg atctcatctg tcattgcact
      481 gctgtcggtc tccccccgta gcaggcgcac cgcgacgatc tcggcgcccg ggaagtcctc
      541 gagaatggcc cgcaccaccg gattggcccg aacgccgcgc atgtcgtcag cacgacgggc
      601 ctgttcctgt tcggcaagcg tcgaagcgcc cgactgctgg ctgaccgcga caatccagcg
      661 gctgccggtc caggtctgca gggcgcgggc aaggcgtgtg accacatcac cggacgggcc
      721 gacgacattg acctcgatcc gcccttcttc gaacgaaacc agcctgagat cgcgttcgac
      781 ggccactttc agcgccagat cacggcgctc aagcgcaatg gccggaatat cctcaaaccg
      841 gcggatacgc aggctgggcg cgggttcggc agcgggcgca acgtcttggc gcagagttgg
      901 cgccggttgc ggcagggtgt tgccggccgg cagacgcggc aggggggaag cggctggcgc
      961 aggtctggcg ctgatcggcg aacctgaatc ctcccgcgca gcctcgcgca ggcggcgcaa
     1021 cgcctcatcc ggtgtcggca ggtcggcggc ataggccaga cgcaccagca gcatctcggc
     1081 cgcagaaacc ggtcgggcgg ccgtctgcac ctcggccaac cccttgagca ggatctgcca
     1141 agcgcgggtc agcaccttct gcgacagcgc ttcggcgaac ccctgcccgc gcgtgcgttc
     1201 gctctcgctc agcgtcggat cattggcgtc gcccggcaga atcttgagcc gggtgacgac
     1261 atgcacatag tcggccagat cggcgatgat tccggctgga tcggcgccgg aatcatattg
     1321 ggacttgagc tcggtgagcg aagcggcaag atcgcccctc atcaccgcct cgaacaggtc
     1381 gatgacgcgg ctgcggtcgg ccaatcccag catggtcctg agccgctcgg cctcgatacg
     1441 gccatcggca tgggcaatcg cctgatcgag cagcgacaag gcatcgcgca tcgaaccttc
     1501 ggaggcgcgc gcgatcagtg ccagcgcctc atcggaaatc tcgaaaccct ccttgccggc
     1561 gatagccttg aggtaggtca ccatatcggc ggcaacgatg cgcctgaggt caaatcgctg
     1621 gcagcgcgac agcaccgtga ccgggacctt gcggatctcg gtggtggcga acaggaattt
     1681 cacatgcgga ggcggctctt ccagcgtctt gagcaggccg ttgaaggcct gcgtcgaaag
     1741 catgtgcacc tcatcgatga tgaagacctt gtagcgcgca tcaaccgggc gatactgcac
     1801 cgaatcgatg atctcgcgga tatcgttgat gccggtatgc gaggcggcgt ccatctcgat
     1861 cacgtcgaca tgccggcttt ccagaatcgc ctcgcaatgc cggccatagc cggcaagatc
     1921 gaccgttggt ccgccgctgc cgtcggccgc cacataattg aggccgcggg caatgatacg
     1981 cgccgtggtc gtcttgccga caccgcgcac ccccgtgaag atataggcct gatggatacg
     2041 gtttgcggca aaggcgttgc gcagcgtgcg caccatcggc tcctggccga tcaggtcgga
     2101 aaaatcgcgc gggcgatact tgcgcgccag cacccgatag gcagaagcag gcgcctcagc
     2161 gccgccggtt tcaggtgttt gagccatcag cgcatcaggt gccgggatga ccggcaggtg
     2221 gcaagggtca acagaccaat tcttccgtgt tttgaccgac tcgccaaacc acggaaaaac
     2281 atgatcgata acaatacatt agagcatgct tgaagcaaaa agccgtttcc actttcttgc
     2341 agcatgccct tagaacaaaa gccgatctga ctggatcgct cttttgctct aacctattct
     2401 gaacccatca ccttttccgc cagccaggta tccacctggc gggatgatgc tctcaggaag
     2461 attgggaggc tggacaacga cccgcccgta ctcgttaggg ctgcttcctt ccggacctga
     2521 cccggttggc gagtggtacg tccacagcca acctcccgct ccctatatcg ggcaacttcc
     2581 ttgcaaaaac aagcatggcg tcaaaggctg gttttggata acgcttggag aaaaggaagg
     2641 cgcatgtccg attttcgtct cgacccaagg ctggagcgcg acacattggt tctgggagac
     2701 ctcgcgctct gccgcgtgct gatgatgaac gatgcgcgct acccctggct gatcctggtg
     2761 ccgcggcggc cggatttgac cgaactgatc gatcttatcc ctgcggatca ggaatcgctg
     2821 ttcagggaaa tcgtggaggt cagcgcggca ctgcgcgccg tgcacaaccc cgacaagctc
     2881 aacatcggtg cgcttggcaa catcgtccgc cagctgcatg tgcatatcgt cggccggttt
     2941 gcctctgacc ccgcatggcc cggcccggtc tggggccaca gcccggcgca cccctaccct
     3001 gcacatatgg ccggtattgc acgtgaccgt ctcattgccg cgctagaccc gtatgggttg
     3061 agggacggca ccacatgagc cggtttgtgt ccctcaacga gcgttatgcc cccgaagatc
     3121 tcggctttgc ccatggacgg ctcgaccggg caggcgaccg gcgggacgat ggccgctttc
     3181 tgaccggtgc cctggcgagt ggcaatgctg gcatttacgc gctcgtcggt gagacagtcg
     3241 tcctgaaaac gaccgatccg cttggggcgc ttttttctcc cgccgaactg ccactggccg
     3301 aggcccggcg ggaacaggtg tttcttggcc tcgacgggga cgcgccgcgt tttggcgtgc
     3361 tgcttgagga caccgctgcc gaacggctgg cggcgattcc aggccttgcg ctcatcagcc
     3421 tgcgccaatg tgccatcgaa ggcctgctgc cgcccgacca actcggtgcg ctggcaacgg
     3481 caaaggcact gctccactgg catcagaccc atcgcttctg tgctcgctgc ggggcgccct
     3541 cgcacctgcg gcaagccggc tggcggcgcg attgcgacaa ttgcggcgcc cagcacttcc
     3601 cgcgcaccga tcctgtggtg atcatgctgg cgatacgcgg tcaatcctgc ctgctgggtc
     3661 gccaaccgcg ctttcttccg ggcatgtatt cctgccttgc cggtttctgc gagccgggtg
     3721 agacgatcga ggatgccgtg cggcgggaga tcaaggaaga ggccgggatc gagatcggcc
     3781 gcgtgcgcta tctggccagc caaccctggc cgtttccaca gtcgctgatg atcggctgca
     3841 tcgccgaggc acaaagcgag accatcaccc gggatgtgac cgaacttgag gatgcccgct
     3901 ggttcacccg tgccgaagtg ctcgccatga tggcacgcaa gcatcccgag gctgtccatg
     3961 tgccggcaaa attttcgctg gcctcccagc tggtgcaggc ctgggcagtc gagggaatcg
     4021 gcctctgaac gagggctgac acggttttgc aagacttgag cgctagggtc cggagctatg
     4081 ttcgacagtt ccagcctcca tgcactgccc gttgaagcgg ttcctgccag cgcccgcgaa
     4141 aggctcggtg caatgacccc gatcatgccc gatctcttcg ccaagaccga cacctgggtt
     4201 ttcgatctcg acaacacgct ctatccgcct cattccgatc tctggccgaa gatcgaccac
     4261 cgcatcacgg tctttctggc ccatctgctt gacctcgacg ggctctcggc ccgcgcgctg
     4321 caaaagcact actaccagcg ctatggcacc accctgaacg ggctgatggc cgaacacgcc
     4381 gacatcatca atgcggatcg ctttctcgat ttcgtgcatg acatcgaccg caccacgctg
     4441 atgatcgacg accgtcttgg ggcagccatc gagcggctcc ccggccggcg cctggtattt
     4501 accaatggct cgcgtggcca tgcggaaaag accatgctgg cgcttggtat cgcgcaccat
     4561 ttcgacgctg ttttcgacat tgtcgcaggc caattccgcc ccaagccgga tcctgtcacc
     4621 tacgagcggt ttctgactgc gcatgatatc gatccaagtc gcgccgtcat gttcgaggat
     4681 atcgcgcgca atctcgaacc gcccaagcat ctcggcatgc gcaccgtgct ggtggtgccc
     4741 agggcgggcg gcacggatca tcgtgatccc tgggagcagg ccaatacagc gccctcctac
     4801 atcgatcatg tcactgatga ccttgccggc tttctggacg ctgtggccgg cggtctcggc
     4861 ctgtaaaatc cggccaggta tgtgccttcg cgcttgactt ggcgcccgcc tttctggcga
     4921 aagggggcat ctggttttgc tttcgggatc gatcatgctc accgcccaac tgaacgccgc
     4981 aatcgaacga gcctgggaaa accgggcgga gatttccccc gccacaaggg gggaggcccg
     5041 cgaggccgtc gaggcggcat tggacgcgct tgatgcggga cggcttcgcg tcgccgaaaa
     5101 gaccccaggg gaaagcggcc gcagcgcctg gaaggtcaac gaatggctga agaaggcggt
     5161 gctgctgtcg ttccgcctga atgacatgga tattgttccg ttcggaaatg cgaccggcgg
     5221 cacccactac ggctgggaca aggtgcgcct caagacggct ggctggagcg ctgcggagtt
     5281 tcgcgctgca ggtttccgcg cggtgccggg cgccatcgtg cgccgctcgg ccttcgttgc
     5341 gcgcaatgtc gttctgatgc cgtccttcgt caacctcggg gcctatgtcg acgagggcac
     5401 catggtcgat acctgggcaa ccgtcggttc ctgtgcccag atcggcaaga atgttcatct
     5461 gtcaggcggc gtcggcatcg gcggtgtgct ggaaccgttg caggccagcc ccaccatcat
     5521 tgaggacaat tgctttgtcg gcgcaagatc cgaagtggtc gagggcgtcg tggtcggcga
     5581 agggtcggtg atcgccatgg gcgtgttcat ttccgcatca acccggatcg tcgatcgcgc
     5641 gaccggcaca gtccatgtcg gctatgtgcc gccctattcg gtggtggtct ccggcaatct
     5701 gcccggcaag ccgttgcctg acggcacgcc cggcccctcc acctactgcg ccgtgatcgt
     5761 caagacagtg gacgctcaga cccgctccaa gaccggaatc aacgaccttc tgcgcgaata
     5821 gcgcaagagc gcacaacggc aggcagcgcg ttgctgcggc ggcagggatc catcaccgcg
     5881 ctgctcgccg caacagcggc gaaggcaaga catcggcccc cagcggcagc ttcggttcga
     5941 caggcggcgg ggccttggcc gccggcgcct cctcgaaatc aagggcctcg atccgttcgc
     6001 cgcgcttgct taccttgtcc gacgagacaa tcagacccgc aacatcgtcc tgcgcctgac
     6061 ggaaatgctg gtccagtttg ctcgcccgct cctgcagacg ccgcacatcg tccagcaggc
     6121 gaccgacctc ggtttgaatc acccgggcct ggtcccgcat gcgcgcatcg cgcacgatag
     6181 cctgcatcac ctgaatcgcc atcatcagca gcgagggcga gacgatcaga acccgtgcct
     6241 tgtgggcgcg ctggaccaga tcctcgaagt gctcgctgag atcggcgtag accgcctcgg
     6301 acggcacgaa cagcagcgcc agatcctgtg tctcgccggt gatgaggtat ttttcagcaa
     6361 tgtccttcac atgaaggcca acatcgttgc gcacgcgccg ttcggcggtt gcgcgcgcct
     6421 cgtcgctctg ggccgcccgc aacagagaga aggactcgag cggaaatttc gcatcgacca
     6481 ccagcggccg ggtatcgccc ggcagatgga tgacgcaatc gggccgcgaa cggttggata
     6541 aagtggtctg gaaggcaaag gccgaaggcg gcaaaccatc gcggacgatc gcctccatcc
     6601 gcccctgacc ataggcgccg cgcgcctgct tgttggcgag gatgtccttg aggccgagca
     6661 tctcgctggt cagcccggca aggctggtct gggctgcatc gatcaccgcc agccgctcat
     6721 tgagcttctg cagcgtatcg gcggtgcgct cggcctgctg ggtcagaccc tggccgacgc
     6781 tggatttgac cgagtcgagc cgctcggaaa cgaggcgggc aagatcggtc tggcgcgagc
     6841 cgagcacttc ggcaaagccc tggatgcgcc cggtcatctc cgcctgaagc tggttgagcg
     6901 cagccatctt ctcgtccatc tcgcgcgccc gttcggcggc ggtcgcagcc tcgatggcgc
     6961 gggcattgcc catgcgcgcc tgcaaccaga caagcacgca cagcagcccc agcgccagca
     7021 atgcgccata gatgagcgca tcctggaggg taatcgtatg gccggcgacg atcacaaacg
     7081 ggtcttgcat gcgctatagc tagcgcgatt cggcgcgaac gaaaatagaa cgcattgacc
     7141 ccccggtggc cacgctctat ggaaggccga atttgttcca tccgacagac caaaccccat
     7201 gaccatccgt ccgctcgtca ttcttcccga tgcacagctg cgcaaagtgt ccgaaccggt
     7261 ccgggccatc gattccagcc tccagcgcct ggccgatgac atgctggaga cgatgtatga
     7321 tgcccccggc atcggccttg cggccattca ggtgggcgcg ccggtgcggc tgatcaccat
     7381 cgacctttcc aaaaagaccg aggaagaaaa aagccctcag gtttttatca atcccgagat
     7441 cgtctggtcc tcgccggaaa cctcgtccta cgaggaaggc tgcctgtcga ttcccgacta
     7501 ttatgccgat gtcagccggc cggcccgggt gaaggtccgc tatcgcgacc ttgcaggcag
     7561 ggaccaggaa gtgctcgccg aagggctgct tgccacctgc atccagcatg agatcgacca
     7621 tctcaacggc gttctgttca tcgatcacat ctcgcggctc aagcggacca tggtgatgaa
     7681 gaaattcacc aagatggccc gggaccgggc atgagggaac cgatggcggg gataccggcc
     7741 gacggatggg caaatcgatg acccttcgca tcgttttcat gggcacgccc gatttttccg
     7801 tgccggtgct gaccgaaatt ctcggccagg ggcatgaagt tgtcggcgtc tacacccgtc
     7861 cgccggcccc ggcccggcgc ggaatggaga tgcagaaaag tccggtgcat caggcggcag
     7921 accggttcaa cctgccggtg ttcacgccca aatcgctgaa gaaggccgcc gcgcaagccg
     7981 agtttgccgc gctcgacgcc gatgtggcgg tcgtggtcgc ctatgggctc atcctgccga
     8041 aggcggttct cgaggcgccg cgtcatggct gtctcaacct gcatgcctcg ctgctgccgc
     8101 gctggcgggg cgcggcgccg atccagcgcg cgatcatggc gggcgatgcc gagaccggcg
     8161 tcatggtcat gcagatggac gaagggcttg ataccggtcc ggtcgcgatg gtggaaaaaa
     8221 tcgtcatcag tccggcgatg accgccggcg aattgcacga taggctgatg cggcttggcg
     8281 ccgaccttat ggcgcgggcg ctggcagcgc tcgagcgcgg ggccttgacc atccgcccac
     8341 agatggagac tggcgtcacc tacgccgcca agctgaccaa cgacgagggc cgcatcgact
     8401 ggtcgaagac cgcgcaggag gtccataacc agattcgggg actctccccc tatccgggcg
     8461 cctttctcga ggccgattgg agccagggac gccagcggct caaggttctg cgttctgaga
     8521 tcaccgaagg cgccgggaca gccggaacgg tgctcgatag ccggcttact attgcgtgcg
     8581 gctcgggcgc ggtacgcctt ctcgaggtgc aacgagccgg aaaggggccg atgcaggctg
     8641 ccgacttcct caacggagcg cgaatcggac cgggactcag gctcgcctga tgccacgcta
     8701 caaggtcatc ctcgaatacg acggcgcccc ctatgccggt tggcagttgc aggcgggttg
     8761 gcgcacggtg cagggcgacc tcgaagaagc cgtcaggcag ttcgccggag agaatgcgcg
     8821 cgtcaacggt tgtggacgga ccgatgccgg ggtgcatgcg ctggcacagg tcgcccatat
     8881 cgacctgacc agggactggc ggaccgatac cgtgcgcgat gcgctcaatg cgaagctgaa
     8941 gctgatcgat gcgcgcgttg cggtcctgca tgcagagcac gtggacgagc gtttcgatgc
     9001 acggttttcg gcgcggcggc gccactatct ctaccgaatc ctcaatcgtc ggccgccaag
     9061 cccgctcctg gccgactata cctggcatgt gccgagaaag ctggacgtta cggcgatgaa
     9121 cgccgcagcg accgttcttg tcgggcacca tgatttttcg acgttccgcg cggccaattg
     9181 ccaggcgcgg agtccggaaa agacactcga attccttcat gtcgagcatc ggggcgacga
     9241 ggttgaggtt gccacccatg cccggtcatt cctgcatcat caggtgcgct cgatggtggg
     9301 ctcactggtg gaggtggggc ttggtcgctg gagccgggag gaccttgagc gggcgcgcga
     9361 tgcgcgtcga cgcgatgcct gtggtccgac ggcgccggca cacgggttgt attttgtcaa
     9421 agtggactat tgaggggcgg cagcgctgtc agtcccggat tgcccttgca aggcgcccgg
     9481 gactgacagc ggccacgtcc gttcagccca agcctgttca gcccttgagc ccgaccttgt
     9541 aggtggcccc ggtcttggcc ttgatctcgt cgagcgtcac gccatcggca agttcgatca
     9601 gtgtcatgcc gcccttgccc ttcttgtcga tggtgaagac accgagatcg gtgatcacca
     9661 tgtcgacgac actggcgccg gtcagcggca ggttgcaacg atgcatgagc ttggattccc
     9721 ccttcgcttc atgctccatg accacgacga ccttctgcac accggccacg agatccatcg
     9781 cgccgcccat gcccttgacc atcttgccgg ggatcatcca gttggcga
//
LOCUS       DAOMXG010000089         9759 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_3681_length_9759_cov_9.430647, whole genome shotgun sequence.
ACCESSION   DAOMXG010000089 DAOMXG010000000
VERSION     DAOMXG010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 9759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9759
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3681_length_9759_cov_9.430647"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="PLQ11_07380"
     CDS             <1..480
                     /locus_tag="PLQ11_07380"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRK24759.1"
                     /translation="ASSPPMLMFSSEGEKDSVRNVRETGEFCCNIVSHELMHPMNATS
                     APLPRDTSEFGPAQLTPIASTLIRPPRVKEARAALECRLVSITRLMTLSGVPSNNFLV
                     IGQVVGVHIDDGLLRDGKVDAQALKPLARLGYKDYAAIDRVFELGRPPGGGDLEKVK"
     gene            complement(589..738)
                     /locus_tag="PLQ11_07385"
     CDS             complement(589..738)
                     /locus_tag="PLQ11_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24760.1"
                     /translation="MKRLVNLVIATAFAASLAACATPPQAQDDLTPPARKLDAKTQLE
                     TGRTR"
     gene            873..2165
                     /locus_tag="PLQ11_07390"
     CDS             873..2165
                     /locus_tag="PLQ11_07390"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HRK24761.1"
                     /translation="MTPAARIAAAIEVLAEIDSRRRPVGEVLAEWGRAHRFAGSGDRA
                     DVANLAYDALRVQSSARWLMGEETPRATVIGMLKRLRGLDAGQIAALFGADRYAAPAL
                     TDAETARLATATLEGAPAPVRGDYPDWLDPLFERAFGQARVAEIAALAARAPLDLRVN
                     ALKAVRAQIMQAIGHLAPEPTPISPLGLRLAPRDNGRVPYVQAEPAFIKGAVEVQDEG
                     SQLVAAIARARPGEQVLDLCAGAGGKTLALAAAMNNSGQIFATDNDARRLRNIHERLE
                     RAGVRNVQVRTPTARAPKVEGLSGRMDLVLVDAPCTGTGTWRRNPDAKWRIRPGSLEQ
                     RVKEQGEVLDIAAPLVKPGGRLAYITCSLLDEENGAQVSAFLARHRQFSVVSPAEAAA
                     AAGLGVLSGHSDCTGQGLLLSPARTGTDGFFVALLMRQ"
     gene            complement(2169..2855)
                     /locus_tag="PLQ11_07395"
                     /pseudo
     CDS             complement(2169..2855)
                     /locus_tag="PLQ11_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928570.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF599 domain-containing protein"
     gene            2958..3374
                     /locus_tag="PLQ11_07400"
     CDS             2958..3374
                     /locus_tag="PLQ11_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRK24762.1"
                     /translation="MAQTITKGIKALLSEANSQVRTLSVAEAMALHGSPGVVFVDLRD
                     PRELDREGRLPGAFHCPRGMLEFWIDPESPYAKPVFQQDRQFVFFCAGGWRSALAAKA
                     ARDMGLKPVAHVEGGFGAWKKAGAPVELPEKAPGDT"
     gene            complement(3460..4311)
                     /locus_tag="PLQ11_07405"
     CDS             complement(3460..4311)
                     /locus_tag="PLQ11_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRK24763.1"
                     /translation="MKLVRYGRAGHEKPGLVDGDGKIRDLSSHIDDLAGEALSPRSLA
                     RIARLKPERLPPVRGRPRLGPCVGAVGNFVAVGLNYADHARETGAKIPGEPILFNKAP
                     SCISGPNDDVVLPMGSQKTDWEVEIAVVIGTRASYVSEAEALDHVAGYCICNDVSERA
                     FQAERGGQWMKGKGCATFGPLGPWLVTRDEIADEQNLPMWLDVNGERMQTGSTATMIF
                     PIRMLVSYISQFMILNPGDVITTGTPPGVGLGMKPPRFLKPGDIMRLGIQGLGEQRQL
                     VSAFRKV"
     gene            4604..7024
                     /locus_tag="PLQ11_07410"
     CDS             4604..7024
                     /locus_tag="PLQ11_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24764.1"
                     /translation="MSESRREPGSLGGGGLLGQIRDTTVVPARLWTRRRLVLVMLVFA
                     AAWASFGMPWLSGRVTIPYDAKAHFQAQLQFLAQSLHNGQSPFWTPNVFGGSPQIADP
                     QSLIFSPAILLAGLVPNPSIRALDSFVLGLLGLGGLAVLMFFRDRGWHPAGGIVAGLA
                     FAFGGAAAWRIQHIGQVMSFAQFGITLWLLSRALDRPSVLYGMLAGASAALMVVKPDQ
                     VGLLSVYILAGYVVCHWLSAPDRKAALVASLKPLLAGAVTATLLAGIPLLMTIMFADA
                     SQRAEIAYSEAVRGSLHPGSLLTGLVGDLFGAGNARVDYWGPSSSTWSPGVLTLSQNM
                     GEIYFGILPIVAILTIGLVRGLVLAREIRFFTIALGLMLIYALGMYTPGFRLMYEFLP
                     GVSAFRRPADATFLIGGLGAIVGGYLVHCWLTHRFHLPNPWRLAIKGAVLTLSFALAF
                     GVAAYFNRLAEAAWPIGIAVLLAASGLGLVKLLQAHAQRFPVLAAAALAAFMVVDLGL
                     NNGPNESTGLPPKDYEMMDPATRNSTIQLLKQKLAQGSGPAQRDRVELLGVGFEWPNL
                     GLVHRFDHVLGYNPLRLKDFVEATGAQDTIAGPDQRVFTPLFPSYGSLFADMIGLRYI
                     ASSVPIEQIDKRLQPGDLKFLARTEDAYVYENTGALPRALFVRGWKIADFDDLMETGR
                     WPKFNPTTEVLLEEAPELPASSRTTGLALVNLRSYTNTRIEIDVETPDAGFVVLNDVW
                     HKWWRATVDGEEADILKANVLFRAVQIPPGRHTIVYEFRPLEGMMAELREKIAGEDGE
                     AETIVNIP"
     gene            7133..8452
                     /locus_tag="PLQ11_07415"
     CDS             7133..8452
                     /locus_tag="PLQ11_07415"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020811547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HRK24765.1"
                     /translation="MRIAMVGSGYVGLVSAACFADFGHTVVCVDKDEGKIAGLRDGRV
                     PIFEPGLANMVKSNAAEGRLVFTTSLAEAMAEAAIVFIAVGTPTLRGDGHADLSYVYA
                     AAREIAGLISGFTVIVTKSTVPVGTGEEVERIIREARPDAEFAVVSNPEFLREGAAIG
                     DFKRPDRIIIGTSDERARAVMEEVYRPLHLNEGPLVFMGRRDAELTKYAGNAFLAMKI
                     TFINEMADLAEAVGANVQAVARGIGLDKRIGAKFLNPGPGYGGSCFPKDTLALVKTAR
                     DAGRPVRLIETVVDINDQRKAAMVDKIVAASGGSVAGKRIAILGLAFKPNTDDMRDAP
                     ALVIVPKLKTLGADVVAFDPEAHEQAERLMPGLAFAKGPYDCIAGADAVVIVTEWDAF
                     RALDLARVRSLVRTPVMVDLRNIYRVEQVRAHGFAYSSIGRPDHPGG"
     gene            complement(8471..9469)
                     /locus_tag="PLQ11_07420"
     CDS             complement(8471..9469)
                     /locus_tag="PLQ11_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRK24766.1"
                     /translation="MHVLVTGSAGFIGFHVAERLLQGGHSVTGIDGFTDYYDVRLKER
                     RAALLANHRRYSGHRLRLEDRTATLAIAEAAKPDIVIHLAAQAGVRYSLENPATYLDC
                     NITGLFSVMEAARAAGAKHLMFASTSSVYGAPRTTPFRETQAADRPLSFYAATKKAGE
                     ALLHAYSHAWNLPTTAFRFFTVYGPWGRPDMAPQKFIEAIEAGRGIDVYNHGQMRRDF
                     TYIDDIVEAILRLSGTPPVVGQPAGPMDTLSPDAPFRIVNIGRGAPVELMDFIDSLER
                     ALGKTAEKRFLPMQPGDVPETYAATDLLSALTGFTPSVSLDDGLRRLVDWYRANPL"
BASE COUNT         1658 a         3123 c         3184 g         1794 t
ORIGIN      
        1 gcgagcagcc cgccgatgct gatgttttca tccgaaggcg aaaaggattc ggtgcgtaat
       61 gtgcgcgaga ccggggaatt ctgctgcaac atcgtcagtc atgagctgat gcatccgatg
      121 aacgcgacct cggcgccgct gccgcgtgac acctccgaat tcggcccggc acagcttacg
      181 cccatcgcct cgacgctgat ccgcccgccg cgtgtgaagg aggcgcgcgc cgcgctcgaa
      241 tgccggctcg tctcgatcac ccggctgatg acgctctccg gcgtgccgag caacaatttt
      301 ctggtgatcg ggcaggtggt cggcgttcac atcgacgacg gattgctccg cgacggcaag
      361 gtcgatgcgc aggcgctgaa gccgctggcc cgcctcggct acaaggatta tgcggccatc
      421 gacagggtgt tcgaactcgg ccgcccgccc ggcggcggcg atcttgaaaa ggtaaagtaa
      481 gccgcagaac cgtccttccc cgcagcagca aggcactacc gggaaaaaga caaacccgcc
      541 ggggcggtca tgctgcccgg cgggtctgaa agccgaaagc cgcagaattt agcgggtgcg
      601 gccggtctcg agctgggtct tggcgtcgag cttgcgggcc ggcggggtga gatcgtcctg
      661 ggcctgcggc ggcgttgcgc aggcggcgag cgaggcggcg aaggcggtcg cgatgaccag
      721 attgacgagg cgcttcatgg cgatgttcct tgatgtggga cgcgaaaaca ggttccgtct
      781 ttgcctgaaa taaggcaagg acgtggccaa tcggtaaatt tgtcagggat gacgcaacgt
      841 ttttgtctcc ttgccggcct tgaggaccct tgatgacccc cgctgcccgc atagccgccg
      901 ccatcgaggt gctggccgag atcgattccc gccgccgccc ggttggcgag gttctggctg
      961 aatgggggcg ggcgcatcgc tttgccggct ccggtgaccg cgccgatgtc gccaaccttg
     1021 cctacgacgc attgcgcgtc cagtcctcgg cccgctggct gatgggcgag gaaaccccgc
     1081 gcgcgaccgt gatcggcatg ctcaaacggc tgcgcggtct cgatgccggt cagatcgccg
     1141 cattgttcgg cgccgatcgc tatgccgcgc cggccttgac cgacgccgag acggcgcggt
     1201 tggcaacggc cacgctggaa ggtgccccgg cgccggtccg cggcgattat cccgactggc
     1261 tcgatccgtt attcgaacgc gcattcggcc aggcgcgggt tgcggagatc gcggcgctgg
     1321 cggcccgcgc gccgctcgat ctgcgcgtca atgcgctcaa ggcggtccgc gcacagatca
     1381 tgcaggccat cggccatctc gccccggaac cgacgccgat ttccccgctc ggccttcgcc
     1441 tggccccgcg cgacaacggg cgggttccct atgtccaggc cgagccggcc tttatcaagg
     1501 gcgcggtcga ggtgcaggat gagggctcgc aactggttgc cgccatcgcc cgggcacggc
     1561 ccggcgaaca ggtgcttgat ctttgcgccg gcgccggcgg caagacgctg gcgctcgccg
     1621 ccgcgatgaa caacagcggc cagatcttcg cgaccgataa cgatgcccgc cggctcagga
     1681 acatccacga gcggctcgag cgggccggcg tccgcaatgt tcaggtgcgc acgccgaccg
     1741 cccgcgcgcc gaaagtggag gggctctccg ggcgcatgga tctggtgctt gtcgatgccc
     1801 cctgcaccgg caccggcacc tggcggcgca atccggacgc caaatggcgt atccgtcccg
     1861 gctcgctgga gcagcgggtc aaggaacagg gcgaagtgct ggacatcgcc gctccgctgg
     1921 tcaagcccgg cgggcgcctt gcctatatca cctgctcgct gctcgatgag gagaacggcg
     1981 cacaggtcag cgcctttctg gcgcggcacc ggcagttctc ggttgtttcc ccggctgaag
     2041 cggcggcggc ggcggggctt ggcgttcttt ccggacattc cgactgcacc gggcaggggc
     2101 tgttgctcag cccggcccgc accggcaccg acgggttctt cgttgccttg ctgatgcggc
     2161 aatgacgctc atttcggatc gagaatcgag ctcagcgcat ccgacccgta ttgccgccgc
     2221 gccatgacga acagtgccgc caccgtggac gccagaaaca ccagcggccc gaggaaccag
     2281 ccgagatagg ccagagcgaa gaagaacgcc cgctggccgc ggttgaaatg ccgcccggcg
     2341 gcaaccgtca tgtcgccgac gcggcgggcc tgcaactcgg cctcggcatc gatgcgcgat
     2401 gcggttggcg tcgcccccat caggatcgcc gcttagttga acagccggta ggcccaggca
     2461 aacttgaaaa acgcatagac gaagatcacc gccagcccca ccaccttcaa ttcccacaac
     2521 gcggcatacg gcacggcgcc gaatggcagg ctggcaagca gggcggtaag gtccgagccg
     2581 gcacggatca gcgacagcga gccgccgacc gcaatcagcg aggtcgaggc gaaaaacgcc
     2641 gacccctgat gcagcgccgc catcacctgc acatcgacca tccggttatc gcgtcgcagc
     2701 atctcgctca tccagcgctg ccgataggca ttcatcctgg cattgaggct gttgccgccg
     2761 aaagtcctgg tctcgatgag gaaatgatag ctcccccagc acaccagaaa aacgctgatg
     2821 gcgatcatgt cgatctgcgt gaaaccctga atcatgtccg ttgcccgccg ctgtgcttgc
     2881 ctcgcgcggc ggtttgatcg catgatcggg cgcgtgaggg aaccgccatg ttggaatggc
     2941 gcagcatgag gacaggcatg gcgcagacga tcaccaaggg aataaaagcc ctgctttcgg
     3001 aggcgaacag tcaggttcgc acgctgtcgg ttgccgaggc catggccctg catggcagcc
     3061 ccggcgtcgt ctttgtcgat ctgcgcgatc cccgcgaact ggacagggaa gggcggcttc
     3121 ccggcgcctt ccattgcccg cgcggcatgc tggaattctg gatcgacccg gaaagccctt
     3181 acgccaagcc ggttttccag caggacaggc agttcgtgtt cttctgcgcc ggcggctggc
     3241 gctcggcgct ggcggcaaag gcggcgcggg atatgggcct gaaacccgtc gcccatgtcg
     3301 aaggcgggtt cggcgcctgg aagaaggccg gcgcaccggt ggagctgcct gagaaggcgc
     3361 caggagacac ctgacccgtc cgatgcgggc ggtccgaggg tgaccagccg gtcaggcccg
     3421 ggcgctacgg atggggatcc cggccgccga tcacgccaat cacactttgc ggaaggcgga
     3481 gaccagctgg cgctgctcgc cgaggccctg gatacccagg cgcatgatat cgcccggctt
     3541 gagaaagcgg ggcggcttca tgccgaggcc gacgcccggc ggcgtgccgg tggttatgac
     3601 atcgcccggg ttcaggatca tgaactgcga gatgtaggag accagcatcc ggatcggaaa
     3661 gatcatcgtc gcggtcgagc cggtctgcat ccgctcgcca ttgacatcga gccacatcgg
     3721 caggttctgc tcatcggcga tttcgtcgcg cgtcaccagc caaggcccca gcgggccgaa
     3781 ggtggcgcac cccttgccct tcatccactg gccgccccgt tcggcctgga acgcgcgctc
     3841 agacacatca ttgcagatgc agtagcctgc gacgtgatcg agcgcttcgg cctcgctgac
     3901 gtagctggcg cgggtgccga tcaccacggc aatctcgacc tcccagtcag tcttctgcga
     3961 tcccatcggc agcacgacat catcgttcgg tccgctgatg caggagggcg ccttgttgaa
     4021 caggatcggc tcgccgggga ttttggcgcc ggtctccctg gcatggtcgg cgtagttgag
     4081 cccgaccgcg acgaaattgc cgacagcccc gacacagggt ccgagccgcg gccgcccgcg
     4141 caccggcggc agccgctccg gcttaagccg ggcaatgcgc gccagacttc tcggcgaaag
     4201 agcctcgccg gcaagatcgt cgatatggct ggacaagtca cgaattttgc catcgccatc
     4261 cacgagccct ggcttttcat ggccggctct gccgtaacga accaatttca cgcccgcctc
     4321 ccgctgccca tttcaggtta taacttgcta tcaaatggcc cccgaatgcc gctcttatac
     4381 tcacctattg aaaaagatga caagcaagtg agccacaatc ccggccaaat cgcaacgacc
     4441 ggagacggat tttgtcacgc atcactgaat ggtgaaccat cgggagaatt attttcttcg
     4501 ttgctttaac cgcaatggcg caacgacgct ctgtcggggc atacaggcct tgttcaggtg
     4561 gcgtgcgcga cagtatgagc cgagcggtct ctgggatcga agcatgagcg aaagccggcg
     4621 tgagccgggc tcgcttggag ggggcgggtt gttggggcag atcagggaca ccacggttgt
     4681 tccggcgcgt ctttggacgc ggcgtcgcct tgtgctcgtg atgctggtgt ttgcggctgc
     4741 ctgggcctcg ttcggcatgc cgtggctgtc gggccgcgtc accattccct acgatgccaa
     4801 ggcgcatttc caggcgcaat tgcagttcct ggcccaatcg ctgcacaatg gccagtcgcc
     4861 gttctggacg cccaatgtct tcggcggctc gccgcagatc gccgatccgc aatcgctgat
     4921 cttctcgccg gccattctgc tggctgggct ggtgccgaat ccgagcatcc gtgcgctgga
     4981 ttcgtttgtt ctggggctgc ttggcctcgg cggcctggct gtcctgatgt tcttcaggga
     5041 tcgcggctgg catccggccg gcggcatcgt cgccgggctg gcctttgcct tcggcggggc
     5101 ggcggcctgg cgcatccagc atatcgggca ggtgatgagc tttgctcagt ttggcatcac
     5161 gctttggctg ctgtcacgcg cgctggatcg cccgtcggtg ctctacggca tgctggccgg
     5221 cgcttcggcg gcgctgatgg tggtcaagcc cgatcaggtc ggtctgctgt ccgtctatat
     5281 tctggcgggt tacgtcgtct gccactggtt gagcgctccc gaccggaaag cggctcttgt
     5341 tgccagcctc aagccattgc ttgccggagc tgtcacggcg acgctgctgg cgggcatccc
     5401 gctgttgatg acgatcatgt ttgctgatgc ctcgcagcgc gctgagatcg cctattccga
     5461 agcggtgcgc ggctcgctcc atcccggatc gctgctgacc ggactggtcg gcgacctgtt
     5521 cggcgccggc aacgcccggg tcgactactg gggaccgtcg agcagcacct ggagccctgg
     5581 cgtgctgacc ctgtcgcaga acatgggcga gatctacttc ggcattctgc cgattgtcgc
     5641 gatcctgacc atcggcctgg ttcgcggact ggttctggcg cgcgagatcc gctttttcac
     5701 catcgctctg gggctgatgc tgatctacgc gctcggcatg tatacgcccg gtttccggct
     5761 gatgtatgaa ttcctgccgg gcgtcagtgc cttccgccgt ccggccgatg caacctttct
     5821 gatcggcggc cttggcgcga ttgtcggcgg ctatctggtg cattgctggc tgacgcaccg
     5881 gttccacctg ccaaacccct ggcggctggc gatcaagggc gcggttctga ccctgagctt
     5941 cgcactggcg tttggcgttg ccgcctattt caaccggctt gccgaagcgg cctggccgat
     6001 cggcatcgcg gtcctgctgg ccgcctcagg cctggggctg gtcaagcttc tgcaggccca
     6061 tgcccagcgc tttccggtgt tggctgcggc ggcgctggcg gccttcatgg tggtggatct
     6121 cgggctgaac aacggcccca acgaatcgac gggtctgccg ccgaaagact atgagatgat
     6181 ggatccggcg acccgtaaca gcaccattca gctgctcaag cagaagctgg cgcaaggcag
     6241 cggtcctgcc cagcgcgacc gtgtcgaact gctcggcgtc ggctttgaat ggcccaatct
     6301 cggcctcgtt caccgcttcg atcatgtgct gggctacaac cctctccggc tgaaggattt
     6361 cgtcgaggcg acgggcgcgc aggatacgat tgccggcccg gaccagcgcg tgttcacacc
     6421 gctgtttccc tcctacggct cgctgttcgc cgacatgatc gggctgcgtt acatcgcctc
     6481 gtcggtgccg atcgagcaga tcgacaagcg attgcagccg ggtgacctta aattccttgc
     6541 caggaccgag gatgcctacg tctacgagaa taccggagcg ctgccgcgcg ccctgttcgt
     6601 gcgtggctgg aagatcgccg atttcgatga cctgatggaa accggcaggt ggccgaaatt
     6661 caaccccacc accgaagtgc tgcttgaaga ggcgccggaa ttgcccgcca gcagccgcac
     6721 caccggcctg gcgctggtca acctgcgctc ctataccaat acccgcatcg agatcgacgt
     6781 cgagacgcct gacgccggct ttgtcgtgct caatgatgtc tggcacaaat ggtggcgggc
     6841 gactgtcgac ggcgaggagg ccgatatcct caaggccaat gtgctgtttc gcgctgtcca
     6901 gattccgccc ggtcgccaca ccatcgtcta cgaattccgc ccgctggaag gcatgatggc
     6961 cgagttgcgc gaaaagatcg ctggcgagga tggcgaggcc gagacaatcg tcaacattcc
     7021 ctgatgcatc gcgccgtctg cccggtcgcg ctgtccgcgg ccgtggcctg ttccaaacgt
     7081 taaggtctat gttctaacgt ggcgccggtt tttgcagcgg agactgggac tcatgcgcat
     7141 agcgatggtc ggttcgggat atgtcgggct ggtatcggcg gcctgttttg ccgattttgg
     7201 ccatacggta gtctgtgtcg acaaggacga gggcaagatc gccggtctgc gcgatgggcg
     7261 cgtcccgatt tttgagccgg gtctggccaa tatggtcaag tccaatgccg ccgaagggcg
     7321 tctggttttc accaccagtc tcgccgaggc catggcagag gccgccatcg tcttcatcgc
     7381 agtcggaacg cccaccctgc gcggcgatgg ccatgccgac ctgagctatg tctatgcggc
     7441 ggcacgcgag attgccgggc tgatcagcgg gtttaccgtc attgtcacca aatcgaccgt
     7501 cccggtcgga accggcgagg aggtcgagcg gatcattcgc gaggcgcggc cggatgcgga
     7561 atttgccgtg gtgtcgaacc ccgaattcct gcgtgagggc gccgcgatcg gcgatttcaa
     7621 gcgccccgac cgcatcatca tcggcacgag cgacgagcgc gcccgcgcgg tgatggagga
     7681 ggtctaccgt ccgcttcatc tcaacgaggg gccgctggtg ttcatgggcc ggcgcgatgc
     7741 ggaactgacc aaatatgccg gcaatgcctt tctggcgatg aagatcacct tcatcaacga
     7801 gatggccgat ctggccgagg cggtcggcgc caatgtccag gccgtggcgc gtggcatcgg
     7861 cctcgacaag cgcatcggcg ccaagtttct caatcccggc cccggctatg gcggttcgtg
     7921 ctttcccaag gatacgctgg cgctggtcaa gacggcgcgc gatgccggcc gtccggtgcg
     7981 attgatcgag accgtggtgg acatcaacga tcagcgcaag gcggccatgg tcgacaagat
     8041 cgttgcagcc tcaggtggca gcgtcgccgg caagcgcatc gccatccttg gactggcctt
     8101 caagcccaac accgacgaca tgcgcgatgc tcccgcgctg gtcatcgtgc cgaaactcaa
     8161 gacgctgggc gccgatgtcg ttgccttcga tcccgaggcg catgaacagg ccgaaaggct
     8221 gatgcccggc ctcgcttttg ccaagggccc ttatgattgc atcgccggcg ccgacgccgt
     8281 ggtgatcgtg accgaatggg atgcgttccg cgcgctcgat ctggcgcggg tgcgcagtct
     8341 ggttcgcacc cctgtcatgg tggatctgag aaacatctac cgcgtcgaac aggtccgtgc
     8401 ccacggcttt gcctattcca gcatcggccg gcccgatcat ccgggcggct gatcgcggcg
     8461 gcgttcgggc ctacaatggg ttggcccgat accagtcgac caggcggcgc aacccgtcat
     8521 cgaggctgac cgagggcgta aagccggtga gagccgacag aagatcggta gcggcatagg
     8581 tttccggcac atcgcccggc tgcatcggca gaaagcgttt ctctgcggtc ttgcccagcg
     8641 cccgctccag cgagtcgatg aaatccatca gttcgaccgg tgccccgcgc ccgatattga
     8701 cgatgcggaa cggcgcatcg ggcgacagcg tgtccatcgg cccggccggc tggccaacca
     8761 ccggcggcgt cccggacagc ctgaggatcg cctcgacgat atcgtcgata taggtgaagt
     8821 cacgtcgcat ctggccatga ttgtagacat cgatgccgcg tccggcttcg atcgcctcga
     8881 tgaatttctg tggtgccatg tccggccgcc cccacgggcc atagaccgtg aagaagcgaa
     8941 acgccgtggt cggcaggttc caggcatggg aataggcatg cagcagcgcc tcgcccgcct
     9001 ttttggttgc ggcataaaag ctcaggggcc ggtcggcggc ctgggtttcg cggaacggcg
     9061 tggtgcgcgg cgctccatag acggaactgg tcgaggcaaa catcagatgc ttcgccccgg
     9121 ccgcgcgcgc cgcctccatc accgaaaaca atccggtgat gttgcaatca agataggtgg
     9181 ccggattttc caggctgtaa cgcacgcccg cctgtgcggc gagatggatc acgatatcgg
     9241 gctttgccgc ctccgcgatc gccagcgttg ccgtgcggtc ctcgagtcgc agccggtggc
     9301 cgctgtagcg ccggtgattg gccagcagcg ccgcgcgccg ttccttcagc cgcacgtcgt
     9361 aatagtcggt aaatccatcg atgccggtga ccgaatggcc gccctgcaac aggcgctcgg
     9421 ccacatggaa gccgatgaag ccggccgagc cggtgacgag aacgtgcatg acgggtgcct
     9481 cagaacaggc tccccttata cgcgatgcgt ccttaattca tggttggagt cgcgaaaaat
     9541 ggcatgaaag ccgaagcccg cccggtccgc cgcgcggtgc ggcgaaaacc ccgaaggcgc
     9601 cgcaggacgg cgcttccgga ttgcccggca ggtcacatcg gttcggggct cataccagcg
     9661 gccctgacaa ttgaccgttc ggggcaattg gcaggcggct ggtcaggagc gaatgcgacg
     9721 ccgagcaggg ccgaaggccc ggttgcgagg gacggcccg
//
LOCUS       DAOMXG010000090         9524 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_3793_length_9524_cov_9.082268, whole genome shotgun sequence.
ACCESSION   DAOMXG010000090 DAOMXG010000000
VERSION     DAOMXG010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 9524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9524
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3793_length_9524_cov_9.082268"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..2306
                     /locus_tag="PLQ11_07425"
     CDS             93..2306
                     /locus_tag="PLQ11_07425"
                     /inference="COORDINATES: protein motif:HMM:NF039586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HRK24767.1"
                     /translation="MSDSGPMPLKAESLRTQRRVRRIGEPLLFLLSVVAVLGTLALLM
                     GIGVAPSERRVKLALTLDTALALLLAVLVGFRLYRLYRARWSGRAGARLHLKVVSLLA
                     LASALPTAVVALAAMLTLDRGLTPWFSGNLKVLVENAGSIAGQFQQQLCQNVVREMRL
                     MAVDIERAYTGGVYSADRKLFREFMTSRAVYLGLPFAAIMRNDLSTIEKADVGVSSGQ
                     FTPSAEDFQLAGTDEPPCILSREVLGSVTKLKAIEGGYLVVGRPIDRRAIEFPLVAQA
                     GVEQYQELEARREATRFGIAVVFVLLTLLVTLAAVLLGLGLSDTIVQPIGRLIQATDE
                     VSSGNLYVQVPVRGDSDDVSHLGSTFNKMTNVLRTQNNSLVAANELIDRRRRFMEAVL
                     SGVPAGVLGVDPDGFIRISNPTADDLLGTPVGGLVNRPLAEVLPQLAARFHETQAGAS
                     RLMQETIEISRGGRNRTLSVRMTHEAGGGKGVVVTLDDITDLVTAQRTSAWADVARRI
                     AHEIKNPLTPIQLSAERIRRKFGKLIHEDRAVFDQCTETIVRQVEDIKRMVDEFSSFA
                     RMPKPTLAEEDLVATVKEVTFLMRLAHPDIDINDQVPDQPIALRFDRRLIAQAVQNII
                     KNATEAIAAVPSEQRGQGRIVLTIHSLPGGDVAVDVIDNGKGFPVENRERLLEPYMTE
                     RVGGTGLGLAIVAKILEDHGGRIELLDAPDGRGACVRLRLPAGSGDSAQERTPVA"
     gene            2303..3667
                     /locus_tag="PLQ11_07430"
     CDS             2303..3667
                     /locus_tag="PLQ11_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRK24768.1"
                     /translation="MSQEILIVDDEADIRELVSGILEDEGYRTRSARSADEALQAIET
                     RRPHLLFLDIWMQGSRLDGLQLLDVIKKGHPDLPVVMISGHGNIETAVSAIKRGAYDF
                     IEKPFKADRLVLVAARALEAQAMRREIQSLKSRSSQTERLVGRSSVINQLRQQIEKIA
                     PTNARILIAGASGAGKETAARTIHAASLRAAGPFVVINAATITPERMEEALFGVEAGD
                     GAARRSGALEDAHGGTLFIDEIADMPRETQGKILRVLVDQNFQRVGGATRVHVDVRVI
                     SSTSRVLEEEIAAGRFREDLYHRLSVVPLRIPALSERREDIPDLIETFMEQISGATGL
                     PKRLIAPDAMAVLQTHDWPGNVRQLRNNIERLMILAAGDPSIPVTAEMLPQDVGSAAP
                     LTPSGSGGERLLGLSLREAREIFEREYLIAQINRFGGNISRTAEFVGMERSALHRKLR
                     SLGV"
     gene            3943..4188
                     /gene="hfq"
                     /locus_tag="PLQ11_07435"
     CDS             3943..4188
                     /gene="hfq"
                     /locus_tag="PLQ11_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008546823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HRK24769.1"
                     /translation="MAHDRGQNLQDVFLNHVRKNKVPLTIFLVNGVKLQGVVTWFDNF
                     CVLLRRDGVSQLVYKHAISTIMPGAPISLSGPAEEEI"
     gene            4238..5602
                     /gene="hflX"
                     /locus_tag="PLQ11_07440"
     CDS             4238..5602
                     /gene="hflX"
                     /locus_tag="PLQ11_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HRK24770.1"
                     /translation="MPVFDAASVAEQAIAAATRTVVVGPYLSGERRPEASRRSFEARL
                     DEAVGLARAIDLDIVHQEIAPLKLPRPATYIGSGKIDQIKAIIETTEARLMMVDGALS
                     PVQQRNLEKALGVKVIDRTGLILEIFGRRARTREGALQVELAHLNYQKSRLVRSWTHL
                     ERQRGGFGFLGGPGETQIEADRRLIQERMRRIERDLEGVRRTRGLHRASRDKVPFPTV
                     ALVGYTNAGKSTLFNRLTRAEVLAKDMLFATLDPTTRVVDLPHGKRIILSDTVGFISD
                     LPTLLVAAFRATLEDVVEADVILHVRDIAHEDAEAQGADVTAILGDLGIGEAERRRMI
                     EVWNKADLLSAEARERLIGLSALGEAGKRPVLVSAVTGEGIAALLAEIEARLTHDHAR
                     FRLHLPAGDGAGLAWTYEVAEVLDRRQAEDGGVHLTIRVPPERLGRVERRFGAALKPL
                     DASD"
     gene            5701..7263
                     /gene="metG"
                     /locus_tag="PLQ11_07445"
     CDS             5701..7263
                     /gene="metG"
                     /locus_tag="PLQ11_07445"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HRK24771.1"
                     /translation="MTDAKTFYITTAISYPNGVPHLGHAYELMAADAIARFQRLDGRD
                     VFFLTGTDEHGLKMLQTARREGIAVRDLAARNAQAFRDMGPVFNATNDDFIRTSEPRH
                     YESCIALWKKMEERGDIYLSKYAGWYSVRDEAYFDESETSLAADGKRLAPSGAPVEWV
                     EEESYFFRLSAYQDRLLAHYEAHPDFIGPDTRRNEVISFVRRGLTDLSVSRNTFDWGV
                     PVPGSKDHVMYVWVDALTNYITAAGYPHETAPRWRYWPADLHLIGKDIVRFHTVFWPA
                     FLMSAGVPVPRRVFCHGFVFNRGEKMSKSLGNVVDPVAMAGLYGPDAMRYFLLREVPF
                     GQDGNYSHEAIVNRINADLANDFGNLAQRSLSMINKNCEGRVPQPGALTEADTNLLTA
                     IEGALILARQAMEDGFAMHTILNVLWGLIGDANRYFAAEEPWKLKKSDPARMNTVLYV
                     TAEALRRFGLLAQPFTPVGAARLLDLLAIPADRRSFARLGDADRLVPGMALPAPSRIF
                     PRFVEPEAGQAG"
     gene            7355..8146
                     /locus_tag="PLQ11_07450"
     CDS             7355..8146
                     /locus_tag="PLQ11_07450"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRK24772.1"
                     /translation="MLIDTHCHLDFPDFATDGVAALIDRARAAGVGRMVTISTRVKKV
                     ATYRALAEAHEAVFFSVGTHPHNAAEEPEVTAADLIRLSDHPKCVAIGEAGLDYHYDK
                     SPRPAQAASFRVHIAAARTTGLPLVIHARAADDDMIAILEEEYAKGAFPAILHCFSSG
                     AELARRGLALGFYLSFSGILTFRNSADLRAIAAAAPLDRLLVETDAPYLAPVPHRGKR
                     NEPGYVAHTAATLADVKSLPAAAMAGITTANALRVFHRMPPLEAA"
     gene            8143..8937
                     /locus_tag="PLQ11_07455"
     CDS             8143..8937
                     /locus_tag="PLQ11_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRK24773.1"
                     /translation="MSVRVTILGCGSSGGVPRVGQGWGACDPAEPRNRRRRCSILIES
                     GDPAAPTTVLVDTSPDLREQLIDADVRWLDGVVMTHDHADHSHGVDDLRPLTMVMRRR
                     IDVFADPVTTQSMQSRFGYCFEQAPGSSYPPILKLWPVAFGDPLTIEGRGGALSFATF
                     PVIHGDVTVLGLKFNNVVYTPDLNAIPDQSIAGLSGLDLWIIDALRPTPHPTHFSLSD
                     ALGWIERLKPKRAILTNLHSDLCYRALSARLPTHITAAYDGMVITA"
     gene            9079..9357
                     /locus_tag="PLQ11_07460"
     CDS             9079..9357
                     /locus_tag="PLQ11_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24774.1"
                     /translation="MTTMIACPGVRPLVRSDVPDPGLFGANENRWIDGAGLRRRRYAN
                     VAVIALAFGLLYGGVWIVTEFVRLQKIAACYEAGRQNCLPLDLNRRDR"
BASE COUNT         1648 a         2976 c         3110 g         1790 t
ORIGIN      
        1 gagcgatgct cgcggggcct ttttcgtcgt ataattgtcg catttcgaca acagtgttgc
       61 aaagttaagt cagtttcggt tctctggact ccatgtcaga ctcgggcccc atgccgttga
      121 aggcggagtc gctgcgcaca cagcgccgcg tgcggcggat tggcgagccg cttctgttcc
      181 tgctctccgt ggtcgccgtg ctgggcacgc tggcgctgct gatgggcatc ggcgtcgcgc
      241 cttccgaacg acgggtgaag ctggcgctga ccctggatac ggcgctggcg ctgttgcttg
      301 ccgtgctggt tggtttccgg ctctatcggc tctaccgggc ccgctggtcg ggacgggcag
      361 gggcgcgatt gcacctgaaa gtggtcagcc tgctggccct ggcctccgcc ctgccgacgg
      421 cggttgtggc gctggcggcg atgctgacgc tcgacagggg gcttacaccc tggttttcgg
      481 gcaacctcaa ggtgctggtc gagaacgccg gcagtatcgc cgggcagttc cagcagcaat
      541 tgtgccagaa cgtcgtgcgc gagatgcggc tgatggcggt cgatatcgag cgcgcctata
      601 ccggcggggt ctattcggcc gaccgcaagc tgtttcgcga attcatgacc tcgcgcgcgg
      661 tgtatctggg cctgccgttc gccgcgatca tgcgcaatga cctgtccacc atcgagaaag
      721 ccgatgtagg ggtctcgtcg ggccagttca cgccgagcgc cgaggacttc cagctggccg
      781 gaaccgacga gccgccctgc atcctgtcgc gcgaggtgct ggggtcggtt accaaactca
      841 aggcgatcga gggcggctat ctggtggtgg ggcggccaat cgaccggcgg gcgatcgaat
      901 ttccgctggt cgcccaggct ggtgtcgaac agtatcagga actggaggcg cgccgcgagg
      961 cgacgcggtt cggcattgcg gtggtgttcg tcctgttgac gctgctggtg acattggctg
     1021 cggtgctgct cggcctaggc ctgtcggata cgatcgtcca gccgatcggg cggctgatcc
     1081 aggcgacgga cgaggtttcc agcggcaatc tctatgtgca ggttcccgtg cgcggcgaca
     1141 gcgacgatgt cagccatctc ggttctacat tcaacaaaat gacgaacgta ttgagaacac
     1201 agaacaattc acttgttgca gcgaatgagc tgatcgaccg gcgccggcgc ttcatggagg
     1261 cggttctgtc gggcgtgccg gccggggtgc tcggtgtcga tccggacggc ttcatccgca
     1321 tctccaaccc gacggcggat gatcttctcg gcacgccggt gggcgggctg gtgaaccggc
     1381 cgctggccga ggtgctgccg caactcgccg cgcggttcca cgagactcag gccggcgcaa
     1441 gccggctgat gcaggaaacg atcgagatca gccggggcgg gcgcaaccgc actctgtcgg
     1501 tgcgcatgac ccacgaggcc ggcgggggca agggcgttgt cgtcacgctt gacgatatca
     1561 ccgatctggt gacggcgcag cgcacctctg cctgggccga tgtggcccgc cgcatcgccc
     1621 atgagatcaa gaacccgctg accccgatcc agctctccgc agagcgcatc cgccgcaagt
     1681 tcggcaaact gatccacgag gatcgcgcgg tctttgacca atgcaccgag acgatcgtgc
     1741 ggcaggtcga ggacatcaag cggatggtgg atgaattctc gtcttttgcc cgcatgccca
     1801 agccgacgct ggccgaggag gatctcgtcg ccacggtgaa ggaagtgacc ttcctgatgc
     1861 gtctcgccca tcccgacatc gacatcaacg accaggttcc ggaccagccg atcgcgctgc
     1921 gcttcgaccg gcggctgatc gcccaggcgg tgcagaacat catcaagaac gccaccgagg
     1981 cgattgcggc cgtgccatcc gaacagcgcg ggcagggccg gatcgtgctg accatccaca
     2041 gcctgcccgg cggggatgtg gccgtcgacg tgatcgacaa cggcaagggg ttcccggtgg
     2101 agaaccgtga acggctgctc gaaccctaca tgaccgaacg ggtcggcggc accggtcttg
     2161 gtctggccat cgtcgccaag atcctggaag accacggcgg cagaattgaa ttgctggacg
     2221 ctcccgacgg acgcggcgcc tgcgtgcgcc tgcggcttcc cgcgggcagt ggcgacagcg
     2281 ctcaggaaag gacaccagtt gcatgagcca ggaaatcctg atcgtcgacg acgaagccga
     2341 tatccgtgaa ctggtgtccg gaatcctgga agatgaaggc taccgcaccc gctcggcgcg
     2401 cagcgcagac gaagcgttgc aggcgatcga aacgcggcgc ccgcaccttt tgtttctcga
     2461 catctggatg cagggcagcc ggctcgacgg gctgcaattg ctcgatgtga tcaagaaggg
     2521 ccatcccgat ctgccggtgg tgatgatctc gggacatggc aacatcgaaa ctgcggtgtc
     2581 cgccatcaag cggggcgcct acgatttcat cgaaaagccg ttcaaggccg atcggctggt
     2641 gctggttgcg gcgcgggcgc tggaggcgca ggccatgcgc cgcgagatcc agtcgctgaa
     2701 gagccgctcg tcgcagaccg aacggctcgt cggccggtcc tcggtcatca accagttgcg
     2761 ccagcagatc gagaagatcg ccccgaccaa tgcccggatc ctcatcgccg gagcctccgg
     2821 agccggcaag gaaacagcgg cgcggaccat tcacgccgcc tcgctcaggg cggccggacc
     2881 gtttgtggtg atcaacgccg cgaccatcac cccggagcgg atggaagaag ccctgttcgg
     2941 cgtcgaggcc ggtgacggcg cagcaaggcg cagcggcgcg ctggaagatg cccacggcgg
     3001 cacattgttc atcgacgaga tcgccgacat gcccagggaa acccagggca agattctccg
     3061 cgtgctggtc gatcagaatt tccagcgcgt cggcggagcc acccgggtgc atgtcgatgt
     3121 gcgggtcatt tcctcgactt cgcgcgttct ggaagaggaa atcgccgccg gccggttccg
     3181 cgaggatctc tatcaccgcc tctcggtggt gccgctgcgc atcccggccc tgtccgagcg
     3241 ccgtgaggac atccccgacc tgatcgaaac cttcatggag cagatctcag gcgcgaccgg
     3301 tttgccaaaa cgcctgattg cgccggatgc catggcggtt ctgcaaacgc atgactggcc
     3361 cggcaatgtg cggcaattgc gcaacaacat cgagcgcctg atgattcttg ccgccggcga
     3421 cccgtcaatt ccggtgacag cggagatgct gccgcaggat gtcggttccg cagcgccgct
     3481 gactcccagc ggttcaggcg gggaacgcct gcttggcctg tcgctgcgcg aggcacggga
     3541 gatcttcgaa cgcgagtatt taatcgcaca gatcaatcga tttggaggaa atatttccag
     3601 aacggctgaa tttgttggaa tggaacgttc ggcattgcac aggaaactta ggtctctagg
     3661 cgtttaatgc tgtttgccta tggtcgggcc actaccgtta cggtcacggc tttgattcaa
     3721 atcaaaatgc ctcggttcgg ctgttgatga atgccagata aacacaatct tttggtccag
     3781 ttgtgtttcg cagcgctagt gaccggttgt tgacccgggg gcggcttttg tgccacttcg
     3841 agttgttggg tggccgggcc cgcggaatca tgcgcaagga ctcatgcgca gggccctgcg
     3901 gttcccccgg aagcagatca gagaattttg aggcttattg caatggcaca tgatcgcggt
     3961 cagaatttgc aggacgtgtt tctcaatcac gttcgcaaga acaaggtccc gctgaccatc
     4021 tttctggtga acggtgtgaa attgcagggc gtcgtcacct ggttcgacaa tttctgtgtc
     4081 ctgctgcgac gcgacggcgt ttcgcagctc gtctacaagc atgcgatctc taccatcatg
     4141 ccgggagcgc cgatttcctt gtccggtccg gccgaggagg agatctgatg cggacgcttt
     4201 accgggattg cgcttgagcg agcgcccgga cgatcatctg ccggttttcg atgcggcctc
     4261 cgtcgccgaa caggcgatcg cggcggcaac gcgcacggtg gtcgtcggtc cctatctttc
     4321 cggcgagcgg cggcccgagg catcgcggcg ctcgttcgag gcgcggctgg acgaagctgt
     4381 ggggctggcc cgtgccattg atctcgacat cgtgcatcag gagattgcgc cgctgaagct
     4441 gccgcgtccg gcaacctaca tcggctcggg caagatcgac cagatcaagg ccatcatcga
     4501 gaccaccgag gcgcggctga tgatggtcga tggagcgctg tcgccggtgc agcagcgcaa
     4561 tctcgaaaag gcgctcgggg tcaaggtcat cgaccggacc ggattgatcc ttgagatttt
     4621 cggacggcgc gcacgcaccc gcgagggcgc gctgcaggtg gaactcgccc atctcaacta
     4681 ccagaagagc cggctggtcc gctcctggac ccatcttgaa cgccagcgcg gcggcttcgg
     4741 ctttcttggc gggccgggcg aaacccagat cgaggcggac cgccggctga tccaggagcg
     4801 catgcgccgc atcgagcgcg acctggaggg cgtcaggcgc acgcgcggcc tgcaccgggc
     4861 cagccgcgac aaggttccgt tcccgaccgt ggcgctggtc ggctacacca atgccggcaa
     4921 atcgacgctg ttcaaccggc tgacccgcgc cgaggtgctg gccaaggaca tgctgttcgc
     4981 cacgctcgac ccgacaaccc gcgttgtcga tcttccgcat ggcaagcgga tcatcctgtc
     5041 cgatacggtg gggttcattt ccgatctgcc gacgctgctg gtcgccgcct tccgcgcgac
     5101 acttgaggat gtcgtggagg cggatgtgat cctgcatgtg cgcgacatcg cccatgagga
     5161 tgccgaagcg cagggcgccg acgtgacggc gattctcggc gatctcggca tcggagaggc
     5221 agagcggcgg cggatgatcg aggtctggaa caaggccgac ctgctgtcag ccgaggcgcg
     5281 cgagcggctg atcggactgt cggcgctggg cgaggccggg aagcgtccgg tcctcgtgtc
     5341 agccgtgacg ggggagggga tcgccgcgtt gctcgccgag atcgaggcac ggctgaccca
     5401 cgaccacgcg cgattccggc ttcatctgcc ggccggtgac ggcgccgggc tcgcctggac
     5461 ctacgaggtt gccgaggttc tggaccgtcg gcaggcggag gacggaggtg tccacctgac
     5521 catccgcgtg ccgcccgagc ggctggggcg ggtggagcga cggttcggcg cggcgctcaa
     5581 gccgctggac gcctcggact gagtccgctc ctcgactccg ggccggcatt cgccgcgcaa
     5641 aaatgtccca gccttgtcga atgcattctc aaaccgatgc ggagcggcta aggatgcgga
     5701 atgaccgatg caaaaacctt ctacatcacc acagcgattt cctaccccaa tggcgtgccg
     5761 catctcggcc atgcctacga actgatggcg gctgacgcga tcgcgcggtt ccagcggctt
     5821 gacgggcggg acgtgttctt cctaaccggc accgacgagc acggcctcaa gatgctgcag
     5881 acggcccgcc gcgaggggat cgccgtgcgc gaccttgccg cccgcaacgc tcaggcgttc
     5941 cgcgacatgg gcccggtgtt caacgccacc aacgacgatt tcatccgcac ctccgagccc
     6001 cgccattacg agtcctgcat cgcgctgtgg aagaagatgg aagaacgcgg cgacatctat
     6061 ctgtcgaaat atgccggctg gtattcggtg cgggacgagg cctatttcga cgagagcgag
     6121 accagccttg ccgccgatgg caagcggctg gcgccctccg gcgctccggt cgaatgggtg
     6181 gaggaggaaa gctacttctt ccgcctgtcc gcctaccagg atcgcctgct ggcccattac
     6241 gaggcgcatc cggatttcat cggtccggac acaaggcgca acgaggtgat cagcttcgtc
     6301 aggcgcggcc tgaccgacct gtcggtcagc cgcaacacct tcgactgggg cgtgccggtg
     6361 ccgggcagca aggaccacgt gatgtatgtc tgggtcgatg ccctgaccaa ttacatcacc
     6421 gctgccggct atccgcacga gacggcgccg cgctggcgct actggccggc ggatctgcat
     6481 ctgatcggca aggatatcgt ccgtttccat acggtgttct ggccggcctt cctgatgagc
     6541 gccggcgttc cggtgccgag gcgggtgttc tgccacggct tcgtgttcaa ccgcggcgag
     6601 aagatgtcga agtcgctcgg caacgtggtc gatcccgtgg ccatggccgg gctctacggc
     6661 cccgatgcga tgcgctattt cctgctgcgc gaagtgccgt tcggccagga cggcaactac
     6721 agccacgagg cgatcgtcaa ccgcatcaat gccgatctcg ccaatgattt cggcaacctc
     6781 gcccagcgct cgctgtcgat gatcaacaag aactgcgagg gcagggtgcc tcaaccgggc
     6841 gctctcaccg aggccgatac caatctgctg accgccatcg agggcgcgtt gatcctcgcc
     6901 cggcaggcga tggaggacgg ctttgccatg catacaatcc tcaacgtgct gtgggggctg
     6961 atcggcgatg ccaaccgcta tttcgccgca gaagagccgt ggaagctgaa gaaatccgac
     7021 cctgcacgca tgaacacggt gctctatgtc accgccgagg cgttgcgccg gttcggcctg
     7081 ctggcccagc cgttcacgcc ggtgggcgcg gcaagactgc tcgatcttct ggcaattccg
     7141 gccgatcgcc gcagcttcgc ccggctgggc gatgctgacc ggttggtgcc cggcatggcg
     7201 cttccggcgc cgtcccgcat cttcccgcgc tttgtggaac cggaggccgg gcaggcgggg
     7261 tgatcgcccc tgcaagcctg ttggtcatcc agcggacttg acgcgggcgc acgaaccggc
     7321 cttacacaat ggccatgccg cgcccggacc tgccatgctg atcgacaccc attgccatct
     7381 cgactttccc gatttcgcaa cggacggcgt cgcggcgctg atcgaccgcg cgcgggccgc
     7441 cggtgtcggg cgcatggtga cgatctcgac gcgggtgaag aaggttgcaa cctaccgcgc
     7501 cctggcagaa gcgcatgagg cggtgttctt ctcggtcggc acgcatccgc ataacgccgc
     7561 cgaggaaccg gaggtgacag ccgccgatct gatccggctg tccgaccatc ccaaatgtgt
     7621 ggcgattggc gaagcggggc tcgactacca ttacgacaag tcgccacgcc cggcgcaggc
     7681 tgcctcgttc cgcgtccata tcgcggcggc gcgcaccacc ggcctgccgc tggtgatcca
     7741 cgcccgcgcg gccgatgacg acatgatcgc cattctcgag gaggaatatg cgaaaggcgc
     7801 cttcccggcg atcctgcatt gcttctcgtc cggggcggaa ctggcgcggc gcgggctggc
     7861 gctggggttc tatctgtcgt tttccggcat cctgacgttc aggaattccg ccgatctgcg
     7921 cgccatcgcc gctgcggcgc cgctggaccg gctgctggtc gagaccgacg cgccgtatct
     7981 ggcgccggtg ccgcatcgcg gcaagcgcaa cgagccgggc tatgtcgccc atacggcggc
     8041 cacactggcg gatgtgaaga gcctgccggc agcggcaatg gccggcatca ccacggccaa
     8101 tgcgctcagg gtgttccacc gcatgccacc gctggaggct gcatgagcgt ccgggtgaca
     8161 atcctcggct gcgggtcatc tggcggtgtg ccgcgcgtcg ggcaggggtg gggcgcctgt
     8221 gatccggccg aaccgcgaaa ccgccgccgc cgctgttcca ttctcattga atcgggcgat
     8281 cctgcggctc ctacgacggt tctggtcgat acctcgcccg acctgcgcga acagttgatc
     8341 gatgccgatg tgcgctggct ggacggcgtg gtgatgacgc atgatcatgc tgatcattcc
     8401 catggcgttg acgacctccg gccgctgacc atggtgatgc gccggcgcat cgacgtcttt
     8461 gccgatcctg tcaccacaca gtcgatgcaa tcgcgcttcg gctattgctt cgaacaggcg
     8521 ccgggcagca gctatccgcc gattctgaag ctctggccgg ttgcgttcgg cgatccgctg
     8581 accatcgaag ggcggggcgg agcgctgagc tttgccacct ttccggttat ccacggcgat
     8641 gtgaccgtgc tcggccttaa gttcaataat gtcgtctata cccctgatct caatgcgatt
     8701 ccggatcaat ccatagctgg tctgtcaggt ctcgatctgt ggatcatcga tgcgctgcgg
     8761 ccgacgccgc atccgaccca tttctcgctt tctgatgcgt taggctggat cgaacgattg
     8821 aagccaaagc gcgccattct gaccaatctg cactcggacc tgtgctatcg cgccctgagc
     8881 gcgcgactgc cgacgcatat cactgctgcc tacgacggga tggtcatcac ggcgtgacga
     8941 tgccggacgg ccacgttttg ctgctggcaa tgtgtgcggt ttgcagctaa cattaaccgt
     9001 atcggctccg gcgttcgtgt catgtcagtc aatcacccgg ctcctgtttc agaccagacg
     9061 ccccgcgagg ccgcgcgcgt gacgacgatg attgcctgtc cgggcgtgcg tccgctggtc
     9121 cggtcggacg tcccggatcc ggggctgttc ggcgcaaatg aaaaccgctg gatcgatggc
     9181 gccggattgc ggcggcggcg ttatgcgaat gtcgcggtga tcgcgctggc gttcgggctt
     9241 ttgtatggcg gcgtgtggat cgtgaccgaa ttcgtgcggc tccaaaagat cgccgcatgc
     9301 tatgaggcgg gccgccagaa ttgcctgccg ctggatttga acaggcgcga ccgctgatca
     9361 tccggcatgt cagtattttc tggcaacatg ctggaaagac aagacaatag ctgcacttga
     9421 gaagcttaaa tcgcatcact ctgccatgcc ttgtggttcc ataatatgtc ttatgcgaat
     9481 cacgaatgta agtccccgat aaagatgtca ccggtctgcc cgtt
//
LOCUS       DAOMXG010000091         9501 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_3805_length_9501_cov_7.902181, whole genome shotgun sequence.
ACCESSION   DAOMXG010000091 DAOMXG010000000
VERSION     DAOMXG010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 9501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9501
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3805_length_9501_cov_7.902181"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="PLQ11_07465"
     CDS             complement(<1..824)
                     /locus_tag="PLQ11_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRK24775.1"
                     /translation="MVAWHDFFTTSQDGLRLYARRLGPAEGRLTPVICLPGLARTTLD
                     FEEVAQHLAASGDRTVWAFDYRGRGRSDRDPDWTHYDLRVELGDVGDQLTALGIPPAV
                     FLGTSRGGMLTTLMAIARPAALRGAILNDIGPVIEGKGLARIKSYVGKLPRPETLEQG
                     SEILKRISSSQFTAADPATWLKMAGRTWLETPDGLVPRYDVALMKTLEQLDLDAPLPD
                     LWPQFGALSHVPLMVIRGSNSDLLSAETTAEMARRHPDCRVLEVPGEGHAPLLADAP"
     gene            complement(884..3325)
                     /locus_tag="PLQ11_07470"
     CDS             complement(884..3325)
                     /locus_tag="PLQ11_07470"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRK24776.1"
                     /translation="MASSASRIRPRRTLRLLLAGLSTVAWLAAAGAQDHAADLDVREA
                     LAAMDRAQAEAGEALAAASRALAEADTAAAMLPPGLSLPDLASLTPPEWLTGSADDDA
                     LMTGSLAVPPVFEPEPLTLPSARPPLLGSVKRLEPVMDLAAGEMFNPDYVRRPSVTAV
                     PPADLALMQQALTEYRRNAIAAGDLLAERIEDEAARAALEWSVIRLKRQGLGSARIAR
                     FVSTHGDFPMRAWLIGRIEDALFVEQPSPESVFWFYGDRLPSTATGMVVLARALKQRG
                     EGFAARELARRAYRDGKTNAALRSAITREFSGLITAADQRYLAERLIYDGESAEGLRV
                     AAEAGPLVQQIAQVLTASVNETGALAGLLNKLDPALQREPPVIFARAQHLRRSNAYGD
                     AARALLSVPADSEAIIDGDEWWIERRLLSRKLLDAGEPLLAYRIVATHQAESAGHRVD
                     AEVQAGWIALRFLGEAEQARQHFETARQAAETPHAQARAQYWLGRAGVALGDPAAMLA
                     YGAAAQHRHAFHGQLALAELGATEIGETEVRLDVGALELATRTLAVRTIRALLDAEAR
                     DLAQPLIVDAAKTAPTLEANVALGDLVARYNLPRLTVIAGKMAMQSGRKAEEHAFPTF
                     GIPPFEATQDSAEAAIVYSIARQESEFEAGAVSHAGARGLMQLMPATARATASRNRLP
                     FDVGALTRDASLNARIGAAHLGELFREYNGSYLLAFAAYNAGGGRVKEWIAAYGDPRD
                     PAIDPIDWIERIPITETRHYVQKILENVQIYRMRLSGNPALGIAADLKRGLRAPRPQA
                     LSGLAETAPAGAARP"
     gene            3513..4400
                     /gene="dapA"
                     /locus_tag="PLQ11_07475"
     CDS             3513..4400
                     /gene="dapA"
                     /locus_tag="PLQ11_07475"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HRK24777.1"
                     /translation="MTAKTFHGSNTALVTLFRDGKLDEKANRAHVNWQIEQGTHGLVP
                     VGTTGESPTLSHDEHKKVVEWCIQEAKGRVPVIAGAGSNSTEEAIDFSRFAEKAGADG
                     LLVVTPYYNKPTQEGLYQHYKAINDAVGIPILIYNIPGRSVIDMSVETMARLHELKNI
                     AGVKDATAKLDRVSLQRQAMGPEFIQLSGEDGTALGFNAHGGHGCISVTSNVAPRLCA
                     DFQNACARGDYAAALKIQDRLMPLHTAIFLETNPSPIKYALSLIRPVTADVRLPMVPC
                     SDGVKRAVEAAMRHAGILN"
     gene            4531..5004
                     /gene="smpB"
                     /locus_tag="PLQ11_07480"
     CDS             4531..5004
                     /gene="smpB"
                     /locus_tag="PLQ11_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HRK24778.1"
                     /translation="MSKAPSTRYKIAADNRKARYNYEIGQVFEAGIALTGTEVKSLRG
                     GKATIGESYASVERDEVFLINAYIPEYLQANRFNHETKRPRKLLLHRREIDRLISAVA
                     REGMTIVPLKLFFNEKGRAKVEVALARGKKLHDKRETEKKRDWDREKGRLLRDRG"
     gene            complement(5005..5643)
                     /locus_tag="PLQ11_07485"
     CDS             complement(5005..5643)
                     /locus_tag="PLQ11_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRK24779.1"
                     /translation="MPSNEPGKACGLCPRLVAFRATARAAHPDWHNAPVPNFGPVAAP
                     MLIVGLAPGLQGANRTGRPFTGDWAGDLLYETLLEFGFATGCYEERADDTLQLTQGRI
                     VNAVRCVPPENKPTGAEVATCRQFLAASMAEMTGLKAIVALGRIAHDSVVRTLNLPAA
                     RFGFAHGAQHALPDGRVLFDSYHCSRYNTNTGRLTPAMFRAVFQAVRGHIGA"
     gene            complement(5650..6297)
                     /locus_tag="PLQ11_07490"
     CDS             complement(5650..6297)
                     /locus_tag="PLQ11_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HRK24780.1"
                     /translation="MISSDRIALFIDGANLYAAARALGFDIDYRKLLEEFRTRGYLVR
                     AFYYTAIIEDQEYSSLRPLVDWLDYNGYMVVTKPAKEFTDSTGRRKIKGNMDIELAVD
                     AMELAPHITHMVLFSGDGDFRSLVEAMQRKGVRVTVVSTVTTQPPMMADDLRRQADTF
                     LDLAELQAKIGRAPSERPVREPRDFTPRGAESPRAMPAFLNRPAGTTRSTPDEQT"
     gene            6616..7014
                     /gene="rpoZ"
                     /locus_tag="PLQ11_07495"
     CDS             6616..7014
                     /gene="rpoZ"
                     /locus_tag="PLQ11_07495"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HRK24781.1"
                     /translation="MARVTVEDCIDKIDNRFELVLLAAHRARVLASGSQPLVKRDNDK
                     NPVIALREIADERLSPGDLKEEFIHSMQKFVEVDEPEPEPVPRIAAAAQPVVGGGSDD
                     SDVSFDRMSEEELLRGLDGLVPPSDSDDDD"
     gene            7130..9319
                     /locus_tag="PLQ11_07500"
     CDS             7130..9319
                     /locus_tag="PLQ11_07500"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HRK24782.1"
                     /translation="MMRQYELVERVRRYNPNADEDLLNKAYVYAMQAHGHQKRASGDP
                     YFAHPLEVAAILTELKLDDATIVAAMLHDTIEDTAATREEIDRLFGTEIGALVDGLTK
                     IKKLDLVSKRAAQAENLRKLLLAISDDVRVLLVKLADRLHNMRTLHYVPPEKRARIAE
                     ETLDIYAPLAGRMGMQQMREELEDLSFRTLMPDARTMVVKRLEELRERSGELIAAIER
                     ALTAKFTAAHIDASVKGREKTPYSIWKKMERKAVAFEQLSDIFGFRIIVATTEDCYRA
                     LWVAHTSWPMVPGRFKDYISTPKQNDYSSLHTTVVGPRHQRVELQVRTEDMNEVAEQG
                     VAAHAVYKDGRPGADPSESNAFQWLRRTIELLAEGDSPEEFLEHTKLELFHDQVFCFT
                     PKGRLIALPRGATPIDFAYAVHTDVGNTTVGCRINGTVAPIVSELNNGDEVEIIRAEN
                     HVPPPAWESLVVTGKARAAIRRATRDAVRRQFAGLGQKIIERAFTRSNRAFSTEKLIA
                     ALPRLARQSVDDVLAAVGRGEMYSGDVVKAVYPDFKDEQVKGATPARGAGWFELSTAE
                     SLKFKVPGQGNQTHALPIRGLTGELPVRFAPNGGAVPGDRIVGILTPGEGITVYPIQS
                     PALSAFEDEPRRWLDVRWDLDEAAKSRYPAQLHLEVINEPGALASITRVIGDADANID
                     SISMMSRTADFREMLIDIEVWDLKHLNAIISELRTTAQVSKVERVNG"
BASE COUNT         1775 a         3057 c         3048 g         1621 t
ORIGIN      
        1 ggcgcatcgg ccagcagcgg ggcatggcct tcgccgggca cttcgagcac gcggcaatcg
       61 ggatggcgcc gcgccatctc tgccgttgtt tccgccgaaa gaaggtccga attgctgccc
      121 cggatcacca tcagcggcac atgtgacaat gcgccgaact gcggccagag gtccggaagc
      181 ggtgcatcga gatcaagctg ttcgagcgtt ttcatcaggg ccacatcgta acgcggcacc
      241 agcccgtccg gcgtctccaa ccaggtgcgg ccggccatct tcagccaggt ggctggatcg
      301 gctgccgtga actgcgaact tgaaattctc ttcaggattt cagaaccttg ctccagggtc
      361 tcggggcgcg gcagcttgcc gacatagctc ttgatgcgcg ccagcccctt gccctcgatc
      421 accggcccga tgtcgtttag gatggcgcct ctgagtgccg caggacgggc aatcgccatc
      481 agcgttgtca gcattccgcc ccgcgatgtg ccgaggaata cggcgggcgg aatgccgagc
      541 gccgtcaact ggtcgccgac atcgccgagt tcgacccgca ggtcataatg cgtccagtcc
      601 ggatcgcgat cagagcggcc gcgaccacga tagtcgaagg cccagaccgt gcggtcaccc
      661 gaggcggcaa gatgctgcgc cacctcctcg aaatcgaggg tcgtgcgcgc cagccccggc
      721 aggcagatga ccggcgtcag ccgcccctct gccgggccga gacggcgtgc atagagcctc
      781 agcccgtcct ggctggtcgt gaagaagtcg tgccaggcca ccatctccgc ctcctgctgc
      841 atgagcagca aggtttaggg cccgggactg gtccgatcaa gcctcagggg cgggcagcgc
      901 cggcgggggc ggtttcggcc agaccgctga gggcctgcgg acgcggcgcc ctgaggccgc
      961 gcttcaagtc ggcggcaatt ccgagcgccg ggttgccgct cagccgcatc cggtagatct
     1021 gcacgttttc gaggatcttc tgcacatagt gccgcgtctc ggtgatcgga atgcgctcga
     1081 tccagtcaat cggatcgatg gcgggatcgc gcggatcacc ataggcggcg atccattcct
     1141 tcacccggcc gccgccggca ttgtaggcgg cgaacgccag cagatagctg ccgttgtatt
     1201 cgcggaacaa ttcgccgaga tgggcggcgc cgatgcgtgc attgagcgag gcatcgcggg
     1261 tcaacgcgcc gacgtcgaac ggcaggcggt tgcggcttgc ggttgcccgt gccgtggccg
     1321 gcatcagctg catcaatccg cgcgcgcccg catgcgacac cgcgccggcc tcgaactcgc
     1381 tttcctgccg ggcgatcgaa tagacaatgg ccgcttctgc gctgtcctgg gtggcctcga
     1441 acggcggaat gccgaacgtg ggaaacgcat gttcttcggc cttgcggccg ctctgcatcg
     1501 ccatttttcc ggcgatcacc gtcaggcgtg gcaggttgta gcgtgccacc agatcgccga
     1561 gggcgacatt ggcctcaagt gtgggcgcgg ttttggccgc atcgacgatc agcggctggg
     1621 caagatcgcg cgcttccgca tcgagcaggg cgcgaatggt ccgaaccgcc agggtgcggg
     1681 tcgccagctc cagcgccccg acatccagcc gcacctcggt ctcgccgatt tccgtagccc
     1741 cgagttccgc aagtgcaagc tggccgtgga atgcatggcg atgctgtgcg gcggcgccat
     1801 aggcaagcat ggcagccgga tcgcccaacg caacgccggc gcggcccagc caatactggg
     1861 cgcgcgcttg cgcatgcggg gtctcggcag cctgccgtgc cgtctcgaaa tgctggcgcg
     1921 cctgctcggc ctcgccaaga aaccgcaggg cgatccagcc cgcctgcacc tcggcatcga
     1981 cacgatggcc ggcgctttcg gcctgatgcg tggcgacgat ccggtaggca agcaggggct
     2041 cgccggcgtc gagcagcttg cggctgagca gccgccgctc gatccaccat tcgtcaccgt
     2101 cgataatcgc ctcgctgtct gccggaacgc tgagcagggc ccgtgccgca tcgccatagg
     2161 cattgctgcg cctgagatgc tgtgccctgg caaagatcac cggcggctcg cgctgcaagg
     2221 ctggatcgag cttgttcagc agccccgcca aagcgccggt ctcgttgacc gaggcagtga
     2281 gcacctgggc gatctgctgc accagcggcc cggcctcggc cgcgacgcgc agcccttcgg
     2341 cgctctcgcc atcgtagatc agccgctcgg ccagatagcg ctggtccgca gccgtgatca
     2401 ggccggaaaa ttcgcgggta atggcgctgc gcagggcggc gttggtcttg ccatcgcgat
     2461 aggcccgccg cgccagctcg cgcgcggcaa atccctcgcc gcgctgtttg agggcccggg
     2521 ccagaaccac catgccggtg gcagtggacg gcaggcgatc gccgtagaac cagaacacgg
     2581 actcggggct gggctgctcg acgaacaagg catcctcgat gcggccgatc agccaggccc
     2641 gcatcggaaa gtcaccatga gtcgaaacaa agcgggcaat gcgcgccgat cccagcccct
     2701 gccgcttcag ccggatgacg ctccattcga gcgcagcgcg cgccgcctca tcctcgatgc
     2761 gctcggccag aagatcgccg gcggcgatcg cattgcgcct atattcggtc agtgcctgct
     2821 gcatcagcgc cagatcggcc ggcgggacgg cggtgacact gggccggcgc acataatcgg
     2881 ggttgaacat ttcgccggcg gcaagatcca tcaccggctc caggcgcttc accgatccaa
     2941 gcagcggtgg tctggcggag ggcagggtga gcggttcggg ctcgaacacc ggtgggaccg
     3001 ccagcgatcc ggtcatcagc gcatcatcat ccgcgctgcc cgtcagccat tccggcggag
     3061 tgaggctcgc aaggtcggga agcgacaggc cagggggcag catggccgcc gctgtatcgg
     3121 cctcagccaa ggcccggctt gcggccgcca gcgcttcgcc ggcctctgcc tgggcgcggt
     3181 ccatcgcggc gagcgcctcc cgcacgtcga ggtctgcggc atgatcctgc gcgccggcgg
     3241 cggccagcca ggcgaccgtc gacagcccgg cgagcagaag tcgaagcgtg cggcgcggtc
     3301 tgatgcgcga tgctgacgag gccatcggcc gtcctcttgc gacgggaagc atccggcagt
     3361 ttcgccagcg gcgcattacg atttcgttaa gcatgccgaa actgcggcac ctcaccacgt
     3421 ttctggcctt ttcagcgccg agttgggcgg ttatgaggaa gccaaccacc attgacaatc
     3481 gtccggccgt cccggccagg agtgaccgca ccatgaccgc aaagaccttt cacggctcca
     3541 ataccgcgct ggtgaccctg ttccgcgatg gcaagctgga cgagaaggcc aaccgggccc
     3601 atgtgaactg gcagatcgag cagggcaccc acgggctggt tccggtcggc accacgggcg
     3661 aaagcccgac gctgagccat gacgaacaca agaaggtcgt cgaatggtgc attcaggagg
     3721 cgaagggccg cgttccggtg attgccggcg ccggctccaa ctcgaccgag gaagcgatcg
     3781 atttttcccg ctttgccgag aaggccggcg cggacgggct gctggtcgtc acgccctatt
     3841 acaacaagcc gacgcaggaa gggctctatc agcactacaa ggcaatcaac gatgcggtcg
     3901 gcatcccgat cctgatctac aacattcccg gacggtcggt gattgacatg tcggtcgaga
     3961 ccatggcgcg gctccatgag ctcaagaaca tcgccggggt caaagatgcc accgcgaagc
     4021 tcgaccgcgt gtcgctgcaa cggcaggcga tggggccgga gttcatccag ctttcgggcg
     4081 aggatggcac cgcgctcggc ttcaacgccc atggcggtca cggctgcatt tcggtcacct
     4141 cgaatgtggc gccgcggctc tgcgccgatt tccagaatgc ctgcgcgcgg ggcgattatg
     4201 ccgctgcgct gaagatccag gaccggctga tgccgttgca caccgcgatc tttctggaaa
     4261 ccaacccctc gccgatcaaa tatgccctgt cgctgatccg cccggtcacg gcggatgtgc
     4321 ggctgccgat ggtgccgtgc tccgatggcg tcaagcgcgc ggtcgaggcg gcgatgcgcc
     4381 atgccggtat tctgaactga ggccctgccg ccgctcgtcc gcacaccgga cgacggccgc
     4441 tcgccgggca gaacggctgc gggggcacgc ccgacccgaa ttcgcaacgc gcgggttggc
     4501 acgggacgcc cggtgcccta tctgtcgtcc atgtccaaag ccccctccac ccgctacaag
     4561 attgccgccg acaaccgcaa ggcgcgctac aattacgaga tcggccaggt tttcgaggcc
     4621 ggcattgcct tgacgggaac cgaggtgaaa tcgctgcgcg gcggcaaggc gacgatcggc
     4681 gaaagctatg cctcggtcga gcgcgacgag gtgtttctca tcaacgccta cattccggaa
     4741 tatctgcagg ccaaccgctt caaccatgag accaagcggc cgcgcaaact gctgctgcat
     4801 cgtcgcgaga tcgatcggct gatcagcgcc gttgctcgcg aaggcatgac catcgtgccg
     4861 ctcaagctgt tcttcaacga aaagggccgc gccaaggtcg aggtcgcgct cgccaggggc
     4921 aagaagctgc acgacaagcg cgaaaccgag aagaagcgcg attgggatcg cgagaaaggt
     4981 cgtctgctgc gcgatcgtgg atgatcaagc gccaatatgc ccgcgcaccg cctgaaacac
     5041 cgcgcggaac atggcgggcg tcagccggcc tgtattggtg ttgtagcgcg agcaatgata
     5101 gctgtcgaac agcacccgcc catccggcaa agcgtgttgc gccccatggg cgaagccgaa
     5161 ccgtgccgcc ggcaggttga gcgtgcgcac cacgctgtca tgggcgatgc ggcccagcgc
     5221 aacgatggcc ttcagacccg tcatttccgc cattgaggcg gcaaggaatt gccggcaggt
     5281 ggcgacttcc gcgcccgtcg gcttgttctc ggggggcacg cagcgcacgg cattgacgat
     5341 gcggccttgc gtcaactgga gcgtatcgtc cgcccgctct tcatagcagc cggtggcaaa
     5401 gccgaattcc agcaatgtct cgtagagaag atcgcccgcc cagtcgccgg tgaacggccg
     5461 gccggtgcga ttggcgccct gcaaccccgg agcaagcccg acaatcagca tcggagcagc
     5521 aaccggaccg aaattcggta ccggcgcatt gtgccagtca ggatgcgccg cccgcgccgt
     5581 cgcgcggaac gcaacaagac gcggacacag cccgcaggcc ttccccggct cgttcgaagg
     5641 catggagttt caggtctgct cgtccggggt ggagcgcgtc gtgccggcgg gacgattgag
     5701 aaaagccggc atggcgcggg ggctttccgc tccgcgcggt gtgaaatcgc gcggctcgcg
     5761 cacaggccgt tccgaaggcg cgcggccgat cttggcctga agctcggcca gatcgaggaa
     5821 cgtatcggcc tggcgcctca gatcatcggc catcatcggc ggctgggtgg tgacagtgga
     5881 gaccacggtc acccgcacgc ccttgcgctg catcgcctcg accaggctgc ggaaatcgcc
     5941 atcgccggag aacaggacca tatgggtgat atgcggggcc agctccatcg catcgacggc
     6001 cagctcgata tccatgttac ccttgatctt ccggcgcccg gtcgaatcag tgaactcctt
     6061 cgcgggcttg gtgacaacca tatagccatt ataatcgagc cagtcgacca acggccgtaa
     6121 cgaagaatat tcctggtctt cgataatagc agtatagtaa aaagcgcgga caagataacc
     6181 gcgggtgcgg aattcttcga gcagtttgcg atagtctata tcgaaaccaa gggctcgtgc
     6241 ggcagcatag aggtttgcgc catcgataaa gagcgcgatg cgatcagagg aaatcattcg
     6301 tgttccttgt aaaatgacaa tttctgaagg gtcggccaat tcgtccttgc caacgctggg
     6361 aaagggttat cgccaaatcc cctcgaatga aagaaaaatc gcccaaaaat gcaacaaatc
     6421 ccaactggtg agatgatgtg acttgggttc atttttttct tgcacaacaa ttgtgcatcg
     6481 ctgcaatttt actctgcatg acgtgcatgc gagatggggc atgcccggca tcgcgaaatg
     6541 gttgccgtgg ccgatcaaat cggttagaag cggccagctt tccatgccca actgtcgttc
     6601 aaggagcgcg tcgatatggc ccgcgtgacc gtcgaagatt gcatcgacaa gatcgataac
     6661 cggttcgaac tggttctgct ggccgcccat cgcgcccgcg ttctggccag cggttcgcag
     6721 ccgctggtca agcgtgacaa cgacaagaat ccggtcatcg ccctgcgcga gatcgctgac
     6781 gagcggctgt cgcccggcga tctcaaggag gaattcattc actcgatgca gaagttcgtc
     6841 gaggtggacg agccggagcc ggagcccgtt ccgcgcatcg cggcggcggc ccagccggtc
     6901 gtcggcggcg gctccgacga ttccgatgtc tcgttcgatc gcatgtcgga agaggaactg
     6961 ctgcgcggcc tcgacggtct cgtgccgcct tcggacagcg acgacgacga ctgagccagg
     7021 cctgtcattg ctttgccaaa acgggcgcga ccgctggaca ggcgggccgc gcccgttgca
     7081 cattgagcgc ttgtgacgca cgcatggcgc gtagtggggt catactgcaa tgatgcggca
     7141 atacgaactc gttgaacggg ttcgccgata caatccgaat gctgacgagg acctgctcaa
     7201 caaggcctat gtctatgcca tgcaggcgca tggccaccag aagcgcgcgt cgggcgatcc
     7261 ctatttcgcc catccgcttg aagtcgctgc cattctgacc gaactcaaac tcgacgacgc
     7321 caccatcgtc gcggccatgc tgcacgacac gatcgaggac accgccgcaa cccgcgagga
     7381 gattgaccgg ctgttcggaa ccgagatcgg ggcgctggtc gatgggctga ccaagatcaa
     7441 gaagctcgat ctcgtctcca agcgagccgc tcaggcggag aacctgcgca agctgctgct
     7501 ggcgatctcg gacgatgtgc gcgtgcttct ggtcaagctg gccgaccggc tgcacaacat
     7561 gcgcacgctg cattatgtgc cgccggaaaa gcgcgcccgc atcgccgagg aaacgctgga
     7621 catctatgcc ccgcttgccg ggcgcatggg catgcagcag atgcgcgagg agcttgagga
     7681 cctgtcgttc cgcaccctga tgcccgacgc gcgcaccatg gttgtcaagc ggctggaaga
     7741 gctgcgcgag cgctcgggag agctgatcgc ggcgatcgag cgggcgctga cggccaagtt
     7801 caccgccgcc catatcgatg cctcggtcaa ggggcgggag aaaacgccct attccatctg
     7861 gaagaagatg gagcgcaagg cggtcgcctt cgagcaattg tcggacattt tcggtttccg
     7921 catcatcgtt gcgacaactg aggattgcta ccgggcgctg tgggttgccc ataccagctg
     7981 gccgatggtg cccggccggt tcaaggatta catctcgacc cccaagcaga acgactattc
     8041 ctcgttgcac acgacggtgg tgggtccgcg ccatcagcgt gtcgaattgc aggtccgcac
     8101 cgaggacatg aatgaggtcg ccgagcaggg cgttgccgcc catgccgtct acaaggatgg
     8161 ccgaccgggc gctgacccgt ccgaatccaa tgccttccag tggctgcgcc gcaccatcga
     8221 actgctggcc gagggggaca gtcccgagga atttcttgaa cacaccaagc tcgaactgtt
     8281 ccacgatcag gttttctgct tcacccccaa ggggcggctg atcgcgctgc cccgcggtgc
     8341 aactccgatt gattttgcct atgccgtgca caccgatgtc ggcaatacaa cggtgggctg
     8401 ccgcatcaac ggcacggtgg cgccgatcgt ttcggaactc aacaacggcg acgaagtgga
     8461 aatcatccgc gccgagaacc atgttccgcc ccccgcctgg gagagtctgg tcgtcaccgg
     8521 caaggcccgc gccgccatcc gccgcgccac gcgcgacgca gtgcgacgcc aattcgccgg
     8581 gctgggccag aagatcatcg aacgcgcctt cacccggtcg aaccgggcgt tttcgacaga
     8641 aaagctcatc gccgcgctgc cgcgactggc acgccagtct gtggatgacg tgcttgcagc
     8701 cgtcggtcgc ggcgagatgt attcgggcga tgtggtcaag gccgtctatc cggatttcaa
     8761 ggacgagcag gtgaagggtg cgaccccggc gcgcggcgcc ggctggttcg aactgtcgac
     8821 agccgaaagc ctgaagttca aggttccggg gcagggcaac cagacccatg ccttgccgat
     8881 ccgcggcctg accggcgaat tgccggtgcg ctttgccccc aatggcggcg cggttccggg
     8941 cgatcgcatt gttggcattc tgaccccggg cgagggcatc accgtctatc cgatccagtc
     9001 gccggcgctc agcgcgttcg aggatgagcc gcgccgctgg ctggatgtcc gctgggatct
     9061 cgacgaggcg gccaagagcc gctacccggc gcaattgcac cttgaggtca tcaacgagcc
     9121 cggtgccctc gcgtcgatca cccgcgtcat cggcgatgcc gacgccaaca tcgattcaat
     9181 ctcgatgatg agccgcacgg ccgatttccg cgagatgctg atcgatatcg aggtctggga
     9241 cctcaagcac ctcaacgcga tcatctccga actgcggacg acggcgcagg tttccaaggt
     9301 cgagcgcgtc aacgggtgag gaacggtccg gcttgccgga cgaaaagctt cagtggagct
     9361 tttcgagcga cgaacgcccc gagcccctgc gagggaccgg gagcggttca actgacccca
     9421 ccccctcgct gtcgctttga cgctccccta gagcattttc aagcgaagtg gattccggtt
     9481 cgcgttaaga aaatgcgaaa a
//
LOCUS       DAOMXG010000092         9375 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_3880_length_9375_cov_9.433691, whole genome shotgun sequence.
ACCESSION   DAOMXG010000092 DAOMXG010000000
VERSION     DAOMXG010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 9375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9375
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3880_length_9375_cov_9.433691"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..671)
                     /locus_tag="PLQ11_07505"
     CDS             complement(135..671)
                     /locus_tag="PLQ11_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HRK24783.1"
                     /translation="MANNPFLWWLPTGTPDQGAARTPAPPPPDGLPDPGPLIRRAQQI
                     AVAIFLEECAELDLTPVQHAVLATLADHPGSDATRIAGLTGFDRTTISGVMERLEAKG
                     LISRKALASDRRIKTLALTAAGRRLLARAEPLVEKVQQRILAPLPKQEQELLLILLAR
                     LVEANNGVSRAPRRGEPA"
     gene            875..1579
                     /locus_tag="PLQ11_07510"
     CDS             875..1579
                     /locus_tag="PLQ11_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRK24784.1"
                     /translation="MSGFVIAPPPVSSIAVAGSKDRFPVRRIYCVGRNYEAHAREMGK
                     DPTREAPFFFMKPADAIVDSGQTIPYPPDTANLHHEIEMVVAIGKGGVNIPEDQALAH
                     VWGYGIGIDFTRRDRQGEAKDAGRPWEWGKAFDRSAPMAPLHPVSATGHVARGRIWLA
                     VNGKVRQDQDVADLIWSVPEIISICSRSMALAPGDVIMTGTPAGVAAVVAGDVLTGGV
                     EGIGEIMITLGPKAGA"
     gene            1576..2421
                     /gene="cysQ"
                     /locus_tag="PLQ11_07515"
     CDS             1576..2421
                     /gene="cysQ"
                     /locus_tag="PLQ11_07515"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008933709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HRK24785.1"
                     /translation="MNSPSRLCRRIDAALMSALADAALEAAAVVMSYHGRAGVRAKSD
                     GSPVTEADIAAEAVIRAALARLLPDVPVLAEEETAQSGAPDVGASFVAVDALDGTKEF
                     IAAGREFTVNIGLIEAGNPVAGVIVAPALARLWLGWPGTALRAETQPGRRFQEAANQR
                     SLAVRRPAPSRLVAVSSRTHSTRQTSAFLATLPVSERRIAGSSLKFCQIAEGEADIYP
                     RFGPTMEWDTAAGHAIVLAAGGAVWAADGEDLVYGKRADGYHNPDFVAAGDRHGIEAL
                     QAGAG"
     gene            2502..4502
                     /locus_tag="PLQ11_07520"
     CDS             2502..4502
                     /locus_tag="PLQ11_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HRK24786.1"
                     /translation="MPTHPSLPLVRNAAKGKVVSAQAAVQLIRDGDTIATGGFVGIGF
                     AEEIAIAIEQRFLASRADDPQAPGRPGNLTLVYAAGQGDGKSKGLNHFGHPGMVRRVI
                     GGHWGLVPTLQKLAITNQIEAYNLPQGVIVHLFRDIAAHRPAHLTTVGMETFVDPKNG
                     GGKLNARTTEDLVTRVTIHGQECLAYKTFPIHVGIIRATTADPDGNLTMEKEALTLEA
                     LAIAMAARNSGGIVIAQVERVAESGSLNPRQVKIPGVLVDCVVVARPENHWQTFATPY
                     NPAFSGEIRVQADSIPPMPLNERKIIARRAAFELRPNAVVNLGIGMPDGVAAVANEEK
                     IIDLVTMTAEPGVIGGIPAIGLNFGAASNTQAIIDQPYQFDFYDGGGLDMAFLGLAQV
                     DREGNLNVSKFGPKLAGAGGFINISQKARQVVFVGTFTAGKLELASGDGRLAIVTEAT
                     TPKFVERVEHVTFSGRFAAENGQPVLYITERCVFRLVEGGLELIEVAPGIDIERDILR
                     LMEFRPLVPRQPALMDARIFRAAPMDLRGDLIVTPLADRYSYDPAQGTIFINFEHLTV
                     RSQADIEAIRAAAEQHLLPLGRRVPAVVNYDHFSISPELLDAYTEMVKGLEARFYTTV
                     SRFTTNAFLRMKLGEAFHQHGMAPHMFGNEVEALERSLKDRD"
     gene            complement(4486..4854)
                     /locus_tag="PLQ11_07525"
     CDS             complement(4486..4854)
                     /locus_tag="PLQ11_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020628870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HRK24787.1"
                     /translation="MPQSLVAAPSEIRVTDGGRMVVVTFADFGRFELPAEFLRVESPS
                     AEVQGHNPKEKKTVPGKRNVRITGAEPVGNYAVRLIFSDGHSSGLFTWETFADLGRNR
                     QERWNSYLANLAELGLSRDP"
     gene            5189..5581
                     /locus_tag="PLQ11_07530"
     CDS             5189..5581
                     /locus_tag="PLQ11_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HRK24788.1"
                     /translation="MKHVVVKMLAGAAFVAVSATGAFAQDVAAGEKSFNKCRPCHQVG
                     ETAKNIVGPKMNGLFGRKSGTVEGFAYSDPNKNSGITWDEAVFAEYIKDPKAKIPGTK
                     MVFAGIKDEQEIKDLTAFLKQFKADGTK"
     gene            5690..6799
                     /locus_tag="PLQ11_07535"
     CDS             5690..6799
                     /locus_tag="PLQ11_07535"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HRK24789.1"
                     /translation="MADRLIKAVGVMSGTSMDAIDVALVETDGEAMCRFGPVRAYPYE
                     AALRAELQGLIADPARAADGDCAALEAAVTDAHCAAVEAFLDQTGQSREAVGIVGFHG
                     QTVFHAPARRLTRQLLDGPRAARRLGIDVVYRFRHADVAAGGQGAPLVPLFHRALATG
                     LACPLAVLNLGGVANISYIDGETVLACDTGPASALIDDFMLRRRGEPFDRDGALAASG
                     QVEQATLARLLAHPFIAASLPKSLDRNAFHALAAAVDGLSDADGVATLTAFTVEATAA
                     ILPHLPAAPLRWLVTGGGRHNATLMQRFAARLGVPVVPVEAVGWDGDALEAQCFAWLA
                     VRSLKGLPLSLPTTTGVPRPMPGGEYVPVGVSSPA"
     gene            6880..7881
                     /locus_tag="PLQ11_07540"
     CDS             6880..7881
                     /locus_tag="PLQ11_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009494558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HRK24790.1"
                     /translation="MTIRFATFAAVLALAAGPALAQTAIKPAVTFDVGGKFDKSFNEA
                     SYTGAEAFKKKTGIAYREFEVQNDAQREQAFRRFARDGQDPIIATGFSNGAALKKVAE
                     EFPKTRFTIIDFVIDLPNVQSIVFKEHEGSYLVGLLAAKASKSGKVGFVGGMDIPLIR
                     KFACGYVQGVKAGNPAAEIIQNMTGTTGAAFNDPVKGGELAKSQMDRGADVIYHAAGA
                     TGIGVLRAVADAGKLGIGVDSNQNALQPGKVLTSMLKRVEVAVENTLMQAHEGKWKPG
                     VTVLGLAEGGVAWALDDHNKGLITDAMKAAAAKAEADIKSGAIKVHDYMSDQKCPVQ"
     gene            7958..8695
                     /locus_tag="PLQ11_07545"
     CDS             7958..8695
                     /locus_tag="PLQ11_07545"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRK24791.1"
                     /translation="MQLTILGSGDAFCSGGRFNTCLKLDGPGGSMLVDCGATTMLALN
                     RAGIDRNAIDTILVTHFHGDHFAGLPFFILDARFVSRRRTPLRIAGPKGLAERCRAIV
                     ETTFPGFWDGPQDFPIETGEISPGAPLTLCGMAVTAFPAVHDERAGPCQSYRFEADGK
                     VLAFSGDTSWTEALVPLAAGADVFLCECYTPATRIASHLDWQTIADNRARLTARRLIL
                     THMGPPMLAHAGPLDAERAFDGMVVTL"
     gene            8692..>9375
                     /locus_tag="PLQ11_07550"
     CDS             8692..>9375
                     /locus_tag="PLQ11_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011423581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRK24792.1"
                     /translation="MSDLAIELAGIDKRFGAVHANRAIDLAVRKGSIHGIIGENGAGK
                     STLMSILYGFYEADKGDIRIDGRPVRIRSSADAIAAGIGMVHQHFMLVEPMTVAENVL
                     LGAEGAALIDRGLDRAREEIRRLGRDYGLVVDPDAVVGELSVGLQQRVEIIKALYRGA
                     NILILDEPTGVLTPAEASQLFEVLAELKRQGRTILIITHKLKEIMSITDHVSVMRRGA
                     MVAHRSTSET"
BASE COUNT         1617 a         2961 c         3068 g         1729 t
ORIGIN      
        1 tgatccgatc tggtcggatt ttgctctagt gagctatgca ccgaggttca acggacaaac
       61 gctgaggatg acgagccgca ccggcaaacg atcccccgac atggcttgca gatctcccca
      121 gcgcgttcat cgactcatgc gggctcgcct cgccggggtg cgcgcgatac gccattgttg
      181 gcctcgacca ggcgcgccaa tagaatcagc aacagctcct gctcctgctt gggcagcggg
      241 gccaggatgc gttgctggac tttctcaacg agaggctcgg cgcgggccag cagcctgcgg
      301 cccgccgcgg tcaaggccag ggtcttgatc cgcctgtcgc tggccagcgc cttgcggctg
      361 atcaggccct tggcctccag ccgttccatc accccgctga tcgtcgtgcg atcaaatccc
      421 gtcagcccgg cgatccgtgt tgcatcgctg ccagggtgat cggcgagtgt ggccagcacc
      481 gcatgctgga ccggagtgag gtcgagttcc gcacattcct caaggaaaat cgccacggcg
      541 atctgttgcg cgcgccggat cagcggcccc ggatccggca ggccatccgg cggcggcgga
      601 gccggtgttc tggctgcgcc ttgatcggga gtgccggtcg gcagccacca caggaacgga
      661 ttgttcgcca tctgtctctc ccaccggttg gagcaagggt ctcgattgtt gatcagcata
      721 ccacgcattt tcgcaaatgc acggcatggt tgctgcgagc gatcgggggg aagggcagga
      781 caagaatcgc tttacaacgt ccaggaattg gccaagcttg tctgacaacg gggctgccgc
      841 catcgcctgc cgccccccgc gtccagaggc tttcatgtca gggtttgtca ttgctccgcc
      901 gccggtatcc tcgatcgctg tggcaggttc gaaggatcgg ttcccggtcc gcaggatcta
      961 ttgcgtcggt cgcaactacg aagcccacgc ccgcgagatg ggcaaggatc cgacgcgcga
     1021 ggcgccgttc tttttcatga agccggcgga tgcgattgtc gacagcggcc agaccattcc
     1081 ctatccgccc gataccgcca atctgcatca cgagatcgag atggtggtgg caatcggcaa
     1141 gggcggtgtc aacatccccg aggatcaggc actggcccat gtctggggct atggcatcgg
     1201 catcgatttc acccgccggg accggcaggg cgaggccaag gatgccggcc ggccgtggga
     1261 atggggcaag gcgtttgacc ggtccgcgcc gatggcgccg ctgcatcccg tctctgccac
     1321 tggccatgtc gcccgcgggc gcatctggct ggccgtcaat ggcaaggtgc ggcaggatca
     1381 ggacgtggcc gatctgatct ggtcggtgcc ggagatcatc tcgatctgct cgcggtccat
     1441 ggcgctggcg ccgggcgatg tgatcatgac cggcacgccg gcgggggtcg cggccgtggt
     1501 cgcgggggat gtgctgaccg gcggcgttga ggggattggc gagatcatga tcacgctggg
     1561 gcccaaggcg ggcgcatgaa ttctccctcg cgtctttgcc gacgcattga tgccgcactg
     1621 atgtccgcac ttgccgatgc ggcgctggag gccgcagccg tggtgatgtc ctaccacgga
     1681 cgcgccggcg ttcgggcaaa atccgacggc agcccggtga ccgaggcaga catcgccgcc
     1741 gaagcggtga tccgcgccgc gcttgcgcgg ctgttgccgg atgttccggt gctggcggag
     1801 gaagagactg cgcaatcggg cgcgccggat gtaggggcct cttttgttgc ggtcgatgcg
     1861 ctcgatggca ccaaggaatt catcgcggct ggcagggaat tcaccgtcaa catcgggctg
     1921 atcgaagcgg gcaatcctgt cgcgggtgtg attgtcgcgc cggcgctcgc acgcctgtgg
     1981 ctcggctggc cgggaacggc cttgcgggcg gagacccagc cgggcaggcg ctttcaggaa
     2041 gcggcaaacc agcgctccct ggcggtgcgc cgtcctgctc cgtcacggct ggtggcggtg
     2101 tcgagccgca cgcacagcac ccgccagacg agtgcctttc tggccaccct gccggtatcc
     2161 gaacggcgca ttgccggttc ctcgctgaaa ttctgccaga tcgccgaggg tgaggcggat
     2221 atctatccgc ggttcggtcc gaccatggaa tgggatacgg ctgctggtca cgccatcgtg
     2281 ctggcggcgg ggggggcggt ctgggctgcg gatggtgagg atcttgttta tggcaagcgt
     2341 gcggatggct atcacaaccc ggattttgtc gccgcgggcg accggcacgg gatcgaggcg
     2401 cttcaagccg gcgccggatg agccggccgt gcggcaagcc cgttgacgct ggcatcgttc
     2461 tgagtggaaa tactgcctga tctgccgaga cgggagctcg catgccaacc catccatccc
     2521 tgccgctggt ccgcaacgct gccaagggca aggtggtctc cgcgcaagct gcggtccagt
     2581 tgatccgcga cggggatacc atcgctaccg gcggctttgt cggcatcgga tttgccgagg
     2641 aaatcgccat tgcgatcgaa cagcggtttc tggccagcag ggcggatgat ccgcaggcac
     2701 cggggcggcc gggtaacctg acgctggtct atgcggccgg gcagggcgac ggcaaatcca
     2761 agggcctcaa ccacttcggc catcccggca tggtgcgccg ggtgatcggc gggcattggg
     2821 ggctggtgcc aacccttcag aagctggcca tcaccaacca gattgaagct tataacctgc
     2881 cgcagggcgt gattgttcac ctgttccgtg acatcgcggc gcatcgtccg gcccacctga
     2941 ccacggtcgg catggaaacc ttcgtcgatc cgaagaacgg cggcggcaag ctcaacgccc
     3001 gcaccaccga agatctggtg acgcgcgtca ccatccacgg ccaggagtgt cttgcctaca
     3061 agaccttccc gatccatgtc ggcatcatcc gcgccaccac cgccgacccg gacggcaacc
     3121 tgaccatgga aaaggaggcg ctgacgctgg aggcgctggc cattgccatg gccgcgcgca
     3181 attccggcgg cattgtcatt gcccaggtcg agcgtgttgc cgagagtggc agcctcaacc
     3241 cgcggcaggt gaagattccc ggcgtcctgg tcgattgcgt tgtcgtcgcc cggccggaaa
     3301 accactggca gaccttcgca acgccctaca atccggcctt ttccggcgaa atccgcgtgc
     3361 aggcggattc cattccgccg atgccgctca acgagcgcaa gatcatcgcc cgtcgcgctg
     3421 cattcgaact gcgccccaac gcggtggtca atctcggcat cggcatgccc gacggcgttg
     3481 cggccgtcgc caacgaggag aagatcatcg atctggtcac catgacggcg gagccggggg
     3541 tgatcggcgg cattcctgcg atcggcctga atttcggcgc cgcctcgaac acgcaggcga
     3601 tcatcgacca gccctaccag ttcgatttct acgacggcgg cgggctcgac atggcttttc
     3661 tcggtctcgc ccaggttgac cgcgagggca atctcaacgt ttccaaattc ggccccaaac
     3721 tcgccggcgc gggcggcttc atcaacatct cgcagaaggc gcggcaggtg gtgttcgtcg
     3781 gcacattcac cgccggcaag ctggagcttg catccggcga cggccggctg gcgattgtca
     3841 ccgaggcgac gacgccgaaa ttcgtcgagc gtgtcgagca tgtcaccttt tccggtcgct
     3901 ttgccgccga gaacggccag ccggtgctct acatcaccga gcgttgcgtg ttccgtctgg
     3961 tcgagggcgg gctggagctg atcgaggtcg ctcctggtat cgacatcgag cgcgacatcc
     4021 tgcgcctgat ggagttccgt ccgctggttc cgcgccagcc tgccttgatg gatgcccgca
     4081 ttttccgggc cgcgccgatg gatctgcgcg gcgatctcat cgtcacaccg ctggcggatc
     4141 gttacagcta cgatccggcg cagggcacga tcttcatcaa cttcgaacat cttaccgtgc
     4201 gctcgcaggc ggatatcgag gccattcgcg ctgcagccga gcagcatctg ctgccgctgg
     4261 gccggcgcgt tcccgcagtg gtcaattacg accacttttc catctcgccc gaattgctcg
     4321 atgcctatac cgagatggtc aagggtctgg aggcgcggtt ctacacaacc gtttcgcggt
     4381 tcactaccaa tgccttcctg cggatgaaac tgggcgaagc cttccatcag cacggcatgg
     4441 cgccgcatat gttcggcaac gaagtcgagg cgctggaacg tagcctcaag gatcgcgact
     4501 gaggccgagc tctgccagat tggcaaggta ggaattccag cgctcctggc ggttgcggcc
     4561 aagatccgcg aaggtttccc aggtgaacag accgctggaa tgcccgtccg agaagatcag
     4621 ccgcacggca taattgccca ccggctcggc cccggtgatg cgcacattgc gcttgcccgg
     4681 aaccgtcttc ttttccttcg gattgtgccc ctgaacctcc gccgaagggc tttccacccg
     4741 caaaaactcg gccggaagct cgaatcggcc gaaatcggca aaggtcacga caaccattcg
     4801 gccgccatcc gtgacgcgga tttcactggg ggcggcaaca agggattggg gcatcgggcg
     4861 aggttccggc aacgggctga ctgccatccg gttgttccgg cggcgggcgt cgatgtcaag
     4921 tcttgcccgt tcctgcgccg cgtcgaattg ccctgtcgcg cctctcgcgc cagaatgccg
     4981 gatcggctag cacactggac cttgcgcgga tgggtcggac ggataagccg gtgcggaacg
     5041 cgcacgctgg acatctgcca ggattgccgg cgccttcgcc gaaagactga atgaagagca
     5101 cgccccttcc agtcatgttc acatttggta tgtgataggg gtatgcgcca ttcggttgga
     5161 ccgacaacgc aagaaaggga tgaatgagat gaagcatgta gttgtaaaga tgctggctgg
     5221 tgcggctttc gtggcggttt cggccaccgg tgccttcgcc caggacgtcg cggcaggtga
     5281 gaagtcgttc aacaagtgcc gcccctgcca tcaggtcggt gagactgcca agaacatcgt
     5341 tggcccgaag atgaacggcc tgttcggccg caagtccggc acggtggaag gcttcgccta
     5401 ttcggatccg aacaagaact ccggcatcac ctgggatgag gccgtgttcg ccgagtacat
     5461 caaggatccg aaggccaaga tccccggcac caagatggtg ttcgccggca tcaaggacga
     5521 gcaggagatc aaggatctga ccgccttcct caagcagttc aaggctgacg gcaccaagta
     5581 agccgctgcc ccccgtcagg attcggcgcg ctggcatgcg aaagagcatg ccggcgcgtt
     5641 ttcgtttatc tgttgacgga gtgattcgct ttcggacctg gagctgactg tggctgaccg
     5701 gctgatcaag gccgttggcg tgatgtccgg aacctcgatg gatgccatcg acgtggcgtt
     5761 ggtcgagacc gacggcgagg ccatgtgccg gttcggcccg gtccgcgcct acccctatga
     5821 agcggcgctg cgggcagagc ttcagggcct gattgccgat ccggcgcgcg ctgccgacgg
     5881 ggattgtgcg gctctggaag ctgcggtcac cgatgcccat tgcgcggcgg tagaagcctt
     5941 tcttgaccag accggccagt cgcgcgaggc tgtcggcatc gtcggctttc atggccagac
     6001 ggtgtttcac gcgcccgccc ggcggctgac gcggcagttg ctggatggtc cacgcgccgc
     6061 ccggcgcctc ggcattgatg tggtctatcg cttccgccat gccgatgtcg cggcaggcgg
     6121 gcagggggcg ccgctggtgc cgctgttcca tcgggcgctg gcaaccggac ttgcctgtcc
     6181 gctggcggta ctcaacctcg gcggtgtcgc caacatttcc tatatcgacg gcgagacggt
     6241 gctggcctgc gacaccggcc cggcgagcgc gctgatcgac gatttcatgc tgcggcgccg
     6301 gggtgaaccc ttcgaccgcg atggcgcgct tgccgcttcc ggccaagttg aacaggcaac
     6361 gctggcgcgg ctgctggcgc acccgtttat tgccgcgtcc ctgcccaagt cgctcgaccg
     6421 caacgccttt catgcgcttg ccgccgccgt ggatggcctc agcgatgccg atggtgttgc
     6481 gacattgacg gcgttcacgg tggaggcgac agccgccatc ctgccgcatc ttccagcggc
     6541 tccgctgcgc tggctggtca ccggcggcgg ccggcacaac gcaacgctga tgcagcgctt
     6601 tgccgcgcgg cttggtgtgc cggtggtccc tgtcgaggcg gtcggctggg acggtgatgc
     6661 gctggaagcg caatgctttg cctggctggc ggtgcggtcg ctgaaggggc ttccgctcag
     6721 ccttccgacg accaccgggg tgccgcgtcc gatgccgggc ggagagtacg taccggttgg
     6781 cgtatcgtcc ccagcctgaa gacaagccca ctggactctg cgggccgtcg cggccatgct
     6841 gcagcaagtc tgcctcacga gcggaccagg gagacataca tgaccatccg atttgctact
     6901 ttcgcagccg tgctggcgct tgccgccggc cccgcgctgg cccagaccgc gatcaagccc
     6961 gccgtgacct tcgatgtcgg cggcaagttc gacaagtcgt tcaacgaggc gagctacacc
     7021 ggcgccgaag ccttcaagaa gaagaccggc atcgcctacc gtgaattcga ggtgcagaac
     7081 gatgcccagc gcgaacaggc gttccgtcgc tttgcgcgcg acgggcagga tcccatcatc
     7141 gccaccggct tttccaacgg cgcagcgctg aagaaggttg ccgaggaatt tccgaaaacc
     7201 cgcttcacca tcatcgattt tgtcattgac ctgccgaacg tgcagtcgat cgtcttcaag
     7261 gagcatgaag gcagctatct cgtcggtctg ctggccgcca aggcgtccaa gtccggcaag
     7321 gtcggcttcg tcggcggcat ggatattccg ctgatccgca aattcgcctg cggttacgtg
     7381 cagggcgtca aggccggcaa tccggcggcc gagatcatcc agaacatgac cggcacgacc
     7441 ggcgcggcct tcaacgatcc ggtcaagggc ggcgaactgg cgaaaagcca gatggatcgc
     7501 ggcgctgacg tgatctacca cgcagccggc gccaccggca ttggcgttct gcgcgccgtg
     7561 gccgatgccg gcaagctcgg aatcggcgtt gattccaacc agaacgccct gcaacccggc
     7621 aaagtcctga cctccatgct caagcgcgtc gaggtggcgg tcgagaacac gttgatgcag
     7681 gcgcatgagg gcaagtggaa gcccggcgtc accgtgctcg gccttgccga aggcggtgtt
     7741 gcctgggcgc tcgacgatca caacaagggg ttgatcaccg atgcaatgaa agccgctgcg
     7801 gccaaggcag aagccgacat caagtccggc gccatcaagg tgcacgacta catgtcggat
     7861 cagaagtgcc ctgtgcaata agacacagaa gccggagcaa tggcatgatg gaggtccggg
     7921 cgcgcgtcct gcgcccggat cgcaagtgga acaggcgatg caactcacca ttctcggctc
     7981 gggcgatgcc ttctgttccg gtgggcggtt caacacctgc ctgaagctgg acgggcctgg
     8041 cgggtcgatg ctggtggatt gcggcgcgac cacgatgctg gcgctcaatc gtgccgggat
     8101 tgaccgcaat gccatcgaca cgattctggt gacgcatttc cacggcgatc atttcgccgg
     8161 cctgccgttt ttcattctcg atgcgcgctt tgtcagccgc cgccgcacgc cgctcaggat
     8221 cgcagggccc aaaggtcttg ccgagcgctg ccgcgccatc gtcgagacga cttttcccgg
     8281 gttttgggat ggcccgcagg atttcccgat cgagaccggg gaaatttccc caggagcgcc
     8341 actcaccctg tgcggcatgg cagtgacggc attcccggcc gtgcatgacg aacgcgccgg
     8401 tccgtgccag agctatcggt tcgaggcgga cggcaaggtg ctggccttct ccggcgatac
     8461 ttcatggacc gaggctctgg ttccgctggc cgccggcgcc gatgtatttc tgtgcgaatg
     8521 ttacacgccg gcgacgcgga tcgccagcca tctcgactgg cagacgattg ccgacaaccg
     8581 cgcgcggctg accgcccggc ggctgatcct cacccatatg gggccgccga tgctggccca
     8641 tgccggcccg ctggacgccg aacgcgcctt cgacggcatg gtggtgacgc tatgagcgat
     8701 cttgccatcg aacttgccgg aatcgacaag cgctttggtg cggttcatgc caacagggcg
     8761 attgatctgg cggtgcgcaa aggttcgatc cacggcatca tcggcgagaa cggggccggc
     8821 aaatcgacgc tgatgtcgat cctctacggc ttctacgagg cggacaaggg ggatatccgc
     8881 atcgacgggc ggccggtgcg catccgctcc tcggccgatg cgattgctgc ggggatcggc
     8941 atggtccatc agcatttcat gctggtcgag ccgatgacgg tggctgaaaa cgtcctgctt
     9001 ggcgccgagg gcgctgcgct gatcgaccgt ggactcgaca gggcgcgcga ggaaatccgc
     9061 cgcctcggcc gcgactacgg ccttgtggtc gatcccgatg cggttgtcgg cgaattgtcc
     9121 gtcggcctgc agcaacgcgt cgagatcatc aaggcgctgt atcgcggcgc caatatccta
     9181 attctcgacg agccgaccgg cgtgctcacc ccggccgagg ccagtcagct gttcgaggtt
     9241 ctggccgaat tgaaacggca gggccggacc atcctcatca tcacccacaa gctcaaggag
     9301 atcatgtcga tcaccgatca tgtctcggtg atgcggcggg gcgcgatggt ggcgcatcgc
     9361 tcgacctccg aaacc
//
LOCUS       DAOMXG010000093         9318 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_3910_length_9318_cov_11.059484, whole genome shotgun sequence.
ACCESSION   DAOMXG010000093 DAOMXG010000000
VERSION     DAOMXG010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 9318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9318
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3910_length_9318_cov_11.059484"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..419)
                     /locus_tag="PLQ11_07555"
     CDS             complement(156..419)
                     /locus_tag="PLQ11_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24793.1"
                     /translation="MLRSVALVTFIVMPTAAVFAEGTSRFPERRGAVQPKLPPCECRA
                     EGRRWGEGQQVCLDLTGKRRMYVCAVQQNVTTWMETNPTCPDS"
     gene            444..842
                     /locus_tag="PLQ11_07560"
     CDS             444..842
                     /locus_tag="PLQ11_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24794.1"
                     /translation="MLLFGRLEVKDKGPARRCLKVSAGSLRFYLRAPKMVRTGKEQAM
                     KVSVILAVLAFCATGLSAEAGAPTLVAQGKTCSSFQTMCANRCRMRAPQDANCVDDHC
                     TPKLNTCRQDGCWQEGRLYGGRKTCGLVKS"
     gene            complement(861..1229)
                     /locus_tag="PLQ11_07565"
     CDS             complement(861..1229)
                     /locus_tag="PLQ11_07565"
                     /inference="COORDINATES: protein motif:HMM:NF021447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HRK24795.1"
                     /translation="MSRHLARFRAAALAGTMLILPAAAQQAAPPVAVPALPAAPPAAV
                     LAPAADAKVIVVGSVVQLRSGGPKLTVVDLAPTVATVQWYVDNSFKKESFPIAALKLV
                     DASDDDDEEEDDDEDEDEDE"
     gene            1505..2542
                     /locus_tag="PLQ11_07570"
     CDS             1505..2542
                     /locus_tag="PLQ11_07570"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018097956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HRK24796.1"
                     /translation="MAGAALETLATGLANPWALAFLPDGRMLVTERPGRMRLVSSNGA
                     VSAPLAGVPAVWARGQGGLLDLCLAPDFATSRTLYFSYAEPRDGGAATSVARARLSAD
                     ATRLDDVKVIFRQQPASGGPVHFGSRIVAGRDGTLFVGLGERGQMDRAQDLSTHYGKV
                     VRIHTDGAAPRDNPFIGKPGILPEIWSVGHRNIQGAALHPETGALWTVEHGAMGGDEI
                     NIPEPGRNYGWPVITYGRDYSGAKIGIGPRKDGMEQPIHYWDPSIAPSGMMFYTGDAF
                     PHWRGSLFIGALAGSLVARLTVENNRIVAEERLLAGLGHRYRDIRQGPDGFINLLTDA
                     ADGLILRLRPA"
     gene            complement(2873..4123)
                     /locus_tag="PLQ11_07575"
     CDS             complement(2873..4123)
                     /locus_tag="PLQ11_07575"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HRK24797.1"
                     /translation="MLRALDLCEALEAGDLAATTIVEWIAEAVAEREAGLSTFAHLDL
                     DRLRLEARQPASGPLGGLPVAFKDIIDTADAPTEYGSPIYHGWRPRADAPIVCQTRAA
                     GGLMLGKAVTTEFAFLEPAITRNPHDPARTPGGSSSGSAAGVAAGLMPLAFGTQTGGS
                     VIRPASFCGVAAIKPSYRLLPMVGIKPGAWTLDTLGLFGARVADIAFGLSAITGRDLR
                     VDGRDFGTPTFGVTRMPFAGAAAPEAEAALVRAIAAVEKSGGRIVEVDFGAPVVEAQA
                     AHATIYNYEGGVALAWEIAHHRDRLSAILKQKFAPEVPITIADYDHARGIAKRARRAA
                     RELFAGFDALITFAAPGAAPDRSTTGDAAFNKLFTLLGTPAVNVPGCKCSVNMPVGLQ
                     VVAAFGQDHRALAAAHFLEQALNS"
     gene            4207..4848
                     /locus_tag="PLQ11_07580"
     CDS             4207..4848
                     /locus_tag="PLQ11_07580"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRK24798.1"
                     /translation="MDRVCPKGPLGFFAARKARDSYHHGHLKLTLLDTAETLIAERGP
                     ANLSLSEMARMAGVSSAAVYRHYADLNALIGAVAHRGFEDFAARLKAGAAVAGSGVDG
                     LRGMGRAYLDFARERPGAYAAMFSSTVSYTDPGLAAAGETAFNTLIQGVSATLSKSGM
                     PAEAAYGLALKIWALAHGVATLAGSGRLTPAVGIDPATLLSEAVTALLSAESR"
     gene            4971..6731
                     /locus_tag="PLQ11_07585"
     CDS             4971..6731
                     /locus_tag="PLQ11_07585"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRK24799.1"
                     /translation="MASTGLRLHRGIIAFVDVVESVRLIAEDGGAFIARWLHQRSGVE
                     AILQQHNGRLVRSTGDGLIGEFADARAALKAAFETQARLASANAGAERPVVVRIGLSK
                     GTYYADDKDIYGEAVNLAARLTGLGQPDSVIASDAVAADLTDGIDAVIEDLGACFLKH
                     IDKPVRAYALRPVGSIARAPALARHPVSLVPTIAILPLVRLHGEGASDALGDIIADTL
                     IADLSGSPYLNVISRMSATMVSASRLDPTKAAQALGADYIVSGSYALSFGRMRTTLEL
                     MERKSGLVLIEERFSLPADALVAPDFRGFESFTARLLHAVLRSEVQRLKRQTLPNLES
                     HSLLLGAVGMMHRPQEDDFFFAANVLQTLMDRHPREGLPAAWLACWYTIKVQKGFSTD
                     PEKDGLQARELARRALDLEADTPLALAVAGLIETNFGRDFEAASQLLECAANLNPNDT
                     FALLHKAALLTFTDRGEESYQISRKARLLSPVDPHRYYFEAIAASAAFSAGKYPEAIT
                     HADEAIRANRLYPSPSRVRTSALFHLGQHDEARAAAKALLRVDPELTVHRWLRQSPAA
                     DYDVGRRLAEGLKGAGIPDS"
     gene            6752..7972
                     /locus_tag="PLQ11_07590"
     CDS             6752..7972
                     /locus_tag="PLQ11_07590"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRK24800.1"
                     /translation="MTFMNGLNGLNGLNGLNGLNGLNGLNGLNGLNGLNGLNGLNGLN
                     GLNGLNGLSGSLSGGPGSILTTHDAIAGLYQPPAHAPTAIPAAARLDLGRWSADVRAR
                     ILDADLFPRLAFGKKPGAPDQFMVLAGQQTWSTTGAAPILVQQYDPLFTINPPTIASL
                     VSQQLPLVANAAVLRGDRLHEIIDQIVLPIGFYGAIVPLSPERKRWTLELMMVAFRLS
                     VFVEMAFKHAFSLPRPNEMSPQINPVIPTPLHATLPSGHATENFAMATILWELLRRSP
                     APERDPAYRDRLFKLADRIAYNRTVAGVHFPVDNAAGAALGTTLGLFLVDQCLDGAGG
                     GCHEWAFDAAVFCNPAQHDGFFDYADLVGQMLPGGGGAAATPVPGLSQMNGLAFPRSA
                     LLGALWSKALDEWS"
     gene            8013..>9318
                     /locus_tag="PLQ11_07595"
     CDS             8013..>9318
                     /locus_tag="PLQ11_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24801.1"
                     /translation="MLWTTGTSAYQGLPLAMDPLADWIRPENRPADHRILVLIRLAAG
                     ITAQDLPDFPGSTETGPVFVFKGKPVTHRRWQQALAVPRCYTGARGPWNRARYCTALA
                     TPDFFQWLLAEPKVQALFDEITLCITPDVSEPPADDREAGVAIDYSLPEAKVVTGVID
                     YGLPLAIPLYSGIAPDGATVAPPRLLSVWVQDGSPVRASAHNGLSRPTEFRYGAELSA
                     TAIRRIMRRAGGDEDRFYRMAALFVPARLGTYWTQHTTTHGAHVLGNVVDPAADAGHN
                     PVIAVHLPSVAVEDRTGASLSGHAVDAMRFIIARTEDLSDPEDGRPMPPLVINLSYGF
                     HAGPHDGNAPIEKAMTELHALWSGVYPAAPFVIVVPAGNAYQSSTHACWTASMLASGD
                     ASVTWEIMPDDASCNILQIWLPEGLAGGATARLTSPQGQTVQL"
BASE COUNT         1654 a         3061 c         2905 g         1698 t
ORIGIN      
        1 cttggcccga aactggtcat cgcaacgatt caatctgatc ggaaagtgct ctaagtggat
       61 accggtcaat cgcagaagac atgctctaac ttattgatac cgatcacgtt atccgcattt
      121 tgatgattcc atcaaaatgc ggcgtgatct aattctcagc tatcagggca ggttggattg
      181 gtttccatcc aggtcgtgac gttctgctgc acggcacaga catacatccg ccgcttgccg
      241 gtcagatcga ggcagacttg ctggccttct ccccagcggc gaccttcggc gcggcactcg
      301 cagggcggca gcttgggctg cacggctccg cgccgttcag gaaagcgcga cgtcccctcc
      361 gcaaaaactg cggcagtcgg catgacgatg aaggtgacaa gtgcaacgga acgcagcatg
      421 gcagtctcca ttggacacgc cggatgctgc tcttcgggcg ccttgaagtc aaggacaagg
      481 ggccagcgcg gcgctgcctt aaagtcagtg ccggcagctt gcgattttac ctgcgcgcgc
      541 ccaagatggt cagaacgggg aaggagcaag cgatgaaggt atcggtcata ctggcggtgt
      601 tggctttttg cgccacaggt ctgtcagccg aggctggagc accaacactt gtggcgcaag
      661 gcaagacctg ttcgagtttc cagacgatgt gcgccaaccg ttgccgcatg cgcgcgccgc
      721 aggatgccaa ttgcgtcgat gatcactgca cgcccaaact gaacacctgc cggcaggacg
      781 gctgctggca ggaaggcagg ctctacggcg gcaggaaaac ctgcggactg gtcaagagtt
      841 gagcgcgacg atgcgcctgc ctattcgtcc tcgtcctcat cctcgtcgtc gtcctcttcc
      901 tcgtcatcat catcggacgc atcaacgagc ttgagggcgg cgatcgggaa gctctccttc
      961 ttgaaactgt tgtcgacata ccattgtacg gtcgcaacgg tcggcgcgag gtccacgacg
     1021 gtcagcttcg gaccaccgga gcgcaattgc accacagaac cgaccacgat caccttggcg
     1081 tcagccgcag gcgccagaac ggcggccgga ggcgcagccg gcaaagcggg aaccgcgaca
     1141 gggggagccg cctgctgggc tgcggcggga aggatcagca tggtgccggc gagtgccgcc
     1201 gcgcggaatc gggcaagatg tcgggacatg agcgcctcct gggcctgatt tcggatgcat
     1261 gaaccgtcag cagggtatgc acagtcagca tattcgtcca cgtccacgcg cttttttcct
     1321 taacgtgacc ggcaaccggg gcggtttgcc gcagaagcgc agccgtgttt gcatgccgcg
     1381 cgataagggc taccgtccca tcggcaaccg gaaactcccc atgtccctca cgcgccgaac
     1441 cagccttggc ctgctcaccc tcgctctcgc tcgccctgct ctggcgcaac ggccggcgcg
     1501 cgtcatggcc ggcgcggctc tggaaacgct cgccaccggt cttgccaacc cctgggcgct
     1561 ggctttcctg cccgatggcc gcatgctggt caccgaacgg ccgggccgga tgcgacttgt
     1621 ctcgagcaat ggcgctgtct ctgcgccgct cgccggcgtc cctgcggtct gggcacgcgg
     1681 ccagggtggc ctgctcgatc tctgccttgc gcccgatttt gccaccagcc ggacgctcta
     1741 cttcagctat gccgagccgc gtgacggcgg tgccgccacc tctgtcgccc gagcccggct
     1801 atccgccgat gcgacgcgcc ttgacgatgt gaaggtcatt tttcgccagc agccggcctc
     1861 cggcggcccg gtgcatttcg gctcgcgcat cgttgcggga cgcgatggca cgctctttgt
     1921 cggcctgggc gaacgcggcc agatggaccg ggcgcaggat ctgtccaccc actacggcaa
     1981 ggtcgtgcgc atccacaccg atggcgccgc tccgcgcgac aatcccttca tcggcaagcc
     2041 gggcatcctg ccggaaatct ggtccgtcgg ccatcgcaac atccagggcg cggcgctgca
     2101 cccggagacc ggcgcgctct ggaccgtgga acacggtgcc atgggcggcg acgagatcaa
     2161 cattcccgag cccggccgca actacggctg gccggtcatc acctatggcc gcgattactc
     2221 cggcgcgaag attggcatcg ggccccgcaa ggacggcatg gaacagccga tccattactg
     2281 ggatccgtcc atcgccccct ccggcatgat gttttacacc ggcgacgctt ttccgcactg
     2341 gcgcggcagc ctgttcatcg gggcgctggc ggggtcactg gtcgccaggc tcaccgtcga
     2401 gaacaaccgg atcgtcgcgg aggaacgcct gctcgctggc ctcggccacc gctatcgcga
     2461 cataaggcag ggcccggacg gattcatcaa tctcttaacc gacgcggccg atggcctgat
     2521 cctgcgcctc aggccggcgt gacatcatcg ttttgccaaa acaaaggccg cccgaaggcg
     2581 gcccaatcac ccatgcaaca ggccaaccgg cccggagact acaatgcgcc gatgagaatg
     2641 gcagcaagaa caccgaacga cgcgatcaac gccaaaagat ccagtctcaa ggtcagaccc
     2701 atggaaaagc tccttcgtct gttgcctgac ccgcatgtta gcaaaaaatt tacgtttgtg
     2761 aagagcggcg ccagagccca tgcacaaggc cacatgaaaa aatataatat gcttaaaatt
     2821 catatacttg gaaacataaa acaagcatag ataaaatttt atgtgaattt tatcaactat
     2881 tcagcgcctg ttccagaaaa tgcgctgccg caagcgcccg atgatcttgc ccgaaggctg
     2941 cgacaacctg caatccgacc ggcatattaa cggagcattt gcatcccggc acattgacag
     3001 ccggcgttcc aagcagggtg aaaagcttgt tgaacgctgc atccccggtt gtgctgcgat
     3061 ccggcgcggc gcccggcgcg gcaaaggtga tcagcgcatc gaaacctgcg aacagctcac
     3121 gggcggcccg ccgggcacgt ttggcaatgc cgcgcgcatg atcataatcg gcaatcgtga
     3181 tcggcacctc cggcgcgaat ttctgcttca ggatggccga cagccggtcg cggtgatgag
     3241 cgatctccca ggccagggcc accccgccct cgtagttgta gatcgttgca tgggcagcct
     3301 gcgcctcgac gacgggagcg ccgaaatcca cctcgacgat gcggccgccg gacttttcaa
     3361 ccgccgcgat cgcccgcacc agcgcggcct ctgcctcggg cgccgccgca ccggcaaacg
     3421 gcatgcgcgt gaccccgaag gtcggcgtgc cgaaatcccg tccgtccacc ctgaggtcac
     3481 gccccgtgat tgccgacagg ccgaaggcga tatcggcaac gcgcgcaccg aacaacccga
     3541 gtgtatcgag cgtccaggcg cccggcttga tgccgaccat aggcagcagc cgataggacg
     3601 gcttgatcgc cgcgacgccg cagaagctgg ccgggcgaat caccgacccg cctgtctggg
     3661 tgccgaaggc cagcggcatc aatccggccg cgaccccggc agccgaaccg gacgaggaac
     3721 cgcccggcgt gcgcgccgga tcgtgcggat tgcgggtgat cgccggttcg agaaaggcaa
     3781 actcggtggt gaccgccttg cccagcatca gcccgccggc ggcgcgtgtc tggcagacga
     3841 tcggcgcatc ggcccgggga cgccagccgt gatagatggg cgagccgtat tcggtcggcg
     3901 catcggcggt atcgataatg tccttgaagg ccaccggcag gccgccgagc ggccccgacg
     3961 ccggttgccg cgcttcgagg cgcaaccggt cgagatcgag atgggcgaaa gtgctgagac
     4021 cggcctcgcg ttccgctacc gcctcggcaa tccattccac gatggtggtc gcagccaggt
     4081 cgcctgcttc gagggcttcg cagagatcaa gcgcacgcag catgatggcc cctgactgtc
     4141 ggggttgcgc ccccgcgatc gcgccgacac ttggcatact cccggcattt gcgctaccac
     4201 cgcgcaatgg atcgtgtctg cccgaaggga ccattggggt ttttcgccgc ccgcaaggcg
     4261 cgcgacagtt accaccatgg gcatctcaag ctgacgctgc tcgataccgc cgagacgctg
     4321 atcgctgagc gtggcccggc aaacctgtcg ctgtcggaga tggcgcgcat ggctggcgtc
     4381 agttctgcgg cggtctatcg ccattacgcc gacctcaatg cgctgatcgg cgcggtggcg
     4441 catcgcggtt ttgaggactt tgctgcacgg ctgaaggcag gggcggcggt agcgggctcg
     4501 ggcgtggacg gattgcgcgg catgggacgg gcctatctcg attttgcccg cgagcgcccg
     4561 ggtgcctacg cagccatgtt ctcctccacg gtctcctata ccgaccctgg cctggcggcg
     4621 gccggcgaga cggcgttcaa cacgctgatc cagggcgttt ccgccacgtt gtcgaaaagc
     4681 ggcatgccgg ccgaggctgc ctacgggctt gcgctgaaga tttgggcgct ggcccatggc
     4741 gtcgccacgc tggccggatc gggcaggctg acgcctgctg tcggcatcga tccggccact
     4801 ctcctcagcg aggctgtcac tgcactgctt tcggccgaaa gccgctgacg gtttcgtaat
     4861 tcgctgcgcc aacgaagaag ccgttgggtc cttggctgga gtgtaataca ctgaccgccg
     4921 gggcttacag gcggggcatg gacgaccgga gcgacgatca aacgggtttt atggcgtcga
     4981 ccggattgcg gctgcaccgg ggcatcatcg cctttgtcga tgtggtcgaa tcagtgcgcc
     5041 tgatcgccga ggacgggggt gccttcatag cgcgctggct gcaccagcgc agcggtgtcg
     5101 aggccatcct ccagcagcac aacggacggc ttgtccgctc gaccggcgac ggtctcatcg
     5161 gtgaatttgc cgatgcgcgt gccgcgctca aggcggcgtt cgaaacccag gcgcggctgg
     5221 ccagcgccaa tgccggcgcc gaacggccgg tcgtcgtgcg tattggcctg tcgaagggca
     5281 cctattacgc cgacgacaag gacatctacg gcgaggcggt caatcttgcg gcacgcctga
     5341 ccgggcttgg ccagcccgac agcgtcatcg cctcggatgc agtcgccgcc gacctgaccg
     5401 atggcatcga tgcggtgatc gaggacctgg gagcgtgctt tctcaagcat atcgacaaac
     5461 cggtgcgcgc ctatgcgcta cgcccggtcg gaagcatcgc ccgggcaccg gcgctggcac
     5521 gacacccggt ctcgctggtg ccgacgattg ccatcctgcc gctggtccgg ctgcatggcg
     5581 aaggagcgag cgatgccttg ggcgacatca ttgccgacac gctgatcgcc gacctttcgg
     5641 gctcgcccta tctcaacgtc atctcccgca tgtcggcgac gatggtcagc gcaagccggc
     5701 tcgatccgac caaggcggca caagcacttg gggcagatta catcgtctcc ggcagctatg
     5761 ccttgtcctt cggccgcatg cgcaccacgc ttgaactgat ggaacgcaag tccggtctgg
     5821 tgctgatcga ggaacgcttc tcgcttccgg ccgatgcgct ggttgctccg gacttccgcg
     5881 gctttgaaag cttcaccgcg cgcctgttgc atgcggttct gcgctccgag gtgcagcggc
     5941 tcaagcgcca gactttgccc aatctcgaga gtcattcgct gttgctcgga gcggtcggga
     6001 tgatgcaccg gccgcaggaa gacgatttct tctttgccgc gaacgtgctg caaaccctga
     6061 tggatcggca tccgcgcgag ggactgcccg cggcctggct tgcctgctgg tatacgatca
     6121 aggtgcagaa gggcttttcg accgatcccg aaaaagacgg actgcaagcc cgcgaactgg
     6181 cgcgccgcgc gcttgatctt gaagccgaca cgccgctggc gctggcggtg gcggggctga
     6241 ttgaaaccaa tttcggccgc gatttcgagg ccgcctcgca actgcttgaa tgcgctgcca
     6301 acctcaaccc gaacgacaca tttgcgttgc tgcacaaggc tgcgctgcta accttcactg
     6361 accgtggcga agaatcctat cagataagcc gcaaggcccg tctgctttcg ccggttgacc
     6421 cgcaccgcta ctattttgag gccatcgcgg cctctgctgc gttttcggct ggaaaatacc
     6481 cggaggccat cacccatgcg gatgaggcca tccgcgccaa tcggctttat ccttccccgt
     6541 ctcgtgtccg cacgtcggcg ctgtttcatc tggggcagca cgacgaggcg cgtgcagcgg
     6601 cgaaggcatt gctccgggtc gatccggaat tgaccgtcca tcgctggctg agacaatcgc
     6661 cggcggctga ctatgatgtc ggccggcggc ttgctgaggg cttgaagggg gccggcatac
     6721 cggactcctg acatcgggag ctattctgac catgacattc atgaacggat tgaacggcct
     6781 caacggcctc aatggattga acgggctcaa cgggctcaat ggtctcaacg gcctcaacgg
     6841 cctcaatggc ctgaatggtc tcaacggcct caacggcctc aatggcctca acgggctcaa
     6901 cggtctttcg ggctcgctga gcggtggacc gggctcaatc ctcaccacgc acgatgccat
     6961 tgccgggctg tatcaacccc cggctcatgc cccgactgca attccggcag cggcacgcct
     7021 tgatcttggc cgctggtcgg cggatgtgcg ggcccgaatt ctcgatgcgg acctgttccc
     7081 gcgtcttgct ttcggaaaga agccgggggc gcccgatcag tttatggtgc tggccgggca
     7141 gcaaacctgg agcacgaccg gcgccgcgcc gattctggtg cagcaatacg atcctctgtt
     7201 caccatcaac ccgccgacca tcgccagcct cgttagccag caattgccgc tggtggccaa
     7261 tgccgcggtg ctgcgcggcg atcgcctgca tgagatcatt gaccaaatcg ttctgccgat
     7321 cggcttttat ggcgccatcg tgccgctgtc gcccgaacgc aagcgttgga cgctggaact
     7381 gatgatggtg gcattccgtc tgtcggtttt tgtcgagatg gccttcaagc acgctttctc
     7441 gctgccgcgt ccgaacgaga tgtcaccgca gatcaacccg gtgatcccca ccccgctgca
     7501 tgcaacgctg cccagtggcc atgccaccga gaatttcgcc atggcgacga ttctgtggga
     7561 gctgctgcgg cgctctccgg cgcccgagcg cgatcccgcc tatcgtgacc ggctgttcaa
     7621 acttgccgac cgcatcgcct acaaccggac cgtggcgggt gtgcattttc cggtcgacaa
     7681 cgcggctggc gccgcacttg gcacaacgct gggcctgttc ctggttgacc agtgtctcga
     7741 tggcgccggc ggcggctgcc acgaatgggc attcgacgcc gccgtgttct gcaatccggc
     7801 gcagcatgat ggcttcttcg attatgccga cctcgtcggc cagatgttgc ctggcggggg
     7861 cggcgcagca gcaacgcccg tgcccggcct gagccagatg aacggcctgg catttccgcg
     7921 ctccgccctg ctcggcgcat tgtggagcaa ggcgctcgac gagtggtcct gacggaattc
     7981 gccctgtcct gaccccatca aggagacacg cgatgctctg gaccaccggc accagtgcct
     8041 atcaggggct gccgctcgca atggatccgc tggccgactg gattcgcccc gagaaccggc
     8101 cagccgatca ccgcattctt gtgctgatcc gcctggcggc cgggataacg gcccaggacc
     8161 ttccggactt ccccggctcc accgaaacag ggccggtgtt tgtcttcaaa ggcaagccgg
     8221 tcacccaccg tcggtggcaa caggcgctgg ccgttccccg gtgttacacc ggcgcacgcg
     8281 gcccttggaa ccgggcgcgc tattgcactg cgctggcaac gcccgatttc ttccaatggc
     8341 tgctggccga accgaaggtt caggcattgt tcgacgaaat cacgctgtgc atcacgccgg
     8401 atgtcagcga gccacctgcg gacgaccgcg aagccggcgt ggccatcgac tattcgctgc
     8461 cggaagcgaa ggtcgtcacc ggtgtgattg actatggcct gccgctggcc atcccccttt
     8521 acagcggcat tgcgcccgat ggggccaccg tcgcgccgcc gcggctgctg tctgtctggg
     8581 tgcaggatgg cagcccggtg cgtgccagcg cgcataatgg cctcagccgc ccgaccgaat
     8641 tccgctatgg tgccgaactg tcggctaccg ccatccgccg catcatgcga cgggcgggag
     8701 gcgatgagga ccgcttctat cgcatggccg ctctgttcgt tccggcccgc ctcggcacct
     8761 actggaccca gcacacgacg acgcatggcg cccatgttct gggcaacgtt gtcgatccgg
     8821 cggccgacgc cggccataac cctgtcatcg cggtgcatct gccgagcgtg gcggtggagg
     8881 accgcaccgg cgcctcgctt tccggacatg cggtggatgc catgcggttc atcatcgccc
     8941 gcaccgaaga tctctccgat ccggaggatg gccggccgat gccgccgctg gtgatcaacc
     9001 tgagctacgg cttccacgcc ggccctcacg atggcaatgc cccgattgaa aaggcgatga
     9061 ccgagctgca cgccctgtgg agcggtgtct accctgctgc accgttcgtg atcgtcgtgc
     9121 cggcaggcaa tgcctatcag tcgagcacgc atgcctgctg gaccgcgtcc atgctggcca
     9181 gcggcgatgc atccgtgacc tgggaaatca tgcctgacga tgccagttgc aacatcctgc
     9241 agatctggct gccggaaggg ctggccggag gcgcgacagc ccgcctgacc tcgccgcagg
     9301 ggcagaccgt gcagctgt
//
LOCUS       DAOMXG010000094         9140 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_4005_length_9140_cov_8.660099, whole genome shotgun sequence.
ACCESSION   DAOMXG010000094 DAOMXG010000000
VERSION     DAOMXG010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 9140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9140
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4005_length_9140_cov_8.660099"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="PLQ11_07600"
     CDS             <1..340
                     /locus_tag="PLQ11_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24802.1"
                     /translation="PSRAPVPGARTQSPAGETTGAVSSRGDPTAGTALDPLNNRSFDL
                     NSSQTVPRGPLPGGQPANSSQRQGFAPVPPSADMQRRGGGDASQGTRIDPLNNRSFDL
                     NSSKSVPALR"
     gene            413..1297
                     /locus_tag="PLQ11_07605"
     CDS             413..1297
                     /locus_tag="PLQ11_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HRK24803.1"
                     /translation="MALASMTGFARASGVAGAYALAFEMRSVNGRGLDMRFRLPAPFD
                     GLEQDLRTRAQKRLARGTVQIGLTIRRESPAGTVRINEQTFLDVAAQAEKLAWMANLA
                     AATVGDLLRVPGILESGPVEPEALDEAARAATFACFDAALEGLIAARHSEGAALKDVV
                     LQQIDAMQRGIAAAEAADAARPAAIKARLRTQIAALMDSADGLDPQRLHQEAALIAAR
                     VDIREEIDRFRAHVDAARALVNAAEPVGRKLDFLTQEFVREANTLCSKANDIALTRVG
                     LDLKALVEQFREQIQNIE"
     gene            1301..1981
                     /gene="gmk"
                     /locus_tag="PLQ11_07610"
     CDS             1301..1981
                     /gene="gmk"
                     /locus_tag="PLQ11_07610"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HRK24804.1"
                     /translation="MANATSPPASRRGVMLVIASPSGAGKSTLSRLLLQTAKDISMSV
                     SVTTRKRRPSEVDGVHYHFRSVEEFGLMRERGELLEWAEVHGNFYATPRAPVEKALAE
                     GRDVLFDVDVQGTLQLYAAMRDDMATVFILPPSIRELHLRLQRRAEDDTATINRRLRT
                     ALNEIRHWQDYDYVLINDDLDRCFAELQAILNAERLKRSRRAGLAELVSELDRDLESL
                     LEAQARAL"
     gene            complement(1987..2832)
                     /gene="rsmA"
                     /locus_tag="PLQ11_07615"
     CDS             complement(1987..2832)
                     /gene="rsmA"
                     /locus_tag="PLQ11_07615"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HRK24805.1"
                     /translation="MAQIDDLPPLRAVIAAHGLEARKALGQNFLLDLNLTARIARAAG
                     PLAGRTVLEIGPGPGGLTRALLAAGAERVVAIERDARCLPALAEIAARYPGRLTVIEG
                     DALTIDPRPWLNGSPVRIVANLPYNVGTALLVGWLAAPQWPPFWESLTLMFQREVAER
                     IVAGPERPADYGRLGVLAGWRSHARILFDVPAAAFTPPPKVTSSVVELLPRPSPLPCA
                     LTALEAVTAAAFGQRRKMLRQSLKGLGDAPALLATAGIDETRRPETLTIAEFVALAQA
                     LSARP"
     gene            complement(2846..3763)
                     /locus_tag="PLQ11_07620"
     CDS             complement(2846..3763)
                     /locus_tag="PLQ11_07620"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HRK24806.1"
                     /translation="MNNALRAARMTVALLATALLLAVAGPARAQSVAAFVNGEPITTY
                     DLEQRMKINGAMGRRGLSRQQTLQELVDDKLKIIEARRIGYRLSDDNVEEQVTRIARQ
                     NRQSTLEFMQNLAKVGIDSNAYRAKLRADYSWELATSHKFRTSGAASGSEVDSIFQSK
                     VRDGGAKVTDYVLQSVVFVVGTGVSPATREREANGARSRFDGCENGIEPLRQMRDVAI
                     KSPVKRSSNTLSPQLAAVLAKTPSGKMTPPFRSDQGIELIAVCEKTERVDTAAARSQA
                     EEEVAAKKRVSETDTYLKSLRSKAVIQYR"
     gene            complement(3774..6350)
                     /locus_tag="PLQ11_07625"
     CDS             complement(3774..6350)
                     /locus_tag="PLQ11_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HRK24807.1"
                     /translation="MPVASILLLRTRLLAAASALVLLVAATQGVLAQDKKDKKDKMLV
                     EANELVYDKDNDTVTARGNAQIYYQGRSLEADKVTYNRKTKRVFAEGNARMTDTNGTR
                     YYGDRFELTDDFKDGFIDSLRSEAPDKQRFAAIRGERTDGKTSVFDRGTYTACEPCKD
                     DPAKPPLWQVRAARIIHKSEEQTIYYEQATLEFWGVPIAYMPFFSGPDPSVSRKSGFM
                     APIIVSKKSLGQGVSIPYFWAIAPNMDLTLTPTFLSRQGVLGQAEFRHRLNNGMYSIR
                     AAGIFQRDPSAFLPNPLGGGSSTFRGSIETVGRFHINERWRWGWSLAASTDKFFYNNY
                     RIRTTTLSNTYLKESISTLFLNGQGDRSWFDLRGYYFRPLSQTEWQKQQALVHPVFDY
                     DKRFSNPFFGGEFQLTANLTSLSREAANFSGLPGPGTVGNYVSPALFSGIAFGKAGQA
                     YGYSYGEGCVQYIPGSCLLRGVGGDYTRASLAVSWRRTLIDPIGQVWTPFASLRGDLG
                     FMGLNNSKYVYAPNDLNVYGNDKQRFFLSDNTNIAARMMPMVGVEYRYPFVARHAGFT
                     HQVEPIAQLIVRPNETRIGRFPNEDAHSLVFDDTLLFARDKFSGFDRVEGGVRANVGV
                     QYTLTSDSGAYANMLFGQSYHLSGRNSFAQTDMINTGLNSGLESRQSDFVSRITVAPG
                     SAFNVTARGRFDEKTFALKRMEVGATATLGAVSVNAGYARLAPQPLLGYNLRREGMIL
                     GVAVQLPSSWYVSSGVSFDMDRYLSDRSYHLSNPTLYPTYRNTPFRVASLSLGLGYRD
                     ECTDFSLTYSRSRSDTVLDGTVKTGSVFLFRLELKHLGQAQYRLSSGLADTSGQADGL
                     GR"
     gene            complement(6350..7432)
                     /gene="lptG"
                     /locus_tag="PLQ11_07630"
     CDS             complement(6350..7432)
                     /gene="lptG"
                     /locus_tag="PLQ11_07630"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003604387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HRK24808.1"
                     /translation="MISTLGRYFGMRFLSAIAGVFLTVFVLIFTIDLVELMRRAGEAE
                     GASTMRLARLALFRTPAVAEQVLPFAVLFAALATFLNLSRRLELVVARAAGVSAWQFS
                     LPIVALAIIIGVLATTAYNPLAMHLKDEANRIETQLFQRKGAGGSERGVWFRQRSVDG
                     DAILRAASLDQSAFILHDVTAFVFDTNGDFLERVEAPQAQLVGNHWRFQDARVLSVGI
                     EPQQVDRYLLATYLSAAEIRSAVGQAQAVSFWSLPDVVRTLDLAGLDATRYRLKYQSL
                     LARPALLTAMVLIAASVSLRFLRSGGFAGMVLSGVAAGFVLYVATRIVEDLGTAGLVS
                     TVFAAWAAPVIGGMMGVLTLLHREDG"
     gene            complement(7438..8655)
                     /gene="lptF"
                     /locus_tag="PLQ11_07635"
     CDS             complement(7438..8655)
                     /gene="lptF"
                     /locus_tag="PLQ11_07635"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HRK24809.1"
                     /translation="MRILERYIFRTAMSAFLLCLIALTGVIWITSALRELDLVTGKGQ
                     SILMFLYVTLLSVPALVMIIAPLALFIAILYTLNKLNGDSELIVMNAAGVPPWRILRP
                     FLLLTVLVSLLVAAITVKIMPDSFRELRHLVTKIRADVITQFIQEGKFVSLEQGITFH
                     YRDKGPDNTLRGIMFQDRRNPSLVATYLAERGRMVEVDDRTYFILEKGSLQRQDQARA
                     EAALVYFERYALDLDQLGESKGSIGYKPRERTTAELFQPDSDDPYVREQIGRFRSELH
                     ERFSQPLYAYATLAIGFAALGSARTTRQGRGAAMAAAGMAMLWLRIAGFWASSLAVRS
                     ASAVMLMYLVPILVTVIAGTVAWWGQDRPALLARWIAQARSLWHRSGLSDRLHLPDLR
                     ARLAALVIRGARP"
     gene            8893..>9140
                     /locus_tag="PLQ11_07640"
     CDS             8893..>9140
                     /locus_tag="PLQ11_07640"
                     /inference="COORDINATES: protein motif:HMM:NF014808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HRK24810.1"
                     /translation="MSRGPKITFSSSDSALHPVAVVFAGEGLALADGAARMGPDFPAL
                     LKAAAEGESFTGKAGSRLDLVAPAKVPAARVIVCGIGG"
BASE COUNT         1757 a         2886 c         2974 g         1523 t
ORIGIN      
        1 gccctcccgc gccccggttc cgggcgcccg cacccagtca ccggcaggcg agaccaccgg
       61 cgcggtgagc agtcgcggcg atccgaccgc aggcacagcg ctcgatccgc tcaacaaccg
      121 gtccttcgac ctcaattcgt cccagacggt gccgcgcggg ccgttaccgg gcggccagcc
      181 tgccaactcg tcgcagcgac agggctttgc ccctgtgccg ccctcggcgg acatgcagcg
      241 gcgcggtggc ggcgatgcca gccagggcac gcgcatcgac ccgctcaaca accgctcctt
      301 cgatctcaat tcatcgaagt cggtgccggc gctgcgctga cctattgccg ggcctttccg
      361 accggcacga gaatcagaaa cactggcttt catctgaact gggagcgatc gcatggcact
      421 cgccagcatg accggatttg cgcgggccag cggcgttgcc ggcgcctatg cgctggcgtt
      481 tgaaatgcgc agcgtcaatg gccgcgggct ggatatgcgc tttcggctgc cggctccgtt
      541 tgatgggctg gagcaggatt tgcgcacgcg ggcgcagaag cggctggcac gcggcaccgt
      601 gcagatcggg ctgacaatca ggcgggagag cccagccggg accgtgcgga tcaacgaaca
      661 gacgtttctc gatgttgccg cgcaggcgga aaagctggcc tggatggcca atctcgccgc
      721 cgccacggtg ggggatctgc tgcgggttcc gggtatcctc gaatccggcc cggtggaacc
      781 cgaggcgctc gatgaagcgg cgcgcgcggc cacattcgcc tgtttcgatg cggcgctcga
      841 agggttgatt gccgcccggc acagcgaagg ggcggcactg aaggacgtgg tcctgcagca
      901 gatcgacgcc atgcagcggg gcattgccgc agccgaggcg gcggatgcag cccgcccggc
      961 agcgatcaag gcccggctca ggacccagat tgccgcgctg atggactcgg ccgacgggct
     1021 cgatccgcag cggctgcatc aggaggcggc gctgatcgcc gcccgcgtcg atatccggga
     1081 agagatcgac cggtttcgcg ctcatgtcga tgcggcgcgt gcgctggtca atgccgccga
     1141 accggtcggg cgcaagcttg atttcctaac ccaggaattc gtccgggaag ccaatacgct
     1201 gtgttccaag gccaatgaca tcgcgctgac ccgggtcgga cttgacctga aggcgctggt
     1261 cgagcaattc cgcgagcaga tccagaacat cgagtgagcc atggccaacg ccacgtcccc
     1321 ccccgcatcc cgtcgcggcg tgatgctcgt gatcgcctct ccctccggcg ccggcaagtc
     1381 gacgctcagc cggctgctgc tgcaaaccgc caaggacatt tcgatgtcgg tctcggtgac
     1441 gacgcgcaag cggcgcccga gcgaggtgga cggcgtgcac taccatttcc gttcggtcga
     1501 ggagttcggc ctgatgcgcg agcgtggcga gttgctcgaa tgggccgagg tgcatggcaa
     1561 tttctacgca acgcccagag cgccggtgga aaaggccctg gccgaggggc gcgatgtcct
     1621 gttcgacgtt gacgtgcagg gcacgctgca gctttacgcg gcgatgcgcg acgacatggc
     1681 gaccgtgttc atcctgccgc cgtcgatccg cgagttgcat ctgcgcctgc aacgccgcgc
     1741 cgaagacgat accgccacca tcaaccggcg gctcaggacg gcgctgaacg aaatccgcca
     1801 ctggcaggat tacgactacg tgctgatcaa tgatgatctt gaccgctgct ttgccgaatt
     1861 gcaggccatt ctcaatgccg agcggctgaa gcgcagccgg cgtgccggtc tggccgaact
     1921 ggtcagcgaa ctcgatcgcg acctggaaag tttgctggag gcgcaggcca gggcgctgtg
     1981 aggctgtcag ggccgcgccg aaagggcctg tgccagggcg acgaattcgg caattgtcag
     2041 cgtctcgggg cgccgtgtct cgtcaattcc ggcagtggcc aacagagccg gtgcatcgcc
     2101 cagccccttg aggctctgcc gcagcatctt gcgccgctgt ccgaaggctg ccgccgttac
     2161 ggcttccagc gccgtcaagg cgcagggcag gggcgaagga cggggaagca gctcgacaac
     2221 cgacgaggtg accttgggcg gcggggtgaa cgccgccgcc ggcacgtcga acaggatgcg
     2281 ggcatgactg cgccagccgg ccaggacgcc gaggcgtccg taatcggccg ggcgctctgg
     2341 cccggcgacg atgcgctctg cgacctcgcg ctggaacatc agggtgaggc tctcccagaa
     2401 cggcggccat tgcggcgccg ccagccatcc gaccagcaaa gcggtgccga cattgtaggg
     2461 caggttggcg acgatgcgca cgggggagcc gttgagccag gggcgggggt cgatcgtcag
     2521 cgcgtcgccc tcgatcaccg tgaggcggcc aggataacgt gcggcgatct ccgccagcgc
     2581 cggcaggcaa cgggcgtccc gttcgatcgc aaccacccgc tctgctccgg cggcgagcag
     2641 cgcgcgcgtc aacccacccg ggccggggcc gatttcaagc acggtccggc cggcaagcgg
     2701 gccggcggcg cgggcaatcc ttgcggtcag attgagatcg agcaggaagt tctggccgag
     2761 cgccttgcgc gcctccagac catgagcggc aatgaccgcg cgcagcggcg ggaggtcatc
     2821 gatctgcgcc atggctgccg accgatcagc gatactggat gacggccttg ctgcgcagcg
     2881 acttcagata ggtgtccgtt tcggagaccc gcttcttggc cgccacctct tcttcggcct
     2941 gggagcgggc ggccgcagtg tcgacccgct cggtcttttc acagacggcg atgagttcaa
     3001 tgccctggtc tgaacggaat ggcggtgtca tcttgcccga aggcgtcttg gccagcaccg
     3061 ctgcgagctg cggcgacagg gtattggacg aacgcttgac cggcgacttg atcgcgacat
     3121 cgcgcatctg acgcagcggc tcgatcccgt tctcgcagcc atcaaaccgg ctgcgggcgc
     3181 cattggcctc acgttcgcgc gtggcggggc tgacgccggt gccaaccacg aacacgaccg
     3241 actgcaacac gtaatcggtg accttggcac cgccgtcgcg caccttcgac tggaagatcg
     3301 aatcgacctc actgccggag gcagcgccag aggtgcgaaa cttgtgactg gtggccagtt
     3361 cccacgaata atcggcgcgc agcttggccc ggtaggcatt ggaatcgatg ccgaccttgg
     3421 ccagattctg catgaattcg agggtgctct gccggttctg gcgggcaatg cgcgtgacct
     3481 gctcttcgac attgtcgtca gacaatcgat agccgatacg ccgcgcctcg atgatcttca
     3541 gcttgtcgtc aaccagttcc tgcagcgtct gctggcgtga cagcccgcgc cgccccatcg
     3601 caccgttgat cttcatccgt tgctcaagat cataggtggt gatcggctcg ccattgacga
     3661 aagcggccac tgactgggcg cgagcagggc cagcgacagc caggagcagc gccgtggcga
     3721 gaagggcaac agtcatgcgg gcggctctca aggcgttgtt catggggctg tccttacctg
     3781 cccaggccat cggcctgccc ggatgtatcc gccaggccgg atgacaggcg atattgtgcc
     3841 tggccaaggt gcttgagttc cagccggaac aggaaaacag acccggtctt caccgttcca
     3901 tcaagcaccg tatcgctgcg cgacctggaa taggtcagcg agaaatccgt gcattcatcg
     3961 cgatagccga ggccaagcga cagcgatgcg acgcggaacg gcgtgttccg gtaggtcgga
     4021 tacagcgtcg ggttggagag gtgatacgaa cggtcagaaa ggtaacggtc catatcgaaa
     4081 ctgacgccgc tgctcacata ccagctggac ggcagctgca ctgcaacgcc aaggatcatg
     4141 ccttcgcggc gcagattgta tcccagcaga ggttgcggtg ccaggcgggc atagccggca
     4201 ttcaccgaca cagccccgag ggtggccgtc gccccaactt ccatgcgctt gagcgcaaag
     4261 gtcttttcat cgaagcggcc gcgcgccgtg acgttgaacg ccgaacctgg ggcaaccgtg
     4321 atgcgcgaga cgaaatcgga ctgccgtgac tcaagcccgc tgttcagccc ggtgtttatc
     4381 atgtccgtct gggcgaagga attgcgcccg gacaggtgat aggactggcc gaacagcatg
     4441 ttggcatagg cgccggaatc agaggtcagc gtatactgaa cgccgacatt ggcgcgcacc
     4501 ccgccttcca cccggtcaaa accggagaac ttgtcgcgcg cgaacagcag cgtgtcgtcg
     4561 aacaccaggc tatgcgcatc ctcgtttggg aagcggccga tgcgggtctc gttggggcgg
     4621 acgatcagct gggcaatcgg ctcgacctga tgagtgaacc cggcatggcg cgcgacgaac
     4681 ggatagcgat attcgacgcc gaccatcggc atcatccgcg cggcgatgtt ggtattgtca
     4741 gacaggaaga agcgctgctt gtcattgccg taaacgttga gatcgttcgg cgcgtagacg
     4801 tatttgctgt tgttgaggcc catgaagccg aggtcgccgc ggagcgaggc aaacggcgtc
     4861 caaacctggc cgatcgggtc gatcagcgtc cgccgccagc ttaccgccag cgaagcccgg
     4921 gtgtaatccc cgccaacgcc gcgcaacagg cagctgccgg gaatatactg cacgcagccc
     4981 tcgccgtagc tgtagccgta ggcctggccg gctttgccga aagcgatacc gctgaacagc
     5041 gctggggaaa cataattgcc gaccgtcccc ggtcccggca ggccggagaa gttggcggcc
     5101 tcgcgcgaca ggctggtcag attggcggtc aactggaatt cgccaccgaa gaacggattc
     5161 gaaaaccgct tgtcatagtc aaacaccggg tgaaccagcg cctgctgctt ctgccattcg
     5221 gtctgtgaaa ggggacggaa ataatagccg cgcaggtcga accagctgcg atccccctgt
     5281 ccattaagga acagcgtcga gatcgactcc ttcaggtagg tgttcgacag cgtcgtcgta
     5341 cggatacggt agttgttgta gaagaacttg tctgtcgagg cggcaaggct ccagccccag
     5401 cgccagcgct cgttgatatg gaagcgaccg actgtctcga tgctgccgcg gaatgtcgaa
     5461 ctgccgccac ccagaggatt gggcaggaac gcgctcgggt cacgctggaa aatgccggcg
     5521 gcacggatcg aatacatgcc gttgttcagc cgatggcgga actcggcctg cccgagcacg
     5581 ccctgccgcg acaggaaggt cggggtcagc gtcaggtcca tgttgggcgc aatcgcccag
     5641 aaatagggaa tcgagacacc ctggccgagc gacttcttgg acacgatgat cggcgccatg
     5701 aagccggact tgcggctcac actcgggtcg ggacccgaga agaacggcat gtaggcaatc
     5761 ggcacgcccc agaattcgag cgtcgcctgc tcgtagtaga tcgtctgctc ctcgctcttg
     5821 tggatgatgc gggcggcccg cacctgccaa agcggaggct tggccgggtc atccttgcac
     5881 ggttcgcagg cggtataggt gccacgatca aagaccgagg tcttgccatc ggtccgctcg
     5941 ccgcggatag ccgcgaaacg ctgcttgtcc ggcgcctccg agcgcaggga atcgatgaac
     6001 ccgtccttga aatcatccgt cagttcaaaa cggtcgccgt aatagcgggt gccgttcgta
     6061 tcggtcatgc gcgcattgcc ctcggcaaag acgcgcttgg tcttgcgatt gtaggtaacc
     6121 ttgtcggctt cgagtgaccg gccctgataa tagatctgcg cgttgccgcg ggcggtgacg
     6181 gtatcgttat ccttgtcgta gacgagctca ttcgcctcga ccagcatctt gtccttcttg
     6241 tccttcttgt cctgcgccag aacgccctgc gttgccgcca caagcagcac cagcgccgaa
     6301 gcagcagcca gcaatcgggt tcggagcaga aggatggagg cgaccggcat cagccatcct
     6361 cccggtgcag cagcgtgaga acgcccatca tgccgccgat gaccggtgcc gcccaggcag
     6421 caaaaaccgt cgacaccaag ccggcggtgc ccagatcctc gacaatgcgc gtcgctacat
     6481 acaagacaaa accagccgct acgccactca acaccattcc ggcgaaacca cctgatcgca
     6541 aaaaccttaa ggaaacggat gcagcgatca gaaccatggc agtgagcaac gccggacggg
     6601 ccaagagaga ttgatatttc agccgatacc gcgtcgcgtc gagccctgca agatcaagtg
     6661 tgcgaacaac atccggtagc gaccagaacg acacagcctg cgcctgcccg acagcactgc
     6721 ggatttcggc cgccgaaagg taggtggcca gaagataccg atcgacctgc tgcggttcga
     6781 tccccaccga taacaccctc gcatcctgaa aacgccagtg attgccgaca agctgggcct
     6841 gcggtgcctc gacccgctcc aggaaatcac cgttggtgtc gaatacgaag gccgtcacat
     6901 catgcaggat gaacgcgctc tggtcgagcg aggcggcccg caggatcgca tccccgtcaa
     6961 ccgaacgctg gcggaaccag acgccgcgct cgctgccgcc ggcgcccttg cgctgaaaca
     7021 gctgcgtttc gattcgattg gcctcatcct tcagatgcat cgccagcgga ttgtaggcgg
     7081 ttgtcgccaa cacaccaatg atgattgcca gggcgacaat cggcagggaa aactgccagg
     7141 ccgagacgcc ggcggcgcgg gcgacaacca gctccagccg gcgcgacagg ttcagaaaag
     7201 tcgccagcgc ggcaaacagc acggcaaacg gcaagacctg ctcggccacc gccggggtgc
     7261 gaaacaaggc cagccgcgcc agccgcatgg tgctggcgcc ctcggcctcg ccggcccggc
     7321 gcatcagttc caccaggtcg atggtgaaga tcagcacaaa aaccgtcagg aacacgccgg
     7381 cgatggcgct gagaaagcgc atcccgaaat agcggcccag ggttgagatc atggtgttca
     7441 tgggcgggca ccccgtatga ccagcgccgc caggcgtgcc ctgaggtccg gcaggtgcag
     7501 ccggtcggac agcccgctgc gatgccacag ggatcgtgcc tgggcgatcc accgcgccag
     7561 cagcgccggt cggtcctgcc cccaccaggc cacggtcccg gcgatgacgg tcaccaggat
     7621 cgggaccaga tacatcagca tgaccgccga tgcgctgcgt acggccaggg atgaggccca
     7681 gaaaccggcg atcctgagcc acagcatcgc catcccggcc gccgccattg ctgcgccgcg
     7741 tccctggcgg gtggtgcgcg ccgagccgag cgcagcgaat ccgatcgcca gagtggcgta
     7801 ggcatagagc ggctgggaaa accgctcgtg caactcggag cggaaccggc cgatctgctc
     7861 gcgcacgtat ggatcatcgg agtccggctg gaacaactcg gcggttgtgc gctcgcgcgg
     7921 cttgtaaccg atcgatccct tgctctcgcc aagctggtcc aggtcgagcg cataacgctc
     7981 gaagtagacc agcgcggctt ccgcgcgcgc ctggtcctgc ctttgcaggc tgcctttttc
     8041 gaggatgaaa taggtccggt catccacctc aaccatgcga ccgcgttccg ccagataggt
     8101 ggccacgagg ctgggattgc gccggtcctg gaacatgatg ccgcgcaagg tgttgtccgg
     8161 ccccttatcg cggtagtgga aagtaattcc ctgttcgagc gagacgaatt tgccttcctg
     8221 gatgaactgg gtgatcacgt cggcgcggat cttggtcacc agatggcgca gttcacgaaa
     8281 cgagtccggc atgatcttga cggtaatggc cgccaccagc agcgaaacca gcaccgtgag
     8341 cagaaggaac ggccggagga tgcgccacgg cggcacgccg gccgcgttca tgacgatcaa
     8401 ctcgctgtcg ccgttgagct tgttcagcgt gtagagaatc gcgatgaaca gcgccagcgg
     8461 ggcgatgatc atcaccagcg ccggaaccga cagcagggtg acatagagga acatcaggat
     8521 cgattgtccc ttgccggtca ccagatcgag ttcgcgcaaa gcggaggtga tccagataac
     8581 gccggtcagc gcgatcaggc agagcaggaa ggcgctcatc gccgtccgga atatgtagcg
     8641 ctccagtatc cgcatgcagt ccggttcttg atgtctccca aggggccaac gacagatcca
     8701 aacgcccctt tccggcattg ataaggcagg cggcggcgat tgtcctatcc cgctcgcccg
     8761 taagcggtgc aatgccgcga ggctgtggca aaaaaacaag catgacaagt gcttgggcgc
     8821 cgtctttgct acactggcga cgattcgacc gtggcaatcc gcgccgcggt cagggcaggg
     8881 aaggatgagg atatgtcacg cgggccaaag atcacgtttt catccagcga ttcggcgctg
     8941 catccggtcg ccgtggtgtt cgccggcgag ggtctggcgc tagcggatgg agcggcgcgg
     9001 atggggccgg attttccggc actgcttaag gccgccgccg agggggagag tttcaccggc
     9061 aaggccggct cgcggctcga ccttgtcgcg cccgccaagg tgccggcggc ccgggtcatc
     9121 gtctgcggca tcggggggga
//
LOCUS       DAOMXG010000095         9129 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_4015_length_9129_cov_8.814415, whole genome shotgun sequence.
ACCESSION   DAOMXG010000095 DAOMXG010000000
VERSION     DAOMXG010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 9129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9129
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4015_length_9129_cov_8.814415"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..527)
                     /locus_tag="PLQ11_07645"
     CDS             complement(117..527)
                     /locus_tag="PLQ11_07645"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRK24811.1"
                     /translation="MSRRQWLVAAFTVLPGAAWAQPDLPSASVAEAFAAAQDGRMVIV
                     DIRRPEEWAETGVARGAVKLDMTAADFPQRLMALRQASGNREIGIICRTANRTDYVQK
                     ALARHNIRLVNIRGGMAGNPGNPGWIASGLPIEK"
     gene            complement(547..1248)
                     /locus_tag="PLQ11_07650"
     CDS             complement(547..1248)
                     /locus_tag="PLQ11_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018963942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HRK24812.1"
                     /translation="MPVDNSGRTVRAWDLPTRLFHWALVVLIILAPITNKMGSATFYL
                     HKVNGYLILSLVLYRILWGFVGGTTARFRTFVRPFAAFGYLGDLVRNRSRHFLGHNPL
                     GGMMVLALLAAAGGQALFGLMTLDDIPATIEGPFASKVAKSTAEWVSVWHRRGFKIIL
                     ALVALHVLANLFYTFVKKDNLIRPMLTGVKPAGAYEDVAENQPGSLARAALCLALAIV
                     VVFGSVWVFGSAPFR"
     gene            complement(1341..1778)
                     /locus_tag="PLQ11_07655"
     CDS             complement(1341..1778)
                     /locus_tag="PLQ11_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRK24813.1"
                     /translation="MIRTLVVAAALAIGATAVVAQGDVIAQRKDAMKGVGDATKPIGA
                     MLKGEMKFELAAAQNALKVYQMVSKDYDKMFPDNSKTGGETTAAPKIWEDRAGFKKLL
                     DKLNADAGAAAGAIKDEASFKTEMGKVLGNCKACHDDYRVKKS"
     gene            complement(1939..4233)
                     /locus_tag="PLQ11_07660"
     CDS             complement(1939..4233)
                     /locus_tag="PLQ11_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009737004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HRK24814.1"
                     /translation="MRPMKFGMGQAVRRKEDQRFITGAGHYATDYRPEGALTAVLVRS
                     PHAHATFTIANIDDVRAMKGVRLAWSAADVADLGPLPCQALQRNLNGKRMVATDYAVL
                     ARGEVRHVGDAVAFIVASTLEQAKDAAEALEVHYEELPAAVGIAAAMLPGAPQVWSDV
                     EGNVALDTAIGDAKATDAAFAKADRVVSVTLVNNRLVANYMEPRVCTAEYDAKSRQYL
                     LTLGSQGSHGLRDTLARILKEKPEKIRVVTPDVGGGFGNKAFMYREYPLLAKAARVLK
                     KPVCWVQERTEHFLSCAQGRDNLVTAEMALSKSGRFLAMRVDLKADFGAYLSQYAPYI
                     PYLGGTMTTGCYDIPQAYFRVRGIYTHTVPVDAYRGAGRPEAAYLIERLVDRIALETG
                     TTPDRVRARNFIKPSQFPYRTPMDRVYDSGEFEGHMRRAMDIADWKGFARRAAAARKR
                     GLHRGIGMACYIEACGGGSPEPAYIGLDADGGVTVKIGTQSNGQGHETAYAQLASQHL
                     DLAMDRIRVLQGDTDQTPTGGGTGGSRSIPVGGAAVAAAAAKLAENIKLIAASEFEAS
                     AGDLELAEGRVRVAGTDRSITFAEIARSKKATVELLNTTGSFSPPEATYPNGTHLVEV
                     EVEGDTGAVRIVNYVIVDDFGRTLNPLLLEGQVQGGTAQGIGQALSERTVFAEDGQLL
                     TASFMDYGVPRAADLPSFTFETRNVPCTTNVLGVKGAGEAGSIGSCPAVMNALVDALH
                     RANGTRHIDMPATPEVVFKALTSA"
     gene            complement(4264..4584)
                     /locus_tag="PLQ11_07665"
     CDS             complement(4264..4584)
                     /locus_tag="PLQ11_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019904953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRK24815.1"
                     /translation="MNAIREVSRSEVKAGMADGSLVLVDVREDHEFAAGHVPGSVSAP
                     LSRLSAADLAPFKDKRIVFSCAAGVRSQHAIAVVQAEGLSLNEHYRGGFRDWVMAGEA
                     IRAG"
     gene            4706..5467
                     /locus_tag="PLQ11_07670"
     CDS             4706..5467
                     /locus_tag="PLQ11_07670"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRK24816.1"
                     /translation="MLLEGSQLWFGLVGALLIGLGKGGLGSGFGMLVVPVLSLTLDPR
                     QAAALTLPILITSDFFAVYAYRGKAHTGHMRVLLTGAVIGIGLGALTFHLVSEPMLRL
                     MMGLMALIYVVSRFLGLARPSDVPLPPDWGQGVTLGTISGFTSFTAHAGGPPVQVYML
                     PKGLDKQAFQATNVLLFTLINLIKVPPYLALGQFTGPVMLTSLMLMPVAAGGVFLGYR
                     LHRWMPETLFFRIAVTLLFLTGLKLTWDGLGALLF"
     gene            5541..6284
                     /locus_tag="PLQ11_07675"
     CDS             5541..6284
                     /locus_tag="PLQ11_07675"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HRK24817.1"
                     /translation="MRLKGKVAIVTGGAQGFGEGIVRAFLGEGAKVAILDIKTAEAAA
                     LAKELGKDTAAFACDVASLASVREAVAAVVRHFGRLDIVVNNAGWTYSNKPATETTEA
                     EFDKVFAVNVKSIYNFTATATEHLAATQGSFINIGSTAGIRPRPGLVWYNATKGAVHN
                     ATKVLAVELAPRKIRVNAIAPVAGETPLLASFMGTDTPERRAQFLATIPLGRFSQPRD
                     IANAAVFLASAESEFITGVVLEVDGGRCV"
     gene            6299..7828
                     /locus_tag="PLQ11_07680"
     CDS             6299..7828
                     /locus_tag="PLQ11_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ligase PGM1-related protein"
                     /protein_id="HRK24818.1"
                     /translation="MTAAAPGPDMPPTPADRARFAGLQADFARSFARLVTDPNAPRTV
                     VILPSLSLDADVLARISGVHHYEERMLCLLLLLRMPRTEVIYLTSMPIPDTIIDYYLH
                     LLPGIPMQHARRRLTLIACDDASPVALSRKLLARPRLLSRIGESIRHPKDAHLSCFNV
                     TDDERRLALALNLPIYGCDPDLAHWGSKSGSRKVFRAAGLDMPAGAEDLRDQQDIVDA
                     LLDLKRRKPGLRRAVVKLNEGFSGEGNATFRFDGAPEGASLAPWLKGRLPHLAFEASG
                     MTWDLFRSKFSAMGGIVEEWVEGAAKRSPSAQMRVDPGGQPQAISTHDQVLGGPSGQI
                     FLGATFPADAAYRKTVQDAGLAAASVLAEKGVIGRFGVDFLSVPEGGGWSHKAIEINL
                     RKGGTTHPFLMLQFLTDGTYDTESGQFHAAAGQPRCYYATDTLEAKRYRGLTPADLID
                     IAALNGLYFHAGQQEGVTFHLIGALSEFGKLGVVAIGADPARADALYRRTVAALDRET
                     A"
     gene            complement(8122..9054)
                     /locus_tag="PLQ11_07685"
     CDS             complement(8122..9054)
                     /locus_tag="PLQ11_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRK24819.1"
                     /translation="MTRRYPLKRTRLSGPVPMLTAVDDVSFTVQAGRSMGIVGESGSG
                     KSTLARLVMALERPDSGTIAFAGEDLNGLDEADLRARRRHFQMVFQDPFSSLDPRMKI
                     VDAIAEPLEVAEPELTAAERRQRVAAMLDSVALPGEALDRYPHEFSGGQRQRVALARA
                     LITGPKLVIADEPVSALDVSVQAQVLNLMADLREARGLTYLFISHNLAVVEHVADDVL
                     VLYRGRVVEYGETSSVFDNPAHPYTRALVDAVPGIDANEARERVRLRDDGGKASGGCG
                     FAGRCPFAADRCRTEVPPLHDHGGRKVACHLAGS"
BASE COUNT         1633 a         3176 c         2761 g         1559 t
ORIGIN      
        1 catgcgaggg gcagaaacgg cctgagtttc gtacgccccg agccacgcga ggggcagaaa
       61 cggcctgagt ttcgaacgcc ccgagccacg cgaggggcag aaaacagcag aactggctat
      121 ttctcgatcg gcagccccga tgcgatccag cccggattgc cgggattgcc agccatgccg
      181 ccacgaatat tgaccaggcg gatattgtgc ctggccaggg ccttctgaac atagtcggtc
      241 cggttggcgg tgcggcagat gatgccgatc tcccggttcc ccgaggcctg ccggagcgcc
      301 atcagccgct ggggaaagtc tgcagcggtc atgtccagct tgacggcgcc gcgcgccacc
      361 cctgtctccg cccattcctc cggacgacga atgtcaacga tcaccatacg cccgtcctga
      421 gccgccgcga acgcctcggc caccgaggcc gacggcagat ccggctgcgc ccatgcggct
      481 cccggtagca ccgtgaaggc agcgacaagc cactggcgcc gcgacaaagg cgccctgcat
      541 tttccgtcag cggaatggcg cactgccgaa cacccagaca ctgccaaaga cgacgacgat
      601 ggccagcgcc agacacagcg ctgccctggc cagcgagccc ggctggttct cggccacatc
      661 ctcgtaagcc cctgccggtt tcactccggt gagcatcggc cggatcagat tgtccttttt
      721 aacgaatgta tagaacaggt tggccagcac atgcagcgcc accagcgcca ggatgatctt
      781 aaagccccgc cgatgccaga ccgaaaccca ttcggccgtg cttttcgcca ccttcgaggc
      841 aaacggcccc tcgatcgtgg cgggaatgtc atccagcgtc atcaggccga acagcgcctg
      901 cccgcccgct gccgccagca gcgccagcac catcatgccg ccaagcggat tgtgacccag
      961 aaaatgccgg ctgcgattgc gcacgagatc gccaagatag ccgaaggcag cgaagggccg
     1021 cacaaaggtc cgaaaccgcg ccgtcgtgcc cccgacaaag ccccacagga tgcgatacag
     1081 caccagcgac aggatcagat agccgttgac cttgtgcaga tagaaggtcg ccgaccccat
     1141 cttgttggta atcggcgcaa ggatgatcag aacaaccagc gcccagtgaa acagccgggt
     1201 cggcagatcc caggcacgaa ccgtgcggcc cgaattgtca acaggcatcg cgccctctca
     1261 tcggtcaaga caggcaataa aaagcagcgc cttccgcatg cgtcaacatg cggaaggccc
     1321 ggtgagtgcg gcctgcggtc tcagctcttc ttgacgcggt aatcgtcgtg gcaggctttg
     1381 cagttgccaa gaaccttgcc catctcggtc ttgaacgacg cctcatcctt gatggcgccc
     1441 gccgcagcgc cggcatcggc gttcagcttg tcgagcagct tcttgaaccc ggcacggtct
     1501 tcccagatct tcggggcggc tgtggtctcg ccgccggtct tggagttgtc cgggaacatc
     1561 ttgtcataat ccttgctgac catctggtag accttcagcg cattctgcgc cgccgccagc
     1621 tcaaacttca tttcgccctt cagcatggca ccgatcggct tggtggcatc gccgacgccc
     1681 ttcattgcat ccttgcgctg cgcaatcaca tcgccctggg cgacaacggc ggtggcaccg
     1741 atggcgagtg cggcggcgac aacaagtgta cggatcattc ggggtctctc cggtggttgt
     1801 tgaatcagat caaaagctgt ccagtcagcc ttgcgcatac gacacttgcc gaaagatgaa
     1861 taccagacgg ctccctgcaa aggctacctt gcgtccttgc aagctcacaa atgcgtgaac
     1921 gccctcgatg atgacgattc aggcggaggt cagggccttg aacaccacct ccggcgtcgc
     1981 cggcatatcg atatgccggg tcccgttggc gcggtggagc gcgtcaacca gagcattcat
     2041 caccgccggg catgagccga tcgaccctgc ctctccggcg cccttgacgc cgagcacatt
     2101 ggttgtgcac ggcacattgc gcgtctcgaa ggtgaaggat ggcagatcag cggcgcgcgg
     2161 cacgccgtaa tccatgaagc tcgccgtcag caattgcccg tcctcggcaa acaccgtgcg
     2221 ctcggacaaa gcctggccga tgccctgcgc ggtgccgccc tgcacctgcc cttccagcag
     2281 cagtggattg agcgtgcggc cgaaatcatc gacgatcacg taattgacta tgcggacagc
     2341 gccggtatcg ccctccacct cgacttcgac aagatgcgtg ccgttcggat aggtcgcttc
     2401 cggtggcgaa aaactccccg tggtgttgag gagttcgacc gtcgcctttt tcgaacgggc
     2461 aatctcggca aaggtgatcg agcggtcggt gccagccacg cgcacccgcc cctcggcaag
     2521 ttcgagatcg ccggccgagg cttcgaattc gctggccgcg atcagcttga tgttttcggc
     2581 cagtttcgcc gccgccgccg cgaccgccgc cccgccgacc gggatcgagc gtgacccgcc
     2641 ggtgccgcca ccggtcggcg tctggtcggt gtcgccctgc aacacgcgga tcctgtccat
     2701 cgccaggtcg agatgctggc tggcaagctg ggcataggcc gtctcatgcc cctgcccgtt
     2761 cgattgcgtg ccgatcttca ccgtcacgcc gccatcggca tcgagcccga tataggccgg
     2821 ctcgggcgaa ccgccgccac aggcctcgat gtagcaggcc atgccaatgc cgcgatgaag
     2881 cccgcgcttg cgcgccgccg ccgcgcgtct ggcaaagccc ttccagtcgg caatgtccat
     2941 cgcccgccgc atatgccctt cgaattcgcc gctgtcgtag acgcggtcca tcggcgtgcg
     3001 atagggaaac tgcgacggct tgatgaaatt gcgggcgcgc acccgatccg gcgtcgtgcc
     3061 ggtctcaagt gcgatgcgat cgacaaggcg ctcgatcaga taggctgctt ccggccgccc
     3121 ggcgccacga taggcatcga ccggcacggt atgggtatag atgccgcgaa cccggaaata
     3181 ggcctgcgga atatcgtagc agccggtcgt catcgtgccg ccgagatagg gaatataggg
     3241 cgcatattgc gacagatagg cgccgaaatc ggccttcaga tcgacgcgca tggcgagaaa
     3301 ccgcccggat ttcgacaatg ccatctcggc ggtcacgaga ttgtcgcgcc cctgcgcgca
     3361 ggagaggaaa tgctccgtgc gctcctgcac ccagcagacc ggcttcttca gcacccgcgc
     3421 ggccttggcc agcagcggat actcgcgata catgaaggcc ttgttgccga agccgccacc
     3481 gacatccggc gtgacgacac ggatcttctc cggcttctcc ttgaggatcc tggccagcgt
     3541 atcgcgcagg ccatggctgc cctgactacc cagtgtcagc agatactgcc ttgattttgc
     3601 gtcatattcc gccgtgcaga cgcgcggctc catgtaattg gcaaccaacc ggttgttgac
     3661 cagcgtgacg ctcaccaccc gatccgcctt ggcaaaggcc gcatcggtcg ccttggcatc
     3721 gccgattgcg gtatcgagcg cgacattgcc ctcgacatcg gaccatacct gcggcgcgcc
     3781 gggcagcatc gccgccgcga tccccaccgc cgccggcaat tcctcgtaat gcacctcaag
     3841 cgcctcggcg gcgtcctttg cctgctccag cgtggaggcg acgatgaagg cgaccgcatc
     3901 gcccacatgc cgcacctcgc ccctggccag cacggcatag tcggtggcga ccatgcgctt
     3961 gccattgagg ttgcgttgca gcgcctgaca cggcagcggc ccgagatcgg cgacatcggc
     4021 ggcgctccag gcaaggcgca cgcccttcat cgcgcggaca tcgtcgatgt tggcgatggt
     4081 gaatgtggca tgggcatgcg gcgaacgcac cagaaccgcc gtcagcgccc cctccggccg
     4141 ataatcggtg gcatagtggc cggcgccggt gatgaagcgc tgatcttcct tgcgcctgac
     4201 cgcctggccc atcccgaatt tcatcggccg catacccgtt ctcctcgcaa gattgcgcgg
     4261 gcctcagccc gccctgatcg cctcgccggc catcacccag tcacgaaagc cgccgcgata
     4321 atgttcgttg agggacagtc cctcggcctg caccaccgcg atcgcatgct ggctgcgcac
     4381 gcctgccgcg caggagaaca cgattcgctt gtccttgaac ggggcaagat cggccgcgct
     4441 cagccgcgac agcggcgccg agaccgaacc gggaacatgc ccggcggcaa attcgtgatc
     4501 ctcacgcaca tcaaccagca ccagcgaccc gtcggccatg ccggctttca cctcactgcg
     4561 gctgacctcg cggatggcat tcacgggttt ggttcctcaa gcggcagccg gctggccggc
     4621 ggttgtcaga caaggtatgc atctcgcggc gccggatcaa ccggcatgat ggcgcaacgg
     4681 ttcgcgcgaa aaggaaagcc ctcgcatgct gctggaagga tcgcaactct ggttcggcct
     4741 cgtcggcgcg ctgctgatcg gcctcggcaa gggcggcctc ggctccggct ttggcatgct
     4801 ggtggtgccg gtgctgtcgc tgacgctcga tccgcgccag gcagcggccc tgacattgcc
     4861 tattctcatc acttccgact tttttgcggt ctatgcctat cggggcaagg cacataccgg
     4921 ccatatgcgg gtgctgctga ccggcgcggt gatcggcatc gggcttggcg ctctgacctt
     4981 tcacctcgtc tccgaaccga tgctgcggct gatgatgggg ctgatggcgc tgatctatgt
     5041 cgtctcgcgc tttctcggtc ttgcgcgccc ttcggacgtg ccgctgcccc ccgattgggg
     5101 ccagggcgtg acgctcggca cgatttctgg cttcaccagc ttcaccgccc atgccggcgg
     5161 gccgccggtg caggtctaca tgttgcccaa gggcctcgac aaacaggcct tccaggccac
     5221 caacgtgctg ctgttcacgc tgatcaacct catcaaggtg ccgccctatc tggcgctcgg
     5281 ccagttcaca ggcccggtga tgctgaccag cctgatgctg atgccggttg ccgccggcgg
     5341 cgtgtttctc ggctatcggc tgcatcgctg gatgccggaa acgctgttct tccgcattgc
     5401 tgtgacgctg ctgttcctga ccggcctcaa gctgacctgg gatggcctcg gcgccctgct
     5461 gttctgagag cgtcgaattg ccgcttgaaa cgcacccggc cctgcgcttc cctgtagcga
     5521 taaaacggga ggcgatgcct atgagactga agggcaaggt ggcgattgtc accggcggag
     5581 cgcaggggtt cggcgagggg atcgtgcgcg cctttctcgg cgagggcgca aaggtcgcca
     5641 ttctcgacat caagacggcg gaagccgccg cgctcgccaa agagcttggc aaggatacgg
     5701 ctgccttcgc ctgtgatgtg gcgtcgctgg cctcggtgcg cgaggcggtg gccgcagtcg
     5761 taaggcattt cggccggctc gatatcgtgg tcaacaacgc cggctggact tactccaaca
     5821 agccggcgac cgagaccacc gaagcggaat tcgacaaggt gttcgccgtc aacgtcaagt
     5881 cgatctacaa tttcaccgct acagccaccg agcatcttgc cgcgacgcaa ggctcgttca
     5941 tcaacatcgg ctcgaccgcc ggcatccgtc cgcgccccgg cctggtctgg tataatgcca
     6001 ccaagggcgc cgtgcacaac gccaccaagg ttctggcggt ggaactggcg ccgaggaaga
     6061 tccgcgtcaa cgccatcgcc ccggtcgccg gcgaaacgcc gctgctggcg agcttcatgg
     6121 gcacggatac gccggagcgg cgcgcccagt ttctcgccac cattccgctc ggccgctttt
     6181 cccagcccag ggacatcgcc aacgcggcgg tgtttctcgc ctcagccgaa tccgaattca
     6241 tcaccggcgt cgtccttgag gtggacggcg gccgctgtgt ctgagcggtg acagcaccat
     6301 gaccgccgcc gcgcccggcc ccgacatgcc gccgacaccg gcggaccgag cgcgttttgc
     6361 tggtttgcag gcggatttcg cccgttcctt cgcccgtctg gtgactgacc ccaatgcgcc
     6421 gcgcaccgtc gtcatcctgc ccagcctcag cctcgatgcc gatgtgctcg cccgcatttc
     6481 cggcgtgcat cattacgagg agcggatgct gtgcctgctg ctgctgctgc gcatgccgcg
     6541 aaccgaggtg atctacctca cctcgatgcc tatccccgac actatcatcg actattatct
     6601 ccacctgctg ccgggcattc cgatgcagca tgcgcggcgg cggctgacgc tgatcgcctg
     6661 cgacgatgcc tcgcccgtcg cgctcagccg caaactgctg gcccgccccc gcctgctgtc
     6721 ccgcatcggc gaatccatcc gccaccccaa ggatgcgcat ctgagctgct tcaatgtcac
     6781 cgacgatgag cgccggctgg cgctggcgct caacctgccg atctacggct gcgacccgga
     6841 cctggcgcat tggggatcga aaagcggctc ccgcaaggtg ttccgcgccg ccgggctcga
     6901 catgccggca ggcgccgagg acctgcgcga ccagcaggat atcgtcgatg cacttcttga
     6961 cctgaagcgc cggaaacccg gccttcgccg cgcggtcgtc aagctcaacg agggcttttc
     7021 aggcgagggc aacgccacct tccgctttga tggcgcaccg gagggcgcca gcctcgcacc
     7081 ctggctgaag ggccggctgc cgcatctcgc cttcgaggcc agcggaatga cctgggacct
     7141 gttccgcagc aagttttccg cgatgggcgg catcgtcgag gaatgggtgg aaggcgcggc
     7201 caaacgttcg ccctccgcgc agatgcgcgt cgatcccggc ggccagccac aggcgatttc
     7261 gacccatgat caggtgctgg gcgggccgag cggacagatc ttcctcggcg ccaccttccc
     7321 cgccgatgcg gcctatcgca agacggtgca ggacgccggc ctggccgccg catcggtgct
     7381 ggcggagaaa ggcgtgatcg gccgtttcgg cgtcgatttc ctgtccgtcc cggagggcgg
     7441 tggctggagc cacaaggcca tcgagatcaa cctgcgcaag ggcggcacca cccacccttt
     7501 cctgatgctg caatttctca ccgacggcac ctacgacacc gagagcggcc agtttcacgc
     7561 cgctgccggc cagccccgct gctactacgc caccgacacg ctggaagcga aacgctaccg
     7621 cggcctgacg ccggcggacc tcatcgacat tgccgcgctg aacggcctct atttccacgc
     7681 cggccagcag gaaggggtga cattccacct gatcggcgca ctgtcggagt tcggcaagct
     7741 gggcgtcgtc gctatcggcg ccgaccccgc ccgggccgac gcgctctacc gccgcacggt
     7801 tgccgcgctc gaccgcgaaa ccgcttgaag ccacggactc aaatcctcaa caccgcccgc
     7861 caaccgattc aaaggactcg gcttttcgca cgcacttcat gttgactcac aacatgaact
     7921 ttctaccgaa ccataggagc aacgcgacgc cgagctgatg cgaggggccg cccttgccag
     7981 cggcaagggc aaccaaaaaa accctgcgaa gcgacgccga gctgatgcga ggggccgccc
     8041 ttgccagcgg caagggcaac caaaaaaacc ctgcgaagcg acgccgagct gatgcgaggg
     8101 gcgcccgcgt cagcgggcca ctcaagaccc agccagatgg catgccacct tgcgcccgcc
     8161 gtggtcgtgc agcggcggca cctccgtccg gcagcgatcg gccgcgaacg ggcagcgccc
     8221 ggcaaagccg cagccgcctg aagccttgcc gccatcatcc ctgaggcgca cgcgttcgcg
     8281 ggcttcgttc gcatcgatgc ccggcaccgc gtcgaccagc gcccgcgtat agggatgggc
     8341 gggattgtcg aacaccgaag aggtctcgcc atactcgacc acacggccgc gatagagcac
     8401 cagcacgtca tcggcgacat gctcgaccac cgcaagattg tggctgatga acagataggt
     8461 caggccgcgc gcctctctga gatcggccat caggttgagc acctgcgcct gcaccgacac
     8521 atcgagcgcc gagaccggct catcggcaat caccagcttc ggtccggtga tcaacgcccg
     8581 cgccagtgcc acgcgctgcc gctgcccgcc ggagaactca tgcgggtaac ggtcgagcgc
     8641 ctcgcccggc agcgccacgc tgtcgagcat cgccgcgacc cgctgacgcc gctctgccgc
     8701 cgtcagctcc ggctcggcca cttccagcgg ctcggcaatg gcatcgacaa tcttcatccg
     8761 cggatcgagc gaggagaacg gatcctggaa caccatctgg aaatgccgcc gccgcgcccg
     8821 caggtcagcc tcgtccagcc cgttgaggtc ctcaccggca aaggcgatcg tgccagaatc
     8881 cggccgctcc agcgccatca ccagccgcgc cagcgtcgac ttgcccgatc ccgactcccc
     8941 gacaatgccc atcgagcggc cagcctgcac agtgaacgac acatcatcca ccgccgtcag
     9001 catcggcacc ggcccggaca gcctggtccg cttgagcgga tagcggcgtg tcaccgcacg
     9061 cgcttcgacc agaggcgcgc tcaatggatc gcctcccccc gatggcaatg caccagcgag
     9121 gcgcccgcc
//
LOCUS       DAOMXG010000096         9117 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_4019_length_9117_cov_10.132863, whole genome shotgun sequence.
ACCESSION   DAOMXG010000096 DAOMXG010000000
VERSION     DAOMXG010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 9117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9117
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4019_length_9117_cov_10.132863"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            225..494
                     /locus_tag="PLQ11_07690"
     CDS             225..494
                     /locus_tag="PLQ11_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24820.1"
                     /translation="MFKFRPGFLTLAVPLALGACTVETFYAPISVEIVPADERAIVIR
                     NLNPPEDCRIIRQDALFPAIYSVVYGPASPDAASRWASRNCRGAR"
     gene            complement(571..1053)
                     /locus_tag="PLQ11_07695"
     CDS             complement(571..1053)
                     /locus_tag="PLQ11_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HRK24821.1"
                     /translation="MDAAEHAAHRAAITAGLRETTGINEAMIECVVHEFYARVRQDPF
                     IGPVFEARIHDWEAHYDQMCAFWSSVTLQTGIYSGRPMPKHAVLPVDAKHFDRWLQLF
                     GETVDDLCPPRAARHFMDRAQMIAKSLEGGVAMAAGRTLRNGERYQASAPAVLETQVA
                     "
     gene            1490..2107
                     /gene="rpsD"
                     /locus_tag="PLQ11_07700"
     CDS             1490..2107
                     /gene="rpsD"
                     /locus_tag="PLQ11_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HRK24822.1"
                     /translation="MTKRVDAKHKIDRRMGENIWGRPKSPLNRREYGPGQHGQRRKGK
                     LSDFGTQLKAKQKLKGYYASITERQFRKYYAEAVRMKGDSGENLIGLLERRLDAVVYR
                     AKFVPTVFAARQFVNHGHVKVNGRRVNIGSYLVKPGDVVEVKDASREMLMVLETRQLA
                     ERDVPDYIEVDGSKPIAKLTRIPTLSEVPYPVRMEPNLVVEFYSR"
     gene            2247..2450
                     /locus_tag="PLQ11_07705"
     CDS             2247..2450
                     /locus_tag="PLQ11_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24823.1"
                     /translation="MWKLFALIHLLGATILSGVLVLIVLATPSLADSHMKLVPAAFIV
                     GILLAIPPSLWATKAVLAQTKGQ"
     gene            complement(2595..3119)
                     /locus_tag="PLQ11_07710"
     CDS             complement(2595..3119)
                     /locus_tag="PLQ11_07710"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010971907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRK24824.1"
                     /translation="MVAGLDNSGPEHWQSRWEQRLNTARRVHQRDWLKPVKDEWVASL
                     VRAVAAARQPVILVGHSLGVATIVHAAPLLPKGDVIGAFLVGPTGDTAIEREDAIDRH
                     FMPVPRVKLPFPAVVVASHDDPYCPFLTAEEYASAWGAEFVDAGHSGHINTASGHGPW
                     PEGLMRFAGFLAKL"
     regulatory      3285..3390
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3442..5328
                     /gene="thiC"
                     /locus_tag="PLQ11_07715"
     CDS             3442..5328
                     /gene="thiC"
                     /locus_tag="PLQ11_07715"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HRK24825.1"
                     /translation="MTIHQKSIQPGPNTVTTGPICGSRKVYAAPASHPQLRVPFREIA
                     LSDPNEAPVRVYDPSGPYTETDAAIDLDKGLPQVRAGWIAARGYGAIEPRAVTLADNG
                     GATGDKLVPACPAERIVRAAAPGQMVTQYEFARAGIITEEMVYVAHRENLAREAALAG
                     AKERMADGEHFGASIPEFMTPEFVRDEIARGRAIIPANINHTELEPMAIGRNFLVKVN
                     ANIGNSAVTSSAAEEVEKLVWAIRWGADTVMDLSTGRNIHNIRSWILRNSPVPIGTVP
                     IYQALEKVGGDPAKLDWEVFKDTLIEQAEQGVDYFTIHAGVRLAYVPLTANRVTGIVS
                     RGGSIMARWCLSKHRESFLYERFGDICDIMRKFDVSFSLGDGLRPGSVADGNDAAQFA
                     ELETLGELTKIAWDKGCQVMIEGPGHVPMHKIKANMDKQLKECGEAPFYTLGPLVTDI
                     APGYDHITSGIGAAMIGWFGCAMLCYVTPKEHLGLPNRDDVKTGVITYRIAAHAADLA
                     KGHPAAKIRDDALSRARFDFRWEDQFNLGLDPDTARAYHDETLPKDAHKVAHFCSMCG
                     PKFCSMKITQDLRKETQAMGENEKASLAAIAAEAEAGMKAKAAEFAAIGGRLYVDAAK
                     PAAE"
     gene            5507..5956
                     /locus_tag="PLQ11_07720"
     CDS             5507..5956
                     /locus_tag="PLQ11_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24826.1"
                     /translation="MSRSMKRRGSRYACGKAPVAELCSRLAITAVEMPSLAPLMELAE
                     STIRAQVQRAELMQAELERLRNAVTQQHGGHSRTPAFMHGVRHAEKRMQTWLHERAEW
                     MNDPNATSVLHSAAFDLGGLKWGWIKQLHRMNKARDKMNKLRASSCD"
     gene            complement(6056..7459)
                     /gene="purB"
                     /locus_tag="PLQ11_07725"
     CDS             complement(6056..7459)
                     /gene="purB"
                     /locus_tag="PLQ11_07725"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003602995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HRK24827.1"
                     /translation="MIPRYARPEMVAIWEPQNRFRIWFEIEAHATDKLADLGVVPKAA
                     AAKIWEKGRNATFDVARIDEIERVTKHDVIAFLTHVAEIIDDADASRFLHQGMTSSDV
                     LDTCLNVQLTQAADILLKDLDALLAAIKRRAYEHKMTPTIGRSHGIHAEPVTFGLKLA
                     QAYAEFMRNRERLVAARKDIATCAISGAVGTFANIDPRVEAHVAEKLGLAVEPVSTQV
                     IPRDRHAMFFATLGVIASSIERLAIEIRHLQRSEVYEAEEYFSEGQKGSSAMPHKRNP
                     VLTENLTGLARMVRSYALPAMENVALWHERDISHSSVERYIGPDATITLDFALARLTG
                     VIDKLLVYPKNMQKNLDMLGGLVHSQRVLLALTQKGVSREDSYRLVQRNAMPVWRGEG
                     NFLELLKKDKEVNARLSDAELEALFDLGYHLKHVDTIFARVFDEAHQHTSSEKRKAAV
                     EAAEETLGRIATILPHV"
     gene            complement(7663..8319)
                     /gene="rpe"
                     /locus_tag="PLQ11_07730"
     CDS             complement(7663..8319)
                     /gene="rpe"
                     /locus_tag="PLQ11_07730"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017487096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRK24828.1"
                     /translation="MSLKIAPSILAADFANLGPDVAAVDAAGADWIHIDVMDGHFVPN
                     ITFGPELVKAIRPYTKKLFDVHLMIAPVDPYIEAFAKAGADLISFHVEAGAHSHRTLQ
                     LIRSLGKKSGIVLCPGTPASTIEPLIDMLDLVLVMSVNPGFGGQSFIRSATKLIADCK
                     ALIGDRPIDLEIDGGITPETAPLAVQAGANVLVAGSAVFKGGRDALARNIAAIRAAAN
                     "
     gene            8421..>9117
                     /locus_tag="PLQ11_07735"
     CDS             8421..>9117
                     /locus_tag="PLQ11_07735"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRK24829.1"
                     /translation="MHSDVIILGAGIVGLGVALNLQQRGRSVILVDRGGPGEGTSFGN
                     AGLIQREAVLPYHFPTDPLTILTAALNLRNDSRLHWGSLGKIAPFLLAYARQASKASI
                     LRSAAARAPLLAIAMEEHEKLADAAGARLQLRPGAWLKVFRKEKTLADEIAKVNQLEA
                     YGVKGLILDHREVAAAEPHITAHVIGGVRWTQGYNLADPLALSQAYADLFAARGGQVL
                     TGEARSLAATGGGW"
BASE COUNT         1678 a         2750 c         3028 g         1661 t
ORIGIN      
        1 gcctcggggc gcggcgtgct ggcgcagccg gcaagcgcca gaacaaccag cagaaggaca
       61 gcaggcaaac gcatcagcac aggtctcgaa ctcactcgga taggcgcggg cggacgaacc
      121 tgattacacc ggaacggggt ttgcggaaag actgcatgct ggccagggcc tgccgcgctg
      181 cgatagtgcc atactgatgc cgttttcgcg gcgttgctgg cagcatgttc aaattcagac
      241 ccggttttct cactctggcc gttccgctgg cgctgggcgc ctgcacggtg gaaacctttt
      301 atgcgccgat ttcggtcgaa atcgtccctg ccgatgagcg cgccatcgtc atccgcaatc
      361 tcaacccgcc agaagattgc cggattatcc gccaggatgc gctgttcccg gcgatctatt
      421 ccgtggtcta cggcccagcc agtcctgatg cggcgagccg ctgggccagc cgcaattgcc
      481 gcggggcacg ttgaggataa ataaaaaggc gccgcccgtg gcgacgccct gatcttttgc
      541 cgcgacagag cctgtcaggc gtgccggatt tcaggcaacc tgggtttcca gcacagccgg
      601 ggcgctggcc tgatagcgct cgccattgcg cagggtgcgg ccagcggcca tcgcaacacc
      661 gccctcaagg ctcttggcga tcatctgcgc acggtccatg aaatggcgcg cggcgcgcgg
      721 cggacaaaga tcatcaaccg tctcgccaaa caattgcagc cagcgatcga aatgctttgc
      781 atcgaccggc agaacggcgt gtttgggcat cgggcggccc gagtagatgc cggtttgcag
      841 cgtcaccgac gaccagaagg cacacatctg gtcataatgg gcttcccaat cgtggatgcg
      901 ggcctcgaac accgggccaa tgaaggggtc ctgccgcacg cgggcataga actcgtgcac
      961 cacgcattcg atcatggcct cattgatgcc ggtcgtctcg cgaagcccgg cagtgattgc
     1021 agcccgatgg gcggcatgtt cggcagcgtc catagtcgtc tcctgtgcaa tgcaacaact
     1081 acccgatttt gtgttgcggt gcaatataaa atccggccac atgctctgcg ttctaacgca
     1141 aattgattgc caaccgcttg cagaacgcgg ttaagggggc gtttttatgg cgacgcagcg
     1201 gaatgcgttt gctgcgctgc gacaggccgg tcaaggagaa atacccctaa ggccaccacg
     1261 gggcgctgaa aaagtaggcg tccggtttga caagggccgg atttgcgatt atcccgcccg
     1321 ccatgacgcg gggccttcat ggctccgcgc tcatttttga cgcacccgtg gttcgctcag
     1381 cacgatcgaa cctgtcggcc gccgcaaggt ggcagaggag ggcgcgttcc tcacccggcc
     1441 gggctgacga ggcaccggtc ccgtgtttgt ttgtgaaaga ggaaccgcga tgaccaagcg
     1501 cgtggatgcc aagcataaaa tcgatcgccg tatgggcgag aatatctggg gccggccgaa
     1561 gtcgccgctc aaccgtcgtg aatacggccc gggccagcat ggccagcgcc gcaagggcaa
     1621 gctctcggac ttcggcaccc agctcaaggc caagcagaag ctcaagggct attatgcctc
     1681 gatcaccgag cggcagttcc gcaagtatta cgccgaagcc gtccgcatga agggcgactc
     1741 gggcgagaac ctgatcggcc tgctggagcg ccgtctggat gccgtggtct atcgcgccaa
     1801 gttcgtgccg accgtgttcg ccgcgcgcca gttcgtcaac catggccatg tcaaggtcaa
     1861 cggccgccgc gtcaacatcg gctcctatct ggtcaagccg ggtgacgtcg tcgaggtcaa
     1921 ggatgcctcg cgcgagatgc tgatggttct ggaaacccgc cagctcgccg agcgtgatgt
     1981 gcccgattac atcgaggtcg atggttccaa gccgatcgcc aagctcaccc gcatcccgac
     2041 gctttcggaa gtgccgtatc cggtgcggat ggagcctaac ctggtggtcg agttctactc
     2101 gcgctgattg gccgcaggct tttcgaggtc agaaggccgc cttcgggcgg cctttttgtt
     2161 tgacgcaggt caagcgcgtt gcgctgcatc aaggcaggtt ctgccatgcc gaatatgcat
     2221 ggaccccgcg atatggaggt tctgttatgt ggaagctttt tgctctcatc catctgcttg
     2281 gggcgacgat cctgtccggc gttctcgtgc tgatcgtctt ggcgaccccg tcgctggctg
     2341 acagccatat gaagctggtt ccggctgcct ttatcgtcgg tatccttctg gcgattccgc
     2401 cgagcctctg ggcaaccaag gccgtgctgg cgcagaccaa ggggcagtaa cccgccctcg
     2461 ggtggttgtt gcccgccgcg agcccttgcc tgggtccata tccgtggcaa gggcggcttt
     2521 tgtgccgctc gggttgaatg gcacgccgct gacggcggct tttgccgcag tcggaaggcg
     2581 ggatttgagc cggctcacag cttggcgagg aacccggcaa accgcatcag cccttccggc
     2641 caggggccgt gaccgcttgc ggtgttgatg tggccggaat ggccggcatc gacaaattcc
     2701 gcgccccagg ccgaggcata ttcttctgcc gtcaggaacg ggcagtaggg atcgtcatgg
     2761 ctggcgacga caacggcggg gaagggcagc ttcacccgcg gaaccggcat gaaatgccgg
     2821 tcgatcgcgt cctcgcgctc gatggcggtg tcgccggtgg ggccgacaag gaatgcgccg
     2881 atcacgtcgc ccttcggcag caggggcgcg gcgtggacga tagtggcgac gccgaggctg
     2941 tggccgacca gaatgaccgg ctggcgggcg gcggcaacag ccctgacaag gcttgccacc
     3001 cattcatcct tcaccggctt cagccagtcg cgctggtgca cgcgccgcgc cgtgttcagt
     3061 cgctgttccc agcgtgattg ccagtgttcg ggtccggaat tgtccagacc ggcaaccatc
     3121 agaatatcga cgtcgcttgt gcgtttggcc aagatgcgct cccctcaagg cgcagttctg
     3181 ccaaagctca ggtgcgggag caagcgggaa gcggggcgcg tggtaaaata agcggcaagg
     3241 gcgcttgtcg gccttgactt atgcctcacg ctgtccaacg taaggtccgt tccaagggga
     3301 gccttatctg gctgagatgc gcggcaatgc gcggaccctt cgaacctgat ccgggtcatg
     3361 ccggcgaagg gatcggaacc agcgctgtgt gcgccttttc cacccgaccg tccggcgagc
     3421 ccatgagcga gagggacggc catgaccatt caccagaagt ccatccagcc gggaccgaat
     3481 acggtcacca ccgggccgat ctgcgggtcg cgcaaggtct atgccgcgcc ggcgtcgcat
     3541 ccgcagcttc gcgtgccgtt ccgcgaaatc gcgctgagcg atcccaacga ggcgccggtg
     3601 cgggtgtatg atccgtccgg cccctatacc gagaccgacg cggcaatcga cctcgacaag
     3661 ggcctgccgc aggtgcgcgc cggctggatc gcggcgcgcg gctatggcgc catcgagccg
     3721 cgtgcagtca cgctggccga caatggcggg gcgaccggtg acaagctggt gcctgcctgc
     3781 ccggcggagc ggatcgtccg tgccgccgct ccgggccaga tggtgacgca atacgaattc
     3841 gcccgcgccg gaatcatcac cgaagagatg gtctatgttg cgcatcgcga gaatcttgcc
     3901 cgcgaggcgg cgctggccgg tgcgaaggag cggatggcgg atggcgagca tttcggtgcc
     3961 tccattcccg aattcatgac cccggagttt gtgcgcgacg agatcgcgcg cgggcgggcg
     4021 atcattccgg ccaacatcaa ccacaccgaa ctcgaaccga tggcgatcgg ccgcaatttc
     4081 ctggtcaagg tcaatgccaa tatcggcaat tcggccgtga cctcgtcggc ggccgaggaa
     4141 gtggagaagc tggtctgggc catccgctgg ggcgccgata cggtgatgga cctgtcgacc
     4201 gggcgcaaca tccacaacat ccgctcgtgg atcctgcgca attcccccgt tccgatcggc
     4261 acggttccga tctatcaggc gctggaaaag gtgggcggcg atccggccaa gctcgattgg
     4321 gaggtgttca aggacacgct gatcgagcag gcggagcagg gggtggatta tttcaccatc
     4381 catgccggtg tgcgcctcgc ctatgtgccg ctgacggcca accgggttac cggcattgtg
     4441 tcgcgcggcg gctcgatcat ggcgcgctgg tgcctgtcca agcacaggga atcgttcctc
     4501 tacgagcgct tcggcgacat ttgcgacatc atgcgcaagt ttgacgtgtc attctcgctc
     4561 ggcgacgggc tgcgtcccgg ctcggtggcg gacggcaacg atgcggcgca gttcgccgaa
     4621 ctcgaaaccc tcggagagct gacgaaaatc gcctgggaca agggctgtca ggtgatgatc
     4681 gaagggcccg gtcatgtgcc gatgcacaag atcaaggcca atatggacaa gcagctgaag
     4741 gaatgtggcg aggcgccgtt ctacaccctc ggcccgctgg tgaccgacat cgcgcccggc
     4801 tacgatcaca tcacctccgg tatcggcgcg gcaatgatcg gctggttcgg ctgcgccatg
     4861 ctctgctacg tcacgcccaa ggagcatctc ggcctgccca atcgcgacga tgtgaagacc
     4921 ggtgtcatca cataccgcat cgccgcccat gcggccgatc tcgccaaggg ccatccggcg
     4981 gcgaaaatcc gcgacgatgc cttgtcacgg gcgcggtttg atttccgctg ggaggatcag
     5041 ttcaatctcg gcctggaccc ggacaccgcc cgcgcgtacc atgacgagac cctgcccaag
     5101 gacgcgcaca aggtggcgca tttctgctcg atgtgcgggc cgaagttctg ctcgatgaag
     5161 atcacccagg acctgcgcaa ggaaacgcag gcgatgggcg agaacgaaaa agcctcgctc
     5221 gcggcgatcg cggcggaggc cgaggcgggc atgaaggcca aggcggcaga gtttgcggcc
     5281 atcggcggcc ggctatatgt ggatgcggca aagcccgcag cggagtgagc gcaccgccgg
     5341 cggggcaagc gcaatcgaaa actccctccc ccttgcgggg agggtggtct cgggtcgttc
     5401 tgcgtgtcgc tccaccccca ccccgccgcc ggggcgtcga ccctccccgc aagggggagg
     5461 gtgaggggag cgacgactgg gtttgaggtt gcaggagaaa tttgccatga gccgttcaat
     5521 gaagaggcga ggcagccgat atgcgtgcgg caaggctccg gtcgctgaac tttgtagccg
     5581 ccttgcgatc acggcagtcg aaatgccgtc attggcacca ctgatggagc tggctgaatc
     5641 gactattcgg gctcaggttc aacgagcgga attgatgcag gcggagttgg agcgtcttcg
     5701 gaacgcggta actcaacagc acggcggaca cagtcgaacg ccagctttca tgcatggtgt
     5761 gaggcatgcg gaaaaacgaa tgcaaacgtg gctgcatgag cgtgcggaat ggatgaacga
     5821 tccaaacgcg acgtctgtcc tgcattcggc agcattcgat cttggcggcc tcaagtgggg
     5881 gtggattaaa cagctccacc gtatgaacaa ggccagagac aagatgaata aactcagagc
     5941 ctcatcttgc gattaaacct gaaattcgag cagaaatgcg aatcatttga atcggttgcg
     6001 acgtaatttt cgcaatccga ttccgattgt tgaagcttcg cgctgccttg aaatctcaga
     6061 catgcggcag gatggtcgcg atgcgcccga gcgtttcttc cgccgcctcc accgcagctt
     6121 tccgcttctc ggaggacgtg tgctggtggg cttcgtcgaa cacccgggcg aagatcgtat
     6181 cgacgtgttt gaggtgatag ccgaggtcga acagcgcttc gagttccgca tccgacaggc
     6241 gggcattgac ctccttgtcc ttcttcagaa gctcaaggaa gttgccctcg ccgcgccaga
     6301 ccggcatggc gttgcgctgc accagccggt agctgtcctc gcgggaaacg cccttctggg
     6361 tcagcgccag cagcacgcgc tgggaatgga caaggccgcc gagcatgtcg aggttcttct
     6421 gcatgttctt tggatagacc agcagcttgt cgatgacgcc ggtgagacgt gccagcgcga
     6481 agtcgagcgt gatcgtcgca tccgggccga tgtagcgctc gacggaggag tgggagatgt
     6541 cgcgctcgtg ccagagagcg acattctcca tcgccggcag ggcatagctg cgcaccatgc
     6601 gggcaaggcc agtgaggttc tcggtcagca ccgggttgcg cttgtgcggc attgccgagg
     6661 agcccttctg cccctcggag aagtattcct ccgcctcgta gacctccgag cgctgcaggt
     6721 ggcggatctc gatcgccagc cgctcgatgg atgacgcgat gactccaagc gtggcgaaga
     6781 acatcgcatg gcggtcgcgc gggatcacct gggtcgacac cggctcgaca gcgaggccca
     6841 gcttttcggc gacatgggct tcgacgcgcg gatcgatgtt ggcaaaggtg ccgacggcgc
     6901 cggagatggc gcaggtggcg atgtccttgc gggcggcgac gaggcgctcg cggttgcgca
     6961 taaattcggc ataggcctgc gccagcttga ggccaaacgt caccggctcg gcatggatgc
     7021 cgtggctgcg gccgatggtc ggcgtcatct tgtgttcgta ggcgcggcgc ttgatggcgg
     7081 cgagcagcgc atcaaggtct ttgagcagga tatccgccgc ctgggtgagc tggacgttga
     7141 ggcaggtgtc gagcacatcc gaggaggtca tgccctgatg caggaagcgc gaggcatccg
     7201 catcgtcgat gatttcggcg acatgggtca aaaaggcgat gacatcgtgc ttggtgacgc
     7261 gctcgatttc gtcgatccgg gcgacatcga aggtggcgtt gcggcccttt tcccagatct
     7321 tggcagccgc cgccttcggc accacgccga gatcggccag cttgtcggtg gcatgcgcct
     7381 cgatctcgaa ccagatgcgg aagcggttct gcggctccca gatggcgacc atttcggggc
     7441 gggcgtaacg ggggatcatg gcacaggtcc ttggcaacat ggtcgccctc ccccttgcgg
     7501 ggagggtcgg cgcgtcagcg ccggggtggg ggtggttgcc gcacgacccc cacccgcgcc
     7561 tccgcacgca gtcaagtgtt cgcagactgc gtacacttga ctgctaagct tggaccaccc
     7621 tccccgcaag ggggagggaa gtcgaatgcg tcgcggacac aatcagttcg cggccgccct
     7681 tatggcggcg atatttctgg ccagcgcatc gcgcccgcct ttgaacacgg cggaaccggc
     7741 gaccagcacg ttggcgccgg cctgaactgc gagcggtgcg gtttcgggcg tgatgccgcc
     7801 gtcgatttca agatcaatcg ggcgatcgcc gatcagcgcc ttgcagtcgg cgatcagttt
     7861 cgtggctgac cggatgaagc tctggccgcc gaagccggga ttgacgctca tcaccagcac
     7921 cagatcgagc atgtcgatca agggctcgat ggtggaggcg ggtgtgccgg ggcagaggac
     7981 gatgccggat ttcttgccga gcgagcggat gagctgaagg gtgcggtggc tgtgcgcgcc
     8041 ggcttccaca tgaaaggaaa tcaggtccgc gcccgctttg gcaaaggcct cgatgtaagg
     8101 atcgaccggc gcgatcatca gatgcacgtc gaacagcttc tttgtgtagg ggcggatcgc
     8161 cttcacaagc tccgggccaa aggtgatgtt cggcacgaaa tggccatcca tcacgtcgat
     8221 gtggatccag tcggcgccgg cggcgtcgac agcggcaacg tcggggccga gattggcgaa
     8281 atcggcggcg agaatggagg gggcgatctt cagcgacatg gccggcgctt agcactttgc
     8341 gcccggcggg ggaacaggta ttgcgaagtc aaggatgggc cgcccgcacg gagggatggc
     8401 cggacgcggt ggagcagggc atgcacagtg atgtcatcat actcggtgcg gggatcgtcg
     8461 ggctgggcgt agcgctcaac ctgcaacagc gcgggcgctc ggtgatcctg gtggaccgcg
     8521 gcgggccggg ggagggcaca tccttcggca atgccgggct gatccagcgc gaggcggtgc
     8581 tgccctatca ctttcccacc gatccgctga ccatcctcac ggcggcgctc aatctcagga
     8641 acgattcccg cctgcattgg ggatcactcg gcaagatcgc gccgttcctg ctggcctatg
     8701 cacgtcaggc atccaaagcc agcatcctcc gcagcgccgc tgcgcgggcg ccgctgctgg
     8761 ccattgccat ggaagagcac gagaagctcg ccgatgccgc cggtgcacgc ctgcaattgc
     8821 gccccggtgc ctggctgaag gtgttccgca aggaaaagac gctggccgat gagatcgcca
     8881 aggtgaacca gctcgaagcc tacggggtca agggactgat tctcgatcat cgcgaggtgg
     8941 ccgccgccga gccgcatatc accgcccatg tcatcggcgg cgtgcgctgg acgcaaggct
     9001 acaatctcgc cgatcccttg gcgctcagcc aggcttatgc ggatctgttt gcggcgcgcg
     9061 gcgggcaggt gctgactggc gaggcgcggt cgctggccgc gacgggaggc ggctggt
//
LOCUS       DAOMXG010000097         9108 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_4026_length_9108_cov_9.268751, whole genome shotgun sequence.
ACCESSION   DAOMXG010000097 DAOMXG010000000
VERSION     DAOMXG010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 9108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9108
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4026_length_9108_cov_9.268751"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..293)
                     /locus_tag="PLQ11_07740"
     CDS             complement(120..293)
                     /locus_tag="PLQ11_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24830.1"
                     /translation="MPHRRRQTLGALAVAFIVLALGLWVVGRLDESQRAQACLESGGR
                     RCAGIGKDGTPNR"
     gene            362..613
                     /locus_tag="PLQ11_07745"
     CDS             362..613
                     /locus_tag="PLQ11_07745"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HRK24831.1"
                     /translation="MTTPIAADIAALPFEKALAELEQIVQKLERGEVALEDSIALYER
                     GEALKKRCEALLREAEARIEKLTLGADGAPKASEPLDVD"
     gene            632..1525
                     /locus_tag="PLQ11_07750"
     CDS             632..1525
                     /locus_tag="PLQ11_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HRK24832.1"
                     /translation="MTGMQDGEPVPGEAAAQAGITRIIVPRTSDVGGLAVRRALPAAG
                     ARMIGPFIFFDEFGPHEFLLGQGLDVEPHPHIGLETVTYLFDGEILHRDSLGFAQPIR
                     PGDVNIMTAGRGIVHSERSPEAERQPGKRIHGLQVWTALPLAREEAEPGFRHHGIDEL
                     PRLSGEGKRIRLIMGSAYGEDSPVEAAHPTLYAECVLAPGAILPVDADYDERALYVVS
                     GAIDIAGEQFGAGRLLLLRAGDRISVLALSNARLMLIGGEPMDGPRFIWWNFVASRKD
                     RIDAAKADWSAGRFATVPGED"
     gene            1550..1981
                     /locus_tag="PLQ11_07755"
     CDS             1550..1981
                     /locus_tag="PLQ11_07755"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HRK24833.1"
                     /translation="MLLRAPILSAFVLALAACNTSGTGPSMKWVSFANADLDQRDFSG
                     QNLAGVNFGGAVADGANFARANLEGASFHSAFAEKTLFANANLRRANFSNAHLVGTQF
                     QGADLTGAILDGARMEGADFSAALWVDGKKVCKANSIGSCN"
     gene            complement(2145..2555)
                     /locus_tag="PLQ11_07760"
     CDS             complement(2145..2555)
                     /locus_tag="PLQ11_07760"
                     /inference="COORDINATES: protein motif:HMM:NF013304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HRK24834.1"
                     /translation="MVLVAAILVQVLWTLIVLVMLGRSRAAAVRAGQVKIPDVALSSD
                     AWPDRIKAIGNNYANQFETPVLFYVLAGLALYLGATHAGMALAAWIFVASRIGHTLVH
                     TGGNRVMLRFRIFLIGVAALLAMWLMLALRVAGI"
     gene            complement(2610..3503)
                     /locus_tag="PLQ11_07765"
     CDS             complement(2610..3503)
                     /locus_tag="PLQ11_07765"
                     /inference="COORDINATES: protein motif:HMM:NF016602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24835.1"
                     /translation="MQLFDCFTFFNELDLLDLRLAELDAVVDRFVIGESPLTFTGRSK
                     PLVFAENRARFSRYLHKIEHVVIDDMPNGPETTAWDREWHQRNGLARGLGMAKANDLV
                     MLSDVDEIPRASLLSGVRADPASGNRITILESDLYLYFLNTRPRTRYFSDIQAPRIVP
                     RRAVGKMQTIRAFRARFSRNRLFDPIQPLAARIRALFDFGTPLELKVIANATWHFTYM
                     GGAERVREKVLSYAHTEMQTAENTSAEVLARRIAQREAIVDWGPLDDLPLDASFPAAL
                     LADPARWAHMLAPVQPARRMD"
     gene            3545..4288
                     /locus_tag="PLQ11_07770"
     CDS             3545..4288
                     /locus_tag="PLQ11_07770"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HRK24836.1"
                     /translation="MFDRVLMSLAAGLVKLAKHRKPLWIPYGGTRVLIDSHLITFPIL
                     DMLLRHRYEHFEFEALSILKRRGLLTRADRALELGVGAGVIGLQIASIVGDGHYLGYE
                     ANPAVLDLARRNFALNGMAPDLRRRLLVPGGGGARNFAVSSNFTASALEARADTVGEV
                     SVETDAFEDVLKRFDPTFLVMDIEGGEKEFFAGVGDFGNIRLIMLENHFFDAQRPVFE
                     AIVAKLADHGFREAKDLYTSASVHLFQRG"
     gene            4338..6296
                     /gene="dxs"
                     /locus_tag="PLQ11_07775"
     CDS             4338..6296
                     /gene="dxs"
                     /locus_tag="PLQ11_07775"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006466199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HRK24837.1"
                     /translation="MPVTFGCWNFFALPVSTPILDQVPTPADLRRLEESQLPQVADEL
                     RAEMIDAVSVTGGHLGAGLGVVELTVALHYVFNTPEDRLIWDVGHQAYPHKILTGRRD
                     RIRTLRQGGGLSGFTKREESEFDPFGAAHSSTSISAGLGMAVARDMNGRRNNVVAVIG
                     DGAMSAGMAYEAMNNAGAMHSRLIVILNDNDMSIAPPTGAMSAYLARLGSGKTYLKLR
                     DFGKQLTHKLPRRLDSAITRAVEHARGYVTGGTLFEELGFYYVGPIDGHNLDHLLPVL
                     KNVRDADVGGPVLVHVVTQKGKGYAPAEASADKYHGVVKFDVVTGVQAKPPSNAPSYT
                     KVFAQSLIKEADADPAVVAVTAAMPSGTGLDLFARVHPQRTFDVGIAEQHAVTFAAGM
                     ASEGLRPFCAIYSTFLQRAYDQVMHDVALQNLPVRFAIDRAGLVGADGPTHAGSFDLA
                     YLGCLPNMVLMAAADEAELVHMVRTAAAHDAGPIAFRFPRGDGIGCEMPERGQIIDIG
                     KGRILREGTRVALLSLGTRLQEALLAAEQLAALGLSTTVADARFAKPLDRDLVTRLAR
                     EHEVLITVEEGSIGGFGSFVLQHLADEGLLDRGLRVRAMVLPDVYLDHDKPEKMYARA
                     GLDAKGIVAKVFEALGRDAAAVTRLGIA"
     gene            complement(6477..6866)
                     /locus_tag="PLQ11_07780"
     CDS             complement(6477..6866)
                     /locus_tag="PLQ11_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24838.1"
                     /translation="MGSAKRPVPANLKPRYEPPTLEEALFAAEGLSDNRDEQVAIAAE
                     LMDLPHDRVFHEAEAYFKAQKNRISVPTARHPAGTVIVEKRRSFAPVSLEKRSMAPAF
                     SFERKPAGSKFVVETRAPKRVITLPNR"
     gene            complement(7020..7730)
                     /locus_tag="PLQ11_07785"
     CDS             complement(7020..7730)
                     /locus_tag="PLQ11_07785"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HRK24839.1"
                     /translation="MSTLHRRAFIAAATATLFAPRASAQAPAGPYTRAPLPYAFNALE
                     PHIDAKTMEIHSGRHHEAYVTNMNGFAKDGPEWSKKSITEVLANLNALPEAIRTGVRN
                     NLGGHANHTMFWQIMAANGGKPEGDVLAAIDRDLGGVEKMMTDFNASGLRVFGSGWTF
                     ITVAKDGKLAIVNKPNQDTPIMDGQMVLLGNDVWEHAYYLNYQNRRADYLKAWWNVVN
                     WKAVGARFAAAKAGTLAL"
     gene            7854..8597
                     /locus_tag="PLQ11_07790"
     CDS             7854..8597
                     /locus_tag="PLQ11_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HRK24840.1"
                     /translation="MTVTERRRADVILTERGLFESRSRAQEAIAAGLVRVNGLVLTRP
                     SERIGPDDAITATPVHDYVSRGGLKLAAALAAFGVDPGGRICLDAGASTGGFTEVLLR
                     RGAARVIAVDSGRDQLHARLRGHPAIDLREATDIRALDLGKGQPQPDLAVVDVSFISL
                     KLVLPALACLLAPAAEIVALIKPQFEVGRAAVGKGGIVRDAAAQARAVDEVAAAMEML
                     GFTTARAIDSPVLGGDGNHEFLIHGRRPA"
     gene            8594..>9108
                     /locus_tag="PLQ11_07795"
     CDS             8594..>9108
                     /locus_tag="PLQ11_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRK24841.1"
                     /translation="MSGKRVRGLDARGDGLIDGEARAIAGALPGETIEWDASGRLTTV
                     LVPSPERVTPFCRHFGHCGGCRLQHWAPAASRLWKRQRLVEALDRAGLAPGVDATVDA
                     TGNGRRRVILHLRFDRGRILAGFMAARSHDLVDLDRCPVLVPPLVAAMDVARGLARLL
                     ARRGKPLDVQLT"
BASE COUNT         1588 a         2788 c         2979 g         1753 t
ORIGIN      
        1 gaggaaccgg agcattttca agcgaagtgg tttccggttc gcgtgaagaa aatgcgataa
       61 aacgaggaac cggagcattt tcaagcgaag tggtttccgg ttcgcgtgaa gaaaatgctc
      121 taccggttgg gcgttccgtc cttgccgatg ccggcacagc ggcgtccgcc ggattcaaga
      181 caggcctgtg cccgctggct ttcgtcgagg cgcccgacca cccataatcc gagtgccagc
      241 acaatgaacg cgaccgcgag cgccccaagg gtctgtcggc gccgatgcgg catgccgtgc
      301 tcctgtcaat aacgctcaaa gcgtctgttg ggttgccgga aaatagggta atacgtggcc
      361 catgaccaca ccgattgccg ccgacattgc tgcgctgccg ttcgagaagg ctttggccga
      421 actggaacag atcgtccaga agctggagcg cggcgaggtg gcgcttgagg attccatcgc
      481 gctttacgaa cggggcgagg cgctgaagaa gcgctgtgag gcgcttttgc gcgaggccga
      541 ggcacgcatc gagaaactga cgctgggggc ggatggagcg ccgaaggcca gcgaaccgct
      601 cgatgtcgac tgaccggtcg ggcatgccgg catgaccggg atgcaggacg gcgagcccgt
      661 gcccggcgag gcggcggcgc aggctggcat cacccgcatc atcgtgccgc gcacctccga
      721 cgtcggtggg ttggccgtgc gccgggcatt gccggcagcg ggcgcgcgca tgatcggtcc
      781 gttcatcttt ttcgacgaat tcggcccgca tgagttcctg ctggggcagg ggctggatgt
      841 cgagcctcat ccccacatcg gcctggagac ggtcacctac ctgttcgacg gcgagatcct
      901 gcaccgcgac agcctcggct ttgcccagcc gatccggccg ggcgatgtca acatcatgac
      961 cgccgggcgc gggatcgtcc attcggaacg ctcgcctgag gccgagcgtc agccgggcaa
     1021 gcgcatccat ggcctccagg tctggaccgc actgccactg gcgcgcgagg aggccgagcc
     1081 cggcttccgc catcatggta tcgacgagct gccgcgcctt tccggcgagg gcaagcgcat
     1141 ccgcctgatc atgggcagtg cctatggtga ggactcgccg gtggaggctg cccatccgac
     1201 gctttatgcc gaatgcgtgc tggcgcccgg cgcgatcctg ccggtcgatg ccgattatga
     1261 cgagcgggcg ctgtatgtcg tctctggtgc catcgacatt gccggcgagc agttcggcgc
     1321 cggccggctg ctgctgttgc gcgccggcga ccgcatttcg gtgctggccc tgtccaatgc
     1381 ccggctgatg ctcatcggcg gcgagccgat ggacgggccg cgcttcatct ggtggaattt
     1441 cgtcgcttcg cgtaaggaca gaatcgatgc ggcgaaggcc gactggtcgg ctggccgctt
     1501 tgcgacagtc ccgggtgaag actgatctga tgagcatagg gaaacactga tgctgctgcg
     1561 cgccccgatc ctgtccgcct ttgtcctggc gctggctgcc tgcaacacct cgggcaccgg
     1621 cccgtcgatg aaatgggtca gctttgccaa cgccgacctc gaccagcgcg atttttctgg
     1681 ccagaacctt gccggcgtca atttcggtgg cgcggtggct gatggcgcca atttcgcccg
     1741 ggccaatctg gagggagcga gctttcattc cgcctttgcc gaaaagacgc tgtttgccaa
     1801 tgccaatctg cggcgggcca acttctccaa tgcccatctc gtcggcacgc agtttcaggg
     1861 cgccgatctg accggcgcca ttcttgacgg cgcccgcatg gaaggcgccg atttctcggc
     1921 cgcgctatgg gtggacggaa agaaggtctg caaggccaat tcgatcggca gctgcaattg
     1981 atcggacgac agacgggctg gccgcctgtg cggccagaat gtccgcgcac gatgaaaaat
     2041 tgttagagca tcatcccgcc aggtggatac cggctggcgg aaaaagatga tgcgttcagt
     2101 ataggttaga gcaaaagagc gatccagtca gatcggcttt tgctctaaat cccggccaca
     2161 cgcagggcca gcatcaacca catcgccagc agcgcagcca caccgatgag aaagatgcgg
     2221 aagcgcagca tcactctgtt gccgccggta tggaccaacg tatggccgat gcggctggca
     2281 acaaagatcc aggccgccag cgccatgccg gcatgcgtgg cgccgagata gagcgccaac
     2341 cctgccagca cgtaaaacag caccggtgtc tcaaactgat tggcgtaatt gttgccgatg
     2401 gccttgatcc ggtcgggcca agcatccgag gacagcgcga catccgggat tttcacctga
     2461 ccggcgcgaa ccgcagcggc gcgcgagcga ccgagcatca ccaagacaat caaggtccac
     2521 aacacctgaa ccagaatggc cgcaaccagc accataggag cccccttcgc ccgtcagata
     2581 gtttataaat gaactattct gggtgcttgt caatccattc gacgcgccgg ctgcacgggc
     2641 gccagcatat gggcccagcg ggcgggatcg gccaaaaggg cggccggaaa gctggcgtcg
     2701 agcggcaaat cgtccagcgg cccccagtca acgatcgcct cgcgctgggc aatccgccgg
     2761 gcgagaacct cagccgaggt gttctcggcg gtctgcatct ccgtatgggc ataggagagc
     2821 accttttcgc gcaccctttc ggccccgccc atataagtga agtgccaggt cgcgttggca
     2881 atgaccttga gttccagcgg ggtgccgaag tcgaacagcg ctcttatgcg cgccgcgagc
     2941 ggctggatcg gatcaaacag tcgatttctg gaaaaccgtg cgcggaaagc gcggattgtc
     3001 tgcatttttc cgacggcacg cctgggcacg atccgaggcg cctggatgtc cgagaaatag
     3061 cgcgttctgg ggcgggtatt gaggaaatag aggtagagat cgctctcgag aatagtgatg
     3121 cgattgcccg atgccggatc ggccctgacc ccggaaagca gagaggcgcg cggtatttca
     3181 tccacatcgg acagcatcac caggtcgttc gccttagcca ttccgagccc gcgggcaaga
     3241 ccgttgcgct ggtgccattc ccggtcccat gcggttgttt cgggaccatt cggcatatcg
     3301 tcgatgacaa cgtgctcgat tttgtgcaga tagcgggaaa agcgcgcccg gttttcggca
     3361 aacaccaggg gtttgctcct gccggtgaac gtgagcgggc tttcgccgat gacgaagcgg
     3421 tcgaccaccg cgtcaagttc ggccagccgg agatcgagga gatcgagttc gttgaagaag
     3481 gtgaagcaat cgaatagctg caagagaacc cgtcctccgg tgagatttcc gcgaggaacc
     3541 gcccgtgttt gaccgggttc ttatgtcgtt ggccgccggg ctggtcaagc tggccaagca
     3601 tcgcaagccg ctgtggattc cctatggcgg caccagggtg ctgatcgaca gccacctgat
     3661 cacctttccg atcctcgata tgctgttgcg ccatcgttac gagcattttg agttcgaggc
     3721 actcagcatc ctgaaacggc gcggcctgct gacccgggcc gacagggcgc tggagcttgg
     3781 cgtcggcgcc ggcgtgatcg ggctgcagat cgcctccatc gtcggcgatg ggcattatct
     3841 tggctatgag gccaatcccg ccgtgcttga tcttgcccgc cgcaatttcg cgctcaatgg
     3901 catggcaccg gacctgcgtc gccggctgct ggttccgggc ggcggcggcg cccgcaattt
     3961 cgccgtttcc tccaatttca ccgcctcggc gctggaggct cgggccgaca cggttggcga
     4021 ggtcagtgtt gaaaccgacg ccttcgagga tgtgctgaag cgtttcgatc ccaccttcct
     4081 ggtcatggac atcgagggcg gggagaagga attcttcgcc ggcgtcggtg atttcggcaa
     4141 tatccggctg atcatgctgg aaaaccactt cttcgatgca cagcgcccgg ttttcgaggc
     4201 gatcgtcgcc aaacttgcag accatggctt ccgcgaagcc aaggacctct acacgtccgc
     4261 ttcggtgcat ctgttccagc gcggctgagg gcaggcgccc ctctgtaggc gttgccgtgt
     4321 ggtgcaaacc acgatacatg cctgttacct tcggttgctg gaactttttt gccttgccag
     4381 tctcgacacc cattcttgat caagtgccga cacctgccga tctgcgacgg ctggaagaaa
     4441 gccagctgcc acaggtggcc gacgaattgc gcgccgaaat gatcgatgcg gtgtcggtga
     4501 ccggcgggca tctcggcgca ggccttggtg tcgtcgaact gacggtggcg ttgcattatg
     4561 tgttcaatac gcccgaggat cggttgatct gggatgtcgg ccatcaggcc tatccccaca
     4621 agatcctgac cggccggcgc gatcgcatcc gcaccttgcg gcagggtggc gggctctccg
     4681 gtttcaccaa gcgcgaggag agcgaattcg atcctttcgg cgctgcccat tcctcgacct
     4741 cgatttccgc tggcctcggc atggctgttg cgcgcgacat gaacgggcgc aggaacaatg
     4801 tcgtggcggt gatcggcgac ggcgcgatgt ccgccggcat ggcctatgag gcgatgaaca
     4861 atgcgggcgc gatgcactcg cgtctgatcg tcattctcaa cgacaacgac atgtcgatcg
     4921 ccccgcccac gggagcgatg agcgcctatc tggcgcggct tggttcgggc aagacttatc
     4981 tgaagctccg cgatttcggc aagcagctga ctcacaagct gccgcgccgg ctcgacagcg
     5041 cgattacccg cgcggtggaa catgcgcgcg gctatgtcac cggcgggacc ctgttcgagg
     5101 aactggggtt ttactacgtc ggcccgatcg acggccacaa tcttgaccat ctgctgccgg
     5161 tgctcaagaa cgtgcgcgat gccgatgtcg gcgggccggt tctggtgcat gtcgtcaccc
     5221 agaagggcaa aggctatgcc ccggccgagg ccagcgccga caagtatcat ggcgtcgtca
     5281 aattcgatgt cgtgaccggt gtgcaggcca agccgccgtc caatgcgccg agttacacca
     5341 aggtctttgc ccagtcgctg atcaaggagg ccgatgccga tccggccgtg gtcgcggtga
     5401 cggcggccat gccctcaggc acggggctcg atctgtttgc ccgcgtgcat ccacagcgca
     5461 ccttcgatgt cggtattgcc gaacagcatg cggtgacctt tgccgccggc atggcgagcg
     5521 aagggctgag gccgttctgc gccatctatt cgaccttcct gcaacgggcg tacgaccagg
     5581 tgatgcatga tgtcgccctg cagaacctgc cggtgcgctt tgctatcgac agggcggggc
     5641 tggtcggtgc cgatggccca acccatgccg gttcgtttga tcttgcctat ctcggctgcc
     5701 tgcccaacat ggtactgatg gcggctgccg acgaggcgga actggtccat atggtccgca
     5761 ccgccgccgc ccatgatgcc gggccgatcg ccttccgctt tccgcgcggt gatggcatcg
     5821 gctgcgagat gccggagcgc ggccagatta tcgatatcgg caaggggcgc atcctgcgtg
     5881 agggcacgcg cgtcgcgctg ctgtcgctgg gcacccgcct gcaggaggcg ctgctggctg
     5941 ccgagcagct ggcggcgctg ggcctgtcca ccacggtggc cgatgcacgt tttgccaagc
     6001 cgctcgaccg tgatctcgtc acgcgactgg cgcgcgagca tgaggtgctg atcacggtcg
     6061 aggaaggttc gatcggcggt ttcggctcgt tcgtgctcca gcatctggcc gacgaggggc
     6121 tgctcgatcg cggtctgcgc gtccgcgcca tggtgctgcc ggatgtctat ctcgaccatg
     6181 acaagccgga aaagatgtat gcccgcgccg ggctcgacgc caagggcatc gttgccaagg
     6241 tgttcgaggc gctgggcagg gatgccgctg ccgtcacgcg gctggggatt gcctgaaccg
     6301 gacgctccgg gcaacgccgt gcgccggtca ccgttcggcg cgtttcagga caggtcgatt
     6361 cgtgatacca ccggtcacga gacgtgaccg atgacgaatt ttgaattctc atttttcaat
     6421 ggcaatgaaa attcaaaaca ggaaccaagg gtcaattgtc atgacccttg gtatgatcag
     6481 cggtttggca aggtgatgac gcgcttgggc gcgcgcgttt cgaccacgaa cttcgaaccg
     6541 gcaggcttgc gttcgaacga gaaagccggt gccatgctgc gcttttccag cgacaccgga
     6601 gcaaacgacc gccgtttttc gacgatgacc gtgccagccg gatgccgggc ggtcggaacg
     6661 gaaatgcggt tcttctgcgc cttgaaatag gcctccgcct cgtgaaacac gcggtcgtgc
     6721 ggcagatcca tcagctcggc ggcgatcgcc acctgctcgt cgcggttgtc gctcagccct
     6781 tcggcggcga acagcgcctc ctcaagcgtc ggcggctcgt atcgcggctt gagattggcg
     6841 ggaaccggtc gtttggcgga ccccatgtct gcactctcct gagtttggag agtcaattat
     6901 agcgccaaac attgcgttgc accataaaaa ttttgcagtg caacattaac ctttatgcgg
     6961 ggccggtcga ctgccggcca gaatgcgacg ccgggcggaa aacccggcgt cgccgtggct
     7021 cagagcgcca gcgttccggc cttggcggcg gcgaagcgcg cgccgacggc cttccagttg
     7081 acgacattcc accaggcttt caggtaatcg gcgcggcggt tctggtagtt cagataatag
     7141 gcgtgctccc agacgtcgtt gccgagcagc accatctgcc cgtccatgat cggcgtatcc
     7201 tgattgggct tgttgacgat cgcgagcttg ccgtcttttg ccacggtgat gaaggtccag
     7261 cccgagccga acaccctcag ccccgaggca ttgaaatcgg tcatcatctt ctcgacgccg
     7321 ccgagatcgc ggtcgatggc ggcgagcaca tccccctccg gcttgccgcc gttggccgcc
     7381 atgatctgcc agaacatcgt gtggttggca tgcccgccga gattgttgcg cacgccggta
     7441 cggatggctt cgggcagggc gttgagattg gccagcacct cggtgatcga cttcttggac
     7501 cattccggcc cgtccttggc aaagccgttc atgttcgtca cataggcttc gtggtggcga
     7561 ccggaatgga tttccatggt ctttgcgtcg atatgcggct ccagcgcatt gaaggcgtag
     7621 gggagcggcg cccgcgtata ggggccggcc ggcgcctgcg ccgaggcgcg cggcgcaaac
     7681 agggttgcag tggctgcagc aataaaggca cgacggtgaa gagtgctcat tcgggatact
     7741 ccatcctggt tagaacgcat cgatccccct cggatcttgc atccatgcta gttcgtttct
     7801 aacgaggtcg tcgagtgcct ttgctctgcg gaacggatgc gatggctggg agaatgaccg
     7861 tgacagagcg tcgtcgcgca gatgtgatcc tgaccgagcg cggcctgttc gaaagccgca
     7921 gccgtgcgca ggaggcgatt gccgccggtc tggtgcgggt caacggcctg gtgctgacca
     7981 ggccgtccga gcgaatcggc cctgatgatg cgatcacggc cacaccggtt catgactatg
     8041 tttcgcgcgg gggcctgaaa cttgccgccg cgctggccgc cttcggggtc gatcctggcg
     8101 ggcgcatctg tcttgatgcc ggcgcctcga ccggcggctt caccgaagtc ctgctgcgca
     8161 ggggcgcggc ccgggtaatc gcggtcgatt ccggccgtga ccagctccat gcgcgcctgc
     8221 gcggtcatcc cgccatcgat ctgcgcgagg caaccgacat tcgcgcgctc gacctcggca
     8281 aaggccagcc gcagcccgat ctggcggtgg tcgatgtcag tttcatctcg ttgaagctgg
     8341 tgttgccggc gctggcatgc ctgttggccc ctgcggcgga aatcgtcgcc ttgatcaagc
     8401 cacagttcga ggttggtcgc gccgccgtcg gcaagggcgg gatcgtccgc gatgctgctg
     8461 cccaggcgcg ggcggttgat gaggtggcag cggccatgga aatgcttggc tttaccaccg
     8521 caagagccat cgactcgccg gttttgggcg gcgacggcaa tcacgaattc cttatccatg
     8581 ggcggcgacc ggcatgagcg ggaaacgggt tcggggcctt gatgcgcgcg gtgacggtct
     8641 gatcgacggc gaggcacgtg ccatcgccgg tgccctgcca ggggaaacga tcgaatggga
     8701 tgcctctggc cggctgacca ccgtgctggt gcccagcccc gagcgggtga cgccgttctg
     8761 ccgccatttc ggccattgcg gcggttgccg gctgcaacat tgggcgcccg ccgcttcgcg
     8821 gctctggaaa cgccagcggc tggtcgaggc gctcgatcgg gcagggctcg cgcccggagt
     8881 ggacgcaacg gtcgacgcga ccggcaacgg ccgccgccgt gtgatcctgc atctcaggtt
     8941 cgaccggggg cgtattctgg ccggcttcat ggcggcgcgc agccatgatc tggtcgatct
     9001 cgatcgctgc ccggttctgg tgccgccgct ggtggctgcg atggatgtgg cgcgcggcct
     9061 ggcgcggctg ctggcgcgac gcggcaagcc gctggatgtg cagctgac
//
LOCUS       DAOMXG010000098         9072 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_4052_length_9072_cov_9.824110, whole genome shotgun sequence.
ACCESSION   DAOMXG010000098 DAOMXG010000000
VERSION     DAOMXG010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 9072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9072
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4052_length_9072_cov_9.824110"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /gene="rplO"
                     /locus_tag="PLQ11_07800"
     CDS             <1..356
                     /gene="rplO"
                     /locus_tag="PLQ11_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016917732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HRK24842.1"
                     /translation="VAIKGFEGGQMPLHRRLPKRGFTNIFALDLNEVNIGRVQQAVDA
                     GKLDAKAPVTVDALVAAGVCARARDGVKILGVGEIKAKLSFEVYGASKSARAAIEKAG
                     GSVKLLTEQGEAPQA"
     gene            546..1877
                     /gene="secY"
                     /locus_tag="PLQ11_07805"
     CDS             546..1877
                     /gene="secY"
                     /locus_tag="PLQ11_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HRK24843.1"
                     /translation="MASAAEQLASNLNFGALAKADELKKRIWFTLGALIVFRLGTYIP
                     LPGINPDAVAEIFKSQQNGVLGLFNMFSGGAVGRLAIFALNIMPYISASIIIQLMTTV
                     SPQLEALKKEGEAGRRQINQYTRYLTVVLALFQAYGIAMGLEASGNAVREPGLFFRVS
                     TVLTLTGGTLFMMWLGEQITARGIGNGTSLIIFAGIVAELPAAIANTLELSRQGALSA
                     GMILMVMVMGIAVIALIVYVERAQRRLLINYPKRQVGNRVYEGQTSFLPLKLNTAGVI
                     PPIFASSLLLLPTTITGFAAQGGPEWLQTLNAYLGHGRPLFMLLYAALIIFFAFFYTA
                     IVFNPVETADNLKKHGGFIPGIRPGEKTAQFIDHVLSRITVIGALYLAFVCLLPELLV
                     SQSGLPFYFGGTSILIVVSVTMDTVAQVHGYLLAHQYEGLIKRNKLKGKRR"
     gene            1874..2458
                     /locus_tag="PLQ11_07810"
     CDS             1874..2458
                     /locus_tag="PLQ11_07810"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HRK24844.1"
                     /translation="MRLILLGPPGAGKGTQAQRIVTKYGIPQLSTGDMLRAAVANKTP
                     IGLKAKAVMESGGLVSDEIVVGIVEDRIAEADAQKGFILDGFPRTVAQAEALDAMLTR
                     KGLKLNGCVELVVDETKLVDRIVRRAEEAKAAGQPVRKDDDPDVFKTRLAAYHRDTAA
                     VTPYYKAKGLLKTVDGMQPIDGVTASLMDVLSKL"
     gene            2694..3062
                     /gene="rpsM"
                     /locus_tag="PLQ11_07815"
     CDS             2694..3062
                     /gene="rpsM"
                     /locus_tag="PLQ11_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011651425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HRK24845.1"
                     /translation="MARIAGVNIPTQKRVVIALRYIHGIGPVNAVEICQKVGIPMDKR
                     VSQLSDAEVLQIREAIDRDYVVEGDLRRETAMNIKRLMDLGCYRGLRHRRGLPVRGQR
                     THTNARTRKGKPKAIAGKKK"
     gene            3123..3512
                     /gene="rpsK"
                     /locus_tag="PLQ11_07820"
     CDS             3123..3512
                     /gene="rpsK"
                     /locus_tag="PLQ11_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HRK24846.1"
                     /translation="MAKEATRVRRRERKNIASGVAHVNASFNNTMITITDAQGNTISW
                     SSAGALGFKGSKKSTPYAAQVAAEDAARKAAEHGMRTLEVEVTGPGSGRESALRALQA
                     AGFTVTSIRDVTPIAHNGCRPRKRRRV"
     gene            3589..4605
                     /locus_tag="PLQ11_07825"
     CDS             3589..4605
                     /locus_tag="PLQ11_07825"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HRK24847.1"
                     /translation="MIQKNWQELIKPNKLEVTPGDDPKRLATVMAEPLERGFGMTLGN
                     SLRRVLLSSLQGAAVTAIHIDGVLHEFSSIPGVREDVIDIVLNIKDVAIKMQADQPKR
                     LSLRKHGPGVVKAGDIAVTGDVQILNPEHVLCTLDEGAEIRMELTVATGKGYTPAERN
                     RPEDAPIGFIPVDSLFSPVKKVSYRVENTRSGQILDYDKLTMTIETDGSVSPEDALAF
                     AARILQDQLEIFLNFEEPKREEAPAAIPELAFNPALLKKVDELELSVRSANCLKNDNI
                     VYIGDLIQKTEGEMLRTPNFGRKSLNEIKEVLAQMGLHLGMEVTGWPPENIEELAKRF
                     EEHY"
     gene            4745..5185
                     /gene="rplQ"
                     /locus_tag="PLQ11_07830"
     CDS             4745..5185
                     /gene="rplQ"
                     /locus_tag="PLQ11_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HRK24848.1"
                     /translation="MRHGNRGRRFNRTASHRKAMFANLAQALITHEQIVTTLPKAKDL
                     RPVVEKLVTLGKRGDLHARRQAVAQVRDADVVKKLFDVLGPRYKARNGGYTRVLKAGF
                     RFGDSAPMAVIEFVDRDVDAKGKADLERHAATVAANAEGGNAEG"
     gene            5472..6134
                     /locus_tag="PLQ11_07835"
     CDS             5472..6134
                     /locus_tag="PLQ11_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24849.1"
                     /translation="MPDPAGDFSIFRRDVLTRLMYGSGTADAPASEDDRQQWFFTLIA
                     GAYRDIVRVEWGIDRFGISDQRLEEARLQWLRDIGRIEIDGSVEPDEYKLLGFLVYWL
                     RRRCVIGYAHRSEAACRVAEIDGWVNAQDQFLLRCNEIAAFLIGFRLCLYLRIGPNLT
                     TDANGHPIELLDSFDDHDLDQEFLFDIGTLMKQKNLSPHSMYIIFRSLLNSRPPPRPP
                     RH"
     gene            complement(6142..7071)
                     /locus_tag="PLQ11_07840"
     CDS             complement(6142..7071)
                     /locus_tag="PLQ11_07840"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRK24850.1"
                     /translation="MQATDLLHPVCQRLTGIVPSQNTPFTADGAVDAPAVRRLARASL
                     RAGVAGLLILAVASENRALTIDERARIGALFMEEIAGAIPVVVAVSAPDIDTAVILTR
                     QAVAIKADAVCYQAPAGLSRNALADQLARIADCGPRLIMLQDLDFNGPGLSVEDIAWL
                     YERQPLFRAIKVETQPAGPKYSDLLARFGGALHVSGGWAVMQMIEALERGVHAFMPTA
                     MDNIYVAIHRLFAAGRKDEARSLFERLLPFVAFSNQHIDVSIRFFKRLRLLDGTFSTD
                     HCRPPTPMLDPYQQATLNLLARRAVALDGEMGG"
     gene            complement(7131..7856)
                     /gene="phoU"
                     /locus_tag="PLQ11_07845"
     CDS             complement(7131..7856)
                     /gene="phoU"
                     /locus_tag="PLQ11_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HRK24851.1"
                     /translation="MVDHTVRAFDIELQDLARRITEMGGIAESMLGRAVEALVKGDLR
                     QAEEVIGTDAGLDELEREVNDKAILLIARRQPMAVDLRFIASTMKLAGDIERIGDLAK
                     NIGKRVKAIGVPFHHPSLVTGVTHLGDMAQDRLRQVLDAYARRDLDAAMEVWRHDDDI
                     DALHNSLFRELLTYMMEDPRNITFCTHLLFCAKNIERVGDHCTNIAEIMHYMLTGGAV
                     NQTRPRAGAVTEVDLAINGDTES"
     gene            complement(7872..8705)
                     /gene="pstB"
                     /locus_tag="PLQ11_07850"
     CDS             complement(7872..8705)
                     /gene="pstB"
                     /locus_tag="PLQ11_07850"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009450745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HRK24852.1"
                     /translation="MSLSSDPVGFRPPAAAHSSSGDSGAGATKVSARRCSVFYGAKQA
                     LFDVDVDIPERMVTAFIGPSGCGKTTFLRCINRMNDTIPIARVTGEIIIDGKNINSDK
                     VDPVALRARVGMVFQKPNPFPKTIFENIAYAPRIHGMATSRDDLAEIVEKSLRRAGLF
                     EEVKDRLDQPGTGLSGGQQQRLCIARAIAVNPEIILMDEPCSALDPIATAKIEELIDE
                     LRENYTIVIVTHSMQQAARVSQRTAFFHLGKLVEFGPTDRIFTAPNDQRTQDYITGRF
                     G"
     gene            complement(8726..>9072)
                     /locus_tag="PLQ11_07855"
     CDS             complement(8726..>9072)
                     /locus_tag="PLQ11_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006724603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate ABC transporter, permease protein
                     PstA"
                     /protein_id="HRK24853.1"
                     /translation="HILPYSTGGILTGVIIGMARAIGETAPIITIGALTFIAFLPTSP
                     ISPDFPFLSFQWLFDGFTVLPIQAFNWISRPGSDFHANAAAAGLVLLMITLAMNGFAI
                     YLRTRLRRKLRW"
BASE COUNT         1684 a         2702 c         2988 g         1698 t
ORIGIN      
        1 gcgtcgccat caagggcttc gagggcggcc agatgccgct gcatcggcgc ctgcccaagc
       61 gtggcttcac caacatcttc gcgctcgacc tcaacgaggt gaacatcggg cgcgtgcagc
      121 aggcggtgga cgccggcaag ctcgacgcca aggcgccggt gacggtggat gcgctggttg
      181 cggccggcgt ctgcgcccgc gcccgcgacg gggtgaagat cctcggcgtc ggcgagatca
      241 aggccaagct cagcttcgag gtgtatggcg cctccaagag cgcccgcgcc gcgatcgaga
      301 aggccggtgg cagcgtcaag ctgctgacgg agcagggcga agcgccgcag gcgtaatgga
      361 ctcatgccgt cgcggctggt gttccggccg cggcggacct atgtatgagc cggaaggttg
      421 cgtccggcag cgtccgggtc cccgcgttcc gtgccggttg gccggggcgg ggcgataaag
      481 cccggctgcg ggccgcaccg tcagggcaaa cagcgccggt ccgccggctg gcaggaacgg
      541 aacgcatggc ttctgcagca gaacaactcg catccaacct gaacttcggc gcgctggcca
      601 aggcggacga actgaagaag cgcatctggt tcacgctggg tgcgctcatc gtgttccgtc
      661 tgggcaccta cattccgctg ccggggatca atccggatgc cgtggcggag attttcaaga
      721 gccagcagaa cggcgtgctc gggctgttca acatgttctc gggcggcgcg gtcggccgtc
      781 tggcgatctt cgcgctcaac atcatgccgt atatctcggc ctcgatcatc atccagctga
      841 tgacgacggt cagcccccag cttgaggcgc tcaagaagga aggcgaggcc ggacggcggc
      901 agatcaacca gtatacgcgc tatctgaccg tggttctggc gctgtttcag gcttatggca
      961 tcgccatggg gcttgaagct tccggcaatg cggtgcgcga gccgggcctg ttcttccgcg
     1021 tctccaccgt tctgacgctg accggcggca cgctgttcat gatgtggctc ggcgagcaga
     1081 tcactgcgcg cggcatcggc aacggcacgt cgctgatcat cttcgccggc atcgtcgccg
     1141 aattgccggc ggcgatcgcc aatacgctgg aactgagccg tcagggcgcg ctttcggccg
     1201 gcatgatcct gatggtcatg gtgatgggca tcgcggtgat cgcgctgatc gtctatgtcg
     1261 agcgggcgca gcggcggctg ctgatcaact atcccaagcg acaggtcggc aatcgcgtct
     1321 acgaggggca gacctcattc ctgccgctca agctcaacac tgcgggcgtc attccgccga
     1381 tctttgcctc gtcgctgctg ttgctgccga ccacaatcac cgggtttgcg gcgcagggtg
     1441 gaccggaatg gctgcagacg ctcaatgcct atctcggcca cgggcggccg ctgttcatgc
     1501 tgctctatgc ggcgctgatc atcttcttcg ccttcttcta taccgcgatc gtgttcaatc
     1561 cggtcgagac ggccgacaac ctcaagaagc atggcggatt cattccgggc atccgccccg
     1621 gtgagaagac ggcgcagttc atcgatcatg tgctgtcgcg catcacggtg atcggcgcgc
     1681 tgtatctggc cttcgtctgc ctgttacccg aactgctagt ttcccagagc ggcctgccgt
     1741 tctactttgg cggcacctcc atcctgatcg tcgtctccgt gacgatggat acggttgcgc
     1801 aggtgcatgg ctatctgctg gcgcaccagt atgagggcct gatcaagagg aacaagctca
     1861 agggaaagcg tagatgaggc ttattctgct gggaccgccg ggggcgggca agggcacgca
     1921 ggcgcagcgt atcgttacca aatacggtat tccgcagctt tccaccggcg acatgctgcg
     1981 cgccgctgtg gccaacaaga ccccgattgg tctcaaggcc aaggcggtga tggaatcggg
     2041 cggcctggta tcggacgaga tcgtcgtcgg catcgtcgag gaccggattg ccgaggccga
     2101 tgcccagaag gggttcatcc tcgacgggtt cccgcgcacg gtcgcacagg ccgaggcgct
     2161 cgacgccatg ctgacccgca aggggctgaa gctcaacggc tgcgtcgaac tggttgtcga
     2221 cgagaccaaa cttgttgacc gaatcgtccg ccgcgccgaa gaggcgaagg ccgccggcca
     2281 gccggtgcgc aaggatgacg atccggatgt attcaagacc cgccttgcgg cctaccatcg
     2341 cgatactgcg gcggtgacgc cgtattacaa ggccaagggt ctgctcaaga cggtggacgg
     2401 gatgcagccg attgacgggg tgacagcctc gttgatggat gttctgtcaa agctttgagc
     2461 aaagacggat ttggggttga cgagcggcgc ctgcgcggct atcggctcca agttaccgat
     2521 gaagtgggcg ggcgcgcggt tttccgcgcg ctgctgcttt ggccggagcg gcgagggggg
     2581 cacccccgcg cgaccccggc tcgcacggca tcggttttca cattggccgg ggattgccct
     2641 tgccgttgga gcggtggctg ccaccgcgtg acattgcctt gaggagaagc gtcttggctc
     2701 gtatcgccgg cgtcaacatt ccgacccaga agcgcgtcgt gatcgcgctc cgctacattc
     2761 atggcatcgg cccggtgaac gcggtcgaga tctgccagaa ggtaggcatc ccgatggaca
     2821 agcgcgtcag ccagctgagc gacgccgaag tcctgcagat ccgcgaggcg atcgatcgcg
     2881 attatgtggt ggaaggcgac ctgcgtcgcg agaccgccat gaacatcaag cggctgatgg
     2941 atctgggctg ctatcgtggc ctgcggcatc gtcgcggcct gccggttcgc ggccagcgca
     3001 cgcacaccaa tgcgcgcacc cgcaagggca agccgaaggc catcgccggc aagaagaagt
     3061 aagtcgtttc gtgccggcgg catgccgccg gcgatcacct tagcagaaaa aggtttcagg
     3121 gcatggccaa ggaagcaacc cgcgtccggc gtcgcgaacg caagaacatc gcgtcgggcg
     3181 tggcgcatgt gaatgcgtcg ttcaacaaca cgatgatcac catcacggat gcgcagggca
     3241 atacgatctc ctggtcgtcg gctggcgctc tcggcttcaa gggctcgaag aagtcgacac
     3301 cttatgctgc gcaggtcgct gccgaggatg cggcgcgcaa ggccgccgag catggcatgc
     3361 gcactctgga agtcgaggtg acggggcctg gttcgggtcg ggaatcggcg ttgcgtgcgt
     3421 tgcaggcggc cggtttcacc gtgacgtcca tccgtgacgt gacgccgatc gcccataacg
     3481 gttgccggcc gcgcaagcgt cgccgcgttt gattttgtga tgcgtctcgg tgcggcgcgc
     3541 cttttccggc gcgccggttc tttacgcgag atttgaagga ccttcgtcgt gatccagaag
     3601 aactggcagg aactgatcaa gccgaacaag ctcgaggtga cgcccggcga cgatccgaag
     3661 cggctcgcca cggtgatggc ggaaccgctg gagcgcggct tcggcatgac gctcggcaat
     3721 tcgctgcggc gcgtgctgct ttcgtcgctg caaggcgcgg cggtgaccgc gatccacatc
     3781 gacggcgtgc tgcatgaatt ctcgtcgatc cccggcgtcc gcgaggacgt gatcgacatc
     3841 gtcctgaaca tcaaggacgt ggccatcaag atgcaggccg accagcccaa gcgtctcagc
     3901 ctgcgcaagc atggcccggg cgtggtcaag gccggcgaca ttgccgtgac cggcgatgtg
     3961 cagatcctca atccggagca tgtgctgtgc acgctggacg agggcgccga gatccgcatg
     4021 gaactgacgg ttgccaccgg caagggctat accccggccg agcgcaaccg gcccgaggat
     4081 gcgccgatcg gcttcattcc ggtcgacagc ctgttctccc cggtcaagaa ggtgtcctac
     4141 cgcgtcgaga acacccggtc tggccagatt ctcgactatg acaagctgac catgaccatc
     4201 gagaccgatg gatcggtgtc gccggaggat gcgctggcct ttgccgcccg catcctgcag
     4261 gatcagctcg agatcttcct caacttcgag gaacccaagc gcgaggaagc gccggctgcc
     4321 attccggagc tggccttcaa tccggcgctg ctcaagaagg tggacgaact cgaactgtcg
     4381 gtccgttcgg ccaactgcct gaagaacgac aacatcgtct acatcggcga ccttatccag
     4441 aagaccgagg gcgagatgct gcgcacgccg aacttcggcc gcaagtcgct caacgagatc
     4501 aaggaagtgc tggcgcagat gggtctgcat ctcggcatgg aagtgacggg gtggccgccg
     4561 gagaatatcg aagagctggc caagcgcttc gaggagcatt attgagtttt gcccgcgcct
     4621 ggcgacaggt gcgggatttt ctgtccgtag cgccagcccg gaaaccggga accacttttg
     4681 cggtgcacgg aataacgacc ggccgttcct tgacacggcg gccggataag gatggagcaa
     4741 tccaatgcgt cacggaaatc gcggtcgccg cttcaatcgc acggcctcgc atcgcaaggc
     4801 gatgttcgcc aatctcgccc aggcgctgat cacgcacgag cagatcgtca ccacgctgcc
     4861 gaaggccaag gacctgcgtc ccgtggtcga gaagctggtc acgctcggca agcgcggcga
     4921 tctgcatgcg cgccgtcagg cggtcgcgca ggtgcgcgac gccgatgtgg tcaagaaact
     4981 gttcgacgta ctcggcccgc gctacaaggc ccgcaacggt ggctataccc gcgtgctgaa
     5041 ggccggcttc cgtttcggcg attccgcccc gatggcggtg atcgagtttg tcgatcgcga
     5101 cgtcgatgcc aagggcaagg ccgacctcga acgccatgcg gcgacggtcg cggcgaatgc
     5161 cgagggcggg aacgccgagg gttaagtagc aattcaccgt cagtaggtta ggatgctatc
     5221 tgacggtcga gtcgcggggc gcacagatgg cacgcaggcc tttgacgttt gatccttcgc
     5281 ttgaagatgt ttttgtcggg gattccgatc cgcggctgtc gaagttatcg gtcgatgctc
     5341 gcattctgaa gtatggcaag aacgggccgt ccattcccac acgcgatctg actgaacgcg
     5401 aacgggcggc tatccggaaa attggctctc ctcgtcgtaa accctcctga ctttcagata
     5461 ttttcgtttc aatgcctgat cctgccggtg acttcagcat tttccgaaga gatgtcctga
     5521 cccgccttat gtatggcagc gggacagccg acgcgccggc atcggaagat gatcgtcagc
     5581 aatggttttt cacacttatc gctggcgcct atcgggacat tgtccgcgtc gaatggggca
     5641 tcgatcgttt cggaatttca gatcagcgcc tggaagaggc tcgtctacag tggctgcgtg
     5701 atatcgggcg catcgaaatt gacggcagcg tagagccgga cgagtacaag ctgctggggt
     5761 tcctcgtata ctggctcagg cgccgatgtg tgatcgggta tgcacaccgt tcggaggcag
     5821 cttgccgtgt ggcggagatt gatgggtggg tcaacgctca ggatcaattc ctgcttcgct
     5881 gcaacgagat tgctgcattt cttatcgggt tccgactgtg tctctacctt aggattggtc
     5941 ccaatcttac aacagacgcc aatggccacc cgattgaatt gttggattct tttgatgacc
     6001 acgatctcga tcaggagttc ctgttcgata tcggtacgtt gatgaagcaa aagaatctct
     6061 cgcctcactc gatgtatatt attttccgct ctctattgaa ttcccggcct ccgccgcggc
     6121 caccacgcca ctgactgtgg ttcatccccc catctcccca tccagcgcga ccgcccggcg
     6181 tgccagcagg ttcagagttg cctgctgata cgggtccagc atcggggttg gcgggcggca
     6241 atggtcggtt gaaaaggttc cgtccagtag tctgagccgc ttgaaaaagc ggatcgagac
     6301 gtcgatgtgc tggttggaga aggcgacgaa cggcagcaac cgctcgaaca atgagcgggc
     6361 ttcgtccttg cggcctgcgg caaacagccg gtggatggcg acatagatat tgtccatcgc
     6421 cgttggcatg aaggcatgga cgccgcgctc cagcgcctcg atcatttgca tcactgccca
     6481 gccgcccgaa acatgcagtg cgccgccgaa gcgggcgaga aggtcgctgt atttcggtcc
     6541 ggcgggctgg gtttccacct tgatggcgcg aaacagcggc tggcgttcgt agagccaggc
     6601 gatgtcctcg acggacaggc cggggccgtt gaaatcgaga tcctgcagca tgatcagccg
     6661 cgggccgcaa tcggcgatgc gggcaagctg atcagccagc gcgttgcggg aaaggccggc
     6721 cggggcctgg tagcaaaccg catcggcctt gatggcgacc gcctggcggg tcaggatcac
     6781 ggcggtatcg atgtccgggg cagagacggc gaccacaacc gggatggccc cggcgatctc
     6841 ctccatgaac agggcgccga tgcgggcgcg ctcgtcgatc gtaagcgcac ggttctcgct
     6901 ggcgactgca agaatcagca ggccggcgac gccggcgcgc agcgaggccc gggcaagacg
     6961 gcggacagcc ggagcgtcga cggcgccatc ggctgtgaac ggagtattct gacttggcac
     7021 aatcccggtc agccgctggc agacagggtg gagcaggtcg gttgcttgca tgagtcttcc
     7081 cagtgcagat caggacggag tttggccgcc cctcaggccg ctggcaaggc tcaggactct
     7141 gtatcgccgt tgatggccag atcgacctcg gtcacggccc cggcgcgggg gcgtgtctgg
     7201 ttcaccgcgc cgccggtcag catgtaatgc atgatctcgg cgatattggt gcaatggtcg
     7261 ccgacccgct cgatgttctt ggcgcagaac aagaggtggg tgcagaaggt gatgttgcgc
     7321 ggatcttcca tcatgtaggt cagcaattcg cggaacagcg aattatgcag cgcgtcgata
     7381 tcgtcgtcgt ggcgccaaac ttccatggcg gcgtcgaggt cacggcgggc ataggcgtcg
     7441 agcacctggc gcaggcggtc ctgcgccatg tcgccgagat gggtcactcc ggtgaccagc
     7501 gaggggtggt gaaacggcac gccgatggct ttcacccgct tgccgatgtt cttggccaga
     7561 tccccgatcc gctcgatatc gccggccagt ttcatggtgg aggcgatgaa ccgcagatcg
     7621 accgccatcg gctgccggcg ggcaatcagc aggatggcct tgtcattgac ctcgcgttca
     7681 agctcgtcga gccccgcgtc cgtgccgata acctcttcgg cctggcgcag gtcacccttg
     7741 accagcgcct cgacggcgcg gcccagcatg ctttcggcga tgccgcccat ctcggtgatc
     7801 cggcgggcaa gatcctgcaa ttcgatgtcg aaggcgcgga cggtatggtc aaccatggaa
     7861 cagacctcag atcagccgaa gcggccggtg atgtaatcct gtgtgcgctg atcattcggc
     7921 gcggtgaaga tgcgatcggt cggcccgaat tcgacgagtt tgccgagatg gaagaaggcg
     7981 gttcgctggg agacacgcgc cgcctgctgc atcgaatggg tgacgatgac gatcgtgtag
     8041 ttctcgcgca attcgtcgat caactcctcg atcttggcgg ttgcgatcgg atcgagcgcc
     8101 gaacacggct catccatcag gatgatttcc ggattgaccg caatggcgcg ggcaatgcac
     8161 aggcgctgct gctgacctcc ggagagcccg gtgccgggct ggtcgaggcg atccttgacc
     8221 tcttcgaaca ggccggcgcg gcgcaggctt ttctcgacga tttcagccag atcatcgcgg
     8281 ctggtcgcca tgccgtgaat gcgcggggca taggcgatgt tctcgaaaat ggtcttggga
     8341 aacgggttgg gtttctggaa caccatgccg acgcgggcac gcaaggcgac cggatcgacc
     8401 ttgtcggaat tgatgttctt gccgtcgatg atgatctcgc cggtcacccg cgcgatcggg
     8461 atcgtgtcgt tcatgcggtt gatgcagcgc aggaaggtgg tctttccgca gccggagggg
     8521 ccgatgaacg ccgtcaccat gcgctcggga atatcgacat cgacatcaaa cagggcctgc
     8581 ttggcgccat agaaaacgct gcacctgcgg gcggagacct tggtcgcacc cgcgccgctg
     8641 tcgccgctgc tggaatgggc ggcggcgggc gggcgaaagc cgacgggatc ggaggacagg
     8701 gacatgatct tttactccgt catgatcacc agcgcagctt gcggcgcaag cgcgtgcgca
     8761 gatagatggc aaaaccgttc atcgccagcg taatcatcag cagcaccaga ccagccgccg
     8821 cagcgttggc atgaaagtcc gaacccgggc gcgaaatcca gttgaaggcc tggatcggca
     8881 gcacggtgaa cccgtcaaac agccactgga agctcaggaa gggaaagtcc ggcgaaatcg
     8941 gcgaggtcgg caggaaggcg atgaaggtca gcgcgccgat ggtgatgatc ggcgccgtct
     9001 cgccgatggc gcgcgccatg ccgatgatga cgccggtgag gatgccgccg gtcgaatacg
     9061 gcaggatgtg cc
//
LOCUS       DAOMXG010000099         9052 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_4064_length_9052_cov_10.021785, whole genome shotgun sequence.
ACCESSION   DAOMXG010000099 DAOMXG010000000
VERSION     DAOMXG010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 9052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9052
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4064_length_9052_cov_10.021785"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..934)
                     /locus_tag="PLQ11_07860"
     CDS             complement(17..934)
                     /locus_tag="PLQ11_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRK24854.1"
                     /translation="MLSLVRRLTGLVPVLVAATLVVFLFLRLLPGDPARLIAGSEASA
                     ADVAALRADMGLDRPIWQQYGSFLMRAVQGDFGLSLRTRRPVVEEVRARLSSSLLLAT
                     AAAGWAVVAGVALGAWTVAHRGGRIDLAGRLVAVAGVAMPSFWLGLLLIDVFAVRLGW
                     LPTGGGGSFSHLVLPAIALGTAMAATLARVTRAAMIEVLGEDFVRTARAKGLGEPIVL
                     WRHVLRVALAPVVTLAGVQFGSLLGGAVVVETVFAWPGLGRLLAESVAMRDYPVIQAI
                     LLVYVLCFALVNLCVDLAYGMIDPKVRTS"
     gene            complement(957..2480)
                     /locus_tag="PLQ11_07865"
     CDS             complement(957..2480)
                     /locus_tag="PLQ11_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009762644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein"
                     /protein_id="HRK24855.1"
                     /translation="MRSLVAMIVAGLVLLWQPPVRAAGNVVIGVADTLTGFDVHTISD
                     TLSQSAARMMLQGLFGFDSDLKPVPVLAESYEANSEATEFTFYLRKGVRFHDGTPFNA
                     EAVRINFERLVNPENRLSRASLLSMARSFTVVDEFTLRISLKTPFAALINTLAHPGTM
                     IHSPAALARHGRDVMRHPVGTGPFRFAEWQADRLKVVRNEHYWKPGLPKVDSITLRSV
                     PENGSRLAMLLAGEAHAIAPLPPEQVSVAEANPAIDVVRRPSIVTWYMAMNTRKAPFN
                     DVRVRQALNLAVDKQAYRQVVWNGFAAPLTAPVPPGLPMHAEQQPWPYDPERARALLG
                     QAGYPNGFSVELMGAGNTLARRAMQFLQQQLAAVGVQVRITPLDAGMIAERIWRVADP
                     AAATIELFYAGWSASTGDPDWQLRPLFFGPSAPPALNNVAYYSNPAVDAAIMAGLSTI
                     DPQRRAEAYRVAQEIIWTEAPWVFLGVETLLAGKAKSVAGLHYLPDRSLSLDEVELK"
     gene            complement(2850..3536)
                     /locus_tag="PLQ11_07870"
     CDS             complement(2850..3536)
                     /locus_tag="PLQ11_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018097879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRK24856.1"
                     /translation="MAAILAAEGLTRRLSGDLPVTLVDGVDLSIEEGGLVAITGPSGS
                     GKSSLLYLLGLLDAPTAGDVRLAGQRTATLDDEARARLRLTMLGFVFQFHFLLPEFSA
                     LENVMLPMRALKAMPETRMRVRAAEILESLGLADHIGKTPDKLSGGQRQRVAIARALA
                     NRPKVLLADEPTGNLDSASTAQVLAIFRELVAEHAATIIMVTHDPGLAAAAGRRIHVI
                     DGRLAEPPSG"
     gene            complement(3536..4771)
                     /locus_tag="PLQ11_07875"
     CDS             complement(3536..4771)
                     /locus_tag="PLQ11_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRK24857.1"
                     /translation="MNLVVHIAWTHIRHRLRQTLVGTLGIATGVGFSIMMASLMVGSQ
                     RDFIATLVDTLPHVSIRDEQRHAQRQPAENAYDVAAVSGVRPDENRPGLKNPLALVNA
                     LEGWVPGAVATSVTTRAIVRYAGRDLAATVLGIDPRREPRVSSLPKQMRQGTLAALNA
                     VPNGIIIGDGLMNKLGARQGATLALVPATGVPVDARIAGVFHSGVRAVDEGQIYTLTR
                     TAQILAGQTGLINEIRIRLDDAMQSRDVAALVEAQVGYKALSWQEANEDLMSAFMVRN
                     IIMFTVVGAILLVASFGTYNIVSTITHEKTRDIAIMKALGLPRATVRRIFVLEALLIG
                     VFGAVLGCVLGNLMTQALGQVEIKVPFGDATTLPVIYDGMHYLGASLVAMASSLIAGF
                     LPARKAAALHPVDIIRGAT"
     gene            complement(4768..5778)
                     /locus_tag="PLQ11_07880"
     CDS             complement(4768..5778)
                     /locus_tag="PLQ11_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRK24858.1"
                     /translation="MKWIKRLVLLLMLAAGGGGAWLWLQRGQLVVVQEVRRGTAAEIV
                     YGTGVVEPVEWAKILAPVRARIEVHCRCEGRSVKAGDVLARLDEKAPRAELAQLEARK
                     TFLTNEMTRQADLIARGTVSRQTYERAQSEFGQVEAQIAAATARLGDYTIVAPIDGVV
                     LRADGQVGEIASTAEPLFWVGKPRPLHVITDVNEEDIARVREGQVVLLRSDAYADRAL
                     TATVASITPKGDPVRKTFRLYLGLGDDTPLRIGMSVEANIVTREKADVLVVPAEALRG
                     SQVFVLEGEHARQRPVVIGIRGTRQIEIVEGLAAAEKVIAPVPAKLADGAKVRLIERT
                     GP"
     gene            5979..6920
                     /locus_tag="PLQ11_07885"
     CDS             5979..6920
                     /locus_tag="PLQ11_07885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRK24859.1"
                     /translation="MRLTARLLLAATAIATSLGIAAAQDLRMMTGPQGGIWVPLGGQL
                     KDLWEKAIPGARFSTLPGAGIANARAIEESKADVGFGNSITTADAIAGNAPFEKKHGK
                     ICNVASLYPQFFQAIVLADSGINSIKDLKGKTITTQQRGNTGELITQNFLKAYGMSYS
                     DVKVNFGSYTESVEQMKDKQAQLFTLGAGAPLGAVMDLASARDIRLLDQTEAIDAMRQ
                     INPGYVLLPIAKGTYPKQDKDVNVIAYATHVIVSCALPSDTVYQMTKAIADNTALLGN
                     LNKDMGRLSPKEMAASVGVPFHPGAARFYAEKGIALQ"
     gene            7025..8914
                     /locus_tag="PLQ11_07890"
     CDS             7025..8914
                     /locus_tag="PLQ11_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HRK24860.1"
                     /translation="MSLSWGKASGTMRLIAVLAITMSLYHMWVVWFGAPEQHLFRALH
                     LAFALTLTLLWYRRSTLTEEELAMRTDGAPAFIGVDAADLALIGLGLVALGHVWVNYS
                     YFVNRIYYVDDLSLFDQLAAIVLIGLVLEASRRLMGWALPLTALSFLAYGLTIARVEP
                     MRLLDQLYMTTEGIFGMPLGVSASYVMIFVLFGAFMERTGTGQLFMDFAMALTGHTAG
                     GPGKVSVVSSSLFGTISGSAVANVMVDGPISIPLMKRTGFKPHFAAAVEAVASTGGQI
                     MPPIMGAAAFVMAEFMGVSYGQVALWATIPAILYYVACFASVHFEAKRQQLHGVPRAE
                     LPRLGPTLVERGHLFLPVLTILAVMYAGYSAPLAALIGLLACFPVAALRASTRANVTV
                     QNVIEALVDGARNTLAVALACACAGIIIGIVTLTGLGITFTQFVVGLSKDTLLLALIL
                     TMLAGIVLGMGMPTTPAYIIMSALLIPAIIKLGVPKPAAHMFAFYFAILSAITPPVAL
                     AVYAAAGLAKSDIWRTGWAAVKVGSAGFIVPFMFVYEPALLMIGDWNTILSATITATA
                     GCLLLAAGLAGYWIAPASQLQRLLLVSAALALVTPGLITDLIGAGLAALVTLWQLAGR
                     GRRPN"
BASE COUNT         1669 a         3065 c         2743 g         1575 t
ORIGIN      
        1 aacggcgctg gccccgtcat gacgtgcgca ctttcggatc gatcattccg taggccaggt
       61 caacgcacag gtttaccagc gcgaagcaca gcacatagac cagcaggatc gcctgaatga
      121 ccgggtaatc gcgcatggcc acagactcgg ccagcaaccg cccgagcccc ggccaggcga
      181 acaccgtttc caccaccaca gccccgccga gcaggctgcc gaactgcaca ccggccagcg
      241 tcaccaccgg agccagcgcc acgcgcagca catgccgcca cagcacgatc ggctcgccga
      301 gacccttggc gcgggcggtg cggacgaaat cctcgcccag cacctcgatc atcgccgcac
      361 gggtgacgcg cgccagagtg gccgccatcg ctgttcccag ggcgatggcc ggcagcacga
      421 gatgggagaa cgaaccccct cctccggtcg gcagccagcc gagccgcacg gcgaacacat
      481 cgatcagcag cagcccgagc cagaacgagg gcatcgccac tccggcgacg gcgaccagtc
      541 gcccggccag atctatcctc cctccacgat gcgccaccgt ccaggcgccg agcgccaccc
      601 cggccacaac cgcccacccg gcagcagccg tcgccaacag caggctggag gacagccgcg
      661 cccgcacttc ttcgacaacc gggcggcgcg tgcgcaacga caggccgaaa tccccctgca
      721 ccgcccgcat gaggaaggag ccatattgct gccagatcgg ccgatccagc cccatgtcgg
      781 cgcgcaatgc cgcgacatcc gctgcgctgg cctcggatcc ggcgatcaac cgggccggat
      841 cgccgggcaa cagccgcagg aacaggaaaa ccacaagtgt cgccgcgaca agcaccggca
      901 ccagcccggt cagccgccgc accaaactca gcatggccgc cccggagaca ctccggtcat
      961 ttcagctcca cctcgtccag cgacaggctg cggtccggca gataatgcag ccctgcaaca
     1021 gactttgcct tgccggccag cagggtctca accccgagaa agacccaggg tgcctcggtc
     1081 cagatgattt cctgcgccac ccgataggct tcggccctgc gctgcggatc gatggtcgac
     1141 aatccggcca tgatcgccgc atcgacggcg ggatttgaat aataggcaac gttgttgagg
     1201 gccggcgggg cactcggccc gaagaacagc ggccgcaact gccaatcggg atcaccggtc
     1261 gaggccgacc agccggcata gaacagctcg atcgtcgctg cggcaggatc cgccacccgc
     1321 cagatgcgct cggcaatcat gccggcatcg agcggagtga tccgcacctg cacccccact
     1381 gccgccagct gctgctgcag aaactgcatg gcgcggcggg cgagcgtatt gccggcgccc
     1441 atcagttcga cagaaaaccc attcgggtac cctgcttggc cgagcaaggc ccgcgccctc
     1501 tccggatcat acggccaggg ctgctgctcg gcatgcatcg gcagccccgg cggaaccggc
     1561 gcggtgagcg gcgcggcaaa cccgttccat accacctgtc tgtaagcctg cttgtcgaca
     1621 gcaagattca gtgcctggcg cacgcgcaca tcgttgaacg gcgccttgcg cgtattcatt
     1681 gccatatacc aggtgacgat cgacggccgc cgcaccacat cgatcgccgg attggcttcg
     1741 gcgacgctca cctgctcggg cggcagcggc gcgatggcat gcgcctcccc tgccagcagc
     1801 atcgccagcc tcgacccgtt ttccggaacc gaccgcaggg tgatactgtc caccttcggc
     1861 aggcccggct tccagtagtg ttcgttccgc accactttca gccgatccgc ctgccattcg
     1921 gcaaaccgga acggtccggt cccgaccgga tgacgcatca catcgcgccc atgtcgggcg
     1981 agcgccgctg gactgtgaat catcgttccg ggatgggcca gtgtattgat cagcgccgca
     2041 aacggcgtct ttaggctgat cctgagcgtg aattcgtcaa caaccgtgaa cgaccgcgcc
     2101 attgacagca gactggctcg tgacaaccgg ttctcgggat tgaccagccg ctcgaaattg
     2161 atccggaccg cctcggcatt gaacggcgtg ccgtcatgaa agcggacacc cttgcgcaga
     2221 tagaaggtga attcggtcgc ctcggaattg gcctcgtagg attccgccaa aaccggcacc
     2281 ggcttgaggt cgctatcgaa gccgaacagc ccctgcagca tcatccgtgc agctgactga
     2341 gacagggtat cggagatcgt gtgaacgtcg aagccggtca acgtgtcggc aacgccgatc
     2401 accacattgc cggcggcccg caccggcggc tgccacagca gcacaaggcc agcgacaatc
     2461 atcgctacaa gcgagcgcat caattgcttc tcccatcgct caggtcagca actgcggcaa
     2521 tcgacaacag ccacagatcc gaaaccccaa ggagcaccag cgactgacgt gcctgccacg
     2581 gcgggtccga cctcgccctg gatcggcccg gcagtcctga ccggcaagat tgcgaacatc
     2641 aagccagggt tttccctcga ctcttttttg tgctggcaac aggccatggg cgcgcaccaa
     2701 cgtcaagcgc tcattgcaaa aatgctactt aaataggaga aaaacaatat aaaaaatccc
     2761 aaaatataag aaaattacat caaaatgatc ggtattcttg tgcctctcac cccaaaccgc
     2821 ctttcgtcat tgcgattctg cagtcatgat catccggatg gaggctcggc caaccgcccg
     2881 tcgatgacat gaatgcgtcg cccggcagcg gctgccagcc caggatcatg ggtgaccatg
     2941 atgatcgtgg ccgcatgttc ggcgaccagt tcgcgaaaga tcgccagcac ctgcgccgtg
     3001 gaggcactgt cgagattgcc ggtgggttca tcggccagca acactttcgg ccggttggcg
     3061 agcgcgcggg caatggcgac ccgctggcgc tgtccccccg aaagcttgtc cggcgtcttg
     3121 ccgatatggt cggccaggcc aaggctttcg aggatctcgg cggcacggac ccgcatgcgg
     3181 gtttctggca ttgccttcag cgcccgcatg ggaagcatga cgttttccag cgccgagaat
     3241 tccggcagga ggaaatggaa ctggaacacg aagccgagca tcgtcagccg cagccgggcg
     3301 cgcgcttcat catccagcgt ggcggtgcgc tgtccggcaa gccgcacgtc gccggcggtc
     3361 ggcgcatcca gcagcccgag gagatacagc aatgaggatt tgcctgatcc tgacgggccg
     3421 gtgatggcca caaggccgcc ttcctcaatg gacagatcga ccccgtcgac cagtgtgacc
     3481 ggcaaatcgc ccgaaagcct gcgggtcagt ccctcggcgg caaggatcgc tgccatcagg
     3541 tcgccccgcg gatgatatcg accggatgca acgccgctgc cttgcgcgcc ggcaggaacc
     3601 cggcgatcag cgaagaggcc atcgccacca ggctcgcgcc gaggtagtgc atcccgtcat
     3661 agatgaccgg cagcgtggtc gcatcgccga acggcacctt gatctcgacc tggccgagcg
     3721 cctgggtcat caggttgcca aggacacagc cgagcacggc gccgaacacg ccgatcagca
     3781 gcgcttccag cacaaagatg cgccgcaccg ttgccctcgg caggccgagc gccttcatga
     3841 tggcgatgtc acgcgtcttt tcgtgggtga tggtcgaaac gatgttgtag gtgccgaagc
     3901 tggcaaccag caggatcgcg ccgaccacgg tgaacatgat gatgttgcgc accatgaagg
     3961 cactcatcag atcctcgttg gcctcctgcc aggacagcgc cttgtagccg acctgcgcct
     4021 cgaccagcgc cgcgacatcg cgtgattgca tcgcatcgtc gagccggatg cggatctcgt
     4081 tgatcagccc tgtctggccg gcgagaatct gggccgtgcg ggtcagtgtg tagatctgcc
     4141 cctcatcgac cgcccgaacc ccggaatgga acaccccggc gatccgcgca tcgaccggca
     4201 ctcccgtcgc cggcaccagc gcaagcgtgg cgccctgccg tgccccgagc ttgttcatca
     4261 gcccgtcgcc gatgatgatg ccgttcggaa ccgcattgag cgcggccagc gttccctggc
     4321 gcatctgctt gggcagcgac gagacacgcg gctcgcgccg cggatcgatg ccgagcacgg
     4381 tggcggcaag atcgcgcccc gcataacgga cgatggcgcg tgtcgtcacc gaggtcgcca
     4441 ccgcccccgg cacccagcct tccagcgcat tgaccagcgc cagcgggttt ttcaggccgg
     4501 gacggttttc gtcaggccga accccgctga ccgcagcgac atcataggcg ttctcggccg
     4561 gctggcgttg tgcatgccgt tgctcgtcac ggatcgacac gtgcggcaag gtgtcgacca
     4621 gtgtggcgat gaaatcccgc tgcgagccga ccatcagcga cgccatcatg atcgagaagc
     4681 cgaccccggt ggcaatgccg agcgtgccga ccagtgtctg gcgcagccga tgccggatat
     4741 gggtccaggc gatgtggacg acgagattca tggtcctgtc cgctcgatca ggcgcacctt
     4801 ggccccgtcg gccagtttcg ccggcaccgg agcgatgacc ttttcggcgg cggcaagccc
     4861 ctcgacaatc tcgatctgcc gcgtgccgcg aataccgatg accaccggcc gctggcgggc
     4921 atgctcgccc tccagcacga agacctgcga gcctcgcaac gcctctgccg gaaccaccag
     4981 cacatcggcc ttttcgcgcg tgacgatatt ggcctcgacg ctcatgccga tccggagcgg
     5041 cgtatcgtcc ccaagcccga gatagagacg gaaggtcttg cgcaccggat cgcccttcgg
     5101 agtgatcgag gcgacggtcg cggtcagcgc gcggtccgca taggcatcgg atcgcagcaa
     5161 cacgacctgc ccttccctta cgcgggcgat atcctcttca ttgacatcgg tgatgacatg
     5221 cagcgggcgc ggcttgccca cccagaacag cggctcggcc gtgctggcga tctcacccac
     5281 ctgcccgtcg gccctgagca cgacgccgtc gatcggcgca acgatggtgt aatcgcccag
     5341 ccgggccgtt gcggcggcga tctgcgcctc cacctgaccg aactcgctct gcgcccgctc
     5401 gtaggtctgg cgcgagaccg tgccgcgcgc aatgaggtcg gcctgccggg tcatctcgtt
     5461 ggtcaggaat gtcttgcgcg cttccagttg cgccagttcg gcccgcggtg ccttctcatc
     5521 gagccgggcc agcacatcgc cggccttcac gctgcggccc tcgcagcggc agtggacctc
     5581 gatccgcgcc cgcaccggcg ccagaatctt cgcccattcg accggttcga ccaccccggt
     5641 gccgtagacg atctcggcag ccgtgccgcg ccggacctcc tgcaccacca ccagctggcc
     5701 gcgctgcaac cacagccacg cgccaccgcc cccggctgcc agcatcagca gcagcaccag
     5761 gcgcttgatc cacttcatgg gggcgctcct tgcgctggca ttgcatgagt cgctcctgat
     5821 gtaagcgttc ggcgcaacaa gaacaacagc cgggcagaca ctcggaaggg cttgcatggg
     5881 cgcctcattg cgctagcttg tcttacaatt gaccccgaaa acccgggcag gaaaccagca
     5941 cggagaaggc cggggcagtc tatcgaggag gatcgtccat gcgtctgact gcacgcctct
     6001 tgctggctgc aaccgccatt gccacgtcgt tgggcattgc agctgcccag gacctcagga
     6061 tgatgaccgg cccgcagggc ggcatctggg tgccgctcgg cggccagctc aaggatttgt
     6121 gggaaaaggc cattcccggc gccaggttct ccaccctgcc cggcgccggc attgccaatg
     6181 cccgcgccat cgaggagagc aaggccgatg tcggcttcgg caattcgatc accacggcgg
     6241 atgcgattgc cggcaatgca ccgttcgaaa agaaacacgg caagatctgc aacgtcgctt
     6301 cgctctatcc gcagttcttc caggcgattg tccttgccga cagcggcatc aactcgatca
     6361 aggacctcaa gggcaagacc atcaccaccc agcagcgcgg caacaccggc gaactgatca
     6421 cccagaactt tctcaaggcc tatggcatgt cctacagcga cgtgaaggtg aacttcggtt
     6481 cctacaccga gagcgtcgag cagatgaagg acaagcaggc gcagctgttc acccttggcg
     6541 ccggcgcgcc gctgggcgcg gtgatggacc tggcctcggc gcgtgacatc cgcctgcttg
     6601 accagaccga ggccattgat gcgatgcggc agatcaaccc cggctatgtg ctgctgccca
     6661 tcgccaaggg cacctacccc aagcaggaca aggacgtgaa cgtgatcgct tatgcgaccc
     6721 atgtcatcgt ctcctgcgcc ctgccaagcg acaccgtcta tcagatgacc aaggcaatcg
     6781 ccgacaacac ggccctgctc ggcaacctca acaaggacat ggggcggctg agcccgaagg
     6841 aaatggccgc cagtgtcggc gttccgttcc atcccggcgc tgcacgattc tacgcggaaa
     6901 agggtatcgc gctccagtag gcgtcgcgtc cctgcacgaa tcaggccggc ccggacacac
     6961 ctggttcgcg gctggtccac acgggccggg tgcggccttg gcggccaacg agggaggagt
     7021 gaaggtgagc ctgtcctggg gcaaggcatc aggcaccatg cgcctcatag ccgtgctggc
     7081 gatcaccatg tcgctgtatc atatgtgggt ggtctggttc ggagccccgg aacagcatct
     7141 tttccgcgcc ctgcacctgg cgttcgccct gacgctgacc ttgctgtggt atcgccgctc
     7201 gaccctgacc gaggaagagc tggcgatgcg cacggacggc gctcccgcct tcatcggcgt
     7261 ggatgcggcg gacctggcac tcatcggcct ggggctggtc gcgcttggcc atgtctgggt
     7321 caattattcc tacttcgtca accgcatcta ttatgtcgat gacctgagcc tttttgacca
     7381 gctggcggcg atcgtgctga tcggcctggt gctggaggcc tcgcgccggc tgatgggctg
     7441 ggcactgccg ctgacggcac tttcgtttct cgcctatggc ctgacgatcg ccagagtcga
     7501 gccgatgcgc ctgcttgacc agctctacat gaccaccgag ggcattttcg gcatgcccct
     7561 cggcgtttca gcctcttatg tgatgatttt cgtcctgttc ggcgccttca tggagcgcac
     7621 cgggacaggt cagctgttca tggactttgc catggcgctg accggccata ctgcgggcgg
     7681 gccgggcaag gtctcggtgg tctcgtcctc gctgttcgga acgatttccg gttccgccgt
     7741 cgccaatgtg atggtcgacg gtccgatttc cattccactg atgaagcgca ccgggttcaa
     7801 gccgcatttc gccgccgccg tcgaggccgt tgcgtccacc ggcgggcaga tcatgccgcc
     7861 gatcatgggg gctgccgcct tcgtgatggc tgagttcatg ggggtaagct acggccaggt
     7921 ggcgttgtgg gcgacgattc cggccatcct ctattacgtc gcctgcttcg cctcggtgca
     7981 ttttgaagcc aagcggcagc agctgcatgg tgtgccccgc gccgaactgc cccggcttgg
     8041 cccaaccctt gttgagcgcg gccatctgtt cctgccggtc ctgaccattc ttgctgtgat
     8101 gtatgccggt tacagcgcgc cgctggcggc gctgatcggt ctcctggcct gctttccggt
     8161 tgcggcgctc cgggcttcca cgcgcgccaa tgttacggtt cagaacgtca tcgaggcgct
     8221 cgtcgacggg gcgaggaaca cgctcgccgt tgcgcttgcc tgcgcctgtg ccggcatcat
     8281 catcggcatc gtgacgctca ccggcctcgg catcaccttc acccagttcg tggttggcct
     8341 gtcgaaagat acactgctgc tggcgctgat cctgacgatg ctggccggca tcgtgctcgg
     8401 catgggcatg ccgacgacgc cggcctatat catcatgtcg gccctgctga tcccggcgat
     8461 catcaagctt ggcgtgccca agcctgcggc gcatatgttc gccttctatt ttgccattct
     8521 ttcggccatc acaccgcctg ttgcgctggc tgtctatgct gcggcaggac tcgccaagtc
     8581 ggacatctgg agaaccggct gggcggcggt caaggtcggc tcggcgggat tcatcgtgcc
     8641 cttcatgttc gtctacgaac cggcactgct gatgatcggc gactggaaca ccatcctcag
     8701 cgccacgatc accgcaacag ccggctgcct gttgctggcg gcagggcttg ccggctactg
     8761 gatcgcaccg gccagccaac tccagcgcct gttgctggtc agcgctgctc tggcgctggt
     8821 gacacccggc ctcatcaccg atctgatcgg cgccgggctc gctgcgctgg tcaccctctg
     8881 gcagctggcc ggacgcggca ggcggccaaa ttagagcatt ttcaagcgaa gtggattccg
     8941 gttcgcgtca agaaaatggg aaaaaacaga aagatagagc attattgtga attggagttc
     9001 actgaaagtg ctctaaggcg cgtggggatt catcgtgaat tgatgacggc tg
//
LOCUS       DAOMXG010000100         8935 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_4138_length_8935_cov_10.497185, whole genome shotgun sequence.
ACCESSION   DAOMXG010000100 DAOMXG010000000
VERSION     DAOMXG010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 8935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8935
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4138_length_8935_cov_10.497185"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..1505)
                     /gene="ahcY"
                     /locus_tag="PLQ11_07895"
     CDS             complement(114..1505)
                     /gene="ahcY"
                     /locus_tag="PLQ11_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010685907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HRK24861.1"
                     /translation="MDYIVRDIGLADFGRKEIAIAETEMPGLMAIRKEYGTRQPLKGA
                     RIAGSLHMTIQTAVLIETLKALGADIRWASCNIYSTQDHAAAAIAAGGIPVFAVKGET
                     LTEYWDYTRKILEWGDGGTPNMILDDGGDATLLVHLGLRAEQGDTAFLDKPGSEEEEI
                     LFALIKRTLKEKPGWFAALAKSIKGVSEETTTGVHRLYIMAKEGKLLFPAINVNDSVT
                     KSKFDNLYGCRESLVDGIRRGTDVMMAGKVAMVAGFGDVGKGSAASLRQAGCRVLVSE
                     IDPICALQAAMEGYEVTTMEDAVGRADIFVTATGNKDVITIDHMRGMKDRAIVCNIGH
                     FDNEIQVAGLKNYKWHNVKPQVDEIEFPTGRRIILLSEGRLVNLGNAMGHPSFVMSAS
                     FSNQVLAQIELFCNPGKYDKTVYTLPKHLDEKVAALHLEKIGVKLTRLRPDQAAYIGV
                     SEQGPFKPDHYRY"
     gene            complement(1710..2024)
                     /locus_tag="PLQ11_07900"
     CDS             complement(1710..2024)
                     /locus_tag="PLQ11_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015821861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HRK24862.1"
                     /translation="MTETEPCPDDRFPEGAIIKVMPIINRKGLHARASAKFVQCADRF
                     ASAVTVHRCGETVGGTSIMGLLTLAAAQGTSITVAAVGEDARQAIDALAQLIADRFGE
                     DE"
     gene            complement(2021..2422)
                     /locus_tag="PLQ11_07905"
     CDS             complement(2021..2422)
                     /locus_tag="PLQ11_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003606016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRK24863.1"
                     /translation="MIGLVLVSHGRLALEFKAAMEHVVGQQRQVATICIGPDDDMEMR
                     RKEIVEAIARSDSGHGVVVLTDMFGGTPSNLAISAMNGDRVEVVAGINLPMLVKLASL
                     RECNDLDQAVTGAQEAGRKYINIASKVLQGK"
     gene            complement(2517..2987)
                     /locus_tag="PLQ11_07910"
     CDS             complement(2517..2987)
                     /locus_tag="PLQ11_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24864.1"
                     /translation="MSTDRYSHATCVLIGEQGVLIRGVSGSGKSRLAELLLRAEAAAR
                     RFACLVGDDRLRLVARHGRLLATVHPAIAGRRELRGLGLVAGNYEPTARLSLVIELGA
                     TPAERMPPSDLHVRLEGIALPLLRLSDGDIGPFAVALVQERLQQLDRNTMFRPA"
     gene            complement(2984..4681)
                     /locus_tag="PLQ11_07915"
     CDS             complement(2984..4681)
                     /locus_tag="PLQ11_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stimulus-sensing domain-containing protein"
                     /protein_id="HRK24865.1"
                     /translation="MRRRWIRRLWQPIWKPLTSTVARRIVLLNLGGLAALLVGFLWLN
                     QTRESVIDARVQSLTTQAEIIAAAVAASATAETDTITIDPDRLLELQAGETAAPKDEA
                     QAALEFSINPEKVAGVLRRLVGQTRTRARLFDRDGVLLLDTRSFYSAGQILKMDLPPA
                     DEEETTLFERSWSAIKRRLGRMDVPTYEETPSGKSYQEVQRALGGVQASVVRVTTSGQ
                     TIVSVAVPVKRFRSVQGVLMVMTQEGDIDRVIADERLGILLVFMIAAGVMLILSLLMA
                     GAIAEPLRRLAGAADRVRRRTEARPEIPDFSERSDEIGELSGALRDMTQALYSRIEAI
                     ESFAADVAHELKNPLTSLRSAVETLPLARTEEARGRLMAVIQHDVRRLDRLITDISSA
                     SRLDAELQRSAAERIDLVRLLETVIGMANDAGRPDGVVIRFLSPPQGEATQFRILGHD
                     SRLVQVFNNLIDNARSFSKAGGEVRVRLKRVDNQIVVQVDDDGPGVPDHARDRIFERF
                     YTDRPAQGFGQNSGLGLSISRQIVGAHRGTIHAENRKDAEGRVLGASFVVSLPAEAP"
     gene            complement(4768..5466)
                     /locus_tag="PLQ11_07920"
     CDS             complement(4768..5466)
                     /locus_tag="PLQ11_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003609400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRK24866.1"
                     /translation="MPTIALVDDDRNILTSVSIALEAEGYRIQTYTDGASALDGLKQN
                     PPDMAIFDIKMPRMDGMELLRRLRQKSDMPVIFLTSKDEEIDELFGLKMGADDFIRKP
                     FSQRLLVERVKAVLRRSGAKDAPPAKEGDVKTLERGQLRMDPERHACTWKGDAVTLTV
                     TEFLILQALAQRPGVVKSRNALMDAAYDDQVYVDDRTIDSHIKRLRKKFKIVDDDFEM
                     IETLYGVGYRFRES"
     gene            5603..6439
                     /locus_tag="PLQ11_07925"
     CDS             5603..6439
                     /locus_tag="PLQ11_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012252883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2470 domain-containing protein"
                     /protein_id="HRK24867.1"
                     /translation="MPILKAPAKTRRTNLEVPPMPLEPSLAAHMAPGFEPIVLSKQLL
                     RALRSGALASLTEAGMPFASLTSVATDYDGTPIILISRLATHTLNLERDPRCSLMLSQ
                     TGKGDPLAHPRLTLLAHAERAVPDSPLQMRIRTRFLNRHPKAALYADFPDFSFWRLVP
                     DQAFLNGGFARAWDGPANTILTEVADPVAAAELEAGAIAHMNADHGDALGLYATRLAG
                     QPAGAWKASGLDPEGLDLMAGDRTARIAFSQPVTEPGPLRRALAALAEQARAATQAGA
                     AP"
     gene            6693..8297
                     /locus_tag="PLQ11_07930"
     CDS             6693..8297
                     /locus_tag="PLQ11_07930"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012172583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HRK24868.1"
                     /translation="MITSGAFNATVGANSFGFAKPRSITCNLEAAALYEEAVRRGEAN
                     VLAGGALVAETGTHTGRSPKDKFTVKDRTTADTVWWDQNQAMSVEHFDVLLADFKAHA
                     EGKDLYLQDLVGGADPTHQVRTRVYTEYAWHSLFIRNLLIRIGRDQIPGFVPDMTIVD
                     FPTFKADPKRHGCKSDTVIAMDLTRKIVLIGGTAYAGEMKKSVFTYLNFVLPQKAVMP
                     MHCSANVGKDGDVALFFGLSGTGKTTLSQDPNRILIGDDEHGWAPEGVFNFEGGCYAK
                     TIRLSKEAEPEIYSTTQRFGTVLENVVFDPDTRVADFDDESKTENTRCAYPIEFISNS
                     SPTGRAGQPKNIIMLTCDAFGVMPPIARLNDSEAMYHFLSGYTAAVAGTERGVTEPTA
                     TFSTCFGHPFMPRHPSVYGNLLRDLIAKHGVTCWLVNTGWTGGKPGVGSRMPIKVTRA
                     LLTAALDGSLNKVQFRKDSYFGFDVPAAVPGIPGDILNPVATWADKAAFEETARKLVA
                     MFEKNFKTFEAHVDANVRAAAPKMAA"
     gene            8431..>8935
                     /locus_tag="PLQ11_07935"
     CDS             8431..>8935
                     /locus_tag="PLQ11_07935"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRK24869.1"
                     /translation="MTPRDTVLAIVVAALWGFAFVPIRIGVGEMPPLVLTALRFFFSA
                     VPFVFFIRPPKAPAWLVVAFGLTLGVVKFGLLFTGMKLGMPVGLSSLVMQMQVFFTIL
                     LAALIGREMPTRLQIIATLIALAGLGVIGLSDQRGAPLLPFLMLVLAAFVWSIANMLS
                     RLAKGADM"
BASE COUNT         1606 a         2865 c         2756 g         1708 t
ORIGIN      
        1 atggcggatg cgcaaccatc aaatcccatt cttcacccaa caaatcccga acggcagaaa
       61 agcgagggcc gccctttcgg ggcggccctt tcgttatggt tctcgccgaa agatcagtag
      121 cggtaatggt ccggcttgaa cggaccctgt tcagagacgc cgatataggc agcctggtcg
      181 ggccggagcc tggtcagctt gacgccaatc ttttcgagat ggagcgcggc aactttctcg
      241 tcgagatgct tgggcagagt gtagacggtc ttgtcatact tgcccggatt gcagaacaat
      301 tcgatctgcg ccagcacctg gttcgagaaa gaggcggaca tcacgaaaga gggatgcccc
      361 atggcattgc cgagattcac cagccgccct tcagacagca ggatgatgcg ccggccggtg
      421 gggaattcga tctcgtcgac ctgcggcttg acgttgtgcc acttgtaatt cttcagcccg
      481 gcgacctgga tctcgttgtc gaaatggccg atgttgcaga cgatcgcccg gtccttcatg
      541 ccgcgcatgt ggtcgatggt gatcacatcc ttgttgccgg tggcagtgac gaagatgtcg
      601 gcgcggccga ccgcatcctc catcgtggtc acctcgtagc cttccatcgc cgcctgcaaa
      661 gcgcagatcg ggtcaatctc ggagacgagc acgcggcagc cggcctggcg cagcgaggcg
      721 gccgaaccct tgccgacgtc gccgaagccg gcaaccatcg ccaccttgcc ggccatcatc
      781 acgtcggtgc cgcggcggat gccgtcgacc agcgattcac ggcagccgta gagattatcg
      841 aacttcgact tggtcaccga atcgttgacg ttgatcgccg ggaacagcag cttgccttcc
      901 ttggccatga tgtaaagccg gtggacgcct gtggtagtct cctcggagac acccttgatc
      961 gacttggcca gcgccgcgaa ccagccaggc ttttccttga gcgtgcgctt gatcagcgcg
     1021 aacaggattt cctcttcctc cgaacccggc ttgtcgagga acgccgtgtc gccctgttcg
     1081 gcgcgcaggc cgagatggac cagcagcgtg gcatcgccgc catcatcgag gatcatgttg
     1141 ggcgtgccgc catcacccca ttcgaggatc ttgcgggtgt aatcccagta ttcggtcagc
     1201 gtctcgccct tgaccgcgaa caccgggatg ccgccggccg cgatcgccgc agccgcatgg
     1261 tcctgggtcg aatagatatt gcacgaggcc cagcgaatgt cggcgccaag cgccttgagc
     1321 gtctcgatca gcacggctgt ctggatcgtc atgtgcagag agccggcgat tcgggccccc
     1381 ttgagcggct gccgcgtgcc gtattccttg cggatcgcca tcaggcccgg catttcggtt
     1441 tcggcgatgg caatctcctt gcgaccgaag tcggcaaggc caatatccct gacaatgtaa
     1501 tccatggctg tgtcccgatg ttgaaagctg gcggcgtata gcagggccag ccccgtcttt
     1561 caacaaagat ataaagacat gtttatattg tgatgaagtt tttggcgccg gcatggcaga
     1621 gcagtctctg ccgggccgac agagcggatc ggcggcgtgt cacaccacgg aaaaacgacg
     1681 aggtaagcgg tgcgacagga aatgaaacgt cactcgtctt caccgaaccg gtcggcaatg
     1741 agttgggcca gcgcatcgat tgcctgccgg gcatcctcac cgaccgccgc aacggtgatg
     1801 ctggtgccct gtgccgcggc caaagtcagc agccccatga tcgacgttcc acccactgtc
     1861 tcgccacagc gatgcacggt gactgccgag gcgaaccgat ccgcgcattg cacgaatttg
     1921 gccgatgcgc gggcgtgcag ccccttgcgg ttgatgatcg gcatcacctt gatgatggcc
     1981 ccttccggga agcggtcatc aggacagggt tcggtctcgg tcattttccc tgcagcacct
     2041 tgctggcgat gttgatgtat tttcggccgg cttcctgcgc tccggtgacg gcctgatcga
     2101 gatcgttgca ttcgcgcagc gacgccagct tgaccagcat cggcagattg atgccggcca
     2161 ccacttcgac acggtcaccg ttcatcgccg aaatcgcaag gttggagggt gtcccgccga
     2221 acatgtcggt aagcacgaca acaccgtgtc cggaatcgga cctggctatg gcctcaacga
     2281 tttccttgcg gcgcatttcc atatcatcgt ccgggccgat gcagatggtg gccacctgcc
     2341 tttgctgccc aaccacgtgc tccatggccg ccttgaattc gagcgccagg cggccgtggc
     2401 tcaccagaac caatccgatc atgtcgagac ccaggacccc cgggcactgc cgtcagcgac
     2461 cggttccgaa aacggcggtc attgtgtgcg ggcgcgagaa aagttcaagt gctttctcag
     2521 gccggccgaa acattgtgtt gcgatcaagc tgctgcagcc gttcctgaac cagcgcgaca
     2581 gcgaacgggc cgatgtcgcc gtcagacagc cgcaggagag gcagggcaat gccttcaaga
     2641 cggacgtgaa ggtccgatgg cggcatgcgc tcggcagggg ttgcgcccag ttcgatcacc
     2701 agtgacaggc gggctgtcgg ctcgtagttg ccagcgacca gccccaggcc gcgcaattcg
     2761 cgccggccgg cgatggcggg atgaaccgtg gccagcagcc gcccgtgccg ggcaaccagc
     2821 cgcaaccggt catcgccgac caggcaggcg aaccgtctcg ctgccgcctc ggcgcgcagc
     2881 agcaattcag ccaggcgtga cttgccggag ccggagacgc cacgaatcaa caccccctgt
     2941 tcgccgatca gcacgcaggt ggcatggctg tagcggtccg tgctcatgga gcctccgccg
     3001 gcaggctgac gacgaaactg gctcccagaa cccgcccttc ggcatccttc cggttctccg
     3061 cgtgaatggt tccacgatgg gccccgacga tctggcgcga gatcgacagg ccaagtcccg
     3121 aattctgtcc aaagccctgc gcgggccgat ccgtgtagaa gcgctcgaaa atgcggtcgc
     3181 gcgcgtggtc gggaacgcca ggcccgtcgt cgtcgacctg cacgacgatc tggttatcta
     3241 cccttttcag tctgaccctc acctcgccac cagccttcga aaacgaccgg gcattgtcga
     3301 tcagattgtt gaacacctgg accaggcgcg aatcatggcc aagaattcgg aactgtgtcg
     3361 cctccccctg aggcggtgac agaaaacgga tgaccacgcc gtccggccgt ccagcatcgt
     3421 tggccatgcc gatgaccgtt tccagcaacc gcaccaggtc gattcgttcg gcggcagagc
     3481 gctggagttc ggcatcaagc cgggaggccg atgaaatatc ggtaatcagc cggtcgaggc
     3541 gtcgcacatc gtgctggatc accgccatca accggccacg ggcctcctca gtgcgggcca
     3601 gtggcagggt ttccaccgca ctgcgcagtg aagtgagcgg attcttgagt tcatgcgcga
     3661 catcggcggc aaagctctcg atcgcctcga tgcggctgta gagcgcctgg gtcatgtcgc
     3721 gcaaggctcc cgacaattcg ccgatttcgt ctgaccgttc ggaaaaatcg ggaatctcgg
     3781 gccttgcctc ggtgcgccgg cggacgcggt cagcggcgcc cgccagccgt cgcagcggct
     3841 cggcgatggc accggccatc agcagcgaca ggatcagcat gacaccggcg gcaatcatga
     3901 agacaagcag aatccccagc cgctcgtcgg caatcacccg gtcaatgtcg ccctcctggg
     3961 tcattaccat cagcacgccc tgcaccgatc ggaagcgctt gaccggcacg gcgacggaca
     4021 cgatcgtctg gccggatgtt gtcacccgga cgaccgaggc ctgcacgccg ccaagtgcgc
     4081 gctgcacttc ctgataggat ttgccgcttg gcgtctcttc gtaggtcggc acgtccatcc
     4141 gccccaggcg gcgcttgatc gcactccagg agcgctcgaa cagcgtggtc tcctcttcgt
     4201 cggccggagg cagatccatc ttgaggatct ggccggccga atagaagctc ctggtatcca
     4261 gcagcagcac gccgtcccga tcgaacaggc gggcgcgggt gcgcgtctgc ccgaccaggc
     4321 gccgcagcac gccggcaacc ttctccggat tgatcgagaa ttccagcgcc gcctgggcct
     4381 cgtccttcgg cgccgcggtc tctccggcct gcagttcgag cagtctgtcg ggatcaatgg
     4441 taatcgtgtc tgtctctgca gtggccgagg cggcgacggc tgccgcgatg atctcggcct
     4501 gggtcgtcag agactgcacc cgggcatcaa tcacactttc gcgcgtctgg ttaagccaca
     4561 ggaagccgac cagcagcgct gccagacccc cgaggttgag caggacgatg cggcgggcaa
     4621 ccgtcgatgt aaggggcttc cagatcggct gccacaatcg ccggatccag cgtcgccgca
     4681 agccggacgc ccgctgcgcc gggcgatcct gcgcgcgggt cggcggggta tggcggtcct
     4741 gttggtccag caccgtgcct ccggccgtca gctttcgcgg aaccggtagc cgacaccgta
     4801 aagcgtttca atcatttcaa aatcgtcgtc gacgattttg aacttcttgc gcagccgttt
     4861 gatgtggctg tcgatggtcc gatcatcgac atagacctga tcgtcatagg ccgcgtccat
     4921 cagagcattg cggctcttga ccaccccggg ccgctgcgcc agcgcctgca ggatcaggaa
     4981 ctcggtcacg gtcagcgtca ctgcgtcgcc cttccaggtg caggcatggc gctccggatc
     5041 catgcgcagc tggccgcgct ccagggtctt gacgtcgccc tccttggccg gcggggcatc
     5101 cttggcgccc gagcgacgca ggaccgcctt gacccgttcg accagcagcc gctgcgagaa
     5161 cggtttgcgg atgaagtcgt cggcgcccat tttgaggccg aacaattcat cgatctcctc
     5221 gtcctttgag gtgaggaaga tcaccggcat gtcggatttc tgtctcaggc gacgtaacag
     5281 ttccatgccg tccatgcgcg gcatcttgat atcgaagatc gccatgtcgg gcggattctg
     5341 cttgagaccg tcaagcgcgc ttgcgccatc cgtataggtc tggatgcggt agccctccgc
     5401 ttcaagcgcg atcgagacgg aagtaaggat gttgcggtcg tcatcgacca gggcaatcgt
     5461 tggcatggtg gttcctgaaa gtgaacggct ggctgccgcc gcgattatag cggagtttgc
     5521 ttgtgcgaac aaacgcaggc cgaattgtgg cgtccgcaac ggcgaggccg gaccatggca
     5581 atggcttgcg caaggcgcgg tcatgcctat cttgaaggca cccgccaaaa cccggcgaac
     5641 caacctggag gtgccgccga tgccgctcga accgtccctt gcggcccata tggcgccggg
     5701 ctttgaaccg attgtcctgt ccaagcagct gctgcgcgcg ctgcgatcgg gcgcgctggc
     5761 aagcctgacc gaggccggta tgccctttgc gtcgctgacc tccgtggcga ccgactatga
     5821 cggaacgccc atcatcctga tttcgcggct ggctacccac accctgaacc tcgagcgtga
     5881 tccgcgctgt tccctcatgc tctcgcaaac cggcaagggg gatccgctcg cccacccgcg
     5941 cctgaccttg ctggcgcatg ccgaacgggc ggtgccggac agtccgctgc agatgcggat
     6001 caggacacga tttctgaacc gccaccccaa ggcggcgctt tatgccgatt tcccggattt
     6061 ctcattctgg cggctggttc cggatcaggc ctttctcaac ggcggcttcg cccgcgcctg
     6121 ggacgggccg gcgaacacaa tcctgaccga agtggctgat cctgtcgcgg ccgcagagct
     6181 cgaagcgggg gcgatcgcgc atatgaatgc cgatcatggc gatgcgctcg gcctctatgc
     6241 cacccggctg gccggtcagc cggcgggcgc ctggaaggcc agcggcctcg atccggaggg
     6301 gctggacctg atggccggcg accggacggc gcggattgcc ttttcccagc cggtcaccga
     6361 acccggcccg ctgcggcgcg ccttggccgc cttggccgag caggcacggg ccgcgacgca
     6421 ggcaggtgcg gcgccctgat gtctgacaac tgacctcaaa ttctgtagaa ttttatttca
     6481 ggaagccact tatcctaagg atcaatgtga caaatctgct gaaattcggc gcttgcgatt
     6541 ggcaaaagcg caaattctgc taggggacgc gacgtcgggt aacgtggtgt tcacgccaca
     6601 gatccaaggt cttgcctggc cgtgaatgca tccggccggc aggacgctga gcgccgcatc
     6661 aggcgcacaa tgtgcaggaa ggacggagtt tcatgatcac cagcggcgct ttcaatgcca
     6721 ccgtcggagc caacagcttc ggttttgcca agccccgttc gatcacctgc aatcttgagg
     6781 ccgcggccct ctatgaggag gcggttcgac gtggcgaggc caatgttctg gccggcggcg
     6841 cgctcgtggc ggaaaccggc acccacaccg gccgcagccc caaggacaag ttcaccgtca
     6901 aggaccgcac caccgccgat acggtgtggt gggatcagaa tcaggcgatg tcggtcgagc
     6961 atttcgatgt gctgctggct gacttcaagg cccatgccga aggcaaggat ctctatctgc
     7021 aggatctggt cggcggcgcc gatccgaccc atcaggtgcg cacccgggtc tataccgaat
     7081 acgcctggca ctcgctgttc atccgcaacc tgctgatccg cattggccgc gaccagattc
     7141 cgggctttgt gccggacatg acgatcgtcg atttccccac gttcaaggct gatcccaaac
     7201 gccacggctg caagtcggac accgtgatcg ccatggatct gacccgcaag atcgtgctga
     7261 tcggcggcac cgcctatgcc ggcgagatga agaagagcgt cttcacctac ctcaacttcg
     7321 tgctgccgca gaaagcggtg atgccgatgc attgctcggc caatgtcggc aaggacggcg
     7381 acgtcgccct gttcttcggc ctttcgggca ccggcaagac caccctgtcg caggacccca
     7441 accgcatcct catcggcgac gacgagcatg gttgggcacc cgagggcgtg ttcaatttcg
     7501 aaggcggctg ctacgccaag accatccgcc tgtcgaagga ggcggagccg gaaatctact
     7561 cgaccaccca gcgcttcggc acggtgctgg aaaatgttgt gttcgatcct gatacccgcg
     7621 tggccgattt cgacgacgaa tcgaaaaccg agaacacccg ctgcgcctat ccgatcgaat
     7681 tcatctccaa ttcctcgcca acggggcgtg ccggtcagcc gaaaaacatc atcatgctga
     7741 cctgcgacgc cttcggtgtg atgccgccga tcgcccggct gaacgattcc gaagcgatgt
     7801 atcacttcct gtcgggctac accgcagcgg tcgccggcac cgagcgcggc gtcaccgaac
     7861 cgaccgcgac cttctcgacc tgtttcggcc atccgttcat gccgcgccac ccgtcggttt
     7921 acggcaacct gctgcgcgat ctgatcgcca agcatggggt gacctgctgg ctggtcaaca
     7981 ccggctggac cggcggcaag ccgggcgtcg gctcgcgcat gccgatcaag gtgacccgtg
     8041 cacttctgac cgccgccctc gatggctcgc tcaacaaggt ccagttccgc aaggattcct
     8101 atttcggctt tgatgttccg gcggccgtgc cgggcattcc cggcgatatc ctcaacccgg
     8161 tcgcgacctg ggcggacaag gctgccttcg aggagacggc acggaagctg gtcgcgatgt
     8221 tcgagaagaa cttcaagacc ttcgaagccc atgttgacgc caacgtgcgt gccgccgccc
     8281 cgaagatggc cgcctgacaa ggcgactgcc ggacgggcac ctcgccgatc cggccaatgc
     8341 ccgttgccgg ttacgggtga cgggctcctg ctttggtatt cagccgggat cggctcatga
     8401 tggtccggtg attcgctcac cggacattcc atgaccccgc gcgatacggt tctggccatc
     8461 gtggtcgcgg cgctgtgggg ctttgccttc gtgccgatcc gcatcggcgt tggcgagatg
     8521 ccgccactgg tgctgaccgc cttgcggttc ttcttctctg cagttccgtt cgtgtttttt
     8581 atcaggccgc ccaaggcgcc ggcctggctg gtggtcgcct tcgggctgac cctgggcgtg
     8641 gtcaagttcg gcctgttgtt caccggcatg aaactcggca tgccggtggg gctgtcgtcg
     8701 ctggtcatgc agatgcaggt gtttttcacc atcctgctgg cggcgctgat tggccgggaa
     8761 atgccgacgc ggttgcagat catcgcgacg ctgattgcgc ttgctggcct tggagtgatc
     8821 ggcttgtcgg atcagcgcgg ggcgccactg ctgccgttcc tgatgctggt ccttgccgca
     8881 tttgtctgga gcatcgccaa catgctgtcg cggctggcaa agggggcgga catgc
//
LOCUS       DAOMXG010000101         8802 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_4207_length_8802_cov_12.473762, whole genome shotgun sequence.
ACCESSION   DAOMXG010000101 DAOMXG010000000
VERSION     DAOMXG010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 8802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8802
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4207_length_8802_cov_12.473762"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="PLQ11_07940"
     CDS             <1..233
                     /locus_tag="PLQ11_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24870.1"
                     /translation="GRSYHLTIAWTRGILAQGKGVFTQQTVWFARGKVKAVGQNHRAS
                     CGEMKMGRNPTAFACRYRSISASQSRLLASPF"
     gene            complement(372..1061)
                     /locus_tag="PLQ11_07945"
     CDS             complement(372..1061)
                     /locus_tag="PLQ11_07945"
                     /inference="COORDINATES: protein motif:HMM:NF005679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HRK24871.1"
                     /translation="MSAPRIGILSLDTRFPRIAGDVGNPDSYPFPVRIERVAGATPER
                     VVHQRAEGLVEAFAAVGQRLAAEGCEALVTTCGFLSLRQSELAAALPVPVATSALMQL
                     PLIQQMLPAGRITGIVTASERSLTPAHLTGVGADPQTPIVGVEEGGAFSATFVGNGDR
                     LDVAAARTEVVAAALRLVATQPRLGAILLECANMGPYARDVQHATHLPVFDMVGLVRW
                     LHQALDQPAYL"
     gene            complement(1101..2078)
                     /locus_tag="PLQ11_07950"
     CDS             complement(1101..2078)
                     /locus_tag="PLQ11_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRK24872.1"
                     /translation="MLRRTVLAGLSLAALVAVSGPAAAQTKWNLPSAYPANNYLTENL
                     VQFGKDIEAATGGKIGVTVHANASLFKAPEIKRAVATGQAQLGEVLMSLHENEDPIFG
                     IDVVPFLATSFAQSRKLWEVSRPSVEKKLEAQGLKVLFAQAWPPQGIYAKKDLNSVED
                     LKGVKFRSYNPGTARIAELSGAQPVTIQAAELPQALATGVVQSFISSGATGYDSKVWE
                     SLTHWYDTQAWIPKNLIFINKAVFDALPKDQQDAVMKAARDAEARGWTTVAEKAGWYI
                     NELKAKGMKVQPPGDKLAADLKKLGETLTADWLKKAGAEGQAVVDAFRK"
     gene            complement(2176..3480)
                     /locus_tag="PLQ11_07955"
     CDS             complement(2176..3480)
                     /locus_tag="PLQ11_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRK24873.1"
                     /translation="MTLVQLSVILLMFMVAILASGVWISIGLALVGFLAMVLSTNVPI
                     GNVLATSVWSASASWTLAALPLFIWMGEILFRTKLSEEMFRGLAPWLQWLPGRLMHVN
                     VVGCGIFAAVSGSSAATCATIGKISLPELEKRGYDPSMSLGSLAGSGTLGLLIPPSIP
                     MVVYAITANVSVLQVFLAGFIPGAMVMALYSGYIMVWSLLNPDKTPPRDPPLPFLGKV
                     RESTKLIPTLLLILGVFLSLILGIATATECAAWGVFGALVLAWWSGTLSWGTFTDSLL
                     SATRLTGMIMLILAGAAFTTAAMAYTGIPAALAAWVEAKQLSPAMLIVALTVMYIILG
                     CLIDGISMIVLTAVIVLPMIQKAGFDLVWFGVYLIILVEMAQITPPVGFNLFVLQNMA
                     NKDTFTVAKAAFPFFLLLNVAVLIIYLFPEIVLYLPKKVFPD"
     gene            complement(3484..4050)
                     /locus_tag="PLQ11_07960"
     CDS             complement(3484..4050)
                     /locus_tag="PLQ11_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008132152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRK24874.1"
                     /translation="MSHRTGAVRRILDRLYLWGGYAAGAFLLITFVLMIVMSAGREVG
                     LNVPSGDDFAAFSMAALAFLGLANVFKTGEMIRVGFLIERIHGPARRAIEIFCLSVGF
                     AFIAYFAWNAFFFMRDSWRLHDMTSGAIAIPLWIPQISMVLGLTLLAIAMLDELIIVL
                     SGRKPSYEKEPPKTKEELLERIIESGGV"
     gene            complement(4108..5121)
                     /gene="corA"
                     /locus_tag="PLQ11_07965"
     CDS             complement(4108..5121)
                     /gene="corA"
                     /locus_tag="PLQ11_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HRK24875.1"
                     /translation="MLADGARSLSMPDDGPGVVAAGAYAKGHRVANIAIEEAGEWAHR
                     PGHVVWIGLLEPDDDLLARVQRQFGLHDLAIEDARNAHQRPKLEQYGNGLFVVARTAQ
                     MIDGRVAFGETHLFVGKGYVVSVRHGASKTYSSVRGRCEANPNQLSHGEDFILYAILD
                     FIVDNYMDVLEAMHEEVELIEDIVLSKALSAAEVERLYTLRRDLLRLRNAVVPLVDVC
                     RRIENTEHLAVDDVMRHHFRDVTDHIRRVQEEIESLREVLAFAFEASLMGGQAQQTEV
                     SRKLSAGAAILAVPTAIAGIYGMNFKNMPELESQYGYYIVLAVIISFCTLLFWRFRKN
                     GWL"
     gene            complement(5179..6075)
                     /gene="argB"
                     /locus_tag="PLQ11_07970"
     CDS             complement(5179..6075)
                     /gene="argB"
                     /locus_tag="PLQ11_07970"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HRK24876.1"
                     /translation="MTKLSLPDVHVQAEVISQALPHMQRYDQQTVVIKYGGHAMGDKH
                     AQEDFAEDVVLLEQAGVKPIVVHGGGPQIGRMLDKLGIKSEFRAGLRVTDQATVEVVE
                     MVLAGSINKQMVGWIAAEGGKAIGLCGKDGSMVTARKLTRTMVDPDSNIEKVVDLGFV
                     GDPHTVNRAILDAVLKAELIPVIAPVAVGDDGQTYNVNADTFAGAIAGAMRAKRLLLL
                     TDVPGVLDKNKQLIETLTVEDCRRLIADGTVTGGMIPKIETCIYAIERGVEGVVILDG
                     KVPHAVLLELLTDHGAGTLITR"
     gene            complement(6201..6575)
                     /locus_tag="PLQ11_07975"
     CDS             complement(6201..6575)
                     /locus_tag="PLQ11_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011923959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HRK24877.1"
                     /translation="MTATRIHCALACLFGAAGVVLMALGAHVAGSLSATAGQMLLIHA
                     PAVLAATVARKAGFLADRLALLGIAALLLGPALFAGDLAMRELAGNRLFPMAAPVGGA
                     LAIAGWVLLTIAALTAPRNPVR"
     gene            complement(6572..7216)
                     /gene="yihA"
                     /locus_tag="PLQ11_07980"
     CDS             complement(6572..7216)
                     /gene="yihA"
                     /locus_tag="PLQ11_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HRK24878.1"
                     /translation="MAVGEDFTEAGRRLFAGEAGFFWAASSLASLPPPSGIEIAFAGR
                     SNVGKSSLINALTGRKALARTSNTPGRTQDLNFFRVDDQLTLVDMPGYGFAAVGKQKV
                     AAWTGLIHDYLRGRTNLARVLLLIDARHGLKATDRPVLETLATSAVSYQVVLTKADAL
                     GAGALRARVAEMQAVLRKEAAAFPEIIVTSSKDGTGMAQLRAAIARLISERTGR"
     gene            complement(7231..>8802)
                     /gene="yidC"
                     /locus_tag="PLQ11_07985"
     CDS             complement(7231..>8802)
                     /gene="yidC"
                     /locus_tag="PLQ11_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HRK24879.1"
                     /translation="LKSRAEALAGSQRIAIDTPALKGSVSLRGGRFDDLSFVRYRETI
                     DPKSANIVLFSPSDADKGYYADIGWVAPSGSAVAAPNAETLWQSPGGVLTPSSPVTLT
                     YDNGQGLFFTRTLQVDDKFMFTVTDRVENRGSGPVALSPYGRISRIGAPPVEGIYVLH
                     EGLIGYMGDQKLQEIVYKDMDKMKSKTFKDVTGGWLGITDKYWASAIIPDQDKTYSAA
                     FGVTAGQPATYETAAVLASMTIAPGASVQSVTRLFAGAKEVEVIDRYESQFKITSFDK
                     TIDWGWFHFLTRPLFKLMDMINKVFGNFGVAILIVTVIVKLVFFPLANKSYRSMAMMK
                     DAAPEMQAIRERFADDRVRQQQEMMELYKKKQINPMAGCLPILVQIPVFFALYKVIYI
                     TIEMRQAPFFGWIKDLSAPDPTSIFNLFGLLPYAVPNWSMFQVGVWPVLMGITMWLQM
                     KMNPEPPDPVQKQIFAWMPLIFTFMLGSFPAGLVIYWAWNNLLSVIQQYVIMRQQGVK
                     VELWDNLKGSLGRKK"
BASE COUNT         1810 a         2764 c         2687 g         1541 t
ORIGIN      
        1 taggtcgtag ttaccattta acgattgcct ggacgcgagg gattttggct caaggcaaag
       61 gcgttttcac gcagcaaacc gtctggtttg caagaggaaa ggtcaaagcc gtgggccaaa
      121 atcaccgcgc ctcgtgtggt gagatgaaaa tgggccgcaa tccaaccgcc ttcgcttgcc
      181 gatatcgctc gatatcggca tcgcaaagcc ggctgcttgc gtccccattt tgatctcatc
      241 cagacagtcg ttaaatggta actacgacct agagcatttt cagtgaattc caattcacag
      301 aaagtgctct atctttttgt tttttcgcat tttcttaacg cgaaccggaa tccacttcgc
      361 ttgaaaatgc tctaaagata ggcgggctgg tcgagcgcct ggtgcagcca gcgcaccagc
      421 ccaaccatat cgaaaaccgg caaatgcgtg gcatgctgaa catcgcgggc gtaaggcccc
      481 atgttggcgc attcgagcag gatggcacca agccgcggtt gggtcgcaac cagtcgcagt
      541 gcggcggcaa ccacttccgt gcgggccgca gcgacatcaa gacgatcgcc attgccgaca
      601 aaggtggcag agaacgcccc gccctcctca accccgacaa tcggcgtttg cggatcggcg
      661 ccgacgccgg tcaggtgggc cggcgtcagc gatcgctcgg atgcggtgac aatcccggtg
      721 atccgcccag ccggcaacat ctgctgaatc agcggcagct gcatcagcgc cgaggtcgcc
      781 accggcactg gcagggccgc cgccagttcc gactggcgca gggacaggaa accgcaggtg
      841 gtcaccagcg cttcacatcc ctcggcagcc agccgctgtc cgactgccgc gaacgcttca
      901 acgaggccct cagcacgctg atgaacaacc cgttcaggcg tggccccggc gacccgttcg
      961 atgcggacgg gaaacggata gctgtccggg ttgccgacat cgccggcgat gcgcggaaag
     1021 cgtgtatcca gtgacagaat gccgatgcgg ggcgctgaca tgtcaggcgc cccgcatcct
     1081 gccgttcatg tccgttttgc tcacttcctg aaagcgtcga ccacggcctg gccttccgcg
     1141 ccggccttct tgagccagtc ggcggtgagc gtttcgccca gcttcttgag atctgcggcc
     1201 agcttgtcgc cgggcggctg aaccttcatg cccttggcct tgagctcatt gatataccag
     1261 ccggcctttt cagccaccgt cgtccagccg cgcgcctcgg catccctggc cgccttcatc
     1321 accgcgtcct gctgatcctt cggcagggca tcgaacaccg ccttgttgat gaagatcagg
     1381 ttcttgggga tccatgcctg ggtatcatac cagtgggtca ggctttccca gaccttggaa
     1441 tcgtaccccg tcgcgcccga tgaaatgaac gattgcacga cgccggtagc cagcgcctgc
     1501 ggcagttccg cagcctggat ggtcaccggc tgggcgccgg agagttcggc gatgcgcgcg
     1561 gtgccggggt tgtaggagcg gaacttgacg cccttgagat cctcgaccga attgagatcc
     1621 ttcttggcgt agatgccctg cggcggccag gcctgcgcga acagaacctt caggccctgc
     1681 gcctccagct tcttctcgac ggagggacgc gaaacctccc acagcttcct ggactgggca
     1741 aaagaggtcg caaggaacgg caccacatcg atgccgaaga tcgggtcctc gttttcgtgc
     1801 aggctcatca gcacctcgcc gagttgcgcc tgtcccgtcg cgaccgcgcg cttgatctcc
     1861 ggcgccttga acagcgaggc attggcgtga acggtgacgc cgatcttgcc accggttgcc
     1921 gcctcgatgt ccttgccgaa ctgaacgaga ttttcggtca gatagttgtt ggcaggatag
     1981 gcgctcggca ggttccactt ggtctgggcg gcggcgggtc cgctgacggc gacaagggcc
     2041 gctaaggaaa gtccggccag aacggtgcga cgaagcatgg ttttctccct gatatcttga
     2101 ggcaatgctg ccttggcaac agtcaaccgc gaaacccagc gcggtgcaag cgcgctgacg
     2161 catgttcctg ccagctcaat cgggaaaaac cttcttgggc aggtagagga cgatctccgg
     2221 aaagaggtag ataatcagca cggcaacgtt gagcagcagg aaaaacggaa acgctgcctt
     2281 ggcgactgtg aaggtatcct tgttcgccat gttctgtagc acgaacagat tgaacccgac
     2341 gggcggcgtg atctgggcca tctcgaccag gatgatcaga taaacgccga accagaccag
     2401 atcaaacccg gccttctgga tcatcggcag cacgatcact gccgtcagca cgatcatcga
     2461 aatgccgtcg atcaggcagc cgaggatgat atacatgacg gtcagggcga caatcagcat
     2521 ggcgggcgag agctgcttgg cctcgaccca ggcggcaagc gcagccggaa tgccggtata
     2581 ggccatggct gcggtggtga aggcggcgcc ggccagtatc agcatgatca tgccggtgag
     2641 gcgcgtcgcc gacagcaggc tgtcggtaaa tgtgccccag ctcagcgtgc cggaccacca
     2701 ggcaaggacc agtgcgccga aaacacccca ggcggcgcat tcggtcgcgg tggcaatgcc
     2761 gagaatcaac gacaggaaca cgccgagaat gagcaacagg gtcgggatta gcttggtcga
     2821 ttcccgcacc ttgccgagaa acggcaatgg tggatcacgc ggcggtgtct tgtccgggtt
     2881 caacagcgac cagaccatga tgtagccgga gtagagcgcc atcaccatcg cgcccggaat
     2941 gaagccggcg agaaacacct gcaacaccga gacgttggcg gtgattgcgt agacgaccat
     3001 cgggatcgac ggtggaatca gcaggccaag cgtcccggag ccggcgagcg agccgaggct
     3061 catcgacggg tcataacccc gcttttcaag ttcaggcagc gaaatcttgc caatggtggc
     3121 gcaggtcgcc gcagaggaac cggagacggc agcgaaaatg ccgcagccaa cgacattgac
     3181 atgcatcagc cgccccggca gccactgcaa ccacggcgcc aggccgcgga acatttcctc
     3241 ggacaatttg gtgcggaaca ggatttcgcc catccagatg aacaacggca gcgccgcaag
     3301 cgtccaggag gccgaggctg accataccga tgtcgccagc acgttgccga tcggcacatt
     3361 ggtcgacagg accatggcca gaaatccgac cagggcaagg ccaatcgaaa tccatacgcc
     3421 gctggcgaga atcgccacca tgaacatcag caggatgacg gagagctgga cgagcgtcat
     3481 ggatcacacg cctccgctct cgatgatgcg ttcgagcagc tcttccttgg tcttgggcgg
     3541 ctccttttca tagctcggct tgcgtccgga caggacgatg atcaattcgt ccagcatcgc
     3601 gatggccagc agcgtcaggc cgagaaccat cgaaatctgc ggaatccaca gtgggatcgc
     3661 gatggcgccg gaggtcatgt catgcaggcg ccaggagtca cgcatgaaga aaaaagcgtt
     3721 ccaggcgaaa taggcgatga aggcgaagcc gaccgaaagg cagaagatct ctatcgcgcg
     3781 ccgtgccggt ccatggatcc gctcgatcag gaagccaacc cggatcatct cgccggtttt
     3841 gaagacattg gcgaggccga gaaacgccag cgccgccatc gagaaggcgg caaaatcatc
     3901 gcccgagggg acattcaggc caacctcccg gccggccgac atgacgatca tcaagacaaa
     3961 cgtgatcagc agaaatgcgc ccgcagcgta gccgccccac agatagagac gatccagtat
     4021 gcggcgtacg gcccccgtcc gatgtgacat cgctctcccc ctcgggcgag agattgaccc
     4081 gcgatgcccg gtctgccaag cgcaaagtta cagccagccg ttctttcgga agcgccagaa
     4141 cagcaacgtg cagaacgaga tgatcaccgc cagcacgata taatagccat actggctttc
     4201 aagttccggc atgttcttga aattcatgcc gtagatgccg gcgatagccg ttggaacagc
     4261 aagaatggcc gcaccggcgg agagcttgcg ggaaacctcg gtctgctgcg cctggccacc
     4321 cataaggctg gcttcaaagg cgaaggcaag cacctcgcgc aatgattcga tctcttcctg
     4381 aacgcggcgg atgtgatcgg tcacgtcgcg gaaatggtgg cgcatcacat catcgaccgc
     4441 cagatgctcg gtgttctcga tgcggcgaca gacatcgacc agcggcacca ccgcattgcg
     4501 caggcgcaga agatcacggc ggagcgtata gagccgctcg acctcggccg ccgacagcgc
     4561 cttggacagc acaatgtcct cgatcagttc gacctcctca tgcatcgcct caagcacgtc
     4621 catgtaattg tcgacgatga aatcaaggat ggcatagagg atgaaatcct cgccgtggct
     4681 gagctggttg gggttggcct cgcagcggcc gcgcaccgaa gaataggtct tggacgctcc
     4741 gtgccgcacc gagaccacat agcccttgcc gacgaacaga tgtgtttcgc caaaggcaac
     4801 gcgcccgtcg atcatctggg cagtgcgggc gacgacgaac aggccattgc cgtattgctc
     4861 gagcttgggc cgctggtggg cattgcgggc gtcctcgatc gccagatcat gcaggccgaa
     4921 ctgtctttga acgcgcgcca gcagatcgtc atccggctca agcagaccga tccagacgac
     4981 atggccgggc cggtgtgccc attcgccggc ctcttcgatc gcgatattgg caacccggtg
     5041 gcccttggca taggccccgg ccgcgacaac gcccggacca tcatccggca tcgacagcga
     5101 tcttgccccg tccgccagca tgcatccctc ccggctgttc tcgttggtcg tcaggcgggg
     5161 cggccggggg ccttcccgtc agcgggtgat cagcgtcccg gcgccgtgat cggtcagcag
     5221 ttccagaagc acggcatgcg gcaccttgcc gtcaagaatc acgacgcctt cgacgccgcg
     5281 ctcgatggca tagatgcagg tctcgatctt ggggatcatg ccgccggtca cagttccatc
     5341 ggcaatcagc cggcggcaat cctccaccgt cagagtttcg atcagctgct tgttcttgtc
     5401 gagcacgccc ggcacgtcgg tcagcagcag caggcgcttg gcccgcatcg ccccggcgat
     5461 ggcgccggcg aaggtatcag cattgacatt ataggtctgc ccgtcatcgc ccaccgcaac
     5521 cggcgcgatc accgggatca attcggcctt cagtacggca tcgaggatgg cccggttgac
     5581 ggtatgggga tcaccaacga acccaagatc aacgactttc tcgatgttgc tgtcgggatc
     5641 gaccatggtg cgggtcagct tgcgggcggt caccatcgag ccgtccttgc cgcacaggcc
     5701 aatcgccttg ccaccctcgg ccgcgatcca gccgaccatc tgcttgttga tcgagccggc
     5761 cagcaccatc tcgacaacct cgacggttgc ctggtcggtc acgcgcaggc cggcgcggaa
     5821 ttcgctcttg atgccaagct tgtcgagcat cctgccgatc tgcggcccgc cgccgtgcac
     5881 gacgatcggc ttgacgccgg cctgttcgag caacaccaca tcctcggcga aatcctcctg
     5941 ggcgtgcttg tcgcccatgg catggccgcc atatttgatg accactgtct gctggtcata
     6001 gcgctgcatg tgcggcaggg cctgactgat cacctcggcc tgaacgtgga catctggaag
     6061 cgacagcttg gtcatgcgcg ggcaccctca atgcggacag acaaacccag cagagcggct
     6121 tcttgcaaac aagacccgcg cacaggatca ggaaccatcc ataggccgga gcgtgcccgc
     6181 tgcaaagcct tctgttgcgc tcagcggaca ggattgcgcg gcgccgtcag ggcggcgatg
     6241 gtcagcagca cccagccggc aatggccagt gcaccgccga cgggggcagc catcggaaac
     6301 agccgattgc cggccagttc ccgcatcgcc agatcgccgg caaacagcgc cgggccaagc
     6361 agcaatgcag caatgccgag cagggccagg cgatcggcca ggaagcctgc ctttctcgcc
     6421 acggtggcgg cgaggacggc aggggcatga atcagcagca tctggccggc agtcgccgaa
     6481 agcgagcccg cgacatgggc accaagcgcc atcagcacca cgccagcggc cccgaacagg
     6541 caggccagtg cgcaatggat gcgggtagcc gtcatcggcc ggtcctttcg ctgatgaggc
     6601 gggcaatcgc cgcgcgcaat tgggccattc cggtgccatc cttcgacgag gtgacgatga
     6661 tctcaggaaa tgccgccgcc tccttgcgca gcaccgcctg catctcggcc acccgcgccc
     6721 gcagcgcgcc ggcgccaagc gcatccgcct tggtcagcac gacctgatag gacaccgccg
     6781 aggtggcaag cgtctcgagc accgggcgat cggtcgcctt caatccatgg cgcgcatcga
     6841 tcagcagcag aacacgggca aggttggtgc ggccgcgcag atagtcgtgg atgagcccgg
     6901 tccaggccgc gaccttctgc ttgccgaccg ccgcaaagcc atagcccggc atgtcgacca
     6961 gcgtcagctg gtcatcgacg cggaagaagt tgaggtcctg cgttctgccc ggcgtgttgg
     7021 aggtgcgggc cagcgccttg cggccggtca gggcattgat cagcgacgat ttgccgacgt
     7081 tcgagcgccc ggcaaaggcg atctcgatgc cggacggtgg cggcaggctt gcaaggctgg
     7141 aagctgccca gaagaaaccc gcctcgccgg caaacagccg gcgaccagcc tccgtgaaat
     7201 cctctcccac ggccatggcg ccctccggcg tcacttcttg cggccgagac tgcccttgag
     7261 attgtcccaa agctcgacct tcacgccctg ctggcgcatg atgacatatt gctgaatgac
     7321 cgaaagcagg ttgttccatg cccagtagat gaccagacct gccgggaacg agccgagcat
     7381 gaaggtgaag atgagcggca tccaggcgaa aatctgcttc tgaaccggat ccggcggctc
     7441 cgggttcatc ttcatctgca gccacatggt aatgcccatg agcaccggcc agacgccgac
     7501 ctggaacatc gaccagttcg gcacggcata gggcaggagg ccgaacaggt tgaaaatcga
     7561 ggtcggatcg ggcgccgaca ggtccttgat ccagccgaag aacggcgcct ggcgcatttc
     7621 gatggtgatg tagatgacct tgtagagcgc gaagaacacc gggatctgca cgaggatcgg
     7681 caggcagccg gccatcgggt tgatctgctt cttcttgtaa agctccatca tctcctgctg
     7741 ctggcgcacc cggtcgtcag cgaagcgctc gcggatcgcc tgcatctcgg gcgcagcgtc
     7801 cttcatcatc gccatcgagc ggtaggactt gttggccagc gggaaaaaga ccagcttgac
     7861 gatcacggtc acgatcagga tggcgacgcc gaagttgccg aacaccttgt tgatcatgtc
     7921 catcagcttg aacagcggcc tggtcaggaa gtggaaccag ccccagtcga tggtcttgtc
     7981 aaaactggtg atcttgaact ggctctcgta gcggtcgatg acctcgacct ccttggctcc
     8041 ggcaaacagc cgcgtgacgg attgaacgga ggcgccgggg gcgatcgtca tggaggccag
     8101 caccgccgcc gtctcatagg tcgccggttg gccggcggtg accccgaaag ccgccgaata
     8161 ggtcttgtcc tggtcaggaa tgatggccga ggcccagtat ttgtcggtga tgccgagcca
     8221 gccgccggtc acgtccttga aggtcttgga cttcatcttg tccatgtcct tgtagacgat
     8281 ttcctgcaac ttctgatcgc ccatatagcc gatcagcccc tcgtgcagca cgtagatgcc
     8341 ttcgaccggc ggagcgccga tccgcgaaat ccggccatag ggcgacagcg cgaccggtcc
     8401 cgatcccctg ttctccaccc tgtcggtgac cgtgaacatg aacttgtcat ccacctgcag
     8461 cgtgcgggtg aagaaaaggc cctggccatt gtcataggtc agcgtcaccg ggctcgacgg
     8521 agtgagcaca ccccctggcg attgccagag cgtctcggca ttcggcgccg caacagcgct
     8581 gccggaggga gcaacccagc cgatatcggc gtaatagccc ttatcggcat cagagggcga
     8641 gaacagcacg atattggcgc tcttgggatc gatggtctcg cgatacctga cgaaggacag
     8701 atcatcgaaa cgcccgcccc ggagcgacac gctgcccttg agcgccgggg tatcgatggc
     8761 gatccgctgc gagcccgcca gcgcctcggc acgcgatttc ag
//
LOCUS       DAOMXG010000102         8704 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_4259_length_8704_cov_8.789109, whole genome shotgun sequence.
ACCESSION   DAOMXG010000102 DAOMXG010000000
VERSION     DAOMXG010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 8704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8704
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4259_length_8704_cov_8.789109"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1099)
                     /locus_tag="PLQ11_07990"
     CDS             complement(<1..1099)
                     /locus_tag="PLQ11_07990"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24880.1"
                     /translation="MRLPSGGIVIGLVLGLALALAGCGLGLDSEQIVLCRQVLPVVAP
                     ERPATIVAEEAGEAGTVFVRFRTPGLAAEQLLACRFAGSGLGLDRRVLTGVAVNGSSL
                     SASAVFFLRERWLTSQDAVADAPALPRGTVTQLSPGVAYGLQQGLAALPKLGIYALIS
                     ASYAVIYGLVGRINLAFGGFAALGGSVAVLAMMLIDLLGLPSLSVGMVGGLWIAWMTA
                     TLYGLTAARLVIGRLTFRPGQGVLIASVGLSLVLSEGLRLAQGASALWLPPLLAAPIV
                     VAEAPGFVVTLTPMALLTAIMAGGSALAMAVALRRGRFGRDWHAVADDAVAAELFGID
                     PQEVLVQSFAIAAAMAGLAGALVSLHYGGMGF"
     gene            complement(1103..2119)
                     /gene="hemB"
                     /locus_tag="PLQ11_07995"
     CDS             complement(1103..2119)
                     /gene="hemB"
                     /locus_tag="PLQ11_07995"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019962549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HRK24881.1"
                     /translation="MNSPQRFTPASLDIARRPRRNRKSDWKRRLVREHALSVNDLIWP
                     IFIVAGEDRREPVASMPGVFRLSIDLAVKEVEEAARLRIPAVALFPYTDPKLRDERGS
                     EALNPQNLVCSACRQIRAKGLDIGIITDVALDPYTSHGHDGLMRDGDILNDETVAVLC
                     QQAVTQAAAGADIIAPSDMMDGRIGAIRAALDNAGFHKVSIMAYSAKYASAFYGPFRD
                     AVGANKTLTGDKKTYQMDPANGAEALVEAELDISEGADMVMVKPGMPYLDVIFRLKQG
                     FGMPTFAYQVSGEYAMIMAAAERGWLDREAAMLESLIAFKRAGADGILTYFAPAVARL
                     LAKD"
     gene            2277..3329
                     /locus_tag="PLQ11_08000"
     CDS             2277..3329
                     /locus_tag="PLQ11_08000"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRK24882.1"
                     /translation="MTSEAEILIRREGAAGVMILNRPKALNALTHDMVRQISAALTQW
                     ERDPAVTRVVIKGAGERAFCAGGDIRVVHDLGRAGRHAEALEFWRDEYVMNRQIKRYP
                     KPYIALIDGIVMGGGVGVSLHGSHRVAGERYNFAMPEVGIGFFPDVGGTYALPRLPFH
                     SGSYLALTGQRIGRADAIALGLATHAVAGSDFAALEAELIAGGPVDATLARFAAPAEP
                     APILEHRDEIAQIFALETVPAVLAALERLAPTSAYARATLATIRAKSPTSVALALEQM
                     LRGVSLSFEDAMTLEMRIVTRIIHGEDFYEGVRAAILDKDQAPRWQPDRLEAIDPAAI
                     QRYFAPLPDDLTFPAA"
     gene            3329..3793
                     /locus_tag="PLQ11_08005"
     CDS             3329..3793
                     /locus_tag="PLQ11_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24883.1"
                     /translation="MTDPVTSQPATALRASRITDAPIGSVREPVGVRWLMVLISFMRI
                     AALYWLAKGLLHWAYIIGLADPGFGDLRLSRQGLVMGLAVADLVAGVGLWMGASWGAA
                     VWIVVLFVEGSAPFLFPDMGFSMLDPVVALLAGALYLAFAWAARREQHAAYH"
     gene            4039..4551
                     /locus_tag="PLQ11_08010"
     CDS             4039..4551
                     /locus_tag="PLQ11_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HRK24884.1"
                     /translation="MNKVNSIAAQDVPSAKPTPVRGHYLQSLALVERLHRRLLDVIKD
                     ELDRRGRTDVNSVQALLLYNIGDNEVTAGELRTRGYYLGSNVSYNVKKLVEMGYLSHQ
                     RSRVDRRSVRIRLTKLGEEVRAMVGGLYEKHVMTVEQIGGISVDDFTRLNNALGRLDR
                     FWTDQIRYRL"
     gene            4900..6108
                     /locus_tag="PLQ11_08015"
     CDS             4900..6108
                     /locus_tag="PLQ11_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRK24885.1"
                     /translation="MPALAQRNSGAAGQAEWRQTYDASPLRDRPVKSAAPILSPQTIA
                     ATEAALVQFQQIVQRGGWQPLPNGIRLKLGAKSPVVVQLRGRLITGGDLDATNSNSPV
                     FDSYVEAGVKRFQERHGLGPTGVVGPQTIAAFNVPADVRLKQLEINLVRLKSYGQNLG
                     NRYAMANIPAAYVETVENNTVATRHVAGVGKVDRQSPIMQAKIYNVNFNPFWTVPASI
                     IRKDLIPKMQKDPNYLTEQKIRIYNKAGQELPPSQVNWNSYEATNYMFRQDPGGEFNS
                     LGVVRIDIANPHGVYMHDTPAKGIFGDDFRFVSSGCIRVQNVREFIAWILKDTPGWDR
                     DQIEAAIRSGNRIDAKPNAQIPVYWVYVTAWATPEGAIQFRDDIYGRDGFGPGAVTAA
                     IQAGDSEPEQ"
     gene            6290..6955
                     /locus_tag="PLQ11_08020"
     CDS             6290..6955
                     /locus_tag="PLQ11_08020"
                     /inference="COORDINATES: protein motif:HMM:NF014997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HRK24886.1"
                     /translation="MTLPADHPRLTRRPFSQAELIADGLLHAVALLASIIGLTVLIVL
                     VLFRRGNLELSAVVIYGVGLLAMFGFSAAYNLVPISPLKWVLRRFDHSSIYLMIAGTY
                     TPFLVLIGDPFWSIIMGIIVWGGAISGIVLKVALPGQFDRLSIAIYILLGGSALIAIR
                     PMVEKLPAPTLYLLLAGGIIYVAGVAFYVWKNLRFNNVIWHGFVSVAAGCHYAAIAYA
                     MTA"
     gene            complement(6964..>8704)
                     /locus_tag="PLQ11_08025"
     CDS             complement(6964..>8704)
                     /locus_tag="PLQ11_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRK24887.1"
                     /translation="PGTVLTETIDLDLKRPDGRTLPVRLFHSVAFAADGTRGPSRTLV
                     LNRSPGEDVAEGLRAAEVKFARFFNSTPLAIATVNKQGQVVRSNAPFARAFADQMARL
                     PPGENRSVIAVVSDEERAQITAALASAHEGKGEITPLDLTLAGDPPRSARFFISAVEE
                     RENEEESAIVYALETTEQQKLQEQFFQAQKMNAIGQLAGGVAHDFNNHLQSILGFADL
                     LLTNHKPSDASFADIVQIKNSANRAKSLVRQLLAFSRQQTLTPEEIHIGERLSELSNM
                     LKRSIGPKVELDVRYARDLWPVHADPASFDNMIINLAVNARDAMPNGGKLTIRTANIS
                     AAESRKFDSERLPEGEYALIEVEDTGTGMPPEVLEKIFEPFFTTKEVGRGTGLGLSSV
                     YGFVQQSGGVILCESTQGKGTAFRIYLPRFQPKAANAPQTVATGHGEAARMSTEPAKP
                     APAADLTGQGIILLVEDEEAVRNFAARALSSRGYTVLQAPSGVEGLAIAQGRIREIDL
                     VVSDVVMPEMDGPTMLGELRKLNPEIKVIFVSGYAEEAFRKNLPEGQQFNFLPKPFSL
                     KQLIETVKTVISE"
BASE COUNT         1544 a         2881 c         2704 g         1575 t
ORIGIN      
        1 caaagcccat gccgccatag tggaggctga ccagcgcccc cgccagcccg gccatcgccg
       61 ccgcaatggc aaagctctgc accagcacct cctgcggatc gatgccgaac agttcggctg
      121 ccaccgcatc atcggccacc gcatgccaat cgcgaccgaa ccggccacga cgcagcgcaa
      181 cggccatcgc cagcgccgaa ccgccggcca tgatcgccgt cagcagcgcc atcggcgtca
      241 gcgtcacgac aaagcccggc gcctcggcca ccacgatcgg cgccgccagc aggggcggca
      301 gccacagcgc gcttgccccc tgcgccagcc gcaggccttc gctcagcacc agcgacagcc
      361 cgacactggc gatcagcacg ccctgccccg gccggaacgt cagccgtccg atcaccagcc
      421 gcgccgccgt cagcccgtag agcgttgccg tcatccaggc gatccacagc ccgcccacca
      481 tgcccaccga cagcgatggc aggccgagaa ggtcgatcag catcatcgcc agcaccgcta
      541 ccgagccgcc cagcgcggca aaaccgccga aggcaaggtt gatccgcccc accagcccat
      601 agatcaccgc ataggaggcc gagatcagcg cgtagatgcc gagcttgggc agcgccgcca
      661 gcccctgttg cagaccatag gccacgccgg gcgaaagctg cgtcaccgtt ccgcgcggca
      721 gcgccggcgc atcggccacc gcatcctgcg aggtcagcca gcgttcgcgc aggaagaaca
      781 ccgccgaggc cgacaggctg gagccgttga ccgccacgcc cgtcagcacg cgccggtcca
      841 ggccaagtcc ggaaccggcg aaacggcagg ccagcaattg ttcggccgcc agccccggcg
      901 tccggaaccg gacaaagacg gtccccgcct cgcccgcctc ttcggcaacg attgtcgccg
      961 gccgctccgg cgccaccacc ggcagcacct gccggcacag cacgatctgc tcgctgtcga
     1021 ggccgagacc gcagccggcg agggccagag caaggcccag gaccagcccg atcacgatcc
     1081 cgccagatgg cagacgcaag gctcagtctt tggcgagcag ccgcgccacg gccggcgcga
     1141 aataggtgag aatgccgtcc gccccggccc gcttgaacgc gatcaggctc tccagcatcg
     1201 ccgcctcgcg atcgagccag ccgcgctcgg ctgcggccat gatcatcgcg tattcgcccg
     1261 acacctgata ggcgaaggtc ggcatgccaa agccctgctt cagccgaaag atgacatcga
     1321 gatacggcat gccgggcttg accatcacca tgtcggcgcc ctcggaaatg tcgagttcgg
     1381 cctccaccag cgcctcggcg ccgttggccg ggtccatctg gtaggttttc ttgtcgccgg
     1441 tcagcgtctt gttggccccc actgcatcgc ggaacggccc atagaaggcc gaggcgtatt
     1501 tggccgaata ggccatgatc gacaccttgt ggaaacccgc attgtcgagc gcggcgcgga
     1561 tcgcgccgat ccgcccgtcc atcatgtccg agggcgcgat gatgtcggcg ccggcagcgg
     1621 cctgcgtcac cgcctgctgg cacagcacgg caaccgtctc gtcgttgaga atgtcgccgt
     1681 cgcgcatcag cccgtcatgg ccatgcgagg tgtagggatc gagcgcgaca tcggtgatga
     1741 tgccgatatc cagccccttg gccctgatct gccggcaggc cgaacagacg aggttctgcg
     1801 ggttgagcgc ttcggatccg cgttcgtcgc gcaacttggg gtcggtatag ggaaacagcg
     1861 ccacggccgg gatccgcagc cgggcagcct cctccacctc cttgaccgcc agatcgatcg
     1921 acaggcggaa aacccccggc atcgaggcga ccggctcgcg ccggtcctcg ccggcgacga
     1981 tgaagatcgg ccagatgaga tcgttgacgc tcagcgcatg ttcgcgcacc agccggcgtt
     2041 tccagtcgga cttgcgattg cggcgcggcc ggcgcgcgat gtcgaggctg gcaggtgtga
     2101 accgttgcgg agagttcatg gcatgtccgg caaaaggcag cgttgatgtt ggtggtgtat
     2161 caggtcaggg gcgccggcaa aagccggaac agcagcggcc ggattgacgc ccggccagcc
     2221 cgccgctacc tcgcttgcag ggcacggctt gcgccggacg atacatgagg agacccatga
     2281 cgagcgaagc ggagatcctg atccgtcgtg aaggtgcggc cggcgtgatg atcctcaacc
     2341 gcccgaaggc actcaatgcg ttgacccatg acatggtcag gcagatttcc gccgccctga
     2401 cgcagtggga gcgcgatccg gcggtcaccc gcgtcgtgat caagggcgcc ggcgagcgcg
     2461 ccttctgcgc cggcggagat atccgtgtcg tgcatgatct cggccgggct ggccgccatg
     2521 ccgaggcgct ggaattctgg cgcgacgaat atgtgatgaa ccgccagatc aagcgctatc
     2581 ccaagcccta cattgcgctg atcgacggaa tcgtcatggg cggcggcgtt ggcgtgtcgc
     2641 tgcacggttc gcatcgcgtc gctggcgaga ggtataattt tgccatgcct gaggtcggca
     2701 tcggcttttt ccccgatgtc ggcggcacct atgcgctgcc caggctgccc ttccattccg
     2761 gcagctatct ggcgctgacc ggccagcgca tcggacgggc cgacgccatc gcgctcggcc
     2821 ttgccaccca tgcggttgcc ggcagcgact tcgccgcgct ggaggcggag ttgatcgccg
     2881 gtgggccggt cgatgccacc ctggcgcgct ttgctgcccc ggcagaacct gcgcctattc
     2941 ttgaacacag ggacgagatc gcccagatct tcgcgctcga gaccgtgccg gccgtgcttg
     3001 ccgcgcttga gcgcctggcc ccgaccagcg cctatgcccg cgcgacgctg gccaccattc
     3061 gcgccaaatc gccaacctcg gtcgcgctgg cgctggagca gatgttgcgc ggcgtctcgc
     3121 tgtcgttcga ggatgcgatg acgctggaaa tgcgcatcgt tacgcgcatc attcacggcg
     3181 aggatttcta cgagggtgtc cgcgccgcga tcctcgacaa ggatcaggct ccgcgctggc
     3241 aacccgaccg gctggaggcc atcgacccgg cggcgattca gcgctacttt gctcccctgc
     3301 ccgacgatct gacctttccg gcggcctgat gaccgaccct gtgaccagcc aacccgccac
     3361 cgcgctccgt gccagccgca tcaccgatgc gccgatcggc tcggtgcggg agccggtggg
     3421 cgttcgctgg ctgatggtcc tgatcagctt catgcggatt gccgcccttt actggctggc
     3481 aaagggactt ttgcactggg cctatatcat cggattggcg gaccccggct tcggcgatct
     3541 tcgcctgtcg cgccagggac tggtgatggg gcttgccgtt gccgatctgg tcgccggtgt
     3601 cggattgtgg atgggagcct cgtggggagc tgcggtgtgg attgtcgtgc tgttcgtcga
     3661 aggctcggca ccgttcctgt tcccggacat gggattttcg atgctcgatc cggtcgtcgc
     3721 cctgctggcc ggtgcgctgt atctcgcctt tgcctgggcg gcccgccgcg aacagcacgc
     3781 ggcctatcat tgagcaggcg acgttcgttc tcctatcctt aatgaaaaat ctcattgtaa
     3841 atgaatcttt tttgttgaga gtttggcaac attaccaatt ataccaagta aaaatcgatt
     3901 catattatca gataaatcaa atctttatcc tgtaatttaa actcgtcttc atcgggcaac
     3961 gctatggtca gtcccaagat ggcaaaagcc gtcaagaaca acgcccacaa agggcagcgt
     4021 caaacagggg acagagtcat gaataaagtc aattcgattg ccgcccagga tgtgccgtcg
     4081 gccaagccga caccggtgcg cggccattat cttcagtcgc tggcgctggt cgagcgtctg
     4141 catcgccgcc tgctcgatgt catcaaggac gaactggatc gtcgcggccg caccgatgtc
     4201 aacagcgtcc aggcgctgct gctctacaac atcggcgaca acgaggtgac cgcgggcgaa
     4261 ctgcggacac gcggctacta cctcggctcc aacgtatcct acaacgtcaa gaagctggtc
     4321 gagatgggct atctcagcca tcagcgctcg cgtgttgacc gccgctcggt ccgcattcgc
     4381 ctgaccaagc tgggcgagga agtgcgcgcc atggtcggcg gcctctatga gaagcacgtc
     4441 atgaccgtcg agcagatcgg cggcatctcg gtggatgatt tcacccgcct gaacaacgct
     4501 ttgggccgcc tcgaccggtt ctggaccgac cagatccgct atcgcctgta attcggacgg
     4561 cttcgccgtg gggacggcga ggctttcctg catcaacctc caaaactggc ggctcttcac
     4621 gcgagagccg ctttttttgt tgcccgagcc ctgcggcatt ttggccacag cccgccgcct
     4681 gttgccgatc acaccctcgt gaacgtcaca aattcgcctt acctccaaag gcatacgatc
     4741 gattaaccga atgtcgacca gccgctggca tggtgcgctc tggggcttgc cgagcggcca
     4801 tgtggaattg gagaaagtgc agtgacgcat cgtgagtttt tgaaccggcg tggatttctg
     4861 ctgggcacga ccgcagcgtt tggacttggg gcaacggcgg tgccggccct ggcccagcgc
     4921 aacagcggcg cggccggcca ggccgagtgg cgccagacct atgatgcgtc gcccttgcgg
     4981 gatcgcccgg tcaaatcggc ggctccgatc ctctcgccgc agacgattgc ggccaccgag
     5041 gcggcgctgg tgcagtttca gcagatcgtc cagcgcgggg gctggcagcc gttgcccaat
     5101 ggaatccgcc tcaagcttgg cgccaagagc ccggttgtcg tgcagttgcg cggccggctg
     5161 atcaccggcg gcgaccttga tgccaccaat tccaattcgc cggttttcga ttcttatgtc
     5221 gaggcgggcg tcaagcgctt ccaggaacgt catggcctcg gcccgaccgg cgttgtcggg
     5281 ccgcaaacca tcgccgcatt caatgtgccg gccgacgtgc gcctcaagca gctggagatc
     5341 aacctcgtcc ggctgaaatc ctatggtcag aacctcggca atcgctacgc gatggccaat
     5401 atcccggcgg cttacgtcga aacggtcgag aacaatacgg tggcgacgcg ccatgttgcc
     5461 ggcgtcggca aggtcgaccg gcagtcgccg atcatgcagg cgaagatcta caatgtgaac
     5521 ttcaacccgt tctggacggt tccggcctcg atcatccgca aggatctgat ccccaagatg
     5581 cagaaggacc cgaactacct gaccgagcag aagatccgca tctacaacaa ggccggccag
     5641 gaactgccgc ccagccaggt caactggaac agctacgaag cgaccaacta catgttccgc
     5701 caggacccgg gcggtgagtt caattcgctc ggcgtggtgc gcatcgacat cgccaatccg
     5761 cacggtgtct acatgcacga cactcccgcc aagggcatct tcggcgacga tttccgcttc
     5821 gtgtcctcgg gctgcatccg cgtgcagaac gtgcgcgagt tcatcgcctg gattctcaag
     5881 gacacgcccg gctgggatcg cgaccagatc gaggccgcca tccgctccgg caaccgtatc
     5941 gacgccaagc cgaacgcgca gatcccggtc tactgggtct atgtgaccgc ctgggcaacg
     6001 cccgagggcg ccatccagtt ccgcgatgac atctatggcc gcgatggctt cggccccggc
     6061 gcggtcacgg ctgccatcca ggccggcgac agcgagccgg agcaatagga ccaggcgacc
     6121 ataaaccctc aggagaggcc gacggttcag aaccaggggc caatggaagt cgacgggcag
     6181 gtctggcccc tgcggcaggt tgaaagcccg gtgttcagtt aaaactcggc gctttgcgcc
     6241 cgacgacaac ctgttctagc atccgccaga caaaatgtcg gatgccgcca tgaccctgcc
     6301 tgctgatcac ccgcgcctga cgcgccgtcc attcagccaa gccgagttga tcgccgatgg
     6361 cctgctgcat gccgtggcgc tgctcgcctc gatcatcggg ctgacggtgc tgatcgttct
     6421 cgtgctattc aggcgcggca acctcgaact ttcggccgta gtgatctacg gtgtggggct
     6481 tttggcgatg ttcggcttct cagccgcgta caatctggtg ccgatctcgc cgctgaaatg
     6541 ggtgctgcgt cggttcgatc attcctcgat ttatctcatg atcgcgggga cctacacgcc
     6601 gtttctggtg ctgattggcg atccgttctg gtcgatcatt atggggatta tcgtctgggg
     6661 cggcgcgatt tccgggattg tgctgaaagt ggcgctgccg ggacaattcg accggctgtc
     6721 gattgcaatt tacatcctgt tggggggctc ggcgctgatc gcgatccggc caatggtgga
     6781 gaaactgccg gctccgaccc tctatctcct tctcgcgggc ggcatcatct atgttgcggg
     6841 cgtggcgttc tatgtctgga agaacctgcg cttcaacaac gtcatctggc acggctttgt
     6901 atctgtcgct gccggatgcc actacgccgc gatcgcctat gccatgacag cgtgatctgc
     6961 accctactcg ctgatcaccg tcttgaccgt ctcgatgagc tgcttcagcg agaacggctt
     7021 gggcaggaag ttgaactgct ggccttccgg caggttcttg cggaaggctt cctcggcata
     7081 gccggagacg aagatcacct tgatctccgg gttgagcttc cgcaattcgc ccagcatcgt
     7141 cggcccatcc atctccggca tcacgacgtc ggagacgacg agatcgatct cgcggatcct
     7201 accctgcgcg atcgccagcc cttcgacacc ggagggcgcc tgcaacaccg tatagccgcg
     7261 cgacgacagt gcccgcgccg cgaaattgcg caccgcctcc tcatcttcga ccagcaggat
     7321 gattccctgc cccgtcaggt cggcagccgg ggccggtttg gccggctcgg tcgacatccg
     7381 cgccgcctcg ccgtggccgg tggcaacggt ctgcggcgca ttcgccgcct tcggctggaa
     7441 ccgtggcagg tagatgcgga aggccgtgcc cttgccctgg gtcgattcgc acaggatgac
     7501 gccgcccgac tgctgcacaa agccatagac cgatgacagg ccgaggccgg tgccgcgccc
     7561 gacctccttg gtggtgaaga acggctcgaa gatcttttca agaacctccg gcggcatgcc
     7621 tgtcccggta tcctccacct cgatcagcgc atattcgcct tcgggcaggc gctcggaatc
     7681 gaacttgcgg ctctcggcgg cggaaatatt ggcggtgcgg atggtcagct tgccgccatt
     7741 cggcatggca tcgcgcgcat tgaccgccag attgatgatc atgttgtcga acgaggccgg
     7801 atcggcatgg accggccaca gatcgcgcgc atagcgcaca tcgagttcga ccttcgggcc
     7861 gatcgaccgc ttgagcatgt tcgacaattc gctgagccgc tcaccgatgt ggatttcctc
     7921 aggcgtcagc gtctgctggc gcgagaaggc gagcagctgg cgcaccagcg atttggcccg
     7981 gttggcgctg ttcttgatct gcacgatgtc ggcgaacgag gcatcggacg gcttgtggtt
     8041 ggtcagaagc agatcggcaa agcccaggat cgattgcaga tggttgttga aatcgtgggc
     8101 gacaccgcca gccagctggc cgatggcatt catcttctgc gcctggaaga actgctcctg
     8161 cagcttctgc tgctcggtcg tctccagcgc atagacgatg gccgattcct cctcgttctc
     8221 gcgttcttcc accgcggaga tgaagaaccg ggcggaacgc ggcggatcgc cggccaatgt
     8281 caggtcgagc ggagtgattt cgcccttgcc ctcatgcgca ctcgccagcg cggcggtgat
     8341 ttgcgcccgc tcttcgtcgc tcaccacagc gatcaccgag cggttctcgc ccggtggcag
     8401 cctggccatc tggtcggcga aggcgcgggc gaacggcgcg ttggaccgca ccacctgccc
     8461 ctgcttgttg acggtggcga tggccagcgg agtcgaattg aaaaagcgcg cgaacttgac
     8521 ctcggccgcc cgcagcccct cggcgacatc ctcgcccggc gagcggttga gcaccagcgt
     8581 ccgggagggc ccgcgcgttc cgtccgccgc gaaggcgacg gaatggaaca gccgcaccgg
     8641 cagcgtgcgg ccatccggcc gcttgaggtc aaggtcaatg gtctcggtca gcacggtgcc
     8701 gggc
//
LOCUS       DAOMXG010000103         8657 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_4296_length_8657_cov_8.824576, whole genome shotgun sequence.
ACCESSION   DAOMXG010000103 DAOMXG010000000
VERSION     DAOMXG010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 8657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8657
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4296_length_8657_cov_8.824576"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..570
                     /locus_tag="PLQ11_08030"
     CDS             112..570
                     /locus_tag="PLQ11_08030"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012317099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HRK24888.1"
                     /translation="MSDHSGSNDFFDMAFAEARAAAKRGETPVGAVVVRDGLVLARAG
                     NRTREWSDPTAHAEVVAIRAACAGLGSERLPGADLYVTLEPCALCAAAISFARIRRLY
                     FAADDPKGGAVEHGPRLFSQPTCHHAPEVYGGLRASEAAELLKTFFLMRR"
     gene            complement(575..1432)
                     /locus_tag="PLQ11_08035"
     CDS             complement(575..1432)
                     /locus_tag="PLQ11_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRK24889.1"
                     /translation="MAKPPARRQRPRVALVLGGGGARGLAHIVVLETLETLGIRPVSI
                     AGTSIGAIIGACYAAGVPGRDLRRHALTIFRDHADVMARLFACRVGKLADLWSQGLGN
                     PLQVDAEQVLARFLPRPLPARFEDLSIPFRAIATDFYGLGRLEVGTGPLLPGVAASMA
                     LPWLVRPILREGRVLIDGGAVDPLPVRSADPTADLVLAVDVSGGVRGEAETRLPTPME
                     AMLGLSMIMQQALTEARLSVAPPNVRVLRPPVQDFRMLEFFSAKAILAAAEPLRGQIE
                     SLIAQAGQR"
     gene            complement(1435..2337)
                     /locus_tag="PLQ11_08040"
     CDS             complement(1435..2337)
                     /locus_tag="PLQ11_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRK24890.1"
                     /translation="MSDLPDLFPGFDSHYVPTEAGKIFVRTAGRGEPLVLLHGFPQTM
                     ACWHRIAPTLARRHRVVLIDLRGYGWSSAPQPEDETHDLYSKRAMAEDVLKVMEHLGH
                     IRFALAGHDRGARVGYRLALDHPGRVSKLALLDIIPTSTMWERMDASRAMQVYHWTFL
                     AQPRPLPENLIKRSPTEWLEHTLASWTASKDLKAFAPGALAHYRAFFQDPARITACCE
                     DYRAGASVDPEHDEADLKRGNTLVCPTLILWGRAGIPAAGTGPLDIWRSSFAPDAVGH
                     GIDGGHFLPEENPAATLEAFEEFF"
     gene            complement(2504..3874)
                     /locus_tag="PLQ11_08045"
     CDS             complement(2504..3874)
                     /locus_tag="PLQ11_08045"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HRK24891.1"
                     /translation="MARDTVDLQPLTRRDELVAWFEKGCKTEGPLRLGTEHEKFAFRP
                     GTLAPVPYDGADGIGALLTALQQRTGWEPIVENGAIIGLFDEAGGGAVSLEPGGQLEL
                     SGAPLETLHQTAAELDRHLADVHAIGARLDMRFLALGMTPKWRRSEVPVMPKSRYRIM
                     TAYMPKVGSLGLDMMYRTSTVQVNLDFTSEADMAMKMRVSLALQPLATALFANSPFTD
                     GKLNGLRSLRSEIWRDTDPNRSGMLPFVFEEGFGFERYVDYALDVPMYFIKRGDDYLD
                     ATGTSFRDLFAGRHPLLPGQPAVLSDWVNHLSTIFPEVRLKRYLEMRGADVGSRDHIV
                     ALSAFWVGLLYDRSALSAAWDLVSGWTAAERQDLRDAVPRQGLGATIRGRSLRDLGRE
                     VLALARAGLKARARHDGTGHDETRYLDLLDARIASGRVAADDWIAAFNGPWRGSVDPV
                     FSEAVI"
     gene            complement(3941..4711)
                     /locus_tag="PLQ11_08050"
     CDS             complement(3941..4711)
                     /locus_tag="PLQ11_08050"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012605866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HRK24892.1"
                     /translation="MASYDFSTHRLFVTADLGPGARFVLSHDQANYLMNVLRMAQGQR
                     FLAFNGRDGEWQVELSGGSRKAPEVLAVQQMRPQPDAGDLWYLFAPLKSARLDYMVQK
                     AVEMGASRLVPVMTHRTVAARVNLERMRANAVEAAEQCGILSVPDVADPIRIESLVGA
                     WQTDRILVWCDEDAPITNPLTALSSVQPGMPLALLIGPEGGFDAAEREKLLRLSNSLR
                     ISLGPRILRADTAAVAALALLQACCGDMAIDAVHKGRA"
     gene            4814..5254
                     /locus_tag="PLQ11_08055"
     CDS             4814..5254
                     /locus_tag="PLQ11_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24893.1"
                     /translation="MRAGRALMWLVVIGGAVGTVPAVAQQMPESCQKDFVPLMQKRQQ
                     FMATIEGYRKRKPTAAQACGTFRNLAVHNKKLADWMTAQKDWCQIPEDMLKGVTESQA
                     QIEQTRGSVCGAAAKQAAQIRKMQSQQPRQPAPGSGVRLPQGAL"
     gene            5251..6201
                     /gene="ubiA"
                     /locus_tag="PLQ11_08060"
     CDS             5251..6201
                     /gene="ubiA"
                     /locus_tag="PLQ11_08060"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020090669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HRK24894.1"
                     /translation="MTPPDGAVYDPKPADSVSTVLVEHVLPPRARPYARLARLDRPIG
                     WWLLLLPCWWSSALAAAAQASPPNPWHFCLFLVGSVVMRGAGSTWNDIVDRKLDAKVE
                     RTRNRPIPSGAVSTRQAALFMAALLLAGLCILLLFNRFAIVLGLAAMLPVVVYPFIKR
                     ISHHPQIVLGLAFAWGGLMGWAAMAGALAGPAYCLYAAAIAWTVGYDTIYALQDIEDD
                     PSVGIGSTALAYGEKVPLFVGAMYGLAVALAVLAVALAVPGGLLAWAGVAGFAIHLAM
                     QVKNIRLDQPAHCLKQFKSNRNAGLILTAGLVAEAVYRAI"
     gene            complement(6186..6632)
                     /locus_tag="PLQ11_08065"
     CDS             complement(6186..6632)
                     /locus_tag="PLQ11_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24895.1"
                     /translation="MTGPLAGLGPADFMCAGPDATPPQPANDNEPGVPAAGGFRGLLL
                     RQADAGYEILLCHSEPGLSQRVETVADDTEVIALWRSIGRKLNLVLFAETADGEIVQI
                     DPMPSLTALPRRFGNPVVSRRPRFARRRKMGNAAAASVTTGVQIAR"
     gene            6894..7715
                     /locus_tag="PLQ11_08070"
     CDS             6894..7715
                     /locus_tag="PLQ11_08070"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HRK24896.1"
                     /translation="MSDPLLPSSQLSRLRDILVRAAVAGGEAAMPFYRPAARTTATIG
                     YKASQSPVTEADYAADAAIRAALTEALPEAAIFSEEAAKGEARFSRRQVAVVDPIDGT
                     RAFIAGRSEWCVSVALLDDGRPVAGVIHAPARGETFAAAARLGATLNGRILSRPVPSG
                     GAMRITGPMHLIDQLDEHWPDLVGGETLKALAYRLVTVTDGRHDIAVASTGAHDWDIA
                     AAAVILAEAGCTLLTFSGERPVYNAAIPVHPALLAAEAGTAHRLAGLLAAAGSSA"
     gene            7740..7985
                     /locus_tag="PLQ11_08075"
     CDS             7740..7985
                     /locus_tag="PLQ11_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008550012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4170 domain-containing protein"
                     /protein_id="HRK24897.1"
                     /translation="MPTQSKNDQLLHLVFGGELVDLDRTEFKDLTKLDYVGMYPNFKA
                     AQAAWKAKAQQSVDNAQMRYFIVHLHRLLDPEADDHH"
     gene            8004..>8657
                     /locus_tag="PLQ11_08080"
     CDS             8004..>8657
                     /locus_tag="PLQ11_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRK24898.1"
                     /translation="MFKAIWRSPQVQEALGAALAAYLRFVKRTNRLILPTPDPYEAFA
                     GQEPAILAMWHGQHFMIPLGLREGQRFSVLISRHGDGELNAIAVRRLGIGLVRGSGAQ
                     RLDQVRKRGGVKALLDMLATLERGENMALTADVPKISRVCGRGIITLAQKSGRPILAV
                     AVVCSRRIDFARSWDRSSIGLPFGTTALVIGEPIRVAADAEAGQLERARLAVEAELDR
                     "
BASE COUNT         1548 a         2783 c         2848 g         1478 t
ORIGIN      
        1 agccgcgcga cttcggcggc cgagcgcggg ggccgcgctt gtgatcatca tcattgtctg
       61 tcatgccggc tccatagcag cgtggagagg ctggagcgag gaaataatcg gatgtctgat
      121 cacagcggca gcaacgattt cttcgatatg gcctttgccg aggcgcgggc agcggcgaag
      181 cgcggcgaga caccggttgg cgcggtcgtt gtacgcgacg ggctggtgct ggcgcgtgcc
      241 ggcaaccgaa cccgcgaatg gagcgatccc acagcccatg ccgaggtggt cgccatccgg
      301 gccgcctgcg ccggtcttgg cagcgagcgc ctgcctggcg ccgacctcta tgtcacgctg
      361 gagccctgcg cgctgtgcgc ggcagccatt tcctttgcgc ggattcgtcg attgtatttc
      421 gccgcagacg atccgaaagg cggagcggtg gagcatggcc cgcgcctctt ctcgcagcct
      481 acctgccacc atgccccgga agtttatggc ggtctgcgcg ccagcgaagc cgccgaactg
      541 ctgaagacat tcttcctcat gcggcggtga gcgttcagcg ctggcccgcc tgtgcgatga
      601 ggctctcgat ctgaccgcgc aatggttcgg cggcggcaag aatggccttg gccgagaaga
      661 attccagcat gcggaagtcc tgcaccggtg gccgaagcac ccgcacattc ggcggcgcca
      721 cgctcagccg cgcctcggtg agcgcctgct gcatgatcat cgacaggccc agcatggctt
      781 ccatcggcgt tggaagtcgc gtctccgcct cgccgcgcac accgccgctg acatccacgg
      841 ccaacacgag gtcggccgtc gggtcggccg agcgcaccgg caggggatcg accgcaccgc
      901 cgtcgatcag aacacgccct tcgcgcagga tcggccgcac cagccagggc aacgccatgg
      961 aagcggcaac acccggaagc aacggcccgg tgccgacttc cagccgaccc agcccgtaaa
     1021 agtcggttgc gatcgcccgg aagggaattg acagatcctc gaaccgggcc ggcaggggtc
     1081 ggggaagaaa ccgtgccagc acctgctcgg catcgacctg caacgggttg ccaagcccct
     1141 gggaccatag atcagccagt tttcccacac ggcaggcgaa cagccgcgcc attacatccg
     1201 catgatcacg gaaaatggtc agcgcgtggc gtcgcagatc cctgcctggc acgccagcgg
     1261 catagcaggc gccgatgatt gcgccgatcg aggttccggc gatcgacacc ggacgaatgc
     1321 cgagcgtttc gagcgtctcc aggacgacga tatgggcaag accgcgtgcg ccgcctccgc
     1381 ccagaaccag tgccacgcgt ggcctctgcc tgcgcgccgg cggctttgcc atggtcagaa
     1441 gaactcctcg aacgcttcca gcgtcgcggc cgggttctct tccggcagga aatgcccacc
     1501 gtcgataccg tgaccaaccg catccggggc gaagctggag cgccagatgt cgagcggccc
     1561 ggtcccggca gcgggaatgc cggccctgcc ccacagaatc aatgtcgggc agacaagcgt
     1621 gttgccacgc ttgagatccg cctcatcatg ctccggatcg accgaagcgc cagcacgata
     1681 atcctcgcag caggcggtga tgcgtgccgg atcctgaaaa aaggcacgat aatgggcaag
     1741 tgctccggga gcgaaggctt tcaggtcctt cgaggccgtc cagctcgcca gcgtatgctc
     1801 cagccattcg gtcggtgaac gcttgatcag gttttccggc aagggccgcg gctgggcaag
     1861 gaatgtccaa tgatagacct gcatggcgcg cgaggcgtcc atccgctccc acatggtcga
     1921 cgtggggatg atatcgagca gcgcgagctt gctgacacgg cccggatgat cgagcgccag
     1981 ccggtagccg acgcgggcac cgcgatcatg gccggcaagc gcaaagcgga tatgaccaag
     2041 atgctccatc accttgagaa catcctcggc catggcgcgc ttggagtaga gatcatgcgt
     2101 ttcgtcctcg ggttgcggcg ccgaggacca gccgtaaccg cgcagatcga tcagcacgac
     2161 gcgatgccgg cgggccagcg tcggggcgat gcggtgccag caggccatgg tctgcgggaa
     2221 accgtgcagc agcaccagcg gttcaccccg gccagcggtg cgcacaaaga tttttccggc
     2281 ctcggtcggc acatagtgcg aatcaaatcc ggggaagaga tcgggcagat cggacatatc
     2341 gcgaaatctc cggaatcgct gcgccttgaa cgaggcggcg accttcccac aaaccgcgca
     2401 gcgatgccat cggctttacg tcacatccgc aggctagagt attttccgca ggatttggcc
     2461 cgaaaatggt catcgcaatg attcaatctg gtcggaaagt gcgttaaatc accgcttcgc
     2521 tgaagaccgg atcgaccgaa ccgcgccatg gcccgttgaa agcggcgatc caatcgtcgg
     2581 cagccacccg gcccgaggcg atgcgcgcat caagcagatc aaggtatcgg gtttcgtcgt
     2641 ggcccgtgcc atcgtggcgg gcgcgcgcct tcagaccggc tcgcgcgagc gccagcacct
     2701 cgcggcccag atcgcgcaaa ctgcgaccgc ggatggtggc accaagcccc tgacgcggca
     2761 ctgcatcgcg caggtcctgt cgctccgccg cggtccagcc gctgaccaga tcccaggccg
     2821 ccgagagcgc cgatcgatca tagagcagcc caacccagaa ggccgagagc gcaacgatgt
     2881 gatcgcgcga gccgacatcg gcgccgcgca tctcgagata gcgcttgagc cgcacctcgg
     2941 ggaaaatcgt cgacaggtgg ttgacccagt cggacaacac cgccggctgg cccggcagca
     3001 gcggatggcg gccggcaaac aggtcgcgga atgaagtccc ggtcgcgtcg agataatcat
     3061 cgccgcgctt gatgaaatac atcggcacat caagcgcata atcgacatag cgctcaaagc
     3121 cgaagccttc ctcgaacacg aagggcagca tgcctgagcg gttgggatcg gtgtcgcgcc
     3181 agatttcgga gcgcaacgag cgcaggccgt tgagtttgcc gtcggtgaag ggcgaattgg
     3241 caaacagcgc ggttgccagc ggttgcagcg ccagcgaaac gcgcattttc atcgccatgt
     3301 ccgcttccga ggtgaaatcg agattcacct gcacggtcga ggtgcgatac atcatgtcga
     3361 ggccgaggct gcccaccttc ggcatatagg cggtcatgat gcgataccgc gacttgggca
     3421 tgacaggcac ttcggaacgc cgccacttcg gcgtcatgcc gagcgcaaga aagcgcatgt
     3481 ccagccgtgc gccaatggca tggacatcgg cgaggtgacg atcgagttcg gcggcggtct
     3541 ggtgcaacgt ttccagcggc gcgccggaca gttcaagctg cccgcccggc tccagcgaca
     3601 ccgcgccacc gccagcctca tcgaacagac cgatgatggc gccgttttcg acgatcggtt
     3661 cccagccggt gcgctgctgc agcgccgtca gcagcgcccc gataccgtca gcgccgtcat
     3721 agggcactgg agcgagcgtg ccggggcgaa aggcaaactt ctcgtgttcg gtgccaagcc
     3781 gcagcggccc ctcagtcttg cagcccttct cgaaccaggc gacgagttcg tctcgccggg
     3841 tgagtggctg aagatcgacc gtgtcgcgcg ccatgacgcc tccgtcgtgt tgccggatca
     3901 aaggaccgcg ggcggattgc tgaccggcgc gggtcggctt tcatgcccgg cctttgtgca
     3961 ctgcgtcaat tgccatgtcg ccacagcagg cctgcagcag cgccagggcc gcgacggcgg
     4021 cggtgtcggc gcgcaggatg cggggcccga gagaaatgcg caagctattg gaaagtctca
     4081 gcagtttttc tcgctcagca gcgtcaaagc cgccttccgg gccgatcagg agggccagag
     4141 gcatgcccgg ttgcaccgat gacaaggccg tgagcggatt ggtgatcggg gcgtcctcgt
     4201 cgcaccagac aagaattcgg tccgtctgcc atgcgccaac gaggctctct atccggatcg
     4261 gatcggcaac atcaggcacg gacagaatgc cgcattgctc ggcggcttcg acggcattgg
     4321 cgcgcatccg ctcgagattg acgcgggcgg cgactgtccg atgagtcatg accggcacca
     4381 ggcggctggc gcccatttcc accgccttct gcaccatgta atccagtcgt gcggatttga
     4441 gcggggcgaa aagataccaa aggtcgccgg cgtctggctg cggccgcatc tgctgcacag
     4501 ccagcacttc aggcgccttg cgcgagccgc cggacagttc cacctgccat tcgccgtcgc
     4561 ggccattgaa ggcgagaaag cgctggccct gcgccatccg cagcacgttc atcaggtagt
     4621 ttgcctgatc atgcgacaga acaaagcgcg cgccgggacc gagatccgcc gttacaaaaa
     4681 ggcgatgggt ggaaaagtca taactcgcca tggtatcgct cgataaaggc ctgtgcagat
     4741 ttggtcttga ttctgccagg gttcccgcgt tgaaagatgg tccgggttga gcggcaaggc
     4801 aacggagtga atgatgcggg ctggacgggc gctgatgtgg ctggtcgtga tcggcggagc
     4861 ggtggggacc gttccggcgg ttgcccagca gatgccggaa agctgccaga aggatttcgt
     4921 gccgctgatg cagaagcggc agcagttcat ggccacgatc gaaggttatc gcaagcgcaa
     4981 gccgacagcg gcgcaggctt gcggcacctt ccgcaatctt gctgtccata acaagaagct
     5041 tgccgactgg atgaccgcgc agaaggattg gtgtcagatc cccgaagaca tgctcaaggg
     5101 cgtgaccgaa tcacaggccc agatcgagca gacgcgcggc agcgtctgcg gcgcggccgc
     5161 caaacaggct gcgcagatcc gcaagatgca gagccagcag ccgcgccagc cagcgcccgg
     5221 gtcgggcgtg cggttgccgc agggggccct gtgacgccgc cagatggcgc ggtttacgat
     5281 cccaaacccg ccgattcggt cagcacggtc cttgttgaac atgtcctgcc gccgcgggcc
     5341 cggccctatg cgagacttgc ccgcctggac cggccgatcg gctggtggct gctgctgctg
     5401 ccgtgctggt ggtcgagcgc cttggcggcg gcggcgcagg ccagcccccc aaatccctgg
     5461 catttctgcc tgttcctggt cggctcggtg gtgatgcgcg gcgcggggtc gacctggaac
     5521 gacatcgtcg accgcaagct cgatgcaaag gtcgaacgga cccgcaaccg tccaattccc
     5581 tccggggcgg tctccacccg gcaagccgct ttgttcatgg ctgccctgct gcttgccggg
     5641 ctgtgcatcc tgctgctgtt caaccgcttt gccatcgtgc tgggccttgc ggcgatgctg
     5701 ccggtagtcg tctatccgtt catcaagcgg atctcgcatc atccgcagat cgtcctgggg
     5761 ctggcctttg catggggagg acttatgggc tgggcggcga tggcaggggc gctggcaggg
     5821 ccggcctatt gtctttatgc agcggcgatc gcctggaccg tcggctacga tacgatctac
     5881 gccttgcagg atatcgagga tgatccgtcg gtcgggatcg gctcgaccgc gctcgcctat
     5941 ggcgagaagg tgccgctgtt cgtcggcgcg atgtatggtc tggcagtggc gcttgcggtg
     6001 ctggcggtgg cgcttgccgt gccgggcggg ttgctggcct gggcgggcgt ggccggtttt
     6061 gccatccatc tggcgatgca ggtcaagaac atccggctcg atcagccggc ccattgcctg
     6121 aagcagttca agtccaatcg caacgccggg ctgatcctga ccgccggact ggtcgccgaa
     6181 gcggtctatc gagcgatctg aacaccggtt gttacagatg ccgccgctgc gttccccatc
     6241 ttgcgacgcc gggcgaagcg cggccggcgg ctgacgaccg gattgccgaa ccggcgcggc
     6301 agggccgtca ggctgggcat cggatcgatc tgcacgatct cgccatcggc tgtttcggca
     6361 aacagcacca gattgagctt gcggccgatc gaccgccaga gcgcgatgac ctcggtatcg
     6421 tcggcaaccg tctcgacccg ctgcgacaga ccgggttcgg aatggcagag caggatctcg
     6481 taaccggcat ccgcctggcg cagcagcagg ccgcgaaagc cgccggcggc gggcacgccg
     6541 ggctcgttgt cattggcagg ctgcggtggt gtggcgtccg gtccggcgca catgaaatcc
     6601 gccggaccga gccctgctag aggacctgtc atggttgatg ccttttccct gccgggtcgc
     6661 ttctgtggca atcccgttgt tgcaggcatc atgcggtgcg gacgccgcat atcggcttaa
     6721 aaatgctggt taagaggccg tttctggcca tgttatgcgc ggaaatgctg cggatgcttg
     6781 aagaacggtt agcaaattgc cgcgactgtc gaccgatccg cgcccctgtc gcaggggaac
     6841 cttctcccca aggagcgctt gatttaggtg ccaaacctgc caagacaccg gtgatgtccg
     6901 accctctcct gccctcatcc caactttccc gactgcgcga tattctggtg cgcgccgctg
     6961 tggccggtgg cgaggcggcc atgccgttct accggccggc cgccaggacg accgctacga
     7021 tcggctacaa ggccagccag tcgccggtga ccgaagccga ctatgcggcc gatgcggcaa
     7081 tcagggcggc gctgaccgag gcgctgccgg aagcggcgat cttttccgag gaggcggcca
     7141 agggcgaggc gcggttttcg cgccggcagg tcgcggtggt cgatccgatc gacggcaccc
     7201 gggctttcat cgccgggcgc agcgaatggt gcgtttcggt ggcgctgctg gatgatggca
     7261 ggccagttgc cggcgtcatt catgccccgg cgcgcggcga gacctttgct gcggcggcca
     7321 ggctcggcgc cacgctgaac ggcagaatcc tcagccgtcc ggtgccgtcc ggcggcgcca
     7381 tgcggatcac cgggccgatg cacctcatcg accaactcga cgagcactgg cccgatcttg
     7441 tcggcggcga gactctgaag gccctggcct accggctggt caccgtgacc gacggtcgcc
     7501 atgacatcgc ggttgccagc accggcgcgc atgactggga catcgcggca gcggcggtca
     7561 ttctcgccga agcgggctgc acgctgttga cgttttccgg cgaacggccg gtttacaatg
     7621 ccgccattcc ggtgcatcca gcgctgctgg cggccgaggc gggcaccgca catcgccttg
     7681 ccggcctgct ggctgcagcc ggatcatcgg cctgatcaat gtgacgtgaa ggacagttca
     7741 tgcccaccca atccaagaac gaccaacttc tccatctcgt gttcggcggt gaactcgtcg
     7801 atctcgaccg caccgaattc aaggacctga ccaagcttga ctatgtgggc atgtatccca
     7861 acttcaaggc cgcgcaggcc gcctggaagg ccaaggcgca gcaaagtgtc gacaacgccc
     7921 agatgcgcta tttcatcgtt catctgcatc gcctgctcga tcccgaagcc gacgatcatc
     7981 actgaccctt ccggcgaggc ttcgtgttca aggcgatctg gcgatccccg caggttcagg
     8041 aagcgctcgg cgcagcgctg gctgcatacc ttcgcttcgt caaacgcacc aacaggctga
     8101 tcctgccgac gcccgatccc tatgaagcct ttgccggcca agagccggcc attctggcca
     8161 tgtggcacgg ccagcatttc atgatcccgc tcggcctgcg cgaggggcag cggttctccg
     8221 tgctcatctc gcggcatggc gacggcgaac tcaacgccat cgccgtccgc aggctgggca
     8281 tcggcctggt gcgcggctcc ggcgcccaac ggctggatca ggtgcgcaag cggggcggcg
     8341 tcaaggccct gctcgacatg ctggcgacgc tcgagcgggg cgagaacatg gcgttgaccg
     8401 ccgatgttcc gaagatctcg cgcgtctgcg gccgcggcat catcacactg gcgcagaaat
     8461 ccggccggcc gatcctggcc gtggctgtcg tctgttcacg ccggatcgat tttgccagga
     8521 gctgggatcg gtcgagcatc gggctgccgt tcggcaccac ggcgctggtg atcggcgagc
     8581 cgatcagggt ggcggccgac gcggaggctg gccagctcga acgggcccgc ctggcggtcg
     8641 aggccgaact cgaccgg
//
LOCUS       DAOMXG010000104         8603 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_4316_length_8603_cov_8.810365, whole genome shotgun sequence.
ACCESSION   DAOMXG010000104 DAOMXG010000000
VERSION     DAOMXG010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 8603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8603
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4316_length_8603_cov_8.810365"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            364..448
                     /locus_tag="PLQ11_08085"
     tRNA            364..448
                     /locus_tag="PLQ11_08085"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:398..400,aa:Leu,seq:tag)
     gene            529..2007
                     /gene="tig"
                     /locus_tag="PLQ11_08090"
     CDS             529..2007
                     /gene="tig"
                     /locus_tag="PLQ11_08090"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018427703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HRK24899.1"
                     /translation="MQLTQTQSDGLKRQFQVTVPASELESRLNAEIEQLKTKVRINGF
                     RPGKVPAGHLKRLYGKSVMADVVQNAVTEANRRIVEENDLKLAMEPQIDFPEDKELVD
                     RMFDGKADLSFGVHVEVLPRIEVQDHSDIAVTRQVVAIEDKEVDAALERMASQNRSFN
                     DKGNKKAETGDRVTIDFVGTLDGVAFDGGSSEDADLVLGSGSFIPGFEDQLVGAKAGD
                     RKTVTVTFPEGYQAAHLAGKPAEFAVTVKAVAAPGDLVIDDELARKFGMEDLGKLKDA
                     IRRSLGEELNEIARSKTKRQLLDALDAKYAFDLPPTLVEQEFNTVWSQVQQDMQARKT
                     SFDAEGTTEDEARAEYRRIAERRVRLGLVLAELGSRAKVEVTDEEVTQALVTRARQFP
                     GREKQVWEFYQKNPQALAEVRAPIFEEKVIDTLLAAVTVTDQSVTREELLKDDEDEDK
                     PAKSAGKSAEKSKKAAAKAEAGEAAEKPKKAPAKKKVEKSES"
     gene            2113..2601
                     /locus_tag="PLQ11_08095"
     CDS             2113..2601
                     /locus_tag="PLQ11_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24900.1"
                     /translation="MAFVFSYIPGQTAGTLISGNGEFIVRVPFAFDDETGDVYSIVGD
                     FAVFPGGGELEFEFGFKVIRASPEFEQIDEIFWDGQATMPFLSDRGHRAMVLGELFEI
                     LRFLIVKIRPQRLVMMTHTANLPKRALVKFQKLVAVMREEGLDARKTDSWNGRHIWIA
                     IR"
     gene            2641..2823
                     /locus_tag="PLQ11_08100"
     CDS             2641..2823
                     /locus_tag="PLQ11_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24901.1"
                     /translation="MATLPISREKQIELMRTAAERQEARTRVWKSDPLVKRVMSEPAP
                     GQEKTAYQRVPLKKLG"
     gene            complement(2887..3363)
                     /locus_tag="PLQ11_08105"
     CDS             complement(2887..3363)
                     /locus_tag="PLQ11_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRK24902.1"
                     /translation="MNRESRTDAGAALTGLILDLFALSNRLLAAGDRLVADLGLTSAR
                     WQVLGTILSRDAPQPVAWLARDMGANRQNIQRIVNDLAAEGMVDFRPNPHHRRAQLVV
                     ITAKGRSVFDAAMQLQAPWINRLSQGLTAPELKKAHQIILALQAALLGEDDVSGLP"
     gene            3453..3824
                     /locus_tag="PLQ11_08110"
     CDS             3453..3824
                     /locus_tag="PLQ11_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011652662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HRK24903.1"
                     /translation="MSLLRPMDPQFPIEQQLAIAASPVVLINLFTLDEADEAVFLEVW
                     ADDAAFMKRQPGFISTQLHRAIGPRPTYLNYAVWQSTAEFRAAFSHPEFLSKLSAYPS
                     SSVATPHLFRKVAVPGLCVAG"
     gene            3836..4276
                     /locus_tag="PLQ11_08115"
     CDS             3836..4276
                     /locus_tag="PLQ11_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24904.1"
                     /translation="MLKRIHPVAGAIALLVIATFWISTVASELFGSPETVAAVKLAIP
                     WGFLLLVPALVATGGSGMKLAAGRRGGPLGKKMKRMPLIAANGLLVLIPAALFLAAKA
                     RAGVFDTSFYAVQTLELVAGAVNLVLLWRNFQDGLRLSGRRRRV"
     gene            4587..5606
                     /locus_tag="PLQ11_08120"
     CDS             4587..5606
                     /locus_tag="PLQ11_08120"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRK24905.1"
                     /translation="MNLKHWLGAAALTIGMVAGAGAASAQTAIKFTLDWVFQGPTSPF
                     LVALDKGYYKAEGLDVTMDPGQGSAGAVQRVATGAYQIGFADVNSLIEYNAKNPGKEV
                     LSVFMAYDFPPFGVYALKKSGIRTLKDLEGKKLGAPVFDASFRLFPAFAKIVGIDRAR
                     VESVNLTPQLREQSLVQGSVDFISGHYFSSMLDLKARGVKEDDVVKFMYSDHGMNVYG
                     NGIIIAPELAQNEKLVQGFIRATVKAWREVAANPSIGVAAVKKRDPLADEKLEAERLD
                     ISLKSNVLTPYVKANGMGDVDPERFGKALKEVADAFGLPAAPAADKVFTNRYLPPRAE
                     RMVAP"
     gene            5611..6414
                     /locus_tag="PLQ11_08125"
     CDS             5611..6414
                     /locus_tag="PLQ11_08125"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007196380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRK24906.1"
                     /translation="MTIAGTEPPLVEMRAVSLAYGRGKDTTLALADATLSIDKGEFIA
                     VVGPSGCGKSTLMKLVTGLLPPTGGEILVHGQKVTRPVRGVGMAFQNPTLMPWRTTRD
                     NILLPLEVVEPHKQRFRANKAEYVARVEALLTSVGLGGFGDKYPWQLSGGMQQRSNLC
                     RALVHEPEILMLDEPFGALDAFTREDLWGVMQALWMERKFTAVLVTHDLREATYLADT
                     IYVMSKRPGRVIVRKPIDIPRPRTLETTFEPHFVDIVHELRDLIRQEYA"
     gene            6617..7393
                     /locus_tag="PLQ11_08130"
     CDS             6617..7393
                     /locus_tag="PLQ11_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009452041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRK24907.1"
                     /translation="MNDSQRHLLKLAMPWLVMAVLLLIWELACILLAVPEIILPRPTR
                     IFEVFVQRFDILLRFCLETLWTTVAGFGLAIVFGLLLGLAIGASPFVYSGLYPLLIAF
                     NAIPKVAIVPILMIWLGVGATPAIITAFVISFFPIVVNVATGLATIEPEMRDVMRSLG
                     ATQYEILTKVGIPRAMPYLFASLKVAITLAFIGSVISETVGGNRGIGFLMLSAGARND
                     APTTFAGLFAIAIMGVAMYALCALIEKRMTRWAFRGEMAV"
     gene            7400..8041
                     /locus_tag="PLQ11_08135"
     CDS             7400..8041
                     /locus_tag="PLQ11_08135"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003500910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRK24908.1"
                     /translation="MTAAHPDARFWDRFARRYAAASIRDTAGYERTLARTSELIRGMA
                     SVLEIGCGTGTTALRMAGGVQHLLATDIAPQMIAIAREKVAAQGCSNVAFEVAAPDAI
                     TAPDGGFDAILAFNVLHLLRDDAATLAHLHTLLKPGGLLISKTPCLVELNLFIRLAIP
                     VMRRIGLAPYVRVQDAATLESRIAAAGFTIIEQARHGSTGKDFRTFLVARRPA"
     gene            8054..>8603
                     /locus_tag="PLQ11_08140"
     CDS             8054..>8603
                     /locus_tag="PLQ11_08140"
                     /inference="COORDINATES: protein motif:HMM:NF015551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HRK24909.1"
                     /translation="MGRFFSADFSAQAGVQGLIVALVGYASSAAIVIAGLKAMGAGTA
                     EIASGLLLLGLGKGVAAIGLSLWTRMPISIAWTTPGLALLATSPLPAGGFGTAVGAFI
                     ITGLLFVLAGLWPLLGRLVAAIPHPIASAMLAGILLKLCLAPFVALQALPLPALAIIA
                     TWLLVQRFARLYAVPAAVLVTPC"
BASE COUNT         1485 a         2639 c         2789 g         1690 t
ORIGIN      
        1 aggcgctgtc cggcgcgccg ggccgcatcg gccggaagcc gggcgtcagc ggcaaggcca
       61 gggtcagcgt cggcgccaga atcggctggg tccgggcggc ggatgcaaca atggttgcag
      121 cggcgctggc aatcagcgtg cggcggctgg gattgggtcg gattggcatg cgagggggtc
      181 ccggaagcgc gatgtcccag atagcggcca gcggcgcgat cgtgcaaggg gcaggggatc
      241 atcgcactgc agtcgagttt tttccgatgc cggacttttt ttgatgcagg acgctgcatc
      301 ccttgttata ggggccgctc gcttgccggc aggcgggcgc gcgagccttt cttcgttcgt
      361 gaagcgggcg tggcggaatt ggtagacgca ccggatttag gttccggcgg cgaaagctgt
      421 gggggttcga gtccctccgc ccgcaccacg ccgcgacgat cgaggccgtt ggcacaccaa
      481 tgcctgccgc aattgataca ggtttcaacg gcttatccgg atcgcaccat gcagcttacc
      541 caaacccagt cggacggcct caagcgccag ttccaggtca ctgtccccgc atccgagctg
      601 gagtcccggc tcaatgccga gatcgagcag ctcaagacca aggtgcgcat caatggtttc
      661 cgcccgggca aggtgccggc gggccatctc aagcggcttt acggcaaatc ggtgatggcc
      721 gatgtggtgc agaatgccgt caccgaggcc aatcgccgca tcgtcgagga gaacgatctc
      781 aagctggcga tggaaccgca gatcgatttt cccgaggaca aggagcttgt cgatcgcatg
      841 ttcgacggca aggccgatct gagcttcggc gtgcatgtcg aggtgcttcc caggatcgag
      901 gtgcaggatc attccgacat cgcggtgacc cgtcaggtcg tcgcgatcga ggacaaggag
      961 gtcgatgccg cgctggagcg catggcctcg cagaaccgca gcttcaatga caagggtaac
     1021 aagaaggccg agaccggcga ccgcgttacg atcgactttg tcggcacgct ggatggcgtt
     1081 gccttcgacg gcggcagttc cgaggatgcc gatctggtgc ttggttcggg cagcttcatc
     1141 cccggcttcg aggaccagct tgtcggcgcc aaggccggcg acaggaagac cgtgaccgtc
     1201 accttccccg agggctatca ggcggcccat ctggccggca agccggcgga atttgccgtc
     1261 accgtcaagg ctgtggccgc gccgggcgat ctggtcatcg acgacgaact cgccaggaag
     1321 ttcggcatgg aggacctcgg caagctcaag gacgccatcc gccgctcgct gggcgaggaa
     1381 ctgaacgaaa tcgcccgctc caagaccaag cgccagttgc tcgacgcgct tgatgccaaa
     1441 tatgcgttcg acctgccgcc gacgctggtc gagcaggagt tcaataccgt ctggtcgcag
     1501 gtccagcagg acatgcaggc ccgcaagacc agcttcgacg ccgaaggcac gaccgaggac
     1561 gaggcgcggg ccgagtatcg ccgcatcgcc gagcgtcggg ttcgcctcgg tctggtgctg
     1621 gccgagcttg gctcgcgcgc caaggttgaa gtgaccgacg aggaagtgac gcaggccctg
     1681 gtgacgcgcg cccgccagtt ccccggtcgt gagaagcagg tgtgggaatt ctaccagaag
     1741 aacccgcagg cgctggccga ggtgcgtgcg ccgatcttcg aggagaaggt gatcgacacg
     1801 ctgctcgccg ccgtcacggt gaccgaccag agcgtgacgc gcgaggaact gctcaaggac
     1861 gacgaggacg aggacaagcc ggccaagagt gccggcaaga gtgccgaaaa gtccaagaag
     1921 gcggctgcca aggcagaagc tggcgaggcg gccgaaaagc cgaagaaggc tccggccaag
     1981 aagaaggtcg aaaagtccga gtcctgatgt ccggatcgca ctgacaatgc atcaggcccc
     2041 gcgccgcccg cgcggggttg gctttttctg caatgccatg caagtctcga tactctgaac
     2101 ttgcggtgat tcatggcatt tgtattttca tatattcccg gacagactgc cggcactttg
     2161 atctctggca atggcgaatt tatcgtacgt gtgcctttcg ccttcgacga cgagactgga
     2221 gatgtctatt cgatagtcgg ggattttgcg gttttccctg gcggtggtga gcttgaattt
     2281 gaatttggct tcaaggtgat cagggcttca ccagaattcg agcaaatcga tgagatcttt
     2341 tgggacggac aagcgacaat gccgttcctg tcggatcgtg gtcacagggc catggttctt
     2401 ggcgaattgt ttgaaattct tcggtttttg atcgtcaaaa tacgaccaca gagattggta
     2461 atgatgaccc acactgctaa tttgcccaag cgcgcattgg tcaaatttca aaagcttgtt
     2521 gccgtgatgc gcgaagaagg gctggacgcg aggaaaaccg actcgtggaa tggccggcac
     2581 atttggattg cgatccgtta acacccacaa tatatattac tctcagatgg agtaaccgcc
     2641 atggcaacgc tgccgatttc gcgcgaaaag cagatcgaac tcatgaggac cgctgctgag
     2701 cgtcaggagg cgcggacgcg cgtctggaag tccgatccgc tggtcaagcg cgtcatgagt
     2761 gagccggccc ctgggcagga gaaaaccgcg taccagcgtg tgccgctcaa gaagcttggc
     2821 tgacctcacc ttcgtttgac tgaaggtttt gccccgcgcc gccagcgcgg ggctttttgt
     2881 tgggtttcag ggtaagccag agacatcatc ctcgccgagc aacgccgcct gcaacgccag
     2941 aatgatctga tgtgcttttt tcagctccgg cgcggtcaat ccctgtgaaa ggcggttgat
     3001 ccaaggcgcc tgcaactgca tcgcagcgtc gaaaacgcta cgtcctttcg cggtgatgac
     3061 cacaagctgg gcgcgccggt ggtggggatt gggccggaag tccaccatcc cctcggccgc
     3121 caggtcgttg acaatgcgct ggatgttctg gcggttcgct cccatgtcgc gtgcaagcca
     3181 ggccaccggc tgtggcgcat ccctggagag gatggtgccc aagacctgcc accgcgcgct
     3241 ggtcagcccc agatcggcca cgagccggtc gcccgctgcc agcaggcggt tgctcagggc
     3301 aaaaaggtcc agaatcagcc ccgtcagggc cgcgccggcg tcggtgcggg attcgcggtt
     3361 cattgcatca tccatctaaa ttgacaacat agtgtcaatt tgcaaaaatc gaccagatga
     3421 aggttgaata cccaaactgg aggttggttg ccatgtctct cttgcgtccg atggatccgc
     3481 agttcccgat cgagcagcaa ctggccatcg cggcctctcc ggttgtcctg atcaatctct
     3541 tcacgcttga tgaggccgac gaggctgttt ttcttgaggt ctgggccgat gatgcggcct
     3601 tcatgaagcg gcagcccggc ttcatctcca cccagttgca ccgcgccatc ggtccgcgcc
     3661 cgacctatct caactatgcc gtatggcaat ccacggccga gttccgcgcg gcgttcagcc
     3721 atcccgagtt tttgtccaag ctatcggcct atccgtcatc ctctgtggcg acgccgcatc
     3781 tgttccggaa agtcgctgtt ccgggcctct gcgtcgccgg ctgaaggagg aagggatgct
     3841 gaagcggatc catcccgtcg ccggcgcgat cgcactgctg gtcatcgcca cattctggat
     3901 ttcgactgtt gccagtgaac tgttcggctc tcctgaaacc gtggcagcgg tcaagctggc
     3961 cattccttgg ggctttttgc ttcttgtgcc ggctcttgtc gccaccggcg gttcaggcat
     4021 gaaactcgct gccgggcggc gcggcgggcc tttgggcaag aaaatgaagc gcatgccgct
     4081 gattgcggcc aacggtctgc tcgtgctgat ccctgccgcc ctgttcctgg cggcaaaagc
     4141 tcgcgccggc gtattcgaca cgagctttta cgcggttcag acgctggagc ttgttgccgg
     4201 cgcggtgaac ctcgttttgc tctggcgcaa ttttcaggat gggcttcgct tgtccggcag
     4261 gcgccgccgc gtctgatcga ccggcgatcg tggagcagct gggatcaccc cgcgccgccc
     4321 gcgcggggtt tttgttggcc tgaaatctgt gggcagcggc tgccaatggc cttgctttgc
     4381 ctgcccctgc tgggcgaacc tctcgcagac cgtcttttac cggcggccct catgaatggc
     4441 ccataggtca tgatggaggg ccgatggtca ggagcgaatg cgacgccgag cagggccgag
     4501 gggcccgttg cgagggaacg gcccgaggtt gaccgatagg tcaatcccga gggccactga
     4561 tataacgggg cgcgagggag atcacgatga acctgaaaca ttggctgggc gctgcggcgc
     4621 tgacgatcgg catggttgcg ggggcagggg ccgcctccgc ccagacggcg atcaagttca
     4681 cgctcgactg ggtgttccag gggccgacct cgccgtttct cgtcgcgctc gacaagggct
     4741 attacaaggc cgagggtctc gatgtgacga tggacccggg gcagggctcg gccggcgccg
     4801 tgcagcgcgt tgccaccggc gcctaccaga tcggctttgc cgatgtgaat tcgctgatcg
     4861 aatacaacgc caagaacccc ggcaaggaag tgctgtcggt gttcatggcc tatgattttc
     4921 cgcccttcgg cgtctacgcg ctgaagaaga gcggcatcag gacgctgaag gatctcgagg
     4981 gcaagaagct gggcgccccg gtgttcgatg cctcgttccg cctgttcccg gcctttgcca
     5041 agatcgtcgg catcgaccgg gccagggtgg aatcggtcaa cctgaccccg caattgcgcg
     5101 agcagagcct ggtgcagggc tcggtcgatt tcatttccgg gcattatttc tcgtcgatgc
     5161 ttgatctgaa ggcgcgcggc gtgaaggagg atgacgtcgt caagttcatg tattccgacc
     5221 atggcatgaa cgtttacggc aacggcatca tcattgcgcc cgaactcgcc cagaacgaaa
     5281 agctggtgca gggcttcatc cgcgccaccg tcaaagcctg gcgcgaggtt gcggccaatc
     5341 cgtcgatcgg cgtcgcggcg gtcaagaagc gtgatcccct ggccgatgag aagctggagg
     5401 ccgagcggct cgatatctcg ctgaaatcca acgtgctgac gccttacgtc aaagccaacg
     5461 gcatgggcga tgtcgatccc gagcgcttcg gcaaggcgct gaaggaagtg gctgacgcct
     5521 tcggcctgcc agccgcgccc gctgccgata aggtgttcac caaccgctat ctgccgccca
     5581 gggccgagcg gatggtggcg ccgtaagccg atgaccatcg ccggaacgga gccgccactg
     5641 gtcgagatgc gtgccgtcag cctcgcctac gggcgcggca aggatacgac gctggcgctg
     5701 gccgatgcca cgctctccat cgacaagggc gagttcatcg ccgtcgtcgg cccgtccggt
     5761 tgcggcaagt cgacgttgat gaagctggtt accggcctgc tgccgccgac cggcggcgag
     5821 atcctcgtgc acgggcagaa ggtgaccaga cccgtgcgcg gcgtcggcat ggcgttccag
     5881 aacccgacgc tgatgccgtg gcgcacgacg cgcgacaaca tcctgctgcc gctggaagtg
     5941 gtcgagccgc acaagcagcg cttccgcgcc aacaaggccg aatatgtcgc gcgggtcgag
     6001 gcgctgctga cctcggtggg gctcggcggc ttcggcgaca aatatccctg gcaattgtcg
     6061 ggcggcatgc agcagcgctc caacctctgc cgcgcgctgg tgcatgagcc ggaaatcctg
     6121 atgctggacg agccgttcgg cgcgctcgac gctttcacgc gggaagatct gtggggcgtg
     6181 atgcaggccc tttggatgga gcgcaaattc accgccgtcc tggtcacgca tgacttgagg
     6241 gaagcgacct atctcgctga tacaatttat gtaatgtcga aaagaccggg ccgcgtaatc
     6301 gtccgcaagc cgattgatat tccgcgcccg cgcacgctgg agacgacctt cgagccgcat
     6361 ttcgtcgaca ttgttcatga gctgcgcgac ctgatccggc aggagtatgc gtgatggcgc
     6421 aggcgtgccc tttgcccctc accccaccct ctccccgtta taaacgggga gagggagcgt
     6481 gcatgctgtt gccgctccgc tcttccctct ccccgtcaag aacggggaga gggggctgcc
     6541 gttgcctctc tgcttttccc tctcctcgca tgcggggaga gggtcagggt gaggggcgag
     6601 ggaaggaacc cgtcccatga atgacagcca acgccacctt ctgaaactgg ccatgccgtg
     6661 gctggtgatg gcggtcctgc tgctgatctg ggaactggcc tgcatcctgc tggctgttcc
     6721 ggagatcatc ctgccgcggc cgacgcgcat cttcgaggtg ttcgtccagc gcttcgacat
     6781 cctgctgcgc ttctgtctgg agacgttgtg gaccacggtg gcgggctttg ggctggcgat
     6841 cgtgttcggc ttgctgctgg ggctggcgat cggcgcctcg ccttttgtct attcggggct
     6901 gtatccgctg ctgatcgcgt tcaacgccat cccgaaggtg gcgattgtgc cgatcctgat
     6961 gatctggctc ggcgtcggcg caacgccggc gatcatcact gccttcgtca tctcgttctt
     7021 cccgattgtc gtcaatgtcg ccaccggcct tgccacgatc gaaccggaga tgcgcgatgt
     7081 gatgcgctcg ctcggggcga cgcaatacga aatcctgacc aaggtcggca ttccgcgcgc
     7141 catgccctat ctcttcgcct cgctcaaggt ggcgatcacg ctggccttca tcggctcggt
     7201 gatttccgaa acggtgggcg gcaaccgcgg catcggcttt ctgatgctgt cggccggcgc
     7261 acgcaacgat gcgccgacga cctttgccgg cctgtttgcc atcgccatca tgggggtggc
     7321 gatgtatgcg ctctgcgccc tgatcgaaaa gcgcatgacg cgctgggcgt tccgtggcga
     7381 aatggcggtg tgacgcgcca tgaccgccgc tcatcccgac gcccggttct gggaccggtt
     7441 cgcccggcgc tacgcagccg ccagcatccg ggatacggcg ggctatgagc gcacgctggc
     7501 gcgaacgtcc gaattgatcc ggggcatggc ctccgttctg gagatcggtt gcggcacggg
     7561 caccacggcc ttgcggatgg caggcggggt gcagcacctt ttggccaccg acatcgcgcc
     7621 gcagatgatc gcgattgccc gcgagaaggt ggcggcgcag gggtgctcca atgtcgcctt
     7681 cgaggtggcg gcgccggacg cgatcaccgc accggacggc ggctttgatg ccattctcgc
     7741 ctttaacgtc ctgcatctgc tgcgcgacga tgccgccacg ctggcccatc tgcacaccct
     7801 cctgaagccg ggcgggctgc tcatttccaa gacgccgtgc cttgtcgaac tcaacctgtt
     7861 catccgcctc gccattccgg tgatgcgccg gatcgggctc gccccgtatg tgcgggtgca
     7921 ggatgcggcc acgctggaga gcaggatcgc ggcggcgggc ttcacgatca tcgaacaggc
     7981 ccgccatggg tccaccggca aggacttccg caccttcctc gtcgcccggc ggcctgcgtg
     8041 ataggctcgc tgcatgggtc gttttttttc cgctgatttt tccgctcagg ccggcgttca
     8101 ggggctgatt gtcgcgctgg tcggctatgc ctcctccgcc gccatcgtca tcgccggcct
     8161 caaggccatg ggcgcgggca cggccgagat cgcctcgggt ctgctgctgc tcgggctggg
     8221 caagggcgtt gccgccatcg gactcagcct gtggacgcgg atgccgatct ccatcgcctg
     8281 gaccacgccg ggactggcgc tgctggcgac ctcgccgctg ccggcgggcg gatttggcac
     8341 ggctgtcgga gccttcatca tcaccggcct gctgttcgtg ctcgccggac tctggccgtt
     8401 gcttggccgg ctggtggccg ccattccgca tccgatcgcc agtgccatgc tggcgggcat
     8461 cctgctcaaa ctctgccttg cgccattcgt ggccttgcaa gcgctgccgc tgccggcgct
     8521 tgcgatcatc gcgacctggc tgctggtgca gcgcttcgcc cgcctctacg cggtgcccgc
     8581 tgcggtgctg gtgactcctt gcc
//
LOCUS       DAOMXG010000105         8485 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_4380_length_8485_cov_8.916963, whole genome shotgun sequence.
ACCESSION   DAOMXG010000105 DAOMXG010000000
VERSION     DAOMXG010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 8485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8485
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4380_length_8485_cov_8.916963"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..1285)
                     /gene="lpxB"
                     /locus_tag="PLQ11_08145"
     CDS             complement(116..1285)
                     /gene="lpxB"
                     /locus_tag="PLQ11_08145"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016920877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HRK24910.1"
                     /translation="MPVGGPLHVFLVAGEPSGDALGAKLIRALRQRRWNLQISGVGGE
                     AMAGEGLHSLFPMADIAVMGFVPVLKRLPTLIDRIRRTQRAVIEARPDVLVIIDSPDF
                     THRVARKVRAMRPDLPILNYVSPTVWAWRPGRAARMRAYIDHVLAVLPFEPEAHQRLG
                     GPACSYVGHPLIERFNELRPRPEDALQREFGRNVLILPGSRLSEVSRLMPVFGEAVAR
                     VAAEVPKAAFFLPAVAHVADAVRSALRDWPVKPEIVIGEQAKWMAFRRARAALAASGT
                     VTLELGLAQVPMVTAYKVSAIEKQIIRALVTVSTPILPSLILGEPVVPELLQEQATGA
                     ALAEALIPLVTGGEARSAQLAAFDRLDAAMQVTGGNPSAAAAEIVLYYGENGRRV"
     gene            complement(1294..2112)
                     /gene="lpxA"
                     /locus_tag="PLQ11_08150"
     CDS             complement(1294..2112)
                     /gene="lpxA"
                     /locus_tag="PLQ11_08150"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HRK24911.1"
                     /translation="MASNVHGSNIHVSAVIEDGAVIGADCSIGPFCHIGPHVTLGDGA
                     KLQSHVAIAGHTSIGPRATIYPFASVGHQPQDLKYHGEPVSLTIGADCIIREGVTINP
                     GTEGGGSKTVIGNNCAFLANAHVGHDCLIGNNVILVNNVMLAGHVTLGDFVICAGGSA
                     VIQFTRVGAHAFVGGLTGLENDLIPYGLAMGNRAHLAGLNMVGLRRRNFSREAIHDLR
                     RAYRLLFAEEGTLKERVEDVAAEFHAHPQVVEILDFIRKGGDRAICQPRREGEA"
     gene            complement(2114..2572)
                     /gene="fabZ"
                     /locus_tag="PLQ11_08155"
     CDS             complement(2114..2572)
                     /gene="fabZ"
                     /locus_tag="PLQ11_08155"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008547327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HRK24912.1"
                     /translation="MTDEGKTLETFDIMRLMELLPHRYPFLLVDKIINVRGDESGIGI
                     KNVTMNEPQFTGHFPIQPIMPGVLIIEGMAQTAGAMCVAARPSSAKLRAVYFMTIDKV
                     KFRKPVGPGDRLEYHMTKTAQRRNMWWFRGEAVVDGQKVAEAEVSAMLVD"
     gene            complement(2592..3632)
                     /gene="lpxD"
                     /locus_tag="PLQ11_08160"
     CDS             complement(2592..3632)
                     /gene="lpxD"
                     /locus_tag="PLQ11_08160"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018262668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HRK24913.1"
                     /translation="MTAPAFFPLPEPLSVARLIELTGATVAEGIDRSLSVRRVAPLEM
                     ATGGDLTFLENTAYADAAATTAASACFIAAKLKDRLAPGVVALVTAQPYRAYAQAAAV
                     LYPAAMRPMSLFGGAGVSPGASVHPTARLEEHVTVDPGAVIGPGAEIGARCIIGPGAV
                     IGPGVKLGRDASIGAGATVQHAFLGNRVIVHPGVRIGQDGFGFAMGPGGHVKVPQVGR
                     VIIQDDVEIGANTTIDRGTTRDTVIGEGTKIDNLVQIGHNVSIGRHCVIVSQVGISGS
                     STLEDYVVLAGQAGVAGHVRIGMGAQVGAKSGVMTDLAAGGRYLGAPAKPARQTMREV
                     AALARLAERGKE"
     gene            complement(3687..6251)
                     /gene="bamA"
                     /locus_tag="PLQ11_08165"
     CDS             complement(3687..6251)
                     /gene="bamA"
                     /locus_tag="PLQ11_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HRK24914.1"
                     /translation="MMKTIDGDSGVARRRARRAARTLAVGMLGLLASGVATLPALAQQ
                     IVVRGNTRTDAETIRSYLQLAPGESYSSARIDQAIKDLYATGMFSDVRINRSGGQIIV
                     QVAENTIINRVSFEGNSKLKAEVLQGEVQSRSRGPFSQALVDNDVQRILEIYRRSGRG
                     EAKVDARIVNSGNGRVDVAFVITEGGKTGVSAINFVGNNAYSASKLRNTMETTESGLF
                     GWLKTSDVYDPDRLAADVDRIRRFYLKNGYADFRVLSSSAEYSAEQKGYVITIAVEEG
                     EQYRVANVAVDSRVRDVDTGTLQRAVRTSQGDIYNAEAVEKSVEAIASETAKRGYAFT
                     QVRPRGDRDPSSRTVNIVYSVEEGPRVYVERINVRGNTRTRDYVVRREFDMGEGDAFN
                     KVYLDRAERRLKALGYFKNVKVSTEQGSSPDRVVINVDVEDQATGSFSISGGYSTSDG
                     FLAEAAVQETNFLGRGQYVKVSASNGQRSRGYEFSFTEPFFMDRRLAAGFDLFSKQTF
                     NSQYSRYETRTTGGTVRMSLPFTEEFSLGIRYSLYSQRLTIPNTNARPYNDCTFGLGE
                     GVTTVGGANYCLSNGEASVAVKEAAGRTVTSLAGLTLVYNTLDNPKDPTSGIYAELRP
                     EIAGLGGDSKFGRVTADARYYYPIADEIVGILRFQGGHISGFGKVNSTFGSSKLRMTD
                     HFFMGPNLVRGFAPGGIGPRDMNGDPTGNALGGTSYYGVSAEAQFSLPGVPRELGIKG
                     AVFADAGTLFGYRGARVFDVDGSGTLNGLGAGGTCPFTTLTVTSTQAECVNVRDKNML
                     RSSVGASLLWQSPLGPLRFDYAYALTKDKGYRDPVSGAYLGRDRLQAFRFSGGTRF"
     gene            complement(6432..7580)
                     /gene="rseP"
                     /locus_tag="PLQ11_08170"
     CDS             complement(6432..7580)
                     /gene="rseP"
                     /locus_tag="PLQ11_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HRK24915.1"
                     /translation="MFDILNAAGGHLWSLLGYLGPFLFVLALVIVVHELGHYLVGRWC
                     GVGVEAFALGFGTRLVGWTDRHGTEWKICAIPLGGYVKFKGDLNGASVPDEEALAAMD
                     PEERRTAFQFKPLWQRAAIVAAGPIANFILAFFIFLASLMSSGQYVVDPVIETVVAGT
                     AAEQAGIKPGDRIIAINGHKVATFDDVRRFVVQNPDDRALQMDIERSGIVTTLEARPR
                     VTEQKTSIGTLRSKTLGVTSSTRPEHQRVVHHGLIGSSVLAVEQIGGVISQSVNYLGG
                     LIAGREKADQLSGPIRIAQVSGKVAESGVSSLLVLVAILSISIGFINLMPVPLLDGGH
                     LLFYAVEGVFGRPLSRRVQEIGFRVGFALVLMLMLFVTINDIIQLRQG"
     gene            complement(7653..>8485)
                     /gene="dxr"
                     /locus_tag="PLQ11_08175"
     CDS             complement(7653..>8485)
                     /gene="dxr"
                     /locus_tag="PLQ11_08175"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003611199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HRK24916.1"
                     /translation="AALRRGATVALANKEAMVSAGAAVLEIARAGHATILPLDSEHNS
                     IFQALGGAPMDQVERIALTASGGPFRTWPLEAIAAATVEQALKHPNFAMGAKISVDSA
                     SMMNKGLELIEAHFLFGLPEDRLDVLVHPQQAIHGLVTFTDGSVNAGMAVPDMRVPMA
                     HCLAFPERSDSGAARLDLVAIGRLEFFAPDLDRFPALRLARQALRHGGAMATVLNAVN
                     EVAVAAFLEKSIGFLDIVKSVETVMNLPSMAALSAPRSIEEAEAIDRLARARAREVLS
                     "
BASE COUNT         1630 a         2867 c         2574 g         1414 t
ORIGIN      
        1 gcgaagtgga gcccggttcg cgcaaagaaa atgcgacaaa acaaaaagat agagcgcttt
       61 ttgtgaattg gaattcactg aaagcgctct agttttcgcc tcttcgctag tcttgtcaga
      121 cccgtcggcc gttctcgccg taatagagca caatttccgc cgctgccgcg ctgggattgc
      181 cgcccgtcac ctgcatggcc gcatccagcc ggtcgaaggc ggcaagctgg gcggagcggg
      241 cctcgcctcc agtgaccaga gggatcaggg cctcggccag agccgcaccg gtagcctgtt
      301 cctgcaacag ctccggcacg accggctcac cgaggatcag gctgggcagg atcggagtgg
      361 agacggtcac cagcgcgcgg atgatctgct tttcgatggc cgagaccttg taagcagtca
      421 ccatcggcac ctgcgccagc cccagttcca gtgtcaccgt gccggaggcg gcaagagccg
      481 cgcgggctcg gcgaaaggcc atccatttgg cctgctcgcc gatgacgatc tccggcttca
      541 ccggccagtc gcgcagcgcc gaccgcacgg catcggcgac atgggccacc gcaggcagga
      601 agaatgcggc cttgggaacc tccgccgcga cgcgcgccac cgcttccccg aacaccggca
      661 tcaggcggct gacttccgac aggcggctgc ccggcaaaat cagcacgttg cgcccgaatt
      721 cccgctgcag ggcgtcttcg ggccgcggac ggagttcgtt gaaccgttcg atcagcggat
      781 ggccgacata gctgcacgcc gggccgccca gccgctggtg cgcttccggt tcaaacggca
      841 ggaccgccag cacgtgatcg atataggcgc gcatcctggc cgcccggccc gggcgccagg
      901 cccagacggt cggtgaaaca tagttgagga tcggcagatc gggccgcatg gcccgcacct
      961 tgcgcgccac ccgatgggtg aagtccgggc tgtcgatgat gaccagcaca tcgggtctgg
     1021 cctcgatcac cgcccgttgt gtgcggcgga tgcggtcgat cagcgtcggc aaccgcttga
     1081 gcaccggcac aaagcccatg accgcgatat cggccatggg aaacaggctg tgcaatcctt
     1141 cccctgccat ggcctcgccg ccgacgccgc tgatctgcag gttccagcgg cgttggcgca
     1201 aggcacggat cagcttggcc ccgagcgcat caccggacgg ctcgccggcg acgagaaaga
     1261 catggagggg gccgccgacc ggcatgggcg cgctcaggcc tctccttcgc ggcgcggctg
     1321 acaaatcgcc cggtcgccgc ccttgcgaat gaaatcaagg atttcaacaa cctgcggatg
     1381 ggcatggaac tcagccgcga catcctcgac gcgctctttc agcgtgccct cttcggcaaa
     1441 cagcaggcgg tatgcgcgcc tgagatcgtg gatggcctca cgcgagaaat tgcgccgccg
     1501 cagcccgacc atgttgagcc cggccagatg ggcgcggttg cccatcgcca gaccataggg
     1561 gatcaggtcg ttctccagtc cggtcaaccc tccgacaaag gcatgggcgc cgacgcgcgt
     1621 aaactggatc accgccgaac cgccggcaca gatgacgaaa tcgccaagcg tcacatggcc
     1681 ggccagcatc acgttgttga ccagaatgac gttgttgccg atcaggcaat cgtgcccgac
     1741 atgggcattg gcgagaaagg cgcaattgtt gccgatcacc gtcttggagc cgccgccctc
     1801 ggtgccgggg ttgatggtga caccctcgcg gatgatgcag tcggcgccga tcgtcaggct
     1861 caccggctcg ccgtggtatt tcagatcctg cggctgatga ccgaccgagg caaagggata
     1921 gatggtcgcg cgcgggccga tcgatgtatg gccggcaatc gcgacatggc tttgcagctt
     1981 cgccccgtcg cccagggtca catgcgggcc gatatggcag aacgggccga tcgagcaatc
     2041 ggcaccgatc accgcgccgt cctcgatcac cgccgagaca tgaatgtttg agccatgaac
     2101 gttacttgcc atgtcaatcc accagcatcg cgctgacttc ggcctcggcc actttctggc
     2161 cgtcgaccac cgcctcgccg cggaaccacc acatgttgcg ccgctgggcc gttttggtca
     2221 tgtgatattc gagccggtcg cccggcccga caggctttct gaacttcacc ttgtcgatgg
     2281 tcatgaaata gaccgcccgc agcttggcgc tcgacgggcg cgccgcaacg cacatcgccc
     2341 cggccgtctg tgccatgccc tcgatgatca gcacgcctgg catgatcggc tggatcggaa
     2401 aatggccggt gaactgcggc tcgttcatcg tcacgttctt gatgccaatg ccgctctcgt
     2461 cgccgcgaac attgatgatc ttgtcgacca gcaggaacgg atagcgatgc ggcagcagct
     2521 ccatcagccg catgatgtcg aaggtctcga gcgtcttgcc ttcgtcggtc attccagccc
     2581 ccttttcccg gtcattcctt cccgcgttcc gccagccttg ccagcgccgc cacttcacgc
     2641 atcgtctgcc gggccggttt ggccggagcg ccaagataac gcccgcccgc ggccagatcg
     2701 gtcatcacac ccgacttggc accaacctgc gcgcccatgc cgatcctgac atgaccggca
     2761 acgccggcct gcccggccag cacgacgtaa tcctcaaggg tcgaagagcc ggaaatgccc
     2821 acctgcgaga cgatgacgca atgccgtcca atgctgacgt tatggccaat ctgcaccaga
     2881 ttgtcgatct tggtgccttc gccaatcacc gtatcgcggg tggtcccgcg gtcgatggtg
     2941 gtgttggcgc cgatctccac atcatcctgg atgatcaccc gtcccacctg cggcaccttc
     3001 acatgtccgc ctggacccat ggcaaagccg aacccgtcct gtccgatccg cacgcctgga
     3061 tgcacgatga cgcgattgcc gagaaaggca tgctgaaccg tcgcgccggc gccgatcgat
     3121 gcatcgcgcc cgagcttcac gccgggaccg atcaccgcgc ccgggccaat gatgcagcgt
     3181 gcgccgatct cggcgcccgg gccgatcacc gcgcccggat cgaccgtcac atgttcctca
     3241 agccgcgccg tcgggtgaac cgacgccccc ggcgagacgc ccgcgccgcc gaacagcgac
     3301 atcggtcgca tcgccgccgg atagagcaca gccgcagcct gcgcataggc ccgatagggc
     3361 tgcgcggtca ccaacgccac aacgccgggc gccagccgat ccttgagctt ggcagcgatg
     3421 aaacaggcgg aggccgctgt cgtcgccgca gcatcggcat aggcggtgtt ttctaggaag
     3481 gtcagatccc cgccggtcgc catctccagc ggagcgacac gccgcaccga caggctgcgg
     3541 tcgatcccct cggccaccgt ggcgccggtc aactcgatca gccttgcaac cgaaagcggc
     3601 tcaggcagcg ggaaaaaggc cggagccgtc atgagatatg attccgaagg cagacgggcg
     3661 ggaaggaccc cgcccggtca tcatgctcag aagcgggttc cgccggagaa ccggaaggcc
     3721 tgcagccggt cgcggccaag ataggccccc gagaccggat cgcggtagcc cttgtccttg
     3781 gtcagggcat aggcatagtc gaaccgcagc gggccaagcg gcgactgcca cagcaggctg
     3841 gcgccgaccg acgaacggag catgttcttg tcacgcacgt tgacgcattc ggcctgcgtg
     3901 gaggtcaccg tcagggtggt gaacgggcag gtcccgcccg cgccgaggcc attcagcgtg
     3961 ccgctaccat ccacgtcgaa caccctggcg ccgcgatagc cgaacaatgt gccggcatca
     4021 gcgaacaccg cgcccttgat gcccagttca cgcggcacgc cgggcagcga gaactgtgct
     4081 tccgccgaaa caccgtaata gctggttccg cccagcgcat tgccggtcgg atcgccgttc
     4141 atgtcgcgcg gaccgatgcc gcccggggca aagccgcgaa ccagattcgg ccccatgaag
     4201 aagtggtcgg tcatgcgcag cttggacgag ccgaaggtcg aattcacctt gccgaagccg
     4261 gagatgtggc cgccctggaa ccggaggata ccgacgatct cgtcggcaat cgggtagtag
     4321 tagcgtgcat cggccgtcac gcgcccgaac ttgctgtcgc cgccgaggcc ggcgatttcc
     4381 ggacgcaact cggcataaat gccgctggtc ggatccttgg ggttgtcgag cgtgttgtag
     4441 accagcgtca ggccggcgag cgacgtcacc gtgcggccag ccgcctcctt caccgcaacc
     4501 gacgcctcgc cgttggagag gcagtagttg gcaccgccaa ccgtcgtcac gccttcgccg
     4561 agaccgaacg tacagtcgtt gtagggtcgc gcgttggtgt tcgggatcgt cagccgctgc
     4621 gaatagagcg aatagcggat gcccagcgag aattcctcgg tgaacggcag cgacatgcgc
     4681 accgtgccgc cggtcgtgcg cgtctcatag cgcgaatact gcgaattgaa cgtctgcttc
     4741 gagaacagat cgaaacccgc cgccagacgg cgatccatga agaacggctc ggtgaacgag
     4801 aactcgtagc cacggctgcg ctggccgttc gaggcggaga ctttcacata ctggccgcgg
     4861 ccaaggaagt tggtctcctg cacggcagcc tcggcgagga aaccgtccga ggtcgaatac
     4921 ccgccggaaa tcgagaacga accggtggcc tgatcctcaa catcgacgtt gatgaccacg
     4981 cgatcgggcg acgagccctg ttcggtcgaa accttgacgt tcttgaaata gcccagcgcc
     5041 ttcaggcggc gctcggcacg atcaagatag accttgttga aggcatcgcc ctcgcccatg
     5101 tcgaactcgc ggcgcacgac ataatcgcgc gtgcgggtgt tgccgcgcac attgatgcgc
     5161 tcgacataaa cgcgcgggcc ctcttccacc gaatagacga tgttgacggt ccggctggac
     5221 ggatcacggt caccgcgcgg acgcacctgg gtgaaggcat agccgcgctt ggcggtctcc
     5281 gaggcaatcg cctcgaccga cttttccacc gcctcggcgt tgtagatatc cccctgcgag
     5341 gtgcgcacgg cccgctgcag cgtgccggta tccacgtcac ggacgcgcga atcgaccgcg
     5401 acattggcga cgcgatactg ctcgccttcc tcgacggcga tggtgatcac atagcccttc
     5461 tgctcggcgc tgtattcagc cgaagaggac aggacgcgga aatcggcata gccgttcttc
     5521 aggtagaagc ggcggatgcg gtcaacgtcg gcagccagac gatccggatc atacacgtcg
     5581 gacgtcttga gccagccgaa caggcccgac tcggtcgtct ccatcgtgtt gcgcagcttg
     5641 gaggcggaat aggcattgtt gccgacgaaa ttgatcgccg aaacaccggt cttgccgccc
     5701 tcggtgatca cgaaggcaac gtcaacgcgg ccattgccgg aattgacgat gcgcgcatcg
     5761 accttggcct cgccccggcc ggagcggcgg tagatctcga gaatgcgctg cacgtcattg
     5821 tcaaccagcg cctggctgaa tggtccgcgc gagcggctct gaacctcgcc ctggagcacc
     5881 tcggccttca gcttggaatt gccctcgaag ctgacgcggt tgatgatggt gttttccgcc
     5941 acctgcacga tgatctggcc gccggagcgg ttgatccgaa cgtccgagaa catcccggtg
     6001 gcataaagat ccttgatggc ctgatcgatc cgggcgctcg aataggattc gcccggcgcc
     6061 agctgcagat aggaccggat ggtctcggca tcggtgcggg tattgccacg caccacgatc
     6121 tgctgggcca atgccgggag ggtagctaca cccgaggcca gaagccccag catgccgacg
     6181 gccaaagtcc tggctgcgcg ccgcgcccgg cgcctggcca ctccgctgtc cccatcaatc
     6241 gtcttcatca tcgcttcgcc tatttccgcg gaaccgaacg catcatttct tggccgggcg
     6301 gcaccggcaa caaaccgttt acaaatgccg catcctgctt ctagcgggtc ggtctcccaa
     6361 tgcaaccaca aatccgtcgc aggttgattt tcggcgcctt cgtggccttc cggtcacgtc
     6421 ggctgtcggc atcatccctg ccgaagctgg ataatgtcgt tgatcgtcac gaacaacatc
     6481 agcatcagga ccagcgcaaa acccaccctg aagccgattt cctgcacccg gcggctcagc
     6541 ggacgcccga ataccccttc caccgcatag aacagcagat ggccaccatc gagcaaaggc
     6601 actggcatca ggttgatgaa gccgatcgag atcgacaaaa tcgcaaccag cactaacaag
     6661 gaggaaacac ccgattccgc caccttgccc gacacctggg cgattcggat cggaccggag
     6721 agctggtctg ccttttcccg gccggcgatc aggccgccga gatagttcac cgactggctg
     6781 atgacgccgc cgatctgctc caccgccagc accgacgagc cgatcagccc gtgatgaacc
     6841 acccgctggt gctccggcct ggtcgatgag gtcacaccca gcgtcttgga gcgcaacgtg
     6901 ccgatcgagg tcttctgctc ggtcacgcgc ggccgcgctt ccagcgtggt gacgattccg
     6961 ctgcgctcga tatccatctg cagcgcccgg tcatccggat tctgcaccac gaaacggcgc
     7021 acgtcatcga aggtcgccac cttgtggccg ttgatcgcga tgatgcggtc gcccggcttg
     7081 atccctgcct gctccgctgc cgtgccggcc acgaccgtct cgatcaccgg atcgacgaca
     7141 tactggccgg acgacatcag gctggccagg aatatgaaga aagccaggat gaaattggcg
     7201 atcggcccgg ccgcaacaat cgcggcgcgc tgccacagcg gcttgaactg aaacgccgtg
     7261 cggcgctctt ccgggtccat cgccgccagc gcttcctcat ccggcaccga ggcaccgttg
     7321 agatcgccct tgaacttgac atagccgccg agcggaatcg cgcagatctt ccattcggtt
     7381 ccgtgccggt cggtccagcc gaccagccgg gtgccgaagc caagcgcgaa agcctcgacc
     7441 cctaccccgc accagcgccc gaccagataa tggccaagct catgcacgac aatcaccagc
     7501 gccagaacga acagaaacgg acccagatag cccagcaggc tccagaggtg gccgccggcc
     7561 gcattcagga tatcaaacat gcaatcctcc aggcgccatg accgcatcaa actccggcac
     7621 cgagcgggcc aaaatgtgcc atgcgcagcc cctcaggaca acacctcgcg ggctctagcc
     7681 ctcgccagac ggtcaatcgc ctcggcctct tcgatcgacc tgggagcaga caaagcggcc
     7741 attgacggca gattcatgac cgtttccaca cttttaacta tatcgagaaa tccaatcgat
     7801 ttttctaaaa atgccgccac cgcgacctcg ttgaccgcgt tgagaaccgt cgccatggcc
     7861 ccgccatgcc gcagcgcctg ccgggccagc cgcaatgccg gaaaccggtc aaggtccggc
     7921 gcgaagaatt ccagccggcc gatggcgacc agatcgagcc gcgccgcgcc cgagtcgctc
     7981 cgctccggaa aggccaggca atgggccatc ggcacccgca tgtcgggaac ggccatgccg
     8041 gcattgacgc tgccatcggt gaaggtgaca agcccgtgga tcgcctgctg cggatgcacc
     8101 agaacgtcga gccgatcctc cggcagaccg aacaggaaat gcgcctcgat cagttccagc
     8161 cccttgttca tcatcgaggc ggaatcgacg ctgatcttgg cgcccatggc gaagttcgga
     8221 tgcttgagcg cctgctcgac cgtggcagcg gcaatcgctt ccagcggcca ggtgcggaac
     8281 ggtccgcccg aggcggtcag ggcaatccgc tcgacctgat ccatcggcgc gccacccagt
     8341 gcctggaaga tcgaattgtg ctcggaatca agcggcagga tggttgcatg cccggcgcgc
     8401 gcgatctcca gcaccgcagc gccggccgag accatcgcct ccttgttggc gagcgccacc
     8461 gtcgcaccgc gccgcagcgc cgcac
//
LOCUS       DAOMXG010000106         8295 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_4500_length_8295_cov_10.427670, whole genome shotgun sequence.
ACCESSION   DAOMXG010000106 DAOMXG010000000
VERSION     DAOMXG010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 8295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8295
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4500_length_8295_cov_10.427670"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="PLQ11_08180"
     CDS             <1..693
                     /locus_tag="PLQ11_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRK24917.1"
                     /translation="IGLALACALAEAGAHVTLCARSGDELDAAVAALRGRGDTAEALP
                     LDVTDQVAVEAALSARAPFDILVNNAGMNRPAPFVDVDMADFDRIMGLNVRAAFALSQ
                     RVARGMIEAGRPGAIIYISSQMGHVGGIDRTVYAASKHAIEGLTKAMAIDLGPKGIRV
                     NSIGPTFIRTDLTASTFANPERLAWIRSKIKLDRIGEVEDIMGAVVFLASDAAALITG
                     TSLLIDGGWTAG"
     gene            712..1698
                     /locus_tag="PLQ11_08185"
     CDS             712..1698
                     /locus_tag="PLQ11_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009464719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HRK24918.1"
                     /translation="MPIKGFDPRWKDFPDYIIGITKEIWEDRGIAALHQYYAPDIVVR
                     SPASIVRGNQTVIGATMATLAEFPDRQLLAEDVIWSEHASGLLSSHRIVSTATHARDG
                     VYGKATGKTLTYRIIADCAAKANQIFDEWLIRDQGAIVRQMGWEPKAYAADLIQREGG
                     PEACVKPFAPDRDMAGGYTGKGNDHAVGQLYADILTRIMGADMAAIRTEYDRAAQLEL
                     PGGVTGHGWEAADRFWMGLRAALPDATFTIHHVIGREDAMMPPRAALRWSLTGRHSGW
                     GAFGTPTGADIHVMGISHAEFGPFGGENGSLRREWVLFDETAIWKQILLKAG"
     gene            1771..2739
                     /locus_tag="PLQ11_08190"
     CDS             1771..2739
                     /locus_tag="PLQ11_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRK24919.1"
                     /translation="MTPQEMEARIVRYGDLKPCKTAFIDAHTPGSNQKENFTIIGGGV
                     SESPDQHVHIRETPGFNIGAAGQPPGCRNSLHSHRTAEVFFVLKGRWRFFWGRWGTAG
                     EFIAEEGDIFNIPTGIFRAFENVGTDYGMIMAILGGDDAGGGVIWAPQVITDAKDYGL
                     VLGKNGRLYDTKKGQKLPDGVPPMPLLTDEELKAYPEPTTADVIPNCVARYWDLMALS
                     DRQPAKVIGEHGKLKDRPGFEVEFLSRNSISATLYSLNRHEVLMPMRGHWRLGWSGGS
                     TVLNPGDTVAIPPGLEHSLAPSMTGEASLYRVINTDDPAGATGRLL"
     gene            2750..3871
                     /locus_tag="PLQ11_08195"
     CDS             2750..3871
                     /locus_tag="PLQ11_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008034761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HRK24920.1"
                     /translation="MSLMATHVGSLPRSQKVVDFLFARERNEPYDVAAFDAAMAEAVM
                     EVVRRQKEAGIDIVSDGETSKISYATYVKDRYTGFDGDSPRNAPADLKLFPSFLERLA
                     KAGGTPQYARPQCVGPVRSKGQGELQKDIAALKAAMAAHGVQRGFMNAASPGVISLFL
                     QNAHYATREAYLAALADAMRDEYRTIIEAGLDLQLDCPDLALSRHMLFTHLSDDDFLK
                     VAGLHVEALEHALTGLPQDRIRLHICWGNYEGPHCCDIDMGKVFPLLMRVPARYILFE
                     TANPRHGHEWTVFRDRAREIPADKILVPGAVDTTTNFIEHPELVRQRLERFTAIVGRD
                     RVIAGSDCGFGTFAGFGAVDPEIAWAKLATLKEGARRVA"
     gene            3868..4341
                     /locus_tag="PLQ11_08200"
     CDS             3868..4341
                     /locus_tag="PLQ11_08200"
                     /inference="COORDINATES: protein motif:HMM:NF013166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase domain-containing protein"
                     /protein_id="HRK24921.1"
                     /translation="MSPLVLVPGMMCDARLWGDLPGALGRRMVIHAPCTEADTVAGLA
                     AAILRDAPPRFALAGLSMGGIVAMEMLRQAPERVERLALLDTNPFAERPEMRARRQGQ
                     IDRALAGDLAAVIRDEMKPNYLALGTRQSAGAGPLLRDGAGARAGCLCASVAGSA"
     gene            4316..4558
                     /locus_tag="PLQ11_08205"
     CDS             4316..4558
                     /locus_tag="PLQ11_08205"
                     /inference="COORDINATES: protein motif:HMM:NF019982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRK24922.1"
                     /translation="MRQSRALRDRPDQSATLRAFKGPALVLMGEHDRLCPLERHEAMH
                     RMMPQSCLAIIADAGHLPTLEQPQASVAEIEGWLEA"
     gene            complement(4923..5007)
                     /locus_tag="PLQ11_08210"
     tRNA            complement(4923..5007)
                     /locus_tag="PLQ11_08210"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4971..4973),aa:Leu,seq:caa)
     gene            5139..5720
                     /locus_tag="PLQ11_08215"
     CDS             5139..5720
                     /locus_tag="PLQ11_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020091426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HRK24923.1"
                     /translation="MLKRLYDWTIRLSESPRAPHALGVVSFAESSFFPIPPDVMLLPM
                     AMAKPHKAWFYATICTVTSVLGGIVGYAIGALLYDSLGQWLIKVYGLGDKLDSFRALY
                     QQWGHWIILIKGFTPIPYKLVTIASGLAAYDFFWFVILSIITRGGRFFFIAGILNRFG
                     APLKQFIEDNLTLVAIGLLVAIVGGFYVVKFLI"
     gene            5733..6260
                     /locus_tag="PLQ11_08220"
     CDS             5733..6260
                     /locus_tag="PLQ11_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007558875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HRK24924.1"
                     /translation="MGAMPLRITPALLALGLAAASLIGAWVFQYGLGYAPCKLCLLQR
                     WPYYIGLVLGAGGLITYGLGHDRTAMAFWALFALTFLVGAGLGIYHAGVEWKYWAGPT
                     DCGGAFNAMPKQVTDLRAALATTKVVRCDEAPLRILGLSFAGWNVVVSLVVAGLAGRA
                     AMGGTGRYGSSSLSQ"
     gene            complement(6233..6802)
                     /locus_tag="PLQ11_08225"
     CDS             complement(6233..6802)
                     /locus_tag="PLQ11_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HRK24925.1"
                     /translation="MTVHVQPAISPEACPALVLNADYRPLSYYPLSLWSWQDALKAVF
                     LDRVNIVSEYSRVVHSPSMSIRLPSVVSLKSYVQPSRSPAFTRFNVFLRDRFSCQYCG
                     SRDDLTFDHVIPRSKGGLTTWENVVAACSPCNLRKGDKLPDEALMRPRNRPFAPSLSQ
                     LHASGRHFPPNYLHESWLDYLYWDSELEP"
     gene            complement(6900..7781)
                     /locus_tag="PLQ11_08230"
     CDS             complement(6900..7781)
                     /locus_tag="PLQ11_08230"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HRK24926.1"
                     /translation="MALEIRKIGIVGAGQMGSGIAHVAALNGFDVLVHDVAQDRINAG
                     LATINGNLSRQVQKGAIGDADKKAALARIDGAADLAGLTDCDLVIEAASENETIKRKI
                     FQDLSRLLKPEALLASNTSSISITRLASTTDRPEKFIGIHFMNPVPVMQLVELIRGIA
                     TDDATFNAAKTFIAKLGKTVTVSEDFPAFIVNRILLPMINEAIYTLYEGVGTVEAIDT
                     AMRLGANHPMGPLQLADFIGLDTCLAIMQVLYEGLADSKYRPCPLLVKYVEAGWLGRK
                     AKRGFYDYRGDKPVPTR"
     gene            complement(7844..>8295)
                     /locus_tag="PLQ11_08235"
     CDS             complement(7844..>8295)
                     /locus_tag="PLQ11_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRK24927.1"
                     /translation="LAGGGAVAGATFVSEELSKSDRPELASAKIIVSGGRALASSENF
                     GKFIEPVADKLGAAVGASRAAVDAGYAPNDWQVGQTGKVVAPDLYVAVGISGAIQHLA
                     GMKDSKVIVAINKDADAPIFQVADYGLVGDLFQVMPDLAAALDRAKG"
BASE COUNT         1504 a         2564 c         2672 g         1555 t
ORIGIN      
        1 atcgggctgg cgctggcatg cgcgttggcc gaggccgggg cgcatgtgac gctctgcgcg
       61 cgttctgggg acgaactgga tgctgctgtc gcggcgctgc ggggccgtgg cgacaccgca
      121 gaggcgctgc cgctcgatgt cacggatcag gttgcggttg aggccgcgct atccgcgcgg
      181 gcgcccttcg acatcctcgt caacaatgcc ggcatgaacc ggcccgcgcc gttcgttgat
      241 gtcgatatgg ccgatttcga ccggatcatg ggcctcaatg tccgcgctgc cttcgcgctg
      301 tcgcagcggg ttgcccgcgg gatgatcgaa gcaggcaggc cgggggcgat catctacatc
      361 tcctcgcaga tggggcatgt cggcggcatc gaccgcacgg tctatgccgc gtcgaagcat
      421 gcgatcgagg gtttgaccaa ggcgatggcg atcgacctgg ggcccaaagg catccgcgtg
      481 aactccatcg gcccgacctt catccgcacc gatctgaccg cctcgacctt cgccaacccg
      541 gagcggctcg cctggatccg atcgaagatc aagctcgacc ggatcggcga ggtcgaggac
      601 atcatggggg cggtggtgtt ccttgccagc gatgccgccg ccctcatcac cggaacctca
      661 ttgctgatcg atggcggctg gaccgccggc tgacttttgg agaccaggac catgcccatc
      721 aaggggttcg acccgcgctg gaaggacttc cccgactaca tcatcgggat cacgaaggag
      781 atctgggagg atcgcggcat cgcggccctg caccagtact acgcgccgga catcgtggtc
      841 cgttcgcccg cctccatcgt gcgcggcaac caaactgtca tcggcgcgac gatggcgacg
      901 ctcgccgaat tcccggaccg gcagttgctg gccgaggacg tgatctggag cgagcatgcc
      961 agcgggctcc tgtcctcgca ccgcatcgtc tcgacggcga cgcatgcccg cgacggcgtc
     1021 tatggcaagg cgaccggcaa gaccctgacc tatcgcatca tcgccgattg cgcggcgaag
     1081 gcgaaccaga tcttcgacga gtggctgatc cgcgatcagg gcgccatcgt gcgccagatg
     1141 ggctgggagc cgaaggccta tgccgctgac ctgatccagc gcgaaggcgg gccagaggct
     1201 tgcgtcaagc cgttcgcgcc cgaccgggac atggcgggcg gctataccgg caaaggcaat
     1261 gatcacgcgg tcggccagct ttatgccgac atcctcaccc ggatcatggg cgccgacatg
     1321 gccgcgatcc gcaccgaata tgaccgcgcg gcgcaactgg aactgccagg cggcgtcacc
     1381 ggtcatggct gggaggcggc cgaccggttc tggatgggcc tgcgtgccgc cttgcccgat
     1441 gccaccttca ccatccacca tgtgatcggt cgcgaggacg ccatgatgcc gccgcgcgcc
     1501 gcgctgcgct ggtcgctcac tggaagacat tccggctggg gtgccttcgg tacgccgacc
     1561 ggtgcggaca ttcatgtcat gggcatcagc catgccgagt tcgggccctt cggcggcgag
     1621 aacggctcgc tgcggcgcga atgggtgctg ttcgacgaaa ccgccatctg gaagcagatc
     1681 ctgctgaaag caggctgaac cgatctgcct gtgcgcgcgt ccgtgcgtgg ccgtttgcat
     1741 acgaatgcag tctgaggaaa ggacaagaga atgaccccgc aagagatgga agcgcgcatc
     1801 gttcgctatg gcgatctgaa gccatgcaag accgccttca tcgatgccca tacgccgggc
     1861 tcgaaccaga aggagaactt caccatcatc gggggggggg tgtcggagag ccccgatcag
     1921 catgtgcata tccgcgagac gccgggcttc aacatcggcg ccgccggtca gcctcccggc
     1981 tgccggaact cgctgcattc gcaccgcacg gcggaggttt tcttcgtgct gaagggccgc
     2041 tggcgcttct tctgggggcg ctggggcacg gcgggcgagt tcatcgccga ggagggtgac
     2101 atcttcaaca tcccgaccgg catcttccgg gccttcgaga atgtcggcac cgattacggg
     2161 atgatcatgg cgatccttgg aggcgatgac gccggcggcg gcgtcatctg ggcgccgcag
     2221 gtgatcaccg atgccaagga ttacggcctc gtgctcggca agaacggcag gctctatgac
     2281 accaagaagg gccagaagct tcccgatggc gtgccgccca tgccgctgct gacagacgaa
     2341 gagctgaagg cctatcccga gcccacgacg gccgatgtga tccccaattg cgtcgcccgc
     2401 tactgggacc tgatggcgct ctccgaccgg cagccggcca aggtgatcgg cgagcacggc
     2461 aagctcaagg accggcccgg tttcgaggtc gagttcctgt cgcgcaattc gatctccgcg
     2521 acgctctaca gcctgaaccg gcatgaagtc ctgatgccga tgcgcggaca ctggcgcctt
     2581 ggctggagcg ggggcagcac ggtgctgaat ccgggcgata cggtggccat cccgcccggc
     2641 ctggagcatt cgctcgcgcc atcgatgacc ggcgaagcct cgctctatcg cgtgatcaac
     2701 acggatgatc cggccggcgc gaccgggcgg ctgctctgag gaggctgtca tgtcgctgat
     2761 ggccacccat gttggctcgc tgccccgctc gcagaaggtt gtggattttc tcttcgcgcg
     2821 cgagcgcaac gaaccctatg acgtcgccgc cttcgatgcg gcgatggcgg aggccgtgat
     2881 ggaggtcgtc cgccggcaga aggaggcggg gatcgacatc gtctcggacg gcgagacctc
     2941 gaaaatctcc tacgcgacct acgtgaagga tcgctacacc ggcttcgatg gcgacagtcc
     3001 gcgcaatgcg ccggccgatc tgaagctgtt tccgagcttc cttgaacgtc tcgccaaggc
     3061 cggcggaaca ccgcaatatg cgcgtccgca atgtgttggc cccgtccgct cgaaggggca
     3121 gggtgagttg cagaaagata tcgcggcgct gaaggccgcg atggctgccc atggcgtgca
     3181 gcgtggtttc atgaatgctg cctcgccagg cgtcatctcc ctgttcctgc agaatgcgca
     3241 ttatgcgacc agagaggcgt atctggccgc actggccgat gcgatgcgcg acgaataccg
     3301 gaccatcatc gaggctgggc tcgatctcca gctcgactgt ccggatctcg ccctgtcccg
     3361 gcacatgctc ttcacccatc tctccgacga tgacttcctg aaggtggccg gtctgcatgt
     3421 cgaggctctc gaacacgcac tgacggggct gccgcaggac cgtattcgcc tgcatatctg
     3481 ctggggcaac tacgaagggc cgcattgctg cgacatcgac atggggaaag tgttcccgct
     3541 gctgatgcgc gtgcccgcac gctacatcct cttcgagacg gccaatccgc ggcacggtca
     3601 tgaatggacg gtgttcaggg accgggcgcg cgagatcccg gctgacaaga ttctcgtgcc
     3661 cggcgcggtc gatacgacga ccaatttcat cgaacaccct gaacttgtgc gccagcggct
     3721 ggaacgcttc accgccatcg tcggccgcga ccgggtgatc gccggttccg attgcggttt
     3781 tggcaccttt gccggcttcg gcgcggtcga tcccgagatc gcctgggcga aactcgccac
     3841 gctgaaggag ggcgcaaggc gcgtcgcatg agcccgctcg tgctggttcc aggcatgatg
     3901 tgtgatgcca ggctgtgggg cgatctgccc ggagcgctgg ggcggcggat ggtgatccat
     3961 gcgccgtgta ccgaagccga cacggtcgcc gggctggccg ccgcgatcct gcgcgatgca
     4021 ccgccgcgct ttgcgctggc gggcttgagc atgggcggca tcgtcgcgat ggagatgctg
     4081 cggcaggcac cggaacgggt ggagcgcctc gcgcttctcg acaccaaccc ctttgccgaa
     4141 cggcccgaga tgcgggcgcg tcggcagggg cagatcgacc gggcgctggc cggggatctc
     4201 gccgccgtta tccgcgacga gatgaagccc aactatctgg ccctcgggac ccggcaatca
     4261 gccggtgctg gacctctgct acgcgatggc gctggcgctc gggccggatg tctttgtgcg
     4321 tcagtcgcgg gctctgcgtg atcgcccgga tcagagtgcg acgctgcgcg ctttcaaggg
     4381 gccggcgctg gtgctgatgg gagaacatga ccggctgtgc cctctggaac gtcacgaggc
     4441 gatgcaccgg atgatgccgc aatcgtgctt ggcgatcatc gctgatgccg ggcatctgcc
     4501 gacactcgaa cagccacagg cgagcgtcgc cgagatcgaa ggttggctcg aagcatgacg
     4561 acgtcggtaa taccgaccac cagcgctttg cctacagcaa ttgcctgccg ctgcctgagt
     4621 ttgcacaccg cacgcgcgga acgggatgct gtcacccgtc gagttcgtct ccaccttcaa
     4681 cccgcgtcgg gctctggcgc tgcgcaatgt gaaaagctcc gcgccatcgc ccgccgcatc
     4741 caccacccat cagggcaaac ccaaccccgc aaacgaactc agaactggat aatacttggg
     4801 ggcaacgtca gctgaatttt tagctatgtg agagcgcctt tctgaaggtg cctccgccaa
     4861 gcgtcggaag tgtcgaggca atttcttcga attaagcaat tgatatcatt ttgaaaaacg
     4921 gttggtgccc ctagccggaa tcgaaccagc actccttgcg gaaaccgatt ttgagtcggt
     4981 cgcgtctacc agttccgcca taggggcaac gcaggcggac tatagcgagc ggggccttcg
     5041 ggtcaacgcg gcagaaggga aaatccgtgt tttgtcgaag gcctccgttt tagaaatcgg
     5101 gctttacctg aaccgatctg ccagctatcc cccgactcat gctgaagcgc ttatacgact
     5161 ggaccatcag actgtcggaa tcgccgcgcg caccccatgc ccttggtgtg gtgtcgttcg
     5221 ccgaaagttc gttcttcccg atcccgccgg atgtcatgct gctgccgatg gcgatggcca
     5281 agccgcacaa ggcctggttc tatgcaacga tctgtaccgt gacatcggtg ctgggcggca
     5341 tcgtcggcta tgccatcggc gcactgcttt acgacagcct cggccaatgg ctgatcaagg
     5401 tctatgggct gggcgacaag cttgattcct tccgcgcgct ctatcagcaa tggggccatt
     5461 ggatcattct catcaagggt ttcacgccga tcccctacaa gctggtgacg atcgcctccg
     5521 gactggcggc ctatgatttt ttctggttcg tcatcctgtc gatcatcact cgcggtggac
     5581 gcttcttctt tatcgccggg attctcaacc ggttcggagc gccactgaaa cagttcatcg
     5641 aagataatct gacccttgtg gcgatcggct tgctggtggc gattgtcggc ggcttttacg
     5701 tggtcaagtt cctcatctga ctggagaggc acatgggcgc tatgcccttg cgaatcacac
     5761 cggcgctgct tgctctgggg ctggcggccg cctcgctcat cggggcatgg gtcttccaat
     5821 atggattggg ctatgcgccg tgcaaattgt gcctgttgca gcgttggccc tattacatcg
     5881 ggcttgtcct gggtgcgggc gggctgatca cctatggcct ggggcatgac cggacggcga
     5941 tggcgttttg ggccctgttt gccctgacct tcctggtcgg cgccggactt ggcatctatc
     6001 acgccggcgt cgagtggaaa tactgggcgg ggccgaccga ttgcgggggc gccttcaacg
     6061 caatgcccaa gcaggtgacg gacctgcggg ccgcgcttgc aacaaccaag gtggttcgct
     6121 gcgatgaagc accgttgcgc attcttggcc tgtcatttgc cggctggaat gtcgtggtgt
     6181 cgctggtggt ggccggactt gcgggccggg cggcgatggg cgggacaggt cgctacggtt
     6241 ccagttcgct gtcccaatag agataatcga gccagctctc atgcagataa ttgggcggaa
     6301 agtgtcggcc gctggcgtgc agctggctga ggctgggagc aaagggccgg ttgcgcggtc
     6361 gcatcagagc ttcatcgggc agtttgtcgc ccttcctgag gttgcacggc gaacaggccg
     6421 cgacgacatt ctcccaggtg gtcagcccgc cttttgatcg cgggatcaca tgatcgaagg
     6481 tcagatcgtc ccgactgccg caatattggc aggaaaagcg atcgcgcagg aagacgttga
     6541 accgggtaaa ggccgggctg cgcgatggct ggacatagga cttcagcgat acgaccgatg
     6601 gaagccggat cgacatcgat gggctgtgaa cgacgcggct gtattcggag acgatgttga
     6661 cccgatcgag aaacaccgcc ttgagtgcat cctgccagga ccagagcgac aaaggataat
     6721 agctcagcgg ccggtaatcc gcattgagca ccagcgccgg acaggcttcc ggcgagattg
     6781 cgggctggac gtgaaccgtc acaccgctac tcccacgatc cgaatcgcac cattcccaaa
     6841 aggcgccgct atgtaagcag gccaagaacg gcttgtgaag gcggatcagt cgcggagtat
     6901 cagcgcgtcg gcaccggctt gtcgccgcga tagtcgtaga agccgcgctt ggccttgcgg
     6961 cccagccagc cggcctcgac gtatttcacc agcaacgggc agggacgata tttggaatcc
     7021 gccagccctt catagagcac ctgcatgatc gccagacagg tatcaaggcc gatgaaatcg
     7081 gccagttgca acggccccat cgggtgattg gccccgagcc gcatggccgt atcgatggct
     7141 tccaccgtgc cgacaccctc atagagcgta tagatcgcct cgttgatcat cggcagcaga
     7201 atgcggttga cgatgaaggc ggggaaatcc tccgagacgg tcaccgtctt gccgagtttg
     7261 gcgatgaagg tctttgccgc gttgaaggtc gcatcgtcgg tggcgatgcc gcggatcaac
     7321 tcgaccagct gcatcaccgg caccggattc atgaaatgga tcccgatgaa cttttccggc
     7381 ctgtcggtgg tcgaggccaa tcgggtgatc gagatcgacg aggtgttgga ggccagcagc
     7441 gcctcgggtt tgagcagacg cgacaaatcc tggaagatct tgcgcttgat cgtctcgttt
     7501 tcgctcgccg cttcgataac cagatcgcag tccgtcaggc cggcaaggtc ggcggcgcca
     7561 tcaatgcggg caagcgcagc cttcttgtcg gcatcgccga tggccccctt ctgaacctga
     7621 cgcgaaagat tgccgttaat ggtcgcgagt ccggcgttga tccggtcctg cgccacatca
     7681 tgaaccagca catcaaagcc gttgagcgcg gcgacatgag caatgccgct gcccatctgg
     7741 ccagcgccga caatgccgat cttgcgaatt tccaacgcca tggcagtcca ttccgtgcgt
     7801 gcaggttcag aaaaaaaacg gggcgcaagg ccccgttctg tgatcagcct tttgctctgt
     7861 cgagagcggc ggccaggtcg ggcatgacct ggaaaagatc cccgacgagg ccataatcgg
     7921 caacctggaa gatcggcgca tcggcatcct tgttgatggc gacgatgacc ttggaatcct
     7981 tcatgccggc aagatgctgg atggcgccgg aaatgccgac cgcgacatag aggtcaggcg
     8041 cgacgacctt gccggtctgg ccgacctgcc agtcgttggg ggcatagccg gcatcgaccg
     8101 ctgcgcgcga ggcacccacc gccgcgccga gcttgtcggc taccggctcg atgaacttgc
     8161 cgaagttctc gcttgaggcg agcgcgcgac cgccggagac gatgatcttg gccgaggcca
     8221 gttccggccg gtcggacttg gacaattcct cgctgacaaa agtcgccccg gccacggcgc
     8281 cgccacccgc caacg
//
LOCUS       DAOMXG010000107         8267 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_4527_length_8267_cov_9.960058, whole genome shotgun sequence.
ACCESSION   DAOMXG010000107 DAOMXG010000000
VERSION     DAOMXG010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 8267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8267
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4527_length_8267_cov_9.960058"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="PLQ11_08240"
     CDS             complement(<1..425)
                     /locus_tag="PLQ11_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015952655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HRK24928.1"
                     /translation="MNHVDLRLYAILDPERTGGRPLDRLADAAAAGGATLLQLRDKNA
                     GTRSFVARARQVKAALAGRIPLIINDRIDVALAAEADGVHLGRDDMHPADARRLLGPE
                     AIIGVTLKNNADLAVLDPALVDYGCIGGVFATASKNNPDP"
     gene            complement(422..1150)
                     /locus_tag="PLQ11_08245"
     CDS             complement(422..1150)
                     /locus_tag="PLQ11_08245"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: protein motif:HMM:NF014198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HRK24929.1"
                     /translation="MSNEPIILSMAEVLTRLRTGRVRVHALTSPVAAERTANTLLALG
                     ARPSLTANREEIAGFVGGCDALLINLGMLDAERRDTAPLAAALARQGGKPFVLDPVFV
                     DVSGPRRSLAAELVAAGPTVVKLNRAEAASLLAIPATAVTVVTGAIDTLSQAGRTLRL
                     ANGHPLAGQVTATGCALGAVIAACLAVEPDAMTATAAALALYGIAAERAAEGATGPGS
                     FAVRLIDALATLDPATLAERVRFA"
     regulatory      complement(1142..1248)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(1503..2507)
                     /locus_tag="PLQ11_08250"
     CDS             complement(1503..2507)
                     /locus_tag="PLQ11_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRK24930.1"
                     /translation="MTTAPSSLPPPQQPAARPLFGIGLKIASALAFTAMSGLVRYVAT
                     APDFTRVPLGELVFFRSLFALLPVLLWLAYARPLSAAFVTTNLRGHIRRGLIGALGMF
                     LGFAGLQYLPLPDATALSYTTPLLTVVLAWLLLKEVIRIYRWTAVIVGLVGVLIMLTP
                     HLSPTHLAGSSGSWKGVALALAASVCAAFATIEVRKLTATEHTGAIVVYFSLTTTVLS
                     LVSFGLGHLWPSHAWVWPPSGDFAMLAMIGFLGGIGQILLTHCYRYADASIIAPFDYT
                     SMIWALLIGWLVFSEIPDRLVLIGATVVIASGIFVILRERALGIERRSQREASPTRPL
                     "
     gene            complement(2627..3427)
                     /locus_tag="PLQ11_08255"
     CDS             complement(2627..3427)
                     /locus_tag="PLQ11_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24931.1"
                     /translation="MSSSSPEMPQFRDFAAIEAAVRQTERGRWFLAEFASRQRRDDAA
                     AILAALDRVDRQVKALSDRDHTLPFRKALTEIAAAIAATQGEIGCHSPGVGCPGRLIT
                     ASGELDAVVRAAEAATASILTAAEKMQEQAWTLRERGGAPLDCDALEASATEIYLACA
                     SQDIAAQRIRKVTETLRFVETRIATLLDVLGIERCAKAYPQAAEDSPVFDQPRPLWPD
                     TPGVQSAVDAILADPDIRPAATEGAPPRRSGLSVLDMLTPDERLRLFV"
     gene            3523..4689
                     /locus_tag="PLQ11_08260"
     CDS             3523..4689
                     /locus_tag="PLQ11_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019903013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family lipid ABC transporter permease
                     subunit"
                     /protein_id="HRK24932.1"
                     /translation="MPAPLTPEAQRLTLKPDALVSREGDFVRVTLSGDWSAANAHECE
                     DEADAVLTAVKGASRVVIDASGIARLDTLGTWVLSRVGHDVIESGSRLAVTGASPAHR
                     LLFDEVQYRRQQEPPPPRGIVGFDILSDIGEAMVDAFRDLVNGIAFLGAIVAAWGRIL
                     TGRSRFRLAALVGQLERVGLRSVPIIVLISLLVGGIIAQQSVFQLQKFGTVHFVADGL
                     GVLVLRELAVLITAIMIAGRTGSSFTAELGSMKMREEIDALRTMGLNPIEVLVVPRIL
                     ALLIGLPLLTFISSMAMLFGGGLVAMIYGDVSPDVFLSRLQAVIGVNTFVVGMLKAPF
                     MALVIGIIACIEGLAVQGSAESLGTHTTSSVVKSIFMVIVVDGLFAMFFAAIRY"
     gene            4696..5529
                     /locus_tag="PLQ11_08265"
     CDS             4696..5529
                     /locus_tag="PLQ11_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018042211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRK24933.1"
                     /translation="MTDPVAAPVPNANAANDAVIEVRDLTVAFGEKVIMNGLDLDVYR
                     GEILGFVGASGTGKSVLMRTILGLVQQKAGTIHVFGQDLATLSAFRRRAIERRWGVLF
                     QHGALFSSLTVKQNIQMPMREYLSMSEGLLDEMARLKIELVGLKPDAADKFPSELSGG
                     MIKRAALARALALDPEILFLDEPTSGLDPIGAQDFDDLIATLQRTLGLTVFMVTHDLD
                     SLAGVCDRIAALAEGKVIAQGAIEAMLTSEHPWLRAYFHGSRARRGSLASGQFRSSMK
                     A"
     gene            5533..6852
                     /locus_tag="PLQ11_08270"
     CDS             5533..6852
                     /locus_tag="PLQ11_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRK24934.1"
                     /translation="METRANYALVGLFTLAVLAACFGFVYWFRAANHGSERLSYRVVF
                     TGSVSGLSKGSSVRFNGLRVGEVTAVELLPDDLSRVAAYIEVDRTTPVRTDTRARLEY
                     QGLTGVASVHLSGVQSSAPPLTNPDGKGAPTIFADRSDFQDLFETAQRLAGRIDSIVT
                     RMDKILADGEAPFNSTLRNIEGFSRALNDNSPGMSAFLAQMTTTAQRFALLSDRFDRL
                     VEGADEILRGLDRQSLNRSLANFESFSKTLGDNKTQIEALVGDAASLAKRLNEAAPKL
                     DAALGDASSLMKSIDGARLNAMVADAEKFAKALGAKAGDVETALGQVAEITRKVNQSA
                     DRLDSVMKAAEDFLTDKQGRSVMVEIGETAKSIRTLANNLDKRTGEITAGINRFTGPG
                     LRELETLTSDARKAVNDVGRAVRSLERNPQQLITGGRSSIPEYSGSR"
     gene            6863..7456
                     /locus_tag="PLQ11_08275"
     CDS             6863..7456
                     /locus_tag="PLQ11_08275"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HRK24935.1"
                     /translation="MGGRRVVGQVARALTTAIAIAWLTACSSPPPATFDLGAAPRPAA
                     PMGRGQLAVAEPQAVTFLDSERMAVRTSGNTIAYLPGGQWADRLPKLVQARLIESFEN
                     ARRIRSVARPGGRIMAAHQLNSDIRTFEVQEASREAVVEITARIVNDRTGQIVAAEQF
                     AARAPVESIDAAGAAKALDAASQKVLADLVRWVSERA"
     gene            7453..7662
                     /locus_tag="PLQ11_08280"
     CDS             7453..7662
                     /locus_tag="PLQ11_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24936.1"
                     /translation="MMQVSPDLGSCRHCGRGPVHVSAQWCPHCGGVAPHVRMPPARNW
                     RPLLAILGLAAVLIAGVAALYFVGR"
     gene            complement(7852..>8267)
                     /locus_tag="PLQ11_08285"
     CDS             complement(7852..>8267)
                     /locus_tag="PLQ11_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018386964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24937.1"
                     /translation="AARPGQKGWLSDLLQRASTAEPAAQAPAADPLTLDIARMVDPEV
                     LEDAWDRYGRNEANPFTRRLYTVQGQQTFDEISRKYRRDQEFHETVDRYINEFERLLE
                     ETGDVTRSGKTARSYLSSDSGKVYTMLAHAAGRIG"
BASE COUNT         1450 a         2497 c         2876 g         1444 t
ORIGIN      
        1 gggtcgggat tgttcttgct ggcggtcgcg aacacgccgc cgatgcagcc gtaatcgacg
       61 agagccggat cgagcacggc aagatcggca ttgttcttga gggtcacgcc gatgatcgcc
      121 tccggtccga gcaggcggcg cgcatcggcg gggtgcatgt cgtcacggcc gaggtgtacg
      181 ccatcggctt cggcggcgag cgccacatcg atgcgatcat tgatgatgag cgggatgcgg
      241 ccggcaagcg ctgccttcac ctggcgggcg cgtgcgacaa aggagcgggt gccagcattc
      301 ttgtcgcgca gttgcagcag ggtggcgcca ccggcggcgg ctgcatccgc aagccggtca
      361 agcggacggc cgccggttcg ctcggggtcg agaatggcgt agaggcgaag atcgacgtgg
      421 ttcatgcaaa acggacccgt tcggcaagtg tggcaggatc aagggtggcg agcgcgtcga
      481 tcaggcggac ggcaaagctg ccggggccgg tcgcgccttc ggcggcgcgt tcggcggcga
      541 tgccataaag cgcgagcgcc gcagcggtcg cggtcatcgc atccggttca acggcgaggc
      601 aggcagcaat caccgcgccc agcgcgcagc cggtggcagt cacctgcccg gcgaggggat
      661 ggccgttggc aaggcgcagc gtgcggccgg cttgcgaaag ggtgtcaatg gcgccggtca
      721 ccactgtcac ggcggtggcg ggaatggcca gcaggctggc tgcctcggca cggttgagct
      781 tgacgaccgt ggggccggcc gcaaccaatt ccgcggcgag ggagcgacgc gggccggaga
      841 catcgacaaa caccggatcg agcacgaaag gcttgccgcc ctgccgcgcc agggcagcgg
      901 ccagcggggc cgtgtcgcga cgctcggcgt ccagcatgcc cagattgatc agcaaggcat
      961 cgcagccgcc gacaaagccg gcaatctcct cccggttggc cgtcagcgac ggacgcgcgc
     1021 ccaacgccag cagggtgttg gcggtgcgct ctgcggccac cgggctggtc aatgcatgga
     1081 cgcgcacgcg acccgtcctc aggcgcgtca gcacctctgc catggacagg atgatcggtt
     1141 cgttcgacat gccgtgcttt cctccgccgg aatgacccgg atcaggttct acgggttggc
     1201 atggtgcctc tcagcccgca tccatgacgg gcaccccgag cagctttcgg tccggagcgt
     1261 ccgacctgac cggatcaggt tggatgcgcc aggctattgg tttctcatca tctttttccg
     1321 ccagccggtg tccacttggc gggatgatgc tctagatcac gccgcatttt gatggaatca
     1381 tcaaaatgcg gataacgtga tcggtatcaa taagttagag catggcttct gcgattaacc
     1441 ggtatccact taatcgcgac atgctctagc taaggcgagc gcaacagaat tgcaaccggc
     1501 gatcacagcg gccgtgtcgg gctggcctca cgctggctgc gccgctcgat gccgagggcg
     1561 cgctcgcgca ggatgacaaa gatcccggag gcaatcacca ccgtagcgcc gatcagcacc
     1621 agccggtccg gtatctcgga gaacaccagc cagccgatca gcagcgccca gatcatcgag
     1681 gtatagtcaa acggcgcgat gatcgaggca tcggcatagc gatagcaatg ggtcagcagg
     1741 atctggccga tgccgccgag aaagccgatc atcgccagca tggcaaaatc gccggagggc
     1801 ggccagaccc aggcatggga gggccagaga tggccgaggc caaagctgac aagcgacagg
     1861 acggtcgtgg tcagcgagaa atagacgacg atggcgccgg tgtgttcggt ggcggtcagc
     1921 ttgcgcacct caatggtggc aaaggcggcg cagacagagg ccgcaagcgc cagcgccacg
     1981 cctttccagc tgccgctgct gccggccaga tgggttggcg acagatgcgg cgtcagcatg
     2041 atcagaacgc cgacaagccc gacgatcacc gccgtccagc gatagatgcg gatgacctcc
     2101 ttgagcagca accaggcgag caccaccgtc aacagcggcg tcgtgtagga cagggccgtg
     2161 gcatcgggca gcggcaggta ttgcaggccg gcaaagccca gaaacatgcc caaggcgccg
     2221 atcagaccgc gccttatgtg gcccctgagg ttagtggtga cgaaagcggc agacagcggt
     2281 cgggcatagg ccaaccacag caggaccggc agcagggcga acagcgagcg gaaaaacacc
     2341 agctcgccca acggaacgcg ggtgaaatcc ggtgcggtcg cgacatagcg gaccaggccg
     2401 gacatggcgg taaaagccag cgccgaggcg attttcagcc cgattccgaa cagtggccgg
     2461 gcggcaggct gttgcggggg cggcagggac gacggtgcgg tggtcacggg cggcaaaggg
     2521 cagagtgcga tcggaacagg cgggaatgat cgttatcaag agtttgtagc atgctctatc
     2581 gcagcaaagc gcctgtcggg gaaggaaaaa gctggccgga ccgcgctcag acaaacaggc
     2641 gcaaccgctc gtcgggtgtc agcatatcga gcaccgacag gccggagcgg cggggcggtg
     2701 ctccttcggt cgccgccggg cgtatatccg gatcggccag tatcgcatcg acggcggatt
     2761 gcacgccggg tgtatccggc caaagcgggc gcggctggtc gaaaaccgga gagtcctccg
     2821 cagcctgcgg gtatgccttg gcgcagcgct cgatgcccag gacatccagc agggtggcga
     2881 tgcgggtttc gacgaagcgg agggtttccg tcaccttgcg gatgcgctgg gcggcaatgt
     2941 cctgcgaggc gcaggccaga tagatctcgg tggcagaggc ttccagagcg tcacagtcga
     3001 gcggagcgcc gccgcgttcg cgcagcgtcc aggcctgctc ctgcatcttc tcggccgctg
     3061 tcaggatcga ggccgttgca gcttccgcag ccctgaccac cgcgtcaagt tctccactgg
     3121 ccgtaatgag gcggcccggg cagcccacgc cggggctgtg gcagccgatc tcgccctggg
     3181 tcgcggctat ggcggcggca atctcggtca gggccttgcg gaagggcagg gtatgatccc
     3241 ggtcggagag ggccttgacc tgccggtcca cccggtcgag cgcggccaga atggcagcag
     3301 cgtcgtcgcg gcgctggcgg ctggcgaatt cggcgaggaa ccagcgaccg cgctcggttt
     3361 gccttacggc agcctcgatg gcggcaaagt cgcgaaactg cggcatttca ggggatgacg
     3421 atgacatgac ggccgatccg cagactggga gagttgccgc agtttgatgg gttatggtta
     3481 atagccggtg caaagtgcca tttcttcagc gagtctgttc ccttgcctgc gcccctgacg
     3541 cccgaggccc agcgtctcac cctcaaaccc gatgccctgg tgtcgcggga aggggatttc
     3601 gtacgggtga cgctcagcgg cgattggtcg gccgccaatg cgcatgaatg cgaggatgag
     3661 gccgatgccg tgctgacggc ggtcaagggg gccagccggg tggtgatcga cgccagcggc
     3721 attgcccggc tcgatacgct ggggacatgg gtgctgtcgc gtgtcggcca tgatgtcatc
     3781 gagtccggat cgaggctggc ggtgacgggc gctagcccgg cgcatcggct gctgtttgat
     3841 gaggtgcagt atcgacgcca gcaggaaccg ccgccgccgc gcggcatcgt cggctttgat
     3901 atcctcagcg atatcggcga ggcgatggtg gatgcgttca gggatctggt caacggcatt
     3961 gcctttctcg gggcgatcgt cgccgcctgg gggcgcatcc tgaccggccg cagccggttc
     4021 cggctggcgg ccctggtcgg ccagcttgag cgggttgggc tgcgctcggt gccgatcatc
     4081 gtgctgattt cgctgctggt gggcggcatc attgcgcagc agtcggtgtt ccagctgcag
     4141 aaattcggaa ccgtgcattt cgtcgccgac gggctcggcg tgctggtgct gcgggagctg
     4201 gccgtgctga tcacggcgat catgatcgcc ggccgcaccg gttcgagctt taccgccgaa
     4261 ctcggctcga tgaagatgcg cgaggagatc gacgcgctgc gcacgatggg cctcaacccg
     4321 atcgaagtgc tggtcgtgcc acgcattctg gccctgctga tcggacttcc actgctgacc
     4381 ttcatcagtt cgatggcgat gctgtttggc ggcgggctgg tggcgatgat ctatggtgat
     4441 gtcagccctg atgtcttcct gtcccggttg caggcggtga tcggcgtcaa caccttcgtc
     4501 gtcggcatgc tgaaagcgcc gttcatggcg ctggtgatcg gcatcattgc ctgcatcgaa
     4561 ggactggcgg tgcaggggtc tgccgaatcg ctgggcacgc acaccacctc gtcggtggtc
     4621 aaatccatct tcatggtgat cgtggtggac gggctgttcg ccatgttctt cgccgcgatc
     4681 cggtattgag gcgccatgac cgaccctgtc gcagcccctg tgcccaatgc caatgccgcc
     4741 aacgatgcgg tgatcgaggt gcgggatctg acggtcgcct tcggcgaaaa ggtcatcatg
     4801 aacggcctcg atctcgacgt ctatcgcggc gaaattctcg gcttcgtcgg cgcgtccggc
     4861 acgggcaaat cggtgctgat gcgcaccatt ctcgggctgg tgcagcagaa ggccggaacg
     4921 atccatgtct tcggacagga tcttgctacg ctgtccgcgt ttcgccggcg ggccatcgag
     4981 cggcgctggg gcgtgctgtt ccagcacggg gcgctgtttt cctcgctgac ggtgaaacag
     5041 aatatccaga tgccgatgcg cgagtatctc agcatgtccg aggggctgct ggacgagatg
     5101 gcgcggttga aaatcgaact ggtcggcctc aagcccgatg cggcggacaa gtttccgtcg
     5161 gaattgtcgg gtggaatgat caagcgcgcc gcgctggccc gggcgctggc gctcgatccg
     5221 gaaatcctgt ttctggatga gccgacctcg gggctcgatc cgatcggtgc gcaggatttc
     5281 gacgatctga tcgcgaccct ccagcgcact ttggggctga ctgttttcat ggtaacccac
     5341 gatctcgact ctctggccgg cgtctgcgac cgcatcgccg cgctggccga aggcaaggtg
     5401 attgcgcagg gggccatcga ggcaatgctg acgtccgaac acccctggct gcgcgcctat
     5461 ttccatgggt cacgcgcccg ccgcggcagc cttgccagcg gccagttccg ctcctcgatg
     5521 aaggcctgag cgatggagac gcgcgccaat tacgctctgg tcgggctgtt caccctggcg
     5581 gtgctggcgg cgtgtttcgg ctttgtctac tggttccgcg ccgccaacca cggcagcgag
     5641 aggctcagct accgggtggt gttcaccggc tcggtgtccg gcctgtcgaa gggctcgtcg
     5701 gtgcgcttca acggcttgcg cgtcggcgag gtgacggcgg tcgaactgct gccggatgat
     5761 ctgagccgcg ttgccgccta tatcgaggtc gaccggacca cgccggtgcg gaccgatacc
     5821 cgcgcccggc tcgaatatca ggggctgacc ggcgttgcct cggtgcatct ttccggtgtt
     5881 cagagcagtg cgccgccgct gaccaatccg gacggcaagg gggcgccgac gattttcgcc
     5941 gatcgttccg atttccagga cctgttcgaa acggcgcagc ggctggccgg gcggatcgac
     6001 agcatcgtga cgcggatgga caagatcctg gccgatggcg aggcgccgtt caattcgacc
     6061 ttgcgcaaca tcgaggggtt cagccgcgcg ctcaacgaca attcgcccgg aatgtcggcg
     6121 tttctggcgc agatgaccac aacggcgcag cgttttgcgc tgctgtcgga ccggttcgac
     6181 cggctggtcg agggcgcgga tgagatcctg cgcgggcttg accggcagag cctcaatcgt
     6241 tcgctcgcca atttcgagag cttttccaag acgctcggcg acaacaagac gcagatcgag
     6301 gcgctggtcg gcgatgcggc gagcctggcc aaacgtctca acgaggcggc gccgaagctt
     6361 gatgccgcgc tgggcgatgc ctcgagcctg atgaaatcca tcgacggggc gcggctcaat
     6421 gccatggtgg cggatgccga aaaattcgcc aaggcgctgg gcgccaaggc cggcgatgtc
     6481 gagaccgcgc tggggcaggt tgccgagatc acccgcaagg tcaatcaatc ggccgatcgc
     6541 ctcgatagcg tcatgaaggc cgccgaggat ttcctgaccg acaagcaggg ccgcagcgtg
     6601 atggtggaga tcggggagac ggccaagtcg atccgcacgc tcgccaacaa tctcgacaag
     6661 cgcaccggcg agatcaccgc cggcatcaac cggttcaccg ggccgggctt gcgggaactc
     6721 gagacgctga cgagcgatgc gcgcaaggcc gtcaacgatg tcgggcgggc cgtccggtca
     6781 ctggaacgca atccacagca gttgatcacc ggcgggcggt cctctattcc ggaatacagc
     6841 ggcagtcgct gacggaatca tgatgggcgg gcgtcgagtg gtcggacagg tggcaagggc
     6901 gctcacgaca gcgatcgcta tcgcttggct gaccgcctgt tcgagcccgc cgccggcaac
     6961 cttcgatctt ggtgccgcgc caaggccggc tgcgccgatg gggcgggggc aattggcggt
     7021 cgccgaaccg caggccgtga cgtttctcga ttccgaacgg atggcggtgc gcacatccgg
     7081 caatacgatc gcctatcttc caggtggcca atgggcagat cgattgccca aactggtgca
     7141 ggcgcggctg atcgaaagtt tcgagaatgc gcggcgtatc cgcagtgtcg cccggccagg
     7201 cggacgcatc atggcggccc atcagctcaa cagcgatatc cgcaccttcg aggtgcagga
     7261 ggcaagccgt gaggcggtgg tggagatcac ggcgcgcatc gtcaatgacc ggaccggtca
     7321 aattgtcgcg gctgagcaat ttgcggcgcg cgcgccggtc gagtctatcg atgcagccgg
     7381 ggcggcaaag gcgctcgacg cggcgagcca gaaggttctg gccgatctgg tgcgctgggt
     7441 gtcggagcgg gcatgatgca agtcagtccg gatctgggct cctgccggca ttgtggacgc
     7501 ggacccgtgc atgtctcggc gcaatggtgc ccgcattgcg gcggcgtggc gccgcatgtg
     7561 cgcatgccgc cggcacgtaa ctggaggccg ctgctggcga tcctgggact tgcggccgtg
     7621 ctgatcgccg gggtggccgc gctctacttc gtcggccggt aagacgggct gcctgaaacc
     7681 ccgcgagggc attggagcaa gaaggccgat ctgactggat cgctcttttg ctcgaattta
     7741 ttccgaccgc atcatctttt ccgccagccg gtatccacct gacgggatga tgctctaaaa
     7801 agacggccgg ggattccatc ccaggccggc ttttcgttac cgtttcccgc gtcagccgat
     7861 gcggccggca gcatgggcga gcatggtgta gaccttgccg ctgtccgaac tcagatagct
     7921 gcgtgcggtc ttgcccgagc gtgtcacatc gccggtttcc tcgagcaggc gctcgaattc
     7981 gttgatgtaa cggtccaccg tttcatggaa ttcctgatcg cggcgatact tgcggctgat
     8041 ctcgtcaaag gtctgctggc cctgcacggt atagagccgg cgggtgaagg gattggcctc
     8101 gttgcggccg tagcgatccc aggcgtcctc aagaacctct ggatcgacca tgcgggcgat
     8161 gtcgagggtc aaaggatcgg cagccggagc ctgtgcggct ggctcggcgg tcgaggcgcg
     8221 ctgcaacaga tccgacagcc aacccttctg gccgggacgg gccgccg
//
LOCUS       DAOMXG010000108         8168 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_4598_length_8168_cov_10.001479, whole genome shotgun sequence.
ACCESSION   DAOMXG010000108 DAOMXG010000000
VERSION     DAOMXG010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 8168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8168
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4598_length_8168_cov_10.001479"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..367
                     /locus_tag="PLQ11_08290"
     CDS             65..367
                     /locus_tag="PLQ11_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24938.1"
                     /translation="MAARIGALVEAEIAGFDASSRAGAMTLQDFQARRVRRHRAVTVN
                     FSGGITQTCWTVCRSDGCYSVLYVPRAECFALCVDSDFGPLDIGVHGRALDCFASV"
     gene            565..1020
                     /locus_tag="PLQ11_08295"
     CDS             565..1020
                     /locus_tag="PLQ11_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24939.1"
                     /translation="MVSDICSAVGIDCDFIAMNGEIHNAIAYRGEFGERIIVYERRFS
                     PTIGYDGAQVVIAHEMGHHHCGHLSTRSGIGSHQVELEADAFMGFAMKMMSIPFESIQ
                     RTYELLGMGPESESHPSLTRRLAASRFGYQAGSLEDACNISINEGSKIA"
     gene            1017..2480
                     /gene="hydA"
                     /locus_tag="PLQ11_08300"
     CDS             1017..2480
                     /gene="hydA"
                     /locus_tag="PLQ11_08300"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018073738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HRK24940.1"
                     /translation="MSTVIKNGTIVTADLTYKADVLVEGGVITAIGTGLKGDRELDAT
                     GCYVMPGGIDPHTHLEMPFMGTYSSDDFESGTRAALAGGTTMVVDFALPSPGQGLLDA
                     LQMWDNKSTRANCDYSFHMAVTWWGEQVFNEMKTVIEERGINTFKHFMAYKGALMVND
                     DEMYASFQRLAELGGIAMVHAENGDIVAEMTAKLLAAGNTGPEAHAYSRPSQVEGEAT
                     NRAIMIADMAGVPLYVVHTSCEEAHEAIRRAKQNGKRVWGEPLIQHLTLDESEYFNPD
                     WDHAARRVMSPPFRNKKHQDSLWAGLQAGTLSVVATDHCAFTTEQKRYGLGDFSKIPN
                     GTGGLEDRMPMLWTHGVGTGRLTMNEFVAVTSTNIAKIMNMYPKKGAVLVGADADLVV
                     WDPAKEKTISAGTQQSAIDYNVFEGQRVKGLPRFTLTRGKVAVHDGEIRTEEGHGKFV
                     KREANGAVNRALSSWKDLTAPRPVARSGIPATGVAMV"
     gene            complement(2516..3010)
                     /locus_tag="PLQ11_08305"
                     /pseudo
     CDS             complement(2516..3010)
                     /locus_tag="PLQ11_08305"
                     /inference="COORDINATES: protein motif:HMM:NF004128.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
     gene            3260..3733
                     /locus_tag="PLQ11_08310"
     CDS             3260..3733
                     /locus_tag="PLQ11_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006727345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRK24941.1"
                     /translation="MATGKPVHHYRVTVTWAGNRGAGTQHYRAYGRDHLLAAAGKPDI
                     AGSADPAFLGDPARWNPEELLVGALSACHKLWYLHLCASHGISVTAYADEAEGSMIED
                     SDRGGWFTGVILRPLVTLRAGDDAALALSLHEAAHAKCFIANSVNFPVLVEAVVG"
     gene            complement(3755..4318)
                     /locus_tag="PLQ11_08315"
     CDS             complement(3755..4318)
                     /locus_tag="PLQ11_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019904861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24942.1"
                     /translation="MSSQRLDPADFASTPAPGRDCGSCTLCCKVFDVEALNKPMGQWC
                     PHCKPGRGCGIHETRPAHCRAYHCFWMLAPFLGPEWKPDRARFVLSIDPQSKFLMAQV
                     DPAQPTAWRQEPYYSQLKKWAVAGVSHGRQVLVFNGKAATAILPDRDVDLGVIAPDER
                     VFFTVHPVVGGFAFNAEKRKVGPQASA"
     gene            complement(4326..5270)
                     /locus_tag="PLQ11_08320"
     CDS             complement(4326..5270)
                     /locus_tag="PLQ11_08320"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HRK24943.1"
                     /translation="MSSKIQIVAGNSNRPLADAIAAHLQQPLTACQVRRFADMEIFVE
                     IQENVRGRDVFVLQSTSFPTNDHLMELLIITDALRRSSAKRITAVMPYFGYARQDRRS
                     SGRTPISAKLVANMIEKAGADRVLTLDLHAGQIQGFFDIPTDNLYAAPVMVRDVQERM
                     DCANVMVVSPDVGGVVRARALARRIGAPLAIVDKRRERPGESEVMNIIGDVKGRSCIL
                     VDDIVDSGGTLCNAADALLAHGAKDVYAYITHGVLSGGAVARITASKLKELVITDSIQ
                     PTEAVRVARNIRVLSIATLIGDAIARTAHEESVSSLFD"
     gene            complement(5370..5903)
                     /locus_tag="PLQ11_08325"
     CDS             complement(5370..5903)
                     /locus_tag="PLQ11_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24944.1"
                     /translation="MRRTGVFLMMAALLGLAACQDSGLDLAARSERKIVVGRNITLAM
                     VSLDGLPDTVTPRFSAALAEAAQARDMAFIDAADKPRFRLRGYLSAYSGEGGTSVSYV
                     WDVFDASLNRARRVAGSETLSRRDADPWAAMDEAALRRIAGRSLDGVGEFLISQPQES
                     APVAARPGLSNARAAAD"
     gene            complement(5997..7148)
                     /locus_tag="PLQ11_08330"
     CDS             complement(5997..7148)
                     /locus_tag="PLQ11_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006338506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HRK24945.1"
                     /translation="MALHFSPSEFSRRLRAVQAAMAAERLDAMLLFQQESMFWLTGYD
                     TFGFCFFQCLVVTADGRGALLTRSADLRQARHTSNIADIRIWKDAADANPAADLKALA
                     ADLGLAGRRIGVEYESYGLVAANGFKLGRAFAGFAELVDASHLVTRLRAIKSAEEITY
                     VRKAAQLCDLADAAAIAELGPGADEGAILAAQHNAIFAGGGDYPANEFIIGSGRDALL
                     CRYKAGRRKLSDNDQITLEFAGTCRHYHVAAMRTHVVGTPRPLHLTYHGVVKEALLAC
                     EAALKPGRTAGDVFSAHAKVFDSHGLGAHRLNACGYSLGAKFTPSWMDWPMFYENNPW
                     VLEPGMVMFIHMILMDSASETAMCLGRTYLVTETGSEALNLASLDLVVR"
     gene            7488..>8168
                     /locus_tag="PLQ11_08335"
     CDS             7488..>8168
                     /locus_tag="PLQ11_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24946.1"
                     /translation="MAGLRFRLDLGLGRLRGEPAEPQGAALAFPLAGVDPFVGYPSSI
                     GTVAPSGRAEWTVQNLFARVPDDLAALISPDPRDAQRTLAALVERLEFYRTKMAEYQE
                     RAARSGNSAVSIETYIRLVYIQLFSEIAGVFRLDLTREAYAQARADAFVRVIRQIKLM
                     TTADLIKVADLIQDEVLNAIIARSAWIASGDNGPAAPPPKAPQIILPQGTFEPNAAPR
                     PAATQAAEP"
BASE COUNT         1580 a         2441 c         2724 g         1423 t
ORIGIN      
        1 tccaccccct tgagaaaccc ccgaggatat ttctggaaca aagatggggc aaagggacga
       61 ggatatggcg gcgcggatcg gggcgctggt cgaggccgag atcgccggtt tcgacgcctc
      121 ctcgcgcgcg ggggcgatga ccttgcagga tttccaggcg cggcgggtca ggcggcatcg
      181 ggcggtgacg gtgaatttct cgggcgggat cacccagacc tgctggaccg tctgccggtc
      241 ggacgggtgt tattcggtcc tctatgtccc ccgcgcggaa tgcttcgcgc tgtgtgtgga
      301 cagcgatttc gggcccttgg atatcggcgt gcacgggcgg gcgctcgatt gcttcgcgtc
      361 ggtttgaaat ttgtgatggg gagcaggcct tgcatcaatg cgacaaacaa ggctgtcctg
      421 ggtggcacac tatttctggc gggaacgagt agattgtcag accaatcaat cgagatagat
      481 ctcggcgacg taacgcgaac aatgcacccg ctgccttcgg tcggcaggat ccccatcttt
      541 ccagcgtttg aagattactt gttcatggtc tcggatatct gttccgctgt cggtattgat
      601 tgcgacttca tcgctatgaa cggtgaaata cacaacgcca tcgcttatcg tggggaattt
      661 ggcgagcgga tcatcgtata cgagcggcgg ttcagcccaa ccattggata cgatggtgct
      721 caggtcgtaa ttgcgcacga aatggggcat catcattgcg gccatctttc aaccagaagc
      781 gggatcgggt ctcatcaagt cgaactcgag gccgatgcgt ttatgggctt cgcgatgaaa
      841 atgatgagca ttcccttcga aagcatccag cgaacttacg agcttttggg tatggggccg
      901 gaatcagaaa gtcatccgtc actcacaaga agactcgctg catctagatt cggctatcaa
      961 gctggttcct tggaagatgc gtgcaatatt tcaatcaacg aggggtcaaa aatagcatga
     1021 gcaccgtcat caagaacggc accatcgtca ccgcggatct gacctacaag gccgatgtgc
     1081 tggtggaggg cggggtgatc accgccatcg ggacggggct gaagggcgac agggagctgg
     1141 acgcgaccgg ctgctacgtc atgccgggcg ggatcgaccc gcatacccat ctggagatgc
     1201 ccttcatggg cacctattcc agcgatgatt tcgagagcgg aacgcgggcg gcgctggcgg
     1261 gcggcaccac gatggtcgtc gatttcgcgc tgccgagccc ggggcagggg ctgctcgacg
     1321 cgctgcagat gtgggacaac aaatcgaccc gggccaattg cgactacagc ttccacatgg
     1381 cggtgacctg gtggggcgag caggtcttca acgagatgaa gaccgtgatc gaggaacgcg
     1441 gcatcaacac cttcaagcat ttcatggcct acaagggcgc cttgatggtg aacgatgacg
     1501 agatgtatgc ctcgttccag cggctggcgg aactcggcgg gatcgccatg gtccatgccg
     1561 agaacggcga tatcgtcgcc gagatgacgg ccaagctgct ggccgcgggc aacaccgggc
     1621 ccgaggcgca tgcctattcg cggcccagcc aggtcgaagg cgaggcgacc aaccgcgcca
     1681 tcatgatcgc cgacatggcg ggtgtgccgc tctatgtggt gcatacctcc tgcgaggagg
     1741 cgcatgaggc gatccggcgg gccaagcaga acggcaaaag ggtctggggc gagccgctga
     1801 tccagcatct gacgctggat gagagcgagt atttcaaccc cgattgggac catgcggcgc
     1861 ggcgggtcat gtcgccgcct ttccgcaaca agaagcatca ggacagcctc tgggccgggt
     1921 tgcaggccgg cacgctgtcg gtggtggcca ccgaccattg tgccttcact accgagcaga
     1981 aacgctatgg cttgggcgat ttctcgaaga tcccgaacgg gaccgggggg cttgaggacc
     2041 ggatgccgat gctctggacc catggcgtcg ggaccgggcg gctgacgatg aacgaattcg
     2101 tggccgtgac atcgaccaat atcgccaaga tcatgaacat gtatccgaag aagggcgcgg
     2161 ttctggtcgg cgccgatgcg gatctggtgg tctgggatcc ggccaaggag aagacgatca
     2221 gcgcggggac gcagcaatcg gccattgatt acaatgtctt cgagggccag agggtcaagg
     2281 gcctgccgcg tttcaccctg acccggggca aggtcgccgt gcatgacggc gagatccgca
     2341 ccgaggaggg gcatggcaaa ttcgtgaagc gcgaggccaa tggcgcggtc aaccgcgcgc
     2401 tgtcgagctg gaaggacctg accgcgcccc ggccggtggc gcggtcgggc atcccggcga
     2461 cgggggtggc gatggtgtaa tcgccatcct ctgccgccct ggcaccttcg ggcagcttga
     2521 tgttctcaag atgcacggaa tcgttgatgt cgaggccggc aaggtcggcg gtgatgaatt
     2581 ccgggatcgc atcgcccggg acgttcattt ccaccgaatg ggcgacgata ttgagcgcac
     2641 cgccgcgctt gaggcccgga gacgcctcgt ggccggtgaa atgcacgggc acctcgaccc
     2701 aaacgcggct gccgacgcca aggcgcagga agtcgatatg caccggcctg tcgcgcaccg
     2761 gatcaagctg gtagtcgcgc ggaatggcgc ggaacttctg accatcgacg tcgatttcaa
     2821 aaaccgtggt gaggaagcgg ccggcataga tgagccggtt ggcaaccgta taatcgagcg
     2881 aaatcgacac agggggctcg ccggcgccgt agacgacggc cggaacgcgg ccctcgcgac
     2941 gaacagcccg ggcggccccc ttgccggcct tctcgcggcg cttggccgag agctgcttga
     3001 cgttggacat gatagtttcc ttttgttgac tatggacggc gcgggcgatc tgctcgcaca
     3061 cgaccatccc ggtgcctcca ggggtgtctg gccgggacgg cgctccttag ggtaaaacag
     3121 cgccgagggc aaggcttaga gcatcatccc gccagatgga tcccggctgg cggaaaaaga
     3181 tgatgcgttc agaatagatt ggagcaaaag agcgatccag tcagatcggc ttttgctcca
     3241 gcgcaacagg aggagacgga tggccaccgg caaaccagtt catcattatc gcgtcacggt
     3301 gacatgggcc ggcaatcgcg gcgccggcac gcagcactac cgcgcctatg gacgcgatca
     3361 tctgctggcc gcggccggca agccggatat cgcgggctcg gccgatccgg cctttctggg
     3421 cgatccggcc cggtggaatc cggaagagct gctggtcggg gcgctctcgg cctgccacaa
     3481 gctgtggtat ctgcacctct gcgccagcca cggcatttcg gtgacggcct atgccgacga
     3541 ggccgaaggt agcatgatcg aggacagcga tcgcggcggc tggttcaccg gcgtcatctt
     3601 gcgtccgctt gtcacgcttc gcgccggtga cgatgcagcg ctggccctca gcctgcacga
     3661 ggcggcgcat gccaaatgct tcatcgccaa ttcggtgaat tttccggtgc tggtcgaggc
     3721 ggtcgtcggc tgaggccggc tacccacgag gcgatcaggc gcttgcttgc ggcccgacct
     3781 tgcgcttctc cgcattgaag gcgaagccgc cgaccaccgg atgcacggtg aaaaacaccc
     3841 gctcgtcggg cgcgatgacg ccaaggtcga catcccggtc aggcaggatg gcggtggcgg
     3901 ccttgccgtt gaacaccaga acctgccgcc cgtgcgatac tccggcaacc gcccatttct
     3961 tcaactggct gtaatagggc tcctggcgcc aggccgtcgg ctgggccgga tcaacctgcg
     4021 ccatcagaaa cttcgactgc ggatcgatcg acagaacgaa gcgggcgcgg tccggtttcc
     4081 attcaggccc aaggaacggg gcgagcatcc agaaacaatg ataggcacgg caatgggccg
     4141 ggcgggtctc atgaatgccg cagcccctgc cgggtttgca atgcgggcac cattgcccca
     4201 tcggcttgtt cagcgcctcg acatcgaaca ccttgcagca cagggtgcag gaaccgcagt
     4261 cacgcccagg ggccggggtc gaggcaaaat cggcaggatc gaggcgctgg ctgctcatct
     4321 ctggctcaat cgaacaggct ggagaccgat tcctcatgcg cggtgcgggc tatcgcgtcg
     4381 ccgatcagcg tcgcgatcga caggacgcgg atgttgcggg caacgcgcac cgcctcggtc
     4441 ggctggatcg aatcggtgat gacgagttcc ttgagcttgg aggcggtgat gcgtgccacc
     4501 gcgccgcccg acaacacgcc atgggtgata taggcataga cgtccttggc gccgtgggcg
     4561 agcagcgcat cggcggcgtt gcacagggtg ccgccggaat cgacgatgtc atcgaccagg
     4621 atgcaggagc ggcccttcac atcgccgatg atgttcatca cctcgctctc gcccggtcgc
     4681 tcgcggcgct tgtcaacaat ggcgagcggc gctccgatgc ggcgggccag cgcgcgggcg
     4741 cgcaccacgc cgccgacgtc cggcgagacg accatgacgt tggcgcaatc catccgttcc
     4801 tgcacgtcgc gcaccatgac cggcgccgcg tagagattgt cggtcggaat gtcgaagaag
     4861 ccctgaatct ggccggcgtg cagatcaagc gtcaaaacac gatccgcccc ggccttctcg
     4921 atcatgttgg caaccagctt ggccgagatc ggcgtgcggc ccgacgagcg gcgatcctgc
     4981 cgcgcatagc cgaaataggg catcaccgcg gtgatccgct tggccgagga acggcgcagc
     5041 gcatcggtga tgatcagaag ttccatcaga tggtcgttgg tcgggaatga cgtcgattgc
     5101 agcacgaaca cgtcgcgacc gcgcacattc tcctggatct cgacgaaaat ctccatgtcg
     5161 gcgaagcggc gcacctgaca ggcggtgagc ggctgctgga ggtgcgcggc gatcgcatcc
     5221 gccagcggcc ggttggaatt gcccgcgacg atctggatct tggacgacat gaacagaacc
     5281 cctgatgatg cagccgccat cgggactcgg cagttgcata gccgcgctcc ctttagagca
     5341 gtttgatttg atacggaagc gcctctgttt caatcggcgg cggcgcgcgc attggacagg
     5401 ccggggcggg ccgccaccgg cgccgattcc tgcggctggg atatcaggaa ctctccaacg
     5461 ccgtcgaggc tgcggccggc aatgcggcgc agcgcagcct cgtccatcgc cgcccagggg
     5521 tcggcatcgc ggcggctcaa ggtctcgctg ccggcgacgc ggcgggcccg attgagcgag
     5581 gcgtcaaaaa catcccagac atagctgacg gacgtgccgc cttcgccgga ataggcgctc
     5641 agatagccgc gcaggcggaa gcgcggcttg tcggcggcgt cgatgaaggc catgtcgcga
     5701 gcctgggctg cttccgccag ggcagcggaa aagcgcggcg tcaccgtgtc cggcaggcca
     5761 tccagcgaga ccatcgccag cgtgatgttg cggccgacga cgatcttgcg ctcggatcgt
     5821 gcggccagat caaggccgga atcctgacag gcggcaaggc caagcaacgc cgccatcatc
     5881 agaaaaacac cggtccgccg catcgccgtc tctacccgca caagccgtta acgctttctt
     5941 tacccttcgg gcgaagggag gggaagaggc agatccggcc ttccgcgcgg aatggtttac
     6001 cgcaccacca gatcgagcga ggcaaggttg agcgcttcgg acccggtctc ggtgacaaga
     6061 taggtgcggc cgaggcacat ggcggtttcg ctggccgaat ccatcaggat catgtggatg
     6121 aacatcacca tgcccggttc cagcacccag ggattgttct cgtagaacat cggccagtcc
     6181 atccagctcg gcgtgaactt ggcgcccagc gaatagccgc aggcattgag acgatgggcg
     6241 ccgaggccgt gggaatcgaa aaccttggca tgggcggaaa acacatcccc ggcggtgcgg
     6301 ccgggcttca gcgccgcctc gcaggccagc agcgcctcct tgaccacgcc atggtaggtc
     6361 agatgcagcg ggcgcggtgt gccgacgaca tgggtgcgca tcgccgcgac atggtagtgg
     6421 cggcaggtgc cggcaaattc gagcgtgatc tggtcattgt cggagagttt gcgccggccg
     6481 gccttgtagc ggcagagcag cgcgtcacga ccggagccga tgatgaattc attggccggg
     6541 taatcaccgc caccggcaaa aatggcgttg tgctgcgcgg ccaggatggc gccttcatcg
     6601 gcaccgggcc caagttcggc aatggcggca gcatcagcca gatcgcagag ttgtgccgcc
     6661 ttgcggacgt aggtgatttc ctcggccgat ttgatcgccc gcaggcgcgt gaccagatgc
     6721 gaggcatcga ccagttcggc aaatccggca aaggcccggc ccagcttgaa cccattggcg
     6781 gcaaccagac catagctttc gtattcgacg ccgatgcgcc ggccggcaag gccgagatca
     6841 gcggcaaggg ctttgagatc ggcggcggga ttggcatccg ccgcatcttt ccagatgcgg
     6901 atatcggcga tgttcgaggt atggcgtgcc tggcgcaggt ctgcggagcg ggtgagcaac
     6961 gcgccgcggc catcagcggt caccacaagg cactggaaga agcagaagcc gaaggtgtcg
     7021 tagccggtca gccagaacat gctttcctgc tgaaacagca gcatcgcatc gagacgctcg
     7081 gcggccatcg cggcctgcac ggccctgaga cggcgggaga attcggaagg cgagaaatgc
     7141 aaggccatga tgctcggtcc ccgttgcctc gggcaaggat agaccagcaa acaggccagg
     7201 caaaaagaaa acgccccgcc gctcctgagg agctgacggg gcggacagaa atccaggctt
     7261 tgggggcgat gtgggcctgg atctcgagtc tgtagttccg gaccggccga agccggggtc
     7321 acaccggatt gtttctacat aaacgacagg tcccgtcctg gttgtgcgct ggcgcacagg
     7381 atagaagcga tgtggcattg gccagcgctt aaccatgccc gttcagtgaa tgtttgcagg
     7441 gatgccgtaa cgtcgccgcg acagttgaca atgtggtgga gttcatcgtg gcgggcttgc
     7501 ggtttcggct ggatcttgga cttggcaggt tgcgtggcga accggcggag ccgcagggcg
     7561 ccgcgctggc gttccctctt gccggtgttg atccatttgt cggttacccg agcagcatcg
     7621 gcacggtcgc gccgtccggc cgggctgaat ggacggtcca gaacctgttt gcccgcgtgc
     7681 ccgacgatct tgccgcgctg atctcacccg atccgcgcga tgcgcagcgc acccttgccg
     7741 cattggtcga acgcctcgaa ttctaccgga ccaagatggc cgagtatcag gagcgggcgg
     7801 cgcggagcgg caacagcgcg gtatcgatcg agacctatat caggctggtc tatatccagc
     7861 tgttcagcga aatcgccggg gtgtttcgtc ttgacctgac gcgggaggct tacgcccagg
     7921 cgcgggcgga cgcgttcgtc cgcgtcatcc ggcaaatcaa gctgatgacc acggcggatc
     7981 tgatcaaggt ggccgatctc attcaggacg aggtgctgaa cgcgatcatc gcccgctcgg
     8041 catggatcgc aagcggagac aatggcccgg cggcgccccc tcccaaggcc ccgcagatca
     8101 ttctgccgca gggaaccttt gagccgaatg ccgcgccccg ccctgcggcg acccaggcag
     8161 cggagccc
//
LOCUS       DAOMXG010000109         8070 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_4668_length_8070_cov_9.938989, whole genome shotgun sequence.
ACCESSION   DAOMXG010000109 DAOMXG010000000
VERSION     DAOMXG010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 8070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8070
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4668_length_8070_cov_9.938989"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /locus_tag="PLQ11_08340"
     CDS             <1..859
                     /locus_tag="PLQ11_08340"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRK24947.1"
                     /translation="WDWKSGSYLPDHVCVQERLAEEKDADAFDRPRTRRRVERVKRQF
                     EALRPRRRIVRRQTDGFDLDFDEVVRARCDLIASGEASDRLYQAIRDDERDLAVSVLI
                     DVSRSTESSVGGRPVIEIAREALAALIGGIDACGDAVSVHAFSSLRRDRVMVEKVKDF
                     GEDNNELIRRRIMGLTPRFYTRMGAAIRHVSRHLAGHTAQRRLLLVITDGKPNDLDHY
                     EGRHGIEDTRRAVQEARRLGQAVFAITVDARARDYIPYLFGRNGFAMVPDAEKLIEAL
                     PDMYRHVAG"
     gene            846..1412
                     /locus_tag="PLQ11_08345"
     CDS             846..1412
                     /locus_tag="PLQ11_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HRK24948.1"
                     /translation="MSQADGRAAGDPFAGLPGHPMMWILIGSELLVFGAALIAFSAAR
                     LQDPAGFAEAQNHLDRLTGLINVLVLVTSGLFAALAVGAARIGNIPRTRLHLALASLL
                     GLVFLGFKGVEYSSDLAAGFTIDSGTFFTLYYLITGFHALHVVLGLVIFAVVFAWPSH
                     FNVETGAAFWHMVDLVWLLVFPVIYLVR"
     gene            1412..1687
                     /locus_tag="PLQ11_08350"
     CDS             1412..1687
                     /locus_tag="PLQ11_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HRK24949.1"
                     /translation="MDDDAKRLLRAWLIMAGLTLAAIPVGHATTMRPLGAGLTLLLLG
                     LAFVKSALLLHDYLDLRHAPRWNAGLRSGILVLLGIIVGLSLAAGLA"
     gene            complement(1691..2401)
                     /locus_tag="PLQ11_08355"
     CDS             complement(1691..2401)
                     /locus_tag="PLQ11_08355"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRK24950.1"
                     /translation="MAIPIEPFELKALRATAMFRSLNPRVAEKVLAGSVVEVHMPGSR
                     LFRQHEPAQALFLVLDGWVKIVREAPNGIPTVIGVFTRGQTLADAIAIAGGCYPVSAE
                     TVTRARLVRIDATAIRAIIHREPDIALAMIASTAQHLFGLMQQIEQLKALKSPERVAE
                     FLVSLTDISEGPVSIALPFDKSLIAARLGMQPESLSRAFLRLRDIGVRVEGAEVTISD
                     IAQVSAFAHSAEAAGTQH"
     gene            complement(2479..2730)
                     /locus_tag="PLQ11_08360"
     CDS             complement(2479..2730)
                     /locus_tag="PLQ11_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24951.1"
                     /translation="MKAQRSFQSNCVFDPSLCPLLAGLSPEDQQARMASDPMIRACVQ
                     SIAEGKAYPERCAMIPGKPDKTAESARKRMRRTRIAARA"
     gene            complement(2727..3200)
                     /locus_tag="PLQ11_08365"
     CDS             complement(2727..3200)
                     /locus_tag="PLQ11_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24952.1"
                     /translation="MRAELPLPTGQAANRRIIKGALVLVAMLAIPVVGLMLLQAGVLL
                     ACSTETVAEGVAEGRIEWRITRMQCRNGAEPFYDVALGAEDKTLSTALTSRGAPVPTG
                     VIRVDEGLVGIRLDRPRVGTAEDVVVVRLRRSGSPQQRIDLQADMPRANNRGAGS"
     gene            complement(3197..3619)
                     /locus_tag="PLQ11_08370"
     CDS             complement(3197..3619)
                     /locus_tag="PLQ11_08370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin family protein"
                     /protein_id="HRK24953.1"
                     /translation="MLPPSIPLGVTLMDDDHARIEDALAQAARVADAGLHAALQRCRA
                     ELAGHFAREEALMEAAGLPVLGCHVAQHRRLIDDVDAMLKDAAATPERLRLYIARDLP
                     NLVLAHIASIDQLSARFLTGEIPGATMARLRMAEEAAS"
     gene            complement(3631..5115)
                     /locus_tag="PLQ11_08375"
     CDS             complement(3631..5115)
                     /locus_tag="PLQ11_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008549343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HRK24954.1"
                     /translation="MKRLSELLLALVCLPLLATSAAAADAEQLYQTHCVACHGTGRLG
                     AIGPALLPENMGRLRGPAITKVIAEGREATQMPGFGQTLAKDEIEALTAYISRPLPQM
                     PAWGPMEISASRSIERAAPALAKPVFSADPLNLFVVVEIGDHHATILDGDKLEPLTRF
                     PTRFALHGGPKFSPDGRFVYFMSRDGWVEKYDIWSLQKIGEVRAGINARNIALSTDGK
                     TIAVANYLPNSLVLLNAEDLSLIRIFDAVDTAGKSSRVSAVYQARPRDSFVVALKDVA
                     EIWEIPTKSGDFPLRRIKTGEPLDDFFFDPSYAHVFGSAREGAASRVYNLDRGVQVAE
                     MALPGLPHLGSGITFKHDGRPLMATPHLRSGLVSIIAMDEWKTVKTVTTPGPGFFMRS
                     HENSPFAWTDSMMSKAKDTMSILDKRSLEIVRSITPSPGKTAAHVEFDRYGKQVLVSI
                     WEDPGELIIYDAQSFAEIKRMPMRKPSGKYNVYNKITFSEGTSH"
     gene            complement(5099..6328)
                     /gene="nirJ"
                     /locus_tag="PLQ11_08380"
     CDS             complement(5099..6328)
                     /gene="nirJ"
                     /locus_tag="PLQ11_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme d1 biosynthesis radical SAM protein NirJ"
                     /protein_id="HRK24955.1"
                     /translation="MFRLSHYLRTVLKPGQSAHAAGPKGNAPPLRMTGPVVIWNLIRR
                     CNLTCMHCYSISGDVDFPGELSTAQVFETLDDLKAFGVPVLILSGGEPLLRRDIFEIS
                     KRAKALGFFLCLSTNGTLIDAWMADRIAEADYDYVGISIDGLKETHDVVRRKAGAFDQ
                     SVEALRLLRDRGVKVGVRFTMTQENERHLEPLIDLCEAERFPKFYLSHLVYAGRGNKN
                     RGRDSEWASTRRAMDLMIARAVRAIDEGRDLEIVTGNNDADAVYLLHWVRAHHPDQAE
                     RLERMLVRWGGNASGVNVANIDNLGQVHPDTMWWHHTLGNVKERAFSDIWTDLSDPVF
                     AGLKQRPRAVTGRCGACAHLAICGGNTRIRALKVTGDPWAEDPGCYLSDAEIGLDNAA
                     PRITVTPFRGVSHEASV"
     gene            complement(6339..6827)
                     /locus_tag="PLQ11_08385"
     CDS             complement(6339..6827)
                     /locus_tag="PLQ11_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006938166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRK24956.1"
                     /translation="MLSASDQALIKATERGLPLTPDPYGDLARALDRSRAVIMERLGA
                     LLEIGAIRRIGVIPNHYALGVIANGMSVWDIDDSRVAELGPRIGALPFVSHCYRRPRH
                     LPAWRYNVFAMVHGRSRDEVHQKVGEIAALAGAGARAHDVLFSSRILKKTGLRLTGQP
                     AA"
     gene            complement(6830..7282)
                     /locus_tag="PLQ11_08390"
     CDS             complement(6830..7282)
                     /locus_tag="PLQ11_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006938167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRK24957.1"
                     /translation="MITLTDADRVIINGLQGGFPVSERPFAEAGARLGLSETELIARI
                     GALVEAGAISRFGPLWNSEELGGAVCLAAMSVPSERFDEVTAKVNAHAEIAHNYERDH
                     ALNMWFVLSSLDPARIRATARAIEEETGLKVWLMPKEEEFFVGFRVEV"
     gene            complement(7279..>8070)
                     /locus_tag="PLQ11_08395"
     CDS             complement(7279..>8070)
                     /locus_tag="PLQ11_08395"
                     /inference="COORDINATES: protein motif:HMM:NF037131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HRK24958.1"
                     /translation="GASTLAAVAARPERIEAVAAVINGFAGVNHNYERENAYNLWFVV
                     TGRDWADVTATLAGIEQQTGLAVLNLPLEKSYHIDLGFSLYGNRATKPVQKPLIGKAD
                     AEDRRLLGAIEQGLMLVPRPFAAVAERLHTSESAVIDRLAAMIERGIITRFGIIVRHR
                     AFGYSANAMAVWDVAEAAVDQVADGFAAAPGVTLCYRRPRRLPHWRFNLFTMIHGRDR
                     ASAMAVVDGLAQQAAPHLAGHSVLFSLRCFKQRGAVFSRSREAAE"
BASE COUNT         1354 a         2573 c         2691 g         1452 t
ORIGIN      
        1 atgggactgg aagagcggaa gttatctgcc cgatcacgtc tgcgtgcagg agcgccttgc
       61 agaagagaag gacgccgacg cgttcgacag accgcggacg cgccgccgtg ttgaacgcgt
      121 caagcggcag ttcgaggcgc tgcggccgcg ccgccgcatc gttcgccgcc agacggacgg
      181 gttcgatctc gatttcgacg aggtggtgcg cgcgcgctgc gatctgatcg cgtcggggga
      241 ggccagtgac cggctctacc aggcgatccg cgacgacgag cgtgatctgg cggtgagcgt
      301 gctgatcgac gtttcccgtt cgaccgaaag ctcggttggc ggccggccgg tcatcgagat
      361 tgcccgcgag gcgctggcgg cgctgatcgg cggcatcgac gcctgcggtg atgcggtttc
      421 ggtgcatgca ttctcgtcgc tgcgccgcga ccgggtgatg gtcgagaagg tcaaggattt
      481 cggcgaggac aacaacgagt tgatccgccg ccgcatcatg ggcctgacgc cgagattcta
      541 cacgcgcatg ggcgcggcga tccgtcatgt ctcccggcat ctggcgggcc atacggcgca
      601 gcgccggctg ctcctggtca tcactgacgg caagcccaac gatctcgacc attacgaggg
      661 tcgccacggc atcgaggata cccgccgcgc cgtgcaggaa gcccggcggc tggggcaggc
      721 ggtgttcgcc atcaccgtcg atgcgcgggc ccgtgactac atcccctatc tcttcggccg
      781 caatggtttt gccatggttc ccgatgccga gaagctgatc gaggccctgc cggacatgta
      841 tcgccatgtc gcaggctgac gggcgggcgg cgggcgatcc ctttgcggga ttgccgggcc
      901 atccgatgat gtggatcctg atcgggtccg aattgctggt gttcggcgct gcgctgatcg
      961 ccttttctgc ggctcggctt caggatccgg cggggtttgc cgaggcgcag aaccatctcg
     1021 accgcctgac cgggctgatc aacgtgcttg ttctggtgac cagcgggctg ttcgcggccc
     1081 tggccgtcgg cgccgcgcgg atcggaaaca tcccgcgcac ccgcctgcac ctggcgctgg
     1141 ccagcctgct tggcctggtg tttctcggct tcaagggggt cgaatactcg tccgatctgg
     1201 cggcgggttt caccatcgac agcgggacat tcttcacgct ctactatctg atcaccggct
     1261 ttcacgccct gcatgtcgtg ctggggcttg tcattttcgc ggtggtgttc gcctggccca
     1321 gccatttcaa tgtcgagacc ggagccgcgt tctggcatat ggtcgatctg gtctggctgc
     1381 tggtgttccc ggtcatctat ctggtgcgct gatggacgac gatgcgaaac ggctgctccg
     1441 cgcctggctg atcatggccg ggctgacgct tgccgccatt ccggtcggcc atgcgaccac
     1501 aatgcgcccg ctcggcgccg ggctgacatt gctgctgctc gggcttgcct tcgtcaaatc
     1561 ggcgctgctg ctgcatgact atctcgatct gcgccatgcc ccacgctgga acgccgggtt
     1621 gaggagcggc atccttgtgc tgctggggat catcgtcggc ctgtcgctgg ccgccggact
     1681 ggcctgatcg tcagtgctgc gtgccggccg cctccgccga atgggcgaag gcggagacct
     1741 gtgcgatatc cgagatcgtc acttcggcgc cttcgaccct gacgccgatg tcgcgcagcc
     1801 gcaggaaggc gcgcgacagc gattccggct gcatgcccag ccttgccgcg atcagcgatt
     1861 tgtcgaaagg cagggcgatc gataccggcc cttcgctgat atcggtcagg gacacgagaa
     1921 actcggccac ccgttcgggc gatttcagcg ccttgagctg ttcgatctgc tgcatcaggc
     1981 cgaacaggtg ctgtgcggtc gaggcgatca tcgccagcgc gatatccggt tcgcggtgga
     2041 tgattgcacg gattgccgtg gcgtcgatcc gcacaagccg ggcgcgagtc accgtctcgg
     2101 cagaaaccgg gtagcagccg ccggcaatgg cgatggcatc ggccagcgtc tggccgcggg
     2161 tgaatacgcc gatcaccgtc gggatcccgt tcggcgcttc ccgcacgatc ttgacccagc
     2221 cgtcgagcac cagaaacagg gcttgggccg gctcatgctg ccggaacagc cggctgccgg
     2281 gcatgtgcac ctcgaccacg cttccggcga gaaccttttc ggcgacacgc gggttcaggc
     2341 tgcggaacat ggccgtcgcc ctgagcgcct tcagttcgaa tggttcaatc gggattgcca
     2401 taccgtgtcc ccccgatggt gtgttctgtc ttgttgccag atggtcgtta caaagcccgg
     2461 ggccaacctt gaaatcggtc aagcccgggc ggctattcgt gtccggcgca tccgcttgcg
     2521 ggcggattcc gctgtcttgt ccggcttgcc cggtatcatg gcgcagcgtt ccggatatgc
     2581 tttgccctcc gcaatggatt gcacgcaggc ccggatcatc ggatcgcttg ccatgcgcgc
     2641 ctgctggtcc tcgggggaga ggccggcgag cagcgggcag agactggggt cgaacacgca
     2701 gttcgactgg aatgatcgct gggccttcat gagcctgctc cacgattgtt ggcccggggc
     2761 atgtccgcct gcaggtctat ccgttgctgc ggcgagccgg agcggcgcag gcgcaccacc
     2821 accacatctt cggctgtccc gacgcggggg cgatcgaggc ggatgccgac cagcccctcg
     2881 tccaccctga tgaccccggt cggcaccggc gccccgcgcg aggtcagcgc ggtcgacaag
     2941 gtcttgtcct ctgccccgag ggcgacatcg tagaagggtt ctgcgccatt gcgacattgc
     3001 atgcgggtga tgcgccattc gatgcgcccc tccgcgaccc cttcggccac cgtctccgta
     3061 gaacaggcca gcagcacgcc ggcctgcaac agcatcagcc cgaccaccgg tatggcgagc
     3121 atcgccacca gcaccagcgc ccccttgatg atgcggcggt tggcggcctg tccggtcgga
     3181 agcggcaatt cggctctcat gatgcagcct cctctgccat gcgaaggcgg gccatcgtgg
     3241 cgccgggaat ctccccggtg aggaaccggg ccgaaagctg gtcgatgctg gcaatatggg
     3301 caaggaccag gttcggcagg tcgcgggcga tgtaaagccg cagtcgctcg ggcgtggctg
     3361 cggcgtcctt gagcattgcg tcaacatcgt cgatcagccg ccgatgctgg gcgacatggc
     3421 agcccagaac cggcaggccg gcggcctcca tcaaggcttc ctcgcgggca aaatgtccgg
     3481 ccagttcggc ccggcaccgt tgcagcgccg catgcaatcc ggcatcggca acacgcgccg
     3541 cctgcgccag cgcgtcctcg atcctggcgt ggtcgtcatc catcagggta accccgagcg
     3601 gaatggaggg cggcagcatg ggcgcctcgt tcaatggctg gtgccttcgg agaaggtgat
     3661 cttgttgtag acgttgtatt tgccggaagg cttgcgcatc ggcatgcgct tgatctcggc
     3721 gaagctctgg gcgtcgtaga tgatcagttc gccgggatcc tcccagatcg agaccagcac
     3781 ctgcttgcca taacggtcga actccacatg ggcggcggtc ttgccggggc ttggtgtgat
     3841 cgagcggacg atctccaggc tgcgcttgtc gaggatcgac atcgtgtcct tggccttgga
     3901 catcatcgaa tcggtccagg cgaagggtga gttctcgtgg ctgcgcatga agaagccggg
     3961 accgggcgtc gtcaccgtct tgaccgtttt ccattcatcc atggcgatga tcgagacgag
     4021 acccgagcgc agatgcggcg tggccatcag cgggcggcca tcatgcttga aggtgatgcc
     4081 ggagcccaga tgcggcaggc cgggcagggc catttccgcc acctgcacac cccggtcgag
     4141 attgtagacc ctggaggcgg cgccctcgcg cgccgagccg aacacatggg cataggaggg
     4201 atcaaagaag aaatcgtcga ggggctcgcc ggtcttgatc cgccgcagcg ggaaatcgcc
     4261 ggattttgtc ggaatttccc agatctccgc aacgtccttc agcgccacga cgaaactgtc
     4321 gcgcgggcgg gcctgataca ccgccgagac gcgcgacgac ttgccggcgg tgtccactgc
     4381 atcgaaaatg cggatcagcg acagatcctc ggcattcagc agcacgaggc tgttgggcag
     4441 gtaattggcc accgcgatgg tcttgccatc ggtcgacagc gcgatgttgc gggcgttaat
     4501 gccggcgcgg acctcgccga tcttctgcaa cgaccagatg tcgtattttt ccacccagcc
     4561 atcgcgcgac atgaagtaga cgaagcggcc atcgggcgag aattttggtc cgccatgcag
     4621 ggcaaagcgg gtcgggaacc gggtcagcgg ttcgagcttg tcgccatcga ggatggtcgc
     4681 gtggtgatcg ccgatctcga cgacaacgaa caggttgagc ggatcggcac tgaacaccgg
     4741 tttggcaagc gccggcgctg cccgctcgat cgaacggctg gcgctgatct ccatcggccc
     4801 ccaggccggc atttgcggca gcggtctgga gatataggcg gtcagcgcct cgatctcgtc
     4861 cttggccaat gtctggccga agcccggcat ctgcgttgcc tcgcgccctt cggcgatgac
     4921 cttggtgatg gccggcccgc gcaggcgacc catgttctcg ggcagcagcg ccgggccgat
     4981 cgcgccgaga cggccggtgc cgtgacaggc gacacaatgg gtctgataga gctgttcggc
     5041 atcagccgcc gccgccgatg tcgccagcag cggcaggcag acaagcgcca gcaggagttc
     5101 agacagacgc ttcatgactc actccacgga acggggtcac ggtgatgcgg ggggcggcgt
     5161 tgtcgaggcc gatttccgca tcgctgagat agcagccggg gtcctcggcc cagggatcgc
     5221 cggtcacctt caaggcccgg atgcgggtgt tgccgccgca gatcgccaga tgcgcgcagg
     5281 cgccgcagcg accggtgaca gcgcgcggcc gctgcttcag cccggcaaag accggatcgc
     5341 tgagatcggt ccagatgtcg gagaacgcgc gttctttgac attgccgagc gtatggtgcc
     5401 accacatcgt gtcgggatgc acctggccga gattgtctat gttggcgaca ttgacgcccg
     5461 aggcattgcc gccccagcgc acaagcatgc gctccagccg ctcggcctgg tcggggtgat
     5521 gggcgcgcac ccagtgcagc aggtagaccg catcggcgtc gttgttgccg gtgacgattt
     5581 caagatcgcg cccctcgtca atggcgcgga cagcgcgggc gatcatgagg tccatggcgc
     5641 ggcgggtcga ggcccattcg gaatcgcgcc cgcggttctt gttgccgcgc ccggcataga
     5701 ccagatgcga caggtagaat ttcggaaagc gctccgcctc gcacaggtcg atcagcggtt
     5761 cgagatgacg ctcgttctcc tgggtcatgg tgaagcgcac gccgaccttg acgccgcgat
     5821 cgcgcagcag cctgagcgcc tccaccgact ggtcgaaggc gccggccttg cggcgcacca
     5881 catcatgcgt ttccttcagc ccgtcgatgg aaatgccgac ataatcataa tcggcctcgg
     5941 cgatgcggtc ggccatccag gcgtcgatca aggtgccgtt ggttgacagg cagaggaaga
     6001 agcccagcgc tttggcacgt tttgatatct cgaatatgtc gcgtctcagc agcggctcgc
     6061 cgccggagag gatcagcacc ggcacgccga acgccttgag atcgtcgagc gtttcgaaca
     6121 cctgcgcggt cgacagttcg ccgggaaaat cgacatcgcc ggagatcgaa tagcagtgca
     6181 tgcaggtcag gttgcagcgg cggatcaggt tccagatcac cacagggccg gtcatgcgga
     6241 gcggtggagc gttgcccttg ggccccgccg catgggcaga ctgaccgggc ttgaggacag
     6301 ttctgagata atgggagagg cggaacatgg cttttccctc acgcggccgg ttggccggtt
     6361 agtctgagac cggtcttttt caggatacgg ctggaaaaca ggacatcatg cgcccgcgca
     6421 ccggcgcctg cgagcgcggc aatctcgcca actttctgat gcacctcgtc gcgcgatcgg
     6481 ccgtgcacca tggcaaagac attgtagcgc caggccggca ggtggcgcgg ccggcgatag
     6541 caatgcgaga cgaagggcag ggcgccgatg cggggcccaa gttcggcaac gcggctgtcg
     6601 tcgatgtccc agacgctcat gccattggcg atgacaccga gagcgtagtg gttggggatg
     6661 acgccgatgc ggcggatcgc gccgatctcc agcagcgcgc cgagccgttc catgatcact
     6721 gcgcgggatc ggtcgagcgc ccgggcaaga tcgccatagg ggtcgggcgt cagcggaagc
     6781 ccgcgctcgg ttgccttgat gagggcctgg tcggaagctg acagcatcgt cacacctcca
     6841 cccggaaacc gacgaagaat tcctcttcct tcggcatcag ccagactttc aggccggtct
     6901 cttcctcaat ggcgcgggcc gtcgcccgga tccgtgccgg atcgagcgag gagagcacga
     6961 accacatgtt cagcgcgtgg tcccgttcgt agttatgggc gatttcagca tgggcgttga
     7021 ccttggccgt cacctcatcg aaccgctcgg aggggaccga catggcggca agacagaccg
     7081 cgccgccgag ttcctcggag ttccacagcg ggccgaaccg ggagatcgcg ccggcctcga
     7141 ccagcgcgcc gatgcgggcg atcagctcgg tctcgctgag ccccagccgg gcgccggcct
     7201 cggcgaacgg tcgttcgctc accggaaacc caccctgcaa tccgttgatg atgacgcggt
     7261 ctgcatcggt cagagtgatc attccgccgc ctcccttgac cgcgagaaca ccgcgccgcg
     7321 ctgcttgaag cagcgcagcg agaacagcac ggaatgtccg gccagatgcg gcgctgcctg
     7381 ttgcgccagc ccgtcaacca cggccatcgc cgaggcccgg tccctgccgt ggatcatcgt
     7441 gaacaggttg aagcgccagt gcggcagccg gcgcggccgc cggtagcaca gcgtgacgcc
     7501 cggcgcggcg gcaaaaccgt ccgccacctg atcgaccgcc gcctcggcaa cgtcccaaac
     7561 cgccatcgca ttggccgaat agccgaaggc gcgatggcgg acgatgatgc cgaaccgggt
     7621 gatgatgccg cgttcaatca tcgccgcgag acggtcgatg acggcgcttt cagaggtatg
     7681 cagtcgctcg gccacggcag cgaacgggcg cggaaccagc atcagcccct gctcgatggc
     7741 gcccagaagt cgccggtcct cggcgtcggc cttcccgatc agcggcttct gcaccggctt
     7801 ggtcgcgcgg ttgccgtaga gcgagaagcc gagatcgatg tggtaggatt tttccagcgg
     7861 cagattgagc accgccagcc cggtctgttg ctcgattccg gccagcgttg ccgtgacatc
     7921 ggcccagtcg cgcccggtca cgacgaacca cagattatag gcgttttcgc gctcgtaatt
     7981 gtgattgacg ccggcaaacc cgttgatcac cgcggcaacg gcctcgatcc gctctggccg
     8041 agctgccacc gcagccagcg tcgaggcgcc
//
LOCUS       DAOMXG010000110         8062 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_4675_length_8062_cov_9.311602, whole genome shotgun sequence.
ACCESSION   DAOMXG010000110 DAOMXG010000000
VERSION     DAOMXG010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 8062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8062
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4675_length_8062_cov_9.311602"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="PLQ11_08400"
     CDS             <1..737
                     /locus_tag="PLQ11_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HRK24959.1"
                     /translation="PADRLAAKLGLDAAAPLPAASVNDARLADLKRERERIGAVNLRA
                     AEELSEVETTRSDLSREVEELTEAIRRLRKGIESLNSEGRTRLITAFDVVNGHFRNLF
                     TRLFGGGTAELQLIESDDPLEAGLEILAHPPGKKPQVLTLLSGGEQALTATALIFAVF
                     LTNPSPVCVLDEVDAPLDDANVERLCNLLDDMVRETATRFVVITHNPITMARMDRLFG
                     VTMAERGVSQLVSVDLGAAERLAEAG"
     gene            979..1332
                     /locus_tag="PLQ11_08405"
     CDS             979..1332
                     /locus_tag="PLQ11_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HRK24960.1"
                     /translation="MADKITAGEDEALKRRLDQLGKVLDGKRQDAEETARQARKGPDG
                     GLGKAMGTGFRVASELAAAILVGGLIGWQVDAWAGSGPWGLLGFLMLGVLAGFWSVYK
                     LAARPTGGEQPPHNG"
     gene            1370..2134
                     /locus_tag="PLQ11_08410"
     CDS             1370..2134
                     /locus_tag="PLQ11_08410"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HRK24961.1"
                     /translation="MAAGAGPVIDPIHQFEVHPIAKFSLFGLDVSFTNASLYMVIVLA
                     VAALILLVGTAGRSLIPGRLQSMAEMLYEFIEDTCQSTMGSHGRKFLPLVFSLFMFVL
                     LSNLIGLIPGTFTVTSHIIVTFALAAVVILTVLIYGIAKHGLHFFGLFVPSGVPKPLL
                     LLITPIEIISFISRPISLSMRLFANMLAGHIALKVFAGFLLMLGAAGGWFALAPLPLA
                     MIVALTALEFLVAGLQAFVFSVLTCIYLNDALHPGH"
     gene            2185..2412
                     /locus_tag="PLQ11_08415"
     CDS             2185..2412
                     /locus_tag="PLQ11_08415"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HRK24962.1"
                     /translation="MDPNAAKLIGAGLAAIGMGAAAIGVGTIFGNFLSGALRNPSAAD
                     GQFTRAFIGAALAEGLGIFAFVVALILLFVK"
     gene            2452..2994
                     /locus_tag="PLQ11_08420"
     CDS             2452..2994
                     /locus_tag="PLQ11_08420"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HRK24963.1"
                     /translation="MSFSFIGAAYAAGAPTGGGSFPPFDSSTFAGQLFWLAISFGLLY
                     LMVSRVAIPRIGGIISDRRARIENDLNDAAKAQKAAEDAAKAFEANLAQAKAKAQGIA
                     QAAREAASKEADARRHQVEAEQAAKLIAAEKSIAATKAKAMGNVGAIAEEAAAAIVDK
                     LGGKAKAADIAGAVAALAKK"
     gene            3005..3475
                     /locus_tag="PLQ11_08425"
     CDS             3005..3475
                     /locus_tag="PLQ11_08425"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012172783.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. Subunit B is part of the
                     membrane proton channel; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP F0F1 synthase subunit B"
                     /protein_id="HRK24964.1"
                     /translation="MTDTLWVAIGFLIFIGIVVWAGGFKALMSGIDSRGEKIAAELSE
                     AKRLRDEAQALLASYEQKKAEAEKDAATIIEAAKLEAERFKAEAAQKLEDFVARRQKQ
                     AELKISQAESQAAAEVRAAAAELAAQAAGQVLAAQSGTETAFKAALGEIKAKLN"
     gene            3584..4381
                     /locus_tag="PLQ11_08430"
     CDS             3584..4381
                     /locus_tag="PLQ11_08430"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRK24965.1"
                     /translation="MLRRFLPLLFLFPHPANAEQSVTFVSRDATTLNARLFQPSGGDG
                     KRPAIVLMHGCGGLARKDGALFKRHQDWAERFQAAGFVVVLPDSFASRGLASQCSVTN
                     RAINPQKRTGDALGALDFLKARADVDPARIVLMGWSNGGSTVLRVAALAPETPGFARV
                     IAFYPGCRPLLKHEWRAKVPTAILHGKADDWTPIEPCQELARIGGPVTIDVFEGATHG
                     FDAPDTPLRQRKGLAFSKSGDGNATSGTHGPSRQQAINRVMELLRGP"
     gene            complement(4539..6095)
                     /gene="gcvPB"
                     /locus_tag="PLQ11_08435"
     CDS             complement(4539..6095)
                     /gene="gcvPB"
                     /locus_tag="PLQ11_08435"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HRK24966.1"
                     /translation="MLNNQGRPTAAGSAEGAGHPTFTGNRALVQEEALLFEIGRADTT
                     GVDLPPPPKVKVRLGKLRRSGAIGLVGLSEPETMRHYVRLSQKNYGIDTGMFPLGSCT
                     MKHNPRLNEKMARLPGFADIHPLQPQSTVQGALKLMEMLGHWLMTLTAMPAVALSPKA
                     GAHGELAGMMAIKAAIAARGEAATRSVVLVPESAHGTNPATAALIGFSVRAVPADADG
                     TVRVAEVKKLLGPDVAAIMLTNPNTCGLFEPEIAAIADAMHAAGAYFYCDGANFNAIV
                     GKTRPGDLGVDAMHINLHKTFSTPHGGGGPGSGPVVLSERLAPFAPVPFLRRKGSRLE
                     LVEHAKGSKAFGRMTAFHGQMGMYVRAMAYMLSHGADGLRQASEDAVLNANYIRAGLA
                     DLMSQPFGDHPCMHEALFDDQWLKGTGVTTLDLAKAMIDEGYHPMTMYFPLVVHGAML
                     IEPTESESRASLDLFIMAMRDLAMAAKAGHAERFTGAPYHAPRRRLDETRAARQPVLK
                     WTPPKPITAE"
     gene            complement(6095..7435)
                     /gene="gcvPA"
                     /locus_tag="PLQ11_08440"
     CDS             complement(6095..7435)
                     /gene="gcvPA"
                     /locus_tag="PLQ11_08440"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HRK24967.1"
                     /translation="MRYLPHSDADRAAMLAHIGVASVDDLFASIPAAKRLKKLVDLPL
                     ARSEIEVERELARLAAENTSAGSVPSFVGAGAYRHHVPATVDHLIQRSEFLTSYTPYQ
                     PEIAQGTLQVLFEFQTQVAALTGMDVANASMYDGSTGTGEAVLMAHRITRRRKAVLSG
                     GLHRHYADVVRTLSGMAGDEVVTLPADMTAGENLIKALDPTVSCIVVQTPDVFGNLRD
                     LSKLAEACKANGSLLIAVFTEAVSLGLVESPGAMGADIVVGEGQSIGNGLNFGGPYVG
                     LFATRQQYIRQMPGRLCGETVDASGQRGFVLTLSTREQHIRREKATSNICTNSGLCAL
                     AFTIHMTLLGEAGLTRLAELNHAHAVDLAERLEGVPGVEVLTRAFFNEFTVRLPGRAD
                     RIVEKLARRGILAGVPVSRLLPRAGLDDCLIVAATEVTTEADRAGFATKLKEVL"
     gene            complement(7450..7827)
                     /gene="gcvH"
                     /locus_tag="PLQ11_08445"
     CDS             complement(7450..7827)
                     /gene="gcvH"
                     /locus_tag="PLQ11_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HRK24968.1"
                     /translation="MSIVRYSKDHEYIRVDGDIATVGITNYAQEQLGDVVFVDLPAIG
                     KTVAAGAETAVVESVKAASEIYAPVAGTIVEVNSALAATPDMVNADPMGKGWFLKLKI
                     ANKAEVDKLMSEAEYTDYLASIS"
     gene            complement(7867..>8062)
                     /locus_tag="PLQ11_08450"
     CDS             complement(7867..>8062)
                     /locus_tag="PLQ11_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HRK24969.1"
                     /translation="GGRRIGTVTSGGFGPTLNGPLAMGYVEAAFAAPGTEVSLIVRDK
                     PLPARIVSMPFVPNRYVRSK"
BASE COUNT         1390 a         2568 c         2622 g         1482 t
ORIGIN      
        1 cgcctgctga ccggctcgcg gccaaactgg ggctggatgc cgccgcgccg ctgccggcag
       61 cctccgtcaa cgatgcccgg ctcgccgatc tcaagcgcga acgcgagcgt atcggcgcgg
      121 tcaatctcag ggctgccgag gaactcagcg aggtcgagac cacccggtct gatctttccc
      181 gcgaggtcga ggaactgacc gaagcgatcc gccggctgcg caagggcatc gaaagcctca
      241 attccgaagg gcgcacgagg ctgatcaccg ctttcgatgt ggtcaacggc catttccgca
      301 acctgttcac ccgcctgttc ggcggaggaa cagccgaact gcaactgatc gagtccgatg
      361 atccgctcga agccgggctg gaaatactcg cccatccgcc gggcaagaaa ccgcaagtgc
      421 tcaccctctt gtcggggggc gagcaggcat tgaccgccac cgcgctcatt ttcgcggtgt
      481 ttctgaccaa tccctcgccg gtctgcgtgc ttgacgaggt ggatgctccg ctggacgatg
      541 ccaatgtcga gcggctgtgc aaccttctcg atgacatggt gcgcgaaacc gccacgcggt
      601 ttgtcgtcat tacccacaat ccgatcacta tggcgcgcat ggaccggttg ttcggcgtca
      661 ccatggcaga gcgcggcgtc tcccagctgg tttcggtcga tcttggcgct gccgaacgcc
      721 tggccgaggc cggctaggcc tgcggcagcg gcggcaaccg gtccaaaaac cgcggaccat
      781 tgcggcaaca aaggtctaat gcgtcatttt ttctttgttt ttcaacgaat taaacaatcg
      841 ttcccacgct tgacacggtg tcgggccgca catatggtgc gcgcgctttc aaggggttgg
      901 atcagccatc cggtcgcttg ccacttcacg gcttccggtc gcattttttc gggcgctggc
      961 aggcaagggg caacgggcat ggccgataaa atcaccgcag gcgaggacga ggcgctcaaa
     1021 cggcggctcg accagcttgg aaaagtgctt gacgggaagc gtcaggacgc cgaagagacc
     1081 gcgcgtcagg ctcgcaaggg gcccgacggg ggacttggca aggccatggg gaccggcttt
     1141 cgggttgcct cggaactggc cgctgccatc ctggtcggag ggctgatcgg ctggcaggtg
     1201 gatgcatggg ccggcagcgg cccgtggggc ctcctcggat ttctcatgct cggcgtcttg
     1261 gccgggttct ggagtgtata caagctggcg gcacgcccca ccgggggcga acagccgcca
     1321 cacaatggat gacccgcctg cgggcggaac agacgacgag ggctgaatcg tggcagcagg
     1381 tgcagggccg gtcattgatc cgatccatca gttcgaggtt cacccgatcg cgaagttttc
     1441 cctgttcggg ctcgatgtct cgttcacgaa cgcctcgctt tacatggtga tcgtgctggc
     1501 ggtggccgca ttgatcctgc tggtcggcac cgccgggcgt tcgctgatcc ccgggcggct
     1561 gcagtcgatg gccgagatgc tctacgagtt catcgaggat acctgccagt cgacgatggg
     1621 ctcgcatggc cgcaagttcc tgccgctggt gttttcgctg ttcatgttcg tgctgttgtc
     1681 caacctgatc ggcctcattc ccggcacgtt cacggtcacc agccacatca tcgtcacctt
     1741 cgcgctcgcg gcggtggtga tcctgaccgt gctgatctac ggcatcgcca agcatggcct
     1801 gcatttcttc gggctgttcg tgccgtcggg cgtgccgaag ccgctgctgc tgctgatcac
     1861 gccgatcgaa atcatctcgt tcatctcgcg gccgatttcg ctgtcgatgc gactgttcgc
     1921 caacatgctg gccggtcata tcgcgctgaa ggtgttcgcc ggcttcctgc tgatgctggg
     1981 cgccgccggc ggctggttcg cgctggctcc gctgccgctg gcgatgatcg ttgcactgac
     2041 cgcgctcgaa ttcctcgttg ccggcctgca ggccttcgtg ttttcggtgc tgacctgcat
     2101 ctaccttaac gacgcacttc acccgggcca ctaacccttc cttcaaccaa cgactatccc
     2161 tatcccaaat caaggagttc aatcatggat ccgaatgccg ccaagctcat cggcgctggt
     2221 cttgccgcga tcggcatggg cgccgccgcg atcggcgtgg gcaccatctt cggcaacttc
     2281 ctgtcgggcg cgctgcgcaa cccgtcggcg gccgatggcc agttcacccg cgcgttcatc
     2341 ggcgcggcgc ttgccgaagg tcttggcatt ttcgccttcg tcgtcgcgct gatcctgctg
     2401 ttcgtgaagt aatccggctt cgtccggatt acccccacac ggaaaactaa catgtcgttc
     2461 tctttcatcg gcgctgcata tgcggccggt gcgccgaccg gtggcggaag ctttccgccg
     2521 ttcgattcgt ccaccttcgc agggcagctg ttctggctgg cgatcagctt cgggctgctc
     2581 tacctgatgg tctcgcgcgt cgcgatcccg cgcatcggcg gaatcatcag cgaccggcgc
     2641 gcgcgcatcg agaatgacct gaacgatgcc gccaaggcac agaaggccgc cgaggatgcc
     2701 gccaaggcgt tcgaggcgaa ccttgcccag gccaaggcca aggcgcaggg cattgcccag
     2761 gcggcgcgtg aggctgcgtc caaggaagca gatgccaggc gtcatcaggt cgaggccgaa
     2821 caggccgcca agctgattgc ggcggagaag tcgatcgcag caaccaaggc caaggcgatg
     2881 ggcaatgtcg gcgccatcgc cgaagaggcg gcggcggcga ttgtcgataa gctcggtggc
     2941 aaggccaagg cggccgatat tgccggtgcg gttgccgccc tggccaagaa gtgaggagac
     3001 gccgatgacc gataccctct gggttgccat tggcttcctg atcttcatcg ggattgtcgt
     3061 ctgggcaggc ggcttcaagg ccctgatgtc cgggatcgat tcgcgcggcg agaagatcgc
     3121 ggccgaactc agcgaggcca agcggctgcg tgacgaagcg caggccctgc tcgcatccta
     3181 cgagcagaaa aaggccgagg ccgagaagga tgctgcgacc atcatcgagg ccgccaagct
     3241 tgaagccgag cgtttcaagg ccgaggccgc gcagaagctt gaggatttcg tggcccgccg
     3301 gcagaagcag gcggaactga agatttccca ggccgaatcc caggcagcgg cagaggtgag
     3361 ggccgccgcg gcggaacttg ctgcgcaggc cgccgggcag gtgctcgcag cgcagtccgg
     3421 caccgagacg gctttcaagg ccgcgcttgg cgagatcaag gccaagctga actgacattg
     3481 ggcttgacgg attggcgaaa agggccaccg caaggtggct tttttcattg ccgaccggca
     3541 ttcccttcct tgacatcggg acggatccgg tcagccttct ttcatgctgc gccggttcct
     3601 gcccctcctt tttctgttcc ctcatccggc caacgccgag cagtccgtca catttgtctc
     3661 ccgtgatgca acgacgctca atgcgaggct gttccaaccc tctggtggtg acggaaagcg
     3721 gccggcgatc gtcctgatgc acggctgcgg cgggctggcg cgcaaggatg gcgccctgtt
     3781 caagcggcat caggactggg cggaacggtt tcaggctgcc ggttttgtcg ttgttctgcc
     3841 ggacagtttt gcctcacggg ggctggcgtc gcaatgttcg gtgaccaatc gcgcgatcaa
     3901 cccgcagaag cggaccggcg atgccctggg tgcactggat tttctcaagg cccgcgccga
     3961 tgtcgatccg gcgcggattg tcctgatggg ctggtccaac ggcggttcga cagtgctgcg
     4021 ggtcgccgct cttgccccgg aaacgccagg gtttgcccgc gtgattgcct tctatcccgg
     4081 ctgccggccc ctgctcaaac atgaatggcg ggcgaaagtc cccacggcca tcctgcacgg
     4141 aaaggcggat gactggacgc cgattgagcc ttgtcaggaa ttggcgcgca tcggcggccc
     4201 ggtgacaatc gatgttttcg agggggcaac ccacgggttt gatgcgcccg atacgccgct
     4261 gcgtcaaaga aagggccttg ctttctccaa gtcgggcgat ggcaacgcca cctccggcac
     4321 gcatggaccc tcgcgccagc aggccatcaa ccgggtgatg gagctgctgc gcgggccgtg
     4381 acgattttgg attacaccag cagccctcgg agatgacctg tcggtcatcc tcgggcaaga
     4441 ccgtcgcaac gtcttttcaa cagctgctcg gcgtcgcttc gctcctatcc aaccgcctgc
     4501 attctcgacc tgtcggtcat gcatgcaggc cgttggtatc actccgccgt gatcggcttg
     4561 ggtggcgtcc acttcaagac cggctgacgg gcggcgcggg tctcgtcgag acggcggcgc
     4621 ggcgcgtgat acggtgctcc ggtgaagcgt tctgcgtgac cagcctttgc cgccatcgcc
     4681 agatcgcgca tcgccatgat gaacagatcg agcgaggcgc ggctttccga ttcggtcggc
     4741 tcgatgagca tggcgccatg gaccaccagc gggaaataca tggtcatcgg atgatagccc
     4801 tcgtcgatca tcgccttggc cagatcgagc gtggtcacgc cggtcccctt cagccactgg
     4861 tcgtcgaaca gcgcctcgtg catgcaggga tgatcgccga acggctggct catcaggtcg
     4921 gcgaggccgg cgcggatata attggcgttg agcacggcgt cttccgaagc ctgccgcagg
     4981 ccatcggcgc catgcgagag catataggcc atggcgcgca catacatgcc catctggccg
     5041 tgaaacgccg tcatgcggcc aaaagccttg ctgcccttcg catgctcgac cagttccagc
     5101 cggctgccct tgcggcgcag aaacggcacc ggcgcgaacg gtgccagacg ctccgacagc
     5161 accacaggac cggagccggg cccgccgccg ccatgcgggg tcgagaaggt cttgtgcaga
     5221 ttgatgtgca tcgcatcaac gccgagatca cccggccggg tcttgccgac gatggcgttg
     5281 aaattggcgc cgtcgcagta gaaataggcg cccgccgcgt gcatcgcgtc ggcgatggcg
     5341 gcgatctccg gctcgaacag accgcaggtg ttggggttgg tcagcatgat cgccgccaca
     5401 tccgggccga gcagcttctt cacctcggcg acacgcacgg tgccatcggc atcggcgggg
     5461 acggcgcgca ccgaaaagcc gatgagcgcg gcggtggccg gattggtgcc atgggccgat
     5521 tcgggaacca gaacgacgct gcgggttgct gcctcgcccc tggcggcaat cgccgccttg
     5581 atcgccatca tgccggcaag ttcgccatgg gcgccagcct tcggcgacag cgccaccgca
     5641 ggcatcgccg tcagcgtcat cagccagtgg ccaagcatct ccatcagctt cagcgcgccc
     5701 tgcaccgtcg attgcggctg cagcgggtga atgtcggcaa agccgggcag ccgcgccatc
     5761 ttctcgttga ggcgcggatt gtgcttcatc gtgcaggagc ccagcgggaa cattccggtg
     5821 tcgatgccgt agttcttctg gctgaggcgc acataatggc gcatcgtttc cggctccgaa
     5881 agcccgacga ggccgatagc gccggaacgc cggagcttgc ccaggcgcac tttgaccttc
     5941 ggcggcggcg gcagatcgac gccggtggta tccgcccggc cgatctcgaa cagcagcgcc
     6001 tcttcctgca ccagcgcacg gttgccggtg aaggtcggat ggccggctcc ctcggcagaa
     6061 ccggctgcgg tggggcgtcc ctggttgttg agcatcagag cacctctttc agcttggtgg
     6121 cgaagcccgc gcgatccgct tcggtggtca cttcggtggc agcgacgatc aggcagtcgt
     6181 caagcccggc gcgcggcaac aggcgcgaga ccggcacgcc ggcgagaatg ccgcgccggg
     6241 ccagcttctc gacgatgcgg tcggcgcgtc ccggcagacg cacggtgaac tcattgaaga
     6301 aggcgcgggt cagcacctcc acgcccggaa cgccctccag cctttcggcc agatcaacgg
     6361 catgggcatg gttgagttcg gcaagccggg tcaggcccgc ttcccccagc aacgtcatgt
     6421 ggatggtgaa ggcgagcgcg cagagaccgg agttggtgca gatgttcgat gtcgccttct
     6481 cacgacggat gtgctgctcg cgggtcgaca gcgtcagcac aaagccacgc tgccccgagg
     6541 catccaccgt ctcgccgcag agccggcccg gcatctggcg gatgtattgc tggcgggtgg
     6601 cgaacaggcc gacataaggg ccgccgaaat tgaggccgtt gccgatcgac tggccctcgc
     6661 cgacaacgat gtcagcgccc atggcgccgg gcgattcgac cagaccgagc gagaccgcct
     6721 cggtgaacac cgcaatcagc agcgagccgt tggccttgca ggcttcggcc agcttgctga
     6781 gatccctgag gttgccgaac acgtccggtg tctgcaccac gatgcaggac acagtcgggt
     6841 cgagcgcctt gatgaggttt tcgcccgccg tcatgtcggc cggcagcgtc accacctcgt
     6901 cgccagccat gccggagagc gtgcgcacaa catcggcata atgccgatgc agcccgcccg
     6961 agagcaccgc cttgcggcgg cgggtgatgc gatgggccat cagcaccgcc tcgcccgtgc
     7021 cggtcgagcc gtcatacatc gaggcgttgg cgacatccat gccggtcagc gccgcaacct
     7081 gggtctggaa ctcgaacagg acctgcaacg tcccctgcgc gatttccggc tgatagggtg
     7141 tgtaactggt caggaattcg gaacgctgga tcaggtgatc caccgtcgcc gggacgtgat
     7201 ggcgataggc gccggcaccg acgaaggacg gcaccgagcc ggcagaggtg ttctctgccg
     7261 caagccgcgc aagctcgcgc tcgacctcga tctcggatcg ggcgagcggc agatcgacca
     7321 gcttcttcag ccgttttgcg gcgggaatgg atgcgaacag atcatcgacg gaggcaacgc
     7381 cgatgtgcgc cagcatcgcg gcacgatcgg catccgaatg gggcaggtag cgcatggacg
     7441 ggtcccggct cagctgatcg aggcaaggta atcggtgtat tcggcctcgc tcatcagctt
     7501 gtcgacctcc gccttgttgg cgatcttcaa cttgagaaac cagcctttgc ccatcggatc
     7561 ggcattgacc atgtcagggg tcgcggcaag cgcgctattg acttcaacga tggtgcccgc
     7621 gaccggcgcg tagatctcgc tggccgcctt gacgctctcg accaccgccg tctcggcgcc
     7681 cgccgccacc gtcttgccga tggcgggcag atcgacgaac accacgtcgc cgagctgttc
     7741 ctgcgcgtag ttggtgatgc cgaccgtggc gatatcgcca tcgacgcgga tgtattcgtg
     7801 atccttgctg taacggacga tgctcatggg tcggaacctt ccggaaaggg gttgcttgga
     7861 caggagttac ttggaacgaa cgtagcgatt gggaacgaac ggcatcgata cgatgcgcgc
     7921 cggcaggggc ttgtcgcgga cgatcaggct aacctcggtg ccgggggcag cgaacgccgc
     7981 ctcgacatag cccatcgcca gcgggccatt cagcgtcggg ccgaagccgc cggaggtgac
     8041 tgtgccgatg cgccggccgc ca
//
LOCUS       DAOMXG010000111         8047 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_4688_length_8047_cov_9.373373, whole genome shotgun sequence.
ACCESSION   DAOMXG010000111 DAOMXG010000000
VERSION     DAOMXG010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 8047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8047
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4688_length_8047_cov_9.373373"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="PLQ11_08455"
     CDS             <1..682
                     /locus_tag="PLQ11_08455"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HRK24970.1"
                     /translation="EMAAIADDRAVRAVVIAAEGPAFSAGHDLKEMTARRADADRGRA
                     YFTDIMGRCATMMKAVVRSPKPVIAAVEGVATAAGCQLVASCDLAVAGMNAKFCTPGV
                     HIGLFCSTPMVALTRNLAPKHALEMLLLGEMVPAAEAYRVGLINRVVPAGKALDAARE
                     MAAIIASKSPLTLKIGKEAFHRQREMDLDAAYAYAAGVMVDNMLARDAEEGIGAVLGK
                     RRPEWRGE"
     gene            complement(814..1602)
                     /locus_tag="PLQ11_08460"
     CDS             complement(814..1602)
                     /locus_tag="PLQ11_08460"
                     /inference="COORDINATES: protein motif:HMM:NF013397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HRK24971.1"
                     /translation="MPDPIHPTGLDAYKPAEIAQRIEAAGMGKAGLAFVPLATLSVLA
                     GVFIGFGAAAYTMTMTGADLAFGPARFLGGLVFALGLVLVVIGGAELFTGNTLIVMAW
                     VDRKVATRAMLRNWIIAYFGNLAGSLALVALMAVAGMLSGGMGATAAKIATAKVNLGF
                     VEAFTRGILCNALVCLAVWLTFAGHSATDKILAILLPIAAFVLLGFEHCIANMYLIPA
                     GWLAGAPVTAGGFANNLLAVTLGNIVGGAGGVALSYWLAYGRRE"
     gene            1705..2232
                     /locus_tag="PLQ11_08465"
     CDS             1705..2232
                     /locus_tag="PLQ11_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HRK24972.1"
                     /translation="MDHDQYPDAYILDILKTTKTVALVGASDNEARPSFFVLKYLIER
                     GYKVIPVNPGRAGSTMVGQPVFARLADIAEPIDMVEVFRNSEAAAGIVDEALALDPLP
                     KVIWMQLSVRNDAAAAKAEAAGIRVVMNRCPKIEYGRLSGEIGWTGVNSRTISAKRPQ
                     LHGKGAQKLSIWRKV"
     gene            complement(2374..2940)
                     /gene="efp"
                     /locus_tag="PLQ11_08470"
     CDS             complement(2374..2940)
                     /gene="efp"
                     /locus_tag="PLQ11_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003611543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HRK24973.1"
                     /translation="MKIIASNVRKGNILEHEGQLYAVITAESFFPGKGTPTTQIDMRR
                     ISDGVKISLRYKTTEQVERAFVEDKEHTFLYKDGDGYHFMNQENYEQVQVSDDVIGDQ
                     SKFLQENMVCILSMFDGKAVAIELPARVTLEIMETEPVTKGQTASSSYKPAILSNGAR
                     VMVPPHITAGTRVVVQTADGAYVERAKD"
     gene            3134..4414
                     /locus_tag="PLQ11_08475"
     CDS             3134..4414
                     /locus_tag="PLQ11_08475"
                     /EC_number="2.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase"
                     /protein_id="HRK24974.1"
                     /translation="MSEKAPGFNTLAVHAGAAPDAATGARATPIYQTTSFVFDDVDHA
                     ASLFGLQAFGNIYTRITNPTTAVLEERVAALEGGTAALGVASGHAAEHLIFHTLLEPG
                     DEFVAAKKLYGGSINQFNHAYRKFNWNVVWADTDDLASFEKAITPKTKAIFIESIANP
                     GGVVTDIAAIAKIADRAGIPLIVDNTLATPYLIRPFEHGAHIVVHSATKFLGGHGNSI
                     GGVIVDGGKFDWLKYGKKYPSLTQPRPEYSGMVLAETFGNFAFAIACRVLGLRDLGPA
                     LSPFNAFLLLTGIETLPLRMQRHSDNALKVAEHLAKHPKVAWVSYPGLKGDKYHALAK
                     KYSPKGAGAVFTFGLKGGYDAGIKLVSNLKLFSHLANVGDTRSLVIHPASTTHRQLTD
                     EQKVVAGAGPDVVRISVGIEDADDLIADLDQALA"
     gene            4414..4743
                     /locus_tag="PLQ11_08480"
     CDS             4414..4743
                     /locus_tag="PLQ11_08480"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRK24975.1"
                     /translation="MAGPKPRTRFTPAEAAALPFPPGRLSTLAFDDNGLELRHYAPKG
                     RDDQTPHDRDEIYVVISGTGRYRRAGEVVPFGPGDVLYAAAHEEHRFEDFTPDFATWV
                     IFWGETK"
     gene            complement(4786..6282)
                     /locus_tag="PLQ11_08485"
     CDS             complement(4786..6282)
                     /locus_tag="PLQ11_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRK24976.1"
                     /translation="MESFHALMQGFSVALTFHKLMYASLGVLLGTAVGVLPGIGPAVT
                     VALLLPVTFKLDPSGSLIMFAGIYYGAMYGGSTTSILINTPGESASMATSLEGSKMAK
                     SGRAGPALATAAIGSFVAGTLATFGIAFLAPWMVKFAVQFGPEDYFALMVLAFVTVSA
                     TFGNSPLKGMISLFLGLALGLIGIDKLTGQSRLTFGLPELLDGVEVTTLAVGLFAIGE
                     ALYVVSQRQEQGEMMKLSGSLWMSKLDWQRSWKPWIRGTLFGFPIGALPAGGAEIPTF
                     LSYSTEKRLSRHPEEWGTVGAIEGVAGPEAANNASVAGTLVPLLSLGLPTSATAAILL
                     AGFQQFGLNPGPLLFAENAQIVWTLIASLFIANVMLVILNLPLVGLWVQLLKIPTPWL
                     YSGILMFAAMGSIATKGSVAELLMLITFGIMGFLMRRHDYPVAPMVVGLILGPMAESQ
                     LRRALQISLGDPLVLVQSPIAVVILLVAAVAFIAPFVTNTRRFKAEED"
     gene            complement(6293..6772)
                     /locus_tag="PLQ11_08490"
     CDS             complement(6293..6772)
                     /locus_tag="PLQ11_08490"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HRK24977.1"
                     /translation="MNDQQPRVRRDHSAAIVGFFLLFVAGVLFYDTYSVDRAVAYGVG
                     PTAMPKVISGGLAILGVLSLLSAVKGERFEIESTDWRAVAIVVAGFLTLTVMIGSGAG
                     FIPAMTVLFTVTSYAFGRRALMADAAIGVVLATLIYLLFSKLLALSLPQGPIERLFG"
     gene            complement(6869..7801)
                     /locus_tag="PLQ11_08495"
     CDS             complement(6869..7801)
                     /locus_tag="PLQ11_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HRK24978.1"
                     /translation="MAAAMLAGSVVTASAQMELKIMAPAAPGGGWDGTARSMQSALTA
                     AGLAKNIQVTNVPGAGGAIGLAQFVNTAKGDANQLMVNGFVMVGSLITNKSPVNLSMV
                     TPIARLTAEALVIVVPAESKIQNAKELADAMKADISKVTFAGGSAGGVDHILAALIAG
                     TMGLDGSKVNYVPFAGGGEALAALLSNRVTAGISGWGEFEGQIKAGKLRAIAVTSGGK
                     LPGNPTPTLREQGINLELINWRSVVAAPGISADQKKALSDVIDKMVKSKEWQDVLKQR
                     GWDDAYLAEPAFGEFLKAEQTRVEGVLKAVGLVK"
BASE COUNT         1517 a         2642 c         2565 g         1323 t
ORIGIN      
        1 cgaaatggcg gcgatcgccg acgaccgggc cgtgcgcgcc gtggttatcg ccgccgaggg
       61 gccggcgttt tccgccgggc atgatctcaa ggaaatgacc gcccgaagag ccgatgcgga
      121 tcgcggccgc gcctatttca ctgacatcat gggccgctgc gccacgatga tgaaagcggt
      181 tgtccgctcg cccaagccgg tgatcgcggc ggtcgagggg gtggccaccg ccgccggctg
      241 ccagctggtc gccagttgcg accttgccgt tgcagggatg aatgcgaaat tctgcacgcc
      301 gggcgtgcat atcggcctgt tctgctcgac gccgatggta gcgctgaccc gcaatctcgc
      361 ccccaaacat gcgctggaaa tgctgctgct cggcgagatg gtgccggcgg cggaagccta
      421 ccgcgtcggc ctgatcaacc gcgtggtgcc tgccggcaag gcgctggatg cggcgcgcga
      481 gatggcggcg atcatcgcct ccaaatcgcc gctgacgctg aaaatcggca aggaagcctt
      541 ccaccgccag cgcgagatgg atctcgacgc cgcctacgcc tatgcggccg gggtgatggt
      601 cgacaacatg ctggcgcgcg atgccgagga aggaatcggc gcggtgctgg gcaagcgcag
      661 gcccgaatgg cggggggagt agcgcctttt tagtggccct tgccagggca agggcgtccc
      721 tcgcatcagc tcggcgtcgc ttcgctccca accagaaact cccgttgatg accggccggc
      781 catcacgcag agtcgccggg attgaggttg cccttattcc cgccgcccgt aggccagcca
      841 gtagctgagc gcgacaccgc ccgccccgcc gacgatattg ccgagcgtga ccgccaggag
      901 gttgttggca aaaccgcccg ccgtcaccgg tgcgccagcc agccagccgg ccgggatcag
      961 atacatgttg gcgatgcaat gctcgaagcc gagcaggacg aaagcggcga tcggcagcag
     1021 gattgccagg atcttgtcgg tggccgaatg gccggcgaag gtcagccaga ccgcaagaca
     1081 aaccagcgca ttgcagagaa tgccgcgcgt gaacgcctcg acaaagccga gattgacctt
     1141 ggcggtcgcg atcttcgcgg cggtcgcacc catgccgccc gaaagcatcc ccgccaccgc
     1201 catcagagcg acaagcgcca gcgagccggc cagattgccg aaataggcga tgatccagtt
     1261 gcgcagcatg gcgcgcgtcg caaccttgcg gtcaacccag gccatgacga tcagcgtatt
     1321 gccggtgaac agttccgcgc cgccgatcac caccagcacc agcccgagcg cgaacaccag
     1381 cccgcccagg aaccgcgccg ggccaaaggc aagatcggcg ccggtcattg tcatcgtata
     1441 ggctgcggcg ccgaaaccga tgaacacccc ggcaagcacg ctcagggttg cgagcggcac
     1501 aaaggccagg ccggccttgc ccattcccgc cgcctcgatg cgctgggcga tctcggcggg
     1561 cttgtaggca tcaagaccgg tggggtgaat gggatcgggc atcggcgtcc tcgcttgtcc
     1621 ttttgccgat tcatgccggc tccgggcagc cctgtcgaga ggcgaaccct tgacattcgg
     1681 ccgcgcccgc ccaccttgca gcgcatggat cacgatcaat accccgacgc atacattctc
     1741 gacatcctca agaccacgaa aaccgtggcg ctggtgggcg cctccgacaa cgaggcgcgg
     1801 ccgagctttt ttgtgctgaa atacctcatt gagcgcggct acaaggtcat tccggtcaat
     1861 ccgggccgcg ccggctcgac catggtgggc cagccggtgt tcgccaggct cgccgacatt
     1921 gccgagccga tcgacatggt ggaagtgttc cgcaattcgg aagcggcagc gggaatcgtc
     1981 gatgaggcgc tggcgctcga tccgctgccc aaggtcatct ggatgcagct ttcggtgcgc
     2041 aatgacgccg cagccgccaa ggcggaagct gctggcatcc gggtggtgat gaaccgctgc
     2101 ccgaagatcg aatatggccg gctttccggc gagatcggct ggaccggcgt caactcgcgc
     2161 accatttcgg ccaagcgccc gcaactgcac ggcaagggcg cgcagaagct gtcgatctgg
     2221 cggaaagtgt gacgcggacc ctgcgtgccc tttgccttca acgggcgcgg tgaacagccc
     2281 gtgcagtcct ggccaacccc ctcccgcgcc ggtggcgcga cctccccctc aaggggggag
     2341 gaaggctgcc tcagccggcg aggcgtgaac caatcagtcc ttggcgcgtt cgacgtaggc
     2401 gccgtcggcg gtctgaacca cgacgcgggt gccggcggtg atgtgcggcg gcaccatgac
     2461 gcgggcgccg ttcgacagga tcgccggctt gtaggaggac gaggcggtct ggcccttggt
     2521 gaccggctcg gtttccatga tctccagcgt cacacgcgcc ggcagttcga ttgccacggc
     2581 cttgccatcg aacatcgaca ggatgcagac catgttctcc tgcaggaact tcgactggtc
     2641 gccaatgacg tcgtcgctga cctggacctg ttcgtagttt tcctggttca tgaagtgata
     2701 gccgtcgccg tccttgtaga ggaaggtgtg ttccttgtcc tcgacgaagg cgcgctcgac
     2761 ctgctcggtc gtcttgtagc gcagcgagat cttcactccg tcggaaatgc ggcgcatgtc
     2821 gatctgcgtc gtcggcgtgc ccttgccggg aaagaagctc tcggcggtga tgacggcgta
     2881 gagctggccc tcgtgttcga ggatgttgcc cttgcggacg ttgctggcga tgattttcac
     2941 gggctgaccc tgacggttcg attggaaacg gttggcgctc ctttgcccta tcgggaggca
     3001 tttgggaagg ggcgagctgt gacggcccga aagcgccggg caggctgtcg cagggcgcat
     3061 gttctgtttg acgaacaaat cgttcttcga tagcgtttgc caacgttcgg tgcaggatca
     3121 caggagcaag gcgatgagcg agaaggcccc cggtttcaac acactagccg tccatgcggg
     3181 cgccgccccc gatgccgcca ccggcgcgcg ggcgacgccg atctaccaga cgacgtcttt
     3241 cgttttcgat gacgtcgatc acgccgcctc gctgttcggc ctgcaggcgt tcggcaacat
     3301 ctacacccgc atcaccaacc cgacgaccgc cgtgctggaa gagcgcgtcg cggcgctgga
     3361 gggcggcacg gcggcgctgg gcgttgcctc gggccatgcg gccgagcacc tcatcttcca
     3421 cacgctgctg gagccgggcg acgaattcgt cgccgccaag aagctctatg gcggctcgat
     3481 caaccagttc aaccacgcct atcgcaagtt caactggaat gttgtctggg ccgacaccga
     3541 cgatctcgcc tcgttcgaaa aggcgatcac gccgaaaacc aaggcgatct tcatcgagtc
     3601 catcgccaac ccgggcggtg tcgtcaccga catcgcggcg attgccaaga tcgccgaccg
     3661 cgccggcatt ccgctgatcg tcgacaacac gctggcaacg ccctatctga tccgcccgtt
     3721 cgagcatggc gcgcatatcg tcgtgcattc ggcgaccaag ttcctcggcg gtcatggcaa
     3781 ctcgatcggc ggcgtcattg tcgacggtgg caagttcgac tggctgaagt atggcaagaa
     3841 gtatccctcg ctgacccagc cacgcccgga atattccggc atggtgctgg ccgagacttt
     3901 tggcaatttc gcctttgcca tcgcctgccg cgtcctcggc ctgcgcgatc ttggaccggc
     3961 gctctcgccg ttcaacgcct tcctgctgct gaccggcatc gagacgctgc cgctgaggat
     4021 gcagcgccac tcggacaatg cactgaaggt ggccgagcat cttgccaagc atccgaaggt
     4081 cgcctgggtc agctatccgg gcctgaaggg cgacaagtat catgcgctgg ccaagaaata
     4141 ttcgcccaag ggcgccggcg ctgtgttcac cttcggcctc aagggcggct atgacgccgg
     4201 catcaagctg gtgtcgaacc tcaagctgtt ctcgcatctg gccaatgtcg gcgatacgcg
     4261 cagcctggtc atccatccgg cttcaaccac ccatcgccag ctcaccgacg agcagaaggt
     4321 ggtcgccggc gccgggccgg atgtggtgcg catctcggtc ggcatcgagg atgcggacga
     4381 tctgatcgcc gatctcgatc aggcgctggc ctgatggcag ggccaaagcc aaggacacgg
     4441 ttcaccccgg cggaagcggc tgccctgccg ttccctccgg ggcggctttc caccctggca
     4501 ttcgacgaca acgggcttga gctgcgccac tatgcgccga agggccgcga tgaccagacg
     4561 ccgcatgacc gcgacgagat ctatgtcgtg atcagcggga cgggacggta tcgccgggcg
     4621 ggtgaggtgg tgccgttcgg gcccggcgac gtgctctatg ccgccgcgca tgaggaacac
     4681 cgcttcgagg atttcacccc ggattttgcg acctgggtga tcttctgggg cgagacgaag
     4741 taggcattgc gcagggcacg cgagaggccc ggacggcctc tgccttcaat cctcttccgc
     4801 cttgaagcgg cgggtattgg tgacgaacgg cgcgatgaac gctaccgccg cgaccagcag
     4861 gatcaccacc gcgatcgggc tctgcaccag taccagagga tcccccaggc tgatctgcag
     4921 ggcccgcctg agctggcttt ccgccatcgg tccgagaatg aggccgacca ccatcggcgc
     4981 caccggataa tcatgccggc gcatcaggaa acccatgatg ccaaaggtga tcagcatcag
     5041 gagctcggcc accgaaccct tggtggcgat cgagcccatg gcggcgaaca tcaggatgcc
     5101 ggaatagagc cacggcgtcg ggatcttcag aagctgaacc cagagaccga ccagcggcag
     5161 gttcaggatc accagcatga cattggcaat gaacagcgag gcgatcagcg tccagacgat
     5221 ctgcgcattc tcggcaaaca gcagcggacc ggggttgagg ccgaattgct gaaagccggc
     5281 cagcaggatc gccgccgtcg ccgaagtcgg cagccccagt gacagcagcg gcaccagcgt
     5341 tccggcaacc gaggcgttgt tggccgcctc tggtccggcg acaccctcaa tggcgccgac
     5401 ggtgccccat tcctcgggat gcctggacag ccgcttttcg gtcgaatagc tgaggaaggt
     5461 cggaatttcc gccccgcccg ccggcagcgc gccgatggga aaaccgaaca gcgtgccgcg
     5521 gatccacggt ttccaggaac gctgccaatc caacttcgac atccatagcg agccagaaag
     5581 cttcatcatc tcgccctgtt cctggcgctg ggagacgaca tacagcgcct cgccgatggc
     5641 gaacaggccg acggccagcg tcgtgacctc gacaccatcg agcaattcgg gcaggccgaa
     5701 ggtcagccgc gactggccgg tcagcttgtc gatgccgatc aatccaagcg ccagaccaag
     5761 gaagagcgag atcatgccct tgagcgggga attgccgaag gtcgccgaga ccgtgacaaa
     5821 ggccagcacc atcagcgcga aataatcctc agggccaaac tgaacggcaa atttcaccat
     5881 ccacggcgca agaaaggcga tgccgaaggt ggccagcgtg ccggccacga acgatccgat
     5941 ggcggccgtg gccagcgccg gcccggcgcg gccgctcttg gccatcttcg agccttccag
     6001 cgatgtcgcc atcgaggcgc tttcgccggg cgtgttgatc aggatcgagg tggtcgagcc
     6061 gccatacatc gccccgtaat agatgccggc gaacatgatg agcgagccgg acggatcgag
     6121 cttgaacgtc accggcagca gcagcgccac cgtcaccgcc gggccgatgc cgggcagcac
     6181 gccgaccgcg gtgccaagca gcacgccgag gctggcatac atcagcttgt ggaaggtcag
     6241 ggcaacagaa aagccctgca tcagcgcatg gaacgactcc atagccccct cctcaaccga
     6301 acagccgctc gatcgggcct tgcggcagcg acagagccag gagcttggag aacaacagat
     6361 agatcagggt ggccagaacc acgccgatgg ccgcatcggc catcagggca cgccggccaa
     6421 aggcatagga ggtgaccgtg aacagcaccg tcatcgccgg aatgaagccg gcaccgctgc
     6481 cgatcatcac ggtcaacgtc agaaagccgg caaccacgat cgcgaccgcg cgccagtcgg
     6541 tgctttcgat ctcgaaacgc tcgcccttga ctgccgagag cagcgacagg acgcccagaa
     6601 tcgcaaggcc gccggatatc accttcggca ttgccgtggg gccgacgcca taggcaaccg
     6661 cacgatcgac cgaataggtg tcgtagaaca gcaccccggc gacaaacagc aggaaaaagc
     6721 cgacaatcgc ggcggaatga tcgcgccgaa cgcgcggttg ctggtcattc atcgaaagcc
     6781 ccccgattgc cggccagcac tctgctcgcc ggccggcacc atacgcctga cgcgggcagc
     6841 gcccccctgc tgcccgcgct gcactctctt acttgacgag gcccacggcc ttgagaacgc
     6901 cctcgacgcg ggtctgctcg gccttcagga attcgccgaa cgccggctcg gccagatagg
     6961 catcatccca gccgcgctgc ttgagaacgt cctgccattc cttcgacttg accatcttgt
     7021 cgatgacatc cgacagcgcc ttcttctgat cggcgctgat gcccggcgcg gccacgaccg
     7081 agcgccagtt gatcagttct aggttgatgc cctgctcgcg cagcgtcggc gtcgggttgc
     7141 cgggcagctt gccgccggag gtgacggcga tggcgcgcag cttgccggcc ttgatctggc
     7201 cctcgaactc gccccagccg gaaatgccgg cggtcacgcg gttcgacagc agcgccgcca
     7261 gcgcctcgcc gccgccggcg aagggcacgt aattgacctt ggagccgtcg aggcccatcg
     7321 ttccggcgat cagcgcggcc aggatatgat cgacgccacc ggccgagccg ccggcgaagg
     7381 tcaccttgga gatgtcggcc ttcatcgcat ccgccagttc cttggcgttc tggatcttgg
     7441 actcggcagg aaccacgatc accagcgcct cggccgtcag ccgcgcaatc ggggtgacca
     7501 tcgacaggtt gaccggcgac ttgttggtga tcagcgagcc gaccatgacg aagccgttga
     7561 ccatcagctg gttcgcgtcg cccttggcgg tgttgacaaa ctgggcgagg ccgatggcgc
     7621 caccggcgcc gggaacgttg gtgacctgga tgttcttggc cagacccgcc gcagtcagcg
     7681 ccgactgcat ggagcgggcg gtgccgtccc agccgccacc gggcgcggcc ggcgccatga
     7741 tcttcagctc catctgtgcg ctggcggtca cgaccgaacc agccagcatg gccgcagcca
     7801 tggcaccctt gaagaagaca cgcttgttga cagtcatcct ggttttccct cccggaaaga
     7861 atagtcgatt atgcgccaga cactagggaa ccccggcaga cgaggcaagc ctaaagtcct
     7921 agagcacttt ccgaccggat tgaacccatt gcgtttcgcg ggccgcgtag cgatccgggc
     7981 ggaggctggc gaagagcgta tccgtcgata cggccgagcc agcgtccaaa ccgggcgccc
     8041 gcggccc
//
LOCUS       DAOMXG010000112         8036 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_4698_length_8036_cov_8.930209, whole genome shotgun sequence.
ACCESSION   DAOMXG010000112 DAOMXG010000000
VERSION     DAOMXG010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 8036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8036
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4698_length_8036_cov_8.930209"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="PLQ11_08500"
     CDS             <1..826
                     /locus_tag="PLQ11_08500"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRK24979.1"
                     /translation="GPSLAGRGQIATASAVVSISLNVTRFIGPLIAAPLLVLGLEPLA
                     FAVAAVGFATNTYCLSQISRDESAAASVAAGTEEGASVSYATVMRDMLSSAPMRAVLG
                     LQLLTSLLVRPIIDMFPAFADQVFGRAEGGFGMLSAAVGCGAIAGAAYLVGQPAGRAM
                     HRHIVSGSFLFVAMGLLFAYCTNFWAALIVLLIFGLAMTTSGVASTTYVQAQTPMHRL
                     GRVMSIYSVIARLAPAIGALVLGASADQVGLAPAVTVFSLLAFCAVAMAARSLRVA"
     gene            876..1505
                     /gene="eda"
                     /locus_tag="PLQ11_08505"
     CDS             876..1505
                     /gene="eda"
                     /locus_tag="PLQ11_08505"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HRK24980.1"
                     /translation="MMSPRSSILPLLGRNRVIPVLAFNSVAEGLAVIDALADGGVSVA
                     EVTLRTASGLEAIRAIADERPDILVGAGTVLSPRDVEACLKAGARFLVTPGTTPSLRE
                     ALAEVPVPVLPGAATVSEMLTLAELGFAELKFFPAAAAGGLDYLKGVAGPCPHLRFCP
                     TGGIGPGNMADYLALKTVFAVGGSWLTPAAAIAAGDWAAITQLARQATR"
     gene            1502..2251
                     /locus_tag="PLQ11_08510"
     CDS             1502..2251
                     /locus_tag="PLQ11_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HRK24981.1"
                     /translation="MTAEPLTALAGWLAEARCIVGFTGAGISTESGIPDFRSPGSPWL
                     ANRPVAFDQFITSEPARREAWRRKFAMDDLYAGAGPSIGHRFLSRLVADGRSPAIITQ
                     NIDGLHQAAGTPPDKVIELHGNGTYAACLDCRRIYELAWVRARLEAEATSPRCLACDG
                     LVKSATISFGQPMPEAAMRRAQELATTCDLFLVLGSSLVVYPAASLPLIARRAGARLV
                     IINREPTELDRAADLVIHADIGPTLSRMGNY"
     gene            2361..2981
                     /locus_tag="PLQ11_08515"
     CDS             2361..2981
                     /locus_tag="PLQ11_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HRK24982.1"
                     /translation="MASDVFGDKKIPVMPAAQGQSAELLRQDGTLDQNLDLIQISGVI
                     KWFDVAKGYGFIIPDNGLPDILLHVTCLRRDGFQTAQEGARIVCEVVQRARGYQAFRV
                     LSLDPSTAAHPSTLPAARTHVTVTAEGGYERAMVKWFNRLRGFGFLTRGDGTPDIFIH
                     METLRRFGIAELRPGQWVLVRFGAGPKGQMAADIRLEDGPSGPMSH"
     gene            3055..3588
                     /locus_tag="PLQ11_08520"
     CDS             3055..3588
                     /locus_tag="PLQ11_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017486264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HRK24983.1"
                     /translation="MTSSSEMPVPNRRWFVVAFVAVTLLVSGISLWTNLSRMGEGASA
                     VAKAPEGEQGKLEILTMTGKYPFKVDVMRTDADRAKGLMFRQSMPGEYGMLFDFERDQ
                     QVMMWMKNTYISLDMIFIRADGTVHRIERRTEPHSERTISSGIPVRAVLEVNAGISDK
                     LGLKPGDRIFHPMFPAK"
     gene            complement(3602..4159)
                     /locus_tag="PLQ11_08525"
     CDS             complement(3602..4159)
                     /locus_tag="PLQ11_08525"
                     /inference="COORDINATES: protein motif:HMM:NF021602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2335 domain-containing protein"
                     /protein_id="HRK24984.1"
                     /translation="MSDDPSKSMRRPTDNAAGNSTTPDRPDSADGKVTEAIARVLKDA
                     GTRPTDAPRVAQQVIMTLESFHSGPLPPARDFAAYEQICPGAARDILNMAKEEQRQRY
                     EIERKDLNGAIFLNLVGMLCAVSVIGGMIFAAVYLAKNDKTAAAVAIVTGTSVASVIA
                     AFVRKSNKNPAELPQKQPPKKNKKN"
     gene            4541..4617
                     /locus_tag="PLQ11_08530"
     tRNA            4541..4617
                     /locus_tag="PLQ11_08530"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4575..4577,aa:Pro,seq:tgg)
     gene            4654..4959
                     /locus_tag="PLQ11_08535"
     CDS             4654..4959
                     /locus_tag="PLQ11_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ETC complex I subunit"
                     /protein_id="HRK24985.1"
                     /translation="MTARIYKPARNAMQSGQAKAKRWMLDFEPEARREVEPLMGWTSS
                     SDMQAQVRMWFDSKEAAIAYAERNGIPYRVEEPRERVPRSASYSDNFKFNRVGQWTH"
     gene            4979..5055
                     /locus_tag="PLQ11_08540"
     tRNA            4979..5055
                     /locus_tag="PLQ11_08540"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5013..5015,aa:Arg,seq:tct)
     gene            5117..6793
                     /locus_tag="PLQ11_08545"
     CDS             5117..6793
                     /locus_tag="PLQ11_08545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019904035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRK24986.1"
                     /translation="MMDRLIDTLSRSHARALAVLFGLTLLFFVPGQFTLQPMDRDEPR
                     FAQASKQMMETGDYVDIRFQEEARHKKPVGIYWMQVAAVKLAEAAGAKDARGTIGYYR
                     IPSLIGAMLVVGLTYWTALAFLARPAALMAAALMAGSILLGVEARLAKTDAVLAATVV
                     AAMGALARSYFAFVSPDVRGAPGLGTALVFWIAIGIGILVKGPITPLIPLIVALFLAW
                     RERSFAWLKPMRPAMGLAIVLAIALPWLVAIAIKSGGSFFAESVGKDMLGKVGSVAEK
                     HWGPPGSYFFAFWATFWPGAPLVALTGWFWWSDRKDDAVTFLLAWILPMWLVLEAMPT
                     KLPHYVLPLFPAVAILIMMAVERRAIPFDGWRLPVSAGLMLLIPAALLIGAPAAFWWF
                     DRALPLAAMPLIGLATVLAAVAARSLWQGEVRGALLVGLVAAVPLSIAAFRFGVPELK
                     AINLSRHLAEVARSAGCDRPAFATAGFREPSLVFLTATDLDMMSGGEVPAFLYAPEGG
                     CRIAFVESREEAEFNRLLPPMATGHSLLGRVKGININGGRTLDIGVWIKR"
     gene            6840..7580
                     /locus_tag="PLQ11_08550"
     CDS             6840..7580
                     /locus_tag="PLQ11_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRK24987.1"
                     /translation="MNQSSAPRPVISVVVPVRNEEGNIAPLVAEISAAVFSLGPYEVI
                     YVNDGSTDGTGRVLDELKASRPWLRHIVHDKSTGQSAAVRSGVRHARAEIVCTLDGDG
                     QNDPAFIPVLFGALHAAGPAAGLAQGQRVGRQDTAFKKLQSRIANTVRGAILKDGTRD
                     SGCGLKCFRRDAYLSLPYFDALHRFMPALMVREGFKVVHADVVDRPRGSGTSNYRFFD
                     RFWVGITDLAGVWWLIRRKKPTPLAQER"
     gene            7590..7919
                     /locus_tag="PLQ11_08555"
     CDS             7590..7919
                     /locus_tag="PLQ11_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HRK24988.1"
                     /translation="MFVMQLSKDIGGYLYDVFIEKADLWLLIGIGGQLLFTARFLVQW
                     IASERAGRSVVPLAFWLFSIGGGLITLVYGIKRREPVIILGQSLSLFIYARNLMLIAR
                     ERREKAE"
BASE COUNT         1234 a         2448 c         2669 g         1685 t
ORIGIN      
        1 cggcccgtcg ctggccgggc gcgggcagat cgccacggcc tcggcggttg tttcgatttc
       61 gctcaatgtc acccgcttca tcgggccgct gatcgccgcc cccctgctgg tcctggggct
      121 tgaaccgctg gcctttgccg tggcggcggt gggttttgcc accaatacct attgcctgtc
      181 gcagatcagc cgtgatgaaa gcgccgccgc ttcggtggcg gccggcacgg aggagggcgc
      241 gtcggtcagc tacgcaaccg tgatgcgcga catgctgtcg agcgccccga tgcgggccgt
      301 gcttggcctg caactgctga catcgctgct ggtgcggccg atcatcgaca tgttcccggc
      361 ctttgccgat caggtgttcg gccgtgccga aggcgggttt ggcatgctga gcgctgccgt
      421 cggttgcggt gccatcgccg gggcggcgta tctggtcggc cagccggccg ggcgggccat
      481 gcaccggcat atcgtttccg gctcgtttct gttcgtggcg atgggcctgc tgtttgccta
      541 ttgcaccaat ttctgggctg cgctgatcgt gctcctgatc ttcggcctgg cgatgacgac
      601 cagcggcgtt gcctcgacga cctatgtgca ggcgcagacg ccgatgcacc ggctgggccg
      661 ggtgatgagc atctattcgg tcatcgcgcg gctggccccg gcgatcggag cgctggtgct
      721 cggcgccagt gccgatcagg tgggtctggc gccggccgtc accgttttct cgctgctggc
      781 gttctgtgcg gtggcgatgg cagcacggtc gttgcgcgtg gcgtgaggat tatgcaggat
      841 tgggacgcca gatgcgcccc ccccccggag agccaatgat gtcccctcga tcctcgatcc
      901 tgccgctgct ggggcgcaac cgggtcattc ccgtgctggc attcaacagc gtcgccgagg
      961 gactggcggt gatcgatgcg ctggccgatg gtggcgtgag cgtggccgag gtgaccttgc
     1021 gcaccgcctc gggactggaa gcgatccgcg cgattgcgga cgagcggcct gacattctgg
     1081 tcggggcagg gacggtgctg tccccgcgcg atgtcgaggc ctgtctgaag gccggagcgc
     1141 ggtttctggt cacccccggc acgacaccgt ccctgcgcga ggcgctggcc gaggttccgg
     1201 tgccggttct gccgggcgcg gcgacggttt cggaaatgct gacactggcc gaactcggct
     1261 ttgccgaatt gaagttcttc ccggctgccg ccgccggcgg cctcgactat ctcaaggggg
     1321 tggccggccc ctgtccgcat cttcgcttct gccccaccgg gggcatcggg cctggcaaca
     1381 tggcggatta tctcgccctc aagacggtct ttgcggttgg cggctcgtgg ctgacgccgg
     1441 ccgcagccat cgcggcaggg gactgggcgg cgatcacgca gctggcccgg caggcgaccc
     1501 ggtgacagcc gagccgctga ccgcgctggc tggctggctg gccgaggcgc gctgcatcgt
     1561 cggctttacc ggcgcgggta tttcgaccga aagcggcatt cctgatttcc gctcgcccgg
     1621 cagcccgtgg ctggccaacc gtccggttgc ctttgaccag ttcatcacct ccgaaccggc
     1681 gcggcgcgag gcctggcggc gcaagtttgc catggacgat ctctatgccg gggccggccc
     1741 gtcgatcggc catcggtttc tctcccggct ggtggccgac ggacgctcgc cggcgatcat
     1801 cacccagaat atcgacgggc tgcatcaggc cgccggcacg ccgccggaca aggtgatcga
     1861 actgcatggc aacggcacct atgccgcctg ccttgactgc cgcaggatct acgaactcgc
     1921 ctgggtgcgg gcgcggctgg aggcggaagc gacaagtccg cgctgtctgg cctgtgacgg
     1981 attggtcaag tcggcgacga tctccttcgg ccagcccatg ccggaagcgg cgatgcgccg
     2041 cgcgcaggag ctggcgacga cctgcgatct gttcctggtg ctgggctcct cactggtggt
     2101 ttatccggcc gcaagcctgc cgctgatcgc ccgccgcgcc ggcgcccgtc tggtcatcat
     2161 caaccgcgaa ccgacagaac tcgaccgcgc agccgatctg gtgatccacg ccgacattgg
     2221 tccaaccctg tcccggatgg ggaactattg attggaaaac tttgtcggaa ccaccaatac
     2281 ctgtttgcag acttcatcgc tttgctattc tgttcaacag attcgcgcgt tttgcgggcg
     2341 cgcttgggtg ggttcagacg atggccagtg acgtttttgg cgacaagaag attccggtga
     2401 tgccggcggc gcagggccag tctgcggagc tgttgcggca ggacggaacg ctcgatcaga
     2461 atcttgatct cattcagatt tctggcgtga tcaaatggtt tgacgtggcc aagggctatg
     2521 gctttatcat tcctgacaac ggcctgcccg acattctgct gcatgtgacc tgcctgcgtc
     2581 gcgatgggtt ccagacagcg caggaaggcg ccaggattgt ctgcgaggtt gtccagcgcg
     2641 cccgcggtta tcaggccttc cgcgtgcttt cgctggatcc gtccaccgcc gcccatccgt
     2701 ccacgctgcc ggcggcgcgc acccatgtga cggtgacggc cgagggtggc tacgagcggg
     2761 cgatggtgaa gtggttcaac cgtctgcgcg gcttcggctt cctgacccgc ggtgacggca
     2821 cgccggacat tttcatccat atggagaccc tgcgccggtt cggtattgcc gaactgaggc
     2881 cggggcaatg ggtgctggtg cggttcggcg ccggtcccaa ggggcagatg gcggccgata
     2941 tccgactgga ggatggaccc tccgggccga tgtcgcactg atcggcagca gctggcctct
     3001 tgaggtgcag gttgtggcgg cgtatgtctg tcggcccgaa cgacaggtgt gccgatgacg
     3061 tcgtcctctg aaatgccggt gccgaatcgc cgctggtttg ttgttgcctt cgtggctgtg
     3121 acgctgctgg tcagcggcat ctcgctgtgg accaatctca gccgcatggg cgagggcgct
     3181 tcggccgttg ccaaggctcc ggagggcgag cagggcaagc tcgagatcct caccatgacc
     3241 ggcaagtatc cgttcaaggt cgatgtgatg cgcaccgatg cggaccgtgc caaggggctg
     3301 atgttccgcc agtcgatgcc gggcgaatac ggcatgctgt tcgatttcga gcgcgatcag
     3361 caggtcatga tgtggatgaa gaacacctat atttcgctcg acatgatctt catcagggcc
     3421 gatggcacgg tgcaccggat cgagcgccgc accgagccgc attccgagcg cacgatcagt
     3481 tcgggcattc cggtgcgggc ggtgctggag gtcaatgcgg gaatttccga caagctgggc
     3541 ctgaagccgg gcgatcgcat ttttcacccg atgttcccgg ccaagtgatg aaatcgtata
     3601 ttcagttttt cttatttttc ttaggtggtt gcttctgcgg caattcagca gggttcttat
     3661 tcgactttcg tacaaatgcc gcaataacag atgcaacgct tgtcccagtt actatcgcaa
     3721 cggcggcggc agttttatcg ttcttcgcca gatagaccgc agcaaaaatc atgccgccaa
     3781 tgacggaaac agcgcagagc atcccaacaa gatttaaaaa aattgctcca ttcagatctt
     3841 tacgctcaat ctcatatcgc tggcgctgtt cttcctttgc catattaagt atgtcgcggg
     3901 cagcgcctgg gcatatctgc tcatatgctg cgaaatcgcg cgctggcgga agtggtcctg
     3961 agtggaaaga ttccaaggtc ataatgacct gttgagccac tcgtggagcg tctgtcggtc
     4021 ttgtcccggc gtccttcagg acgcgagcaa tcgcttccgt cactttcccg tcggcggagt
     4081 ctggccgatc aggcgttgtc gagttccctg cggcgttgtc tgtaggacgc cgcattgact
     4141 ttgatggatc gtccgacatc gcgccaaacc tctgtaacag aataggtccc ggcccgctgt
     4201 cctgttacct cgaaagctcc cgacaccaaa agcgcaggtg cagccaagcc agtggtaaat
     4261 cccgcgaaga aggcctttga gcggcgtcgg ctggtcatct tttttgacat tgctcccttc
     4321 tcctgtgtag cggttcacaa aggttggtca agtcaacgat ggccacattc actttagaat
     4381 tcgttcccat tatgacaacg attagcttgt ttcaatcgtt tttgaggcgc gtgacaaatt
     4441 tcaggtgcga gttgcgacgg attgccttta gcgaagtggt tcgggcttgt ggtctcagcc
     4501 tgaggcatga tagccgatcc tctggatgag cggcgtgatc cggggtatag cgcagcctgg
     4561 tagcgcatct gctttgggag cagagggtcg caggttcaaa tcctgctgcc ccgaccagcc
     4621 gccgtccgcc attgaagagc cgtgagggat ccgatgaccg cccgcatcta caagcccgcc
     4681 cgcaacgcga tgcagtccgg ccaggccaag gccaagcgct ggatgctgga tttcgagccc
     4741 gaggctcgcc gcgaggtcga gccgctgatg ggctggacct cctcgtccga catgcaggcg
     4801 caggtgcgca tgtggttcga cagcaaggag gcggccatcg cctatgccga gagaaacggc
     4861 attccctacc gcgtcgagga accgcgcgag cgggtgccgc gctcggcttc ctattcggac
     4921 aatttcaagt tcaaccgcgt cggccagtgg acccactgac cgttcggccc tccgaaccgg
     4981 ccccttagct cagctggata gagcacgtgc cttctaagca tgaggtcgca ggttcgaacc
     5041 ctgcaggggt cgccatggcg cccggagcag ggcggtatgg ctcgcttttg ccgcctgcct
     5101 gtgccacaga ggcggcatga tggaccgtct gattgacacc ctgtcgcgtt cgcatgcccg
     5161 cgcgcttgcc gtgctgttcg ggctgacgct gctgttcttc gtgcccggcc agttcacgct
     5221 ccagccgatg gatcgcgacg agccacgctt tgcccaggcc tccaaacaga tgatggagac
     5281 cggcgattac gtcgatatcc gttttcagga agaggcccgc cacaagaagc cggtcggcat
     5341 ctactggatg caggtcgctg cggtgaagct ggccgaggcg gccggggcga aggatgcgcg
     5401 cggcaccatc ggctattacc gcatcccctc gctgatcggc gccatgctgg tggtcgggtt
     5461 gacctactgg acggcgctgg cgtttctggc ccggcccgcg gcgctgatgg ctgcggcact
     5521 gatggcgggg tcgattcttc tgggggtcga ggccaggctt gccaagaccg atgccgttct
     5581 ggcggcgacg gttgtggcgg cgatgggtgc gctggcgcgt tcttacttcg ccttcgtctc
     5641 gcccgatgtg cggggagcgc ccggcctcgg cacggcgctg gtcttctgga tcgccatcgg
     5701 cattggaatt ttggtgaaag ggccgatcac gccgcttatt ccactgatcg ttgcgctgtt
     5761 tctggcctgg cgggagcgtt cctttgcctg gctgaagccg atgcggccgg cgatggggct
     5821 ggcgatcgtg ctcgccatcg cgttgccatg gctggtggcg atcgccatca agtcgggcgg
     5881 ctcgttcttt gccgaatcgg tcggcaagga catgctcggc aaggtcggaa gcgtggccga
     5941 aaagcactgg ggaccgcccg gttcctattt cttcgccttc tgggcgacct tctggcccgg
     6001 cgctccgttg gtggcgctga ccggctggtt ctggtggtcg gatcgcaagg acgatgcggt
     6061 gaccttcctg ctcgcctgga tcctgccgat gtggctggtt cttgaagcga tgccgaccaa
     6121 gctgccgcat tacgtgctgc cgttgtttcc ggcggtggcg atcctgatca tgatggcggt
     6181 ggagcggcgg gcgattccct tcgatggctg gcgcctgccg gtctcggcgg ggttgatgct
     6241 gctgatcccg gctgcactgc tgatcggcgc tccggcggcc ttctggtggt ttgaccgggc
     6301 gttgccgctg gcggcgatgc cgctcatcgg gctggcgacg gtgcttgcgg ccgtggcggc
     6361 gcgcagcctg tggcagggcg aggtgcgcgg tgcgctgctg gtggggcttg tcgcggctgt
     6421 gcccctgtcg attgctgcct tccgctttgg cgtgccggag ctgaaggcga tcaatctgag
     6481 ccggcatctg gccgaggtcg cccgctctgc cggatgcgac aggccggctt ttgccaccgc
     6541 aggctttcgc gagccgagcc tggtgtttct caccgcgacc gatctcgaca tgatgagcgg
     6601 cggcgaggtt ccggcctttc tctatgcgcc ggaaggcggc tgccgcatcg ccttcgtcga
     6661 atcgcgcgag gaggcggagt tcaaccggtt gctgccgccg atggccaccg ggcattctct
     6721 gctcggccgc gtcaagggca tcaatatcaa tggcggccgc actcttgaca taggggtatg
     6781 gatcaagcgc tgacaggcgc tgccgattgg tctacaagcg cgccggattg gtggaacaga
     6841 tgaaccagag cagcgccccg cgtcccgtga tttccgtggt cgttccggtc aggaacgaag
     6901 agggcaatat cgcgccgctg gtggccgaaa tcagcgcggc ggtgttttcc ctcggcccct
     6961 acgaggtcat ctacgtcaat gatggctcga ccgacggcac cggccgtgtg ctcgacgaat
     7021 tgaaggcgag ccgtccctgg ctgcgccata tcgtccacga caaatcgacc ggccagtcgg
     7081 cggcggtgcg gtccggcgtc cgccatgccc gggccgagat tgtctgcacg ctcgatggcg
     7141 acggccagaa tgatccggcc ttcatccccg tgctgtttgg cgcgctgcat gcggcaggcc
     7201 ccgccgccgg gctggcgcag ggccagcggg tcggccggca ggataccgcg ttcaagaagc
     7261 tgcaatcgcg cattgccaac acggtgcgcg gcgcgatcct caaggatggc acgcgcgatt
     7321 ccggctgcgg tctcaagtgc ttccgccgag atgcctatct gagcctgccg tatttcgatg
     7381 ccttgcatcg cttcatgccg gcgttgatgg tgcgcgaggg gttcaaggtc gtccatgccg
     7441 atgtagtcga ccggccgcgc ggctcgggca cctccaacta ccggttcttc gaccggttct
     7501 gggtcggcat caccgatctg gcgggtgtct ggtggctgat ccgccgcaag aagccgacgc
     7561 ccctggcgca ggaaaggtag gcgtcgatca tgttcgtcat gcaattgtcg aaggacatcg
     7621 gcggttatct ctacgacgtc tttatcgaaa aggccgatct gtggctgctg atcggcatcg
     7681 gcgggcaatt gctgttcacc gcgcgcttcc tggtgcagtg gattgcatcg gaacgggcgg
     7741 ggcggtccgt tgtgccgctg gccttctggc tgttttcgat cggaggcggg ctgatcacgc
     7801 tggtctacgg catcaagcgc cgcgagccgg tcatcatcct cggccagtcg ctgtcgctgt
     7861 tcatctatgc ccgcaacctg atgctgatcg ccagggagcg gcgcgaaaag gcggaatgac
     7921 ggcgggcgat ctgttctttc aaggacgccc cgtgtcagct gcccgccttc tttgacgcca
     7981 acagagcatg tcgcgattaa gtggataccg gttaatcgca gaagacatgc tctaac
//
LOCUS       DAOMXG010000113         8004 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_4728_length_8004_cov_8.423701, whole genome shotgun sequence.
ACCESSION   DAOMXG010000113 DAOMXG010000000
VERSION     DAOMXG010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 8004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8004
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4728_length_8004_cov_8.423701"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2359)
                     /locus_tag="PLQ11_08560"
     CDS             complement(<1..2359)
                     /locus_tag="PLQ11_08560"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HRK24989.1"
                     /translation="MQDLIRDFVIETIEGLEAIDADLVQFERDPANSVLLGRMFHLLH
                     SIKGACGFLGLGRLSTLAHAAEDLVEGFRAGHKPQPDDVTLVLATLDQIRVLVAVLGE
                     TGCEPEGNDAGLIRQIAQATRSAAEAGAPVTFGGLPSDAESRGKDSGERPAYPDDVPL
                     MRQKVRVAVDRLDHLMSMVSELVLVRNQLIDLSRRDDAGGYKLPLQRLSHITGALQDG
                     VMQTRMQSVGGVFARMSRLVRDMSRDLGKEIRLEFGGADTEIDRQMLDVLKDCLGHLI
                     RNSAAHGIEEPSERRAAGKPAHGIIRLMAGHEGSSIVCEVRDDGRGLNLQAIGARALA
                     LGLVSADALGQMNEAEVARLILAPGFSTARAVTQLAGRGVGMDAVREALDKIGGFIEL
                     DNRPGQGLGIRLKLPLTLAMAGVLIIETAGQRYALPQAAVVELVRSNPEGDVRIEMLA
                     SQPTLRMRDRLIPVLDLAGILGFEATVTDSGLIVVAEFGGRMFGLSVEAIHETEEIVV
                     KPMPSRLRQSPFFSGATILGDGRVVLILEPAGFAALLGPAAADAAEQPGAAEAEAGPA
                     SLPLLLVRAGAGVTRVVPLALVSRIEEVPLSSIETLGSRRVVGYRGGLLPVVELGEAM
                     NRSADSLVPMLVFRHAGRSVGVLVDSIVDVVDAPLRVDAEVDVPGCCGSAILAGETVL
                     VLDVPGLFQAGGTELAGDGERRVLLAEASEFCRAMLQPVISGAASCLEVARSAEEARA
                     TLARQRFDVVVIGVDTAEMLDLLEAAAGEAGLGSRFIALTARPGGR"
     gene            complement(2669..3328)
                     /locus_tag="PLQ11_08565"
     CDS             complement(2669..3328)
                     /locus_tag="PLQ11_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphotransferase family protein"
                     /protein_id="HRK24990.1"
                     /translation="MSEQGKSITLDSMDLAALLCSRVCHDVISPVGAIVNGLEVLDDD
                     NDPATQEFALSLIRKSAQQASARLQFCRLAFGAAGSAGAAIDLGDAEQVSRNFFQDDK
                     TQLVWEAPRLLLAKNKVKLLLNLLVIAAATIVRGGTLTVRLTDAADVTNMTVEAKGAM
                     VRMQPHTAELVAGRSESGGVDAHAIQGYYTGLVARAAGMAVAFDLAEDRLTITAGPAA
                     A"
     gene            3550..4164
                     /locus_tag="PLQ11_08570"
     CDS             3550..4164
                     /locus_tag="PLQ11_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1134 domain-containing protein"
                     /protein_id="HRK24991.1"
                     /translation="MRLCTLTRRQGTAWFALWSLLVAVVALSGPALAQSRSRAPANSG
                     ETYGTSELVGSGHRFFGNVSRGLALTIEEAVRRFGEPNGYVLGQEASGAFVGGLRFGE
                     GTLYTRNAGDRKVFWQGPSIGFDFGGDGDRTMMLVYNLPRVSEMFRRFVGLDGSAYFI
                     GGFGMTALASDNIVVVPIRSGVGARLGVNIGYLKFTDNPTWNPF"
     gene            4281..5477
                     /locus_tag="PLQ11_08575"
     CDS             4281..5477
                     /locus_tag="PLQ11_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK24992.1"
                     /translation="MIEQIMYFLLGLLTAALATIALLPALWRRAVRLTRAELEATLPL
                     SPDEIAAEKDQIRAGYVVELRRLESRHEAAEAAAQALKADLGTRTTAIQIHEATIAEQ
                     SAAILTLRTDLEEARTRIANLELDIDGLEGARQKLSENLAGARAVNQSLSDDFSSLTK
                     RAEEQQIRLASAETDIERLNGRLNEALTRGRQLQEEGHSKSEELRQAARKLREAASQI
                     GIAMRKLEAAEKLAEERQQMIAQLQDERLELIDRGGEYQRERDRERADRHAIQSQLDN
                     LTRQINASTAEAETTRGKQSDLVRELTRSVDNLRNDKRQADEEVSRLRIERTMLESEV
                     LRLRRALQAPQEVTREITRAAQAAPPPAAPPSVAVPTPAPAVKRPPLPATIGSPLPPS
                     EAGERT"
     gene            complement(5488..6243)
                     /locus_tag="PLQ11_08580"
     CDS             complement(5488..6243)
                     /locus_tag="PLQ11_08580"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRK24993.1"
                     /translation="MSKQARVAIITGAGSGVGKASSLALSAAGWAIVLAGRRLKELEA
                     VAEMISSAGGQALAVVTDVTDEASVHALFDSAVRTFGRVDLLFNNAGMSGGGPIEDIS
                     LADWKKVVDTNLTGPFLCTREAVRVMKAQAPRGGRIINNGSISAYAPRPNSAPYTATK
                     HAISGLTRSTALDGRKYDIACGQIDIGNAATEMAMRMASGVPQANGEIAIEPLMDPQC
                     VADAIVTMANMPASANVLFMTVMATKMPFVGRG"
     gene            complement(6264..7424)
                     /locus_tag="PLQ11_08585"
     CDS             complement(6264..7424)
                     /locus_tag="PLQ11_08585"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HRK24994.1"
                     /translation="MRRWLLAAALILHSLTGAGAEPVRIGLVLTMSGPSAALGQHVRD
                     GFHLGLKLGGGRLGPSEAEIELVDDERSAARAGQAAEAMAKAKSPAFIVGPLFSELIA
                     PVLKAATPAGTIVLSPHAGPALLAGKRCQPNFFSLAPQDDQAIEVLTKFAEERNLRRA
                     IIVSTAGEEAEIAATAFRRTFKGEVADRLLIDVAASDFSDALNRIDILKPQALFLHVS
                     GAAGARFIAELHRSGAGSGPVLLGSFGFDQADVAFQGDAVIGAYSGGDWAQGVPGAAS
                     AAFVAAFEAEYGYAPGAAAMRGFDAARLISKALEATSGKTTDRTALADAIRQADIESS
                     RGKFIFGNNNFPIQDLYLTRVEKHTDGKNRVTAVRQVFAQYGDHYAAECPQK"
     gene            complement(7560..>8004)
                     /locus_tag="PLQ11_08590"
     CDS             complement(7560..>8004)
                     /locus_tag="PLQ11_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015822032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HRK24995.1"
                     /translation="VPGLTGRSVAAPVLFDAFARMGGEPEPLSMPRGVLMASNGSLPM
                     PLRHLRKDLPKTASAALSPLLRIAYPPDGARIDLGLGGGPERTDLALKAQGGRPPLIW
                     LVNGQPVVQDELRRQAHWNPDGAGFARVTVMDAAGATDSVTVRLE"
BASE COUNT         1499 a         2730 c         2440 g         1335 t
ORIGIN      
        1 gccggccgcc ggggcgtgcc gtcagtgcga tgaagcggct accgagcccc gcctcccccg
       61 cggcggcttc aagcagatca agcatctcgg ctgtatcgac accgatcacc acaacgtcga
      121 accgctgacg cgcaagcgtt gcacgggcct cttctgccga gcgggcgact tcgagacagg
      181 acgctgcccc gctgatgaca ggttgcagca tcgcacggca gaattcggac gcctcggcga
      241 gcagaacccg tcgttcgccg tctcccgcca gttccgttcc cccggcctgg aacaggccgg
      301 gcacatcgag cacaagaacc gtttcgccgg ccaggatggc gctgccgcag cagcccggaa
      361 catcgacctc ggcatcgacc ctgagcgggg catcgacgac atcgacgatc gaatcgacca
      421 ggacgccgac cgaacggccg gcatgacgga acaccagcat cggcaccagc gaatcggccg
      481 aacggttcat tgcctcgcca agctcgacaa ccggcaggag cccgccgcga tagcccacca
      541 cacgccggct gcccagtgtc tcgatactcg aaagcggcac ctcctcgatc cgggagacaa
      601 gagccagcgg caccacgcgg gtaacgccgg ctccggcacg caccagcagg agcggcaggc
      661 tggccggccc ggcttccgct tccgctgcgc ccggctgttc ggctgcatcc gctgcggcag
      721 gcccgagcaa tgccgcaaat ccggccggtt ccaggatcag gacaacgcgc ccatcgccca
      781 gaatcgtcgc gcccgagaag aacggactct gcctcagccg cgacggcatc ggtttgacca
      841 cgatctcctc ggtctcatgg attgcctcga ccgacaggcc gaacatccgg ccgccgaact
      901 cggcaacaac gatgaggccg ctgtccgtga ccgttgcctc aaacccaagg attccggcca
      961 ggtcaagaac cgggatcagg cgatcgcgca tccgcaacgt cggctggctt gccagcatct
     1021 cgatccgcac gtcgccttca ggattggacc gcacaagttc gaccacggca gcctgcggca
     1081 gggcatagcg ctgaccggcg gtctcgatga tcagcacccc ggccatcgcc agcgtcagcg
     1141 gcagtttcag ccggatcccc agcccctgtc cgggccggtt gtcgagttcg atgaaaccgc
     1201 cgatcttgtc cagggcctcg cgcacagcgt ccattccgac gccgcgccca gccagctgcg
     1261 tcaccgcacg ggcggttgag aagcccggcg ccagaataag ccttgcgact tcggcttcgt
     1321 tcatctgtcc aagggcatcg gcgctgacaa gccccagcgc cagcgcccgc gcgccgatgg
     1381 cctgaaggtt cagcccgcgc ccgtcatcgc gcacctcgca gacgatggaa ctgccctcat
     1441 ggccggccat gaggcgaatg atgccatggg ccggcttgcc ggctgccctg cgttcggacg
     1501 gctcttcaat gccatgggct gcggaattgc ggatcagatg acccaggcaa tccttcagca
     1561 catcaagcat ctgccggtcg atctcggtat cggcgccgcc gaattccagc ctgatctcct
     1621 tgccgagatc gcgcgacata tcccgcacca gccgcgacat tctggcaaaa accccgccca
     1681 ccgactgcat gcgcgtctgc atgacgccgt cctgcagcgc cccggtgatg tgcgacagcc
     1741 tttgcagcgg cagcttgtag ccgcccgcat catcccgccg gctcaagtcg atcaactgat
     1801 tacgcaccag caccagttcg gagaccatcg acatcaggtg atcgagccgg tcgaccgcaa
     1861 cgcgcacctt ctggcgcatc agcggcacat cgtccggata ggccggccgt tcaccgctgt
     1921 cttttccgcg gctctctgca tcagatggta acccgccgaa agtgacaggc gcgccggctt
     1981 ccgcagcgct gcgcgtggcc tgagcgatct ggcggatcag ccccgcatca ttgccctccg
     2041 gttcgcagcc ggtctctccg agaacagcca ccagtacccg gatctgatca agcgtggcca
     2101 gcaccagggt gacatcatcc ggctgaggtt tgtgcccggc gcgaaatccc tcgacgagat
     2161 cctcggcggc atgggctagc gtgctcagac gacccaagcc aagaaagcca caggccccct
     2221 tgatggaatg cagcagatgg aacatccgcc ccagcagcac gctgtttgcc ggatcgcgct
     2281 cgaactgcac gaggtcggca tcgatggcct cgagcccctc gatcgtctcg atgacgaaat
     2341 cgcggatcag atcctgcatg gcgccactgg ttcccgtctc ccgccgcagc gacgggagca
     2401 caccggacga ctatgcctgc caagggttga ggaaaggttt tcgtaacgct atcggccgag
     2461 cgttacgaat tgcccgactt accttgcgaa acaacgcgtt agggcatttt cgagcgaagt
     2521 ggtttccggt tcgcgtcaag aaaatacgaa aacaaaaaga tagagcattt tcaagcgaag
     2581 tggagtccgg ttcgcgtcaa gaaaatgcga aaaacaaaaa agacagagca ctctgacgtg
     2641 agttggagtt cgctgaaagc gctttagatc aagccgccgc agggccggcg gtaatcgtca
     2701 gccgatcctc ggcaagatcg aaggcaacgg ccatgccggc agcgcgcgcc accagtccgg
     2761 tataatagcc ctgaatggca tgggcatcga ccccgcctga ctcggaccgt ccggcgacca
     2821 gctcggccgt atgcggctgc atccgcacca tcgcgccctt ggcctccacc gtcatgttgg
     2881 tgacatcggc cgcatccgtg aggcgcacgg tcagcgttcc gccgcgaacg atcgtcgccg
     2941 ccgcaatgac cagaaggttg agcaacagct tgaccttgtt cttggccagc agcaggcgcg
     3001 gcgcctccca gacgagctgg gtcttgtcgt cctggaagaa attgcggctg acctgctcgg
     3061 catcgcccag atcgatcgcc gccccggcag agccggccgc gccgaacgcg aggcggcaga
     3121 actgcagccg ggcggaagcc tgctgcgcgc tcttgcggat cagcgacagg gcaaattcct
     3181 gcgtcgccgg atcattgtca tcatcgagca cttcaagccc gttgacgatg gcgccgaccg
     3241 gcgaaatcac atcatggcag acgcggctgc acagcagcgc cgccagatcc atggagtcca
     3301 gcgtgatgga tttgccctgt tcgctcatga ccagcccgaa tcactgttgt tggtatcgcc
     3361 acaggggata cacgctcgcg ccggctttga gaatccttca attgcctgaa atgccccggc
     3421 aaccattggt taaccatcgg gaatttcacg gcaaatgtca cgattgcgac ggcctggcgc
     3481 cttgcgatgg cccaacttgg ctgcatgatg ggcgagcagc gaatcgtttg tcgcagggag
     3541 ggaccggcca tgcgcctttg cacgttgaca cgccgacagg gcacagcctg gtttgcatta
     3601 tggtcgctgc tggtcgcggt cgttgcgctg tccggaccgg cgctggcgca gagccggtca
     3661 cgcgccccgg ccaattccgg tgaaacctat ggcaccagcg agctggtcgg ctcgggtcac
     3721 cggttcttcg gcaatgtttc gcgcgggctg gcgctcacca ttgaagaagc cgtgcgccgg
     3781 tttggcgaac cgaacggtta tgtgctcggc caggaagcat ccggggcctt tgtcggtggc
     3841 ctgcgtttcg gagagggcac gctctatacc cgcaatgccg gcgaccggaa agtgttctgg
     3901 caaggcccgt ccatcggctt cgattttggc ggcgatggcg atcgcaccat gatgctggtc
     3961 tacaacctgc cccgcgtcag cgagatgttc cgccgttttg tcggccttga cggatcggcc
     4021 tatttcattg gcggcttcgg catgaccgcg ctcgcctcgg acaacattgt cgtcgtgccg
     4081 atccgcagcg gggttggcgc ccggctgggc gtcaatatcg gctatctgaa attcaccgac
     4141 aatccgacct ggaacccgtt ctgacgcagc agggcggctc ccgggcgacg gccccagcga
     4201 attgacactt ttatcctggc ccgcttcgtg caacaggtgg gctggctccc atagttgctg
     4261 gcgggggccg ggatgcggca atgatcgaac agatcatgta tttcctgctg ggtcttttga
     4321 ccgccgcgct ggccacgatc gcgctgctgc cggcgttgtg gcggcgtgcc gtgcggctga
     4381 cgcgcgctga gctggaagcg acgctgccgc tcagtccgga tgaaatcgcc gccgagaagg
     4441 atcagatccg cgccggctat gtagtcgaac tgcgccggct ggaatcgcgc catgaggcgg
     4501 ccgaggcggc tgcccaggcg ctcaaggccg accttggcac ccgcacgacc gcgatccaga
     4561 tccatgaagc cacgattgcg gagcaaagcg cggccatcct gacactcagg accgaccttg
     4621 aggaggcgcg cacccgcatc gccaacctcg aactcgatat cgatgggctg gaaggcgccc
     4681 gtcagaagct gagcgagaat ctggccggtg cacgcgccgt caatcagtcc ctcagcgacg
     4741 atttcagcag cctcaccaaa cgcgccgagg agcaacagat ccgtctggcc tccgccgaga
     4801 cagatatcga gcggctcaac ggtcggctga acgaggcgct gacccgggga cgccagcttc
     4861 aggaagaagg ccactccaag agcgaggaat tgcgccaggc cgcccgcaag ctgcgcgagg
     4921 ccgcttccca gatcggcatt gccatgcgca aactggaggc cgccgaaaag ctggctgaag
     4981 agcgccagca gatgatcgcg caattgcagg acgagcggct cgaactgatc gaccgcggcg
     5041 gcgaatacca gcgcgaacgc gaccgtgaac gggccgatcg gcatgccatc cagtcccagc
     5101 tggataatct gacccgccag atcaacgcca gcaccgccga ggcagaaacc acgcgcggca
     5161 agcagagcga cctggtgcgt gaattgaccc gttctgtcga caatctacgc aacgacaagc
     5221 gtcaggccga tgaagaggtc tcccgcctcc ggatcgagcg gacgatgctg gaaagcgagg
     5281 tcctgcgtct ccgtcgtgcc ctgcaggcgc cccaggaggt cacccgggag atcacccggg
     5341 cagcacaggc agcaccgccg cctgcagccc ctccatccgt tgccgtgccg actccggcgc
     5401 ccgctgtcaa acggccgccg cttcctgcga ccatcggttc gccgctcccc ccgagcgagg
     5461 ctggcgagcg cacctgaccc ggcgcgctca accgcgcccg acaaacggca tcttggtcgc
     5521 catcacggtc atgaacagca cattggcgct ggccggcatg ttggccatgg tcacaatcgc
     5581 atcggcaacg cattgcggat ccatcagcgg ttcgatggca atctcgccat tggcctgcgg
     5641 cacgcccgaa gccatgcgca tcgccatttc ggtagccgca ttgccgatgt cgatctgtcc
     5701 gcaggcgatg tcgtatttgc gcccgtcaag cgccgtcgac cgggtaagac cggagatcgc
     5761 gtgcttggtt gccgtatagg gcgccgaatt cggccgcggt gcataggccg agatggaccc
     5821 gttgttgatg atccgtccgc cgcgcggcgc ctgcgccttc atcacccgca ccgcctcgcg
     5881 cgtgcacagg aacggcccgg tgagattggt atcaaccacc tttttccagt cagccaggct
     5941 gatgtcctca atcggaccgc caccactcat gccggcattg ttgaacagaa gatcgacccg
     6001 cccgaatgtc ctgacggcgc tgtcgaacag ggcgtggacc gaggcttcat cagtgacatc
     6061 ggtgacaacc gcgagcgcct gtccccccgc gctgctgatc atctcggcga cagcctccag
     6121 ctccttcagg cgccggccgg ccagaacgat tgcccagcct gctgccgaca gggcgaggct
     6181 tgacgccttg ccgacgcccg acccggctcc tgtgatgatc gcgaccctgg cttgctttga
     6241 catccttgag accttccggt gagctatttc tgcgggcatt cggcggcata gtgatcgcca
     6301 tactgggcaa agacctgccg cacggcggtg acccggttct taccgtcggt atgcttttca
     6361 acccgcgtaa gatagaggtc ctgaattgga aagttattgt ttccaaaaat gaatttgcca
     6421 cgcgacgact caatgtcggc ctgccggatc gcgtcggcca gcgccgtccg gtccgtcgtc
     6481 ttgcctgacg tggcttcgag cgccttgctg atcagccttg ccgcgtcgaa cccgcgcatc
     6541 gccgctgcgc ccggcgcata gccatactcg gcctcgaagg ccgcgacaaa ggccgcgctg
     6601 gccgctcccg gaacgccctg tgcccagtcg ccgccggaat aggccccgat gacagcatcg
     6661 ccctgaaagg cgacatctgc ctgatcaaac ccgaacgaac cgagcaggac tggcccgctg
     6721 ccagcccccg agcgatgcaa ttcagcgatg aaccgtgcgc ccgccgcgcc gctgacatgc
     6781 aggaacagcg cctggggctt gagaatgtcg atccggttca gcgcatcgga aaagtcgctg
     6841 gcagcaacat cgatcagcag ccgatcggcc acctcgccct tgaaggtgcg ccggaaggcc
     6901 gtagcggcga tttcagcctc ctcgccggcc gtcgaaacga tgatggcgcg cctgaggttt
     6961 cgctcttcgg caaatttggt cagcacttcg atggcctgat cgtcctgcgg agccagcgag
     7021 aagaaattgg gctggcagcg cttgccggcc agcagcgccg gtccggcatg gggcgacaga
     7081 acgatggttc cggccggtgt cgcagccttc agcaccggtg cgatcagctc tgaaaacagc
     7141 ggcccgacga tgaatgccgg cgatttcgct ttcgccatcg cctcggccgc ctgcccggcg
     7201 cgcgctgcgg agcgttcgtc gtcgacaagc tcaatttccg cctcgcttgg ccccagccgg
     7261 ccgccgccga gcttaagccc aagatggaat ccgtcgcgca catgctggcc aagagcggca
     7321 gagggccccg acatggtcag caccagcccg atccgtaccg gctcagcgcc ggcgccggtc
     7381 aagctgtgca ggatgagagc cgcggcgagc agccagcgtc gcatctgtcg tctcccggcc
     7441 cgcctgccgc ccggcaggcc gtttcttgaa cccgtgtctc tcgtatgaca actcattgaa
     7501 ccaatccggc gggagcgaca aggcaaaaat tggcaacaag accgaatctg ccgccgttcc
     7561 tattcaagcc ggacagtcac gctgtctgtt gcaccggcgg catccatcac cgtcacacgg
     7621 gcgaaaccgg ctccgtcggg gttccaatgg gcctgtcgcc gcagttcgtc ctgcaccacg
     7681 ggctgaccat tgaccagcca gatcagcggc ggccgtccac cctgtgcctt cagcgccaga
     7741 tccgtccgtt ccggcccgcc gccgagcccg aggtcgatgc gggcgccatc cggcggatag
     7801 gcgatcctga gtagcggtga cagcgccgcc gaagccgtct tgggcaagtc cttgcgcaga
     7861 tgtctgagcg gcatcggcaa gctgccgttg ctggccatca gcacgccgcg cggcatggac
     7921 agcggttccg gctcgccgcc catccgggcg aaggcatcga acaggaccgg cgccgccaca
     7981 ctccgcccgg tcagccccgg cacc
//
LOCUS       DAOMXG010000114         7925 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_4777_length_7925_cov_9.949809, whole genome shotgun sequence.
ACCESSION   DAOMXG010000114 DAOMXG010000000
VERSION     DAOMXG010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 7925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7925
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4777_length_7925_cov_9.949809"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /gene="serB"
                     /locus_tag="PLQ11_08595"
     CDS             <1..700
                     /gene="serB"
                     /locus_tag="PLQ11_08595"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HRK24996.1"
                     /translation="GPLPVDVVVQPAAGRRKRLLIADMDSTMIGQECIDELADFVGLK
                     DKVSAITERAMRGEIAFEPALRERVALLRGLEAGIVDRILAERITLTPGGRSLVATMR
                     ANGGAAWLVSGGFTLFTEKIAAMLGFDLNRANRLIVEHGRLCGLVEEPILGRQAKLDA
                     LLEARASLGLTASETMAVGDGANDLAMLSAAGLGVAYHAKPQVAAEAHAAINHGDLSA
                     LLYMQGYRRDEFAA"
     gene            complement(842..2365)
                     /locus_tag="PLQ11_08600"
     CDS             complement(842..2365)
                     /locus_tag="PLQ11_08600"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HRK24997.1"
                     /translation="MPTTLLQSVKNGLSASLRRTGRAIALAALGTSLIVTQPLPAFAK
                     GPDSLADLAQSVMDSVVNIKASQKVDQRAVPLPQLPPGSPFEDLFNEFFNRRGGPGGP
                     EGPGGPGRPPQRGSSLGSGFVIDAAGVVVTNNHVIADANEITVVFNDGKELKAELVGK
                     DPKIDIAVLRVKPASPLKAVKFGDSSAMRVGDWVMAIGNPFGFGGSVSAGIVSAKDRN
                     IRSGPYDNFIQTDAAINKGNSGGPLFNMSGDVIGVNTAIVSPTGGSIGIGFAVPSAMV
                     SGVVDQLLKFGETRRGWLGVAIQDVDDAMAESLNIGKARGAFVSNVDPKGPSKTGGIE
                     REDVIIKFDGKEIKSSRDLPRIVADTPVGKEVEVLLLRGGKEVTKTIALGRLEDGEKQ
                     ASAASKPSDSKDGDAGGTKAAGLELGALTPDLRRRFQIKDGVEGVVILRVTQGSKAAE
                     RFVQAGDVIVEVQRTPVKTVADVTKRLQELKKDGRANALFYIANAQGDLRYVTLPVE"
     gene            complement(2472..2645)
                     /locus_tag="PLQ11_08605"
     CDS             complement(2472..2645)
                     /locus_tag="PLQ11_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HRK24998.1"
                     /translation="MGLLLAFEGLLFAAFPSLMRKAMRDASEMPEPAMRMVGVGSAIL
                     GIVLLWVVRSLIG"
     gene            complement(2691..3590)
                     /gene="hflC"
                     /locus_tag="PLQ11_08610"
     CDS             complement(2691..3590)
                     /gene="hflC"
                     /locus_tag="PLQ11_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HRK24999.1"
                     /translation="MAIHPAFLGLAGVVIAGVATSMFVVDQTKQAIVTQFGKPQRVVV
                     QPGLNFKAPFFFESVIQLDRRVLDLETASEEVITSDQKRLVVDAFTRYRIADPLMFFQ
                     ATSGSILAGEDRLKTLLTSALRRVLGGSTFQTVVRDDRAGLVAKVKEQLNRETTALGI
                     SVVDVRIRRADLPEQNSQAIYQRMQTERQREAAEYRAQGVEASERIKAKADRDVVVTR
                     AEAERQAREALGAGDAERTRILAEAYGRDSEFFSFWRSMQAYEAGLKSGDTRMVLSPD
                     SDFFRYLNDPKGRLRSEGDKKQP"
     gene            complement(3592..4713)
                     /gene="hflK"
                     /locus_tag="PLQ11_08615"
     CDS             complement(3592..4713)
                     /gene="hflK"
                     /locus_tag="PLQ11_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HRK25000.1"
                     /translation="MPWSNNGGGPWQPRNNGPWGQGPSGGGGAGGGGPRPPDLEDLLR
                     KGQDRLKQAFPGGQLGGAGVVVVGLVAVLVWLATGIYTVRPNEVGIERVFGKFRSVAS
                     PGLNYNWPAPIGAVDKPTIGVQSVEIGMRSVEGRSGSRRLVPQESLMLTGDENIVDID
                     FTVLWRIDERNPADFVFNLRNNEATVKAVAEASMREVIGKSNALEILTGARLTIESAV
                     QELMQKTLNDYRAGVIIQQVQMQKVDPPQQVINAFRDVQAARADLERAQNEAQAYANK
                     VVPEARGEAARIVQRADAEKEKMVTDAKGEAARFVKLYDEYRKAPDVTRQRLFLETVE
                     RIYGGMDKIILDQSQGGQGVVPYLPLTDLNRRTGQTGAR"
     gene            complement(4796..5302)
                     /locus_tag="PLQ11_08620"
     CDS             complement(4796..5302)
                     /locus_tag="PLQ11_08620"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HRK25001.1"
                     /translation="MPPLVMVAAVARNGVIGNDNQLIWRLKSDLKHFRALTWGLPVIM
                     GRKTYESIGKPLPGRETIVVTREPKRRIEGVEIAHSIDEALFLAGNAMLRMGAPHAIV
                     AGGADIYRAMMPKATRLEITEIALEPQGDALFPPIRREEWRETRREAHRAGPDDEADF
                     SFVSYVRR"
     gene            complement(5365..6159)
                     /locus_tag="PLQ11_08625"
     CDS             complement(5365..6159)
                     /locus_tag="PLQ11_08625"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015951158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HRK25002.1"
                     /translation="MRCYLDLLARVLSEGVAKSDRTGTGTLSLFGAQLRFDLQKGFPL
                     VTTKRLHLKSIIHELIWFLKGDTNIAYLKANGVSIWDEWADANGDLGPVYGRQWRSWP
                     GPNGETVDQIRLVLDEIRRNPDSRRLVVSAWNPVDIPRMALAPCHCLFQFNVADGRLS
                     CQLYQRSADLFLGVPFNIASYALLTHMVAQVSGLQPGDFVHTFGDVHLYSNHLDQARE
                     QLSRQPLPLPRLVLDPSVTDLFAFRAEHVRIEGYQHHAAIRAPVAV"
     gene            complement(6273..6605)
                     /locus_tag="PLQ11_08630"
     CDS             complement(6273..6605)
                     /locus_tag="PLQ11_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2853 family protein"
                     /protein_id="HRK25003.1"
                     /translation="MADVKDYIADIKKYTKTVDEKAVAGMAKTYALVMSRNDSRLVAA
                     SDPDEIQRVVDAFCKKKLGRKESDAALTAVVKAQCEKMKADRSKLRLTVYYLIAEHFQ
                     ALSMFHPK"
     gene            6729..7085
                     /gene="ssrA"
                     /locus_tag="PLQ11_08635"
     tmRNA           6729..7085
                     /gene="ssrA"
                     /locus_tag="PLQ11_08635"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01849"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="alphaproteobacterial-type; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01849"
     gene            7199..7768
                     /locus_tag="PLQ11_08640"
     CDS             7199..7768
                     /locus_tag="PLQ11_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003597772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor
                     SspB"
                     /protein_id="HRK25004.1"
                     /translation="MATDLIRYDVLVQQALRGVVRKVLADSTKDGLPGAHHFYISFDT
                     HAPGVKISPRLREMYPDEMTVVLQHQFWDLAVTDSGFEVSLSFDKIPERMGIPFDAIT
                     GFFDPSVKFGLRFEAVHAEAQSASAPEPAAKDRRAAKPSGEASPRKAKPAKADKPTAE
                     PVTAPAAEEPAEAPGSAEVVSLDAFRKKK"
BASE COUNT         1445 a         2631 c         2388 g         1461 t
ORIGIN      
        1 cggcccgctg ccggtcgatg tggtggtcca gcccgctgcc gggcggcgca agcgcctgct
       61 gatcgccgat atggattcga ccatgatcgg ccaggaatgc atcgacgaac tggccgattt
      121 tgtcgggctc aaggacaagg tctccgccat caccgaacgc gccatgcgcg gcgagatcgc
      181 ctttgaaccg gccctgcgcg agcgcgtcgc gctgttgcgc gggctggaag ccggtatcgt
      241 cgaccgcatc cttgccgagc gcatcacgct cacccccggc ggccggtctc tggtcgcgac
      301 catgcgcgcc aacggcgggg ccgcctggct ggtctccggc ggcttcaccc tgttcaccga
      361 aaagatcgcc gcgatgctgg gctttgatct caaccgggcc aatcggctga ttgttgaaca
      421 cggacggctg tgcggcctgg tcgaggaacc gatcctcggc aggcaggcca agctcgatgc
      481 cctgctggaa gcccgcgcca gtctcgggct gaccgcctcg gagacgatgg cggtgggcga
      541 cggcgccaat gatctggcca tgctctccgc cgccgggctg ggggtcgcct accatgcaaa
      601 gccgcaggtc gcggccgagg ctcatgccgc catcaaccac ggcgatctct ccgccctgct
      661 ctacatgcag ggctatcggc gggacgaatt cgcggcgtaa cagtcggcag tcggcagtcg
      721 gacggaaacg cgcgcaggtg cgatcaagca aaaaggccgg ggacgatccc cggcctttcc
      781 tgtttcacga caaccgactg cctgttcccg attgccgatt gcctattgcc aattgcccgc
      841 gtcactccac cggcagcgtc acatacctca gatcgccctg tgcattggca atatagaaca
      901 gcgcattggc gcggccgtcc ttcttcagct cctgcaaccg cttggtgaca tcggcgacag
      961 tcttgaccgg ggtgcgctgc acctcgacga tcacgtcgcc ggcctgcacg aagcgctcgg
     1021 cggccttgct gccctgcgtc acacgcagga tcacgacgcc ttcgacacca tccttgatct
     1081 ggaagcggcg acgcagatcg ggcgtcagcg cgccgagttc gagacctgcg gccttggttc
     1141 cgccggcatc gccatccttg ctgtcggagg gcttgctagc ggcgctggcc tgcttctcgc
     1201 catcctccag acggccgagg gcaatggtct tggtgacttc cttgccgccg cgcagcagca
     1261 gcacctcgac ctccttgccg accggggtgt ctgcaacgat gcgcggcagg tcacgcgagg
     1321 acttgatctc cttgccatcg aacttgatga tcacatcctc gcgctcgata ccgccggtct
     1381 tggacggtcc cttgggatcg acgttggaga cgaatgcgcc gcgcgccttg ccgatattga
     1441 ggctctcggc catggcatca tcgacatcct ggattgccac gccgagccag ccgcggcggg
     1501 tctcaccgaa tttgagcaac tgatcgacca cgccgctgac catcgccgac ggcacggcaa
     1561 agccgatgcc gatggagccg ccggtcggcg agacgatagc cgtgttcacg ccgatcacat
     1621 cgccgctcat gttgaacagc ggcccgcccg aattgccctt gttgatggcg gcatcggtct
     1681 ggatgaaatt gtcatagggg ccggagcgga tgttgcgatc cttggccgag acgataccgg
     1741 cagagaccga cccgccgaag ccgaacggat tgccgattgc catcacccag tcgcctacgc
     1801 gcatcgcgct cgaatcgccg aacttgaccg ccttgagcgg gcttgccggc ttgacgcgca
     1861 gcacggcgat atcgatcttg ggatccttgc caaccagctc ggccttcagt tccttgccgt
     1921 cgttgaacac caccgtgatc tcgttggcgt cagcaatgac gtggttgttg gtcaccacca
     1981 cgccggctgc atcgatgacg aagcccgaac cgagagagga gccgcgctgc ggcggccgcc
     2041 ccggaccgcc gggaccctca ggccccccag gtccgccacg gcggttgaag aactcgttga
     2101 acagatcctc gaatggtgag cccggcggaa gctgcggcag cggaacggcg cgctggtcca
     2161 ccttctgcga cgctttgatg ttgaccaccg aatccatgac gctctgcgcc agatcggcca
     2221 gagaatccgg ccccttggca aaggccggca acggctgcgt gacgatcagg ctggttccga
     2281 gtgcggcaag cgcgatcgcg cgccccgtcc gccgcagcga cgcggacaag ccattcttga
     2341 cgctctggag aagtgtggtc ggcatgggta tccctctcat aagtccggtc gcccgagacc
     2401 gcatctgatg accgccgaat tgcggcggaa acgcgacgaa accgccttaa ctttctgtaa
     2461 tcccggcgct ttcagccgat caggctgcgc accacccaca gcagaacgat cccgagaatg
     2521 gcggagccga caccgaccat gcgcatggcc ggctccggca tttccgaagc atcccgcatc
     2581 gctttgcgca tcagcgacgg gaacgccgca aacagcaacc cttcgaaggc aagcagcaac
     2641 ccgattgcaa aaacaacatc cctgatggct gcctcccccg tttgcgtgct ctatggctgc
     2701 ttcttgtccc cttctgaccg tagcctgccc ttgggatcat tcaaatagcg aaaaaagtcg
     2761 ctgtcgggtg aaagcaccat tctggtgtct ccgctcttca ggcccgcttc ataggcctgc
     2821 atcgagcgcc agaacgagaa gaactcggag tcgcgcccat aggcttcggc gaggatgcgc
     2881 gtgcgctcgg catcgccagc gcccagcgcc tcgcgcgcct gccgctcggc ttcggcacgg
     2941 gtgacaacca cgtcgcggtc ggccttggcc ttgatccgct ccgaagcttc cacgccctgc
     3001 gcgcgatact ccgccgcctc gcgctggcgt tccgtctgca tccgctgata gatcgcctgg
     3061 ctgttctgct ccggcagatc ggcacggcgg atgcgcacat cgacgacgga gatgcccagc
     3121 gctgtggtct cgcgattgag ctgctccttg accttggcga ccaggccggc acggtcatcg
     3181 cgcaccacgg tctggaaggt cgagccgccc agcacgcgcc tcagcgccga agtcagcagc
     3241 gtcttgagcc ggtcctcgcc agccaggatc gaaccggagg tcgcctggaa gaacatcagc
     3301 ggatcggcga tgcgatagcg ggtgaaggcg tccaccacga gccgcttctg atcggaggtg
     3361 atcacttctt ccgacgccgt ttcaagatcg agaacgcgcc gatccagctg gatcacgctc
     3421 tcgaagaaga acggcgcctt gaagttcagc cccggctgga cgacgacgcg ctgcggcttg
     3481 ccgaactggg tgacgatggc ctgtttggtc tggtcgacca cgaacatcga ggttgcgacg
     3541 ccggcgatca cgacgccggc aagtccgagg aatgcaggat gaatggccat gtcagcgcgc
     3601 tcccgtctgg ccggtgcggc ggttgagatc ggtcagcggc agatagggca caacaccctg
     3661 ccccccctgg ctctgatcga ggatgatctt gtccatgccg ccatagatcc gttccaccgt
     3721 ttcgaggaac aggcgctggc gggtcacatc cggcgccttg cggtattcgt catagagctt
     3781 gacgaaacgg gcagcttcgc ccttggcatc ggtgaccatc ttttcctttt cggcgtcggc
     3841 ccgctgcacg atgcgggcgg cctcgccgcg cgcttccggg acaaccttgt tggcataggc
     3901 ctgggcttcg ttctgcgcac gctcgagatc ggcgcgggcg gcctgcacat cgcggaaggc
     3961 gttgatgacc tgctgcggcg ggtccacctt ttgcatctgc acctgctgga tgatcacgcc
     4021 ggcacggtag tcgttcagcg tcttctgcat aagttcctgc accgcgcttt cgatggtcag
     4081 gcgcgcacct gtcaggatct ccagcgcgtt ggacttgccg atgacctcgc gcatcgaggc
     4141 ttccgccacg gctttgaccg tcgcctcatt gttgcgcagg ttaaagacga aatcggccgg
     4201 gttgcgctcg tcgatacgcc acaggacggt aaaatcgata tcgacgatgt tctcgtcgcc
     4261 ggtcagcatc aggctttcct gcggcaccag gcgccggctg cccgagcgcc cttccaccga
     4321 gcgcatgccg atctccaccg actggacgcc gatggtcggc ttgtcgaccg ctccgatcgg
     4381 cgccggccag ttgtagttga ggccggggct ggcaacagaa cggaacttgc cgaaaacccg
     4441 ctcgataccg acctcgttcg gccgcaccgt atagatgccg gtcgccagcc agaccagaac
     4501 ggcgacaagc ccgacgacaa ccacaccggc accgccgagc tgtccgccgg gaaatgcctg
     4561 cttcagccga tcctgtccct tgcgcagcag atcctcaaga tccggcgggc gcggaccgcc
     4621 tccgccagcg ccgccaccac ccgatggtcc ctgaccccag ggtccgttgt tgcggggctg
     4681 ccatggcccg ccgccattgt tgctccaggg catgaatgtc ctcgttcagg gcaaaacaca
     4741 aaacgcgata taggaacgct tggccaccag cgcaatgcac cgcgagcaag aagcttcagc
     4801 gccgcacata actgacaaag gaaaaatcgg cctcatcatc cggacccgcc ctgtgcgcct
     4861 cgcgccgggt ttcacgccat tcctcgcgac ggattggcgg aaacaatgca tcgccctgcg
     4921 gctccagcgc gatttcggtg atttcgaggc gcgttgcctt gggcatcatg gcccggtaga
     4981 tgtccgcccc tccggcgacg atcgcgtggg gagcgcccat gcgcagcatg gcattgccgg
     5041 ccaggaacag cgcctcatca atcgaatggg caatctccac cccctcgatg cgtcgcttcg
     5101 gctcgcgcgt gaccacaatt gtctcgcgcc ccggcagggg cttgccgatc gattcatacg
     5161 tcttgcgccc catgatcacc ggcagacccc aggtgagcgc cctgaaatgc ttgagatcgc
     5221 tcttcaggcg ccagatcagc tggttgtcat tgccgatcac gccattgcgg gccacggctg
     5281 caaccatcac cagcggcggc atgtcatatc ctcctcaggc ttcatgcgat ccgactatgg
     5341 cggctggctc tcgttcaggc aacgctacac cgccaccgga gccctgattg cagcatggtg
     5401 ctgataccct tcgatccgca catgctcggc gcggaaggca aacagatccg tcaccgaggg
     5461 atcgagcacc agccgcggca gaggcagcgg ctgacgcgaa agctgctcgc gcgcctgatc
     5521 gaggtggttc gaataaagat ggacatcgcc gaacgtgtgg acgaaatcgc ccggctgaag
     5581 cccgctcacc tgcgccacca tatgcgtcag cagggcatag gaggcgatat tgaacgggac
     5641 gccgagaaac agatcggcgg agcgctggta gagctgacac gacagccgcc cgtcggcgac
     5701 gttgaactgg aacaggcaat ggcacggggc cagcgccatc cggggaatat ccaccgggtt
     5761 ccaggccgag acaaccaggc ggcgcgaatc cggattgcgc cggatctcgt caagcaccag
     5821 acggatctgg tcgaccgtct cgccgttcgg tcccggccag ctccgccatt gcctgccata
     5881 gaccgggccg agatcgccgt tggcatcggc ccattcatcc cagatgctga cgccattggc
     5941 cttgagatag gcgatgttgg tgtcaccctt gaggaaccag atcagctcgt ggatgatcga
     6001 cttgaggtgc agccgcttgg tcgtgaccag cggaaagccc ttctgaagat cgaacctgag
     6061 ctgggcgccg aacagcgaca gcgtacccgt gccggtgcga tcgctcttgg cgacgccctc
     6121 cgacaaaacc cgcgccagca gatcgagata gcagcgcatg accccccaac ccgttcagaa
     6181 ccgcggctgt ccatccattg caaaaacagc cgggcgcaga acggctgtcc cgtcctgcgc
     6241 ccgcagcagc atagaccgat tcggcgatcc gcttacttcg gatggaacat cgacagcgcc
     6301 tggaaatgct cggcgatgag gtaatacacc gtcaggcgca gcttggagcg gtcggccttc
     6361 atcttctcgc actgggcttt gaccacggcc gtcagagccg catcggattc cttgcggccg
     6421 agcttcttct tgcagaaggc atcgacaaca cgctggatct catcgggatc ggaggcggcg
     6481 accagacgcg agtcattgcg gctcatcacc agcgcatagg tcttggccat gccggccacg
     6541 gccttctcgt ccacggtctt ggtgtatttc ttgatgtcgg caatatagtc tttgacgtcg
     6601 gccatgtcct gtctcccacg ggaatcatca aaccccgcta agaccttata cttctcgtga
     6661 aatttcgtcc agagccgctt tgcctctatt tcagcaaaag gagggattgg cgtcctcgct
     6721 cctggcgtcc ccttccaaat ctgccgtccc tgccctatat tcgtttcgtt accggcgacg
     6781 gtgactatgg cgataaacgg ctatcgtaat aaacccatcg gacccggggg cagtacccgg
     6841 cgcctccacc caactccagt cgcggctggg gtttggcggg ggcgaaacag gatcgacgag
     6901 ggcgtaaagg gtggactttt gcccggcatg ataccgccgt tatcgggtca aacctatagt
     6961 tgcgaatgac aactatgctc cggttgctca ggccgcgtaa tgcggtttga aagaccgact
     7021 caaagccctt ctggttagca cctgaaggcg gggttcgcag gcacctggca acagaagcct
     7081 gcacttctca ccattgccgc aacctgccat gcaccacgcc cggtcgtgcc gattcccacc
     7141 cggcccggaa tcgcgtatgc aacccgtgtg ggcttccgcc tgaggggtgt gcaaactgat
     7201 ggcaaccgac ctgatccgtt atgatgtgct cgtccagcag gcgctgcgcg gcgtggtgcg
     7261 caaggtgctt gccgacagca ccaaggacgg gctgcccggt gcccatcatt tctatatttc
     7321 cttcgacacc catgcgcccg gcgtaaagat ctctccgcgt ctgcgcgaga tgtatccgga
     7381 cgagatgacg gtggtgctcc agcaccagtt ctgggatctg gcggtgacgg acagtggttt
     7441 tgaggtgtcg ctgtcctttg acaagatccc cgagcggatg ggcattccct tcgatgcgat
     7501 caccggcttt ttcgacccct cggtgaagtt cggtttgcgg ttcgaggccg tgcatgccga
     7561 ggcgcaaagc gcttctgcgc cggaaccggc agccaaggat cgtcgcgccg ccaagccgag
     7621 tggcgaggct tcgccccgca aggccaaacc ggcgaaagcc gacaagccaa ccgctgaacc
     7681 tgtgaccgca ccggctgccg aggagcctgc cgaagccccg ggttctgccg aggttgtcag
     7741 cctcgacgct ttccggaaga agaaatagcc ccccattcta tcgtgcctgc atcgcccgcc
     7801 gattgcgccg gttcaatgcg gcctggcccg gctttgctag actcgccccc ggataaaacg
     7861 gagggtttca tgtcaaagcc attgattgcc gtcacatcgg ccgcgttcgc cgccagcgcc
     7921 ctgat
//
LOCUS       DAOMXG010000115         7909 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_4792_length_7909_cov_8.512223, whole genome shotgun sequence.
ACCESSION   DAOMXG010000115 DAOMXG010000000
VERSION     DAOMXG010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 7909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7909
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4792_length_7909_cov_8.512223"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1223)
                     /locus_tag="PLQ11_08645"
     CDS             complement(<1..1223)
                     /locus_tag="PLQ11_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase D protein"
                     /protein_id="HRK25005.1"
                     /translation="MRIPHFWEPEETVGSAWHDLVRNWGAEPAFEAARVAYPDVATSV
                     GLVYRAIGGAVGTEILPSAATVARSRVTGRRKLGHDVDHVTRASYDGQTLALPAAVAL
                     FEDAALNRKLYIWLAALAAFVETPFEVPGTPLADDVAMLRACHAAERRLTAALPGFRA
                     MRAELAATLLAQRPQPKLPPVEAELEAAIRALLVGSMPQANRFSVLIAGDGDPAALAT
                     PEDYRPFRPVALWPLLSPERKADRVPRSDDQADEGGPPPGEGSEKTLRAQRHDADQAN
                     RKDSLILHRFESIMSWVEFLNINRAIEDEDEDNAKKAAEDLDEVSLAKNSRSAKTKLK
                     FHLDLSPRDVERERLIGQATYPEWDWKSGSYLPDHVSVEERTAEEKDSDAFDRPRTRR
                     RIMRYTTSIAPRPITP"
     gene            complement(1262..2074)
                     /locus_tag="PLQ11_08650"
     CDS             complement(1262..2074)
                     /locus_tag="PLQ11_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006938177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HRK25006.1"
                     /translation="MNAVSSPETALATSVPYYRPVGNECAVFEAAFRNRLPLLIKGPT
                     GCGKTRFVAHMAARLAAGLTTVSCHDDLTASDLTGRWLIKGGETVWQDGPLTRAVRQG
                     GICYLDEIVEARKDVTVVLHPLTDDRRILPLERTGETVVAPDNFMLVVSYNPGYQNIL
                     KSLKPSTRQRFMAIEFSYPPPAVEAEVLVTETGVKPAVADRLVALAGHIRKLKGRDLE
                     EGASTRLLVYAASLIASGMKINEAIDVALVEPLTDDADVKRALGDLVVAVFG"
     gene            complement(2148..3518)
                     /locus_tag="PLQ11_08655"
     CDS             complement(2148..3518)
                     /locus_tag="PLQ11_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018327474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HRK25007.1"
                     /translation="MKYETQAIAKWYWFAALALFAVQVLMGLVAGYIYVQPNFLSELL
                     PFNIVRMLHSNALIVWLLLGFFGAAYYIIPEESEREIHSPAIAYFQLILLVVGTLGAV
                     LTYMFNLFEGNVLLGKQGREFLEQPLWVKLGIVVAALAFLYNITLTVLAGRKTAITNV
                     LLLGLWGIAVFFLFGLYNPSNLALDKIYWWWVVHLWVEGVWELVMASILAFIMLKLTG
                     VDREVIEKWLYVIVATSLFTGVLGTGHHYYWIGAPSYWTWIGSIFSSLEVIPFFAMVL
                     FTFMMVWKGKRNHPNKAALLWALGCSTLAFFGAGVWGFMHTLHPINYYTHGTQVTAAH
                     GHLAFFGAYVSLNLAIMSYAFPSIFRINAYNQVLNMVSFWIMSSAMAFMTFVLTFAGA
                     VQTHLQRVLGMGYMEVQDQLSLFYWMRFGAGLAVVIGALLFIYAILGPKKEATGLEAA
                     ALSPAE"
     gene            complement(3545..3997)
                     /locus_tag="PLQ11_08660"
     CDS             complement(3545..3997)
                     /locus_tag="PLQ11_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002966338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRK25008.1"
                     /translation="MSELLTKSAARNIFYGGSLFFFLIFVGLVVHTHNYIRTVSTDES
                     KLTPAVVNGKHVWEKHACINCHSLIGEGAYFAPELGNVWQRYSGDKDPKAAREALKAW
                     MINQPTGIAGRRQMPKFAFTGTELDDLISFLEWSSTIKTNNWPPNKAG"
     gene            4195..5181
                     /locus_tag="PLQ11_08665"
     CDS             4195..5181
                     /locus_tag="PLQ11_08665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HRK25009.1"
                     /translation="MDLDDLNPAGGLGAISKLPVFLDLAGRKAVLAGNTPGLAWKAEL
                     IAAAGADVTVLAPEPSEELAALVARGAPAGSLRLVERVWSPADLDGAAIAVADLETLG
                     EAAAFKAAGDAAGILVNTIDKGATCDFYFGAIVSRSPIMIGATTDGTAPILGQAIRRA
                     IELAVPGWLAPWADFARAIRPEVKRRLAPGVQRRSFWETFTERAMSAPLDDAGRQDVM
                     HAIDRAGMLARSGGGHRHDLVAPATTDALTLADVKRLQSADLIVEEPGATTAQAFYRR
                     EATRLVIGQAGRAGEIAEDEIEALATRTIESGRRIVVVRLPRPGSVDLRIAS"
     gene            5178..5330
                     /locus_tag="PLQ11_08670"
     CDS             5178..5330
                     /locus_tag="PLQ11_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25010.1"
                     /translation="MTLRHTGLIIAPILIAAMAVLMHRKGALPRGGLILVLAVVAAIT
                     AVLMST"
     gene            5341..6204
                     /locus_tag="PLQ11_08675"
     CDS             5341..6204
                     /locus_tag="PLQ11_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRK25011.1"
                     /translation="MRRLLILGHGYCARRFVALHGAAFGSIVATRRTGSTEAVDRPVS
                     ALDFNGQPDEAIGAAFDEASHILATAPPGAEGDPFLPVLAAMAGADRASRSVVYLSTI
                     GVYGDRGGDWVDATAAPNPASERSRRRLAAEQDWAGFAKKTGASLAVLRLPGIYGPGR
                     SVIDDLLAGSARRIVKPGQVFNRIHVDDVAEAIGRAFERRFDGIVDIVDDAPAPPQDV
                     VTYAAGLLGLAPPPPVAFDAASLTPMARSFYSENKRVSNARTRALLGWRPNFPTYREG
                     LAAILASRGSE"
     gene            complement(6235..>7909)
                     /locus_tag="PLQ11_08680"
     CDS             complement(6235..>7909)
                     /locus_tag="PLQ11_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25012.1"
                     /translation="PSTPPATPPPETASISTTTGDMEHALLGGVASVAGGYAGEWAAG
                     QVAEQILDGANLPDWSAAAARTATNSLVSGAIRGDVEGGLLSGAMTVAGAALTPDVPL
                     ADADKPNELGLTPAQMNRIGSTIGDLARGYITGGERGLAGAAVSLVGGEIVEALARGD
                     ATPTATAGDGANGAVPTAPANDPLLDFLVEQGLIDPGTGLATDPGSLEERMAADPMFA
                     ALVNAFSNPYGPVASPLLAEDFKPWARSVDGELFIDSNQLLPGVGYELPDTGDEATRL
                     AALEPADLPGSTRVDSLPSGWGINMRPWVAGTITHRLFASEMNLLNPLAGPMLVASDG
                     RARYMDLILRNDVFELKPISWQFDAGRRTSADIQVTDYVNAMNNNVGRLAFFGSNEDG
                     EPLRYQPGRSDQILRPNTPDGGMRLNSVLYDGKDFFSVTLYPDHRERSGQRTGLIFYE
                     LNPMARTAELEKRLEQAREQSLVDRYGGFGFIGLEGSGRQAEQLPSSSMVQQFIDAMQ
                     PRQRPDDPLAPFEGRPTRPSQPAPQIPIILPGLPVRIPGTPPVIPPPRP"
BASE COUNT         1372 a         2533 c         2629 g         1375 t
ORIGIN      
        1 ggcgtgatgg gcctgggggc gatgctggtg gtgtatcgca tgatgcgccg gcgggtgcgc
       61 ggccggtcga aggcgtcgct gtccttctcc tcggcggtgc gctcttccac cgagacatga
      121 tccggcagat agctgccgct cttccagtcc cattccggat aggtcgcctg cccgatcagc
      181 cgctctcgct cgacatcgcg cggggaaaga tcgaggtgga acttcagctt ggtcttggcc
      241 gagcgggaat tcttggccag cgagacctcg tcgagatcct cggccgcctt ttttgcgttg
      301 tcctcgtcct catcctcgat ggcgcggttg atgttgagga actcgaccca ggacatgatc
      361 gattcaaacc ggtgcaggat gagactgtcc ttgcggttgg cctgatcggc atcgtggcgc
      421 tgggcgcgca gggtcttttc ggacccttcg ccggggggcg gtccgccctc atcggcctga
      481 tcgtcagagc gcggaacgcg gtccgccttg cgctccgggc tcagcagcgg ccacagcgcg
      541 accgggcgga aggggcggta atcctccgga gttgccagtg cggccggatc gccgtcgccg
      601 gcaatgagca cggaaaagcg gttggcttgc ggcatgctgc ccaccagcag cgcgcggatc
      661 gccgcttcaa gctcggcctc gaccggcggc agtttgggtt gcggccgctg ggccagcagc
      721 gtcgcagcca attcggcgcg catggcgcga aaccccggca aggccgctgt cagccgccgt
      781 tcggcagcat ggcaggcgcg cagcatcgcc acatcgtcgg ccagcggcgt tcccggcacc
      841 tcgaatggcg tttcgacaaa ggccgccagc gctgccagcc agatgtagag cttgcggttg
      901 agcgcggcat cctcgaacag ggccactgcg gcaggcaatg ccagcgtctg gccgtcatag
      961 ctggcgcggg tcacatgatc gacatcgtgg ccaagcttgc gccggccggt gacgcggctt
     1021 ctggcgaccg tcgcagccga gggcaggatc tcggttccga ccgcaccgcc gatggcacgg
     1081 tagaccagcc cgaccgaggt ggccacatcc ggataggcga cgcgcgccgc ctcgaacgcc
     1141 ggttcggcac cccagttgcg caccagatca tgccatgcag agccgacggt ttcctcaggc
     1201 tcccagaaat gcggaatgcg catggcgctc tcccctcgct ggccggtccg gcgccgggct
     1261 gtcagccgaa taccgccacc acgagatccc ccagggcgcg cttgacatcg gcatcgtcgg
     1321 tcaagggctc gacaagtgca acgtcaatgg cctcgttgat cttcatcccc gaggcgatga
     1381 ggctggcggc atagaccagc aggcgggtcg aggcgccctc ttccagatcg cggcccttga
     1441 gcttgcggat atggccggcc agcgcgacaa gccgatcggc caccgcaggt ttgacgcctg
     1501 tctcggtcac cagaacctcg gcctccaccg ccggcggcgg gtaggaaaac tcgatcgcca
     1561 tgaagcgctg gcgggtcgag ggcttcaggc ttttgagaat gttctggtag ccggggttgt
     1621 aggagaccac gagcatgaaa ttgtcgggcg cgacaacggt ttcgccggtg cgttccagcg
     1681 gcaggatgcg gcggtcgtcg gtcagcggat gcaggaccac ggtcacgtcc ttgcgcgcct
     1741 cgacgatctc atcgagatag cagatgccgc cctggcgtac agcgcgggtg agcgggccat
     1801 cctgccagac ggtttcgccg cccttgatca gccagcggcc ggtcaggtcg gaggctgtca
     1861 gatcatcatg gcaggagacc gtggtcaggc cggcggcaag ccgtgccgcc atatgggcga
     1921 caaagcgggt cttgccgcat ccggtcggtc ccttgatcag cagcggcagg cgattgcgga
     1981 aagcggcttc gaacaccgcg cattcattcc cgaccggccg gtaatagggc acgctggttg
     2041 ccagcgccgt ttcgggtgag gaaaccgcat tcatcaagga gcctcgcagc tttggttggt
     2101 cgaaagcagg cgggccacac ctctccggcg cggcccgccc cgtctggtca ttcggccggc
     2161 gacagggcag cggcctcaag tccggtcgct tccttcttcg ggccaaggat ggcgtagatg
     2221 aacagcagcg cgccgatcac cactgcaagc cccgccccga agcgcatcca gtagaacagc
     2281 gacagctggt cctgaacctc catgtagccc atgccgagaa cgcgctgcag atgggtctgc
     2341 accgcaccgg cgaaggtgag cacgaaggtc atgaaggcca tggcgctgga catgatccag
     2401 aacgacacca tgttcagcac ctggttatag gcgttgatgc ggaagatcga cgggaaggcg
     2461 taggacatga tcgcgagatt cagcgacaca taggcgccga agaaggcaag atgtccgtgg
     2521 gccgccgtca cctgcgtgcc gtgggtgtag tagttgatcg gatgcagcgt gtgcatgaag
     2581 ccccacacgc ccgcgccgaa gaaggccagg gtcgagcagc ccagcgccca cagcagcgcc
     2641 gccttgttgg gatggttgcg cttgcccttc cacaccatca tgaaggtgaa cagcaccatg
     2701 gcgaagaacg ggatcacctc cagcgaggag aagatcgagc cgatccaggt ccagtagctc
     2761 ggagcgccga tccagtaata gtgatggccg gtgcccagaa cgccggtgaa cagcgaggtg
     2821 gcgacgatga cataaagcca cttctcgatc acctcgcggt caacgcccgt cagcttcagc
     2881 atgatgaagg cgaggatcga ggccatgacc agttcccaca cgccctcgac ccagagatgg
     2941 acaacccacc accagtagat cttgtcgagc gcaagattgc tcggattgta gagcccgaac
     3001 aggaagaaca cggcgatgcc ccacagcccc agcagcagca cgttggtgat ggcggtcttg
     3061 cggccggcca gcacggtcag cgtgatgttg tagaggaagg cgagcgcggc gacgacgatg
     3121 ccgagcttga cccacagcgg ctgctcaagg aactcgcgcc cctgcttgcc aagcagcacg
     3181 ttgccctcga acaggttgaa catgtaggtg agcaccgcac ccagcgtgcc gaccaccagc
     3241 aggataagct ggaaataggc gatggccggc gagtggatct cacgctcgga ttcttcggga
     3301 atgatgtagt aggcagcgcc gaagaagccg agcagcagcc agacgatcag cgcgttggaa
     3361 tgcagcatcc ggacgatgtt gaacggcagc agctccgaca ggaagttcgg ctgcacgtag
     3421 atgtagccgg ccaccaggcc catgagcacc tgcacggcga acagggcgag cgcggcgaac
     3481 cagtaccatt tggcgatagc ttgggtttcg tatttcatgt gagtgatccc cggaacttgg
     3541 ttgctcagcc ggccttgttc ggcggccagt tgttggtctt gatggtgctc gaccactcga
     3601 ggaacgagat cagatcgtcg agttccgtgc cggtgaaggc gaatttcggc atctgccggc
     3661 gcccggcgat cccggtcggc tggttgatca tccaggcctt cagcgcttca cgggccgcct
     3721 tggggtcctt gtcgccgctg tagcgctgcc agacgttgcc gagttcggga gcaaaatagg
     3781 cgccctcacc gatcaggctg tggcaattga tgcaggcatg cttttcccag acatgcttgc
     3841 cgttcaccac cgccggggtg agtttgctct cgtcggtcga gaccgttcgg atgtagttgt
     3901 gcgtgtgcac gaccagcccg acgaaaatca gaaagaagaa gagggatccg ccgtagaaga
     3961 tgtttcttgc cgcggatttc gtgagaagct cgctcatgcg ccaccccgat gttagaccag
     4021 accatcgtca gggatagtgt cgcagccggc cgcgtccttg atgaatggta atcgccccgg
     4081 cttgagccgc atgccggccg gatggcttgt caaagatcaa ggctggcgcc gaccatccgg
     4141 cgtcattctc caccggacgg caaaagcaac gactttcccg ctaaggaccc ttccatggac
     4201 ctcgatgatc tcaatccggc aggcggcctc ggggccattt ccaaactgcc ggtgtttctc
     4261 gatctggccg gacgcaaggc ggtgctggcc ggcaataccc ccggcctggc gtggaaggcc
     4321 gaactcatcg ccgccgccgg cgccgatgtg accgtgctgg cgcccgaacc gtccgaggaa
     4381 cttgccgcgc tggtggcgcg gggggctccg gcgggcagtc tgaggctggt ggagcgggta
     4441 tggtctccgg ccgatctaga cggcgccgcc atcgcggtgg ccgatctgga gaccctgggc
     4501 gaagctgccg ccttcaaggc agcaggtgat gccgccggca ttctggtcaa caccatcgac
     4561 aagggggcga cctgcgattt ctatttcggc gccatcgtca gccggtcgcc gatcatgatc
     4621 ggcgccacca ccgacggcac cgccccgatc cttgggcagg cgatccgccg cgccattgaa
     4681 cttgccgtgc ccggctggct ggcgccctgg gcggattttg cccgggcgat ccgccctgag
     4741 gtcaagcgcc ggctcgcccc gggggtgcag cggcgcagtt tctgggagac cttcaccgaa
     4801 agggcgatgt cggcgccgct tgacgatgcc ggtcgccagg acgtgatgca tgccatcgac
     4861 cgcgccggca tgctggcgcg ctcgggcggc ggccatcgcc atgatctggt tgcgccggcc
     4921 acgaccgacg ccctgaccct ggccgatgtc aaacgcctgc aatcggccga tctgatcgtc
     4981 gaggaaccgg gagcgaccac cgcgcaggcc ttctatcgcc gcgaggcaac gcggcttgtt
     5041 atcggtcagg cgggacgggc cggtgagatc gccgaggacg agatcgaggc cctcgccacc
     5101 aggaccatcg aatcgggccg ccgcatcgtt gtggtgcgtc tgcccagacc cggatcggtt
     5161 gatttgcgga tcgcttcatg accctgcgcc ataccggact gatcatcgcc cctatcctga
     5221 tcgcggccat ggccgtgctg atgcatcgca agggcgccct gccccgcggc gggctgatcc
     5281 tggtgctggc ggtggtcgct gccatcaccg ccgtgctgat gtcgacctag agaatagagc
     5341 atgcggcgcc tgctgatcct cggacacggc tattgcgcgc ggcggttcgt ggcgctgcat
     5401 ggcgctgcgt tcggctcgat tgtcgcgacc cggcggaccg gctcgacgga ggcggtggac
     5461 cgtccggtct cggccctcga cttcaacggc cagccggacg aggcaatcgg ggcggcattc
     5521 gatgaagcca gccatatcct ggcgacggcg ccgcccggtg ccgaaggcga tccgttcctg
     5581 ccggtgctgg ccgccatggc cggggccgat cgcgccagcc ggtcggtcgt ctatctctcc
     5641 accatcggcg tctatggcga tcggggcggc gactgggtcg atgccaccgc tgcacccaat
     5701 ccggccagcg agcgcagccg ccgccgtctg gcggccgagc aggattgggc agggtttgcg
     5761 aaaaagaccg gcgccagcct tgccgtgctg cgccttcccg gcatttatgg ccccgggcgc
     5821 agcgtgatcg acgacctgtt ggccggttcg gcgcggcgga tcgtcaagcc cgggcaggtg
     5881 ttcaaccgca tccacgtcga tgatgtcgcc gaggcgatcg ggcgggcttt cgaacggcgg
     5941 ttcgatggta ttgtcgatat cgtcgatgac gccccggcac cgccccagga tgtcgtcacc
     6001 tatgccgccg gcctgctggg cctggcgccg ccgccacccg tcgcgttcga tgcagccagc
     6061 ctgacgccga tggcgcgcag tttctattcg gaaaacaagc gtgtttccaa cgcgcgcacc
     6121 cgggccctgc tcggctggcg gccaaacttt ccgacctatc gcgagggcct ggccgccatt
     6181 ctcgcctcac gtggaagcga atgacgcggg tcgccggcgc ctgatcatga acattcatgg
     6241 acgcggaggc ggtatcaccg gtggtgtgcc cggaatccga acgggcagtc ccggaaggat
     6301 gatcggaatc tgcggagccg gctgtgacgg ccgcgtcggg cgcccctcga acggtgccag
     6361 cggatcgtcc ggccgctgcc gcggctgcat cgcgtcgatg aactgctgga ccatggagga
     6421 tgacggcagc tgctcggcct gtcggccgga gccttccagc ccgatgaaac cgaatccccc
     6481 atagcgatcg acaaggctct gctcgcgggc ctgttccagc ctcttctcca gttcagccgt
     6541 ccgggccatc ggattgagtt cgtagaagat cagtccggtg cgctggccgc tccgctcccg
     6601 gtggtcggga tagagcgtca ccgagaagaa gtccttgcca tcatagagca cggaattgag
     6661 acgcatgccg ccgtccgggg tattgggccg caggatctgg tcgctgcgtc ccggctgata
     6721 gcgcaacggc tcgccgtcct cgttgctgcc gaagaaggcg aggcggccga cgttgttgtt
     6781 cattgcattc acatagtcgg tcacctgaat atcagccgat gtccgccggc ctgcatcgaa
     6841 ctgccagctg atcggtttca gttcgaaaac gtcgttcctt aggatgagat ccatgtagcg
     6901 ggcccggcca tcgctggcga ccagcatggg cccggccagc ggattgagca gattcatctc
     6961 gctggcgaac agccggtggg tgatcgtccc ggcgacccag ggacgcatgt tgatgcccca
     7021 gcccgacgga aggctgtcca cccgggtgct gccgggaaga tcggccggtt caagggcggc
     7081 aagccgggtc gcctcgtcgc cggtgtccgg caattcatag ccgacgccgg gcagcaactg
     7141 atttgaatcg atgaaaagtt ccccgtcaac agatcgcgcc cacggtttaa agtcctccgc
     7201 aagcagtggt gaggcgaccg ggccgtaagg gttcgagaag gcgttgacca gcgcagcgaa
     7261 catcggatcg gcggccattc tttcctcgag cgagcctgga tcggtggcca gtccggtgcc
     7321 ggggtcgatg aggccttgct caaccagaaa atcgagcagc ggatcattgg cgggcgcggt
     7381 cgggacggca ccgttggctc catctcccgc cgttgcggtc ggtgttgcat cgccacgcgc
     7441 cagcgcctcg acaatctccc cgccgaccag cgaaaccgcc gcgccggcaa ggccgcgttc
     7501 gcctccggta atgtagccgc gggcaagatc gccaatggtc gagccgattc tgttcatctg
     7561 cgctggtgtg agacccagtt cgttcggctt gtcggcgtcg gcgagcggca cgtccggggt
     7621 cagggccgca ccggcgaccg tcatcgcgcc gctgagcaat ccgccctcaa catcgccgcg
     7681 aatggcaccg gataccagcg aattggtcgc cgtccgcgcc gccgcagcgc tccaatccgg
     7741 cagattggcg ccatcgagaa tctgctcggc gacctgcccg gccgcccatt caccggcata
     7801 gcctccggca acgctggcaa ccccgcccag cagcgcatgc tccatatcac cggtggtcgt
     7861 ggatattgaa gcagtttctg gtggaggcgt cgctggtggt gttgatggg
//
LOCUS       DAOMXG010000116         7680 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_4970_length_7680_cov_9.677770, whole genome shotgun sequence.
ACCESSION   DAOMXG010000116 DAOMXG010000000
VERSION     DAOMXG010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 7680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7680
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4970_length_7680_cov_9.677770"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="PLQ11_08685"
     CDS             complement(<1..218)
                     /locus_tag="PLQ11_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HRK25013.1"
                     /translation="MTLRILIMEGNDAEGRARQKSRLGRTASDNYAGCIRFLAPDVAT
                     DICQPADEDFVIPDLSRIRSYDGVVITGS"
     gene            335..1231
                     /locus_tag="PLQ11_08690"
     CDS             335..1231
                     /locus_tag="PLQ11_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRK25014.1"
                     /translation="MISRLLTNPFLLLTLTALAWAGNTIAGRLAVGEVSPMVLTTLRW
                     VISFTLMGLFVRADVARALPLFRQRPLYLTLMGIIGFTGFNAIYYVAAHYTSAANLGI
                     MQSSTPLFVMIGAVLLGQSQVNLAQVIGLAIGLAGVLIVVTTGRWEVLHNLAFNIGDL
                     LLILISAMYAGYSLGLRHRPQGVPGLTFFAAMALVAAITSLPPLIGEIVAGKAIWPTQ
                     RGWLIVAYVAVLPSLLAQVWYIRGVAALGAARSGYIFYAIPVFASVMAVVFLGEPFGW
                     YHAAGLVLVLGGIAFAERFGIN"
     gene            complement(1337..1501)
                     /locus_tag="PLQ11_08695"
     CDS             complement(1337..1501)
                     /locus_tag="PLQ11_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25015.1"
                     /translation="MIGGIPARFVTDARIGGRHQLIRGAMTSYTREIIEILKQNGFEF
                     YRSGKGDHQI"
     gene            complement(1657..1887)
                     /locus_tag="PLQ11_08700"
     CDS             complement(1657..1887)
                     /locus_tag="PLQ11_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017487381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1902 domain-containing protein"
                     /protein_id="HRK25016.1"
                     /translation="MRRYAVVRAAWDDEVKVWYVEESDVPGLATEAPTLENLKQRVRD
                     IIPDLLEGQNDIPEELELEFIAYSHEKIRTAA"
     gene            complement(1960..3549)
                     /gene="serA"
                     /locus_tag="PLQ11_08705"
     CDS             complement(1960..3549)
                     /gene="serA"
                     /locus_tag="PLQ11_08705"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018042763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HRK25017.1"
                     /translation="MTKPRVLISDALSPAAVQIFKDRGIDVTFDASLGKDKDKLAAVI
                     GDYDGLAIRSATKATAKLLESAKNLKVIGRAGIGVDNVDIPAATAKGVIVMNTPFGNS
                     ITTAEHAIALMFALARQIPAADVSTQAGKWEKNRFMGVELEAKTLGVIGAGNIGSIVI
                     RKAIGLGMKVIAYDPFLTAERAKSMGIEKVELDGLLARADFITLHTPLTEKTRNILSR
                     EALAKAKKGVRIINCARGGLVDEEALADAIKAGHVAGAAFDVFSEEPATTNPLFGLEN
                     VVCTPHLGASTAEAQENVALQVAEQMSDYLLNGAISNAVNFPSIGAEEAPKLKPFVAL
                     AEKLGSFLGQLTEAPVKAIRIQYEGAVADLNVRPMTAAAVAGVMRAFMPEVNMVNAPV
                     AAKDKGIAIEEVKRAADSQFDSRIFINVIAEDMERHAAGTVFQDGKPRIVEIRHIAMD
                     AEFARHMLYVRNSDKPGFVGRFGTLLGDSGVNIATFNMGRNSPGGDAICLVAVDERVT
                     DDLLARIAAIPQVKRARPLEF"
     gene            complement(3670..4827)
                     /locus_tag="PLQ11_08710"
     CDS             complement(3670..4827)
                     /locus_tag="PLQ11_08710"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HRK25018.1"
                     /translation="MQKPAATPRVNTFSSGPCAKRPGWSLKALADAPLGRSHRAKSGK
                     AKLKLAIDLTRDVLDVPASYRIGIVPASDTGAVEMALWSMLGARGVDMLAWESFGEGW
                     VTDVVKQLKLSDVRTFTAPYGELPNLKKVDSKNRDVVFTWNGTTSGVRVPDAEWIADD
                     RQGLTICDATSAAFAQALDWTKLDVVTFSWQKVLGGEAAHGMLILSPRAVERLETYKP
                     AWPLPKIFRMTKGGKLIEGIFEGETINTPSMLCVEDYIDALQWAKKVGGLRGLIKRAD
                     ANTRALSAWVKKTEWIDFLAVKPKTRSNTSVCLKFVDPKVTALAADKQAAFAKAVVAI
                     LEKEGAGLDLGHYRDAPPGLRIWCGATVQTRDVKALIPWIEYAFATERAKL"
     gene            complement(4991..5884)
                     /locus_tag="PLQ11_08715"
     CDS             complement(4991..5884)
                     /locus_tag="PLQ11_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019903346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRK25019.1"
                     /translation="MGSLKTLSFAGAVAMVATSAALVTPAAAADLMPPPMVAPAPPPM
                     IDAGISGLYLRGDVGVGKLNHDGFHQPEVAAAPGGHWLRTSLGDTALMGVGVGYQVNS
                     FLRFDVTGEYRTAASLSGVDGYNFFCTFAGGSCPAIGTTIRRNNFWSGKMSSFVVLAN
                     AYADLGTWNGLTPFIGAGVGGAYNRIFGVTDFDPSDLGGGGYATDRSKWNFAWALHAG
                     LAYDVTSNVKVELAYRYLNLGNVATGGLNCLSPTGAVCGYGPLTVKSVSSSDVKLGVR
                     WLLADRAMPVAMPAGPLIRKY"
     gene            complement(6161..7504)
                     /gene="glmM"
                     /locus_tag="PLQ11_08720"
     CDS             complement(6161..7504)
                     /gene="glmM"
                     /locus_tag="PLQ11_08720"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020094009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HRK25020.1"
                     /translation="MGRKYFGTDGIRGRSNGIITPELALKVGQATGIIFRRGDYRHRV
                     VIGKDTRLSGYMIENALTAGFLSVGMDVMLLGPVPTPAVAMLTRSMRADLGVMISASH
                     NAFEDNGIKLFGPDGYKLKDDIEREIELLLDADLSRRLVDSKWIGRAKRVEGVHARYI
                     EFAKRTLPRNLSLEGLRVVIDCANGAAYSVAPDALWELGAEVVKLGVTPDGFNINREV
                     GSTAPATLQAKVREVRADIGIALDGDADRVLIVDEKGQIVDGDQLMAVVAKSFRDDGR
                     LAKPGVVATIMSNLGLERHLQDLGLSLARTAVGDRYVLEHMREHGFNVGGEQSGHIIL
                     SDFSTTGDGLVAALQVLNVVQREGRPVSEVCHRFEPLPQILMNVRYLGGQPLKEERVV
                     TAIEQARAKLGRNGRLIIRPSGTEPVIRVMGEGDDRDLVTEVVEGVVNVIAKAAA"
BASE COUNT         1422 a         2444 c         2322 g         1492 t
ORIGIN      
        1 gagccggtga tcaccactcc gtcataggaa cggatccggc tgagatctgg aataacgaag
       61 tcctcgtcgg ccggctggca gatatcggtc gccacatccg gcgccaggaa cctgatgcag
      121 ccggcataat tgtcgctggc ggtgcgacca agccgcgatt tttgccgggc ccgcccttca
      181 gcgtcgttgc cttccatgat cagaatgcgc aatgtcatcg tggcgctctg agttgctgac
      241 cgatccttgc cccttacggg aatgtttcga tcatggcaga ccgggttgca agaaccttta
      301 acgtgcaaac cccttccggc tgcgaatttt cggcgtgatc tcgagacttc tgaccaaccc
      361 cttcctgctg ttgacgctca cggcgctcgc ctgggccggc aacaccattg ccggccgcct
      421 cgccgtcggc gaggtctcgc ccatggtgct gacaaccctg cgctgggtga tctcgttcac
      481 gctgatgggc ctgttcgtac gcgccgacgt tgcacgggcc ctgccgttgt tccgccagcg
      541 gccgctttat ctcaccctca tgggcattat cggcttcacc ggcttcaacg cgatctatta
      601 cgtcgcggct cattacacca gcgccgccaa cctcggcatc atgcagagtt cgacgccgct
      661 gttcgtgatg atcggcgctg tcctgcttgg tcagagccag gtgaatttgg cccaggtgat
      721 cgggcttgcc atcgggcttg ccggcgttct catcgtggtc acgacagggc gctgggaggt
      781 cttgcacaac ctcgccttca atatcggcga tctgctgctg atcctgatca gcgcgatgta
      841 tgccggctat tcgctggggc tcaggcatcg cccgcagggt gtgccgggcc tgaccttctt
      901 tgccgccatg gcgctggtcg ccgccatcac atcgctgccg ccgctgatcg gtgaaatagt
      961 cgccggcaag gccatctggc cgacacagcg cggctggttg attgtcgcct atgtcgcggt
     1021 gcttccgtcg ctgctggcgc aggtctggta tatccgcggc gttgcggcgc tgggggcagc
     1081 ccgctcgggc tacattttct acgccattcc ggtattcgcc tcggttatgg cggtggtttt
     1141 cctcggcgag ccgttcggct ggtatcacgc cgccggcctg gtgctggtgc tcggcggcat
     1201 cgcctttgcg gagcggttcg ggatcaattg atcagaaggt ttttggcaaa ttcgcgtccc
     1261 gtaggatttt gttggccgta tgccttgaca ggactttcgc atcgacggtg acgcgcttgc
     1321 cacttacctt gtgtcgtcag atctgatgat caccttttcc ggagcggtaa aactcgaaac
     1381 cattctgctt caggatctcg atgatttcac gtgtgtagga agtcatggcg cctcgaatca
     1441 gttgatggcg cccaccaata cgcgcgtcag taacaaaacg cgccggaatt ccaccgatca
     1501 acagtaatcc acagtacgcc cgctgtgata gcgggcagca tttgctagat caggctgtat
     1561 tttgattgaa acataaaaaa cgaacaaact gatatttatc aataggttat agcatgtttt
     1621 tgtgattatc cggctgccac cgattagcag catttgttaa gcagcagtcc gaattttttc
     1681 gtgactatag gcaataaatt ccaattccag ctcttcgggg atgtcattct gaccttcaag
     1741 caaatcggga ataatgtcgc ggacgcgttg cttgaggttt tcaagggtcg gcgcttccgt
     1801 ggccaggccg ggcacgtcgc tttcctcgac ataccagacc ttcacctcat catcccaggc
     1861 agcgcgaaca acggcatatc ggcgcatcgg caaactcctg catgggtgcg gatcatcgcc
     1921 gatgacccgg tttccttcaa gtcacaactt ccaatcgcct cagaattcca gcggccgggc
     1981 gcgcttgacc tgcgggattg cggcgatcct ggcgagcagg tcatcggtga cccgctcgtc
     2041 gaccgccacc aggcagatgg catcgccgcc cggcgaattc cggcccatgt tgaaagtggc
     2101 gatattcacc cccgaatcgc ccagcaacgt gccgaaccgg ccgacaaagc ctggcttgtc
     2161 ggagttgcgc acatacagca tgtgccgggc gaactcggca tccatggcaa tatggcggat
     2221 ctcgacgatg cgcggcttgc cgtcctggaa caccgttccg gcggcatggc gctccatatc
     2281 ctcggcgatg acattgatga agatgcggct gtcgaactgg ctgtcggcgg cgcgcttgac
     2341 ctcctcgatg gcgatgccct tgtccttggc cgcgaccggc gcattgacca tgttcacctc
     2401 gggcatgaag gcgcgcatca cgcccgccac cgccgccgcc gtcatcgggc ggacattgag
     2461 atcggcaaca gcgccttcat actggatgcg gatggccttc accggcgcct cggtcagttg
     2521 gcctaggaac gagccgagct tttcggccag cgcaacgaac ggcttgagtt tcggcgcttc
     2581 ttcggcgccg atcgagggga agttcaccgc attcgaaatg gccccgttga gcagatagtc
     2641 gctcatctgc tcggccacct gcagcgcgac gttttcctga gcttctgcgg tcgaagcacc
     2701 gagatgcggc gtgcatacca cgttctcgag cccgaacagc ggattggtcg ttgccggctc
     2761 ctcggagaat acgtcaaagg ccgcaccggc cacatggccg gccttgatcg catcggccag
     2821 cgcctcctcg tcgaccaggc cgccgcgcgc gcaattgatg atgcgcacgc ccttcttggc
     2881 ttttgccagc gcctcgcgtg acaggatgtt gcgggtcttt tcggtcagcg gcgtatgcag
     2941 ggtgatgaaa tcggcccgcg ccagcaagcc atcgagttcg accttctcga ttcccatcga
     3001 cttggcccgt tcggcggtga ggaacggatc ataggcgatc accttcatgc cgaggccgat
     3061 cgccttgcgg atgacaatcg agccgatatt gccggcgccg atcacgccca gcgtcttggc
     3121 ttcgagttcg acacccatga accggttctt ctcccatttg ccggcctggg tggagacatc
     3181 ggccgccggg atctgacgtg ccagcgcgaa catcagcgca atcgcgtgtt cggccgtggt
     3241 aatcgaattg ccgaacggcg tattcatcac gatcacgccc ttggccgtgg cagccggaat
     3301 atcgacattg tcaacgccga tgccggcgcg accgatcacc ttgaggttct tggcactctc
     3361 gagcagtttc gctgtcgcct tggtggccga gcggatggcc agcccgtcat aatcgccgat
     3421 cacggcggcg agcttgtcct tgtccttgcc gagcgaggca tcgaaggtca cgtcaatgcc
     3481 gcgatccttg aagatctgca cggcggcggg cgacagggcg tcggaaatca gaacgcgggg
     3541 cttggtcatt ggaattcctc acgggagggg agaaacagaa ttcggcaata ggcagtcggc
     3601 aatcggggga ggcgcaaccg cgagcgcaag gtctcacgat tgcctgttac ggactgccga
     3661 ttgcctctct cagagcttgg cgcgctcggt ggcgaaggcg tattcgatcc acgggatcag
     3721 cgccttcaca tcgcgggtct gaacggttgc gccacaccag atccggaggc cgggaggcgc
     3781 atcgcgataa tggccgagat cgaggccggc gccttccttc tcgaggatcg cgacgaccgc
     3841 cttggcaaac gccgcctgct tgtctgccgc cagtgccgtc accttgggat cgacgaactt
     3901 caggcacacc gaggtattgg accgcgtttt gggcttgacc gcgagaaagt cgatccattc
     3961 ggtctttttc acccaggccg acagcgcgcg ggtattggca tcggcgcgct tgatcagccc
     4021 cctgaggcca ccgaccttct tggcccattg cagcgcgtcg atgtaatcct cgacacacag
     4081 catcgacggg gtgttgatcg tctcgccctc gaaaatgccc tcgatcagct tgccgccctt
     4141 ggtcatgcgg aaaatcttcg gcagcggcca ggccggcttg taggtctcga gccgctcgac
     4201 ggcgcgcggc gaaagaatca gcatgccgtg tgcggcttcc ccgcccagca ccttctgcca
     4261 ggagaaggtg acgacatcga gcttggtcca gtcgagcgcc tgggcaaaag ccgccgaggt
     4321 ggcgtcgcag atcgtcaggc cctgccggtc atcggcgatc cattcggcat cgggaactcg
     4381 cacgccggag gtggtgccgt tccaggtgaa caccacgtcg cggttcttgc tgtcgacctt
     4441 cttcaggttc ggcaattcgc catacggggc cgtgaaggtg cgcacatccg acagtttcag
     4501 ttgcttgacg acatccgtca cccagccttc accgaagctt tcccaggcca gcatgtcgac
     4561 gccgcgcgcg cccagcatcg accacagcgc catttcgacc gcaccggtat cggaagccgg
     4621 cacgatgcca atgcgatagc tcgccggcac gtccagcacg tcccgggtca ggtcgatggc
     4681 aagcttgagt ttggccttgc cggacttggc gcgatgcgag cggccaagcg gagcgtcagc
     4741 gagggctttg agggaccatc cggggcgctt ggcgcacggg ccggacgaaa aggtattcac
     4801 gcgaggggtc gcggcgggct tctgcatagc ttgtctccat ccttgcagat gggcgcgccc
     4861 cgttgggggg gcgtgtcccg ccgccgttcc taagcagaga gccctggccc gtcaagccac
     4921 cggacaacaa aaaggcgcgg ccatcggccg cgcccctgtc tccccggaag ggaaagcgat
     4981 caatatgcga tcaatacttg cggatgagcg gtccggcagg catggcaacc ggcatggcgc
     5041 ggtcggccag cagccagcgc acacccagct tcacatcgga actgctgacc gacttgaccg
     5101 tgagcgggcc atagccgcag acggcgccag tcgggctcag gcagttcagc ccgccagtcg
     5161 cgacattgcc cagattgagg tagcggtagg ccagctcgac cttgacgttg gaggtgacgt
     5221 cataggcaag accggcatgc agcgcccagg cgaagttcca cttggagcgg tcggtggcat
     5281 agccgccgcc gcccagatcg gacggatcga agtcggtcac gccgaagatg cggttgtagg
     5341 cgccgccgac acccgcgccg atgaacgggg tcaggccgtt ccaggtgcca aggtcggcat
     5401 aggcattggc cagcaccacg aagctcgaca tcttgccgga ccagaagttg ttgcggcgga
     5461 tggtcgtgcc gatcgccggg cagctgccac cggcgaaggt gcagaagaag ttgtagccgt
     5521 cgacaccact cagcgaggcg gcggtgcgat actcgccggt cacgtcaaag cgcaggaaac
     5581 tgttgacctg atagccgaca ccaacgccca tcagcgccgt atcgcccagc gaggtgcgca
     5641 gccagtgacc gccaggtgca gcggcaactt ccggctgatg gaagccgtcg tgattcaact
     5701 tgccgacacc gacatcgccg cgaagataga gccccgagat accagcatcg atcatcggcg
     5761 gcggcgcagg cgccaccatg ggcggcggca tcagatccgc tgcggcggca ggagtaacga
     5821 gagcggcaga cgttgcgacc atcgccacag cgcccgcgaa agacagggtt ttgaggctgc
     5881 ccatgagaaa tccctttggc tgcgcttcct ccgcgaagcc acgcgggaag caattccgac
     5941 acagcgaccg tcgcagctaa gggttaagac gctcttaacg ttaaaaatta accttaagaa
     6001 ttggcatcaa ccgcggctgt tgcgatgcaa tcctgcaaca ggggagattg aacgcgcgga
     6061 atacgctccg cgtgcggcag caaaaaacac acgatgctgt cagtgattcg gcgctcggtc
     6121 ctcatctggt gaacaagagg acgcccgggt ccgactgcag tcaggcggcc gccttggcaa
     6181 tcacattgac gacgccttcg accacttcgg tgaccagatc gcggtcgtct ccctcgccca
     6241 tcacgcgaat caccggctcg gttccggacg gacggatgat cagccggcca ttgcgcccca
     6301 gcttagcgcg ggcctgttcg atcgcggtga cgacccgctc ttccttgagc ggctgcccgc
     6361 cgaggtagcg gacattcatc aggatctgcg gcagcggctc aaaccggtgg cacacctcgc
     6421 tgaccggcct gccctcgcgc tgcaccacgt tgagcacctg caacgcggcc accagcccat
     6481 ccccggtcgt cgaaaagtcc gacaggatga tatgtcctga ctgttcgccg ccgacattga
     6541 acccatgttc ccgcatgtgt tcgagcacgt agcggtcgcc gaccgccgtg cgtgccaggc
     6601 tgagcccgag atcctgcagg tggcgctcca gcccgagatt ggacatgatg gttgccacaa
     6661 cgcccggctt ggcgagccgg ccatcgtcgc ggaagctctt ggccaccacc gccatcaatt
     6721 ggtcaccgtc gacgatctgc cccttctcat cgacgatcag cacgcggtcg gcatcgccat
     6781 caagcgcgat gccgatgtcg gcgcgcacct cgcgcacctt ggcctgcaag gtcgccggcg
     6841 cggtcgagcc cacctcgcgg ttgatgttga agccatccgg cgtcacacca agcttcacca
     6901 cttcggcacc gagttcccac agcgcatccg gcgccacaga ataggctgca ccattggcgc
     6961 agtcgatcac cacccgcagg ccctcgagcg acagattgcg cggcagcgtc cgcttggcga
     7021 actcgatgta gcgggcatgc acgccctcga cccgcttggc ccggccaatc catttcgaat
     7081 cgaccagcct ccggctcagg tcagcgtcca gaagcagttc gatctcgcgc tcgatatcgt
     7141 ccttgagctt gtaaccatct ggtccgaaca gcttgatgcc attgtcctcg aaggcattgt
     7201 gcgaggctga gatcatcacg ccgagatcgg cgcgcatcga gcgggttagc atcgccaccg
     7261 ccggcgtcgg cacggggcca agcagcatca catccatgcc gaccgacagg aagccggccg
     7321 tcagcgcgtt ctcgatcatg tagccggaca ggcgtgtatc cttgccgatc accacccggt
     7381 ggcgatagtc gccgcgccgg aaaatgatgc cggtggcctg cccgaccttc agcgccagtt
     7441 ccggagtgat gatgccgttc gaacgcccgc ggatgccgtc cgtgccgaaa tatttgcgac
     7501 ccatagagta ccctccgggc atgccggttg accaatcgga ttggttaaaa tcttaacgga
     7561 ttttttcgcg cggaaacagg cccgcgcagg cacggcacgg acaaatggtt aacgcagccg
     7621 gcagccgttc acgattgcgg ctgcggctcc atcccgccat cgggcgctga acggccacgg
//
LOCUS       DAOMXG010000117         7616 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_5035_length_7616_cov_9.440021, whole genome shotgun sequence.
ACCESSION   DAOMXG010000117 DAOMXG010000000
VERSION     DAOMXG010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 7616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7616
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5035_length_7616_cov_9.440021"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="PLQ11_08725"
     CDS             complement(<1..692)
                     /locus_tag="PLQ11_08725"
                     /inference="COORDINATES: protein motif:HMM:NF022738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3095 family protein"
                     /protein_id="HRK25021.1"
                     /translation="MPKRFATISEVPLSDRFYHDLPKATDLVDLVGDPFFQPLPGDWH
                     IGLAELAPAEGPVAPAEARARAKAVMAPLAAFMNAVESMTLPLGFGGHWSWFAVSAHN
                     RDIAGEALARSAGLVRDDFGLDLRVALVPVAEVRAAGHDVRIARHVRSPAVSYAMARG
                     GGLKWARSQAAAGRFIVGPAPEGLRPDLADLSARFAFVPARHGLILSLAVTPQRAETP
                     GFAGLLGDLLAMT"
     gene            complement(1068..2087)
                     /gene="ilvC"
                     /locus_tag="PLQ11_08730"
     CDS             complement(1068..2087)
                     /gene="ilvC"
                     /locus_tag="PLQ11_08730"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019443468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HRK25022.1"
                     /translation="MRVYYDRDADLNLIKGKKVCIVGYGSQGRAHALNLKDSGAKEIV
                     IALKPGSATAKKVEADGLKVKSVADAAKWADVMMMATPDELQADIWREEIAPNIRDGA
                     AIAFAHGLNVHFGLIEPKKTIDVIMIAPKGPGHTVRSEYQRGGGVPCLIAIHQDASGN
                     AHDIALSYACGVGGGRSGVIETNFKEECETDLFGEQVVLCGGLVELIRAGYETLVEAG
                     YAPEMAYFECLHEVKLIVDLIYEGGIANMNYSISNTAEWGEYVTGPRIITAETKAEMK
                     RVLKDIQTGKFTSEWMQEWKSGAARFKGIRRVNDAHDIEKVGEKLRGMMPWIAKNKLV
                     DKARN"
     gene            complement(2197..2814)
                     /locus_tag="PLQ11_08735"
     CDS             complement(2197..2814)
                     /locus_tag="PLQ11_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018324334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HRK25023.1"
                     /translation="MEGFSSSTLAALFLFAFSSSFTPGPNNMMLLASGVNFGVRRSIP
                     HMAGVDLGFTLMVFLIGIGLGTIFTRFPPLYLALKILGIGYLLWLAWRIAHSGPIREG
                     ESRGRPMTFLEACAFQWVNPKAWMMGVTAIATYRVFADSTVNAIFVALNFAVANLPSV
                     LVWVWFGAGMRRLLSDPERVRMFNWTMAALLVASLWPVLAEFLPV"
     gene            complement(2820..3365)
                     /gene="ilvN"
                     /locus_tag="PLQ11_08740"
     CDS             complement(2820..3365)
                     /gene="ilvN"
                     /locus_tag="PLQ11_08740"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003611278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HRK25024.1"
                     /translation="MLSPYSDPTYRITQERRTLAIIVDNEPGVLARIAGMFSGRGYNI
                     ESLTVSETEHEKHRSRITVVTRGTPQVIDQIKAQLDRLVPVHRVVDLTLSGANIEREL
                     ALVKVRQEGETRSEHRMEALRLATAFGARPIDATLNSFIFEIAGSSDEVERFIGLMAH
                     VGLVEVARTGAAAISRGPEAL"
     gene            complement(3367..3738)
                     /locus_tag="PLQ11_08745"
     CDS             complement(3367..3738)
                     /locus_tag="PLQ11_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HRK25025.1"
                     /translation="MVREARTFARRQRQHPTAAEDELWQRLRDRRFLGLKFKRQEPIG
                     PYTVDFLRFERKLVIELDGRHHGEQKHYDTMRTKELENQGFFVIRFANAAVPGDIDAV
                     FQTLKETLTPGPSPKLERGEF"
     gene            complement(3782..5548)
                     /locus_tag="PLQ11_08750"
     CDS             complement(3782..5548)
                     /locus_tag="PLQ11_08750"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018262911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HRK25026.1"
                     /translation="MTMSETMTGAEMVIRALQDQGVEHIFGYPGGAVLPIYDAIFQQD
                     KVKHILVRHEQGAVHAAEGYARSSGKVGCVLVTSGPGATNAVTGLTDALLDSIPIVCF
                     TGQVPTHLIGSDAFQECDTVGITRHCTKHNYLVKRIEDLPRVIHEAFTVASSGRPGPV
                     VIDIPKDVQFAKGRYARPTNSEHRTYRPKVDGDIGQIERAIELMSKAKRPVFYTGGGI
                     INAGPAAAASLRELVRATGYPITSTLMGLGAYPASDPQWLGMLGMHGTYEANLAMHDC
                     DVMICIGARFDDRITGRLDAFSPKSKKIHVDIDPSSINKNVKVDVPIIGDAGRVLSEM
                     LRLWKEKKPRLETAAHKDWWAQISRWRARNCLAYKRSDSEIKPQYAVERLYALTKARN
                     SYITTEVGQHQMWAAQFMHFEEPNRWMTSGGLGTMGYGLPAAVGVQMAHPEALVVCVA
                     GEASVLMNMQEMSTAAQYRLPVKIFILNNEYMGMVRQWQELLHGGRYSNSYSEALPDF
                     VKLAEAYHAKGIRCSDPKELDAKIQEMIDYPGPVIFDCLVTKEENCFPMIPSGKAHNE
                     MIMPDFEGDTGEIIDAKGKQLV"
     gene            5887..7299
                     /locus_tag="PLQ11_08755"
     CDS             5887..7299
                     /locus_tag="PLQ11_08755"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRK25027.1"
                     /translation="MGKSFLFVANIDDQLWAPSDRALLALWRREVAGPQPPHIRLIDS
                     AIMRELGDYLTVAERSQFGEFSFLFCGEAVADQLGRNLSSRSTSDLPPKSAAQFRRNF
                     NQAIELMAPTFTIDRAADCGHVHLWGRLHLPFRESNGAVLIVTLHRPREYTDELLREI
                     LDAAADGILAVRAKRDARGRIEDFEVIAANIPMAELLATDRERLAGETLLDLLPAVRE
                     DGAWIRHATVVDTRLGATFESCYDIHGTKRWFRIVSAPLNDGLVISFTDITELKLLTL
                     RLQEQQQQLEAEIARRLKVEDELWRLAHLDALTGIANRRAMHVRATKCLADAALRGQT
                     CAMIVLDIDHFKLINDTHGHAAGDATIVRVAEIARSGLRGDNDIVARMGGEEFAMLLN
                     DSNIEAGLAVAERLRRDIEQARISFGGKDISFTVSFGVAASRDGCTYEQLLQSADNAL
                     YRAKRSGRNQAQAEDVPTAA"
     gene            complement(7303..>7616)
                     /locus_tag="PLQ11_08760"
     CDS             complement(7303..>7616)
                     /locus_tag="PLQ11_08760"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA dimethylallyltransferase"
                     /protein_id="HRK25028.1"
                     /translation="GSWLGLFLDPQRDMLFRRIDRRFETMIDAGALDEVRALAGLGLP
                     ANRGVMKAHGVPHLLGYLRGELPLAEAIMLGQRDTRRYAKRQLTWARRFMADWQWLTP
                     P"
BASE COUNT         1429 a         2495 c         2330 g         1362 t
ORIGIN      
        1 gtcatcgcaa gaagatcgcc caacagcccg gcaaagccgg gcgtttcagc ccgctgcggc
       61 gtgacagcaa gcgacaggat gaggccatgc cgcgccggca caaaggcaaa ccgtgccgac
      121 agatcggcaa gatccggacg cagcccctcc ggcgccggac caacgataaa ccgaccggcc
      181 gccgcctgtg atcgcgccca tttgaggccg ccgccgcgcg ccatcgcata gctgaccgcc
      241 ggcgaccgca catgccgggc gatccggaca tcatgcccgg ccgcgcggac ctcggcgacc
      301 ggaaccagcg ccacccggag atccagaccg aaatcgtcgc gcaccagtcc ggccgaccgc
      361 gccagcgcct cgccggcaat gtcccggttg tgggcactga ccgcgaacca cgaccagtga
      421 ccgccaaagc ccagcggcag ggtcatggat tcgacggcat tcatgaacgc cgccagcggc
      481 gccatcaccg ccttggcccg ggccctggcc tcagcgggcg ctaccggccc ttcggccggg
      541 gccagttcgg caaggccgat atgccaatcg ccgggaagtg gctggaagaa ggggtcgccg
      601 acaaggtcca ccagatcggt ggcctttggc aggtcgtgat agaaccggtc ggacaagggg
      661 acctcggaga tggttgcgaa cctctttggc attttccgcg cgcacaaaaa agccccggcg
      721 ttctccggcc cggaccgacc ctcgccccgc ccctcacccc aaccctctcc ccgcaagcgg
      781 ggagaggggg cgcaagcggg cgcggagaca ggttccccct ccccgctggg gacggaagaa
      841 gcgcaaggca acaggaacag gccccctctc cccgctcgcg gggagagggc tggggtgagg
      901 ggcatcggtg aggggaatcg cagaaaaagc cggtctgacg accggctctt cgcaatcctc
      961 agcaccgcgc tgcaagcgtc agttgcgcgc cttgtcgacg agcttgttcc tggcgatccc
     1021 gaaagggcgg catgcttgcc gcccgtccag agctatagag ccgtggatca gttgcgcgcc
     1081 ttgtcgacga gcttgttctt ggcgatccac ggcatcatgc cgcgcagctt ctcgccgacc
     1141 ttctcgatgt catgcgcatc gttgacgcgg cggataccct tgaagcgggc ggcgcccgac
     1201 ttccattcct gcatccattc ggaggtgaac ttgccggtct ggatgtcttt caggacgcgc
     1261 ttcatctcgg ccttggtctc ggcggtgatg atgcgcggtc cggtgacata ctcgccccac
     1321 tcggcggtgt tggagatcga gtagttcatg ttggcaatgc cgccctcgta gatgaggtcg
     1381 acgatcagct tcacctcatg caggcactcg aaataggcca tttccggcgc gtagcctgcc
     1441 tcgaccagcg tctcatagcc ggcgcggatc agttcgacca gaccgccgca cagcacgacc
     1501 tgctcgccga acaggtcggt ttcgcattct tccttgaaat tcgtctcgat gacgcccgaa
     1561 cggccgccgc cgacgccgca ggcgtaggaa agcgcgatgt catgggcatt gccggaagcg
     1621 tcctgatgga tggcgatcag gcagggcacg ccgccgccgc gctgatattc ggagcgcacg
     1681 gtgtggcccg ggcccttcgg cgcgatcatg atcacgtcga tggtcttttt cggctcgatc
     1741 aggccgaaat gcacgttgag gccatgggca aaggcgatcg ccgcgccgtc acggatgttg
     1801 ggagcgatct cctcgcgcca gatgtcggcc tgaagctcgt ccggggtcgc catcatcatc
     1861 acatcggccc atttcgccgc atcggccacg gacttgacct tgaggccatc cgcttccacc
     1921 ttcttggcag tggcggagcc cggcttgagc gcgatcacga tctccttggc gccggaatcc
     1981 ttgaggttca gcgcatgggc gcggccctgc gagccatagc cgacgatgca gacctttttg
     2041 cccttgatga gattgagatc ggcgtcgcga tcataataaa cgcgcatgag gaagtgctcc
     2101 ttggtcgtgg gatccatgta gggaatttgc gggcgcttaa agacctaaga cagcgcagcg
     2161 tcaagcgcca tcacgggaca gggcctgctc catggttcaa acaggcagga attcggccag
     2221 aaccggccag agcgaagcca ccagcagcgc cgccatggtc cagttgaaca tccgcacacg
     2281 ctcgggatcg ctgagcagcc gccgcatgcc ggcaccgaac cagacccaca ccaaaacgct
     2341 tggcagattg gcaacggcga agttcagcgc aacgaaaatg gcgttgaccg tcgagtcggc
     2401 gaacacccga taggtagcaa tcgccgtaac ccccatcatc caggccttcg gattgaccca
     2461 ctggaaggcg caggcctcaa gaaaggtcat cggccggcca cggctctccc cctcccggat
     2521 cggacctgaa tgggcgatcc gccaggccag ccacagcaga tagccgatgc cgaggatctt
     2581 cagcgccaga tacagtggcg ggaaccgcgt gaagatcgtc cctagcccga tgccgatcag
     2641 gaacaccatc agcgtgaagc cgagatcgac ccccgccata tgcgggatcg accggcgtac
     2701 accgaaattc acgccggaag ccagcagcat catgttgtta ggccccggcg tgaacgacga
     2761 cgagaacgcg aacaggaaca gcgcggccag tgtcgaggag gaaaagcctt ccactgcgcc
     2821 tacagggcct ctggaccacg gctgatcgcc gccgcaccgg tccgcgccac ctcgaccagg
     2881 ccgacatgcg ccatcagccc gatgaagcgc tccacctcgt cggatgagcc ggcgatctcg
     2941 aagatgaagc tgttgagcgt cgcatcgatc ggcctggcgc cgaaggcggt ggccagccgc
     3001 agcgcctcca tgcggtgttc ggagcgcgtc tcgccctcct gccgcacttt gaccagcgcc
     3061 agttcccgct cgatgttggc gccggacagc gtgagatcga cgacgcgatg caccggcacc
     3121 agccggtcga gctgcgcctt gatctggtcg atcacctgag gcgtgccgcg ggtgaccacc
     3181 gtgatgcgcg agcggtgctt ttcgtgctcg gtctcggaaa cagtgagact ctcgatgttg
     3241 tagccgcgac cggaaaacat gccggcgatg cgcgccagaa cgcccggctc gttgtcgacg
     3301 atgatggcga gcgtgcgccg ctcctgggtg atgcgatagg tggggtcgga atagggagag
     3361 agcatcttag aactcccccc tctccagctt gggagagggg ccgggggtga gggtttcttt
     3421 cagggtttgg aacacggcgt cgatgtcgcc cggaacagcg gcattggcga aacggatgac
     3481 aaaaaagcct tggttctcca gctctttggt tcgcatggta tcgtagtgct tctgctcgcc
     3541 atggtgcctg ccatcgagtt caataaccag tttccgttca aagcggagga agtcgacggt
     3601 gtatggaccg atcggctcct gccgcttgaa cttcagtccg aggaagcgac ggtcacgcag
     3661 ccgttgccag agttcgtctt cggcggcggt tggatgttgc cgctgcctgc gtgcaaaggt
     3721 gcgggcttct cgaaccatga accctgaccc ccgcccctct cccaccggga gaggggatca
     3781 atcaaaccag ctgcttgccc ttggcgtcga tgatctcgcc ggtatcccct tcgaaatccg
     3841 gcatgatcat ctcgttatgt gccttgcccg aaggaatcat cgggaagcag ttctcttcct
     3901 tggtgacgag gcaatcgaag attaccgggc cgggatagtc gatcatctcc tggattttcg
     3961 cgtccagttc cttcggatcc gaacagcgga tgcccttggc gtgataggct tccgccagct
     4021 tgacgaaatc gggcaacgct tccgaatacg agttggaata gcgcccgccg tgcagcagtt
     4081 cctgccactg gcgcaccatg cccatgtatt cgttgttgag gatgaagatc ttcaccggca
     4141 ggcgatactg cgccgccgtc gacatctcct gcatgttcat cagcaccgag gcttcacccg
     4201 ccacacagac gaccagcgct tcgggatggg ccatctgcac gcccaccgcc gccggcaggc
     4261 catagcccat cgtgccaagc ccgcccgacg tcatccagcg gttgggctct tcgaaatgca
     4321 tgaactgcgc cgcccacatc tgatgctggc cgacctcggt ggtgatatag ctgttgcgcg
     4381 ccttggtcag agcataaagc cgctcgaccg catattgcgg cttgatctcg ctgtccgagc
     4441 gcttgtaggc gaggcagttg cgcgcccgcc acctagaaat ctgcgcccac caatccttgt
     4501 gcgctgcggt ctccagcctg ggctttttct ccttccacag gcgcagcatc tcgctcagca
     4561 cccggccggc atcgccgatg atcggcacat cgaccttgac gttcttgttg atcgacgaag
     4621 ggtcgatatc gacatggatc ttcttcgatt tcggcgagaa ggcatcgagg cgcccggtaa
     4681 tccggtcatc gaatcgcgcg ccgatgcaga tcatcacatc gcaatcgtgc atcgcgaggt
     4741 tggcttcata ggtgccgtgc atgccgagca tgccgagcca ttgcggatcg gaggccggat
     4801 aggcgcctag ccccatcagc gtcgaggtga tgggatagcc ggtcgcgcgc accagttcgc
     4861 gcaggctcgc cgccgccgcc gggccggcgt tgatgatgcc gccgccggta tagaacaccg
     4921 gacgcttggc cttgctcatc agctcgatgg cgcgttcgat ctggccgata tcgccatcga
     4981 ccttcggccg gtaggtgcgg tgctccgaat tggtcggccg cgcatacctg cccttggcga
     5041 actggacatc cttcgggatg tcgatgacca ccgggccagg ccggccgctg gaggccaccg
     5101 tgaacgcctc atggatgacg cgcggcaggt cctcgatgcg cttgaccaga taattgtgct
     5161 tggtgcagtg ccgggtaatg ccgacggtgt cgcattcctg aaaggcatcc gagccgatca
     5221 gatgcgtcgg cacctggccg gtgaagcaga cgatcggaat ggaatcgagc agcgcgtcgg
     5281 tcagtccggt caccgcattg gtcgcgccgg gaccggacgt caccagcacg cagccgacct
     5341 tgccggacga gcgcgcatag ccctccgcgg catgcaccgc gccctgctca tggcgaacca
     5401 ggatgtgctt gaccttgtcc tgctggaaga tcgcgtcata gatcggcaga accgcaccgc
     5461 ccggataacc gaagatgtgc tccaccccct gatcctgcag cgcgcggatc accatttccg
     5521 cgccggtcat cgtctcgctc atcgtcatac tcctgaacct tggtcggctc tttggcgggt
     5581 ttgtcggaaa aaggcaaaaa gaaaaggccc ctttcgggac cttgcggtta cgcgccggcg
     5641 cttgggatcg tcgggtcaga cccgtcccac ctccggcgcg cagcatacaa taagcgtgaa
     5701 ggacatcgat ccccctcctg ttcggggcaa cattaggagc agctgaagcg ccaagtcaag
     5761 gccgggccgc attgcacctt ggttcactga aggcgtgggc agtgccggcc cgttgtgcag
     5821 cccggtgcag agagaagttt gtcaacctgt cataaacttc attggtgata tacaatcaga
     5881 acgtgtatgg ggaagtcgtt cttgttcgtc gccaacattg atgaccagct ttgggctccg
     5941 tccgatcggg cgctgctggc gctttggcgc agggaggtgg ctggcccgca accgccgcat
     6001 atccgcctga tcgacagcgc gatcatgcgt gaattgggcg actatctcac ggtcgccgaa
     6061 cgcagccagt tcggcgagtt cagcttcctc ttctgcggcg aggccgtcgc cgaccagctg
     6121 gggcgcaacc tgtcatcgcg ttcgaccagc gatctgccgc ccaaatcggc agcgcagttc
     6181 cggcgcaact tcaaccaggc catcgaactg atggccccga cctttaccat cgatcgtgcg
     6241 gccgattgcg gccatgtcca tctgtggggg cggctgcatc tgccatttcg cgaatccaac
     6301 ggcgccgtgc tgatcgtcac cctgcaccgc ccgcgcgaat acaccgacga actcctgcgc
     6361 gagattctcg atgctgccgc cgacggcatt ctggcggtcc gcgccaagcg cgatgcgcgt
     6421 ggcaggatcg aggatttcga ggtcatcgcc gccaacatcc cgatggcaga actgctggcg
     6481 accgatcgcg agcgtctggc tggcgagact ttgctcgatc tgctgcccgc tgtgcgtgag
     6541 gatggcgcct ggatccgcca cgccacggtt gtcgatacgc ggctcggcgc cacattcgaa
     6601 agctgctatg atatccacgg aaccaagcgc tggttccgca tcgtctccgc cccgctcaac
     6661 gacggtctgg tcatcagctt caccgatatc accgagttga aactcctgac cctgcgcctt
     6721 caggagcagc agcagcagct cgaagccgag atcgcccgtc gtctgaaagt ggaagacgag
     6781 ttgtggcggc tcgcccatct cgatgccctc accggcattg ccaaccgtcg cgccatgcat
     6841 gtccgcgcaa ccaaatgcct ggcagatgcg gctctgcgcg gccagacctg cgccatgatc
     6901 gtgctcgaca tcgaccattt caagctgatc aacgataccc acggccatgc cgccggcgat
     6961 gcaaccatcg tccgggtggc cgagatcgcc cgctccggcc tgcgcggcga caatgatatc
     7021 gttgcccgca tgggcggcga ggaattcgcc atgcttctca acgattccaa catcgaagcg
     7081 ggtcttgccg tcgccgaacg cctgcggcgc gacatcgaac aggcccgcat cagcttcggc
     7141 gggaaagaca tcagcttcac cgtgagcttc ggcgtggccg ccagcaggga cggctgcacc
     7201 tacgaacagc ttctgcaaag tgcagacaat gcgctctacc gcgcaaagcg ttccggccgg
     7261 aaccaggcac aggcagagga cgttccgacc gctgcctgac tttcagggcg gcgtcagcca
     7321 ctgccagtca gccatgaacc ggcgtgccca ggtcagctgc cgcttggcgt agcggcgcgt
     7381 atcgcgctgg cccagcatga tcgcctcggc caatggcagt tcgcccctga ggtaaccgag
     7441 cagatgcggc acgccatgcg ccttcatcac accccggttg gccggaagcc cgagccctgc
     7501 cagcgcccgc acctcatcaa gcgcaccggc gtcgatcatc gtctcgaacc gtcggtcgat
     7561 gcgccggaac agcatgtccc gctgtggatc gagaaacagc cccagccatg agccag
//
LOCUS       DAOMXG010000118         7552 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_5089_length_7552_cov_8.461518, whole genome shotgun sequence.
ACCESSION   DAOMXG010000118 DAOMXG010000000
VERSION     DAOMXG010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 7552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7552
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5089_length_7552_cov_8.461518"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="PLQ11_08765"
     CDS             <1..584
                     /locus_tag="PLQ11_08765"
                     /inference="COORDINATES: protein motif:HMM:NF028235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HRK25029.1"
                     /translation="GAAMSRAGQGRARLLAAAKELLWERGYEHMSPRDVLDRSGVGQG
                     SLYHHFSGKLELARAALGEMVTEETAAMDAIFAADKTPLRRLEEYLARKRAALRGCRI
                     GRLANETIMEVPEFRTFVATYLDHVEGLIRKAVAEAQGAGMLAAEADPDALAALFLAV
                     IEGGFVLARAHWDPSRMERAIRGSEAALALMKR"
     gene            complement(638..1417)
                     /locus_tag="PLQ11_08770"
     CDS             complement(638..1417)
                     /locus_tag="PLQ11_08770"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HRK25030.1"
                     /translation="MAEFALPKNSQLVPGKTWPKPAGASRLTEFHIYRWDPDTGANPR
                     MDTYFVDRDDCGPMVLDAILWIKNKVDPGLTFRRSCREGICGSCAMNIGGINTLACTK
                     AMDDIAGVIKIYPLPHMPVVKDLVPDLTRFYAQHASIEPWLKTETPAPPTEWRQSRED
                     REKLDGLYECILCACCSTSCPSYWWNGDRYLGPAILLQAYRWLIDSRDEATGERLDNL
                     EDPFRLYRCHTILNCSKTCPKGLNPAKAIAEIKKMMVARQF"
     gene            complement(1421..3244)
                     /gene="sdhA"
                     /locus_tag="PLQ11_08775"
     CDS             complement(1421..3244)
                     /gene="sdhA"
                     /locus_tag="PLQ11_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007199320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HRK25031.1"
                     /translation="MAKSASPAVNGRAYPLVDHAYDVVVVGAGGAGLRATLGMAEQGL
                     RTACITKVFPTRSHTVAAQGGIAASLANMGPDCWQWHMFDTVKGSDWLGDNDAMEYLA
                     REAPAAVYELEHYGVPFSRTEEGKIYQRPFGGHTTEYGEGPPVQRTCAAADRTGHAIL
                     HTLYGQSLRNNAEFFVEYFALDLIMKDGECQGVIAWKLDDGTLHRFRAKLVVLATGGY
                     GRAYFSATSAHTCTGDGGGMVARAGLPLQDMEFVQFHPTGIYGSGCLITEGARGEGGY
                     LTNSAGERFMERYAPTYKDLAPRDFVSRCMTMEIREGRGVGRQNDHIYLHLDHLDPKI
                     LHERLPGISESAKIFAGVDLTREPIPVIPTVHYNMGGVPTNYWGQVVNPTKSDEDAIV
                     PGLMAVGEAGCASVHGANRLGSNSLIDLVVFGRAASIRAGQVLDRKAPIPSINEAEDD
                     KILARFDRMRNANGATPTADLRLTMQRAMQEDVAVFRDGPTLKSGCKRLSDVWAAKSD
                     IKVTDRSLVWNSDLVETLEFENLMANAITTVYSAEARKESRGAHAREDFTARDDKTWR
                     KHTLSYLSEDGKVRLAYRSVHTKTLTGEMSEEKIAPKVRKF"
     gene            complement(3247..3642)
                     /gene="sdhD"
                     /locus_tag="PLQ11_08780"
     CDS             complement(3247..3642)
                     /gene="sdhD"
                     /locus_tag="PLQ11_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HRK25032.1"
                     /translation="MKNSSSSMATPRSRVKGLGAAKTGTGHFWAQRITGVALIPLVLI
                     FIITVIRLAGADHAGAVAILKGPLTAILMLAFVLTGVYHMRVGMQVIIEDYVHGEGVK
                     LVCLMLNTFFAALVGLASVYALLRISFGL"
     gene            complement(3655..4053)
                     /gene="sdhC"
                     /locus_tag="PLQ11_08785"
     CDS             complement(3655..4053)
                     /gene="sdhC"
                     /locus_tag="PLQ11_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008548214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HRK25033.1"
                     /translation="MADAKTPAERPLSPHLQIYKPTLTMMMSIVHRITGAALYFGTLL
                     LVGFLVATAAGKDAHAMVGAIYGSFLGKLVLFGYTWALIHHMLGGIRHFIWDTGAGLG
                     HPERETIVKATAVGSVALTLALWLIFGFGK"
     gene            complement(4179..4493)
                     /locus_tag="PLQ11_08790"
     CDS             complement(4179..4493)
                     /locus_tag="PLQ11_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein"
                     /protein_id="HRK25034.1"
                     /translation="MSTPPFTAGSDHVIVRIRLTPNAHDSRIDGLRTLSDGSTVLAVR
                     VRAIPEDGAANAALERLLAETAGVPRSAVQLIGGPAQRLKTIRIDGDPDTLRQRLVAA
                     TG"
     gene            complement(4490..4786)
                     /locus_tag="PLQ11_08795"
     CDS             complement(4490..4786)
                     /locus_tag="PLQ11_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HRK25035.1"
                     /translation="MRAFLWLFDTVISIYIWLVIGQIVMSWLIQFGIVNARQPFVMQL
                     SEFLYRVTEPVLGPIRRRLPDLGGIDISPLILLVGLYFIQILVHDMVSSLARAL"
     gene            4975..5050
                     /locus_tag="PLQ11_08800"
     tRNA            4975..5050
                     /locus_tag="PLQ11_08800"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5008..5010,aa:Ala,seq:cgc)
     gene            complement(5109..6194)
                     /locus_tag="PLQ11_08805"
     CDS             complement(5109..6194)
                     /locus_tag="PLQ11_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25036.1"
                     /translation="MPFSSSARCIRVTREDLAGVIDDCGACGPLSPRRLMGAAHVARP
                     GATDGLRDAAGVLSNPRSRITLRLWSDEAACAETTILFPGDPATGGGYTLNDHAGECE
                     VCGPVEAHDLLRLIAPLLPPAPAAPPFVLQLDVVAMATLAVLVDETSAVIRSRRVARA
                     LDGATLADSALADASPLTTEHLLSALTNWWGLSRFDQLLTYVVALGGRPEAPAAAEVA
                     EAVQRLQAAGLVVVGDGQRLMPAAMLEPLSAAALGLVAGAQWQRAALTGEGVLALDER
                     ILLAGTGGTLFEVTLTAAGLVRLARTDRHSVTAYLVGELSGFGKPAPDPVAPVKAAQA
                     PRRFCHQCGKPTGETDRFCAHCGEKLM"
     gene            complement(6204..>7552)
                     /locus_tag="PLQ11_08810"
     CDS             complement(6204..>7552)
                     /locus_tag="PLQ11_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25037.1"
                     /translation="GQGLGRLGSTVAREGAQVALGGVMGAAGRATTDGNVTEGFLDGL
                     ASGVGSSVGGHAAKAGVARAEPLPLPGSETFRPVQTDPKLTAADIQLDADAAVRKTQA
                     QGVVDDLKRAIASGTPDEQKEALKRVLENRDAKLLMKGAEVDAATKTAFADLTEQHRT
                     KPVFAATADHLNQQKGTDGAQRFVVREPDPANPGGFLERKVLPTDFRSTSGTAGKAPG
                     MDLDMTANATIIDRTTGRPARAADLDAAVGRACTRLGISKSGQEINLMHGESLERYTL
                     RPGEDPAKFIPRVASGNVTGAEGRSLAEVTGAKLAEADVLHTGAGAVSEKCRIAAKDF
                     DRITRPLMEAKSGSRLPPVFAVRDAVTGETPLDIMRAVANNDMPPGTGNARFRALTGM
                     DLSAGADKLAQMPEFVAKFGATGSGAAAPDGPAPLLPAGEAARAALRQALRLGSDR"
BASE COUNT         1296 a         2389 c         2539 g         1328 t
ORIGIN      
        1 ccggggccgc aatgagccgc gccggacagg gccgcgcccg gctgctcgcc gcagccaagg
       61 agctattgtg ggagcgcggt tacgagcata tgagcccgcg cgatgtgctt gatcgctcag
      121 gcgtgggaca gggcagcctc tatcaccatt tttccggcaa gctcgagctt gcccgcgccg
      181 ctcttggcga gatggtcacc gaggagacgg cggcgatgga tgcgattttc gctgccgaca
      241 agacgccgct gcgtcgcctg gaggagtatc ttgcgcgcaa gcgcgcagcg ttgcgcggct
      301 gccggatcgg ccggctggca aatgaaacca tcatggaagt gccggaattc cggacgttcg
      361 tcgcgaccta tctcgatcat gtcgaggggc tgatccgcaa agccgtcgcg gaggcgcaag
      421 gggcggggat gctcgcggcc gaggccgatc ccgacgccct ggcggcgctg tttcttgcgg
      481 tcattgaagg cggtttcgtg ctcgcccgcg ctcattggga cccaagccgg atggagcggg
      541 caatccgcgg ctcggaagca gcgctggcgc tgatgaaacg gtaatgtgcc cataaaaaag
      601 gcgccgaagg cgcctttttc tgccgaacag cgcggtatca gaactgtcgc gccaccatca
      661 tcttcttgat ttcggcgatg gccttggccg ggttcagccc cttcgggcag gtcttggagc
      721 agttcaggat ggtgtggcag cgatagagcc ggaacgggtc ctcgagattg tcgagccgct
      781 cgccggtcgc ctcgtcgcgg ctgtcgatca gccagcgata ggcctgcagc aggatcgccg
      841 ggccgaggta gcggtcgccg ttccaccagt agctcgggca ggaggtggag cagcaggcgc
      901 agaggatgca ctcgtagagg ccatccagct tctcgcgatc ctcacggctc tggcgccatt
      961 cggtcggcgg tgccggcgtc tcggtcttga gccagggttc gatcgaggcg tgctgggcgt
     1021 agaagcgggt gaggtccggc acaagatctt tcaccaccgg catgtgcggc agcgggtaga
     1081 tcttgatgac gccggcgatg tcatccatcg ccttggtgca ggcgagggtg ttgatgccgc
     1141 cgatgttcat ggcgcaggag ccgcagatgc cttcgcggca ggagcgacgg aaggtcagcc
     1201 ccggatcgac cttgttcttg atccacagaa tggcatccag caccatcggt ccgcaatcgt
     1261 cgcgatcgac aaaataggtg tccatgcgcg gattggcgcc ggtatcggga tcccagcggt
     1321 agatgtggaa ttcggtcagc ctgctggctc cggccggctt cggccaggtc ttgccgggga
     1381 caagctgcga gttcttggga agcgcgaatt cggccatggc tcagaacttc ctcaccttcg
     1441 gggcgatctt ttcctcggac atctcgccgg tcagcgtctt ggtatgcacc gagcggtagg
     1501 cgaggcggac cttgccatcc tcggagaggt aggacagcgt gtgcttgcgc caggtcttgt
     1561 cgtcgcgggc ggtgaagtcc tcgcgggcat gagcgccgcg gctctccttg cgggcctcgg
     1621 ccgaatagac cgtggtgatg gcgttggcca tcaggttctc gaactccagg gtctcgacga
     1681 ggtcggagtt ccagaccagc gagcggtcgg tcaccttgat gtcggacttg gcggcccaga
     1741 cgtcggacag gcgcttgcag ccgctcttga gcgtcggccc atcgcggaac accgcgacat
     1801 cctcttgcat ggcgcgctgc attgtcaggc gcaggtcggc ggtcggcgtc gcgccattgg
     1861 cgttacgcat ccggtcgaac cgggcgagaa tcttgtcgtc ctcggcctcg ttgatcgagg
     1921 ggatcggcgc cttgcggtca agcacctgtc cggcgcggat cgaggcagcg cggccgaaca
     1981 cgacgaggtc gatcagcgag ttggagccga ggcggttggc gccatgcacc gaggcgcagc
     2041 cggcctcgcc cactgccatc aggccgggga cgatcgcgtc ctcgtccgac ttggtcgggt
     2101 tgaccacctg gccccagtaa ttggtcggca cgccgcccat gttgtaatgc acggtcggga
     2161 tcaccgggat cggctcgcgg gtgagatcga caccggcgaa gatcttggcg ctttccgaaa
     2221 tgccgggcag gcgctcgtgc aggatctttg ggtcgagatg gtcgaggtgc agatagatgt
     2281 gatcgttctg cctgccgacg ccgcgtccct cgcggatttc catggtcatg cagcgcgaga
     2341 cgaaatcgcg cggcgccaga tccttgtagg tcggcgcata gcgctccatg aagcgctcgc
     2401 cggcggaatt ggtgagataa ccgccttcgc cgcgcgcacc ctcggtgatc agacagcccg
     2461 agccgtagat gccggtcggg tgaaactgga cgaattccat gtcctgaagt ggcagaccgg
     2521 cccgcgccac catgccgccg ccgtcgccgg tgcaggtatg ggcggaggtc gccgagaaat
     2581 aggcgcggcc atagccgccg gtcgccagca cgaccagttt ggcgcggaag cgatgcaggg
     2641 tgccgtcgtc gagcttccag gcgatgacgc cctggcactc gccatccttc atgatcaaat
     2701 cgagcgcgaa atactcgacg aagaattcgg cgttgttgcg cagcgactgg ccgtacagcg
     2761 tatgcaggat ggcgtggccg gtgcggtcgg cggcggcgca ggtgcgctgc accggcgggc
     2821 cctcgccgta ttcggtggtg tggccgccga acggacgctg gtagatcttg ccttcctcgg
     2881 tgcgcgagaa cggaacgccg taatgttcca gctcgtagac ggccgccggg gcttcccgcg
     2941 ccagatattc catcgcgtcg ttgtcgccga gccagtccga tcccttgacg gtgtcgaaca
     3001 tgtgccactg ccagcagtcc ggccccatgt tggcgagcga ggcggcaata ccgccctgcg
     3061 ccgccaccgt gtgcgagcgg gtggggaaca ccttggtgat gcaggccgtg cgcaggccct
     3121 gttcggccat gccgagcgtg gcgcgcagac cggcgccgcc agctccgaca accaccacgt
     3181 cataggcgtg atcgacaagg gggtaggcgc gtccgttgac tgccggtgac gctgacttgg
     3241 ccatgatcac aacccgaagc tgatacgaag aagcgcatag accgaggcga gcccgaccaa
     3301 agcggcaaag aaggtgttga gcatcagaca gaccagcttg acgccctcgc cgtgcacgta
     3361 atcctcgatg atcacctgca tgccgacgcg catgtggtag acgccggtca gcacgaaggc
     3421 gagcatcagg atcgccgtca gcggtccctt gagaatggca accgcgccgg catgatcggc
     3481 cccggcaagg cggatcacgg tgatgatgaa gatcagaacc agtgggatca gcgcgacgcc
     3541 ggttatccgc tgtgcccaga aatggccggt gccggtcttg gcggcgccga gacccttcac
     3601 gcgggagcgc ggcgttgcca tggaagagga ggagtttttc atcgccgacc ttccttattt
     3661 gccgaagccg aaaatcagcc acagcgccag agtcagcgcg accgaaccga ccgccgtcgc
     3721 cttgacgatg gtttcgcgtt cagggtggcc aaggccggcg ccggtatccc agatgaaatg
     3781 gcggataccg ccgagcatgt gatggatcag cgcccaagta tagccgaaca ggaccagttt
     3841 gccgaggaag gagccataga tggcgccaac catcgcatgg gcatccttgc cggcggctgt
     3901 ggccacaaga aatccaacca gcagcagggt gccgaaatac agcgcagcgc cggtgatgcg
     3961 atggacaatc gacatcatca tcgtcagcgt cggcttgtag atctgcagat ggggcgaaag
     4021 cggccgttcg gccggtgtct tggcgtctgc catcattgtg cccttgatcc gaggaaggct
     4081 gggggttctt tccactagcc aatctccgcc gcaggcgcaa ttgccgatcc cccctacatt
     4141 cggtgtaagg ccggctttgc gcccgccggg cgtccggttt agccggtggc cgcaaccaac
     4201 cgctggcgca gcgtgtccgg atcgccgtcg atgcggatcg tcttgaggcg ctgcgccggt
     4261 ccgccgatca gctggacggc cgaccgcgga acgccggccg tttcggccag cagtctttca
     4321 agcgccgcat tggcggcgcc atcctcggga atggcgcgca cccggaccgc gagcacggtc
     4381 gagccgtccg aaagcgtccg cagcccgtcg atccgggaat cgtgtgcgtt gggtgtcaga
     4441 cggatgcgaa cgatcacatg gtcagagccg gcggtgaagg gcggtgtgct cacagcgccc
     4501 gagccagcga ggaaaccatg tcgtggacga ggatctggat gaaatagagc ccgaccagca
     4561 ggatcagcgg cgagatgtcg atgccgccca gatccggaag ccgccgccgg atcggcccga
     4621 gcaccggctc ggtcacccga tagaggaact cgctcagttg catcacgaac ggctggcggg
     4681 cgttgacgat gccgaactgg atcagccaac tcatcacgat ctggccgatc accagccaga
     4741 tatagatcga gatgacagta tcgaagagcc agagaaatgc gcgcatggtt gacccgttgg
     4801 taaagcggtt tcctgtcata ctccacaaat cggcatgagc aagccaccgg agccgcaccg
     4861 aaggcagttc ccgaaggctg ggtcgcgttg cccgaatgac ctgaaggatt gacctgcgag
     4921 atcgacttgc gggattgact tggttgccga actgacgcaa gagagcgccg ccttggggct
     4981 gtagctcaga tgggagagcg ctgcaatcgc actgcagagg tcaggggttc gaatcccctc
     5041 agctccacca gggttcccgt tttatcgggt cttgagaggt cgattgccga tcctcaatca
     5101 attccgcctc acatgagctt ttccccgcaa tgggcgcaga agcggtccgt ctcgccggtt
     5161 ggcttgccgc actggtggca gaagcgtctt ggcgcctgtg ctgcttttac cggcgcaacc
     5221 gggtccggcg ccggctttcc aaatcctgac aattctccaa ccagataggc ggtcacgctg
     5281 tggcgatctg tccgggcaag ccggacaagc cctgccgccg tcaacgttac ctcaaacagc
     5341 gttccgccgg tgccggctag cagaatgcgt tcgtcaagcg ccagaacccc ctcgccggtc
     5401 agtgctgccc gctgccactg cgcgccggcg accagaccaa gggccgcagc gctcagcggc
     5461 tcgagcatcg cagccggcat caggcgctgc ccgtccccga ccaccaccag cccggcagcc
     5521 tgcagtcgct gcactgcttc tgcgacttct gccgccgcgg gcgcctcggg ccggccgccc
     5581 agcgcaacca cataagtgag aagctggtcg aagcgcgaca ggccccacca gttggtcagt
     5641 gccgaaagca gatgttcggt ggtgagtggg ctggcatcag ccagcgcaga gtcagccagg
     5701 gtggcgccat cgagggcgcg cgcgacccgg cggctgcgaa taaccgcgct cgtttcatcg
     5761 acgaggactg ccagcgttgc catcgctacg acatctagtt gcagcacgaa cggaggcgct
     5821 gccggtgccg ggggcagcag cggggcgatc aggcgcagca gatcatgcgc ctcaaccggg
     5881 ccgcagacct cacattctcc ggcatggtcg ttcagagtgt agccgccacc cgtcgccgga
     5941 tcgcccggaa acaggatcgt ggtctcggcg caggccgcct cgtcactcca caggcgcagg
     6001 gtgatgcggc tgcgcggatt gctcaagacg ccggccgcat cgcgcaggcc atcggttgcg
     6061 ccggggcggg caacatgcgc agcgcccatc agccggcgcg gcgacaacgg cccgcaggcg
     6121 ccgcaatcgt cgatcacccc ggccagatcc tcccgggtga ccctgatgca gcgcgcggat
     6181 gatgaaaacg gcatgacctt cccctaccgg tccgagccaa ggcggagggc ctgacgaagc
     6241 gccgcacgcg ccgcttcgcc tgccggcagc agcggggcag ggccgtccgg ggcggcagct
     6301 cctgaccctg tcgcgccgaa tttggcgacg aattcgggca tctgcgccag tttgtcggcg
     6361 ccggcagaca ggtccatgcc ggtcagtgcc cggaatcggg cattgccggt gcccgggggc
     6421 atgtcgttgt tggcgacggc ccgcatgata tcgagcgggg tctcgccggt aacggcatcg
     6481 cgcactgcaa agaccggcgg caggcgagac ccggatttgg cctccatcag cggccgggtg
     6541 atccggtcga aatccttggc ggcgatacgg cacttttccg agacggcgcc agctcccgta
     6601 tgcaggacgt ctgcctccgc aagcttggca ccggtgactt ccgccagcga gcgcccttcg
     6661 gcgcccgtca cattgccgga ggccacgcgg gggatgaatt ttgccggatc ctcgcccggc
     6721 cgcagcgtgt agcgctccag gctttcgccg tgcatcagat tgatttcctg gccggatttg
     6781 ctgattccaa gcctggtgca ggctctgcca acggctgcgt caaggtccgc agcacgcgct
     6841 gggcggccgg tcgtgcggtc gatgatcgtc gcattcgcgg tcatgtcgag gtccatgccc
     6901 ggcgccttgc cggcggtgcc gctggtgctg cgaaaatcgg ttggcaacac cttgcgttca
     6961 agaaatccgc cggggttggc cggatcgggc tcgcgcacta cgaagcgctg ggctccgtcg
     7021 gttcccttct gctggttcag atgatcggcc gtggcagcga acaccggttt cgtacggtgc
     7081 tgttcggtga ggtcggcgaa ggccgtcttg gtcgctgcat ccacctcggc gcctttcatc
     7141 agcagcttgg catcgcggtt ttccaggacg cgtttcagtg cttccttctg ctcgtcgggc
     7201 gttccgctgg cgatggcacg tttgagatca tcgaccaccc cctgcgcctg ggtcttgcgg
     7261 acggcggcat cggcgtcgag ctgaatgtct gctgcggtca gtttcgggtc ggtttgcacc
     7321 gggcggaagg tttcgctccc tggcaggggc agcggttcgg cccgggcgac gccagccttc
     7381 gccgcatgac caccgacgga tgatccgaca ccgctcgcca agccgtcaag gaacccttcg
     7441 gtgacattgc catccgtcgt cgcgcgcccg gcggcaccca tgactccgcc aagcgccact
     7501 tgtgctcctt cgcgcgcgac cgtgctgccg agcctgccca aaccctgccc gg
//
LOCUS       DAOMXG010000119         7504 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_5126_length_7504_cov_10.416834, whole genome shotgun sequence.
ACCESSION   DAOMXG010000119 DAOMXG010000000
VERSION     DAOMXG010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 7504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7504
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5126_length_7504_cov_10.416834"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="PLQ11_08815"
     CDS             complement(<1..853)
                     /locus_tag="PLQ11_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25038.1"
                     /translation="MTLALALLDPDAPVSALGRPGLHEIYAGTLLDTVSALGLALGLV
                     RGRGAGKPWLWVWHEALDHAFGRPFPPGLAEWGLVPSSLVLVRVQSVREALQAGLDAA
                     RCAGLGAVVISLSGEARSYDLVATRRLAMAARARRNCVFIVRAGAAPVPSAAETRWRA
                     GASPSSVLAAQAPGMPALRLELLRHRGGCAPGFIVMEWNRDAGCFRGRARAGCTAGTP
                     SGAPLSGALVPMVLGRTDHPQAPERGGGRIGGCPAAGAGEGPVWRVAAGGARSGGLSA
                     WPAHRHES"
     gene            1201..2616
                     /locus_tag="PLQ11_08820"
     CDS             1201..2616
                     /locus_tag="PLQ11_08820"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
                     /protein_id="HRK25039.1"
                     /translation="MGGASNFRNFLLGACAIGSLALATVSAEAGGFANRQQSATGQGF
                     SFAGAGTSAFGLGSMFWNPANITNFEGRRSEWNVSMIAPDSRVDTTSAGFGPAALQPL
                     NAFVTGRTGSSGNINQTAVAPASYNSYQYNDWLWLGLVTGTPFGNTTKPSNGFAGSVY
                     GTSTKIKTLAATPTIGIKINDWISVGFGFTVQHARVDLKSGDMRYWPAIALAPALFQP
                     NLYRASAPTRLEGDAWGFGVTAGVTLKPWAGGEVSLGYRSMIHHALKGSISAYGPTLT
                     TTGQLIKANLNLPEMITLGLKQDINAQWTVTATAQWTNWSRLQAPPIINRTTGALVSN
                     LGLRYRDEWFLAAGAQYNWSPNLALRAGVAYEISPITDETRGVRVLDNNRLWLSAGLG
                     YKVSNRLELDLGYSFLLLESGKVNIVAAGNPLNPAGNPSYTAFNYTGNSKGHGHVLAA
                     SLKYRWDEPANAPIKPVVAKN"
     gene            complement(2746..3474)
                     /locus_tag="PLQ11_08825"
     CDS             complement(2746..3474)
                     /locus_tag="PLQ11_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015930271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRK25040.1"
                     /translation="MRAEDEKGVGFRPLYRQVKDTFVKRMADGQWPPGHPLASESQLA
                     SELGVSQGTVRKALDELESENLVVRQQGRGTFVAQHDEKRILFQFFKLIKNDGIPRFP
                     ESQVLSAGVFKATAEEREGLDLGSSAMVARVRRVRFIADEPVIVETLSLPDAMFPGIT
                     EVPVPNSLYAYYAERYGITIASAREKITAIALQPRDATILDRDAGHPALMVQRLALAL
                     DGRAVEWRVSLCLTDTMHYASSLR"
     gene            3606..3893
                     /locus_tag="PLQ11_08830"
     CDS             3606..3893
                     /locus_tag="PLQ11_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HRK25041.1"
                     /translation="MSVPHLLVHEHKDTVGVVVVENLKAGTDMLAVVTHDNSAFNIAA
                     KMDIPIGHKVALKDIRKGDTIWKYGQDIGKAVADIGKGEHVHVHNVKTKRW"
     gene            3967..5226
                     /locus_tag="PLQ11_08835"
     CDS             3967..5226
                     /locus_tag="PLQ11_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013421107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HRK25042.1"
                     /translation="MIKTRLKAVKGRRIEAPDFKAPAIAPRRGAAATDYFWGWRRENG
                     RVGVRNHVVLLPLDDLSNAACEAVANNIKGTLALPHAYGRLQFGADLDLHFRTLIGTG
                     ANPNVAAVIVIGIEDSWTRTVVDGIAATGKPVVGFGIEGHGDIAVIAKASYVAKEFMQ
                     WASELQREKCPVSELWVSTKCGESDTTTGLSSCPTVGNMYDKLIPKGIYGVFGETSEI
                     TGAEHLCKERAANKEVGERWFRMWKAYQDDVIEAHKTSDLSDSQPTKGNIAGGLTTIE
                     EKALGNLEKIGRECRYIDILEPAEAPRKGPGLYFMDTSSAAAECVTLMAAGGYVVHTF
                     PTGQGNVIGNPIVPVIKITGNPKTMRTMPEHIDVDVSGVLKRTLTIPEAGDALIANII
                     RTANGRLTAAEALGHREFVMTKLYRSA"
     gene            5228..6274
                     /locus_tag="PLQ11_08840"
     CDS             5228..6274
                     /locus_tag="PLQ11_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HRK25043.1"
                     /translation="MASAGANTESGPAALLPGLLLSAGVAIAAVAAEPALKFMTGGRL
                     ALPAMVLALVIGIALNRFAVKAAFQPGLTYAVKILLRYTIALLGLRIAFADFVALGWQ
                     TVLVVIVAMAATVAAGLITARLLGQQNGLGALCGAACAVCGASATLATATVVPDYRTR
                     SADIAFTVVMANAISTVVMLAYPWLCSALGLGAQQTGLMLGLTIHDMAQVAGAGYAIS
                     DPVGNTAVIVKLLRVLLLLPVVLAIGHWLARQGNATGPAQVPVPGFAVVFILLAIVNS
                     ILPGTALASAYAPLKAALATLSNWGLLIAISALGLGSSMAAIMSIGWRHLVVFLVPTG
                     VILAIGLAALPILR"
     gene            6278..7210
                     /locus_tag="PLQ11_08845"
     CDS             6278..7210
                     /locus_tag="PLQ11_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008597145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HRK25044.1"
                     /translation="MPDIIIPEFMDEAAVASLSARHDTLYDPGLVDQPERLIGLIGGA
                     RALIVRNRTQVRGALLDAGEKLEVVGRLGVGLDNIDVAACAARRLPVIPATGANDLAV
                     AEWVIASTLMLLRGAYHATDAVIAGQWPRNRLMGREASGRIMGLVGFGAIAREVAIRA
                     RALGMRVIAHDPLLPEGHAAWALAERRDLEPLLAAADIVSLHVPLTEATRAMIDERRI
                     GLMRPDAVLLNAARGGVVDEAALAKALRAGRLGGAALDVFADEPLSASGAAMFIGCPN
                     LILTPHIAGVTQESNGRVSSLIAARILDVLGGRA"
     gene            7207..>7504
                     /locus_tag="PLQ11_08850"
     CDS             7207..>7504
                     /locus_tag="PLQ11_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019962879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HRK25045.1"
                     /translation="MSEERLSIAAAEALVSQALQRCRTASDTAETVARALVAAEADGL
                     KGHGLSRVPSYAAQARIGKIDGFARASVETVTPALLRIDAGLGFAYPAIDLAITG"
BASE COUNT         1351 a         2484 c         2343 g         1326 t
ORIGIN      
        1 cagactcatg ccggtgcgca ggccaagctg ataagccgcc cgatcgagcg ccgccagccg
       61 caacccgcca gactgggcct tcaccagcac cagcggcggg gcatccgcca atccggcctc
      121 cccctctctc cggcgcttgc gggtgatccg ttcggccgag aaccatgggc accagagcgc
      181 cagataacgg cgcccccgag ggtgtgccgg cggtgcagcc agcacgggcg cggcctctga
      241 aacatcctgc atcgcggttc cactccatga cgatgaagcc gggcgcgcag ccgccccggt
      301 ggcgcaacaa ttcaagcctg agagccggca ttcccggcgc ctgcgcagcc aagacggaag
      361 aaggagaggc cccggcccgc cagcgcgtct cggcagcact tggaaccggt gcggctcccg
      421 ccctgacgat gaacacgcag ttccgccgag cccgtgctgc catcgccagc cgccgggtgg
      481 cgacaagatc atagctgcgc gcctcccccg acagactgat caccactgcg ccgagcccgg
      541 cacagcgtgc cgcatcaagc ccggcctgca gcgcttccct gacgctttgc acccgcacca
      601 gcaccagact ggacggaacc agcccccatt cggccagacc gggcggaaag ggccgcccga
      661 acgcgtggtc caaagcctcg tgccacaccc aaagccatgg cttgccggct ccccgcccac
      721 gcaccagccc cagcgccagc cccagcgcac tcaccgtatc cagcagcgtc ccggcataga
      781 tctcgtgcag gccgggccgt cccagagctg agacaggcgc atccggatcg aggagggcga
      841 gcgcaagcgt cacgattttt atccctattt gttcttttat tgttcttttt tagttcttgt
      901 ttgtcaatat cgaagccaga tcgtgctcgg catgatgcgg cgccggcgct tcggccacaa
      961 agctcgcagc cgacagaccc ggcccatcca gcagcctgag gagcagcgcg aaacatgttg
     1021 caagaaagcc actttggccc acaaaggcag ggaaatgccc ttaaagacag gcaaattgcc
     1081 tcctcaacga ttgcgtggcc gggaaagtca ggcaattaag ttgttaacta tcttgcgggc
     1141 gactcgtccg cgtcgggtta tccaaagatc aacagcaaac gcagcatcgg ggaacaccaa
     1201 atgggcggcg cttctaactt caggaacttc cttctcggcg cttgcgcgat tggctcgctc
     1261 gcactggcca cggtctctgc agaggccggc ggctttgcca accggcagca gagcgcaacc
     1321 ggacagggct tttcgttcgc cggcgccggc accagcgcct tcggcctcgg ctcgatgttc
     1381 tggaaccccg ccaacatcac caatttcgaa ggccgccgca gcgagtggaa cgtctcgatg
     1441 atcgcgccgg attcgcgcgt cgacaccacc agcgccggct tcgggccggc ggcgctgcag
     1501 ccgctcaatg ctttcgtgac cggtcgtacc ggctcgtcgg gcaatatcaa ccagaccgcg
     1561 gtcgctccgg catcctacaa ttcctatcag tataacgact ggctctggct tggtctcgtg
     1621 accggcacgc cgttcggcaa cacgacgaag ccgagcaatg gctttgccgg ctcggtctac
     1681 ggcaccagca ccaagatcaa gacgctggcc gcaaccccga ccatcggcat caagatcaac
     1741 gactggattt ccgtcggctt tggcttcacg gtccagcacg ccagggtcga tctcaagagc
     1801 ggcgacatgc gttactggcc ggccattgcg ctggcgccgg cactcttcca gccgaatctc
     1861 taccgcgctt ccgcgccaac ccggctggag ggcgatgcct ggggcttcgg cgtgaccgcc
     1921 ggtgtgaccc tgaagccctg ggccggcggc gaagtcagcc tcggctaccg gtcgatgatc
     1981 caccatgcac tcaagggctc gatctctgct tacgggccga ctctcaccac caccgggcag
     2041 ctgatcaagg ccaacctgaa cctgccggag atgatcacgc ttggcctcaa gcaggacatc
     2101 aatgcccagt ggactgtgac cgccaccgcg cagtggacaa actggagccg cctgcaggct
     2161 ccgccgatca tcaaccgcac cactggcgcg ctggtcagca atcttggtct gcgctaccgc
     2221 gacgagtggt tcctggcggc gggtgcccag tataactgga gcccgaacct ggcgctgcgt
     2281 gccggcgttg cctacgagat ctcgccgatc accgacgaga cccgcggcgt gcgcgtgctc
     2341 gacaacaacc gtctgtggct gtcggcgggt cttggctaca aggtttccaa ccgtcttgaa
     2401 ctcgacctcg gctacagctt cctgctgctg gaaagcggca aggtcaacat tgtcgcggct
     2461 ggcaacccgc tcaacccggc agggaacccg tcctatacgg ccttcaacta taccggcaac
     2521 tcgaagggcc atggccatgt gctggcggcc tcgctcaagt atcgctggga cgagccggcc
     2581 aacgctccga tcaagccggt cgtcgccaag aactgatcca agaactgatc acggcgcccg
     2641 aaagggcgac ccgtcaggac cccgcctgtc acaaggcggg gtttttcttt ggcggaacat
     2701 cgtcgggaaa cggccggttg tggaggttgc agccggagtc aggacctatc gcaatgagga
     2761 ggcgtagtgc atggtgtctg tcaggcacag gctgacgcgc cattccacgg cgcggccatc
     2821 aagcgccagc gccaaccgct gcaccatcag cgcagggtgg ccggcgtcgc ggtccagaat
     2881 agtcgcatcc ctgggttgca gcgcgatggc ggtgatcttc tcccgggcag aggcaatggt
     2941 gatgccatag cgctcggcgt aataggcgta gagtgaattg ggcaccggca cttcggtaat
     3001 gcccggaaac atcgcatcag gcagcgacag ggtttcgaca atcaccggct catcggcgat
     3061 aaaacgcacg cgccggacgc gcgccaccat ggccgaactg ccgagatcaa gcccctcgcg
     3121 ttcctcggct gtcgccttga acacgcccgc cgaaagcacc tggctttccg gaaagcgcgg
     3181 aatgccgtca ttcttgatca gcttgaagaa ctgaaacaga atccgttttt cgtcatgctg
     3241 ggcgacaaag gtgccgcgcc cctgctgacg caccaccaga ttttctgact ccagctcgtc
     3301 cagcgccttg cgcaccgttc cctggctgac gcccagctca ctggccagct ggctttcgct
     3361 cgccagcgga tgccctggcg gccattgtcc atcggccatg cgcttgacga aggtatcctt
     3421 cacctgtcgg tagagcggcc ggaacccgac gcctttttcg tcctctgcac gcatgcccct
     3481 ctgctccatc ttctgacttg ccagaagtct tttgtcttat ataagatata gcatgttttg
     3541 ccgctcatcg ccagtccggc cgccccatcg ggacggcggg accgaccgaa agggagaagg
     3601 attgtatgtc agtaccgcat ctgctcgttc atgaacacaa ggacactgtc ggggttgtgg
     3661 ttgtcgagaa cctcaaggcc ggcaccgata tgctggcggt cgtcacccac gacaattccg
     3721 ccttcaacat cgcggccaag atggacatcc cgatcggcca caaggtcgcg ctcaaggata
     3781 tccgcaaggg cgacacgatc tggaaatacg gccaggacat cggcaaggcc gtcgccgaca
     3841 tcggcaaggg cgagcatgtc cacgtgcaca acgtgaagac caagcgctgg taagcgccgc
     3901 tcaccattcc agcccaatca caaggaacgc accatggcca gcgcaccgat cgtcgccaat
     3961 ttttccatga tcaagacccg cctgaaggca gtgaagggtc gcaggatcga agcccccgat
     4021 ttcaaggccc ccgccatcgc gccgcgccgc ggggcggctg cgactgacta tttctggggc
     4081 tggcggcgcg agaacggccg cgtcggtgtg cgcaatcatg tcgtgctgct gccgctggac
     4141 gatctgtcca acgcggcctg cgaggcggtc gccaacaaca tcaagggcac gctggcgctg
     4201 ccgcatgcct atggccgcct gcaattcggc gctgatcttg acctgcattt ccgcacgctg
     4261 atcggcaccg gcgccaatcc gaacgtcgcc gccgtcatcg tcatcggcat tgaagattcc
     4321 tggacgcgca cggtggtcga cggcatcgcc gcgaccggaa agccggtggt cggcttcggc
     4381 atcgagggac atggcgacat tgcggtgatc gccaaggcct cctacgtcgc caaggagttc
     4441 atgcaatggg cctcggagct gcagcgcgag aagtgcccgg tatcggaact ctgggtttcg
     4501 accaaatgcg gcgagagcga cacgacgacg ggcctttctt cctgccccac tgtcggcaat
     4561 atgtatgaca agctgatccc gaagggcatt tacggcgtgt tcggcgagac gtccgagatc
     4621 accggcgccg agcatctgtg caaggagcgc gcggcgaaca aggaagtcgg cgagcgctgg
     4681 ttcaggatgt ggaaggccta tcaggatgat gtgatcgagg cgcacaagac ctcagacctg
     4741 tccgacagcc agccgaccaa gggcaacatt gccggcggcc tcaccaccat cgaggaaaag
     4801 gccctcggca acctcgaaaa gatcggccgc gaatgccgtt acatcgacat tcttgagccg
     4861 gccgaggccc cccgcaaggg gccgggcctc tacttcatgg atacatcctc ggcggcagcc
     4921 gaatgcgtga cactgatggc ggcgggcggc tatgtggttc acaccttccc caccggccag
     4981 ggcaatgtca tcggcaaccc gatcgttccg gtcatcaaga tcaccggcaa tcccaagacc
     5041 atgcgcacca tgcccgagca catcgacgtg gacgtctccg gcgtgctcaa gcgaaccctg
     5101 acgatcccgg aagcgggcga tgcgctgatc gccaacatca tccgcaccgc caatggccgg
     5161 ctgaccgccg ccgaggcgct gggccatcgc gaatttgtca tgaccaagct ctaccgctcc
     5221 gcctgacatg gcatcggcgg gggcaaacac cgaaagcggc ccggcagcac tcctgccggg
     5281 cctcttgctg tcggcggggg tagcgatcgc ggcggttgcc gccgaaccgg cgctgaagtt
     5341 catgacggga gggcgcctcg cattgccggc catggtgctg gcgctggtca ttggaattgc
     5401 tctcaaccgg ttcgcggtca aagccgcatt ccagccggga ctgacctatg cagtcaaaat
     5461 cctgctgcgc tatacgatcg cgctgctggg cctgcgcatc gcctttgcgg attttgtcgc
     5521 acttgggtgg cagaccgtgc tggttgtcat cgtcgcgatg gcagccaccg tcgccgccgg
     5581 gttgatcacc gcccggttgc tgggccagca gaatggcctg ggggccctgt gcggcgcggc
     5641 ctgtgccgtc tgcggcgcct cggccacgtt ggcgaccgcc acggtcgtgc cggattatcg
     5701 caccagaagt gccgatattg cctttaccgt cgtgatggcc aatgccatct caaccgtggt
     5761 gatgctggcc tatccctggc tgtgttcggc gcttgggctc ggcgcacagc aaaccggcct
     5821 gatgctggga ctgacaatcc atgacatggc gcaggtggcc ggcgccggat atgccatctc
     5881 cgatccggtg ggcaacacgg cggtgatcgt caagctgttg cgggtgcttc tgctgttgcc
     5941 tgtggttctc gcgatcggtc actggctggc acggcagggc aatgctacgg ggccggcgca
     6001 ggtgcccgtt cccggctttg ccgtcgtgtt catcctgctg gccatcgtca attcgatcct
     6061 gcccggaacc gcgctggcct cagcctatgc gccgttaaag gccgctctcg ccaccctgtc
     6121 gaactggggg ctgctcatcg ccatttccgc actcgggctc ggctcctcga tggccgccat
     6181 catgagtatc ggatggcgcc atcttgtcgt attcctggtg ccaaccgggg tcattctcgc
     6241 gatcggcctc gccgcactgc cgatcttgag ataaccgatg ccggacatca tcatccccga
     6301 gttcatggat gaggcagccg tggccagcct cagcgcccgg catgacacgc tgtatgaccc
     6361 cggactggtt gaccagccgg agcggctgat cggattgatt ggcggggcgc gggctttgat
     6421 cgtgcgcaac cgcacccagg tgcgcggcgc cctgcttgat gccggagaga agcttgaagt
     6481 cgtcggccgg ctcggcgtcg gcctcgacaa catcgacgtc gccgcctgcg ctgcacgccg
     6541 cttgccggtg atcccggcca ccggcgccaa tgacctggct gtagccgaat gggtgattgc
     6601 ctcgacgctg atgctgctgc gcggcgccta tcatgcgacc gatgcagtga tcgccgggca
     6661 atggccgcgc aaccggctga tgggtcgcga ggcatcgggc cgcatcatgg ggttggtcgg
     6721 tttcggcgcg attgcgcggg aggttgcaat ccgcgcccgc gcgctgggca tgcgggtgat
     6781 cgcccatgat ccgctgctgc cggaggggca tgcggcctgg gcgctggcgg agcgccgcga
     6841 tctcgaacca ttgctggcgg ctgccgatat cgtgtcgctg cacgtgccgc tgaccgaggc
     6901 gacgcgggcg atgatcgacg agcggcgcat cggcctgatg cgaccggatg cggtgctgct
     6961 caatgcggcg cgcggcggcg tggtggatga ggcagccttg gccaaggcgt tgcgcgcggg
     7021 acgccttggc ggggccgcgc tcgacgtgtt cgccgacgag ccgctgagtg cgagcggtgc
     7081 agcgatgttc atcggctgcc ccaaccttat tctgaccccg catatcgccg gggtgactca
     7141 ggagtcgaat ggtcgggttt cgagcctgat cgcagccagg attcttgacg tgctgggagg
     7201 cagggcatga gtgaggaacg gctgtccatc gccgccgccg aggcgctggt atcgcaggcg
     7261 ctgcagcgct gtcggaccgc gtccgatacc gccgaaacgg tcgcccgcgc gctggtcgcg
     7321 gcggaagcgg acgggctgaa aggtcatggg ctgtcgcggg tgccgagtta tgccgcgcaa
     7381 gcgcgcatcg gcaagatcga cgggtttgcg cgcgcctcag tggagacggt cacccctgcc
     7441 ctgctgcgca ttgatgccgg cctcggcttt gcctatccgg ccatcgacct tgccatcact
     7501 ggtt
//
LOCUS       DAOMXG010000120         7485 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_5141_length_7485_cov_9.511036, whole genome shotgun sequence.
ACCESSION   DAOMXG010000120 DAOMXG010000000
VERSION     DAOMXG010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 7485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7485
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5141_length_7485_cov_9.511036"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="PLQ11_08855"
     CDS             <1..658
                     /locus_tag="PLQ11_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25046.1"
                     /translation="PAASPALEVNNPMLDALVYGDNDLVGLVAYAMHEVHRRDWCRAY
                     EKSNARLPAEADLNAYLVGEQLDRRIEAYRRLAEDALAKVASGNTDMTMRRLAEGVPE
                     VTVGHIASVPPLAAPAEAAAAAPVADAAPAQKAEAHAPQPTLASTPATPPAATIPTPA
                     TALIRQPMLGATPADKAIVPIASGKTTKLIAYLGFLLVAVIMLALLMKWGMDITQPTR
                     "
     gene            complement(665..1762)
                     /locus_tag="PLQ11_08860"
     CDS             complement(665..1762)
                     /locus_tag="PLQ11_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HRK25047.1"
                     /translation="MIGSHAPCSAPIAHALRRGRGAVSNVSGRFERCSRSPVFDEATE
                     FEDLAPFRTDVAVERARTIITRNDSPDLGFDRSINPYRGCEHGCSYCYARPSHAYLGL
                     SAGLDFETRLFAKPNAAELLERELSEPGYQPKLIALGANTDPYQPIERDWKITRAILQ
                     VLAEARHPVGIVTKSALVMRDIDILAPMAAQGLVKVALSITTLDRSLARAMEPRAATP
                     SRRLEAIRQLSAAGIPVSVLMAPVIPALTDHEIEQVLTAARAAGAVEAGYILLRLPLE
                     VREVFSQWLVSHAPDKARHVLSLIRSTRGGKDYDSRFFARQTGEGPFAWLIGRRFELA
                     AERLGFARKRMRLRTDLFRPPRGGTGQLSLF"
     gene            2122..2571
                     /locus_tag="PLQ11_08865"
     CDS             2122..2571
                     /locus_tag="PLQ11_08865"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRK25048.1"
                     /translation="MYKKILVAVDLSELDIAQPALAAAIELATLTSGELRLVHVRHRL
                     PSAPISVMAADLLSDQEQYFKGEMDRLMAKVPYPKERLSSANLIGTVYAEILQEAKSW
                     GADLIVLSAHRPSMATYLLGSNAAKVVRHAECTTLVVRSHKPLSLMA"
     gene            2658..4307
                     /locus_tag="PLQ11_08870"
     CDS             2658..4307
                     /locus_tag="PLQ11_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HRK25049.1"
                     /translation="METGHSKFDRGLDRNAANHVPLSPISLIARTAAVHPGLPSVVHE
                     GRRFTWAQTYERCRRLASALARLGIGKNDTVAAMLPNIPALYEAHFGVPMCGAVLNTL
                     NTRLDAEAIAFMLDHGEAKALLVDPEMAGVVEAALGLMQGPRPTVIDVADAAFPGAKP
                     IGAIEYEAFLATGDPDFRWQHPADEWDAIALNYTSGTTGNPKGVVYHHRGAYLNAVSN
                     VLAASLPAHPVYLWTLPMFHCNGWCFPWTIAALAGVNVCLRKVEATKVFALIREEGVS
                     HMCGAPIVYNMLINAPDAPRGTEARKVVGLIAGAAPPMAVIAGSESIGIAITHVYGLT
                     EVYGPAAVCSSQPGWSDLPLEERARLNRRQGVPYHLQESVTVMNPETMAEVPADGETM
                     GEIMFRGNIVMKGYLKNPKATEEAFAGGWYHTGDLAVRDPDGYVMIKDRSKDIIISGG
                     ENISSVEVEDVLFRHPAVLLAAVVAKPDPKWGEVPCAFVELKDGASATEAEIIAWCKQ
                     HLAGFKTPKQVVFGVIPKTSTGKLQKYVLRQRVGSAAAITA"
     gene            complement(4375..5478)
                     /locus_tag="PLQ11_08875"
     CDS             complement(4375..5478)
                     /locus_tag="PLQ11_08875"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HRK25050.1"
                     /translation="MLEAIIERWGEPLIGLASGALIGLIFGILAQRSRFCMRSAVIEV
                     GRGHAGPSLALWLLAVGTAIAGVQGLMLAGVISADPIRMLNGGASLSGAAVGGLLFGI
                     GMVLSRGCVSRLLVLSASGNFRSIVTATVFVATAYATMKGPLGDARAAIAGLWQVEGR
                     GALDLTAATGLGARGALLSGLGLIVAALVLGARRRLSLERMIMGIAIGAIVAGTYALT
                     GLMNAASFDPQPVRGLSFIAPAVNATTSALSLPGGRVDFDFGLIPGVIVGAFLAALLS
                     GSLRREWFHRPIDGLRYLSGAVLMGFGGVLAGGCSMGNGVSGWAIYATGALVALAAMW
                     IGALLADALIDRPATARTSAAAPLETASAAFRI"
     gene            complement(5838..6746)
                     /locus_tag="PLQ11_08880"
     CDS             complement(5838..6746)
                     /locus_tag="PLQ11_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HRK25051.1"
                     /translation="MRLDDVGESTNVEDRRGDGGGGFGGGFGFPMRSGGLGFGTMIVL
                     SLVAWAFGISPSILIGGAEMLSNVTGGSRTEQVSAPRPAGAKPDADQQFVARVLKSTE
                     DVFTEQLAKQLNIKYQKPNLVMFSNFTRSGCGGAQSAMGPFYCPLDSKVYLDTAFFGE
                     MKTRLRAGGDFAYAYVIAHEVGHHVENQLGILPKVQEMQQRVGRKESNNLSVRVELMA
                     DCLAGVWAHHAEQKFRILEQGDIEEALNAASQIGDDKLQKASQGYAVPDSFTHGSSQQ
                     RMEWFTRGLKTGQIRACDTFNARSGV"
     gene            complement(6876..>7485)
                     /locus_tag="PLQ11_08885"
     CDS             complement(6876..>7485)
                     /locus_tag="PLQ11_08885"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRK25052.1"
                     /translation="EAERGARDRAYSAASLADLRALMEAFDGCALKHSARQLVFADGN
                     PQARIMLVGEAPGRDEDEQGLPFVGRSGRLLDRMLAAIGLDRTSVYITNIIPWRPPGN
                     RTPTPQEIAICLPFIARHIALVDPVVVVCLGALSAQTLLGQRDGILRLRGRWFDYDTG
                     HRTIPALATLHPAYLLRQPAQKRFAWRDLRALAARFAEIEQK"
BASE COUNT         1437 a         2425 c         2378 g         1245 t
ORIGIN      
        1 gccggcggca tcgccggcgc tcgaggtcaa caacccgatg ctcgatgcgc ttgtctatgg
       61 tgacaacgat cttgttggcc tggtcgccta tgccatgcac gaggtgcatc ggcgcgactg
      121 gtgcagggct tacgagaagt ccaacgcccg ccttccggcc gaagccgacc tcaacgccta
      181 tctggtcggc gaacagctcg accgccgcat cgaggcctat cgtcggctcg ctgaggacgc
      241 gctagccaag gttgcctccg gcaatacgga tatgaccatg cgccgcctgg ccgaaggcgt
      301 gcccgaggtc acggttggcc atattgcctc ggttccgccc cttgccgcgc cggccgaggc
      361 tgccgcagcc gctccggtag ccgatgcagc accggcgcag aaggcggagg cgcacgcgcc
      421 ccaacccacc ttggccagca ccccggcaac cccgccggca gcgacgattc caacccccgc
      481 aaccgccctg attcgccagc cgatgctggg tgcaacgccg gcagacaagg ccatcgtccc
      541 gatcgcttcc ggcaagacca ccaagctgat cgcctatctc ggcttccttc tcgtcgcggt
      601 catcatgctg gcgctgctga tgaaatgggg catggacatc acccagccaa cccgctgacg
      661 aggttcagaa caggctcaac tggccggtcc cgccccgtgg cggccggaac aaatccgtgc
      721 gcaaccgcat ccgcttgcgc gcaaagccca gccgctccgc tgccagttcg aaccgtcgcc
      781 cgatcagcca ggcaaagggc ccttcgccgg tctggcgggc gaagaacctg gaatcatagt
      841 ccttgccgcc gcgtgtcgag cggatcagcg acagaacatg ccgggccttg tccggtgcat
      901 ggctgaccag ccactgggaa aacacctcgc gcacttcgag cggcagtcgc agcaggatat
      961 agcctgcttc caccgccccg gccgctcgcg ccgccgtcag cacctgctcg atctcgtggt
     1021 cggtcagtgc cgggatgacc ggcgccatca gcacggaaac cggaattccg gcagcggaca
     1081 gctggcggat cgcctccagc ctccggcttg gcgtggcggc gcgcggctcc atcgcccgcg
     1141 ccagcgaccg atcgagcgtg gtgatcgaca gcgcaacctt gaccaggccc tgggccgcca
     1201 tcggcgccag aatatcgata tcgcgcatca ccagcgccga tttggtgacg atccccaccg
     1261 ggtgccgcgc ctctgccaga acctgcagga ttgcgcgtgt gatcttccag tcgcgttcga
     1321 tcggctggta gggatcggtg ttggccccca atgcgatcag ctttggctga tagccgggtt
     1381 ccgacagttc gcgttcaagc aattcggcgg cattgggctt ggcaaacagc cgcgtctcga
     1441 agtcgagccc ggccgacagg ccgagatagg catggctggg ccgtgcataa caatagctgc
     1501 atccatgttc gcagccgcga tacggattga tcgaccggtc aaaaccgagg tcgggcgaat
     1561 cgttgcgggt gatgatggtc ctggcccgtt cgacggcaac atcggtgcgg aacggcgcta
     1621 ggtcttcgaa ttccgtcgcc tcgtcgaaca ccggcgaccg gctgcagcgt tcaaaacggc
     1681 cggagacatt gctgaccgca ccgcgcccgc gacgcagcgc gtgggcaatg ggtgcagagc
     1741 agggggcatg ggatccgatc atgtgagaca attagcagcc acaaacatac aaaacaagaa
     1801 caaaaacgaa caaaaaagga ataaacttcc ctctctcttg ttgcgctata tcaaggccgg
     1861 gtggcagaga cgcgataggc tgcagactgg cgagggaggc tgtggcctgc agtctgatga
     1921 tcgccaacgg aaatgcgcgc ggtctgtgac cgatccccct ctcaggctgc ggaccgcgcg
     1981 catgaccccg ctctctccgt cgtgcgccgg ttttttcgct ttcatgggca aggcgctggc
     2041 ctgtggccgc agatgcggtg ccggtggcct cttgctgctg tcttattgta ggatgtgctg
     2101 accgtatcag gagggcgagc catgtataag aagatactcg ttgccgtgga tctgtccgag
     2161 ctggacattg cccagcccgc acttgcggca gcgattgagc tggcaacgct gaccagtggc
     2221 gaactcaggc tggtgcatgt gcgtcatcgc ctgccctcgg ctccgatcag cgtcatggcg
     2281 gcggatctgc tgtccgatca ggagcagtat ttcaagggtg agatggacag gctgatggcc
     2341 aaggtgccct atccgaagga gcgcctgtcg tcagccaacc tcatcggcac ggtctatgcg
     2401 gaaatcctgc aggaggcgaa gagttggggg gcggatctga tcgttctgag cgcccatcgg
     2461 ccgtcgatgg cgacctacct tctcggctcg aatgccgcca aggtcgtgcg tcatgccgaa
     2521 tgcaccacgc tggtggtgcg ctcgcacaag ccgttgtcgc tcatggcctg atggagaaac
     2581 tttcctcatc ctcttgagcc gacaggacag cgcggtatcc ttccggcagg taagcaccgg
     2641 tcgtcgggag ggcgcagatg gaaacggggc acagcaagtt cgacaggggg ctggaccgca
     2701 acgcggccaa tcacgttccc ctgtcgccga tcagcctgat cgcacgcacg gcggccgtgc
     2761 atcccggact gcccagcgtg gtccatgagg ggcgccgttt cacctgggct caaacctatg
     2821 aacggtgccg gcggctggcc tcggcactgg cgcggctcgg catcggcaag aacgataccg
     2881 tggcggccat gctgcccaac attccggcgc tgtatgaggc ccatttcggc gtgccgatgt
     2941 gcggggcggt gctcaatacc ctgaacaccc ggctcgatgc cgaggcgatt gccttcatgc
     3001 tcgaccatgg cgaggccaag gcattgctgg tcgatccgga aatggcaggc gttgtcgaag
     3061 cggcgcttgg cctgatgcag gggccgcgcc cgacggtaat cgatgtggcc gatgcggcct
     3121 ttccaggtgc caagccgatt ggcgcgatcg aatacgaggc atttcttgcc accggcgatc
     3181 cggacttccg ctggcagcat ccggcggacg agtgggatgc gatcgccctc aactatacct
     3241 cgggcaccac cggcaatccg aagggcgttg tctatcacca tcgcggggcc tatctgaacg
     3301 cggtcagcaa tgtgctggcc gccagcctcc ccgcgcaccc ggtctatctg tggacgctgc
     3361 cgatgtttca ctgcaacggc tggtgttttc cctggaccat tgccgcgctt gccggcgtca
     3421 atgtctgcct gcgcaaggtg gaggccacca aggttttcgc cctgatccgc gaggaaggcg
     3481 tcagccacat gtgcggcgcc ccgattgtct acaacatgct gatcaatgcg ccggatgctc
     3541 cgaggggcac agaggcgcgc aaggtcgtcg ggctgatcgc cggcgccgcg ccgccgatgg
     3601 cggtgatcgc cggatccgaa tcgatcggca tcgccatcac ccatgtctat gggctgaccg
     3661 aagtctacgg accggcggcc gtctgctcgt cccagccggg ctggtcagac ctgccgctgg
     3721 aggaacgcgc ccgccttaac cggcggcagg gcgttcccta tcacctgcag gaatcggtca
     3781 cggtcatgaa ccccgaaacc atggcggagg tgcctgcgga cggcgagacg atgggcgaga
     3841 tcatgttccg cggcaacatc gtcatgaagg gctatctcaa gaaccccaaa gccaccgagg
     3901 aagcctttgc cggtggctgg tatcacaccg gcgatctggc agtgcgcgat ccggatggat
     3961 acgtcatgat caaggatcgc tcgaaggaca tcatcatttc cggcggcgag aacatatcct
     4021 cggtcgaggt cgaggatgtg ctgttcagac atccggcagt gctgctggcg gcggtggtgg
     4081 ccaagcccga tccgaaatgg ggcgaggtgc cctgcgcctt cgtcgaactc aaggacggtg
     4141 cgagcgccac cgaggcggag atcatcgcct ggtgcaagca gcatctggcc gggttcaaga
     4201 ccccgaagca ggtggtattc ggcgtcattc ccaaaacgtc gaccggcaag ctgcagaaat
     4261 acgtgctgcg tcagcgtgtc ggctcggctg cggcgatcac cgcctgatcg ccgaaaggcg
     4321 tcgcgcttgc ccgatggcgc accgggaacg cgccttgccc tctggagcaa gcgatcagat
     4381 gcggaaggcg gcggaggccg tctccagcgg cgcggcggcg gaagtccgcg ccgttgccgg
     4441 acggtcgatc agcgcatcgg cgaggagggc gccgatccac atcgccgcca gggccaccag
     4501 cgcgccggtc gcgtagatcg cccagccgga cacgccattg cccatcgagc aaccgccggc
     4561 cagaacgccg ccaaagccca tcagcaccgc tccggacaga tagcgcaggc catcgatcgg
     4621 acgatggaac cattcgcgcc tcaggctgcc ggagagcagc gctgccagaa aggcgccgac
     4681 gatgacgccg ggtatcagtc caaaatcgaa atccacacga ccgccgggca gcgacagcgc
     4741 cgaggtggtc gcattcaccg caggcgcgat gaaggacagt ccgcgcaccg gctgcggatc
     4801 gaagctggca gcattcatca ggccggtcag cgcataggtc cccgcaacga tggcgccgat
     4861 ggcaataccc atgatcatgc gctccaggga cagccggcgt cgggcgccca gcaccagtgc
     4921 cgccacgatc aggccgaggc cggacaacag cgcgccgcga gcgccaagtc cggtcgcggc
     4981 tgtcagatcg agcgctcccc ttccttcgac ctgccacagg ccggcgatgg ccgcgcgcgc
     5041 atcgcccagc ggccccttca tcgtcgcata ggccgtggcg acaaacaccg tcgccgtgac
     5101 aatcgagcgg aaattgccgc tggccgagag aaccagcagc cgactaacac agcctcgcga
     5161 caacaccatg ccgattccaa acagcaatcc gccgaccgcc gcgcccgaaa ggctggcccc
     5221 gccattgagc atgcggatcg gatcggccga gatcacgccg gccagcatca ggccctgcac
     5281 gccggcaatg gcggtgccga ccgccagcag ccacagcgcc aacgatggtc cggcatggcc
     5341 ccgcccaact tcgatgacgg ccgagcgcat gcagaagcgg ctgcgctggg ccagaatgcc
     5401 aaaaatcagc ccgatcagcg ctccgctggc gaggccgatc agcggctcac cccagcgttc
     5461 gataattgct tccagcatga caatcctcgt tcgtaattca gaacgaaaat aacaaagttc
     5521 taaaaaacag acaagaccgc ccggaaatta gcaaagaaaa ggcgaaaaat ataacagttt
     5581 gtagaaaaaa ttatcgcaat atgtggctgg gtcaggcaaa acctgctgcc cagcaaagtg
     5641 ggtgcggcaa gaccgcgcgg gcgggaggct acgccatcag gatcgcgcaa tgcccctgga
     5701 ttggcttgcg cgcttcatct gcaatcaccg gccggttgta tagagggcaa tgcgacgtat
     5761 caaaccgcat cccgacaatc cagcgccggg cgcggcaggc gatggcgcgg cgttgccggg
     5821 cttggcctgg ttgccgatca gactccgctc cgcgcattga acgtgtcgca tgcgcggatc
     5881 tggccggtct tcagcccgcg cgtgaaccat tccatgcgct gctgcgagga gccgtgcgtg
     5941 aagctgtccg gcaccgcata gccctgcgat gccttctgca gcttgtcatc gccgatctgg
     6001 ctggcggcat tgagcgcttc ctcgatgtcg ccctgttcaa ggatacggaa tttctgctcg
     6061 gcatgatgcg cccacacgcc ggcaaggcaa tcggccatca gttcgacgcg gacggagagg
     6121 ttgttgctct ccttgcggcc gacgcgctgc tgcatctcct gcaccttggg cagaatgcca
     6181 agttggttct cgacgtgatg gccgacctca tgggcgatga cgtaggcata ggcaaaatcg
     6241 ccgccggcac gcagccgcgt cttcatctcg ccgaagaacg cggtgtcgag gtagaccttg
     6301 gagtcgagcg ggcagtagaa cggccccatc gccgattgcg cgccgccgca gcccgagcgg
     6361 gtgaaattgg agaacatcac cagattgggc ttctggtatt tgatgttgag ttgcttggcg
     6421 agctgctcgg taaacacatc ctcggtgctt ttcagcacgc gggcaacgaa ttgctggtca
     6481 gcatcaggct tggcgccggc gggacgcggg gcggagacct gttcggtgcg gctgccaccg
     6541 gtgacgttcg acagcatctc ggcgccgccg atcaggatcg acggagaaat gccgaaggcc
     6601 caggccacca gcgacaacac gatcatcgtg ccgaagccga gaccgcccga ccgcatcggg
     6661 aaaccgaacc cgccgccgaa cccgccgccg ccatcaccgc gacgatcttc tacattcgtg
     6721 gattcgccaa cgtcgtcgag ccgcatggaa agtctcctca ctccgcttcg gcgatctcta
     6781 acaggtcacc gggcggattt gaatgcgctt tttgggcata gccattccga ccacaacagc
     6841 tatgacgcgc agcagcgaaa tcaagagccc gggggctatt tttgctcaat ttcagcaaat
     6901 cgagccgcca gcgccctgag atcgcgccag gcaaagcgct tctgcgccgg ctgtcgcagc
     6961 agataggctg gatgcaatgt tgccagcgcc gggatggtgc ggtgtccggt gtcatagtcg
     7021 aaccagcggc cgcgcaggcg gaggatgcca tcgcgctgac ccagcagcgt ctgtgccgag
     7081 agagcgccga ggcacaccac cacgacagga tcaaccagcg cgatatgccg ggcgatgaaa
     7141 ggcaggcaga tggcgatttc ctggggcgtc ggcgtgcggt tgcccggcgg acgccacgga
     7201 atgatgttgg tgatgtagac cgaggtccgg tcgagcccga tggccgccag catccggtcg
     7261 agcagcctgc cggaacggcc gacgaatggc aggccctgct catcctcgtc gcggcccggc
     7321 gcctcgccca ccagcatgat ccgcgcctgg ggattgccat cggcaaacac cagctgccgt
     7381 gccgaatgct tgagcgcgca gccatcgaat gcctccatca gcgcgcgcag gtcggcaagg
     7441 cttgcggcgg agtaggcgcg atcgcgggcg ccgcgttcgg cctcg
//
LOCUS       DAOMXG010000121         7410 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_5211_length_7410_cov_10.734058, whole genome shotgun sequence.
ACCESSION   DAOMXG010000121 DAOMXG010000000
VERSION     DAOMXG010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 7410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7410
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5211_length_7410_cov_10.734058"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(277..594)
                     /locus_tag="PLQ11_08890"
     CDS             complement(277..594)
                     /locus_tag="PLQ11_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25053.1"
                     /translation="MRKELVQNVRGGALGAALLVLCGSAGTAQEAVVVGGLTPSVRPA
                     GLPSIQGIHNGIEWYRKALTGVSQPYPASLVWLDRQGEWYTPFTRPGMPGYYDIRGWH
                     GPR"
     gene            complement(1121..3280)
                     /gene="pnp"
                     /locus_tag="PLQ11_08895"
     CDS             complement(1121..3280)
                     /gene="pnp"
                     /locus_tag="PLQ11_08895"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRK25054.1"
                     /translation="MFDIVRQRVNWAGRTLTLETGRIARQADGAVLATYGETTVLATV
                     VSAKEPKPGIDFLPLTVNYQEKAFAAGRIPGGYFKREGRPSERETLVSRLIDRPIRPL
                     MADGYRCDTQIIVTVLSHDLENDPDVVAMVACSAALTLSGAPFMGPIGGARVGCINHE
                     LVLNPTIDQMKESTLDLVVAGTQDAVLMVESEAKELSEETMLKAVMMGHAGFQPVIDA
                     IIKLAEKAAKEPRAFAQPDVSDIEAAVLKIAEKDLRAAYQITTKQDRYNAVDAAKAKV
                     MAEMFPEGAEPKFPKEQVAAVFKELQAKIVRWNILDTRKRIDGRGLEDVRQIVAEVGV
                     LPRTHGSALFTRGETQALVVATLGTGDDEQFIDELEGTRKETFLLHYNFPPFSVGETG
                     RMGSPGRREIGHGKLAWRAVHPVLPAHNEFPYTLRVVSEITESNGSSSMATVCGASLA
                     LMDAGVPVKSPIAGIAMGLILEAERYAVLSDILGDEDHLGDMDFKVAGTANGVTSLQM
                     DIKIAGITEEIMKVALGQAKGGRMHILGEMSKALTGARAELGEHAPRIETLKIPVDKI
                     REVIGSGGKVIREIVEKTGAKVNIEDDGTVKVASADGKSIKAAINWIKSIASDPEVGA
                     IYEGTVVKTTDFGAFVNFFGAKDGLVHISQLANKKVAKTTDVVKEGDKVKVKLMGFDE
                     RGKVRLSMKVVDQATGEDIESKLKAEREAQKGEDKGE"
     gene            complement(3574..3843)
                     /gene="rpsO"
                     /locus_tag="PLQ11_08900"
     CDS             complement(3574..3843)
                     /gene="rpsO"
                     /locus_tag="PLQ11_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HRK25055.1"
                     /translation="MSITAERKSALIAEHATKKGDTGSPEVQVAVLTERITNLTEHFK
                     THVKDNHSRRGLLKLVSQRRRLLDYVKSKDEARYRALIEKLGIRR"
     gene            complement(4002..4967)
                     /gene="truB"
                     /locus_tag="PLQ11_08905"
     CDS             complement(4002..4967)
                     /gene="truB"
                     /locus_tag="PLQ11_08905"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HRK25056.1"
                     /translation="MTDPVSAPPRAPRSPQVKKIRRDVSGWIVLDKPVGLTSTHAVAI
                     AKRLFNAKKAGHAGTLDPLATGLLPLAFGEATKTVPFVMDGEKAYRFTVKWGEQTESD
                     DADGKVVARSDLRPTQEAIEDCLPLFIGEIQQVPPRFSALKIDGERAYDLARDGEMVD
                     LAPRAVVITALRLIESDEETATFEAECGKGTYVRAIARDMGRMLGCLGHVTALRRTRV
                     GPFEEADAVSLDDLRAAGEAGEASDALAACLRPVESALSELAEVRVSRHEAQRLMNGQ
                     AVILRGRDAPVHLGAVYVTEAGRLIALAEADKGQLVPKRAFHLPG"
     gene            complement(4971..5375)
                     /gene="rbfA"
                     /locus_tag="PLQ11_08910"
     CDS             complement(4971..5375)
                     /gene="rbfA"
                     /locus_tag="PLQ11_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020092715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HRK25057.1"
                     /translation="MSKRPESVSGMPSQRQLRVGEQVRHALADILQRGEVHDPVIEGH
                     IITVPEVRMSPDLKLATAYVMPLAGRDEEPVIKTLNRLAKAFRGALARRLREMRSMPE
                     VRFRLDDRFDAASHIDALLNDPRVKRDLNSKD"
     gene            complement(5453..>7410)
                     /gene="infB"
                     /locus_tag="PLQ11_08915"
     CDS             complement(5453..>7410)
                     /gene="infB"
                     /locus_tag="PLQ11_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HRK25058.1"
                     /translation="RPAGPGKTPAPARPERTKAGEAKSRGRLTVQSAFQDDEERQRSV
                     AAFRRRVQRLKGSASEAKEKLAREVIIPETISIQELANRMAERGVDVIRLLMKQGQMH
                     KITDVIDADTAQLVAEEMGHTVKRVAESDVVEGLFDAPDPDETLESRPPVVTIMGHVD
                     HGKTSLLDAIRHANVVAGEAGGITQHIGAYQVTSPLGGKITFIDTPGHAAFTAMRARG
                     ARVTDIVVLVVAADDGVMPQTIEAINHAKAAKVPMIVAINKIDKPEAKAERVRTELLQ
                     HEVQVESFGGETMDVEVSAKQKLNLDKLLEVIQLQAEVLDLKANPNRAAEGSVVEAKL
                     DRGRGPVATVLVQRGTLRQGDIVVAGSEWGRVRALVNDKGEQTGMAGPSVPVEVLGFT
                     GAPEAGDRMAVVENEARAREITAYRVREKREKAALRGAGQAKSLADMMSQIKQAGRKE
                     VPVVVKGDVQGSVEAIVASLDKLSTDEVACRVLHSGVGGITESDITLAQAVGAVVIGF
                     NVRAHKEAREASERNGVEIRYYNIIYDLVDDVKKAMSGLLAPTLRETMLGNATILEVF
                     NITKVGNVAGCQVTDGKVERGAKVRLIRDNVVIHEGTLKTLKRFKDEVKEVQVGQECG
                     MAFENYEDMRKGDVIECFRVEEIQRSL"
BASE COUNT         1364 a         2391 c         2232 g         1423 t
ORIGIN      
        1 tgccgcccat gccggacata cccgacatgc cgcccatgcc gcccatgccg cccataccgg
       61 acatgccttg ggcgaacgcg gcaccggcca gcgccaaggc acccgccatg accgtaccca
      121 tcaaagagct acgcattcgc atgttatcct ccaaagtaac cgtcagcccc tcagggaagt
      181 ctgggaaccg aaatttgaaa gccgggactt cctccggcca atattgaagg cgccctcgcc
      241 ttcaattcga aaaaccaaac ctggcaacca tcccgctcaa cgcggtccgt gccagccgcg
      301 gatgtcataa tatccgggca ttcccgggcg cgtgaacggc gtataccact caccctgacg
      361 atccagccaa accagcgatg ccggataggg ttgcgataca ccagtcagag ccttgcggta
      421 ccattcaata ccgttgtgga ttccctgaat ggacggcagg cccgcaggcc gcaccgacgg
      481 cgtcaatccg ccgacaacaa cagcttcctg ggccgtgcca gccgagccac acagcaccag
      541 cagggcggcc ccgagggccc caccgcgcac gttctggaca agttctttcc gcatgcaaac
      601 acccatccct aggttgtcta cgctatgaag caagcaatag gccaaatttt ggacaatcta
      661 taatcaattg aaataaaaca gatttttatc ttttccacag agtcccactt gccaccactg
      721 ccaattcgac agagacaggc gccgtctgcc cggtgaaggc ttatgcgatt caacgtctta
      781 gataatatat gccaaattgg catataaggg aagcaaatgg agcgctaccg catggcgttc
      841 ggtatggcga acaaatcctt tccgagccct gctcgccccg gcagtgggct ttccgctggc
      901 gataccgcgc tgctggggag cggcgaggac agaaccgtca tttgtctcgg cgcgacagac
      961 aaggggctgg cgttgcgcga ccgacttagt aacgaacgat aacaatcctg cgcttggtgg
     1021 ctctgcgtca aatcgcggct tgacaacaaa aaagggccgg atgcggctcc ggcccctgtt
     1081 ctggcagagc tgccaggatg gcagcgatga atcagcgacc ctactcgccc ttgtcctcgc
     1141 ccttctgcgc ttcgcgttcg gccttgagct tggactcgat gtcctcgccg gtcgcctgat
     1201 cgacaacctt catcgacagg cgcaccttgc cgcgctcatc aaagcccatc agcttcacct
     1261 tgaccttgtc gccttccttg acgacgtcgg tggtcttggc gaccttcttg ttggcgagct
     1321 gggagatatg gacgaggcca tccttggcgc cgaagaagtt gacgaaggcg ccgaagtcgg
     1381 tggtcttgac cacggtgcct tcgtagatcg ctccgacttc cggatcggaa gcgatggatt
     1441 tgatccagtt gatcgctgcc ttgatcgact tgccgtcggc cgaagcaacc ttgacggtgc
     1501 cgtcgtcctc gatgttgacc ttggcgccgg tcttctcaac gatttcgcgg atgaccttgc
     1561 cgcccgagcc aatgacttcg cggatcttgt cgaccgggat cttcagggtc tcgatgcgcg
     1621 gagcgtgctc gccgagttcg gctcgcgcgc cggtcagcgc cttcgacatc tcgccgagga
     1681 tatgcatacg tccgcccttg gcctgaccca gggcgacctt catgatctct tcggtaattc
     1741 cagcgatctt gatatccatc tgcagcgagg tcacgccatt ggccgtgccc gcaaccttga
     1801 agtccatgtc gcccaggtgg tcctcgtcac caagaatatc ggacagcacc gcatagcgct
     1861 ctgcctcgag gatcaagccc atggcgatac cggcaatcgg ggatttcacc ggcacaccgg
     1921 cgtccatcag cgccagcgac gcgccgcaga ccgtcgccat cgaggatgag ccgttcgact
     1981 cggtgatctc cgagaccacg cgcaacgtgt aagggaactc gttgtgagcc ggcagcaccg
     2041 gatgcaccgc acgccaggca agcttgccat ggccgatctc gcgacggcca ggtgaaccca
     2101 tgcgaccggt ctcgcccacc gagaagggcg ggaagttgta gtgtagcaga aaggtctcct
     2161 tgcgggtgcc ttccagttcg tcgatgaact gctcgtcatc gccggtgccg agggtcgcca
     2221 ccaccagcgc ctgggtctcg ccgcgcgtga acagggccga gccgtgggtg cgtggcagga
     2281 cgccgacttc ggcgacgatc tggcggacat cctcaagacc gcggccgtcg atgcgcttgc
     2341 gggtgtcgag gatgttccag cgaacgatct tggcctgcag ctccttgaat acggccgcaa
     2401 cctgctcctt ggggaacttc ggttcggcgc cttccgggaa catctcggcc atcaccttgg
     2461 ccttggccgc atcgaccgca ttgtagcggt cctgcttggt ggtgatctga taggcggcgc
     2521 gcagatcctt ctcggcgatc ttaagcaccg cagcctcgat atccgagaca tccggctggg
     2581 caaaagcgcg cggttccttg gcggccttct ctgccagctt gatgatcgca tcaatcactg
     2641 gctggaaccc ggcatggccc atcatcactg ccttgagcat cgtctcttcc gacagctcct
     2701 tggcctccga ttcgaccatc agaaccgcat cctgcgtgcc ggcgaccacc agatcgaggg
     2761 tcgattcctt catctgatca atggtcgggt tgagcaccag ttcgtggttg atgcagccga
     2821 cacgcgcgcc accgatcggg cccatgaacg gggcgcccga cagggtcagc gccgccgaac
     2881 aggcgaccat tgcgacgaca tctggatcgt tctcgaggtc gtggctgagt acggtgacga
     2941 tgatctgggt gtcacagcga tagccatcgg ccatcaatgg acggatcgga cggtcgataa
     3001 ggcgcgagac caacgtctcg cgttcggacg ggcggccctc acgcttgaaa taaccgccgg
     3061 ggatgcggcc ggccgcgaac gccttttcct ggtagttcac ggtgagcggc aaaaagtcga
     3121 tgccgggctt gggttccttg gccgagacga cggttgcgag cacggtggtc tcgccatagg
     3181 tggcgagcac ggcgccgtcg gcctgccggg cgatgcggcc ggtttcgagg gtgagcgtgc
     3241 ggcccgccca gttcacgcgc tggcgaacga tatcaaacat gggttcttgc tttctggcta
     3301 tgtgtgtcga acgaaaccat gggcaagacg acgggcggct ttggaacaac acgcggcact
     3361 ggccgcacaa gccgtcggca atcctgccat ggcacggaat ctacgcaacg ggcgggagac
     3421 cggagccacc cgccgcaact gaatggccgg gatacccccg gcctgtcgcc tcagagcgct
     3481 ctcagtgaat tcacagtcat tgaaagtgct ctagtttctt gttttgtcgc attttcttaa
     3541 cgcgaaccgg aatccacttc gcttgaaaat gctctagcga cggatgccga gcttctcgat
     3601 cagcgcccga taccgcgcct cgtccttgga cttgacgtag tccagcaggc ggcggcgctg
     3661 ggagacgagc ttgagcaggc cacgccggga atgattgtcc ttgacgtggg tcttgaagtg
     3721 ctcggtgaga ttggtgatgc gttcggtcaa aaccgcgacc tggacttccg gcgaaccggt
     3781 gtcacccttc ttggtggcgt gttcggcgat cagcgcggac ttgcgctcgg cagtaatcga
     3841 catcggatgt tccttgttgt tggaggatgg ccgggccggg atgtcgtcca gcgcggtcgg
     3901 gacagcgcgg gaccccccac gctggcgcgg cttatgcacg aaaaggcgag gaaagggaag
     3961 agggctcgcc gcctcggtgg acggatgagc ccttcaaggc ctcacccagg cagatgaaac
     4021 gcccgtttgg gcaccagctg acccttgtcc gcctcggcga gcgcgatcag ccggcccgcc
     4081 tcggtgacat agaccgcccc gaggtgaacc ggcgcgtcgc ggccacgcag gatcaccgcc
     4141 tggccgttca tcagccgctg cgcctcgtgg cgcgagacgc gcacctctgc cagctcgctc
     4201 agagcagatt ccaccggacg caggcaggcg gcgagcgcat ccgaggcttc acctgcctcg
     4261 cctgcggcac gcagatcatc aagcgagacc gcatcagcct cctcgaaggg gccaacgcgg
     4321 gtgcggcgca gcgcagtcac atggccgagg cagccgagca ttcggcccat gtcgcgggcg
     4381 atggcgcgca cataggttcc cttgccgcat tcggcctcga acgtcgcggt ctcctcgtca
     4441 gactcgatca gacgcagcgc ggtaatgacg accgcgcgcg gcgccagatc gaccatctcg
     4501 ccgtcacggg cgagatcata ggcacgctcg ccgtcgatct tcagcgcgga aaaacgcggc
     4561 ggaacctgct ggatctcgcc aataaaaagg ggcaggcaat cctcgatggc ctcctgcgtc
     4621 ggtcgcaggt cggaccgggc gacgacttta ccgtcggcat cgtcggattc ggtttgctcg
     4681 ccccatttga cggtgaagcg ataggctttt tcgccgtcca tcacgaaagg tacggttttg
     4741 gtcgcctcac cgaaggcaag cggcagcaga ccggttgcca gcggatcgag cgtgccggca
     4801 tgcccagcct tcttggcgtt gaacagccgc ttggcaatcg ccaccgcatg ggtcgaggtc
     4861 agcccgaccg gcttgtcgag gacgatccag cccgacacat cgcggcgaat tttcttgacc
     4921 tgcggcgaac gtggagcgcg gggcggagcg gaaacagggt cggtcatgcg tcagtcttta
     4981 ctgttgaggt cacgcttgac acgcggatca ttcagcagcg catcgatgtg gctggcggcg
     5041 tcgaagcgat catccaaccg gaagcggacc tccggcatcg agcgcatctc gcgcagacgt
     5101 cgcgcaagtg cgccgcgaaa cgccttggcc aagcggttca gggtcttgat caccggttcc
     5161 tcatcgcggc cggccagcgg catgacatag gcggtggcaa gtttcagatc ggggctcatc
     5221 cgcacttccg gcacggtgat gatatgcccc tcgatcaccg gatcatgcac ttcgccgcgc
     5281 tggagaatat ccgccaatgc atggcgcacc tgttcgccga cgcgcagctg gcgctgggat
     5341 ggcattccgg aaacggattc tggacgtttt gacattgttc aacaaccctg ccgccgggat
     5401 cggaccggtc gtcggtaaag ggtcccggcg gctgatcacc gggaccattt ggtcagagcg
     5461 agcgctggat ttcctcgacg cggaagcact cgatgacatc acccttgcgc atatcctcgt
     5521 agttttcgaa ggccatgccg cattcctggc ccacctgcac ttccttgacc tcgtccttga
     5581 agcgcttgag cgtcttgagc gtgccttcgt ggatgaccac gttgtcgcga atgaggcgga
     5641 ccttggcgcc gcgctcgacc ttgccatcgg tgacctggca accggccaca ttgcccacct
     5701 tggtgatgtt gaacacctcg agaatggtgg cgttgcccag catcgtctcg cgcagggtcg
     5761 gcgccagcag gcccgacatc gctttcttca cgtcatccac aaggtcatag atgatgttgt
     5821 agtatcggat ctcgacgccg ttgcgctcgg atgcctcgcg cgcttccttg tgggcgcgga
     5881 cattgaagcc gatcaccacc gcaccgaccg cctgagccag cgtgatatcc gattccgtga
     5941 tgccgccgac acccgaatgc agaacgcggc aggccacctc atcggtggag agcttgtcca
     6001 gcgaggcaac gatcgcctcg accgagccct gaacgtcgcc cttgacgacc acgggaactt
     6061 ccttgcggcc ggcctgcttg atctgactca tcatatcggc cagcgacttg gcctggccgg
     6121 cgccgcgcaa ggccgccttc tcgcgctttt cgcggacccg gtaagcggtg atctcgcggg
     6181 cgcgcgcctc gttttcaacg acagccatgc ggtcgcccgc ttccggcgca ccggtaaagc
     6241 caaggacctc gaccgggacc gaaggacctg ccatgcctgt ctgctcgccc ttgtcattga
     6301 ccagagcccg cacgcgaccc cattccgagc cggcaacaac gatgtcaccc tgcctgagcg
     6361 tgccgcgctg caccagcacg gtcgcaaccg gaccgcgacc acgatccagc ttggcttcga
     6421 caaccgaacc ctctgcggcc cggttcggat tggccttgag gtcgagcacc tcggcctgaa
     6481 gctggatgac ttcgagaagc ttgtcgagat tgagcttctg cttggcagaa acctcaacgt
     6541 ccatggtctc gccgccgaag gattcgacct gcacctcatg ctgcagcaac tcggtgcgca
     6601 cccgctcggc cttggcctcg ggcttgtcga tcttgttgat cgccacgatc atcggaacct
     6661 tggcagcctt ggcgtgattg atcgcctcga tcgtctgagg catcacgccg tcatcggcgg
     6721 ccaccacaag aaccacaata tcggtcacgc gggcgccgcg ggcgcgcatc gccgtgaagg
     6781 cggcgtggcc cggtgtatcg atgaaggtaa tcttgccgcc aagcggcgag gtgacctgat
     6841 aagcgccgat gtgttgcgta atcccgccag cttcgcccgc cacaacattg gcatggcgga
     6901 tcgcatcgag caacgaggtc ttgccatggt cgacatggcc catgatggtg acgaccggcg
     6961 ggcgggactc cagcgtttcg tcgggatccg gcgcatcgaa aagcccttcg accacgtccg
     7021 attcggcaac acgcttgaca gtatggccca tttcctcggc caccagctgc gccgtgtcgg
     7081 catcgatcac atcggtgatc ttgtgcatct ggccctgttt catcagcagc cggatgacat
     7141 cgacgccgcg ctctgccatg cggttggcga gttcctgaat gctgatggtt tccggaatga
     7201 tcacttcccg tgccagcttt tccttggctt cggacgcgct acccttcagg cgctgcacac
     7261 gtcggcggaa cgcggcaacc gagcgctgac gctcctcgtc gtcctgaaag gcactctgca
     7321 ccgtcaggcg gccgcgggat tttgcttccc cggccttggt ccgctccgga cgggcggggg
     7381 ccggtgtctt gcccggcccg gccggacgcc
//
LOCUS       DAOMXG010000122         7364 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_5251_length_7364_cov_10.648789, whole genome shotgun sequence.
ACCESSION   DAOMXG010000122 DAOMXG010000000
VERSION     DAOMXG010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 7364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7364
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5251_length_7364_cov_10.648789"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="PLQ11_08920"
     CDS             <1..997
                     /locus_tag="PLQ11_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015857645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRK25059.1"
                     /translation="SGFGDWSRRRLAEVEAALAAKGQMLPFEPLDHRILLGSKGTPTI
                     GAVAAGNLSGPRRISAGAARDSLIGVRFVNGRGEAIKSGGRVMKNVTGLDLVKLQCGA
                     HGTLGPLTEVIFKVLPAPETVRTLVLQGLDDKRAVAALATAMGTPFEVSGSAHLPAGD
                     PREKSRTVIRLEGFADSLAYRADALAKVLKPFGASTVLDEAESRPLWQTIRDAAPLAS
                     GAAHAIWRLSVKPTAGPDVVAAIRAAVPLVGWFYDWSGGLVWLAAPATGDGGATAVRA
                     AVTEAGGHATLVKAPADLRTAVPVFQPEALAVARLAEGIRRSIDPDGLFNPGLMG"
     gene            1009..2331
                     /gene="glcF"
                     /locus_tag="PLQ11_08925"
     CDS             1009..2331
                     /gene="glcF"
                     /locus_tag="PLQ11_08925"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HRK25060.1"
                     /translation="MQTSFTLQQLADSAMQTSEKILRTCVHCGFCTATCPTYLLLGDE
                     LDSPRGRIYLIKDMFENGRPATGEIVQHLDRCLSCLSCMTTCPSGVHYMHLIDHARVH
                     VENTYRRPLADRLMRNTLAAILPYPARFRLSLIGAKLGRPFAPLLGRLPVIGKRMQAM
                     LELAPKGSVGRTALEGPGTHPAAERKGRVILMQGCAQPVLAPSINEATIRVLNRHGIE
                     VVIPKGEGCCGALVHHMGKEHESHDFARRQIDAWITEIDARGADAILITASGCGTTVK
                     DYGFMFRDDPAYATKAARVTALARDVSEYLATLDLKRRPGPGLTVAYHSACSMQHGQK
                     ITDIPKKLLTAAGFKVKDVPEGHICCGSAGVYNILQPEIAGALRERKVDNIARTWPDV
                     IATGNIGCMTQIGKGLSDRGDTTPIVHTVELIDWATGGPKPPALERAV"
     gene            complement(2354..3772)
                     /locus_tag="PLQ11_08930"
     CDS             complement(2354..3772)
                     /locus_tag="PLQ11_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HRK25061.1"
                     /translation="MRGMVAGALAATGGLLMSGMPALAAEGLDGKALGLAWSIPFAGL
                     LLSMALCPLFLHRTWEHHYGKIAAGWALLTLIPMAMSQGAGLTMEAFLHAMLIEYMPF
                     IILLFTLYTVAGGILVSGNLHGTPLVNASLLTIGAVLASFVGTTGASMIMIRPVVRAN
                     DNRRVNVHVIIFFIFLVSNVGGSLTPLGDPPLFVGFLKGVDFFWTLQALWKPTLITFA
                     ILMAMFIAIDTFLYWTEETLPTLPDKTPDRAIRIYGQINILLLAGVIAAILMSAWWKP
                     GVTLKLPGAPLPLEGVLRDVILLFIAWLSMKLTPKQVRAGNEFSWGAITEVGKLFAAI
                     FVCVIPVIAILKAGMNGALAPLVSLVTNADGSANVQMYYFMSGLLSSFLDNAPTYLVF
                     FELAGGNAAEMMTTKATILKAISLGAVYMGANTYIGNAPNLMVATIAKDMGIKMPSFI
                     GYMSWSCVLLVPVLFFVGFYLL"
     gene            3967..4515
                     /locus_tag="PLQ11_08935"
     CDS             3967..4515
                     /locus_tag="PLQ11_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25062.1"
                     /translation="MTNKLAAVAVLGLAMLAGPAQAQQKAPPKAGAAAPAQPEQPTSG
                     PISLIDMKVQMFLERSGTWSENLVNTRKKLHNTVLGQGDAGEPADAVLVTLVFEGTKN
                     TRGSDKLARDLAQVKVTQATKAGNKILLNRVYGGFLFGDNGLAHKAFVIDNATCLPLE
                     IEAKIGRTRKAAKIEFTCDPKS"
     gene            4595..5383
                     /locus_tag="PLQ11_08940"
     CDS             4595..5383
                     /locus_tag="PLQ11_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009762992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HRK25063.1"
                     /translation="MCRWIAYRGKPVFIEDVVVKPGHSLVDQSLHASEGKTQTNGDGF
                     GLGWYGEREEPGLYREIRPAWSDENLKAICAQVRSPLFFAHVRAATGTATTRANCHPY
                     AHGRWLFMHNGQIGGYAGLRRRIEGLIPDEYYLGRQGSTDSEAIFLSALARMDRLDPA
                     TAVVSVLADVKQMMQTASVCAPLRFTSALTDGRDVYAFRWACDDKAPTLYWRFSDGDL
                     CVVSEPYDSERGCWNPIPQGSMLIARARQTVEIVPMCIEARRAA"
     gene            complement(5451..>7364)
                     /gene="polA"
                     /locus_tag="PLQ11_08945"
     CDS             complement(5451..>7364)
                     /gene="polA"
                     /locus_tag="PLQ11_08945"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HRK25064.1"
                     /translation="AGLTPQALAEARAAAAPAVPFDLDRYETVTDLARLNAWIAEARA
                     AGLVAFDTETTSLDPMQAELAGFSLAVRPGVACYVPLNHRASGGGDLFGGGERVAGQI
                     NEREALAVLKPLLTDAGVLKIGQNLKFDALVLMQRGLAITPADDTMLLSYVLDAGRGG
                     HGMDDLSERHLGHRPVSFDSVTGTGKSRITFDRVALEAATRYAAEDADVTLRLWQLLK
                     PRLAAEGMTNVYETLERGMVGVLARMEQRGIAIDRQILSRLSGDFAQGMARLEADIHA
                     LAGENFNIASPKQLGDILFGKLGLPAPKKTATGALSTGAQVLEDLAAEGHDLPRRILD
                     WRQLSKLKSTYTDALPGYVNPATQRVHTSFALAATTTGRLSSSEPNIQNIPVRTEEGR
                     KIRTAFIATPGHKLISADYSQIELRLLAHIAEIPQLRQAFAEGVDIHAMTASEMFGVP
                     VAGMPSEVRRRAKAINFGIIYGISAFGLANQLGIGREEAGAYIKKYFERFPGIREYME
                     ETKATARRQGFVTTIFGRKCHYPNINAKNAAERAFNERAAINAPIQGSAADIIRRAML
                     RMEDALAAAKSGARMLLQVHDELVFEVAEGEVEAAIPVIRRVMVEAPLPAIQLKVPLQ
                     VDARAARNWDEAH"
BASE COUNT         1313 a         2383 c         2423 g         1245 t
ORIGIN      
        1 cagcggtttc ggtgactggt cgcgccggcg gttggccgaa gtcgaggcgg cgctggcagc
       61 caaaggccag atgctgccgt tcgagccgct cgatcatcgc atcctgctgg ggtcgaaagg
      121 aacgccgacc atcggcgccg tcgcggcggg taatctttcc ggcccgcgcc gcatatctgc
      181 gggtgcagcc cgcgacagcc tgatcggcgt gcgtttcgtc aatggcaggg gcgaggcgat
      241 caagtccggc ggccgggtga tgaagaacgt caccggcctc gacctggtca agctgcaatg
      301 cggcgcccat ggcacgctcg gcccgctgac cgaggtgatc ttcaaggttc tgccggcgcc
      361 ggaaacggtg cggacgctgg tgctgcaagg tctggatgac aagcgtgcgg tggcggcgct
      421 ggcgacggcg atgggcactc cgttcgaggt ctcgggctcg gcgcatctgc ctgccggcga
      481 tccgcgtgag aagtcgcgga cggtgatccg gctcgaaggc tttgccgatt cgctcgccta
      541 ccgcgccgat gcgctggcaa aagtgctgaa accgtttggt gcatccacgg ttctggacga
      601 ggccgagagt cgccccctgt ggcaaaccat ccgtgatgcc gctccgctgg cctccggcgc
      661 cgctcatgcg atctggcggc tgtcggtcaa gccgacggcg gggccggatg tcgtggccgc
      721 gattcgggct gccgttccgc tcgtgggctg gttctatgac tggtcgggcg gactcgtctg
      781 gctggcggcg ccggcaacgg gcgatggcgg ggcgacagca gtgcgcgcgg cggtcactga
      841 ggctgggggg catgcaaccc tggtcaaggc gccggctgat ctgcgcaccg ctgttccggt
      901 gttccagccc gaggcgctgg cagttgcgcg ccttgccgag ggcattcgcc gcagcatcga
      961 cccggacggg ttgttcaacc ccggattgat gggctgagag taggatccat gcaaaccagt
     1021 ttcaccctcc agcagcttgc cgacagcgcg atgcagacct cggaaaagat cctgaggacc
     1081 tgtgtgcatt gcggcttctg caccgccacc tgcccgacct atctcctgct cggcgatgaa
     1141 ctcgattccc cgcgcggccg catctatctg atcaaggaca tgttcgagaa cggccgcccg
     1201 gcgaccggcg agatcgtcca gcatctcgac cgctgcctct cgtgcctgtc gtgcatgacc
     1261 acctgcccgt cgggcgtgca ttacatgcat ctgatcgatc atgcgcgcgt gcatgtcgag
     1321 aacacctatc ggcgcccgct ggccgatcgg ctgatgcgca atacgctggc ggcaatcctg
     1381 ccctatccgg cgcggttccg cctgtcgctg attggcgcca agctgggccg gccctttgcg
     1441 ccgctcctcg ggcggttgcc ggtgatcggc aaacgcatgc aggcgatgct cgaactggcg
     1501 ccgaaaggca gcgtcggccg caccgcgctg gaagggccgg gcacccaccc cgcagccgaa
     1561 cgcaagggcc gcgtcatcct catgcagggc tgcgcccagc cggtgctggc gccgtcgatc
     1621 aacgaggcga cgatccgcgt gctcaaccgc cacggcatcg aggtggtgat cccgaagggc
     1681 gaggggtgtt gcggcgccct ggtccatcac atgggcaagg agcacgaaag ccacgatttc
     1741 gcccgccgcc agatcgacgc ctggatcacc gagatcgatg cgcgcggcgc cgatgccatc
     1801 ctcatcaccg cctcgggctg cggcacgacg gtgaaggatt acggcttcat gttccgtgac
     1861 gatccggctt atgccacaaa ggcggcgcgc gtcaccgcgc tggcgaggga cgtcagcgaa
     1921 tatctcgcga cgctggacct gaagcgccgt cccggcccgg gactgactgt ggcctatcat
     1981 tccgcctgct cgatgcagca cggccagaag atcacggaca ttcccaagaa gctgctgacg
     2041 gcggccggct tcaaggtcaa ggatgtgccg gaggggcata tctgctgcgg ctccgccggg
     2101 gtctacaaca tcctgcaacc cgaaatcgct ggcgccctgc gcgagcgcaa ggtggacaac
     2161 atcgcccgca cctggcccga tgtcatcgcc accggcaaca tcggctgcat gacccagatc
     2221 ggcaagggcc tgtcggatcg tggcgatacg acgccgattg tccacaccgt cgagctgatt
     2281 gactgggcca ccggcgggcc gaagccgccg gcgctggaac gggcggtctg accgcccgcc
     2341 cctcccacca gcgctacagc agatagaacc cgacgaagaa cagcaccggc accagcagca
     2401 cgcaggacca gctcatgtag ccgatgaagc tcggcatctt gatgcccatg tccttggcga
     2461 tggtggcgac catcaggttc ggtgcgttgc cgatgtaggt attggcgccc atgtagaccg
     2521 cgccgagcga gattgccttc aggatcgttg ccttggtggt catcatctcg gctgcattgc
     2581 cgccggcgag ttcgaagaac accagatagg tcggcgcatt gtcgaggaag gacgagagca
     2641 ggcccgacat gaaataatac atctgcacat tggcgctgcc gtcggcgttg gtgaccagcg
     2701 agaccagcgg cgccagcgcg ccgttcatgc ccgccttgag gatggcgatc accgggatga
     2761 cgcagacgaa gatcgccgcg aacagcttgc cgacctcggt gatcgcgccc catgagaatt
     2821 cgttgccggc gcgaacctgc ttgggtgtca gtttcatcga cagccaggcg atgaagagca
     2881 ggatgacgtc gcgcaacacg ccttccagcg gcagcggcgc ccccggcagc ttgagggtga
     2941 cccccggttt ccaccaagcc gacatcagga tggcggcgat caccccggcg agcagcagga
     3001 tgttgatctg tccatagatg cggatggcgc ggtccggcgt cttgtccggc agcgtcggca
     3061 gcgtttcttc ggtccagtag aggaaagtgt cgatggcaat gaacatcgcc atcaggatgg
     3121 cgaaggtgat cagcgtcggc ttccacaatg cctgcagcgt ccagaagaaa tcgacaccct
     3181 tgaggaagcc gacgaacagc ggcggatcgc ccagcggtgt caggctgccg ccgacgttgg
     3241 acaccaggaa gatgaagaag atgatcacat gcacattgac gcgccggttg tcattggcgc
     3301 gcaccaccgg ccggatcatg atcatcgagg cgccggtcgt gccgacaaag ctcgccagca
     3361 ccgcgccgat agtcagaagg ctggcattga cgagcggcgt gccatgcagg ttaccgctga
     3421 ccagaatgcc gcctgccacg gtatagaggg taaacagcag gatgatgaac ggcatgtatt
     3481 cgatcagcat cgcatgcagg aaggcttcca tcgtcagccc ggcgccctgg ctcattgcca
     3541 taggaatcag cgtcagcagc gcccagccgg ccgcgatctt gccgtagtga tgttcccagg
     3601 tgcgatgcag gaacagcgga cacagcgcca tcgacagcag cagtccggca aagggaatcg
     3661 accaggccag cccgagcgcc ttcccgtcca gcccctcggc ggccagggcc ggcatgcccg
     3721 acatcaacag cccgcccgtg gcggcaagcg cgcccgcaac cattccccgc atggttcctc
     3781 cccttattgc accgacttta ggggctcatg tccaacggtc aagccggcaa acagtgccga
     3841 taattgctca ggcagagaaa aagacagcgg gcgttcatct tgtcgttgct actcggcgcc
     3901 ctgtagaaga ggcattggac aggtcggcgg gaaatcgcca gtcggaaagg caatggggga
     3961 ctatccatga cgaacaaact ggcggcggtt gcagtgctcg gtctggcaat gctggcaggg
     4021 cctgcccagg cgcagcagaa agcgccgccg aaggccggcg ctgcggcccc ggcccagccg
     4081 gaacagccga cctccggccc gatctcgctg atcgacatga aggtgcagat gtttctggag
     4141 cgctccggaa cctggtcgga aaacctcgtc aataccagga agaagctgca caacacggtg
     4201 ctcggccagg gcgatgccgg tgagccggcc gacgccgtgc tggtgacgct ggtgttcgag
     4261 ggcaccaaga acaccagggg atcagacaag ctcgcgcgcg atcttgcgca ggtgaaggtg
     4321 acccaggcca ccaaggccgg caacaagatc ctgctcaatc gcgtctatgg cggtttcctg
     4381 ttcggcgata acggccttgc ccacaaggcc ttcgtcatcg acaatgccac ctgcctgccg
     4441 ctggagatcg aggccaagat cggcaggacc cgcaaggcgg caaagatcga attcacctgc
     4501 gatccgaaat cctgacatgg agccgggcgc gacgctttgc ctgcgcccga cttcgcggta
     4561 accttggcga aggattcaac cggacgcttc gcccatgtgt cgctggatcg cctatcgcgg
     4621 caaacccgtg ttcatcgagg atgtggtggt caaacccggc cattcgctgg ttgaccagtc
     4681 cctgcacgcc tcggagggca agacccagac caacggcgac ggtttcggcc tcggctggta
     4741 tggcgagcgc gaggagccgg ggctttaccg cgaaatccgc ccggcctggt cggatgagaa
     4801 cctcaaagcc atctgcgcgc aggtgcgctc gccactgttc ttcgcccatg tccgcgccgc
     4861 caccggaacc gcgaccacgc gcgccaattg tcatccctat gcccatggcc gatggctgtt
     4921 catgcacaac ggccagatcg gcggctatgc cggcctcagg cgccgcatcg aaggcctgat
     4981 ccccgacgaa tactatctcg gtcgccaggg ctctaccgat tcagaagcga ttttcctgtc
     5041 ggcactggcg cgcatggacc ggctcgatcc ggcgacggcg gtggtctcgg tgcttgccga
     5101 cgtcaagcag atgatgcaga cggccagcgt gtgcgcgccg ctgcgtttca cctcggcact
     5161 gaccgatggc cgcgatgtct atgccttccg ctgggcctgc gacgacaagg cgccgacgct
     5221 ctactggcgc ttcagcgacg gcgatctctg cgtcgtctcc gaaccctatg acagcgagcg
     5281 cggctgctgg aacccgatcc cgcagggctc gatgctgatc gcgcgggcaa ggcagactgt
     5341 cgagatcgtg ccgatgtgca tcgaggcaag acgggcggcg tagcgcttgc cctcgtcgtt
     5401 gccaggaggg caaagcccga cgcggcaatc cagaccgacg ttccaatccc tcaatgcgcc
     5461 tcgtcccagt tgcgggcggc ccgcgcatcg acctgcagcg gcactttcag ctgaattgcc
     5521 ggaaggggcg cttccaccat cacgcggcgg atgaccggga tcgccgcctc cacctcgccc
     5581 tcggccacct cgaacaccag ttcgtcatgc acctgcaaca gcatccgtgc gccggatttc
     5641 gcggcggcga gcgcatcttc catccgcagc atggcgcggc ggatgatgtc ggcggcggaa
     5701 ccctggatcg gcgcgttgat cgcggcgcgc tcgttgaagg cgcgttcggc ggcgttcttg
     5761 gcgttgatgt tgggatagtg gcatttgcgg ccgaagatgg tggtgacgaa tccctgccgc
     5821 cgcgcggtcg ccttggtctc ttccatatac tcgcggatgc cggggaagcg ctcgaaatac
     5881 ttcttgatat aggcgccggc ttcctcgcgc ccgatgccaa gctggttggc gaggccaaag
     5941 gcggaaatgc cgtagatgat gccgaaattg atggccttgg cccgccgccg cacctcggag
     6001 ggcatgccgg ccaccggcac gccgaacatc tcgctcgcag tcatcgcatg gatgtcgacg
     6061 ccctcggcaa aggcctgccg gagttgcgga atctcggcga tatgcgcgag cagccgcaat
     6121 tcgatctggc tgtaatcggc agagatcagc ttgtggccgg gtgtggcgat gaaggcggtg
     6181 cggatcttgc ggccttcctc ggtgcgcacc ggaatgttct ggatgttcgg ctctgatgac
     6241 gacagccgcc ctgtggtcgt cgcggccagc gcgaacgagg tatgcacccg ctgggttgcc
     6301 gggttcacat agccgggcag ggcatcggta taggtcgatt tgagcttgga aagctggcgc
     6361 cagtcgagaa tgcggcgggg caggtcatgg ccttcggcgg cgagatcctc cagcacctgc
     6421 gcgccggttg acaacgcgcc ggtcgcggtt ttcttcggcg ccggcaggcc gagcttgccg
     6481 aacaggatat cgcccagttg ctttggcgag gcgatattga agttttcccc ggcgagcgca
     6541 tggatgtccg cttccagccg cgccatgccc tgcgcgaagt cgccggacag ccgcgacagg
     6601 atctgcctgt cgatggcaat gccgcgctgc tccatgcggg ccagcacgcc gaccatgccg
     6661 cgctccagcg tctcgtagac attggtcatg ccctcggcgg caaggcgcgg cttcagcagc
     6721 tgccacagcc gcagcgtcac atcggcatcc tcggcggcgt agcgggtggc tgcctccagc
     6781 gctacgcggt cgaaggtgat gcggctcttg ccggtgccgg tgaccgaatc gaagctgacc
     6841 ggcctgtggc cgagatgccg ctcggagaga tcgtccatgc catgcccgcc gcgcccggcg
     6901 tcgagcacat aggacaagag catggtgtcg tcggcagggg tgatcgcaag gccgcgctgc
     6961 atcagcacca gcgcatcgaa cttcagattc tggccgatct tgagcacgcc cgcatcggtc
     7021 agcaacggct tgagcacggc cagcgcctcc cgctcgttga tttgcccggc cacccgttcg
     7081 ccgccgccga acagatcgcc gccgccgctc gcccgatggt tgagcggcac atagcaggcg
     7141 acacccggcc gcaccgccag cgaaaagccg gcaagctcgg cctgcatcgg atcgagcgag
     7201 gtggtctcgg tgtcgaaggc taccaatcct gcggcgcggg cctcggcaat ccaggcgttc
     7261 agcctggcga ggtcggtaac ggtctcgtag cggtcgagat cgaacggcac ggcaggagcg
     7321 gcggccgcgc gcgcttcggc cagcgcttgc ggtgtcaggc cagc
//
LOCUS       DAOMXG010000123         7265 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_5362_length_7265_cov_11.504161, whole genome shotgun sequence.
ACCESSION   DAOMXG010000123 DAOMXG010000000
VERSION     DAOMXG010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 7265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7265
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5362_length_7265_cov_11.504161"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(440..1009)
                     /locus_tag="PLQ11_08950"
     CDS             complement(440..1009)
                     /locus_tag="PLQ11_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25065.1"
                     /translation="MVAVLKRWLLMGLVAAAAGVALLVIDPTAALLFRVVGLVGMALF
                     ALSIVVMLLTFRRARTLSAWALLFSALSSILCTGLMARFGGVSLATAMMVLSAVAGGL
                     VGIAWALTNLLFVENETVRARGTLWFLAIWALTLLVPQAVALAGARTPQAMMILSFFG
                     MGLALGNSLSLLVRRSLALRLPMARGGSG"
     gene            complement(1149..1829)
                     /gene="purQ"
                     /locus_tag="PLQ11_08955"
     CDS             complement(1149..1829)
                     /gene="purQ"
                     /locus_tag="PLQ11_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003604527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HRK25066.1"
                     /translation="MHAAVITFPGSNREGDVARALKLGGARVSHAWHADYELPRGTDL
                     VVLPGGFSYGDYLRCGAIAGRAHIMDAVRKHAAGGGLVLGICNGFQILCESGLLPGIL
                     TRNAGLKFLCKMQHLKVGRTDTPFTRLYRRDQVIEVCIAHGEGNYTADAETLKRLEGE
                     DRVAFRYCDATGDTNEAANINGSLNHIAGIYSDRRNVLGMMPHPENLIEGLTGGTDGL
                     ALFKGLAA"
     gene            complement(1829..2068)
                     /gene="purS"
                     /locus_tag="PLQ11_08960"
     CDS             complement(1829..2068)
                     /gene="purS"
                     /locus_tag="PLQ11_08960"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HRK25067.1"
                     /translation="MKARVTVTLKNGVLDPQGKAIEGALKSLGVEGVASVRQGKVFDI
                     EVKAADKAAAEAILRQSAEKLLANTVIENFKVEVL"
     gene            complement(2126..2767)
                     /gene="metW"
                     /locus_tag="PLQ11_08965"
     CDS             complement(2126..2767)
                     /gene="metW"
                     /locus_tag="PLQ11_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HRK25068.1"
                     /translation="MTDAELRDLSSATQARVDHLVVAEMVTPGARVLDVGCGDGALLS
                     LLVRMRQVDGRGIELSREGVATCVSHGHAVIQGDADTDLGDYPDDCFDFVILSQTLQA
                     TRNPRRVIEQMLRIGHHAIVSFPNFGHWGVRSSLFFKGRMPVTANLRHAWYDTPNIHF
                     CTIRDFVALVEEIPATMEKAVALNAAGERIGFNAPWWLWNLLGEQAVFLIKRV"
     gene            complement(2767..3939)
                     /locus_tag="PLQ11_08970"
     CDS             complement(2767..3939)
                     /locus_tag="PLQ11_08970"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HRK25069.1"
                     /translation="MARKSRQAAGSAHSQAESPDSAVIRFDMDRPLQLESGQFLAPLQ
                     VAYNTYGTLNAARSNAILICHALTGDQHVANVHPVTGKPGWWEIMIGPGRLMDTNRYF
                     VICANVLGACMGTTGPSSINPATGRPFGLDFPVITIGDMVNAQAMLIEHLGIDKLLSV
                     VGGSMGGMQVLDWAARYPERVFSAMPIATGAKHSAQNIAFDEVGRQAVMADPDWRGGR
                     YIEEGTRPEKGLAVARMAAHITYLSEQALQRKFGRALQDRDSRTFSFDADFQVESYLR
                     HQGSSFVERFDANSYLYLTRAMNYFDMAADHGGRLANAFAGSKTRFCVISFTSDWLFP
                     TSDSRAIVHALNAAGAPVSFVEIESDKGHDAFLLDEPALFATMRGFLEAAAAARGV"
     gene            4148..5014
                     /locus_tag="PLQ11_08975"
     CDS             4148..5014
                     /locus_tag="PLQ11_08975"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HRK25070.1"
                     /translation="MATPKEPGLHELRLELDRIDAAMQALLIERSEITDKVEQVKRRA
                     GTAGSAFRPGREAEIMRMLAERHRGSYPFDGTENIWRVIIATSTYTQVPYQVHGDVSG
                     GDAPMRDSVRFHFGFTVPFLPHPDASTVIEAVAARPSDLGVFPRSQSMETGAWWRKLH
                     GGDRPKIIARLPFVERENHPSGIPVFVIARPLKEAAVRDEIVASVTVERWSPRIAQAL
                     GPLAARIEATVGIDSGLSLLVTHPGLVAAEAVIAALRNAGMEARYEEVGSHAAAFAAD
                     LRKAGDRGPASA"
     gene            5164..6273
                     /gene="hisC"
                     /locus_tag="PLQ11_08980"
     CDS             5164..6273
                     /gene="hisC"
                     /locus_tag="PLQ11_08980"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HRK25071.1"
                     /translation="MPKSSAPRPIPRAGVMEIDAYVPGKSAAPPGVKLYKLSSNETPL
                     GASAKAISAYKDIAGKLEFYPDGNSTALREAIARRYGLDASRIVCGAGSDELLNLITH
                     AFIGPGDEGIFTEHGFLVYRIAITAAGGRPVVVKERQFTADVDAILAAVTARTKVVFL
                     ANPNNPTGTYLPFSEVKRLHEGLRGDIVLVLDAAYAEYVRRNDYASGLELVSESDNVV
                     MTRTFSKIHGLAGLRIGWAYMPAGIADALNRIRGPFNLSAPAIAAGAAAIDDSAHMEA
                     AAAHNETWNAWLTKELAGLGLSVTPSVGNFLLIHFPRTAGQTARQADQFLSERGLILR
                     AVGAYGLPDALRMTIGSEEANRKVVAALRDFLGKA"
     gene            6281..7213
                     /locus_tag="PLQ11_08985"
     CDS             6281..7213
                     /locus_tag="PLQ11_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate/arogenate dehydrogenase family
                     protein"
                     /protein_id="HRK25072.1"
                     /translation="MTVLFNRLALIGCGLIGSSIARAARKRRLVGTIVVADASESVRE
                     RVKALGLADVVTDDVAAAAGQADCVILCLPVGANAAVAAAIGPHLKAGAIVSDVGSVK
                     AAVVRDVAPHLPVGVHFVPAHPVAGTEYSGPDAGFAELFDNRWSILTPPEGQDPSAIA
                     AMTAFWRGLGANVEVMGAQHHDLVLAITSHVPHLIAYNLVGTAYDLETVVESEVITFS
                     AGGFRDATRLAASDPTMWRDVFLNNREAVLEMVARFQEDLAALSRMIRWGDGEGLFDL
                     FTRTRAVRRSIIASGQDTAAPNFGRSDGQAKSAD"
BASE COUNT         1344 a         2277 c         2397 g         1247 t
ORIGIN      
        1 ggaggcggcg cgatcgtgac cgctggcgga tcgacaggag tcgccggggg tgcaggcggt
       61 ggcggagcag caaccggagg gggcggcgcc tccaccctgg gtccggtcga cggggctgag
      121 gcaaccggag gcggagcggc gggcactgac ggcggggcct ttgcaacacc aggctgagtg
      181 ccggcggtcg ggcgtgccgg cggctcgaac agcgaacccg cgaccaggac gccgagaacg
      241 agagccacac ccgcagccgc cagcccggcg atgatcctgg gccgcaggcc ggaggcggat
      301 cgtccgggtt cgacagctgc cggaggggca gtgtccgatt ggggcgcaag gacggcaggc
      361 ggctcaaccg gagagccgca gccggtgcag aagcgggcaa tttcagcgac tggagcaccg
      421 caacgggggc aataacgcgt catccggacc ctccacgtgc catcggcagc ctgagtgcga
      481 gcgagcgccg gacaagcagg ctcagactgt tgccgagcgc cagccccatg ccgaaaaagg
      541 acaggatcat catcgcctgc ggcgtccgcg ctccggcgag cgccacggcc tgcggcacca
      601 gcagcgtcaa cgcccagatg gcgagaaacc acagggtgcc gcgcgccctg acggtttcat
      661 tttcgacaaa cagcaggttg gtcagtgccc aggcgatgcc gaccaatcca ccggccaccg
      721 cgctcaacac catcatcgcg gtggcaaggg acactcctcc aaaccgagcc atcaatccgg
      781 tgcacaggat gctcgaaagg gcggagaaca gcagcgccca ggctgacagg gtccgcgccc
      841 ggcgaaaggt cagcagcatg acgacaattg acagcgcaaa cagcgccatg ccgacaagtc
      901 cgacaaccct gaagagcagc gcggccgttg gatcaatcac caacagggcg acgcccgctg
      961 cggcggccac cagacccatc agtaaccagc gtttcagcac cgcgaccatg gcccgacacc
     1021 gtcccttcgc cgaaccgacc cagactgcga ttctctccaa cgccggaaat ttgaccttaa
     1081 cctaggtcaa cggaacagcg atggagcgtg agtcaggtcg aggtctcagg cgaggccttg
     1141 cgggggcttc acgcagccag acccttgaac agagccaggc cgtcggtgcc gccggtcaga
     1201 ccctcgatga ggttctccgg gtgcggcatc atgcccagca cattgcgcct gtccgaatag
     1261 atcccggcga tgtggttgag cgagccgttg atgttggcgg cctcgttggt gtcgccggtc
     1321 gcgtcgcaat agcggaaggc gacgcggtcc tcgccctcga gccgcttcag cgtctcggcg
     1381 tcggcggtgt aattgccctc gccgtgggcg atgcagacct cgatgacctg atcgcgccgg
     1441 tagagccgcg tgaacggagt atcggtgcgg ccgactttca gatgctgcat cttgcagagg
     1501 aatttcaggc cggcgttgcg ggtgagaatg ccgggcagga ggcccgattc gcacaggatc
     1561 tggaagccgt tgcagatgcc gagcacgagg ccgccgccgg cggcgtgctt gcgcaccgcg
     1621 tccatgatgt gggcgcgccc ggcgatggcg ccgcagcgca gataatcgcc ataggaaaag
     1681 ccgccaggca gcaccacgag atcggtgccc ctgggcagtt cataatcggc atgccaggcg
     1741 tgactgacgc gggcgccgcc cagtttcagg gcgcgggcca cgtcgccctc gcggttggag
     1801 ccggggaagg tgatgacggc agcgtgcatc acaatacctc gaccttgaaa ttctcgatga
     1861 ccgtattggc cagcagtttt tcggcagact gccgcagaat ggcttcggca gcggccttgt
     1921 cggcagcctt cacctcgatg tcgaacacct tgccctgacg caccgaggcg actccctcga
     1981 ccccgagcga tttcagcgcg ccctcgatcg ccttgccctg cggatcgagg acgccgttct
     2041 tgagggtgac ggtgacgcgg gctttcatgg ggctctccgg gcagcgggta ttgtggccgg
     2101 cagttacacc aataccggct gtgattcaaa cacgcttgat gaggaaaacc gcctgctcgc
     2161 ccagaagatt ccacagccac cacggcgcgt tgaagccgat gcgctcgccg gcggcattga
     2221 gggcgacggc tttttccatc gtggccggga tctcctcgac cagtgcgacg aaatcccgga
     2281 tggtgcagaa atggatgttc ggcgtgtcat accaggcgtg gcggaggttg gcggtcaccg
     2341 gcatgcggcc cttgaagaac aggctggagc gcacgcccca atggccgaag ttcgggaacg
     2401 agacgatggc gtgatggccg atgcgcagca tctgctcgat gaccctgcgc ggattgcggg
     2461 ttgcctgcaa cgtctgcgac aggatgacga aatcgaagca atcgtccgga taatcgccaa
     2521 gatcggtgtc ggcatcgccc tggatgaccg catgcccgtg cgagacgcag gtggcgacgc
     2581 cctcgcggct cagttcaatc ccccgcccgt caacctgccg catgcgcacc agcagcgaca
     2641 gcagggcgcc atcgccgcac ccgacatcga gcacgcgagc gccgggggtg accatttcag
     2701 ccaccacgag gtggtcaacg cgggcctggg tggcgctgga cagatctctg agttcggcat
     2761 cggtcatcac acaccccgcg cggcggccgc cgcttcaaga aagccccgca tggtggcgaa
     2821 cagcgccggt tcgtcgagca gaaaggcgtc gtggcccttg tcgctctcga tctcgacgaa
     2881 cgagaccggg gcaccggcgg cattgagcgc atggacgatg gcgcggctgt ccgaggtggg
     2941 gaacagccag tcggaggtaa acgaaatcac gcagaaacgg gttttcgacc cggcaaaggc
     3001 attggccagc cgcccgccgt gatcggcggc catgtcgaaa tagttcatcg cgcgggtcag
     3061 gtagagatag gaatttgcat cgaaacgctc gacgaagctc gagccctgat ggcgcaggta
     3121 gctttccacc tggaaatcgg catcgaagga aaaggtgcgg ctgtcgcgat cctggagagc
     3181 gcggccgaac ttgcgctgca acgcctgttc cgacaggtag gtgatatgcg ccgccatgcg
     3241 ggccacagcc agccccttct ccgggcgcgt gccctcctcg atatagcggc cgccacgcca
     3301 gtcgggatca gccatcaccg cctgccggcc cacttcgtca aaggcgatat tctgcgccga
     3361 atgcttggca ccggtggcaa tcggcatggc cgagaacacc cgttcgggat agcgcgcggc
     3421 ccagtcgagc acctgcattc cgcccatcga accgccgacg acgctcagga gcttgtcgat
     3481 gccgagatgc tcgatcagca tggcctgggc attgaccatg tcgccgatgg tgatgaccgg
     3541 aaaatcaaga ccaaaggggc gcccggtcgc cgggttgatg gacgacggcc ccgtggtgcc
     3601 catgcaggcg cccaatacgt tggcgcagat gacaaaatag cggttggtat ccatcaaccg
     3661 gccggggccg atcatgatct cccaccagcc gggcttgccg gtgaccgggt gaacgttggc
     3721 aacgtgctgg tcgccagtca gggcatgaca gatgaggatg gcgttggagc gggccgcatt
     3781 cagcgtgccg taggtgttgt aggcaacctg caggggcgcc agaaattgcc ccgattcgag
     3841 ctggagcggg cgatccatgt cgaaacggat caccgcgctg tcggggcttt ctgcttgcga
     3901 atgggcggag ccagcagcct gacgggattt gcgcgccatc gaaccttcac tcacaaaacg
     3961 aacaatttct tcaggccatt cagaatgttc gttatggcga acaattgcaa ggactaacag
     4021 acggcaatgt gtcgctgcgg agtgtgagtt tgcccgaagg ctccgacggc ccggcccggt
     4081 gcttgccgcg cggacgaggc tgtggctttg tggggacccg accgctggcc cattgcagga
     4141 cctcttgatg gcgaccccca aggaacccgg attgcacgaa ttgcggcttg aactcgaccg
     4201 catcgatgcg gcgatgcagg cgctgttgat cgagcgcagc gagatcaccg acaaggttga
     4261 gcaggtcaaa cgccgggccg ggacggcggg ctcggccttc cgacccggcc gcgaggccga
     4321 gatcatgcgc atgctggccg agcggcatcg aggcagctat ccgttcgacg gcaccgagaa
     4381 catctggcgc gtcatcatcg ccacatcgac ctacacgcag gtgccctatc aggtgcacgg
     4441 cgatgtttct ggcggggatg ccccgatgcg cgattcggtg cgcttccact tcggcttcac
     4501 cgtgccgttt ctgccgcatc cggatgcgag tacggtgatc gaggcggtgg cggcaaggcc
     4561 ctccgatctc ggcgtatttc cgcgctcgca aagcatggag accggcgcct ggtggcgcaa
     4621 gctgcacggc ggggaccgac cgaagatcat tgcccggctg ccctttgtcg agcgcgaaaa
     4681 tcacccttcc ggcattccgg tgttcgttat tgcccggccg ctcaaggagg cggcagtgcg
     4741 tgatgagatc gtcgccagtg tcacggtcga acgctggtcg cccaggatcg cccaagccct
     4801 cggcccgctg gcggcccgga tcgaggcgac agtcggcatc gacagcggac tgtccctgct
     4861 ggtcacccat cctggactgg ttgcggctga ggcagtgatt gcggcgctgc ggaatgccgg
     4921 gatggaggcg cgctacgaag aggtcggaag ccacgccgct gccttcgcgg ccgatttgcg
     4981 caaagccggt gacagagggc cggcgagcgc ataagagagc ccaagatcac gccgcatttt
     5041 gatggaatca tcaaaatgcg gataacgtga tcggtatcaa taagttagag catgtcttct
     5101 gcgattagcc ggattccacc taatcgcgac atgctctaga gtcagccaga gagttgttgt
     5161 cccatgccca agtcctcagc gccgcgtccg attccacgtg ccggagtgat ggaaatcgat
     5221 gcctatgtgc ccggcaagag cgcggctccg cccggcgtca aactctacaa gctgtcttcg
     5281 aacgaaacgc cgctcggggc gagcgcaaag gctatttcgg cctacaagga cattgccggc
     5341 aagctcgaat tctatcccga tggcaattcc accgccctgc gcgaggcgat cgcccgccgc
     5401 tatgggctcg atgcatcgcg catcgtctgc ggggcgggtt cggacgaact gctcaatctg
     5461 atcacccatg ccttcatagg tccgggtgac gagggcattt tcaccgagca cggcttcctg
     5521 gtctatcgga tcgcgattac cgcggccggt ggcagaccgg tggtggtcaa ggaacggcaa
     5581 ttcactgcgg atgtcgatgc gattcttgcc gcggtgacag cgcggacgaa agtcgtcttt
     5641 ctcgccaacc cgaacaaccc gaccggcacc tatctgccgt tttccgaggt caagcgcctg
     5701 catgagggct tgcgcgggga cattgttctg gtgctcgatg ccgcctatgc cgaatatgtc
     5761 cggcgcaatg actacgcctc cggcctggaa ctggtcagcg aaagcgacaa tgtggtgatg
     5821 acgcggacct tctccaagat ccacgggctt gccgggctca gaatcggttg ggcctatatg
     5881 ccggcgggca ttgccgatgc gctcaatcgc atccgcggac cgttcaacct gtcggcgccg
     5941 gcgatcgctg ccggagctgc tgccatcgac gattccgccc atatggaggc ggccgccgcg
     6001 cataacgaga cctggaatgc ctggcttacc aaggaacttg ccgggctcgg gctgtcggtc
     6061 actccgtcgg tcggcaattt cctgctgatc cattttccca ggactgccgg ccagaccgcg
     6121 aggcaggcag accagtttct cagcgagcgc ggcttgatcc tgcgtgctgt cggggcttac
     6181 ggcctgccgg atgcgctgcg gatgaccatc ggttcggaag aggcaaaccg caaggtcgtt
     6241 gccgcgctcc gggattttct gggaaaggca tgagcgggtc atgacggtgc tgttcaaccg
     6301 gctggcgctg attggttgcg gcctgatcgg atcgtccatc gcccgtgccg cccgcaagcg
     6361 gcggcttgtt ggcacaatcg tcgtggccga tgcgtccgaa agcgtgcgtg aacgggtgaa
     6421 ggccctcggg cttgccgatg ttgtcaccga tgatgtcgcc gctgcggcag ggcaagccga
     6481 ttgcgtcatc ctgtgcctgc cggtcggcgc caatgcggcc gtggcggcgg cgatcgggcc
     6541 gcatctcaag gctggcgcca tcgtatccga tgtcggatcg gtgaaggctg ccgtggtgcg
     6601 cgatgttgcg ccgcatttgc ccgtgggggt gcatttcgtc ccggcccatc cggtggccgg
     6661 caccgaatat tccggccccg atgccggctt tgctgaactc ttcgacaacc gctggtcaat
     6721 cctgaccccg cccgaggggc aggatccgtc ggccattgcg gcgatgacag ccttctggcg
     6781 ggggctcggc gccaatgtcg aggtgatggg cgcccagcac catgatcttg tgctggcgat
     6841 caccagccat gtgccgcatc tgatcgccta taatctcgtc ggcacggcct atgatctcga
     6901 aacggtggtc gaatcggaag tgattacctt ctcggccggc ggctttcgcg atgcgacgcg
     6961 cctggcggcc tccgacccca cgatgtggcg cgacgtgttc ctcaacaacc gcgaggccgt
     7021 tctggagatg gtcgcgcgct tccaggagga tctggcagcc ttgtcgcgga tgatccgctg
     7081 gggtgacggc gaagggctgt tcgacctgtt cactcgcacc cgcgcggtac gccgctcgat
     7141 cattgcatcc ggccaggata cggcagcgcc gaacttcggc cgaagcgacg gtcaggccaa
     7201 atccgctgac tgataccagt ggccctcaca aatgacctgc cggtcatttc ccgggccgtc
     7261 cctcg
//
LOCUS       DAOMXG010000124         7205 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_5414_length_7205_cov_8.946573, whole genome shotgun sequence.
ACCESSION   DAOMXG010000124 DAOMXG010000000
VERSION     DAOMXG010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 7205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7205
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5414_length_7205_cov_8.946573"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1227
                     /locus_tag="PLQ11_08990"
     CDS             <1..1227
                     /locus_tag="PLQ11_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012329729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRK25073.1"
                     /translation="DCRRFWAKFRAGEVDGEEIAAVEGRLATTAGTCAVMGTASTMAC
                     IAETLGLALPGSAAIPAVHSDRLVAAEKSGVVAAGRIGRPIRPSEIVTEKSVENALRV
                     LMAVSGSTNAVVHLAAIAGRCGITISRERFNQISDETPVLVDLKPVGDGYMEDFHAAG
                     GVGAVLGEIRHLLHLDTINVLGETLGERLSRPLEWVDRKIIRAFDNPVSPVGGLIALE
                     GNLAPDGAIFKRAAATPSLFETEGRAVVFENLEDLAKRIDDPALDVEPDDILVLKNAG
                     PVAAGMPEAGYLPIPKKIAQKGVKDMVRISDARMSGTAFGTIVLHIAPESAVGGPLAA
                     VQNGDRIRLSVRDKRIDLKLSDAEIARRLKTLPRRSEPQRGYRALYARTVQQAPEGCD
                     FDFLSQAGATISTTRG"
     gene            1248..2600
                     /locus_tag="PLQ11_08995"
     CDS             1248..2600
                     /locus_tag="PLQ11_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRK25074.1"
                     /translation="MNLFAKLNQRAAAGRPIRIGMIGAGKFGSMFLAQMLKIPGVHLV
                     GVVDLNPAAAKSNMDYVGWKAERYAARSLDEAAGSGLTHVGDNAEALIDHPATEIIIE
                     CTGNPMAAVEHCLKAFRAGKNVINVTVEADAFCGPGLSAAAAKAGVIYSMAYGDQPAL
                     TCDLVDWAKTCGFTVVAAGRGHKWLPEYRRSTPETVWDHWGLTREQAERGRLNPKMFN
                     AFLDGSKPAIESAAIANATNLVVPRNGLAFPPGSAEDIPQLMRPKAIGGCLDHEGMVE
                     VASCLTADGKPIPYDIRKGVWVCFRGDTDYIRNCFEEYKVITDDTGRYMALYKKWHLI
                     GLELGVSVASVGLRGEATGAAVCFNGDVAAIAKRDLKPGEMLDGEGGYTVSGGLRQAM
                     DSVRGGYLPLGLAHKVKLIRPVRDGEPVTYADVEMDTSSAAYRLRKDMEQTVLSGTGG
                     "
     gene            2636..3532
                     /locus_tag="PLQ11_09000"
     CDS             2636..3532
                     /locus_tag="PLQ11_09000"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRK25075.1"
                     /translation="MRIDLTSLDLFVTVAEERNIARAAARRNIAASAVSKRIHDLELV
                     FGTPLLVRQSRGVVLTPAGEALARHAGNLVGLVSRIAIDLGSFASGMKGEVRLSANPS
                     AITQFLPEMLCAFMARYPEIEIRLEEATTAVSLRRLEDGLADLAIVGWGLDHPAMTYA
                     PFRTDNLALAVPVGHPLTHNPGPVFFGDTVDYDQVGLEYGSSIQARLEEAAANVGRVL
                     RVKLKVGSFDAVRRMVETGIGIAVLPASAIEAYTASMKICAVPLADGWAKRDLRIVTR
                     DAAALPVTASLLHAWLSGMHDV"
     gene            complement(3761..4771)
                     /locus_tag="PLQ11_09005"
     CDS             complement(3761..4771)
                     /locus_tag="PLQ11_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HRK25076.1"
                     /translation="MLHSPAKADPRTVQAGAAANVVRRIGFCLVPDFSFIAFASAIEP
                     LRLANRHCNTQHYAWHLLSDDGLPVTASNGVSLAVDGAVAEAGPLHMLLVAAGIDVEQ
                     QDHTALIRELRRRAVLGAKIGALCTGAHILARGGLLDGHRATIHWENRIGLQSAFPQL
                     SISEELFEIDRNRLTCAGGTAALDMMLSLITSDLGIDVASAVTDQLIHHRIRDKSEVQ
                     RTTLQTRHGVAQPRMIAIVQRMQTSIDAPLSCSRLAREAGISVRQLERMFRRAFAQTP
                     ARYYLNMRLDHARRLLRQTSMSVIDVSLATGFESASHFSKSYGSHFGVSPSSDRTANA
                     PA"
     gene            4887..>7205
                     /locus_tag="PLQ11_09010"
     CDS             4887..>7205
                     /locus_tag="PLQ11_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008123535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRK25077.1"
                     /translation="MKSNARVVVIGGGVVGVSTLYHLARKGWSDVVLVERKELTSGST
                     WHAAGLLPLFNLSYSVGQLHKYSVNLYQTLEAETGQNVGFRQVSNIRLARTPDRWDEF
                     MAYKGVAETIGVRVNVLAPEDVKRLWPLAVTDGVIGAIQHPDDGYIQPADLTQALARG
                     ARARGAEIYRFTTVVGIERKAGRWLVRTDKGDIACDHVVAATGNFARKTGAMVGLDIP
                     VIPVEHQYIVTEPHPAIVERHRQGLPEMGVLRESDSSWYMREENGGLLLGPYEVGAPN
                     CYIDGPSPHSEYELFQEDLDRLGPHIETAIARVPAFGEVGIKKVYNGAIAYTPDGSPI
                     VGPAWDLPNFWINEGHSFGVTAAGGAGWQLAEWIVDGEPTVDMMGVDPRRFGPYAGRG
                     YLRAKTEEAYANVFSVHYPDEERVAARPLKRAPCYDRMAALGAVFGSVYGWERPGWFA
                     REGYELSAADKAYPDVVLNHNHPPRPADGKPPREKWSFRRSNYFDPVGRECLHVRDAV
                     GLQDMTPFAKYEVSGPGALAWLEGLFANTMPKQGRVALCHVLSGNGGVRAEMTVFREA
                     EERFYLVGPGALERHDWDILVKARPRDGTVDLQKVTAQKGVLVLAGPRSREVLQGLTD
                     TDLSNRAFPWLSGQQMSVGAAPVTALRVNYVGELGWELHHPIEMQTIIFDLLIEAGRG
                     AGIRPYGIKAMIALSIEKSYRLIGRELSIEYAALESGLDRFVKLGKPFRGRDALLAWK
                     AKGFSNRFVTMEIHGVTDCDARGSEPVLHKGRL"
BASE COUNT         1244 a         2266 c         2416 g         1279 t
ORIGIN      
        1 gattgccggc ggttctgggc gaagttccgc gccggtgagg tcgatggcga ggaaatcgcc
       61 gcggtcgagg ggcggttggc gaccacggcc ggcacttgcg ctgtcatggg cacggcatcg
      121 accatggcct gcatcgctga aacgctcggc cttgccctgc cgggcagtgc cgccattccg
      181 gcggtgcatt ccgaccgtct ggtcgcggcg gaaaaatcgg gcgtggtcgc ggcggggcgg
      241 atcggcaggc caatccggcc gtccgagatt gtcaccgaaa aatcggtcga gaatgcgctt
      301 cgcgtgctga tggcggtctc ggggtcgacc aacgcggtgg tgcatcttgc ggcgatcgcc
      361 gggcgctgtg gtatcactat ttcccgcgag cgcttcaacc agatctccga cgagacaccg
      421 gtgctggtcg atctcaagcc ggtcggggat ggttacatgg aggatttcca tgccgccggc
      481 ggcgtcggcg cggtgctggg cgagatcagg catctgttgc atctcgatac gatcaacgtg
      541 ctcggcgaaa cgctgggcga gcgcctgtcc cgcccgctcg aatgggtgga tcgcaagatc
      601 atccgggcgt ttgacaatcc ggtctcgccg gtcggcgggc tgatcgcgct cgaaggcaat
      661 cttgctccgg acggcgccat cttcaagcgt gccgccgcca ctccgtcgct gtttgagacc
      721 gagggcaggg cggtggtgtt cgagaacctt gaagacctcg ccaagcgcat tgatgatccg
      781 gcgcttgatg tcgagccgga tgacattctc gttttgaaaa atgccggtcc ggtcgcggcc
      841 ggcatgcccg aagcgggcta tctgccaatc ccgaagaaga tcgcgcagaa gggcgtcaag
      901 gacatggtgc gcatttccga tgcccgcatg tcgggcaccg ctttcggcac catcgtgctg
      961 catatcgccc ccgaaagcgc ggtgggcggg ccgctggcgg cggtgcagaa cggtgaccgc
     1021 atccgcctgt cggtcaggga caagcgcatc gacctcaagc tctctgacgc cgagattgcc
     1081 cggcggctga aaacgctgcc gaggcgcagc gagccgcagc gcggctatcg tgcgctttac
     1141 gcccgcaccg tgcagcaggc gcccgaaggc tgcgatttcg attttctcag ccaggccggc
     1201 gccaccatct ccaccacgcg cggctgattg cccggaagga tcgccccatg aacctgttcg
     1261 ccaagctcaa ccagcgcgcc gccgccggca ggccgatccg catcggcatg atcggtgccg
     1321 gcaagttcgg ctcgatgttc ctcgcccaga tgctgaaaat ccccggcgtg catctggtcg
     1381 gcgtcgtcga tctcaatccc gccgcggcga aatccaacat ggattatgtc ggctggaagg
     1441 ccgagcgcta cgccgcccgc agcctcgatg aggcggccgg aagcggcctc acccatgtcg
     1501 gcgacaatgc cgaggcgctg atcgaccatc ctgcgaccga gatcatcatc gaatgcaccg
     1561 gcaatccgat ggcggcggtc gagcattgcc tcaaggcgtt cagggcaggc aagaacgtca
     1621 tcaacgtgac ggtggaggcc gatgccttct gcggtcccgg cctgtcggcg gcggcggcga
     1681 aggcgggcgt catctattcg atggcctatg gcgaccagcc ggcgctcacc tgcgatctgg
     1741 tcgattgggc aaagacctgc ggttttacgg tggtggccgc cggtcgcggt cacaagtggc
     1801 tgcctgaata ccgccgctcg acacccgaga ccgtatggga tcactggggg ctgacacgcg
     1861 aacaggccga gcgcgggcgg cttaacccga agatgttcaa tgccttcctc gatggttcca
     1921 aaccggcgat cgaatccgcc gccattgcca atgccactaa tcttgtcgtg ccgagaaacg
     1981 gcctcgcctt cccgccgggt tctgccgagg acattccgca gctgatgcgc ccgaaggcga
     2041 tcggcggctg cctcgatcac gagggcatgg tggaggtcgc ctcctgcctg acggcggacg
     2101 gcaagccaat cccctatgac atccgcaagg gcgtctgggt gtgcttccgc ggcgatacgg
     2161 actacatccg caactgcttc gaggaataca aggtcatcac cgatgacacc ggccgctaca
     2221 tggcgcttta caagaaatgg cacctgatcg gcctcgaact gggcgtttcc gtcgcctcgg
     2281 tcggtctgcg cggcgaggcg accggcgccg ccgtctgctt caacggcgat gtcgcggcga
     2341 ttgccaagcg cgacctgaag cccggcgaaa tgctcgacgg cgagggcggt tacaccgtct
     2401 ctggcgggtt gcggcaggcg atggactcgg tcaggggcgg ctatcttccg ctcggccttg
     2461 cacacaaggt caagctcatc cgtccggtca gggatggtga gcccgtcacc tatgccgatg
     2521 tcgagatgga tacctcaagt gcggcctaca ggctgcgcaa ggatatggaa cagacggtgc
     2581 tgtccgggac cggcggctga cgcgccgcaa cagcggcacg caggatgtga ggaccatgcg
     2641 catcgacctg accagtctcg atctgtttgt cacggttgcc gaggagcgca acatcgcccg
     2701 cgctgccgca cggcgcaaca ttgcggcatc cgcagtttcc aagcgcatcc acgatctgga
     2761 actggtcttt ggcacgcccc tgctggtccg tcagagccgg ggggttgttc tgacccctgc
     2821 cggagaagcg ctggcccggc acgccggcaa tcttgtcggc cttgtcagcc ggatcgccat
     2881 cgatttgggc tcattcgcct ctggcatgaa aggggaggtg cggctctcgg ccaatccatc
     2941 ggcgatcacg cagtttcttc cggagatgct gtgtgcgttc atggcacgct acccggagat
     3001 cgaaatccgc ctggaggagg caaccacggc ggtttccctg cgccggcttg aggatggcct
     3061 tgcggatctg gcgatcgtcg gctggggcct agaccatccc gccatgacct atgcaccgtt
     3121 ccgcaccgac aacctcgccc tggcggtgcc ggtcgggcat ccactgaccc acaatcctgg
     3181 gccggtcttt ttcggcgata cggtcgacta tgatcaggtc gggctcgaat acggctcttc
     3241 cattcaggca cgcctggaag aggccgctgc gaatgtcggc agggtgctta gggtcaaact
     3301 caaggtcggc agttttgacg ccgtgcgccg catggtcgaa accgggatcg gcatcgccgt
     3361 tcttcccgca tcggcgattg aagcctacac ggccagcatg aagatctgcg ccgtgccgtt
     3421 ggccgatggc tgggcgaaac gggacctgcg catcgtcacc cgcgatgccg ccgctctgcc
     3481 cgtaacagcg tcgcttttgc acgcatggct ttcagggatg catgacgttt gagatggccg
     3541 ctagagcatg tcgcgatcaa gtggatgccg gtcaatcgca gaagacatgc tctaagttat
     3601 tgataccggt cacgttatcc gcattctgat gattccatca aatgcggcgt gatctagcag
     3661 aaaatacgcg gattgctcat gccggaaggg caggggctgc ggccagttcc gggaagccga
     3721 cttttgatct gacagtgcct cgcggccgcg ggacgggcgg tcaggccggc gcgttggcgg
     3781 tgcgatcgct gctcggcgaa acgccgaaat ggctgccgta gctcttggaa aaatgcgatg
     3841 cggattcaaa gccggtcgcg agcgaaacgt cgatcaccga catgctggtc tgccgcagca
     3901 ggcggcgggc atgatcgagg cgcatgttga gataatagcg ggccggggtc tgcgcgaagg
     3961 cgcggcggaa catccgctcc agctggcgga cggagatacc ggcctcgcgc gccagacggc
     4021 tgcacgacag cggcgcgtcg atgctggtct gcatgcgctg gacgatcgcg atcatgcgcg
     4081 gctgtgccac gccgtgccgc gtctgcagcg tcgtccgctg cacctcgctc ttgtcgcgga
     4141 tgcgatggtg gatgagctgg tcggtcacgg ccgaggcgac gtcgatcccc agatcgctgg
     4201 tgatcagcga cagcatcatg tcgagcgcgg ccgtgccgcc ggcgcaggtc agccggttcc
     4261 tgtcgatctc gaacagttcc tccgagatcg aaagctgggg aaaggccgac tgcagtccga
     4321 tgcggttttc ccagtggatg gtcgcgcgat ggccgtcaag caggccgccc ctggcaagga
     4381 tatgggcgcc ggtgcacagc gcgccgatct tggcgccgag caccgcgcgc cgcctgagtt
     4441 cgcggatcag cgcggtgtga tcctgctgtt ccacatcgat ccccgcagcg acgagcagca
     4501 tgtggagagg gccggcttcg gccaccgcgc catcgaccgc cagcgaaacg ccgttcgagg
     4561 ccgtgaccgg caggccatcg tccgacagca ggtgccaggc atagtgctgg gtattgcaat
     4621 ggcggttggc cagccgcagc ggttcgatgg ccgaggcaaa ggcgatgaac gaaaaatccg
     4681 gcaccagaca gaagccgatg cgccgcacga cattcgctgc ggcgccagcc tggacggttc
     4741 ggggatcggc tttcgcaggg gaatgcagca tggcgagctt gggcagcgac tgaggtcgcg
     4801 aatatgccag agccggtcgc aattcgcaaa gccttcatcg ctccccgcga cggctaatcg
     4861 atgcgaagct tcgggaaagt gcagcgatga aaagcaatgc cagggtggtt gtcatcggcg
     4921 gcggtgtggt cggcgtctcg acgctgtatc atctggcgcg caagggctgg tccgacgtcg
     4981 ttctggtcga gcgcaaggag ctgacctccg gctccacctg gcatgccgcc ggcctgctgc
     5041 cgctgttcaa tctgtcctat tccgtcggcc agctgcacaa atactcggtc aatctctacc
     5101 agacgctgga agccgagacc ggccagaacg tcggcttccg gcaggtgtcc aatatccgtc
     5161 tcgcccgcac cccggatcgc tgggacgagt tcatggccta caagggggtg gcggagacca
     5221 tcggcgtcag ggtgaatgtg cttgcccccg aggacgtcaa gcgcctgtgg ccgcttgcgg
     5281 tcaccgacgg ggtgatcggc gcgatccagc accccgatga cggctacatc cagccggccg
     5341 acctgaccca ggcgctggcc aggggcgccc gcgcgcgcgg cgccgaaatc tatcgcttca
     5401 ccaccgttgt cggcatcgag cgcaaggccg gtcgctggct ggtccggacg gacaagggcg
     5461 acatcgcctg cgatcatgtg gtcgcagcca ccggcaattt cgcgcgcaag accggcgcca
     5521 tggtcgggct cgacatcccc gtcattccgg tcgagcatca gtatatcgtc accgaaccgc
     5581 atccggcgat tgtcgagcgc cacagacagg gactgcccga aatgggggtt ctgcgcgaaa
     5641 gtgactcgtc ctggtacatg cgcgaggaga acggtggtct gctgctgggc ccctacgagg
     5701 tcggcgcgcc caattgctac atcgacggac catcgccgca ctcggaatac gaactgttcc
     5761 aggaggatct cgatcggctc ggcccgcata tcgagaccgc catcgcgcgg gtgccggcct
     5821 tcggcgaggt aggcatcaag aaggtctaca acggcgccat cgcctatacg ccggatggat
     5881 cgccgatcgt cggcccggcc tgggatctgc cgaacttctg gatcaacgag ggccattcgt
     5941 tcggcgtgac ggcggccggc ggcgccggtt ggcagctcgc cgagtggatc gtcgacggcg
     6001 agccgacggt ggacatgatg ggggtcgatc cgcgccggtt cggtccctac gccggtcgcg
     6061 gctatctgcg cgccaagacc gaggaagcct atgccaacgt cttttcggtg cattatccgg
     6121 acgaggagcg ggtggcggcc cgcccgctca agcgcgcgcc ctgttacgat cgcatggcgg
     6181 cgctcggcgc ggtgttcggc tcggtctacg gctgggagcg ccccggctgg tttgcccggg
     6241 aaggttacga actgtcggca gcggataagg cctatccgga cgtcgtgctg aaccacaacc
     6301 atccgccgcg tccggccgat ggcaagccgc cgcgcgaaaa atggtcattc cgccgctcga
     6361 actatttcga ccctgtcggc cgcgaatgcc tgcatgtgcg cgatgccgtg ggccttcagg
     6421 acatgacgcc gtttgccaaa tacgaggtgt cgggccccgg cgcgctcgcc tggctcgaag
     6481 gcctgtttgc caacacgatg ccgaagcagg gccgcgtggc gctctgccac gtcctgtccg
     6541 gcaacggtgg cgtgcgcgcc gagatgaccg tctttcgcga ggccgaggag cggttctacc
     6601 tggtcggtcc gggcgcgctc gagcgccacg actgggacat tctggtcaag gcgcggccgc
     6661 gcgacggcac ggtcgatctg caaaaagtca cggcgcagaa gggcgtgctg gtgctcgccg
     6721 gtccgcgctc gcgcgaagtg ctccaggggc tgaccgatac cgacctgtcg aacagggcct
     6781 tcccctggct gtcgggccag cagatgtcgg tgggggccgc gccggtcacg gcgctgcgtg
     6841 tcaattatgt cggcgaactg ggctgggagc tgcaccatcc gatcgagatg cagaccatca
     6901 tctttgactt gctcatcgaa gcggggcggg gcgccggcat caggccttac ggcatcaagg
     6961 cgatgatcgc gctgtcgatc gagaagagct atcggctgat cggccgcgaa ttgtcgatcg
     7021 aatacgcggc gctcgaatca gggctcgacc ggttcgtcaa gctgggcaag ccctttcgcg
     7081 gccgggatgc gctgctggcc tggaaggcaa aaggcttttc caatcgcttc gtgaccatgg
     7141 aaatccacgg cgtcaccgat tgcgacgcgc gcgggtccga gcccgttctg cacaagggcc
     7201 ggctc
//
LOCUS       DAOMXG010000125         7187 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_5433_length_7187_cov_10.712703, whole genome shotgun sequence.
ACCESSION   DAOMXG010000125 DAOMXG010000000
VERSION     DAOMXG010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 7187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7187
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5433_length_7187_cov_10.712703"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="PLQ11_09015"
     CDS             complement(<1..290)
                     /locus_tag="PLQ11_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRK25078.1"
                     /translation="MFRATVLTLYPDMFPGPLGISLAGEGLRAGLWSLEARNIRDHGH
                     GRHRAVDDTPAGGGPGMVLRADVLAAAIDAAVAAGDPRPRLLMSPRGRPLTQA"
     gene            complement(303..872)
                     /gene="rimM"
                     /locus_tag="PLQ11_09020"
     CDS             complement(303..872)
                     /gene="rimM"
                     /locus_tag="PLQ11_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_026190522.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HRK25079.1"
                     /translation="MRRRRPLTSSPSIPASSGAGTDGLVVMGVFGAAVGLRGEIRVKS
                     YTEDPASIVGYSPLLTGDGRSLTLTLVREQNDMIVVRVEGVTSRQAAEALTNISLHVP
                     RARLGATEADEFFHADLIGLAAVTPSGEPLGTVVAVHDFGAGDMIEVASPRGRTQVFP
                     FTKAVVPVVDLAAGRIVIDPPAEIDGDAP"
     gene            complement(944..1297)
                     /gene="rpsP"
                     /locus_tag="PLQ11_09025"
     CDS             complement(944..1297)
                     /gene="rpsP"
                     /locus_tag="PLQ11_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007693780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HRK25080.1"
                     /translation="MSVKIRLSRGGTKKRPYYYIVVANSTAPRDGRFIEEIGSYNPLL
                     PKDSAERVKLDVDKAKAWLAKGAQPTDRVLRFLDGAGLAKRTPTNNPDKAKPGKKMAE
                     RAEKKAAKAAAPASE"
     gene            complement(1427..2983)
                     /gene="ffh"
                     /locus_tag="PLQ11_09030"
     CDS             complement(1427..2983)
                     /gene="ffh"
                     /locus_tag="PLQ11_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003601551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HRK25081.1"
                     /translation="MFEGLSGRLSGILDRLKGRGALSEADVGEAMREVRRALLEADVA
                     LDVVRSFTDKVRSRAVGAEVIKSVTPGQMVVKIVHDTLVETLGSDADPIDLNAAPPVP
                     VLMVGLQGSGKTTTTAKIARRLTDRMKKRVLMASLDTRRPAAMEQLAQLGRQVGIDTL
                     PIVAGQTAVQIARRAVEAARLGGYDVVMLDTAGRTTVDEALMAEVVEVKAATNPHEVL
                     LVADALTGQDAVNTAKAFESRVGVTGIVLTRVDGDGRGGAALSMRAVTGKPIKLIGTG
                     EKTDALEDFHPSRIANRILGMGDIVSLVEKAAETIDKDEALKIAEKMRKGRFDLADMR
                     SQLLQMEKLGGMGGLMGMLPGIAKFKDQIAQANIDQTVFKRQVAIIDSMTPWERRNPD
                     QLKHSRKKRIAAGSGTSAEDINKLLKMHRGMAEMMKMMGNAPKRGPMAGLANMFGLGG
                     GMPTPSPEQIEALQKQMGGKLPPMPADLTKGIPSLPPGLPGLGKPGLPGLGGKLPGLG
                     GFNPFGGKKK"
     gene            complement(3195..3782)
                     /locus_tag="PLQ11_09035"
     CDS             complement(3195..3782)
                     /locus_tag="PLQ11_09035"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HRK25082.1"
                     /translation="MSRLSAMIVVALGLACAPLAAVHAQQPPAPVAGEPKPSADASSA
                     TEILLQARPVLTLKGQSSWDDGYEALMKAFSALRAEAKRLGLEAVDKPRAVFSETSDT
                     NFRYTAMITLAREPESGVKPASGMTVDRSPEGKAYMFSHVGAYDDIDSIYEAITAWLD
                     EKGLTARGEFIEEYLNEPQGSDDALLELKIYVFVK"
     gene            complement(3844..5160)
                     /locus_tag="PLQ11_09040"
     CDS             complement(3844..5160)
                     /locus_tag="PLQ11_09040"
                     /inference="COORDINATES: protein motif:HMM:NF024841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HRK25083.1"
                     /translation="MRFSALICVVCAGLASAGLADERQAQITPLALPSLPLGVVTFGS
                     GKAVNLSVSPGAGAFRLPSDPPGRIWTVTDRGPSIDCSEERDVIGPDDKLVCQAERRG
                     KIYLLPGFVPSIYAIDIGAEKQARFIDMVPLKGISGKPLTGIANPYVHATSEEAFAPD
                     GQSLTADPSGIEPGALVRLADGRFWIAEGFAPSLLEVAADGRVLRRIVPESLGSDLSS
                     ADYPVEASLPDVLSRRPRGRGFEGLAITPDESYLFGAMSSALVNPDVETYRASPSVRI
                     IKVDRVTGRVVDQFVYVLDPPSAFAVDSGITSRASRQSDVRIVEIAALDANRLLVLER
                     MRRHVKLYRVDLSDARPLPLVYTQSATQPSLEATPRFAWPQSRIEPLPKTLLYANPPA
                     KAFPTKITGMALLSPNDIVLIGGNDFGIDGSRAQMFRLTFAAPVPN"
     gene            5296..6135
                     /gene="dapF"
                     /locus_tag="PLQ11_09045"
     CDS             5296..6135
                     /gene="dapF"
                     /locus_tag="PLQ11_09045"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HRK25084.1"
                     /translation="MIDFVKMNGLGNDFVVIDARRNPMPLGPKQVTLIADRRFGVGCD
                     QLIVLEPSARAQAFMRIFNPDSSEVHACGNATRCIGDYLMAESGADRVWIETGAGLLA
                     AWRSEGGVTVDMGPARLEAAAIPLARTDVDTRRVPIDVDGLGAGLPDWFGAVSMGNPH
                     AVFFVEDVNAHQLERVGPVLETHPLFPQRANVSLVAVAGSDRLIQRVWERGAGLTLAC
                     GSGACAAAVAAHRLGLCGRQVEVTLPGGTLAIDWRPEGSVWMSGGTARAFTGRIDPAL
                     LEG"
     gene            6144..>7187
                     /gene="mtaB"
                     /locus_tag="PLQ11_09050"
     CDS             6144..>7187
                     /gene="mtaB"
                     /locus_tag="PLQ11_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HRK25085.1"
                     /translation="MTRGARRVEVLGFGCRLNLAESAALRGMAEAAGAENLVVVNSCA
                     VTSEAVRQARQAVRKVRRERPGARIVVTGCAAHTEGTAWAGMAEVDAVVANSDKLKAE
                     TWFGVPGAGRAAVEDRERSALSPLAQPIEGRTRGFVQVQTGCDHSCTFCIIPVGRGRS
                     RSVPLGDVVLAVARLVDQGIGDVVLSGVDLTSYGTDPADAPRLGGLVKAILAGVPGLR
                     RLRLSSLDCSEVDAELMRAIIEQDRLMPHLHLSLQAGDDLILKRMKRRHTYADAVQFC
                     DALRRRRPDIVFGADLIAGFPTETEAMFRRSLDLAADCGLTHLHVFPFSPRPGTPAAR
                     MPQVERGEVRERAA"
BASE COUNT         1244 a         2234 c         2441 g         1268 t
ORIGIN      
        1 gcctgggtga gggggcgccc gcgcgggctc atcaaaaggc gcggccgcgg gtcacccgcg
       61 gcgacagcag catcgatggc ggcggccagc acatcggcgc gcagcaccat gcccgggccg
      121 ccgccggccg gcgtatcatc gacagcccga tgacggccgt ggccgtgatc acggatattg
      181 cgggcttcca gcgaccagag cccggccctt aacccctcgc cggccagcga gattccaagc
      241 gggccgggaa acatgtccgg gtagagggtg agaacagtgg cgcggaacat gacggttcac
      301 gctcagggtg catcgccgtc gatctcggca ggcggatcga tgacgatgcg tccggcagcc
      361 agatcgacaa ccggaacaac agccttggtg aacggaaaga cttgcgtccg gccgcgcggg
      421 gaggcgactt caatcatgtc gcctgcgcca aagtcatgga cggcaacgac cgtgccaagc
      481 ggttcaccgg agggtgtaac ggcggccaat ccgatcagat cagcgtgaaa aaactcgtcc
      541 gcttcggtcg cgcccagccg ggcgcgtggc acatgaaggc tgatgttggt cagcgcttcg
      601 gctgcctgtc ggctggtgac cccctcaacg cgcaccacga tcatgtcgtt ctgttcgcgc
      661 acgagggtca gggtaaggct gcgtccatcg cctgtcagca atggcgaata tccgacgatg
      721 gaggctgggt cctcggtata ggatttgacg cggatctcgc ccctcaggcc gacggcggca
      781 ccaaagacac ccatcaccac aagcccatcc gttccagccc ccgaagaggc tgggattgat
      841 ggtgacgagg tgagcgggcg acggcgacgc atcggttcat cctctgcggg cggcacggtc
      901 tgcgccaccc gcggtccgat ccttcccgtt cagaagggac cgtttattcg ctcgccgggg
      961 cagcagcctt ggccgctttc ttctcggcgc gctcggccat tttcttgccg ggcttggcct
     1021 tgtcagggtt gttggtcggc gtgcgcttgg caaggccggc gccatcgagg aagcgcagca
     1081 cgcgatcggt cggctgggcg cccttggcca gccaggcctt ggctttgtcg acgtcgagct
     1141 tgacgcgctc ggccgaatcc ttcggcagca gcgggttgta gctgccaatc tcctcgatga
     1201 agcgaccatc gcgcggcgcg gtcgaattgg cgacgacgat gtagtaatac ggacgcttct
     1261 tggtgcctcc acgcgagagg cggatcttca cggacatcgg gttactcctg ttggtcctac
     1321 gatattggtc ctgacttcgt caccgggagc ttgtcgaacc atgctgtccg aacagtgcgg
     1381 cagcatcgtt cgaccggccc gggatgacgg gatcgagact tgaaactcac ttcttcttcc
     1441 ccccgaacgg gttgaacccg ccaaggcccg gcagcttgcc gccgaggccg ggaaggccgg
     1501 gcttgcccag ccccggcaag ccgggcggca gggaggggat gcccttggtc agatcggccg
     1561 gcatcggcgg cagtttgccg cccatctgct tctgcagcgc ctcgatctgc tccggagacg
     1621 gtgtcggcat gccgccgcca aggccgaaca tgttggccag cccggccatc gggccgcgct
     1681 tgggggcgtt gcccatcatc ttcatcattt cggccatgcc gcgatgcatc ttcaagagct
     1741 tgttgatgtc ctcggcgctg gtgccggaac cagcggcgat gcgcttcttg cggctgtgct
     1801 tgagctggtc agggttgcgc cgttcccagg gggtcatcga atcgatgatc gccacctgac
     1861 gcttgaacac cgtctggtcg atattggcct gggcaatctg gtccttgaac ttggcgatgc
     1921 cgggcagcat gcccatcagc ccgcccatgc cgccaagttt ttccatctgc aagagctggc
     1981 tgcgcatgtc ggcgagatcg aagcgaccct tgcgcatctt ctcggcgatc ttgagcgcct
     2041 catccttgtc gatggtctcc gccgccttct cgacgagcga aacgatgtcg cccatcccga
     2101 ggatgcggtt ggcgatgcgg gaggggtgga aatcctcgag cgcatcggtc ttttcgccgg
     2161 tgccgatcag cttgatcggc ttgccggtca ccgcgcgcat cgacagcgcc gcgccgccac
     2221 ggccatcgcc atcgacgcgg gtcagcacaa tgccggtcac cccgacgcgg ctttcaaagg
     2281 ccttggcggt gttgaccgcg tcctggccgg tcaatgcgtc ggcaaccagc aacacctcgt
     2341 gcggattggt ggcggccttg acctcgacca cctcggccat cagcgcctca tcgaccgtgg
     2401 tgcggccggc ggtatcgagc atcaccacat catagccgcc gagacgggcc gcctcgaccg
     2461 cacgacgggc aatctgcacg gcggtctggc cggcaacgat cggcagggtg tcgatgccaa
     2521 cctggcggcc aagctgggcg agctgctcca ttgcggcggg gcggcgggtg tcaagcgagg
     2581 ccatcagcac ccgcttcttc atccggtcgg tcagccggcg ggcaatcttg gcggtggtgg
     2641 tggtcttgcc cgaaccctgc aagccgacca tcaggacagg aaccggcggg gcggcattaa
     2701 gatcgatcgg gtcggcgtcg gaaccgagcg tctcgaccag ggtgtcatgg acgatcttga
     2761 cgaccatctg gccgggcgtg accgacttga tcacctcggc gccgacagcg cggctgcgca
     2821 ccttgtcggt aaaggagcgg acaacgtcca gggcgacgtc ggcctcgagc agcgcgcgcc
     2881 gcacctcgcg catcgcctcg ccaacatccg cttcgctgag ggcgccccgg cccttcagcc
     2941 tgtcgaggat gccggacagg cgaccggaca agccttcgaa catgccatca ctccgttcat
     3001 gcgacgaaac cgtcgcggca aaccgtttcg cgcccgaggg cgcaaccgcg ctgtcgggcg
     3061 ttgacctccg gctccgcaga gccggtcggc ggatcaattg accagccaga aggaaaagtg
     3121 gagcgcttcc tagccggagc gtgccgaaaa gtcaaaggtt tccggaaagc ccggccgtta
     3181 ccgattgcgc cgggctattt gacaaacaca tagatcttga gttccagcag cgcatcatcg
     3241 gaaccttgcg gctcattgag atattcctcg atgaactcac ctctggcggt cagccccttc
     3301 tcgtccagcc aggcggtgat cgcctcatag atgctgtcga tgtcatcata ggcgccgaca
     3361 tggctgaaca tataggcctt gccttccggc gagcgatcga cggtcatgcc ggatgccggc
     3421 ttgacacctg actcgggttc gcgcgccagc gtgatcatcg cggtataccg gaaattcgtg
     3481 tcgctggtct cgctgaacac cgcacggggc ttgtccaccg cttcaaggcc gagtcgcttg
     3541 gcctcggcgc gcagggcgga aaaggccttc atcaacgcct cgtagccgtc gtcccaggac
     3601 gattggccct tgagagtgag cacggggcga gcctgaagaa ggatctcggt tgccgacgag
     3661 gcatcggcag atggctttgg ctcgcccgct accggagcgg gcggctgctg ggcatgaacg
     3721 gctgcaagcg gagcgcaggc aagtccaaga gcaacgacga tcatcgctga aaggcgcgac
     3781 atggcaggcc cccctttttg cgcccggcgc agaaagggac cgggccaaga tcacgaccgg
     3841 ttctcagttg gggactggcg cagcgaaggt caagcggaac atctgggcgc ggctgccatc
     3901 aatgccgaag tcattgccgc cgatcagtac gatgtcgtta ggcgacagga gcgccattcc
     3961 ggtaatcttg gtcggaaagg ccttggctgg cggattggca tagagcagcg tcttgggcag
     4021 cggctcgatg cgcgattgcg gccaggcaaa gcgcggcgtg gcctcaagcg agggctgggt
     4081 tgccgattgg gtatagacca gcggcaacgg gcgggcatca gaaaggtcga cgcgatagag
     4141 cttcacatgc cggcgcatgc gctccagcac cagcaggcgg ttggcatcaa gggcggcaat
     4201 ctcgacgatc cgcacatcgc tctggcggct ggcgcgcgac gttatgccac tgtctacggc
     4261 aaaggccgag ggaggatcga ggacatagac aaactggtca acgacccggc cagtgacccg
     4321 gtcaaccttg atgatgcgaa cagacggcga ggcccggtag gtttcaacgt ccggattgac
     4381 cagagcgctc gacatcgctc cgaacaggta gctttcatcg ggggtgatcg ccaagccttc
     4441 gaagccgcgg ccgcgggggc ggcgcgacag cacatccggc aggctggcct cgaccggata
     4501 atctgcactg gaaaggtcgc tgccaagcga ttccgggacg atgcgacgca acacccggcc
     4561 atcggcagca acctcaagca gcgaaggagc aaagccttcg gcgatccaga acctgccatc
     4621 ggcaaggcgc accagcgcac ccggttcgat tccggacgga tcggcagtca ggctttgtcc
     4681 gtccggggcg aatgcctctt cgctggttgc gtggacatag ggattggcga tcccggtcag
     4741 cggcttgccg gagatgccct tgagcgggac catatcaatg aagcgggcct gcttttcggc
     4801 gccgatgtca atggcgtaga tcgacggcac aaagccgggc agcagataga tctttccacg
     4861 gcgctcggcc tgacagacga gcttgtcgtc agggccgatg acgtcacgct cctccgaaca
     4921 atcgatcgat gggccgcgat cggtgactgt ccagatgcgg ccgggtgggt ccgaaggcaa
     4981 gcggaaggcg ccggcccccg gggatacgga gagattgaca gccttgcccg agccaaaggt
     5041 caccacacca aggggcaggc tcggcagagc gaggggcgtg atctgcgcct ggcgttcgtc
     5101 ggcaagtccg gccgaggcca gaccggcaca gactacgcat atcagggctg aaaaacgcat
     5161 gtcgacgttc tgcctcctgc gggcgcctca tggtggcggg gtccggacat tgtccgcccg
     5221 acaaaaccca ttatatggga attgcaagac aattatagga aaactccgcc gccaaggcgc
     5281 cacccgagcc agaaaatgat cgatttcgtt aaaatgaacg ggctgggcaa tgatttcgtg
     5341 gtcattgatg cgcgtcgcaa tcccatgccg ctggggccga agcaggtgac gctgattgcc
     5401 gaccgccggt tcggggtcgg ttgcgaccag ctgatcgtgc tcgagccgtc tgcacgggcc
     5461 caggcgttca tgcgcatttt caaccccgac agttcggagg tgcatgcctg cggcaatgcc
     5521 acacgctgca tcggcgacta tctgatggcg gaaagcgggg cggaccgcgt ctggatcgag
     5581 accggtgcgg ggctgctggc ggcctggcgc agcgagggtg gggtcacggt cgatatgggg
     5641 ccggcccggc tggaggcggc ggcgattcca ctggcgcgca ccgatgtcga tacccggcgc
     5701 gtgccgatcg atgtggatgg gcttggagcc ggcctgccgg attggttcgg cgcggtctcg
     5761 atgggcaatc cgcatgcggt gttctttgtc gaggatgtca atgcgcacca gctggaacgg
     5821 gtggggcccg tcctcgagac ccatccgctg tttccgcagc gggcgaatgt ctcgcttgtg
     5881 gctgtcgccg gcagcgaccg gctgatccag cgcgtatggg aacggggcgc cggcctgacg
     5941 ctcgcctgtg gttcgggcgc ctgtgcggca gccgttgcgg cccatcgcct cggattgtgc
     6001 ggccggcagg tcgaggtgac cctgccgggc ggcactctgg ccatcgactg gcggccggag
     6061 ggatcggtgt ggatgagcgg cggcacggcg cgggcattta ccggccgcat cgatcctgct
     6121 ctgctcgaag gctgacgctg gcaatgacaa ggggcgcgag aagggtcgag gtgctcggct
     6181 tcggttgccg gctcaacctt gccgaatccg ctgcgcttcg gggcatggcg gaggcggccg
     6241 gcgcagagaa ccttgtcgtg gtcaacagct gtgccgtcac ctccgaagca gtgcggcagg
     6301 cgcggcaggc cgtccgcaag gtccggcggg aacggcctgg cgcacggatc gttgtgaccg
     6361 gatgtgcggc ccataccgag ggcacggcct gggccgggat ggccgaggtc gatgccgtcg
     6421 ttgccaacag cgacaagctg aaggccgaga cctggttcgg cgtgcctgga gcgggcaggg
     6481 ctgcggtcga agatcgcgaa cgcagcgccc tgtcgccgct ggcccagccg atcgaggggc
     6541 ggacacgcgg cttcgtgcag gtgcagaccg gctgcgacca ttcctgcacc ttctgcatca
     6601 ttccggtggg tcgcggccga agccggtcgg ttccgctggg cgatgtcgtt ctggcggtgg
     6661 ctcgtctggt tgatcaaggc atcggcgatg tggtcctgtc cggcgtcgat ctgacgtcct
     6721 acgggaccga tcctgcagat gcgccaaggc tgggcgggct ggtaaaggcg atccttgccg
     6781 gcgttccggg tttgcgccgg ctgcgcctgt cctcgctgga ttgcagcgaa gtcgatgcgg
     6841 agctgatgcg ggcaatcatc gagcaggaca ggctgatgcc gcatctgcat ctgtcgctgc
     6901 aggctggtga tgatctcatc ctcaagcgga tgaagcggcg tcacacttat gccgatgcgg
     6961 tgcagttctg cgatgccttg cgccgacgac ggccggatat cgtgttcggt gccgatctca
     7021 ttgctggctt tcctaccgaa acggaagcca tgtttcgccg ctcgctcgat ctggctgcgg
     7081 attgcggcct gacgcatctg catgtctttc cattctcgcc gcggccgggc acgccggcgg
     7141 cacgcatgcc ccaggtggag cgcggggaag tgcgcgaacg cgccgcc
//
LOCUS       DAOMXG010000126         7181 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_5438_length_7181_cov_8.855880, whole genome shotgun sequence.
ACCESSION   DAOMXG010000126 DAOMXG010000000
VERSION     DAOMXG010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 7181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7181
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5438_length_7181_cov_8.855880"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="PLQ11_09055"
     CDS             <1..619
                     /locus_tag="PLQ11_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25086.1"
                     /translation="ASLRAAPPLPAEGVATLGVARHLMVRESIPGLMSNRLVHALLDA
                     KRALSGQNPALAQMGAPDLEADAVVRVHSGSRVLFQGEERSLLALAMEWIYAIPLLLG
                     LVGTLVMGIVRWLRPGKAPALQRFVGESLSLRRRAVQATSLAEINATRHAFDRLMAEF
                     EEQGGTGPITAQSTAALTALDLCERQIASRRADLDRVAALTGRPV"
     gene            complement(652..1422)
                     /locus_tag="PLQ11_09060"
     CDS             complement(652..1422)
                     /locus_tag="PLQ11_09060"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HRK25087.1"
                     /translation="MRKSVTGLMLGVAVAAMTLAAAPSAAFDAKQKGEIEQLIKDYLI
                     RNPEVIQEALIELDRRQRDAEMAARQKMLKDEAPALYSSKFNAVIGNPSGDVTLVEFF
                     DYNCGFCKRAVADLERLMKEDPKLKIVLKDLPILSAASRDAAAVALAVKAIAKPDKFW
                     EFHTKLMARTGQIGKAQALEVALAVGIDKARLEEEMGKLDLVKQAFEESRKLADGLAM
                     TGTPAYVLADEVVVGAVGYAQLKARIDNVRKCGKNQCE"
     gene            complement(1457..2842)
                     /locus_tag="PLQ11_09065"
     CDS             complement(1457..2842)
                     /locus_tag="PLQ11_09065"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HRK25088.1"
                     /translation="MQWKRRFASASRRWLAASLALGLFLPPALAQEGTRRTYIRDAEI
                     EHLLNDYLSPLLRAAGQPQGAVKLALVDDRSFNAFVSSGRRMTLFSGAIVDSVTPNQL
                     IGVLAHETGHISGGHLARLSQELPRAQAIAIIGTLLGAGAVVGASRQGNIGLHGNAPG
                     GLMMSGPELAMRSLLAYQRTEEQAADRAAITFLTATRQSGKGMVETFERMASQQMFLA
                     SRIDKYLLSHPLANDRVAAIAEVARQSPYYGAVDPPGLVLRHNLARAKLVAFTGRGEE
                     INRRYPQSDSSLPARYARAIQAHRFSRGGDAQARIDGLIAAQPRNPYFWELKGQNLLE
                     NGQAGAAVPPLRKAVALAPGQPLLRLLLGHALMATNVPANVDQAIKELRVALQRDPEV
                     LEAYQYLAQAYEKRGRTGDAELITAEGYAYAGAIEEAAFIAKRAQAKFPPNSPEWRKA
                     NDIIVQKKDKG"
     gene            3091..4200
                     /locus_tag="PLQ11_09070"
     CDS             3091..4200
                     /locus_tag="PLQ11_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRK25089.1"
                     /translation="MDVMREAGERLRAGEDVVSLAVGEPGAPPPRAVREAVKAALDGG
                     RVPYTDALGLPQLRERIARHYRDAYGVSIDPARVAVTTGSSGGFILAFLSMFDVGDRI
                     AIPRPGYPAYRNILDALGIEVVELETTEATRFAITAAMVEEAHRKTPLKGVLVMSPAN
                     PTGVMMQPSAVADLAATCDRLGLWFLSDEIYHGLTYAGEVTTAAAHSDQAVVINSFSK
                     YYCMTGWRVGWLILPERLVRPAECLAQSLAISVPYLSQIAGIAAFDARDDLEIIKAGY
                     ARNRVRLAEALPAMGLPLMPMDGAFYAYADVSRYSNDSMDFARRALREAGVAITPGLD
                     FDRSEGHRAVRLSYAGSEADIDKAIVRLKVWLGRV"
     gene            4321..4539
                     /locus_tag="PLQ11_09075"
     CDS             4321..4539
                     /locus_tag="PLQ11_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25090.1"
                     /translation="MGPFPRAILIACLSILRWMLLVTAGLVVILLVVQQLRGDAEAAP
                     GALAIFAVITAGIGWLCGRAAVWFERQG"
     gene            complement(4708..5418)
                     /locus_tag="PLQ11_09080"
     CDS             complement(4708..5418)
                     /locus_tag="PLQ11_09080"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRK25091.1"
                     /translation="MIKHHGTSGIDVSDSGDLSGPGVRGLDRRSFLIRSAAGLSVLGL
                     AGCATPDGLSLAEAEKLYGAVPDKKFPIPAADISQLDPKYYRRTVRYDSSEAPGTIIV
                     DPANYYVYRIEGDGNATRYGANVGRQGFLWSGEAYVGRKAEWPTWTPSKEMIKRQPEV
                     AKYARGMPGGPENPLGARTLYLYQKGVYTLYTIYSTSDPETIGTNLTSGCTGLLTQDM
                     IDLYDRTAIKTKVIVLPA"
     gene            complement(5622..6164)
                     /locus_tag="PLQ11_09085"
     CDS             complement(5622..6164)
                     /locus_tag="PLQ11_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRK25092.1"
                     /translation="MTRSEIRLAGSGGQGLILGMHILFRALALQGRRAAQSQSYEPTS
                     RGGFCYSDLIVSDEPGDYPLVTGLDAIAALSQIGLDRSLDLVRPGAVMVVDARLVPEP
                     PATGFAIHVLPISERAVALGSPRVANIVALGALAGLTGLCDPATLEQAVKLETPRKFA
                     DLNLAAVREGFALPRAEVAA"
     gene            complement(6161..7009)
                     /locus_tag="PLQ11_09090"
     CDS             complement(6161..7009)
                     /locus_tag="PLQ11_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HRK25093.1"
                     /translation="MNETVRPPFEIKDYLRLNLMPHFMCPGCGHGIALRALLWAVHEL
                     GIDKNRLAIVSGIGCSGRVGAYIDANTFHTTHGRPLAFATGLKLARPDLTVIVITGDG
                     DCLAIGGNHLIHACRRNLDITCLMLNNEVYGMTGGQVSPTTAANRLTTTTPGGNAEPT
                     FDACKLAEAAGASFVGREVTLQTPALKNLIREAIAHPGFSFVEVMSDCTEIYGRKNDL
                     GESPEMILNQKSDMRPEAFGNVADQPFRPNRLQTGILARLDRPEYGAVYRARTAAERA
                     KPGRNA"
     gene            complement(7002..>7181)
                     /locus_tag="PLQ11_09095"
     CDS             complement(7002..>7181)
                     /locus_tag="PLQ11_09095"
                     /EC_number="1.2.7.3"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HRK25094.1"
                     /translation="TAAAGARAVLVPELNAGQLAIEVERLIGAEKTHRLNRIDGEPIS
                     PDAIAARLRELATDE"
BASE COUNT         1159 a         2317 c         2391 g         1314 t
ORIGIN      
        1 agcctcgctg cgcgccgcgc cgccgctgcc ggccgaaggg gtggcgacgc ttggcgtggc
       61 ccggcatctg atggtgcgcg agagcatacc tggcctcatg tccaaccggc tggtgcatgc
      121 cttgctcgac gccaagcggg cgctgtccgg ccagaacccg gcccttgcgc agatgggggc
      181 gcccgatctg gaagccgatg cggtggtgcg ggtgcatagc ggcagccgcg ttctgttcca
      241 gggcgaggag cgctcgctgc tcgcccttgc gatggaatgg atctatgcca ttccgctgct
      301 tcttggtctg gtcggaacgc tggtcatggg cattgtccgc tggctcaggc cgggcaaggc
      361 gccagcgctg caacggttcg tcggcgaaag cctgtcgctc aggcgccgcg ccgttcaggc
      421 gacctcgctg gccgagatca atgccacgcg ccatgccttc gaccggctga tggccgaatt
      481 cgaggagcag ggcggcacag ggccgatcac ggcacagtcg acggcggcgc tcaccgcact
      541 cgacctgtgc gaacgccaga tcgccagccg gcgagccgat ctcgatcgcg tcgcggcgct
      601 gactggccgg ccggtgtgag gtcagctttc tgaagggtgc cttggtgccc ctcattcaca
      661 ctggttcttg ccgcatttgc gcacgttgtc gatgcgggcc ttgagctggg catagccgac
      721 ggcgccgacc accacctcgt cggccagcac ataggccggc gtgccggtca tcgccagccc
      781 gtcggccagc ttgcgcgatt cctcgaacgc ctgcttgacc agatccagct tgcccatttc
      841 ctcttcgagc ctggccttgt cgatcccgac tgccagcgcc acttccagtg cctgagcctt
      901 gccgatctgg cctgtcctgg ccatcagctt ggtgtggaat tcccagaact tgtccggctt
      961 ggcgatcgcc ttgaccgcca gcgccacagc cgccgcatcg cgcgaggcgg ccgagaggat
     1021 cggcaggtcc ttgagcacga tcttgagctt ggggtcttcc ttcatcagcc gttcgagatc
     1081 ggcgaccgcg cgcttgcaga agccgcaatt gtaatcaaag aactcgacca gcgtgacatc
     1141 gccgctggga ttgccgatca ccgcgttgaa cttcgacgaa tagagcgccg gcgcctcgtc
     1201 cttcagcatc ttctggcggg cggccatctc ggcgtcacgc tggcggcggt cgagctcgat
     1261 cagcgcctcc tggatgactt cgggattgcg gatcagataa tccttgatga gctgctcgat
     1321 ctcgcccttc tgcttggcat cgaaggctgc cgacggggca gcggcaagcg tcatagccgc
     1381 cacagcgacg ccgagcatca ggccggtcac actcttgcgc atcaaatctc tccttggcgg
     1441 gggctggcgc tgaggctcag cccttgtcct tcttctgaac gatgatgtcg ttggccttac
     1501 gccactctgg tgaatttggc ggaaacttcg cctgcgcccg cttggcgatg aacgccgctt
     1561 cctcgatggc gccggcatag gcatagccct cggcggtgat cagttcggca tcgccggtgc
     1621 gaccgcgctt ctcataggcc tgggcgagat actgataggc ttccagcacc tcgggatctc
     1681 gttgcagagc gacccgaagc tccttgatcg cctgatcaac attggccgga acattggtgg
     1741 ccatcagcgc atgcccgaga agaaggcgga gcagtggctg accgggagcc agcgccaccg
     1801 ccttgcgcaa cggcggaacg gcggcgcccg cctggccgtt ctccagcaga ttctgcccct
     1861 tcagctccca gaaatagggg ttgcgcggct gggcggcgat caaaccgtcg atccgcgcct
     1921 gcgcatcgcc gccgcgcgaa aagcggtgcg cctgaatggc ccgcgcatag cgggcaggca
     1981 acgaggaatc cgattgcgga tagcggcggt tgatctcttc gccgcgcccc gtgaaggcca
     2041 ccagcttggc gcgggcgaga ttgtggcgca gcaccagtcc cggagggtcg accgcgccgt
     2101 aataggggct ctggcgggcg acctcggcaa tcgcagcaac gcggtcattg gccagcggat
     2161 gactgagcag gtatttgtcg atgcgcgagg cgaggaacat ctgctggctc gccatgcgct
     2221 cgaatgtttc caccatgccc ttgcccgact gtctggtcgc cgtcaggaag gtgatggccg
     2281 cgcggtcggc cgcctgctcc tcggtgcgct ggtaggccag cagcgagcgc atggccagtt
     2341 ccggcccgct catcatcagc ccgccggggg cgttgccgtg caggccgata ttgccctgcc
     2401 gcgaggcgcc gaccacggcg ccggcgccca gcagcgtgcc gatgatggcg atcgcctgcg
     2461 ccctcggcag ttcctggctg agccgcgcca gatgtccgcc ggagatatgg ccggtctcgt
     2521 gggcgagcac gccgatgagc tggttgggcg tgaccgagtc gacgatcgcg ccgctgaaca
     2581 gcgtcatccg ccggccggaa ctgacgaagg cgttgaacga acggtcatcg accagcgcca
     2641 gcttgaccgc accctgcggc tggcccgcag cgcgcagcag cggcgaaagg taatcgttga
     2701 gcagatgctc gatctccgcg tcgcggatat aggtgcggcg cgtaccctcc tgcgccaggg
     2761 ccggaggcaa gaacaggcca agcgccagac tggccgcaag ccagcggcgc gacgcggaag
     2821 cgaatcggcg tttccattgc atcggcatgt caaattagac ctagaatcga agtcaaagcg
     2881 ggtcaaacat cctctggtgg cctcgccggc ttgtgatcgg tctgccggca tcggccacga
     2941 gcggcgccga ccttgcgcgc tggcgccgtc cgggtatgtg tgctgttcca catcatcaag
     3001 gccatcgtga ggcaatcgcc gccgggccgg gagatccgtc gatgccgccc gccgattgcc
     3061 cggcgcgccc aagtcgcccc gttcattgcc atggatgtca tgcgcgaggc cggggagcgg
     3121 ctgcgggccg gcgaggatgt cgtttcgctg gccgtgggcg agccgggagc cccaccgccg
     3181 cgggcagtgc gcgaggcggt gaaagccgcg ctggacgggg gcagggtgcc ctataccgat
     3241 gcactcggcc tgccgcagtt gcgcgagcgg atcgcccgcc attaccgcga cgcctatggc
     3301 gtgagcattg atcctgcccg ggtcgccgtc accacggggt cctccggcgg gttcatcctt
     3361 gccttcctgt cgatgttcga cgtcggcgac cgcatcgcca ttccacgccc cggctatccg
     3421 gcctaccgca acattctcga tgcgctcggc atcgaggtgg tggaactgga aacgaccgag
     3481 gcgacgcgct ttgccatcac ggccgccatg gttgaggagg cccatcgcaa gacaccgctc
     3541 aagggcgttc tggtgatgag cccggccaac ccgaccggag tgatgatgca accgtcggcg
     3601 gtggccgatc tggcagcaac ctgcgaccgt ctcggcctgt ggttcctgtc cgacgagatc
     3661 tatcacgggc tgacctatgc gggcgaggtc accaccgcag ccgcccattc cgatcaggcc
     3721 gtcgtgatca attccttctc gaaatactat tgcatgaccg gatggcgggt cggctggctg
     3781 atcctgcctg aacgtctggt gcgcccggcg gaatgcctcg cccagtccct ggcgatttca
     3841 gtgccctatc tgagccagat cgccggcatt gccgcgttcg acgcacggga cgatctggag
     3901 atcatcaagg ccggctatgc gcggaaccgg gtccgccttg ccgaggcctt gccggccatg
     3961 ggcctgccgc tgatgccgat ggacggcgcg ttctatgcct atgccgacgt gtcgcgctat
     4021 tccaacgatt ccatggattt cgcccgccgg gccttgcgcg aggcaggcgt cgccatcacc
     4081 cccggtctcg attttgatcg cagcgagggc caccgcgccg tccgcctcag ctatgcggga
     4141 agcgaggcgg atatcgacaa ggcaatcgtc cggctgaaag tttggctggg gagggtgtga
     4201 acgaaggttc tgcgcgcctt cccggatcgc agcgcggccc tggaaaacac aaccgattca
     4261 atcctgccgg aaaatgcgct actgtcagcc ttggaggcac gtccactcca cggagttccc
     4321 atgggtccgt ttccgcgcgc tatcctgatt gcctgcctgt ccatcctgcg ctggatgctg
     4381 cttgtcaccg ccggccttgt ggtgatcctt ctcgtcgtcc agcaattgcg cggcgatgcc
     4441 gaggcagctc cgggtgcgct ggcgatcttc gcggtcatca cggcggggat aggctggctg
     4501 tgcggccggg cggccgtatg gttcgagcgg cagggataga gcgctttcag cgaatttcaa
     4561 ttcacagaac ctgctctatc tttttgtttt tcgcggtttc ttgacgcgaa ccggagacca
     4621 cttcgcttga aaacgctttg tgccagtggt taggaacgaa tgcgacgccg agcagggccg
     4681 aaggcccggt tgtgagggcc gctggtatca ggccggcagg acgattacct tggtcttgat
     4741 tgccgtgcgg tcataaagat cgatcatgtc ttgcgtcagc aggccggtgc agccgctggt
     4801 cagattggtt ccgatcgtct cgggatcgga cgtgctgtag atcgtgtaaa gcgtatagac
     4861 acccttctgg tagagataga gcgtgcgggc gccgagcggg ttttccgggc ctcccggcat
     4921 gccgcgggcg tatttggcca cctccggctg gcgcttgatc atctccttcg acggggtcca
     4981 ggtcggccat tccgccttgc gtccgacata ggcttcgccg ctccacaaaa agccctgacg
     5041 gccgacattg gcgccataac gggtggcgtt gccgtcgcct tcgatgcggt agacatagta
     5101 attggccgga tcgacgatga tcgtgccggg cgcctcgctg gagtcgtaac gcaccgtccg
     5161 ccggtagtat ttcgggtcga gctggctgat gtcggccgca ggaatcggga atttcttgtc
     5221 tggaaccgcg ccgtaaagct tctccgcttc ggccaggctc aacccgtccg gggtggcgca
     5281 cccggccagc ccaagaacgc tgaggcctgc ggccgaacgg atcaggaagg aacgccggtc
     5341 gaggcctctg acacccggtc cggacaggtc gccgctgtcg ctcacatcga tcccggacgt
     5401 tccgtggtgc ttgatcatgc tctcggacct tacttcactc gggggaagcg ggcgccaaca
     5461 tgtcccactc tacgccacaa ccggtcgaga ttgccgccag cgccgctgct tggcaagttc
     5521 gggcttcagg cgctggccta tcggcttgca acagggtcaa caaagtccaa ctgcggaggc
     5581 gccgcgccgg tcgaacaggc atggcaatcc aggcgggcct ttcaggcggc tacctcggcg
     5641 cggggcaggg cgaagccttc gcgcaccgcc gcgaggttga gatcggcgaa tttcctcggc
     5701 gtttccagct tcaccgcctg ttcgagcgtt gccgggtcgc aaaggccggt gaggccggcg
     5761 agagcgccga gcgcaacgat attggcaaca cgcgggctgc cgagtgccac agcgcgctcg
     5821 ctgatcggca ggacatggat ggcaaagccg gttgccggag gctccggcac cagccgcgca
     5881 tccaccacca tcaccgcgcc cggccggacc aggtccagcg agcggtcaag cccgatctgc
     5941 gacagcgcgg cgatggcatc gagcccggtg accagcggat aatcgccggg ctcatcggaa
     6001 acgatcaggt ccgaatagca gaagccgccg cgcgaggtcg gttcgtagct ctgtgactgc
     6061 gccgcccgcc gcccctgcaa cgccagtgcg cggaacagga tatgcatgcc gaggatcagg
     6121 ccctgaccgc cgctgcctgc gaggcggatt tcactgcgcg tcatgcgttt cgtcctggtt
     6181 tggcccgctc ggcggcggtg cgtgcccgat agacggcgcc gtattccggg cgatcgagcc
     6241 gggcgagaat gccggtctgc aaacggttgg ggcggaacgg ctgatcagcc acattgccga
     6301 aggcttcggg ccgcatgtcg cttttctggt tgaggatcat ttcaggggat tccccgaggt
     6361 cgttcttgcg gccgtagatc tcggtgcaat ccgacatgac ctcgacgaag gaaaagccgg
     6421 gatgggcaat cgcctcgcgg atcaggttct tcagcgccgg ggtctgcaac gtcacctcgc
     6481 ggccgacgaa gctcgcccct gccgcctcgg ccagcttgca ggcatcgaag gtcggttcgg
     6541 cattgccgcc cggcgtcgtc gtggtcagcc ggttggcggc ggtggtgggg gaaacctgcc
     6601 caccggtcat gccatagacc tcgttgttga gcatcaggca ggtgatgtcg aggttgcgcc
     6661 ggcaggcatg gatcaggtga ttgccgccga tcgccagaca gtcgccgtcg ccggtgatga
     6721 cgatcaccgt cagatcgggg cgggcgagtt tcagccccgt ggcgaaggcc agcggccggc
     6781 cgtgggtggt atgaaaggtg ttggcgtcga tataggcgcc cacccggccg gaacagccga
     6841 tgcccgagac gatggccagc cggttcttgt cgatgcccag ttcatgcact gcccacagca
     6901 gggcgcgcag cgcgatgcca tggccgcagc cggggcacat gaaatgcggc atcaggttga
     6961 gccgcagata atccttgatc tcgaacggcg gccggacggt ctcattcatc ggtggcaagc
     7021 tcccgcaggc gcgcggcgat ggcatcgggc gaaatcggct cgccgtcgat gcggttgagc
     7081 cgatgggtct tttcggcgcc gatcagacgc tcgacctcga tcgccagttg cccggcgttg
     7141 agttccggca ccagcacggc gcgggccccg gcggcggcag t
//
LOCUS       DAOMXG010000127         7171 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_5449_length_7171_cov_10.324340, whole genome shotgun sequence.
ACCESSION   DAOMXG010000127 DAOMXG010000000
VERSION     DAOMXG010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 7171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7171
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5449_length_7171_cov_10.324340"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..586
                     /locus_tag="PLQ11_09100"
     CDS             8..586
                     /locus_tag="PLQ11_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012652802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HRK25095.1"
                     /translation="MRVIRRAEQSEMPWKNGGGTTREIAASPAGAALDAFDWRISVAR
                     VASDGPFSRFAGIDRSLTVIEGNGMVLDFGDGRSACLTVADPPLAFAGDVPCSARLID
                     GAIGDLNVMTRRGSWRADVVANASLPLLGDPRTDTLLVYLIEGTARAGASDLCAGDAL
                     LLEPGDQAQSITGAPAARVAFVRLIAVNRPSP"
     gene            complement(618..1469)
                     /locus_tag="PLQ11_09105"
     CDS             complement(618..1469)
                     /locus_tag="PLQ11_09105"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007565106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HRK25096.1"
                     /translation="MSAERIAFVNGAFVPLAEARISVMDRGFLFGDGIYEVTAVLDGR
                     LIDNEGHLARLDRSLAAIAIPNPYPAAEWTRLQAELAARNGLTEGLIYLEVTRGVWER
                     DFVSPDDLAPTVVMFTQAKAIRTQAAADSGIRVITAPDQRWERRDIKSVSLLAQVLAK
                     RAARAAGVAEAWMVEDGVVTEGASSTAFIVTADRAIVTRPLSHKVLAGITRASILAIA
                     AERGYRLVERPFTLAEAFAAKEAFITSASTLCHAVIAIDGHIIGEGRPGPVAIELRQR
                     YLAAALA"
     gene            complement(1466..2572)
                     /gene="pyrC"
                     /locus_tag="PLQ11_09110"
     CDS             complement(1466..2572)
                     /gene="pyrC"
                     /locus_tag="PLQ11_09110"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HRK25097.1"
                     /translation="MNPSPPGLDPLFAPSGTDGAAPQTLSIRRPDDWHVHLRDGAMLT
                     AVLPFTARQFRRGIIMPNLVPPVTTVAMASAYRDRILAARPADADFEPLMTCYLTDAT
                     DPDEIARGFAEKVWIAAKLYPAGATTNAHFGVTSIRGLAPVLERMEKIGMPVLVHGEA
                     TDPAVDIFDREAVFMETELLPMLKRHPGLKVVVEHVTTEETTAIVAAHAPRVAGTITP
                     HHLILNRTSIFQGGLRPHLYCLPVAKRERHRLALRKAATSGQPCFFIGTDTAPHPRTA
                     KEASCCSAGVFAGATALQTYVQVFDEEGALDRFEAFASVNGARFHGLPLNEGTIDLIR
                     RPDRLAEAVTVGDSEVVVFRGGEVLPWSLGTVAA"
     gene            2755..3462
                     /locus_tag="PLQ11_09115"
     CDS             2755..3462
                     /locus_tag="PLQ11_09115"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007007494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HRK25098.1"
                     /translation="MNNLSLPVDRAIIAEEAAKMLLEIKAVHFYSDKPFIFTSGWASP
                     VYTDCRKIISFPRLRAALMDFAAAVLVREAGYEQIDVVAGGETAGIPFAAWIADRMGL
                     PMQYIRKKPKGFGRNAQIEGEVIPGQRALLVEDLATDGRSKINFVNALRTAGLTVEHC
                     FVIFFYDIFPGSRQAMADMGVKLHALTTWWDVLKVAKASGHFEPKVLDEVEKFLHEPA
                     KWSAAHGGIADFGSKGE"
     gene            3479..4135
                     /locus_tag="PLQ11_09120"
     CDS             3479..4135
                     /locus_tag="PLQ11_09120"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HRK25099.1"
                     /translation="MATALLDLDGTLVDPALGILNAYCRALAAMGHPVPDRASLTWVI
                     GPALRVSFPKLLGPDADIEAAIGHYRAYYGEIGLYEATPYPGIHAALTALRSAGHRLV
                     LCTAKPEIYARRVVERFDLLPLLDAVFGPDLAGALDDKADLIRHIFATEGRDPAGVVM
                     IGDRMFDIRAAKANGIPAIGVLWGYGTREELAGARRLIAEPADLPEAFAAIVGGGPVR
                     "
     gene            complement(4128..4490)
                     /locus_tag="PLQ11_09125"
     CDS             complement(4128..4490)
                     /locus_tag="PLQ11_09125"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HRK25100.1"
                     /translation="MSAGAGPDRLGQRFYVITASPTGRAGDRHVATEAHLAWLKRLEV
                     SGILFLAGPFVQEDGRSTGGGMFIVRAGSLAEAEELAAGDPYNAGGFRTYSVAPWRLD
                     QGGFALSVSLAGQTVRFT"
     gene            complement(4487..5617)
                     /gene="ribB"
                     /locus_tag="PLQ11_09130"
     CDS             complement(4487..5617)
                     /gene="ribB"
                     /locus_tag="PLQ11_09130"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HRK25101.1"
                     /translation="MLHTHKGWLSSIDELIEDARAGHMFILSDDEDRENEGDLVIPGD
                     MITPEAINFMARYGRGLICLAMTGEQVERLNLTLMAAQNHAPHKTAFTVSIEAREGVT
                     TGISAADRAHTVKVAIDPAATPRSIVTPGHVFPLIAENGGVLVRAGHTEAGVDIARLA
                     GLNPSAVICEIMNDDGTMARMPDLIRFAQTHGLKIGTIADLISHRRRTEKLVDKIAEC
                     QFDSRFGAGFTLKVYASKIGYAEHIALVMGDVSGPEPTLVRVHQHHFLTDSLGDLGGV
                     QKPGELEATMRMIAARGRGVVLIIREPSPAAVSRAVRERAGDPVTGWSGELRHYGIGA
                     QILQDLGVTEMILVSNKRRTIVGLDGYGISLVGHEPIPPVTG"
     gene            complement(5722..6576)
                     /locus_tag="PLQ11_09135"
     CDS             complement(5722..6576)
                     /locus_tag="PLQ11_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRK25102.1"
                     /translation="MSARPSLLPVRFMLRLFAARRLALVVLVAAAASACQRSAPDFTA
                     SIAGQPGWTEDGLKRQAEALGQAYDRKPGEKALSLRYAQVLRQLGQHGQAVAVLQRAS
                     VANLNDAEVAAAYGKALAEVGRFREASEVLARAHMPDRPDWRILSAQGAAADQMGDHA
                     RAQEFYNNALQVSPGEPSVLANLGLSYALTKRLPEAEKVLRQAASHPRADDRVRANLA
                     LVLSLAGKGEGTAASPPPAAAGAPAAGTKAAKSSRPTATVNPALRPGQTNTWAEIAQA
                     EVARQQKN"
     gene            6684..>7171
                     /locus_tag="PLQ11_09140"
     CDS             6684..>7171
                     /locus_tag="PLQ11_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HRK25103.1"
                     /translation="MLHPSLIARRPAGCRPIHLVTAEGWPALAASLSATARGYAEVNG
                     FQPKPGRTLILPNAEGTIEAVVMGIDPAGTPGSDPFVSGSLAATLPEGDYAFDTVPAG
                     AELATLAFLMACYRFDRYKAAEPCRARLVVPKGVDGARVSRIAAAVAMGRDLINTPAA
                     DLG"
BASE COUNT         1203 a         2326 c         2420 g         1222 t
ORIGIN      
        1 ctgatcgatg cgcgtgatcc gccgcgccga gcagagcgaa atgccgtgga agaacggcgg
       61 cggcacgacg cgcgagatcg cggcctcgcc agccggggcc gcgctcgatg cctttgactg
      121 gcggatcagc gtggcccgtg ttgccagcga cgggccgttt tcccgtttcg ccggcatcga
      181 ccgcagcctc accgtgatcg aggggaacgg gatggttctc gacttcggtg acggccgcag
      241 cgcgtgcctc accgtggccg atccgccgct ggccttcgcc ggtgatgtgc cgtgttcggc
      301 ccggctgatc gacggtgcga tcggcgacct caacgtgatg acccgacgcg gttcatggcg
      361 ggcggatgtt gtggccaatg ccagtttacc gcttctcggc gatccccgaa cggataccct
      421 gttggtttac ctgatcgagg ggacagcccg cgccggggcg tcagaccttt gtgccggcga
      481 tgcgctgctg ctcgaacccg gcgaccaggc acagtcaatc accggtgcgc ctgccgcgcg
      541 tgtcgcgttt gtccgcctga tcgcagtgaa ccggccttca ccctgatccg tccgcgaggt
      601 ccgccggccg gcgccgctca ggcgagcgct gcggcaagat agcgttgccg caattctatg
      661 gccaccgggc cggggcgccc ctcgccgatg atgtgcccat cgatggcgat caccgcatga
      721 cacagggtcg aggccgaggt gatgaaggcc tcctttgccg caaacgcctc ggcgagcgtg
      781 aacgggcgct cgaccagccg gtagccccgc tcggcggcta tggcgagaat cgaggcgcgg
      841 gtgatgccgg cgagcacctt gtgcgacaac ggccgggtga cgatggcgcg gtccgccgtg
      901 acgatgaacg ccgtcgagga cgccccttcc gttaccactc catcctcgac catccaggct
      961 tcggcgacac ctgccgcccg agctgcccgc ttggccagta cctgcgcaag cagggacacc
     1021 gacttgatgt cacggcgttc ccagcgctgg tcaggcgccg tgatgacccg gattccgctg
     1081 tccgctgccg cctgcgtgcg gatcgccttg gcctgcgtga acatcacgac cgtcggcgcc
     1141 agatcatccg gcgagacaaa atcgcgctcc catacgccgc gcgtcacttc gaggtagatc
     1201 aacccctcgg tcaggccatt gcgggcggca agttcggcct gaagccgcgt ccattccgcc
     1261 gccggatagg gattgggaat ggcgatggca gccagcgaac ggtcgagccg ggcgagatgc
     1321 ccctcattgt cgatcaaccg cccgtcaagg actgcggtaa cctcgtagat gccgtcgccg
     1381 aacagaaagc cccggtccat caccgaaatt ctggcctcgg ccagcggcac gaaggcaccg
     1441 ttgacgaagg caatccgctc cgcgctcacg cggctaccgt gccgagcgac cacggcagca
     1501 cctcgccgcc gcggaaaacg acgacctcgc tgtcgccgac ggtcactgcc tcggccagcc
     1561 ggtcgggacg gcggatcagg tcgatggttc cctcattgag cggcaggcca tggaagcgcg
     1621 cgccgttgac cgaggcgaag gcttcgaatc ggtcgagagc gccttcctcg tcgaatacct
     1681 gcacataggt ctgcaacgcg gtggctccgg caaaaacgcc ggcagagcag cacgaggctt
     1741 ccttggcggt gcgcggatgc ggcgccgtgt cggtgccgat gaagaagcag ggctggcccg
     1801 aggtcgccgc cttgcgcagc gccagacgat ggcgctcgcg cttggcaacc ggcagacagt
     1861 agagatgcgg ccgcaagccc ccctggaaga tcgaggtgcg gttaaggatg agatgatgcg
     1921 gggtgatcgt gccggcaacg cgcggggcat gggcagctac gatggcggtc gtctcctcgg
     1981 tggtgacgtg ttcaaccacc actttcagcc cgggatgccg cttcagcatc ggcaggagtt
     2041 cggtttccat gaacaccgcc tcgcggtcga aaatgtcgac ggcgggatca gtcgcctcgc
     2101 cgtggaccag gaccggcatg ccgatttttt ccatccgctc cagaaccggc gcaagccccc
     2161 ggatgctggt cacgccgaaa tgcgcattgg tggtggcccc tgccggatag agcttggcgg
     2221 cgatccaaac cttctcggcg aacccgcggg cgatctcatc gggatcggtc gcatcggtca
     2281 gatagcaggt catcagcggt tcgaaatccg catcggccgg acgggcggcg agaatgcggt
     2341 cccgataggc gctggccatg gcgacggttg tgaccggcgg caccagattg ggcatgatga
     2401 tgccgcgccg gaactgccgc gccgtgaacg gcagcacggc ggtcagcatc gccccatcgc
     2461 gcagatgcac atgccagtcg tccggtcggc ggatcgacag ggtttgtggc gccgcgccgt
     2521 ccgtgccgga gggggcgaac agcggatcga gtccgggagg gctggggttc atcggcagga
     2581 ccttttcttg tgttcatccc tgcaacggcc gtgcggctcc gtcaatccgg tagcgccggg
     2641 cggcaccggt ggaaaaaggc ccgcaggccc gccacgcggg gttgatcgtc gccgcgattg
     2701 ccgccaatag ctcatggaga tgaccggcgc ttcccgcgcg ccgagggagg ccccatgaac
     2761 aatctgtcgc tgcccgtcga ccgggccatc atcgccgagg aagcggccaa gatgctgctc
     2821 gagatcaagg cggtgcactt ttactcggac aagccgttca tcttcacctc gggctgggcc
     2881 agcccggtct ataccgattg ccgcaagatc atctcgtttc cgcggctgcg ggccgcgctg
     2941 atggatttcg ccgctgcggt gctggtgcgc gaagccggct atgagcagat cgacgttgtt
     3001 gccggcggcg agacggcggg cattcccttc gccgcctgga tcgccgaccg catgggcctt
     3061 ccgatgcagt atatccgcaa gaagcccaag gggtttggcc gcaatgcgca gatcgaaggc
     3121 gaggtgatcc ccggccagcg cgcactgctg gtggaggatc ttgccaccga cggccgctcc
     3181 aagatcaact tcgtcaacgc cttgcgcacg gcggggctga cggtcgagca ttgcttcgtc
     3241 atcttcttct acgacatctt ccccggcagc cggcaggcga tggccgatat gggcgtgaag
     3301 ctgcatgcgc tgaccacctg gtgggatgtg ctgaaggtcg ccaaggcttc cgggcatttc
     3361 gaaccgaagg tgctcgacga ggtggagaag ttcctgcatg agccggccaa atggtcggcg
     3421 gcgcatggcg gcattgccga ttttggttcc aagggcgaat aggaagggcg cataggactt
     3481 ggccaccgcc ctccttgacc tcgacggcac gctggtcgat cccgcgctcg gcattctcaa
     3541 cgcctattgc cgggcactgg ccgcaatggg ccatccggtg ccggatcggg ccagcctgac
     3601 ctgggtgata ggcccggcgc tgcgggtgag ctttcccaag ctgctcggtc cggacgccga
     3661 cattgaggcg gcgatcggtc attaccgcgc ctattatggc gagatcggcc tctatgaggc
     3721 gacgccctac cctggcattc acgcggcgct gactgcgctg cgcagcgccg gccatcggtt
     3781 ggtgctgtgc accgccaagc cggagatcta cgcgcggcgc gtcgtcgagc ggtttgatct
     3841 gctgccgctg cttgatgcgg tgttcggtcc tgatctcgcc ggtgcgctcg acgacaaggc
     3901 cgatctcatt cgtcatatct tcgcaaccga gggccgtgat ccggccggtg tggtcatgat
     3961 cggtgaccgg atgttcgata tccgcgcggc caaagccaat ggcattcccg ccatcggcgt
     4021 tctgtggggc tatggaacgc gcgaggaact ggccggcgcc cggcggctga ttgccgaacc
     4081 tgccgacctg ccggaggcgt tcgcggcgat tgttggcggc ggcccggtca ggtaaagcgc
     4141 acggtctgcc cggccagact gaccgacaga gcgaacccgc cctgatcgag ccgccacgga
     4201 gcaacggaat aggtccggaa tccgccggca ttgtagggat cgccggcggc cagttcttca
     4261 gcctcggcca gcgagccggc acgcacgatg aacatcccgc cgccggtgga acggccatcc
     4321 tcttgcacga aggggccggc gagaaacagg atgccagaga cctcaagtcg cttgagccag
     4381 gccagatggg cctcggtggc gacatgccgg tcgccggcgc gccctgtcgg cgatgccgtg
     4441 atcacataga agcgctgacc gagccggtcc ggccctgctc cggcgctcat ccggtcacgg
     4501 gcgggatcgg ctcatgcccg accaggctga tgccgtagcc atcaagcccg acgatggtgc
     4561 gccgcttgtt ggagaccagg atcatctcgg tgacgccgag atcctgcaaa atctgcgcgc
     4621 cgatgccgta atggcgcaat tcaccgctcc agccagtgac gggatcgccg gcgcgttcgc
     4681 gcacggcacg gctgaccgca gccggtgacg gctcgcggat gatcaggacg acgccgcgcc
     4741 cgcgtgcggc aatcatccgc atcgtcgctt cgagttcgcc cggcttctgc acgccgccaa
     4801 gatcgccgag cgaatcggtg aggaaatgat gctggtggac gcgcaccagc gtcggctcag
     4861 gaccggatac gtcgcccatg accagagcga tatgctcggc atagccgatc ttcgaggcgt
     4921 agaccttgag ggtgaagccg gccccaaaac gactgtcgaa ctggcattcg gcaatcttat
     4981 cgacgagctt ttcggtgcgg cggcgatggc tgatgagatc ggcgatggtg ccgatcttga
     5041 ggccatgggt ctgcgcaaag cggatgagat ccggcatgcg cgccatggtg ccatcatcgt
     5101 tcatgatttc gcagatcacc gccgacgggt tgaggccggc gagccgcgcg atgtcgaccc
     5161 cggcctcggt atgtccggcg cggaccagca cgccgccgtt ctcggcgatc aaaggaaata
     5221 catgccccgg cgtgacgatc gaccgcggcg ttgcggctgg atcgatcgcc accttgacag
     5281 tatgggcgcg gtcggcagca gaaatgccgg tggtgacccc ctcgcgcgcc tcgatcgaca
     5341 cggtgaaggc ggtcttgtgc ggcgcgtgat tctgcgctgc catcagggtc aggttgagcc
     5401 gttcgacctg ttcgccggtc atcgccaggc agatcagccc gcggccatat ctggccatga
     5461 agttgatagc ctcgggcgtg atcatgtcgc ccgggatgac gagatcgccc tcgttctcac
     5521 ggtcctcgtc atctgacagg atgaacatgt ggccggcacg cgcgtcctcg atgagttcat
     5581 caatcgagga aagccagccc ttgtgggtat gtagcatcgg gaacgtccgg ctggcggagg
     5641 ttggagaccg ccgcgaggaa gatttggcca agacgtaggg gattgaccgg cgccgttcaa
     5701 gctccggaag gcacaacagc cctagttttt ctgctgccgc gcgacctcgg cctgggcgat
     5761 ttccgcccag gtgttggtct ggccagggcg caaagcagga ttgacggtag cggtcggcct
     5821 agaggatttc gctgccttgg tgccagcggc cggagcgcca gcagcggccg gaggcgggct
     5881 cgccgcggtc ccttcgccct tgccggcaag cgagagcaca agagccagat tggcgcgcac
     5941 ccgatcatcg gcgcgcgggt ggctggcggc ttgccgcagc accttctcgg cctccggcaa
     6001 gcgcttggtc agcgcgtagg acagaccaag attggccagc acactcggct cgcccggaga
     6061 aacctgcagg gcgttgttgt agaattcctg ggcgcgggcg tgatcgccca tctgatcggc
     6121 cgcagcaccc tgtgccgaca ggatgcgcca gtctggccgg tccggcatgt gggcacgcgc
     6181 cagcacttcg ctagcctcgc ggaagcggcc aacctctgcg agcgccttgc cataggcggc
     6241 cgcgacttcg gcatcgttga gattggccac cgaggcgcgt tgcagcaccg caaccgcctg
     6301 gccatgctgg ccgagctggc gcaacacctg tgcatagcgc agcgacaggg ctttttcgcc
     6361 cggctttcga tcataggcct ggccaagcgc ctcggcctga cgcttcagcc cgtcctcggt
     6421 ccagccgggc tggccggcga tcgaagcggt gaaatccggc gccgaacgct gacatgccga
     6481 ggccgctgct gcgaccagaa caaccagagc cagtcgccgg gcagcgaaca ggcgcagcat
     6541 gaagcggacc gggagcaacg acgggcgagc agacatgatg gtttggcctt tgcgcaagtg
     6601 acgcaaagat gagaaatagt tcgttaaccc taagccttgg ttaatgtgcc aaggtccgca
     6661 ccccgcccga tcccggagtt tccatgctgc acccctcgct gatcgcccgc cgtccggccg
     6721 gttgccggcc gatccatctg gtcacggcgg agggatggcc ggcgctggcg gccagccttt
     6781 cggcaacggc gcgcggatat gccgaggtga acggctttca gcccaagccg ggacgcaccc
     6841 tcatcctgcc gaacgccgag ggcacgatcg aggcggtggt catggggatc gatccggcag
     6901 ggacgccggg ctccgacccg ttcgtgtccg gcagtcttgc cgcgaccctg ccggagggcg
     6961 actatgcgtt cgataccgtt cctgccggcg ccgaacttgc aacactcgcc ttcctgatgg
     7021 cgtgctatcg cttcgatcgc tacaaggcgg ccgagccttg cagggcgagg ttggtcgtac
     7081 ccaagggcgt cgatggcgcc cgcgtcagcc ggattgcggc cgcggttgcg atgggccgcg
     7141 acctcatcaa tacgccggcc gccgatcttg g
//
LOCUS       DAOMXG010000128         7001 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_5648_length_7001_cov_8.574143, whole genome shotgun sequence.
ACCESSION   DAOMXG010000128 DAOMXG010000000
VERSION     DAOMXG010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 7001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7001
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5648_length_7001_cov_8.574143"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="PLQ11_09145"
     CDS             <1..269
                     /locus_tag="PLQ11_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide-binding domain containing protein"
                     /protein_id="HRK25104.1"
                     /translation="KLDALFAATARSAVEGGICRLVVAGGETSGAVVSALGLDALAIG
                     PEIDPGVPALATTAGQPLALALKSGNFGAENFFEKALSIMGDAA"
     gene            266..898
                     /locus_tag="PLQ11_09150"
     CDS             266..898
                     /locus_tag="PLQ11_09150"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004115498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HRK25105.1"
                     /translation="MSREADLRETMARLARSLFDRGFTVGSSGNISAAVEDGLLITPT
                     NSCLGFLDPARIAKLDGDGRHVGGDKPSKEVFLHRAFYETRPATGAVVHLHSTHATAL
                     SCLADLDPDDCIPPLTPYVVMRVGTVKLLPYVRPGDPAMGDMIRALNGSHACVLLANH
                     GPVVAAKDLESAVYASEELEETAKLVLTLRGQTVRMLTKDDVAELKRTFG"
     gene            1001..2242
                     /locus_tag="PLQ11_09155"
     CDS             1001..2242
                     /locus_tag="PLQ11_09155"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HRK25106.1"
                     /translation="MTPNKSGHIRLTSHPGTGAPARFPIDWGASEAKGRGPVIATVTS
                     GGDRNAIGAHGGSYALYRALAVSAGVLNPIARPDLTNTHPVVAIGPFPQWSEPGRIVS
                     LDPYGHMVPEVFAGEIAEGVDIRPSIAITRARLTVPELKDRLKVDGTIVREVGDVSAV
                     KIAIDPVWFLPGIADRFGTGETALRRTLFEQTGGMYPELVTRPDLKVFLPPIGNITVY
                     LFGDVARLGQQRTFVSGRLHDECNGSDVFGSDICTCRPYLVHGIEEAVRDAQAGGVGF
                     IVYNRKEGRALGEVTKFLVYNARKRQDGGDQASTYFERTECVAGVQDARFQQLMPDVM
                     HWLGIRRIDRFMSMSNMKHDALVEQGIELVERVPIPADLVPPDASVEMEAKKAAGYFT
                     PGAAPTEEDLKNTVGRRIDEF"
     gene            2274..3488
                     /locus_tag="PLQ11_09160"
     CDS             2274..3488
                     /locus_tag="PLQ11_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="URC4/urg3 family protein"
                     /protein_id="HRK25107.1"
                     /translation="MTPARSLLTPAAVRARAREMLALGTAGRLTAFTVHLDRLPAAAD
                     RVVETIRQNYPDLAVPFHARWRHFCFGGRDLWQERTAELSDAFGQARAAFDLAIVSVL
                     LDAGAGMAWRYRDAALGIEAARSEGLALASLRLFESGALSLRPSEPFRADAARLALIE
                     PADIAAAFQVTEANPLVGVEGRAALLNALGRAAARLPGARPGGLFDGLVNAAARTGEG
                     RNALPAPAILAAVLHHLGPIWPSRITLDGVPLGDTWRHPLIAGDDPATAGLVPFHKLS
                     QWLSYSLIEPLQAAGIAVTDIDGLTGLPEYRNGGLFVDTGVLALRDPAMAGAANTVDA
                     PLVVEWRALTVALLDAIAPLVRERLGKTEAQFPLACVLEGGTWAAGRRLAAERRPDGG
                     SPIRVISDGTVF"
     gene            3501..4130
                     /gene="upp"
                     /locus_tag="PLQ11_09165"
     CDS             3501..4130
                     /gene="upp"
                     /locus_tag="PLQ11_09165"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HRK25108.1"
                     /translation="MKLVNVVDHPLVQHKLSLMRDKDLSTRSFRQLLKEIGMLMAYEV
                     TRELPIHMVEIETPLTRMQAPMIDGKKLVLAPILRAGLGFVEGMLELVPSARVAHIGL
                     YRDPASLAAVEYYFKAPPDVAERLVIVLDPMLATANSAIAAVERLKERGVTNLRFVCL
                     LASRQGIASFHAHHPDVPVWAAAVDPDLNDHGYIVPGLGDAGDRMFGTK"
     gene            4201..4932
                     /locus_tag="PLQ11_09170"
     CDS             4201..4932
                     /locus_tag="PLQ11_09170"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HRK25109.1"
                     /translation="MNETARSLRPVSGTQSRTADLVRMLSDDIRAGRIKPGEKLPTEQ
                     EIVQLTGVSRTVVREAVAALRAEGLVVTRQGVGAFVTAAPGQGLFKIASEDAESIERV
                     LQILELRIGLEVEAAGLAARRADPDSVRMIEEAHEAFRLGAVAGEIVIGADYGFHKAI
                     CEATGNPYFPRILSSLGQILIPRQQVPSSLPTLEARKEYLAGIAGEHGAIFEAIARHD
                     EEGARKAMRHHLTISSDRYRSMKSF"
     gene            5054..5482
                     /locus_tag="PLQ11_09175"
     CDS             5054..5482
                     /locus_tag="PLQ11_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HRK25110.1"
                     /translation="MSRLPTLNSPFMLGFDEIERALDRVAKAANDGYPPYNIERIPRG
                     ESDPERLRITLAVAGFSRDQLEITLEENQLVIRGRQHDDKTRHYLHRGIAARQFQRSF
                     LLADGMEVLGADLRDGLLAIDLARPEPERIIKKIEILGRE"
     gene            5632..5907
                     /locus_tag="PLQ11_09180"
     CDS             5632..5907
                     /locus_tag="PLQ11_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018264521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1150 domain-containing protein"
                     /protein_id="HRK25111.1"
                     /translation="MPDRNASHKVATPLDVTPQELAAIGGGQVAYVKPMSSDEVARLY
                     PGAPQLAPGLKLFALLSADGSPILLTDTEDAAIAGAWEHQLRTVALH"
     gene            complement(5961..6743)
                     /locus_tag="PLQ11_09185"
     CDS             complement(5961..6743)
                     /locus_tag="PLQ11_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRK25112.1"
                     /translation="MTDKLQFLDCSHPAGSVRLAVQARQGKAPAILWLGGFMSDMTST
                     KAAALDAWAAAQGHASVRFDYSGHGTSGGRFEDGTIGQWLDEALALIARFGGPAPVLA
                     GSSMGGWIALLATLRLRATGSPQHPSGLVLIAPAVDFTQALMWAAFPAEVKRQIETTG
                     LWHRASEYSETPYPITRALIEDGRRHLLYGKPFHLGCPVHILQGGLDPDVPQAHALKL
                     VEHLPQDPVTVTLIPDGNHRLSRDEDIATLVRVTAAMAGGAA"
     gene            6832..>7001
                     /locus_tag="PLQ11_09190"
     CDS             6832..>7001
                     /locus_tag="PLQ11_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HRK25113.1"
                     /translation="MQAIVPTLSAEADVLAGKTILQIIPDLDAGGAERTTIDIAEALA
                     RVGARALVASEGG"
BASE COUNT         1126 a         2282 c         2319 g         1274 t
ORIGIN      
        1 acaagctcga tgcgctgttt gccgccaccg ccagatcggc ggtcgagggt ggcatctgcc
       61 ggctggtcgt ggccggcggg gagacttccg gagcggtcgt ctcggcgctt gggcttgatg
      121 cgctggcgat cggcccggag atcgaccccg gcgttccggc gctcgccacc accgccggcc
      181 agccgcttgc gctggcgctc aagtccggca atttcggcgc cgagaacttt tttgaaaagg
      241 cgctttcgat catgggtgat gcggcatgag ccgggaagcg gacctgcgcg agactatggc
      301 gcggctggcc agaagcctgt ttgaccgcgg cttcacggtc ggatcatcgg gcaatatctc
      361 ggctgcggtg gaggatggtc ttctgatcac accgaccaat tcctgtctcg gctttctcga
      421 tccggcccgc attgccaaac tcgatggcga cgggcggcat gtcggcggcg acaagccgtc
      481 gaaggaagtg tttctccacc gcgccttcta tgagacccgg cccgccaccg gcgccgtggt
      541 gcatctgcac tcgacccatg cgacggcgct gtcctgcctt gccgatcttg acccggacga
      601 ttgcatcccg ccgctgacgc cctatgtggt gatgcgcgtc ggcacggtga agctgctgcc
      661 ctatgtccgc cccggcgatc cggcgatggg cgacatgatc cgggcgctga atgggtcgca
      721 cgcctgcgtg ctgctcgcca accacggccc tgtggtggca gcaaaagacc tcgaaagcgc
      781 cgtctatgct tccgaggaac tggaggaaac ggccaagctg gtgctgaccc tgcgcggcca
      841 aaccgtccgt atgctgacga aggacgacgt ggcggagctg aagcggacct tcggctgagc
      901 cgcagttcag gctctgtcaa tcacggacag aattgccgct tgccgaacgg cgcgacgcac
      961 ttcacactcg gtttttgaga ccgccgtcag gggcccgtcg atgacgccga acaagtccgg
     1021 ccatatccgt ctcacctctc atcccggaac cggcgcgccc gcgcgcttcc ccatcgactg
     1081 gggcgcttct gaagcgaaag ggcgcgggcc ggtgatcgcc actgtcacct ccggcggcga
     1141 ccgcaacgcc atcggcgccc atggcggatc ctacgcgctt tatcgcgcgc tggcggtctc
     1201 ggcgggcgtg ctcaatccga ttgcccggcc ggatctgacc aacacccatc cggtcgttgc
     1261 catcggcccg tttccgcaat ggtcggagcc ggggcgcatc gtctcgctcg atccctacgg
     1321 ccatatggtg ccggaggtct ttgccggcga gatcgccgag ggcgtggata tccgcccctc
     1381 catcgccatc acccgggcgc ggctgaccgt gcctgaactg aaagaccggc tgaaggttga
     1441 cggaaccatc gtccgcgagg ttggcgacgt ctcggcggtc aagatcgcca ttgatccggt
     1501 ctggtttctg cctggtatcg ccgaccgttt cggcaccggc gagacggcgc tgcgccgcac
     1561 cctgttcgag cagaccggcg gcatgtatcc cgaactggtc acccgccctg atctcaaggt
     1621 ctttcttccg ccgatcggca acatcaccgt ctatctgttc ggcgatgtgg cgcgtctggg
     1681 gcagcagcgc accttcgttt cgggccgcct gcatgatgaa tgcaacggct cggacgtttt
     1741 cggttcggac atctgcacct gccggcccta tctcgtccac ggtatcgagg aagcggtgcg
     1801 cgatgcgcag gcgggcggtg tcggcttcat cgtctacaac cgcaaggaag gccgcgcgct
     1861 cggcgaagtg accaagttcc tcgtctacaa tgcgcgcaag cggcaggatg gcggcgatca
     1921 ggcatcgacc tatttcgaac gcaccgaatg cgtcgccggc gtgcaggatg cgcgcttcca
     1981 gcaattgatg cccgatgtca tgcactggct cggcatccgc cgcatcgacc gtttcatgtc
     2041 gatgtcgaac atgaagcatg acgcgctggt cgagcagggt atcgagctcg tcgagcgcgt
     2101 gccgattccg gccgatctgg tgccgcccga tgcttcggtt gaaatggagg ccaagaaagc
     2161 ggcaggctat ttcacccccg gcgcggcccc caccgaagag gatctgaaaa acaccgtcgg
     2221 ccgcaggatc gacgaattct gaacagcccg atgaccatgc cggagcgcat ttcttgaccc
     2281 ccgcccgatc cttgctgacg cccgctgcgg tgcgggcacg cgcccgcgaa atgctcgcac
     2341 tcggaactgc gggcaggctg acggcgttca ccgttcacct cgatcgcctg ccggcggcgg
     2401 cggatcgcgt ggtcgagacg atccgacaga attatcctga tcttgccgtg ccgttccatg
     2461 cccgctggcg gcatttctgt tttggcgggc gcgatctctg gcaggagcgg accgctgaac
     2521 tgtccgatgc cttcgggcag gcgcgcgccg ctttcgatct ggcgattgtc agtgtgctgc
     2581 tcgatgccgg agcgggcatg gcctggcgct atcgcgatgc ggcgctgggg atcgaagccg
     2641 cccgctcgga ggggctggcg ctggcctcgc tccggctgtt cgaatcgggg gcgctgtcct
     2701 tgcgtccgtc cgagccgttc cgcgccgatg cggcacgcct tgcactgatc gagccggccg
     2761 acattgccgc cgcctttcag gtgaccgagg ccaatccgct ggtcggtgtc gagggccgtg
     2821 ccgcgctgct gaatgcgctg ggtcgggcgg cggcacgact gcccggtgcc cggccgggcg
     2881 ggctgttcga tgggttggtc aacgccgctg cccggacggg ggaggggcgg aacgctctgc
     2941 ccgccccggc gatccttgcc gcagtgctcc accatctcgg cccgatctgg ccgagccgca
     3001 tcacgctcga cggcgtgccg ctgggcgata cctggcggca tccgctgatt gcgggcgacg
     3061 atccggcgac ggcgggtctg gtgccgttcc acaaactgtc gcagtggctg agttattcac
     3121 tgatcgagcc gttgcaggcg gcgggcatcg ccgtcaccga catcgacggg ctgaccggcc
     3181 tgccagagta ccgcaatggc gggctgttcg tggataccgg cgtcctcgcc ctgcgcgatc
     3241 cggcgatggc gggcgccgcc aacacggtcg acgctccgct ggttgtcgaa tggcgggcgc
     3301 tgacggtagc gctgctcgat gccatcgccc ctctggtgcg cgagcggctg ggcaagacgg
     3361 aggctcagtt cccgctcgcc tgcgtgcttg aaggcggcac ctgggctgcc gggcggcgac
     3421 tggctgccga gcgccggccc gatggcggat cgccgatccg cgtgatatcg gatggcactg
     3481 tgttctgaga ggcagggtcc atgaaacttg tcaatgtggt cgatcacccg ctggtgcagc
     3541 acaagctgag cctgatgcgc gacaaggatc tttcgacccg cagcttccgc cagctgctca
     3601 aggaaatcgg catgctgatg gcctatgagg tcacccgcga actgccgatt cacatggtcg
     3661 agatcgaaac cccgctgacg agaatgcagg cgccgatgat cgacggcaag aagctggtgc
     3721 tggcgccgat cctgcgcgcc ggtctcggct ttgtcgaggg catgctcgaa ctggtgccga
     3781 gcgcccgcgt cgcccatatc gggctctatc gcgatccggc ctcgctggcg gcggtggaat
     3841 attacttcaa ggctccgccc gatgtcgccg agcggctggt gatcgtgctc gatccgatgc
     3901 tggcgaccgc caattccgcc attgcggcgg tcgaacggct gaaggagcgc ggagtgacca
     3961 acctgcgctt tgtctgcctg ctggcctcgc ggcagggcat tgccagtttc catgcgcatc
     4021 atccggatgt tccggtctgg gcggcggcgg tcgatcctga cctcaacgat cacggctaca
     4081 tcgttcctgg ccttggtgat gccggcgatc gcatgttcgg cacgaaatag cagtccgccg
     4141 gagatttgcg tcctgccatc gcttatccta cacaggagcg atggggcggg agagagcctg
     4201 atgaacgaga cagcacggtc cctgcgaccg gtgagtggca cacagagccg cacggccgat
     4261 ctggtgcgca tgttgtcgga cgatattcgc gccggccgca tcaagccggg cgagaagctg
     4321 ccaaccgagc aggagatcgt gcagttgacc ggggtcagtc gcacggttgt ccgcgaggcg
     4381 gtggccgccc tgcgcgccga ggggctggtc gtcacccgcc agggcgtcgg cgccttcgtc
     4441 accgctgcgc cggggcaggg cctgttcaag atcgcctcgg aagatgccga atcgatcgag
     4501 cgtgtgctgc agatcctgga attgcgcatc gggctcgagg tcgaggcggc cgggctggcg
     4561 gcacgccggg ccgatccgga ttcggtccgc atgatcgagg aggcgcatga ggcgttccgg
     4621 ctgggcgctg tggcgggcga aatcgtcatc ggtgccgatt acggcttcca caaggccatc
     4681 tgcgaggcga ccggcaaccc gtattttccg cgcatcctgt catcgcttgg ccagattctc
     4741 atcccgcgcc agcaggtgcc ctcgtcgctg ccgacgcttg aggcgcgcaa ggaatacctt
     4801 gccggcatcg ccggtgaaca tggagcgatc ttcgaggcga ttgcccgcca tgacgaggaa
     4861 ggcgcccgca aggccatgcg tcaccacctg accatctcgt cggatcgcta tcgctcgatg
     4921 aagagtttct gacgcgacaa ttggcgttct cgcctcttgc acggggacgc tgaccatccc
     4981 atattccgcg catggccggc gctcgacagg ggtcggcctt tacgaagtgc caggatggtt
     5041 caagggcttc gagatgtcgc gccttccaac gctgaattcg ccgttcatgc tgggctttga
     5101 cgagatcgag cgggcgctcg atcgggtcgc caaggctgcc aatgacggtt atccacccta
     5161 caatatcgag cgtattccgc gcggcgagag tgatcccgag cggttgcgca tcacattggc
     5221 ggtggcaggg ttctcgcgcg atcagctgga gatcacgctg gaggaaaacc agctggtcat
     5281 ccgcgggcgt cagcacgacg acaagacccg ccactacctt catcgcggca tcgccgcgcg
     5341 ccagttccag cgcagtttcc tgctggccga tggcatggag gttctgggcg ccgacttgcg
     5401 cgacggcttg ctggcgatcg atctcgcccg ccccgagccg gaacggatca ttaagaagat
     5461 cgaaatcctt gggcgcgaat aatcggccct ggatcacgcc ctcgttttga ttgaatcatc
     5521 aagacgagag ataacgtgat ctacgtcaat ggtttagagc atgctttccg cgaaaaaccg
     5581 ttcacacttt ttcgtggcat gctctcgcga cttctgtaag gagcgatgcc catgccggat
     5641 cgcaacgcat ctcataaggt tgccacgcct ctcgacgtca cgccgcagga actggcggcc
     5701 atcgggggcg ggcaggtcgc ctacgtcaag ccgatgtcct ccgatgaggt ggcccgtctc
     5761 tacccgggcg caccgcaact ggcgccgggc ctcaagctgt ttgccctgtt gtcggcagat
     5821 ggctcgccca tccttttgac cgacaccgag gatgccgcca tcgccggcgc ctgggagcac
     5881 cagctccgca cggtggcgct gcactgagac cagcgaccgt cgatcggcct gccggacaag
     5941 ctcgccccgc tacgccatga tcacgccgct ccgccggcca tcgcggcagt gacgcgcacc
     6001 agcgtggcga tgtcctcgtc gcgcgacagc cggtgattgc cgtcggggat cagcgttacc
     6061 gtcaccggat cctgcggcag gtgctcgacc agcttcaagg catgcgcctg cggcacgtca
     6121 gggtccagcc cgccttgcag gatatgcacc gggcagccga ggtggaacgg cttgccataa
     6181 agcaggtgcc ggcgtccgtc ctcgatcagc gcgcgggtga tcggataggg tgtttcggaa
     6241 tactccgatg cccggtgcca caatcctgtg gtctcgatct gccgtttcac ctcagcgggg
     6301 aaagcggccc acatcagcgc ctgggtgaaa tccaccgccg gggcgatcag gacgagaccc
     6361 gaaggatgct gcggcgagcc cgttgccctg agccgcagcg ttgccagcag cgcgatccag
     6421 ccgcccatgg acgaccccgc cagcaccggc gccgggccgc cgaaccgggc gatcagcgcc
     6481 agcgcctcgt cgagccattg cccgatggtc ccgtcctcga accggccgcc gctggtcccg
     6541 tggccggaat agtcaaaccg caccgaggca tggccctgcg ccgcggccca ggcatccagc
     6601 gccgccgcct tggtcgaggt catgtccgac atgaagccgc caagccagag gatcgccggc
     6661 gcctttcctt gccgggcctg tactgccagc cgcaccgatc cggcgggatg gctgcaatcg
     6721 agaaattgaa gcttgtccgt catggctgta ggagggtcct tgcgaatggt gccgatgtgg
     6781 gcgccgattc ggcttgtcgc atgccggagc ggatccggcg agagttttgc aatgcaggcg
     6841 atagtgccga ccctgagcgc cgaggccgat gttctggcgg gcaagaccat ccttcagatc
     6901 attccggatc tggatgcggg tggggctgag cgcacgacca tcgacattgc cgaggcgctg
     6961 gcccgcgtcg gcgcccgggc gctggtggcc agcgagggcg g
//
LOCUS       DAOMXG010000129         6996 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_5653_length_6996_cov_8.494021, whole genome shotgun sequence.
ACCESSION   DAOMXG010000129 DAOMXG010000000
VERSION     DAOMXG010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 6996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6996
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5653_length_6996_cov_8.494021"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="PLQ11_09195"
     CDS             <1..204
                     /locus_tag="PLQ11_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25114.1"
                     /translation="GGWRGGRGWHGPRAFHRPVHWRPRPVHRRVYWGPRIRPVYYAPY
                     PRCFRRGWVWNGYRYVWRRYRVC"
     gene            complement(224..991)
                     /locus_tag="PLQ11_09200"
     CDS             complement(224..991)
                     /locus_tag="PLQ11_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRK25115.1"
                     /translation="MSAEPTALVATTDDLLFAGRLRLRQPARGHRAGSDAVLLAAACP
                     AGARRVVDLGCGAGTVGLRVAQVEDAAEVCLVDRDPALLALTNENAGANGLAARCRTV
                     AGDCLDRRFAASGAGLSGWAEVVLTNPPFYVPGAGTVSPERHKAGAHVLSGDLRDWVK
                     GGLRCLAPRGTIVLIHRADALVSVIEALGGRFGGLKIRPVHPRAEAPAHRMLVLGRRG
                     SRAAPVILPPLVLHGPDGRFTPLAAALHEGTARLAWE"
     gene            1069..2091
                     /locus_tag="PLQ11_09205"
     CDS             1069..2091
                     /locus_tag="PLQ11_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRK25116.1"
                     /translation="MGVVVPIDNKSGKTGRDGIDALVRLVKADMERVNAMILSRTGSD
                     VTMIPEVANHLIESGGKRLRPMLTLATAILSDYSGEGHIKLAAAVEFMHTATLLHDDV
                     VDESEMRRGKKAARMLWGNEASVLVGDFLLGQAFKMMVEVGSLACLDVLSTAAAVIAE
                     GEVMQLGAAKNTATSEDEYLAVIRAKTAALFAAAAEVGPILACLDKPAQVAARSYGMH
                     LGVAFQLVDDALDYGGASATLGKNVGDDFREGKITLPVVLSYRRGSDAERSFWNRTLA
                     RGEIEDGDLERAIGILRSHQALEDTVERARHYGAMAKDALALFPASPMRTALEDVVDF
                     CVARAY"
     gene            complement(2127..3035)
                     /locus_tag="PLQ11_09210"
     CDS             complement(2127..3035)
                     /locus_tag="PLQ11_09210"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HRK25117.1"
                     /translation="MQKGLPWLELAPAKVNLSLHVVGRRDDGYHALESLVAFADLGDS
                     LTLEPDRPLALAVTGPGASSSGPIAENLVMKAVEAMVRAMPGLRLGALSLTKLLPVAA
                     GLGGGSADAAAALRLLGRLNGIAADDSRLFDIARTIGADVPVCLASTTRMMTGAGEAL
                     GPPLALPPLLAVLVNPGRPVATEQVFRALGLAVGTRLDGAGHPVIDCAEDMDSLVAAL
                     APLRNDLQPPAIAIEPSIAEAIEHIRAQEGCRLVRMSGSGATCFGIFGNLSQTFEAVR
                     NLRMKHSRWWVQPAILGGRYPVRSGL"
     gene            complement(3035..4879)
                     /locus_tag="PLQ11_09215"
     CDS             complement(3035..4879)
                     /locus_tag="PLQ11_09215"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRK25118.1"
                     /translation="MPVNLSGRASSLAIVAAFLAGIIAAGPVQARESPLAVRSEALDA
                     TSGSFLAAMSAQGQRDLRAAARYYRDVLQARPDEAIIAERAFIAELGDGNIAEAIRYA
                     ERGVKRGQVNSLARATLGVRDIKQRRFASARQHLARASGGRGRNSDLTVALLTAWTHV
                     GSGDLRRALEIVDQFNAPELTAYRNFFGGLMADVAGDRTEAAKRLTAAYRSEPGTLRV
                     ADAFARLQSRLGNREDAIKVYGEWENRSQGQPFVARQLEDLRAGRALPPLATTVAEGA
                     SEVLYGLGATSGSSREADTSLVFLQLASYLDPDDDLITVSIAEVFEQMRQWERAADAF
                     GRIGAQSPYRIRSLLGRVIAYERMERTDEAIATLTTLVTESPKEYEAVDMLGGLYRTK
                     KKFEESIAVYSRAINALEKPERAHWALFFGRGVAYERNKQWLKGEPDFIKALELLPAK
                     PRMARERYERAQVLNYLGYSWVDQGMNIEQSFDMLKEAVALAPDDGAIVDSLGWAYYR
                     LDRFDDAVRELERAIELKPGDPTINDHLGDAYWQVGRTREAYFKWTHARDLKPEPEDL
                     PRILTKLVHGLDEPPPVLPPELDPGLPDFGKPGRASPPIDRLPSPDDR"
     gene            complement(4995..6683)
                     /locus_tag="PLQ11_09220"
     CDS             complement(4995..6683)
                     /locus_tag="PLQ11_09220"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HRK25119.1"
                     /translation="MESDMERPARERMDYDVVVVGGGPAGLATAIRLKERAAEAGAEV
                     SVVVVEKGSEIGAHILSGAVIDPVALDQLFPDWRNDSSCPVKTPVVEDHFLALGPSGD
                     ITLPNAIMPPLLSNHGNYIASLGNLCRWMAAQAEALGVEIYPGFAASEVLYDAAGAVI
                     GVATGDMGVSREGEMKDSYTRGMELAGKYVVFAEGARGQLSKQVIARFKLDSGRQPQK
                     YGIGIKELWQVAPDRHRKGLVQHSFGWPLDSRTGGGSFLYHFDDNLVSVGFVVHLNYA
                     NPTLSPFDEFQRYKTHPAIRPTFEGGKRLSYGARALTEGGYQSVPKLSMPGAVLVGCG
                     AGFMNVPRIKGSHNAMLSGMLAADHILEALAAGRSGDELTSYDKAWRGSEIGRDLWKV
                     RNVKPLWSRFGTWAGIALGGLDMWMNELVKLSLFGTQSHGKPDHASLKKLSEVKPIVY
                     PKPDGVVSFDKLSSVFLSSTNHEEDQPCHLRLADPAIPVGRNLPDYGEPARLYCPAGV
                     YEIVYADEANRRDPRFVINAQNCVHCKTCDIKDPAQNITWTAPEGGGGPNYPNM"
     gene            6818..>6996
                     /locus_tag="PLQ11_09225"
     CDS             6818..>6996
                     /locus_tag="PLQ11_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRK25120.1"
                     /translation="MDERGTELAALRALLEWYDEAGVDLALDEAPHDRFADSAAERAE
                     AASPRRASPALPAAAA"
BASE COUNT         1259 a         2322 c         2279 g         1136 t
ORIGIN      
        1 ggtggctggc gtggcggacg cggctggcac gggccgcgcg cgttccaccg tccggtccac
       61 tggcggcccc gtccggtcca tcgccgggtc tattggggac cgcgcatccg cccggtctac
      121 tacgcgccct atccgcgctg cttccgccgt ggctgggtct ggaacggcta tcgctacgtc
      181 tggcggcgtt accgcgtctg ctgagttgga cggcgggcgg ccgtcactcc caggcgagcc
      241 gcgctgtgcc ctcatgcagc gcggcggcaa gcggggtgaa gcggccgtcc ggcccgtgca
      301 gaacgagcgg cggcaggata accggcgccg ctcgcgagcc tctccgcccc agcaccagca
      361 tacgatgcgc cggagcctcg gcccggggat gaaccgggcg gattttcaac ccgccgaacc
      421 ggccgcccag cgcctcgatg accgagacca gggcatcagc ccggtggatc agcacgatcg
      481 tgccgcgcgg cgcaagacag cggagcccac ccttcaccca atccctcaga tcgccagaca
      541 acacatgggc tccggccttg tgccgttccg gggagaccgt gccggcgccc ggcacataga
      601 agggcgggtt ggtcagaacc acctcggccc agccggaaag tccggctccg ctcgcagcaa
      661 agcgccggtc aaggcaatcg ccggcaaccg tgcgacagcg cgccgcaaga ccattggcac
      721 ccgcattctc gttcgtcagc gccagcaatg ctggatcccg atccaccaga cagacctcgg
      781 ccgcatcctc aacctgcgcg acacgcaggc cgaccgtgcc ggctccacag ccaagatcga
      841 caacgcgacg ggctccggcc ggacaggcag cggccagcag caccgcatcc gacccggcgc
      901 gatggccccg cgccggctgg cgcagccgca ggcgcccggc aaaaagcagg tcgtctgtgg
      961 ttgcgacaag ggcggtgggc tcggccgaca ttgacggaaa ggttgcattt cgtcgcgtcc
     1021 cggtgcaagc gttggctgac gagaggctga agcatacggg tttttggcgt gggtgtcgtt
     1081 gttccgatcg acaacaagag cggcaagaca ggacgggacg gcattgatgc gctggtccgg
     1141 ctggtcaagg ccgacatgga gcgtgtcaac gcgatgatcc tgtcgcgcac gggatcggat
     1201 gtgacgatga tcccggaagt cgcgaaccac cttatcgaat ccggcggcaa gcggctcagg
     1261 ccaatgctga cgctggcaac cgctatcctg tccgactatt ccggagaggg ccatatcaag
     1321 ctcgccgccg ccgtcgaatt catgcatacc gcaacgctgc tgcatgatga cgtggttgac
     1381 gaaagcgaga tgcgccgcgg caagaaggcc gcccgcatgc tgtggggcaa cgaggcgagc
     1441 gttctggtgg gggatttcct gctcggccag gcgttcaaga tgatggttga ggtcggctcg
     1501 ctcgcctgcc ttgatgtgct gtcaacggcc gccgccgtga tcgccgaggg cgaggtgatg
     1561 cagcttggcg ccgccaagaa caccgccacc agcgaagacg agtatctggc cgtcattcgc
     1621 gccaagacgg cggcgctgtt tgccgctgcg gcggaggtcg gaccgatcct ggcctgtctc
     1681 gacaagccgg cacaggtggc ggcgcgcagc tacggcatgc atctcggtgt ggcgttccag
     1741 ctggttgacg atgctctgga ttatggcggg gccagcgcga cgctcggcaa gaatgtcggc
     1801 gatgatttcc gcgagggcaa gatcaccctg ccggtcgtcc tgtcctaccg gcgcggcagt
     1861 gacgccgagc gcagtttctg gaaccgcacc ctggccaggg gcgaaatcga ggatggcgac
     1921 cttgaacggg cgatcggcat cctgcggtcg catcaggcgc tggaggatac cgtcgagcgc
     1981 gcgcgccatt atggtgccat ggccaaggat gcgctggcgc tgttcccggc ttctccgatg
     2041 cgcactgccc tggaggatgt ggtcgatttc tgcgtggcgc gggcctatta gagcaaaagc
     2101 cgatctgact ggatcgctct tttgctctag aggccggaac gcaccgggta tcgcccaccg
     2161 aggatcgccg gctgaaccca ccagcggctg tgtttcatgc gcaggttgcg gaccgcctcg
     2221 aaggtctggc tgagattgcc gaagattccg aagcaggtgg cgcccgaacc cgacatgcgg
     2281 accagccggc agccttcctg cgcgcgaatg tgctcgatcg cctcggcgat ggagggttcg
     2341 atggcgattg ccggcggctg gaggtcgttg cgcagaggcg cgagcgcagc gaccagactg
     2401 tccatatcct cggcgcaatc aatcaccgga tggccggcgc catccagcct cgtgccaacc
     2461 gcaagtccga gggcgcggaa aacctgttcg gtcgcaaccg gccggccggg attgaccaga
     2521 acggccagaa gcggcggcag cgcgagcggc gggcccagcg cctcgccggc tccggtcatc
     2581 atgcgcgtgg tcgatgccag acagaccggc acatcggcgc cgattgtgcg ggcaatgtcg
     2641 aacagacgcg aatcgtcggc ggcgattccg ttgagccggc ccagcagtcg caaggctgct
     2701 gcggcatcgg cggagccgcc cccgaggccg gcagccaccg gcaacagctt ggtcagggac
     2761 aatgctccca gccgcaggcc cggcatggcg cgaaccatgg cctcgaccgc cttcatcacc
     2821 agattctcgg cgatcgggcc gctgctggat gcgccagggc cggtcaccgc cagcgccagc
     2881 ggccggtccg gctccaacgt cagcgaatcg ccgagatcgg caaaggccac caggctttcc
     2941 agcgcatggt agccatcgtc gcggcgaccg acgacatgca gcgacagatt gaccttggcc
     3001 ggcgccagct caagccacgg cagtcctttc tgcatcagcg atcgtccggc gaggggagcc
     3061 tgtcgatggg cggactggcc cgcccgggct tgccgaaatc cggcaggcca ggatcaagct
     3121 ccggcggcag gacgggcggc ggctcgtcga gaccgtgcac aagcttggtc aggatgcgcg
     3181 gcagatcctc cggctccggc ttgagatcac gcgcatgggt ccatttgaaa taggcctcgc
     3241 gcgtgcgccc gacctgccag taggcatcgc ccagatgatc gttgatggtg ggatcgcccg
     3301 gcttcaattc gatggcgcgc tcaagctccc gcaccgcgtc gtcaaacctg tcgagccggt
     3361 aataggccca gcccagactg tcgacaatgg cgccgtcgtc gggggcgagc gcaaccgcct
     3421 ccttcagcat gtcgaaggac tgttcgatgt tcatgccctg atcgacccag gaatagccca
     3481 gatagttgag cacctgcgcc cgttcataac gctcgcgggc catgcgcggc ttggccggca
     3541 gcaattccag cgccttgatg aaatcgggct cgcccttcag ccattgcttg ttgcgttcat
     3601 aggcgacgcc ccgcccgaag aacagcgccc agtgggcgcg ctcaggcttt tcaagtgcgt
     3661 tgatggccct ggaatagacc gcgatcgact cctcgaattt cttcttggtg cggtagaggc
     3721 cgccgagcat gtcgaccgcc tcgtattcct tcggcgactc ggtgaccagc gtcgtcaggg
     3781 tggcgatggc ctcgtcggtg cgctccatcc gctcataggc gatgacgcga cccagcagcg
     3841 agcggatccg atagggcgac tgggcaccaa tgcggccgaa ggcatcggca gcccgctccc
     3901 attggcgcat ctgttcgaaa acttcggcga tcgaaaccgt tatgaggtcg tcatcagggt
     3961 cgaggtagct ggcaagctgc aggaagacca gcgacgtatc ggcctcgcgg ctcgacccgg
     4021 aggtggcacc aaggccgtag agcacctcgg aagcgccctc ggcaacggtc gtggccagcg
     4081 gcggcagggc gcgaccggcc cgcaggtcct caagctgacg cgcaacgaag ggctgtccct
     4141 ggctgcggtt ctcccactcg ccatagacct tgatcgcgtc ctcgcggttg ccgagccgtg
     4201 attgcaggcg ggcaaaggca tccgccacac gcaaggtgcc cggctccgaa cggtaggccg
     4261 ctgtcagacg cttggctgcc tcggtccggt ctccggcgac atcggccatc agcccgccaa
     4321 agaaattgcg ataggccgtc agctccggcg cgttgaactg atcgacaatc tccagcgcgc
     4381 gcctgagatc cccggagccg acatgggtcc aggccgtcag aagggccacg gtcagatcgc
     4441 tgttgcgccc gcggccaccg ctggccctgg ccagatgctg gcgggccgac gcaaagcggc
     4501 gctgcttgat atcgcggaca cccagcgttg cacgcgccag cgaattgacc tggcctcgtt
     4561 tgacgccccg ctcggcatag cggatcgctt cggcgatgtt gccatcgccc agttcggcaa
     4621 tgaaggcgcg ttcggcgatg atcgcctcgt caggccgcgc ctgcagcacg tcccggtaat
     4681 agcgcgcagc agcgcgcaga tcacgctggc cctgcgcgct catcgcggca agaaaactgc
     4741 cggaggtggc atcaagcgcc tcggagcgca cggcaagcgg gctttcccgc gcctgcaccg
     4801 gcccggcggc gatgatccct gccagaaacg ccgcgacgat ggcaagggac gaggctcgcc
     4861 ccgacaggtt gactggcaaa cccttgctcc catgtcagtt tgccggcaga atcactgccg
     4921 gcgagatcaa atcgttggca caatgacggt tttgccgggc gccggcaagc acagggctgg
     4981 cggcgcccct cgccttacat attgggatag ttcggaccgc cgccgccctc cggcgccgtc
     5041 caggtgatgt tttgtgccgg atccttgatg tcgcaggtct tgcagtgaac acagttctgc
     5101 gcattgatca cgaagcgcgg atcacgcctg ttcgcctcgt cggcgtagac gatttcgtag
     5161 acaccggcag gacaataaag ccgcgccggc tcgccgtaat cgggcaggtt gcggccgacc
     5221 ggaatcgccg ggtcggccag ccggagatgg cagggctggt cttcctcgtg attggtgctc
     5281 gacaggaaca ccgacgacag cttgtcaaat gaaacgacgc catccggctt gggatagacg
     5341 atcggcttga cctcggagag cttcttcagc gaggcatggt caggcttgcc gtgggactgg
     5401 gtgccgaaca gcgagagctt caccagttcg ttcatccaca tgtcgagccc gcccagcgcg
     5461 atccccgccc aggtgccgaa gcgtgaccac agcggcttga cattgcgcac cttccacaga
     5521 tcgcggccga tctcggagcc gcgccaggcc ttgtcatagc tggtcagttc atcccccgaa
     5581 cggcctgcgg ccagcgcctc caggatatga tcggcagcca gcatgcccga cagcatcgca
     5641 ttgtgactgc ccttgatgcg cggcacgttc atgaagccgg cgccgcaacc gaccagcacc
     5701 gcgccgggca tcgaaagctt cggcactgac tggtagccgc cctcggtcag cgcacgcgcg
     5761 ccgtaagaca gacgcttgcc gccctcgaag gtcgggcgga tcgccggatg ggtcttgtag
     5821 cgctggaact cgtcgaacgg cgacagtgtc gggttggcat agttgagatg aacgacaaag
     5881 ccgaccgaga ccagattgtc atcaaaatga tagaggaacg aaccgccgcc ggtcctcgaa
     5941 tcaagcggcc agccgaagga atgctgcacc agccccttgc ggtgcctgtc cggcgcaacc
     6001 tgccagagtt ccttgatgcc aatgccgtat ttctgcggtt ggcggccgga atcaagcttg
     6061 aaccgggcga tcacctgttt ggacaactgg ccgcgcgcac cctcggcaaa caccacatat
     6121 ttgccggcca gttccatgcc gcgggtgtag ctgtccttca tctcgccttc gcgcgacacg
     6181 cccatgtcac cggtggccac gccgatcacc gcgccggcag catcatagag cacttcgctg
     6241 gcggcaaaac cgggatagat ctcaacgccg agcgcttcgg cctgcgcggc catccagcgg
     6301 cagagattgc ccagcgaggc aatgtagttg ccatgattgg acagcagcgg cggcatgatg
     6361 gcattcggca gggtgatgtc gccggaaggc cccagcgcca ggaaatgatc ctcgaccacc
     6421 ggcgtcttga ccgggcagga agagtcattg cgccaatcgg gaaacagctg gtcgagcgcg
     6481 accggatcga tcaccgcccc ggacaggata tgcgcgccga tttcggagcc cttctcgaca
     6541 accaccaccg acacttcggc tccggcttcg gcggcccgct ccttcagccg gatcgcagtg
     6601 gccaggcccg caggcccacc gccaaccaca accacatcat agtccatccg ctcacgcgcc
     6661 ggccgttcca tgtcgctctc catccggacg atagttcata tataaactat ctttgactct
     6721 gacaagcgca cagcttgccg cagctgaccc ttgctagccc ggacaggcgc gctagaaaag
     6781 gctttcacag ccgcagggcg ggcaggccga tacgacaatg gacgaacggg gcacagaatt
     6841 ggccgcgctc agggcgctgc tggaatggta tgatgaagcc ggcgtcgacc tggcgctcga
     6901 cgaggcaccg catgaccgct ttgccgattc cgccgccgaa cgggcggagg cggccagtcc
     6961 gcgccgcgcc tcccctgccc tgcctgctgc ggcggc
//
LOCUS       DAOMXG010000130         6982 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_5668_length_6982_cov_10.030027, whole genome shotgun sequence.
ACCESSION   DAOMXG010000130 DAOMXG010000000
VERSION     DAOMXG010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 6982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6982
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5668_length_6982_cov_10.030027"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(242..2452)
                     /locus_tag="PLQ11_09230"
     CDS             complement(242..2452)
                     /locus_tag="PLQ11_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HRK25121.1"
                     /translation="MKLTHFRFDADAEGLVTLTWDSPGKTMNVINEEWMKELDSVIDT
                     VAGDAKLKGCVIVSGKPDSFSGGADLNFLQSIGAFYQKMLKTDGEDKANARLLTETAR
                     LSQLFRKLETCGKPFVAAIHGVCLGGAFELALSCHHRIASDSDKTKVGLPEIKVGLFP
                     GAGGTQRVARLMQSGDALQLLFRGDQIKGLMARGMGLIHEVVPQDKLVEAAKAWIRGG
                     GSAKAPWDTETYRLPSNKVHTAQGMMIWPPANAIYRRETFDNYPAARAILQVVYEGLQ
                     LPMDLALRVESRYFAKIIRSKEAAMMIRSLFVSKGELEKGARRPAGVAPTSIRKIGVV
                     GAGFMGAGITYVSALAGLEVVLIDRDQEAADKGKAFSQKLLSDQVMKGRATTAHRDAV
                     LGRIHPTPDYAALKDVDLIVEAVFEDRQVKADVIAKVEAVIGKDVIFASNTSTLPITS
                     LAETSSRPDQFVGIHFFSPVDKMLLVEVIKGGRTGDRAIATALDFVRIIKKTPIVVND
                     TRGFFANRCVLNYIREGQLMLMEGVPAPMIETVAKMAGMPVGPLSLMDEVALDLGWKI
                     LQATKKDLGPKAVDPKQEALLEEMVVKRGRLGRKNGKGFYDYPEGKGQKRLWAGLRDL
                     QPRLKDPETIDVQEMKDRLLAIQALEAARTVEENVITDVREADVGSILGFGFAPFTGG
                     AISFIDGMGTKAFVALCDALAKKHGNRFAVNPQLRDMAARGETFYGRQAGKKAA"
     gene            complement(2465..3673)
                     /locus_tag="PLQ11_09235"
     CDS             complement(2465..3673)
                     /locus_tag="PLQ11_09235"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HRK25122.1"
                     /translation="MTDAFIYDHVRTPRGKGKADGSLHEVTALKLAVAPLKALKERNN
                     LDPTLVEDVVLGCVDPVGESSAVIARSAAIAAGYGKTVPGVQINRFCASGLDAVAFAA
                     AQVMAGQKQMAIGGGIESMSRIGMGSSGMLPAADPDVAIPSYFSPQGVAADLISTKYG
                     FTRTQCDQFAVDSHHKAAKAWAEGRFAKSVIPVKDVNGITILDRDETVRPEANMQALG
                     ALKPSFVMMGEQGGFDAVAIQGHPDVEFIEHMHHAGNSSGIVDGASAVLVGSKAAGKK
                     AGLTPRARIVGFASLGSDLALTLTGPTDVTLKLFKNTKMSLKDIDLIEINEAFSSVVL
                     RYLQSLDIDPDIVNVNGGAIAMGHPLGATGGMILGTVIDELERRNKAVGLVTLCVAHG
                     MGSAMIVERV"
     gene            complement(3761..5548)
                     /locus_tag="PLQ11_09240"
     CDS             complement(3761..5548)
                     /locus_tag="PLQ11_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HRK25123.1"
                     /translation="MPIYKAPVEDVAFLLNDVFHIGRYSNLPGFSDLSPEMLDAILGE
                     IAKLCEEQLQPLNMSGDQEGCVRHPDGSVTTPKGFKDAYKAYVDSGWLSLACNPEYGG
                     QGLPYTMAAIVNEFASAANMSFSMYPGLTAGAIAAIDVHGSPELKATYLPKMIAGTWT
                     GTMNLTEPHCGTDLGLMRTKAAPQADGSYKITGTKIFISAGEQDITENIIHLVLARIE
                     GAPEGVKGISLFIVPKFVPDANGNPGARNAVACGSIEEKMGIHANSTCVMNYDGATGF
                     LIGEANKGLNAMFVMMNEARLGVAIQGLAQSEVAYQNAVAYARERIQGRSLTGVKNPE
                     KAADPIIVHPDVRRTLLTIRSFNEGARAFMLWGALKSDVAHRGGDEKARADSDELLGL
                     LTPVLKAFLTDRGFDNCVMAQQMFGGHGFIEQWGMSQFVRDARIAMIYEGANGIQALD
                     LVGRKLPRNGGKALMTFMGEVGAFLEANAGDEAMKAYCAPLKTSLGHLQAASMWLMQN
                     GMANPDNAGAASVDYLHLFGLVALGYMWALQAKVASEKLKAGAGPAMSAKLTVGKFYM
                     ERVLPETAARLAMLQSGAATMMSLPAEAF"
     gene            complement(5835..6272)
                     /locus_tag="PLQ11_09245"
     CDS             complement(5835..6272)
                     /locus_tag="PLQ11_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRK25124.1"
                     /translation="MRAMSQSSAFSTSAVRTAATPTASPAPEAREWTISDMSRHFGLT
                     LRALRFYESKGLIKPNRFGGARYYTARDRVRLNLILTAKQMGFTLTETAALIGKLGDD
                     ESAELPLTSKTVQTQIAFLEDQRSAIDRALNQLYARRDGQASA"
     gene            6502..>6982
                     /locus_tag="PLQ11_09250"
     CDS             6502..>6982
                     /locus_tag="PLQ11_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25125.1"
                     /translation="MDGQPWSVRGVEPDIRADAFEAARKSGLTLGQWLNGVIRDSLVD
                     LKATRGASGWSDPVRTLQESTAIYPAPGQGPAPMPFQPEAARATMHNYPGQQPAYPYA
                     PMPPGYAPPAPPAPPQVLYPAAMPYAPPPPPPYVAGYPGAYQPAPYPGPFAGPYPQPA
                     "
BASE COUNT         1229 a         2249 c         2194 g         1310 t
ORIGIN      
        1 aggctaacct ctgtgccggt cgcagccatg ccggaacagc gcttgaagat cctctccgcc
       61 acctgtcagc gcaacgaagg cccgttcctg ctggaatggc tggcctatca ccggctgatc
      121 ggcgtcaccg atttcgtggt gttctccaac gattgcgagg atggatcgga ccatctgctg
      181 accaccctgc agggccatgg cctgcttctg gaaatcggcc ggggcgaggc tggcactatc
      241 cttacgcagc cttcttgccg gcctgccggc cgtagaaggt ctcgccccgg gcggccatgt
      301 cacgcaattg cgggttcacc gcaaagcgat tgccgtgctt cttggccaag gcatcacaca
      361 aggcgacgaa cgccttcgtc cccatcccgt cgatgaagga gatcgccccg ccggtgaacg
      421 gcgcaaagcc gaaaccgagg atcgagccga catccgcctc gcgcacgtcg gtgatgacgt
      481 tctcttccac cgtgcgcgcc gcctccagcg cctggatggc gagcaggcgg tccttcatct
      541 cctgcacgtc gatggtctcc gggtccttca gcctgggctg aagatccctg agcccggccc
      601 acagccgctt ctggcccttg ccttctggat agtcgtagaa gcccttgccg ttcttgcggc
      661 cgagacgccc gcgcttgacc accatctctt ccagaagcgc ctcctgcttg ggatcgacgg
      721 ccttcggccc gagatccttc ttggttgcct gcaggatctt ccagccgaga tcgagcgcca
      781 cctcgtccat cagcgacagc gggccgaccg gcatgccggc catcttggcc actgtctcga
      841 tcatcggcgc cggaacgcct tccatcagca tcagctgacc ttcgcggatg tagttgagca
      901 cgcagcggtt ggcgaagaag ccgcgcgtat cgttgacgac gatcggggtc ttcttgatga
      961 tgcggacaaa gtccagcgcc gtggcgatcg cccggtcgcc tgtcctgccg cccttgatca
     1021 cttccaccag cagcatcttg tcaaccggcg agaagaagtg gatgccgacg aactggtccg
     1081 gccgcgacga ggtctcggcc agcgaggtga tcggcagcgt cgaggtgttg gaggcaaaga
     1141 tcacatcctt gccgatcacg gcctcgacct tggcgatgac atcggccttg acctgccggt
     1201 cctcgaacac tgcctcgacg atgagatcga catccttgag cgcggcataa tcgggggtcg
     1261 gatggatgcg tccgagcacc gcatcgcgat gcgcggtggt ggcgcggccc ttcatcacct
     1321 gatccgacag cagcttctgg ctgaacgcct tgcccttgtc ggcggcttcc tgatcgcggt
     1381 cgatcagcac cacctcaagc ccggccagcg ccgagacata ggtgatgcca gcgcccatga
     1441 acccggcacc gacgacgccg atcttcctga tcgaggtcgg cgccacgccc gccggacgcc
     1501 gcgcgccctt ctccagttct cccttggaga cgaacagcga gcggatcatc atcgccgctt
     1561 ccttggagcg gatgatcttg gcgaagtagc ggctctcgac cctgagcgcg agatccatcg
     1621 gcagttgcag cccctcgtag accacctgga ggatggcccg tgcggcggga taattgtcga
     1681 aggtctcgcg ccggtagatg gcattggccg gcggccagat catcatgccc tgcgcggtat
     1741 gcaccttgtt cgacggcagg cgataggtct cggtgtccca cggcgccttg gccgaaccgc
     1801 cgccacgaat ccaggccttg gccgcctcga ccagcttgtc ctgtggcacg acctcgtgga
     1861 tcagccccat gccgcgcgcc atcagaccct tgatctggtc gccgcggaac aagagttgca
     1921 gcgcatcgcc cgattgcatc agccgggcaa cgcgctgtgt gccgccggcg ccggggaaca
     1981 gccccacctt gatctccggc agtccgacct tggtcttgtc gctgtccgag gcgatgcggt
     2041 gatggcagga cagcgccagt tcgaacgcgc cgccgaggca gacgccgtga atggcggcga
     2101 cgaacggctt gccgcaggtc tccagcttgc ggaacagctg cgacagccgc gccgtttcgg
     2161 tcagcagacg ggcattcgcc ttgtcctcgc cgtcggtctt gagcatcttc tggtagaagg
     2221 cgccaatcga ctggaggaag ttgaggtcgg cgccgccgga gaatgaatcg ggcttgcccg
     2281 agacgatgac gcagcctttc agcttggcat cgccggcaac ggtgtcgatg acggaatcga
     2341 gttccttcat ccactcctcg ttgatgacgt tcatggtctt tccggggctg tcccaggtca
     2401 gggtgacaag gccctcggcg tcggcgtcga agcggaaatg ggtgagtttc atgacaaaat
     2461 cctctcagac gcgttcgaca atcatggccg agcccatgcc atgggcaacg cacagcgtga
     2521 ccaggccgac cgccttgttg cgccgctcaa gctcgtcgat caccgtgccc aggatcatgc
     2581 cgccggtagc ccccagcgga tggcccatgg cgatggcgcc gccattgacg ttgacgatgt
     2641 ccggatcaat gtcgagcgat tgcagatagc gcagcaccac cgaggagaag gcctcgttga
     2701 tctcgatgag atcgatgtcc ttgaggctca tcttcgtgtt cttgaacagc ttcagcgtca
     2761 catcggtcgg cccggtcagc gtcagcgcca gatccgagcc gagcgaggca aagccgacga
     2821 ttcgggcgcg cggggtaagg cccgccttct tgcccgccgc cttgctgccg accagcaccg
     2881 ccgaggcgcc gtcaacgatg ccggaagagt tgccggcatg atgcatgtgc tcgatgaact
     2941 cgacatccgg atggccctgg atcgccaccg cgtcgaagcc gccctgttct cccatcatca
     3001 cgaacgaggg cttcagcgcg ccgagcgcct gcatgttggc ctcgggccgc acggtctcgt
     3061 cacggtcgag tatggtgatg ccgttgacgt ccttcaccgg gatcaccgat ttggcaaacc
     3121 ggccctcggc ccaggctttc gccgccttgt ggtggctgtc cacggcgaac tggtcgcact
     3181 gggtgcgggt gaacccgtat ttggtggaga tcagatccgc cgccacgccc tgcggcgaga
     3241 aatacgacgg gatcgcgaca tccggatccg ccgccggcag catgcccgag gagcccatgc
     3301 cgatacggct catcgattcg atgccaccgc cgatcgccat ctgcttctgc ccggccatga
     3361 cctgtgcggc ggcaaaggcc accgcatcga gacccgaggc acagaagcgg ttgatctgca
     3421 cgcccggcac ggtcttgcca tagccggccg cgatcgccgc cgaacgggcg atgacagcgg
     3481 aggattcgcc caccggatcg acgcagccga gcaccacatc ctcgaccagc gtcggatcga
     3541 gattgttgcg ctctttcagc gccttcagcg gtgccaccgc cagcttgagg gcggtgacct
     3601 catgcaggga gccatcggcc ttgcccttgc cgcgcggcgt tcggacgtga tcgtagatga
     3661 aggcgtcggt catggagctg tgtccttcgc gaggagtgat atgcgtttta caatagcgcg
     3721 gaacgtggcc aagtcatgca tcgcggtgag cggcggcgga tcagaacgcc tccgccggca
     3781 ggctcatcat ggtggcggcg cccgattgca gcatggcaag gcgcgctgcc gtctccggca
     3841 ggacccgctc catgtagaat ttgcccaccg tcaacttggc ggacatggcg gggccggcgc
     3901 cggccttgag cttttcgctc gccacctttg cctgcagcgc ccacatatag ccgagcgcga
     3961 ccagcccgaa caggtggagg taatcgaccg aagcggcgcc ggcattgtcg gggttggcca
     4021 ttccgttctg catcagccac atcgaggctg cctgaaggtg gccgaggctg gtcttgagcg
     4081 gcgcgcaata ggctttcatc gcctcatcgc cggcattggc ctcaaggaac gcgccgacct
     4141 cgcccatgaa ggtcatcagc gccttgcctc cgttgcgcgg cagcttgcgg ccgaccagat
     4201 cgagcgcctg gatgccgttg gcgccctcgt agatcatggc gatgcgggca tcgcgcacga
     4261 actggctcat gccccattgc tcgatgaaac catggccgcc gaacatctgc tgggccatca
     4321 cgcaattgtc gaagccgcga tcggtgagga acgccttcag caccggcgtc agcaggccga
     4381 gcaactcgtc ggagtccgcg cgcgcctttt cgtcgccgcc gcgatgggcg acgtcgctct
     4441 tgagcgcgcc ccacagcatg aaggcacggg cgccctcgtt gaaggagcgg atggtgagca
     4501 gcgtgcggcg cacatccgga tgaacgatga tcggatcggc cgccttttcc gggttcttga
     4561 cgccggtcag cgagcggccc tggatgcgtt cgcgcgcata ggcgacggcg ttctgatagg
     4621 caacttccga ttgcgcgagg ccctggatgg cgaccccgag ccgggcctcg ttcatcatca
     4681 cgaacatcgc gttgaggccc ttgttggcct cgccgatgag gaagccggtc gctccgtcat
     4741 aattcatcac gcaggtcgag ttggcgtgta tgcccatctt ttcctcgatc gagccgcagg
     4801 ccacggcatt gcgcgcgccg ggattgccat tcgcgtcggg gacgaatttc ggcacgatga
     4861 acagcgaaat gcccttcacg ccctccggag cgccctcgat gcgggcgagc accagatgga
     4921 tgatgttctc ggtgatgtcc tgctcgccag ccgagatgaa gatcttggtg ccggtgatct
     4981 tgtagcttcc gtccgcctgc ggagccgcct tggtgcgcat aaggccgaga tccgtgccac
     5041 aatgcggctc ggtcaggttc atcgtgccgg tccatgtgcc ggcgatcatc ttgggcagat
     5101 aggtagcctt gagttcgggc gagccgtgaa cgtcaatggc cgcgatggcg cctgcggtaa
     5161 ggcccggata catcgagaac gacatgttgg cggcagatgc aaactcgttg acgatcgctg
     5221 ccatcgtgta aggcagtccc tgtccgccat actctgggtt gcaggcgaga ctcagccagc
     5281 cgctgtcgac ataggccttg taggcgtcct tgaagccctt cggcgtggtg acgctgccat
     5341 ccgggtggcg gacgcaacct tcctgatcgc ccgacatgtt gagcggctgc agctgctcct
     5401 cgcacagttt ggcaatttca ccgaggatgg cgtcgagcat ctccggcgag agatcggaaa
     5461 agccgggaag attgctgtag cgtccgatgt ggaacacatc gttgagcagg aatgcgacgt
     5521 cttcgacggg ggccttgtag atcggcatca tgtcctccgg acgcgactgg ccgatgcatt
     5581 ggccagtccg gtagctctcc aatgatcggc gcagggccga cgatagttca catataaact
     5641 atatgctcga ctttgaaaag agggcgcgca gctttttgtg ctggcacacc cattgccggg
     5701 cgaggcggag agaggcgagc agccgatggg gcagctccgg cggctcgcat tcggtttccc
     5761 gagtgtctca aaaaaatgcc gcgccatccc ggggtgggag gcgcgggcga gggggccttc
     5821 ggggagaaac gaaatcaggc ggacgcttgc ccgtcacgcc gggcatacaa ctggttcaaa
     5881 gcgcgatcga ttgcacttcg ctgatcctcc agaaaggcga tctgggtctg gacggtcttg
     5941 gaagtcagcg gcagttcggc gctttcgtcg tcccccagct tgccaatcaa tgcagcggtt
     6001 tcggtgaggg tgaatcccat ctgcttggcg gtcaggatca ggttgagccg cacccggtca
     6061 cgagcggtgt agtagcgtgc gccgccaaac cggttcggtt tgatcagtcc cttggattcg
     6121 tagaaacgaa gggcccgcag ggtcagcccg aagtgccgcg acatgtccga gatggtccat
     6181 tcccgcgctt cgggcgcggg gctggcagtc ggcgtcgcgg ctgtcctgac cgctgatgtc
     6241 gagaatgcgg atgactggct catggcacgc atcaattcct ccaggggcat cgaatcagtg
     6301 caacaatcat aggaagcaaa aatcagaaac acaacctaga gttacatcga tcttttttaa
     6361 ccattgccgc cccgaaaagg cacaggcctt aaccagtcgt ttaccaatgg cggcaaagtc
     6421 tttcctgtga tggcaatgga atgccggggg accgattcgg ccggaaaagc tgcgtcgctc
     6481 aaccaggcat agcggggtaa gatggacggt cagccgtgga gcgtcagagg ggttgagccg
     6541 gacatccggg ccgatgcctt tgaggctgcg cggaaatccg gtttgacgct cgggcagtgg
     6601 ctcaacggcg tcatccgtga ctctctcgtc gatctcaagg caacccgtgg cgccagcggc
     6661 tggagtgatc cggtacgcac gctgcaggag tccacggcca tctatccggc tccggggcag
     6721 gggccggctc ccatgccatt tcagccagaa gcggcgcgcg cgaccatgca caattatccc
     6781 ggccagcagc cagcctatcc ctacgctccc atgcctccgg gctatgctcc ccccgcgccg
     6841 cctgcgccac cgcaggtgct ctatcctgcg gcgatgccat atgctccgcc tccaccgcca
     6901 ccctacgtcg cgggctatcc gggggcctac cagcccgcgc cctatccggg cccgtttgcc
     6961 ggaccctatc cccagccggc tc
//
LOCUS       DAOMXG010000131         6873 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_5791_length_6873_cov_9.132884, whole genome shotgun sequence.
ACCESSION   DAOMXG010000131 DAOMXG010000000
VERSION     DAOMXG010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 6873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6873
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5791_length_6873_cov_9.132884"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            376..837
                     /locus_tag="PLQ11_09255"
     CDS             376..837
                     /locus_tag="PLQ11_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRK25126.1"
                     /translation="MVRKAYCLLLATAAMLAAGGAALASPESIRPLIARHAAANNVPF
                     ALADAVVRIESRYNPAARNRANLGLTQISHQTARGIGYRGAAAGLLSAETNLVWGMKY
                     LGMAYHMANGNICGTVMRYQSGLMATRMSGANRIYCGKVQAILGGKGLRAS"
     gene            999..1379
                     /gene="rnpB"
                     /locus_tag="PLQ11_09260"
     ncRNA           999..1379
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLQ11_09260"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1393..2457)
                     /locus_tag="PLQ11_09265"
     CDS             complement(1393..2457)
                     /locus_tag="PLQ11_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003501317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HRK25127.1"
                     /translation="MPPLAGLYVVDFTTLLPGPFASLMLAEAGAEILKIEPPRGDDMR
                     RFPPLVNGESAAFAALNAGKTFLRLDLKDPAAIAGLKPHLERADIILEQFRPGVMDRL
                     GLGYQTIRAFNPRVIYCSITGYGQTGPRANEAGHDLNYISRTGMLSLSTGGADKPTLP
                     PLLAADIAGGSFPAMINILLGLRQRDQTGEGCYIDIAMADTPASFAWHAAATLAVSGR
                     SPLDGAELFTGGSPRYHTYPTLDRRLVAVGALEDKFWNVFCDAIGVPELLRVPSAPPA
                     TVIEFISQIIATKPARHWKQVLEPLDACVCVVATLEEATTDPHFILRGLFSRRTKLAD
                     GTEVPCAHVPVAAAFRKKPR"
     gene            complement(2548..2820)
                     /locus_tag="PLQ11_09270"
     CDS             complement(2548..2820)
                     /locus_tag="PLQ11_09270"
                     /inference="COORDINATES: protein motif:HMM:NF017992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein usg"
                     /protein_id="HRK25128.1"
                     /translation="MTAASKSRPAKDGYGMTTAQILYRAPDFHHLVREHIWTGYDQAP
                     DYPALRRFLESWSRSLETSLLSVTITQAEEKPSAGTPGGSHFYLLN"
     gene            2958..4040
                     /locus_tag="PLQ11_09275"
     CDS             2958..4040
                     /locus_tag="PLQ11_09275"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRK25129.1"
                     /translation="MDAFRRPIFETKPTLFWAAVALLCGVIWWALGRVLAPFLVGLGL
                     AYVLNPLVVRLRHVGLGRITAASLVILIFFFGLGYLIYRLAPFLAMQGRDFIKALPET
                     LQRLQGILGSWRGAIEERLGIDLAAASSEISLGQITTTAMNWLTGSLTSVGATGQAVM
                     SSIEFLLIVPFAVFYFLIDWERLVRALQRLVPVSLRRDVFSLTAEIDQMLGGYLRGQA
                     LVGLFLGSFYALALWLTGLNYGLLIGAISGLVAFIPYLGTATCLVLAIGFGIVQFWPD
                     WTMLAIILGIVVAGQMIEGNVLTPLFVGRHVGLHPLTLMFGLMALGSLYGFVGLLLSV
                     PVTGALAIVIRRMVGRYRASDFFRKG"
     gene            4119..4898
                     /gene="scpB"
                     /locus_tag="PLQ11_09280"
     CDS             4119..4898
                     /gene="scpB"
                     /locus_tag="PLQ11_09280"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF008506.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HRK25130.1"
                     /translation="MAYVRTAFEDNIGTITLDHFEKRNALSEPLVHEITDALDDFQRK
                     RARVVVLRAMPGVKVWSAGHDVEELPEGRRDPLGWHDPLRHLIRSIENCTAPVIAMIE
                     GGVWGGACETVMACDIIIAAPNATFAVTPAKLGVPYNVSGMLTFLNSAPLRIIKEMVF
                     TAKPVSAERAMAMELINHLVPLEELEGFTYGMARDIAANAPLSISVMKEQLRILAGAH
                     MMSPQAFERVQGLRRIVYDSADYAEGIRAFKEKRKPVYRGE"
     gene            complement(4905..5714)
                     /locus_tag="PLQ11_09285"
     CDS             complement(4905..5714)
                     /locus_tag="PLQ11_09285"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HRK25131.1"
                     /translation="MTKPLSGRTALVTGSTSGIGLAIAEALAGAGARIGLNGLGTPEQ
                     IAAAEAAVRKAGAPEVRFFDANLMDPAAVDRMMDQALGWGGGIDILVNNAGMQHTASI
                     ATMPVEKWQAIIALNLSACFYTMRRALPAMAARGFGRVINIASVHGLVASIDKAPYVA
                     SKFGLVGMTKVAALEYAAAGSRDSGGVTVNAICPGWVETALIEPQIVARSERFGGDRI
                     KGVQDLVGEKEPSRRMALPSDIGALALFLCSSAAHNITGTAMPVDGGWTAQ"
     gene            6073..>6873
                     /gene="rsmH"
                     /locus_tag="PLQ11_09290"
     CDS             6073..>6873
                     /gene="rsmH"
                     /locus_tag="PLQ11_09290"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRK25132.1"
                     /translation="MRHIPVLRSEALAALAPVPGGVYVDGTFGAGGYSRALLALHPDI
                     RVIAIDRDPAAIAGGAELVAASGGRLSLIQGCFGDLDTLVRDSGVGSVDGVVLDIGVS
                     SMQIDEALRGFSFRADGPLDMRMSSEGDSAAEIVNHADEAEIADILYHFGEERLSRPI
                     ARAIVGERKVRPFETTRQLADLIGRVVRSRPGDIHPATRSFQALRIAVNDELGELVRA
                     LHGAERLLKPGGRLAVVSFHSLEDRIVKVFLAARSGRGGGSRHAPVPVT"
BASE COUNT         1160 a         2116 c         2352 g         1245 t
ORIGIN      
        1 ttgccggtgg caaggccgcc gatggccgcg ccggttgccg cgccgatcgc cgcgccaccc
       61 agtgcgcgat cgcgtggatc attcggattg caggcgccga gtgctccggc aaccaatcca
      121 atggcggcga cgctcatcag tttcttcatg ggtctctcct tctggcgaac caacgtgacg
      181 ttcaaggcaa gagcaatcgg tgatctgagt caagcggacg gcaggatcta tcaagacgcc
      241 agtgaacggg atgtaaatgg cgctggtgtg gcaccacgca caccgatcgc catgatcttg
      301 ccatggatca gcccggaaat cgccacccga ttggatgatc gggcctgccg acgagacaac
      361 gaatggaatg atcgaatggt tcgcaaagcc tattgcctgc tgctcgcaac ggccgcgatg
      421 ctggctgccg gcggtgccgc gctggcctcg ccagagagta tccggccgct gattgcccgc
      481 catgcggcgg ccaacaatgt gccgttcgcg ctcgccgatg cggtggtgcg catcgaaagc
      541 cgctacaatc cggcggcgcg caaccgcgcc aatctcggcc tgacccagat cagccaccag
      601 acggcgcgcg gcatcggcta tcgcggcgct gctgcggggt tgctcagcgc cgagaccaac
      661 ctcgtctggg gcatgaaata tcttggcatg gcctatcaca tggccaacgg aaacatctgc
      721 ggcaccgtga tgcgctacca gagcggcctc atggcaacgc ggatgtccgg cgccaaccgc
      781 atctattgcg gcaaggtgca ggcgatcctg ggcggcaagg gtctcagggc ctcctgagcc
      841 cagatcacgc tctcgttctg attgaatcat caaaacgaga gataacgtga tcgattacaa
      901 tagtttatag catgctttcc gcgaaaaacc gttgccactt tttcgcagca tgctctatcg
      961 cttgacgctg gcgcccggat tgcccacaag cagtcgggcc agtcggccgg gcggccgctc
     1021 ccgcaaggga gaggaaagtc cgggctccac ggaagaacgg tgccggataa cgtccggcgg
     1081 gggcgacccc agggacagtg ccacagagaa cgaaccgctc cggcatgccg gagcaagggt
     1141 gaaaaggtgc ggtaagagcg caccgcgcga ccggcaacgg gagcggcatg gcaaaccccg
     1201 ccgggagcaa gaccgaatag ggacgacgag ggcggggaaa cccgtccgga ccctcttccg
     1261 ggttgtcgtc cgggttggtt gcatgaggcg gctggcaaca gtcgtcccag aggaatggcc
     1321 gccacgtcgc cggttcgccg gcggccctac agaacccggc ttacaggccg gctggcattt
     1381 catccaggtc gatcagcgcg gtttcttgcg aaacgctgcg gcaacgggca catgcgcgca
     1441 gggcacttcc gtgccgtccg ccagctttgt gcggcgggag aacaggccgc gcaggatgaa
     1501 atgcggatcg gtggtggctt cttcgagcgt cgcaaccacg cagacgcagg catcaagcgg
     1561 ttcgagaacc tgcttccagt gccgcgccgg cttggtggcg atgatctggc tgatgaactc
     1621 gatcaccgtt gccggtggtg cggagggaac gcgcaacagt tcggggacgc cgatggcatc
     1681 acaaaagacg ttccagaact tgtcttccag cgcccccacc gcgaccagcc ggcggtcgag
     1741 cgtcgggtag gtatgatacc ggggcgagcc gccggtgaac aattcggccc catcgagggg
     1801 cgaacggccc gagacggcaa gcgtggccgc ggcgtgccag gcgaaggacg ccggcgtatc
     1861 ggccatggcg atgtcgatat agcagccttc accggtctgg tcgcgctggc gcagcccgag
     1921 caggatgttg atcatggccg ggaaggaacc gccggcaatg tcggcggcca gcaaaggcgg
     1981 cagggtgggc ttgtcagcgc cgccggtcga aagcgacagc attccggtgc gggagatgta
     2041 gttgaggtcg tgcccggcct cgtttgcgcg cggaccggtc tggccgtagc cggtgatcga
     2101 gcaatagata acgcggggat tgaacgcacg gatcgtctgg tagccaagcc cgagccgatc
     2161 catcacgccg gggcggaact gctccaggat gatgtcggcg cgctcaagat gcggcttcag
     2221 gccggcgatg gcggcaggat ccttgaggtc gagcctgagg aaggtcttgc cggcgttcag
     2281 cgcggcaaag gctgccgatt cgccgttcac cagtggcgga aaacggcgca tgtcgtcgcc
     2341 gcgcggcggt tcgatcttga ggatctcggc tcctgcctcg gccagcatca gcgacgcaaa
     2401 agggccgggc agaagggtgg tgaaatcgac gacgtagagg ccagcgaggg ggggcatgag
     2461 tgaggctcca caatggcccc cagcctgcga ctgttcgcag gctttgtcta cagacagctt
     2521 gtcgccggtg gaggttggcc tgtcggttca gttgagcagg tagaagtggc tgccgccggg
     2581 cgttccggcg gagggtttct cctcggcctg ggtaatggtc accgacagca gcgaggtttc
     2641 cagcgaccgg gaccaggatt ccagaaatct gcgcagggcc ggataatcgg gcgcctgatc
     2701 atatccggtc cagatatgct cgcggacgag atgatgaaaa tcaggggccc gatagaggat
     2761 ctgggcggtg gtcatcccat agccatcttt ggcggggcgc gacttcgacg cggcggtcat
     2821 gcaactctcc cttggtgctg ggccatcggg gacttcatgc gcgatcggat catcctcgac
     2881 cccgtttgtt aacaaagcct tcagcgcggg cgcggtgcgg cgcttgccag cccgcccaaa
     2941 gccaatatgg tcctgccatg gatgcgtttc gccggccgat tttcgaaacc aagccgaccc
     3001 tgttctgggc agcggtggcg ctgctctgcg gagtcatctg gtgggcgctg gggcgggtgc
     3061 tggcgccgtt tctggttggg ctggggcttg cctatgtgct caatccgctg gtggtgcggc
     3121 tgcggcatgt cgggctcggc cgcatcaccg ccgccagtct ggtgattctc atcttcttct
     3181 tcggcctggg ctacctgatc taccggctgg cgccgtttct ggcgatgcag gggcgagact
     3241 tcatcaaggc gctgccggag acgctgcaac ggcttcaggg gatcttggga agctggaggg
     3301 gcgccatcga ggaacgcctc gggatcgatc ttgccgccgc ttcgagcgag atttcgctcg
     3361 ggcagatcac aacgacggcg atgaactggc tgaccggttc gctgacctcc gtgggggcaa
     3421 ccgggcaggc ggtgatgagt tccatcgaat tcctgctgat cgtgccgttc gccgtgttct
     3481 atttcctgat cgactgggag cggctggtgc gcgcgctgca acggcttgtg ccggtctcgc
     3541 tgaggcgcga cgtgttctcg ctgaccgccg agatcgacca gatgctcggc ggctatctgc
     3601 gcggacaggc gcttgtcggg ctgtttctcg gcagcttcta cgccctggcg ctgtggctca
     3661 ccgggttgaa ctacggattg ctgatcggcg cgatttccgg tcttgtggcc tttatcccct
     3721 acctcggcac ggcaacctgt ctggtgctgg cgatcggctt cggcatcgtg cagttctggc
     3781 cggactggac gatgctggcg atcattctcg gcatcgtcgt cgcggggcag atgatcgagg
     3841 gcaatgtgct gacgccgctg ttcgtcgggc gccatgtcgg gttgcatccg ctgacgctga
     3901 tgttcggcct gatggcactc ggcagcctct acggctttgt cgggctgctg ctttcggtgc
     3961 cggtgaccgg agcgctggcc atcgtcatcc ggcgcatggt cgggcgttat cgggcgagcg
     4021 atttcttccg caagggatga ccgaacgtcg cgctgggcaa ccttccttgc caagcgccgt
     4081 ttttcgttat tttctctcgg gaaaaccggg aggaagccat ggcctacgtg cgcacagcgt
     4141 tcgaggataa tatcggcacg atcaccctcg atcatttcga aaagcgcaat gccctttccg
     4201 aaccgctggt gcatgagatc actgacgcgc tggacgactt ccagagaaag cgggcacggg
     4261 tggtggtgct gagggcgatg ccgggcgtca aggtctggtc cgccggccat gatgtcgagg
     4321 aactgcccga aggccggcgc gatccgctcg gctggcatga tccgttgcgc cacctgatcc
     4381 gttcgataga gaactgcacc gcgccggtca tcgcgatgat cgagggcggc gtctggggcg
     4441 gtgcctgcga gacggtgatg gcctgcgata tcatcatcgc tgcgccgaat gccacattcg
     4501 ccgtgacgcc ggccaagctc ggcgtaccct acaacgtgtc gggcatgctg accttcctca
     4561 acagcgctcc gctgcgcatc atcaaggaaa tggtctttac cgccaagccg gtcagcgccg
     4621 agcgggcgat ggccatggag ctcatcaacc acctggtgcc gctcgaggaa ctggaaggtt
     4681 tcacctatgg catggcgcgc gacatcgcgg ccaatgcgcc gctgtcgatc tcggttatga
     4741 aggagcagct gcgcattctc gccggcgccc atatgatgag tccgcaggcg ttcgagcggg
     4801 tgcagggcct caggcgcatc gtctatgaca gcgccgatta cgccgaaggc atccgcgcct
     4861 tcaaggaaaa gcgcaagccg gtctatcgcg gggaataggc gcctttactg cgccgtccag
     4921 ccaccatcga ccggcatcgc cgtgccggtg atgttgtgcg cggcggaaga gcagaggaac
     4981 agcgccagcg cgccgatgtc ggagggcagc gccatgcggc ggctcggctc cttctcgccg
     5041 acgagatcct gcacaccctt gatgcggtcg ccgccgaaac gctcggagcg ggccacgatc
     5101 tgcggttcga tcagtgcggt ttcaacccag ccggggcaga tggcattgac agtcacgccg
     5161 ccgctgtcgc ggctgccggc ggccgcatat tccagcgccg cgaccttggt catgccgaca
     5221 agaccgaact tggaggcgac atagggcgcc ttgtcgatgg aggcgacgag gccgtggacc
     5281 gaggcgatgt tgatgacgcg gccgaagccc cgggccgcca tcgccggcag cgcgcggcgc
     5341 atggtgtaga agcaggccga gaggttcagt gcgatgatcg cctgccactt ctccaccggc
     5401 atggtggcga tggaggcggt gtgctgcatg ccggcgttgt tgaccagaat gtcaatgccg
     5461 ccgccccagc cgagtgcctg atccatcatc cggtcgacgg cggcggggtc catcagattg
     5521 gcgtcgaaga acctgacttc cggcgcgccg gccttgcgca cggcggcttc ggcggcggcg
     5581 atctgctccg gcgttccgag cccgttgagg ccaatacggg cgccagcccc ggccagtgct
     5641 tcagcaatgg caagaccgat gccgctggtc gagccggtga caagggcggt gcggccggaa
     5701 aggggcttgg tcatggcggg tgctccgggt gcgaaaacgg tgtcttccgc taagcccggc
     5761 gatggccggc gtcaactgcc ggcgatgccg caaccctgtc ccggcaaggc tttggtaacc
     5821 gttctttagg gttaacggct tctaatgccg gttccaatgg ccttctgcgc ccaaagcgcc
     5881 gggggcgttc cttgaagccc gttacgcccc atgatatcgc acatcatccc aagccagaca
     5941 ggaccgcgcc ttgccggtgt cgcgacgttg cgtcgcgcgc gtcgcgctgt gctgcgtcgg
     6001 cgcccgggcc ggcgaattcc gggcaaagga gggcggatga gcctgagcga gcagaacggc
     6061 gcggcggcgg ccatgcggca cattccggtg ttgcgcagcg aggcgcttgc ggcgctcgcg
     6121 cccgttcccg gcggcgttta tgttgacggc accttcggcg ccggcggcta cagccgggcg
     6181 ctgctggcgc tgcatcccga tatccgtgtc atcgccattg accgtgatcc ggccgcgatc
     6241 gctggcgggg cggaactggt ggcggcgtcc ggcgggcggc tgagcctcat ccagggttgc
     6301 tttggcgatc tcgacacatt ggtccgcgat tcgggcgttg ggtcggtcga cggcgtggtg
     6361 ctcgacatcg gcgtttcctc tatgcagatc gatgaggccc tgcgcggctt ttccttccgc
     6421 gccgacggcc cgctcgacat gcgcatgagc agcgagggcg acagcgccgc cgaaatcgtc
     6481 aatcacgccg acgaggcgga gatcgccgac attctctatc atttcggcga ggagcggctg
     6541 tcgcgtccga tcgcccgcgc catcgtcggc gagcgcaagg ttcggccgtt tgaaacgact
     6601 cgccagcttg ccgacctgat cggccgcgtg gtgcgcagcc gaccgggcga catccatccg
     6661 gcaacgcgga gcttccaggc actgcggatc gcggtcaacg acgaactcgg cgaattggtg
     6721 cgggcgctgc atggcgccga acgcctgctc aagcccggcg gccggcttgc ggtcgtcagt
     6781 ttccactcgc tcgaagaccg catcgtcaag gtgttcctcg ccgcccggtc tggccggggc
     6841 ggcggctcac gccatgcgcc ggtgccggtg acg
//
LOCUS       DAOMXG010000132         6670 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_6048_length_6670_cov_9.737415, whole genome shotgun sequence.
ACCESSION   DAOMXG010000132 DAOMXG010000000
VERSION     DAOMXG010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 6670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6670
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6048_length_6670_cov_9.737415"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(246..884)
                     /locus_tag="PLQ11_09295"
     CDS             complement(246..884)
                     /locus_tag="PLQ11_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012651590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRK25133.1"
                     /translation="MDLVLYTHPMSRGRIARWMLEEIGVPYRAEIVDYGPPMKGPAYR
                     AINPMGKVPALKHGDTIITETAAICTYLADAFPAAGLAPAPGSAARGPYYRWMFFAAG
                     PLEQAVTARSMGWEVPQNRAGMVGFGSYKTALDALETALAHGGYIAGDRFSAADVYVG
                     AQIGWGLQFGSIEKRDVFVAYWNLLVDRPAHRKAEATDNALLPQHLVPGMPG"
     gene            complement(986..2035)
                     /locus_tag="PLQ11_09300"
     CDS             complement(986..2035)
                     /locus_tag="PLQ11_09300"
                     /inference="COORDINATES: protein motif:HMM:NF022515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2865 domain-containing protein"
                     /protein_id="HRK25134.1"
                     /translation="MSGADLVRDFRNRPHGSSSPAWRKRSLIISAAVSIAAFGSIVVR
                     ANDDSSVYAVARHYNSAVRSAVRSVPLPQVFYPAPRQVVPTSLSYAPVFHELMPSGPI
                     NGTAKPAPKGSPKAIKVSPKALSAEDFAEGYLESRTSYCVRTCDGFFFPVGTPDSSDL
                     GAHEAACTRACPGAETAVYVAPSGSKGIEDATNRKGQRYEALATAFNHRTQYDSACSC
                     TNASKPRNYSVLTDFTLRKGDLVMGSNGLQQFNGAERFPLRRNDFARADMSKLNAAER
                     RNLQATEAASLRGSNSDRLSPSLKARIAEQVRLGSNSRDTAPGTQTAALRTVPGQNGR
                     DLRYVGPDRDFDRAR"
     gene            complement(2182..5460)
                     /locus_tag="PLQ11_09305"
     CDS             complement(2182..5460)
                     /locus_tag="PLQ11_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014331411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRK25135.1"
                     /translation="MSLSELSIRRPVMTTLLTLSFIIFGFFAFKKLPVAALPRVDFPT
                     INVQTNLPGASPETMASSVAAPLERQFATIAGITSMTSVSAQGATNITLQFDLDRNID
                     GAALDVQSAISATLRRLPTELPAPPSFRKVNPADQPILFMTLTSDTLPLSSVHEYAEN
                     VLQQQISQILGVAQVQIFGAQKYAVRVFFNPDALTVRGLTAQDLRTAVANANSNSPVG
                     QLRSERQRVTLEATGQIPRAADYNDLVIASRGGGQQAVKLSDVARAVDSVENDQTAAW
                     YNGTRAVMIAVFRQSDANTVETVERIRKQLPVYTSQLPPSVKLVVMNDRSLPIKEAVE
                     DVEFTLMLSVVLVILVIFLFLKRLSATVIPTLALPVSLVGTFAFMYLFGYSLDNISLL
                     ALTLAVGFVVDDAIVMLENIVRHIENGERPFEAALVGSKEIGFTILSITFSLIAVFIP
                     VFFMSGVVGRIFREFAATISVAILVSGFVSLTLTPMLCARFLKEETHHRKPSLFDRIV
                     DGGFNAILTFYRVTLDFSLRHRLIVLVLTFGSAWWAMHLYASIPKGFFPIEDTGLLRA
                     ATEGAPDTSFEAMSARHQQLIEIVRKDPAVAAVAGNVGGFSAVNQGFMFITLKPRAER
                     DEAQKIIGRLRAATSQVPGITFVAQPVQNLNLNAGRQSRSMYQYTLSGPNLQDLIDFA
                     PGHLARLQGLPQLRDVALDLQLRNPQVRIDIDREQAQRLGVSVAQIKSSLYNTFGGGT
                     ISTIYTSSSDFPVILEADRAFQNSPDALSRLYIRSQNTATAPAIGTATQGAVAAQQNA
                     FQVQNIPLDAFVRMTPSIGPLAVNRVSQMPAVTISFNTAPGVSLGDAVEAIGRLERDS
                     VLPQNVTTRFAGSAQLFQEALAGQDMLLIAAVLVIYILLGVLYESFIHPITILSGLPS
                     AGLGALLALKYYNMDLSVIAIIGILMLIGIVKKNAIMMVDFAIERRRAGVEALEAIRE
                     ACLIRFRPILMTTLAAALGAVPIAIGAGAGAELRQPLGIAVVGGLAVSQVMTLYITPV
                     IYYYLDKVDTFFAGHSHLGPLENEIAVQDALHVIEHPVEEPAQAAEGSAVTPKTSAAA
                     PATR"
     gene            complement(5609..>6670)
                     /locus_tag="PLQ11_09310"
     CDS             complement(5609..>6670)
                     /locus_tag="PLQ11_09310"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRK25136.1"
                     /translation="GGAGGRPTPVAIAEVVRKTMPYRVESIGTVQTVASVTVRSRVDS
                     QIEEILFADGSNVKAGDLLARLDSRVIEAQIRQGEATVLKDRASLDLARATLKRAEEL
                     AAGNFATKQRLDEARASVSQQEASLRASEASLEVLRTQLTFYTIRAPITGKAGLANIK
                     PGNMARSSDGAIAITTINQIAPIYVSFSLPQRYLQDIRKALAAGEGLVTATPQGGTTS
                     ATGRLELVENAMDNATGTIGLRALFDNKDELLWPGLIVSLKIVLREDKDVIVVPREAV
                     QMSQRGNFVFIVKDGVAKMQPVTVGRSVDNETIVLTGLEGQEQVIVDGQLLVVNGGPV
                     QPRPARSSQTGDASPKRAL"
BASE COUNT         1291 a         2019 c         2193 g         1167 t
ORIGIN      
        1 cccccgaaca gcggatgcat gccgaagttg ggcagcgccc ggaacagcac gcgggaagta
       61 aaatcggcgg gtttgaagcc gcgtcatacc agcggccctg acaattgacc gtcaggggca
      121 attgtcaggc ggctggttag gagcgaatgc gacgccgagc agggccgaag gcccggttgc
      181 gagggacggc ccgagaaatg acggacaggt catttgtgag ggccactggt atcagtccct
      241 ctgcttcagc ccggcatgcc cggaacgaga tgctgcggca gcagcgcatt gtcggtggcc
      301 tcggccttgc gatgggcggg gcggtcaacc agcaagttcc agtaggccac gaaaacatca
      361 cgcttttcga tggagccgaa ctgaaggccc cagccgatct gcgcgccgac atagacatcg
      421 gccgccgaaa agcgatctcc ggcgatgtag ccgccatggg ccagggcggt ctccagcgcg
      481 tcgagcgcag tcttgtacga gccgaatccc accatgccgg cccggttttg cggcacctcc
      541 cagcccatgg agcgggcggt cacagcctgt tcgagcgggc cggcggcaaa gaacatccag
      601 cgataatagg ggccgcgcgc agcgctgccg ggggcagggg ccagcccggc ggcgggaaag
      661 gcatcagcca gataggtgca gatagccgcc gtttcggtga tgatcgtgtc gccatgcttg
      721 agcgccggca ccttgcccat cggattgatc gcccggtagg ctgggccttt catcggcggg
      781 ccgtagtcga caatttcggc gcgatagggg acgccgatct cctccagcat ccaacgagcg
      841 atgcggccgc gcgacatcgg gtgcgtgtat aagacaagat ccatgactgc cctcgttgtt
      901 gttcctgaaa tgttctctat atcagtcggg ggtatctggc aagtgccgat ggttgatcgg
      961 tgcaaacgag gcttctgcgc ccggctcaac gggcgcggtc gaaatcccga tccggtccga
     1021 cgtagcgcag atcgcgcccg ttctgtccgg ggacggtgcg cagcgcggcc gtctgcgtgc
     1081 cgggcgcggt gtccctggaa ttcgagccga ggcgcacctg ttcggcgatc cgcgccttca
     1141 ggctgggcga gagacggtcg ctgttgctgc cgcgaaggct cgccgcttcc gtggcctgca
     1201 gattgcggcg ttcggcggca ttcagcttgg acatgtcggc ccgcgcaaaa tcgttccggc
     1261 gcagcgggaa ccgctcggcg ccgttgaact gctggaggcc attgctgccc atcaccagat
     1321 cgcctttgcg cagggtgaaa tcggtcaata ccgaatagtt gcgcggtttg ctggcattgg
     1381 tgcaggaaca ggcgctgtcg tattgcgtgc gatggttgaa ggcggtcgcc agcgcctcgt
     1441 aacgctgccc cttgcgattg gtggcatcct cgatgccctt ggaacccgaa ggggcgacat
     1501 agacggcggt ttccgcgccc gggcaggcgc gggtgcaagc ggcttcatgc gcgccaagat
     1561 cggaggaatc cggtgtgccg accgggaaaa agaagccgtc gcaggtgcgc acgcaataag
     1621 aggtgcgcga ttccagatag ccttcggcaa aatcctcggc gctgagcgcc ttgggcgaga
     1681 ccttgatggc tttcggcgat cccttgggcg ccggcttggc cgttccgttg atcgggccac
     1741 tcggcatcaa ttcatgaaac accggcgcat aactgagcga tgtcggcacc acctgacgtg
     1801 gcgccggata gaaaacctgc ggaagcggca ccgaacgaac ggcagagcgg accgcgctgt
     1861 tatagtgccg ggcaaccgca tagacgctgg aatcgtcgtt ggcgcgaacc acaatcgagc
     1921 cgaaagccgc gatgctgact gcggcgctga tgatcaggct gcgcttgcgc caggcggggg
     1981 aggacgatcc atgcggccgg ttgcgaaaat cacgcacaag atcggcaccg gacatgacac
     2041 aactccctca cccacacgga aggttaccaa aaagtaaaca cccttccagc gcgcgttgta
     2101 tcacaatggt gcagcgctca caacagagcc gctgcgccgt catgcgtaat ggttacttgg
     2161 gcgaaattat cggcgaggcg ctcagcgcgt cgccggggcg gcagcggagg tcttgggcgt
     2221 cacggccgat ccttcggccg cctgtgccgg ctcttcgacc ggatgttcga tgacgtgcaa
     2281 ggcatcctgc acggcaatct cgttctccag cgggcccaaa tggctgtggc cggcaaagaa
     2341 cgtatccacc ttgtcgaggt aatagtagat gaccggcgtg atgtagagtg tcatcacctg
     2401 cgacacggca agaccgccga caacggcgat gccaagcggc tgacgcaact cggcgccggc
     2461 gccggcgccg atggcaatcg gcaccgcgcc cagcgccgcg gccagggtcg tcatgaggat
     2521 cggccggaag cggatcaggc aggcttcgcg gatcgcttcc agcgcttcca cgccggcccg
     2581 tcgccgctcg atggcgaaat cgaccatcat gatggcattc ttcttgacga tgccgatcag
     2641 cattagaatg ccgatgatgg caatgacgct gaggtccata ttgtaatatt tcagcgcgag
     2701 cagcgcgccc agtccggccg aaggcaggcc ggacagaatg gtgatcgggt ggatgaagct
     2761 ctcatagagc acgccaagca ggatatagat gaccagcacg gcggcgatca gcaacatgtc
     2821 ctgaccggcc aatgcctcct ggaacagttg cgccgagccg gcgaagcggg tcgtcacatt
     2881 ttgcggcagt accgagtcgc gttcgagcct gccgattgcc tcgaccgcat cgccaagcga
     2941 aacacccggc gcggtgttga acgagatggt caccgccggc atctgcgaga cccggttgac
     3001 ggcgagcggg ccgatggacg gggtcatcct gacgaaagca tcaagcggga tgttctgcac
     3061 ctggaaggcg ttctgctgcg cggcaaccgc gccctgggtg gcggtgccga tggccggggc
     3121 ggtggcggta ttctggctgc ggatatagag ccgcgacagc gcatcggggc tgttctggaa
     3181 ggcgcggtcc gcctcaagga taacgggaaa atcggacgag gaggtgtaga tggtcgagat
     3241 cgtgccgccg ccgaaggtgt tgtagagcga ggacttgatc tgcgccacgg agacgccgag
     3301 tcgctgggcc tgttcacggt cgatgtcgat gcgcacctgc ggattgcgca attgcaggtc
     3361 gagcgcgacg tcgcgcagct gcggcagccc ttgcagccgg gcgagatgtc cgggcgcgaa
     3421 atcgatcaga tcctgcaggt tcggaccgga cagggtgtat tgatacatcg aacgcgactg
     3481 gcggccggca ttcagattga ggttctgcac cggctgggca acgaaggtga tccccggaac
     3541 ctggctggtg gcggcgcgca ggcggccgat gatcttctgc gcctcgtcgc gttcggcgcg
     3601 cggcttcagc gtgatgaaca tgaagccctg gttgaccgcc gaaaacccgc cgacattgcc
     3661 ggcaacggcg gccacggccg ggtccttgcg gacaatctcg atcagttgct gatggcgtgc
     3721 cgacatcgcc tcgaacgagg tatccggcgc tccttccgtt gcggcgcgca gcaagccggt
     3781 atcctcgatc gggaagaaac ccttggggat cgacgcataa agatgcatcg cccaccaggc
     3841 cgagccgaag gtcagcacca gcacgatcag gcgatggcgc agcgagaaat ccagcgtcac
     3901 ccgatagaag gtcagaatgg cgttgaagcc gccatcgacg atgcggtcga acagcgacgg
     3961 tttcctgtgg tgggtctctt ccttgaggaa acgcgcacag agcatcggcg tcagcgttag
     4021 cgagacgaag ccggacacca ggatcgccac cgagattgtc gcggcaaatt cgcggaagat
     4081 gcgtcccacc acgccggaca tgaagaacac cgggatgaac accgcgatca acgagaaggt
     4141 gatcgacagg atggtgaagc cgatctcctt ggagccgacc agcgccgcct cgaacgggcg
     4201 ttcgccgttc tcgatatggc ggacgatgtt ttcgagcatg acgatggcat cgtcgaccac
     4261 gaaaccaacc gccagcgtca gcgccagcag cgaaatgttg tccagcgaat agccgaacag
     4321 atacatgaag gcgaaggtgc caaccagcga gaccggcagc gccagcgtcg ggatcaccgt
     4381 cgccgacagc cgcttgagga acaggaagat caccaggatg accagaacca ccgacagcat
     4441 cagggtgaat tcgacatctt ccaccgcttc cttgatcggc agcgagcggt cgttcatcac
     4501 caccagtttc accgacggcg gcagctgcga ggtatagacc ggcaactgct tgcggatgcg
     4561 ctccaccgtc tcgaccgtgt tggcgtcgga ctgacggaac accgcgatca tcaccgcgcg
     4621 ggtgccgtta taccaggcag cggtctgatc gttctcgacc gagtcgaccg cccgggccac
     4681 atcgctcagc ttgaccgcct gctggccgcc gccgcgcgag gcaatcacca ggtcgttgta
     4741 atcggcggcg cgcggtatct ggcccgtcgc ttccagcgtc acccgctggc gctctgaacg
     4801 gagctggccc accggcgaat tggaattggc gttggccacc gcagtgcgca ggtcctgcgc
     4861 cgtcaggccg cgcacggtca gggcatcggg gttgaagaac acgcgcacgg catatttctg
     4921 cgcgccgaag atctgcacct gagcgacgcc gaggatctgc gaaatctgct gctgcagcac
     4981 attctcggca tattcgtgca cgctggacag cggcagggta tccgaggtca gcgtcatgaa
     5041 caggatcggc tggtccgccg ggttgacctt gcggaaactg ggcggggccg gcagttctgt
     5101 cggcaggcgc cgcagcgtcg cgctgatcgc cgactgcaca tcgagggcgg cgccatcgat
     5161 gttgcggtca agatcgaact gaagcgtgat gttggtcgcc ccttgcgccg aaaccgacgt
     5221 catcgacgtg atgccagcga tggtggcgaa ctgccgttcc aatggggctg caaccgagga
     5281 agccatggtt tccgggctgg cgccgggaag attggtctgc acgttgatgg tcggaaaatc
     5341 gacccggggc aaagcggcga ccggcagctt cttgaaagcg aagaacccga aaatgatgaa
     5401 cgacagcgtg agcaacgtcg tcatcaccgg ccggcggatg gacagttcgg acaaggacat
     5461 tgttgttgtt tccccccgct cttgctgccg aacctagagc atgctgcgaa aaagtgtgaa
     5521 cggtttttcg cgataagcat gctctaagct attcttatcg atcacgttat ctctcgtttc
     5581 ggtggttcaa ccgaaacgag agcgtgatct aaagtgcccg tttcggtgac gcatcgcctg
     5641 tctgggatga acgtgccggg cgtggctgca ccggaccgcc gttgaccacc agaagctgac
     5701 cgtcaacgat cacctgttcc tgcccttcaa gaccggtcag gacgatggtc tcgttatcga
     5761 cgctgcgccc aaccgtaacc ggctgcattt tcgcaacgcc atccttgacg ataaagacga
     5821 aattgccgcg ctggctcatc tgaacggctt cacgcgggac gacgatgaca tccttgtcct
     5881 cgcgcagcac gatcttgagc gacacgatca ggccgggcca cagcaattcg tccttgttgt
     5941 cgaacagggc gcgcaggccg atcgtcccgg tggcattgtc catggcgttc tcgaccagtt
     6001 cgagccggcc ggtggcgctg gtggttcccc cttgcggggt tgcggtgacc agtccctcgc
     6061 ccgcagcaag cgccttgcgg atatcctgca gatagcgctg cggcaggctg aagctgacat
     6121 agatcggcgc gatctggttg atcgtggtga tggcaatcgc cccgtcggag gaccgcgcca
     6181 tattgccggg cttgatattg gccaggcccg ccttgccggt gatcggcgcg cggatggtgt
     6241 agaaggtcaa ttgcgtcctg agcacttcaa ggctggcctc gctggcccgc aggctggcct
     6301 cctgctgcga gaccgaggcg cgcgcttcgt ccagccgctg cttggtggcg aaattgccgg
     6361 cggcaagctc ctcggcgcgc ttgagtgtgg cgcgggcgag gtcgaggctt gcccgatcct
     6421 tcagcaccgt cgcctccccc tggcggatct gtgcctcgat cacccgggaa tcgaggcggg
     6481 ccagcaagtc gccagccttc acattcgagc catcggcaaa caggatctcc tcgatctggc
     6541 tgtcaacccg ggagcgcaca gtgaccgagg caacggtctg aacagtgccg atggactcga
     6601 cacgataggg catcgtcttg cgcacaacct cggcaatggc caccggggtc ggccgtccgc
     6661 cggccccgcc
//
LOCUS       DAOMXG010000133         6662 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_6062_length_6662_cov_10.225064, whole genome shotgun sequence.
ACCESSION   DAOMXG010000133 DAOMXG010000000
VERSION     DAOMXG010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 6662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6662
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6062_length_6662_cov_10.225064"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="PLQ11_09315"
     CDS             complement(<1..353)
                     /locus_tag="PLQ11_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25137.1"
                     /translation="MRGLKAMGHHDLRHSAQAIWLGLALTLGTAVQAGEIYDPDLGQI
                     LVEPPEFEVIVPSDRTEFSEPGRPGVPSGQQDPAGVASSPDMTRPDRVPPAIMQPVAP
                     PVEPPTSPPRQAGPPP"
     gene            complement(440..1927)
                     /locus_tag="PLQ11_09320"
     CDS             complement(440..1927)
                     /locus_tag="PLQ11_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRK25138.1"
                     /translation="MTATILVVDDDPIQRRLLSEMVKRFGYDPVAVESGVGALEKLQS
                     GLDVAAMVLDLVMPDLDGMGVLARLRDMDDAPPVIVQTAHGSIETVISAMRAGAVDFV
                     VKPVGAERLQVSIKNAMRVEALEGEVRRLAKRSSGALQFRDLAARSSDMIRAVRLGER
                     AAKSQIPVLLEGESGVGKEVFARAIQGASDRRGKAFVTVNCGAIPGNLVESILFGHEK
                     GAFTGANERHAGKFVEASGGTLFLDEVSELPLEAQVKLLRAIQEGEVDPVGGRRTVKV
                     DIRLISASNRNLLDLVRQGKFREDLFYRLNVFPITIPPLRNRREEIPELARRFIARVS
                     AEEGGTVRAISPAALELLQRYDWPGNVRQLENAMFRAVVLSESDELTVDEFPQIAREV
                     EGFEARIPPVAAPAPRSERVEREIVRVELRDPSALTLLDGSGDVRKLDDVEGEAIRFA
                     IDHYRGQMSKIARKLGIGRSTLYRKLRELGLDDLADGESAETDAA"
     gene            complement(2135..4081)
                     /gene="acs"
                     /locus_tag="PLQ11_09325"
     CDS             complement(2135..4081)
                     /gene="acs"
                     /locus_tag="PLQ11_09325"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015822921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HRK25139.1"
                     /translation="MSERIVQVSKAWEKRAYVNDAKYKKMYAASIKDPEKFWGEQGKR
                     IDWFKPYTKIKNTSYKPGKVSIKWFEDGTTNVSYNCIDRHLATRGDQVAIIWEGDDPG
                     VSQKITYKKLHEEVCRFANVLKANGVKKGDRVTIYLPMIPAAAYAMLACARIGAIHSI
                     VFGGFSPDSIANRVEDCDSQIVITADEGLRGGKHVHLKKNVDVADSKVKGGIKKVIVV
                     KHTGGDIPMQAGRDVWYHEEAAKVSAHCPPVEMKAEDPLFILYTSGSTGKPKGVLHTT
                     GGYLVYASMTHQYVFDYKDGDIYWCTADVGWVTGHSYIVYGPLANGATTLMFEGVPSY
                     PDNGRFWQVVDKHKVNTFYTAPTAIRSLMGSGEELVKKYKRKSLRLLGSVGEPINPEA
                     WEWYYRVVGDSKCPIVDTWWQTETGGILITPLPGATALKPGSATRPFFGVQPMIVDAE
                     GKELKGATEGNLVITDAWPGQMRTIYGDHKRFEETYFSTYKGMYFTGDGCRRDKDGYY
                     WITGRVDDVINVSGHRMGTAEVESALVSHPKVSEAAVVGYPHDLKGQGIYAYVTLMAG
                     EKGTPDLRKELVAWVRKEIGPIASPDLIQFAPGLPKTRSGKIMRRILRKIAEDDFGSL
                     GDTTTLADPTVVDDLISNRQNKKA"
     gene            complement(4254..4661)
                     /locus_tag="PLQ11_09330"
     CDS             complement(4254..4661)
                     /locus_tag="PLQ11_09330"
                     /inference="COORDINATES: protein motif:HMM:NF020157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HRK25140.1"
                     /translation="MIYGGVNYLAVLVATVAGMASGAVWYGALARPWQSAAGLTPERI
                     AAAGGQAGKYALAALAKFVIAFCLAKLLRVLDAVSIGNSFALALFLWLGFVAMPMIVN
                     HRFQLHPWRLTFIDGGYWLLVLALQGLVIGFFG"
     gene            complement(4746..5159)
                     /locus_tag="PLQ11_09335"
     CDS             complement(4746..5159)
                     /locus_tag="PLQ11_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HRK25141.1"
                     /translation="MAHWLFKSEPFKWSWDQQVEAGPAGTFWNGVRNHLAKQNMMKMR
                     CGDTGFFYHSNEGKEIVGIVEVIRQFYPDHTDETGKFGMVDVRAVRKLVRPVSLAEVK
                     ATPALAGMALLTSARLSVQPVTDGEWRTILAMAEG"
     gene            complement(5167..5703)
                     /locus_tag="PLQ11_09340"
     CDS             complement(5167..5703)
                     /locus_tag="PLQ11_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020094933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRK25142.1"
                     /translation="MRALRRLLLPVLVVLSATYAWVRMAPPPQPVVEGPAPARASHVV
                     IHKSERLLTLFAGDRAFRTYRIALGGAPSGAKRQEGDGRTPEGRYHIDAANPRSAYHL
                     SLRISYPDRKDRAAAASRSVAPGGDIMIHGLPNGYGAIGIAHRLVDWTNGCIALTNGE
                     MDEIWRLVPVGTPVEILP"
     gene            complement(5705..>6662)
                     /locus_tag="PLQ11_09345"
     CDS             complement(5705..>6662)
                     /locus_tag="PLQ11_09345"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012252468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HRK25143.1"
                     /translation="GAFGTALAAVAARAGRPVLLYGRDAGVIDEIRRHRTHSRALPGL
                     TLPDGIAATDDLAQAASAAVLILAVPTQALRAAATSLAPHVARGVPVIAAAKGIELAT
                     GRFVTDILGEVLADAESAILSGPSFAADIVSGLPTAVTLAAHDGRRAEQLAAAVSGPA
                     FRIYHSDDVIGVAVGGAAKNVLAIAAGIVIGAGLGESARAALIARGFAELGRLATALG
                     ARRETLMGLSGLGDLVLTAASPQSRNFSFGLRLGEGRPPAELLAGGKLAEGAATAPAL
                     ASLARRHGVEMPISEAVAAVLDGRLSVAAAIQALLARPSRGE"
BASE COUNT         1240 a         2113 c         2148 g         1161 t
ORIGIN      
        1 ggcggcggcc ctgcctggcg gggcggagag gttggtggtt cgaccggggg ggcgacaggc
       61 tgcataattg ccggcggcac cctgtccggt cgggtcatgt ccggactgct tgcgaccccg
      121 gccggatcct gctggccgga cggcacgccc gggcggccag gctcggaaaa ttcggtgcgg
      181 tcagagggaa cgatcacctc gaactcgggc ggctcgacga ggatctggcc cagatccggg
      241 tcatagatct cgcccgcctg aacagcggtc ccgagcgtca aggccagacc cagccagatg
      301 gcttgcgccg agtgccgcaa atcgtggtgc cccattgcct tcagtccgcg catggtctcg
      361 aatcagcaga ttatcgcatg atcacgagag tgcctgaatc ggcccgttgt gtgacgatgg
      421 agtcgccagt cttgtgctgt caggccgcgt cggtttcggc ggattcgccg tcggcaaggt
      481 catccagacc cagttcgcgc aacttgcgat aaagcgtcga acggccgatg ccgagcttgc
      541 gggcgatttt cgacatctgg ccgcgataat gatcgatcgc gaagcggatg gcctcgccct
      601 ccacatcatc cagcttgcgc acatcgccgg agccatccag cagcgtcagc gccgaagggt
      661 cgcgaagttc gacacggacg atctcgcgtt ccacacgctc ggatcgcggc gcaggcgccg
      721 ccaccggcgg gatccgggcc tcgaagcctt ccacttcacg ggcgatctgc ggaaattcgt
      781 cgaccgtgag ttcgtccgat tcgctcagga caacggcgcg gaacatggcg ttctccagct
      841 ggcgcacgtt gccgggccag tcatagcgtt gcagaagctc gagcgctgcg ggcgaaatgg
      901 cgcggacagt gccaccttcc tcggcggaga cgcgggcgat gaagcggcgg gccagttcgg
      961 gaatttcctc gcgtcggttg cgcagcggcg gaatggtgat ggggaaaacg ttgaggcggt
     1021 agaacaggtc ctctcggaac ttgccctgcc ggacaagatc aagcaggttg cggttcgagg
     1081 ccgagatcag gcgaatgtcg actttcaccg tgcggcgacc gccgacggga tcgacctcgc
     1141 cctcctgaat ggcgcgcagc agcttgacct gcgcctcaag cggcaactcg ctcacctcgt
     1201 cgaggaacag ggtgccgccg gatgcttcga cgaactttcc ggcgtggcgt tcgttggcgc
     1261 cagtgaacgc ccccttttcg tggccgaaca ggatactctc gaccagattg ccgggaatgg
     1321 cgccgcaatt gacggtgacg aaggccttgc cgcgccggtc ggatgcgcct tggatggcgc
     1381 gggcaaacac ttccttgccg acgccggatt cgccttcaag cagcaccgga atctgtgact
     1441 tggcagcgcg ctcgccgagg cggacagcgc ggatcatgtc ggaggatctg gcggcaaggt
     1501 cgcggaattg cagcgcgccg gacgagcgct tggccagacg ccgcacctcg ccttccaggg
     1561 cctcaacgcg catggcgttc ttgatcgata cctgcaagcg ctcggcgccg accggcttga
     1621 caacgaaatc gacggcgccg gcgcgcatgg ccgaaatcac cgtttcaatg gaaccgtggg
     1681 cggtctggac gatgaccggc ggggcatcgt ccatgtcgcg cagccgggca agcacgccca
     1741 tgccatcaag atcgggcatc accaggtcga gcaccatggc ggcgacatcg aggccggact
     1801 ggagcttttc gagcgcgcca acgccgcttt ccaccgcaac cgggtcatag ccgaatcgct
     1861 tgaccatctc ggagagcagg cgccgctgaa tcggatcatc atcgacgacc agaatggttg
     1921 cagtcataca gaaagtcccc aatgcccagg gacagcgaag cactgtcctc ttttgatgca
     1981 gtgtgccggg gttccgtaaa caggctgtaa atcatactga aaaaatccgg tccctgatcg
     2041 caatggcgga tggccagccc gtctgccgcc aaacggcaac ggccgcggag gatatccgcg
     2101 gccgtcgcaa tcgctatccg gaattttgaa agccttacgc tttcttgttc tggcggttgg
     2161 agatcagatc gtcgacgacg gtcggatcgg ccagcgtcgt ggtatcgccc agagagccga
     2221 aatcgtcctc ggcgatcttg cgcaggatgc ggcgcatgat cttgcccgag cgagtcttgg
     2281 gcaggccggg cgcgaactgg atcagatcgg gcgaggcaat cggaccaatc tccttgcgca
     2341 cccaggctac cagctccttg cgcaggtccg gcgtgccctt ctcgccggcc atcagggtga
     2401 cataggcata gatgccctgg cccttgagat catgcggata accgacaacc gccgcctccg
     2461 aaaccttcgg gtgtgagacg agggccgatt caacctcggc cgtgcccata cggtggcccg
     2521 agacgttgat cacatcgtcg acgcggccgg tgatccagta atagccgtcc ttgtcacgac
     2581 ggcagccgtc accggtgaaa tacatgccct tgtaggtcga gaaataggtt tcctcgaagc
     2641 gcttgtggtc gccgtaaatc gtgcgcatct ggcccggcca ggcgtcggtg atgacgaggt
     2701 tgccctcggt cgcgcccttg agttccttgc cttcggcgtc gacgatcatc ggctgcacgc
     2761 cgaagaaggg ccgcgtggcc gagcccggct tcagggcggt ggcgcccggc agcggggtga
     2821 tcaggatgcc gccggtctca gtctgccacc aggtgtcgac gatcgggcac ttcgaatcgc
     2881 cgacgacgcg gtaataccac tcccaggctt ccggattgat cggctcgccg accgaaccca
     2941 acagacgcag agacttgcgc ttgtatttct tgaccagctc ttcgcccgag cccatcagcg
     3001 agcggatcgc ggtgggggcg gtgtagaagg tgttgacctt gtgcttgtcg accacctgcc
     3061 agaagcggcc gttgtcggga tagctcggca cgccctcgaa catcagcgtg gtggcgccgt
     3121 tggcgagcgg accgtagaca atgtagctgt ggcccgtgac ccagccgacg tcggcggtgc
     3181 accagtagat gtcgccatcc ttgtaatcga agacatattg atgggtcatc gaggcataga
     3241 cgagatagcc gccggtggtg tgcagcacgc ccttcggctt gccggtggag cccgaggtgt
     3301 agaggatgaa cagcgggtct tccgccttca tctcgaccgg cgggcaatgg gccgagacct
     3361 tcgccgcttc ctcgtgatac cagacgtcgc gaccggcctg catcgggatg tcgccgccgg
     3421 tatgcttgac gacgatcacc ttcttgatgc cgcccttgac cttgctgtca gccacatcga
     3481 cgttcttctt caggtgaacg tgcttgccac cgcgcaggcc ctcatcagcg gtgatgacga
     3541 tctggctgtc gcaatcctcg acgcggttgg cgatcgagtc cggagaaaaa ccgccgaaaa
     3601 cgatcgagtg gatcgcgccg atgcgggcgc aggccagcat ggcataggca gccgccggga
     3661 tcatcggcag gtagatggtg acgcggtcgc ccttcttgac tccattggcc ttcagcacgt
     3721 tggcaaagcg gcagacttcc tcatgcagct tcttgtaggt gatcttctgc gaaacgccgg
     3781 ggtcatcgcc ttcccagatg atcgcgacct gatcgccgcg cgtcgcaaga tggcggtcga
     3841 tgcagttgta ggagacgttg gtggtgccgt cctcgaacca tttgatcgac accttgcccg
     3901 gcttgtagga ggtgttcttg atcttggtgt acggcttgaa ccaatcaatc cgcttgccct
     3961 gctcgcccca gaatttctcc gggtccttga tcgaggcagc gtacatcttc ttgtatttgg
     4021 cgtcgttcac ataggcccgc ttttcccaag ccttggatac ctgcacaatt ctctcggaca
     4081 ttgacgcctc cagttccgtt tcctgatcct gcggctgtgc agacagaccg ataagtcagc
     4141 gctgccaaac cattcctctt tcgattatcg caaaccgggc aacggaaaaa gccggcccgc
     4201 cctcacactt tggttgcgag aagaaaggtg aatcggaccg cagaatgcgc atgtcagccg
     4261 aaaaagccga tcaccagacc ctgcaacgcc agcaccagca gccaatagcc gccgtcgatg
     4321 aaggtcagcc gccagggatg aagctggaaa cgatggttga cgatcatcgg catggcgaca
     4381 aagccgagcc acaggaacag ggccagcgcg aaactgttgc cgatcgagac cgcatcgagc
     4441 acgcgcagca gcttggcaag gcagaaggcg atcacaaact tggccagtgc ggccagcgcg
     4501 tattttcccg cttgcccgcc ggctgcggcg atccgctccg gtgtcagccc ggcggccgac
     4561 tgccacgggc gggcgagtgc gccataccat accgcccctg aggccatgcc ggccacggtt
     4621 gcaaccagaa ctgccagata attgacgcca ccatagatca tctccgcctc ctgccgctga
     4681 acttcatgcc cccggtcttt gcacagcttc cgtccgggct ggatgccagt atgccggacg
     4741 gcgaactatc cctcggccat cgccagaata gtccgccatt cgccatccgt caccggctgc
     4801 accgacaggc gcgcgctggt cagcagcgcc attcctgcga gtgccggtgt ggctttcacc
     4861 tcggcaagac tgacagggcg gaccagcttg cgcaccgccc tgacatcgac catgccgaat
     4921 ttgccggtct cgtcggtatg atctgggtag aattgccgga tgacctcgac aatgccgacg
     4981 atctccttgc cctcgttgga atgatagaag aagccggtat cgccgcaccg cattttcatc
     5041 atgttctgct tggccagatg attgcgcacg ccgttccaga atgtgccggc ggggccggcc
     5101 tcgacctgct ggtcccacga ccacttgaat ggttcggatt tgaacagcca atgcgccatc
     5161 atcgcctcag ggaagaatct cgacgggggt tccaaccggc accagccgcc agatctcgtc
     5221 catctcgcca ttggtaagcg cgatgcaacc attggtccag tcgacaaggc gatgggcgat
     5281 gccgatggcg ccatagccgt tgggcaggcc gtggatcatg atgtcgccgc cgggagcgac
     5341 gctgcgggaa gccgctgcgg cacggtcctt gcggtccgga taggaaatgc gcagggacaa
     5401 gtgataagcg ctgcgtggat ttgccgcatc aatgtgatag cgcccctcgg gcgtacggcc
     5461 atccccttcc tgacgcttgg ctccggacgg cgcgccgccc agcgcgatcc gataggtccg
     5521 gaatgcccgg tccccggcaa aaagggtcag cagccgctct gatttgtgaa tgacgacgtg
     5581 gctggcccga gcaggagcgg gtccctcgac aacgggctgg ggcggaggcg ccatgcggac
     5641 ccaggcatat gtggcggaca gaaccacaag caccggcagc agcaggcggc gcagggcgcg
     5701 catgtcactc cccgcgcgaa ggccgggcaa gcagggcctg aatcgccgca gccaccgaaa
     5761 ggcgtccgtc aagcacggcg gcaacggctt ccgaaatcgg catttcgacc ccgtgcctgc
     5821 gtgccaggct cgccagcgcc ggagcagtcg cagccccttc ggcgagcttg ccgccggcga
     5881 gcagctcggc tggagggcgg ccttcaccga ggcgcaggcc gaaagagaag ttgcgtgatt
     5941 gcggcgaggc ggcggtcagc accagatcgc cgagaccgga gaggcccatc agggtctcgc
     6001 gccgggcgcc cagcgctgtg gcgagccggc cgagttcggc aaaaccgcgg gcgatcagcg
     6061 cagcacgggc actttcgccc aaacccgcgc cgataacgat gccggccgcg atggccagca
     6121 cgttcttggc cgcaccgccg acggcgacgc cgatcacatc gtcgctgtgg tagatgcgga
     6181 aggccggacc ggacaccgca gcggcgagct gctcggcccg ccggccatca tgggcggcca
     6241 aggttaccgc ggtcggcagg ccagaaacga tgtcggcagc aaagctgggg ccggagagaa
     6301 tggccgactc ggcatcagcc agcacttcgc ccaggatgtc ggtgacaaag cggccggtcg
     6361 ccagttcaat ccccttggcg gcggcaatga ccggaacacc cctggctaca tgcggcgcaa
     6421 ggctggttgc cgcagcgcgc agcgcctggg tcgggacggc tagtatcagc acggcagccg
     6481 atgcggcctg cgccagatcg tccgtagcgg cgatcccgtc gggcagggtg agacccggga
     6541 gggcgcggga atgggtgcgg tgacgccgga tctcgtcaat caccccggcg tcgcggccat
     6601 agagcagcac cggccgaccg gcgcgggccg cgactgccgc gagcgcggtg ccgaaagcgc
     6661 cg
//
LOCUS       DAOMXG010000134         6660 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_6065_length_6660_cov_10.128388, whole genome shotgun sequence.
ACCESSION   DAOMXG010000134 DAOMXG010000000
VERSION     DAOMXG010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 6660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6660
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6065_length_6660_cov_10.128388"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="PLQ11_09350"
     CDS             <1..694
                     /locus_tag="PLQ11_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRK25144.1"
                     /translation="EAGLDVDIRGHGPVDAAARLALVDCAERFDLARLAIHGETLVER
                     RPPLLGMGRAQVNAPPGGFLQATQAGEEILAELVRGHTGRARKVVDLFCGVGPFALRL
                     AETASVVAYDSDRAAIAALLKAARGTPGLKPVTAEARDLFRRPLLAPELNGHDVAVLD
                     PPRAGAEAQARLLGLSRVPKIVYVSCDTASFLRDARLLAGLGLTLDRVTPVDQFAFTP
                     HLELVGVFSRSR"
     gene            complement(678..1583)
                     /locus_tag="PLQ11_09355"
     CDS             complement(678..1583)
                     /locus_tag="PLQ11_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012066855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRK25145.1"
                     /translation="MLSRRSLLKMLTGALAGAFGFGGYAFGIEPAHRLTLARYRLRPD
                     RFGGLKLRIACFSDLHASEPQMGEARITAMVDLLNSLGADVILALGDYGMGQTFYARP
                     VPLTRVSGLLSRLRAPLGQYAVLGNHDYWGSSLKNWPYTRAKVNDTADGYRTMLREGG
                     FTLLENDVVRLSAAGRPFWLVGTGSSIALPIRASHFESFADLDGQLAKLTDDAPAILM
                     AHEPDLFVRVPGRIALTLSGHTHGGQVRLFGYSPHTPSNYGNRFAYGHIIEDDRHLIV
                     SGGLGTSVLPVRFGVPPEVLLIEIG"
     gene            complement(1603..1824)
                     /locus_tag="PLQ11_09360"
     CDS             complement(1603..1824)
                     /locus_tag="PLQ11_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25146.1"
                     /translation="MSLPFKLPSPRAPGVPQPVQDARYQLERLYREIGISAVASALHM
                     QQLASPEARAAAERASHEIPPMLRKENAA"
     gene            complement(1925..2374)
                     /locus_tag="PLQ11_09365"
     CDS             complement(1925..2374)
                     /locus_tag="PLQ11_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012454896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRK25147.1"
                     /translation="MSDPSSSSAMRVWFRLIRLHQALYSEVASRLRVIGLSVPQFDVL
                     STLTEREGISQRELADRLYVTKGNVSGLIDRLVEAGLVIRQSIPEDRRSHALHLTEEG
                     VRLAQLGMVEHTRFMAETLGRLPEADIATLDRILIDWRDVVRGLPSS"
     gene            2507..3274
                     /locus_tag="PLQ11_09370"
     CDS             2507..3274
                     /locus_tag="PLQ11_09370"
                     /inference="COORDINATES: protein motif:HMM:NF019032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HRK25148.1"
                     /translation="MRTYPVRTSILALWGRRLAMFGLAAAVAGVVLARLGRIAPVQGL
                     ILIGAGAALSALALGLALAAYREIWRTGAVGLRRANVAALLALLNLAVPAWQIVRAYS
                     LPAINDVSTDLKEPPAFSRARSALEARAGHVPPEPSEETRQLQEQAYSDLEPLTLELN
                     PEEAYGLVIEALNGLKWTIVDRSPPSGRTGIGRVDAIQRTPILRFSDDVTIRIRPLAN
                     VTRIDIRSASRIGRHDLGANAARIRTLIVAIQALTSN"
     gene            complement(3284..4675)
                     /gene="folK"
                     /locus_tag="PLQ11_09375"
     CDS             complement(3284..4675)
                     /gene="folK"
                     /locus_tag="PLQ11_09375"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HRK25149.1"
                     /translation="MTLATLSLGANLADKREAIAEAVERLRVVPGIRILARSADYRTP
                     PWGKTDQDWFVNAAVKIETSLAPEALLAACLAVEAAMGRKRAERWGPRRIDIDIISYG
                     ALTIDKPGLRLPHPHAAERAFVLVPLAEIAPDIAVGRTTAHQALARLDRTGIEPLPAV
                     APPEAPQFDRAFTDPPGVVTRLSPRVRRIVAPNPGPYTFTGTCTYIIGEGRVTVLDPG
                     PNDPAHIEAILATLGGETIERILVSHTHKDHSPGAAPLKALTDAPVEGCGPHRAARPL
                     HPGETTLEAGADRSYAPDRQIHDGERFGGAGYTLAALATPGHTANHLCFALEEDGVVF
                     SGDHVMGWSTTVVAPPDGSMRDFMASLEQLKMRPEALFLPGHGGPITQPQRFVRALIH
                     HRRMREAAILGQVRGGIGQIADLVPRIYENLNPALVGGAAMSILSHLEDLAERGLVVA
                     DGPATLKAHYRAS"
     gene            complement(4656..5036)
                     /gene="folB"
                     /locus_tag="PLQ11_09380"
     CDS             complement(4656..5036)
                     /gene="folB"
                     /locus_tag="PLQ11_09380"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HRK25150.1"
                     /translation="MSDRIRVTRIGVYAYHGVHSEERSLGQRFFVSLDCRLDLGPAGR
                     SDDVSKTADYSRMVEQVQEVAVARAFRTIEGLAEAVAAMCLADYPLLDSITVTVEKPG
                     APIAAMIDTVAVEITRSRHDAGNA"
     gene            complement(5046..5891)
                     /gene="folP"
                     /locus_tag="PLQ11_09385"
     CDS             complement(5046..5891)
                     /gene="folP"
                     /locus_tag="PLQ11_09385"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019965344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRK25151.1"
                     /translation="MTKIAKPVAQSRGWPALPTHGALLMGVLNVTPNSFSDGGVFAGK
                     EAALAQAQRIIDEGADILDIGGESTHPLAAPVDADEELARVRPVLEALSGQIALPLSI
                     DTYKASVAKVALELGAAIVNDVWGLQRDPAIADVAAEYGAGVCIMHNRTGFDPALDVI
                     GDIRRFFDRSLAIAERAGIRRDAIVLDPGIGFGKTFNQNLLVLNRLDLLMDYGLPLLI
                     GCSRKGFIGKITGQAVAAERLSGSLGAHVAAVMKGARIIRAHDIAAHREALAVVAAIG
                     RETTD"
     gene            6137..>6660
                     /locus_tag="PLQ11_09390"
     CDS             6137..>6660
                     /locus_tag="PLQ11_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRK25152.1"
                     /translation="MDQVECVVVGAGVVGLAIARELARAGREVIVLETADSIGTGTSS
                     RNSEVIHAGLYYPAGLIKARLCVEGKQRLYDFCAAYNVPHRRCGKLLVATTEAELPKL
                     HALRQQAEANGVTDLKPLTGAELRALEPALNAVAGMLSPSTGIIDSHAFMLALQGDAE
                     AHGAMLAFETPVIGG"
BASE COUNT         1147 a         2132 c         2227 g         1154 t
ORIGIN      
        1 cgaggcgggc ctggatgtcg acattcgcgg ccatggccct gtcgatgcgg ctgcccgtct
       61 ggcactggtc gattgcgccg agcgctttga tctggcccgc cttgccattc atggcgaaac
      121 gctggtggag cgtcgtccgc cattgcttgg aatggggcgg gctcaggtca atgccccgcc
      181 cggcggtttc cttcaggcga cgcaggcggg cgaagagatc ctggccgaac tggtgcgggg
      241 tcacactggc cgtgcgcgca aggttgtcga cctgttctgc ggagtcggcc cctttgccct
      301 ccggctggcg gaaacggcaa gcgttgtggc ctatgattcg gaccgggcgg cgatcgcggc
      361 attgctcaag gccgccagag gcacgccggg cctcaagccg gtgacagccg aggcgcgcga
      421 tctgttccgc cggccgctgc tggcgccgga actcaatggc catgatgttg ccgtgctcga
      481 tcccccgcgt gcaggcgctg aggcacaggc ccgtctgctc gggctctccc gcgtgcccaa
      541 gatcgtctat gtctcgtgtg atacggccag tttcctgcgc gatgcccggc tgctggccgg
      601 ccttggcctg acgctcgacc gcgtcacccc ggtcgatcag ttcgccttca cgccgcatct
      661 cgaactggtc ggcgtgttca gccgatctcg atgagcagga cttccggcgg cacgccgaag
      721 cggaccggca gcacgctggt gccaagcccg ccggaaacga tcaggtggcg atcatcctcg
      781 atgatgtggc cgtaggcgaa gcggttgcca tagttggacg gcgtatgcgg cgaatagccg
      841 aacagccgca cctgcccgcc atgggtatgg cccgacagcg tcagtgcaat gcggccgggc
      901 actctgacga acaggtccgg ctcatgcgcc atcaggatcg ccggggcgtc gtcggtcagt
      961 ttggcaagct gtccatcaag atcggcaaag ctttcaaaat gcgaggcccg gatcggcagg
     1021 gcgatcgacg atccagtgcc gaccagccag aagggccggc cggcggcgga gaggcgcaca
     1081 acgtcgttct ccagcaacgt gaagccgcct tcgcgcagca tggtgcggta accgtcggcg
     1141 gtgtcgttga ccttggcgcg ggtgtagggc cagttcttga ggcttgaccc ccagtagtca
     1201 tgattgccga gcacggcata ctggccgagc ggggccctga gcctgctgag cagcccgctg
     1261 acgcgcgtca gcggcaccgg ccgggcgtag aacgtctggc ccatgccgta atcgcccagc
     1321 gccaggatga catcggcgcc aaggctgttg agcaggtcga ccatcgccgt gatgcgggcc
     1381 tcgcccatct ggggttcgga ggcgtgcaga tcggaaaagc aggcgatccg gagtttgagc
     1441 ccgccgaagc ggtccggccg cagccgatag cgggccagtg tcagccggtg cgccggctcg
     1501 atgccgaacg catagccgcc aaagccgaag gcgcccgcaa gggcgcccgt cagcatcttc
     1561 aggagggaac gacgcgacag catgcaaccc gcccgcaacc gctcaggccg cgttttcctt
     1621 gcgcagcatg gggggaattt catggctcgc ccgctcggcc gcggcgcgcg cctctgggct
     1681 ggccagctgt tgcatgtgca gagccgaggc gaccgccgag atgccgattt cgcgatagag
     1741 cctctcaagc tggtagcggg catcctggac gggttgcggc acgccgggcg cacgcggaga
     1801 aggcagtttg aaggggaggg acatcggcct gacctctggt ctgtgccgcg cggatgtgat
     1861 gcgcagccac ccggtcaatg tctatcggca cggttacaaa tccgtaaaag ccggtggggc
     1921 aggatcagct cgacggcagg ccacggacca catcgcgcca gtcgatcagg atccggtcga
     1981 gcgtggcgat atcggcttcc ggcagccgac cgagtgtctc ggccatgaac ctagtatgct
     2041 cgaccatgcc caattgagcc agccgcacgc cttcctcggt cagatgcagg gcatgcgagc
     2101 gacgatcttc cgggattgat tgccggatca ccagaccagc ttcgaccagc cgatcgatca
     2161 gcccggagac attgcccttg gtgacataca gccggtctgc gagttcgcgc tgtgaaatgc
     2221 cctcgcgctc ggtcagcgtc gacaggacgt cgaactgcgg caccgacagg ccgatcacgc
     2281 gcaagcggga tgcaacttcg ctgtagagag cctgatgcag gcggatcaac ctgaaccaga
     2341 cgcgcatggc gctcgacgat gacggatcag acatcacacg actttcctga actttacgcc
     2401 tgataagcgg ctggctggtg acttgcaatg gttcgttgtc tggcacttga cgtttctgaa
     2461 ccgggcacgg catggaaagg atgtctcttg ctcaccgaga ctgctgatgc gcacttatcc
     2521 tgtccgcacc tcgatcctcg ccctgtgggg gcgacggctg gccatgtttg gccttgcggc
     2581 ggcggtggca ggtgtcgtgc tcgcgcggct cggacgtatt gccccggttc aggggttgat
     2641 cctgatcggt gccggagccg ccctgtcggc gctggcgctg gggctggcgc ttgcggccta
     2701 tcgggaaatc tggagaaccg gagccgttgg cctgcgccgg gccaatgtcg cggctcttct
     2761 ggcattgctc aatctggcag tgccggcctg gcagatcgtc cgggcgtata gtctgccggc
     2821 gatcaacgat gtttcgaccg atctgaagga accgccggcc ttttcgcgtg cccgctcggc
     2881 gctggaagcg cgggccggtc atgtgccgcc agaaccaagc gaggaaaccc gccagctcca
     2941 ggaacaggcc tattccgatc ttgagccttt gaccctggag ctgaacccgg aggaagccta
     3001 cggactggtg atcgaggcgc tgaacgggct taaatggacg attgttgacc ggtcgccccc
     3061 ctccggacgg accggcatcg gccgcgtcga tgcgatccag cggacgccga tcctgcggtt
     3121 cagtgatgat gtcaccatcc gtatccggcc gctcgccaac gtgacacgca tcgatatccg
     3181 ctccgcctcg cgtatcggcc gccatgatct tggcgccaat gccgcgcgta tccgcacctt
     3241 gatagtggca atccaggccc tgaccagcaa ttgacgccgg cgatcagctg gcccggtaat
     3301 gggccttcaa agttgcgggc ccatcggcca cgacaaggcc gcgttcggca aggtcttcca
     3361 gatgcgacag gatcgacatg gcggccccgc cgaccagcgc cggattgagg ttctcataaa
     3421 tccgcggcac cagatcggcg atctggccaa tgccgccgcg gacctgcccg agaatggctg
     3481 cttcgcgcat ccggcgatgg tggatcagcg cccggacgaa acgctgcggc tgggtgattg
     3541 gcccgccatg gccaggcagg aacaacgcct cgggccgcat tttcagctgc tccagcgagg
     3601 ccatgaaatc gcgcatcgag ccatccggcg gtgcgaccac cgtcgttgac cagcccatca
     3661 catggtcgcc ggagaacaca acgccatcct cctccagcgc aaagcacagg tggttggccg
     3721 tatgcccggg ggtggccagc gccgcaagcg tatatccggc gccgccgaac cgttcgccat
     3781 cgtggatctg ccggtccggc gcatagcttc ggtcggcgcc ggcctcgagc gtggtttcac
     3841 ccggatgcag cggccgtgca gcccggtgtg gaccgcagcc ttccaccggc gcatcggtca
     3901 gcgccttgag cggcgccgcg cccggtgaat ggtccttgtg ggtatggctg acgaggatgc
     3961 gttcgatcgt ttcaccgccg agcgtggcca ggatcgcctc gatatgggcg ggatcgttcg
     4021 ggccgggatc gagcacggtc acccgcccct cgccgatgat gtaggtgcag gtccccgtga
     4081 acgtataggg gccgggattg ggcgcaacga tccggcgcac gcgagggctg agccgggtaa
     4141 ccacccccgg gggatcggta aaggcgcggt cgaactgcgg cgcctccggt ggtgcaaccg
     4201 ccggcagagg ttcaattcct gtcctgtcga gccgggcgag cgcctgatgt gccgtggtcc
     4261 ggcccaccgc gatgtcgggg gcgatctcgg ccagcggaac cagcacgaag gcgcgttcgg
     4321 cggcatgggg atgtggcagc ctgagacccg gtttgtcgat tgtcagggca ccgtagctga
     4381 tgatgtcgat gtcgatgcgg cgtggccccc agcgttcggc gcgcttgcgg cccatcgccg
     4441 cctcgacagc aaggcaggcg gccagcagtg cctccggagc cagactggtt tcgatcttga
     4501 ccgccgcatt gacgaaccag tcctgatcgg tcttgcccca gggcggcgtg cggtaatccg
     4561 ccgagcgggc caggatgcgg atgccaggaa cgaccctgag ccgttccacc gcctcggcga
     4621 tggcctcgcg cttgtcggca agattggcgc cgaggctaag cgttgccagc gtcatggcgg
     4681 ctccgggtga tctcgacggc aacggtgtcg atcatcgcgg caatcggcgc accgggcttt
     4741 tcgaccgtga cggtaatgga gtcgagcagc ggataatcgg caaggcacat ggccgcaacc
     4801 gcctcggcca gcccttcgat ggtgcggaag gcgcgggcga ccgccacttc ctgcacctgc
     4861 tcgaccatcc gcgaataatc ggccgtcttg gaaacatcgt cgctgcgacc ggccggcccg
     4921 aggtcgagcc ggcaatcgag cgagacgaaa aagcgctggc caaggctgcg ctcttcggaa
     4981 tgcacaccat gataggcgta gacgccgatg cgcgtcaccc ggatgcggtc actcatgctg
     5041 tctcctcaat ccgtcgtctc gcgcccgatt gccgccacca cggccagggc ctcgcgatgg
     5101 gctgcaatat catgggcgcg gatgatgcgc gcgcctttca tgactgcggc aacatgggcg
     5161 ccaagtgatc cggacagccg ctctgctgcc acggcctgac cggtgatctt gccgatgaac
     5221 cccttgcgcg aacagccgat cagcagtggc aggccgtaat ccatcagcaa atcgagccgg
     5281 ttcagcacca gaaggttctg gttgaatgtc ttgccaaagc cgatgccggg gtcgagcacg
     5341 atcgcatcgc ggcgaatgcc cgcccgctcg gcgattgcca gcgaccgatc aaagaatcgg
     5401 cgaatgtcgc cgatgacatc cagcgcagga tcgaaccctg tccggttgtg catgatgcag
     5461 acaccggcgc catactcggc cgcgacatcg gcaatggccg gatcgcgctg gagaccccag
     5521 acatcgttga cgatggctgc gccgagttcg agcgcgacct tggccacaga agccttgtag
     5581 gtatcgatcg acagcggaag ggctatctgc cccgacagcg cttccagtac cgggcggacg
     5641 cgggccagtt cctcgtccgc atcgaccgga gctgccagcg gatgggtcga ttccccgccg
     5701 atatcgagaa tgtccgcgcc ttcgtcgatg atgcgctgcg cctgcgccag cgcggcctcc
     5761 ttgccggcaa acaccccgcc gtcggagaag gaattgggcg tgacgttgag aacccccatc
     5821 agcagtgcgc catgggtggg cagagccggc cagccgcgcg actgcgcgac aggttttgcg
     5881 attttcgtca tcgtcactcc ggccgatgcg tcaggccaac agcgtcctag agcatgctgc
     5941 gatcaagggg aagccggtta atcgcggcag gcatgctcta aacacttgat agcgatcacg
     6001 ttatctctcg ttttgatggg tcaatcaaag cgaaagcgtg atccagaatc atgacgtggt
     6061 ttccacaagc ccggcgcgcg gccttgtgct ttggccggag cggttctacg gctggcaatc
     6121 agaacagggg tggcggatgg atcaggtcga atgcgtggtt gtcggcgccg gggtggtcgg
     6181 cctggcgatc gcccgggaac tggcgcgcgc cggccgcgag gtgatcgttc tggaaaccgc
     6241 cgattcgatc ggcaccggta ccagctcgcg caattcggaa gtgatccatg ccgggctcta
     6301 ctatcccgcc ggtttgatca aggcgcggct ctgcgtcgag ggcaagcagc ggctctatga
     6361 cttctgcgcg gcctataacg tgccgcatcg ccgctgcggc aagctgctgg tagcaacgac
     6421 ggaggcggaa ctgcccaagc tgcatgcgct caggcagcag gccgaggcca acggcgtcac
     6481 cgacttgaag ccgctcaccg gcgccgagct cagggcgctg gagccggcgt tgaacgccgt
     6541 cgctggcatg ctgtcgccgt ccaccgggat catcgacagc cacgccttca tgctggccct
     6601 gcagggcgat gccgaagccc atggcgcgat gctggccttc gaaacgccgg tcatcggcgg
//
LOCUS       DAOMXG010000135         6637 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_6090_length_6637_cov_8.617290, whole genome shotgun sequence.
ACCESSION   DAOMXG010000135 DAOMXG010000000
VERSION     DAOMXG010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 6637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6637
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6090_length_6637_cov_8.617290"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..472
                     /gene="nusB"
                     /locus_tag="PLQ11_09395"
     CDS             8..472
                     /gene="nusB"
                     /locus_tag="PLQ11_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRK25153.1"
                     /translation="MTRGERRSAARLAAVQGLYQMDLTGKGILEVLGEFETHWIGKEI
                     EGDQYKPAERGLFRAIVQGVLAHQAPLDRACDRILADGWPLKRVEALMRAVLRAGAYE
                     LTHASDVPVKVVVSEYVDIAASFLDGEEVGMVNAVLDRLARETRAGELARSS"
     gene            487..1626
                     /gene="nhaA"
                     /locus_tag="PLQ11_09400"
     CDS             487..1626
                     /gene="nhaA"
                     /locus_tag="PLQ11_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008123317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HRK25154.1"
                     /translation="MRERLGSTIRMFLASEAAGGLVLMGAAALALIIANSPLSHAYFS
                     TLKAYLGGLSVLHWINDGLMAVFFLLVGLEIKREIISGELSTWGKRALPGLAALGGVV
                     VPALIFLAFNPPGSATSRGWAIPTATDIAFALGVLALLGPRVPASLKIFLAALAIIDD
                     LVAVLVIALFYTDSLSLPMLAGAGAVIAVLAVLNRRGVTALWPYLALGAVLWGLVLKS
                     GVHATIAGVALAMMVPLRTGDDHPPLIRLEHWLHRPVAFLVVPVFGFANAGVSFSGIG
                     PGDVMGPLPLGIALGLLLGKQIGVMLAAVLTVRTGLAPLPEGADMRSLYGVALLTGIG
                     FTMSLFIGLLAFPASETLQNAVKMGVLIGSLASGLAGYLVLRLGR"
     gene            complement(1661..2125)
                     /gene="bamE"
                     /locus_tag="PLQ11_09405"
     CDS             complement(1661..2125)
                     /gene="bamE"
                     /locus_tag="PLQ11_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HRK25155.1"
                     /translation="MSFPRTCLLALALGAPLLLTACGQDPTVRGYVLDERALEQIKPG
                     SSAESVILVLGTPSVVSTVGGKGYYYITQKLSQRYQFMQQTITDQRVLAVHLDAKNKV
                     ERVANYGLEDGKLFDFITRRTPTGGDELSFVRQLLRASNLSAPGAGPGQPGQ"
     gene            2230..2766
                     /locus_tag="PLQ11_09410"
     CDS             2230..2766
                     /locus_tag="PLQ11_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome C chaperone family protein"
                     /protein_id="HRK25156.1"
                     /translation="MILSLFRRRRKGEDIEPLYGAIMAAGLDPALYAEHGVADSFEGR
                     FEAVTLAAAIALHRLKGEPPPADAAAQQLVDRIFDGLDSAMREVGISDPGIPRRMKKF
                     AQGFYGRLGAYSQALDQTERRAALAAAIQRNLLDGRTPTPGLIDHVEALAGRIAAPGL
                     DDFLAGRWSGRAQEGQSQ"
     gene            2763..3305
                     /locus_tag="PLQ11_09415"
     CDS             2763..3305
                     /locus_tag="PLQ11_09415"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HRK25157.1"
                     /translation="MTKPDPSAWIDPLGRPVLAASIPATGLAFTVTPDAAEREALARA
                     LDLVSVDRLTADFQLRAAAGGLYEVSGMLRAEVRPRCVVTLEPFSLVIDEPVSIRFAD
                     AAAPAARRAATPAPADLDDDPPDPVENGMIDLGRVTTEFLSLALPIYPRAPGAEFAAS
                     GEDGPASPFAVLAGKGRAER"
     gene            3418..4479
                     /gene="plsX"
                     /locus_tag="PLQ11_09420"
     CDS             3418..4479
                     /gene="plsX"
                     /locus_tag="PLQ11_09420"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HRK25158.1"
                     /translation="MTRPVRIAVDAMGGDFGPSVVVPGTDLAAERHPDATFLVFGLEA
                     DVRPWLDKHPRLAGRCEFRASEVVIAMDAKPSQALRQGRGKSSMWQAIEAVKRGEADV
                     AVSAGNTGALMAMSRLCLRMMPGIDRPAIAAMWPTLRGESIVLDVGASIGADAGNLVD
                     MAIMGSAMAQIVFDLEQPTVGLLNVGVEEIKGVEQVKEAGRLLREANLPGMVYHGFIE
                     GDDIGKGTTDVVVTEGFAGNIALKTAEGTAKQIGAFMRSAMSRTLLSKIGYLLARSAF
                     DALKDRMDPRKVNGGVFLGLDGVVIKSHGGTDAIGFASAVDIAYDMVRYELLSKIRET
                     ITKSRIAPDEAAHQEEPES"
     gene            4473..5450
                     /locus_tag="PLQ11_09425"
     CDS             4473..5450
                     /locus_tag="PLQ11_09425"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HRK25159.1"
                     /translation="MVKRRAVIRGIGSYLPPRLVTNAELAGQIETSDDWIVQRTGIRA
                     RHIAGEGETTSVLATHAAERALAHAGLTAGDIDLIVLATATPDFTFPATATQVQHNLG
                     ITHGVAFDVAAVCSGFVFAITTAEKFLVSGSHKRALVIGAETFSRILDWNDRTTCVLF
                     GDGAGAVVLEAQDGTGTTADRGVLTTHLRSDGRHRAKLFVDGGPSTTGTAGHLRMEGR
                     EVFKHAVGMITDVITDAFAATGLTADDLDWFVPHQANRRIITASAEKLHIAPEKVVIT
                     VDGHGNTSAASIPLALDVAVKDGRIKPGHLVMLEAMGGGFTWGSALVRW"
     gene            5574..5891
                     /locus_tag="PLQ11_09430"
     CDS             5574..5891
                     /locus_tag="PLQ11_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HRK25160.1"
                     /translation="MMTGRTITRADLCEAVYQRLGLSRAESADLVELVLQEISDALEA
                     GEPVKLSSFGTFQVREKGERIGRNPKTGVEVPITSRRVLTFKPSNVLKARINGLDAEE
                     DDD"
     gene            6242..>6637
                     /locus_tag="PLQ11_09435"
     CDS             6242..>6637
                     /locus_tag="PLQ11_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRK25161.1"
                     /translation="MDKAPDAYRTISEAAEELDLPQHVLRFWETRFSQIKPLKRGGGR
                     RYYRPDDVELLKGIRHLLYSEGYTIKGVQRILKEQGPKAVQALMRDLIPDEDEAIETP
                     AVPPPPPFEARRESPRRIEPSLAAPAPAPV"
BASE COUNT         1057 a         2082 c         2246 g         1252 t
ORIGIN      
        1 gtgtgccatg acgcgcggag aacgacgctc ggccgcccgt ctggccgcag tacagggcct
       61 ttaccagatg gacctgaccg gcaagggcat cctcgaagtc ctcggcgaat tcgagaccca
      121 ctggatcggc aaggaaatcg agggcgatca gtacaagccg gccgaacgcg gcctgttccg
      181 cgccatcgtg cagggcgtgc tcgcccatca ggcgccgctg gatcgggcct gcgatcgcat
      241 cctggcggat ggctggccgc tgaagcgcgt cgaggcgctg atgcgcgccg ttctgcgcgc
      301 cggggcctat gagttgaccc atgccagcga tgtgccggtg aaagtggtcg tctccgaata
      361 tgtcgatatc gccgcttcct tcctcgatgg cgaggaggtc ggaatggtca atgcggtgct
      421 cgaccggctg gcgcgcgaaa cgcgggccgg ggaactggcg cggtccagct gaggagccgg
      481 gcggcaatgc gcgaacggct cggctcaacc atcaggatgt ttctggccag cgaagcggcc
      541 ggcgggctgg tgctgatggg cgcggcggcg ctggcgctga tcatcgccaa ttcaccgctg
      601 tcccacgcct atttttcgac gttgaaggcc tatctgggcg gcctgtcggt gctgcactgg
      661 atcaatgacg gcctgatggc ggttttcttc ctgctggtcg ggttggagat caagcgcgag
      721 atcatctccg gcgagctttc cacctggggc aagcgcgcat tgccggggct ggctgcgctg
      781 ggcggcgtgg tggttccggc gctgatcttc ctggccttca acccgccggg cagcgccacc
      841 agtcgcggct gggcgattcc caccgccacc gacattgcct tcgcgctggg ggttctggcg
      901 ctgctggggc cgcgcgtgcc ggcctcgctc aagatatttc tggcggcgct ggcaatcatc
      961 gatgatctgg tcgccgtgct ggtgatcgcg ctgttctata ccgacagcct gtcgttgccg
     1021 atgctggccg gggcaggggc cgtgattgcg gttctggctg tgctgaaccg gcgcggcgtg
     1081 acggcgctgt ggccttatct ggcgctcggc gccgttctct ggggtctggt gctgaaatcc
     1141 ggcgtccatg ccactattgc cggcgtggct ttggcgatga tggttccgtt gcgcaccggc
     1201 gatgaccacc ccccgttgat caggctggaa cattggctgc atcgcccggt ggcattcctg
     1261 gttgtgccgg tgttcggctt tgccaatgcc ggcgtgtcgt tttccggtat cgggcctggc
     1321 gatgtgatgg ggccgctgcc gctcggcatt gcgctggggt tgctgctcgg caagcagatc
     1381 ggcgtgatgc tggctgctgt tctgaccgtt cgcaccggcc ttgcgccgct ccccgaaggc
     1441 gccgacatgc gcagccttta cggtgtggcc ctgctgaccg gcatcggctt caccatgagc
     1501 ctgttcatcg gcctgctggc ttttccagcc agtgaaacct tgcagaacgc ggtcaagatg
     1561 ggcgtgctga tcggctcgct cgcctcgggg ctggcgggtt atctggttct gcggcttggc
     1621 cgttgagaac ccggcaggct cgcggtttga cccggcgggt ttactggccc ggctggccgg
     1681 ggcctgcgcc gggggcggac aggttggagg cgcgcagcaa ctggcgaacg aacgacagtt
     1741 catcgccgcc cgtgggcgtt ctgcgcgtga tgaaatcaaa cagcttgccg tcctcaaggc
     1801 cgtaattggc gacgcgctcg accttgttct tcgcgtccag atgcaccgcc agcacgcgct
     1861 ggtcggtaat cgtctgctgc atgaactggt agcgctgcga cagcttctgc gtgatgtaat
     1921 aatagccctt gccgccgacg gtggagacga ccgaaggcgt ccccagcacc aggatcacgc
     1981 tttcggcgct cgatccgggc ttgatctgct ccagcgcgcg ctcgtccagc acatagccgc
     2041 gaacggtcgg atcctgcccg caggcggtca acagcagggg ggcgccgagc gccagtgcca
     2101 acaggcaagt gcggggaaat gacataggcc tcttaactcc aacccgcgaa ccggtctatg
     2161 aaacatcgcc tagcccggcc atggacgatt tgcaaccacc cgaaccaaac gcgtgagttt
     2221 tcaagaccga tgatcctgtc gctgtttcgc aggcgccgca agggcgaaga tatcgagccg
     2281 ctttacggcg ccatcatggc cgccggcctc gatcctgcgc tttatgccga gcatggggtt
     2341 gccgacagtt tcgaaggccg cttcgaggcg gtgacgctgg ccgccgcgat cgccctgcat
     2401 cggctgaagg gcgagccgcc gccggccgat gccgcagccc agcaactggt cgaccggatt
     2461 tttgacgggc tcgattcggc aatgcgcgag gtcggcattt ccgatccggg cattccgcgc
     2521 cgcatgaaga aatttgcgca gggcttttac gggcggctgg gcgcctacag ccaggcgctg
     2581 gaccagaccg agcgccgcgc tgcgctcgcc gccgccatac agcgcaacct gctcgatggc
     2641 cgcacgccca cgccggggct gatcgaccat gtcgaggcgc tcgccggccg tatcgccgcg
     2701 cccggattgg atgattttct ggccgggcgc tggtccggcc gcgcgcagga aggccaatcg
     2761 caatgaccaa gccagacccc tccgcctgga tcgacccgct cggccgtccg gtgcttgccg
     2821 ccagcattcc ggcgaccggc cttgccttca ccgtgacgcc cgacgcggcc gaacgagagg
     2881 cgctggcgcg cgcgctggat ctggtttcgg tggatcggct gacggccgat tttcagctgc
     2941 gcgccgctgc cggcggcttg tatgaggttt cggggatgct gcgcgccgag gtccggccgc
     3001 gctgcgtggt gacgctggag cctttttctc ttgtgatcga cgagccggtg tcgatccgtt
     3061 ttgccgatgc cgccgctccc gccgcgcgcc gcgcggcaac gccagccccg gccgacctcg
     3121 atgatgaccc gcccgatccg gtggaaaacg gcatgatcga tcttggccgc gtcaccaccg
     3181 agttcctgtc gctcgcgctg ccgatctatc cgcgcgcgcc gggggccgag tttgcggcct
     3241 cgggcgagga tgggccggcc tcgccctttg ccgttctggc aggcaagggc agggcggagc
     3301 ggtgatcggc gaccatccac aggcttgccc tcacccgcac atatgcttat gtgcgcagcg
     3361 caagagttgc gggcccacct tccggtgcgg tttcaggaag gcgtgtttgg cgatcccatg
     3421 accagaccag ttcggattgc agtggatgcc atgggtggcg attttggccc ctccgtggtt
     3481 gtgcctggca ccgatctggc ggccgaacgg catccggacg ccactttcct ggtttttggc
     3541 ctcgaagcgg atgtgcgtcc ctggctggac aagcacccgc ggctggccgg acgctgcgaa
     3601 ttccgtgcct ccgaggtcgt catcgccatg gatgccaagc cgagccaggc tctgcgtcag
     3661 gggcgtggca agtcgtcgat gtggcaggcc atcgaggcgg tcaagcgtgg cgaggccgat
     3721 gtggcggttt cggccggcaa caccggcgcg ctgatggcga tgtcacggct ctgcctaaga
     3781 atgatgcccg gtatcgaccg gccggcgatt gccgcgatgt ggccgacgct gcgtggcgag
     3841 tcgatcgtgc tcgatgtcgg cgcctcgatc ggtgccgacg ccggcaatct cgtcgacatg
     3901 gcgatcatgg gctcggcgat ggcgcagatc gttttcgatc tggaacagcc gacggtgggg
     3961 ttgctgaatg tcggggtcga ggaaatcaag ggcgtggagc aggtcaagga ggccggccgc
     4021 ctgctgcgcg aggccaatct gccgggcatg gtctatcacg gtttcatcga gggcgatgac
     4081 atcggcaagg gcaccacgga tgtcgtggtc accgaagggt tcgccggcaa tatcgcgctg
     4141 aaaaccgccg aaggcaccgc caagcagatc ggcgccttca tgcgttcggc gatgagccgc
     4201 accctgttgt cgaagatcgg ctacctgctg gcgcgcagcg ccttcgatgc cctgaaggac
     4261 cggatggacc cgcgcaaggt caatggcggc gtctttctcg gtctcgatgg tgttgtcatc
     4321 aagagccatg gcggcaccga tgcgatcggt tttgcctcgg ccgtggatat cgcttacgac
     4381 atggtgcgtt acgaactgct gtcgaagatc cgtgaaacga tcaccaagtc gcggatcgct
     4441 cccgacgagg cggcgcatca ggaggagcct gaatcgtgaa acgccgtgcc gtcatccggg
     4501 ggatcgggtc ctatctgccg ccgcgtctgg tcaccaacgc cgaactggcg gggcagatcg
     4561 aaacctctga cgactggatc gtccagcgca ccggcatccg ggcgcgccat atcgccgggg
     4621 agggcgagac gacctccgtg ctggccaccc atgcggccga gcgtgccctg gcccatgccg
     4681 ggctgacggc cggagacatc gatcttatcg tgctggcgac ggccacgccc gatttcacct
     4741 tcccggccac ggcgacccag gtgcagcaca atctggggat cacccacggt gttgcattcg
     4801 atgtcgcggc ggtctgttcc ggctttgtct ttgccatcac gacggcggag aaattccttg
     4861 tctcgggatc gcacaagcgg gcgctggtga tcggtgcgga gacattctcg cgcattcttg
     4921 actggaacga ccggacgacc tgcgtgctgt tcggcgatgg cgcgggcgcc gtggttctcg
     4981 aggcgcagga tggcaccggc accaccgccg atcggggcgt cctgaccacg catcttcgct
     5041 ctgacggacg tcaccgggcc aagctgtttg tcgatggcgg gccgtcgacc accggcaccg
     5101 ccggccattt gcgcatggaa ggccgcgaag tgttcaaaca tgccgtcggc atgatcaccg
     5161 atgtgatcac cgatgccttc gccgccactg gcctgactgc cgatgatctc gactggttcg
     5221 tgccgcatca ggccaatcgc cgcatcatca ccgccagcgc cgagaagctg catatcgcgc
     5281 cggaaaaggt ggtgatcacc gtcgatggtc atggcaacac ctcggcggcg tcgatcccgc
     5341 tcgcgctcga tgtggcggtc aaggatggcc gcataaagcc cggccacctt gtcatgcttg
     5401 aagcaatggg cggcgggttc acctgggggt cggcgctggt gcgctggtaa ttggcaccgt
     5461 ccgggcatcg agattcgtcg cgcaatttgt cgcgcagacg ttgacccatg ctctaaacct
     5521 ttctaaaact ttatctttgt gattagggtt ggccacaagg ccttcgggag agggtgatga
     5581 ccggtagaac gattacgcgg gcggacctgt gcgaggcggt gtatcagcgc ctcggcttgt
     5641 cgcgggccga atcggcggat ctggtcgaac tggtgctaca ggaaatctcc gatgcactgg
     5701 aggcgggtga gccggtcaag ctgtcctcgt tcggcacctt tcaggtgcgc gagaagggcg
     5761 agcggatcgg ccgcaacccc aagaccgggg tcgaggtgcc gatcacctca cggcgggtgc
     5821 tgacgttcaa gccgtccaat gtgctcaagg cacggatcaa cggcctcgat gccgaagagg
     5881 acgacgattg ataccggcgc gccgtgcagg gcgacgacag cggattattc caacggtcat
     5941 cgggtttgac cgatggccgt tggttgggag cgaatgcgac gccgagccca tgcgaggaat
     6001 ttcggtccga aactgacccg atctgccgaa ggcggatcgg tcggtcctga ggaccgctgg
     6061 tattatttgc agcctggccc cgccgcatcg cgaaaaaagt ttgctggcac acactggtcg
     6121 cggcgcgaac aggctaacct tggcatgaga caattgattc gccgcgcctg ccgctgacgg
     6181 tcttgccgtc cctggctcca tgcgctgcgc gacagccgtg aaatgccggg gaggcaccgt
     6241 gatcgacaag gcgcccgatg cctatcgcac catcagcgag gcagccgaag aactcgacct
     6301 gccgcagcat gtgctgcgct tctgggagac ccggttttcg cagatcaagc cgctcaagcg
     6361 tggtggcggc cggcgctact atcgccccga tgatgtggaa ttgctcaagg gcatccgcca
     6421 tctgctctac agcgagggat acacgatcaa gggcgtgcaa cgcatcctga aggagcaggg
     6481 gcccaaggcg gttcaggcgc tgatgcgcga cctgattccg gatgaggacg aagcgattga
     6541 aaccccggcg gtgcccccgc cgccgccgtt tgaggctcgt cgcgagtccc ccaggcgcat
     6601 cgagcccagc cttgcagctc cggcgcccgc gcctgtc
//
LOCUS       DAOMXG010000136         6631 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_6099_length_6631_cov_11.537865, whole genome shotgun sequence.
ACCESSION   DAOMXG010000136 DAOMXG010000000
VERSION     DAOMXG010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 6631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6631
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6099_length_6631_cov_11.537865"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(505..1284)
                     /locus_tag="PLQ11_09440"
     CDS             complement(505..1284)
                     /locus_tag="PLQ11_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018426053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRK25162.1"
                     /translation="MSALPAIEVRNLNVHFGTAHVLRDVSFSVAAGESFGLVGESGSG
                     KSTVLRALSGLNPNWSGTVRFNGRDHVTRPARDFYRQVQMVFQDPFGSLHPRLTVDAC
                     LAEPLAIHGIGDPDRRIEQALADVGLDHGFRFRYPNQLSGGQRQRVAIARALILEPSI
                     LLLDEPTSALDVSIQAEVLNLLQDLKNRRDLTMVLVSHNLAVVGHMCSRVAVMNGGVI
                     VEEADIDTLRRQGGRHPYTQELLAASRHVALAQPMDQPAMV"
     gene            complement(1286..2131)
                     /locus_tag="PLQ11_09445"
     CDS             complement(1286..2131)
                     /locus_tag="PLQ11_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRK25163.1"
                     /translation="MTDETPLIEVDNLRVRFHTPTRIVEAVRGLSFKLGREKLAIVGE
                     SGSGKSMTGRAILDLVPWPGEVLADRLAFRGEDLRTMDRTRRRALRGKSISMVMQDPK
                     FSLNPVMSVGKQISEAFRLHHRLTGAEARDRTLAMLEAVQIREPGRVYDLYPHEVSGG
                     MGQRIMIAMMLVGEPDVLIADEPTSALDVTVQAQVLQIMERLVSERGMGLILISHDLP
                     LVSAFCDRVIVMYAGRAMETLDAKALGKGRHPYTKGLIECLPDLDYPKERLAVLNRDP
                     AWIGP"
     gene            complement(2144..3031)
                     /locus_tag="PLQ11_09450"
     CDS             complement(2144..3031)
                     /locus_tag="PLQ11_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRK25164.1"
                     /translation="MSLYTYLTSERPASRRQAQLGQYYRQWQALKQNPLALMGLVIVI
                     ALLMLAALAPWVAPHDPIAQNLDQRLLAPSAKHFFGTDALGRDIFSRIVYGTRITLVI
                     VLLVVISVGPLGLLIGTLSGYFGGIIDTILMRVTDIFLAFPRLVLALAFVAVLGPGIE
                     NAVIAIACTAWPPYARVARAETMVIRNADYIAAVRLQGASNARIVLKHVVPICIPSVI
                     VRTTLDMAGIILTAAGLGFLGLGAQPPLPEWGAMIAAGREFVFDQWWVATFPGLAICL
                     VALGFNLLGDGLRDVLDAK"
     gene            complement(3037..4068)
                     /locus_tag="PLQ11_09455"
     CDS             complement(3037..4068)
                     /locus_tag="PLQ11_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018426056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRK25165.1"
                     /translation="MAAMTGPGRVLLAVSRELVTIVVTILGLLAVTFLIARVMPVDPV
                     LAVVGDNARPDVYEKARLEMGLDKPLLVQFWLYLNKVAAGDFGRSVLTSKLVLDDIKR
                     VFPATLELATLGTLIGISLGIPLGVLAATRQGRWPDHIARFIGLFGYSIPIFWLGLMG
                     LLLFYAKLGWVAGPGRIDVAYQDFVTPMTGVLLIDCALQGEWDAFRNAARHLILPSAV
                     LGLFSTAYISRMTRSFMINELQQHYVTTARVKGLSERRVVWGHALRNAMVPLLTVIAL
                     SYANLLEGSVLTETVFAWPGLGRYITNSLLNADMNAVLGGTVVVGLIFVGINLLTDVL
                     ARAVDPRAR"
     gene            complement(4141..5754)
                     /locus_tag="PLQ11_09460"
     CDS             complement(4141..5754)
                     /locus_tag="PLQ11_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003499025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRK25166.1"
                     /translation="MIPLSFNRRGALLAAAILSSTALTGVAPALAQTPKDTIVMARQI
                     DDIISLDPGEAFEFSGVEVVTNVYDKLVGVDIAKNNALVGDLATSWTVSDDSLTYTFK
                     LRPGVKFHSGNPLTAADVVYSFQRAVTLNKSPGFILAQFGLTKENVLEKVKAVDDLTV
                     QIVVNKPYAPTFFLNCMTSGVASIVDSKTVKANEKDGDFGNGWLKANSAGTGAYSLRA
                     WRAGEQVALEANPNWFKGAPKNKRVIIRHVAEPASQRLLLEKGDVDYARNLHKDALTA
                     IKSNPNLQTVQGTKGYILYLGLNTKNPNFAKPEVREAMKWLVDYEAIERNIVQGTFQQ
                     HQSFLPQGFLGAITDKPYKFDVAKAKELLAKAGLPNGFSVTMDVRSIAPIADIAQAIQ
                     ASWAQAGVKLELIPGDGKATLTKYRARQHDIYIGQWGPDYLDPHTNAETFAINVDNTD
                     DAKSKTLAWRNAWDIPEMTKTTQANVLERDADKRKAVYEALQREHQKSSPFVIMYQQI
                     EVAAYGKQVSGFVVGPTSDTNYFHTIEKK"
     gene            complement(5900..5974)
                     /locus_tag="PLQ11_09465"
     tRNA            complement(5900..5974)
                     /locus_tag="PLQ11_09465"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5939..5941),aa:Glu,seq:ttc)
     gene            complement(6074..>6631)
                     /locus_tag="PLQ11_09470"
     CDS             complement(6074..>6631)
                     /locus_tag="PLQ11_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HRK25167.1"
                     /translation="GSGRSIPGVDLGRAVRQAVEAGVLVKGGGHAMAAGITVLRDRIA
                     AFQTFVEDRLAADVARARADRALKIDAALTPASATPALLKDIEAAGPFGQGNPEPVFA
                     FPAVRLVDLFEVGQGHLKLRLVGGDGARLGAVAFRAAQSPLGQALVRAKGSTLHVCGS
                     LALDRWGGRESVELRLSDAAPAGRR"
BASE COUNT         1239 a         2101 c         2124 g         1167 t
ORIGIN      
        1 tcggtggcct tcttgtcggt ggccttcttg tccgtggcct gcttgtcggt ggccttcttg
       61 tcagtcgcgc ccttgtcggt cgcctgcttg tccttggaca tgatgatttc ctccaatggt
      121 cgtttttggg tggggcgcca gtcgccctga gcccgagatc aagctagcga aggcatttcg
      181 atccggcaat gaatatccga ccgatcggtc agggtatttc tctacgccgc ctgtgaccga
      241 ttacgacggc cgtttccata aaaatacatt gaaaaacaat aatttgaatc tctagcagca
      301 aagcagggca gggccttgtc tgcccgaacg gccggaaagg ctggagatcg acgcttgagg
      361 cagagtgtcg cagatagagc atgtcgcgat taagtggata ccgtttaatc gcagaagaca
      421 tgctctaact tattgatacc gatcacgtta tccgcatttt gatgattcca tcaaaatgcg
      481 gcgtgatcta gcgtgaccgt ccggtcagac cattgccggc tgatccatcg gctgggccag
      541 ggccacatgg cgcgaggcgg cgagcagttc ctgcgtatag ggatggcggc cgccctgacg
      601 gcgcagcgtg tcgatgtccg cttcctcgac gatcacgccg ccgttcatca cggcgacgcg
      661 gctgcacata tggccgacca cggcgaggtt gtggctgacc agcaccatgg tcaggtcgcg
      721 ccggttcttc aagtcctgca acaggttgag cacttcggcc tggatcgaca catcgagggc
      781 cgaggtcggc tcgtcgagca gcaggatcga cggttcgagg atcagcgcgc gggcgatggc
      841 gacgcgctgg cgctgcccgc cggagagctg gttcggatag cgaaagcgga acccgtgatc
      901 gaggccgaca tctgccagcg cctgttcgat gcgacggtcc ggatcgccaa tgccgtggat
      961 ggccagcggt tcggccaggc aggcatcgac cgtcaggcgc ggatgcagtg atccgaacgg
     1021 atcctgaaac accatctgga cctgacgata gaagtctctg gccggtctcg tcacatggtc
     1081 gcggccgttg aaccggaccg tgcccgacca gttcggattg aggccggaga gcgcgcgcag
     1141 cactgtcgat ttgcctgagc ccgattcccc caccagtccg aagctctcgc cggcggcgac
     1201 agagaagctg acatcgcgga gcacatgggc cgtgccgaaa tggacgttga ggttgcggac
     1261 ctcgatggcg ggcagggcgc tcatgtcagg gtccgatcca ggcagggtcg cgattgagca
     1321 cggccagccg ctctttcggg taatcgagat cgggcaggca ttcgatcagc cccttggtgt
     1381 agggatgccg gcccttgccg agggccttgg cgtcgagcgt ttccatcgcc cggccggcat
     1441 acatgacgat cacgcggtcg cagaaggccg agaccagcgg cagatcgtga ctgatcagga
     1501 tcagccccat gccgcgctcg gagacgaggc gctccatgat ctggagaacc tgcgcctgca
     1561 ccgtcacatc gagcgccgag gtcggctcgt ccgcgatcag cacatccggt tcacccacca
     1621 gcatcatggc gatcatgatg cgctgcccca tgccgccgga gacctcgtgc ggataaagat
     1681 cgtaaacccg gcccggctcg cggatctgca ccgcctcaag catcgccagc gtccggtcgc
     1741 gcgcttcggc gccggtcagc cggtgatgca gccggaaggc ttcgctgatc tgcttgccga
     1801 cgctcatcac cggattgagc gagaatttcg gatcctgcat caccatcgag atcgacttgc
     1861 cgcgcaaggc ccgccgtcgc gtcctgtcca ttgtcctgag gtcctcgccg cggaaggcga
     1921 gacggtcggc cagcacctcg ccgggccaag gcaccagatc aaggatggca cggccggtca
     1981 tcgacttgcc ggagcccgat tccccgacga tcgccagctt ctcgcggcca agcttgaagg
     2041 acaggccgcg caccgcctcg acaatgcgcg tcggggtgtg gaaccgcacc ctgagattgt
     2101 cgacctcaat gagcggggtc tcgtccgtca tgccaagtcc ccctcacttt gcatccagaa
     2161 catcgcgcag cccgtcgccc agcaggttga agccaagtgc caccaggcag atcgccagac
     2221 cggggaaggt cgccacccac cattgatcga acacgaattc gcgccccgca gcgatcatcg
     2281 ccccccattc gggaaggggt ggctgggcgc cgaggccaag aaatccgagt ccggcagcgg
     2341 tcaggatgat cccggccatg tcgagcgtcg tgcgcacgat caccgaggga atgcagatcg
     2401 gcaccacatg cttgagcacg atccgcgcgt tggaggcgcc ctgcaaccgg acggcggcga
     2461 tgtaatcggc gttgcggatt accatcgttt cggcgcgcgc cacccgcgca tagggtggcc
     2521 aggcggtgca ggcgatggcg atcaccgcat tctcgattcc cggccccagc acggcaacga
     2581 acgccagcgc cagcaccagg cgcggaaagg cgagaaagat gtcggtcacc cgcatcagga
     2641 tcgtatcgat gatgccgccg aaatagcccg acagtgtgcc gatcagcagc cccagcggcc
     2701 cgaccgagat caccaccagc agcacgatga ccagggtgat ccgcgtgccg tagacaatac
     2761 ggctgaagat gtcgcggccg agcgcatcgg tgccgaagaa atgcttggcc gagggagcaa
     2821 gcagccgctg atcgagattc tgcgcgatcg gatcgtgagg cgcgacccag ggcgccagcg
     2881 ccgccagcat cagcagcgcg atcacgatca ccagccccat cagcgccagc ggattctgct
     2941 tcagtgcctg ccactggcgg taatactggc cgagttgggc ctgccgtcgc gaggcgggac
     3001 gttccgaggt gagataggtg taaaggctca tcgcgctcac cgtgccctgg gatcgacggc
     3061 gcgggccagc acatccgtca gcaggttgat gcccacgaag atcagcccga ccaccacggt
     3121 gccgccaagc actgcgttca tgtcggcatt gagcagggaa ttggtgatgt agcggccaag
     3181 accgggccag gcgaacaccg tctcggtcag caccgagcct tccagcaggt tggcatagga
     3241 cagcgcgatg acggtgagca gcggcaccat cgcgttgcgc agggcatggc cccagaccac
     3301 ccgccgctcg ctcaatccct tgacccgtgc cgtggtcacg taatgctgct gcaactcgtt
     3361 gatcatgaac gagcgcgtca tccggctgat ataggcggtg gagaacaggc cgagcaccgc
     3421 cgagggcagg atgaggtgac gcgctgcgtt gcggaatgcg tcccattcgc cctgcagcgc
     3481 gcaatcgatg agcagcacgc cggtcatcgg cgtgacgaaa tcctgatacg caacatcgat
     3541 ccgccccggc ccggccaccc agccgagttt ggcgtagaac agtagcaggc ccatcagccc
     3601 cagccagaaa atcgggatcg aatagccgaa aagcccgatg aaccgggcga tatggtcggg
     3661 ccagcggccc tgccgggttg ccgccagcac gcccagcgga atgccgaggc tgatgccgat
     3721 cagcgtgccc agcgtcgcca gttcgagcgt tgccggaaac acccgcttga tatcgtcgag
     3781 caccagtttg gaggtcagca ccgaccggcc gaaatcgccg gcggcgacct tgttcagata
     3841 gagccagaac tgcaccagca gcggcttgtc caggcccatc tccagccgtg ctttttcata
     3901 gacatcgggc cgggcattgt cgccgaccac cgccagcacc ggatcgaccg gcatcacccg
     3961 ggcgatcagg aaggtgacgg ccagcaggcc gaggatcgtc acgacgatcg tcaccagctc
     4021 ccgcgaaacc gccagcagga cacgacccgg acccgtcatt gccgccatga tactcaaccc
     4081 ccaaacgacc gcaccggccg caatcggtca gattgcggcc ggcccaatgg cgttgcagcc
     4141 ttacttcttc tcgatcgtgt ggaagtagtt ggtgtccgag gtcgggccga cgacgaagcc
     4201 ggaaacctgc ttgccgtagg ccgccacctc gatctgctga tacatgataa cgaagggcga
     4261 ggacttctgg tgctcgcgct gcagcgcctc atagaccgcc ttgcgcttgt cggcatcgcg
     4321 ttcgagcacg ttggcctggg tggtcttggt catctccggg atgtcccagg cattgcgcca
     4381 ggccagcgtc ttggactttg catcgtcggt attgtcgaca ttgatggcga aggtctcggc
     4441 gttggtgtgc ggatcaagat aatccggccc ccactggccg atgtagatgt catgctggcg
     4501 ggcgcgatat ttggtcagcg tcgccttgcc gtcgccgggg atcagttcga gcttgacgcc
     4561 ggcctgggcc cagcttgcct ggatcgcctg ggcgatatcg gcaatcggcg cgatcgagcg
     4621 cacatccatg gtcaccgaaa agccgttcgg caggcccgcc ttggcgagca gttccttggc
     4681 cttggcgaca tcgaacttgt agggcttgtc ggtgatcgcg ccgaggaagc cctgcggcag
     4741 gaagctctgg tgctgctgga acgtgccctg aacgatgttg cgctcgatcg cttcgtaatc
     4801 gaccagccac ttcatcgcct cgcgcacttc cggtttggcg aaattgggat ttttggtgtt
     4861 gaggccgaga tagaggatat agcccttggt gccctggacc gtctgcaggt tcgggttgga
     4921 cttgatggcg gtcagcgcat ccttgtgcag gttgcgtgca taatcgacat cgcccttttc
     4981 gagcagcagg cgctgcgatg cgggttcggc cacatggcgg atgatgacgc gcttgttctt
     5041 cggtgcgccc ttgaaccagt tcggattggc ctcaagcgcc acctgttcgc cggcccgcca
     5101 ggcgcgcagg ctgtaggcgc cggtgccggc cgaattggcc ttgagccagc cattgccgaa
     5161 gtcgccgtcc ttttcgttgg ccttgaccgt cttggaatcg acgatcgagg cgacgcccga
     5221 ggtcatgcag ttgagaaaga atgtcggcgc atagggcttg ttgacgacga tctgcacggt
     5281 gagatcatca accgccttga ccttctccag cacgttttcc ttggtcaggc cgaactgggc
     5341 gagaatgaag ccgggcgact tgttcagcgt caccgcccgc tggaaggaat agaccacgtc
     5401 ggccgcagtc agcgggttgc cggaatggaa cttgacgccc ggccgcagct tgaacgtgta
     5461 ggtcaggctg tcgtcgctca ccgtccagct ggtggcaaga tccccgacca gcgcattgtt
     5521 cttggcgata tcgaccccga ccagcttgtc atagacattg gtcacaacct cgacgccgga
     5581 aaattcgaag gcctcgcccg gatcgagcga gatgatgtcg tcgatctggc gcgccatcac
     5641 gatcgtgtcc ttcggggtct gcgccagcgc gggcgcaacg ccggtcaggg ccgtcgagga
     5701 caggatcgcg gcagccaaca acgcgccgcg gcgattgaac gaaaggggga tcatgctggg
     5761 cctccagaaa ttgcccgact tgagagcgag cgacttcccg catcagagac ccggctccga
     5821 ctaaagtcaa tcacagtcct gtggcctttg cgccagacct gcaaggctcg catcgtctct
     5881 ggcagagggg gggaagcaat ggcgcgccct acgggagtcg aacccgtctt gccagattga
     5941 aaatctggag tcctaaccga tagacgaagg gcgcatcgca gcggcgcgag gcgccgtgca
     6001 gcggggttcg tataaaggtc cggatcgttc gcggcaaggg ctttcagcgg ctttttgaca
     6061 accgggcagg ggcctatcgc cggccggccg gcgcggcatc gctgagccgg agttcgacgc
     6121 tctcgcgtcc gccccagcga tcgagcgcca gcgagccgca gacgtgcagg gtgcttccct
     6181 tcgcccgcac cagcgcctgg ccgagcggcg attgcgctgc acggaacgcc accgcgccaa
     6241 gccgcgcgcc atccccgccc accagtcgca gtttgagatg gccttggccg acttcgaaca
     6301 gatcgacgag gcgcacggca gggaaggcga ataccggctc cggattgccc tgtccgaacg
     6361 gtccggccgc ctctatgtcc ttcagcagcg ccggggttgc cgaggcgggc gtcagcgccg
     6421 cgtcgatctt cagcgcgcgg tcggcgcggg cacgggcgac atcggcggcg aggcgatcct
     6481 caacgaatgt ctggaacgcg gcgatcctgt cgcgcaggac cgtgattccc gctgccatgg
     6541 catggccgcc gcccttgacg agaacgccgg cctccaccgc ctgccgcacc gcgcggccga
     6601 ggtcgacgcc ggggatcgag cggcccgaac c
//
LOCUS       DAOMXG010000137         6567 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_6176_length_6567_cov_10.186271, whole genome shotgun sequence.
ACCESSION   DAOMXG010000137 DAOMXG010000000
VERSION     DAOMXG010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 6567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6567
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6176_length_6567_cov_10.186271"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1317)
                     /locus_tag="PLQ11_09475"
     CDS             complement(<1..1317)
                     /locus_tag="PLQ11_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HRK25168.1"
                     /translation="MVTRVATVAFEGIEARPVDVQVQVASGSVAFILVGLPDKAVAES
                     KERVRAALTASGLAMPAKRVVVNLAPADLPKEGSHFDLPIALGIMAAIGAVPSDALNG
                     FTVLGELALDGAISAVAGVLPAAIAANARGHGLICPRASGPEAAWAGGEMDVLAPASL
                     IQLANHFKGTQVLSRPEPVISAAASTLPDLKDVKGQETAKRCLEIAAAGGHNLLMCGP
                     PGAGKSMLAQRLPSILPPLTPRELLEVSMVHSIAGLLTDGALTNRRPFRSPHHSASIA
                     ALTGGGLHARPGEVSLSHHGVLFLDELPEFQPQALDSMRQPLETGEVSVSRANNRVTY
                     PARFSLVAAMNPCKCGRALEPGYACKRAPNDKCMAQYQARLSGPLIDRFDLYVEVAAV
                     TAADLVLPPPSEGSAEVAVRVAAARQRQAARYEALGLDGRTNAAAPA"
     gene            1687..2658
                     /locus_tag="PLQ11_09480"
     CDS             1687..2658
                     /locus_tag="PLQ11_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042918 - alkanesulfonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HRK25169.1"
                     /translation="MIFNRRTFLATAASVAASSLARPALAQKLGKITVDWATYNPLSL
                     IIRDNKWLEDEFKADGTAIQWVQSAGSNKALEFLNAGSIDFGSTAGAAALIGKIGGNP
                     IKSVSVYSRPEWTALVTKKDEIKSVADLKGKSVAVTRGTDPHIFLIRALLDHKVAASD
                     VKFVLLQHADGKTALIRGDVDAWAGLDPIMAQAEVEDGARLFYRKPENNTWGVLNVRE
                     DFAAKNPEVVKRVIAVYERARRHALANPAELKAALVKAARLPDNVIERQLTRTDLSTS
                     VIGPVQAEAIEQAGVALQQAEVIKADVNVKATVAALIDPSYTAGLKS"
     gene            2680..3519
                     /locus_tag="PLQ11_09485"
     CDS             2680..3519
                     /locus_tag="PLQ11_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRK25170.1"
                     /translation="MSDAEAVQSRSQTPTDARRGSPRSALFGNLGVGLLLPLTLALGW
                     ELAVSAGLSNGRLVPPPSRIWLTLVDLWKTGDLLKHILATLGRVGAGFALGALAGTIL
                     GAISGAVPIFRRLTDPLFQGLRSVPSIAWVPLFILWFGIFEAPKVTLIAVGVFFPVYL
                     GVMGAIQSVDRKLIEVGRMFRLGRTEMMRRILLPAILPSWAIALRSGLGLGFMFVVAA
                     EFMGASEGLGYLLVDGQQLGKPDQIVAAIIVFAMLGKLWDGILVAVTAPLLAWQDTAR
                     AEL"
     gene            3521..4306
                     /locus_tag="PLQ11_09490"
     CDS             3521..4306
                     /locus_tag="PLQ11_09490"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019906385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRK25171.1"
                     /translation="MLTLERLSKTYVGGNVALKDVSLTLKPGEIVAMVGGSGCGKTTL
                     LRLVAGLDAPTQGSVAVDEAIITAPNEAIGLIFQEPRLLPWLTVADNIAFGLSGLAKD
                     EQKARVDFALHRVGLDGYNDRWPRQLSGGQAQRVAISRALAPEPKVLLLDEPFSALDA
                     FTRASLHEHLLALWEAKRPTLLIVTHDVEEAVALADRVIVMQPHPGRIFAEIAIDSDR
                     PRDPLSDEFLALKRRILKALDGSLAEARAATAKTDQPAAAAWW"
     gene            4326..4841
                     /locus_tag="PLQ11_09495"
     CDS             4326..4841
                     /locus_tag="PLQ11_09495"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009338356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRK25172.1"
                     /translation="MDADALRALQAPLKARYKEDGAAAILTLKASGQIDESKIACKVE
                     TGRAIAEAGLHPFTGGSGAELCSGDMLLEALVACAGVTLKAVSTALGIDLKAGRVSAE
                     GDLDFRGTLGVAKEAPVGFQAIRLAFDLDTDAGEEQIAQLIKLTERYCVVFQTLKQSP
                     ASTVTVRTGKA"
     gene            complement(4953..>6567)
                     /gene="mutS"
                     /locus_tag="PLQ11_09500"
     CDS             complement(4953..>6567)
                     /gene="mutS"
                     /locus_tag="PLQ11_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRK25173.1"
                     /translation="DLRRILRTAPDLPRCLTRLAINRGGPRDLAALAQGIRAAIAIAA
                     LLPPEAPTEIAGAAERLRHVDTRLADELERALADELPLLRRDGGFVRAGYDPALEEAW
                     ALKDDSRAVIAGLQQRYAADTDCRSLRIKYNNMLGYFIEVPQVAGERLVQPPLNALFV
                     HRQTMAGAMRFSTTELSELEARIASAGERAIGIEIRIFDDLASRVLAVDAALRGAAEA
                     MAIIDVTAALADLAADRGWTRPDIAEDGAFHIEAGRHPVVEAALARGGASFVANDCEL
                     HGAGEAGEILLVTGPNMAGKSTYLRQNALIAILAQMGSFVPARLARIGIVDRLFSRVG
                     AADDLARGRSTFMVEMVETAAILNQATARSLVILDEIGRGTATYDGLSIAWATIEHLH
                     EKTRCRALFATHFHELTVLSKRLNRLRPVTMRVTEYRGDVVFLHEVVPGAADRSYGIQ
                     VAKLAGLPAPVVERARAVLKELEKTDRAAPVARLVDDLPLFALTRPEPVAPRPDPLRE
                     AVAALNPDEMSPREALDALYRLKAIKPEA"
BASE COUNT         1149 a         2175 c         2139 g         1104 t
ORIGIN      
        1 ggcgggcgcg gcggcgttgg tgcggccgtc aagccccaac gcctcataac gggcggcctg
       61 acgctgccgt gcagccgcca cgcgcaccgc cacctccgcc gatccttccg agggcggcgg
      121 cagaacgaga tcagcggcgg tgacagccgc aacctcgaca taaagatcaa accggtcgat
      181 cagcggcccg gaaagccgcg cctgatactg cgccatgcat ttgtcattgg gcgcgcgctt
      241 gcaggcgtag cccggctcga gggcacggcc gcatttgcac ggattcatcg ccgcaaccag
      301 agagaaacgg gccggatagg taacccggtt gttggcgcgg gataccgaga cctcgccggt
      361 ttccagcggc tggcgcatcg aatcgagcgc ctgaggctgg aattccggca attcatcgag
      421 aaacaggaca ccgtgatggc tgagcgacac ttctcctgga cgtgcgtgca gcccgccgcc
      481 ggtgagagcc gcgatcgaag ccgaatgatg cggcgagcgg aacgggcgcc gattggtcag
      541 cgcgccatcg gtgagcaatc ccgcgatgga atggaccatc gacacttcga gcagttcacg
      601 cggcgtcagc ggtggcagga tcgagggcag gcgctgggcc agcatcgact tgccggcccc
      661 gggcggtcca cacatgagca ggttgtggcc gcccgccgcc gcgatctcca ggcaccgctt
      721 ggcggtctcc tgtcccttga catcctttag atccggaagc gtactcgctg cggcgctgat
      781 caccggctct gggcgggaga gcacctgggt tcccttgaaa tggttggcca actggatgag
      841 ggaggcagga gccagcacat ccatttcgcc accggcccag gcggcctccg gccccgacgc
      901 acgcggacag atcaaaccat ggccacgcgc attggccgca atggccgccg gcaggacccc
      961 tgctaccgcc gaaattgcgc cgtcaagcgc cagttccccg aggaccgtaa agccattcag
     1021 ggcatcggaa ggaacagcgc cgatggctgc catgatgccc agggcgatgg gtaaatcgaa
     1081 gtggctgccc tccttgggca gatcggccgg ggcaagattg accaccacgc gtttggccgg
     1141 catggccagt ccggaagccg tgagcgcggc gcgcacccgc tctttcgatt cggccacggc
     1201 cttgtcagga agccctacca gaatgaaggc gaccgaaccg gacgctacct gcacctgcac
     1261 atcgacgggc cgggcctcga tgccctcgaa tgcgaccgtt gctacgcgcg tgaccataat
     1321 ccactctacc tcgtgaccga tgttagccag cttgcggcaa cgggcaagcc ttcatgctgg
     1381 ccgatcattg cgtcagatcc ccgctcagga ccggctccgg cgctcgacga ctgcacgcgc
     1441 agtgcgcttg tcgacagccg gtcggcatgc tcgcacctgt ccccggcggc tcccttcgag
     1501 cgcggggcag cgctcgcatg cagcgcaaag cctgcaacgc ttgaggtacc gcccatggca
     1561 ttccgttctt aaaaaagaac attttgtttg gtttgggttg acgagaccaa ttcgttcgat
     1621 taaaagaacg acagcctcct ccaaaggccc gaacgtcaga cgaccatcca gtcccggaac
     1681 acgaccatga tcttcaatcg ccgcaccttc ctcgccaccg ccgccagcgt cgccgcctcg
     1741 tcgctggcac ggcccgccct cgcgcagaag ctcggcaaga tcacggtcga ctgggccacc
     1801 tacaaccccc tcagcctgat catccgcgac aacaagtggc tcgaagacga gttcaaggcc
     1861 gacggcaccg ccatccagtg ggtgcagtcc gccggctcga acaaggcgct ggaattcctc
     1921 aatgccggct ccatcgactt cggttccacc gctggtgcag cggccctgat cggcaagatc
     1981 ggcggcaacc cgatcaagtc cgtatcggtc tattcgcgcc ccgaatggac cgcacttgtc
     2041 accaagaagg acgagatcaa gtccgttgcc gatctcaagg gcaagtcggt cgccgtgacg
     2101 cgcggcaccg acccccacat tttcctcatc cgcgcgctgc tcgaccacaa ggttgcggcg
     2161 agcgatgtga aattcgtgct gctgcagcac gccgatggca agaccgcgct catccgcggc
     2221 gatgtcgacg cctgggccgg gcttgacccg atcatggcgc aggccgaggt cgaggatggc
     2281 gcgcggctgt tctaccgcaa gccggagaac aacacctggg gcgtgctgaa cgtgcgcgag
     2341 gatttcgcag ccaagaaccc tgaagtggtc aagcgcgtta tcgctgtcta cgagcgggct
     2401 cgcagacatg cgctggccaa tccggcggaa ctgaaggcgg cgctggtcaa ggccgcgcgt
     2461 ctgccggaca acgtcatcga gcggcagctc acccgcacgg acctctccac ctcggtgatc
     2521 ggaccggtgc aggccgaggc catcgaacag gccggcgtcg cgctgcaaca ggccgaggtg
     2581 atcaaggccg atgtcaacgt caaggcaacg gttgcagcct tgatcgatcc gtcctacacg
     2641 gcagggctga aatcctgatc caagtgaggc gcccgcggca tgagtgacgc cgaggctgtg
     2701 cagagtcgtt cgcaaacccc gacggatgcc cgtcggggtt cgcctcgttc agctctgttt
     2761 ggcaacctcg gcgtcggtct gctgctgccc ctgacgctgg cgcttgggtg ggagctggcc
     2821 gtgagcgccg gcctgtccaa tggccggctg gtgccgccgc caagccgcat ctggctcacg
     2881 ctggtcgacc tgtggaagac gggcgatctt ctcaagcaca ttctggcgac gcttggccgt
     2941 gtcggcgccg gttttgcgct gggcgcactg gcagggacga tcctcggcgc catttccggc
     3001 gcagtgccga ttttccgcag gttgaccgac ccgctgttcc agggcctcag atcggtgccg
     3061 tcgatcgcct gggtgccgct gttcatcctg tggttcggca ttttcgaagc acccaaggtg
     3121 actctgatcg cagtgggcgt gttcttcccg gtctatcttg gcgtgatggg cgccatccag
     3181 tcggtcgatc gcaagctgat cgaggttggg cggatgttcc ggcttggccg gaccgagatg
     3241 atgcggcgca tcctgctgcc ggcgatcctg ccctcctggg cgattgcgct gcgttccggg
     3301 ctcgggctcg gattcatgtt cgtggtggcg gccgagttca tgggcgcatc cgaagggctc
     3361 ggatatctgc tggtcgatgg tcagcagctc ggcaagccgg accagatcgt cgccgcgatc
     3421 atcgtctttg cgatgctcgg caaattatgg gacggcattc tggtggctgt gacagcgccg
     3481 ctgctggcct ggcaggatac ggcgcgggcg gagctgtgaa atgctgacgc tcgaacgact
     3541 gtcgaaaacc tacgttggcg gcaatgtcgc gctgaaggac gtttcgctga ccctgaagcc
     3601 gggcgagatc gtggccatgg tcggtggctc cggctgcgga aaaaccacgc tgctgcggct
     3661 tgtcgccggg cttgatgcgc caacccaagg gtcggtcgcg gtggacgagg ccatcatcac
     3721 agcgccgaac gaggcgatcg gcctgatctt tcaggaaccg cgcctgctgc cgtggctgac
     3781 ggtggccgac aacatcgcct tcggcctgtc cggcctggca aaagacgagc agaaggcgcg
     3841 cgttgacttt gcgctccacc gtgttggcct tgatggctac aatgaccgct ggccccgcca
     3901 gctttccggc gggcaggcac agcgtgtcgc catttcgcgg gcgctggcgc cggagccgaa
     3961 agtgctgctg ctggatgagc cgttctcggc ccttgatgcc ttcacccgcg cctccctgca
     4021 cgaacatctg ctggctttgt gggaggccaa gcggccgacg ttgctgatcg tcacccacga
     4081 tgtcgaggaa gcggtggcgc tggccgaccg ggtcatcgtc atgcaaccgc atccgggccg
     4141 catcttcgcc gagatcgcca ttgacagcga ccggccgcgt gacccgctct cggacgagtt
     4201 tctggcgctc aagcgccgca tcctgaaggc gctcgacggc tcgctggccg aggcgcgcgc
     4261 cgcaaccgcc aagaccgatc aacccgctgc tgccgcctgg tggtaggaca atcaaggatc
     4321 ctgcaatgga cgccgacgcc cttcgtgccc ttcaagcacc gctcaaggcc cgctacaagg
     4381 aggatggcgc cgccgccatt ctgaccctga aggcaagcgg gcagatcgac gaatccaaga
     4441 tcgcctgcaa ggtcgagacc gggcgcgcca ttgccgaagc ggggctgcat ccgttcaccg
     4501 gcgggtcggg cgccgaactc tgctcgggcg acatgctgct cgaagcgctg gtcgcctgcg
     4561 ccggggtgac gctgaaggcg gtctcgaccg cgctgggaat tgacctgaag gccggcaggg
     4621 tgagtgccga aggcgatctc gatttccgcg gcacgctcgg cgttgccaag gaggcgccgg
     4681 tcggctttca ggcgatccgg ctcgcgttcg atctcgacac cgatgccggc gaggagcaga
     4741 tcgcccagtt gatcaagctc accgagcgct attgcgtcgt tttccagacg ctgaagcaga
     4801 gcccggcctc gacggtcacg gtccggaccg gcaaggcgtg aagggcttgt agtcgtggcc
     4861 cgcacgttgg accatttggt cccgtgcgga ccgaccctcg caacgccgcc ctgggcggct
     4921 gctcggcatc gctttcgctc ctatcctcga cattacgcct ccggcttgat ggctttgagt
     4981 cgatagagcg cgtccagcgc ctcgcgcggg gacatctcgt cgggattgag cgcagcgaca
     5041 gcctcgcgca aaggatcggg gcgcggagcg accggctccg gccgggtgag agcgaacagc
     5101 ggcaggtcat cgaccagtcg ggcgaccggc gccgcccggt cggtcttttc aagctctttc
     5161 agcaccgccc gcgcccgctc cacgaccggc gccggcaaac cggcaagctt ggccacctga
     5221 atgccatagg agcgatcagc cgcgcccggc accacctcat gcagaaacac cacatcgccg
     5281 cgatattcgg ttacccgcat ggtcaccggg cgcaggcggt tcagccgctt ggacagcacc
     5341 gtcagttcgt ggaaatgggt cgcgaacagc gcccggcagc gggtcttttc gtgcaggtgc
     5401 tcaatcgtcg cccaggcgat ggagaggccg tcataggtgg ccgtgccgcg gccgatctca
     5461 tcgagaatga ccagactgcg ggcggtggcc tgattgagaa tggcggcggt ctcgaccatc
     5521 tcgaccatga aggtggaacg gccgcgcgcc agatcatcgg cggcgccgac ccggctgaac
     5581 agacggtcaa cgataccaat gcgggcaaga cgggcgggca cgaaggatcc catctgtgcc
     5641 agaatggcaa tcagtgcatt ctgcctgaga taggtcgatt tgccggccat gttcgggccg
     5701 gtgaccagca atatctcgcc agcctcgccg gccccatgca attcgcaatc gttggcgaca
     5761 aagctcgcgc cgccccgcgc cagcgccgcc tcgaccaccg gatgccgccc cgcctcgata
     5821 tggaaggcac catcctcggc gatgtccggc cgtgtccagc cgcgatcggc cgcaagatcg
     5881 gccagcgcgg cggtgacatc gatgatcgcc atcgcctcgg ccgctccgcg caaggcggca
     5941 tcgacggcca gaacgcggga agcaagatca tcgaagatcc tgatctcgat gccgatggcc
     6001 cgctcgccgg ccgaggcgat gcgcgcctcc agctccgaca attcggtggt tgaaaaccgc
     6061 atggcacccg ccatggtctg gcgatgcacg aacagggcat tgagcggcgg ctgcaccagt
     6121 cgctcgccgg cgacctgcgg cacctcgatg aaataaccga gcatgttgtt gtatttgatg
     6181 cgcaggctgc ggcagtcggt gtccgccgca tagcgctgct gcaggccggc gatcaccgcg
     6241 cggctgtcat ccttcagcgc ccacgcctcc tccagcgccg gatcataccc ggcgcgcaca
     6301 aagccgccgt cacgcctgag caacggcagt tcatcggcca gtgcgcgttc gagttcgtcg
     6361 gccagcctcg tatcgacgtg cctgagccgt tcggctgccc cggcaatctc ggtcggggcc
     6421 tccggcggca acagcgcggc aatggcaatg gccgcgcgaa tgccttgcgc cagcgcggca
     6481 aggtcccgcg ggccgccccg gttaatagcc aggcgtgtga ggcagcgcgg caggtcgggt
     6541 gcggtgcgca agatccggcg caggtca
//
LOCUS       DAOMXG010000138         6548 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_6201_length_6548_cov_9.033883, whole genome shotgun sequence.
ACCESSION   DAOMXG010000138 DAOMXG010000000
VERSION     DAOMXG010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 6548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6548
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6201_length_6548_cov_9.033883"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="PLQ11_09505"
     CDS             <1..919
                     /locus_tag="PLQ11_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRK25174.1"
                     /translation="AAVTAALTGLVRAGDHIVSSRALFGSCRYVVEEHLPRFGVESTL
                     VDGRDLAAWRAAVRPNTRAFFLESPSNPTLEIIDIAAVAKIAHAAGAKLVVDNVFATA
                     LWQQPLKLGADCVVYSATKHIDGQGRCLGGVILASQAFIDEHVHTLLRQTGPAMSPFN
                     AWVLLKSLETFPLRLRRQSETAVVVADWLAGHPRIGQLRYPGRKDHPDAALIARQMQA
                     GSTLMCFEVGDSKASAYAFLNRLNIIRISNNLGDAKSLAVHPATTTHQRFTPAQRQEM
                     GVLDSLVRLSIGLEHPDDLIADLRQALEA"
     gene            991..1785
                     /locus_tag="PLQ11_09510"
     CDS             991..1785
                     /locus_tag="PLQ11_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRK25175.1"
                     /translation="MTSQARASAKANPRVALFATCLVDLMRPNVGFAAVKLLEQAGCT
                     VEVPVQTCCGQPAFNNGDRKTAAALAAQMAEAFAPYDYVVVPSGSCGGMIRTHYPELF
                     AGDPDLGPRIEAVAAKTYELVSFLTDIMGVTEVAARYEGTVTYHDSCSGLRELGIRQQ
                     PRKLLASVEGLTLAEMNETESCCGFGGTFAVKYGELSDAIVSKKAANIEASAATTVLA
                     GDLGCLMNMAGKLQRRGSKVRARHIAEVLAGMADGPAIGEPAGKDG"
     gene            1789..1938
                     /locus_tag="PLQ11_09515"
     CDS             1789..1938
                     /locus_tag="PLQ11_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25176.1"
                     /translation="MAEVSASPALGRIGWLALAFLAVLAAALWWRFGSAVFTELMAAA
                     WSLCF"
     gene            complement(1954..2574)
                     /locus_tag="PLQ11_09520"
     CDS             complement(1954..2574)
                     /locus_tag="PLQ11_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRK25177.1"
                     /translation="MARLRAADYDDKRRALLDVAARLFATAGFDRTSISEIAGAAGVS
                     KALLYHYYSGKDEVLFDIIHSHIQELVQQVEQADRRDLPPVDHLRGLIVATLEAYRDA
                     DTMHKVQINELSKLSDERQQVIRALERRLVTIMAGAILRINPALADVAESRLLKPVTM
                     ALFGVLNWTYLWFRDGKPLTREAYGDIVTKLFVDGVMRMVEADAKT"
     gene            complement(2614..3924)
                     /gene="paaF"
                     /locus_tag="PLQ11_09525"
     CDS             complement(2614..3924)
                     /gene="paaF"
                     /locus_tag="PLQ11_09525"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016557512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HRK25178.1"
                     /translation="MLDLTPNRKDLDPIEIASRDEIAALQFHRLKRTLRHAYENVAHY
                     RATFDAAGATPDDLKSLADLAKFPFTTKKDLRDTYPFGMVAVPMDQVARVHASSGTTG
                     KPTVVAYTKADLDVWANVVARSIRASGGRPGMKVHVAYGYGLFTGGLGAHYGAEKLGC
                     TVIPMSGGMTERQVQLITDFKPDIIMVTPSYMLAILDEFRRQGIDPRTSSLKVGIFGA
                     EPWTNAMRGEIEDAFDMHAVDIYGLSEVMGPGVANECVETKDGLHVWEDHFYPEIIDP
                     VTGAVLPDGEVGELVFTSLTKEALPIIRYRTRDLTRLLPGTARSMRRIEKITGRSDDM
                     IILRGVNVFPTQIEEQILKCTGLTPHFFIELSRKGRLDTMSVVVEARPGATDAEARKA
                     SAKLLAHHIKAVVGISTDIRVVDPGGVERSGGKARRVFDLRPKL"
     gene            complement(3954..4376)
                     /gene="paaI"
                     /locus_tag="PLQ11_09530"
     CDS             complement(3954..4376)
                     /gene="paaI"
                     /locus_tag="PLQ11_09530"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008194868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HRK25179.1"
                     /translation="MNADDIAKKSTELMWAEDNASAHLGMKLVHVGPGKAVLTMPVAV
                     SMTNGHGTCHGGYIFTLADSAFAFACNSYNQRCVAAHCSITYLAPARAGMLLRATAAE
                     QQRVDRNGIYDITITDDATGAVIAEFRGHSRTIAGTFF"
     gene            complement(4366..5187)
                     /gene="paaG"
                     /locus_tag="PLQ11_09535"
     CDS             complement(4366..5187)
                     /gene="paaG"
                     /locus_tag="PLQ11_09535"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014329389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HRK25180.1"
                     /translation="MSAGSSEPELLISRADGVLTLTLNRPHRFNAFTVSLHEALIDAL
                     RQAAADPGIRSLLLTGAGRGFCAGQDLSDRAVAPGSARPDLGLSLDKRYNPLIRCIRA
                     MGKPVICAVNGVAAGAGANVALACDIVLAARSARFLQAFAKLGLVPDSGGSWLLGRLI
                     GDARARAAILLAEPISAEQAADWGMIWKVVPDDELMTEATGLARRLADGPTYGYGLIK
                     QALDAAATRSLSEQLDLERDLQRKAGASDDYAEGVRAFTEKREPRFTGRGPDDER"
     gene            5325..>6548
                     /gene="paaN"
                     /locus_tag="PLQ11_09540"
     CDS             5325..>6548
                     /gene="paaN"
                     /locus_tag="PLQ11_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008550029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaN"
                     /protein_id="HRK25181.1"
                     /translation="MSEFASRHAEVLERALKAISERTYWSAYPEMPSGKIYGETAKAD
                     AAAAFAALKGQPFRFDASHPADRYVGAETSPFDGKGLGITYPAAGVERLVAASGAAGS
                     GWAAASVEDRAGVLIEALHRVNRQSFLVAEAVMHTSGQAFAMAFQAGGPHAQDRALEA
                     IAYAVAEMRRVPQQVTWEKPQGKGDPLRLQKRFRIVPRGVAVVIGCATFPTWNTYPGL
                     FASLATGNTVIVKPHPAAILPVALSLKILREVLSEAGYDPNVVLLAADTADAPITKEL
                     VTAEAVRIVDFTGGPAFGGWLRANAGGRQVYTEEAGVNGIVIDSTASFRAMCDNIAFS
                     LALYSGQMCTAPQNIFVPASGIASDEGTKSFDEVASGIAAAVDRLLGDPVRAAAVLGA
                     VQSEVTLARIARAAAT"
BASE COUNT         1107 a         2057 c         2137 g         1247 t
ORIGIN      
        1 ggcggcggtc accgcggcgc tgaccgggct tgtccgggcc ggcgatcaca tcgtctcctc
       61 gcgggcgttg tttggctcct gccgttatgt ggtcgaggag cacctgccgc ggttcggcgt
      121 tgaaagcacg ctggtcgatg gtcgcgatct tgctgcctgg cgcgcggccg tgcggcccaa
      181 cacgcgcgcg ttctttcttg aaagcccgtc caatccgacg ctcgaaatca ttgatatcgc
      241 cgcggtggcc aagattgccc atgcggcggg cgccaagctg gtcgttgaca atgtattcgc
      301 cacggctctc tggcagcagc cgctgaagct tggcgccgat tgcgttgtct attcggcgac
      361 caagcatatc gatgggcagg gccggtgcct cggcggcgtg atccttgcct cccaggcctt
      421 tattgacgaa catgtccaca ccctgctgcg ccagaccggg ccggcgatgt cgcccttcaa
      481 tgcctgggtc ctgttgaagt cgcttgagac tttcccgctg cgcctgcgcc gtcagtccga
      541 aacggcggtg gttgttgctg actggctggc gggccatccg aggatcggtc aattgcgcta
      601 tcccggacgc aaggatcatc ccgatgctgc gctgattgcc cggcagatgc aggcgggttc
      661 aacactgatg tgtttcgagg tcggtgacag caaggcttct gcctatgcgt ttctcaatcg
      721 gctgaacatc atccgcattt ccaacaatct gggcgatgcc aagagccttg ccgtgcatcc
      781 ggcgacgacc acgcaccagc gcttcacccc ggcccagcgg caggaaatgg gtgttttgga
      841 ttcgctcgtg cgcttgtcga tcgggcttga acaccccgac gacctgattg ccgacctcag
      901 gcaggcgctt gaggcttgaa gcgcgccggc aattgcgcca ctctggcgcc gcccagccgg
      961 catgggccgg agcgacaaga ggccagcgac gtgacatccc aagccagagc ttccgccaaa
     1021 gccaatccgc gcgtagcgct gtttgcgacc tgtcttgtcg acctgatgcg gccgaatgtc
     1081 ggttttgctg ccgtcaagct gctggagcag gccggttgca cggtcgaggt tccggtgcag
     1141 acctgctgcg gccagccggc tttcaacaac ggcgaccgca agacggcggc cgcccttgcg
     1201 gcacagatgg ccgaggcctt cgctccatat gattatgtcg tcgtgccgtc cggctcctgc
     1261 ggtggcatga tccgcaccca ttatcccgag ctgttcgccg gcgatccgga cctgggcccg
     1321 cgtatcgagg ctgtggcggc gaaaacctat gagctggtct cgtttctgac cgacatcatg
     1381 ggtgtgacgg aggtcgccgc ccggtacgag ggaaccgtca cctatcacga cagctgctcc
     1441 ggtctgcgcg aactcggcat tcgtcagcag ccgcgcaagc tgcttgcaag cgttgaggga
     1501 ctgacccttg ccgagatgaa cgaaaccgag agctgctgcg gtttcggcgg cacctttgcg
     1561 gtcaagtatg gcgaattgtc cgatgctatc gtcagcaaga aggccgccaa tatcgaggcg
     1621 agtgcggcca cgacggtgct tgccggggat ctcggctgcc tgatgaacat ggccggcaag
     1681 ctgcaacggc ggggctcgaa ggtgcgtgcc cgccatatcg ccgaggttct ggccggaatg
     1741 gcggatggcc cggcaatcgg cgagccggca ggcaaggacg gctgagcgat ggcggaggta
     1801 tcggcaagcc cggcgctcgg caggatcggc tggctggcct tggctttcct ggccgttctg
     1861 gcagccgcgc tgtggtggcg tttcggctct gccgtcttta ccgaattgat ggctgctgcc
     1921 tggagcctgt gtttctgatc cacgcgagga ttgctacgtc ttggcgtcgg cctcgaccat
     1981 ccgcatcaca ccatcgacga acagcttcgt gacgatgtcc ccataggcct cgcgcgtcag
     2041 cggcttgccg tcacggaacc ataggtaggt ccagttcagc acgccgaaca gcgccatggt
     2101 caccggtttg agcaggcgac tctcggcgac gtcggccagc gccgggttga tacgcaggat
     2161 ggcgccggcc atgatggtca ccagccgccg ctcgagtgcc cgaatcacct gctggcgttc
     2221 gtcggacagt ttggacagtt cgttgatctg caccttgtgc atggtgtcag cgtcacgata
     2281 cgcctcgagc gttgcgacaa tcaggccgcg cagatggtcc accggtggca ggtcgcggcg
     2341 atcagcctgc tcaacttgct gcaccagctc ctggatgtgg ctatggatga tgtcgaacag
     2401 aacctcatcc ttgcccgaat aatagtgata gagcagcgcc ttcgacacgc cagccgcgcc
     2461 ggcgatctcg ctgatcgagg tccggtcgaa tcctgcagtg gcaaaaagcc gtgcggccac
     2521 gtcaagcagg gcacggcgct tgtcatcgta gtctgcggcg cggaggcggg ccaagggaaa
     2581 accctttcga caggttaggc ctccgcgccg gtatcacagt tttggccgga ggtcgaacac
     2641 cctgcgtgcc tttccaccgg aacgctcgac cccgccggga tcaaccaccc ggatgtctgt
     2701 ggaaatgccg acgacggcct tgatgtgatg ggccagcaac ttggcggatg ccttgcgggc
     2761 ctccgcatcc gtggcgccgg ggcgcgcttc caccaccacg ctcatcgtgt ccagccggcc
     2821 cttgcggctg agttcgatga agaaatgcgg cgtcagcccc gtgcatttga ggatctgctc
     2881 ctcgatctgg gtggggaaca cgttgacccc gcgcaggatg atcatgtcat ccgagcggcc
     2941 ggtgattttc tcgatacggc gcatcgaacg ggctgtgccg ggcaacagcc gcgtcaggtc
     3001 gcgcgtccgg tagcggatga tcggcagcgc ttccttggtc agcgaggtga acaccaattc
     3061 gcccacttct ccgtccggca agacggctcc ggtcaccgga tcgatgatct cgggatagaa
     3121 atggtcctcc cagacatgca ggccatcctt ggtctcgaca cattcattgg caacgccggg
     3181 gcccatcact tcggacaggc cgtagatgtc aaccgcgtgc atatcgaacg catcctcgat
     3241 ctcgccacgc atcgcattgg tccacggttc ggcgccgaaa atcccgacct tgaggcttga
     3301 ggtgcgcgga tcgattccct ggcggcggaa ctcgtcgagg atcgccagca tgtaggaggg
     3361 cgtcaccatg atgatgtccg gcttgaagtc ggtgatcagc tgcacctgcc gttcggtcat
     3421 gccgcccgac atggggatga cggtgcagcc cagtttctcc gctccgtaat gcgcgccaag
     3481 gccgccggtg aacagcccat agccataagc gacatgcacc ttcatgccgg gccgtccgcc
     3541 cgatgcccga atggagcgcg ccaccacatt ggcccagaca tcaagatcgg ccttggtata
     3601 ggccacgacc gtcggcttgc cggtggtccc ggacgaggcg tgcacgcgcg cgacctgatc
     3661 catcggcacg gcgaccatgc cgaacgggta ggtgtcacgc aggtccttct tcgtggtgaa
     3721 ggggaatttg gcgagatcgg caaggctctt gaggtcgtcc ggcgttgcac ccgctgcatc
     3781 gaaggttgcc cgataatgcg ccacattctc ataggcatgg cgcaacgtcc gcttcagccg
     3841 atggaactgg agggccgcta tttcgtcgcg cgaggcaatt tcgatcggat cgaggtcttt
     3901 gcggttcggt gtcaggtcga gcatggcagg ttcccggaaa ttggcgcgca gacttagaag
     3961 aatgtgccag cgatggtgcg gctgtgacca cgaaattcgg cgatcacggc gccggtcgca
     4021 tcatcggtga tggtgatgtc gtagatgccg ttgcgatcga cccgctgctg ttcggcggct
     4081 gtggcgcgca acagcatgcc ggcccgcgcc ggcgccagat aggtgatcga gcaatgggcg
     4141 gcgacacagc gctggttgta gctgttgcag gcaaaggcaa aggcggaatc agccagcgtg
     4201 aagatatagc cgccgtggca ggtgccgtga ccgttggtca ttgagaccgc gaccggcatg
     4261 gtcagcaccg ctttgcccgg cccgacatgg accagcttca tgcccagatg cgccgaggcg
     4321 ttgtcctcgg cccacatcag ttcggtgctc ttcttggcaa tatcgtcagc gttcatcgtc
     4381 ggggcctcgt ccggtaaagc ggggttcgcg tttttcggtg aaggcgcgca cgccttcggc
     4441 atagtcatcg gaggcgccgg ccttgcgttg caggtcgcgt tcaagatcga gttgctcgga
     4501 cagcgagcgg gtcgctgccg catccagtgc ctgtttgatc agcccgtatc cataggtggg
     4561 gccgtccgcg aggcgtctgg caagccccgt cgcctcggtc atcagctcat cgtccgggac
     4621 aaccttccag atcattcccc aatcggcggc ctgttcggcg ctgatcggct ctgccagcag
     4681 aatcgccgcc cgcgcccggg catcgccgat cagccgtccg agcagccagc tgccgcccga
     4741 atcaggcacc agccccagtt tggcaaaggc ctggaggaag cgggccgagc gggcggccag
     4801 aacaatgtcg caggcaagtg caacattggc gccggccccc gccgccacgc cattgaccgc
     4861 gcagatgacc ggcttgccca tcgcacggat gcagcggatc aaaggattgt agcgcttgtc
     4921 cagcgacaag ccgagatcgg gccgcgcgct gcccggcgca acggcgcgat ccgacagatc
     4981 ctgcccggcg cagaagccgc gccccgcgcc ggtcagcagc aaggagcgga tgccgggatc
     5041 ggcagccgcc tggcgcaagg cgtcgatcag ggcctcgtgc agcgaaacgg tgaaggcgtt
     5101 gaagcgatgc gggcggttga gcgtcagcgt cagaacgccg tccgcgcggc tgatcagcaa
     5161 ttccggctct gatgatccgg ccgacatggg ccctccctgc gcatcctttt atccccatcc
     5221 ggccggatga ggaaatcgtt acgggcatct ttacattgac cgaatgcttg gtcaataatg
     5281 cttcaaacag aaatcggcgc ggtctgcccc gccgggagga tgtcatgagt gaatttgcca
     5341 gccgtcacgc ggaagttctg gagcgcgccc tcaaggcgat atccgagcgg acctactgga
     5401 gcgcctatcc ggaaatgccc tccggcaaga tctatggcga gaccgccaag gccgatgcgg
     5461 ctgctgcctt tgccgctttg aaggggcagc cgttccggtt cgatgcaagc catccggcag
     5521 atcgctatgt cggcgcggag acgtctcctt tcgacggcaa ggggctgggc attacctatc
     5581 cggctgccgg cgtcgaacgg cttgtggccg cttcgggtgc tgcgggctcc ggctgggccg
     5641 ccgcgtccgt cgaggatcgg gccggggtcc tcatcgaggc gctgcaccgg gtgaaccgcc
     5701 agagcttcct ggtggctgag gcggtgatgc atacctcggg ccaggctttc gccatggcct
     5761 tccaggctgg cggtccgcat gcgcaggatc gcgcgctgga ggccattgcc tatgcggttg
     5821 ccgaaatgcg ccgcgtgccg cagcaggtga cctgggaaaa gccccagggc aagggcgatc
     5881 ccttgcgcct gcagaagcgc ttccgcatcg ttccgcgcgg tgttgcggtt gtgatcggct
     5941 gcgcgacctt cccgacctgg aacacctatc ccggcctgtt tgccagcctt gcgaccggca
     6001 ataccgtcat cgtcaagccg caccctgcgg cgatcctgcc ggtggcattg tcgttgaaga
     6061 tcctgcgcga ggtgctgtcc gaggccggat atgatcccaa tgtcgtgttg ctcgcggccg
     6121 atacggccga tgcgccgatc accaaagagc tggtgacggc tgaagccgtg cgcattgtcg
     6181 atttcaccgg tgggccggcc ttcggcggct ggctgcgcgc caatgctggt ggcaggcagg
     6241 tctataccga ggaggccggg gtcaacggta tcgtgatcga ttccaccgcc agcttccgcg
     6301 ccatgtgcga caacatcgcg ttctcgctgg cgctttattc cggccagatg tgcaccgcac
     6361 cgcagaatat cttcgtcccc gccagcggca ttgccagcga cgaagggacg aagtcgtttg
     6421 acgaggtcgc atcgggtatc gcagccgccg tggaccggct actgggcgat ccggtgcggg
     6481 cggcggccgt tctgggcgcg gtgcagagcg aggtcacgct ggcgcgcatc gcccgtgcgg
     6541 ctgcgacc
//
LOCUS       DAOMXG010000139         6511 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_6250_length_6511_cov_8.720105, whole genome shotgun sequence.
ACCESSION   DAOMXG010000139 DAOMXG010000000
VERSION     DAOMXG010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 6511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6511
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6250_length_6511_cov_8.720105"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    61..160
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(279..998)
                     /locus_tag="PLQ11_09545"
     CDS             complement(279..998)
                     /locus_tag="PLQ11_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease activity regulator RraA"
                     /protein_id="HRK25182.1"
                     /translation="MPLSPATRDQLKKVSVATLCSVLFKRGLRNQVIQGARPLNPGLP
                     VMVGEAYTVRYMPAREDRNPMSVFQNREHPQRKAVEECPPGHVLVFDSRKDHRAASAG
                     GILVSRLMVRGVAGVVTDGGFRDSHEIAALAIPAYHVQPSAPTNLTLHEAIAINDPIG
                     CGDAPVFPGDVIVGDQEAVIVIPAGIADAVADEATAMTVYEDFVIDMVQNHGRPILGL
                     YPATDTQSLIDFEAWRKQHNR"
     gene            complement(1059..2798)
                     /gene="araD"
                     /locus_tag="PLQ11_09550"
     CDS             complement(1059..2798)
                     /gene="araD"
                     /locus_tag="PLQ11_09550"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinonate dehydratase"
                     /protein_id="HRK25183.1"
                     /translation="MAKARKKPEELRSARWFAPDDLRSFGHRSRMNQMGYDVAEFAGK
                     PIIGIINTWSDFAQCHAHFRARVEDVKRGVLMAGGFPVELPAISVSESTVKPTSMLYR
                     NFLAMETEELIRSQPVDGVVLMGGCDKTTPGLILGATSADVPAIFMPAGPMLRGNFQG
                     KQLGSGSDVWKYWDERRAGNITEQEWYDMQTGIARSYGVCMTMGTAATMTGIADVLGL
                     CLPGASAIPAADSGHIRMASACGRRAVEMVWEDLKPADIMTRQAFLNAIVVAMAMGCS
                     TNAVIHLIAMARRAGHDIGLAEFDATSRKVPVIANIRPSGDKYLMEDFYYAGGMLGLM
                     SRLTDHLDLSQINATGKTWKTSLKGAKVFNDDVIRTPDNAIYKEGALAVLTGNIAPNG
                     CVIKPSAAGETFQKHSGPALVFDSYEEMKAVIDRDDLDVTENTVLILRNAGPLGGPGM
                     PEWGMLPIPKKLVKQGVRDMVRLSDARMSGTSYGACILHVSPESYIGGPLALVKTGDI
                     IDLDIPARSLNMRVSDAELDRRRKAWRKPAPRYERGFGWMFSQHISQAEKGCDFDFLE
                     TDFGAPVGEPAIN"
     gene            complement(2877..3620)
                     /locus_tag="PLQ11_09555"
     CDS             complement(2877..3620)
                     /locus_tag="PLQ11_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HRK25184.1"
                     /translation="MSEAGKGGGKGGGSRDLAVRLKNARAHKHSSQQWLQRQLNDPYV
                     AQAKRDGYRSRAAYKLIEIDDRHHLLKPGQRIVDLGAAPGGWSQVAARRVDSINGRGK
                     VVGIDLLDIEPIAGVDFAVMDFNDADAPERLKAMLGGAADGVLSDMAANTTGHKKTDH
                     LRIIGLAELAAEYAREVLSPGGYFLCKLFQGGETAALVTALKRDFAVVRHIKPAASRA
                     DSAELYVLATGFRGHAGDGPAGVPAGTGG"
     gene            complement(3617..4591)
                     /locus_tag="PLQ11_09560"
     CDS             complement(3617..4591)
                     /locus_tag="PLQ11_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HRK25185.1"
                     /translation="MYAALDLGTNNCRLLVARPSRNGFRVVDAFSRIVRLGEGFGSEK
                     RLRPAAMQRTLDALKICRDKIAFRGVTRARFITTEACRAAVNGEEFLASVRQQTGLAL
                     EILDGREEAHLAAAGCAVLADRAAEDVIIFDIGGGSTEMVWLRGRQGHNQPTRQRIAD
                     WASLTVGVVTLAERHGGINVTPESFEAMVADASEHLAGFARSARRATEGANFHLLGTS
                     GTVTTLAGLHLGLERYDRRQVDGLWMSDRQVETVLQTLLGMTYDQRAANGCIGAERAD
                     LVLPGCAIFEAIRRAFPSKRVRIADRGLREGILVELMTADGLWGEGGR"
     gene            4859..5236
                     /locus_tag="PLQ11_09565"
     CDS             4859..5236
                     /locus_tag="PLQ11_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006940050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25186.1"
                     /translation="MSNANLKRIIVHLARTKAFPNGSPNHGYEFVAPLDASNHIDADA
                     WHKLRDKCRVTRFWGGEEEDIGHLVRKPGGSWAFHYDVKGEEDDEAGYRFAAHAFVER
                     EYVSIKDQDGELQPFVVASVEDA"
     gene            5509..>6511
                     /gene="tyrS"
                     /locus_tag="PLQ11_09570"
     CDS             5509..>6511
                     /gene="tyrS"
                     /locus_tag="PLQ11_09570"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRK25187.1"
                     /translation="MSAYSSDFLRVLNERGFIHQCSDFAGLDARAARGEVVAYVGYDC
                     TARSLHIGNFLSLMMLYWLQQSGNKPITLMGGGTTRIGDPSGKDETRALRSIEEIDAN
                     KAAIKGVFAKILTFGAGRTDAIMLDNAEWLPELKWIDMLRDVGRHFSVNRMLSMDSVK
                     LRLDREHEMSFIEFNYMIAQSYDFVELNRRTGCNLQMGGSDQWGNIIMGVDLGRRMGT
                     EQLYALTTPLLTTASGAKMGKTARGAVWLNAEDFSPYDFWQYWRNTEDADVGRFLKLF
                     TTLPMEEIARLEALGGAEINEAKKVLASEATALLHGRAEADKAAETARLAFEQGVAAA
                     "
BASE COUNT         1162 a         2060 c         2021 g         1168 t
ORIGIN      
        1 acctatggag accgtgaatc acatctcaat ggaaattaat aggtcctgag cctagtcttg
       61 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tcttttcgca ttttcttgac
      181 gcgaaccgga agccacttcg cttgaaaatg ctctagtctt gtcttttcgc attttcttga
      241 cgcgaaccgg aagccacttc gcttgaaaat gctctaattc agcggttgtg ctgcttgcgc
      301 caggcctcga agtcgatcag cgactgggtg tcggttgcgg gatagaggcc gaggatcggg
      361 cggccgtggt tctgcaccat gtcgatgacg aaatcctcat agacggtcat ggcggtggcc
      421 tcgtcggcga ccgcgtcggc gatccccgcc ggaatgacga tcaccgcttc ctgatcaccg
      481 acgatgacat cgcccggaaa caccggagca tcgccgcagc cgatcggatc gttgatggcg
      541 atcgcctcgt gcagggtcag gttggtcggc gccgagggct ggacgtgata ggcggggatc
      601 gccagcgccg cgatctcgtg gctgtcgcgg aagccaccat cggtgacgac gccggcaacg
      661 ccgcgcacca tcagccggct gacgagaatg ccgccggccg aggcggcgcg atggtccttg
      721 cggctgtcga acaccagcac atggcccggc gggcattcct ccaccgcctt gcgctgcgga
      781 tgctcccggt tctggaacac gctcatcggg ttgcgatcct cgcgcgccgg catgtagcgc
      841 acggtatagg cctcgcccac catcaccggc aggccgggat tgagcggccg ggcgccctgg
      901 atcacctgat ttctgagacc gcgcttgaac agcaccgagc acagcgtggc gacggagacc
      961 ttcttcagct ggtcgcgggt ggcgggggaa aggggcatcg ggacaatcct caaacgctgc
     1021 tgtccctccc ctgatgggga gagacaaact caagtggatc aattgattgc cggttcaccg
     1081 accggcgcgc cgaaatcggt ctcaaggaaa tcgaagtcac agcccttctc ggcctgggaa
     1141 atatgctgcg agaacatcca gccgaagccg cgttcatagc gcggcgcggg cttcctccat
     1201 gccttgcggc gtcgatcgag ttcggcgtcg ctcacccgca tgttgaggct gcgggcgggg
     1261 atgtcgagat cgatgatgtc gccggttttc accagcgcca gcggcccgcc gatgtagctt
     1321 tcagggctga catgcaggat gcaggcgcca taggaggtgc cggacatgcg cgcatcggaa
     1381 agccgaacca tgtcgcgcac gccctgtttc accagctttt tcgggatcgg cagcatgccc
     1441 cattccggca tgccggggcc gccgagcggg ccggcattgc gcaggatcag cacagtgttt
     1501 tccgtcacgt cgagatcgtc ccgatcaatc accgccttca tctcctcgta gctgtcgaac
     1561 accagcgccg ggccggaatg cttctggaat gtctctccgg ctgcactcgg cttgatgacg
     1621 cagccattgg gggcgatgtt gccggtgagc accgccagcg cgccctcctt gtagatggca
     1681 ttgtccggcg tgcggatcac gtcatcattg aacacttttg cgcccttgag gcttgtcttc
     1741 caggtcttgc cggtagcgtt gatctgggaa aggtcaagat gatcggtcag ccggctcatc
     1801 aggccgagca tgccgccggc atagtagaaa tcctccatca ggtatttgtc gcccgagggg
     1861 cggatgttgg cgatcaccgg caccttgcgc gaggtggcat cgaattcggc aaggccgatg
     1921 tcgtgcccgg cgcggcgggc catcgcgatc agatgaatca cggcattggt ggaacagccc
     1981 atcgccatgg caacaacgat ggcattgagg aacgcctgcc gggtcatgat gtcggcgggt
     2041 ttgagatcct cccacaccat ctcgaccgcg cgacggccgc aggcggaagc catgcggata
     2101 tggccggaat cagccgccgg aatggccgag gcgccgggca ggcacaggcc gagcacatcg
     2161 gcgatgccgg tcatcgtcgc cgccgtgccc atggtcatgc acacgccgta ggagcgggcg
     2221 atgccggtct gcatgtcata ccattcctgc tcggtgatgt tgccggcgcg acgctcatcc
     2281 cagtatttcc agacatcgga accggaaccc aattgcttgc cctggaaatt gccgcgcagc
     2341 atcggccccg ccggcatgaa gatcgccggc acgtccgccg aggtcgcgcc gaggatgagg
     2401 ccgggcgtgg tcttgtcgca gccgcccatc agcaccacgc catcgaccgg ctgcgagcgg
     2461 atcagctcct cggtctccat ggcgaggaaa ttgcgataga gcatcgaggt cggcttgacc
     2521 gtcgattccg acaccgagat cgccggcagt tccaccggaa aaccgccggc catcagcacg
     2581 ccgcgcttaa catcctcgac gcgcgccctg aaatgggcgt ggcactgggc gaaatcggac
     2641 caggtgttga tgatgccgat gatcggcttg ccggcgaatt cggcgacatc atagcccatc
     2701 tgattcatgc ggctgcgatg gccaaacgag cgcaggtcat cgggagcgaa ccagcgggcg
     2761 gaacgcagct cttccggctt tttcctagcc ttggccatag gggacgggtc ctcgatcgag
     2821 caggtttgca gttatcatac aaccgcccgg ccggctgtcc atgcacctgg agaggatcag
     2881 ccgccggtcc ctgccggaac gccagcagga ccatccccgg catggcccct gaagccggtg
     2941 gcgagcacat agagttcggc cgaatcggcg cggcttgcgg ccggcttgat gtggcgaacc
     3001 accgcaaaat cgcgcttgag cgcggtcacc agcgctgccg tctcaccgcc ctggaacagc
     3061 ttgcagagaa aatagccgcc gggggacagc acctcgcgcg catactctgc cgccagttcg
     3121 gccagaccga tgatccgcag atggtcggtt ttcttgtgcc cggtggtatt ggccgccatg
     3181 tcggagagca cgccatcggc cgccccgccc agcatcgcct tcagccgctc gggcgcgtcg
     3241 gcatcgttga aatccatcac cgcgaaatcg accccggcga tcggctcgat atcgagcagg
     3301 tcgattccca ccaccttgcc gcggccgttg atactgtcga cacgtctggc cgccacctgc
     3361 gaccagccgc ccggcgcggc gccaagatcg acgatgcgct ggcccggctt cagcagatgg
     3421 tggcgatcgt cgatctcgat cagcttgtag gcggcgcggc tgcgatagcc gtcgcgcttg
     3481 gcctgggcga catagggatc attgagctgg cgttgcagcc attgctgcga ggaatgcttg
     3541 tgggcccttg cgttcttcag cctgacggca agatcgcggc tgccgccgcc tttgccaccg
     3601 cctttgcctg cctcgctcat cgcccgccct cgccccagag gccatcggcc gtcatcagtt
     3661 cgaccagaat gccttcgcgc aagccgcgat cggcaatgcg cacccgcttg cttggaaagg
     3721 cccggcggat cgcctcgaag atggcacagc cgggcagcac aagatcggcg cgctcggccc
     3781 cgatgcagcc attggccgcg cgctggtcat aggtcatgcc cagcagcgtt tgcagcaccg
     3841 tctcgacctg ccggtcgctc atccagagcc catcgacctg acgccggtca tagcgctcaa
     3901 ggccgaggtg caggccggcc aatgtcgtca ccgtgccgga cgtgccgaga aggtggaaat
     3961 tggccccctc ggtcgcccgc cgggcggacc gggcgaaccc ggccagatgc tcgctggcat
     4021 cggcaaccat cgcctcgaag ctctcaggcg tgacattgat gccgccatgc cgttcggcca
     4081 gggtcaccac gccgaccgtc agcgaggccc aatcggcgat ccgctgccgg gtgggctggt
     4141 tgtggccctg ccggccgcgc agccagacca tctcggtcga tcccccgccg atatcgaaaa
     4201 tgatcacatc ctcggcggcc ctgtcggcca gcaccgcgca gccggcggcg gctagatgcg
     4261 cctcctcccg gccatcgaga atttcgagag caagcccggt ctgctgccgc accgaggcaa
     4321 gaaattcctc gccgttgacg gcggcgcggc aggcttcggt ggtgatgaaa cgggcgcgcg
     4381 tcacgccccg gaatgcgatc ttgtcacggc agatcttgag tgcatcgagg gtccgctgca
     4441 tcgccgccgg tcgcaaccgc ttttccgaac cgaatccctc gccgaggcgc acgatccggg
     4501 aaaaggcatc gacaacgcga aacccgttcc ggctgggtct ggcgaccagc aggcggcaat
     4561 tgttggttcc caggtcgagc gccgcataca cgggatcgga ccggaccgcg ccaggcgccc
     4621 gtccttcatc ctccccgtcg tgtgttgtcc tttccgaact tggtggcggg cggcgctgat
     4681 ccccttgcgg cggatcaatc gtcatcgatg tgcctttctc gaatccgata gtaagcactg
     4741 gaccgggcct atgggaagca ttcgttcgga taagtggctg gctttgatgc aagacaaggc
     4801 gcaaaattgt cgctatcagc atcttctgcc ggggccgtat tgttcgacga ggagatctat
     4861 gtccaacgcc aatctcaagc gcatcattgt ccatcttgcc cgcaccaagg cttttcccaa
     4921 cggttcgccc aaccacggat atgagttcgt tgcgccgctg gacgcgagta accacattga
     4981 tgccgatgcg tggcacaagc tgcgcgacaa atgccgggtg acgcggttct ggggtggcga
     5041 ggaagaggat atcggtcatc tggtgcgcaa gcccggcggg tcctgggcct tccattatga
     5101 cgtgaagggc gaagaggacg acgaggccgg ctatcgcttc gccgcgcatg ccttcgtcga
     5161 gcgcgaatat gtttcgatca aggatcagga cggggaattg cagccgttcg tggtcgcctc
     5221 ggtcgaggat gcctgaccgg ctgcggcaca ggctgcccgc tacgcaaggc ggcccatttc
     5281 ggatcaaacg ggggcggcag gcccccgttg tcttgcgcta aagcatcatc ccgccaggtg
     5341 gatgccggct ggcggaaaaa gatgatgcgt tcataatagg ttagagcaaa agagcgatcc
     5401 agtcagatca gctttttgct ctaggcggtc aagccgccct ttgacgccgg ggccacaatc
     5461 ggcaataagc gcggcactcc tgcccgctct tttcgcaacg gcccggccat gtccgcttac
     5521 tcatccgact tcctgcgcgt tctgaacgag cgcggtttca tccaccagtg ctccgacttc
     5581 gccggccttg atgccagggc tgccaggggc gaggtggtgg cctatgtcgg ctatgattgc
     5641 acggcgcggt cgctgcacat cggcaatttc ctgtcgctga tgatgctcta ctggctccag
     5701 caatcgggca acaagccgat caccttgatg ggcggcggca ccacccgcat cggcgatccg
     5761 tccggcaagg acgaaacgcg cgcgctgcgc tcgatcgagg agatcgacgc caacaaggcg
     5821 gcgatcaagg gcgtgttcgc caagatcctg accttcggcg ccggcaggac cgatgcgatc
     5881 atgctcgaca acgccgaatg gctgcccgaa ctgaagtgga tcgacatgct gcgcgatgtc
     5941 ggccggcatt tctcggtcaa ccgcatgctg tcgatggatt cggtgaagct gcggctcgac
     6001 cgcgagcacg agatgagctt catcgagttc aactacatga tcgcccaatc ctacgatttc
     6061 gtcgaactga accggcgcac cggctgcaac ctgcagatgg gtggctccga ccagtggggc
     6121 aacatcatca tgggcgtcga tctcggccgc cgcatgggca ccgaacagct ctacgcgctg
     6181 acgacgccgc tgctgaccac cgcctccggc gccaagatgg gcaagaccgc caggggcgcc
     6241 gtgtggctca acgccgagga tttctcgccg tacgacttct ggcaatactg gcgcaacacc
     6301 gaggacgccg acgtcggccg cttcctcaag ctgttcacca ccctgccgat ggaggaaatc
     6361 gcccggctgg aagctttggg cggcgccgag atcaacgagg ccaagaaggt gctggcaagc
     6421 gaggcgaccg cgctgctgca tggccgcgcc gaggccgaca aggccgccga gactgcgcgt
     6481 cttgccttcg aacagggtgt cgccgcggcc g
//
LOCUS       DAOMXG010000140         6463 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_6308_length_6463_cov_8.834114, whole genome shotgun sequence.
ACCESSION   DAOMXG010000140 DAOMXG010000000
VERSION     DAOMXG010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 6463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6463
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6308_length_6463_cov_8.834114"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="PLQ11_09575"
     CDS             <1..326
                     /locus_tag="PLQ11_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="laccase domain-containing protein"
                     /protein_id="HRK25188.1"
                     /translation="ERRRIVAVLGPTISARSYEVSAEFVANFIAAEAGNQRFFIPSAR
                     SGHAMFDLPGYIVMRLKRIGVGTAVNLDICTYLNEGRLFSYRRMTHRGEPDYGRQMSA
                     IAIVP"
     gene            complement(341..1096)
                     /locus_tag="PLQ11_09580"
     CDS             complement(341..1096)
                     /locus_tag="PLQ11_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRK25189.1"
                     /translation="MKAALITGGAKRIGAAIAAALAAGGYRIALHCHRSRHEAEALAG
                     RLRLSGSEVAIVTADLADPTALPGLVADAASALGPLTLLVNNAALFEDDRIGRLDPAL
                     FDRQMAVNLRAPLLLAEAFARQAPVGADASVVNLVDQRVLRPTPDHVSYGLSKAALWA
                     ATQMMAQALAPTIRVNAVAPGPALPNWQEGEAAFARESAALPLARPVSPDHIAEAVVF
                     LAGAVSTTGQMIAVDGGQHLSWRTPDVLAREVP"
     gene            complement(1093..1887)
                     /locus_tag="PLQ11_09585"
     CDS             complement(1093..1887)
                     /locus_tag="PLQ11_09585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012452363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRK25190.1"
                     /translation="MPALPSLSIFLITRNEADRLPRTLQAIAGLSDDIVVVDSGSTDA
                     TREVAAAHGARVIVNAWPGYGPQKRFAEEQCRHPWLLNIDADEVIPADLRAEITAALA
                     GPTPADAFEIPIAEIFPAEQEPHRLAYALAPVRLYRRDKGRYSASPVHDRVELAPEAR
                     IARLNGAIHHFSVRSIGDQIAKLNAYTDQQVRDLATRGKHVPVWRLFVEFPAAFIKAY
                     LIRRHFVRGIYGFITAMNFAFYRWLRVAKDFERRMLERHSRDDNPP"
     gene            2203..2958
                     /locus_tag="PLQ11_09590"
     CDS             2203..2958
                     /locus_tag="PLQ11_09590"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HRK25191.1"
                     /translation="MAVFAVSASAADLPSRTRQPATPVVAATPAFTWTGLYAGVNAGY
                     GWSSGEKLSYRGDAASLAYAGSGVYPTGLSSTRSGFVGGGQIGANYQMGQFVAGIETD
                     LMGIGSAKRVGYTLASGTNGSFKDAMGWLGTTRVRLGYTPVNRLLVYATGGAAYGSTK
                     HHGEIVAPSLGGARWFGRDSDTRFGWALGAGGEYAITNNLIARLEYLYYDLGRSNLAL
                     AAANAPAVALGNTPLMRHENRGSIVRAGLNYKF"
     gene            complement(3202..3414)
                     /locus_tag="PLQ11_09595"
     CDS             complement(3202..3414)
                     /locus_tag="PLQ11_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HRK25192.1"
                     /translation="MAQQGTVKFFNTDKGYGFIKPEGGGADIFVHVTALQAAGLSGLK
                     EGQRISFDVEPDKKGKGPKAINLVAL"
     gene            complement(3599..4444)
                     /gene="rlmJ"
                     /locus_tag="PLQ11_09600"
     CDS             complement(3599..4444)
                     /gene="rlmJ"
                     /locus_tag="PLQ11_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HRK25193.1"
                     /translation="MNYRHAFHAGNFADVLKHALLCRILVHLAAKDTPFRVIDTHAGT
                     GVYDLTSSAAQRSPEWRDGIARLVGTHLPEEVAPLLAPYLALAAPMLSAERPTYPGSP
                     RIALALTRTQDRLSFVEKHPDDIIGLKAAIAGDRRAKALHLDGWTAWNAQVPPPERRG
                     LVLVDPPFEADSDWQRMVDGLIQSARKWAGGIVMLWYPVKDPATVERFFDALEATGIR
                     KILRLELAVDQVRPDGPLAATGVIMVNPPHRLETEARLLLPFLARRLARSRDAATCIE
                     WIVAE"
     gene            complement(4441..5109)
                     /locus_tag="PLQ11_09605"
     CDS             complement(4441..5109)
                     /locus_tag="PLQ11_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T2"
                     /protein_id="HRK25194.1"
                     /translation="MIRWFAGVLALFLMASSAAAQRFDDRRGGTPGDFDFYVLALSWS
                     PTFCAGEAGRRSRQQCEIEARDEFVVHGLWPQFERGFPSYCGAGQRFLPRPVLSKAAE
                     IFPDERLARYQWEKHGSCSGLGPSQYFDDVAAARAKVTIPAEFHRPKQPIETSPQDIE
                     RGFVAANRGLRPDMMAVSCQRGRLTEVRVCLSRDLRSFVPCPEVNRSACRSRDLTVPV
                     GRER"
     gene            complement(5106..5543)
                     /locus_tag="PLQ11_09610"
     CDS             complement(5106..5543)
                     /locus_tag="PLQ11_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25195.1"
                     /translation="MPLRLLAAGLLAVLAATGLAAREQIPAEERWWPYSAQLPGCDDP
                     AVLDRIRSRFAEKESRYWHSALEIVSVDRIRTTAFRPNGLDLIPRRYCTARVHLNNLR
                     TSALHYSVIEDAGMAGYGFGVRFCVAGYDRNWANAPECKMARP"
     gene            complement(5639..>6463)
                     /locus_tag="PLQ11_09615"
     CDS             complement(5639..>6463)
                     /locus_tag="PLQ11_09615"
                     /inference="COORDINATES: protein motif:HMM:NF018597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HRK25196.1"
                     /translation="MRSGQVRESQYVKISKSIDGPGICGANTPLKVAALASPVAPDYA
                     QSGNLILTSGNLMLTSITPTATLACPMVSAVDSWLGNDIQTAAMTWFGQPVVELVTAG
                     SFGCRPMNHRAGARLSEHSFANALDLKAVRLADGRTVSVAKGWRGAGDEQGFLREITH
                     LACKRFTTVLAPGSDALHYDHIHIDLARHNARGDRHVCRPDVPMPPPPGSQPQIAQSP
                     VMKPAPATDSQDGEIEDLRSEPNFSGRYFDVTGSVRSPDDPPVLKQHPGVDRRKPR"
BASE COUNT         1178 a         2017 c         2160 g         1108 t
ORIGIN      
        1 cggaacggcg ccggatcgtt gccgtgctcg ggccgacgat cagcgcccga tcctatgagg
       61 tcagcgccga attcgtggcg aatttcatcg cggcagaggc gggcaaccag cggtttttca
      121 tcccgtctgc ccgctccggt catgcgatgt tcgacctgcc cggctatatc gtcatgcggc
      181 tcaagcgtat cggtgtcggc accgccgtca atctcgatat ttgcacctat ctcaacgagg
      241 gtcggctgtt cagctatcgc cgcatgacgc atcggggcga acccgactac gggcggcaga
      301 tgtcggcgat tgccatcgtg ccttaaccgg tccggccgcg tcaggggact tcgcgggcca
      361 gcacgtcggg ggtgcgccag ctcagatgct ggccgccatc gaccgcgatc atctgcccgg
      421 tggtgctgac ggccccggcc agaaagacga cagcctcggc gatatgatca ggcgaaaccg
      481 gacgggcgag cggcagggcg gcgctttcgc gcgcgaaagc ggcttcgcct tcctgccagt
      541 tgggcaaggc ggggcccggc gcgaccgcgt tgacgcggat cgtcggggca agtgcctgcg
      601 ccatcatctg ggttgcggcc cataacgcag ccttggacag cccataggag acgtgatccg
      661 gtgtcgggcg cagcacgcgc tgatcgacga ggttgaccac actggcatca gccccgaccg
      721 gcgcctggcg ggcaaaggct tcggccagaa gcagcggcgc gcgcaggttg accgccatct
      781 gccggtcaaa cagcgccgga tcgagccgac cgatgcggtc atcctcgaac agggccgcat
      841 tgttgacgag caatgtcagc gggccgagcg cagaggcggc atcagcgaca agaccgggca
      901 gcgcggtagg atcggcaaga tcggccgtga cgatggccac ctcagaaccc gacagccgta
      961 gccggccggc gagggcctct gcctcatggc gggaccggtg acaatgcagc gcgatgcggt
     1021 agccaccagc ggccagagcg gcggcgatgg cagcgccgat gcgttttgcg cccccggtga
     1081 tcagcgccgc tttcatggcg gattgtcatc gcgggaatgg cgctccagca tccgccgctc
     1141 gaaatccttg gcaacccgca gccagcggta gaaggcgaaa ttcatcgcgg tgatgaagcc
     1201 gtagatcccg cgcacaaaat gacggcggat cagataggcc ttgatgaagg cggcagggaa
     1261 ttcgacaaac aaccgccaga ccggcacatg cttgccgcgc gtcgcaaggt cccgcacctg
     1321 ctggtcggta taggcgttga gcttggcgat ctggtcaccg atcgagcgga ccgagaaatg
     1381 atggatggcg ccgttcagtc gggcaatccg cgcttccggg gccagttcca cacgatcatg
     1441 caccggcgag gcagaatagc ggcccttgtc acgccggtag agccgcaccg gcgccagtgc
     1501 ataggccagc cgatgcggtt cttgttcggc aggaaaaatc tcggcaatgg ggatttcaaa
     1561 ggcatcggcc ggggtggggc cagccagtgc agcagtgatt tcagccctta gatcggccgg
     1621 gatgacctcg tcggcgtcga tgttgagcaa ccaggggtgg cggcactgct cctcggcaaa
     1681 gcgtttctgc gggccgtagc cgggccaggc attgacgatc acccgcgcgc catgggccgc
     1741 cgctacctcc cgggtcgcat ccgttgaccc ggaatcgacc acgacgatgt catccgacag
     1801 ccccgcaatc gcctgaagcg tgcgcggcag acgatcagcc tcattgcgag tgatcaggaa
     1861 aatcgacagc gagggcaggg cgggcatggc cgcttggtgg catgagtaag gcagccttgc
     1921 aagggcgacg atccggcaag accgcaaatt gtctcataat atcaggcggt tgttccgtca
     1981 ttaagggctg cgtaaggtta aatattgttc tggattgtga ttaaaatctg actgatccat
     2041 atagctttcg acatgttttc gccatgccag tctgtcaggc agtggcgcat tggctacagc
     2101 aatacatcgc cgttttgctt acagtagccg gcattcggaa agcatcacct ttccaggatc
     2161 atggagtaga gaatgaagaa catcgttttg gctagcgttt gcttggctgt ttttgctgtt
     2221 tctgccagcg ctgctgatct gccgtcccgc acgcggcagc cggcaacccc tgtcgtcgcg
     2281 gcgacgccgg cctttacctg gaccggtctc tacgctggcg tgaatgccgg ttacggctgg
     2341 agctcaggtg aaaagctgag ctatcgcggc gatgcggcct cgctggccta tgcagggtcc
     2401 ggtgtctatc cgaccggcct gtcgtcgacg cggtccggtt tcgttggcgg cggccagatc
     2461 ggcgccaact accagatggg ccagtttgtc gccggtatcg aaactgacct gatgggcatc
     2521 ggatcggcca agcgcgtcgg ctatacgctg gccagcggca cgaatggctc gttcaaggat
     2581 gcaatgggct ggcttggcac gacgcgcgtt cgcctcggat acacgccggt caatcgcctg
     2641 ctggtctatg ccacgggtgg cgccgcctat ggcagcacca agcaccatgg tgagatcgtt
     2701 gcgccgtcgc tgggcggggc ccgctggttt ggtcgcgatt ctgatacgcg gtttggctgg
     2761 gcgctgggtg cgggcggcga atacgccatc accaacaacc tgatcgccag gctggaatac
     2821 ctctactacg accttggccg ttccaatctg gctttggcgg cggcgaatgc gccggcggtc
     2881 gcgctcggca ataccccgct gatgcgccat gaaaaccgcg gcagcatcgt ccgcgccggt
     2941 ctgaactaca agttctgatc ggacagcgac cgaaagcccc ggcagtgcgc cggggccgac
     3001 gggcttgtcc cagcgcccct cgggaacgaa tccacagatc aggatcgaag gccgccggtc
     3061 aggaacgaat acaacactga gccggaccga aagcgctgct ttccgctgca ctttacaacc
     3121 aggctgctgc tgccggccac aaagtcgcgc gttctggtga agtcaccaga acacgggcga
     3181 gatcgtgcgg tcgatagggt gttacagggc gacgaggttg attgcctttg gtcccttgcc
     3241 cttcttgtcc ggctccacgt cgaaggaaat gcgctggcct tccttgagac cgctcaggcc
     3301 agctgcctgc aaagcggtca catgcacgaa aatgtcagcc ccaccacctt cgggcttgat
     3361 gaatccatag cccttgtcgg tgttgaagaa cttcacggta ccctgctgcg ccattgagcc
     3421 tgcgcctccc tcacgcgccg gagcaatttc gatgccgctc ccggtggcgc tgccccgaca
     3481 ggccctcccc tgcggtggat ggccgcgcct cctgtcgcga tcttttttca gaagtgccgc
     3541 tttggcaagt gcaattcgtt caccagtcgc cggtcagcgg cggatttggg aaaaatttct
     3601 attcggcgac aatccactcg atgcaggttg cggcgtcgcg gctgcgcgcc aggcgacggg
     3661 cgagaaaggg tagcagtagg cgggcctcgg tctccagccg gtgcggagga ttgaccatga
     3721 tcactccggt ggcggccagc gggccgtccg gccgaacctg gtccacggcc agttcaagcc
     3781 gcaggatctt gcggatgccg gtcgcctcca gcgcgtcgaa aaagcgctcg accgttgccg
     3841 ggtccttgac cggataccac agcatgacga tgccgccggc ccatttccgc gccgactgga
     3901 tcaggccgtc caccatcctc tgccagtcgc tgtcggcttc gaacggcgga tcgaccagaa
     3961 ccaggccacg acgctcgggc ggcggcacct gcgcattcca tgccgtccag ccatcgagat
     4021 ggagcgcctt ggcgcggcgg tctccggcga tggctgcctt gaggccgatg atgtcgtccg
     4081 gatgtttctc gacgaagctg agccgatcct gagtgcgggt cagggccagc gcgatgcggg
     4141 gcgagccggg ataggtcggg cgctcggccg acagcatcgg cgcggcaagg gccagatagg
     4201 gcgccagcag gggagcgacc tcttcgggca gatgcgtgcc gaccagccgg gcgatcccgt
     4261 cgcgccattc cgggctgcgc tgcgcggcgc tggaggtcag gtcatagaca ccagtcccgg
     4321 cgtgggtgtc gatcacccta aacggcgtat ccttggccgc cagatggaca agaatgcggc
     4381 acagcagggc gtgcttgagc acatcggcga aattgccggc gtgaaaggca tgccgatagt
     4441 tcatcgctcc cggccgaccg gaacggtcag gtcgcgactg cggcaggcgg agcgattgac
     4501 ctcggggcag gggacaaagc tgcgcagatc cctcgacagg cagacgcgca cctcggtcag
     4561 ccggccgcgc tggcaggaca ccgccatcat gtccgggcgc aagccgcgat tggctgcgac
     4621 aaagccgcgc tcaatgtcct gcggggatgt ttcgatgggc tgtttcggac ggtgaaactc
     4681 ggccggaatc gtgaccttgg cgcgagccgc tgccacatcg tcgaagtatt gcgaggggcc
     4741 gaggccggaa cagctgccgt gcttttccca ttggtagcgg gccagacgct catcggggaa
     4801 gatctcggcc gccttgctca gcaccgggcg cggcagaaag cgctggccgg cgccgcaata
     4861 gctcggaaag ccacgctcaa actgcggcca caggccgtga accacaaatt cgtcacgagc
     4921 ctcgatctcg cattgctggc gactgcggcg ccccgcttcg ccggcgcaga aggtgggcga
     4981 ccaggacagg gcgagcacgt agaaatcgaa atcgccgggc gtgccgccgc ggcgatcgtc
     5041 aaaccgctgg gccgccgcag agctggccat cagaaacagg gccagaacgc ctgcaaacca
     5101 ccggatcacg ggcgtgccat cttgcactcg ggcgcgttgg cccagttgcg atcatagccg
     5161 gcgacgcaga aacggacgcc gaagccgtat cctgccatgc ccgcatcctc gatgacggaa
     5221 tagtgcaggg cagaggtcct gaggttgttg agatgcaccc gggcggtgca atagcgacgc
     5281 ggaatgagat ccagcccgtt gggtcggaag gccgtggtgc ggatacggtc gacgctgacg
     5341 atttccagtg ccgaatgcca gtagcggctc tccttttcgg caaaacgcga ccggatacgg
     5401 tccagcacgg caggatcgtc acagcctgga agctgggccg aatagggcca ccagcgctcc
     5461 tccgccggga tctgctcccg ggcggcaagc ccggtcgcgg caagaacggc cagcaggcca
     5521 gcggcaagaa ggcgcaaagg catcaacctg tcccccgaac ggattcgctt cgacggttgc
     5581 ccctgctgta gcgggcgcac cgcgcactgg caagcgcagg tcctgccggg cctgaggatc
     5641 agcgcggctt gcggcggtcg acgcccggat gctgcttgag aaccggcgga tcgtcgggcg
     5701 aacggacgga gcccgtgaca tcgaaatagc ggccactgaa attgggctcg ctgcgcagat
     5761 cttcgatctc gccgtcctgg ctgtcggtcg ccggcgccgg cttcatcacc gggctttgcg
     5821 cgatctgcgg ctggctgccg ggcgggggtg gcatcggaac gtccgggcgg cagacatggc
     5881 ggtcgccgcg cgcattgtgg cgggccagat cgatgtggat atgatcgtag tgcaacgcat
     5941 ccgagccggg cgccagaacg gtggtaaagc gcttgcaggc aaggtgggtg atctcgcgca
     6001 ggaagccctg ctcgtcgccg gcgccgcgcc agcccttggc gaccgagacc gtccggccat
     6061 cggccagacg cacggccttc aggtccagcg cattggcgaa ggaatgctcg gacagacgtg
     6121 ctccggcgcg atggttcatc ggccggcagc caaaggagcc ggcggtcaca agttcgacca
     6181 ccggctggcc aaaccaggtc atggccgcgg tctggatgtc attgccgagc caggaatcga
     6241 cagcggacac catcgggcag gccagcgttg cggtcggggt gatcgaggtc agcatcaggt
     6301 tgccgctggt caggatcaga ttgccggact gggcatagtc gggcgccacc ggactggcca
     6361 gagccgccac cttcagcggt gtattggcgc cacagatccc cggcccgtcg atggatttgg
     6421 agatcttcac atactggctt tcccgcacct ggcccgagcg cat
//
LOCUS       DAOMXG010000141         6326 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_6493_length_6326_cov_10.794451, whole genome shotgun sequence.
ACCESSION   DAOMXG010000141 DAOMXG010000000
VERSION     DAOMXG010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 6326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6326
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6493_length_6326_cov_10.794451"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(714..1547)
                     /locus_tag="PLQ11_09620"
     CDS             complement(714..1547)
                     /locus_tag="PLQ11_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016919438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HRK25197.1"
                     /translation="MKVALIQTNPGDDKAANLAEAERLIEQAVAQERPDFVMLPEVFD
                     WMGSPGRDKLQACEPERDGPAYRMCQSQARKHGIWVHSGSFRTISPGQDRFANTSVVF
                     DRSGREAARYAKIHMFDITAPNGQSYRESAGCQPGDRIVTFDCDGVRVGLAICYDLRF
                     PELFQALVQQGAEIIALPAAFTLVTGMDHWEVLCRARAIETECFVLACGQTGAYQYGT
                     EKRANFGRSMIVDPWGDVIAKAAQGVGYVAGKLNLSRIAEVRRAIPVAEHKVIGRRIK
                     V"
     gene            1769..2812
                     /locus_tag="PLQ11_09625"
     CDS             1769..2812
                     /locus_tag="PLQ11_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009210401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HRK25198.1"
                     /translation="MRAIVLHGPHDLRIEPFQDLPPAPAAGEVRVQMAAGGICGSDLH
                     YYHHGGFGVVRVKQPMALGHEASGRVVALGAGVSGLKRGQLVAVNPSVPCNNCDYCRR
                     GLRNHCENMMFNGSAMRFPHVQGMFREFMDVTADRAFAMPEAIVPEEAALCEPLSVVL
                     HAANQAGDLQGRSVLVSGCGPIGVIAVAVARLRGAARIIATDITPHSLEIAEKLGADE
                     TINVATSAERLAAYATGKGTLDVVFECSGAAPAIAAALATLRPKGRMVFVGLGGDAPL
                     NVNMVVAREIELVGTFRFDAEFAEAAHLIGTRTLDLSAAISATYPFGEAVAAFEHAGD
                     RTRATKVAINLEG"
     gene            complement(2820..4208)
                     /locus_tag="PLQ11_09630"
     CDS             complement(2820..4208)
                     /locus_tag="PLQ11_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRK25199.1"
                     /translation="MARVAQSESDTNLTGRRAEWQARVLDGKARDILARDERYFLRQS
                     VSTPCLNAIARAEGIWIEDTAGRRYMDFHGNNVHHIGYGHPRLKAAIARQMDELPFAP
                     RRYACDTAARLAEKLAAIAPASLSKLLFTTGGSDANEVAIKVARIATGRFKTLSFWDA
                     FHGAGFGAGALSGEALFRSGPAAPLVAGASHVAPFGCYRCPYGHPVDGAGQPDLARCG
                     LACARMVEYVLEREGDIAALIAEPARAVPFLPPPGYWKAVRDACHRHGTLLIFDEIPT
                     GLGKTGRMFACEHDEVVPDILVMGKALGGGILPIAACITRPELDVAGEWAFGHYTHEK
                     NPVTTRAALTTIEIIEDEGLVENAARVGRAALARLDAMKSRNAAIGDVRGRGLLIGIE
                     MVRDRTSKEPDRDLAEAVMYGALDRGLSFKTTMGNVLTLTPPLTVTEAEMMQALAIID
                     EAIGEATRRRSA"
     gene            4360..4971
                     /locus_tag="PLQ11_09635"
     CDS             4360..4971
                     /locus_tag="PLQ11_09635"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRK25200.1"
                     /translation="MLDGAIRRMIVPALDKMGAGLARRGVSANRVTLLGLAASLAMGV
                     AIAVGWYWSALGLLGLSRLCDGLDGAVARHRGLSDFGGFLDIVCDFAFYAALPVGFAL
                     ASPSEYALPATVLLASFYLNGASFLAFAAIAARRGLESTLRGPKSLYFTTGLAEGSET
                     IAVFILMLAWPAAFSLLAYGFAGLCLVTCLGRIVFAFRTFSGG"
     gene            5054..6142
                     /locus_tag="PLQ11_09640"
     CDS             5054..6142
                     /locus_tag="PLQ11_09640"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006933591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
                     /protein_id="HRK25201.1"
                     /translation="MSYPHGIFSDAMIERLVASGAITSHAAPVAGQIQPASMDLRLGQ
                     RCWRVRASFLPGPDRAARRRIDSLSLHEIDLSRGAVLETGCVYVAELMESLALPAEVA
                     ASANPKSSTGRIDVFTRVIVDGGSAFDQIPAGYRGPLYAEISPKTFPVLVRSGSRLSQ
                     IRFRSGQPRLDDATLAALDTEAQLIAGGRHPIAGGVAVSVDLAGFTPDGLIGYRGKRH
                     TGVIDVDRVGVHRIADYWEPLMREETRALVLDPSEFYILASREAVHVPPGYAAEMVAI
                     DPNVGEFRVHYAGFFDPGFGHAAAGGHGARAVLEVRSHNVPFIIEDGQIVGRLVYEKM
                     AEQPRVLYGADLQSNYQAQGLKLSKHFR"
BASE COUNT         1096 a         1933 c         2024 g         1273 t
ORIGIN      
        1 tcgcaaccgg gccttcggcc ctgctcggcg tcgcattcgc tcctgaccag cggccctgac
       61 aattgcccct aacggtcaat tgtcaggacc gctggtatga cttgcctggg tgccggggtg
      121 gggcgacccg gatgaccgaa gctttcacat tgtgaaacac aattgcagaa ttaacctctg
      181 tatagcggtc agattgacca aagggcaatt aaataaatac catcacattt acattgatgg
      241 aggaggtcat gggtgatgta gtgggctcaa taaataaaaa tggtcctata gtaggcgatt
      301 gcaaagatag taacatgact tatgatgttg gcaattttgc tgtcttgttt cttggcgatt
      361 caaacgaatt gtcaagaaaa atggcaatct ctgccacgac gggcacaatg cgggcttggt
      421 gcgtacgtgg ctctggccag gaatggtgct cgtcaccgga ccaatgccat aaccgcggga
      481 gcaatccggc ctatgcctgc ttcctcgatg tatcgcactg tgaccccgaa ctgttgccgt
      541 tggaggctct ggtggcaacc cgtctctacg gatttgccgg gctctgcatg cccaaggcaa
      601 aggaagggtg cgcgaccgcg caaacggtaa ctcagctcag ccagaaaacc gggctgccaa
      661 ttctgtttgt gttcaatgat ccggcagcag ccggcgcccc ggaaaccgtt gcgtcagacc
      721 ttgatgcgtc tgccgatgac cttgtgctcg gcaaccggta tagcgcgcct gacctcggcg
      781 atgcgggaga ggttcaattt gccagcgacg tatccgacac cctgcgccgc ctttgcgatc
      841 acgtcgcccc acggatcgac gatcattgag cggccgaaat tggcgcgctt ttccgtcccg
      901 tattgatagg ccccggtctg gccgcaggcg agtacgaagc attccgtctc gatggcgcgg
      961 gcacggcaca gcacttccca atggtccatc ccagtcacca gcgtgaaggc cgccggcagt
     1021 gcgatgatct ctgcgccctg ctgaaccagg gcctgaaaca attcgggaaa ccgcagatcg
     1081 tagcagatcg ccaggccaac tcttacgccg tcacaatcga aggtgacgat gcgatcgcct
     1141 ggctgacagc cggctgattc gcgatagctc tggccattcg gcgccgtgat gtcgaacatg
     1201 tggatcttgg catagcgcgc agcctcccgt ccgctgcgat cgaacaccac actcgtattg
     1261 gcaaagcgat cctgaccggg gctgatggtg cgaaagctgc cggaatgaac ccagattccg
     1321 tgtttgcgcg cctgcgactg gcacattcga taggcgggcc cgtcgcgctc cggctcgcag
     1381 gcctgcagct tgtcgcggcc aggcgagccc atccagtcga aaacctccgg caacatcacg
     1441 aaatccggtc gctcctgggc caccgcctgt tcgatcaggc gctcggcttc ggcaaggttg
     1501 gccgccttgt catcgcccgg attggtctgg atcagtgcga ctttcatggc gtgtcgtatc
     1561 cttgccgctc cggtcgccga attggccacg gagaggctcc agcctgccgg gaacgccggc
     1621 aaatttcaag cgggtgccac cggcgtcata gggccgggcg cgggagggac cggggccggg
     1681 gttggcgcgg tcggaaggcc tgttcccgat catagcattc cctgtatgct gccggcagga
     1741 ctgactgttc ttacacaagg tttctgtcat gcgcgccatt gtccttcacg gaccccacga
     1801 tctcaggatc gagccgtttc aggatctgcc accggctccg gcagcgggcg aggtgcgggt
     1861 gcagatggcc gccggcggca tctgcggctc tgatctgcat tattatcatc atggtggctt
     1921 cggtgttgtg cgcgtcaaac agccgatggc gctggggcat gaagcctccg ggcgggtcgt
     1981 cgcgctcggg gcaggcgtga gtggtctgaa acgaggtcag cttgttgcgg tcaatccgag
     2041 tgttccgtgc aacaactgcg actattgccg gcgcggcctg cgcaaccatt gcgagaacat
     2101 gatgttcaac ggctcggcca tgcggtttcc gcatgtgcag ggcatgtttc gcgaattcat
     2161 ggatgtgacg gccgatcgtg ccttcgccat gccggaagcg atcgttccgg aagaggcggc
     2221 gctgtgcgag ccgctgtcgg tcgtccttca tgccgccaat caggccggcg accttcaggg
     2281 ccgcagcgtg cttgtctcgg gctgcggccc catcggtgtg attgctgtgg cggtggcgcg
     2341 gttgcggggt gcggcgcgca tcattgccac cgacatcacg ccgcattcgc tggagattgc
     2401 cgaaaagctg ggggccgacg agacaatcaa tgtcgcgacc agtgccgagc gccttgccgc
     2461 ctatgccacc ggcaagggca cgctggatgt cgtgttcgaa tgctcaggcg cagctccggc
     2521 gattgccgcc gcgcttgcga cgttgcgccc gaagggcagg atggtgttcg tcggcctcgg
     2581 cggcgatgcg ccgctcaacg tcaacatggt ggtcgcccgc gagatcgaac ttgtcggcac
     2641 cttccgcttc gacgcggaat tcgccgaggc tgcccacctg atcggcacgc ggaccctcga
     2701 tctcagcgcc gcgatttcgg cgacctatcc cttcggcgag gcggttgcgg ccttcgaaca
     2761 cgccggtgac aggacccggg ccaccaaggt ggcgatcaat ctggaaggct gaacggagat
     2821 cacgcggacc gccgccgggt tgcctctccg atcgcctcgt cgatgatggc gagcgcctgc
     2881 atcatctccg cctccgtcac ggtcagcggc ggggtcagtg tcagcacatt gcccattgtg
     2941 gtcttgaacg acaggccgcg gtcgagcgcg ccatacatca cggcctcggc aagatcgcgg
     3001 tcgggttcct tgctggtgcg gtcgcgcacc atctcgatgc cgatcagcag gccgcgtccg
     3061 cgtacatcgc cgatggcggc attgcgggac ttcatcgcat cgagtcgcgc aagtgcggcg
     3121 cggccgaccc gcgcggcatt ctcgaccaag ccttcgtcct cgatgatctc gatggttgtc
     3181 agcgccgcgc gcgtcgtcac cgggttcttc tcgtgggtgt agtggccaaa agcccattct
     3241 cccgccacat cgagttcggg ccgggtgatg caggcagcaa tcggcaggat gccaccgccg
     3301 agtgccttgc ccatcaccag aatatccggc accacctcat catgttcgca ggcgaacatg
     3361 cggccggtct tgcccaggcc ggtggggatt tcgtcgaaga tcagcagggt gccgtggcgg
     3421 tgacaggcat cgcgcacggc tttccaataa ccaggcggtg gcaggaacgg cacggcccga
     3481 gccggttcgg cgatgagcgc cgcgatatct ccctcacgct caagcacgta ttccaccatc
     3541 ctggcgcagg caagaccgca gcgggcgagg tcgggctggc cggctccatc caccggatgg
     3601 ccatagggac agcgatagca gccgaacggt gcgacatggc ttgcgcccgc caccagcggc
     3661 gccgccggcc ccgagcggaa cagcgcctcg cccgacagcg cgccggctcc gaaaccggcg
     3721 ccatggaacg catcccagaa tgacagcgtc ttgaaccggc cggtggcaat gcgcgccacc
     3781 ttgatcgcca cctcgttggc gtccgatccc ccggtggtga acaacagttt ggacaggctt
     3841 gccggtgcga tcgctgccag cttctcggca aggcgcgctg cggtatcgca ggcataacgg
     3901 cgcggggcga acggcagctc gtccatctgc cgggcaatcg ccgccttcag ccgcggatgg
     3961 ccatagccga tgtggtggac gttgttgccg tggaaatcca tatagcgccg gccggcggta
     4021 tcctcgatcc agatgccctc ggctctggcg atggcgttga ggcatggggt ggacaccgac
     4081 tggcgcagaa aataccgctc gtcgcgggcc aggatgtccc gggctttgcc atcaagcacc
     4141 cgtgcctgcc attccgcccg gcggccggtc aggttggtat cgctttccga ttgggcgacc
     4201 cgagccattg agtttgtcac gaaccaccct tccatgttga atcagtttat gacaccagca
     4261 ggtcatcagg ggatcggtgc tccacaaaat caagcgccga tttcggctgg cggcgcgggc
     4321 gccggtgagc gccgcgggcg caacgggaag gagaggagcg tgctggacgg agccattcgc
     4381 cgaatgatcg taccggcgct ggacaagatg ggagccggcc tggcgcgacg tggtgtcagc
     4441 gccaatcggg ttacactgct gggcctggcc gcatcgctcg cgatgggggt ggcaattgcc
     4501 gtcggctggt attggtcggc actgggcctg cttggcctga gccggctttg cgacgggctg
     4561 gacggagccg tcgcccggca caggggcctg agtgactttg gcggctttct cgacatcgtt
     4621 tgcgactttg ccttctatgc cgccttgcct gttggtttcg cgctggcctc gccgtctgaa
     4681 tatgcgctgc cggcaacggt gctgctcgcc agcttttacc tcaacggtgc aagctttctg
     4741 gcctttgctg caatcgccgc ccggcgtgga ctggagagca ccttgcgcgg ccccaagagc
     4801 ctctacttca ccaccggcct ggctgaagga agcgaaacca tcgcggtttt catcctgatg
     4861 ctggcttggc ctgcggcctt ttcactgctg gcctacggat ttgccgggct ttgtcttgtc
     4921 acttgccttg ggcggattgt attcgccttc cggacgttta gcggtggatg acgcgcgccg
     4981 gcgacaaggc ggtgcttgca gcgatcggca gccggaggct aggcgtagaa ggtccaagat
     5041 tgcgggaacc ggcgtgtcat atccccacgg cattttctct gatgcgatga tcgaaaggtt
     5101 ggttgcctcg ggtgcgatca cgtcccacgc ggctccggtc gccgggcaga tccagccggc
     5161 cagcatggac ctgcggctgg gtcagcgctg ctggcgggtc agggccagtt ttctgcccgg
     5221 ccccgaccgg gcggcgcgca ggcggatcga cagtctcagc ctgcacgaga ttgacctgag
     5281 ccgcggtgcg gtgttggaaa ccggctgcgt ctatgtcgcc gaactgatgg aaagccttgc
     5341 cctgccggca gaggtcgccg cctcggccaa cccaaagagt tcgacgggtc ggatcgacgt
     5401 attcacccgc gtgatcgtcg atggcggcag tgctttcgac cagattccgg cgggctatcg
     5461 cgggccgctt tatgccgaga tttcgcccaa gacctttccg gttcttgtgc gttccggctc
     5521 acgtctgtcg cagatccgct tccgctccgg gcagccccgg ctcgatgatg ccacactcgc
     5581 cgccctcgac accgaggcgc agctgatcgc cggcggccgc catccgattg ccggcggcgt
     5641 ggctgtcagc gtcgatcttg ctggcttcac cccggatggc ctcatcggct atcgtggcaa
     5701 gcgccatacc ggcgtgatcg atgtcgatcg cgtcggcgtc catcgcatcg ccgattattg
     5761 ggagccgctc atgcgcgagg agacgcgcgc attggtgctt gatccgagcg aattctacat
     5821 ccttgcttcg cgcgaggcgg tgcatgtccc gccaggatat gcggcggaga tggtggcgat
     5881 cgaccccaat gttggcgagt ttcgcgtgca ttatgccggt ttttttgatc ccggctttgg
     5941 ccatgccgct gctggggggc atggagcccg cgcggttctg gaggtgcgct cgcacaatgt
     6001 gccgttcatc atcgaggacg gtcagatcgt cgggcggctg gtctatgaga agatggccga
     6061 acagccacgg gtactttacg gagccgacct gcaatccaac tatcaggcgc agggactgaa
     6121 actttccaag cattttcgct agatcatgct ctcgttttga ttagagcatt ttcaagcgaa
     6181 gtggagtccg gttcgcgtaa agaaaatgcg aaaaaacaaa aagatagagt gcactctgtg
     6241 aattggaatt cactgaaagt gctctaaggc ccgtggggat tcatcgtgaa ttgatgacgg
     6301 ctgctgcgat gtagatcatt gcttcg
//
LOCUS       DAOMXG010000142         6304 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_6530_length_6304_cov_11.420867, whole genome shotgun sequence.
ACCESSION   DAOMXG010000142 DAOMXG010000000
VERSION     DAOMXG010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 6304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6304
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6530_length_6304_cov_11.420867"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(211..1080)
                     /locus_tag="PLQ11_09645"
     CDS             complement(211..1080)
                     /locus_tag="PLQ11_09645"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRK25202.1"
                     /translation="MLRIIQGGGAIQREAGGAWPHPTARNVPSRRGTQAVERALDLLV
                     QVAADDGTGQALGEIAASTGLDAGTARRLLSALVARGFVDQDRLTRRYHLGIEVFTLA
                     AAASNRLDFGGVCRDALLRMSRLSGCDAALMIGAGGDLICLDLVRAAEDRSTRDLSIG
                     DRRLIGGDAFGIAILAGMSDQQAEDIVIGNIRALTPEPEQGVVMIREHLQAARRDGYC
                     LWRRPLSGRGALAVAVADHEGRPVGAFGVSLPDAGDVPAEMLGLQAQDLRLEARRIEE
                     VLWRDSDGKGRGQ"
     gene            complement(1261..1962)
                     /locus_tag="PLQ11_09650"
     CDS             complement(1261..1962)
                     /locus_tag="PLQ11_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003498150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRK25203.1"
                     /translation="MLEVTGLVSAYGRVEVLHGVAITVDKGEIVTLVGANGAGKTTLL
                     RCISGVQPMRAGTIRFDGQPIERMPAHRRVGLGLAQVPEGRHVFGPVSVEDNLRLGAY
                     TRSDRDIDADLEKIYDLFPILKEKRLLHAAGLSGGQQQMLAMGRAMMSRPRLLLLDEP
                     SMGLAPVIVEQIFGVIRKLAAEGVTILLVEQNAFAALSMADRGYVVETGRIAFTGSGK
                     DLLVDPRVREAYLGL"
     gene            complement(1955..2743)
                     /locus_tag="PLQ11_09655"
     CDS             complement(1955..2743)
                     /locus_tag="PLQ11_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018856758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRK25204.1"
                     /translation="MSLLEVSKVGIAFGGIKAVDQVSLSVQPGEIYSIIGPNGAGKTT
                     LFNLISGVYHTQSGAIRLAGHDVTGLRPYDLARKGMSRTFQNLQVFTRMTALENVMTG
                     RHLHEQHGIIGHMLHLPATIAQERRSRAAALACLDRVGLADRADDRAGSLSYGAMKRL
                     EIARALATEPKILLLDEPAAGCNAAETAEIDHLLVEIAKAGLSIVLIEHDMKLVMGIS
                     DRVLVLEQGRFLVEGLPAEVARDPKVIKAYLGTEAASLTEGGHA"
     gene            complement(2740..3681)
                     /locus_tag="PLQ11_09660"
     CDS             complement(2740..3681)
                     /locus_tag="PLQ11_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003498146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRK25205.1"
                     /translation="MSAISPRYRTLLAILALVILPGLVMPSTFYYRIGALVFISAIAV
                     IGLNLLMGYAGQVSLGHAGFFAIGGYACAILPARYGTDPLLAALAGIVLAGLLAFAVG
                     RPILRLRGHYLAVGTLGLGLLIGMVLTNEVWLTGGPDGMQVQRATVFGVRLRSAEHWY
                     WISGTIMVLCAWIAANLFDSPTGRALRALRDSMVAAEVMGIRPSRYKLIVFVISAVYA
                     AIAGTMLALFNGLVTPDNASFLHSVELVTMVVIGGMGSLIGSIVGAAVLIILPQVLTI
                     FHEYETLLLGILIILFMLFLRQGIAPGLAGLFKGRRA"
     gene            complement(3683..4555)
                     /locus_tag="PLQ11_09665"
     CDS             complement(3683..4555)
                     /locus_tag="PLQ11_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRK25206.1"
                     /translation="MGDLLQFAVSGLTVGSVYALVALGFTLIYNASEVVNFAQGEFVM
                     IGGMATVFLYALGLPLPVAALLAVVLAVVVGLMLHTFVIAPARGADAVTLIILTIGAS
                     LVIRGAVPILYDKQFHKLPGFSGEDPIRLFGAAIQPQAFWVIGGMILVVLALHFFLNH
                     TRFGRAVIATSVSPMAAKLVGINTALVVGFSFAVSAGVGALAGILVTPVTLTSYDVGT
                     ILALKGFAAAVLGGIGSPVGAVLGGLLVGMLEALSAGLISSSYKDAAAFITIILVLLV
                     MPNGLAGLKHIERV"
     gene            complement(4634..5794)
                     /locus_tag="PLQ11_09670"
     CDS             complement(4634..5794)
                     /locus_tag="PLQ11_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRK25207.1"
                     /translation="MSIVKRLAAAAAGASLAVLAAALPASAAELRIGAFVSATGPASF
                     LGEPEEKTIKHYVEEINKSGGINGDTIKLFIYDDGGDANKARTFATRLIEEDKVVAVV
                     GGSTTGTTMAAVPVFEDAQVPFISLAGAVVIVEPVKKFTFKTPHTDRMACEKIFDDMK
                     KRGLTKIGMISGTDGFGKSMRDECVKVNAKFGVEILGEETYGPRDSDMTAQLTNLKGK
                     AGMQALINPGFGQGPAIVTRNAKQLGINVPLYQSHGVASKSFIDLAGDAAEGVRLPAA
                     ALLVADKLADSDPQKKIALGYKTTYEKVTGQPVSTFGGHAYDGLMILVEAMKRAKGAD
                     PKKIRDEIEATKGFIGTAGIFNMSATDHMGLGLDAFRMLEIRKGDWTLADAK"
     gene            complement(5855..>6304)
                     /locus_tag="PLQ11_09675"
     CDS             complement(5855..>6304)
                     /locus_tag="PLQ11_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaN"
                     /protein_id="HRK25208.1"
                     /translation="AATGRVVRASGPVDGMAGARSATPLIVALKAADEAVYLEEWFGP
                     ISFVIATASTDDSLARAAGSMRRHGAITAALYSTSDVVIAAAEEAFAGAQVALSINLT
                     GNIFVNQSAAYSDFHVTGGNPAGNACLTDAAFVANRFRVAPSRRLVG"
BASE COUNT         1282 a         2078 c         1917 g         1027 t
ORIGIN      
        1 cgagggacgg cccgggaaat gaccggcagg tcatttgtga gggccactgg tatcatagca
       61 gcggtcctca ggattgaccg gtctgcattc ggcggatcgg ttcagggtcg gaccgaaatc
      121 ccccgcgtaa gctcaggcgc ccgttcgggc ttgcagtggt cagtggtcat cggtcaaagc
      181 cgatgaccac cggcatcagc cggttcgtga ttactgcccg cgccctttcc catcggagtc
      241 gcgccacagc acttcctcga tgcggcgtgc ctcgaggcgt agatcctggg cctgcaggcc
      301 gagcatctca gcaggcacat cgccagcgtc cggaaggctg acaccgaagg cgccgacagg
      361 gcggccttca tgatcggcta cggctactgc caaggcgccc cgaccggaga gcggacggcg
      421 ccacaggcag tatccgtcgc ggcgggccgc ctgcagatgc tcgcggatca tcacaacacc
      481 ctgctccggt tcaggggtca gggcgcggat gttgccgatc acgatgtctt cggcctgctg
      541 atcggacatg ccggcaagta tggctattcc aaatgcgtcg ccgccgatca gccggcgatc
      601 tccgatcgaa aggtcgcgag tggatcggtc ctccgcggcg cgcacaaggt cgaggcagat
      661 gagatcaccg cctgcgccga tcatcagcgc tgcgtcacat cccgaaaggc gtgacatccg
      721 caggagagca tcacggcaca cgccgccgaa atcgaggcga ttggaggctg cggccgcaag
      781 cgtgaacact tcaatgccaa ggtgatagcg tctggtgagg cgatcctggt cgacaaagcc
      841 acgcgcaacc agcgcgctga gaaggcgccg ggcggtgccg gcatcaagac cggttgatgc
      901 cgcgatctcg cccaatgctt gccctgtccc atcgtcggcc gccacttgaa caagaagatc
      961 aagcgcccgt tcgactgcct gtgtcccgcg acgggaaggg acgttgcgag cggtgggatg
     1021 gggccatgcc ccgccagcct cgcgctgtat cgctcccccg ccctggataa tccggagcat
     1081 ggcgccagtc ctgagtccgg gccgttagaa ccgctttgcc ggattgacga aaattgcatc
     1141 aatccgcctg attaggcggc cggcccggac aaaaccgggc ggcacgcttg ccagtcagct
     1201 aacccactgc ggcaaaagcc agcggttcgg gcagggtcct ggttgcagat gatgcgcgga
     1261 tcacaggccg agataggctt cgcgaacgcg cggatcgacc aggaggtcct tgccggagcc
     1321 ggtaaaggca atccgcccgg tttccacgac atagccgcgg tcggccatcg acagggcagc
     1381 aaaggcgttc tgctcaacca ggaggattgt caccccttcg gccgccagct tccggatgac
     1441 accgaagatc tgttcgacga tcaccggcgc aagccccatg ctgggctcgt ccagcaacag
     1501 aagacgcggc ctcgacatca tcgcccgccc catggccagc atctgctgct ggccaccgga
     1561 caggcccgct gcatgcagaa gccttttctc cttgaggatc gggaaaaggt cgtagatttt
     1621 ctcaagatcc gcatcaatgt cccggtcgga gcgggtgtag gccccgaggc gcagattgtc
     1681 ctcgaccgag acagggccaa acacgtgcct tccctccggc acctgcgcca atccgagccc
     1741 gacccggcga tgggctggca tgcgctcgat cggctggcca tcgaagcgga ttgttccggc
     1801 gcgcatgggc tgaacgccgg agatgcagcg cagaagcgtt gtcttgccgg caccattggc
     1861 gccgacaagc gtgacgatct cgcccttgtc gacggtgatc gccacgccat gcaatacctc
     1921 gacccggcca taggcactga ccagacccgt cacttcaagc atggccgccc tccgtcagcg
     1981 aagcggcctc ggtgccaaga taggccttga tgacctttgg atcacgcgcg acctcggccg
     2041 gcaggccctc caccagaaac cgtccctgtt ccagcaccag aacgcgatcg gaaatgccca
     2101 tgaccagctt catgtcatgt tcgatcagaa caatggacag gcccgccttg gcgatttcga
     2161 ccagcagatg gtcgatttcc gccgtctcgg ctgcgttgca gccggctgcc ggctcgtcga
     2221 gcaacaggat cttcggctct gtggccagcg cccgggcaat ctcgaggcgc ttcatcgccc
     2281 cataggacag gcttcccgcc ctgtcatcgg cccgatcggc cagcccgacc ctgtcgagac
     2341 aggccagcgc ggcagcgcgc gagcgccgtt cctgcgcgat ggttgcaggc agatgcagca
     2401 tgtggccgat gatgccgtgc tgctcgtgca ggtggcggcc ggtcatgaca ttttccagcg
     2461 ccgtcatgcg ggtgaacacc tgcaggttct ggaaggtgcg cgacatgccc ttgcgcgcca
     2521 gatcataggg gcgcaggccg gtcacatcat gcccggccag ccggatcgcc cccgactggg
     2581 tgtggtagac gcccgaaatc agattgaaca atgtggtctt gcccgcgcca ttggggccga
     2641 tgatcgaata gatctcaccc ggctgtaccg acagcgagac ctgatcaacc gccttgatcc
     2701 cgccaaaggc aatgccaacc ttgctgacct ccagaaggct catgcccgcc tccccttgaa
     2761 caggccggca agccccggcg cgatgccctg tcgcaggaac agcatgaaca ggatgatcag
     2821 gatgcccagc agcagcgtct catactcgtg aaatatggtc agcacctgcg gcaggatgat
     2881 cagaaccgcc gcgccgacaa tcgagccgat cagcgaaccc atgccgccga tgaccaccat
     2941 cgtcaccagt tccaccgaat gcaggaagct ggcgttatca ggtgtcacca gcccgttgaa
     3001 cagcgccagc atggtgccgg caatcgccgc atagacggca gagatgacaa agacgatcag
     3061 cttgtagcgc gacgggcgga tgcccatcac ctcggctgcc accatggaat cgcgcaaggc
     3121 gcgcaaggcc cgcccggtcg ggctgtcgaa caggttggcg gcgatccagg cacaaaggac
     3181 catgatggtc ccggatatcc agtaccagtg ctcggccgag cgcagcctga ccccgaacac
     3241 cgtggcccgc tgaacctgca tgccatccgg gcccccggtg agccagactt cgttggtcag
     3301 aaccatgccg atcagaagtc caaggccgag ggtgccaacg gcaagataat ggccgcgaag
     3361 gcgaagaatg ggccggccga cggcaaaggc cagcaacccc gccagcacaa tccccgccaa
     3421 ggcagccagc agcggatcag tgccataacg cgccggcaga atggcacagg catagccgcc
     3481 aatggcgaaa aacccggcat ggccaagact gacctgcccc gcatagccca tcagcaggtt
     3541 aaggccgatc accgcgattg ccgagatgaa caccagcgcc ccgatccggt aatagaaggt
     3601 cgagggcatg accagtccag gcaggatcac caacgcaagg atagccagca atgtccggta
     3661 acggggggag atcgcgctca tctcacaccc tctcgatatg cttgagccca gccaggccat
     3721 tgggcatcac cagcagcacc aggatgatgg tgatgaaggc cgcagcatcc ttgtaggacg
     3781 acgaaatcaa cccggccgac agggcttcca gcataccgac cagcaaccca ccgagcacgg
     3841 cgccaaccgg actgccgatg ccgccaagca ccgccgcggc aaaccccttg agcgccagaa
     3901 tcgtgccgac gtcatagctg gtcaacgtca cgggtgtgac cagaatgccg gccagcgcac
     3961 ccacgcccgc cgatacggca aaggaaaatc cgaccaccag agccgtgttg atgccaacca
     4021 gcttggccgc catggggctc accgatgtcg caatcaccgc cctgccaaag cgggtatgat
     4081 tgagaaagaa atgcagcgcc aggacaacca agatcatccc gccgataacc cagaacgcct
     4141 gtggctggat cgccgcacca aacaggcgga tcgggtcctc gccggaaaag cccggcagct
     4201 tgtgaaactg tttgtcatag agaatcggca ccgcaccgcg gatcacgagg gaggcgccga
     4261 tggtcaggat gatcagcgtc accgcatccg ccccgcgcgc cggcgcgatg acaaaagtgt
     4321 gcagcatgag gccgaccacg actgccagca cgaccgccag cagcgccgcc acgggcaggg
     4381 gcagccccag cgcgtagaga aaaaccgttg ccatgccgcc gatcatgacg aattcgccct
     4441 gagcgaaatt caccacctcg gaggcgttgt agatcagcgt gaaaccgaga gcgaccagag
     4501 catagacaga gcccacggtc agcccggaca cggcaaattg caaaagatct cccatggcgc
     4561 tcccctggtc cgcgcgcatc tgtccccgtg ctggcagccg ccggacggta tccggcggcc
     4621 ggccaatccg atgttacttg gcgtcggcga gtgtccagtc acccttgcgg atttccagca
     4681 tgcggaaagc gtcaaggccg agacccatgt ggtcggtcgc actcatgttg aagatgccgg
     4741 cggtgccgat gaagcccttg gtcgcttcga tttcatcgcg gatcttcttc ggatcggcac
     4801 ccttggcgcg cttcatcgcc tcgacgagga tcatcagacc gtcataggca tggccgccga
     4861 aggtcgagac cggctgtccg gtgaccttct cgtaggtggt cttgtagcca agcgcgatct
     4921 tcttctgcgg atcactgtcc gccagcttgt cagccaccag cagcgcagcc gccggcagac
     4981 gcacgccctc ggctgcatca ccggccagat cgatgaagct cttcgaagcg acgccatggc
     5041 tctggtagag cggcacgttg atcccgagct gcttggcatt gcgggtgacg atcgccgggc
     5101 cctggccaaa gccggggttg atcagcgcct gcatgccggc cttgcccttg aggttggtga
     5161 gctgggcggt catgtcggaa tcgcgcggcc cgtaggtttc ctcgccgagg atctcgacgc
     5221 cgaatttggc attcaccttg acgcattcat cgcgcatcga tttgccgaac ccgtcagtgc
     5281 cggagatcat tccgatcttg gtcaggccgc gcttcttcat gtcgtcgaag atcttctcgc
     5341 aggccatgcg atcggtatgc ggggtcttga aggtaaactt cttgaccggt tcgacaatca
     5401 caaccgcgcc ggccagcgaa atgaacggca cctgtgcatc ctcgaacacc gggaccgctg
     5461 ccatggtcgt tcccgtggtc gagcccccga caaccgccac caccttgtct tcctcgatca
     5521 ggcgcgtggc gaaggtgcgc gccttgttgg cgtcgccacc atcgtcataa atgaacagct
     5581 tgatcgtatc accgttgata ccaccgctct tgttgatctc ctcaacgtag tgcttgatgg
     5641 tcttttcctc cggctcacca aggaatgacg ccgggccggt ggccgacacg aaggcgccga
     5701 tgcggagctc ggcagcagag gccggcagag ccgccgcaag gacggcaagc gatgcgccgg
     5761 ccgcagcggc cgcgagacgt ttgacaatgc tcatggtgtt cctcctcgtt agtcttccgg
     5821 cacagggtcc tcccctgccc tcgttcagtc aagtctatcc gaccagtcgt cgcgaaggcg
     5881 ccacgcggaa gcgattggcg acaaaggcgg catcggtcag gcaggcattg ccggcgggat
     5941 tgccgccggt cacatggaaa tcggaatagg ccgccgactg gttgacgaag atgttgccgg
     6001 tcagattgat cgacagcgcc acctgcgccc cggcaaaagc ctcctctgcc gcagcaatca
     6061 ccacatcact ggtcgagtag agcgcagcgg taatcgctcc gtgccggcgc atcgaccccg
     6121 cagcgcgcgc aagcgaatcg tcggtcgaag cggtggcaat cacgaacgag atcggcccga
     6181 accattcctc aagatagacc gcctcgtcgg ccgccttgag tgcgacaata agcggcgtgg
     6241 cgctgcgcgc gccggccatg ccgtccaccg gcccggaggc gcgaaccacc ctgccggtcg
     6301 cagc
//
LOCUS       DAOMXG010000143         6296 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_6537_length_6296_cov_9.700369, whole genome shotgun sequence.
ACCESSION   DAOMXG010000143 DAOMXG010000000
VERSION     DAOMXG010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 6296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6296
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6537_length_6296_cov_9.700369"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /locus_tag="PLQ11_09680"
     CDS             <1..1029
                     /locus_tag="PLQ11_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRK25209.1"
                     /translation="RCDVYKLHLGGGFGRRGRADYVTQAVNIARQMPGVPIKLLWSRE
                     EDMLHGTYHPITQCKLVGGLDRDGNLVGLHMRLSGQSILAGVNPQGLQNGRDPIAFQG
                     LNAGGPEGHLGYAIPNLLIDHAIRNPHVPPGFWRGVNLNHNAIYLECFIDELAHAAGV
                     DPLAFRQKMMGPYPKHRAVLNAVAEGIGWSKPAPAGVSRGLAQIMGFGSYVAAAAEVS
                     VEASGSIRVHRIVAATNPGHVVNPQQVAAQVEGSFVYGLSAALMQQCTVKNGRIVEEN
                     FDTYPALRIAEMPKVESLLLPSGDFWGGVGEPTIAVAAPAVLNAVFAATGKRVRSLPI
                     RTAELKRA"
     gene            1026..1469
                     /gene="soxX"
                     /locus_tag="PLQ11_09685"
     CDS             1026..1469
                     /gene="soxX"
                     /locus_tag="PLQ11_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HRK25210.1"
                     /translation="MMRPLFLAGCLALAALPVGAVEPEAIVVREGGIPASLTGASGDA
                     ARGRALVVDRRKGLCLLCHSGPFPDVPFQGDLAPSLAGVGTRFDTAALRLRLVDSARI
                     TPDSLMPAYYRRAGLARVAPAYAGATLLSAADIEDVVAFLATLRD"
     gene            1471..1914
                     /locus_tag="PLQ11_09690"
     CDS             1471..1914
                     /locus_tag="PLQ11_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018263077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxY-related AACIE arm protein"
                     /protein_id="HRK25211.1"
                     /translation="MTFDLNRREMLGTMLLTSGLGPARAASGEMEAAILAFTGGVVPQ
                     PGKVTLDIAPLVENGNSVAVSVGVDHPMRHDAHVTTIALFTEKNPQPNVATFHLSPRS
                     GRAFVATRMRLATSQHVAAVARLSDGSLWSDIREVIVTIAACTEE"
     gene            1919..2242
                     /gene="soxZ"
                     /locus_tag="PLQ11_09695"
     CDS             1919..2242
                     /gene="soxZ"
                     /locus_tag="PLQ11_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HRK25212.1"
                     /translation="MANALIDRPDNARRGDIVTIRLVLSHVMETGYRRDISGKVIPRN
                     IIRNFVCRYDGAEIVRAEFTQAIAANPFLAFTTVATVTGPVTMTWDDDEGQRYSAETM
                     LNVRD"
     gene            2535..3287
                     /gene="soxA"
                     /locus_tag="PLQ11_09700"
     CDS             2535..3287
                     /gene="soxA"
                     /locus_tag="PLQ11_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016669 - oxidoreductase activity, acting
                     on a sulfur group of donors, cytochrome as acceptor
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019417 - sulfur oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxA"
                     /protein_id="HRK25213.1"
                     /translation="MAITLLGVPVLAQGIPSAERQSGSAVLAPDTRAMQDNDRINPAM
                     LWAMEGERLWSEPAGPEGRSCRACHGPAEQAMHGVAVRHPRFDPASGQPVDLDGQIES
                     CRTGRQQAPAFGRESRSLLALSAYLGRQSRGLSIAAPEDRRLEPALALGEALFKARIG
                     QLNLSCAQCHDALWGRRLGGSLIPQGHPDGYPVYRLEWQSLGSLQRRIRNCMTGVRAE
                     PYPYGSAEMVALELYLKRRAAGMPLEAPGVRP"
     gene            complement(3230..3973)
                     /gene="phoB"
                     /locus_tag="PLQ11_09705"
     CDS             complement(3230..3973)
                     /gene="phoB"
                     /locus_tag="PLQ11_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HRK25214.1"
                     /translation="MTSTRILIVEDEADLALLISYNLKAEGFAVEAIGDGAEAALRLS
                     ERLPDLLLLDWMLPGLSGIELCRRLRQAPETRTLPIIMLTARGEEPDRVRGLETGADD
                     YLVKPFSMPELMARIRSLLRRSHPERIADRLIAGDIELDRQTRRVSRAGEAILFGPTE
                     FQLLDVLMSRPGRVFDRQRLITEVWGEDAEIEDRTIDVHIGRLRRALNRGDEADPLRT
                     VRGVGYSFDETYGRTPGASSGMPAARRLR"
     gene            complement(3970..5211)
                     /locus_tag="PLQ11_09710"
     CDS             complement(3970..5211)
                     /locus_tag="PLQ11_09710"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRK25215.1"
                     /translation="MKHRTLAALLAIALLTGLTAWALPWGSFWLLATGLVVGAGLARL
                     AAVRSGGTGATRKTEVAPLDPLATLADFAETLTLPVLAIDSRLTVRIANRASRQAFPA
                     LRSGEPMSFALRDPALVAGVQASLADGAERSVDLHERVPVERAFACALARFTLTGKGT
                     QPQTLVSVLVQDRTQQRAVEAMRVDFIANASHELRTPLTAILGSAETLQGPARNDPAA
                     RDRFLGLIAEQGRRMARLVDDLLSLSRIELKERQAPQGHVDLAAIAREAANTLSGLAT
                     DRDVTVEVLPPTTPTLVIGERDELLSVLDNLIENAIKYGHSGKRVEIAFGGGKGKDVT
                     VSVRDFGPGIAAEHLPRLTERFYRIDNALSRAEGGTGLGLALVKHTVNRHRGRLSIQS
                     TPGAGATFTISLPCAPASEGP"
     gene            complement(5208..6173)
                     /locus_tag="PLQ11_09715"
     CDS             complement(5208..6173)
                     /locus_tag="PLQ11_09715"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HRK25216.1"
                     /translation="MLRRKRLFQRVGTVASFVLFVTAIGVLGYIIRDLDVSKVRAAYD
                     QTSWWQIAMALGLSGLSYFLLTGYDALGLREVVDQAVPYGLTALASFTSFAVSFTLGF
                     PLLTAATVRFWVYSSIGLGAGAIASLTLIAGLTFWLGMSLVLGGALVTQPTTAGAFTR
                     LSEMTNFYIGATILGAIGFYLLWIAFRPRALTVQGWTFRLPRLPVTFAQVLLGAVDVC
                     VSCGVLFVLLPDGHGIAYATVIAAYVFANLLGIASHAPGGIGVFEATILLALPGIGRE
                     ELLGSVLLYRCCYYLLPFILAIWLLGLREIVLRARKLRTEIGEPS"
BASE COUNT         1090 a         2042 c         2074 g         1090 t
ORIGIN      
        1 cgctgtgatg tctacaagct gcatctcggc ggcggcttcg gccggcgcgg acgcgccgat
       61 tacgtcactc aggcggtcaa catcgcccgg cagatgcccg gcgtcccgat caagctgctg
      121 tggagccgcg aggaggacat gctccacggc acctatcatc cgatcaccca gtgcaagctg
      181 gtgggagggc tcgacaggga cggcaatctt gtcggcctgc acatgcgcct gtcgggccag
      241 tcgatcctgg ccggtgtcaa tccgcagggg ttgcagaacg gccgtgatcc gatcgccttc
      301 caggggctca atgcgggtgg gccggagggg caccttggct atgccatccc caatctgctg
      361 atcgatcatg cgatccgcaa tccgcacgtc ccgcccggct tctggcgcgg cgtcaatctc
      421 aaccacaacg cgatctatct ggagtgtttc atcgacgaac tggcgcatgc ggcaggggtc
      481 gatccgctgg ccttccggca gaagatgatg ggcccctatc ccaaacaccg cgccgtgctc
      541 aacgcggtgg cggaggggat tggctggagc aagccggcgc cggccggcgt ctcacgcggc
      601 cttgcgcaga tcatgggctt tggcagctat gtcgccgccg ccgccgaagt ctcggtggag
      661 gccagtggca gcatcagggt gcaccggatc gtcgccgcca ccaatcccgg ccatgtggtc
      721 aatccccagc aggtggccgc gcaggtggaa ggctcgttcg tctatggcct gtcggcggcg
      781 ctgatgcagc aatgcacggt gaagaacggc cgtatcgtcg aggagaattt cgacacctat
      841 ccggccctgc ggattgccga gatgccgaag gtggaaagcc tgctgcttcc ctcgggcgat
      901 ttctggggtg gagtgggcga gccgaccatc gccgttgcag cgccggccgt gctcaatgcg
      961 gtctttgctg ccaccggcaa gcgcgtccgc agcctgccga tccgcaccgc tgaactgaaa
     1021 cgtgcgtgat gcggcccctg tttctggccg gatgtcttgc gctggccgcg ctgccggtcg
     1081 gcgctgtcga accggaggcc atcgtcgtgc gcgaaggtgg catccccgcc tcgctgaccg
     1141 gcgcgtccgg cgatgctgcc cgtggccgcg cgctggttgt cgatcggcgc aagggtctgt
     1201 gtctgctgtg tcattcagga ccgtttccgg acgtgccttt tcagggcgat ctcgcccctt
     1261 cgctggccgg tgtcggaacg cggttcgata ccgccgcctt gcgcctcagg ctggtcgaca
     1321 gcgcgcgcat cacgcccgat tcgctgatgc ctgcctatta ccgccgagcg ggtctggcgc
     1381 gcgtggcgcc cgcctatgcc ggggcgacgc tgctgagcgc cgccgacatc gaggatgtgg
     1441 tcgcctttct ggccaccttg agggactgac atgaccttcg atctgaaccg ccgcgagatg
     1501 cttggcacaa tgctgctgac gtccggcctt ggtccggcgc gcgccgcgag cggagagatg
     1561 gaggcggcaa tcctcgcctt taccggcggc gtggttcccc agcccggcaa ggtgacgctc
     1621 gacattgccc cgctggtcga gaacggcaat tccgttgcgg tctcggtggg tgtcgatcac
     1681 ccgatgcggc atgatgccca tgtgacgacg attgcgctct tcaccgagaa gaatccccag
     1741 ccgaatgtcg ccaccttcca tctgtcgccg cgttccggcc gtgccttcgt cgcaacgcgc
     1801 atgcgcctgg cgacctccca gcatgtggcg gcggtcgccc ggctcagcga cggcagcctg
     1861 tggagtgaca tccgtgaggt catcgtcacc atcgccgcct gcaccgagga atgacgtcat
     1921 ggccaacgca ctcatcgacc ggccggacaa tgcgcgacgc ggtgatattg tcaccatccg
     1981 gctggtcttg tcgcatgtga tggagaccgg gtatcggcgc gatatttccg gcaaggtgat
     2041 cccgcgcaac atcatccgca attttgtctg ccgctatgac ggcgcggaga tcgtccgggc
     2101 ggaattcacg caagccattg ccgccaaccc cttcctggcg ttcacaacgg tcgcaaccgt
     2161 caccggaccg gtgacgatga cctgggatga cgacgagggc cagcgctaca gcgccgagac
     2221 gatgctgaat gtccgggact agtgcatctt ccgaccagat tgaatcgttg cgatggccag
     2281 tttcaggtca agggccagga aaatgcgaga agcgtatcct gcgatccggt tcgagcattt
     2341 tccgccggat ctggcccgaa aatggccatc ccttcgggtt tccgggccgc gggcgcccgg
     2401 tttggacgct ggctcgaccg tatcgacaga tacatccttc gccagcctcc gcccggatca
     2461 tcccacggcc cgagaaacgc aactggttca atctggtcgg aagatgctct cggttttgcc
     2521 aatgcctggc ctggttggcg atcacgctgc tgggggtgcc cgtgctggcg cagggcatcc
     2581 cgtccgccga gcgtcagtcc ggctcggcgg tgctcgcccc cgatacgcgc gcgatgcagg
     2641 acaacgaccg catcaatccc gccatgctgt gggcgatgga aggcgagcgg ctctggtccg
     2701 aacccgcagg gccggagggt cggagctgcc gcgcctgtca tgggccggcc gaacaggcga
     2761 tgcacggtgt cgccgtccgc catccaaggt ttgatccggc cagcggccaa cctgtcgatc
     2821 tcgatgggca gatcgaaagc tgtcgtaccg gtcgccagca ggcgccggca ttcggtcggg
     2881 aaagccgcag ccttctggcg ctctccgcct atctcgggcg ccagtcacgc gggctgtcga
     2941 ttgcggcccc ggaggatcgg cggcttgagc cagccctggc gctgggcgaa gcattgttca
     3001 aagcccgcat cggccagctg aacctgtcct gcgcccaatg tcacgatgcg ctttgggggc
     3061 ggcggctcgg tggttcgctc attccgcagg ggcatccgga tggttatccg gtctaccggc
     3121 tggaatggca gagcctgggc tcgctccagc gccgcattcg taattgcatg accggtgtgc
     3181 gggctgagcc ttatccctac ggttcggcgg agatggtggc gctggagctt tatctcaagc
     3241 ggcgggcagc cggcatgcca ctcgaagcgc cgggagtgcg tccataggtt tcgtcgaagg
     3301 aatagccgac accgcgcacg gtgcgcagcg gatcggcctc atcgccccgg ttcagcgccc
     3361 gccgcagccg cccgatatgg acatcgatgg tgcgatcctc gatttcggca tcctcgcccc
     3421 agacttcggt aatcagcctt tgccggtcga acacccgacc gggtcggctc atcagcacat
     3481 cgagcagttg aaactcggta gggccaaaca ggattgcctc gccggcgcgg ctgacgcgcc
     3541 gcgtctggcg atccagctcg atgtccccgg cgatcaggcg atcggcgatc cgttcggggt
     3601 gactgcggcg gagcaaagag cggatgcggg ccataagttc aggcattgag aagggcttga
     3661 ccagataatc gtcggcgccg gtctcaagtc cgcgtacgcg atccggctct tccccgcgcg
     3721 cggtcagcat gatgatcggc agggtccggg tctccggcgc ctgacgaagc cggcggcaga
     3781 gttcaatccc tgacaggccc ggcagcatcc agtcgagcag cagaagatcc ggcagtcgtt
     3841 cgctgaggcg cagggccgct tcggcaccat cgccgatcgc ctcgacggca aaaccctcag
     3901 ccttgaggtt gtaactgatc agaagcgcga gatccgcctc gtcctcgacg atcagaattc
     3961 gggtactggt catggaccct ccgatgcggg agcgcatggc aacgatatgg tgaaggttgc
     4021 cccggccccc ggtgtgcttt gaattgaaag ccggccgcgg tgccggttga cggtatgctt
     4081 gaccagagcc aggccgagcc cggtgccgcc ttctgcccgg ctcaaggcgt tgtcgatgcg
     4141 gtagaaccgt tcggtcagcc ttggcagatg ttcggcagca attccggggc cgaaatcacg
     4201 cacgctgacc gtgacatcct tgcccttccc gccgccaaag gcgatctcga ccctcttgcc
     4261 ggaatggcca tacttgatcg cgttctcgat caggttgtcc aggaccgaca gaagctcgtc
     4321 gcgctcgccg atcaccaggg tcggtgtcgt cggcggcagg acctcgaccg tgacgtccct
     4381 gtcggtagcc agaccggaga gggtgttggc ggcttcgcgg gcgatggcgg caagatcgac
     4441 gtggccctgt ggcgcctgtc gctccttgag ctcgatccgt gacagcgaca ggaggtcgtc
     4501 gacaagccgc gccatccgcc gtccctgttc ggcaatcagg ccgagaaacc gatcgcgcgc
     4561 cgccggatca ttgcgagcag ggccctgcag ggtctcggcc gagcccagga tagcggtcag
     4621 cggtgtgcgc agttcgtggc tggcattggc gatgaaatcc acccgcatcg cctcgacagc
     4681 ccgctgttgc gtccggtcct gcacaagcac gctgaccaag gtctggggct gcgttccttt
     4741 ccctgtgagg gtgaagcgtg ccagagcgca ggcgaaggcg cgctccactg gcacgcgttc
     4801 atggagatcc accgatcttt cggcgccatc ggcgaggctg gcctgcaccc cggctaccag
     4861 cgccggatcg cgcaaagcaa aagacatggg ctcgccgctg cgcagggccg gaaacgcctg
     4921 ccggctcgcc ctgttggcga tccgcaccgt caggcgcgag tcgatggcca gcaccggcag
     4981 ggtcagcgtt tcggcaaaat ccgccagcgt cgccagtgga tcgagcgggg caacctcggt
     5041 cttgcgggtc gcgccggtcc cgccactccg cacagcagcg agccttgcca gcccggcgcc
     5101 cacgacaagg ccggtggcga gcaaccagaa gctgccccag ggcagagccc aggccgtcag
     5161 tcccgtcagc aatgctatgg cgagaagtgc ggccagcgtg cgatgcttca cgaaggctcc
     5221 ccgatctcgg tcctcagctt tctggcccgc aggacgatct cgcgcaggcc cagcagccag
     5281 atggccagga tgaatggcag caaatagtag cagcaccggt aaagcagcac cgagccgagc
     5341 agttcctcgc ggccgatgcc tggcagggcc agcaggatgg tcgcctcgaa aaccccgatt
     5401 ccaccgggag cgtgactggc gatgccgagc aggttggcaa agacataggc cgcgatgacg
     5461 gtcgcatagg caatgccatg gccgtccggc agcagcacga acagcacgcc gcaggacacg
     5521 cagacatcga ccgccccgag caggacctgc gcgaaagtca ccggcaggcg tggcagccgg
     5581 aaggtccagc cttgcaccgt cagcgcccgg gggcggaagg cgatccacag cagatagaag
     5641 ccgattgctc cgagaattgt cgcgccaata tagaaattgg tcatttcgga aaggcgcgtg
     5701 aaggcgccag cggtggtcgg ctgcgtgacc agcgctccgc ccagcacaag cgacatgccg
     5761 agccagaagg tcaggccggc gatcagcgtc aggctggcga tcgcccccgc gccaagaccg
     5821 atcgacgaat agacccagaa ccggacagtc gccgccgtga gtagtggaaa gcccagcgtg
     5881 aaactgacgg caaagctggt aaagctggcc agcgccgtca gaccgtaggg cactgcctga
     5941 tcgacgactt cgcgcaggcc gagcgcgtca tagccggtca gcaggaaata actcaggccc
     6001 gaaaggccga gtgccatggc gatctgccac cagctggtct ggtcataggc agcccgcacc
     6061 ttgctgacgt cgaggtcacg gatgatatag ccaagcacgc cgatggcggt cacgaacagc
     6121 acgaatgagg cgacggtgcc gacacgctgg aacaggcgct tgcgcctgag cacgcgcgct
     6181 ttgcgttccc aatggccggt ctcgttacga ggggagcctt gttcgggcgc gccgctgtcc
     6241 attagagcac tttccgacca gattggatca gttgcgtttc tcgggccgtg cgggat
//
LOCUS       DAOMXG010000144         6205 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_6680_length_6205_cov_9.714472, whole genome shotgun sequence.
ACCESSION   DAOMXG010000144 DAOMXG010000000
VERSION     DAOMXG010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 6205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6205
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6680_length_6205_cov_9.714472"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PLQ11_09720"
     CDS             <1..200
                     /locus_tag="PLQ11_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25217.1"
                     /translation="KKATDKQATDKKATDKQATDKQATDKKATDKKATDKKATDKQAT
                     DKKATDKQATDKKATDKPTKG"
     gene            279..2006
                     /locus_tag="PLQ11_09725"
     CDS             279..2006
                     /locus_tag="PLQ11_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25218.1"
                     /translation="MSAGCAASLGLAEVEALRRLARQFEAGSDAAKLAELERCASLPI
                     LAPEVLVAWHDVLLFMLAYPQTPALRARCESELSRCAELARVLAGTGEDLPDALHGSG
                     LAWTRVAVTFNHQVARWLADTFPGVAGFHAARGSADTLRKLLRESLPDAEFEALAEPA
                     EDAGALIENLGALAALAAADPLRWLLDNLDASGLPDRMLRSLYDDLDVFVGLRSQDSL
                     LTRARLRGLDRPIAYQDRPLARSEDARALLDEPLAPAIDLTDDDRARLLTTARGLLAL
                     LGRQTEPISSCNGTGVMAYDLGRGVVIALFSTAPADRTPLDSHVGMMLFKNGLPVAYG
                     GGWPFLGVCKIGINIFEPYRGGESHFLFLSVMRAYRHLFGIRRFIVERYQFGRNNPEG
                     IASAAYWFYYRLGFRSMRTDLASLAEREWARLRDGKVKATTRRTLERFTEAHMEMRLP
                     GCPDSFDYCDPADLSRTVTAVVKRRFAGDRKAFSAWAETQLRAILDFPAGLSPQQRRA
                     LALLAPVVALIPGLAQWSAGERTQVRDWILSRTGEDERPYFALLTGHPRLQAALEALP
                     QLDDGEALS"
     gene            complement(2074..3258)
                     /locus_tag="PLQ11_09730"
     CDS             complement(2074..3258)
                     /locus_tag="PLQ11_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HRK25219.1"
                     /translation="MPAKGPKPLAGLRIVEFSHMVMGPTSGLIFGDLGADVIKVEPMG
                     EGDNTRRLAGSGAGFFSTFNRNKRSIQVDLKSQQGLAFIRRLIATADALTENFRAGAL
                     EAMGLGYEVLARDNPRLIYCSLKGFLSGPYETRAALDEVVQMMGGLAYMTGPSGRPLR
                     AGSSVNDIMGGMFAVIGILAALQERARTGRGQFIKAGLFESNMLLVAQHMMQFAVTGT
                     PARPMPERIAAWAVYDVFDTSDGEQVFVGVVSDTQWAVFCDAFGLADLKGHPDLGSNR
                     DRVLKRDQFMPRLKALFAGLDRATILQKCEAIRLPFAPIMRPQDLFDDPHLNHPGAMI
                     DITLMDGRSARVPALPLELSGERLGLHQDLPGPGEHSVAVARELGYDEADIGRLRAAG
                     VI"
     gene            complement(3264..4211)
                     /locus_tag="PLQ11_09735"
     CDS             complement(3264..4211)
                     /locus_tag="PLQ11_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HRK25220.1"
                     /translation="MTGKGEGGRDVLISECGPRDGLQILKQIMATEDKCRWIAALHAA
                     GLREIEVCSFVPARLMPQMADAADVVGFARTLPGLTVLALVPNLRGAEAALKAGATKL
                     TVPVSGSEGHSRANVRRSRSEMVDEVAAIVRLRDALAPDVPVEANVSVAFGCTIDGHV
                     DEDEVIALAERLVAVGVDDSGLSDTTGMGNPAQVRRMFSRLIARIGDRCGAAHMHNTR
                     GLGIANTLAAYEAGVRIFDSSHCGLGGCPHAPGASGNVVTEDLVFMFEAMGIRTGIDP
                     AALVAARALVAEALPGVELYGMLAGAGLPKNFPYPHPGS"
     gene            4313..5026
                     /locus_tag="PLQ11_09740"
     CDS             4313..5026
                     /locus_tag="PLQ11_09740"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRK25221.1"
                     /translation="MPRKAAHMPRADQNRSEGGASTVDRTLALLACFTIDRPTPSLIE
                     LAAETGLYKSTVLRLLASLRHARIVRQQAGGRYALGPEVARLNQVFQRSFAEAEAILP
                     HLRTLVEKTNESAAFYVPQGDRRLCLYRIDCSRPLRDSLKVGDLLPLDRGAAGRVIQA
                     FQGAVDDRAAAIRAAGAVALRGDRVPELSAIAAPAFTASGDLLGAVTLIIPSERFTEA
                     FVADVVATGKAITRDLGGA"
     gene            5254..>6205
                     /locus_tag="PLQ11_09745"
     CDS             5254..>6205
                     /locus_tag="PLQ11_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid TRAP transporter substrate-binding
                     protein SiaP"
                     /protein_id="HRK25222.1"
                     /translation="MTHHPNRRAVLSGLAGLAAAGSSAFPAAAQQKVLLRLSTPATPS
                     DQRAIGLIEKFGPAVAGFAEFQPHWNATLFKQGTELEAIARGNLEMSIASAQELSTIF
                     PQWSIFTAGYLLRDAEHQKKVFASDVMKPMKQMVEDKLGVKLISVMYLGKRHVNLRSD
                     KEIRTPADLNGVKLRMPGAESWLFLGKALGANPLPMAFTEIYTALQTGAIDGQDNPLP
                     TVRDSKFYEVTKQIALTAHLVDWNFLAFSKKVWDGMKPEQQAIVQKAADDACEAARQA
                     QLKLEAELEAFMKEKGLKVYTPDVDAFRAHVQKEYLASKFA"
BASE COUNT         1094 a         2100 c         1988 g         1023 t
ORIGIN      
        1 acaagaaggc cacggacaag caggccaccg acaagaaggc cacggacaag caggccaccg
       61 acaagcaggc cacggacaag aaggccaccg acaagaaggc caccgacaag aaggccacgg
      121 acaagcaggc caccgacaag aaggccacgg acaagcaggc taccgacaag aaggctaccg
      181 acaagccgac gaagggctga cctgacagaa cctgtctgcg gattggtgcc cggtgcgccg
      241 tctcaggcgc tccggcgccc ctgtttctgg aggggaagat gagtgctggc tgtgccgcga
      301 gcctcggctt ggcggaggtg gaggcgttgc gccggctggc gcgccagttc gaggcgggat
      361 ccgatgcggc caagctggcc gaactggagc gctgcgcaag cctgccgatc cttgcgcccg
      421 aggtgctggt cgcctggcat gacgtgctgc tgttcatgct cgcctatccc cagacgccgg
      481 cattgcgcgc ccgttgcgag agcgagttga gccgctgtgc cgagctggca cgcgtgctgg
      541 ccggcaccgg cgaggacttg cccgatgcgc tgcatggctc gggtctggcg tggacacggg
      601 ttgccgtcac gttcaatcat caggttgcac gctggctggc cgacaccttc ccgggcgtcg
      661 ccggctttca cgccgcacgc ggcagcgccg acacattgag aaaactgctc agggagagcc
      721 tgccggacgc cgagttcgaa gcgctcgccg aaccggcgga ggatgccggc gcgctcatcg
      781 aaaacctcgg cgccctcgcc gccctcgccg ccgccgatcc cttgcgctgg ctgctggaca
      841 atctcgatgc atccggcctg cccgaccgga tgttgcggag cctttatgac gatcttgatg
      901 tgttcgtcgg cctgcgctca caggacagcc tgctgacccg cgcccggctg cgcggccttg
      961 accggcccat tgcctatcag gaccgcccgc tcgcccgctc ggaggacgcc cgggcgctgc
     1021 tggacgaacc gctggcgccc gccatcgacc ttaccgacga tgaccgggcc cgtctgctga
     1081 ccaccgcgcg cggattgctg gcgttgctgg ggcgccagac cgagccgatc tcatcctgca
     1141 atggaacggg cgtgatggcc tatgatctcg gccgcggggt ggtgatcgcg ctgttttcca
     1201 ccgccccggc cgaccgcacc cctctggatt cgcatgtcgg gatgatgctg ttcaagaacg
     1261 gcctgccggt ggcctatggc ggcggctggc cgttcctcgg tgtctgcaag atcggcatca
     1321 acatcttcga gccctatcgc ggcggggaat cgcatttcct gttcctgtcg gtgatgcgcg
     1381 cctatcgcca cctgttcggc attcgccgct tcatcgtcga gcgctaccag tttggccgca
     1441 acaaccccga aggcatcgcg tcagccgcct actggttcta ctaccggctg ggctttcgct
     1501 ccatgcgcac cgatcttgcc agtctggccg aacgcgaatg ggccaggctg cgcgatggca
     1561 aggtcaaggc caccacccgc cggacgctcg aacgtttcac cgaggcgcat atggaaatgc
     1621 gcctgcccgg ctgtcccgac agcttcgact actgcgatcc ggccgacctg tcgcgcacgg
     1681 tgacggcggt ggtcaaacga cgcttcgccg gcgaccggaa ggcgttttct gcctgggccg
     1741 agacccagct gcgggccatt ctcgactttc ccgcagggct gagcccccag caacgccgtg
     1801 ccctggctct gctggcgccg gtggtcgcgc tgatccccgg tctggcgcaa tggtccgccg
     1861 gcgaacgaac ccaggttcgt gactggattc tgtcgcgcac cggtgaggat gaacggccgt
     1921 atttcgccct gctgaccggc catccgcgcc tccaggcggc gcttgaggcg ctgccgcaac
     1981 tggacgatgg cgaagcgctg tcctgagaaa cactcccgcg ccgtcagagc ggatgggtcg
     2041 aaccggaagc cacttcgctt gaaaatgctc tgatcagatg acgccggcgg cgcgcaggcg
     2101 tccgatatcc gcctcgtcat agccaagctc acgcgccacc gcgacggaat gttcgcccgg
     2161 tccgggcagg tcctggtgca accccagccg ctcgccggag agttccagcg gcagcgccgg
     2221 cacacgcgcg ctgcgtccat ccatcagggt gatgtcgatc atcgcgcccg ggtgattcag
     2281 atgcggatcg tcgaacaggt cctgcggccg catgattggc gcgaacggca agcggatcgc
     2341 ctcgcatttc tgcaggatcg tcgcccggtc gagaccggca aacagcgcct tgagccgggg
     2401 catgaactgg tcccgcttca gcaccctgtc gcggttgctg ccgagatcgg ggtggccttt
     2461 cagatccgca agcccgaagg cgtcgcagaa caccgcccat tgcgtgtcgg agacaacgcc
     2521 gacgaacacc tgttcgccgt cggacgtatc gaacacgtca tagaccgccc aggcggcgat
     2581 gcgctctggc atggggcgcg cgggcgttcc ggtcacggca aactgcatca tgtgctgggc
     2641 gaccagcagc atgttgctct cgaacagccc cgccttgatg aactggccgc ggccggtgcg
     2701 ggcccgctcc tgcagggcgg cgaggatgcc gatgacggcg aacatgccgc ccatgatgtc
     2761 gttgaccgat gatccggcgc gcaaggggcg gccggagggg cccgtcatat aggcaagccc
     2821 gcccatcatc tgcaccacct cgtcaagcgc ggcgcgcgtc tcgtagggcc cggacaggaa
     2881 gcctttcagc gaacaataga tgaggcgcgg attgtcccgg gccaggacct catagccaag
     2941 gcccatcgct tcgagcgcac cggcgcggaa attctcggtc agcgcatcgg ccgttgcgat
     3001 cagccggcgg atgaaggcaa ggccctgctg cgacttgaga tcgacctgga tcgagcgctt
     3061 gttgcggttg aaggtcgaga agaagccggc gcccgaaccg gcgaggcgcc gcgtgttgtc
     3121 gccctcgccc atcggctcga ccttgatgac atcggcgccg agatcgccga agatgagacc
     3181 ggaggtcggt cccatcacca tatgggagaa ttcgacgatg cggaggccgg cgagcggctt
     3241 cgggcccttg gcaggcatgg cgatcagctc ccgggatgcg gatagggaaa gttcttcggc
     3301 aggcccgcac cagcgagcat tccgtagagt tcgacgccgg gcagcgcctc agccaccagg
     3361 gcccgcgccg caaccagcgc cgccgggtca atcccggtgc ggatacccat cgcctcgaac
     3421 atgaacacca gatcctcggt caccacattg cccgaggcgc ccggcgcatg tggacagcca
     3481 cccagaccgc aatgcgatga atcgaagatg cgaacgccgg cttcataggc cgccagcgtg
     3541 ttggcaatgc caagcccgcg cgtgttgtgc atatgcgctg caccacatct gtcaccgatg
     3601 cgcgcgatca accgagaaaa catgcgccgc acctgcgccg gattgcccat accggtggtg
     3661 tcggacaggc ctgaatcgtc aacgccaacg gcgacaagcc gttcagccag cgcgatcacc
     3721 tcatcctcat cgacatgccc gtcaatggtg cagccaaagg ccaccgagac attggcctcc
     3781 accggcacat ccggagccag agcatcgcgc aggcggacga tggcggccac ctcgtcgacc
     3841 atctcgctgc ggctccggcg cacattggcg cgcgaatgcc cctcggaacc ggagaccggg
     3901 accgtcagct tggttgcccc ggctttcagc gccgcttcag ccccgcgcag gttcggcacc
     3961 agcgccagaa ccgtaaggcc gggcagcgtg cgggcaaagc cgaccacatc ggccgcatcg
     4021 gccatctgcg gcatcaaccg ggccggaaca aacgagcaga cctcgatttc cctgaggccg
     4081 gccgcatgca acgccgctat ccaccggcat ttatcctcgg tcgccatgat ctgtttgagg
     4141 atctgcagtc cgtcacgcgg accgcattcg cttatcagca catcgcgccc gccctcgcct
     4201 ttgccggtca tgtccgtcgc gctgcctctg attgacagga atcgttctat cttttagaat
     4261 gaaattctat tttattgctg atgcgccaat tagtcaattc gggggggatg acgtgccgcg
     4321 caaagccgcc catatgcccc gcgccgacca gaaccgctcg gagggcggcg cttcgaccgt
     4381 cgatcggaca ctggctctgc tggcctgttt caccatcgac cggccgacgc cgagcctgat
     4441 cgagcttgcg gcggaaaccg gcctctacaa gagcacggtg ctgcggctgc tagcgtcgct
     4501 gcgtcatgcg cggatcgtcc ggcagcaggc gggcggtcgt tatgcgctgg ggccggaagt
     4561 ggcgcggctg aaccaggtct ttcagcgctc ctttgccgag gccgaggcga ttctgccgca
     4621 tcttcgaacg ctggtcgaaa agaccaacga gagcgctgcc ttctatgtcc cgcagggcga
     4681 caggcggctc tgcctctacc ggatcgattg cagccggccc ttgcgcgaca gcctcaaggt
     4741 cggcgatctc ctgccgctcg accgtggcgc cgccgggcgg gtaatccagg cgttccaggg
     4801 cgctgtggac gaccgggccg cagccatccg tgccgcaggg gcggtcgcgc tcaggggcga
     4861 tcgggtgccg gagctttccg ccatcgccgc gccggctttc acggcttcgg gggatctgct
     4921 cggggcggtg acgctgatca ttcccagcga gcggttcacc gaggcgtttg tcgccgatgt
     4981 cgtcgcgacc ggcaaggcga tcacgcgaga cctcggcggc gcctgagcct ggatgagccc
     5041 ggcatcaggc cacttccccg ggccgcccgc gccagcccgc cccggaaatt gtcgagaaac
     5101 ccgcgcgtct gctgattttt cagtggaact ggcgttgcct gacgtcctac aaatggcaaa
     5161 ttctgtccgg actggaccgc agggcgccct caggctggca tgcgggatag caggcaggct
     5221 caacgagacg acaggattca caggaggacc tcaatgacgc atcatcccaa ccgccgcgct
     5281 gttctgtccg gtctcgccgg gcttgcggct gccggctcgt ccgcttttcc ggccgcggcc
     5341 cagcagaagg tgctgctgcg cctgtccacg cctgcgaccc cgtcggatca gcgcgccatc
     5401 ggcctgatcg agaagttcgg cccggcggtc gccggcttcg ccgaattcca gccgcactgg
     5461 aacgccaccc tgttcaagca gggtaccgag cttgaggcca ttgcccgcgg caacctcgaa
     5521 atgtcgatcg cctcggcgca ggaactctcc accatcttcc cgcaatggtc gatcttcacc
     5581 gccggctatc tgctgcgcga cgccgagcac cagaagaagg tgttcgcctc cgacgtgatg
     5641 aagccgatga agcagatggt cgaggacaag ctcggcgtga agctgatctc ggtgatgtat
     5701 ctcggcaagc gccacgtcaa cctgcgctcc gacaaggaaa tccgcacccc ggccgatctc
     5761 aacggcgtca agctgcgcat gccgggggcc gaatcctggc tgttcctcgg caaggcgctg
     5821 ggggccaatc cgctgccgat ggcgttcacg gaaatctaca ccgcgttgca gaccggcgcc
     5881 atcgacgggc aggacaatcc gctgccgacc gtgcgcgatt ccaagttcta cgaggtgacc
     5941 aagcagatcg cgctgaccgc ccatctggtc gactggaact tcctcgcctt ctccaagaag
     6001 gtgtgggacg ggatgaagcc ggagcagcag gccatcgtgc agaaggccgc cgacgacgcc
     6061 tgcgaggcgg cccggcaggc ccagctcaag ctggaagcgg aactcgaggc cttcatgaag
     6121 gaaaaggggc tgaaggtcta cacaccggat gtggatgcct tccgcgccca tgtgcagaag
     6181 gaatacctcg cctccaaatt cgcca
//
LOCUS       DAOMXG010000145         6166 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_6742_length_6166_cov_8.591065, whole genome shotgun sequence.
ACCESSION   DAOMXG010000145 DAOMXG010000000
VERSION     DAOMXG010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 6166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6166
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6742_length_6166_cov_8.591065"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1284)
                     /locus_tag="PLQ11_09750"
     CDS             complement(<1..1284)
                     /locus_tag="PLQ11_09750"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRK25223.1"
                     /translation="MTFSELGLSERVLAAVESTGYTTPTPIQAQAIPEVLRRRDVLGI
                     AQTGTGKTAAFTLPMLSLLEQGRARARMPRTLILEPTRELAAQVEASFLRYSDNQKLN
                     VALLIGGVSFGDQDAKITRGVDVLIATPGRLLDHFERGKLLLTGIEILVIDEADRMLD
                     MGFIPDIERICKLVPFTRQTLFFTATMPPEIQRITEQFLSNPARIEVARASSTASTIT
                     QRLVAAGSKDFQKREVLRDLIRSQESFKNAIIFANRKVEVATLCRSLVRHGFNAAAIH
                     GDLDQPARMKALDGFRNGDVTLLIASDVAARGLDIPDVSHVFNYDLPSHAEDYVHRIG
                     RTGRAGKSGTAISLVTSRDDKYLAAIEGLIKTKIEWQGPTLAEYVPPPFEERPSSRRE
                     RSPRKPLSTRPRRDYAPKRESSEPLASDAAMEPLRP"
     gene            complement(1508..1798)
                     /locus_tag="PLQ11_09755"
     CDS             complement(1508..1798)
                     /locus_tag="PLQ11_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25224.1"
                     /translation="MRHLSSLLGSGSMARDYAVVGILVAVLAVFSVQQFNTFMGSMRQ
                     NVRVQSATLRPQEPLPRNYAINRSVLDDTVTTGSISGRPVVLDPCTGQVKSK"
     gene            2489..3208
                     /locus_tag="PLQ11_09760"
     CDS             2489..3208
                     /locus_tag="PLQ11_09760"
                     /inference="COORDINATES: protein motif:HMM:NF019430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HRK25225.1"
                     /translation="MSGMTDFIRRRPIISTLVGVAVVIGGVGAVSAQYYGGPGSWGRG
                     GSGGWGRGGIERFCTVPHARFQPVMRAYIKADLNMTGAQAAEFDKLADVLAPAFEEIK
                     ADLCGSFRADSPKITPPEKLEKIAAALRKAATAAENAVAPARSLYGQLDDAQKAEIDR
                     RMERRRMAREGRGGYGEGRGGYGEGRGMGRDRDEHRGWHEHGMGPRRDGQGGWERGPG
                     QPGFGQSPEGPGGPFFRGGQQ"
     gene            complement(3272..3721)
                     /locus_tag="PLQ11_09765"
     CDS             complement(3272..3721)
                     /locus_tag="PLQ11_09765"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003602126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HRK25226.1"
                     /translation="MSEILFYHLQSRPLDQSLPLLIEKSRARGWKVVLRAGSDERLQA
                     LDDLLWTFADDSFIAHGTAADGDPASQPVLLTTKDERPNQAEVLFLVDHAPLPADYPY
                     ERVVVMFDGNDPEALDRARADWKAVKALGHAATYWQQDEAGRWIKRS"
     gene            3774..4892
                     /locus_tag="PLQ11_09770"
     CDS             3774..4892
                     /locus_tag="PLQ11_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HRK25227.1"
                     /translation="MHDFDTAAILAHLRRWVEIESPSDVPSAVNAMVDALAADYARLP
                     VTQERVAGAPGFGDHLIVRSAWGQDRPGILLLSHSDTVHPLGFILDRLPFRIEGDRAY
                     GPGIYDMKGGLCIAYHAFAAIAAAGGSPLGITHLIVSDEEIGSPSSRPLIERLGKAAS
                     HVLVTEPARDGGKIVVGRKGTGDFRLTIHGRASHAGTKPEEGRSAVRELAHQILQLEA
                     LSDPASGVTVTVGVVRGGTRPNVVPDLAEADVDIRVPTLAAADHLIAAIRDRKPVTPD
                     VTIRVTGGLNRPPFEQSPAGRRLFDLAHAEAAKIGIDLVGVFTGGGSDGNFTAPTTPT
                     LDGLGVDGAGAHTDQEHALVSSMAPRADLLRRIMMTLR"
     gene            complement(4942..>6166)
                     /locus_tag="PLQ11_09775"
     CDS             complement(4942..>6166)
                     /locus_tag="PLQ11_09775"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HRK25228.1"
                     /translation="RDFVTLGGKVAGMLPAGRDVSILFDLAGAGADEAADFALGLGLR
                     SYRFDRYKTRKKADDDSGQPGAIAVHFADYSAARRAMRSRDGMLNGVTLARDLVNEPP
                     NVLTPVDFARRAGELAKLGVDIEILGEKDLKKIGMRALLGVGQGSEEDSQVVIMRWNG
                     AKDAKAQPIAFIGKGVVFDTGGISIKPAQGMEDMKGDMAGAACVVGLMHALAARKARC
                     NVIGAIGLVENMPDGKAQRPGDIVTSLSGQTIEIINTDAEGRLVLADVLWYVQERFKP
                     KFMVNLATLTGAILVALGQEHAGLFSNNDELAQRLYDCGKMTGEKVWRLPLGAEYDKM
                     IDSKFADMKNSGGRYGGSITAAQFLQRFVNDCPWAHLDIAGTGMGSPTTDINRSWGSG
                     WGVRLLDRLVSEYYE"
BASE COUNT         1090 a         2009 c         1926 g         1141 t
ORIGIN      
        1 cggccgcaag ggctccatcg ccgcgtcgct ggcgagcggt tcggacgatt cgcgcttcgg
       61 cgcatagtca cggcgcggcc gtgtcgaaag cggtttgcgc gggctgcgct cgcggcggct
      121 gctgggccgt tcctcgaacg gcggcggcac atattcggcg agtgtcggcc cctgccattc
      181 gatcttggtc ttgatcagac cttcgatggc cgccagatac ttgtcgtcgc ggctggtgac
      241 cagcgagatg gccgtgcccg acttgccggc gcggccggtc cggccgatgc gatgaacgta
      301 gtcctcggca tgactgggca ggtcatagtt gaacacgtgg ctgacatcgg gaatgtcgag
      361 gccgcgcgcc gccacatcgg acgcgatcag cagcgtcaca tcgccgttgc ggaatccatc
      421 cagcgccttc atgcgcgccg gctggtcgag atcgccatgg atcgcggcgg cattgaaccc
      481 atgccgcacc agcgagcggc acagcgtcgc cacctcgacc ttgcggttgg cgaagatgat
      541 ggcgttcttg aagctctcct gcgagcggat cagatcgcgc agcacctcgc gcttctggaa
      601 atcctttgac cccgcagcaa ccagccgctg ggtgatcgtc gaggcggttg acgaggcgcg
      661 cgccacctcg atccgtgccg gattggacag gaactgctcg gtgatgcgct ggatttccgg
      721 cggcatggtc gcggtgaaga acagcgtctg gcgggtaaac ggcaccagct tgcagatgcg
      781 ctcgatgtcc gggatgaacc ccatgtcgag catgcggtcg gcctcatcga tcaccagaat
      841 ctcgataccg gtcagcaaca gcttgccgcg ctcgaaatga tcgagcaggc ggccgggcgt
      901 ggcaatcagc acatcgacgc cgcgggtgat cttggcatcc tgatcgccga acgaaacccc
      961 gccgatcagc agtgccacat tgagcttctg attgtcggaa tagcgcagga agctggcttc
     1021 cacctgcgca gcaagctcgc gcgtcggctc aaggatcagg gtacgcggca tgcgcgcccg
     1081 cgccctgccc tgttccagca gcgacagcat cggcaacgtg aaggctgccg tcttgccggt
     1141 tccggtctgg gcgatgccca gcacgtcacg gcggcgcaac acttcgggaa tggcctgggc
     1201 ctgaatgggg gtcggcgtgg tgtagcccgt cgattccacg gccgcgagca cacgctcgga
     1261 caggccgagt tcagagaaag tcattggtcc gtctttccgg gagcgcccct gcgttccctt
     1321 tggtgcggac cggctctggc ccgccatctg tcgaatcatt cacctcgggc gaacatcacc
     1381 gcgaagttga ccgtctatgg ctcaaacggc cggaaattgc aacgatttgt cacagccgcc
     1441 aagaaaatgg gcgcctgccg gcgcccagag atacttggag gtcaatcaac tttttgacgc
     1501 gatagaacta cttggactta acctggcccg tgcacggatc cagcaccacc ggccgcccgg
     1561 aaatggaccc ggtggtaacc gtatcatcga gcacggagcg attgatcgca taattgcgcg
     1621 gcagcggctc ctgcggacgc aatgtcgccg actgaacgcg cacgttctgc cgcatgctgc
     1681 ccataaacgt attgaactgc tgcacgctga acacggccag gaccgcgacc agaataccga
     1741 caacggcata gtcgcgagcc atcgaacccg aaccgagcaa cgaagaaaga tgacgcatga
     1801 tcccaccaac gattgatcga gccatcatgc atcgacatgg ttaaaaggcg gttacgcccg
     1861 cccctatcca tgatctcttg cggatgcaga tggttgctgg ccaacccctg gggcgcaggc
     1921 tcgtcccgga caggaagcgg cagagcctgg ctctgccgga ataatcggct gtttgccctg
     1981 aggcggctcg ctccggcacc atgttccgcc gccgcgtcag gccatgacga attgcctgac
     2041 cttgcaaagg tatcattcca gtggcccgca caaatgacct gccggtcatt tcccgggccg
     2101 tccctcgcaa ccgggccttc ggccctgctc ggcgccgcat tcgcttctag agcatgtcgt
     2161 gattaagtgg atgccggtta gtcgcagaag acatgctcta acttattgat accgatcagg
     2221 ttatccgcat tttgatgatt ccgtcaaaat gcggcgtgat ccaacccgcc gcctgccaat
     2281 tgcccctggc ggtcagttgt cagggccgct ggtctcagca cgactctagc ttttccgcca
     2341 caagaataaa tgcttacttg cacctgcgat catttgcagc aggcacatga agattacaaa
     2401 acgtttagat tgtgaaaaac gtttaatttg atttcggcgc ctttctggaa tttagtccag
     2461 tcatcgaaac aatcacgatg aggattacat gtccggcatg actgatttca tccgcagacg
     2521 cccgatcatt tcaacccttg tgggggttgc tgttgtgatc ggcggcgttg gcgcggtcag
     2581 cgcccagtat tatggcggcc cgggctcgtg gggccgtggc ggttccggcg gctggggccg
     2641 tggcggcatc gagcgattct gcacggtccc ccacgcacga ttccagccgg tgatgcgcgc
     2701 ctacatcaag gccgacctca acatgacggg cgcccaggct gcggaattcg acaagctggc
     2761 cgatgttctt gctccggcct ttgaggagat caaggctgac ctctgcggca gcttccgggc
     2821 cgattctccc aagatcaccc cgcccgagaa gttggagaag atcgccgcag ccctgcgcaa
     2881 ggcggctacc gctgcagaaa acgcggtcgc tcctgcccgc tcgctctatg gccagctcga
     2941 cgacgcccag aaggctgaga tcgaccggcg catggagcgc cgtcgcatgg cgcgcgaagg
     3001 ccgtggcggt tatggcgaag gccgtggcgg ttacggggaa ggtcgtggca tggggcgcga
     3061 ccgcgacgag caccgtggct ggcacgagca cggcatgggc ccacgccgcg atggtcaggg
     3121 cggatgggag cgcggccccg gccagcctgg tttcggccag agcccggaag gcccgggtgg
     3181 cccgttcttt cgcggcggcc agcaataatt tgccagccac cccttcggcc ctcggttctg
     3241 acccttcggt cattcctgag ggccgttggc cttacgaccg tttgatccag cgcccggcct
     3301 catcctgctg ccagtaggta gcggcatggc cgagcgcctt gaccgccttc cagtcggcgc
     3361 gtgcccgatc gagcgcctcc gggtcgttgc catcgaacat cacgacaacg cgctcgtatg
     3421 gatagtctgc cggcaatggc gcatgatcga ccagaaacag cacctcggcc tggttggggc
     3481 gctcgtcctt ggtggtcagc agcaccggct ggctggccgg atcaccatcc gccgcagttc
     3541 catgggcgat aaagctgtca tccgcaaagg tccacaaaag atcgtcgagt gcctgcaacc
     3601 gctcatccga gccggcgcgc aacaccacct tccagccccg cgcccgtgat ttctcgataa
     3661 gcagcggcag cgactggtcc aaaggccggc tctggagatg gtaaaacagg atttcgctca
     3721 ttgccttgtc cgcgccggcg gatagtgtga cggctcactg tccgagagct gccatgcatg
     3781 attttgatac cgccgccatt ctggcccatc tgcgccgctg ggtcgagatt gaatcgccct
     3841 ccgatgtgcc ttccgccgtc aatgcgatgg tcgatgccct cgccgccgac tacgccagac
     3901 tgccggtgac gcaggagcgc gtcgccggtg ctccggggtt cggcgatcat ctgatcgtcc
     3961 gttcggcatg ggggcaggac cggcccggca ttctcctgct cagccattcc gataccgtgc
     4021 atcctttggg gttcatcctg gaccgcctgc ccttccgcat cgagggggat cgcgcttacg
     4081 ggccgggcat ctacgatatg aagggcgggc tgtgcatcgc ctatcatgcc tttgcggcca
     4141 ttgcggcggc gggcggctcg ccgctcggca tcacccacct gatcgtctcc gacgaggaga
     4201 tcggcagccc ctcgtcccgc ccgttgattg aacggctggg caaggcggcc agccacgttc
     4261 tggtgaccga accggcacgc gacggcggca agatcgttgt cggccgcaag ggcaccggcg
     4321 atttccgcct gaccatccac ggccgcgcct cgcatgccgg caccaagccc gaagaggggc
     4381 gcagcgccgt ccgcgagctt gcgcaccaga tccttcagct tgaagcgctg tccgatccag
     4441 cctccggggt tactgtgacg gtcggcgtgg tccgcggcgg cacgcggccc aatgtcgtgc
     4501 cggaccttgc cgaggcggat gtcgacatcc gcgtgccgac gcttgcggcg gctgatcatc
     4561 tgatcgcagc cattcgcgac cgcaagccgg tgacaccgga tgtgacgatc cgcgtgaccg
     4621 gcgggcttaa ccggccaccc ttcgaacaat cgccggccgg ccgccgcctg ttcgatctgg
     4681 cccatgccga ggcagcgaag atcggcattg atcttgtcgg cgtgttcacc ggcggcggat
     4741 cggacggcaa tttcaccgct ccgaccacgc cgactctcga cggcctcggt gttgacgggg
     4801 ccggcgccca caccgatcag gagcacgcgc tggtctcgtc catggcgccg cgcgccgatc
     4861 ttttgcgccg gatcatgatg acgctgaggt aaggctgccc cgacgattct gccatccggt
     4921 cgatcgtcag ggtcgctggc atcactcgta atattcgctc accagacgat cgagcaggcg
     4981 cacgccccag cccgagcccc acgagcggtt gatgtcggtc gtcggcgagc ccattccggt
     5041 gccggcgatg tcgagatggg cccaggggca atcattgacg aagcgctgga gaaactgcgc
     5101 cgcggtgatc gagccgccat agcggccgcc gctgttcttc atgtcggcga atttggaatc
     5161 gatcatcttg tcgtattcgg cgccgagcgg cagccgccag accttctcgc cggtcatttt
     5221 gccgcaatcg taaaggcgct gggcgagttc gtcattgttg gagaacagcc cggcgtgctc
     5281 ctggccgagc gcgaccagaa tggcgccggt caacgtagcc agattgacca tgaatttcgg
     5341 cttgaacctt tcctggacat accagagtac atcggccagc acgagccggc cctcggcatc
     5401 ggtattgatg atctcgatgg tctggccgga cagcgaggtg acgatgtcgc cgggccgctg
     5461 cgccttgccg tcgggcatgt tctcgaccag gccgatggcg ccgatcacgt tgcatcgcgc
     5521 cttgcgggcc gccagcgcat gcatgagacc gacgacgcag gcagcgcccg ccatgtcgcc
     5581 cttcatatcc tccatgccct gcgccggctt gatcgagatg ccgccggtgt cgaacacgac
     5641 acccttgccg atgaaggcga tcggctgcgc cttggcgtcc ttggcgccgt tccagcgcat
     5701 gatgacgacc tgactgtcct cctccgaacc ctggccgacg ccgagcagcg cccgcatgcc
     5761 gatcttcttc aggtccttct cgccgaggat ttcgatgtcg acacccaact tggccagttc
     5821 gccggcccgg cgggcaaaat cgaccggagt gagcacattc ggcggctcgt tgaccagatc
     5881 gcgcgccaga gtgacaccgt tcagcatgcc gtcacgactg cgcatggccc ggcgagcggc
     5941 ggaataatca gcgaaatgaa ccgcgattgc gccgggctgg ccgctgtcgt catccgcctt
     6001 cttgcgggtc ttgtagcggt cgaaccgata ggaccggagg ccgaggccca gcgcaaaatc
     6061 agccgcctcg tcagcgcccg ccccggccag atcgaacagg atcgacacat cgcggccggc
     6121 cggcagcatt ccggcgacct tgccgccaag cgtgacgaag tcgcga
//
LOCUS       DAOMXG010000146         6089 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_6849_length_6089_cov_8.710474, whole genome shotgun sequence.
ACCESSION   DAOMXG010000146 DAOMXG010000000
VERSION     DAOMXG010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 6089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6089
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6849_length_6089_cov_8.710474"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="PLQ11_09780"
     CDS             <1..586
                     /locus_tag="PLQ11_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRK25229.1"
                     /translation="ELHKLWQAGAPGGYHMEYGYSCMGYEVAGGLGVKMADPAREVFV
                     LVGDGSYLMMNSEIATAVMLGVKITLVILDNRGFGCINRLQMGTGGANFNNLLKDARH
                     ETLPAIDFARHAASLGARAVKVTGIAALEAALKRARRARGVQAIVIDTDPLKSTDAGG
                     HWWDVAVPEVSPRKEVNAARAAYEKALPNQRLGD"
     gene            592..1491
                     /gene="iolE"
                     /locus_tag="PLQ11_09785"
     CDS             592..1491
                     /gene="iolE"
                     /locus_tag="PLQ11_09785"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HRK25230.1"
                     /translation="MTIRIGANPIGWSNDDLLEIGGDTPLETCLAEAKEAGFVGMELG
                     NKFPREAGALKAALAPYGMACVGGWHSVELLKRDAKEEFALAKAHRELLKAMGTSVFI
                     VAETSNAIHGNRAMPLSRRPVLPAGDWKTYCGRMTDLASMLADEGLVLCYHHHMGTIV
                     ESREDIARFMEGCGAPVTLLLDTGHATWGGSDPAELARSYRSRISHVHCKDVRPAVIA
                     EAKAGDWSFLDSILGKGAALGTYTVPGDGAIDYPAVFRELRGYAGWVIVEAEQDPKKA
                     PALPYAKKGVAHLRASLAAAGLV"
     gene            complement(1732..2385)
                     /locus_tag="PLQ11_09790"
     CDS             complement(1732..2385)
                     /locus_tag="PLQ11_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRK25231.1"
                     /translation="MTDTPPPLQIGIIPVTPFQQNCAILRCTATNKAAIVDPGGDADH
                     IVAAVKKMGVTVEKILLTHGHLDHAGGVAGVVAELKVPVEGPHVGDKPLLEGIARQGR
                     MYGVEGLQDAWSDRYLVEGDTVSVGNLIFDVLFVPGHSPGSVVFVSRQAPIAFVGDTI
                     FQGSIGRTDLPGCDHDLLISGIKSKLYALPDETICLSGHGPATTIGDEKRSNPFVRG"
     gene            2578..2769
                     /locus_tag="PLQ11_09795"
     CDS             2578..2769
                     /locus_tag="PLQ11_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012709317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1192 domain-containing protein"
                     /protein_id="HRK25232.1"
                     /translation="MMRDDDDRPRPKLRHEVGQDLSLLSVEEIDDRVALLTEEIERLK
                     ADRAAKLASRSAADAFFKL"
     gene            3128..3652
                     /locus_tag="PLQ11_09800"
     CDS             3128..3652
                     /locus_tag="PLQ11_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1465 family protein"
                     /protein_id="HRK25233.1"
                     /translation="MVSDKTIATISFAQRMVASEGFKALFREGMTLVEETAAYLDGPG
                     RSESRALSRPNALAYASESMRLTTRLMQLTSWLLLQRAVNEGELTRDQAEREHSRIKV
                     SPQDVASSPDMFVLLPVALRSLVERSLRLQDRILHLDADLKQDAGAQAASNAGNPVAA
                     QFNTLRAALNLRQI"
     gene            3879..4730
                     /locus_tag="PLQ11_09805"
     CDS             3879..4730
                     /locus_tag="PLQ11_09805"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRK25234.1"
                     /translation="MAKVKNHKIDGAKNSIIDTSGTLSNPKYLVCHFTGGSTVQSSLD
                     TLKAKGTSYHVLIDRDGTIHQVAPFNVRAAHAGVSNWKGIDGLNEHSIGISLANYGGY
                     LKPLPGKVWRAPGGEELPESRVLVAEHHIGLTQKIAWERFPPAQIAAAIDVIKAVLAA
                     YPSIKDAMGHDEVAMGRREDPGPSFPWADVHALFAARTDFGPVVTVQSPDGSASLRKG
                     RSSSSPELAKLPNGTRLHARSVNYTYVTNAKGEKVPVKHNWVSVARHNTMAHAGFVAR
                     GLLTPQP"
     gene            complement(4791..5021)
                     /gene="rpmE"
                     /locus_tag="PLQ11_09810"
     CDS             complement(4791..5021)
                     /gene="rpmE"
                     /locus_tag="PLQ11_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HRK25235.1"
                     /translation="MKADTHPNYHFIKVVMTDGSEFTTRSTLGKAGDTLNLDIDPKTH
                     PAWTGGTQNLLDRGGRLSKFNNKFAGLSFGKK"
     gene            complement(5230..>6089)
                     /locus_tag="PLQ11_09815"
     CDS             complement(5230..>6089)
                     /locus_tag="PLQ11_09815"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015857427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRK25236.1"
                     /translation="PAAPRAPRYEPLDKPVAAAPQAPRAPRYESLSDPSPNKLRPTAK
                     DSQRAPVAKASKPKVADDDDDDEPATVKAAPKAAAAPAASAGKPTAAQTKAGFKPLEK
                     SQTAAKATTKSSKVASNEDDDAAPAAPKSPVKAAKVEAPKSPAAKADVESTASLGADS
                     QEFRWPARGRVISGYGAKGPNGTNDGINIAVPEGTPVKAAETGTVAYAGEEIKGYGKM
                     VLIRHPNGFVSAYAHNGDLNVKRGDTVKRGQVIAKSGQTGNVTSPQLHFELRKGSEPV
                     DPSRFLEGH"
BASE COUNT         1112 a         1955 c         1906 g         1116 t
ORIGIN      
        1 agaattgcac aagctgtggc aggcgggtgc acccggcggc tatcacatgg aatatggcta
       61 ttcctgcatg ggctacgagg tcgctggcgg actgggcgtg aagatggccg atcccgcccg
      121 cgaggtgttc gtgctggtcg gcgacggctc ctatctgatg atgaattcgg agatcgcgac
      181 cgccgtgatg ctcggcgtca agatcacgct ggtgatcctc gacaatcgcg gtttcggctg
      241 catcaaccgg ttgcagatgg gcaccggtgg cgccaacttc aacaaccttc tgaaggatgc
      301 gcggcacgag accctgccgg ccatcgattt tgcccgccat gccgccagcc tcggcgcaag
      361 ggcggtgaag gtgaccggca tcgccgcgct cgaagccgcg ctgaaacgcg cccgccgggc
      421 caggggcgtg caggccatcg tcatcgatac cgatccgctc aaatcaaccg atgccggcgg
      481 gcattggtgg gatgtcgcgg tgccggaagt ctcgccgcgc aaagaagtca acgccgcccg
      541 cgccgcttat gagaaggcgc tgcccaacca gcggctcgga gattgaacgt tatgaccatt
      601 cgcatcggcg ccaatcccat cggctggtcc aacgacgacc ttctcgaaat cggcggcgac
      661 acgccgctgg aaacctgcct cgccgaggcg aaggaggccg ggttcgtcgg catggagctt
      721 ggcaacaagt tcccgcgcga ggccggcgca ctgaaggcgg cactcgctcc ctacggcatg
      781 gcctgcgtcg gtggctggca ttcggtcgaa ctgctcaagc gcgatgcaaa ggaggaattt
      841 gccctcgcca aggcccaccg cgagctactc aaggccatgg gcaccagcgt gttcatcgtc
      901 gccgagacct ccaacgccat ccacggcaac cgcgcgatgc cactcagccg ccgccccgtg
      961 ctgccggccg gcgactggaa aacctattgc gggcggatga ccgacctggc ctccatgctg
     1021 gccgacgaag ggctggtgct ctgctaccat caccacatgg gcaccatcgt cgaaagccgc
     1081 gaggatatcg cccgcttcat ggagggctgc ggcgcaccgg tcaccctgct gctcgatacc
     1141 ggccatgcca cctggggcgg ctcggacccg gcagagctcg cccgcagcta ccgcagccgc
     1201 atcagccatg tgcattgcaa ggatgtacgc cccgcggtga tcgccgaagc aaaagccggc
     1261 gactggagct ttcttgattc catcctcggc aaaggcgcgg cgctcggcac ctataccgtg
     1321 ccgggggacg gggccatcga ctatccggcg gtgttccgtg aactcagggg ctatgccggc
     1381 tgggtgatcg tcgaggccga gcaggacccg aagaaggcgc ccgccctgcc ttacgcgaaa
     1441 aaaggtgtcg cccatctccg ggccagcctc gccgccgccg gcctggtgta attcccagga
     1501 gcaaagcgac gccgaacctt tgcgaggggc accacacccg ctctttcctt ccccttgatg
     1561 cggagggtcg gcgcgtcagc gccggtgagg ggtgagaggg tgaaccaccc ccacccggtc
     1621 ctccacacgc agtcaagtct acgcagactg cgtaaacttg tctgcgatgc tcggaccacc
     1681 ctccccgcaa gggggaagga ttttttgcgg caagggccac aataaaccct cctacccccg
     1741 cacgaaggga ttggaccgct tctcgtcgcc gatcgtggtg gccgggccat ggccggagag
     1801 gcagatcgtt tcatccggca gggcatagag cttggacttg atgccagaga tcaacaggtc
     1861 gtggtcgcag cccggcaggt cggtgcggcc gatcgagccc tggaaaatcg tatcgccgac
     1921 gaaggcgatc ggcgcctgcc ggctgacgaa caccaccgag cccggcgaat ggccgggcac
     1981 gaacagcaca tcgaaaatga gattgccgac gctgaccgta tcgccctcga ccaggtagcg
     2041 gtccgaccag gcatcctgca aaccctcgac gccatacatg cgcccctgcc gggcaatgcc
     2101 ttccagcagc ggcttgtcgc cgacatgcgg cccttccacc ggtaccttca actccgcgac
     2161 gacaccggcg accccgccgg catgatcgag atggccatgc gtcagcagga tcttctcgac
     2221 cgtcacgccc atcttcttca cggcggcgac gatatgatcg gcatcgccgc cgggatcgac
     2281 gatcgccgcc ttgttggttg ccgtgcagcg caggatggcg cagttctgct ggaacggcgt
     2341 gacaggaatg atgccgatct ggagcggcgg cggtgtgtcg gtcatggcat ccccggtggg
     2401 ctggcgttgc cccgagcgat agtcgcttga accgcggccg gcaaggtcga aaggacgcca
     2461 ttccgccaag ccacgcgacc gcccgtcccg ttgcacgcgc cggcgaccca ttataggact
     2521 cgtccgtcag gctttggcac gggcgtcccg cgacgctgtg ccagcaggga gacccacatg
     2581 atgcgcgatg atgatgatcg gccccgccca aagcttcgcc atgaggtcgg tcaggacctg
     2641 tcgctgctgt cggtcgagga gatcgacgac cgcgtcgcgc tgctgaccga ggagatcgag
     2701 cgactcaagg ccgaccgtgc cgccaagctg gcctcacggt cggcggcgga tgcgtttttc
     2761 aagctttgac cggattttac cgtccggatc attttgaaac cgctaaaatt tggcgaattt
     2821 accgattatt tacggctctc gtttagggtg atgactgtcc agttttgctg gaccgagtgg
     2881 ctcctgtcac tctgtttgac gcctccctgt tatcactttc aaagggctgc ctccgcagcc
     2941 ctcttttttt tgcccgcgct gcgcggcccg aaattaacca taatgaactg ttgctcatgg
     3001 cggttatggt taaaggcgct atagattgca tccgtctctg ccgacaccca aagctgtcgc
     3061 aaactgaaac gagaacagtc cgttcggacg gagacaagaa atgcaggatg tgcgttgcga
     3121 tttatcgatg gtttcggaca agacaattgc cacgatttcc ttcgcccagc ggatggtggc
     3181 atccgaaggg ttcaaggcgt tgttccgtga aggcatgacg ctggttgagg aaacggccgc
     3241 ttatctggac ggtcccggcc ggtcggaatc gcgcgccctg tcgcgcccca atgccctggc
     3301 ctatgcttcg gaaagcatgc gcctgaccac ccgcctgatg cagctgacat cgtggctgct
     3361 gctccagcgc gccgtcaacg agggcgagct gacccgtgat caggccgagc gcgagcacag
     3421 ccgcatcaag gtctcgccgc aggacgtcgc ctcctcgccc gacatgttcg tgctgctgcc
     3481 ggtggcgctg cggtcgctgg ttgaacgctc gctgcgcctg caggatcgca tcctccatct
     3541 cgatgccgat ctcaagcagg acgccggcgc ccaggctgcc tcaaacgccg gaaatccggt
     3601 cgcggcccag ttcaacaccc tcagggccgc cctgaacctg cgccagatct gagcccggca
     3661 ctccgctaga tcaggccgca ttttgatgga atcatcaaat gcggataacg tgatcggtat
     3721 caataagtta gagcatgtct tctgcgattg accggtatcc acttaatcgc gacatgctct
     3781 agccaccagc gcctcacccg tttggatgtt tgcgaacctg caactctctc tagccttggc
     3841 ctggtcatcg acggagccaa ggcgcgaggg aggcaaacat ggcgaaagtc aagaaccaca
     3901 agattgacgg ggccaagaac agtatcatcg atacgtcggg cactctgtcc aaccccaaat
     3961 accttgtctg ccacttcaca ggcggcagca cggtgcagag ttcgcttgat accctcaagg
     4021 ccaagggcac cagctatcat gtgctgattg acagggacgg cacgatccat caggtcgccc
     4081 cgttcaatgt cagggcagcc catgccggcg tcagcaactg gaagggcatc gacgggctga
     4141 atgaacattc gatcggcatt tcgctggcca attacggcgg ctacctgaag cccctgccgg
     4201 gcaaggtctg gcgcgcaccc ggcggcgagg aactgccgga aagccgcgtt ctggtggccg
     4261 agcaccacat cggcctgacc cagaagattg cctgggaacg ctttccgccc gcacagatcg
     4321 ccgcagccat cgatgtgatc aaggcggtgc tcgccgccta tccctcgatc aaggatgcca
     4381 tggggcatga cgaggtggcc atgggccgcc gcgaggaccc aggcccatcc ttcccctggg
     4441 cagatgtgca tgcgctgttc gccgcacgca ccgatttcgg cccggtggtg acggtgcaat
     4501 caccggacgg gtctgccagc ctgcgcaagg gccgcagcag cagttcaccc gagcttgcca
     4561 agctgccaaa cggcaccagg ctgcatgccc gttcggtcaa ctatacctat gtcaccaacg
     4621 ccaaaggcga gaaggtcccg gtcaagcaca actgggtctc ggtcgcgcgc cacaacacga
     4681 tggcacatgc cggatttgta gcccgcggac tgctgacgcc gcagccatga cagacagcgc
     4741 acaaaaaaag gccccgtttc cggggccttt tccattgcgg gctgggccgc ttatttcttg
     4801 ccgaatgaca ggccggcgaa cttgttgttg aacttcgaca ggcgaccgcc gcgatcaagc
     4861 aggttctgtg tgccgccggt ccaggccgga tgcgtcttgg gatcgatgtc gagattgagc
     4921 gtatcgcccg ccttgccgag cgtcgagcgg gtggtgaact cgctcccgtc ggtcatcacg
     4981 accttgatga aatggtagtt gggatgggta tcggccttca tggcgggtct ccggaggtcg
     5041 ggccgcccgg cggccatgcg acatcgatgc aattgaagaa gcgcccctat agacgagttc
     5101 ggcgaacggg gcaagcagcc cgcaggcagt cggtgggctc aaaaaagggc gatggcggcg
     5161 gatcgctccg ccgccatgcc tttttcatcc ggcagggtct cgcgtttgcc gggtgaaact
     5221 cgatggaggt cagtgccctt cgaggaaacg cgacggatcg accggctcgg agcccttgcg
     5281 caactcgaag tgaagctgcg gcgaggtcac attgcccgtc tgtccggact tggcgatcac
     5341 ctgcccgcgc ttgaccgtat cgccgcgctt gacgttgaga tcgccgttat gggcataggc
     5401 cgaaacgaaa ccgttgggat ggcggatcag caccatcttg ccatagcctt tgatttcctc
     5461 gccggcatag gccacggtcc ccgtctcggc ggccttgacc ggcgtgcctt ccggaactgc
     5521 gatattgatc ccgtcattgg tgccgttcgg ccccttggcg ccatagccgg agatcacccg
     5581 gccgcgcgcc ggccagcgga attcctggct gtcggcgccg agcgaagccg tgctttcgac
     5641 atcggccttg gcagccggcg acttgggcgc ctcgaccttg gcggccttga ccggagactt
     5701 cggagcggcc ggagccgcat cgtcatcctc gttgctggca accttcgagg atttcgtcgt
     5761 cgccttggcg gcagtctgtg atttttcgag cggcttgaag ccggccttgg tctgcgcggc
     5821 agtgggcttg cctgccgacg ccgccggcgc tgccgcagcc ttgggagctg ccttcacggt
     5881 cgccggttca tcgtcatcat cgtcatcggc gaccttcggc ttgctggcct ttgccaccgg
     5941 agcccgctgg ctgtccttgg ctgtcggacg cagcttgttg ggcgagggat cggacaggga
     6001 ttcatagcgt ggcgcacgcg gcgcctgggg cgctgcggca accggcttgt cgagcggctc
     6061 atagcgcggt gcacgcggtg ccgccggag
//
LOCUS       DAOMXG010000147         6021 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_6959_length_6021_cov_10.431277, whole genome shotgun sequence.
ACCESSION   DAOMXG010000147 DAOMXG010000000
VERSION     DAOMXG010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 6021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6021
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6959_length_6021_cov_10.431277"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(398..1069)
                     /locus_tag="PLQ11_09820"
     CDS             complement(398..1069)
                     /locus_tag="PLQ11_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HRK25237.1"
                     /translation="MSTARADILGNIRRSLGVDGNERIRLAQVEDRLERSPRGVIPAR
                     GQLEPEARVALFKDRAEAVQATVDHLARLEDIPVAIAAHLRNSNLPAELRIGADALLT
                     GLDWDKTAIRVSRGPSDGRDLVAVSHAIAGVAETGTLVLTSGQDNPTTLNFLPDTHIV
                     VVKAVDIAADYEAVWARLRHSYGKGVMPRTVNYVTGPSRSADIEQKLQLGAHGPRNLH
                     ILIVG"
     gene            complement(1066..2493)
                     /locus_tag="PLQ11_09825"
     CDS             complement(1066..2493)
                     /locus_tag="PLQ11_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HRK25238.1"
                     /translation="MSVQSTSHDFKNNTHRALGDVKLQQAMGFVRVGFIEKRAAAAAR
                     LPEFEALRDSAREIKTHTLKHLDLYLERYEEKVTESGGKVHYARDAEEARAVILDICK
                     SVNARIVTKGKSMVAEEIGLNAHLEAHGLVPVETDLGEYIIQLRGEMPSHIIAPAVHV
                     TQGQVEAEFRRVHTHLDPGRDLSEPSSLLAEARGVLRDRFLAADVGITGANFLVAETG
                     TSIIVTNEGNGDLTQTLPKVHIVIASIEKVVPTLEDASQILRVLARSATGQDMSVYTT
                     FSTGPRRADDPDGPGQYHVVILDNGRSAMLGGQFEDMLRCIRCGACMNHCPVYHAIGG
                     HAYGWVYPGPMGAVLTPSLIGVDKAGHLPNASTFCGRCENVCPMRIPLPRMMRNWREK
                     EFERHLSPAVARFGLGAWAFLARRPWLYSLAMRPAIWAMARMGARKGRFSFLPLAGGW
                     TKYRDLAAPEGETFQARFRRQRSGR"
     gene            complement(2566..3768)
                     /gene="pcaF"
                     /locus_tag="PLQ11_09830"
     CDS             complement(2566..3768)
                     /gene="pcaF"
                     /locus_tag="PLQ11_09830"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HRK25239.1"
                     /translation="MIDAFICDYVRTAIGRFGGSLAKVRTDDLAAAPIRELLRRHPAL
                     AGAVEDVYYGSANQAGEDNRDVARMAALLAGLPDTTPGVTLNRLCASGMDAVGAAARA
                     IRADEIGLAIAGGVESMTRAPLVMGKAEVAFQRSAEVFDTTIGWRFINPLMKKQYGVD
                     SMPETAQNVADEFDVSRADQDAFALRSQQRAGRAMVDGFFDGEIVPIEVGDARGNVIA
                     FARDEHARPDTTAEQLAKLKPIVRPDGSVTAGNASGVNDGAAALVIASARAADRLGLR
                     PMARILGMQSVGVAPRIMGIGPAPATQKLCDRLGIKVSALDVVELNEAFAAQGLASTR
                     MLGLPDDAAHVNPNGGAIALGHPLGMSGARICGTAARELIRRGGRYAVATMCVGVGQG
                     VALAIERV"
     gene            complement(3871..4623)
                     /locus_tag="PLQ11_09835"
     CDS             complement(3871..4623)
                     /locus_tag="PLQ11_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRK25240.1"
                     /translation="MNRIDLASRVAIITGGARGIGYATAERMLASGASVALWDMDKEA
                     LKKAAGKLFARGTVTVHTVELTSEAAVTEATEAVIGRHGKIDILINNAGITGGNGKLW
                     ELAPDIWRKVVEVNLIAPYLTCRAVVPKMLEKGYGRIVNVASIAGKEGNPNASHYSAS
                     KAGLIGLTKSLGKELATANILVNCITPAAARTDIFDQMKPEFIQFMLSKIPMNRFVEV
                     DEIAAMIAFLSSQECSFTTGGVFDISGGRAVY"
     gene            complement(4634..5476)
                     /locus_tag="PLQ11_09840"
     CDS             complement(4634..5476)
                     /locus_tag="PLQ11_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018430405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRK25241.1"
                     /translation="MKLVRFGGVGSERPGILDSSGAIRDLSGVVDDIAGATLTPEGLA
                     ALRKVDVERLPLAPAGVRIGPCVGAIGKFICIGLNYADHAAETGSPIPAEPIVFMKAT
                     SAIIGPNDTVEIPRASKKTDWEVELGVIIGKPAKYVAEAEAMSHVAGYCVINDISERE
                     FQIERGGTWDKGKGCDTFGPIGPWMVTADEVKDPQNLAMWLEVDGKRYQNGSTRTMIF
                     GVVRLVSYVSQFMSLQTGDVISTGTPPGVGLGQKPPVFLRAGQTMRLGIEGLGEQRQT
                     TVQA"
     gene            complement(5565..>6021)
                     /locus_tag="PLQ11_09845"
     CDS             complement(5565..>6021)
                     /locus_tag="PLQ11_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HRK25242.1"
                     /translation="GVTAGWAATGVLVDPFHPSIRLQSLTFVAPVGRAFYGAMMGGPN
                     WLDFGVATVPGVIAGAFLASWAGNELRWEAFDDPREMRRHVLGAAMMGIGGVLAGGCT
                     IGQGLTAASLTAATAPLAIAGFMLGARIGILFLVESSLRDMLAHLFARR"
BASE COUNT         1107 a         1983 c         1907 g         1024 t
ORIGIN      
        1 gttgccggtg gagcggacgc cgacggagcg atggccggcg ccggagcagg gcttggtgca
       61 ggcacagcga caggcgccgg ggctttctgt tcggtcgtcg cgggcgccgt ctgcgccagg
      121 gcagacccgg cgagcgccag cacaacagca gcagcggaga cggtgttacg catcatgctc
      181 tcttcgttcg acctgacagg tccagacttg ccccgagggc acatcatgac ctgttatggt
      241 taccgagctg ttggcgccgc cctcgccaat cgcctctcaa tgacacaacc ggccaagatt
      301 tcgcgagaaa tatggcaagc ccgcgccagc gccccgacct gccgggtcgt tacagggctg
      361 atttgccccg actgttgcaa atgagcgacg gtgaggctca gccgacaatc agaatatgca
      421 gattgcgggg gccatgcgcg cccagttgca atttctgctc gatgtcggcg gagcgcgatg
      481 gcccggtgac ataattgacc gttcgcggca tcacgccctt gccgtagctg tggcgcagcc
      541 tggcccagac agcctcataa tcggcggcga tatcaacagc cttgaccacc acgatatggg
      601 tatcgggaag gaaattcagc gtcgtcgggt tgtcctggcc cgaggtcaga accagcgtcc
      661 cggtctcggc aacgccggcg atggcatggc tgacagcaac cagatcgcgg ccatcgctcg
      721 gcccacgcga aacgcggatc gccgtcttgt cccagtcaag cccggtcagc aatgcatccg
      781 caccgatgcg cagttccgcc ggcaggttgc tgttgcgcag atgggcggca atggccaccg
      841 ggatatcctc gagacgcgcc agatggtcca ccgtcgcctg aacggcttcg gcacgatcct
      901 tgaacagcgc gacgcgcgcc tccggctcca gttgcccgcg cgccggaatg accccgcgcg
      961 ggctgcgctc cagccggtcc tcgacctgcg caagacggat gcgctcgttg ccgtcaaccc
     1021 cgagcgagcg gcggatgttg cccagaatat cggcgcgcgc tgtgctcatc gaccgctcct
     1081 ctgccggcgg aacctggcct ggaacgtctc gccttccggc gcggcgagat cgcggtattt
     1141 cgtccagccg ccggccagcg gcaggaagga gaagcgtccc ttgcgcgcgc ccatcctggc
     1201 catggcccag atggccgggc gcatggcaag gctgtaaagc cagggccggc gggcgagaaa
     1261 tgcccaggcg cccagcccga agcgggcgac ggccggtgac agatggcgct cgaattcctt
     1321 ctcgcgccag ttccgcatca tcctgggcag cgggatccgc atcggacaga cattttcgca
     1381 gcggccgcag aaggtcgagg cattgggcag atgccctgcc ttgtcgacac cgatcagcga
     1441 cggcgtcagc accgcgccca tcggccccgg atagacccag ccataggcat ggccgccaat
     1501 cgcatggtag accgggcaat ggttcatgca ggcgccgcag cggatgcagc gcagcatatc
     1561 ctcgaactgc ccgccgagca tggcagagcg gccattgtcg agaatgacga catgatattg
     1621 gcccgggccg tccggatcat cggcgcggcg gggaccggtc gaaaacgtcg tatagaccga
     1681 catgtcctgg cccgtggccg agcgcgccag aacgcgcagg atctggctgg catcctccag
     1741 cgtcggcacc accttctcga tcgaggcaat gacgatgtgg actttcggca gggtctgggt
     1801 gagatcgcca ttgccctcgt tggtgacgat gatcgaggtt ccggtctcgg ctaccaggaa
     1861 attggcgccg gtgatgccga catcggccgc cagaaaacga tcgcgcagca cgccgcgcgc
     1921 ctcggccagc agactggacg gctcgctcag gtcgcgaccc ggatcgagat gcgtgtggac
     1981 gcggcggaat tcggcctcga cctgcccctg cgtgacatgc accgccggtg cgatgatatg
     2041 cgagggcatc tcgccccgca gctggatgat gtattcgcca aggtcggtct cgaccggaac
     2101 aagaccatgg gcttccagat gggcattgag gccgatctcc tcggccacca ttgacttgcc
     2161 cttcgtgacg atacgcgcat tcaccgattt gcagatgtcg aggatgaccg cacgggcctc
     2221 ctcggcatcg cgggcatagt gcaccttgcc gcccgattcg gtgaccttct cctcgtagcg
     2281 ctcgagatag agatcgagat gcttgagcgt atgggtcttg atctcccgcg cgctgtcacg
     2341 cagcgcctcg aactccggca ggcgtgccgc agcggccgcc cgtttctcga tgaagccgac
     2401 gcggacaaag cccatcgcct gctgcagctt cacatcgccg agtgcccgat gggtattgtt
     2461 cttgaagtcg tgtgaggtgg actgaacact catgcccggc gcctagctcc cgatcgttgc
     2521 cgacagagta gcgggaagca tgcagcgagg aaagggcgga tcggctcaca cccgttcaat
     2581 cgccagcgcc acgccctgcc ccacgccgac gcacatcgtc gccaccgcat agcgaccgcc
     2641 gcggcggata agttcacgcg ccgccgtgcc acagatgcgc gcgccggaca tgcccagcgg
     2701 atggccgagc gcgatcgcgc cgccattcgg attgacatgg gcggcatcgt ccggcaatcc
     2761 aagcatgcgg gtgctggcca gcccctgagc ggcaaaagcc tcgttgagtt cgaccacatc
     2821 cagcgccgac accttgatgc cgagcctgtc gcaaagtttc tgcgtcgccg gcgccggtcc
     2881 gattcccatg atgcgcggcg caacgccaac gctctgcatg ccgagaatgc gcgccatcgg
     2941 cctgaggccc agcctgtcgg cagccctggc ggaggcaatc accagcgcgg cggcaccgtc
     3001 attgacgccc gaggcattgc ccgccgtaac cgatccatcg gggcggacga tgggcttgag
     3061 cttggcaagc tgctcggcgg tggtgtcggg ccgggcatgc tcgtcgcggg caaaggctat
     3121 gacattgccc ctggcatccc cgacctcgat gggcacgatt tcgccgtcga agaacccgtc
     3181 caccatcgcc cgcccggcac gctgctgcga ccgcagcgcg aaagcatcct gatcagcgcg
     3241 cgaaacatcg aactcgtcgg cgacgttctg ggcggtttcc ggcatcgaat cgacaccata
     3301 ttgtttcttc atcagcgggt tgatgaaccg ccagccgatc gtggtgtcga atacctcggc
     3361 cgaacgctgg aaggccactt ccgccttgcc catgacgagc ggcgcacggg tcatcgattc
     3421 aacgccgccg gcaatggcta gcccgatctc atcggcgcgg atggcgcgcg ctgcggcacc
     3481 gaccgcatcc attccggagg cgcagagacg gttgagcgtc acaccgggcg tcgtatcggg
     3541 caggccggcc agcagggccg ccatgcgggc cacgtcgcga ttgtcctcac cggcctgatt
     3601 ggcagagccg taatagacat cctcgaccgc gccggcgagc gcagggtgac ggcgcaacag
     3661 ttcgcggatc ggggcagcgg caagatcgtc ggtgcggact ttagccagcg acccaccaaa
     3721 acggccgatg gccgtgcgga catagtcaca gatgaatgca tcgatcatgg aaagcctccc
     3781 gtttcccgcc gttgtcaccc ggccagaaag gaaaatcaag acattcgttg accgatcgtt
     3841 cagcgaattg cggaggggac cgtgctagcg tcagtagacg gcgcggccac cggaaatgtc
     3901 gaacaccccg ccggtggtga acgagcattc ctgagaggac agaaaggcga tcatcgcggc
     3961 gatctcatcc acctcgacaa agcggttcat cgggatcttc gacagcatga actggatgaa
     4021 ttccggtttc atctggtcga agatatcggt tctggcagcc gccggcgtga tgcagttcac
     4081 cagaatgttg gcggtcgcca gctccttgcc gagcgacttg gtgagcccga tcaggccggc
     4141 tttcgaagca gaatagtgtg aggcattggg gttgccttcc ttgccggcga tggaggcgac
     4201 gttgacgatg cggccatagc ccttctcaag cattttcggc acaaccgcgc ggcaggtcag
     4261 gtagggggcg atcagattga cctcgaccac cttgcgccag atgtcggggg cgagttccca
     4321 cagcttgcca ttgccgccgg taatgccggc gttgttgatc aggatgtcga tcttgccgtg
     4381 ccgtccgatg accgcctcgg tcgcctcagt tacagcggcc tcggaggtca gctcaaccgt
     4441 atgcacggtc accgtgccgc gggcgaacag cttgcccgcc gccttcttga gcgcttcctt
     4501 gtccatatcc caaagcgcca ctgaagcacc ggacgccagc atgcgttcgg cggtggcata
     4561 gccaatgccc cgggcgccgc cggttatgat tgcgacgcgg ctggcaagat cgatccggtt
     4621 catgcgagcc cccttatgcc tgcacagtgg tctgccgctg ctcacccagc ccctcgatgc
     4681 cgaggcgcat ggtctggccg gccctaagga agaccggcgg cttctggccc aggccgacgc
     4741 ccggcggcgt gccggtcgag atcacatcgc cggtctgcag gctcatgaac tggctgacat
     4801 aggagacgag cctgacgacg ccgaagatca tcgtgcgggt ggagccgttc tggtagcgtt
     4861 tgccgtcgac ctcgagccac atggccagat tctgcgggtc cttgacttcg tcggcggtca
     4921 ccatccaggg gccgatcggg ccgaaggtat cgcagccctt gcctttgtcc cacgtcccgc
     4981 cgcgctcgat ctggaattcg cgctcggaaa tgtcgttgat gacgcaatag ccagcgacat
     5041 ggctcatcgc ctcggcctcc gcgacatatt tggccggctt gccgatgata acgccgagtt
     5101 cgacctccca gtcggtcttt ttcgaggcgc ggggaatttc caccgtgtca ttcgggccga
     5161 tgatggcgct ggtcgccttc atgaagacga tcggctcggc aggaatgggc gagccggtct
     5221 ccgccgcatg atcggcatag ttgaggccga tgcagatgaa cttgccgatg gcgccgacgc
     5281 acggcccgat ccggacaccg gccggagcca gcggcaacct ctcgacatcg accttcctca
     5341 gcgcggccag cccttcgggc gtcaatgtcg ccccggcgat gtcgtccacc acaccgctga
     5401 ggtcccggat agccccggac gaatcgagga tgccggggcg ttccgagccg accccaccaa
     5461 aacgcacaag cttcatgata cctccagcaa gccgatttcg gccaccccgt gctggcgacc
     5521 gcagacggtt gtatgacaac tctggattcg tctgacaatc gttttcagcg tctggcaaac
     5581 agatgggcca gcatgtcgcg cagcgagctt tcgacgagga aaagaatgcc gatccgcgcg
     5641 ccgagcatga agcccgctat ggcaagcgga gccgtggccg cggtgagcga agctgctgtc
     5701 agcccctggc cgatggtgca gccgccagcc aggaccccgc cgatgcccat cattgctgcc
     5761 ccaaggacat gacgccgcat ttcacgcgga tcatcgaagg cttcccagcg cagctcattg
     5821 ccggcccaac tggccagaaa ggcaccggca atcacgccgg gcacggtggc aacgccgaaa
     5881 tcaagccagt tcggtcctcc catcatcgcg ccgtagaacg cccggccgac cggtgcaacg
     5941 aaggtcaggc tctgcaagcg gatggacgga tggaacggat cgacaagcac gcccgtcgcc
     6001 gcccagccgg cggtcacgcc c
//
LOCUS       DAOMXG010000148         5895 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_7149_length_5895_cov_8.741096, whole genome shotgun sequence.
ACCESSION   DAOMXG010000148 DAOMXG010000000
VERSION     DAOMXG010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 5895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5895
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7149_length_5895_cov_8.741096"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..1535)
                     /locus_tag="PLQ11_09850"
     CDS             complement(105..1535)
                     /locus_tag="PLQ11_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HRK25243.1"
                     /translation="MNAIAFPKPRADILARRDAIIAGLARLVEPAGLITTANECTAFE
                     TDAFTAYRRRPLAVVLPASTAEVSAVLAYLNSEGIPVVPRGAGTSLCGGATPQEDAVV
                     IGVSRLNRILGVDYPNRTVRVEAGVTNLAISNAVDADGYFYAPDPSSQLACTIAGNIG
                     MNSGGAHCLKYGVTTNNLMGVKLVLLDGTVIDIGGEHLDAGGLDLLGLVCGSEGQLGI
                     ITEATVRILRKAEGARPVLFGFPSAEAAGECVAAVIGAGIIPVAMEFMDRQAIAIVED
                     FAKAGYPLDAEALLIIEVEGSEAEMDATLARIVEIAQRHKVQTIRESKSAMETAAIWK
                     GRKSAFGATGRVADYICMDGTVPTGQLAHVLKQTDVIVQKYGLRVANVFHAGDGNMHP
                     LILYNVNDPEEARKAEAAGMDILRLCVDVGGCLTGEHGVGIEKRDLMLHQFSKADLDQ
                     QMSVRAVFDPAWLLNPAKVFPLEGRP"
     gene            complement(1595..2911)
                     /locus_tag="PLQ11_09855"
     CDS             complement(1595..2911)
                     /locus_tag="PLQ11_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3422 domain-containing protein"
                     /protein_id="HRK25244.1"
                     /translation="MAEVRPAPAAAAQTFTPHPQRGPVLGEVHARPFYPVPTPSRILR
                     FAFTTDWEQSKKDRGALTAFCQSRGVQGPAEGLKHLRVTLSDAVLRWEQHSEFTTYTF
                     EIGGTDRTPFERPAGQLAQVMRDIPQPGPLLSAIDLHLVDTMPDGGLEGVFDAMSLAA
                     STIDGGIATAASDFKVTSDGFVRFLIINQNLTPDTAGRLTQRLLEIETYRVFALLGLP
                     TAQAVTPALRKAEEELTDIARAMTEATGFDGNHAQLERLMQLASSLELESSRTDYRFS
                     AVRAYEGIIDQRVESLRETPLPLGYPTFATFLARRVKPALRTCHMVAERQSRLAEKIA
                     RNANLLRTRVQVDLEQQNRDSMQSMNERARLQLRMQQTVEGLSVAAVSYYVLGLLGYL
                     FKGAKDAELTKLDSSVLTALALPFVIFGVAMAVRRIRKQHSEHDHG"
     gene            2983..5535
                     /locus_tag="PLQ11_09860"
     CDS             2983..5535
                     /locus_tag="PLQ11_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response DEXH box
                     helicase"
                     /protein_id="HRK25245.1"
                     /translation="MTRDNSPGSTGCARLPRCLADWFSARGWRLRQHQADLIDLAAQG
                     RSALLIAPTGAGKTLAGFLPSLISLSEPGKRRRGGLHTLYVSPLKALAVDITRNLEAP
                     VGETGLPIRIETRTGDTPASRRQRQRRDPPDMLLTTPEQIALMLSGSDAEHLFAALDR
                     IIVDEAHALAPIKRGDQLALCLSRLRRLKPDVTVTGLSATVRDPVALARWLAPEATIV
                     TAGQDAAAAAVLDILDCGAPLPWAGHIARHAVEAIYSAIGGHRLSLIFVNTRMQAEFL
                     FQRLWEINEDGRRIALHHGSLDAGQRRKVEAAMAAGRLDAVVATSTLDLGIDWGDVDL
                     VINVGAPKGASRLIQRIGRANHRLDTPSKALLVPANRFELLECRAAIAAARAGTQDAD
                     VIRTGGLDVLAQHVLASAAAGPFDAEALFAEVRSAAPYADLTREDYDAIVDFVATGGY
                     ALKTYERFAKIRRTRDGLWRIANPAVAQTFRMNVGTIVEEAMLKVRLGRARSTGSGPT
                     GPIGRAGRLLGEVEEYFAEQLQPGDTFAFGGEILAFQALVDNEVIVARTSARDPKVPS
                     FMGGKFPLSTHLSERVRAMLAEPATWGDFPAEVRNWLTLQQAVSRLPGRADLLVETFP
                     RGGRFYMVAHAFEGRLAHQTLGMLLTRRLERARLQPMGFVCNDYALCIWGARDIGLAA
                     AHDPDLFGHLFDEDMMGDDLEEWLAESSIMKRTFRNAAIIAGMIERRFPGAEKGRRQL
                     TMSSDLIYDVLRRHQPDHILIKAARADAASGLIDARRLGELLVRIAGRIVHKRLERVS
                     PLAVPVMLEIGREQVYGEAADRLLDEAALDLEREALGGLATTAIGGARADAG"
     gene            5525..>5895
                     /locus_tag="PLQ11_09865"
     CDS             5525..>5895
                     /locus_tag="PLQ11_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRK25246.1"
                     /translation="MPAEIMLGGASVRLDVSGCAMLIGEGALLVADLHLEKGSSYARG
                     RQFLPPYDTLATLARLSEVVARLAPRQIVVLGDAFHDRHAGERLPREAVAAIGALATG
                     RRLVWIAGNHDPAPPVGLPGER"
BASE COUNT         1004 a         1954 c         1944 g          993 t
ORIGIN      
        1 ggccgcgcgc gtgccgccgc cgagaatgcg caaagccgtg ccggcctgac gcgccgccat
       61 caccgcgtcg acgacctcag cctccgttgt tggattgtaa gacgtcacgg acgcccctcc
      121 agcggaaaga ccttggccgg attgagcagc caggccggat cgaacaccgc gcgcaccgac
      181 atctgctggt cgaggtcggc cttcgagaac tggtggagca tcagatcgcg cttctcgatg
      241 ccgacgccgt gctcgccggt cagacagccg ccgacatcga cgcaaagcct gaggatatcc
      301 atgcccgccg cttccgcctt gcgggcttct tcgggatcat tgacgttgta gaggatcagc
      361 ggatgcatgt tgccatcgcc ggcgtggaac acattggcga cgcgcagccc gtatttctgc
      421 acgatgacat cggtctgctt aagcacatgg gcgagctggc cggtgggcac ggtgccatcc
      481 atgcagatgt aatcagccac gcggccggtc gccccgaagg cggatttccg ccctttccag
      541 atggccgccg tctccatagc cgacttgctc tcgcgaatgg tctgcacctt gtgccgctgc
      601 gcgatctcga cgatgcgggc gagcgtcgcg tccatttccg cttcggagcc ttcaacctcg
      661 atgatcaaca gcgcttcggc atcgagcgga tagccggcct tggcaaaatc ctcgacaatg
      721 gcaatggcct gcctgtccat gaattccatc gccaccggaa tgatgccggc gccgatgacg
      781 gcggcgacac attcaccggc agcttccgcg ctgggaaagc cgaacagcac cgggcgggcg
      841 ccttcggcct tgcgcaggat gcgcaccgtc gcctcggtga tgatgccgag ctggccctcc
      901 gagccacaga caagaccgag caggtcaagc ccgccggcgt cgagatgctc accgccgatg
      961 tcaatcaccg taccatcgag cagcaccagc ttgacgccca tcaggttgtt ggtcgtcacc
     1021 ccgtatttca ggcaatgggc gccaccggaa ttcatgccga tgttgccggc gatggtgcag
     1081 gcgagttgcg aggacggatc gggcgcatag aaatagccgt ccgcatccac agcattggaa
     1141 atcgcaaggt tggtcacgcc cgcctcgacc cgcacggtgc ggttggggta gtcaacgcca
     1201 aggatgcggt tgaggcgaga aacgccgatg acgaccgcat cctcctgcgg ggtcgctccc
     1261 ccgcacagcg aggtgccggc gccgcgcgga acgaccggga tcccctcgct attcagatag
     1321 gccaggacag cgctgacctc ggcggtcgag gccggcaaca ccaccgccag cggcctgcgc
     1381 cggtaggcgg tgaacgcatc cgtctcgaag gcggtgcatt cgttggccgt ggtgatcagc
     1441 ccggcaggct cgaccagccg ggccagcccg gcgatgatcg catcccgtct cgcaagaatg
     1501 tcggcacgcg gctttggaaa agcgatggcg ttcatgggcg tctccctgcc atggactgta
     1561 gcccgtccga cccaagcgtc aatgcagcga gcggtcagcc gtgatcatgc tcgctatgct
     1621 gcttgcgaat acgtcgcacc gccatggcga cgccgaagat cacgaacggc agggccagcg
     1681 ccgtcagcac agaggaatcg agcttggtca gttcagcgtc cttggcgccc ttgaacaggt
     1741 aaccgagcaa tccgagcacg taatagctga cggccgccac cgacagacct tccacggtct
     1801 gctgcatcct cagctgcaac ctggcgcgct cattcattga ctgcatcgag tcgcggttct
     1861 gctgttcaag gtcgacctgg acgcgcgtgc gcagcaggtt ggcattgcgg gcgatcttct
     1921 cggcaagccg cgactggcgt tcggccacca tgtggcaggt gcgcagcgcc ggcttgacgc
     1981 gccgggcaag gaaggtcgcg aaggtcggat agccgagcgg cagcggtgtc tcacgcaggg
     2041 attccacccg ctggtcgatg atgccctcat aggcgcggac ggcggaaaag cggtaatcgg
     2101 tgcgcgagct ttccagttcc aggcttgagg ccagctgcat caatcgctca agctgggcgt
     2161 ggttgccatc aaagcctgtg gcctcggtca tggcgcgggc gatgtcggtt agctcctcct
     2221 ccgccttgcg cagggccggc gtcaccgcct gcgccgtcgg caggccgagc agggcaaaga
     2281 cgcgataggt ttcgatctcc agcaagcgct gggtcagccg tccggcggtg tcgggggtca
     2341 gattctggtt gatgatcagg aagcgcacga agccgtcgct ggtcaccttg aaatcggacg
     2401 ctgccgtggc aatgccaccg tcgatcgtcg aggcagccag actcatcgca tcgaacacgc
     2461 cttccagccc gccatcgggc atggtgtcga caaggtgcag atcgatcgcg ctcaaaagcg
     2521 gaccgggctg gggaatatcg cgcatcacct gcgccagttg tcccgccggc cgctcgaacg
     2581 gcgtccggtc ggtgccgccg atctcgaagg tataggttgt gaactcgctg tgctgctccc
     2641 agcgcaacac cgcgtcggac agggtcaccc gcaggtgctt cagcccctcg gccgggccct
     2701 gcacgccgcg cgactggcag aaagccgtca aagcgccgcg atccttcttc gattgctccc
     2761 aatcggtggt gaaggcaaag cgcagaatgc gactgggtgt cggcaccgga tagaacggcc
     2821 gggcgtggac ctcgccaagc acgggtccgc gctgcggatg gggggtaaac gtctgtgcgg
     2881 cggcggcggg tgccggtcgg acttcagcca cggaagcatc ctcaagaatg gcgaaacagc
     2941 ggcacgcatg acatgtgccg gcaaacctgc caagcatacg ccatgacgcg cgacaattca
     3001 cccggatcga ccggttgcgc aaggctgccc cgatgccttg cagattggtt ctcggcgcgc
     3061 ggctggcgcc tgcggcaaca tcaggccgac ctcatcgact tggcggccca gggccggtca
     3121 gcgctactga tcgcaccaac cggagcgggc aagaccctgg ccggattcct gcccagcctg
     3181 atcagcctgt ccgaacccgg caagcgccgg cggggcggat tgcataccct ctacgtctcg
     3241 ccgctgaagg cgctcgccgt cgatatcacc cgcaatctcg aagcgccggt cggcgaaacc
     3301 ggcctgccga tccgcatcga gacccgcacc ggagatacgc ccgccagccg ccgccagcgc
     3361 cagcggcgcg atccgcccga catgctgctg accacccccg aacagatcgc gctgatgctg
     3421 tcgggaagcg atgcggagca tctgttcgct gcgctggatc gcatcatcgt cgatgaggcg
     3481 catgcactgg cgccgatcaa gcgcggcgac cagcttgccc tgtgcctttc gcggctgcgg
     3541 cggctcaaac ccgatgtcac cgtcaccggg ctttcggcga cggtccgcga tcctgtcgcg
     3601 ctggcgcgct ggctggcgcc ggaggcaacc atcgtgacag ccgggcagga tgctgcggca
     3661 gcagccgttc tcgacattct cgactgcggc gcgccgctgc cctgggcggg ccatatcgcc
     3721 cgccatgcgg tggaggcgat ctattcggca atcggcggtc atcgactcag tctcatcttc
     3781 gtcaataccc gcatgcaggc cgaattcctg ttccaacgcc tgtgggagat caacgaggac
     3841 ggacggcgca tcgcgctgca tcacggatcg ctcgatgccg gccagcgccg caaggtcgag
     3901 gcggcgatgg cggccggccg gctcgatgcc gtggtcgcca cctcgacgct cgatctcggc
     3961 atcgactggg gcgatgtcga tctggtcatc aatgtcggcg caccaaaggg agccagccgg
     4021 ctgatccagc gcatcggccg cgccaatcac cggctggata cgccatcgaa ggcgctgctg
     4081 gtgccggcca accgctttga actcctggaa tgccgcgccg ccatcgccgc agcccgggcg
     4141 ggcacgcagg atgccgacgt catccgcacc ggcgggctcg acgtgctggc ccagcatgtg
     4201 ctcgcatccg ccgccgcagg gccgttcgac gcggaggccc tgttcgccga ggtgcgcagc
     4261 gcagcgccct atgccgatct gacgcgcgag gattacgatg ccatcgtcga cttcgtcgcc
     4321 acaggtggct atgcgctgaa aacctacgag cgcttcgcca agatcaggcg gacaagagat
     4381 ggcctctggc gtatcgccaa tcccgccgtc gcgcagacgt tccgcatgaa tgtcggcacg
     4441 atcgtcgagg aagcgatgct gaaggtgcgc ctcgggcgcg cccgctccac cggcagcggc
     4501 ccgaccgggc cgatcggccg tgccggacgg ctgctcggcg aggtggagga atatttcgcc
     4561 gaacagttgc agccgggcga taccttcgcg ttcggcggcg aaatcctcgc cttccaggcg
     4621 ctggtcgaca acgaggtgat cgtggcgcgg accagcgcca gggaccccaa ggtgccctcc
     4681 ttcatgggcg gcaagtttcc gctgtcgacg catctgtccg aacgggtgcg ggcgatgctg
     4741 gccgagccgg cgacatgggg cgattttccc gccgaagtgc ggaactggct tacacttcag
     4801 caagcggtct cgcgactgcc gggaagggct gatcttctgg tcgagacctt cccgcgcggc
     4861 ggacggttct acatggtcgc ccatgccttc gaggggcggc tcgcccatca gaccctcggc
     4921 atgctgctga cgcggcggct cgagcgggcg cgcctgcaac ccatgggctt tgtctgcaac
     4981 gattatgccc tgtgcatctg gggagcgcgg gatatcggcc tggccgccgc gcacgatccc
     5041 gacctcttcg gccatctgtt cgacgaggac atgatgggcg atgatctgga ggaatggctg
     5101 gccgaatcgt cgatcatgaa gcgaacgttc cgcaatgccg cgatcatcgc cgggatgatc
     5161 gagcggcgct ttcccggcgc cgaaaagggc cggcggcaac tgacgatgtc ctcggacctg
     5221 atctacgatg tgctgcgccg ccatcagccc gatcatatcc tcatcaaggc cgcgcgtgcc
     5281 gatgcggcca gtggcctcat cgacgcgcgg cggctgggcg aactgctggt tcgcatcgcc
     5341 ggacgaatcg tccacaagag gctcgaacgg gtctcgcctc tggcggtgcc ggtgatgctg
     5401 gaaatcggcc gcgagcaggt ctacggcgag gctgccgacc ggttgctcga cgaagcggcc
     5461 ctcgatcttg aacgggaggc gcttggtggc cttgccacaa ccgcgatcgg tggagcccgg
     5521 gcagatgccg gctgagatca tgctcggcgg cgccagcgtg cggctcgatg tctccggctg
     5581 cgcgatgctg atcggcgaag gggccctgct ggttgccgat ctgcaccttg aaaaaggctc
     5641 gtcctatgcc cgcggacgcc agttcctgcc tccctatgac acgcttgcca cgctggcacg
     5701 attgagcgag gtggtggcgc ggcttgcccc ccggcagatc gtggtgctgg gcgatgcctt
     5761 tcatgatcgc catgccggcg agcgcctgcc gcgtgaggcg gtggcggcca tcggggcgct
     5821 ggccaccggt cggcgcctgg tgtggatcgc cggcaatcat gaccctgccc cgcccgttgg
     5881 cttgccgggc gagcg
//
LOCUS       DAOMXG010000149         5885 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_7168_length_5885_cov_11.125043, whole genome shotgun sequence.
ACCESSION   DAOMXG010000149 DAOMXG010000000
VERSION     DAOMXG010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 5885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5885
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7168_length_5885_cov_11.125043"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(200..1105)
                     /locus_tag="PLQ11_09870"
     CDS             complement(200..1105)
                     /locus_tag="PLQ11_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016919460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HRK25247.1"
                     /translation="MREGLLPNNATHLLRLAAEETLARRAADLLTEILPAEETAAAAF
                     EVDDGRSWSLEVYFASPPDQSLIRELLAGIVGADLAETAEFAEITAKDWVSASLEGLA
                     PVAAGRFLIHGGHDRHRVRTNQCGIEIEAALAFGTGHHGTTRGCLLHLEDVLRQRRPH
                     RVLDVGTGTGVLAIAAARALKQPVSAGDIDPVSTITARANALANRAGAWLRPITAPGV
                     RHPELVREAPYDLIFANILARPLMRLAPTIASVASGRATLILSGLLPRDVNGILAAYR
                     AQGFRLVARKDLEGWVSLRLVCGSR"
     gene            complement(1148..1360)
                     /locus_tag="PLQ11_09875"
     CDS             complement(1148..1360)
                     /locus_tag="PLQ11_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HRK25248.1"
                     /translation="MLSGTVKWFNTQKGFGFIQPDAGGPDVFVHITAVQRSGLRELRE
                     GDKISYELVTDRRTGKTAAGNLQLAG"
     gene            1669..2565
                     /locus_tag="PLQ11_09880"
     CDS             1669..2565
                     /locus_tag="PLQ11_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008127574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRK25249.1"
                     /translation="MDPSRARIVGIGLMCLGISMFALNDAMGKWLLASYSVGQLLVVR
                     SVAALTVLTPAMLSEGRALLHPPHPGLQAVRVALSTVEVAMFYWAVSVMPLAETTTFW
                     LAVPIYVAVLAGPMLGERIPLRRWVAILVGFGGVVLAVNPGGAGLGWPVLIAFSGSLA
                     FAFMMIVTRHLRGTSDTTLVAWQTLAALIGGLALLPFEGWKPTGWRDLVLLSILGVIA
                     MLAHVATNRSLKLAPASVVVPYQYTIIVWAVTIGWLVFGDVPAMTTIIGALIIIAAGV
                     WIFWDEARAGQEALAETEEIKW"
     gene            complement(2597..3031)
                     /locus_tag="PLQ11_09885"
     CDS             complement(2597..3031)
                     /locus_tag="PLQ11_09885"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HRK25250.1"
                     /translation="MSNMMSKRGLLALGAALFVAPAAFAQQGGGAKPSLFINMTSDDS
                     HRLTMGLGFGLNQLKRGHSVTVFLNDRAVHAASKKNAEKFTEQQKIIADLLGAGATVL
                     VCPMCSKHYGVAEADFLPGLKLGNPELTGGALFADNARSLTW"
     gene            complement(3085..3474)
                     /locus_tag="PLQ11_09890"
     CDS             complement(3085..3474)
                     /locus_tag="PLQ11_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25251.1"
                     /translation="MLRVFRQMTGVDRLPIALAIVLAVLLRALVPAGYMPDVAALADG
                     RLALTICHGDELPKGDPTGGNPRPDQCPFAAISHAPLGASSERPCLAPAPAYFAFTVH
                     WGPVALTIPAHRPPLPFSARAPPPALA"
     gene            complement(3551..3832)
                     /locus_tag="PLQ11_09895"
     CDS             complement(3551..3832)
                     /locus_tag="PLQ11_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012496944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HRK25252.1"
                     /translation="MSRYIALIEDAGPDTAVGVWFPDLPGCFSAGDSIDEAMINAEEA
                     LALYAEATGKLPHPRTLTELKADRQIASDIASHIVALIALRNPVRSAAE"
     gene            complement(4272..>5885)
                     /locus_tag="PLQ11_09900"
     CDS             complement(4272..>5885)
                     /locus_tag="PLQ11_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRK25253.1"
                     /translation="PSPATRGEGGPHPANICCVGDDDQSIYGWRGADVDNILRFEHDF
                     PGSAVIRLERNYRSTGHILAAAAHIIANNEGRLGKTLFTDDAMGEKATVTGAWDSEEE
                     ARLICDEIEALQTKGHRLNEIAILVRASFQMREFEDRFVTTGVPYRVIGGPRFYERAE
                     IRDALAYLRLVNQPADDLAFERIYNQPKRGLGEAALATFNAYARSAGLPLLEAARQLA
                     ETEELKPKVRQTVRDLTQSFARWRANLETMKHTELAEMILEESGYTDMWKKDRSAEAA
                     GRLDNLKELVRSMEEFENLQGFLEHISLVMDAAEGDATDRVTIMTLHAAKGLEFETVF
                     LPGWEEGLFPHQRSLDENGRAGLEEERRLAYVGITRAKRRAKLYFATNRRMHGLWNAT
                     IPSRFIDELPEAHVEVVEAPGTGYGGYGNRGYGASRFDQMQPFGGSSYATPGWQRAAN
                     RATPGPEARGKTGWSGSGTAPRAPLMLEGELIAKSTGAPPSRFTPGTRVFHIKFGNGT
                     VTDIDGNKLTVDFDKAGRKMVLESFVQAG"
BASE COUNT         1019 a         1737 c         2005 g         1124 t
ORIGIN      
        1 agacatgctc taacttattg ataccgatca cgttatccgc attttgatga tttcatcaaa
       61 atgcggcgtg atctagagcc tgtcgcgatt aagtggatgc cggtcaatcg cagaagacat
      121 gctctaactt attgataccg atcacgttat ccgcattttg atgattccat caaaatgcgg
      181 cgtgatctaa gtcgacgttt cagcgactgc cacacaccag acgaaggctg acccagcctt
      241 cgagatcctt gcgtgcgaca aggcggaacc cttgcgcgcg ataggcggca aggatgccgt
      301 tgacgtcgcg cggcagcagc ccggacagga tcagcgtggc gcggccgctc gctaccgagg
      361 cgatggtcgg cgcaagccgc atcagcgggc gggcgagaat attggcgaag atcagatcgt
      421 aaggcgcttc gcgtaccagc tccggatggc gcacgcccgg cgcagtgatc ggccgcagcc
      481 aggctccggc gcgattggcc agagcatttg cgcgggcggt gattgtggag accgggtcga
      541 tgtcgccggc cgaaactggc tgcttcagtg cgcgcgctgc ggcaatcgcc agaacgccgg
      601 tgccggtgcc gacatccagc acgcgatgcg gcctgcgctg gcgcagaaca tcttccagat
      661 gcagcaggca gccgcgcgtg gtgccgtggt ggccggtgcc aaaggccagg gccgcttcga
      721 tttcgatgcc gcattgattg gtgcggacgc ggtgacgatc atgtccgcca tgaatgagaa
      781 aacgcccggc agcgaccggc gcgagccctt ccagcgaggc gctgacccaa tccttggcgg
      841 tgatttcagc aaattcggcg gtctcggcga gatcggcgcc gacaattcca gcgagcagct
      901 cgcgaatgag cgactgatcg ggcggggagg cgaaatagac ctccagcgac cagcttcgcc
      961 catcgtccac ctcgaaggca gcggcggcgg tttcttcggc cggcaggatt tcggtcagaa
     1021 gatcggcggc ccgtcgggcc agggtctcct ccgccgcgag gcggagaaga tgggtcgcat
     1081 tgttgggcag caatccttcg cgcatcggct cgcaataacc ggcagagtcc gatgcgtaaa
     1141 ggcttgatca gccggcgagc tgcagattgc cggcggcggt cttgccggtg cggcgatcgg
     1201 tgaccagctc gtagctgatc ttgtcgccct cgcgcagctc acgcaggccg gagcgctgca
     1261 cggcggtgat gtggacgaaa acgtccggtc cgccggcatc cggctgaatg aagccaaagc
     1321 ccttctgggt gttgaaccac ttaacagtgc cgctaagcat gggtctatcg tctttcctgt
     1381 tgcggccgag gccgcgtcgc gttggttcag agcggagata cgccccgtgg atggtcgaat
     1441 ttggggctag tctgaacggg cgccgacgga acccataggc aagtcgttat tccaggccaa
     1501 agtcgatggc caagcatacc acagtgctgc gctttgtcca tcggctctct tgaacgaccg
     1561 caggcgggag gggggcggat tgccaaaacc agcgggatcg gcaaagtatc gcgcccgggt
     1621 ctcggtgcga ctcggcttca cctccctcca gcatttttcc cggtcgacat ggatccttca
     1681 cgcgccagaa tcgttggcat cggcctgatg tgccttggca tatcgatgtt cgcgctcaat
     1741 gacgcgatgg gcaagtggct gctggcgagc tacagcgtcg gccagcttct ggtggtgcgt
     1801 tcggttgcgg cgctgacggt tctgacgccg gcgatgctga gtgagggcag ggcgctgctg
     1861 cacccgccgc atcctggcct gcaggcggtc cgcgtggcgc tctccaccgt cgaggtggcg
     1921 atgttctact gggcggtgag cgtcatgccg ctggcggaaa cgacgacctt ctggctggcg
     1981 gtgccgatct atgtggcggt tctggccggg ccgatgctgg gcgagcgcat cccgctgcgg
     2041 cgctgggtgg ccattctggt cggcttcggc ggcgtggtgc tggcggtcaa tccgggcggt
     2101 gcgggtctgg gttggccggt gctgatcgct ttctccggtt cgctggcctt cgccttcatg
     2161 atgatcgtga cacggcatct gcgcggcacg tccgatacga cgctggtggc ctggcagacg
     2221 ctggcggcgc tcatcggcgg gctggccctg ctgccgttcg aaggctggaa gccgacggga
     2281 tggcgtgacc tcgtgctgct gtcgatcctc ggcgtcatcg ccatgctggc gcatgtggcg
     2341 accaaccggt cgctgaaact ggcgccggcc agcgtggtgg tgccgtatca gtatacgatc
     2401 atcgtatggg cggtgacgat cggctggctg gtgtttggcg atgtcccggc gatgaccacc
     2461 atcatcggcg cgctgatcat cattgccgcc ggcgtgtgga tcttctggga cgaggcacgg
     2521 gcggggcagg aggcgctggc cgagacggag gagattaagt ggtagcgcgg ggggtgtccc
     2581 cccgcgcgcc ggatgctcac caggtcagcg agcgggcgtt gtcggcgaac agtgcgccgc
     2641 ccgtcagctc cgggttgccg agcttcagcc cgggcaggaa atcggcctcg gcgacaccgt
     2701 agtgcttcga gcacatcggg cagaccagaa cagttgcgcc cgcgccgagc agatcggcga
     2761 tgatcttctg ctgctcagtg aacttttccg cattcttctt cgatgcggca tggacggcgc
     2821 ggtcgttgag gaacacggtg acgctgtggc cacgcttgag ctggttgagg ccgaagccga
     2881 ggcccatcgt caaccggtgg ctgtcgtcgc tggtcatgtt aatgaacagc gagggcttgg
     2941 cgccgccgcc ctgctgggcg aaagcggcgg gcgccacgaa aagagcggcg ccaagtgcaa
     3001 gcaaaccgcg tttggacatc atgttggaca tggatctggt cctcgaaaga atgacattgc
     3061 ccgctaaagg ggcggatcgg tcgttcaggc gagggcgggc ggtggtgcgc gtgcgctaaa
     3121 cggcagcggt ggacgatggg ccggtattgt cagcgcgact gggccccagt gaacggtgaa
     3181 tgcaaagtaa gcaggggccg gcgccaggca gggccgttcg ctgctcgcgc caagcggcgc
     3241 atggctgatg gcagcgaagg ggcattggtc gggacgggga ttgccgccgg tggggtcgcc
     3301 cttcggcaat tcgtcgccgt ggcagatcgt gagtgccagc ctgccatcgg ccagcgcagc
     3361 gacatccggc atgtaaccgg ccggaaccag cgcgcgcagc agcaccgcca gcacgatcgc
     3421 cagcgcgatc ggcaatctgt ccaccccggt catctggcgg aaaacacgca gcactgtgca
     3481 ccccttgtgg cggcaggaat agcacgccaa accgggccgt tccagcgtgc tgcaatcggt
     3541 cgaaaggggg ctattccgct gccgaacgca ccggattgcg caaggcaatg agagcgacga
     3601 tgtggctggc gatgtccgaa gcgatctggc ggtctgcctt gagttcggtc agcgtgcgcg
     3661 gatgcggcag cttgccggtg gcttcggcgt agagtgccag cgcctcttca gcattgatca
     3721 tcgcctcgtc gatgctgtcg cctgccgaaa aacagccggg aaggtccgga aaccatacgc
     3781 caacggcggt gtccggtccg gcatcctcga tcagggcgat atagcgggac aacgttcaat
     3841 ccttgcgggg cttgaaggtc agcttcaact tgaatggatg taactccatt tcgagattgc
     3901 tgagccttct ttggttgacg agtagcacca gagtgacaac tgcgatcagc aaagcaatca
     3961 ccaagatggc agccattgtt agcatagtca tgtcctctag gctcatgcgt cacccttcca
     4021 atcagcaggt gtcgccttgc gacgtctcac cttataacga cagacgctca caaccgtgat
     4081 cgacgctgac gatcgtcagc agtagcgcat actaggttgc aggcggcccg tgcggggggt
     4141 ggacaggtgt ccaccccgct tgttgacgat gagattttcc ttgaggcaga gaaaaaagtt
     4201 cagggccagt ctgtgcctga tgaaaatttc aaaattgctc taaaattttc agcccgcttt
     4261 gattcgatgt ctcaccccgc ctgcacaaaa ctctccagca ccattttccg ccctgccttg
     4321 tcgaaatcga cggtgagctt gttgccgtcg atgtcggtca cggtgccgtt gccgaacttg
     4381 atgtggaaca cgcgcgttcc cggcgtgaag cgggaaggcg gggcgccggt ggatttggcg
     4441 atcagttcgc cttcgagcat caatggtgcg cggggggcag tgccgctgcc ggaccagccg
     4501 gtcttgccgc gcgcctcggg gccgggcgtg gcgcggttgg ctgcgcgctg ccatccgggt
     4561 gtcgcatagg atgagccgcc gaagggctgc atctgatcaa agcgcgaggc gccgtagccg
     4621 cgattgccat agccgccata gccggtgccg ggggcttcca caacctcgac atgcgcctcg
     4681 ggcaattcgt cgatgaagcg cgagggaatc gtggcgttcc acaggccgtg catgcgccgg
     4741 ttggtggcga aatagagctt ggcgcggcgc ttggcgcggg tgatgccgac ataggcgagg
     4801 cggcgttcct cctcgaggcc ggcgcggccg ttctcatcca gcgagcgctg gtgcggaaac
     4861 agcccttcct cccagccggg gaggaacacg gtttcgaatt cgagcccctt ggcggcgtgc
     4921 agggtcatga tggtgacgcg atcggtcgca tcgccctcgg cggcatccat caccaggctg
     4981 atatgttcaa gaaaaccttg cagattctcg aactcctcca tcgagcggac gagttccttg
     5041 aggttgtcga gccggccggc ggcctcggcc gaacggtcct tcttccacat gtcggtatag
     5101 ccggattcct ccaggatcat ctcggccagt tcggtgtgct tcatcgtttc cagattggca
     5161 cgccagcggg cgaagctctg ggtcaggtcg cgcacggtct ggcgcacctt gggtttcagt
     5221 tcttcggtct cggccagttg ccgcgccgct tccagcagcg gcaggccagc ggagcgggca
     5281 taggcgttga aggtggcgag ggcggcctcg cccaggccgc gcttgggctg gttgtagatg
     5341 cgctcgaagg ccagatcatc ggccggctgg ttgaccaggc gcagataggc cagcgcatcg
     5401 cggatttccg cccgctcgta gaagcgcggg ccgccgatga cacgataggg cacgccggtt
     5461 gtgacgaagc ggtcctcgaa ttcacgcatc tggaacgagg cgcgcaccag aatggcgatc
     5521 tcgttgaggc ggtggccctt ggtctgcaac gcctcgatct cgtcgcagat cagccgggcc
     5581 tcctcttcgg aatcccaggc gccggtgacc gtggctttct cgcccatcgc atcgtcggtg
     5641 aacagcgtct tgccgaggcg gccctcgttg ttggcgatga tatgggcggc agcggccagg
     5701 atgtggccgg tggagcggta attgcgttcg agccggatca ccgcgctgcc gggaaaatcg
     5761 tgctcgaacc tcaagatgtt gtcgacgtca gcgccgcgcc agccatagat cgactggtca
     5821 tcatcaccga cgcagcagat gttggcgggg tgggggcctc cctcgccccg cgttgcgggg
     5881 gaggg
//
LOCUS       DAOMXG010000150         5883 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_7174_length_5883_cov_11.130920, whole genome shotgun sequence.
ACCESSION   DAOMXG010000150 DAOMXG010000000
VERSION     DAOMXG010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 5883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5883
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7174_length_5883_cov_11.130920"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="PLQ11_09905"
     CDS             complement(<1..754)
                     /locus_tag="PLQ11_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007565227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRK25254.1"
                     /translation="MTPAPPLLSIDNLEVTFRTEAGAVTPVRGVSLTVQAGRTTALVG
                     ESGSGKSVTSLAVMGLLPRPAGSISGGAIRFRMKSGETSDLAALPPDAMRRLRGSEIA
                     MIFQEPMTSLNPVLTVGEQIAEQVRYHTGLNAVAAMRRAEEMLGLVEIPDAIRRATDY
                     PHQMSGGMRQRVMIALAMSCNPRLLIADEPTTALDVTIQAQILALIDRLRKQHDMGVL
                     FITHNLGVVAEVADDVTVMYAGEVVESADVRTL"
     gene            complement(754..1602)
                     /locus_tag="PLQ11_09910"
     CDS             complement(754..1602)
                     /locus_tag="PLQ11_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009489431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRK25255.1"
                     /translation="MDTTALRLFLKRLFRRKLVLFGALVLAAVVLLAIAAPWVAPYSP
                     TGMKVTQRLKPPSMVNWFGTDEFGRDVLSRIIYGGRASLGIGMAVVIASMLAGTTLGL
                     LAGYFRALDGIIMRIVDAMMSLPDILLAIFLVAVLGASAGNVVLALAIVYTPRVTRVV
                     RGSTLVVRELPFVEAAHVLGVSTWRILTVHILLNVASPILVQATFIFAYAVLAEAGLS
                     FLGAGVPPEIPTWGTMIATGQQYADQAVWVVLFPGLAIVLSALSLQLVGDGLRDMLDP
                     KLRKAL"
     gene            complement(1613..2554)
                     /locus_tag="PLQ11_09915"
     CDS             complement(1613..2554)
                     /locus_tag="PLQ11_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018448592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRK25256.1"
                     /translation="MFTAITRRLAGMLVVMFIVATMVFFLTRLAPGDPASLMLGDQAT
                     AADIAELRKTYGLDQPLLVQYVLWLKEVATGNLGQSIFLQRPVLQAIGERAEPTFFLA
                     LFSILIAALIGIPAGIASAVWRGRHADQTVSGLAMLAASIPSFWLGLLFIQFFAVRLG
                     WFPVAGYGDPGAGLAQRLYHLILPATVLGIVNSALITRFTRASMLDVLSEDYVRTARA
                     KGLPENRIVLRHALGNALVPIVTVVGLTAALLVGGAIITETVFGLPGIGNLVVSAVLR
                     RDYPVIQGALIVIAGIYVMINFAIDVIYTLVDPRVKT"
     gene            complement(2648..4219)
                     /locus_tag="PLQ11_09920"
     CDS             complement(2648..4219)
                     /locus_tag="PLQ11_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012653515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRK25257.1"
                     /translation="MSGLDKELSRSGWSRRDLAKFVAGAGLLATLPAGGKAFAQGAKS
                     ANLAMIGEPQSLDPMASTADLVSTIMMHVYESLYTFDKDLNAQPMLAAAMPKVSADGK
                     LYEIEIRKGVKLHSGRELDVEDVIASLNRWIAMTPRGKSVGANVDKLEAKGTHGLAIS
                     LKNPVPALLTHMALPSGYAAIMAKESIANPLVSFVGTGPYKFQERKPDQYVLLTKFDG
                     YSARTEASNGYAGKREAKIAELRFIPVPNANTRVEGVVAGQYHFADQLPVESYKRIAG
                     TASVKPVLVSPYGFPYFVLNTKQGPFADKKIRQAYQLALNNTEMMLAAFGDDKFFKAT
                     AAHFPKASPFHSEAGGAVYNKADAAAAKKLLAEAKYDGSPIRILNSKQYEFHNRMALV
                     MAENLKEAGFKVQLDVVDWATLVQRRADPAVYDVYVTHSTFLPEPMLSPPQLGDGAPG
                     WWETPTKKAALAAFNAESDPVKRGALWGKVQEIVYDEVPYIRVGDFAALSATSAKMTG
                     LVPMPSPAFWNVDLS"
     gene            4523..5665
                     /locus_tag="PLQ11_09925"
     CDS             4523..5665
                     /locus_tag="PLQ11_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020093978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HRK25258.1"
                     /translation="MNRTLILRGGRVIDPSQGLDGVMDIAFRDGRVTGIGAGLSAEAG
                     ARIIDVAGRIVTPGLIDLHTHVYDKGTSLGVDAMQLFRRGAVTTLVDTGSAGPGNFPG
                     FRAHVIERVPVRVLAYLNVSFAGIYGFSKRIMVGESGDMRLMAPIDTVEVAHANRDMI
                     IGIKVRVGRNASGFSGLAPLQIARQVADELGMPLMVHIDEPPPMLHDVLALMRRGDVL
                     THCFRPFPNCAATPDGKVHDYVLDARRRGVIFDIGHGMGSFSFRIARTMLANAFQPDC
                     ISSDVHALCIEGPAFDLLTTMSKFLCLDMPLVDVVCAATVAPARALSRPDLGTLAPGS
                     AGDASILSLETGAFDYVDSVGERLIGAQRLVAAGVVLAGQYHSCAA"
BASE COUNT         1101 a         1803 c         1925 g         1054 t
ORIGIN      
        1 acagggtgcg gacatcggcc gattccacca cctcgccggc atacatcacc gtgacgtcgt
       61 cggcgacctc ggcgacaacg ccgaggttgt gggtgatgaa cagcacgccc atgtcgtgct
      121 gcttgcgcag gcgatcgatc agggcgagaa tctgcgcctg gatggtgaca tcgagcgcgg
      181 tcgtcggctc atccgcgatc agaaggcgcg gattgcagga catcgcgagc gcgatcatca
      241 cgcgctggcg catgccgccg gacatctggt gcggatagtc ggtggcacgg cgaatagcgt
      301 cgggaatttc gacgagccca agcatctcct cggcccggcg catcgcggcg acggcgttca
      361 ggccggtgtg gtagcggacc tgttcggcga tctgttcgcc caccgtcagc accggattga
      421 gcgaggtcat cggctcctgg aaaatcatgg cgatctcgct gccgcgcagg cgacgcatcg
      481 cgtcgggcgg cagcgcggca aggtcgcttg tctcgccgga cttcatacgg aagcggatcg
      541 cgccgccgct gatgctaccg gcggggcgcg gcagcaggcc catcaccgca aggctggtga
      601 ccgacttgcc ggaccccgat tcccccacca gcgcggtggt gcgcccggcc tgcaccgtca
      661 ggctgacacc gcgcaccggc gtcacggcgc cggcttccgt ccggaaggtg acctcgaggt
      721 tgtcgatgga cagcaacggc ggtgcaggcg tcatcacagc gccttccgca atttgggatc
      781 gagcatgtcg cgcaagccat cgccgacgag ttgcaggctg agggccgaga ggacaatggc
      841 caggccgggg aacagcacca cccagacggc ctgatcggca tattgctgtc ccgtcgcgat
      901 catcgtgccc caggtcggga tttccggcgg cacgccggcg ccgaggaagg acaggccggc
      961 ttccgccagc accgcatagg cgaagatgaa ggtcgcctgc accagaatgg gtgaagcgac
     1021 gttgagaagg atatgcacgg tgaggatgcg ccaggtcgac acgccgagga catgggcggc
     1081 ctcgacgaag ggcagttcgc gcaccaccag cgtcgagccg cgcaccacac gggtgacgcg
     1141 cggcgtgtag acgatggcca gagccagcac gacattgccg gcggaagcgc cgagcacggc
     1201 gacgagaaag atcgccagca ggatatcggg cagcgacatc atcgcatcga cgatgcgcat
     1261 gatgatgccg tcgagcgcgc ggaaatagcc ggcgagaagg ccaagggtcg tgccggccag
     1321 catcgaggcg atgaccaccg ccatgccgat gccgagcgag gcgcgcccgc cgtagatgat
     1381 gcgcgagagg acatcgcggc cgaattcgtc ggtgccgaac cagttgacca tgctcggcgg
     1441 cttgaggcgc tgggtgacct tcatgccggt cggcgaatag ggcgcaaccc acggcgcggc
     1501 gattgccagg aggacgacgg cggcgagcac gagcgcaccg aacagcacca gcttgcgccg
     1561 gaatagccgt ttcagaaaca ggcgaagcgc ggtggtatcc atcgcggcgc cctcaggttt
     1621 tcacacgcgg atcgacgagc gtgtagatca cgtcgatggc gaaattgatc atgacataga
     1681 tgccggcgat gacgatcagc gcgccctgga tcaccgggta atcgcgccgg agcacggcgg
     1741 agacgacgag gttgccgatg ccgggcaggc cgaacaccgt ctcggtgatg atcgcgccgc
     1801 cgaccagcaa cgcggcagtg aggccgacca ccgtgacaat cggcaccagc gcgttgccca
     1861 gcgcgtggcg caggacaatg cggttctccg gcagtccctt ggcccgcgcg gtgcggacgt
     1921 aatcctcact cagcacatcc agcatgctgg cccgggtgaa gcgggtgatc agcgcggaat
     1981 tgacgatgcc aagcacggtc gccggcagga tgaggtgata gagccgctgg gcaagacccg
     2041 cgccgggatc gccatagccg gccaccggaa accagccgag gcgcacggcg aaaaactgga
     2101 tgaacaacag gcctagccag aaactcggga tgctggcggc cagcatcgcc agaccgctga
     2161 cggtctggtc cgcatgccgc ccgcgccaga cagccgaggc aatgccggcg ggaatgccga
     2221 tgagcgcggc gatcaggatc gagaacagcg cgagaaagaa ggtgggttcc gcccgctcgc
     2281 cgatggcctg cagcaccggg cgttgcagga agatcgactg gccaagattg ccggtcgcca
     2341 cttccttaag ccacagcaca tactgcacga ggagcggctg atcgagcccg taggtcttgc
     2401 gcaactcggc aatatcggcc gctgtcgcct gatcgccgag catcaacgaa gccggatcgc
     2461 ccggtgcaag gcgggtcagg aaaaacacca tggttgcgac gatgaacata acgaccagca
     2521 ttccggccag cctgcgggtg atagcagtga acatgcatcc tcccgattgt tcaggtgcgc
     2581 tacgatccgc tgcagcgaca ggcgctgcag cgttatctca aaaggtgccg acggtgattg
     2641 agtcgggtta cgacagatcg acgttccaga aggccggaga tggcatcggc acgagaccgg
     2701 tcatcttggc ggaggtggcg ctgagcgcag caaaatctcc gacgcggatg tagggaacct
     2761 cgtcgtagac gatttcctgc actttgcccc agagcgcacc gcgcttgacc ggatcgctct
     2821 cggcgttgaa cgccgccaat gctgccttct tggtcggtgt ttcccaccag cccggcgcac
     2881 cgtcgcccaa ctgcggcggg gagagcatcg gctcgggcag gaaggtcgag tgggtcacgt
     2941 agacatcgta aaccgccggg tccgcgcggc gctgcacgag ggtcgcccag tcaacgacgt
     3001 cgagctggac cttgaagccg gcctccttga ggttctcagc catcacgagt gccatgcggt
     3061 tgtggaactc atactgcttg gaattgagga tacggatggg cgagccatca tacttggcct
     3121 cggccagcag cttcttggcc gctgcggcgt ccgccttgtt gtagacggcg ccgccagctt
     3181 ccgagtggaa cggcgaagct ttgggaaaat gcgcggcggt cgccttgaag aacttgtcgt
     3241 cgccgaaggc ggccagcatc atctcggtgt tgttgagcgc gagctgatag gcctgacgaa
     3301 tttttttgtc agcgaaaggc ccctgcttgg tgttgagtac aaaatacggg aagccatagg
     3361 ggctcaccag cacgggcttc accgaggccg tgccggcgat gcgcttgtag ctttccaccg
     3421 gcagctggtc tgcgaagtga tactggccgg caaccacgcc ctctacgcgg gtgttggcgt
     3481 tcggcaccgg gatgaagcgc agctcggcaa tcttcgcctc acgctttccg gcatagccgt
     3541 tcgatgcttc ggtgcgggcg gagtaaccgt cgaacttggt caggaggaca tactgatccg
     3601 gcttgcgctc ctggaacttg taaggcccgg taccgacaaa gctgaccagc ggattggcga
     3661 tcgattcctt cgccatgatc gcggcatagc cggagggcaa cgccatgtgc gtgagcagcg
     3721 ccggcaccgg attcttgagc gagatagcca gaccatgggt gcccttggct tccagcttgt
     3781 caacattggc gccaacggat tttccgcgcg gggtcatcgc gatccagcgg ttgagcgagg
     3841 caatgacgtc ctcgacatcg agctcccggc cagagtggag cttcacgcct tttcggattt
     3901 cgatctcgta gagtttgcca tcggcactga ccttcggcat cgctgctgcc agcatcggct
     3961 gtgcgttcag gtccttgtcg aaggtgtaga gcgactcata gacatgcatc atgatggtcg
     4021 agacaaggtc ggcggtcgaa gccatcgggt ccagcgattg cggctcgccg atcatcgcca
     4081 gattggcact ctttgcgccc tgcgcgaagg ccttgccacc tgcaggcagg gtcgcaagaa
     4141 gcccggcgcc agcgacgaat tttgcaagat cgcgccggct ccagccgctg cgtgacaatt
     4201 ccttatccag acccgacatc gacacccctc ccatgagcgc gagccggacg cggctggcgc
     4261 ggttcctatt ctagaattga aactttattg cctcgccgcg aggtgtcaag cgccaaacgc
     4321 gcaccttgag gtcgccgcat tgcccgcttc agccatcaac acgcgcctat tgcattgcaa
     4381 tatgtcgcta ttgcgctgca gcgcgccatc gctttaaggc cccgctcccc aattatggaa
     4441 tgttattact tttttgattg atctgacacg ctcgttttgt atcctgaccg gacctgatcc
     4501 tggctgctcc ggaggcttgt ccatgaaccg caccctcatc ttgcgcggcg gtcgcgtgat
     4561 cgacccatcc caggggctgg acggggtcat ggatatcgcc ttccgcgatg gcagggttac
     4621 cgggatcggc gccggcctca gcgcggaggc cggagcaagg atcatcgatg tggccggcag
     4681 gatcgtgacg cctgggctga tcgacctgca cacccatgtc tatgacaagg ggacgtcgct
     4741 cggcgtcgac gccatgcagt tgttccggcg cggcgcggtg accaccctgg tcgataccgg
     4801 cagcgccggg cccggcaatt ttccggggtt ccgcgcccat gtgatcgagc gtgtcccggt
     4861 tcgtgtgctg gcctatctca atgtttcctt cgccggcatc tacgggtttt cgaagcggat
     4921 catggtcggt gaatccggcg atatgcggct tatggcgccg atcgatacgg tcgaggtcgc
     4981 ccatgccaac cgggacatga tcatcggcat caaggtgcgt gtcggacgca acgccagcgg
     5041 gttttcaggc cttgccccgc tgcagatcgc ccgccaggtg gccgacgaac tcggcatgcc
     5101 gctgatggtg catatcgacg agccgccgcc gatgctgcac gacgtcctgg ccctgatgcg
     5161 caggggcgac gtgctcaccc attgtttccg accgttcccg aattgcgcgg cgacgccgga
     5221 cggcaaggtt cacgactacg tgctcgatgc acgccggcgc ggcgtgattt tcgacatcgg
     5281 ccatggaatg gggtcgttct cgttccggat tgcccgcacc atgctggcga acgccttcca
     5341 gccggactgc atttccagcg atgtgcatgc gctttgcatc gaggggccgg ccttcgacct
     5401 gttgaccacg atgtcgaagt tcctgtgcct cgacatgccg ctagtggacg ttgtgtgcgc
     5461 ggcaacggtt gctccggcaa gagcgctcag ccgtcccgac ctcggcacgc tggcgccggg
     5521 gtcggcaggc gatgcctcga tcttgtcgct tgaaacgggc gcgttcgact atgtcgactc
     5581 ggttggagaa cgactgattg gcgcacagcg tcttgtcgca gccggcgttg tgcttgctgg
     5641 acagtatcat tcctgcgcgg cgtaagcgcg gcactcagag cgcatcagca ttaaaagaca
     5701 gccagtcact ctaacgagtg caatgcaaga gttgcgtctg gttataaagg atgggcaatc
     5761 gcaacctgcc attccggttt cggccccttt gccgtcattc ctgtccagtg tgagagttgg
     5821 gttttccggg gtctgaagcc cgggatgtga agagagagtt tccgggctgg ttctgaaccc
     5881 ctg
//
LOCUS       DAOMXG010000151         5880 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_7179_length_5880_cov_8.997082, whole genome shotgun sequence.
ACCESSION   DAOMXG010000151 DAOMXG010000000
VERSION     DAOMXG010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 5880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5880
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7179_length_5880_cov_8.997082"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1409)
                     /gene="fliF"
                     /locus_tag="PLQ11_09930"
     CDS             complement(<1..1409)
                     /gene="fliF"
                     /locus_tag="PLQ11_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019904645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HRK25259.1"
                     /translation="MQTILALVQKLGPQRLAAMGAVTLSLVGFFAFVMLRLSQPAMGL
                     LFTDLSPSESAAIIKELDGRGLRSELRNDGATVLVPRDNLARIRMDLAAKGLPAGGGI
                     GFEIFDKGDSFSATSFVQNINQVRALEGELSRSIRSLDRVQNARVHLAIPERRLFQKE
                     RAEPRASIVLKVRGELDAAQVRAIRHLVATAVDGLKPGRISLVDEAGRLLADGAGDEA
                     DVSAAALDRQQMHERRLQKQVEDIVASVVGRGRARVQVAADYELNRIQQTAETFDPES
                     RVVRSTQNRTETNSSTEGRDGQVTVANEQPGTQKSEAGGSPSREQGQKNEEVTNYEIS
                     RTTRTEVIEPGRLKRLSVAVLVDGLYSKGANGALTYAPRQAEELQRIATLVRLGIGFN
                     KERGDQVEVVNLPFAEAPQAADLAEPPLLERLLAFSRDDLMRLSEAGVLLLTTLIVLI
                     FVVRPLIRQVLAPPVTGRTA"
     gene            1541..2512
                     /gene="pdxA"
                     /locus_tag="PLQ11_09935"
     CDS             1541..2512
                     /gene="pdxA"
                     /locus_tag="PLQ11_09935"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003576232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HRK25260.1"
                     /translation="MQLPIAITMGDSAGIGPELVVRFFVERPGEWAVAIGDAGMLRRA
                     AALVGADLEVVEIERPEALTPARGRLPVLPVSHLPADLAYGRVDARAGRAAYDAIIAA
                     IDLALAGRIAAMVTAPIHKEALAAAGIAHPGHTEILAERTGARHFGMLLADRHLRVVL
                     VSIHVPLSQAIRLVSFDTVAAAIRLADQGARAFGIARPRIAVAGLNPHAGEGGLFGRE
                     EIDIIAPAVAAMRAEGLDVSGPFAPDTVFMRARQGEFDIVVAQYHDQGLIPVKYNGID
                     GGVNVTVGLPIIRTSVDHGTAFDLAGKGIASAASLLAAYDVARQMCT"
     gene            complement(2590..2865)
                     /locus_tag="PLQ11_09940"
     CDS             complement(2590..2865)
                     /locus_tag="PLQ11_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010659559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1153 domain-containing protein"
                     /protein_id="HRK25261.1"
                     /translation="MTEVYRPRAKYVIGPDGSPLTIADLPPTNTRRWVIRRKAEVVAA
                     VRGGLLSLDEACSRYTLTVEEFLSWQQSIDDHGLAGLRTTRIQHYRA"
     gene            complement(2957..3625)
                     /locus_tag="PLQ11_09945"
     CDS             complement(2957..3625)
                     /locus_tag="PLQ11_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HRK25262.1"
                     /translation="MALSSISGTSSSPALGGARSATLAENFQTFLGLLTTQLKNQSPL
                     DPLNTNEFTQQLVQFASVEQQMKSNETLTALLTATKVNTSTAALGYVGATITADGATA
                     RLSGGSARWQLQVPRSVSGATLTVTDSTGATVHSETRALAAGEQPFLWNGRMSSGAIA
                     PDGNYNLKIVARDAAGQPVTVRTEVQGIVESVDVTGPEPVLSVGNLTLPVSKVKTIRI
                     ARGT"
     gene            complement(3635..4522)
                     /locus_tag="PLQ11_09950"
     CDS             complement(3635..4522)
                     /locus_tag="PLQ11_09950"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HRK25263.1"
                     /translation="MPASEGVREDSAIATQLALPTTEFVSLLAEAAGQAPEGPAGMPS
                     IPGMPGKPDMAAAGTTLMIAGTTSAPPSTEPAQLAPPATVTGVSQVLPASTAAKDEPA
                     AATAVPSPAAPPQHPGAAELPAATPPLPPNPVQTPLTTQIQTSHHTPAMAPGRAVPPE
                     LVPITIASLASTGERRFDIRLDPADLGRIEVTLSVDAEGGVRTHLVVERPETLTLLRK
                     DLPRLEQALDATGLKSDPSGISLGLRNGGQGHDAHARPDAAPVNPAHSENPGSEQAPP
                     AYRQPRPLPGSAGRLDLIA"
     gene            4981..>5880
                     /gene="mnmA"
                     /locus_tag="PLQ11_09955"
     CDS             4981..>5880
                     /gene="mnmA"
                     /locus_tag="PLQ11_09955"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041799471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HRK25264.1"
                     /translation="MLNSLDIDKPVSATRVVVAMSGGIDSSVVAALLKREGYDVIGIT
                     LQLYDHGAAVHRVGACCAGQDIQDAKRVAESLGIPHYVLDYESRFRDSVIDAFAASYA
                     AGETPVPCIECNRTVKFRDLLQVARDLGADVLATGHYVESRRLADGHRAMFRPVDQER
                     DQSYFLFATTQEQLDFLRFPLGGMTKAETRALARDFGIAIADKPDSQDICFVPQGRYN
                     ALIEKLKPEAVRPGEIVHIDGRVLGRHEGIHNFTIGQRRGLGLATGEPLFVLRLEAAS
                     ATVIVGPRATTTPSSPLASRTWQP"
BASE COUNT          964 a         1775 c         2058 g         1083 t
ORIGIN      
        1 gcggttcggc cagtcacagg cggggcgagg acctgacgga taagcggccg caccacgaag
       61 atcagcacaa taagcgtcgt cagaagcagg acaccggcct cggacaaccg catcaggtca
      121 tcacggctga aggcgagcag ccgttcgagc agcggcggct cggccagatc ggcagcctgc
      181 ggcgcttcgg caaagggcag gttgaccacc tcgacctggt cgccccgctc cttgttgaag
      241 ccgataccaa gccgaacaag cgtggcgatg cgctgcaatt cctccgcctg acgcggggca
      301 taggtcaacg cgccgttggc gcccttgctg tagagcccgt cgaccagcac agccaccgac
      361 agccgtttca gccggcccgg ttcgatgacc tcggtgcggg tggtgcggga aatctcgtaa
      421 ttggtgacct cttcattctt ctgtccctgc tcgcgtgacg gagatccgcc ggcctcgctc
      481 ttctgggtgc cgggctgctc gttggcgaca gtgacctgcc catcgcggcc ctctgtcgaa
      541 ctgttcgttt ccgtgcggtt ctgggtcgag cggacgaccc ggctttcagg atcgaaggtt
      601 tcggcggtct gctggatgcg gttgagttcg tagtcggcag cgacctggac ccgtgcccgg
      661 ccgcgtccga cgacggaggc gacgatgtcc tcgacctgct tctgcagccg gcgctcatgc
      721 atctgctggc ggtcaagcgc agcggccgag acatcggcct cgtcgccggc gccgtcggcg
      781 agcaggcggc ctgcctcatc cacaagcgag atccggccgg gcttgaggcc atcgacggcg
      841 gtggcgacga gatggcggat ggcacgcacc tgcgctgcgt cgagttcgcc gcgcactttc
      901 agcacgatcg aggcgcgggg ctcggcccgt tccttctgaa acagcctgcg ctcgggaatg
      961 gccagatgca cccgcgcgtt ctgcacgcga tcaagcgagc ggattgaacg cgacaattct
     1021 ccttcaaggg cgcgaacctg gttgatgttc tggacaaaac tggtggccga gaacgaatcg
     1081 cccttgtcaa aaatctcaaa gccgatgccg ccgccggccg gcaggccctt ggcagccaga
     1141 tccatccgga tgcgcgcgag attgtcgcgc gggaccagca cggtggctcc gtcgttgcgc
     1201 agttcactgc gcagcccccg cccatcgagt tccttgatga tggcggctga ttcggatggc
     1261 gacaggtcag tgaacagcag gcccatggcc ggctgcgaca ggcggagcat gacaaaggcg
     1321 aaaaagccga ccaggctgag cgtaaccgcc cccatcgcgg ccaggcgctg ggggccaagt
     1381 ttctggacga gagcgagaat tgtctgcact gtgatgaacc cgggatcgct ccggccggat
     1441 acaattgccg gccactgggc aatttttgcc ggtgtgagcg ttagcgaaga gttaaaatat
     1501 gcaccctcag gttgaaaaaa agcagcgcgg agcgcgatca atgcaattac cgattgcgat
     1561 caccatgggc gattccgctg gaatcggtcc cgaactggtg gtgcgtttct ttgtcgagcg
     1621 gcccggcgaa tgggctgtgg ccatcggcga tgccggcatg ctgcggcggg ctgcggcact
     1681 ggtcggcgcg gaccttgagg tggtggagat tgaacgacct gaagcattga ccccggccag
     1741 ggggcgcctg ccggttctgc cggtctcgca tctgccggcg gatctggcct atggccgcgt
     1801 cgatgcgcgc gccgggcggg ctgcctatga tgcgatcatt gcggcgatcg atctggcgct
     1861 tgccgggcgg atcgcggcga tggtcacggc gccaattcac aaggaggcgc tcgccgcagc
     1921 cggtatcgcc catcccggac atactgaaat tcttgccgag aggaccggcg cccgacactt
     1981 cggcatgctg ctcgctgatc ggcatcttcg ggtcgtgctg gtgtctatcc atgtgccgct
     2041 gagccaggcc atccggctgg tctctttcga cactgtggcg gcggcgatcc ggctggcgga
     2101 tcagggagcg cgcgccttcg gcatcgcccg gccgcggatc gcggtcgccg gtctcaatcc
     2161 ccatgccgga gaaggcgggt tgtttggccg cgaggagatt gacatcattg ctcctgcggt
     2221 cgcagccatg cgcgcggagg gtctcgacgt ctcgggcccc ttcgcgccgg atacggtgtt
     2281 catgcgggcg cggcagggcg aattcgacat cgtggtggcg caataccacg atcaggggct
     2341 gattccggtc aaatacaacg gcattgatgg cggagtgaat gtcaccgtcg gcctgccgat
     2401 catccgcacc agcgtcgatc atggcacagc cttcgatctg gcagggaagg ggatcgcgtc
     2461 ggcggcaagc ctgttggcgg cctatgacgt ggcacggcag atgtgcacat gactacgcgc
     2521 cgcgcgaggg cggcggttca aatcggttcc ggcgctgaac agcgcccgga tgcgacgccc
     2581 ggacggggct caggcgcgat agtgctggat gcgcgtcgtg cgcaggccgg cgaggccgtg
     2641 atcatcaatg gattgctgcc aggacaggaa ttcctcgacg gtcagggtat agcggctgca
     2701 agcctcatcg agcgacagca atccgccacg cacggccgca acaacttccg ccttgcggcg
     2761 gatcacccag cggcgggtgt tggtcggcgg caggtccgca atggtcaggg gactgccgtc
     2821 cgggccgatg acatacttcg cacgcgggcg atagacttcg gtcatggggt acgctcacat
     2881 gaactacgca acttgagatg tgaggttaga ccggggtcgc ttaaaaattg cctaagcccc
     2941 gatgaaacat tgcagattat gttcccctgg cgattcggat tgtcttaacc ttgctgaccg
     3001 gcagagtgag gtttccgacg ctcagaaccg gttccgggcc ggttacatcg acgctttcga
     3061 cgattccctg cacttcggtg cggaccgtca ccggctggcc ggccgcatcg cgggccacga
     3121 tcttaaggtt gtagttacca tccggagcaa tcgcgccgct gctcatgcgc ccgttccaga
     3181 gaaaaggttg ttctcctgcg gcaagggccc gcgtttcgct gtggacggtt gcgccggttg
     3241 aatcggtcac cgttagggtc gctcccgata ccgagcgcgg aacctgaagc tgccagcggg
     3301 cgctgccgcc ggatagccgg gcggtcgcac cgtcggcggt gatggtggcg ccaacatagc
     3361 cgagcgctgc cgtcgaggtg ttgaccttcg tggccgtgag cagcgcggtc agcgtctcgt
     3421 tggatttcat ctgctgttct accgaggcaa actggaccag ctgctgggtg aattcgttgg
     3481 tgttgagcgg atcgagcgga ctctggttct tgagctgggt ggtcagcaat ccaagaaacg
     3541 tctggaagtt ctcggcgaga gtcgcagagc gagcacctcc gagggcgggg gacgatgacg
     3601 tgccggaaat ggaggacagg gccatggcgg ttcctcaggc gatcaggtcg agacggccgg
     3661 ccgaacccgg caacgggcga ggctggcgat aggcaggcgg ggcttgttcc gagcctggat
     3721 tttcggaatg ggcgggattg accggggcgg cgtcaggccg ggcatgcgca tcgtgcccct
     3781 gtccgccatt cctgagacca aggctgatgc cggatggatc agatttcagc cctgtcgcat
     3841 cgagggcctg ttcgaggcgg ggcagatcct tgcgcagcag cgtcagcgtc tcggggcgtt
     3901 cgacaacgag atgggtgcgc acgccgcctt cggcatcgac cgacagcgtg acctcgatac
     3961 gcccgagatc agccggatcg agtcggatgt cgaagcgccg ttcgccggtg ctggcgaggc
     4021 tggcgatggt gatcggcacc agctcggggg gcacggcgcg gcctggcgcc atggcagggg
     4081 tatggtgcga ggtctggatc tgggtcgtca gcggcgtctg gaccgggttg ggcggcagtg
     4141 gcggtgtggc cgccggcagt tcggccgcgc cggggtgttg tggtggtgcg gcaggcgatg
     4201 gaaccgctgt cgctgcggct ggctcatcct ttgcggcggt ggaggctggc agaacttggg
     4261 aaacgcctgt taccgtggcc ggaggtgcga gctgggccgg ttcggtggat ggaggcgccg
     4321 atgtcgtccc tgcgatcatc aatgtcgtcc ctgccgccgc catgtccggt ttgcccggca
     4381 tgcccggtat gcttggcatg ccggctggac cctccggtgc ctggccagcc gcctcggcca
     4441 gcaggctgac aaattccgtg gtcggcaggg ccagttgggt agcgatcgca ctgtcctccc
     4501 gcacgccttc cgacgccggc atttcggatt ccgccgtctt gtcggctggt tccgggctgg
     4561 agggcggccg tccgtgatcg ccctggcgcg ggtggtcgtt gcggatacct ggccgaagct
     4621 gcccggtgaa ggatggtgga tcctcggctg ggtcgcgcaa ggcggggatt gcggcaaccg
     4681 gtgacgaagg ccggacgccg ggtgccgggg cgggagcgga gcgaggcagg gcagggatgg
     4741 gtgcgggcaa catgacgaat atcctcgacc gccagccctg caatcggtgg gccaacggcg
     4801 aggggtgcga aatcccatgc ttttcagcgc tctgactggg gttttcgggc tttcttcatt
     4861 cccgggcggc aagaattgcc tggtgtgcgg cctttcctgc cgcatcgcgc cgcgactggc
     4921 caagagagca aaaaactctt aatgaggcgc ggcccgccgt ttgtgttacc gtctcgcacc
     4981 atgctcaact cactcgatat cgacaagcct gtctctgcaa cccgcgtggt cgttgcgatg
     5041 tctggcggca tcgattcctc ggtggtggcg gcgctgctca agcgcgaggg ctatgatgtg
     5101 atcggcatca cgctccagct ctacgatcat ggcgcggcgg tgcatcgggt cggggcctgt
     5161 tgtgccggtc aggacatcca ggatgccaag cgtgtcgccg aaagcctcgg cattccccat
     5221 tatgtgctcg actacgagag ccggtttcgc gattcggtga tcgatgcctt tgccgcctcc
     5281 tatgcggccg gcgagacacc ggtgccgtgc atcgaatgca atcgcacggt gaagttccgc
     5341 gatctgttgc aggtcgcgcg cgacctcggc gccgatgtgc tggccaccgg ccattatgtc
     5401 gaaagccggc ggttggccga tggccaccgg gcgatgttcc ggccggtcga tcaggagcgc
     5461 gaccagagct atttcctgtt cgcaacgaca caggagcagc ttgatttcct gcgctttccg
     5521 ctgggcggaa tgaccaaggc cgaaacacgc gcgctggccc gggacttcgg catcgcgatt
     5581 gcagacaagc cggacagcca ggacatctgc ttcgtgccgc aggggcgcta taacgcgctg
     5641 atcgagaagc tgaagcccga agcggtgcgc ccgggcgaga tcgtgcatat cgatggccgg
     5701 gtgctcggcc gacacgaggg gatccacaat ttcaccatcg gccagcggcg agggctgggg
     5761 ctcgccacgg gtgagccgct gttcgtgctg cggcttgagg cggcgagcgc gacggtgatc
     5821 gtcgggccgc gcgcgacgac gacgcccagt tcgccgctgg ccagccggac gtggcagccc
//
LOCUS       DAOMXG010000152         5805 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_7315_length_5805_cov_8.073043, whole genome shotgun sequence.
ACCESSION   DAOMXG010000152 DAOMXG010000000
VERSION     DAOMXG010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 5805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5805
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7315_length_5805_cov_8.073043"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2086)
                     /locus_tag="PLQ11_09960"
     CDS             complement(<1..2086)
                     /locus_tag="PLQ11_09960"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HRK25265.1"
                     /translation="MRIERRYTKAGTSPYAGIAFHTTTSEIRNPDGSIVFRLEGLEVP
                     ETWSQVAADVLAQKYFRKAGVPKALKKVEENDVPSFLWRGVADEAALAGLPEKERTLS
                     ETSARQVFDRLAGCWTYWGWKGGYFTTEEDASAFFDEMRFMLATQKCAPNSPQWFNTG
                     LHWAYGIDGPGQGHYYVDYRTGKLTKSKSSYEHPQPHACFIQSVADDLVNEGGIMDLW
                     VREARLFKYGSGTGSNFSRLRGEGEKLSGGGRSSGLMSFLKIGDRAAGAIKSGGTTRR
                     AAKMVVVDVDHPDIEAYIDWKVKEEEKVAALVAGSKTLARHLKAILKACVNCEGEGDA
                     CFNPEKNPALKREIRAARKSHVPDGSIKRVIQFARQGFKDLAVDVYDTDWDSEAYLTV
                     SGQNSNNSVRVDDAFLNAVEAGADWQLKARMSGKVTKEIKARDLWDKIGYAAWACADP
                     GIQFHTTINDWHTCPASGSIVASNPCSEYMFLDDTACNLASLNLLEFYDPKSRVFDID
                     AYEHGVRLWTVVLEISVMMAQFPSKEIAQLSYDFRTLGLGYANIGGLLMTMGLPYDSA
                     EARALCGALTAIMTGICYATSAEMAGELGPFPSYPKNRNHMLRVIRNHARAARGETSG
                     YEALSTAPVALDHASCPVQELPARAVTAWEKALDLGQRHGYRNAQVSVIAPTGTIGLV
                     MDCDTTGIEPDFA"
     gene            2733..3140
                     /locus_tag="PLQ11_09965"
     CDS             2733..3140
                     /locus_tag="PLQ11_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010257 - NADH dehydrogenase complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase subunit NDUFA12"
                     /protein_id="HRK25266.1"
                     /translation="MEFLKQIFTWWNSQTIGTRLLTWRKGERVGEDQFGNVYYRTRGG
                     VKDKALGFERRWVIYNGEVEGSRVPPGWSGWLQHRTDVPPTKDDYKPREWELPHVPNM
                     TGTAAAYRPAGSTLSTGKRPPATGDYTPWTPGS"
     gene            complement(3149..3982)
                     /locus_tag="PLQ11_09970"
     CDS             complement(3149..3982)
                     /locus_tag="PLQ11_09970"
                     /inference="COORDINATES: protein motif:HMM:NF013918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HRK25267.1"
                     /translation="MPKPWQDRSGDLSPEKSIALALAILPALWIATQAALGWLAPRVL
                     ANATHQAGDWAIRLLWLTLTVTPLRRILDWPRLILARRILGLASFAYALLHVGLYVAN
                     LRFNLPLALSEIVQRIYLGIGLVGVIGLAILAAHSGDRAIQRLGSEAWRHLHRSIYLI
                     AIVALTHFFIQSRLDVTEPLVLTGLYLWLMGWRWLDSRRLSHAAGLAALAIASGLCTL
                     LIEAAWYAGVNAAPVLDVILANVDFAFEIRPMWIVLAATLSAAAAQRLRGRPPRSAAA
                     S"
     gene            complement(4220..4555)
                     /locus_tag="PLQ11_09975"
     CDS             complement(4220..4555)
                     /locus_tag="PLQ11_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009469317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HRK25268.1"
                     /translation="MFKNLDDLQKLGKDNADLLNKAFASQQKSAQAVAAEVQDYAKKS
                     MEAGSAFVEKLFGAKSLDKAIEVQTEYAKSAYEGFVASATKIGEIYADAAKEVAKPFE
                     TAFAKVQGK"
     gene            4856..>5805
                     /locus_tag="PLQ11_09980"
     CDS             4856..>5805
                     /locus_tag="PLQ11_09980"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003600921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HRK25269.1"
                     /translation="MRLSSVVRQFGRVAVLSMLASSVLVSLASQAEARRRAKAGYSPP
                     YASIVVDAKTGKVLQATNPDAPRHPASVTKVMTLYMLFEAMERGRFSLDSELKISAHA
                     ARQAPSKLGLQPGETISVEDAIKAVVTKSANDVAAAIGENVGGSEERFAELMTEKARA
                     LGMRGTTFINASGLPDREQITTARDLALLGRAIQDRFPKYYHFFGTRVFNYDGYAYRN
                     HNKLLGRVEGVDGIKTGYTRASGFNLLTSAKADGRHVITVVLGGKSGRVRDQQVAALV
                     DEHLPRAYAGARQIPRIVEVASAETEDRPAARSQQVAQAAP"
BASE COUNT         1085 a         1773 c         1897 g         1050 t
ORIGIN      
        1 gcgcgaaatc cggctcgatc ccggtggtgt cgcaatccat caccagaccg atcgtgcccg
       61 tcggcgcgat caccgacacc tgtgcgttgc ggtagccatg acgctggccg aggtccagcg
      121 ccttctccca ggcggtgacc gcgcgggccg gcagctcctg caccgggcag gaggcatggt
      181 cgagcgccac cggcgcggtc gacagcgcct cgtaaccgga agtctcgccg cgggcggcgc
      241 gggcatggtt gcggatgacc cgcagcatgt ggttgcggtt cttcggatag gacgggaagg
      301 ggccgagttc gcccgccatc tccgccgaag tcgcatagca gatgccggtc atgatcgcgg
      361 tcagcgcgcc acacagcgcc cgcgcctcgg ccgaatcata gggcaggccc atggtcatca
      421 acaggccgcc gatattggca tagccgaggc caagcgtgcg gaagtcatag gaaagctggg
      481 cgatttcctt ggacgggaac tgcgccatca tcaccgagat ttccagcacc acggtccaca
      541 gccggacgcc gtgctcatag gcgtcaatat cgaaaacccg gctcttggga tcgtagaatt
      601 ccagcaggtt gagcgaggcg aggttgcagg cggtgtcgtc caggaacatg tattccgagc
      661 acggattgga cgcgacgatc gagcccgacg ccgggcaggt atgccagtcg ttgatcgtgg
      721 tgtggaactg gatgcccgga tcggcacagg cccaggcggc atagccgatc ttgtcccaca
      781 gatcgcgcgc cttgatctcc ttggtcacct tgcccgacat gcgggccttg agctgccagt
      841 ccgcccccgc ctcgacggca ttgagaaagg cgtcgtcaac gcgcacggaa ttgttcgaat
      901 tctggccgga aaccgtcaga taggcttccg aatcccaatc ggtgtcgtaa acgtcgacgg
      961 cgaggtcctt gaagccctgg cgggcgaact gaatgacccg cttgatcgac ccgtccggca
     1021 catggctctt gcgggcggcg cggatctcgc gcttcagcgc cgggttcttc tccgggttga
     1081 agcaggcatc gccctcgccc tcgcaattga cgcaggcctt caggatggct ttgaggtgcc
     1141 gcgccagcgt cttggagccg gcaacaaggg cggcaacctt ctcctcttcc ttcaccttcc
     1201 agtcgatgta ggcctcgatg tcgggatggt cgacatcgac caccaccatc ttggcggcgc
     1261 ggcgcgtggt gccgcccgac ttgatcgccc ctgcggcgcg atcgccgatc ttgaggaagc
     1321 tcatcagccc ggacgaacgt ccgccgcccg acagcttttc gccctcgccg cgcaggcggg
     1381 agaagttgga gccggtgccc gagccgtatt tgaacagccg cgcctcgcgc acccacagat
     1441 ccatgatgcc gccctcgttg acgagatcat cggcgaccga ctggatgaag caggcatgcg
     1501 gctggggatg ctcgtaggac gatttcgact tggtcagctt gccggtgcgg tagtcgacat
     1561 aataatggcc ctggccgggg ccatcgatgc cataggccca gtgcaggccg gtattgaacc
     1621 actgcggcga attgggcgcg catttttgcg tggccagcat gaagcgcatt tcatcgaaga
     1681 aggccgaggc atcctcctcg gtggtgaagt agccgccttt ccagccccag taggtccagc
     1741 agccggcaag ccggtcaaac acctggcggg ccgaggtttc ggaaagggtg cgctccttct
     1801 cgggcaggcc ggcaagcgct gcctcgtccg caacgccgcg ccagaggaac gaggggacgt
     1861 cgttttcctc gaccttcttc aacgccttcg gcacaccggc cttgcggaaa tacttctgcg
     1921 ccagcacatc ggcggcgacc tgcgaccagg tttcggggac ttcaagccct tccagccgga
     1981 acacgatcga gccatccgga ttgcggatct cggaggtggt ggtgtggaag gcgatccccg
     2041 cataaggaga ggttcccgcc ttggtataac gccgttcgat ccgcattggt catcctttta
     2101 gtccgggcag aatcaacgcc cgtggccgcg tgacggcagc ccccacccat ttcaaactcg
     2161 gtttgacgcc cctgtcgcgc tggtgccctt gacaggcacg acaggccaga atgcccgctg
     2221 gcggcagggg atacccccca aaacacccaa ccggctgcgt ctggcccatt gctggcgcaa
     2281 cgagggatga cgataggtgg cgattccgct tcccgtcaat atatagttct tgcttgacag
     2341 gttccgcaca acatcttgtg gagcgctgtg aagattgggg acaatctcag catcgcgctg
     2401 caaggctctg gaatcgcggc ggcttttccg agaatcgaag agcgcggagc aacatcggga
     2461 ggcgctccgg catttaccgg gcgttaacca tcacggcaat cggtccggtt ccgggacaca
     2521 agatatagta ggccgcctag cccggcgccc ccaaaagccg gatgcatcgg gggctgcgga
     2581 cggttcccaa ggcgactcaa ctgtggccac ggaccggcgc gctcgccaat ggaatgaggc
     2641 aaaattgctg gacaggcggg cgtgcctgag tcaagacagg cgccgtgcct gccgtccgtg
     2701 gtccgggcgg ttccaactct ggggccgaag cgatggaatt tctcaagcag atcttcacct
     2761 ggtggaacag ccagacgatc ggaacgcggc tgctgacctg gcgcaagggc gagcgcgtcg
     2821 gcgaggatca gttcggcaat gtctattatc gcacgcgcgg cggggtgaag gacaaggcgc
     2881 tcggcttcga gcggcgctgg gttatctata acggtgaggt cgaaggctcg cgcgtgccgc
     2941 cgggctggtc cggctggttg cagcatcgca ccgatgttcc gccgaccaag gacgattaca
     3001 agccgcgcga atgggaattg ccgcatgtgc cgaacatgac cggcaccgcc gctgcctatc
     3061 gcccggccgg gtcgacgctt tcgaccggca agcgcccgcc agcgacgggc gattataccc
     3121 cctggacgcc gggaagctga acggccgatc aggacgcagc ggccgaacgc ggcgggcggc
     3181 cacgcaacct ctgcgcggct gcggctgaaa gcgttgctgc aagaacgatc cacattggtc
     3241 tgatttcaaa cgcaaagtcg acgtttgcta aaatcacgtc gagaaccggc gcggcgttga
     3301 cgccggcata ccatgccgct tcgatcagca gggtgcagag cccactggcg atcgccaggg
     3361 ccgcaaggcc ggcagcgtgg ctcagccggc gcgagtccag ccagcgccat cccatcagcc
     3421 agagatagag cccggtcagc acgagcggtt cggtgacatc cagtcgcgac tggatgaaga
     3481 agtgcgtcaa ggcaacaatg gcaatgagat agatgcttct gtgcaagtga cgccaggcct
     3541 cggatccgag ccgctggatg gctcggtcac cggaatgggc ggcaagaatc gccaggccga
     3601 ttactccgac aaggccaatg ccgaggtaga tccgctgcac gatctccgac agcgccagcg
     3661 gcaggttgaa gcgcagattg gcgacataca ggccaacatg cagcagtgca taggcgaaac
     3721 tcgccagtcc caggatgcgg cgggcgagaa tcaggcgcgg ccagtcgaga atgcggcgga
     3781 gcggcgtgac cgtcagcgtc agccagagca ggcggatggc ccagtcgccg gcctgatgcg
     3841 ttgcattggc gagaacccgc ggcgccagcc agccgagcgc ggcctgggtc gcaatccaca
     3901 gggcagggag gatggcgagc gccagcgcga tgcttttttc cggtgaaagg tcgccgctgc
     3961 gatcctgcca gggcttcggc atggtcttcc cgatcggttg gttgcctgcg catagcaagg
     4021 cgcgaccaca cgggccagcg gctgcggcct cacatggtgg tgatcaggac ggggcgtctg
     4081 cgtgatgagt tcggcccctg tcgccggatg cagggagggg cggtgccgac ttttgcgccg
     4141 cacaaaagaa aaggcccggc gggaggaacc gggcctttcc atcattgcaa ggtcggcgag
     4201 gagcgccaaa aacggcagct tacttgccct ggaccttggc aaaagcggtc tcgaacggct
     4261 tggcgacttc cttggccgca tcggcgtaga tctcgccgat cttggtggcc gaggcaacga
     4321 agccctcgta ggccgacttg gcatactcgg tctgaacttc aatagccttg tccaaagact
     4381 tggcaccgaa cagcttttcg acgaaagccg aaccagcttc catggacttc ttggcatagt
     4441 cctggacttc ggcggcgacg gcctgagcgg acttctgctg cgaagcaaaa gccttgttga
     4501 gcaggtcagc gttgtccttg ccgagcttct ggaggtcgtc gaggttcttg aacatggtag
     4561 gctccttgga ggggaagtgt gtctgcgttg aatttcgttg ccgtgaatat attgcaccgc
     4621 acaaattgtc aattcgtttt ttgcaatgca gcaatgcgcc ttccgtaggg tgcgccgtta
     4681 actgtagatt gatgcggtgg gcggaccggg cgttttctgc cgggcgctta agcttgaggt
     4741 aactcaaccc gtttaatttc gatcgaatgt gcgcaccggt gcttaaccca agccggcgcg
     4801 agcatgggtg gccggttgtt ccggtgcgta gaacggggcg cgttgcatga ttggtatgcg
     4861 tttgagtagc gtggtaaggc agttcggtcg cgttgccgtg ctgtcgatgc tggcctcgtc
     4921 ggtgctggtc tcgctggcca gtcaggccga ggcgcgcagg cgtgccaagg ccggatacag
     4981 cccgccctat gcgtcgatcg tggtcgatgc caagaccggc aaggtgttgc aggcgaccaa
     5041 tcccgatgcg ccgcgtcatc cggcctcggt caccaaggtg atgaccctct acatgctgtt
     5101 cgaggcgatg gagcgggggc ggttctcgct cgacagcgag ttgaagattt ccgcccatgc
     5161 cgcgcgccag gcgccgtcca agctgggctt gcaaccgggc gagacgattt cggtcgagga
     5221 tgccatcaag gccgtcgtga ccaaatcagc caatgatgtt gccgccgcca tcggcgagaa
     5281 tgtcggcggt tccgaggaac ggtttgccga attgatgacc gaaaaggcgc gtgcgctggg
     5341 catgcgtggc accacgttca tcaatgcgtc cggtctgccc gatcgcgaac agatcaccac
     5401 ggcgcgtgac ctggcgctgc tcggccgcgc cattcaggat cgtttcccga aatattacca
     5461 cttcttcggc acgcgggttt tcaactacga cggctatgcc tatcgcaacc acaacaagct
     5521 gctcggccgt gtggaagggg tggatggcat caagaccggc tatacccgtg cctccggctt
     5581 caatctgctg acctcggcca aggctgacgg tcgccatgtg atcacggtcg tcctgggcgg
     5641 caaatcgggg cgcgtgcgcg atcagcaggt cgccgcgctg gtcgatgagc atctgccgcg
     5701 tgcctatgcc ggcgcccgcc agattccgcg tatcgtcgag gttgccagcg ccgagaccga
     5761 ggacaggccc gctgcgcgca gccagcaggt cgcacaggcc gcacc
//
LOCUS       DAOMXG010000153         5753 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_7404_length_5753_cov_10.563531, whole genome shotgun sequence.
ACCESSION   DAOMXG010000153 DAOMXG010000000
VERSION     DAOMXG010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 5753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5753
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7404_length_5753_cov_10.563531"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="PLQ11_09985"
     CDS             complement(<1..869)
                     /locus_tag="PLQ11_09985"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRK25270.1"
                     /translation="MTTAAAGPALKQRAAPGGSRPLRLALIGYGLLLTAPLAGLAALA
                     FGPAAGTHLGWNAGLAATLQETLLLLAGVGILSAAIGVGAAWLIASYRFPGREVLAVM
                     LVLPLALPTYLAAYVAVELTDYFGPVQRMLRWALGVSSRSDYWFFEVRTLPGAIIILS
                     LVLYPYIYLPARLMFERQVGRIIYAARLHGAVGARLFFRIALPLARPAVAAGTAFALL
                     ETLNDVGAVEHLGVQSLSLTVRSLWLNRGNLPGAAQVALGCLVLVGGILIAEYALRRH
                     ASYASSARAGAPLT"
     gene            complement(866..1888)
                     /locus_tag="PLQ11_09990"
     CDS             complement(866..1888)
                     /locus_tag="PLQ11_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRK25271.1"
                     /translation="MRQTMTGRAMVLAGMMMASPAVAQEVNVYTYREPGLIKPIFDAF
                     TADTGIRVNVVFAKEGLEERIASEGARSPADALITVDVARLVRAVELGITRPVKSAEL
                     EAAVPQALRDRDGHWFGLSYRARVAYVSKERVKDAGLTYEDLAAPRWKGRFCLRDGQH
                     IYNNMLFAAGIAHMGADKAETWLKGLKANLAKKPSGGDREVAKDIAAGLCDIGLGNTY
                     YVGLMLQNPDQKPWAEAIRVILPKFSGGGTHVNLSGIALTRSGPNPASAEKLAAYLTG
                     DKAQRIYAESNFEFPVRNGTAIHPAVANFGALAPDEVPFAEIIRQRSRAADMIDRIGF
                     NGGPGG"
     gene            2130..3302
                     /gene="pobA"
                     /locus_tag="PLQ11_09995"
     CDS             2130..3302
                     /gene="pobA"
                     /locus_tag="PLQ11_09995"
                     /EC_number="1.14.13.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006468976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018659 - 4-hydroxybenzoate
                     3-monooxygenase activity [Evidence IEA];
                     GO_process: GO:0018874 - benzoate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HRK25272.1"
                     /translation="MRTRVAIIGSGPSGLLLGRLLSQAGIDNILIDTKSPEYILGRIR
                     AGVLEQGAVNLLEEAGAGARMRREGLVHDGFLISFDGQTHRMDLSRHSGGLSVMVYGQ
                     TELTRDLMEARKADAAPSLYEAEAVAIHDFDRSRPRVTLRHGGKPVTINADFIAGCDG
                     FHGVCRASVPAGAITGFERVYPFGWLGLLADVPPVHEELIYAHHERGFALCSQRSRSR
                     SRYYVQCSLDDTVDAWSDAMFWEELRRRLPAEVAARVKTGPSIEKSIAPLRSFVAEPL
                     RFGTLFLAGDAGHIVPPTGAKGLNLAASDIRYLFDGLVDFYARGSTTGLDSYSQRALA
                     RIWKAERFSWWMTNLLHSFPHASGFERRMQRTEFDYLISSEAAQRALAENYVGLPY"
     gene            3418..3699
                     /locus_tag="PLQ11_10000"
     CDS             3418..3699
                     /locus_tag="PLQ11_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25273.1"
                     /translation="MKSNIGKASADAIAKIAAGAGLAASAKAKAQGHAVTGTRMVEID
                     GKQKLCVVRIYPSGDMAVVKVLDPATPQTVRTSRRSSKRRAPAEAGQTK"
     gene            3724..4365
                     /locus_tag="PLQ11_10005"
     CDS             3724..4365
                     /locus_tag="PLQ11_10005"
                     /inference="COORDINATES: protein motif:HMM:NF018154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HRK25274.1"
                     /translation="MTVIAGPNGSGKTTLLRALVSQGLDAGVHINADDIALTLDASAE
                     RDRRAQGLAQEVRDDCIASGTTFTFETVMSHPSKVEEMGLAVRKGFKVRLIYVGLEDP
                     IINRERVGLRVKQGGHDVPADRIAARYERSMRLLPLAILTAHEALVFDNSDADRGHRL
                     VAVCTRTTIGGKRSLVVEMRGDARWFRAIVEAPEYGLRVQCRKKRLGFEESAE"
     gene            4474..5586
                     /locus_tag="PLQ11_10010"
     CDS             4474..5586
                     /locus_tag="PLQ11_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016650879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HRK25275.1"
                     /translation="MKSRAAVAWAANKPLTIETITIQPPKAGEVLVEVMATGVCHTDA
                     YTLSGLDSEGKFPAILGHEGAGIVREVGKGVTSLAVGDHVIPLYTPECRQCKTCLSQR
                     SNLCTAIRATQGQGVMPDGTSRFSCDDATVFHYMGCSTFSNFTVLPEIALAKVRKDAP
                     FDKICYIGCGVTTGIGAVINTAKVWPGANVAVFGLGGIGLNVIQGARMVGADRIIGID
                     INPKKADMARDFGMTHFVNPKAVGNDKVVQAIVDLTGGGADFSFDATGNTDVMRQALE
                     CCHRGWGESIIIGVAEAGKEIATRPFQLVTGRVWKGTAFGGARGRTDVPKIVDWYMEG
                     KINIDDLITHTMPLDQINAAFDLMHEGRSIRSVVKF"
BASE COUNT         1069 a         1805 c         1867 g         1012 t
ORIGIN      
        1 gtcagcggcg cgccggcacg cgccgaactg gcataggagg catggcgcct cagcgcgtat
       61 tcggcgatca gaatgccgcc gaccagcacc aggcagccca gcgccacctg cgccgcgccg
      121 ggaagattgc cgcgattgag ccagagggaa cggacagtca ggctcagcga ctggacgccg
      181 agatgctcga cagctccgac atcattgagc gtttcaagca gcgcaaacgc tgtgccggcg
      241 gccaccgccg gccgggccag cggcagcgcg atgcggaaaa acagccgcgc cccgacagcc
      301 ccgtgcagtc gcgccgcata gatgatgcgc cccacctgcc gctcgaacat cagccgggcc
      361 ggcagataga tgtagggata aagcaccagc gacaggatga tgatggcgcc gggcagggtg
      421 cgcacttcga agaaccagta gtcgctgcgg ctggaaacgc ccagcgccca gcgcagcatc
      481 cgttgcaccg gtccgaaata gtcggtgagt tccaccgcga cataggccgc aagataggtc
      541 ggcagcgcca gcggcagcac cagcatcacc gccagtacct ctcgccccgg gaaccggtag
      601 ctggcgatca gccaggcagc gccgacaccg atcgctgccg acaggatacc gactccggcc
      661 agcagcagca gcgtttcctg caaggtcgct gccagaccgg cattccatcc cagatgcgtg
      721 cccgcggctg gtccgaaggc aagcgctgcc agaccggcca agggcgctgt cagcagcaga
      781 ccgtagccga tcagcgccag tcgtaacggc cggctgccgc cgggcgcggc gcgctgtttc
      841 agggcagggc cggccgcagc ggtggtcatc cgccggggcc accgttgaag ccgatgcggt
      901 cgatcatgtc ggcggcgcgg ctgcgctggc ggatgatctc ggcaaacggc acttcatccg
      961 gggccaaagc accaaaattg gccactgccg gatggatcgc cgtgccgttc cgcaccggga
     1021 attcaaaatt gctttcggca tagatccgct gcgccttgtc gccggtcaga taggcggcaa
     1081 gcttctcggc gctggcggga ttgggaccgc tcctcgtcag ggcgatgccg gagaggttca
     1141 catgggtgcc gccgcccgaa aacttcggca ggatcaccct gatggcctcg gcccagggct
     1201 tctgatccgg attctgcagc atcaagccga cataataggt gttgcccagg ccgatgtcgc
     1261 aaagcccggc agcgatatcc ttggcgacct cgcgatcgcc gcccgagggc ttcttggcaa
     1321 gattggcctt caggcccttc agccatgtct cggccttgtc ggcgcccata tgggcgatgc
     1381 cggcggcgaa cagcatgttg ttgtagatgt gctggccatc gcgcaggcag aaacggccct
     1441 tccagcgcgg tgcggccaga tcctcatagg tgaggccggc gtccttgacg cgctccttcg
     1501 agacataggc aacacgcgcc cggtaggaca gaccgaacca gtgcccgtcg cgatcgcgca
     1561 gcgcctgcgg cacggccgct tccagctctg ccgatttcac cggacgggtg atccccagtt
     1621 caaccgcgcg caccagacgc gccacatcga cggtgatcag cgcatcggca gggctgcgcg
     1681 ccccttccga ggcgatgcgc tcctccagcc cttccttggc aaagaccaca ttgaccctta
     1741 tgccggtatc cgcagtaaaa gcatcaaaaa tgggcttgat cagccccggc tcgcggtagg
     1801 tgtagacatt gacctcctgc gcaaccgcag ggctggccat catcattccg gcaaggacca
     1861 ttgccctgcc tgtcatcgtc tgtctcattg acgtctccag caacgcgggt tcaaggcccg
     1921 gtgaccgatc ggaagcccct gcttccacgc ctgctgataa tcgcaaggcc tgtcttgtgc
     1981 aacaaaaatc cgtgcaattt cagtaagtta tagaagttct aaagtgacgg attctggaat
     2041 ggatcgaaac tgcccggcaa ggcacggttt cccttggcat tttttgcccg gtggcggata
     2101 gtgtcggcca ggatgagagg ggggcgggcg tgcgcaccag ggtcgccatc atcggctcgg
     2161 gaccatcggg cctgctgctc ggccggctgc tgagccaggc cgggatcgac aatatcctta
     2221 tcgacaccaa atcgcccgaa tacatcctcg gacgaatccg ggccggcgtt ctcgaacagg
     2281 gcgcggtcaa tctgctcgaa gaggccggtg ccggggcaag gatgcgccgc gaagggctgg
     2341 tgcatgacgg attcctgatc agctttgatg gccagacgca tcggatggat ctcagccgcc
     2401 attcgggcgg gctgtcggtg atggtctatg gccagaccga actgacgcgc gacctgatgg
     2461 aggcccgcaa ggctgatgct gcgccctcgc tctacgaagc cgaggcggtg gctattcacg
     2521 atttcgaccg gtcgcgaccg cgcgtcaccc tgcggcatgg cggcaagcct gtcacgatca
     2581 acgccgattt catcgccgga tgcgacggtt ttcacggcgt ctgccgcgcc agtgttccgg
     2641 ccggagccat caccgggttc gagcgcgtct atccgtttgg ctggctgggc ttgctcgccg
     2701 atgttccgcc ggtgcatgaa gagctgatct acgcccatca cgagcggggc tttgcgctgt
     2761 gttcgcagcg ttcgcgcagc cgcagccgct attacgtgca atgcagcctc gatgatacgg
     2821 tcgatgcctg gtcggatgca atgttctggg aggaactgcg tcgccgtctg ccggccgagg
     2881 ttgccgcgcg ggtcaagacc ggcccgtcga tcgagaagtc cattgcgccg ttgcgcagct
     2941 tcgtcgccga gccgctgcgg ttcggcacgc tgttcctggc gggcgatgcc gggcatatcg
     3001 tgccgccgac cggcgccaag gggctcaacc tggccgcctc cgacatccgc tacctgttcg
     3061 acgggctggt cgatttctat gcgcgcggct cgaccacagg gctggacagc tattcgcaac
     3121 gggcgctggc ccgcatctgg aaggccgaac gcttctcgtg gtggatgacc aatctgctgc
     3181 acagcttccc gcacgcttct ggattcgaac ggcgcatgca gcgcaccgaa ttcgactatc
     3241 tcatcagttc cgaggcggcc cagcgcgcgc tggccgagaa ttacgtcggc ctgccatact
     3301 gaggccggca gtggcgatca cagaatggcc cgaggaatac cggatttcgt tggggaaata
     3361 tcaagcttga ctgtgcattc tgttgccagg tttagacgtc ttgtgtgagc atgtgacatg
     3421 aagtccaata tcggcaaggc atcggcggat gccatcgcga agatcgctgc gggcgctggc
     3481 cttgcggcca gtgcaaaggc taaggcgcag ggacacgccg tcaccggaac gcgtatggtc
     3541 gagatcgacg gcaagcagaa gctgtgcgtg gtcaggattt atccatccgg cgatatggcg
     3601 gtggtgaaag ttctggaccc cgccacccca cagacggtcc gcacttcccg ccgctcatcc
     3661 aagagacgcg ctccagcaga ggctggccag accaagtaag gtgccatgcc gaatcgcccg
     3721 gtaatgacgg tcattgcagg gccgaatgga tcaggaaaaa cgaccttgct gcgggcgctg
     3781 gtctcccagg gtctggatgc cggggtccat atcaacgctg atgatatcgc tctgacgctt
     3841 gatgcttctg cggaacggga tcggcgcgca caaggactgg cacaagaggt tcgcgacgat
     3901 tgcatcgcga gtggcaccac attcaccttc gagacggtca tgtctcaccc ctcgaaggtt
     3961 gaggaaatgg ggctggcggt tcgcaaaggc ttcaaggttc gcctcatcta tgtcggactg
     4021 gaagatccga tcatcaatcg tgaaagggtc ggtttgcggg tcaagcaagg cggtcacgat
     4081 gttcctgcgg atcgcatcgc ggcgcgttac gaacgcagta tgcggctgct gccgctcgca
     4141 atcctgaccg cccacgaagc gctggtcttc gacaacagcg atgcagatcg ggggcatcga
     4201 ctcgtagccg tctgcaccag gacgacgatc ggcggcaaga ggtcccttgt cgtcgagatg
     4261 cgcggcgatg cgcgctggtt ccgggcgatt gtcgaggcgc cggaatatgg cctgcgcgtg
     4321 caatgccgga aaaagcgcct cggttttgag gagagcgccg aatagacgct ggcgtccgtt
     4381 gtcgccttgc cggcagcgat cactgcatgc tgcccttgcc catcgggcct caggcttgcc
     4441 atagtggcga gatcaaggga gttttccagc atgatcaagt cgcgcgccgc tgtcgcctgg
     4501 gccgccaaca agccgctcac tatcgagacg atcaccatcc agccgccgaa ggccggagag
     4561 gtgctggtcg aggtgatggc gaccggcgtc tgccataccg atgcctacac gctgtcgggc
     4621 ctcgattccg agggcaagtt cccggcgatc ctcggccatg agggggcagg aatcgtgcgc
     4681 gaggtcggca agggcgtgac ctcgctggcg gtcggcgatc atgtcatccc gctctacacg
     4741 ccggaatgcc ggcagtgcaa aacctgcctg tcgcagcgtt cgaacctgtg caccgccatc
     4801 cgcgcgacgc aggggcaggg ggtgatgccg gacggcacat cgcgcttttc ctgcgacgac
     4861 gccaccgtgt tccactacat gggctgttcg accttctcga acttcaccgt gctgccggaa
     4921 atcgcgctgg ccaaggtgcg caaggacgcg ccgttcgaca agatctgcta catcggctgc
     4981 ggagtgacga ccggcatcgg ggcggtgatc aacaccgcca aggtctggcc gggggccaat
     5041 gtcgcggtgt tcggcctcgg cggcatcggg ctgaatgtca tccagggcgc gcgcatggtg
     5101 ggtgccgaca ggatcatcgg catcgacatc aacccgaaga aagcggatat ggcgcgcgat
     5161 ttcggcatga cgcatttcgt caatccgaag gcggtcggca acgacaaggt ggtgcaggcc
     5221 atcgtcgacc tgaccggcgg cggcgccgat ttttccttcg atgcgaccgg caacaccgat
     5281 gtgatgcgtc aggcgctcga atgctgccat cgcggctggg gcgagagcat catcatcggt
     5341 gtggcggaag ccggcaagga aatcgccaca aggccgttcc agctggtcac cgggcgcgtc
     5401 tggaagggaa cggccttcgg gggcgcgagg ggccgcaccg atgtgccgaa gatcgtcgac
     5461 tggtatatgg agggcaagat caacatcgac gatctgatca cccacaccat gccgctcgac
     5521 cagatcaacg cggccttcga cctgatgcac gagggacgca gcatccgctc ggtggtcaag
     5581 ttctgacgct tgttgtcagg tgtccggccc atgcctgtcg cgatcaaccg cggcccagtt
     5641 gatcgcggca tgctctattg ccgcgggatc agtggtgcac cgtcggccgg aacctggccg
     5701 ggcggcgtgc ggagcggccc gccggccagc attgccgcca atctctcgcc gga
//
LOCUS       DAOMXG010000154         5682 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_7516_length_5682_cov_9.647414, whole genome shotgun sequence.
ACCESSION   DAOMXG010000154 DAOMXG010000000
VERSION     DAOMXG010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 5682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5682
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7516_length_5682_cov_9.647414"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="PLQ11_10015"
     CDS             <1..483
                     /locus_tag="PLQ11_10015"
                     /inference="COORDINATES: protein motif:HMM:NF022738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3095 family protein"
                     /protein_id="HRK25276.1"
                     /translation="SAGCDRPIGRPEDLAVAPAGEWLDLETSTSRVGGFPRWTIRLER
                     ALFARLIGFVIAGDRKIGLFDAGRFKAEVVANAVSASLADGFRATIDCTPAHADAIGA
                     RLADAETAGIARFGLSRQDAAMMISFIPHWTSPNHLHFIDGAGGGLGAAERLMAEKTS
                     "
     gene            695..1654
                     /locus_tag="PLQ11_10020"
     CDS             695..1654
                     /locus_tag="PLQ11_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRK25277.1"
                     /translation="MISKRVVLGAAACAMLAGVAVAQAPAFFRIGTGGTAGTYYPVGG
                     MIANAVSEPPGLVATAVASNGSVANVNSIVSGAAEGGFSQADVAYWAFTGTGVFEGKP
                     KVEELRSIANLYPESVHVVVRKGVAKSIADLKGKKVSLDEPGSGTLINAKAILAAYGI
                     TEKDISAEFLKPNQAAEKLKDGAVDAFFFTGGYPAAAISELASTSAGIELLPIDGDGA
                     AKLKASSPFFADDAIPDATYKGIGAIKTVAVGAHLVTTAKQPEDVIYRITKAMWSDKT
                     RAVLDAGHAKGKVIRKETALSGIGGVPLHPGAAKFYKEAGLMK"
     gene            1776..3941
                     /locus_tag="PLQ11_10025"
     CDS             1776..3941
                     /locus_tag="PLQ11_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018690430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HRK25278.1"
                     /translation="MTQTAIPSADELQRLEEELDPEMSFRKMPEGTALIVGALLFALS
                     VFHYYTAGFGLLREATHRGIHLAFVVGLIFLVFAARVGDGKQVRPANALHPGGIPLID
                     WLLAAGAAASALYVPWIFTDLQFRVGNPNTLDLTMGSIAVVVLIEATRRCMGWGLPLI
                     ALFAMAYAYFGPWFPGIFLHPGASWSNIVNHLYLTSQGIYGVALGVVATYVFHFVLFG
                     VLATRVGLGRLFLGVAAAVAGRFAGGPAKISIFGSALFGMISGSSVANTVTVGSLTIP
                     MMIRLGYQRHFAAAVESTAATGGQITPPIMGAAAFLMVEFLNMPYATIVIAAIVPAIM
                     HFFGVLMQVHFEAKAHGMRGLREDEIPHLGTVLKEEWPTVAPLFALLVVLFSHYTPYL
                     AAFWGITGCMLLGFARNRAMAALVFMVAIAALAPTHVLRMPYANLILFGVALAAMIDG
                     YLASPQGRARLNDMVDGFIVGAKYAIAVGAAAATVGIIVGVVTLTGVGFKISWIVTTS
                     ADSMAKALMGALPFLPFEVKGVTLFFTLVMTAIVCILLGCGVPTTATYIIMVTVAAPA
                     LGMLGVAPIVAHFFVFYYGVLADITPPVAIAAYAGASMAGADPFKTGNTAFRLALGKA
                     LVPFVFAFSPALLIVAPGFTLMDFIVTTAGCMIGIVMLSAAFSAWWLAPLRMWERWWL
                     AAASLPTIAPGLVSTLIGIGLALPVLIRQIRMRQTGQAA"
     gene            3938..5098
                     /locus_tag="PLQ11_10030"
     CDS             3938..5098
                     /locus_tag="PLQ11_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRK25279.1"
                     /translation="MNQTGNTIRIATFNVENLFSRLSFADDAPNDDLLVGAYEFDDAE
                     ERKLARRIVEAAASDDKRQLTALALHAARADVACLQEVDNKAALDVFYARYLKRLIEP
                     AVAAEKRALRAEMLRSGQGVDLDKMFAVDKRHYFDWRVVVDGNDGRGIDVAVMSKLPV
                     SIRTHAHRTFQELGVWSDELRGYRDKVGGRERRLSPSDRVFMRDCLEIDLRVNGRPLT
                     LYTCHFKSMHPTRDHTRPYRRAEALAVRRIIEERFGRKTGEANWIICGDLNDYVEIDG
                     LPVVDDNGKLVPSALEPLIADGFSENVVRRLRPEERWTTYHGAEDQYAQLDFMLLSPA
                     LAAANPDMKPEIVRMGQPYRAVRYQGVRFPRTGWDRPKASDHSPIVCEIMVP"
     gene            5100..>5682
                     /locus_tag="PLQ11_10035"
     CDS             5100..>5682
                     /locus_tag="PLQ11_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRK25280.1"
                     /translation="MTAPAVQLSGVSISYALASGGRYDAVAPVDVTIADGAFVSIVGP
                     TGCGKSTLLNVAAGLAAPTTGQAEIFGEPLAGINAKAGYLFQQDALMPWKSALDNVAI
                     GLEVKGVARGEALREAQAMLVRVGLKAFGERYPHQLSGGQKKRVALAQVLVRHPKILL
                     MDEPFGPLDAQTRLIMGELLSGLWQADRKAVMFV"
BASE COUNT          894 a         1706 c         1966 g         1116 t
ORIGIN      
        1 tctgccggct gcgaccggcc gatcggccgg ccggaggatc tggcggtcgc gccggcgggc
       61 gagtggcttg atctggagac cagcaccagc cgcgtgggcg gatttccccg ctggacgatc
      121 cggctggagc gggcgctgtt tgccaggctc atcggctttg tcattgcggg cgaccgcaag
      181 atcggcctgt tcgacgcggg ccggttcaag gccgaggtgg tcgccaatgc cgtttcggcc
      241 agtcttgccg atggctttcg cgcgacgatc gattgcaccc cggcgcatgc cgatgcgatt
      301 ggcgcgcggc tggccgacgc tgaaaccgca ggcatcgccc gctttggcct gagccgtcag
      361 gatgcggcca tgatgatcag cttcatcccg cactggacat cgcccaacca tctgcatttc
      421 atcgacgggg cagggggcgg gctgggtgcg gccgagcggc tgatggcgga aaagacgtcc
      481 tgaaatcaat gcgacgggcg ttctggcaag atcacaccgt gtcttgattg acccatcaag
      541 acacggataa cgtgatcgat tacaagagtt tagagcatgc tagacgcgaa aagccgctca
      601 caccctttcg cagcatgctc taggcttgcc tcgaatgcca tcgctgtgcc actgtccggg
      661 cttggccaaa aggcttagat cagggagagg gcagatgatt tccaaacggg tcgttctggg
      721 cgcggcagca tgcgcgatgc tggcgggggt ggcggtggcg caggcgccgg cgttcttccg
      781 catcggcacc ggcgggacag ccggcaccta ttatccggtc ggcggcatga tcgccaatgc
      841 ggtttccgaa ccgcccggtc tggtggcgac ggccgtggcg tcgaacgggt cggttgccaa
      901 cgtcaattcc atcgtttccg gtgcggccga gggcgggttc agccaggctg acgttgccta
      961 ttgggcgttc accggcaccg gcgttttcga gggcaagccg aaggtcgagg aattgcggtc
     1021 gattgccaac ctctatccgg aatcggtgca tgtggtggtg cgcaagggcg tcgccaagtc
     1081 gattgccgac ctcaagggca agaaggtctc gctcgatgag ccgggctcgg gcacgctgat
     1141 caatgccaag gcgatccttg ctgcctatgg catcacggag aaggacattt cggcagaatt
     1201 cctcaagccc aaccaggcgg cggaaaagct gaaggatggc gctgtcgatg cgttcttctt
     1261 caccggcggc tatccggcgg cggcgatttc ggaactggcc tcgaccagcg ccggcatcga
     1321 gctgctgccg atcgacggcg acggcgcggc caagctcaag gcctcctcgc cgttctttgc
     1381 cgatgatgcg attccggatg cgacctacaa ggggatcggc gcgatcaaga ccgttgcggt
     1441 cggcgcccat ctcgtgacca cggccaagca gcccgaggat gtgatctacc gcatcaccaa
     1501 ggcgatgtgg agcgacaaga cccgcgccgt tctcgatgcc ggccatgcca agggcaaggt
     1561 catccgcaag gagacggcgc tttcgggcat cggcggggtt ccgctgcatc cgggcgctgc
     1621 gaagttctac aaggaagccg gcctgatgaa gtgacgggag gctgacccaa gcctcctcgt
     1681 catggtcgga tccggtccgg ccgtaaggtt gcacagtcgg cgatggtcgg gcgaggtccg
     1741 gccatcgcga cgttgatgat tggccagatg atcccgtgac ccagaccgcg ataccctccg
     1801 ctgacgaatt gcagcggctc gaggaagaac tcgatcctga aatgtccttc cgcaagatgc
     1861 cggaaggaac ggcgctgatt gtcggcgcgc tgctgttcgc gctgtcggtg ttccactatt
     1921 acactgccgg cttcggcctg ttgcgcgagg caacgcacag gggcatccat cttgcctttg
     1981 tcgtcgggct gatcttcctg gtgtttgcgg cgcgggtcgg cgacggcaag caggtgcgtc
     2041 cggccaatgc gctgcatccc ggcggcattc cgctgatcga ctggctgctg gctgcgggag
     2101 cggcggcctc ggcgctctac gttccatgga ttttcaccga tctgcaattc agggtcggca
     2161 atccgaatac actcgatctg accatggggt cgatcgctgt cgtggtgctg atcgaggcga
     2221 cgcggcgctg catgggctgg ggcctgccgc tgatcgcgct gtttgccatg gcttacgcct
     2281 atttcggccc gtggtttccc ggtattttcc tgcatccggg cgcaagctgg tcgaacatcg
     2341 tcaaccatct ctatctcacc agccagggca tctatggcgt cgcgctcggt gtggtggcga
     2401 cctatgtgtt ccacttcgtg ctgttcggtg tgctggcgac gcgggtcggc ctcgggcggc
     2461 tgttcctcgg cgtggcggcg gcggtggccg ggcggttcgc cggggggcct gcgaagattt
     2521 ccatcttcgg ttcggcgctg ttcggcatga tttccggctc gtcggtggcc aatacggtga
     2581 cggtcggctc gctgaccatc ccgatgatga tccggctcgg ctaccagcgg cattttgcgg
     2641 cggcggtgga atcgacggcg gcgaccggcg ggcagatcac cccgccgatc atgggcgcgg
     2701 cggcgttcct gatggtcgaa ttcctcaaca tgccctatgc gaccattgtc atcgcggcga
     2761 tcgtgccggc gatcatgcac ttcttcggcg tgctgatgca ggtgcatttc gaggccaagg
     2821 cgcatggtat gcgcgggctg agggaggacg agatcccgca tctgggcacg gtgctgaagg
     2881 aggaatggcc gacggttgcg ccgctgtttg cgctgctggt ggtgctgttc tcccattaca
     2941 cgccgtatct cgctgccttc tggggcatta ccggctgcat gctgctcggc tttgcccgca
     3001 acagggcgat ggcggcgctg gtgttcatgg tggcgattgc tgcgctggcg ccgacgcatg
     3061 tgctgcgcat gccctatgcc aacctgatcc tgttcggtgt ggcgctggcg gcgatgatcg
     3121 acggctatct ggcctcgccc cagggcaggg cgcggctcaa tgacatggtg gacgggttca
     3181 tcgtcggggc caaatacgcc attgccgtag gcgcggcggc ggcgacggtg ggcatcatcg
     3241 tcggcgtggt gacgctgacc ggcgtcggct tcaagatttc gtggatcgtg acgacttcgg
     3301 ccgattcgat ggcgaaggcg ctgatggggg cgctgccgtt cctgccgttc gaggtcaagg
     3361 gcgtgacgct gttcttcacg ctggtgatga cggcgatcgt ctgcatcctg ctcggctgcg
     3421 gcgtgccgac gacggcgacc tacatcatca tggtgacggt ggcggcgccg gcgctcggca
     3481 tgctgggggt tgcgccgatc gtcgcgcatt tcttcgtgtt ctattacggg gtgctggccg
     3541 acatcacgcc gccggtggcg atcgccgcct atgcgggggc ctcgatggcc ggggccgatc
     3601 cgttcaagac cggcaatacg gcattccggc tggcccttgg caaggcgctg gtgccgttcg
     3661 tctttgcctt ctcgccggcg ctgctgatcg tcgcgccggg gttcacgctg atggatttca
     3721 tcgtcaccac cgccggctgc atgatcggta tcgtgatgct gtcggcggcg ttttcggcct
     3781 ggtggctggc accgctcaga atgtgggaac gctggtggct ggcggctgcc tcgctgccga
     3841 ccatcgcgcc ggggctggtg tcgaccttga tcggcatcgg ccttgccctg ccggtgctga
     3901 tccgccagat ccggatgcga cagacgggac aggcagcatg aaccagacgg gcaacacgat
     3961 ccgcatcgcc acgttcaatg tcgagaacct gttctcgcgg ctgtcgttcg ccgatgatgc
     4021 accgaatgat gacctgctgg tcggcgccta tgagttcgac gatgccgagg aacgcaagct
     4081 ggcgcgtcgc atcgtcgagg cggcggccag cgatgacaag cggcagttga cggcgctggc
     4141 gctgcatgcc gcccgcgccg atgtcgcctg cctgcaggag gtcgataaca aggcggcgct
     4201 ggatgtgttc tacgcacgct atctcaagcg cctgatcgag cccgcagtcg cagcggaaaa
     4261 gcgggcgctg agggccgaga tgctgcgcag cgggcagggg gtggacctcg acaagatgtt
     4321 tgctgtcgac aagcgccatt atttcgactg gcgcgttgtt gttgatggca atgatggccg
     4381 cggcatcgat gtggcggtga tgtccaagct gccggtgagc atcaggaccc acgcgcatcg
     4441 cacctttcag gaactcggcg tctggtccga cgagctgcgt ggctatcgcg acaaggtggg
     4501 cgggcgtgag cggcggctgt cgccctccga ccgggtattc atgcgcgatt gcctggaaat
     4561 cgacctcagg gtgaatggcc ggccgctgac gctttacacc tgccatttca agtcgatgca
     4621 tccgacccgc gaccacacgc ggccctatcg ccgcgccgag gcattggcgg tgcggcgcat
     4681 catcgaggag cggttcggcc gcaagaccgg tgaggccaac tggatcatct gcggcgatct
     4741 caacgattat gtcgagatcg acgggctgcc ggtggtcgat gacaacggca agctggttcc
     4801 gagcgcgctg gagccgctga tcgccgatgg gttttcggag aatgtcgttc ggcgcctcag
     4861 acctgaagag cgctggacca cctatcacgg cgccgaggat caatacgccc agcttgactt
     4921 catgctgctg tcgccggcgc tggccgccgc caatcccgac atgaaaccgg agatcgtgcg
     4981 gatggggcag ccctaccggg cggtgcgcta tcagggcgtg cgcttcccgc gcaccggatg
     5041 ggacaggccg aaggcctcag accattcgcc gattgtctgc gagatcatgg tgccgtgaca
     5101 tgacggctcc cgcagtccag ctttccggtg tctcgatctc ctatgccctg gccagtggcg
     5161 ggcgctacga tgccgtggcg ccggtggatg tcaccatcgc ggacggcgcc ttcgtttcca
     5221 tcgtcgggcc gaccggatgc ggaaaatcga ccctgctcaa tgttgcagca gggctggcgg
     5281 cgccgaccac cggacaggct gagattttcg gcgagccttt ggccgggatc aacgccaagg
     5341 ccggctacct gttccagcag gatgcgctga tgccgtggaa gagcgcgctc gacaatgtcg
     5401 ccatcggcct ggaagtgaag ggcgttgccc ggggcgaggc gttgcgcgag gcgcaggcga
     5461 tgctggtccg cgtcggcctc aaagccttcg gagagcgcta tccgcaccag ctttccggcg
     5521 ggcagaaaaa gcgtgtggcg ctggcgcagg tgctggtgcg gcatccgaag atcctgctga
     5581 tggacgagcc gttcggcccg cttgatgcgc agacgcggct gatcatgggc gagttgctgt
     5641 cgggcctgtg gcaggcggac cgcaaggcgg tgatgttcgt ca
//
LOCUS       DAOMXG010000155         5626 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_7631_length_5626_cov_11.114522, whole genome shotgun sequence.
ACCESSION   DAOMXG010000155 DAOMXG010000000
VERSION     DAOMXG010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 5626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5626
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7631_length_5626_cov_11.114522"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..1446)
                     /gene="leuC"
                     /locus_tag="PLQ11_10040"
     CDS             complement(40..1446)
                     /gene="leuC"
                     /locus_tag="PLQ11_10040"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019170838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HRK25281.1"
                     /translation="MATPRTLYDKIFDDHVVDRYPDGTCLLYIDRHLVHEVTSPQAFE
                     GLRMSGRKVRHPEKTLAVVDHNVPTTDRSHGIEEPESRLQVETLAENAKNFGVEYYNE
                     HDRRQGIVHAVGPEQGFTLPGTTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTL
                     IQAKAKNMLVRVDGRLAPGATAKDIILAIIGEIGTGGGTGHVIEFAGDAIMALSMEGR
                     MTVCNMTIEGGARAGLIAPDEKTFAYLKGRPKAPTGAKWDMAMEYWKTLKTDEGAHFD
                     RIVTLDAANLPPIVSWGTSPEDVVSVQGVVPDPAAIADESKRLSKQRALDYMGLKAGT
                     KITDIAIDKVFIGSCTNGRIEDLRAAAGVLRGRRIAASVSGIVVPASGLVKEQAEAEG
                     LHQVFIDAGLEWREPGCSMCLGMNPDQLKPGERCASTSNRNFEGRQGYRGRTHLVSPE
                     MAAAAAVAGRFVDIRSFS"
     gene            1656..3635
                     /locus_tag="PLQ11_10045"
     CDS             1656..3635
                     /locus_tag="PLQ11_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HRK25282.1"
                     /translation="MANTSEPQDSAASPAAPSAAPVVRPEPGHSAAADHGHGKEGPLA
                     LALGSIGVVYGDIGTSPLYAMRESLHHYAHGGPIDPAAVYGVISLFIWALTLVVTVKY
                     VIFIMRADNRGEGGTLTLMALAQGVLGRRAFLPAALGIVGAALFYGDSIITPAISVLS
                     AVEGLKYITPVFERYILWISIAILVGLFAVQSRGTGRVAAFFGPIMLVWFLVLGGLGL
                     MHIGDDLSIFAAFNPVYGIQFLLGHGILGLLVLGSVFLSVTGAEALYADMGHFGRLPI
                     RLAWLVVIFPCLTLCYLGQGAMILAHPETAKDPFYMMVPESWGLMGKAPLVILATLAT
                     VIASQAVITGAYSLTQQAIRLGLLPRLEIRHTSETQLGQIYLPQINNLLLIGVLALVV
                     MFQTSSKLANAYGIAVTGAMLADTALFFLVAWKVWKWKVSLVTCLVLPLFLIDLAFFG
                     ANALKIPDGAYMPLIFAALLIVLIWTWLRGTRIVANKEKKDSLPLVDLIKMLERSKPV
                     RVPGTAVFLCSDADTAPAALLHNLKHNKVLHKRNIMMNIRTAEVPRVPEHEQLRIEDL
                     TEDVKLVIATLGYMETPRVPHLLALARRRGLNFDILQTSFFLGRRSIKPAQHSGMPLW
                     QDKLFISLAKLATNATDFFQIPSGRVVEMGSQVTI"
     gene            complement(3711..4595)
                     /locus_tag="PLQ11_10050"
     CDS             complement(3711..4595)
                     /locus_tag="PLQ11_10050"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRK25283.1"
                     /translation="MQQLNQEVIDLYDYFIHSDMDRRVFMDRLVRLVGGAAAANAALA
                     LLQSNYALAQTVPENDPRLVSETWSYTGPHGTMNGSLVRLRGGARRPAVLVIHENRGL
                     NPHIRDVTRRIALEGFLAFGIDSLSLDGGTPADEDAARAMFAKVDLNRIGESVAAAVK
                     ALSAHPEATGKVGAVGFCWGGGMVNQLAWRAPDLAAGVAYYGMQAPADKVPAINAALL
                     LHYAGTDERINAGIETYRKALTDAGKTFEIHLYEGTQHAFNNDTGGARYNAEAARLAW
                     SRTIAHFVKHLGAPPKPS"
     gene            complement(4748..5122)
                     /gene="rplS"
                     /locus_tag="PLQ11_10055"
     CDS             complement(4748..5122)
                     /gene="rplS"
                     /locus_tag="PLQ11_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018235930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HRK25284.1"
                     /translation="MNVIAEIEKEEANRLADGKSIPKFQAGDTLQVLVKVVEGDRTRV
                     QAYEGVCIARSGGGLNESFTVRKISYGEGVERVFPMYSPLIEGIKVVRRGHVRRAKLY
                     YLRDRRGKSARIAERAEKVSDA"
     gene            complement(5195..>5626)
                     /locus_tag="PLQ11_10060"
     CDS             complement(5195..>5626)
                     /locus_tag="PLQ11_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRK25285.1"
                     /translation="QAAARELAAGPGALIVCGRFEGVDERVIGARSLQEFSIGDYILS
                     GGEIAAMVLLDAVVRLIPGVMGKQASGTEESFEAGLLEYPHYTRPRNFEGQPIPDVLT
                     SGDHARIARWRREAAERLTAERRPDLFEKHLAARQRGKTDV"
BASE COUNT          935 a         1760 c         1815 g         1116 t
ORIGIN      
        1 ccggatgact ccggcgcctt ggttttggag ccttccagtt caggaaaagc tgcggatatc
       61 gacaaaacgc ccggccaccg ccgccgccgc cgccatttcc ggcgagacaa gatgggtgcg
      121 gccgcgatag ccctgccgcc cttcgaagtt gcggttcgac gtcgaggcgc agcgttcgcc
      181 cggcttgagc tggtccgggt tcatgccaag gcacatcgag cagcccggct cgcgccattc
      241 gagaccagca tcgatgaaca cctgatgcag accctcggcc tcggcctgct ccttgaccag
      301 gccagaggcc ggcacaacaa tacccgaaac gctcgcggcg atcctgcgtc ccctgagaac
      361 gccggcagcg gcacgcagat cctcgatgcg gccattggtg caggagccga tgaacacctt
      421 gtcgatggcg atgtcggtga tcttggttcc cgccttgagg cccatgtagt ccagggcgcg
      481 ctgcttggac aggcgcttgg attcatcggc gatcgccgcc ggatccggca ccacgccctg
      541 aaccgagacc acatcctccg ggctggtgcc ccaggagacg atcggcggca ggttggcggc
      601 gtcgagcgtg acgatgcggt cgaaatgcgc cccttcgtcc gtcttcaacg ttttccaata
      661 ctccatggcc atgtcccatt tggcgccggt cggcgccttg gggcggccct tgagataggc
      721 gaatgtcttt tcgtccggag cgatcaggcc ggcgcgggcg ccgccctcga tggtcatgtt
      781 gcagaccgtc atgcggcctt ccatcgacag ggccatgatg gcatcgccgg caaattcgat
      841 gacatggccg gtgccaccgc cagtgccaat ctcgccgatg atggcaagga tgatgtcctt
      901 ggcggtcgcg cccggggcaa gcctgccatc cacgcggacc agcatgttct tggccttggc
      961 ctggatcagt gtctgggtcg ccagcacatg ctcgacctct gaggtgccga tgccatgggc
     1021 gagcgcaccg aaggcgccgt gggtcgaggt gtggctgtcg ccgcagacga tcgtggtgcc
     1081 gggcagggtg aagccctgtt cggggccaac cgcatggacg atgccctgcc gccggtcatg
     1141 ttcgttgtaa tattcgacgc cgaaattctt ggcgttttcg gccagagtct cgacctggag
     1201 ccggctttcc ggctcctcga tgccgtggct gcgatctgtg gtcggcacgt tatggtcaac
     1261 gaccgccagc gtcttttcgg ggtggcggac cttgcggcct gacattctca acccctcgaa
     1321 ggcctgcggc gaagtgacct catggacgag gtgacggtca atatacagca gacaggtccc
     1381 gtcaggataa cgatcaacca catgatcgtc gaaaatcttg tcgtagagcg tgcgcggggt
     1441 cgccatcgtc accaaaacct tcaaaggggg tggataggga agcggtcctt atacgaaatt
     1501 gcaggtgcga gaaaagagcg aatccatatc cgggccgcta tcaggaggtt cttcaagcca
     1561 acaacatgct tttcggtttc ccccaagccg tctataagcc cccttctgtt ggcctgatgc
     1621 cttcggccaa actgagccga atccggattc gccccatggc aaatacctct gaaccccaag
     1681 actctgccgc ttcccctgct gcgccctctg ctgctcctgt cgttcggccg gagcccgggc
     1741 actccgcggc ggccgaccac gggcatggca aggagggccc gctggctctt gcgctcggct
     1801 cgatcggcgt cgtctacggg gatatcggaa cctctccgct ctatgcgatg cgcgagtcgc
     1861 tgcaccatta tgcccatggc ggcccgatcg atcctgccgc cgtctacggg gtgatctccc
     1921 tgttcatctg ggcgttgacg ctggttgtga cggtaaaata cgtcattttc atcatgcgcg
     1981 ccgacaacag gggcgagggc ggaacgctca ccctgatggc gctggcgcag ggtgtgctcg
     2041 ggcgccgagc ctttctgccg gccgcgctcg gcattgtcgg ggcggcgctg ttctatgggg
     2101 attcgatcat cacgccggcg atttcggttc tgtcggcggt cgagggcctg aaatacatca
     2161 cgcccgtttt cgaacggtac atcctgtgga tctcgattgc cattctggtc gggttgttcg
     2221 cagtccaaag ccgcggcact ggcagggttg cggctttctt cgggccgatc atgctggtct
     2281 ggtttctggt tctgggcggc ctcgggctga tgcatatcgg cgatgacctg tcgatttttg
     2341 ccgccttcaa tccggtttac ggcatccagt tcctgctcgg ccacgggatc ctggggttgc
     2401 tggtgctggg ctcggtgttc ctctcggtga cgggggcgga agcgctctat gccgatatgg
     2461 gccacttcgg ccggctgccg atccggcttg cctggctggt ggtaatcttt ccctgtctga
     2521 cgttgtgcta tctcggtcag ggggcgatga tccttgccca tccggaaacg gccaaggatc
     2581 cgttctacat gatggttccg gaaagctggg gcctgatggg caaggcgccg ctggtgatcc
     2641 tggcgacgct ggcaaccgtc atcgccagtc aggcggtgat caccggggcc tattcgctga
     2701 cccagcaggc gatccggctt ggcctgctgc cgcggctcga gatccgccat acttcggaaa
     2761 cccagctcgg ccagatctat ctgccgcaga tcaacaacct gctgctgatc ggcgtgctgg
     2821 cattggtggt gatgttccag acctcgtcaa agctggccaa tgcctatggc atcgcggtga
     2881 ccggcgccat gctggccgat acggcgctgt tcttcctggt ggcgtggaag gtgtggaagt
     2941 ggaaggtgtc gctggtgacc tgcctggtgc tgccgctgtt cctgatcgac ctggcgttct
     3001 tcggggccaa tgcgctgaag attccggatg gtgcctatat gccgctgatc ttcgcggccc
     3061 tgctgatcgt gctgatctgg acctggctgc gtggaacgcg catcgtcgcc aacaaggaaa
     3121 agaaggacag cctgccgctg gtggatctca tcaagatgct ggaacggtcc aagcctgtgc
     3181 gggtgcctgg aacggcggtg ttcctgtgtt cggatgccga tacggcgcct gctgcattgc
     3241 tgcacaacct caaacacaac aaggtgctgc acaagcgcaa catcatgatg aacatccgca
     3301 ctgcggaagt gccgcgcgtt ccggagcacg aacagttgcg gatcgaggat ctgaccgagg
     3361 acgtcaagct ggtcatcgca acgctcggtt acatggaaac cccgcgcgtg ccccatcttc
     3421 tggcgttggc gcggcgacgt gggctcaatt tcgatattct gcagacgtcg ttcttcctcg
     3481 ggcgacggtc gatcaagccg gcgcagcatt ccggcatgcc gctctggcag gacaaattgt
     3541 tcatttccct ggccaaactc gccaccaatg cgacggactt cttccaaatt ccgtccggcc
     3601 gtgtggtgga aatgggcagt caggtgacga tctgaggccg gaaccgggga tttgcgctcc
     3661 ggttccgcac aacccttcag cgttcgccgc tgcgacggcg ccttaacctg tcaggaaggt
     3721 tttggcggcg cgccgagatg cttgacgaaa tgcgcgatgg tgcggctcca ggcgagcctg
     3781 gcggcctcgg cgttgtagcg ggcgccgccg gtatcgttgt tgaaggcgtg ctgggtgccc
     3841 tcatacaggt ggatctcgaa ggttttgccg gcatcggtca gggccttgcg ataggtctcg
     3901 atgccggcgt tgatgcgctc gtccgtgccg gcatagtgca gcagcagggc cgcgttgatg
     3961 gcgggcacct tgtcggccgg agcctgcatt ccgtaatagg ccacgcccgc cgcaaggtcc
     4021 ggcgcgcgcc aggcaagctg gttgaccatg ccgccgcccc agcagaagcc gaccgcgccg
     4081 accttgccgg tcgcttcagg atgggccgag agcgccttga ccgccgccgc gacgctttcg
     4141 ccgatccggt tcagatcaac cttggcgaac atggcgcgcg cggcatcctc gtcagcgggt
     4201 gtgccgccgt cgagcgacag ggaatcgatc ccgaatgcca ggaagccctc gagcgcgatc
     4261 cggcgcgtca catcgcggat atgcgggttg agaccgcgat tctcatggat caccagcacg
     4321 gcggggcgtc gtgcgccacc cctgaggcgg acgagggaac cgttcatcgt cccgtgcgga
     4381 ccggtatagc tccaggtctc gctgaccagc cgcgggtcat tctccggcac cgtctgggcc
     4441 agcgcgtagt tcgattgcag cagcgccagc gcggcattgg cggcggcggc cccgccgacc
     4501 agcctcacca gccggtccat gaatacgcgg cggtccatgt cggaatggat gaaatagtcg
     4561 tagaggtcga tgacctcctg attgagctgc tgcatgatgc ctctccctgc cggcatgcgc
     4621 aatttgtctc ccgatcgaaa caaacggctc cagcgccaag cctaatctag gcgcgcaggg
     4681 cggcaaatga aaagcgcggg gggggggtgc cccgcgctcg tcatcacaaa ccggtgacga
     4741 gcctggttca ggcgtcgctg accttctcgg cgcgctcggc gatgcgggcg gacttgccgc
     4801 gacggtcgcg cagataatac agcttggcgc ggcggacatg gccgcggcgg accaccttga
     4861 tgccctcgat cagcggcgaa tacatcggga agacgcgctc gacgccctcg ccgtaggaaa
     4921 tcttgcggac ggtgaagctc tcgttcagcc cgccgccgga gcgggcgata cagacgccct
     4981 cataggcctg cacgcgggtg cggtcacctt caaccacctt gacgagcacc tgcagggtgt
     5041 cgccggcctg aaacttgggg atcgacttgc catcggcaag acggttggct tcttcctttt
     5101 cgatctcggc gatcacgttc atcggactgc tccattccgt tgtgccggcg ggccggcaag
     5161 cgcttttggg acgctgtttt gagttggtgg cggctcatac atcggttttg ccgcgctgtc
     5221 gagcggcgag gtgcttttcg aaaaggtccg gccggcgttc ggctgtcagg cgctcggccg
     5281 cctcccgccg ccagcgggca atgcgggcgt gatcgcccga ggtgagcaca tccgggatcg
     5341 gctggccctc gaaattgcgc gggcgggtgt agtgcgggta ttccagcagg ccagcctcga
     5401 aactctcctc ggtgccggat gcctgcttgc ccatcacgcc ggggatgagg cgcaccaccg
     5461 catcaagcag aaccatggca gcgatctcgc cgccggacag gatgtaatcg ccgatcgaga
     5521 attcctgaag gctgcgggcg ccgatcaccc gctcatcgac gccttcgaaa cggccacaga
     5581 caatcaaagc gccggggcca gcggccagct cgcgcgccgc cgcctg
//
LOCUS       DAOMXG010000156         5535 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_7782_length_5535_cov_9.598905, whole genome shotgun sequence.
ACCESSION   DAOMXG010000156 DAOMXG010000000
VERSION     DAOMXG010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 5535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5535
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7782_length_5535_cov_9.598905"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="PLQ11_10065"
     CDS             complement(<1..836)
                     /locus_tag="PLQ11_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRK25286.1"
                     /translation="MPISDRFPFAMLVCVCCLAGPAEALDANAQPEGKAPPIESFKSV
                     TDALKAGVRGLNAGDKPGAAKALNYAATKGDIAAQFKLGRMYAEGDGVEQDDYRAFRL
                     YQQIANANAEEVRGTMKAGVVAKAFVQLGSYYLDGIPGSPVKANPDRAYEMFHYAASY
                     FGDPDAQYNVGRMYLDGAHGEKDARQAARWFNLAAEKGHPYAQAVLGQILFNGQNGVP
                     RQAPLGLSWMLLARDACDPHKDAWIFDMTRKAETAASDDERRMAKTYARKFARATPAG
                     EAP"
     gene            complement(823..2547)
                     /gene="ilvD"
                     /locus_tag="PLQ11_10070"
     CDS             complement(823..2547)
                     /gene="ilvD"
                     /locus_tag="PLQ11_10070"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRK25287.1"
                     /translation="MDKRVTDKSRLPSRHVTEGPARAPHRSYYYAMGLTKDQIHQPFV
                     GVASCWNEAAPCNIALMRQAQAVKKGVASAGGTPREFCTITVTDGIAMGHQGMKSSLA
                     SREVIADSVELTMRGHCYDALVGLAGCDKSLPGMMMAMCRLNVPSIFIYGGSILPGTF
                     RGRPVTIQDMFEAVGKHSVGEMSDADLEEIEQVACPSAGACGAQFTANTMATVSEAIG
                     LALPYSAGAPAPYEIRDRFCMTAGEMIMELIAMNIRPRDIVTREALENAATVVAASGG
                     STNAALHLPAIAHECGIAFDLFDVAEIFKRTPYIADLKPGGRYVAKDMFEVGGIPLLM
                     KTLLDHGYLNGDCLTVTGRTIAENLESVRWNDDQDVVRPANKPITPTGGVVGLSGNLA
                     PDGAIVKVAGMKVLRFTGPARCFDSEEACFEAVSKRAYKEGEVLVIRYEGPKGGPGMR
                     EMLSTTAALYGQGMGDKVALITDGRFSGATRGFCVGHVGPEAAVGGPIALIRDGDVIT
                     LDGETGEISVALSDAELAERRKDWKPRETDHASGALWKYAQGVGDARHGAVTHPGGAA
                     EKHCYADI"
     gene            complement(2961..3365)
                     /locus_tag="PLQ11_10075"
     CDS             complement(2961..3365)
                     /locus_tag="PLQ11_10075"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010913220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRK25288.1"
                     /translation="MHRTLNPPGWAPAKGYANGVVARGTTVFLGGQIGWNAQQLFETD
                     DFVGQFEQTLVNILAVLKEAGAGPEHLVRMTWFITDKPAYTSRLKEIGAAYRRIIGRH
                     YPAMSVVVVAALIEDRARIEIEATAMLPDAAG"
     gene            complement(3365..5032)
                     /locus_tag="PLQ11_10080"
     CDS             complement(3365..5032)
                     /locus_tag="PLQ11_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019962584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRK25289.1"
                     /translation="MSGSGVTLSPSAHRDTFARDHLPPAAEWPDLLLDRPEFRYPDRL
                     NAARELTDRMVEKGFGDRTALIGNGRRRTYKELADWTNRLAHALVEDFGVTPGQRVLI
                     RSGNNPAMVAAWLAVTKAGAIAVNSMPMLRSGELAKVIDKGRITLALTDSRMAEELIA
                     AQALAPMLKRVVAFDGTSNHDAELDRAALDKPVTFACCDTAADDVALMAFTSGTTGEP
                     KGTCHFHRDLLIIADGYAREVLGVTPEDVFVGSPPLAFTFGLGGLAIFPLRFGAAAAL
                     LEDASPPNLIRYIEKYRATISFTSPTAYRVMLAAMEQGADLSSLRIAVSAGETLVPAV
                     FEAWTTKTGVPILDGIGATELLHIFISNRIGSALPGATGRPVSGYEARIVDADMKPVA
                     DGVPGLLAVRGPTGCRYLADARQRSYVRDGWNLTGDTFLRDADGVFHFVARADDMIVS
                     AGYNIAGPEVENALLKHPDVAECAVIGVPDAERGQIAEAHVVLKAGAIGDAAMVKRLQ
                     DHVKAVIAPYKYPRSIRFSDSLPKTATGKIQRFVLRQAAQDAAKGGA"
     gene            5303..>5535
                     /locus_tag="PLQ11_10085"
     CDS             5303..>5535
                     /locus_tag="PLQ11_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25290.1"
                     /translation="MTSKPGPARDLFGELEQAAQSLVRNDKARAEAALAKANRAVVDA
                     EQALKKAGVDAAAQDKFSGKTAGKPPAAAARPAS"
BASE COUNT          967 a         1914 c         1715 g          939 t
ORIGIN      
        1 ggtgcctcgc cggccggcgt ggcgcgggcg aatttgcggg cataggtctt ggccatgcgc
       61 cgctcgtcat cgctggccgc agtctcggcc ttgcgggtca tgtcgaaaat ccaggcgtcc
      121 ttgtgcggat cgcaggcatc gcgcgccagc agcatccagg acagccccag cggcgcctgg
      181 cgcggcactc cgttctggcc attgaacagg atctgcccca gcaccgcctg cgcgtaggga
      241 tggccttttt cggccgccag attgaaccag cgtgccgcct gccgtgcatc cttctcgcca
      301 tgcgcgccgt cgagatacat ccgcccgacg ttgtattgcg catccgggtc gccgaagtac
      361 gaggcggcgt agtggaacat ctcgtaggcg cggtccggat tggccttgac cggcgaaccg
      421 ggaatgccgt cgagataata cgaaccgagc tgcacgaacg ccttggcgac aaccccggcc
      481 ttcatcgtac cgcgcacctc ttcggcattg gcattggcga tctgctgata cagccggaat
      541 gcccggtaat cgtcctgctc gacaccgtct ccctcggcat acatgcggcc aagcttgaac
      601 tgggcggcga tatcgccctt ggtggcggcg tagttcagcg ccttggccgc gcccggcttg
      661 tcgccggcat tgaggccgcg cacgccggcc ttcagcgcat cggtgaccga cttgaacgat
      721 tcgatcggcg gagccttgcc ctccggctgg gcattggcat ccagcgcctc agccggcccg
      781 gcaaggcagc agacgcacac gagcatcgca aagggaaaac ggtcagatat cggcatagca
      841 atgtttctca gccgcaccgc ccgggtgcgt cacggcgccg tggcgggcat cacccacccc
      901 ttgcgcatat ttccacagcg cacccgatgc gtgatcggtc tcgcgcggtt tccagtcctt
      961 gcggcgctcg gccagttccg catcgctcag cgcgacggaa atctcgccgg tctcgccatc
     1021 gagggtaatc acatcgccgt cacggatcag tgcgatcggc ccgccgaccg ccgcttccgg
     1081 cccgacatgg ccgacgcaga agccgcgcgt cgcgccggag aagcgcccgt cggtgatcag
     1141 cgccaccttg tcgcccatcc cctgcccgta gagcgcggcg gtggtcgaca gcatctcgcg
     1201 catgccaggt ccgcccttcg gcccttcata gcggatcacc agcacctcgc cttccttgta
     1261 ggcgcgcttt gacaccgcct cgaaacaggc ttcctcgctg tcgaagcaac gcgccgggcc
     1321 ggtgaatcgc agcaccttca tgcccgcaac cttcacgatg gccccgtcag gcgccagatt
     1381 gccggacagg ccgacgaccc cgccagtcgg cgtgatcggc ttgttggcag gccgcaccac
     1441 atcctgatcg tcgttccagc gcacggattc gaggttctcg gcgatcgtcc ggccggtcac
     1501 ggtcaggcaa tcgccattga gatagccgtg atcgagcagc gtcttcatca gcagcggaat
     1561 gccgcccacc tcgaacatat ccttggcgac atagcgcccg ccgggcttga ggtcagcgat
     1621 atagggggtg cgcttgaaga tctcggcgac gtcgaacagg tcgaacgcaa tgccgcattc
     1681 atgcgcgatc gccggcagat gcagcgccgc gttggtcgag ccgcccgagg cggcaaccac
     1741 ggtcgcggca ttttccagcg cctcgcgggt aacgatatcg cgcggccgga tgttcatggc
     1801 gatcagctcc atgatcatct cgccggcggt catgcagaac cggtcgcgga tttcatacgg
     1861 cgccggcgca ccggccgaat agggcagcgc cagcccgatc gcctcggaca cggtggccat
     1921 ggtattggcg gtgaactgcg ccccgcacgc ccctgccgag ggacaggcaa cctgctcgat
     1981 ctcctcaagg tcagcatccg acatctcgcc gaccgaatgc ttgcccaccg cctcgaacat
     2041 gtcctggatc gtcaccggac ggccgcggaa agtgccgggc aggatcgaac cgccatagat
     2101 gaagatcgac ggcacattca gccggcacat cgccatcatc atgccgggca gcgacttgtc
     2161 gcagccggca agcccgacca gcgcatcata gcaatggccg cgcatggtca gttccaccga
     2221 atccgcgatc acctcccgcg aggccagcga ggacttcatg ccctgatggc ccatcgcgat
     2281 gccatcggtg acggtgatgg tgcagaattc gcgcggggtc ccgccggccg aggcaacgcc
     2341 cttcttgacc gcctgcgcct gccgcatcag cgcgatattg cagggtgcgg cctcgttcca
     2401 gcacgaggcg acgccgacga acggctggtg aatctggtcc ttggtgaggc ccatggcgta
     2461 ataataggag cgatgcggcg cgcgcgccgg cccttccgtc acatgacggc tcggcaatct
     2521 cgacttgtcg gtcacccgct tgtccatgcc cgttccaaac tcccgccaca gtcccacatg
     2581 acgcctaacg tcgcctcaac ggcagcgccc gaccgcaagg atcagccgcg tcctgtgcct
     2641 gtggatccga aaacgctccg tccgcccgaa cgtccgggct ccgatggcgc tttgcctagg
     2701 cgcaactgct ctgcccgaaa agtggcagca gcgcggcaca attgggcagg ttttggcgct
     2761 gattgtttat tgttgccggt gttgcggcga tgtcacaaac cacgcatcgg ccggttgcag
     2821 aagcaagcgc cgcgatgcgc cgcaccatgg cctggatcac gctctcgttt cggttgaatc
     2881 accgaaacat aggcaacggg atcgatttca agtggttaga gcatgctgcg aaaaccgcct
     2941 acactttttc gcagcatgct ctatcccgcc gcatcgggca gcatcgccgt tgcctcgatc
     3001 tcgatcctgg cgcgatcctc gatcagcgcg gcaacaacca ccaccgacat ggccggatag
     3061 tgccgcccga tgatccgccg gtaggccgcg ccgatctctt tcagccggct ggtataggcc
     3121 ggcttgtcgg tgatgaacca ggtcatccgc accagatgtt ccgggccggc gccggcctcc
     3181 ttcagcacgg ccaggatatt gaccagcgtc tgttcgaact ggccgacgaa atcgtcggtc
     3241 tcaaaaagct gctgcgcgtt ccagccgatc tggccaccga gaaagaccgt cgtgccgcgc
     3301 gcaacgacac cgttggcata gcctttggcc ggcgcccatc cgggcgggtt caatgtccgg
     3361 tgcatcaggc gcctcccttg gccgcatcct gcgccgcctg ccgcaggacg aaacgctgga
     3421 tcttgcccgt cgccgtcttg ggcaggctgt cactgaaccg gatcgaccgc ggatatttgt
     3481 agggcgcgat gactgccttc acatggtcct gcaagcgctt gaccatcgcc gcatcgccga
     3541 tcgccccggc cttgagcacc acatgcgcct cggcgatctg tccgcgctcg gcgtccggca
     3601 cgccgatgac ggcgcattcg gccacatcgg gatgcttgag cagcgcattc tccacctcgg
     3661 gcccggcgat attgtagccg gccgaaacga tcatatcgtc ggcgcgggca acgaaatgga
     3721 acaccccgtc cgcatcgcgc aggaaggtat cgccggtcag gttccagccg tcgcgcacat
     3781 agctccgctg gcgcgcatcg gccagatagc ggcatccggt cggcccgcgc acggcgagca
     3841 ggcccggcac gccatcggca accggtttca tgtcggcatc gacgatgcgg gcctcatagc
     3901 cgctcaccgg acgaccggtc gcgcccggca gcgcggagcc gatacggttg gaaatgaaga
     3961 tgtgcagcag ttcggtcgcg ccgatcccgt caaggatcgg cacgccggtc tttgtcgtcc
     4021 acgcctcgaa caccgcgggc accagcgtct cgccggccga gaccgcgatg cgcagcgagg
     4081 acaggtccgc cccctgctcc atcgccgcca gcatcacccg gtaggcggtg ggcgaggtga
     4141 acgagatcgt cgcgcggtat ttctcgatgt agcggatcag attgggcggc gaggcatcct
     4201 ccagcagcgc cgccgccgcg ccgaacctga gcgggaagat cgccagcccg cccagcccga
     4261 aggtgaaggc cagcggcggc gagccgacaa acacatcctc aggcgttacg cccagcacct
     4321 cgcgcgcata gccatcggca atgatcagca gatcgcgatg gaaatggcag gtgcccttcg
     4381 gctcgcccgt cgtgcccgag gtgaaggcca tcagcgccac atcgtcggcg gcggtatcgc
     4441 agcaggcaaa ggtcaccggc ttgtcgagcg cggcgcggtc cagttcagcg tcgtgattgg
     4501 acgtgccgtc gaaggcaacc acccgtttca gcatcggcgc cagcgcctgg gcggcgatca
     4561 gttcctcggc catgcggctg tcggtgagcg ccagcgtgat gcgtcccttg tcgatgacct
     4621 tggcgagttc gccggaacgc agcatcggca tcgaattgac ggcgatcgcc ccggccttgg
     4681 tgaccgccag ccaggccgcc accatcgccg ggttgttgcc cgagcggatc agcacccgtt
     4741 gtccgggagt gacaccgaaa tcctcgacca gcgcatgcgc cagacgattg gtccagtccg
     4801 ccagttcctt gtaggttcgc cttctcccgt tgccgatcag cgccgtgcgg tcgccaaagc
     4861 ccttctcgac catgcggtcg gtcagttcgc gcgccgcatt cagccggtcc ggataacgga
     4921 actcgggccg gtcgagcagc aggtccggcc attctgccgc cggcggaaga tgatcacgcg
     4981 cgaatgtgtc ccgatgcgcc gatggcgaca gcgtaacccc agagccagac atgtcccctc
     5041 cccgatccgt ccgccggacc atcggcggca aggcgccgta tccgattgcc tgagcacggt
     5101 aggtatttta agattaaaat aagctggcag gaccgcgcca agctgtcaac gccccaggcg
     5161 atcagaggcg gtctggcggg cgccggccgg cccttggcgg tcctttcccc ttggcttttc
     5221 cccaagcgcg gcaaccgctt gcccgttccg ccctcagcca tgccatactt gcgcgcatgc
     5281 gaatcactga gcccgaggcc ccatgacgtc gaaacccgga cccgcgcgcg acctgttcgg
     5341 cgaattggag caagcggccc agtcgctggt cagaaacgac aaggcgcgcg ccgaggctgc
     5401 gctggccaag gccaatcgcg cagtggtgga tgccgaacag gccttgaaga aagccggtgt
     5461 tgacgcggcc gcgcaggata aattttccgg caaaaccgca ggcaagccgc cggccgccgc
     5521 cgcaaggccg gcctc
//
LOCUS       DAOMXG010000157         5496 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_7851_length_5496_cov_9.374380, whole genome shotgun sequence.
ACCESSION   DAOMXG010000157 DAOMXG010000000
VERSION     DAOMXG010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 5496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5496
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7851_length_5496_cov_9.374380"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..1643
                     /gene="hisS"
                     /locus_tag="PLQ11_10090"
     CDS             138..1643
                     /gene="hisS"
                     /locus_tag="PLQ11_10090"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HRK25291.1"
                     /translation="MTAETAPKKPKDLEARIAKGFVDRDANDIAATEAMMAKIREVYE
                     LYGFAAVETPFIEYTDALGKFLPDQDRPNAGVFSFQDDDDQWLSLRYDLTAPLARFYA
                     ENHERLPKPYRSYRAGWVFRNEKPGPGRYRQFMQFDADTVGAASVAADAEICMMAADT
                     LEALGIKRGDYVIKVNNRKVLDGVMEAAGVREDQKLTVLRAIDKADKFPPEEIKKLLM
                     EGRKDESGDFTKGAGLSEEGAAFVLLTLSLSGRADGAPSTRFAGLIERLSGTVGVGGF
                     VELDSIEALVRAAGYGPDRIRIDPSVVRGLEYYTGPVFEAELTFEVKGEDGRPIRFGS
                     VGGGGRYDGLVSRFLPEPAPATGFSIGVSRLLAALKAVNSPIVAAAAKPGPVVVVAMD
                     KDAAAMAGYQQLVSTLRKAGIAAEMYLGSAGMKPQMKYADRRGSCCVVIQGSNERNAG
                     VVQIKDLVMGATITGIKDRAEYLAAQAEAQFTVPVADLVAGVRRVLARRSG"
     gene            1731..2810
                     /locus_tag="PLQ11_10095"
     CDS             1731..2810
                     /locus_tag="PLQ11_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HRK25292.1"
                     /translation="MTKTSPIAALNALFQRHGFAPSDVPILQPADPFIELSGEDIRRR
                     LFLVSDGGETELCLRPEFTIPAARAYLAAGARGARASAFIGPVFRQRTDGPGEFIQAG
                     LESFGRSDIEAADAEMLALAIEAEALLDGGGRTIRIGDVGLMTSLIASLGLSPALARR
                     LRQHGLGAQAAPSLANGLAAYQGVLNALKGADPTEARAFVKDLLSIAGLKAVGGRTPA
                     EIAERFLEKADAASSSLSGEQAEIFRRFAAISGDPDTVSAEVRALSKSAGLSLEAAVD
                     RFDARTGFMAARGVDLGRLTAATTFNRNLDYYSGFVFEFVARAGERPAIAGGRYDGLL
                     RRLGADADCPAVGCAIWLERFGSVA"
     gene            2807..3802
                     /gene="hisG"
                     /locus_tag="PLQ11_10100"
     CDS             2807..3802
                     /gene="hisG"
                     /locus_tag="PLQ11_10100"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HRK25293.1"
                     /translation="MSGSSLVIGVPSKGRLQENTFAFFARAGMPLSQARGSRDYRGQI
                     GGIAGAEIAFLSASDIAGELARGAIHLGVTGEDLLAEAVPDREEKIVMLTPLGFGHAN
                     VVVAVPEAWIDVRSMADVEDVAAAFHARHHRRMRVATKYINLTRRHFAQHGIVDYRIV
                     ESLGATEGAPAAGTAEMIVDITTTGATLAANGLKVLDDGTMLKSEANLVAAVAADWSP
                     AAREAARQVLLRISAEETARTTREVRTRLDRPVDAAALAAFHAVLPYGAEAGEVILHC
                     PAGQVGALADWLTRNGATTVTVSAQAYVFQAANPLFERLEHAAIKWKPVNRQSML"
     gene            3918..5015
                     /locus_tag="PLQ11_10105"
     CDS             3918..5015
                     /locus_tag="PLQ11_10105"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRK25294.1"
                     /translation="MPSLNRRSLLSVAAASLAAPQLSRPAHAADLDRLVGPLLMLGFH
                     GANAEAAGAQTLARHIASGAAGGACFLGHNTRSKAGIEGLTALFRNAATQRPPLLSVD
                     QEGGVVQRLSSKIGYLDTPGATDIAERGEAQALAIYERMARMMRGAGFNLNLAPVVDL
                     GFERRNPIIARHGRAYGDDGTTVARYAGAFCQAHRRVGVLTALKHFPGHGSTVIDSHK
                     APVDLTETWREDELEPYRQLVARGLVDIVMSGHLSHARMGGAGVAATLSKAAIEGVLR
                     KQCGWGGVVMTDDVDMAAIRSTLAPPEAAVAALAAGNDIVLMSNSAAPDAELPRRVTA
                     AIRQALADGRLTMARLEQANARVISLMEKTS"
     gene            5012..5398
                     /locus_tag="PLQ11_10110"
     CDS             5012..5398
                     /locus_tag="PLQ11_10110"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HRK25295.1"
                     /translation="MRGAIVGLALVAGSALALAVAFPYGVAFKAESLGGMPFDPVALP
                     TLKVVANGETSGSTGCNRYFGKIAIKGDAVAVGDLAMTRMACFDGAGTNERRFAAALG
                     SAKAWRFDGSRLLLETAQGVLVLVRQ"
BASE COUNT          917 a         1722 c         1824 g         1033 t
ORIGIN      
        1 cctgcctctc ccctcgccct aaccctctcc ccgcaaagcg gggcgagggg acgcgcgctc
       61 tccacccgcc acgcaccatg acgcttgccc ttgccggcca tcccctttaa tgccgcgggc
      121 aacagaaagt tgccaccatg accgccgaaa ctgcgccgaa aaagccgaaa gaccttgaag
      181 ctcgcatcgc caagggcttt gtcgatcgcg atgccaatga catcgccgca accgaggcga
      241 tgatggcgaa aatccgcgag gtctatgaac tctacggctt tgcggcggtg gagaccccgt
      301 tcatcgaata caccgacgcg ctcggcaagt tcctgcccga tcaggaccgg cccaacgccg
      361 gtgtcttttc gtttcaggac gatgacgacc agtggctgag cttgcgctat gacctgacgg
      421 cgccgctcgc ccgcttctat gccgagaacc acgagcggct gccgaaaccc tatcgcagct
      481 atcgcgccgg ctgggtgttc cgcaacgaaa agccggggcc gggccgctat cgccagttca
      541 tgcagttcga tgctgatacg gtcggcgcgg cctcggtcgc ggcggatgcc gagatctgca
      601 tgatggcggc cgataccctg gaggcgctgg gcatcaagcg cggcgactac gtcatcaagg
      661 tcaacaaccg caaggtgctc gatggggtta tggaagcggc cggcgtccgc gaggaccaga
      721 agctcactgt gctgagggcc atcgacaagg ccgacaagtt tccacctgag gaaatcaaga
      781 agcttctgat ggaaggccgc aaggatgaaa gcggcgattt cacgaagggg gcggggcttt
      841 cagaggaagg tgctgcgttt gtcctgctga cgctgtcgct ctccggacgc gccgatgggg
      901 cgccgtcgac ccgatttgcc ggattgatcg aaaggctttc aggcacggtt ggtgttggag
      961 gctttgtgga acttgactcc attgaagccc tcgtccgcgc cgccggctac ggcccggacc
     1021 gcatccgcat cgacccttcc gtcgtccgcg gcctcgaata ttacaccggc ccggtgttcg
     1081 aggcggaact caccttcgag gtgaagggcg aggacggcag gccgatccgc ttcggttcgg
     1141 tcggcggcgg cgggcgttat gatggcctcg tctcgcgctt cctgccggaa cccgcgccgg
     1201 ccaccggctt ttccatcggc gtctcccgcc tgctggctgc actgaaggcg gtcaattcgc
     1261 ccatcgttgc cgctgccgca aaacccggcc ccgtggtggt ggtggccatg gacaaggatg
     1321 ccgcggcgat ggccggctat cagcagcttg tcagcacgct gaggaaagcc ggcattgccg
     1381 ccgagatgta tctcggctcg gccggaatga agccgcagat gaaatatgcc gaccggcgcg
     1441 gctcctgctg tgtcgtcatc cagggctcca atgaacggaa cgccggcgtg gtccagatca
     1501 aggatctcgt catgggcgcg accatcaccg gcatcaagga ccgcgccgaa tatctcgccg
     1561 cgcaggccga agcccagttc accgtgccgg tcgccgatct tgtcgccggt gtgcggcgtg
     1621 tacttgcccg gcgcagcggc tgagccgctg ttcagcacgt tttcgattcc ccatggccgc
     1681 gcgccgcctg tttctgctag agagcccgca cacagcaaga gttgccgccc gtgaccaaga
     1741 cgtctcccat cgctgccctg aatgcgctat tccagcggca tggttttgct ccgtctgatg
     1801 tgccgattct gcagccggcc gatccgttca tcgaattatc gggcgaggat attcgccggc
     1861 ggctgtttct ggtcagcgac ggtggcgaga ccgagctgtg cctgaggccg gaattcacca
     1921 ttccggcggc gcgggcctat cttgcggccg gggcgcgtgg cgcgcgggct tcggctttca
     1981 tcgggccggt atttcgccag cgcaccgatg ggccgggcga attcatccag gctggtctgg
     2041 aatccttcgg ccgcagcgac atcgaagcgg ccgatgccga gatgctggcg ctcgccatcg
     2101 aggccgaggc gctgctcgat ggcggagggc ggaccatccg catcggcgat gtcgggttga
     2161 tgacatcgct gattgcctcg ctcggcctgt cgccggcact ggcacgccga ttgcgccagc
     2221 atggccttgg cgctcaggca gcaccatcgt tggccaatgg tctggcggcc tatcaggggg
     2281 ttctcaatgc gctcaagggc gccgatccga ccgaggcgcg agccttcgtc aaggacctgc
     2341 tatcgatcgc tggcttgaag gcggtcggcg ggcgcacgcc ggctgagatc gccgagaggt
     2401 ttcttgaaaa ggccgatgcg gcgtcgagtt cgctcagcgg cgagcaggcg gagattttcc
     2461 gccgctttgc cgccatttcc ggcgatcccg atacggtcag cgccgaagtg cgggcactgt
     2521 cgaagtctgc gggcctgtcg ctggaggcgg cggttgaccg gttcgatgca cgaaccggct
     2581 tcatggcggc acgcggcgtc gacctcgggc ggttgactgc ggcaaccacc ttcaaccgca
     2641 atctcgacta ttattccggc ttcgtcttcg aattcgtggc gcgggccggc gaacgcccgg
     2701 cgatcgctgg cggacgctat gacggattgc tgcggcggct cggcgccgat gccgattgcc
     2761 ccgcggtggg gtgtgcgatc tggctcgagc ggttcgggag cgtggcatga gcggatcgtc
     2821 ccttgtcatc ggtgtgccct caaaagggcg actgcaggaa aacactttcg cctttttcgc
     2881 ccgggccggc atgccgctga gccaggcgcg cggctcccgt gactatcgcg gccagatcgg
     2941 tggaatagcc ggggcagaga tagcctttct gtccgcctcc gacattgccg gcgaactggc
     3001 gcgtggcgcg atccatctcg gtgtgaccgg cgaggacctg ctggccgagg cggttcccga
     3061 ccgcgaagag aagatcgtca tgctgacccc gctcggcttt ggccatgcca atgtcgtggt
     3121 ggcggtgccg gaggcctgga tcgacgtccg ctcgatggcg gatgtcgagg atgtggccgc
     3181 cgcctttcat gcgcggcatc accgccggat gcgggtcgca accaaataca tcaacctgac
     3241 gcggcgccat ttcgcccaac atggcatcgt tgattaccgc atcgtcgaga gcctcggcgc
     3301 gaccgaggga gcaccggccg ccggcacggc agagatgatc gtcgacatca ccaccacagg
     3361 cgccacgctg gctgccaacg ggctgaaagt gctcgacgat ggcacgatgc tgaaatccga
     3421 ggccaatctg gttgcggcgg tggcggctga ctggtcgccg gcggcgcgcg aggcggcgcg
     3481 gcaggtgctg ttgcgcatca gcgccgagga gaccgcacgg acgacgcgtg aagtgcgcac
     3541 gcggctggac cgccccgttg atgcggccgc gcttgccgcc tttcacgctg tgctgcccta
     3601 tggggcggag gcgggcgagg tgatcctgca ttgcccggct ggccaggtcg gggccttggc
     3661 cgattggctg acgcgcaacg gcgccacaac tgtcacagtt tcggcacaag cctatgtctt
     3721 tcaggccgcc aacccgctat tcgagcggct agagcatgct gcgattaagt ggaagccggt
     3781 taatcgccaa agcatgctct aaactattga taacgatcac gctctcgttt tgatgattcc
     3841 atcaaagcga gagcgtgatc tgacgcagcg aatcggctga agcagcgccg gttcgcatct
     3901 gcgggatcgg atctgacatg cccagtttga atcgtcgttc tcttctgtcg gttgccgccg
     3961 ctagcctggc ggctccgcag ctttcgcgtc ccgctcatgc cgccgatctt gatcgcctgg
     4021 tcggcccgtt gctgatgctc gggttccatg gcgccaacgc cgaggcggct ggcgcccaga
     4081 cgcttgcccg ccatattgcc tccggtgccg ctggcggcgc ctgctttctt ggtcacaaca
     4141 ccaggtcgaa ggcaggaatc gaagggctca cggcgctgtt ccgcaatgcg gcgacgcagc
     4201 ggccgccgct gctctcggtg gaccaggaag gcggcgtcgt gcagcgattg tcgtccaaga
     4261 tcggctatct cgacacgccc ggtgcgactg atattgccga aaggggcgag gcgcaggcgc
     4321 tggcgatcta cgagcggatg gcgcggatga tgcgcggcgc aggcttcaac ctcaacctcg
     4381 cgcctgtcgt cgatctcggc tttgaaaggc gtaacccgat catcgccagg catggacgcg
     4441 cctatggcga cgatggcacg acggttgcgc gctatgccgg ggcgttctgc caggcacacc
     4501 gccgcgttgg cgtgctgacg gcgctcaagc attttcccgg acacggctcg accgtgatcg
     4561 acagtcacaa ggcaccggtc gacctgaccg agacctggcg cgaggatgaa ctcgaaccct
     4621 atcgccagtt ggtcgcgcgc ggcctggtcg atatcgtgat gagtggccat ctctcccatg
     4681 cccgcatggg cggggcgggt gtcgccgcca ccttgtcgaa ggcggcaatc gaaggtgttt
     4741 tgcgaaagca atgcggctgg ggtggcgttg tcatgacaga cgatgtcgac atggcggcca
     4801 tccgctcgac actcgcaccg cccgaggcgg ccgtcgcggc gctggcggcc ggcaatgata
     4861 tcgtgctgat gtccaattct gccgcgccgg atgcggaatt gccccgccgg gtcacggcgg
     4921 cgatccgtca ggcccttgcc gatggccgcc tcaccatggc tcgcctggag caagccaatg
     4981 cacgggtcat cagcctcatg gagaaaacct cttgagaggc gcaatcgtcg gacttgctct
     5041 ggttgccggc agtgcgttgg ccttggcggt cgcgttcccg tatggcgtgg cattcaaggc
     5101 cgagagcctg ggaggcatgc cgtttgaccc tgtggcgctg ccgacgctga aggttgtggc
     5161 caatggcgag acgtcgggct caaccggatg caacaggtat ttcggcaaga ttgcaatcaa
     5221 gggcgatgct gtggcggtcg gtgatcttgc catgaccagg atggcctgtt tcgatggtgc
     5281 cggcaccaat gaacgccgct ttgcggcagc cctcggatcg gccaaggcct ggcggttcga
     5341 tggatcacgc ctgctgctcg aaaccgcgca gggcgtcctg gtgctggtca ggcagtagag
     5401 catgctgcga aaaaagtgta ggcggttttt tgcttattgc atgctctagg ctgaatcgac
     5461 attcaacgca tccatagaat tgctgcggcg agttgc
//
LOCUS       DAOMXG010000158         5454 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_7931_length_5454_cov_8.665308, whole genome shotgun sequence.
ACCESSION   DAOMXG010000158 DAOMXG010000000
VERSION     DAOMXG010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 5454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5454
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7931_length_5454_cov_8.665308"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1597)
                     /locus_tag="PLQ11_10115"
     CDS             complement(<1..1597)
                     /locus_tag="PLQ11_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRK25296.1"
                     /translation="MLDIRDLTFRLGDRLLFDHASCALPPGGRIGFVGRNGSGKTTLF
                     RMIAGELGAESGTIQLPSGVRIGRVEQEAPGGPQTLLDFVLAADTERAALLAEAEIAR
                     EPERIADIHLRLADISAQSAPARAGAILHGLGFDTAAQNRPCSDFSGGWRMRVALAAT
                     LFVEPDLLLLDEPTNYLDLEGTIWLQDHLTRYPHTMIAVSHDRDFIDTIATHTLHIDA
                     CKLVLYRGGYSQFERQRSEAARLAEKAAASVAAERARLTAFIERFRAKASKAAQAQSR
                     ARRLEKLADVSLFAGEQAARLTIPDPEKPQAPPLVAFDRVAAGYGETIVLKRLSLSIG
                     DEDRIGLIGANGNGKSTFVKLIAGRLAPLSGNIARSPKLNVAYFAQHQLEELDADATP
                     YLLVRRRMEGQPEAKIRARAAAFGFSSDKSDTPITKLSGGEKARLLFGLATFDGPHLV
                     ILDEPTNHLDIPMRDALVEALAAYKGAAIIVSHDRHLIDATCDRLWLVDKGNVAPFDG
                     DLSDYARLVMNARASSDDTPRERT"
     gene            1737..2420
                     /locus_tag="PLQ11_10120"
     CDS             1737..2420
                     /locus_tag="PLQ11_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HRK25297.1"
                     /translation="MNKLIAEVFGTAVLVFVGCGSVALTGNGGAMPLGILPIALAFGL
                     SVTAMAYGIGPVSGCHINPAVTAAVWSAGRMDTPEAIRYIIAQFIGGFVGAGLLAVVL
                     MGNAKGYNLATSGLGQTTFANYSVASAFIVEMVATMIFTVTILGVTQKNGGGNVAGLV
                     IGLTLALLHLAFVPVTGNSLNPARTFAPNLYVGGQAAAQIWLYMIAPMVGGVIAGFLF
                     RSGLFNADK"
     gene            2594..3289
                     /locus_tag="PLQ11_10125"
     CDS             2594..3289
                     /locus_tag="PLQ11_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HRK25298.1"
                     /translation="MKHSMPDGMPAPLSRGARLRSYFLTGLVVAGPLAITVYLSWWFI
                     NLIDGWVRPLVPAAYWPESYLPFRLPGFGVIILLFGLTLLGFLTANLVGRTLLDYGEG
                     LVSRMPIVRGLYKSAKQIFETIFSQSGTSFRRAGLIQYPGPGLWSIVFLSGAPSGDIA
                     AKLPGQGEYLSVFLPCAPNPTTGFFLYVRRSEVIELQTSVDDAFKIVLSLGLLKEEDQ
                     QAQLAKLARQDPA"
     gene            complement(3350..3679)
                     /gene="hspQ"
                     /locus_tag="PLQ11_10130"
     CDS             complement(3350..3679)
                     /gene="hspQ"
                     /locus_tag="PLQ11_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004681888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HRK25299.1"
                     /translation="MAVRQAKYRIGQVVRHRIYPFRGVVFDIDPEFANTEEWWESIPQ
                     EVRPRRDQPFYHLYAENDETEYVAYVSEQNLVPDTSSEPLRHPQIKDVFVRSSDGSLH
                     AKTRMMH"
     gene            4086..5048
                     /locus_tag="PLQ11_10135"
     CDS             4086..5048
                     /locus_tag="PLQ11_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HRK25300.1"
                     /translation="MIDVINLALPFFGLILLGFFCGRLMKMPEAGLAWMNFYIVYVAL
                     PPLFFKLIVATPFAELQNWSFVVGTTLSTYLVFVLSLAIGLAFYRGDLKPATLQAVLG
                     AYSNIGYMGPGLTLAAIGPKAAAPTALIFVFDNILLFSLIPFLMALGGTEKLDVARTA
                     RMVLWKIVSHPFNIAAFVALIFAYFQLKPPMAIDRMLTMLYGSAAPVALFALGLTVAS
                     RRVTRVPVELPIHLCVKLFLHPAIILLLLSLLGPFEKEWVYSAVLMASLPPALNVFVV
                     ARQYDVYVQEASTAILLGTLASIVSLTFVLYLITTGALPISLFG"
     gene            complement(5115..>5454)
                     /locus_tag="PLQ11_10140"
     CDS             complement(5115..>5454)
                     /locus_tag="PLQ11_10140"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HRK25301.1"
                     /translation="AGEALHAFPPVGAQGMNLGFRDARSLVAAIRSAHERGEDVGSAA
                     ALAPYERSRQIDAVSRTYGVDLLNRSLISGLLPLDLARFAGLTAAAHVGPIRRMLMRA
                     GMGRSPIAAA"
BASE COUNT          898 a         1701 c         1756 g         1099 t
ORIGIN      
        1 cggtccgctc gcgcggggtg tcgtcggaac tggcgcgggc attcattacc agccgcgcat
       61 aatccgacag atcgccgtca aacggcgcga cattgccctt gtccaccagc cacagccggt
      121 cacaggtggc atcgatcaga tggcggtcgt ggctgacgat gatcgccgcg cccttgtagg
      181 cggccagcgc ctcgaccagc gcatcgcgca tcggaatgtc gagatggttg gtgggctcgt
      241 cgaggatgac cagatgcggg ccatcgaagg ttgccagccc gaacagcagg cgggccttct
      301 cgccgcccga aagcttggtg atcggagtat cggacttgtc ggaggaaaag ccgaaggccg
      361 cagcgcgcgc ccggatcttc gcctccggct gcccttccat ccgccgccgc accagcagat
      421 acggcgtggc atcggcatca agctcttcaa gctggtgctg ggcgaaatag gcgacgttga
      481 gttttggcga gcgggcgatg ttgcctgaca ggggagccag tcgcccggca atcagcttga
      541 cgaaggtgga cttgccgttg ccgttggcgc cgatcaatcc gatgcgatcc tcgtcgccga
      601 tgctcagcga caggcgcttg agcacgattg tctcgccata tccggcggcc acccgatcga
      661 aggcaacgag cggcggcgct tgcggcttct cggggtcggg aatcgtcagc cgggccgcct
      721 gttcaccggc gaacaggctg acatcggcga gcttttccag ccgccgcgcc cggctctggg
      781 cctgggccgc cttggatgcc ttggcgcgga accgctcgat gaaggcggtc agccgcgccc
      841 gttcggcggc aacggaggcg gcggcctttt ccgccagccg cgccgcttcg gaccgctgcc
      901 gctcgaactg cgaatagccg ccgcgataca gcaccagctt gcaggcgtcg atgtgcaggg
      961 tatgggtggc gatcgtatcg ataaaatcgc ggtcatggct gacggcgatc atcgtatggg
     1021 gatagcgcgt caggtgatcc tgcagccaga tcgtcccttc aaggtcgaga taattggtcg
     1081 gctcgtcgag cagcagcagg tccggctcga caaacagtgt cgcggccagc gccacccgca
     1141 tgcgccagcc accggaaaaa tcggagcacg ggcgattctg cgctgccgta tcgaagccga
     1201 ggccgtgaag gatcgccccg gcccgcgccg gtgccgattg ggcggaaatg tctgcgaggc
     1261 gcagatggat gtcggcgatc cgctcgggct cgcgcgcgat ttcggcttcg gccagcagcg
     1321 ccgcgcgttc ggtatcggcg gcgagcacga aatccagcaa cgtctgcggg ccgcccggtg
     1381 cttcctgttc gacccggccg atccgcacgc cgctcggcag ctggatcgtc ccggattcgg
     1441 cgccaagctc cccggcgatc atccggaaca gcgtggtctt gccgctgccg ttgcggccga
     1501 caaagccgat gcgtccgccc ggcggcaggg cacaggaggc gtggtcgaac aggagacgat
     1561 cgccaaggcg aaacgtcaga tcacggatgt ccagcatgcc gccttttggc tgcgaaacag
     1621 aatttctgca agccgccgtt catattccgg ttgctaacca gcctcggggg cttgacgggc
     1681 gcggcgatgt aatgctcatt cgcgccaata cgactttggt cgagcgggga aagacgatga
     1741 acaagttgat tgctgaagta ttcgggacgg ctgtcctggt ttttgtcggc tgcggctcgg
     1801 tggcgctgac cggcaatggt ggcgccatgc cgctgggtat tctgccgatt gcgcttgctt
     1861 tcggtctttc ggtgacggcc atggcctacg gcatcggtcc ggtgtcgggt tgccacatca
     1921 atccggccgt gacggcggcg gtctggtctg ccggccggat ggatacgcct gaggctatac
     1981 ggtatatcat tgcccagttc atcggcggct ttgtcggcgc cggcctgctg gcagtggtgc
     2041 tgatgggcaa tgcaaagggc tacaatctgg cgacgtccgg tctcggccag acgaccttcg
     2101 ccaattattc ggtggcgtcc gcgttcatcg tcgagatggt ggcgacgatg atcttcaccg
     2161 tcacgatcct gggtgtgacg cagaagaacg gtggcggcaa tgtcgccggt ctggtcatcg
     2221 gcctgacgct ggcgctgctg catctggcct tcgtgccggt gaccggcaat tcgctgaacc
     2281 cggcccgcac cttcgcgccg aacctttacg tcggcggcca ggctgcggcc cagatctggc
     2341 tttacatgat cgccccgatg gtcggtggcg tgatcgccgg cttcttgttc cgcagcggcc
     2401 tgttcaacgc ggacaagtaa tcggtccggt tatccggccg cgaaagcccc gccacgagcg
     2461 gggctttctt tttgtagccg gcagtaccgc tggtccagcc agcacgaaat cccgccggcc
     2521 cggagcgatt gccgcttttc atcggcgtcc cgcttgctta tgagcaagcg atgagcagct
     2581 cagggtcagg catatgaagc attcgatgcc cgatggcatg ccggcgccgc tgtcgcgggg
     2641 cgcgcggctg cgcagttact ttctgaccgg tcttgtggtg gccgggccgc tggcgatcac
     2701 cgtctatctg tcctggtggt tcatcaacct gattgacggc tgggtgcggc cgctggtgcc
     2761 tgccgcctat tggccggaga gctatctgcc gttccgcctg ccgggtttcg gcgtcatcat
     2821 cctgctgttc gggctgacgc tgctgggctt tctgaccgcc aatcttgtcg gacgcacctt
     2881 gcttgattac ggcgaggggc tggtcagccg catgccgatc gttcgcgggc tctacaagag
     2941 cgccaagcag attttcgaga cgatcttttc ccagtcggga acctcgttcc gccgcgccgg
     3001 cctgatccag tatccggggc ccggcctgtg gtcgatcgtg ttcctgtcgg gggcgccctc
     3061 cggcgacatt gccgccaagc tgccggggca gggggaatat ctcagcgtgt ttttgccctg
     3121 tgcgcccaac ccgaccaccg gcttctttct ctatgtccgg cgttcggagg tgattgaatt
     3181 gcagacgtcg gtagacgatg ccttcaagat cgtgctgtcg cttggcctgc tcaaggaaga
     3241 ggaccagcag gcgcaactcg ccaagctggc tcgccaagac cccgcctgaa acgacacttc
     3301 gggtctggcg cttcggcctg aagatcccgg tcgcggccgt ccggccacat cagtgcatca
     3361 tgcgcgtctt ggcgtgcagc gaaccgtcgg aagagcggac gaacacgtcc ttgatctgcg
     3421 gatggcgcag cggctcgctg gatgtgtccg gaaccaggtt ctgctcggac acataggcga
     3481 catattcggt ttcgtcattc tcagcgtaga gatggtagaa cggctggtcc ctgcgcggcc
     3541 gcacttcctg cgggatcgac tcccaccatt cctcggtatt ggcgaattcc gggtcgatgt
     3601 cgaacaccac gccgcgaaac gggtagatgc ggtggcggac aacctgaccg atacgatact
     3661 tggcttggcg aacagccatg gccggacact ccggggctcg gggcgcggct gatactcctg
     3721 ccgacgctct cccgtcaaga gaggaactct atcgcaactg ccccgcgccg aaaagaccat
     3781 gcacgggcgc ccggttggtc attcccggcg cctcgggcgt cgtgccgcac cgatttttgc
     3841 tggacccata ctgccggcgc tgttacctca gccagatcgt ctgcgcattt tcaagcgaag
     3901 tggaatccgg tgcgcgtgaa ggaaatgcgc tagaacaaga atttcgagca atttcaagcg
     3961 aagtggaatc cggttcgcgt gaagaaaatg cgctaaatca agatgctgga atcatttttg
     4021 gcgaattgca attcaccaga aagggttctt gcgatcaaat caaacgccgc tgtgcctttg
     4081 ccgcaatgat cgacgtcatc aaccttgcgc tgccgttctt cgggctcatt ctgctcgggt
     4141 ttttctgcgg ccgcttgatg aaaatgcccg aggccgggct ggcatggatg aatttctaca
     4201 tcgtctatgt cgcgttgccg cctctgttct tcaagctgat cgtcgcgacg ccgtttgccg
     4261 agttgcagaa ctggagcttt gtcgtcggca cgacgctttc gacctatctt gtgttcgttc
     4321 tgtcgctggc catcgggctg gcgttctacc ggggcgacct caagccggcg acgttgcagg
     4381 cggtgctcgg ggcctattcc aacatcggct acatggggcc ggggctgacg ctggccgcca
     4441 tcgggcccaa ggcagccgcg ccgacggcgc tgattttcgt gttcgacaac atcctgctgt
     4501 tttccctgat cccgttcctc atggcgctgg gtggaaccga gaagctggac gttgccagga
     4561 cggcgcgcat ggtgctgtgg aagatcgtct cgcatccgtt caacatcgcc gcgttcgtgg
     4621 cgctgatctt tgcctatttc cagctcaaac cgccgatggc gatcgaccgg atgctgacca
     4681 tgctctatgg gtcggcagct ccggtggcgc tgttcgcgct ggggctgacg gtggcctcgc
     4741 ggcgggtgac gcgcgtgccc gtcgaactgc ccatccacct ttgcgtcaag ctgttcctgc
     4801 acccggcgat catcctgctg ctgctgtcgc tgcttggccc cttcgagaag gaatgggtct
     4861 attctgccgt gctgatggcc tcgctgccgc cggcactcaa cgtgttcgtt gtggcgcggc
     4921 aatatgatgt ctacgtgcag gaagcctcca ctgcgatcct cctgggcacg ctggcctcga
     4981 tcgtcagcct caccttcgtg ctctacctga tcaccaccgg cgcgctgccg atcagcctgt
     5041 tcggctagag cattttcaag cgaagtggct ttccggttcg cgtcaagaaa atgcgaaaaa
     5101 ctgggaactc tagatcaagc cgcagcgata ggggatcgcc ccatgccggc ccgcatcagc
     5161 atccggcgga tcggtccgac atgggccgcg gcggtcagcc cggcaaagcg ggcaagatcg
     5221 agcggcagca acccggaaat gagcgagcgg ttgagcaggt cgacgccgta ggtgcgcgag
     5281 accgcgtcga tctgccggct acgctcatag ggcgccagag cggccgcact tcccacatcc
     5341 tcgccgcgct cgtgcgccga ccggatggcc gccaccaggc tcctggcatc gcggaagccg
     5401 aggttcatgc cctgcgcgcc cacaggcggg aacgcatgca gtgcctcacc ggcc
//
LOCUS       DAOMXG010000159         5451 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_7935_length_5451_cov_11.337658, whole genome shotgun sequence.
ACCESSION   DAOMXG010000159 DAOMXG010000000
VERSION     DAOMXG010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 5451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5451
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7935_length_5451_cov_11.337658"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1547)
                     /locus_tag="PLQ11_10145"
     CDS             complement(<1..1547)
                     /locus_tag="PLQ11_10145"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HRK25302.1"
                     /translation="MSSMSLAFGPVALVSDGGIAVIEIDNPPVNATSQAVRAGLLAAV
                     EAAEADATIHAVVIAAAGRTFVAGGDIAEFGKTPQLPHLPDVMNRIETLAKPVVVAWH
                     GTALGGGCEIGLAAHARVMAADASLGLPEVKLGLCPGAGGTQRLPRLVGIAKAIDIIG
                     SGRMVGAAEAAALGLTDMLADGDVRKAAIAKARAMIGKPVLRTSDRPTPPADEAAIAK
                     AVAAVTKDRHRLAPVAIADLVQLSARLPVAEGMPVERKAFFQLMESDQSKALRHVFFA
                     ERAVARVRGIEGVGARAVRSVGIVGAGTMGAGIAVAFLDAGYCVTIVETSVEALERGR
                     ARIGELYDRSLRFGRVSADGKAERLGRAIFSADLADLAGCDLVVEAVFEDMAVKKDLL
                     GKLETITRPDCILATNTSYLDIDDMASGLARPQNVVGLHFFSPAHVMKLLEIVRARKT
                     ASDVLATAVALGKRLRKVAVVCGVCDGFIGNRILARYRAQCEFMLEEGALPQEIDAAL
                     EAYGFAMG"
     gene            complement(1658..1948)
                     /locus_tag="PLQ11_10150"
     CDS             complement(1658..1948)
                     /locus_tag="PLQ11_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRK25303.1"
                     /translation="MLIAKAGKAEDFLKALANRHRLMILCELHKGERSVTALQEAVGL
                     SQSALSQHLARLRADSLVRTRREAQTIYYSLGSPEAKRMIALMYELFCNSEC"
     gene            2053..2400
                     /locus_tag="PLQ11_10155"
     CDS             2053..2400
                     /locus_tag="PLQ11_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRK25304.1"
                     /translation="MIDNWPEATRAFSANLRQLRGGAGETMKAFSALAQAALAPKALD
                     TKTKELIALAIGVAVRCDDCIAFHVKACIEQGASRDEILETLSMAIYMGAGPSVMYAS
                     HALAAYEQMAQKD"
     gene            2404..2742
                     /locus_tag="PLQ11_10160"
     CDS             2404..2742
                     /locus_tag="PLQ11_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011926153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRK25305.1"
                     /translation="MVFAALARALRGPGLSYDDVCKACQTGSHAIVDVREAGEFASGH
                     VPGAINMPLSRFDATRLPSDRPVILMCLSGGRSARALAACRGLGRNDILNYSGSYADW
                     CRNGGQTERG"
     gene            2827..4317
                     /gene="glpK"
                     /locus_tag="PLQ11_10165"
     CDS             2827..4317
                     /gene="glpK"
                     /locus_tag="PLQ11_10165"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012652016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HRK25306.1"
                     /translation="MGRFILAIDQGTTSSRAIVFRDDGSIAGVGQQEFAQHYPRSGWV
                     EHDPEDIWQTTLATIRSALAAARLTADQLAAIGITNQRETTIVWERQTGRPIHRAIVW
                     QDRRTAGACARLKRDGVEKLVAGRTGLLLDPYFSATKIAHILDTVPGARRRAERGELC
                     FGTVDSFLLWRLTGGASHATDATNASRTLLLNLHSGAWDDQLLTLFRVPRALLPEVKD
                     CAADFGVTDKAILGACVPILGIAGDQQAATIGQACFSPGMMKSTYGTGCFALMNTGTV
                     PVRSKKRLLTTIAYQLDGVRTYALEGSIFIAGAAVQWLRDGLGIIERASDAGPLAAKA
                     DASQNVVLVPAFVGLGAPWWDAEARGALFGLTRGTGPRELARAALESVCFQTVDLIEA
                     MRADWKGARESATVLRVDGGMTASDWTMQRLADLIGAPVDRPTIPETTALGAAYLAGY
                     RAGVFPTPDEFARRWQCEQRFAPAMSEADRRKRLRLWKAAVLRTLS"
     gene            4421..>5451
                     /locus_tag="PLQ11_10170"
     CDS             4421..>5451
                     /locus_tag="PLQ11_10170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HRK25307.1"
                     /translation="MKRLSSLISAGLQPLRNLAASISRRMSRLQIDAHRRGRFARTPF
                     AMPWLGWKDIVARTWEQIFNDRLLSVAAGVAFFALLALVPSLSVMLTVFALVADPAQI
                     EVQLAPLVGLMPDTAATLVMDQAHRLAQQAASQPMSLTLIVSLAIALWSANAGIQALF
                     DALNVIYDEDEKRSFLALNALSLAITLGAAVVLIVVVGLVTVLPVVVAKLPFAAAFEG
                     SVALLRWPTFLVLVTAALAVLFWIGPSRRRARFVWVLPGAALAALLWALVSALFNLYV
                     SRLGSYDVAYGSLSAVIVLMTWLWLSATAILIGAELNAELEHQTMRDTTEGPPKPIGA
                     RGARRADAIG"
BASE COUNT          923 a         1749 c         1753 g         1026 t
ORIGIN      
        1 cccatggcaa agccataggc ttcgagcgcg gcgtcgatct cctgcggcag ggcgccttcc
       61 tccagcatga attcgcattg cgcacggtag cgtgccagga tgcggttccc gataaagccg
      121 tcacagacgc cgcagaccac ggcaaccttg cgcaaccgct tgccgagcgc gacggcggtg
      181 gccagcacgt cagatgcagt ctttcgggcc cggacgattt ccagaagttt catgacatgg
      241 gccggcgaga agaaatgcag gccgaccaca ttctgcggcc gggcaaggcc ggatgccatg
      301 tcatcaatgt cgagatagct ggtattggtc gccaggatgc aatcgggccg ggtgatcgtc
      361 tccagtttgc ccagcaggtc cttcttgacg gccatatcct caaacaccgc ctcgaccacc
      421 aggtcacagc cggcaaggtc ggcaagatcg gcggaaaaga tggcacgccc gaggcgttcg
      481 gctttgccgt cggcgctcac ccgcccgaag cgcagcgagc ggtcatacag ctcaccgatc
      541 cgagcccggc cacgttccag cgcctccacc gacgtctcga cgatggtcac gcaataacca
      601 gcgtcgagga acgccacggc gattcctgcc cccatcgtgc cggcgccgac aatgccgacc
      661 gatcgcaccg ctcgtgcgcc gacgccttcg atccccctga cccgcgcgac cgcccgttcg
      721 gcaaagaaca catgccgcag cgccttcgat tggtccgatt ccataagctg gaaaaatgcc
      781 ttgcgttcaa ccggcatgcc ctcggcaacg gggagcctgg ccgatagctg caccagatcg
      841 gcgatggcga caggcgccag ccggtggcga tccttggtaa cagccgcaac ggcctttgct
      901 atggcagctt cgtcggccgg aggcgtcggc cgatcgctcg tccgcaggac aggctttccg
      961 atcatggcgc gcgccttggc aatcgcggcc ttgcgcacat cgccatcggc gagcatatcg
     1021 gtaaggccga gcgcggccgc ttccgctgct ccaaccatgc gacccgagcc gatgatgtcg
     1081 atggccttgg cgatcccgac cagacggggc agccgctgcg taccacccgc gcccgggcac
     1141 agcccgagct tgacctcggg caggccaagc gatgcatcgg cggccatcac ccttgcatga
     1201 gccgcaaggc ctatctcgca cccgccgccc agtgccgttc catgccaggc cacgaccaca
     1261 ggttttgcga gggtctcgat gcggttcatc acatcgggca gatgtggcaa ttgcggggtc
     1321 ttgccgaatt cggcgatatc accgccggca acaaaggtcc gtccggccgc cgcgatgacc
     1381 acagcgtgga tggttgcatc agcctcggct gcctcaacgg cagccagcag tccggcccgc
     1441 acggcctggg aggtggcatt gaccggcggg ttgtcgattt cgatgaccgc gatccctcca
     1501 tcactgacca gcgcgacagg accaaaggca agcgacattg aggacatggg tgtttccgga
     1561 ctgaggcatt gtccggacag tttgcccagc ttggcgcgcc acacaagagc gctatggcgg
     1621 ttttacgcaa aaagactgac cgagcggtca gctataatca gcactcactg ttgcagaaca
     1681 gctcatacat cagcgcgatc atccgcttgg cctccgggct gcccagagaa tagtagatgg
     1741 tctgggcttc gcgccgtgtc cgcaccagcg aatcagcacg cagccgcgcc agatgctgcg
     1801 acagcgccga ttggctgagc ccgaccgcct cctgcagcgc ggtgaccgat cgctctccct
     1861 tgtgcaattc acacaggatc atcaggcggt ggcggtttgc cagcgctttg agaaagtcct
     1921 cggccttgcc ggccttggct atcagcattt cgacttgtgc atcatacata ttagggctat
     1981 ctaatataat ggcagctgcg gacgtttggc aggtctgcta ctggctccat accacgatcc
     2041 ttagaggaaa acatgatcga caattggcct gaagccacca gagcattttc ggcaaacctg
     2101 cgccagctcc gcggcggggc aggcgagacc atgaaggcct tttcggcgct tgcgcaggca
     2161 gcgctggctc ccaaggcgct tgataccaag accaaggaac tgatcgcgct ggcgatcggc
     2221 gtggcggtgc gctgtgatga ttgcattgcc tttcacgtca aggcctgcat cgagcaggga
     2281 gccagccgcg acgagattct cgagacgttg tcgatggcta tctacatggg agccggtccg
     2341 tcggtgatgt atgccagcca cgccctggcg gcctacgagc agatggcaca gaaggactag
     2401 accatggtgt ttgccgctct ggcgcgggcg ctcaggggcc cgggcctttc ctatgatgac
     2461 gtctgcaagg cttgtcagac aggttcgcat gcgatcgttg atgtgcgcga agctggggaa
     2521 tttgcttccg ggcatgtgcc gggagccatc aacatgccac tgtcgcgttt cgatgccacc
     2581 cggctgccct cggaccggcc tgtcatcctg atgtgcctgt cgggaggccg ttcagcccgt
     2641 gcgcttgcag cgtgccgggg gctgggtcgc aacgatatcc tcaactattc cggcagttac
     2701 gccgattggt gccgcaacgg cggccagact gagcgaggct gatccactac ccttgtcttg
     2761 cctggcaaga tcgactgtta tctcggcacg gggcaggaat gagcacgtcc ccgggcagga
     2821 ggcagcatgg gacggtttat cctcgccatc gatcagggca ccacatcaag ccgtgccatc
     2881 gtgttccgcg acgatggatc gatcgccggg gttgggcagc aggagtttgc ccagcactat
     2941 ccgaggagcg gctgggtcga gcatgaccct gaggacatct ggcagacaac cctcgcaacc
     3001 atccgcagcg ctctcgccgc cgcccggctc accgccgatc aactcgctgc catcggcatc
     3061 accaaccagc gcgagacgac aatcgtctgg gaacggcaga ccggccgtcc gatccatcgc
     3121 gccatcgtct ggcaggaccg acgcacggcc ggagcctgcg cacggctgaa gcgtgatggt
     3181 gtggaaaagc tggtggcagg ccggacgggt cttctgctcg atccctattt ttccgccacc
     3241 aagatcgccc atatcctcga tacggtgccg ggcgcgcggc gcagggccga gaggggcgaa
     3301 ctgtgctttg gcaccgtcga cagtttcctg ctctggcggc tgaccggtgg cgcgtctcat
     3361 gccaccgatg ccacaaatgc ttctagaacc ttgctgctca accttcattc cggcgcctgg
     3421 gacgaccagt tgctgaccct gttccgggtg ccgcgcgccc tgttgccgga ggtcaaggat
     3481 tgcgctgccg atttcggcgt aaccgacaag gcgatcctgg gcgcttgtgt tccgatcctc
     3541 gggattgccg gtgaccagca ggccgccacc atcggccagg cctgtttcag ccccggcatg
     3601 atgaaatcga cctatggcac cggctgcttc gctctgatga ataccggcac ggttcccgtg
     3661 cgctcgaaaa agcggctcct gaccaccatc gcctaccagt tggacggggt gcgcacctac
     3721 gcgctcgaag gctcgatctt catcgccggc gcggccgtgc aatggctgcg cgatggtctc
     3781 ggcatcatag agcgggcgtc cgacgccggg cctctggcgg ccaaggcgga tgccagccag
     3841 aatgtcgttc tggtccccgc ctttgttggt ctcggggcgc cctggtggga tgccgaggcg
     3901 cgcggcgcat tgttcggcct gacgcgtggc accggcccgc gggaactggc ccgcgctgcg
     3961 ctggaaagtg tctgtttcca gacggtcgat ctgatcgagg cgatgcgggc tgactggaag
     4021 ggagcccgcg agtcggccac tgtcctgcgc gttgatggcg gtatgaccgc cagcgactgg
     4081 accatgcagc gccttgccga tctcatcggc gcaccggtgg accggccgac aataccggaa
     4141 accacggcgc tgggggccgc ctatctggcc ggctaccggg ccggggtgtt tcctactccg
     4201 gatgagttcg cacggcgctg gcaatgcgag cagcgctttg ccccggccat gtcggaggcg
     4261 gatcggcgca agcggctcag gctgtggaag gcagcggtct tgcgcacatt gagctgaagc
     4321 ggtcagccct gaacgccgcc gctgggatcg aacggatggt cgggtccgtg gtagttggag
     4381 gcagtcccct ccgttgattc gggagtgtcg agcggccccc atgaaacgcc tgtcgtccct
     4441 gatctccgcc gggctccagc cgttgcgcaa tctcgcggcc tcgatcagcc ggcggatgtc
     4501 gcgcctgcag atcgacgcgc atcggcgcgg ccggttcgcc cgcacgccct tcgccatgcc
     4561 gtggctgggc tggaaggaca tcgtcgcgcg cacctgggag cagatcttca acgatcgcct
     4621 gctgtcggtt gctgcgggcg tggcgttttt tgctctgctg gcgctggtgc cgagcctgtc
     4681 ggtgatgctg acggttttcg cgctggtggc cgatccggca cagatcgagg ttcaactggc
     4741 cccgctcgtt gggctgatgc ccgacacggc ggcgacgctt gtcatggatc aggcccaccg
     4801 gttggcgcag caggcggcca gccagccgat gtcgctgacc ctgatcgtca gcctggcgat
     4861 cgccctgtgg agcgccaacg ccggaatcca ggcgttgttc gacgcactga acgtcatcta
     4921 cgacgaagat gaaaagcgtt cgtttcttgc tctgaacgcc ttgtcgcttg cgatcacgct
     4981 gggggctgcc gtcgtactga tcgtcgttgt cggcctggtc acggtgctgc cggtcgtggt
     5041 cgcaaagctg ccgtttgcgg cggcgttcga ggggagtgtg gcgctgctgc gctggccgac
     5101 attcctggtg ttggtcactg cggcgctggc tgtcctgttc tggatcggcc cgagccggcg
     5161 gcgtgccaga ttcgtttggg tattgcccgg cgcggcgctt gcggccctgc tctgggcgtt
     5221 ggtctccgcg ctgttcaatc tctatgtcag ccggcttgga agctacgatg tcgcctatgg
     5281 atcgctttcg gcggtgattg tgctgatgac ctggttgtgg ctttcggcca ccgccatcct
     5341 gatcggtgcc gagttgaatg ccgaactcga gcatcagacc atgcgggata cgaccgaggg
     5401 accgcccaag cccatcggag cgcggggagc ccggcgcgcc gatgcgatcg g
//
LOCUS       DAOMXG010000160         5432 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_7971_length_5432_cov_7.867026, whole genome shotgun sequence.
ACCESSION   DAOMXG010000160 DAOMXG010000000
VERSION     DAOMXG010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 5432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5432
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7971_length_5432_cov_7.867026"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..147)
                     /locus_tag="PLQ11_10175"
     CDS             complement(<1..147)
                     /locus_tag="PLQ11_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611034.1"
                     /note="Catalyzes the reversible hydration of unsaturated
                     fatty acyl-CoA to beta-hydroxyacyl-CoA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HRK25308.1"
                     /translation="MSVQTPILTRSDDGGIATLTLNRPEARNALSEAMIAALAAEMAA
                     IADDR"
     gene            205..675
                     /locus_tag="PLQ11_10180"
     CDS             205..675
                     /locus_tag="PLQ11_10180"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRK25309.1"
                     /translation="MPSHKPALSREEMIAFLEAVFPEVMGQGANMQVEDVGYGTCRIR
                     LKYKSRQLRPGGTISGPTMMALADFSVYVAVLSALGKVALAVTTNLNINFLKKPSPGD
                     LIADCRLFKIGKRLCVGEAMIHGEADDDLVAHAVATYSVPPEREGGDRGLSPPV"
     gene            1106..1570
                     /gene="rplM"
                     /locus_tag="PLQ11_10185"
     CDS             1106..1570
                     /gene="rplM"
                     /locus_tag="PLQ11_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012170717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HRK25310.1"
                     /translation="MKTYSAKTAEVEKKWLLIDAKGLVVGRLASIIAMRLRGKHKPTF
                     TPHIDCGDNVIVINAEKVVLTGRKRDNKVYYHHTGYIGGIKERTAKFILEGRYPERVV
                     EKAVERMLPRGPLGRQQLSNLRVYKGDKHPHEAQQPVALDVASLNSKNSRLG"
     gene            1570..2046
                     /gene="rpsI"
                     /locus_tag="PLQ11_10190"
     CDS             1570..2046
                     /gene="rpsI"
                     /locus_tag="PLQ11_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HRK25311.1"
                     /translation="MATLQSLGDLAGTAKPAAPEAPVHVQKLDKQGRAYATGKRKDAV
                     ARVWIKPGKGKITVNDRDVEVYFARPVLRMILQQPFGVAGRVGQYDVMVNVVGGGLSG
                     QAGAVRHGLAKALTYYEPELRSPLKKAGFLTRDSRVVERKKYGHKKARRSFQFSKR"
     gene            complement(2106..2390)
                     /locus_tag="PLQ11_10195"
     CDS             complement(2106..2390)
                     /locus_tag="PLQ11_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25312.1"
                     /translation="MIRFGKGFAAALATALILGMANTALATGPGGGIRPLSGAEAEAA
                     RGNTIAQDRCMSICQQSARTPAGQAQCPNNCTAGRCYHNPASGRSYCIAR"
     gene            2525..3481
                     /gene="speB"
                     /locus_tag="PLQ11_10200"
     CDS             2525..3481
                     /gene="speB"
                     /locus_tag="PLQ11_10200"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009759945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HRK25313.1"
                     /translation="MTDKHPPVDNAFTGPLLGGAEEATFSGALSFMRRRYSKDAASAD
                     VVVWGVPFDTATSNRPGARFGPQAIRRASAIFDGDPQYPSRRDPFQRLAAIDYGDCVL
                     PRGDQSLAPDAIARQAGKILASGAHLMTMGGDHFITLPLLRAHAARHGPLALVQFDAH
                     QDTWDDGPGAISHGSFVTEAVREGLIDPDRSVQVGIRTIAPRDHGITIIDAYEAQSLG
                     VGGLVEEIRSAIGDGPAYLSFDIDCLDPAFAPGTGTPVAGGLSSAMALRTIWALRDCD
                     WRGMDVVEVSPPYDHADVTAIAAAALMNHYIQGLAERVSRSR"
     gene            3815..4750
                     /gene="argC"
                     /locus_tag="PLQ11_10205"
     CDS             3815..4750
                     /gene="argC"
                     /locus_tag="PLQ11_10205"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HRK25314.1"
                     /translation="MSAKQHTVFIDGEAGTTGLGIRERLAGLPGIAMRSIAPEKRKDP
                     EAKKALMAEVDVVILCLPDDAAKETAAIVAGMGAKGPRLLDASTAHRVAPGWVYGFAE
                     LAPGQDRKIARARNVANPGCYPTGAIALLRPLIDAGVMPRTYPVTVNAVSGYSGGGKS
                     MIEAYEAGRAPAFELYGLGLEHKHVPELMAYTGLSRRPIFVPSVGNFRQGMLVSVPLH
                     LDTLEGKPRAADLEAILRAHYAGSEWVSVATGEDAALNAGKIDALALNDTNKMELFVF
                     ANQARGHAVLVARLDNLGKGASGAAVQNLQLMLGL"
     gene            complement(4871..>5432)
                     /locus_tag="PLQ11_10210"
     CDS             complement(4871..>5432)
                     /locus_tag="PLQ11_10210"
                     /inference="COORDINATES: protein motif:HMM:NF025703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDYXXLXY domain-containing protein"
                     /protein_id="HRK25315.1"
                     /translation="GAVSALAPVAPLGITPGAEASGRFDRALITGGVLATIAIATLGI
                     RDKEAIISGGRILHIALAPVDPRSLIQGDYMALRFALPNDPAGLKLINDASKHPIALA
                     RTDNRQVATVTRIAPEGTQPGEGEFPLILTKRKGRWSLGTDAWYFAEGSAKKFEVAKF
                     GTFRVSQKGDMILVGMADEHLRPIQP"
BASE COUNT          911 a         1763 c         1748 g         1010 t
ORIGIN      
        1 ccggtcgtcg gcgatcgccg ccatttcggc ggccagcgcc gcgatcatcg cctcggagag
       61 cgcgttgcgc gcctccggcc ggttgagcgt gagggtggcg atgccgccgt catcgctgcg
      121 agtgaggatt ggggtctgga cgctcatggt gggaccggtt acctctcaac cattgccatg
      181 caggaaccag tgtagatgtc cgccatgccc tcccacaagc ccgcgctcag ccgcgaggag
      241 atgattgcct ttctcgaagc ggtgttcccc gaggtgatgg ggcagggcgc caacatgcag
      301 gtcgaggatg tcggctatgg cacctgccgc atccggctga aatacaagtc gcggcagttg
      361 cgccccggcg gcaccatctc cgggccgacg atgatggcgc tggccgattt ctcggtctat
      421 gtcgcggtgc tttcggcgct cggcaaggtg gcgctggcgg tgacgaccaa cctgaacatc
      481 aatttcctca agaagccatc gcccggcgat ctgatcgccg attgccggct gttcaagatc
      541 ggcaagcgcc tgtgcgtcgg cgaggcgatg atccacggcg aagccgatga cgacctcgtc
      601 gcccatgcgg tggcgaccta ttcggtgccg cccgagcgcg agggcggcga tcgtggcctg
      661 agcccgccgg tgtgaggggc catcacaggt cgagcgcgcc ttgcgagcct gtgttctccc
      721 gcttggcctt gcggcgcatg gttttgggca agcgggggag gcccccaccc ggtcctccgc
      781 ccgcagacaa gtctacgcag actgcgtaaa cttgcccgcg cgctcggacc accctcccca
      841 cgagggggag ggatttcaac gggcgccgcg tccattccct cccccttgat gggagggtcg
      901 gcgcgtcagc gccggggagg ggtgaagcat gaggtaaaat aataccgcaa ttgctaactc
      961 attcaaaaac aatgataatt caaatatgtt ggtcatgcgc gcccccttga cgctgcaaga
     1021 tcaatcgctt agagacgggg caacctcggc ggcgggcttt ccgccgcctt tgtgttgaat
     1081 ccatcaaccc cccggggatt attccatgaa gacctattcg gccaagaccg ccgaggtcga
     1141 gaagaagtgg ctgctgatcg acgcgaaagg tctcgtcgtc ggccgcctcg cctcgatcat
     1201 cgcgatgcgt ctcagaggca agcacaagcc tacgttcacg ccccacatcg actgcggcga
     1261 caacgtcatc gtcatcaacg ccgagaaggt ggtactcacc ggccgcaagc gcgacaacaa
     1321 ggtgtattac catcacaccg gctatatcgg cggcatcaag gagcgcacgg ccaagttcat
     1381 tcttgagggc cgctatcccg agcgtgtcgt cgagaaggcg gtggagcgca tgctgccgcg
     1441 cggcccgctt ggccgccagc agctgtcgaa cctgcgcgtc tacaagggcg acaagcaccc
     1501 gcatgaggcc cagcagccgg tggcgctcga cgtcgcttcc ctcaactcca agaattcgag
     1561 gctcggctaa tggctacgct ccagtccctc ggtgatctgg ccggcaccgc caagccggcc
     1621 gctccggaag ctccggttca cgttcagaag ctcgacaagc agggccgcgc ctatgccacc
     1681 ggcaagcgca aggatgcggt cgcccgcgtc tggatcaagc ccggcaaggg caagatcacg
     1741 gtcaacgatc gcgatgtcga ggtctatttc gcccgccccg tgctgcgcat gatcctgcag
     1801 caaccgtttg gtgtcgcggg tcgcgtcggc cagtatgacg tgatggtcaa cgtggtcggc
     1861 ggtggcctgt ccggccaggc cggcgcggtg cgtcacggcc ttgccaaggc gctgacctat
     1921 tacgagccgg agctgcgctc cccgctcaag aaggccggct tcctcacccg cgacagccgc
     1981 gtggtcgagc gcaagaagta tggccacaag aaggcccgtc gcagcttcca gttctcgaag
     2041 cgttaagcgg aaccgcgata cattgcagaa cagggcgggc gtcagtccgc cctttttgtt
     2101 ttggcttacc gcgcgatgca gtaggaccgg ccggatgccg gattgtggta gcatcgcccg
     2161 gccgtgcaat tgttcgggca ttgcgcctgt ccggcaggag tgcgcgcgct ttgctggcag
     2221 atcgacatgc agcggtcctg ggcaatggta ttgccgcgcg ccgcttcggc ctctgcgccg
     2281 ctaagcggcc ggatgccgcc gcccggcccg gtggccagcg cggtgttagc catgcccaga
     2341 atgagtgcgg tcgccagcgc cgccgcaaag cctttgccaa acctgatcat gtgttccccc
     2401 gctacggatc atggacacgt ccgattctgt atcaggcacc ggacatgtca atcggcgccg
     2461 caaagttgtt gatgcgcggc ccgccgccct tgcggtatgg catggccggt tgttgacttc
     2521 ctgcatgacc gacaagcacc cgcccgtcga caatgccttt accggcccgc tgctcggcgg
     2581 ggcggaggag gcgacctttt ccggcgccct gtcgttcatg cgccggcgct attccaagga
     2641 tgccgcgagc gccgatgtgg tggtctgggg cgtcccgttc gacaccgcga cctccaaccg
     2701 gcccggcgcc cgcttcggtc cccaggcgat ccgccgggcc tcggcgatct ttgacggcga
     2761 tccgcaatat ccctcgcggc gcgatccgtt ccagcgcctc gcggcgatcg actacggcga
     2821 ttgcgtcctg ccgcgcggcg accagagcct ggcccccgat gccattgccc ggcaggccgg
     2881 caagatcctc gcctcgggcg cgcatctgat gaccatgggc ggcgatcatt tcatcactct
     2941 gccgctgctg cgcgcccatg ccgcgcggca tggaccgctg gcgctggtgc agttcgacgc
     3001 gcatcaggat acctgggacg acgggccggg cgccatcagc catggcagct ttgtcaccga
     3061 ggcggtgcgc gaggggctga tcgatccgga tcgctcggtg caggtcggca tccgcacaat
     3121 cgcgccgcgc gatcatggca tcaccatcat cgacgcctat gaagcccagt cgctgggagt
     3181 gggcgggctg gtcgaggaaa ttcgctccgc gatcggtgat gggccggcct atctcagttt
     3241 cgatatcgac tgtcttgacc ctgcctttgc gccgggcact ggcactcccg tggccggcgg
     3301 gctgagttcg gcgatggcgc tgcgcacaat ctgggccctc agggattgcg actggcgcgg
     3361 catggacgtg gtggaggttt ccccgcccta tgatcatgcc gatgtcacgg cgattgccgc
     3421 tgcggcgctg atgaatcatt acattcaggg cctcgcggag agggtcagcc gcagccgcta
     3481 accgattcag atgattcgcc aaatcatccg aagttgaatc actttttgag cagatggatt
     3541 cttgcagtga acccgcgtta ttgacgcttt gagcatttgg cttgccactg cgtccggctg
     3601 ccggcacagt tgcgcccaag ccccttaagc cattgacaga gcgggcattc atccgcatac
     3661 ggaaaggcat gaacaccatg aaccgatacc gcacggtctt gagcgctgtc ctgcgcctgc
     3721 gatgagccgt gccctccgtg ccgaagctcc gcagccgcgt gggaaacgcg gcttttttat
     3781 tgccctgatc tgaccgaggg aaaggatccc tgccatgagc gccaaacagc atacggtttt
     3841 catcgacggt gaggctggca ccaccgggct tggaatccgc gagcggctgg ccggcttgcc
     3901 cggcattgcc atgcgctcca tcgcgccaga gaagcgcaag gatccggagg ccaagaaggc
     3961 gctgatggcc gaggtggatg tggtcatcct ctgcctgcct gacgatgcgg cgaaagagac
     4021 ggcggccatc gtcgccggca tgggcgcgaa ggggccgcgc ctgctcgatg cctccaccgc
     4081 ccaccgcgtc gcccctggct gggtctatgg ttttgccgag cttgcgcccg gccaggaccg
     4141 caagatcgcc cgggcgcgca acgtcgccaa tcccggctgc tatcccaccg gagcgatcgc
     4201 gctgctgcgg ccgctgatcg atgccggtgt gatgcccagg acctatcctg tcaccgtcaa
     4261 tgccgtgtcc ggctattcgg gcggcggcaa gtcgatgatc gaggcctatg aggcgggccg
     4321 tgcgccggct ttcgaattgt atggccttgg tcttgagcac aagcatgtgc cggagctgat
     4381 ggcctatacc gggttgagcc ggcggccgat cttcgtgccc tcggttggca atttccgcca
     4441 ggggatgctg gtctcggtgc cgctgcatct cgacacgctg gagggcaagc cgcgggcagc
     4501 cgacctcgaa gccatcctca gggctcatta cgccggctct gaatgggtaa gcgtcgccac
     4561 cggcgaggat gcggcgctga atgccggcaa gatcgacgct ctggcgctca acgacaccaa
     4621 caaaatggag cttttcgttt ttgccaatca ggcgcgcggc catgctgttc tggtcgcccg
     4681 gcttgacaat ctcggcaagg gagcctccgg cgcggccgtg cagaatttgc agctgatgct
     4741 gggactttga tcaaacacca ccccaccccg ctcgtttcac tcgcggccct cccctgtgag
     4801 gggagggagg gcggtccaat cttccatccc cgttccgggg aggtgtcgcg tcagcgaccg
     4861 ggtgggggtg ctacggctgg atcggtcgca aatgctcgtc ggccattcct accaggatca
     4921 tgtctccttt ttgagagacc cggaacgtgc cgaatttcgc cacttcgaac ttcttggccg
     4981 agccttcggc aaaataccag gcatcggtgc caaggctcca gcgtcctttc cgtttggtca
     5041 ggatcagcgg gaactcgcct tcgccaggct gtgttccctc cggtgcgatg cgcgtaacgg
     5101 ttgccacctg gcgattgtcg gttcttgcca gcgcaatcgg atgtttgctg gcgtcgttga
     5161 tcagcttgag gccggcagga tcattcggca gggcaaagcg cagggccata tagtcgccct
     5221 ggatcagcga gcgcggatcg accggcgcca gcgcgatatg caggatacgc ccgccgctga
     5281 tgatcgcctc cttgtcgcga atgcccagcg tggcgatggc gatcgtcgcc agcacgccgc
     5341 cggtgatcag cgcgcggtcg aaccgtccgc tggcttcggc acccggcgtg atgccgagcg
     5401 gcgccaccgg cgcaagcgcc gagaccgccc cc
//
LOCUS       DAOMXG010000161         5394 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_8053_length_5394_cov_10.777674, whole genome shotgun sequence.
ACCESSION   DAOMXG010000161 DAOMXG010000000
VERSION     DAOMXG010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 5394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5394
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8053_length_5394_cov_10.777674"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..1692)
                     /locus_tag="PLQ11_10215"
     CDS             complement(91..1692)
                     /locus_tag="PLQ11_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRK25316.1"
                     /translation="MTEAPLVSVRDLSVAFRQGGKETIAVDHVSFDIRRGETLALVGE
                     SGSGKSVSALSIVRLLAYPAASHPSGEILLAGEDMLKVDEARLRQIRGNVVTMIFQEP
                     MTSLNPLHTVERQISEILSIHQGLSGEAARARTLELLTRVGIPDAESRLGAYPHQMSG
                     GQRQRVMIAMALANKPDLLIADEPTTALDVTVQAQILELMRDLQRETGMAMLFITHDL
                     GVVRRIAHRVAVMRKGRIVETGPVAEVFSNPQHEYTRALMAAEPKGLPPPEDATKPVI
                     LSADGLKVWFPIRKGLLRRVVGHVKAVDGISFAVRAGQTVGVVGESGSGKTTLGLAAL
                     RLIRSDGPIVFLGRRIDTLGYSALRPLRENLQIVFQDPYGSLSPRLSVAEIVEEGMLI
                     QNRSLSYEDRRARVAAALDEVGLDPAAMDRYPHEFSGGQRQRIAIARAMVLNPDFVVL
                     DEPTSALDMSVQAQIVALLRKLQAERNLAYLFISHDLKVVRALANRLIVMRHGKVIEE
                     GPAAEVFANPRDDYTRTLLSAAFER"
     gene            complement(1689..2834)
                     /locus_tag="PLQ11_10220"
     CDS             complement(1689..2834)
                     /locus_tag="PLQ11_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007066276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRK25317.1"
                     /translation="MLAADDRSPVAATAPRSRWALSPINRRRLDNFKRNRRGLWALWL
                     FLGLFVTSLFAEFIANDRPIIASYKGEVLFPVFVDYPEEKFGGFLARTDYRDPVIAGE
                     ITANGWMVWPPIRFSYETINRDLPAPAPSAPTWSLRDAQCQAVAVKTGGSSCRYLEWN
                     WLGTDDRGRDVVARLIYGFRISVLFGLALALVASLVGIAAGAVQGYFGGWIDLVMQRF
                     IEIWTSIPRLYLLITISSVVAPGFFVLLGILLLFEWTALVGVVRAEFLRARNFEYVNA
                     ARALGLADATIMVKHVLPNAMVATLTLLPFVLNGSITTLTSLDFLGFGLPPGSPSLGE
                     LLQQGKSNLQAPWLGVSGFFVIALMLSLLNFIGEAVRDAFDPRKTFA"
     gene            complement(2848..3948)
                     /locus_tag="PLQ11_10225"
     CDS             complement(2848..3948)
                     /locus_tag="PLQ11_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HRK25318.1"
                     /translation="MLAYILRRLLLMIPTLLGILLVAFVIVQFAPGGPVERVLAQLQG
                     ADTSASSRVGGGTGDLGGQAALQSAQASSTYRGAQGLDPAFIKELEKQFGFDKPAHER
                     FFKMLWDYMRFDFGKSYFRDATVLELIKEKLPVSMSLGLWMLLISYGISIPLGIRKAV
                     KDGTPFDVWTSALIVTGYAMPGFLVAVFLIVLFAGGSFFNLFPLRGLTSENWESLSLA
                     GKVLDYLWHITLPIVAMALSAFATLTFLTKNSFLDEIRKQYVLTARMKGLGERSVLYG
                     HVFRNAMLIVISGFPSAFIHAFFSGSILIETIFSLDGLGLLSFESILNRDYPVVFANL
                     YIFALVGLLINLITDLTYTLVDPRIDFEAREV"
     gene            complement(3972..4499)
                     /locus_tag="PLQ11_10230"
     CDS             complement(3972..4499)
                     /locus_tag="PLQ11_10230"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRK25319.1"
                     /translation="MPEAGLPVERGYRPGLIGACIALHGLSYAALSGFGASFEARIAE
                     SLSEFAGRMQRPVNGLWSVHRGDTLLGTIAIDGEDLGPGKAHLRWFVVAAEARGSGIG
                     RRLLEAALGHCDASGFAAVHLWTFAGLDAACHLYETHGFALVDEKPGAQWGVAVIEQE
                     YRRPHPRQGSVNPIS"
     gene            complement(4523..>5394)
                     /locus_tag="PLQ11_10235"
     CDS             complement(4523..>5394)
                     /locus_tag="PLQ11_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRK25320.1"
                     /translation="HNTRRPQFKDRRLREAVGLAFDFEWANKNLMYDAYRRTVSYFEN
                     STLKASGPPSSEELKLLEPFRGKVPEEVFGEPYVPPVSDGSGQDRALLRKAQQLLTEA
                     GCKRDPKGILLLPGGQPFTIEFMEDEPSLNRHLQGFVKNLKLLGIEATIRVVDATQFQ
                     RRVEDFDFDLISRRFSMSETPGESLKSFFHSEHANTRASRNMAGIADPVVDALVQSAI
                     DAKSRVDLTHAVRALDRVLRAGRYWVSAWYSGKHRVAYWDVFDRPKIDTRYGYNPVGL
                     WWFNAEKAKKIGL"
BASE COUNT         1108 a         1698 c         1703 g          885 t
ORIGIN      
        1 tggagcccgg ttcgcgcaaa gaaaatgcga caaaacaaaa agatagagca cttttggtga
       61 attggaattc actgaaagcg ctctagccaa tcaccgttcg aaggcggccg agagcagggt
      121 gcgcgtgtag tcatcgcgcg gattggcaaa cacctcggct gccggccctt cctcgatcac
      181 tttgccatgg cgcatcacga tcagccggtt ggcgagcgcg cgcaccacct tgagatcgtg
      241 gctgatgaac agataggcca ggttccgctc ggcctgcagc ttgcgcagca gggcgacaat
      301 ctgcgcctgc accgacatgt cgagcgccga ggtcggctcg tccagtacga cgaaatcggg
      361 attgaggacc atcgcccggg cgatcgcgat gcgctggcgc tgtccgcccg agaattcgtg
      421 cgggtagcgg tccatggcgg caggatcgag cccaacctcg tcgagcgcgg cagccacgcg
      481 cgccctccgg tcttcatagc tgaggctgcg attctggatc agcatgccct cttcgacgat
      541 ctcggccacc gacaggcgcg ggctgagcga tccgtaggga tcctgaaaca cgatctgcag
      601 gttctcccgc aatggtctga gagccgaata gcccagcgta tcgatccgcc tgccgaggaa
      661 gacgatcggc ccgtcagagc ggatcagccg cagcgcggcc agtcccagcg tcgtcttgcc
      721 cgatcccgat tcgccgacaa cgccgacggt ctgcccggcc cggaccgcga aggaaatgcc
      781 atcgaccgcc ttcacatggc cgacaacgcg gcgcaacaat cccttgcgga taggaaacca
      841 aaccttgagg ccatcggccg aaaggatgac cggtttggtg gcatcttccg gcggaggcag
      901 ccccttgggc tcggccgcca tcagggcgcg cgtatactcg tgctgcgggt tggaaaacac
      961 ctcggcgacc ggcccggtct cgacaatccg acccttgcgc atcacggcaa cgcgatgggc
     1021 aatgcggcgc accacgccga gatcatgggt gatgaacagc atcgccatgc cggtttcacg
     1081 ctgcagatcg cgcatcaatt cgaggatctg cgcctgcacg gtcacgtcga gcgctgtggt
     1141 cggttcgtcg gcgatcaaca gatccggctt gttggccagc gccatggcga tcatcacgcg
     1201 ttggcgctgc ccgcccgaca tctggtgcgg ataggcgcca agccggcttt cggcatcggg
     1261 aatgccgaca cgggtcaaca gctccagcgt gcgcgcccgc gccgcttcgc cgctcaatcc
     1321 ctgatggatc gacaggatct cgctgatctg ccgctcgacc gtatggagcg ggttgagcga
     1381 ggtcatcggc tcctggaaga tcatggtgac gacattgccg cggatctgcc tgagccgcgc
     1441 ctcgtcgacc ttcagcatat cctcgccagc cagcaggatt tcgccggagg gatgcgaggc
     1501 ggcaggatag gccagcagcc gcacgatcga cagcgccgag accgacttgc ccgaaccgga
     1561 ttcgccgacc agcgccagtg tctcgccgcg ccggatgtcg aagctgacat gatcgacggc
     1621 gatggtctcc ttccccccct ggcggaaggc gaccgacaga tcgcgcacgg agacgagcgg
     1681 agcctccgtc atgcgaaggt ctttctggga tcgaatgcgt cgcgcaccgc ctcgccgatg
     1741 aaattgagca gggacagcat cagcgcgatg acgaagaagc cggaaacgcc cagccagggc
     1801 gcctgaaggt tggacttgcc ctgttgcaag agttctccca gcgagggcga gcccggcggc
     1861 aggccgaagc cgagaaagtc gaggcttgtc agggtggtga tcgagccgtt gagcacgaag
     1921 ggaagcaggg tcagagtcgc caccatcgca ttgggcagca catgcttgac catgatggtg
     1981 gcgtcggcaa gccccagggc gcgcgcggca ttgacgtatt caaaattgcg cgccctgagg
     2041 aattcggccc gcaccacgcc caccagcgcc gtccattcga acagcagcag gatgccgagc
     2101 aggacgaaaa agcccggtgc caccaccgac gagatggtga tcagcaggta aagccgcgga
     2161 atcgaggtcc agatctcgat gaagcgttgc atgacaagat cgatccaacc gccgaaatag
     2221 ccctgcaccg ccccggcggc gatcccgacc agcgaggcaa ccagtgccag cgccagcccg
     2281 aacagcaccg aaatgcggaa gccgtagatc agccgcgcca ccacatcgcg gccgcgatca
     2341 tcggtcccga gccagttcca ttccagatag cggcaggaac tgccgccggt cttgacggcg
     2401 acagcctggc attgcgcgtc tctgagcgac caggttggcg cagaaggcgc cggcgccggc
     2461 aggtcgcggt tgatcgtctc gtatgagaag cggatcggcg gccacaccat ccagccattg
     2521 gccgtgatct cgccggcgat caccgggtca cgataatcgg tgcgggcgag aaagccgccg
     2581 aatttctctt ccggatagtc gacgaacacc ggaaacagca cttctccctt gtaggaagcg
     2641 atgattggcc ggtcattggc gatgaattcg gcaaacagcg aggtgacaaa cagtccgagg
     2701 aacagccaga gtgcccagag gccgcgccgg ttgcgcttga aattgtccag ccgccggcgg
     2761 ttgatcggcg acagcgccca gcgcgaccgc ggggccgtgg ccgcaacggg tgaacgatca
     2821 tcggccgcaa ggatcgagcc ggtcacatca cacctcccgc gcctcgaagt cgatccttgg
     2881 gtcaaccagc gtataggtaa gatccgtgat caggttgatg agcaggccga cgagcgcgaa
     2941 aatgtagaga ttggcaaaga ccaccggata gtcgcggttg aggatcgatt cgaacgacag
     3001 cagccccagc ccgtcgagcg aaaagatcgt ctcgatcagg atcgagcctg aaaagaacgc
     3061 gtggatgaag gcgctcggaa agccggaaat gacgatcagc atggcattgc ggaacacatg
     3121 gccgtagagc acggagcgct cgcccagtcc cttcatccgc gccgtcagca catattgctt
     3181 gcggatttcg tcgagaaacg agttcttggt caggaaagtg agggtcgcaa aggcgctgag
     3241 cgccatggcc acgatcggca gcgtgatgtg ccagaggtaa tcgagcacct ttccggcaag
     3301 gctgaggctc tcccagttct cgctggtcag gccacgcagt ggaaacaggt tgaagaacga
     3361 gccgccggca aacaggacga tcaggaacac cgcgaccaga aagcccggca tggcatagcc
     3421 ggtgacgatc agcgccgagg tccagacatc gaaaggcgtg ccgtccttga ccgccttgcg
     3481 gatgccgagc gggatggaga tgccatagga aatcagcagc atccacagcc ccagcgacat
     3541 cgagaccggc agcttctcct tgatcagttc gagcaccgtc gcatcacgga aatagctctt
     3601 gccgaaatca aagcgcatat agtcccacag catcttgaag aaccgctcgt gggccggttt
     3661 gtcgaagccg aactgctttt cgagttcctt gatgaacgcg ggatccagcc cctgtgcgcc
     3721 gcgataggtg gagcttgcct gcgcgctctg caatgccgct tgcccgccga gatcgccggt
     3781 gccgccgccg acccgggatg aggctgaggt atccgccccc tgcaactgcg ccagcacccg
     3841 ctccaccggt ccgcccggtg cgaactgcac gatgacgaac gcgaccagca gaatgccgag
     3901 cagcgtcggg atcatcagaa gcaggcggcg taggatatag gcgagcatga ttcggtttat
     3961 agtccaattc gttaagaaat agggttaacc gatccttgcc tgggatgggg tcggcgatac
     4021 tcctgctcga tcaccgcgac tccccattgc gctcccggtt tctcgtcgac cagggcaaag
     4081 ccgtgcgttt cataaagatg gcaggcggcg tccagcccgg cgaaggtcca cagatgcacg
     4141 gcagcgaatc cggatgcatc gcaatgaccg agcgcggcct ccagcaggcg ccgcccgatc
     4201 ccgctgccgc gcgcttcggc ggcgaccacg aaccaacgca gatgtgcctt gcccggacca
     4261 aggtcctcgc catcgatcgc gatcgtaccg agcagggtat cgccccggtg caccgaccat
     4321 aacccgttca cgggccgctg catccggccc gcgaattcgc tgagtgattc ggcaatgcgc
     4381 gcctcgaaac tcgcgccgaa gccactgagt gcggcataac tcaggccatg cagggcgatg
     4441 caggcgccga tgaggccggg tcgatagccg cgttcaacag gaaggcccgc ttctggcatg
     4501 ggtctggtcc agtcctgccg tctcaaaggc cgatcttctt ggccttctct gcgttgaacc
     4561 accacagccc caccggattg tatccatagc gcgtatcgat cttcggacgg tcgaaaacgt
     4621 cccaataggc gacccgatgc ttgcccgaat accacgccga gacccagtag cgcccggcgc
     4681 gcagaacccg gtcgagcgcg cgcacggcat gggtgaggtc gacgcggctc ttggcgtcga
     4741 tggcggactg gacgagcgca tccaccaccg ggtcggcgat gccggccatg ttgcgcgagg
     4801 cgcgggtgtt ggcatgctcc gagtggaaaa aggacttcag cgattcgccc ggggtttccg
     4861 acatcgagaa ccggcgcgag atcaggtcga aatcgaaatc ctcgacgcgg cgctggaact
     4921 gggtggcatc caccacccgg atcgtcgcct cgatgccgag aagcttgagg ttcttcacaa
     4981 agccttgcag gtgccggttc aggctcggct catcctccat gaactcgatg gtgaacggct
     5041 gaccgcccgg cagcagcagg atgcccttgg ggtcgcgctt gcagcccgcc tcggtcagca
     5101 gctgctgggc cttgcgcaag agggcgcggt cctgccccga tccgtcgctg accggtggca
     5161 catagggctc gccgaacacc tcctccggca ccttgccgcg gaacggttcg agcagtttca
     5221 gttcttcgga cgagggcgga ccgctggcct tcagcgtaga attctcgaaa taggagacgg
     5281 tgcgccggta ggcgtcatac atcaggttct tgttggccca ttcgaaatcg aaggcgaggc
     5341 cgaccgcctc gcgcagccgg cgatccttga attgcggccg gcgggtgttg tgaa
//
LOCUS       DAOMXG010000162         5383 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_8072_length_5383_cov_10.286224, whole genome shotgun sequence.
ACCESSION   DAOMXG010000162 DAOMXG010000000
VERSION     DAOMXG010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 5383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5383
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8072_length_5383_cov_10.286224"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..940)
                     /gene="lptB"
                     /locus_tag="PLQ11_10240"
     CDS             complement(50..940)
                     /gene="lptB"
                     /locus_tag="PLQ11_10240"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HRK25321.1"
                     /translation="MVKTLLQRLRGRVGSAPHRIAPTPPALSDPSGPQAASPGSDTLP
                     SWVQPQARALSGKDILSVHQVGKSYRGREVVRGASLYVRKGESVGLLGPNGAGKTTIF
                     YMITGLVPADKGEIILDGHAITPLPMYRRARLGIGYLPQEASIFRGLTVEQNVRAVLE
                     VVEPDSKKRERELDALLEEFNIARLRKSPSIALSGGERRRCEIARALAGRPSFMLLDE
                     PFAGIDPIAVGDIQALVRQLTARGIGVLITDHNVRETLGLIDRAYIIHSGQVLMEGTA
                     DDIISNPDVRRLYLGEDFRL"
     gene            complement(1006..1656)
                     /locus_tag="PLQ11_10245"
     CDS             complement(1006..1656)
                     /locus_tag="PLQ11_10245"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HRK25322.1"
                     /translation="MILVRSLLPAFIGAAGLVLVASGTDAQTPKPQTASAPASKEAAG
                     KKPASPLSGFGSNSKDPYKIDANRLEVMQKDNSAIYSGDVVVVQDKMTMRCSKLVIFS
                     DQKSSEQRSQAIRGTTSAQPAPAATGPAAASGLKRLECHGPVSIVKEKQTATANMLVY
                     ENDVVTLTGAVVISDGENVQAGEKMVYNTKSGVGTVEGGRVRGIFTPGSGDGKPKT"
     gene            complement(1653..2381)
                     /gene="lptC"
                     /locus_tag="PLQ11_10250"
     CDS             complement(1653..2381)
                     /gene="lptC"
                     /locus_tag="PLQ11_10250"
                     /inference="COORDINATES: protein motif:HMM:NF018537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HRK25323.1"
                     /translation="MVSLAPFRLGLKSVRTAPEPQFGALSPTDQAFEAARRHSVRVRL
                     LRKWIPIACFGAIALTAVGRFLNPFASLPQDVSVSSVALEGSRLTMEQPKLSGFKRDA
                     KAYEVVADSAVQDIRKPNVVELNKPVARIEMQKGTWARLSAKTGVYDATGETLKVTDQ
                     VNIKTDTGLEMKLQRAEIAFKPGTMASDAPAEVVLPNGWVKSDRLSILDHGKVAVFEG
                     NVKSEFIDNAPDAGTSTNDSGPKP"
     gene            complement(2457..3071)
                     /locus_tag="PLQ11_10255"
     CDS             complement(2457..3071)
                     /locus_tag="PLQ11_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HRK25324.1"
                     /translation="MTIRVHQGDLPAPLPSGSVVAIDTETLGLNLNRDRLCVVQLSLG
                     DGTADVVRIAARQTSAPNLAKSLADPTILKLFHFARFDLAVLFRAFGTMPAPVYCTKI
                     ASKLTRTYTDRHGLKDLARELAGIEMSKQQQSSDWGADTLSDAQIAYAASDVLYLHAI
                     KQKLDVMLAREGRADLARAAFAFLPSRALLDLAGWADNDIFAHE"
     gene            3205..4515
                     /gene="guaD"
                     /locus_tag="PLQ11_10260"
     CDS             3205..4515
                     /gene="guaD"
                     /locus_tag="PLQ11_10260"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007196407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HRK25325.1"
                     /translation="MLQKPSLRAVRGRLLWFVDDPAIAGARAYRYLDDGIMIIRNGMI
                     TAIGEHFAMERQMAPGIEMIDTGRHLVMPGFIDAHLHFPQTQVIASYAQGLIDWLNRF
                     TFIEEQRFANPSHARKVAAFFVDALLRNGTTTAAVFGSVHPASAEAFFEAADQRNARM
                     VAGKVMMDRNAPAPLLDTARQAYDETQELIGRWHGHGRQQVAIAPRFAITSTPEQLEA
                     AGALMREHPDCLMMTHLSESRQEIETVASLFPEARDYTDVYDHFGLVGRNSLFGHCIH
                     LGERERLRLAGAGAVAVHCPTSNLFLGSGLFDWQRARDSGMTVAIASDIGAGTSYSML
                     RTLGEAFKVQRLLGHTLDPFAAFHAVTLGNATALRLDQRIGSFAPGREADFVVLDAGA
                     TPEMAHRLERVTSLADELFLLMTMGDERAVRDTYVMGERFLALA"
     gene            complement(4479..>5383)
                     /locus_tag="PLQ11_10265"
     CDS             complement(4479..>5383)
                     /locus_tag="PLQ11_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HRK25326.1"
                     /translation="ERHGAEPREIVGKKLIKKNFPLIHAVGRAAAKEPRLIDITWGRP
                     DDPRVTLVGKGVCFDTGGLNIKPDSAMLLMKKDMGGAATALTAAEMIMGAGLKVRLRV
                     LVPIVENAVAGNAFRPGDVLTSRKGLTVEIGNTDAEGRLILADALALADEETPDLLID
                     FATLTGAARVALGPDLPPFYTDDETLAAEIAEAGRAVNDPVWRLPLWRPYNAMFETRI
                     ADLNNAGTGGMAGSITAALFLKRFVERTPAYAHFDIFGWAPNPRHGQPQGGIPQAARL
                     TFALIERRYGGPRQASARKRSPIT"
BASE COUNT          965 a         1700 c         1687 g         1031 t
ORIGIN      
        1 agagcatgtc ttctgcgatt aaccggtatc cacttaatcg cgacatgctc tagagtcgga
       61 aatcctcgcc gagatagagg cggcgcacgt ccggattgct gatgatatcg tcggccgttc
      121 cttccatcag cacctgaccg gaatggatga tataggcccg atcgatcagg ccgagcgtct
      181 cgcggacatt atggtcggtg atcaggacgc cgatcccgcg ggcggtgagc tgtcgcacca
      241 gcgcctggat atccccgacc gcaatcgggt cgatacctgc aaagggttcg tccagcagca
      301 tgaaggacgg tcggccggcc agcgctcgcg caatctcgca gcggcgccgc tcgccgcccg
      361 acagcgcgat cgacggcgac ttgcgcagtc gtgcaatgtt gaattcctcc agcagggcat
      421 caagttcccg ctcgcgcttc ttcgaatcgg gttcgaccac ctcgagcacg gcacggacgt
      481 tctgctccac cgtcagaccg cggaagatcg aggcttcctg cggcagatag ccgatgccca
      541 gccgcgccct tcgatacatc ggcagcggcg tgatcgcatg gccatcgagt atgatctcgc
      601 ccttatcagc gggcaccagg ccggtgatca tgtagaagat cgtggtcttg ccggcaccat
      661 tggggccaag caggccgacc gattccccct tgcgcacata gaggctggcg ccgcgcacca
      721 cctcgcggcc acggtagctc ttgcccacct gatgaacaga aagaatatcc ttgcccgaca
      781 gcgcgcgcgc ctgcggctgc acccaggaag gcagcgtgtc ggagccaggc gatgcggcct
      841 gcggacctga aggatcggac aaggccggag gcgttggagc gatccggtga ggtgccgatc
      901 cgacgcggcc gcgcagtcgt tgaagaaggg tctttaccac gagtgcatca tcgcatggcg
      961 ccgccgggtc cgtccatccg tcacatgaac gacaggccgg ccgcttcagg tcttgggctt
     1021 gccgtcaccg gaacccggag tgaagatacc gcgcacgcgc cccccctcca ctgtgccgac
     1081 gcccgacttg gtgttgtaga ccatcttctc gccagcctgg acattctcgc catcggagat
     1141 cactaccgcg ccggtcaagg tgacgacatc attctcatag accagcatat tggcggttgc
     1201 ggtctgcttc tccttcacga ttgaaaccgg cccgtgacat tcgagccgct tgagaccgct
     1261 ggctgccgcc ggcccggttg ccgctggcgc gggctgggcc gaggtggtgc cgcgaatggc
     1321 ctggctgcgc tgctcgctgc tcttctgatc agaaaaaatc acaagcttcg agcagcgcat
     1381 cgtcatttta tcctgaacca cgaccacatc gcccgaatag atcgcggaat tgtctttctg
     1441 catcacttca agccggttgg cgtcgatctt gtaggggtct ttggaattgg agccgaagcc
     1501 cgacagcggc gatgccggct tcttgccggc agcttccttg ctggccggcg cgctggccgt
     1561 ctgcggcttg ggggtctgcg catcggtgcc ggaggcaacc agaacgaggc cggcagcgcc
     1621 aatgaaggcc ggaaggagcg aacggacaag gatcatggtt tgggcccact gtcgttggtg
     1681 gaggtcccgg catcgggcgc attgtcgatg aattcgcttt tcacattgcc ttcgaacacg
     1741 gcaaccttgc catggtcaag aatgctcagc cggtcggatt tgacccaacc attgggcagc
     1801 accacttcgg ccggtgcatc cgaagccatt gtgcccggct tgaatgcgat ttcggcccgc
     1861 tgcaatttca tctccagccc ggtatcggtc ttgatattga cctgatcagt cactttcagg
     1921 gtctcgccgg tggcatcata gacccctgtt ttagccgaca ggcgcgccca tgtgcctttt
     1981 tgcatttcga tccgggcgac cggcttgttg agttcgacaa cgttcggctt gcggatgtcc
     2041 tgaaccgccg aatcggcaac cacttcatag gccttggcgt ccctcttgaa gccggacagc
     2101 ttcggctgtt ccatggtcag ccgcgatccc tccagcgcca cgctggagac cgagacatcc
     2161 tgcggcaggc tggcgaaggg gttgagaaag cgccccacgg cggtcaatgc aatggcgcca
     2221 aaacaggcga tcgggatcca cttacgcagc aaccgaacgc gcaccgaatg ccggcgggca
     2281 gcctcgaatg cctgatcggt cggtgacaat gccccgaact gcggctccgg cgcggtgcgg
     2341 accgatttta agccaagccg gaaaggagca agcgaaacca tgctgtccgc taatcctgtt
     2401 tcaatggcga agttatgacg ccttgccgtt aaggacggct gaaggcgcga acctgcctat
     2461 tcatgcgcaa aaatgtcatt gtcggcccag ccggcgagat cgagcaaggc ccggctcgga
     2521 aggaaggcaa aagccgcccg cgccagatcg gcccgcccct cgcgcgccag catgacatcg
     2581 agcttctgct tgatcgcgtg caggtaaagc acgtccgatg cggcataggc gatctgcgcg
     2641 tcgctgagag tgtccgcccc ccagtcggac gattgctgct gcttggacat ctcgatgccg
     2701 gcaagctcgc gtgcaaggtc cttgagaccg tgccggtcgg tataggtgcg tgtcagcttg
     2761 gaggcgatct tggtgcaata gaccggcgcc ggcatggtgc cgaaggcgcg gaacagcaca
     2821 gccaggtcaa accgggcaaa atggaacagc ttgaggatgg tcgggtcggc caggctcttg
     2881 gccaggttcg gcgccgaggt ctggcgggcg gcgatccgca ccacatcggc agtgccatcg
     2941 ccgagcgaca gttgaaccac gcataaacgg tcgcggttca ggttgaggcc gagcgtttcg
     3001 gtgtcgattg ccaccaccga tcccgacggc aggggggcgg gaaggtcgcc ctgatgaacc
     3061 cggatggtca tgaagtccta gaatccctct gaaatcgcct ggccagcttt ctgttctgca
     3121 atagcccgaa gcggcttgcc tcgccaagcc ggcgccgtct cgttcccact aggacagcat
     3181 cgatttctgc gatatgcggc aggaatgctt caaaaacctt cccttcgcgc tgtacgcggc
     3241 cgtctcctgt ggtttgtcga cgatccggcg atcgccggcg cgcgggctta ccggtatctc
     3301 gatgacggga tcatgatcat ccgcaacggg atgattaccg ccatcggcga gcatttcgcc
     3361 atggaacggc agatggcgcc gggcatcgag atgatcgaca ccgggcggca tctggtcatg
     3421 cccgggttca tcgacgcgca tctgcatttt ccccagacgc aggtcatcgc ctcttatgcg
     3481 caagggctga tcgactggct gaaccgcttc accttcatcg aggagcagcg ctttgccaat
     3541 ccttcgcatg cgcgcaaggt ggcggctttc ttcgtcgatg cgttgctcag gaatgggacc
     3601 acgactgctg ccgtgttcgg ctcggtgcat ccggcctcgg ccgaggcgtt cttcgaagcc
     3661 gcagaccagc gcaacgcccg catggtcgcc ggcaaggtga tgatggaccg caatgcccct
     3721 gcccccctgc tcgataccgc ccgacaggcg tatgacgaga cgcaagagct gatcgggcgt
     3781 tggcatggcc atggtcgcca gcaggtggcg atcgcaccgc gctttgccat tacctcgaca
     3841 ccggagcagc ttgaggcggc cggcgccttg atgcgcgagc atcccgattg cctgatgatg
     3901 acacatcttt cggaaagccg gcaggagatc gagacggttg ccagcctgtt tcccgaggcg
     3961 cgcgactata ccgatgtata cgatcatttc gggctggttg gtaggaacag tctgttcggc
     4021 cactgtatcc atctcggtga gcgcgaacgc cttcgtctgg ccggggccgg cgcggtcgct
     4081 gtgcattgcc ccacctccaa tctgtttctt gggtccgggc tgttcgactg gcagagggcg
     4141 cgcgacagcg gcatgacggt ggcgatcgcc agcgacatcg gcgccggcac ctcctattcg
     4201 atgctgcgca cgctcggcga agccttcaag gtgcagcggt tgctgggtca tacgctcgat
     4261 ccgtttgccg cttttcatgc cgtcacgctc ggcaatgcga ccgcgctgcg gctcgatcag
     4321 cgcattggca gtttcgcacc gggacgggaa gccgatttcg tcgtccttga cgccggcgcg
     4381 acacccgaaa tggcgcatcg gctggaacgt gtcaccagcc ttgccgatga attgttcctg
     4441 ctgatgacga tgggtgatga gcgcgcggtg cgtgacacct atgtgatggg cgaacgtttc
     4501 cttgcgctgg cctgacgcgg cccgccatag cgccgctcga tcagtgcgaa ggtcagacgc
     4561 gcagcctgcg gaatgccgcc ctggggctgg ccgtgccgcg gattgggggc ccagccgaaa
     4621 atgtcgaaat gcgcataggc gggcgtccgc tcgacaaagc gcttcaggaa caaagcggcc
     4681 gtgatcgaac cggccatgcc gcccgtgccg gcgttgttga gatcggcgat cctggtttcg
     4741 aacatcgcat tgtaggggcg ccacagcggc agccgccaga ccggatcgtt gacggcacgc
     4801 ccggcttcgg caatctcggc ggccagcgtc tcgtcgtcgg tgtagaacgg cggcagatca
     4861 ggacccaggg ccacgcgcgc cgcgcctgtc agggtcgcaa agtcaatcag caggtcaggg
     4921 gtttcctcat cggccagcgc cagcgcatcg gcgaggatca gacggccttc ggcatcggtg
     4981 ttgccgatct cgacggtcag gcccttgcgg ctggtcagca catcgccggg ccggaaggca
     5041 ttgccggcca ccgcattctc gacgatcggc accagcaccc gcagccgcac cttcagcccg
     5101 gcacccatga tcatctcggc agccgtcagc gcggtcgccg ccccgcccat gtcctttttc
     5161 atcagcagca tggcgctgtc gggcttgatg ttgagcccgc cggtatcgaa acagacgccc
     5221 ttgcccacca gcgtcaccct gggatcgtcc ggccggcccc aggtgatgtc gatcaggcgt
     5281 ggttccttcg ccgctgcacg gccgacggca tggataagcg gaaaattctt cttgatcagc
     5341 tttttgccga cgatttcgcg cggctcggcg ccgtggcgtt cgg
//
LOCUS       DAOMXG010000163         5383 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_8073_length_5383_cov_9.632132, whole genome shotgun sequence.
ACCESSION   DAOMXG010000163 DAOMXG010000000
VERSION     DAOMXG010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 5383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5383
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8073_length_5383_cov_9.632132"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PLQ11_10270"
     CDS             complement(<1..194)
                     /locus_tag="PLQ11_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008193406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HRK25327.1"
                     /translation="MLAQAAKGLLLKEFVSGFGLAMRYFFKPKATLNYPFEKGEISPR
                     FRGEHALRRYPNGEERCIAC"
     gene            complement(194..1270)
                     /gene="nuoH"
                     /locus_tag="PLQ11_10275"
     CDS             complement(194..1270)
                     /gene="nuoH"
                     /locus_tag="PLQ11_10275"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HRK25328.1"
                     /translation="MWAVAWLWTKTTWFVPLLIAALKGLVLLVVLLVLIAFYLLADRK
                     IWAAVQLRRGPNVVGPFGLLQSFADLLKFVLKEPTIPAGSDKILFLLAPLITCVLALA
                     AWAVIPVADGWAIADLNVGVLYVLAISSLGVYGIIIGGWASNSKYPFMAALRSAAQMV
                     SYEVSIGFVIITVLLFCGSLNLNDIVESQNNRYGALGWYIWPLFPMFVIFVISALAET
                     NRPPFDLVEAESELVAGFMTEYGSTPYLLFMLGEYVAIMTMCAMTTILFLGGWLPPIN
                     AAPFTWVPGVFWFLIKVSAVFFIFAMAKAMVPRYRYDQLMRLGWKVFLPLSLAAVVFT
                     AAVLQATGGYRYGVPQVQQTEVKK"
     gene            complement(1383..3446)
                     /gene="nuoG"
                     /locus_tag="PLQ11_10280"
     CDS             complement(1383..3446)
                     /gene="nuoG"
                     /locus_tag="PLQ11_10280"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008190225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HRK25329.1"
                     /translation="MTKLSIDGIEVDVPAHYTLLQAIEAAGIEVPRFCYHERLSVAGN
                     CRMCLVEVKGGPPKPQASCALGVRDIRPGPNGEPPVVLTNSPMVKKAREGVMEFLLIN
                     HPLDCPICDQGGECDLQDQAMAYGGARSRYSEDKRAVEEKYLGPLVKTVMTRCIQCTR
                     CVRFTTEVCGVTEMGAIGRGEDMEITSYLEKALTSEMQGCVVDLCPVGALTAKPHAST
                     YRAWEFAKTESIDVMDALGSAIRVDTRGREVMRVLPRVNDAVNEEWISDKTRHVAEGL
                     RTQRLDRPYVRRDGKLQPASWTEAFAAIAAKVKATQPARIGALAGQLAGVEEMFALKA
                     LMASLGSANMDCRYPGSPLHPNNGRASYLFNSTIAGIEEADGILLIGTNPRIEAPVLN
                     ARIRKRWLRGDVEIGVIGPKADLTYTYQYFGAGPDSIAEFAKVIKGDRKKPMIIVGQG
                     ALSRADGAAILAEIARLAGPLGLIKDGWNGFNVLHTEASLVGALDLGFVPGEGGKDTA
                     GILTPGAVDVIFNLGADEIDIPAGAFVIYQGTHGDRGAHRADVILPGAAYTEKSATYV
                     NTEGRVQVTRRAGFAPGEAREDWAILRALSAVLGQSLPFDSLNQLRAQLYASNRVFAG
                     IDRIAAGKGEDVAALAKLAGKPGKAAFASAITDFYLTNPIARASSVMAECSALAKARG
                     AVAAE"
     gene            complement(3443..3694)
                     /locus_tag="PLQ11_10285"
     CDS             complement(3443..3694)
                     /locus_tag="PLQ11_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25330.1"
                     /translation="MGKEPDNLVLSLLREMRGDLSDIKEKLSDHSRQFKRIRKEMADW
                     QETSATATGFAVHANVRHDGVDKRIDELADRIERLEKAK"
     gene            complement(3698..4999)
                     /gene="nuoF"
                     /locus_tag="PLQ11_10290"
     CDS             complement(3698..4999)
                     /gene="nuoF"
                     /locus_tag="PLQ11_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010686013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HRK25331.1"
                     /translation="MLADRDRIFTNLYGFQDPGLKAALKRGNWDGTKYLLEKGRDWIV
                     DEMKASGLRGRGGAGFPTGLKWSFMPKQNDGRPHYLVINGDESEPGTCKDREILRHDP
                     HLLIEGAMLASFAMGANAAYIYIRGEFIAERVALQKAVDEAYAAKLIGKSNVHGYPFD
                     FYVHHGAGAYICGEETALLESLEGKKGMPRMKPPFPANMGLYGCPTTVNNVESIAVAG
                     TILRRGAGWFNSIGNPNNVGTKLFCVSGHVNKPCNVEEAMGIPFRELIDRHCGGIRGG
                     WDNLKAVIPGGSSVRMVPAAQIIDTPMDFDSLSKLRSGLGTAAVIVMDKSTDVVKAIA
                     RLSAFYKHESCGQCTPCREGTGWMARVIARMAEGRAQKREIDMLLDVSKQVEGHTICA
                     LGDAAAWPIQGLITHFRHEIEAKIDAYAANPHPDPVPMAAE"
     gene            complement(5017..>5383)
                     /locus_tag="PLQ11_10295"
     CDS             complement(5017..>5383)
                     /locus_tag="PLQ11_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH dehydrogenase subunit E"
                     /protein_id="HRK25332.1"
                     /translation="VAVAAPVAAPAKPAAAKAPAEKKASDADKPPVLTAARGGKADDL
                     KLIWGVGPKLEKMLHGMGIFHFDQIAAWTDKQLAWVDENLEGFYGRAERDKWIEQSKK
                     LATGWRPENSVGDKPAGTK"
BASE COUNT          980 a         1792 c         1679 g          932 t
ORIGIN      
        1 ttgcaggcga tgcagcgttc ctcgccgttg ggatagcggc gcagcgcatg ctcgccgcgg
       61 aaacgcggcg aaatctcgcc cttctcgaac ggatagttca gcgtcgcctt gggcttgaag
      121 aaatagcgca tcgccagccc gaagccggag acgaattcct tcagcagcag accttttgca
      181 gcttgagcca gcattatttc ttgacctccg tctgctgaac ctgcggcacg ccatagcgat
      241 agccgccggt tgcctgcaac accgcagcag tgaacaccac cgccgccagt gacagcggca
      301 ggaacacctt ccagcccagg cgcatcaact gatcgtagcg gtagcgcggc accatcgcct
      361 tcgccatcgc gaagatgaag aacaccgccg aaaccttgat gaggaaccag aacacgcccg
      421 gcacccaggt gaacggcgcc gcgttgatcg gcggcagcca gccgccaagg aacaggatcg
      481 tcgtcatggc gcacatggtc atgatcgcca cgtattcgcc gagcatgaac aagagatacg
      541 gcgtcgagcc atattcggtc atgaagccgg cgacgagttc cgattcggcc tcgaccagat
      601 caaacggcgg gcggttggtc tccgccagag ccgagatcac gaagatcacg aacatcggga
      661 acagcggcca gatataccag ccgagcgcgc cataacggtt gttctggctc tcgacgatgt
      721 cgttgaggtt caaggaaccg cagaacagca gcaccgtgat gatgacgaag ccgatcgaca
      781 cctcatagga caccatctgc gccgccgagc gcagcgcggc catgaacgga tatttcgagt
      841 tcgacgccca gccgccgatg atgatgccgt agacgccgag cgaggaaatc gccaggacat
      901 agagcacgcc gacgttgaga tcggcaatcg cccagccatc cgccaccgga atgaccgccc
      961 aggccgcaag cgccagcacg caggtgatca gcggagccag caggaacagg atcttgtccg
     1021 atccggccgg aatggtcggc tccttgagca caaatttcaa aaggtcagcg aaggattgca
     1081 gcaggccgaa cgggcccacc acgttggggc cgcgccgcaa ttgcaccgcc gcccagatct
     1141 tccggtccgc cagcaggtaa aacgcgatca gcaccagcag gacgaccaga agcaccagcc
     1201 ccttgagcgc cgcaatcagc agcggaacga accacgtcgt cttggtccac agccaggcaa
     1261 ccgcccacat ggccaccgcc gcagcaacaa ggactgcaag cccgaggccc agccagatcc
     1321 agagcggagc gatcccgctg ggtttgcgcc gtgccggcgt gacatccacc atcgtcgtcc
     1381 ccttattccg ccgcgaccgc gccacgcgcc ttggcgaggg ccgagcattc ggccatcacg
     1441 ctcgacgcgc gggcaatcgg gttggtcaga taaaagtcgg tgatcgccga agcaaacgcc
     1501 gccttgcccg gcttgccggc gagtttggcc agcgccgcca catcctcgcc cttgcccgcc
     1561 gcgatgcggt cgatgccggc gaacacgcgg ttggaggcgt agagctgggc gcgcaactgg
     1621 ttgagactgt cgaacggcag cgactggccg agcacagccg acagcgccct gaggatcgcc
     1681 cagtcctcgc gcgcctcgcc cggcgcaaag ccggcccggc gcgtcacctg cacgcggcct
     1741 tcggtattga cataggtcgc cgatttctcg gtgtaggccg cgcccggcag gatcacatcg
     1801 gcgcgatggg cgccgcggtc gccatgcgtg ccctgataga tgacgaaggc accggccgga
     1861 atgtcgatct cgtcggcgcc gaggttgaag atcacatcca ccgcgcccgg cgtcaggatg
     1921 ccggcggtat ccttgccgcc ctcacccggc acgaagccca gatcgagcgc gccgaccaga
     1981 cttgcctcgg tatgcagcac attgaagccg ttccagccgt ccttgatcag gccgagcggg
     2041 ccggccagcc tggcgatttc cgcaaggatc gccgcaccat cggcgcggct gagcgcgccc
     2101 tgcccgacga tgatcatcgg cttcttgcgg tcgcccttga tgaccttggc gaattcggca
     2161 atcgaatccg ggccggcgcc gaaatactgg taggtatagg tcaggtcggc cttcggtccg
     2221 atcacgccga tctcgacatc gccgcgcagc cagcgcttgc ggatgcgggc gttgagcacc
     2281 ggcgcctcga tgcgcggatt ggtgccgatc agcaggatgc cgtcagcctc ctcgatcccg
     2341 gcaatggtcg aattgaacag gtagctggcg cggccgttgt tcggatgcag cggcgagccg
     2401 ggataacggc aatccatgtt ggccgaaccg agcgaggcca tcagcgcctt gagcgcaaac
     2461 atctcctcga cgccggcaag ctgaccggcc agagcgccga tgcgcgcggg ctgggtcgcc
     2521 ttcaccttgg ccgcgatcgc cgcaaaagcc tcggtccacg aggcaggctg gagcttgccg
     2581 tccctgcgca catacggccg gtcgagtcgc tgcgtgcgca gtccctcggc gacatggcgg
     2641 gtcttgtcgg agatccattc ctcgttgacc gcgtcgttga cgcgcggcag cacgcgcatc
     2701 acctcgcgac cccgggtatc aacccggatc gccgagccga gcgcgtccat cacgtcgatc
     2761 gattcggtct tggcaaattc ccaggcgcgg taggtcgagg catgcggttt ggcggtcagc
     2821 gcgccgaccg ggcacagatc gaccacgcag ccctgcatct cgctggtcag cgccttttca
     2881 aggtaggagg tgatctccat atcctcgccg cggccgatgg cgcccatttc ggtgacgccg
     2941 cagacttccg tggtgaagcg gacgcagcgc gtgcactgga tgcagcgcgt catcaccgtc
     3001 ttgaccagcg ggccgagata cttttcctcg accgcccgct tgtcctcgga atagcgcgag
     3061 cgcgccccgc cataggccat cgcctggtcc tgaaggtcgc attcgccgcc ctgatcgcag
     3121 atcggacaat cgagcggatg gttgatgagc aaaaactcca tcaccccttc gcgcgccttc
     3181 ttgaccatcg gcgaattggt cagcaccacc ggcggctcgc cattgggacc ggggcggatg
     3241 tcgcgcacgc cgagcgcgca ggaggcctgc ggcttgggcg gaccaccctt cacctcaacg
     3301 aggcacatcc ggcagttgcc ggcgaccgac agccgctcgt gatagcagaa gcgcggcacc
     3361 tcgatgccgg ccgcttcgat cgcctgaagc agcgtgtaat gggcggggac atcaacctcg
     3421 atgccgtcga tggaaagttt ggtcatttcg ccttctccag ccgctcgatc cggtcggcca
     3481 gttcatcaat ccgcttgtcc acgccgtcgt ggcgcacatt tgcatgcacg gcaaaacctg
     3541 tcgctgtcgc gctggtttcc tgccagtcgg ccatctcctt gcggatccgc ttgaactggc
     3601 gggaatggtc ggaaagcttc tccttgatat cggagaggtc gccgcgcatt tcccgcagca
     3661 ggctcagcac cagattgtca ggctccttgc ccatggctca ctccgccgcc atcgggaccg
     3721 gatcggggtg cggattggcc gcataagcgt caatcttggc ctcgatctcg tggcggaaat
     3781 gggtgatcag gccctggatc ggccaggctg ccgcgtcacc cagcgcgcag atggtgtgac
     3841 cctcgacctg tttggacaca tccagcagca tgtcgatctc gcgcttctgc gcgcggccct
     3901 cggccatgcg ggcgatgacg cgggccatcc agccggtgcc ctcgcggcag ggcgtgcact
     3961 ggccgcagct ctcgtgcttg tagaaggccg acaggcgggc gattgccttc accacatcgg
     4021 tcgacttgtc catgacgatc accgccgccg tgccgaggcc ggaacgcagc ttgctgagcg
     4081 agtcgaaatc catcggcgtg tcgatgatct gcgccgccgg caccatgcgc accgacgagc
     4141 cgcccggaat gaccgctttc agattgtccc agccgccgcg gatgccgccg caatggcgat
     4201 cgatcagctc gcggaacgga atgcccatcg cttcctcgac gttgcacggc ttgttcacat
     4261 ggccggagac gcagaacagc ttggtgccga cgttgttggg gttgccgatg gaattgaacc
     4321 agccggcgcc gcgccgcagg atcgtgccgg ccaccgcgat cgattcgacg ttgttgaccg
     4381 tggtcgggca gccgtagagg cccatgttgg ccgggaacgg cggcttcatg cgcggcatgc
     4441 ccttcttgcc ctcaaggctt tcgagcagcg ccgtctcctc gccgcagatg taggcgccgg
     4501 caccgtgatg cacatagaaa tcaaacggat agccgtgaac gttcgacttg ccgatcagct
     4561 tggcggcata ggcttcatcg actgccttct gcaaggccac gcgctcggcg atgaattcgc
     4621 cgcgaatgta gatgtaggcc gcgttcgcgc ccatggcgaa gctggccagc atcgcgccct
     4681 cgatcagaag gtgcggatca tggcggagga tttcgcggtc cttgcaggtg ccgggctcgg
     4741 attcgtcgcc attgatgacg aggtaatgcg ggcggccatc gttctgcttg ggcatgaacg
     4801 accatttgag cccggtcggg aagcccgcgc cgccacgacc gcgcaggccg gacgccttca
     4861 tctcgtcgac gatccagtcc cgaccctttt cgagcagata cttggtgccg tcccagttgc
     4921 cgcgcttgag cgccgccttc aggccggggt cctggaaccc gtagaggttg gtgaaaatgc
     4981 gatccctgtc ggcaagcatc tgtcacattc tcccaatcac ttcgtgccag cgggcttgtc
     5041 gcccaccgaa ttttcagggc gccagccggt ggcgagcttc ttcgactgct cgatccactt
     5101 gtcgcgctcg gcgcggccgt aaaagccttc gagattttcg tccacccagg caagctgctt
     5161 gtcggtccag gcggcgatct ggtcgaaatg gaagatgccc atgccatgca gcatcttttc
     5221 gagcttcggc ccgacacccc agatcagctt gaggtcatcg gctttgccgc cgcgcgccgc
     5281 tgtcagcacc ggcggcttgt cggcgtcgct ggcctttttc tcggccggag ctttggccgc
     5341 agcaggtttg gccggagccg cgaccggggc ggcaaccgcg acc
//
LOCUS       DAOMXG010000164         5371 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_8102_length_5371_cov_9.865124, whole genome shotgun sequence.
ACCESSION   DAOMXG010000164 DAOMXG010000000
VERSION     DAOMXG010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 5371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5371
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8102_length_5371_cov_9.865124"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..1812)
                     /locus_tag="PLQ11_10300"
     CDS             complement(73..1812)
                     /locus_tag="PLQ11_10300"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRK25333.1"
                     /translation="MRRSLFSVLIWPIPLLIGVVGLIVFWQLEHFVERKAVDDVIAED
                     ASHLQAFRKIRTFYSEQVVNKVIAGGRTDVSNQFRGVANTIPYPATFLHEIAGLASDG
                     NHRFSLVSPYPFANRKERTLAPWEKEAWDFLVANPQERYSKLIKIDGRDFIYVAVADR
                     MSSQTCVDCHNSHPDSLKTGWKLGDVRAVFGSTIPLSVISDRLNDVRQWMFGILALTF
                     LLVTVFYIALVRRARNLLLQGIDLLREVVTGRRANLPTTPNRYTETKALNEAAYAYLT
                     AEAERARLERERLADLGATAARARETSESIRTFKGSTGPSLTRLNALGEDLIGKAVAL
                     DRAAGTLSERTAMAMAAAGATSDEVTTARSAAGALFQSIAAMTETSVKAMDVTGMAKA
                     ETMRTQATMQALMDTTARVGMVVESIHAIATQTNLLALNATIEAARAGEAGRGFTVVA
                     QEVKALAAETAKATEQINMEIAAISAACSDASTSFQQVSSVVAEMGRIVGTVSTTSAD
                     QNRWVGTILSNVERVASASEQGVSAVAEVTTATQNVEAVAKELRHLSQDVAADTATLD
                     QDVRVFLASVQTH"
     gene            2011..3315
                     /locus_tag="PLQ11_10305"
     CDS             2011..3315
                     /locus_tag="PLQ11_10305"
                     /inference="COORDINATES: protein motif:HMM:NF028178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRK25334.1"
                     /translation="MKRSYVFLVIALVLGLVGLGAFSFYKFAAPTVLRIAVGPIGSED
                     TKVVATVLQTFAREKHPIRLKLVPTDSLVASADLLKNEKVELAIVRSDANVPPNATTV
                     AIMHRDTMMILAPAGRGIESIAELAGRRIGIGRGVGLNQRLLDLVLERNGIDPANVAK
                     VPVRPTDVRTAMNAGVIDAMLFVGPPTGSQGASAYEALTDDQGRPPVILRVENAEAIV
                     QRIPTLEVSTILRGTFGGAPPRPAENIETIAVTHRLMADRGLKDSVVADLAKALFAVR
                     QSVAAEAPNFARIEAPDTEKLGPLVVHPGAAAYFEGEQKSFIEQYGEWIYISIMGFSL
                     VGSLAAALLSRQFSSARPAGTTEIDRLLLLLRKARLAPDESVLDRLQQEADEVFGRTV
                     ERAAESSIDEAVMSAFTIALGEVRTAIDDRRRILAQEPPIAP"
     gene            3417..5243
                     /gene="typA"
                     /locus_tag="PLQ11_10310"
     CDS             3417..5243
                     /gene="typA"
                     /locus_tag="PLQ11_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HRK25335.1"
                     /translation="MSLRNIAIIAHVDHGKTTLVDKLLAQAGSFRENQRVAERVMDSN
                     DLEKERGITILAKCTSLVWKDTRINIVDTPGHADFGGEVERILGMVDGVVVLVDAAEG
                     PMPQTKFVVGKALKLGLRPIVAVNKVDRSDARAQEVINEVFDLFAALDADDDQLDFPI
                     LYGSGRGGWMAESPEGPQDQGLAPLFDLILRHVPEASTEEGSFRMIGTILEANPFLGR
                     IITGKVVSGSIKPNMPIKALARDGSLIEQGRVSKILAFRGIERTAIDEAVAGDIVAIA
                     GLTKGTVADTFCAPDVTEPLKAQPIDPPTVSMSFIVNDSPLAGTEGDKVTSRVIRDRL
                     LREAEGNVALKIEESADKDSFIVSGRGELQLAILIETMRREGFELAVSRPRVVFQKDE
                     AGQTLEPVEEVFIDVDEEHSGIVVQKMSERKAEMLEMKPSGGHRIRLVFHAPTRGLIG
                     YQSELLTDTRGTAIMNRIFHSYQPYKGEVEGRRNGVLISNDIGEAVAYALWNLEDRGP
                     MVIEPGWKVYRGMIIGIHTRDNDLEVNVLKGKKLTNIRAAGKDEAVRLTPPVRMTLEK
                     ALAWIQDDELVEVTPKSIRLRKMKLDPNERKRDEKAKEALAG"
BASE COUNT          975 a         1635 c         1753 g         1008 t
ORIGIN      
        1 gaaggcccgg ttgcgaggga cggcccggga aatgaccggc aggtcatttg tgaggaccac
       61 tggtatgatc cgtcaatgcg tctgcacgga ggcaaggaag acccgcacgt cctgatcgag
      121 cgtggcggtg tcggctgcaa catcctgcga caggtgacgc agttccttgg cgacggcctc
      181 aacgttctgg gtagcggtcg tcacttcggc gacggccgag acgccctgtt cggaggccga
      241 ggcgacccgt tcgacattcg acaggatggt gccgacccag cggttctgat cggccgaggt
      301 ggttgatacc gttccgacga tgcggcccat ttcggccacg acgctgctga cctgctggaa
      361 agaagtcgag gcatccgagc aggcagccga gatggcggcg atttccatgt tgatctgttc
      421 ggtggccttg gctgtctcgg cagccagcgc cttgacctcc tgcgcgacga cggtgaaccc
      481 gcgcccggct tcgccggccc ttgccgcttc gatggtcgca ttcagcgcca gaaggttggt
      541 ctgggtggca atggcatgga tcgattcaac caccatgccg acgcgggccg tggtatccat
      601 gagggcctgc atggtcgcct gcgtgcgcat tgtctcggct ttcgccatgc cggtgacatc
      661 catcgccttg acagaggttt cggtcatggc ggcgatcgac tggaacagcg cgcccgccgc
      721 gctgcgggcc gtcgtcacct cgtcggatgt ggcgccggca gcggccatgg ccatggcggt
      781 gcgctcggaa agggttcctg cggcgcgatc cagtgcgacc gccttgccga tcagatcttc
      841 accaagagca ttgagtcttg tcagcgacgg tccggttgat cccttgaacg tgcggatgct
      901 ttcgctggtc tcgcgggcgc gtgcggcggt ggctccgaga tcggccagcc gttcacgctc
      961 cagccgtgcc cgttcggcct cggcggtgag ataggcatag gcggcttcgt tcaatgcctt
     1021 ggtctcggta tagcggttgg gcgtcgtcgg caggttggcg cggcgtccgg tcaccacctc
     1081 gcgcaggagg tcgatgccct gcaacagaag gttgcgcgcc cggcgcacca gcgcgatgta
     1141 gaagactgtc accagcaaga aggtcagggc caggatgccg aacatccatt gccggacgtc
     1201 gttcagcctg tcgctgatga ccgatagcgg gatggtgctg ccaaagacgg cgcggacatc
     1261 accaagcttc cagccggtct tgagagagtc cggatggctg ttgtggcaat ccacgcaggt
     1321 ctgcgaactc atcctgtcgg caacggcgac atagatgaag tcgcggccgt cgattttgat
     1381 cagcttggag tatcgctctt gcggattggc aaccaggaag tcccacgcct ccttttccca
     1441 gggtgcgagc gtccgttcct tgcggttggc gaacgggtag gggctgacca gcgagaaacg
     1501 atgatttccg tcgctcgcca gtccggcgat ctcgtgcagg aaggtggcgg ggtaggggat
     1561 tgtgttggct acgccgcgga actggttcga aacatcggtc cgcccgccgg cgatgacctt
     1621 gttgaccacc tgctccgaat agaaggtccg gatcttgcgg aatgcttgga gatggctggc
     1681 atcctcggcg atgacatcgt caaccgcctt gcgctcgacg aagtgctcga gctgccagaa
     1741 cacgatgagc ccgaccactc cgatgagcaa cggaatgggc cagatcagaa cggaaaacag
     1801 actgcggcgc atgaataggc tcggtcatca gggcggagct ggggctccac cgtccggtcc
     1861 aatctagcga cagtgtggtt aagaccctgt gaaatcgcca caattgctgg aacatgccac
     1921 tccatgcggt cgggctgcct tgctcttgcc ggaatgcgcc gctcaagtca ggatgatgtt
     1981 gcctgatttg gcggtactgt gggactggct gtgaagcgct cgtatgtttt tctggtgatt
     2041 gccctggtgc tcggcctggt cggtcttggt gcattctctt tctacaagtt cgcggcgccc
     2101 acggtgctga ggatcgcggt tggtccgatc ggcagcgagg acaccaaggt tgtggccacg
     2161 gtcttgcaga cctttgcccg cgagaagcat ccaatccgcc tgaagctggt gccgaccgac
     2221 agtctggtgg ccagtgccga cctgctgaaa aacgagaagg tcgagcttgc catcgtccgt
     2281 tcggacgcca atgtcccgcc caatgcgacg acggtggcga ttatgcatcg cgacacgatg
     2341 atgatcctgg ctccggccgg tcgcggaatt gaaagcattg ccgagcttgc cgggcgccgc
     2401 attggcattg ggcgcggagt tggcctcaat cagcgcctgt tggatctggt tctggagcgc
     2461 aacggcattg atccggccaa tgttgccaag gttcctgtgc ggccgaccga tgtccgcacg
     2521 gcgatgaatg ccggcgtgat cgatgcgatg ctgttcgtcg gcccgcccac aggcagccag
     2581 ggcgcctcgg cctatgaagc gctgaccgac gaccagggcc gcccgccggt gatccttcgc
     2641 gtcgagaatg ccgaggcgat tgtccagcgc attccgacgc tggaggtgtc caccatcctg
     2701 cgcggcacgt ttggcggggc tccgccgcgc ccggcggaga atattgaaac gatcgccgtt
     2761 acccatcggc tgatggctga tcgcgggctg aaggattcgg tcgttgccga tctcgccaag
     2821 gcgctgttcg ccgtgcgcca gtcggtggcc gccgaagcgc ccaattttgc ccggatcgag
     2881 gctccggaca cggaaaagct cggccctttg gtggtgcatc cgggcgcagc ggcctatttc
     2941 gaaggcgagc agaaatcgtt catcgaacaa tatggcgagt ggatctacat ctcgatcatg
     3001 ggcttttcgc tggtcggctc gctggctgcg gcgctgctca gccgccagtt cagttcggcc
     3061 cgcccggccg ggaccacgga gatcgaccgg ctgctgctgc tgttgcgcaa ggcccgtctt
     3121 gcgccagacg aaagcgtgct ggatcgcctt cagcaggaag ctgacgaggt gttcggccgg
     3181 acggtcgagc gggcggccga atccagcatc gacgaggcgg tgatgagtgc cttcaccatc
     3241 gcgctggggg aggtgcgcac cgccatcgac gaccggcgtc gtattctcgc gcaggaaccg
     3301 cccattgccc cgtaaatatc ggtgttcatt ggttgccctt gccggttgaa acggttattg
     3361 cactgcaaaa ctccactgcg ccgggctgct gtccggcgct tttcgttgag atgtccatgt
     3421 cgctgcgcaa tatcgccatc atcgcccatg tcgaccacgg caagaccacg ctggtcgaca
     3481 agctgctcgc gcaggccggt tccttccgcg agaaccagcg cgtcgccgaa cgggtgatgg
     3541 attccaatga cctcgaaaag gagcgcggca tcaccatcct ggccaaatgc acctcgctgg
     3601 tctggaagga cacccgcatc aatatcgtcg acacccccgg ccacgccgat ttcggcggcg
     3661 aggtcgagcg catcctcggc atggtcgacg gtgttgtcgt gctggtcgat gctgccgaag
     3721 ggccgatgcc gcagaccaag ttcgtggtcg gaaaagcgct gaaactcggc ctgcggccga
     3781 tcgtggcggt caacaaggtc gatcgctcgg atgcccgcgc ccaggaggtg atcaacgagg
     3841 tattcgatct gttcgccgcg ctcgatgccg acgacgacca gctcgatttc ccgatcctct
     3901 acggctccgg ccgcggcggc tggatggccg aaagcccgga aggcccgcag gatcagggcc
     3961 ttgccccgct tttcgatctc attctcaggc atgtgccgga agccagcacc gaggaaggct
     4021 ccttccgcat gatcggcacg atccttgagg ccaacccgtt cctcggccgc atcattaccg
     4081 gcaaggtcgt ctccggctcg atcaaaccga acatgccgat caaggcgctg gcgcgcgatg
     4141 ggtcgctgat cgagcagggg cgggtatcga aaatcctcgc cttccgcggc atcgagcgca
     4201 ctgccatcga cgaggcggtt gccggcgaca tcgtggcgat tgctgggctg accaagggca
     4261 ctgtggccga caccttctgc gccccggacg tgaccgagcc gctgaaggcc cagccgatcg
     4321 atccgccgac cgtgtcgatg agcttcatcg tcaacgattc cccgctcgcc ggcacggagg
     4381 gcgacaaggt gacgagccgc gtcatccgcg atcgcctgct gcgcgaggcg gagggcaatg
     4441 tcgcgctgaa gatcgaggaa tcggccgaca aggacagttt catcgtctcc ggtcgcggcg
     4501 aacttcaact tgccattctg atcgagacca tgcgccgcga gggctttgaa ctcgccgtct
     4561 cgcgcccgcg cgtcgtgttc cagaaggatg aggccggcca gacgctggag ccggtcgaag
     4621 aagtgttcat cgacgttgac gaagagcact ccggcatcgt cgtgcagaag atgagcgagc
     4681 gcaaggccga gatgctggag atgaagccct ccggcggcca tcgcatccgc ctggtgttcc
     4741 acgcgccgac acgcggcctc atcggctacc agtccgaact gctcaccgac acccgcggca
     4801 ccgccatcat gaaccgcatc ttccattcct atcagcccta caagggcgag gtggagggcc
     4861 gccgcaacgg ggtcctcatc tccaacgaca tcggcgaggc ggtggcctat gcgctgtgga
     4921 atctggagga tcgcgggccg atggtgatcg agcctggctg gaaggtctat cgcggcatga
     4981 tcatcggcat ccatacccgc gacaacgatc tcgaagtgaa cgtgctcaag ggcaagaagc
     5041 tgaccaacat ccgcgccgcc ggcaaggatg aggcggtgcg cctgaccccg ccggtgcgca
     5101 tgacgctgga aaaggcgctg gcctggatcc aggacgacga actggtcgag gtgacgccga
     5161 agtcgatccg cctgcgcaag atgaagctcg atcccaacga gcgcaaacgc gacgagaagg
     5221 ccaaggaagc gctggccggc taaagcattt tcaagcgaag tgggttccgg ttcgcgtcaa
     5281 gaaaatgcga aaggccaaaa gagaaagcat tttcaagcgc agtggaattt gctgaaactc
     5341 tgaccgtcag gcggccgtgc cggtcttgac c
//
LOCUS       DAOMXG010000165         5358 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_8126_length_5358_cov_9.776730, whole genome shotgun sequence.
ACCESSION   DAOMXG010000165 DAOMXG010000000
VERSION     DAOMXG010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 5358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5358
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8126_length_5358_cov_9.776730"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /gene="mutL"
                     /locus_tag="PLQ11_10315"
     CDS             complement(<1..1002)
                     /gene="mutL"
                     /locus_tag="PLQ11_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HRK25336.1"
                     /translation="MSVRRLDPVLIDRIAAGEVIERPSAAVKELVENALDAGARRIEV
                     VLEAGGRRLIRVTDDGHGMDAEDLALAVERHATSKLPDGDLTRILTLGFRGEALPSIG
                     SVARLTIATRQAGGANGWALTVDAGRAEPVRPASMGPGTRIEVRDLFYATPARLKFLK
                     SDRAEALAVSQMLKRLAMAQPQTRFILAGDTITGFDFAACEPGAAGQLQRLGQVLGRD
                     FRDNSVPVAAERDGVRLEGFAGLPTYNRATAAEQYLFVNGRPVRDKLLVGAVRGAYHD
                     TMAGDRHAVVALFVALDPAEVDVNVHPAKAEVRFRDPGLVRGLIVGALRLALTGAGHR
                     "
     gene            complement(1059..1547)
                     /locus_tag="PLQ11_10320"
     CDS             complement(1059..1547)
                     /locus_tag="PLQ11_10320"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HRK25337.1"
                     /translation="MMNRRDAVIAASIAAVAGGIAPVLAASGDRVAVEAFYGAFLTAN
                     GQDVAAEGEKVMAPGFESIGDFSGKSKKRDELIQQIQGFHKLVPDLKWQPEEIIEAGN
                     RIVVRSRASGTPRGPLFGVDGKGKSFTLMSIDIHEVVGGRIARVYHVEDWAGALRQLS
                     AQ"
     gene            1653..2000
                     /locus_tag="PLQ11_10325"
     CDS             1653..2000
                     /locus_tag="PLQ11_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018043279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRK25338.1"
                     /translation="MKTPETCPVRGVIDLISDKWSVLVLLHLHHGAMRFTALRRAVPD
                     ISQRVLTATLRKLEREGLIWRSVVPTIPPQVTYGTTELGETLVGHFAALAEWAKHNRP
                     QIDAARQAFDGRS"
     gene            complement(2193..3014)
                     /locus_tag="PLQ11_10330"
     CDS             complement(2193..3014)
                     /locus_tag="PLQ11_10330"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRK25339.1"
                     /translation="MGERMLCRVSVLVFCLLGPVAGAGAETIRVGGTGAVTALIERLA
                     PAFEQASPGDRIVVVPRLGSSGGIRAVSAGALDVAVASRSLTADERASGLNERLFLET
                     PLLFASDTARPLSLTSQDVERIYAGERLSYEDSTLIRLILRPRTDSQNLLLLRTSPGM
                     EVAIGKARLRQDLQVAATDQDNVEIAGATPGAFTAFALTQMRTEPIRLKPVTLNGVEP
                     TLEAMKSGAYPLKVRISWVGKGEAKPAVGRFIAFTGSPEGMAIIEKSGGLPAGQE"
     gene            3094..>5358
                     /locus_tag="PLQ11_10335"
     CDS             3094..>5358
                     /locus_tag="PLQ11_10335"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRK25340.1"
                     /translation="MTGHVSVSHILRSIALAVALLIQTSAPVAFVWHASHDDQQSLAF
                     KARISAGRAAKYILRNLEMWEFHAMRLTEVIEYPGIDATAVRQSILTKDGRVVMAESA
                     ELEWPVISHREPLVVGGQPIGTLLIETSARPTLNHAILVTLAAFVLAVVSFFALHRWP
                     LRMLDRAISRLKAEQARTECALNQLQIADDSLRDRTEQLLAAQKIGRLGDWSLDVETN
                     TPFLSSEMLGLLQIEQAGESPSIDFLLERGVGDSSARMQALVASVIKTGIDGAIDVQV
                     RRTDNSIADFAITCQVASRKGDRVTRLRGIIQDVSERKTAERQLERLAYFDPLTGLAN
                     RSLFQRELRKAVQRARSHDMPAALLLIDLDRFKEVNDSLGHGYGDELLVRVSRLLSQT
                     VPPDQFIARLGGDEFAIILSDISGRAQIAALAETIIDLINKPVQIKRSEALIGASIGI
                     ALLPDHGANAEDLIKNADLALYRAKDAGRGRHMFFEPEMDALMQQKMALARDLRAAAT
                     NDDGLEVWLQPQVSMADHRIVGFEALMRWNHPTRGYIPPTEFIPIAESSSLISDVGLW
                     ILKESAHMAKAWIDAGGEPYEVAVNLSAAQIWQTNIEQDVATVLRESGLPPHLLCLEL
                     TESLLADHAEGRVRNTLTRLKALGVKLALDDFGTGFSSLGYLIQLPFDKLKIDRIFVS
                     QAPRSDKARHVLEGIIALGHGLGMRVLAEGVETPEELDLLRRFGCDEVQGYLFARPSP
                     AAAAYASAINAKVRP"
BASE COUNT         1031 a         1769 c         1654 g          904 t
ORIGIN      
        1 acggtgcccg gcgccggtca gcgccagccg caaggcgccg acgatcagcc cgcgcaccag
       61 cccgggatca cggaagcgca cctctgcctt ggccggatgg acgttgacat cgacctcggc
      121 cggatcgagc gcgacgaaca gcgcgacgac cgcgtgccga tcacccgcca tcgtgtcatg
      181 ataggcgccg cgcaccgcgc cgaccagcag cttgtcgcgc accggccggc cgttgacgaa
      241 cagatattgt tcggccgctg ttgcccggtt gtaggtgggc aggccagcga agccttcgag
      301 ccgcacaccg tcccgttcgg cggcgaccgg cacggaattg tcgcggaagt cgcggccgag
      361 cacctgtccc agccgctgca attgcccggc ggcaccgggc tcgcaggcgg caaaatcgaa
      421 gccggtgata gtgtcgccgg caaggatgaa ccgcgtctgc ggctgggcca tcgccagccg
      481 cttgagcatc tggctgacgg cgagagcttc ggcacggtcg gatttcagga acttgaggcg
      541 ggcgggcgtc gcatagaaca gatcacgcac ctcgatgcgc gtgccgggtc ccatggaggc
      601 cggccgcacc ggttcggcgc ggcctgcatc gacggtcaag gcccagccat tcgccccgcc
      661 ggcttggcgc gtggcaatgg tcaaccgcgc caccgaaccg atcgacggca aagcctcgcc
      721 gcgaaagccg agggtcagaa tgcgggtcag atcaccgtcc ggcagtttgg atgtggcatg
      781 tcgttcaacc gccagcgcca gatcctcggc atccatgccg tggccatcat cggtgacgcg
      841 gatcagtcgc cgcccgccgg cttccagcac cacctcgatg cgccgggcgc cggcatcgag
      901 cgcattctcg acgagttcct tcaccgccgc agacggccgc tcgatcacct cgccggcggc
      961 gatgcggtcg atcaggacag gatcaaggcg acggacggac atgggcgact cacagaaacg
     1021 tcgcccatac cctatgcgca ggcttgaccg ctggcgagct attgcgccga aagctgacgt
     1081 agtgcgccgg cccaatcctc gacatgatag acgcgtgcaa tcctgccgcc gacgacctca
     1141 tggatgtcga tcgacatcag cgtgaaggat ttgcccttgc cgtcgacacc gaacagcggc
     1201 cccctgggcg taccactggc gcgcgagcgg acgacaatcc ggttgccggc ctcgatgatc
     1261 tcctcgggct gccatttcaa atccggcacc agcttgtgaa agccctgaat ctgctggatc
     1321 aattcgtcac gctttttcga cttgccggaa aagtcgccaa tgctctcgaa cccaggcgcc
     1381 atcacttttt cgccctcggc ggcgacatcc tgcccattgg cggtgagaaa ggcgccataa
     1441 aaggcttcaa ccgcgacacg atcgccggat gccgccagca ccggcgcgat gccgccagca
     1501 acagccgcaa tgctcgcggc gatcacggcg tctctacgat tcatcattgg gacttccctt
     1561 cgttcagaac gccttgccat ccggcgtcgg aacagagata ttgcgatggt tacaaaaagg
     1621 aagtaggcac ttttttgata ccggagatgc cgttgaaaac gcctgaaacc tgtcctgtgc
     1681 gcggtgtgat cgacctgatc agcgacaaat ggagcgtgct ggttctgctg catctgcacc
     1741 atggcgcgat gcgcttcact gctctgcgcc gggcagtccc cgatatttcg cagcgcgtgc
     1801 tgaccgcgac tttgcgtaag ctggaacgag agggcctgat ctggcgctca gttgtgccaa
     1861 ccattccgcc gcaggtcacc tatggcacaa cagagctggg ggaaactcta gtcggccatt
     1921 tcgccgcgct tgcggaatgg gccaaacaca accggccaca gatcgacgcg gcgaggcagg
     1981 cgttcgatgg tcggtcgtag gatcccaacg caataaccac gccggctaac ggtcgatcag
     2041 gacaggatca aggcggcgga cggacatggg agcgattcgg gatgagccag acccgggaga
     2101 ctataccagc ccccagcctt tgacctcgca aaagcgcagc ctttccacca tttgccgacc
     2161 gatgcgggcc ctttgtccgt tggaccgggc gatcattcct gcccggcagg cagcccgccg
     2221 ctcttttcga tgatcgccat gccctccggg ctcccggtga aggcgatgaa gcgcccgacc
     2281 gctggtttag cctccccctt gccgacccag gaaatgcgca ccttgagcgg ataggcgcct
     2341 gacttcatcg cttcgagcgt cggctcgaca ccattgagcg tgacgggctt gagcctgatc
     2401 ggctccgtcc gcatctgggt aagcgcaaag gcggtgaagg cgccgggcgt cgccccggcg
     2461 atctccacat tgtcctgatc cgtggcggcg acctgcaaat cctgacgcag ccgcgccttg
     2521 ccgatcgcca cctccatgcc gggcgaggtg cgcagcagga gcaggttctg ggaatcggtc
     2581 cgcggacgca ggatcaaccg gatcaaggtg gaatcctcat aggaaagccg ctcgcccgcg
     2641 taaattcgct cgacatcctg cgatgtcagc gacaagggcc tggccgtatc gctggcaaac
     2701 agcaacggag tttcaaggaa cagccgctca ttgaggccgc tggcccgctc gtccgccgtc
     2761 aaggaccggc ttgcgactgc aacgtccagc gcgccggcac tgacagcgcg aataccgccg
     2821 gaactgccca agcgcggaac gacaacgatc ctgtcgcccg gcgaagcctg ttcgaaagcc
     2881 ggggccaggc gctcgatcag cgccgtgacc gcaccggtgc cgccgaccct gatcgtctcc
     2941 gccccggccc ctgcaacggg acccaggaga caaaatacca gaacggaaac cctgcacagc
     3001 atcctttcac ccatggcaag tccctcgcaa cgaatcattc agaattcaac tataacgtgt
     3061 tttttgtatt ttaatcgacc attgcgggta acaatgaccg gtcacgtcag cgtcagtcac
     3121 attctgcgca gcattgcgct ggccgtggcg ctgcttatcc agacctcggc tccggtcgcg
     3181 tttgtctggc atgccagcca tgatgaccag cagagcctgg cgttcaaggc gaggatcagc
     3241 gccgggcgag ccgccaaata catcctcagg aatctggaga tgtgggaatt ccacgccatg
     3301 cggttgaccg aggtcatcga atatcccggc atcgatgcga ccgctgttcg gcaaagcatc
     3361 ctgacgaagg acgggcgggt tgtcatggcc gaatccgccg agctggaatg gccggtcatc
     3421 agccatcgcg aaccccttgt ggtcggcggc cagcccatcg gcaccctgct gatcgaaacc
     3481 agcgcccgcc caacgctgaa ccatgccatt ctggtgacgc tggcagcgtt cgtgctggct
     3541 gtggtgtcct tcttcgccct gcaccgctgg ccgctgcgga tgctggaccg cgcgatcagc
     3601 aggctgaagg cggaacaggc gcggacagaa tgcgccctca accagttgca gatcgccgat
     3661 gacagcctca gggaccgcac cgagcagctc cttgcagcgc agaaaatcgg ccgcctcggc
     3721 gactggagcc tggatgtcga aaccaacact ccgttcctgt cgagcgagat gctcggactg
     3781 ttgcagatcg aacaggcggg cgagtcgccc agcatcgact tcctgctgga acggggggtg
     3841 ggcgacagca gcgctcggat gcaggcgctg gtcgccagcg tgatcaagac cgggatcgac
     3901 ggcgcgatcg atgtccaggt gcgccgcacc gacaacagca tcgccgattt tgccatcacc
     3961 tgtcaggttg ccagccgaaa gggagatcgc gtcacccggc tcaggggcat catccaggat
     4021 gtcagcgaac gcaagaccgc cgagcgccaa ctcgaacgcc tcgcctattt cgaccctctg
     4081 acgggactgg ccaaccgcag cctgttccag cgggaactca ggaaagccgt gcagcgcgcc
     4141 cgcagccacg acatgcccgc cgcgctcctg ctgatcgatc tcgaccgctt caaggaggtc
     4201 aatgattccc tcggccacgg atatggcgac gaattgctgg tccgcgtgtc gcggctgctg
     4261 tcccagacag tgccgccgga tcagttcatc gcccggctgg gcggtgacga attcgccatt
     4321 atcctctccg acatcagcgg ccgggcacag attgcggcgc ttgccgagac catcatcgat
     4381 ctgatcaaca aaccagtgca gatcaagcgc agcgaggcgc tgatcggggc gtcaatcggt
     4441 atcgccctgc tgcccgatca cggcgccaac gccgaggatc tgatcaagaa cgccgatctt
     4501 gcgctctacc gggccaagga tgccggccgc ggccgtcaca tgttcttcga gccggagatg
     4561 gatgcgctga tgcagcagaa gatggcattg gcgcgcgatc tgagggcggc ggcgaccaat
     4621 gatgacgggc tggaagtctg gctgcagccg caggtcagca tggcggacca tcgcatcgtc
     4681 ggcttcgagg cgctgatgcg ctggaaccac ccgacgcgcg gctacatccc gcccaccgaa
     4741 ttcattccga tcgccgaaag ctccagcctg atcagcgatg tcggcctgtg gatcctcaag
     4801 gagagtgccc acatggccaa ggcatggatc gatgccggcg gcgagcccta tgaggtggcg
     4861 gtcaatctgt ccgccgcgca gatctggcag accaacatcg agcaggatgt cgctacggtg
     4921 ctgcgagaat ccggcctgcc gccgcatctg ctctgtctgg aactgaccga aagcctgctg
     4981 gccgatcatg ccgaaggccg ggtgcgcaac actctgacgc ggctgaaggc gctgggcgtc
     5041 aagctggcgc tcgacgattt cggcaccggc ttctcctcgc tgggctatct gatccagctg
     5101 ccgttcgaca agctgaagat cgaccgcatc ttcgtcagcc aggcgccgcg atccgacaag
     5161 gcgcggcatg tgctcgaagg cattatcgcc ctcggccacg gcctgggcat gcgcgtgctg
     5221 gccgaaggcg tcgagacgcc ggaagaactg gacctgctca gaagattcgg ctgcgacgag
     5281 gtgcagggct atctgttcgc gcgcccttcg cccgccgccg ccgcctatgc cagcgcgatc
     5341 aatgccaagg tccggccc
//
LOCUS       DAOMXG010000166         5289 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_8267_length_5289_cov_10.380971, whole genome shotgun sequence.
ACCESSION   DAOMXG010000166 DAOMXG010000000
VERSION     DAOMXG010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 5289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5289
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8267_length_5289_cov_10.380971"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2377
                     /locus_tag="PLQ11_10340"
     CDS             <1..2377
                     /locus_tag="PLQ11_10340"
                     /inference="COORDINATES: protein motif:HMM:NF025152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR02302 family protein"
                     /protein_id="HRK25341.1"
                     /translation="WPLVGWRWPGLAEAGRRLDLDNRALHRPVTTLGDRPAGDDPVAH
                     AIWERHRTRAETAAAGLTVPPPVSALPQLDGLAIRVLAGLLVVVSAFVAGDERRARLA
                     DALAFGAPLPPPVPPRLDAWVDPPTYTGRAPIFLSGANAPAGGSVSVPAGSILVVRVS
                     PGDGVSLAAPSALKIKEFDPPKDSAGLTAAGKANAQGVFERRLVLDQPASVEVRRGGQ
                     SLGAYDLKTIPDLPPQITFDAIDRDDKTQSLKLKYTLDDDYGIARAEALIGRADGSSS
                     RTLVPPPEVALSTRNGENEAPLVAHEHPWAGVKATIRLRATDDLNQSAESDIREAVLP
                     ARRFDNKVARALIEQRRQIVMFPDNRQTPQIGLDSLLVAPERFTRKSGEFLMLSIAAQ
                     KMRTARTDADLVALAQYLWETAVFLDEGTLTDAERRLRAAEERLREALERGATSDELR
                     QLMDEMRQAMNDMLRDLMERADRNNADRSPNDRNDRNQLTLSQRDLEDMLKRIEELYR
                     SGDTAKAQELLRQLQDLLNNLRSARRREADPRMREMGEALDELDRLQREEEKLRDDTF
                     RNQQRRRMGRNSSPRDPMGQGQQGQRGQRGMQGDSGEDGEEEGDNEGADDKSLAERQQ
                     ALRDRLKQLRDKLRQRGAPTEEGFDGAEQGMGESEQGLRQGQGGRATQGQQQAIDELG
                     RAGRGLAEQMQREMGDGGEPGEGEGLGPGDPRGQQRGRAENNTDPLGRPLPGDRNQLD
                     NSRVRIPQAGEVRGTIAERAQRVLEELRKRFGEIERPREELDYIERLLRRN"
     gene            2435..2626
                     /locus_tag="PLQ11_10345"
     CDS             2435..2626
                     /locus_tag="PLQ11_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HRK25342.1"
                     /translation="MQGVMVFLVPLSIAAVTVVLGLGLFNMLKAGSPSRSQMLMRWRV
                     ILQAVAVALMMLVLWFNMR"
     gene            2631..3209
                     /locus_tag="PLQ11_10350"
     CDS             2631..3209
                     /locus_tag="PLQ11_10350"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012559119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRK25343.1"
                     /translation="MVRLNRIYTRTGDDGTTGLGDGSRIGKDALRVEAYGTIDETNAA
                     IGLARLHLSARPDIDAMLARIQNDLFDLGADLCTPDRGAELGYEPLRIVDSQVVRLEG
                     DIDALNAALQPLKSFVLPAGSPAAAALHLARTICRRAERHMVALAHLPGEIVSAPALR
                     YVNRLSDLLFVAARAANTEQAGDVLWVPAKNR"
     gene            3248..3934
                     /locus_tag="PLQ11_10355"
     CDS             3248..3934
                     /locus_tag="PLQ11_10355"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRK25344.1"
                     /translation="MFLPLNDEAPLPSRFRPLLTWGLIGLTSAIFVLFQSSALFNLEP
                     AVSIGYGLVPAVFSGSARLEDGLAGVPTALTPVSSIFLHGGWMHLITNMLFLGIFGDN
                     IERALGRAGYLLLYLACGAGAGIAYALAYADSTSPMIGASGAVSAVLGAYLVLHPNVR
                     VFGLLLNIIPVRIPAFWFIGGWFALQVGQMLAGDSTGVAWMAHVAGLVLGAVLMAVAR
                     FAVIRRNVGT"
     gene            4087..4836
                     /locus_tag="PLQ11_10360"
     CDS             4087..4836
                     /locus_tag="PLQ11_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017485165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HRK25345.1"
                     /translation="MKIVVPVKRVVDYNVKIRVKPDGSGVDLANVKMSMNPFDEIAVE
                     EALRLKEAGKASEIVVVSIGPAQAQETIRTALAMGADRGILVKTDATVEPLGVAKLLK
                     AVIAKETPGLVIAGKQAIDDDANATGQMLAALLDWRQGTFASKVVIGDGKVDVTREVD
                     GGLQTISLALPAVITTDLRLNEPRYASLPNIMKAKKKPLDETSPEALGVDVAPRLSVL
                     KTSEPPTRKAGVKVADVNELVTKLKTEAGVL"
     gene            4839..>5289
                     /locus_tag="PLQ11_10365"
     CDS             4839..>5289
                     /locus_tag="PLQ11_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HRK25346.1"
                     /translation="MAVLLIAEHDNASLKDATAKALTAAAAIGSPVHVLVAGKGCAGA
                     AKAAAALAGVGKVLLADDAAYDHMLAEATSKLVAGLADGYDAIVAPATANGRNIAPRV
                     AALIDVMLISAITRVVSADTFERPIYAGNAIQTVRSTDAKKVITVQTA"
BASE COUNT          900 a         1672 c         1784 g          933 t
ORIGIN      
        1 gtggccgctg gtcggctggc gctggccggg ccttgccgag gcaggccgcc ggcttgatct
       61 cgacaatcgt gccctgcacc ggccggtgac gacgctgggc gaccggccgg ccggggatga
      121 tccggtcgct catgcgatct gggaacggca tcgtacccgc gccgagactg ccgcagccgg
      181 gctgacagtg ccgccgccgg tcagtgccct gccacagctt gacggcctgg cgattcgggt
      241 gctggccggc ctgcttgtcg tggtctcggc ctttgtcgcc ggcgatgaac ggcgggccag
      301 actggccgat gcccttgcct tcggtgcgcc gctgccaccg ccggtgccgc ctcgcctgga
      361 tgcctgggtt gatcctccga cctataccgg tcgcgcgccg atcttcctgt cgggggccaa
      421 tgcgcctgcc ggaggcagcg tgtcggttcc tgccggcagt atcctggtgg tcagggtgtc
      481 gcccggtgac ggtgtcagcc tggcggcgcc atcggccctg aagatcaagg agttcgatcc
      541 acccaaggac agcgccggcc tgaccgctgc cggaaaggcc aatgcacagg gtgtattcga
      601 gcgccggctc gttctcgatc agccggcgag cgttgaggtg aggcgtggag gccagagtct
      661 tggcgcctat gatcttaaga ccattccgga tctgccgccg cagatcacgt tcgatgccat
      721 cgaccgcgac gacaagaccc agtcgctgaa actgaaatat accctcgacg atgattacgg
      781 cattgccaga gccgaggcgc tgatcggccg tgccgacggg tcctcatccc ggacactcgt
      841 gccgccgccg gaggtggctt tgtcaacccg caacggagaa aacgaggcgc cgctggttgc
      901 ccatgaacac ccgtgggccg gggtcaaggc taccattcgc ctgcgcgcga ccgacgatct
      961 caatcaatcc gcagaaagtg acatccgtga agcagtgctg ccggctaggc gtttcgataa
     1021 caaggtcgcc cgagcactga tcgagcagcg ccggcagatc gtgatgttcc cggacaatcg
     1081 gcaaaccccg cagatcgggc ttgattcact gctcgtcgcc ccggaacgct tcacccggaa
     1141 gtcgggtgaa ttcctgatgc tgtcgatcgc ggcacagaag atgcgcacgg cccgaaccga
     1201 cgcagatctt gtcgcgctcg cccagtacct ctgggaaaca gcggtttttc tcgacgaagg
     1261 cactctgacc gatgccgagc gccgcctgcg ggccgccgag gaacgattgc gtgaggcgct
     1321 ggaacgcggc gccaccagtg acgagttgcg ccagctgatg gacgagatgc ggcaggccat
     1381 gaacgacatg ctgcgtgacc tgatggagcg agccgatcgc aacaacgccg atcgttcgcc
     1441 caatgaccgc aacgaccgta accagctcac cctgtcgcag cgtgaccttg aggacatgct
     1501 caagcgcatc gaggagcttt accgttcggg cgataccgcc aaggcgcagg agcttttgcg
     1561 gcaattgcag gacctgctca acaatctgcg ctcagcgcgc cggcgcgagg cggatccgcg
     1621 catgcgcgag atgggcgagg cactcgatga actcgatcgt ctgcagcgcg aggaggaaaa
     1681 gctgcgcgac gataccttcc gcaaccagca gcgccggcgg atggggcgca attcctcacc
     1741 gcgcgatccg atggggcagg gccagcaggg ccagcgcggc cagcgcggta tgcaggggga
     1801 cagcggcgaa gatggtgagg aagagggcga caacgaaggc gctgacgaca agagcctggc
     1861 tgagcgccaa caggcattgc gcgaccggct gaagcagttg cgcgacaagc ttcgccagcg
     1921 cggcgctccc accgaggagg gattcgacgg cgccgaacag ggaatgggcg aatccgaaca
     1981 ggggttgcgc cagggtcagg gcggacgcgc cacgcaaggc cagcagcagg ccatcgacga
     2041 actgggccga gccggtcgcg gcctcgccga acagatgcag cgcgagatgg gcgacggggg
     2101 cgagccaggc gaaggggagg ggctcggccc aggcgatccg cgcggacaac agcgtggccg
     2161 cgccgagaac aacaccgacc ccctcgggcg cccgctgccg ggagaccgca atcagctcga
     2221 caacagccgc gtcagaattc cgcaggccgg cgaggtgcgc ggcaccatcg ccgaacgcgc
     2281 ccagcgggtg ctggaggaat tgcgcaaacg cttcggcgag atcgagcgcc cgcgtgagga
     2341 actcgactat atcgaacgtc tgctgcggcg gaattgacgg attgccggcg ctgggccaca
     2401 accgcgggga cgtttgtcgc ccaacggagc tgccatgcag ggcgtgatgg tatttcttgt
     2461 gccgctgtcg atcgcggcgg tcacggtggt gctgggtctg gggctgttca acatgctgaa
     2521 ggccggcagc cccagccgtt cgcagatgct gatgcgctgg cgggtcatcc tgcaggcggt
     2581 cgccgtcgcc ttgatgatgc tggtcctctg gttcaacatg cgctgatccc atggtgcgtc
     2641 tcaataggat ctacacccgc acgggcgatg atggcacaac cggcctcggc gatgggtcgc
     2701 gcatcggcaa ggatgccctg cgtgtcgagg cctatggcac gattgacgag accaatgccg
     2761 ccatcggcct ggcgcgactg catctgtccg cccgccccga catcgatgcg atgctcgcac
     2821 gcatccagaa cgacctgttc gatcttggcg cggatctctg cacgccggat cgcggcgccg
     2881 agctgggcta cgagccgctg cggattgtcg acagtcaggt ggtgcggctg gaaggcgata
     2941 tcgatgcgct gaacgcggcg ctgcaaccgc tgaagtcctt cgtcctgccg gccggcagtc
     3001 cggcagcggc cgcgttgcat cttgcccgaa ccatttgccg cagggccgag cggcatatgg
     3061 tggcgctggc gcatcttccc ggcgaaatcg tctccgctcc ggcgttgcgg tatgtcaatc
     3121 ggctgtcgga cctgctgttc gttgctgccc gtgccgccaa tacggagcag gcaggcgatg
     3181 tgctttgggt tccggccaag aaccggtgag cccctttcca tgatggcgca agggaggcgt
     3241 tccgcccgtg tttttgccgc tcaatgacga ggcgccgctt ccctcccgct tccggcccct
     3301 gctgacctgg gggctgatcg ggctgaccag tgcgatcttc gtcctgttcc agagttcggc
     3361 cctgttcaac ctcgagccgg cggtttcgat cggctacggc cttgtcccgg cggtgttttc
     3421 cggctcagcc cgattggagg acggattggc cggtgtgccg actgcgctca ccccggtcag
     3481 ttcgatcttc ctgcatggcg gttggatgca cctgatcacc aacatgctgt ttctcggcat
     3541 tttcggcgac aatatcgaac gggcacttgg ccgggccggc tatcttctgc tctatcttgc
     3601 ctgtggcgcc ggcgccggga tcgcctatgc gcttgcttac gccgattcca ccagtccgat
     3661 gatcggggcc tccggagcgg tttctgccgt gctgggcgcc tatcttgtcc tgcaccccaa
     3721 cgtgcgggtg tttggcctcc tgctcaacat catcccggtg cggattccgg ccttctggtt
     3781 catcggcggc tggtttgctt tgcaggtcgg ccagatgctg gcgggcgatt cgactggcgt
     3841 tgcctggatg gcccatgtcg ccggcctggt tctgggcgcc gttttgatgg cggttgcccg
     3901 tttcgccgtt attcgccgca acgttgggac ctgaccgccg atgtccatgg ccgacttgcg
     3961 ggcggccaat agtttatata tgaaccatta ctgttccata cggacgttgc gttgacgctg
     4021 cctcgccgca tcgttagggt cgcgcggctg ggatttggag cgtttcctaa ggggagttgc
     4081 cgcctgatga agattgtcgt gcccgtgaag agggtggtgg attacaacgt caagatccgc
     4141 gtcaagccgg acgggtccgg tgtcgatctg gcgaatgtca agatgagcat gaacccgttc
     4201 gacgaaatcg ccgtcgaaga ggcgctgcgt ctgaaggaag ccggcaaggc gagcgagatt
     4261 gtcgtcgtgt ccatcggtcc ggcgcaggcg caggagacga ttcgcaccgc gttggcgatg
     4321 ggggccgacc gcggcatcct ggtcaagacc gacgcgacgg tcgagccgct cggtgttgcc
     4381 aagctgctca aggcggtgat cgcgaaggaa acgccgggtc tggtgattgc cggcaagcag
     4441 gcgatcgatg acgatgccaa cgcgaccggc cagatgctgg cggcgctgct ggactggcgg
     4501 cagggcacct ttgcctccaa ggtcgtgatc ggcgacggca aggttgatgt cacgcgcgaa
     4561 gtcgatggcg gcctgcagac catttcgctg gccctgccgg cggtgatcac tacggacctt
     4621 cgcctcaacg agccgcgcta tgcctcgctg cccaacatca tgaaggccaa gaagaagccg
     4681 ctcgacgaga cctctcccga ggcgctcggc gtcgatgtcg cgccgcgcct gtcggtcctc
     4741 aagacgagcg aaccgccgac ccgcaaggcc ggcgtcaagg tggccgatgt caacgaactc
     4801 gtgaccaagc tcaagacaga agcgggagtg ctctgacgat ggccgttctg ctcattgccg
     4861 aacacgacaa tgcgtcgctg aaagacgcca cggcgaaagc gctgactgcc gctgccgcca
     4921 tcggctcacc ggttcatgtg ctggtggccg gcaagggctg cgccggcgcg gccaaggctg
     4981 cggccgctct ggccggcgtc ggcaaggttc tgttggccga cgatgcggcc tatgatcaca
     5041 tgctggccga ggcgacgtcg aaactggtcg cgggcctggc cgatggttac gacgccattg
     5101 tcgcgccggc gaccgccaat ggccgcaaca tcgccccgcg cgtcgcggcg ctgatcgacg
     5161 tgatgctgat ctcggccatc accagggttg tttccgccga cacgttcgag cggccgatct
     5221 atgccggcaa cgcgatccag accgtcaggt cgaccgacgc caaaaaggtg atcaccgtgc
     5281 agaccgccg
//
LOCUS       DAOMXG010000167         5250 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_8351_length_5250_cov_10.310876, whole genome shotgun sequence.
ACCESSION   DAOMXG010000167 DAOMXG010000000
VERSION     DAOMXG010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 5250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5250
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8351_length_5250_cov_10.310876"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="PLQ11_10370"
     CDS             <1..696
                     /locus_tag="PLQ11_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25347.1"
                     /translation="LVGRGRQRRLFGRRRVVRRRRCIGGLVMAMFTAAEQKRVLRAIA
                     EAEKGTSGEIVCVVARAASSYSFAPMAWAAVVALMVPWPLLRFTVLSAERIHLVQLAA
                     FALLAILLAFIPNRAALVPPYFRRRRAHRAALEHFQAQGMVRTAHRTGCLIYVAEAER
                     FAEVLVDEGIAAKVEPAVWRETIEVLTEALRQRSAADGFVKAIALCGSVLKVHAPPRE
                     GDTNEIPDRLILL"
     gene            846..2066
                     /locus_tag="PLQ11_10375"
     CDS             846..2066
                     /locus_tag="PLQ11_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HRK25348.1"
                     /translation="MTPTSQPQTFHHRRAPAEHGHHRVTFLELFFDLVFVFAVTQLSH
                     GLIHHPTWLGLLETAILAMAVWWVWIYTSWCTNWADPDKAPVRMMLFVLMLLGLVMSM
                     AVPKAFEGKAMVFALAYVAMQVGRSAYMLWPMAEFSPGHRRNVLRITLWFVFSGALWI
                     GGAALGGAAQIGLWALAVAHEYAGPAARFWTPGLGASQPGEWDIEPGHFAERCGLFVI
                     IALGESILVASSTFAGQEWRHDNAMAFLATFLGALGMWATYFNIGQERGALVMKRAEN
                     SGLVGRAYTYLHILIVAGIIGTAVSDEFVLAHASGTIDAKTLLATVGGPLLYLIGLML
                     FKRATAGWFPLSHIGGCALLLGLWFAAGALHPWQLALATALVLCLVAAWESWSLGPDA
                     VTKIGAPHQRNKQT"
     gene            complement(2084..2158)
                     /locus_tag="PLQ11_10380"
     tRNA            complement(2084..2158)
                     /locus_tag="PLQ11_10380"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2124..2126),aa:Asn,seq:gtt)
     gene            2378..2451
                     /locus_tag="PLQ11_10385"
     tRNA            2378..2451
                     /locus_tag="PLQ11_10385"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2410..2412,aa:Cys,seq:gca)
     gene            2553..3197
                     /locus_tag="PLQ11_10390"
     CDS             2553..3197
                     /locus_tag="PLQ11_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HRK25349.1"
                     /translation="MTDFSAARRTMVENQIRTYDVSDKLVLAAFEAVPRECFVRPKDA
                     AVAYLDREQMAADGITRLLPPLVLARLMQALELASGQRALDCGSAGYGAALLAAAGLS
                     VVSCEAESEAAAAALARAGISGVEVVDARPGEGAPAKGPFDVILVHGAAEVEPASLLA
                     QLKDGGRLGIIMGLGRAGRASIFRRVGKGFGKARAFDAAGPALQDFARKPEFSF"
     gene            3391..4785
                     /locus_tag="PLQ11_10395"
     CDS             3391..4785
                     /locus_tag="PLQ11_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HRK25350.1"
                     /translation="MAGAVVSVRRLRSVVSAAALVAACLGTSAHAETIHGALARAYHA
                     NPDLNSQRAITRATDEAVPQALSGYRPKVTGAADLGYINQKSHSARQKYSQDDFGPRG
                     LSVTLDQVLYNGGRTGNQLRGAESRVLAARETLRNTEQTILLNAATAYMNVLRDTALL
                     NLRRNNIEVLSTQLKQVSDRFRVGEVTRTDVAQSESRLARARSDAYAAEANLKTSMAT
                     YQQIIGAAPTKLAAARPVENLLPKSREAALAMSQREHPAIIAALHGVDQQQLQVKSVE
                     GELYPTISLSATASRRYDSQAPGSDLWQGSVVGRLSVPIYEGGQVYSRVREAKETLAS
                     QQIVVDSQRERVRQLVVSSWGALEATKAQINAAEAQVRAAEIALAGVREEAKVGQRTT
                     IDVLNSQQDLLDARVSLVTAQRDRVVASYNVLSSVGGLTLRKLGVNVAAYDPKVHYEQ
                     VRDRWTGLRTPDGQ"
     gene            4910..>5250
                     /locus_tag="PLQ11_10400"
     CDS             4910..>5250
                     /locus_tag="PLQ11_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25351.1"
                     /translation="MSSAAASVPPPPPRAAEPSMEEILASIRKIISEDQKVLEARADA
                     ARNPELGSAEPVDDADSAVGEDDQDVLELPASAMRPQKSAPDVVPAAASAAPVQPEPV
                     VAPVFVAPVSVA"
BASE COUNT          857 a         1614 c         1787 g          992 t
ORIGIN      
        1 ctggtcgggc gggggcgaca gcggcggcta ttcgggcggc ggcgggtcgt ccggcggcgg
       61 cggtgcatcg ggggattggt gatggcaatg ttcaccgcag ccgaacagaa acgcgtcctc
      121 cgcgccattg ccgaggccga gaagggcacg tcgggcgaaa tcgtctgtgt ggtcgcccgt
      181 gcggccagca gctacagttt cgcgccgatg gcatgggcgg cagtggtggc actgatggtg
      241 ccgtggccgc tgttgcggtt caccgtgctt tcggcggaac ggatccacct tgtccagctt
      301 gccgcctttg ccttgctggc gatcctgctg gcattcatac ccaaccgggc ggcgctggtt
      361 ccgccctatt tccggcggcg gcgcgcgcat cgggcggcgt tggagcattt ccaggcgcag
      421 ggcatggtgc gcacagccca tcgcaccggc tgcctgatct atgtggcaga ggccgagcgc
      481 tttgccgaag tgctcgtcga tgaggggatc gcggcgaaag tggagcccgc tgtctggcgc
      541 gaaaccatcg aggtgttgac cgaggctctg cggcagcgct ccgctgccga cgggttcgtc
      601 aaggcaatcg cgctgtgcgg cagcgtgttg aaagtccatg ccccaccgcg tgaaggcgat
      661 accaacgaga taccggaccg gctgatcctg ctgtaggcta gatcacgccg cattttgatg
      721 gaatcatcaa aatgcgtata acgtgatcgg tatcaataag ttagagcatg tcttctgcga
      781 ttaaccggta tccacttaat cgcgacatgc tctagccata ctgactctga tccggatccg
      841 catgcatgac cccgacatcg cagccccaga ccttccacca ccgccgcgcg ccggccgagc
      901 acggccacca tcgggtgacg ttcctcgaac tgttcttcga tctggtgttt gtctttgcgg
      961 tcacccagct ttcgcacggg ctgatccacc atccgacctg gctcggcctg ctggaaaccg
     1021 ccatcctcgc gatggcggtg tggtgggtgt ggatctacac ctcgtggtgc accaactggg
     1081 ccgatccgga caaggcgccg gtgcgcatga tgctgttcgt gctgatgctg ctcggactgg
     1141 tgatgtcgat ggctgtgccc aaggcgtttg agggcaaagc gatggtgttc gcgctcgcct
     1201 atgtggcgat gcaggtagga cggtcagcct atatgctgtg gccgatggca gaattctcgc
     1261 cggggcatcg ccgcaacgtt ctcaggatca ccctctggtt cgtgttttcc ggtgcgctgt
     1321 ggatcggggg ggcggcgctc ggcggagctg cgcagatcgg cctgtgggcg ctggcggttg
     1381 cgcatgaata tgccgggccg gccgcacgtt tctggacgcc ggggcttggc gcgtcacagc
     1441 cgggagaatg ggatatcgaa ccggggcatt ttgccgaacg ctgcgggctg ttcgtcatca
     1501 tcgcgctggg cgaatcgatt ctcgtcgcca gttccacttt cgccgggcag gagtggcggc
     1561 acgacaatgc catggcgttt ctggcgacct ttctcggcgc gctggggatg tgggcaacct
     1621 atttcaacat cgggcaggag cgtggcgcac tggtcatgaa gcgggccgaa aattccggcc
     1681 tcgtcgggcg ggcctacacc tatctgcaca tcctgatcgt tgccggaatc atcggaacgg
     1741 cggtgtcgga tgaatttgtc ctcgcccatg ccagcggcac gatcgatgca aagaccctgc
     1801 tggccaccgt gggcgggccg ctgctgtatc tgatcgggct gatgctgttc aagcgggcca
     1861 cagccggctg gttcccgctg tcgcatatcg gcggctgtgc gctgctgctc ggactgtggt
     1921 tcgccgccgg cgcgctgcat ccctggcaac tggcgctggc gaccgcactg gtgctgtgcc
     1981 tcgtggctgc ctgggaaagc tggtcgctcg gccccgatgc agtgaccaag atcggcgctc
     2041 cgcaccaacg gaacaagcag acctgagcga tcgaaaagag aaatggctcc ccgagtagga
     2101 ttcgaaccta cgaccattcg attaacagtc gaatgctcta ccgctgagct atcggggaat
     2161 gcggcccgcc gtatagccgc agccgcagcg cgatgcaatc gaaaacgaaa aaaactgtcg
     2221 ggcgcctgtg cgaacgcccg caacggtgcg gcagcctgcc gcatgtgccg cagcgtcggg
     2281 tggccatgca atgtcctgat gcaatttctt caccggttcg atgcaatggt gattgcaatc
     2341 cgttcgtcag tagttatacc ggcgacgcgc ttcgattggc ctcgtggcgg aatggttacg
     2401 cagaggactg caaatccttg tatcccagtt cgattctggg cgaggcctcc acttcccccc
     2461 aaatgttgat cccgccctaa ggctgcggcc ttgcatttcg gcgctgtctg ccgcaatagc
     2521 aacggatcag ccctgcaacg tttggccccg ccatgaccga tttttccgcc gcccgccgca
     2581 cgatggtcga aaaccagatc cgcacctatg acgtgtcgga caagctggtg ctggcggcgt
     2641 tcgaggcggt gccgcgtgaa tgcttcgtcc ggccgaagga tgcggcagtg gcctatctcg
     2701 accgcgagca gatggccgcc gatggaatca cccgcctgct gccgccgctg gttctggcgc
     2761 ggctgatgca ggcgctggag cttgcttcgg gccagcgggc gctcgattgc ggcagcgccg
     2821 gctatggcgc ggcgctgctg gcggcggccg ggctctcggt tgtctcctgc gaagcggaat
     2881 cggaagcggc agccgctgct ctggcccggg cggggatctc cggcgtcgag gtggtcgatg
     2941 cgaggccggg cgagggtgct ccggccaagg gtccgttcga cgtcattctg gtgcatggcg
     3001 ccgctgaagt cgagccggca agccttctcg cccagctcaa ggatggcggg cggctgggca
     3061 tcatcatggg gctggggcgg gcgggccgcg ccagcatctt ccgccgcgtc ggcaagggtt
     3121 ttggcaaggc gcgcgctttc gacgctgccg gaccggcctt gcaggatttt gcccgcaagc
     3181 cggaattcag cttctgacct gacgatttcc ggcggcggcc agccagcgcc ggattgtgga
     3241 atgcacccgg cgggacgggc agatgatgcg cgtttccagc aacactgccg gcgcaaacat
     3301 gagcattttg tcatttgact ggggacgcgc tccggcaaac atttacacgc cgtgctgaat
     3361 cgtaaacaaa tcgtaaagga cctctttgga gtggcgggtg ctgtagtgtc ggtgcggaga
     3421 ttgcgttctg tggtgtcggc tgcggccctg gtggccgcgt gccttggcac gtcggcgcat
     3481 gccgagacca ttcatggggc gctggcccgt gcctatcatg ccaacccgga tctgaactcg
     3541 cagcgcgcga tcacccgcgc gaccgatgag gcggtgccgc aggcgctgtc cggctatcgg
     3601 ccgaaggtga ccggtgcggc cgatcttggc tatatcaatc agaagtcgca ttcggcgcgg
     3661 cagaaatatt cgcaggatga tttcggcccg cgcggcctgt cagtgacgct cgatcaggtg
     3721 ctgtacaacg gcgggcgcac cggcaatcag ctgcgcggcg cggagtcacg cgtgctggcg
     3781 gcgcgggaga cgttgcgcaa taccgagcag accattctgc tcaatgccgc caccgcctat
     3841 atgaatgtgc tgcgcgatac cgcgctgctc aatcttcgcc gcaacaatat cgaggttctg
     3901 tcgacccagc tcaagcaggt gagcgaccgc ttccgggtcg gcgaggtgac ccgcacggat
     3961 gtggcgcaat cggagtcgcg gctggcccgc gcccgttcgg atgcctatgc tgccgaggcc
     4021 aatctcaaga cgtcgatggc cacctatcaa cagatcatcg gcgccgcacc gaccaaactg
     4081 gcagcggcca ggccggtcga gaacctgctg ccgaagtcgc gtgaggcagc gctggcgatg
     4141 tcgcagcgcg aacatccggc gatcattgcc gcgctgcacg gcgtcgatca gcagcagttg
     4201 caggtcaagt cggtcgaggg cgagctttat ccgacgatca gcctctcggc gacggccagc
     4261 cggcgttacg acagccaggc tcccggctca gacctgtggc agggctcggt ggtggggcgc
     4321 ctgtcggtgc cgatctacga gggcgggcag gtctattccc gggtgcgcga ggccaaggaa
     4381 acgctcgcct cgcagcagat cgtcgtcgat tcgcagcggg agcgtgtgcg gcagctggtg
     4441 gtcagttcat ggggcgccct ggaagcgacc aaggcgcaga tcaacgcggc cgaggcgcag
     4501 gtgcgtgccg ccgagatcgc cctcgccggc gtgcgggaag aggccaaggt gggccagcgc
     4561 acgacgatcg acgtgctcaa ttcgcagcag gacctgctgg atgcgcgggt gtcgctggtg
     4621 acggcgcagc gcgaccgggt ggtggcatcg tataatgtgc tgtcatcggt cggcggtctg
     4681 acgctgcgca agctcggcgt caacgtcgca gcctatgacc ccaaggtgca ttacgagcag
     4741 gtgcgcgacc gttggaccgg gctgcgcacg ccggacgggc aatagagccc ccctccatcc
     4801 actgccaaat tgcggtgacc gcttgcccag caacggcacg ctgctctact tctaagcgca
     4861 gggctggcat tgccggccgg atgcctgatc cttgcctttg gagattgtca tgagttctgc
     4921 cgccgccagt gttccgccgc cgcctccgcg cgccgccgag ccgtcgatgg aggaaatcct
     4981 ggcctcgatc cgcaagatca tttccgagga tcagaaggtg ctggaggccc gcgccgatgc
     5041 tgcgcgcaat cccgagctgg gatcggcgga gccggtcgac gacgccgatt ccgcggtggg
     5101 ggaggacgac caggatgtgc tggagcttcc cgcctcggcc atgcgcccgc agaagagcgc
     5161 acctgacgtg gtgcctgcgg cggcgtctgc ggcgccggtc cagcctgagc ccgttgtggc
     5221 gcctgtcttc gtggcgccag tatccgtagc
//
LOCUS       DAOMXG010000168         5240 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_8381_length_5240_cov_10.163549, whole genome shotgun sequence.
ACCESSION   DAOMXG010000168 DAOMXG010000000
VERSION     DAOMXG010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 5240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5240
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8381_length_5240_cov_10.163549"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="PLQ11_10405"
     CDS             complement(<1..636)
                     /locus_tag="PLQ11_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018262053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRK25352.1"
                     /translation="MDKARVLLFDHALTPDGWARDVALHLEGGRIVSVVPQAGLPAGA
                     ERIAGAALPGLPNLHSHCFQRGMAGLAERRGPAADSFWTWRQVMYRFLERLDPEDVEA
                     IAAMAMVEMLEGGFTSLAEFHYLHHGPDGVPYDDPAELAGRIVSAACQTGIGLTLLPV
                     FYAHGGFGGAAPAPGQRRFITTPDRFLALHAAAARAVAACDGARIGVAPHSL"
     gene            803..2008
                     /gene="hutI"
                     /locus_tag="PLQ11_10410"
     CDS             803..2008
                     /gene="hutI"
                     /locus_tag="PLQ11_10410"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009489349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HRK25353.1"
                     /translation="MFDTLWINARLATMSADTPEGLGLIDEGALGCRDGRIVYAGPRA
                     QLPSLAAAETIDCSGRLVTPGLIDCHTHLVYGGHRAREFELRLKGASYEEIARAGGGI
                     LSSVAATRAASGAELTQGALLRLDALIAEGVTTIEIKSGYGLDTETESRCLAIARSLP
                     ALRQIRVATTFLGAHAMPPEAGGDKDGYIDRLIAEMLPGLARDGLVDAVDAFCEGIGF
                     SPEQVGRLFAAAEALGLRVKLHADQLSNLGGAALAARFRALSADHLEYTDEAGAQAMA
                     AAGTIAVLLPGAFYFLRETRKPPVELFRRHGVAMALATDSNPGSSPLTSLLLTMNMGA
                     TLFRMTVAECLAGVTRHAAAALGLAAETGTLEVGKSCDLAIWDVREPAELVYRIGFNP
                     LHRRIWRGA"
     gene            2005..3576
                     /gene="hutH"
                     /locus_tag="PLQ11_10415"
     CDS             2005..3576
                     /gene="hutH"
                     /locus_tag="PLQ11_10415"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HRK25354.1"
                     /translation="MTMVTLQPGGVPLATWRAIYRGAGVTLDPACHAAVARSADCVAA
                     IVAKGDPVYGINTGFGKLATVRIGADDLSHLQRNIVLSHAAGVGEPTPAAVVRLMMAL
                     KLASLAQGASGVRVATLTLLQALLARNLIPLVPSQGSVGASGDLAPLAHMTAAMLGVG
                     FVMTDHGIVPAAEALKQAGLAPAELGPKEGLALLNGTQFSTAFALAGVFEAEALFQAA
                     LMTGALATDAARGSDAPFDPRIHRLRRHCGQIETAAALRQLMQGSAIRASHLVGDERV
                     QDPYCLRCQPQVMGAVLDILRQAATTLETEANGVSDNPLIFSETGEALSGGNFHAEPV
                     AFAADMIALALCEIGSLAERRIAMLVDPALSGLPAFLVARPGLNSGFMIPQVTAAALV
                     SENKQRATPASVDSLPTSANQEDHVSMAAHGARRLMPMAENLRAILAIELLAAAQGCD
                     FHAPLLSSPALEAVRSALRAKVPSLDVDRYLHPDLQAAIALIGAGQIIAAAGSIALPG
                     LAQPTDQPPNIARTP"
     gene            3573..5237
                     /locus_tag="PLQ11_10420"
     CDS             3573..5237
                     /locus_tag="PLQ11_10420"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HRK25355.1"
                     /translation="MTRIDNARIIRAPHGTDISAKSWMTEAPLRMLMNNLDPDVAEKP
                     GELVVYGGIGRAARDWESFDRICAALRRLEADQTLLVQSGKPVGVFRTHADAPRVLIA
                     NSNLVPHWANWDHFNELDRKGLMMYGQMTAGSWIYIGSQGIVQGTYETFAEMGRRHYG
                     GSLMGRWILTAGLGGMGGAQPLAATMAGASCLAVECRPSSIEFRLRTGYVDCQAKDLD
                     DALAILDRAKADGKAVSVALLGNAADVFPEIFRRGIRPDCVTDQTSAHDPVHGYLPKG
                     WTLAQWEAMQASDPKAVTAAAKKSMAVHVRAMLDFHKAGVPTVDYGNNIRQMALEEGV
                     TDAFDFPGFVPAYIRPLFCRGIGPFRWAALSGDPEDIWRTDAKVKELIPDNRHLHNWL
                     DMAQRRIRFQGLPARICWVGLGDRHRLGLAFNEMVAKGELKAPIVIGRDHLDSGSVAS
                     PNRETEAMMDGSDAVSDWPLLNALLNCASGATWVSLHHGGGVGMGFSQHSGMVICCDG
                     TPEAARRIVRVLWNDPATGVMRHADAGYDIARDCARTHRLDLPSLA"
BASE COUNT          863 a         1747 c         1706 g          924 t
ORIGIN      
        1 cagcgaatgc ggcgcaaccc cgatccgcgc gccatcacag gccgccaccg cgcgggccgc
       61 cgccgcatgc agcgccagga accgatcagg cgtggtgata aaccgccgct ggcccggcgc
      121 cggcgccgct ccgccaaaac cgccatgagc atagaatacc ggcagcagcg tcaggccaat
      181 gcctgtctgg cacgcagcgc tgacgatccg cccggcgagt tcggccggat cgtcataagg
      241 cacgccgtcc ggcccgtgat gcaggtaatg gaattcggcg aggctggtga acccgccttc
      301 cagcatttcc accatcgcca tcgcggcaat cgcctcgaca tcctccggat caagccgctc
      361 gagaaagcga tacatgacct gccgccaagt ccagaagctg tcggctgccg gcccgcgccg
      421 ctcggcaagg cccgccatgc cgcgctggaa gcaatggctg tgcagattgg gcaggcccgg
      481 cagggcggcc ccggcaatcc gttccgcacc ggctggcaat cccgcctgcg gaacaaccga
      541 gacgatccgc ccaccctcaa ggtgcagcgc gacatccctc gcccagccat ccggcgtcag
      601 cgcatggtcg aacagcagca cgcgcgcctt gtccaacacc ctctccctgc ctctgccggc
      661 aggatggcat gtgccgggaa cgatggcaat atgtctatac aatagattcc gccaataacc
      721 ttattgcagc tgcgaagggc caacttgggc ctttaccacc gctgtttgcc gtaatatgtc
      781 tatacatata aggggggggg cgatgttcga tacgctctgg atcaacgcgc gtctggcaac
      841 catgtcagcg gatacgcccg aggggcttgg tctcattgac gagggcgcgc tcggctgccg
      901 cgacgggcgc atcgtctatg ccggcccgag ggcgcagctg ccctccctag ccgctgcaga
      961 gacgatcgac tgctccgggc gcctggtgac gcccggcctg atcgattgcc acacccatct
     1021 ggtctatggc ggtcatcgcg ccagggaatt cgaactcagg ctcaagggtg cgagctatga
     1081 ggagatcgcc cgcgccgggg gcggtatcct gtcgagcgtc gccgcaaccc gcgctgccag
     1141 cggcgcagaa ttgacgcaag gcgcattgct ccgcctcgat gcgctgatcg ccgagggtgt
     1201 caccaccatc gagatcaaat cgggctacgg gctcgacacc gagacggaga gccgctgcct
     1261 ggccattgcc cggtcattgc cggccttgcg gcaaatccgg gttgccacaa cgtttctcgg
     1321 cgcccatgcc atgccgccgg aagcgggagg cgacaaggat ggctacatcg accgtttgat
     1381 cgccgagatg ctgcccgggc tggcacgaga cggcctcgtc gatgcggtgg atgccttctg
     1441 cgaaggcatt ggcttttcac ccgaacaggt cgggcggctg tttgcagcgg cagaggctct
     1501 gggccttcgg gtgaagctgc atgccgatca attgtccaac cttggcgggg cggcgctggc
     1561 ggcgcgattc cgggcccttt ccgccgatca tctcgaatac accgatgaag ccggtgcgca
     1621 ggccatggct gcggccggta ccattgccgt tctgttgccc ggcgccttct acttcctgcg
     1681 cgagacccgc aaacctccgg tcgaattgtt ccgccgccat ggcgttgcca tggcgctggc
     1741 caccgacagc aacccgggct cctccccgct gacttccctg ctgctgacga tgaacatggg
     1801 ggcgacattg ttccgaatga ccgtcgccga atgccttgca ggcgttacgc gccatgccgc
     1861 cgcagcgctc ggccttgcgg cagaaaccgg cacgctggag gttggcaagt cgtgcgatct
     1921 ggccatctgg gacgtgcgcg agccggccga acttgtctat cgtatcgggt tcaacccgct
     1981 acatcgccgc atctggaggg gtgcatgacc atggtgacgt tacagccggg cggggtgccg
     2041 cttgccacct ggcgggcgat ctatcgcggc gccggcgtga cgctcgatcc cgcctgccat
     2101 gcggccgtcg ccagaagcgc ggattgcgtc gccgcgatcg ttgccaaggg tgaccccgtc
     2161 tatggcatca acaccggctt tggaaagctc gcgaccgtcc gcatcggggc cgacgatctc
     2221 agccatctgc aacgcaacat cgtcctcagc catgcggcgg gggttggcga accgacgccg
     2281 gcagctgtcg tccggctgat gatggcgctg aagcttgcca gtctggccca gggcgcatcc
     2341 ggcgtgaggg tggcgacgct caccctgttg caggcccttc tggcacgcaa cctcatcccc
     2401 ctggtgccgt cgcagggttc ggtcggcgct tcgggcgatc tggcgccgct ggcccatatg
     2461 accgcagcga tgctgggtgt cggcttcgtc atgacggacc atggcatcgt gccggcggcc
     2521 gaagccttga aacaggccgg gctggcgccg gccgagcttg gccccaagga agggctggcg
     2581 ttgctcaacg gcacgcaatt ttcgacagcc tttgccctcg ccggtgtgtt cgaggccgag
     2641 gcactgtttc aggcggccct gatgaccggc gcgctggcaa ccgatgcggc aagagggtcg
     2701 gacgccccgt tcgatccccg catccatagg ctgcgccggc attgcggcca gatcgaaacc
     2761 gctgcggcct tgcggcagct gatgcaggga tcggccattc gcgcttcgca tcttgtcggc
     2821 gacgagcgcg tgcaggatcc ctattgcctg cggtgccagc cacaggtgat gggcgccgtg
     2881 ctcgacatcc tccggcaggc cgcgacgaca ctcgaaaccg aggccaacgg cgtttcggac
     2941 aatccgttga tcttctccga gacaggcgaa gcgctgtccg gcggcaattt ccatgccgag
     3001 ccggtcgcct ttgctgccga catgatcgcg ctggcgctgt gcgagatcgg ctcgctggcc
     3061 gagcggcgca tcgccatgct ggtcgatccg gcgctgtccg gcctgcctgc cttcctggtg
     3121 gccaggccag gcctcaattc gggcttcatg attccgcagg tgaccgccgc cgcgctggtc
     3181 agcgagaaca agcagcgggc aaccccggcg agtgtcgatt ctctgcccac ctccgccaat
     3241 caggaggatc acgtttcgat ggcggctcac ggcgcgcggc ggctgatgcc aatggccgag
     3301 aacctcaggg ccatcctggc catcgaactg cttgccgcag cgcagggttg tgatttccat
     3361 gcgccgctgc tgtcgagccc ggcgctcgaa gcagtgcgtt cggcactgag agcaaaggtg
     3421 ccaagcctcg acgtggatcg ttatcttcat ccggacctgc aagcggccat cgcgctcatt
     3481 ggcgcgggac agatcatcgc cgccgccggc tcgattgcgc tgccgggact ggcgcagcca
     3541 acggaccagc cgcccaacat agcgaggacc ccatgacccg tattgacaac gcccgcatca
     3601 tccgagctcc ccatggcacg gatatcagcg cgaaaagctg gatgaccgag gctcctttgc
     3661 gcatgctgat gaacaatctt gacccggatg ttgccgaaaa gccgggcgaa ctggtcgtct
     3721 atggcggcat cggccgggcg gcgcgcgact gggagagctt tgaccgcatc tgcgccgcat
     3781 tgcggcggct ggaggcggat cagacgctgc tggtgcaatc gggcaagccg gtgggcgtgt
     3841 tccggaccca tgccgatgcg ccgcgagtgc tgatcgccaa ttccaacctc gtgccgcatt
     3901 gggccaactg ggatcatttc aacgaactcg atcgcaaggg cctgatgatg tatggccaga
     3961 tgacggccgg ttcctggatc tacatcggct cccagggcat cgtgcagggt acctacgaaa
     4021 ccttcgccga aatggggcgc cggcattatg gcggctcgct catgggccgc tggatcctca
     4081 ccgccggtct cggcggcatg ggcggggcgc agccgctggc ggcgaccatg gcgggggctt
     4141 cctgccttgc ggtcgagtgc cgcccctcgt cgatcgagtt ccgcttgcgg accggttatg
     4201 tcgattgcca ggccaaggac ctcgacgacg ctctggcgat cctcgaccgc gcgaaggcgg
     4261 acggcaaggc cgtctcggtc gcgctgctcg gcaatgccgc cgatgtcttt ccggagattt
     4321 tccggcgcgg catccgccct gattgcgtga ccgaccagac ctcggcacat gatccggtgc
     4381 atggctacct gccgaagggc tggacgctgg cgcaatggga agcaatgcag gcctccgacc
     4441 ccaaggcggt caccgctgcg gcgaaaaagt cgatggcggt gcatgtgcgt gccatgctcg
     4501 atttccacaa ggcgggcgtg ccgacggtcg attacggcaa caacatccgc cagatggccc
     4561 tggaagaagg cgtcaccgac gccttcgatt tccccggctt cgtgccggcc tacatccggc
     4621 cgctgttctg tcgggggatc ggtccgttcc gctgggccgc cctgtccggc gatcccgagg
     4681 atatctggcg caccgatgcc aaggtgaagg aactgatccc tgacaatcgg catctgcaca
     4741 actggctgga catggcgcag aggcgtatcc ggttccaggg cctgccggcg cgcatctgct
     4801 gggtcggtct cggcgaccgc caccggcttg gcctggcctt caacgagatg gtggctaagg
     4861 gtgaattgaa agcgcccatc gtcattggcc gcgatcatct cgattcgggt tccgtcgcct
     4921 ctccaaaccg cgagaccgaa gcgatgatgg acgggtccga cgccgtctcc gactggccgt
     4981 tgctcaacgc cctgctcaat tgcgcctccg gcgcgacctg ggtgtctctc caccatggcg
     5041 gcggcgtggg catgggcttt tcgcagcatt cgggcatggt gatctgctgc gatggtacgc
     5101 cggaggcggc gcggcggatc gtgcgggtgt tgtggaacga tcccgccacc ggggtcatgc
     5161 gccatgcgga cgccggctat gacatcgccc gcgactgcgc ccggacccat cggctcgacc
     5221 tgccgtcgct ggcctgatcg
//
LOCUS       DAOMXG010000169         5208 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_8472_length_5208_cov_10.439356, whole genome shotgun sequence.
ACCESSION   DAOMXG010000169 DAOMXG010000000
VERSION     DAOMXG010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 5208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5208
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8472_length_5208_cov_10.439356"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..953)
                     /locus_tag="PLQ11_10425"
     CDS             complement(108..953)
                     /locus_tag="PLQ11_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HRK25356.1"
                     /translation="MNTSEMGAMLRPFKHYQARHFRFEASDDGRIGHIRFNRPERKNP
                     LTFESYAELRDLFRDLVYASDIRAVVLSGEGGNFCSGGDVFEIIEPLTRMATPDLLAF
                     TRMTGDVVKAMRRCPQPVIAAVEGVCAGAGAILAMAADIRLATPEARTAFLFSRVGLA
                     GADMGACGILPRLIGQGRAAELLFTGRSMSAAEGEAWGFYNALHPAGVLAEKAEALAR
                     DLADGPWFAHGITKTMLNQEWAMGLEEMIESEAQAQALCMTTGDFRRAFEAFAARRKP
                     AFEGN"
     gene            complement(950..1726)
                     /locus_tag="PLQ11_10430"
     CDS             complement(950..1726)
                     /locus_tag="PLQ11_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRK25357.1"
                     /translation="MPQAEARHALITGAGSGIGAAIALRLAADGFRVTLAGRRSAPLE
                     AVAAAANGAVFVSAGFDVTDRASIADGLAAARAAFGPVSVLVNNAGEAPSATFAKTTP
                     EMWNHVLALDLGGVFNVTQAVLGDVRAAANGGRIVSVASTAGLKGYPYVTAYCAAKHG
                     VIGLTRALALELAGTGVTVNAVCPGFTETPLMEAALATVSARTGRPIEAIHAELVKAN
                     PQGRLIQPDEVADAVCWLVSTKAGSITGQSIAIAGGEVMQ"
     gene            complement(1761..4079)
                     /locus_tag="PLQ11_10435"
     CDS             complement(1761..4079)
                     /locus_tag="PLQ11_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003959 - NADPH dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="HRK25358.1"
                     /translation="MRIVCIGGGPAGLYFALLMKARHPAHHITVVERNRPYDTFGWGV
                     VFSDQVVDAITGADPRSAAEIADAFNHWDDIELKFKGRTIRTSGHGFIGIGRKHLLNI
                     LQRRCEELGVELVFEREVDSDLEFPDADLIIASDGIGSKIRERYRDEFAPDLLVRPNR
                     YIWFGTHKLYDAFTFDFQRSEHGWFQAHIYKFDGQTSTFIVETTEEAFLRHGLDKLDQ
                     EGSIAFCEKLFSDVLGGARLMTNARHLRGSAWLNFNRLICGRWSHFNGKSHVVLMGDA
                     AHTAHFAIGSGTKLAFDDAIELASQFDRIGHDRASIGAVLAAYEEERRIGVARVQNAA
                     RNAMEWFEVVGRRYADQLEPEQFMYSMLTRSQRISHENLRLRDAAYLDGFERWFAGRA
                     GVPARTDERPLPPAFTPYRLRSVTVPNRIVVSPMAMYSAIDGMPGDFHLAHLGARALG
                     GAGLVFAEMTCVSPDARITPGCLGLWNDAQAEGFRRIIDFVHSATAAKFAIQLGHAGR
                     KGATKRAWEGIDRPLESGAWPLLSASALPYLPDSVVPRAMSRADMDQVRADFVAATRR
                     AAAAGADWLEYHAAHGYLMSSFLSPLTNRRSDDYGGSHENRARFPLEVFRAIREVWPA
                     DRPISVRLSCHDWFEGGNLPEDAAIFARMFKEAGADMIDCSSGQVVKEEKPVYGRLFQ
                     TPFADLIRNEVGIPTIAVGAISDADQANSIIAAGRADLCAVARPHLADPAWTLHEAAR
                     LGLKIVPWPRQYISGKMQYETNLERAAAPATK"
     gene            4191..4730
                     /locus_tag="PLQ11_10440"
     CDS             4191..4730
                     /locus_tag="PLQ11_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004117125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRK25359.1"
                     /translation="MPEKTGQRPHSAADTPLGSFDDVEAIVRLEPRDQKRELRLWLRL
                     LTCANLIESEIRQRLRIDYDMTLPRFDLMAQLYREPDGLRLSDLSKRMMVSNGNLTAL
                     VDRLIADGYVSRTTDTNDRRAAVVQLTKAGTLIFSQLAEAHEGWLRALMGDLSPVQIQ
                     SLTQDLAQLKRSLIHRRQD"
BASE COUNT         1021 a         1701 c         1553 g          933 t
ORIGIN      
        1 caatgaatgc atccagatca gcgacatagg cgcggtgttc cggcccgaag aacggccaat
       61 cgagcgcagc gcgaccggga ccgttgaggg ggctttcggc cgccatgtca attgccttcg
      121 aacgcgggct tgcgccgggc cgcaaatgcc tcgaaggcac ggcggaaatc gcccgtcgtc
      181 atgcacaggg cctgcgcctg agcttcggat tcaatcatct cctccagacc catggcccat
      241 tcctgattga gcatggtctt ggtgatgcca tgggcgaacc agggcccatc ggctagatcg
      301 cgggccaggg cttccgcctt ttcggccagc accccggccg ggtgcagcgc attgtaaaag
      361 ccccaggcct ctccctccgc cgcgctcatc gaccggccgg tgaacagcag ctccgccgcc
      421 ctgccctgcc cgatcagccg cggcaggatc ccgcaggcgc ccatatcggc gccggcgagg
      481 ccgacgcggc tgaacaggaa cgcggttctc gcctccggtg tcgccagccg aatgtccgcc
      541 gccatggcca ggatcgcccc ggcgccggcg caaacgccct cgacggccgc gatgaccggc
      601 tgcggacagc ggcgcattgc cttgaccaca tccccggtca tccgggtgaa ggcgagcaga
      661 tcgggcgtgg ccatgcgcgt cagcggctcg atgatctcga acacatcgcc gcccgaacag
      721 aaattgcccc cctcgccgga aagcaccacg gcgcggatat cggaggcata aaccagatcg
      781 cggaacagat cgcgcagttc ggcatagctt tcaaaggtca gcggattctt gcgctccggc
      841 cggttgaacc ggatatggcc gatgcgcccg tcatcgctcg cctcgaaacg gaaatgccgc
      901 gcctgatagt gtttgaacgg gcgcagcatg gcgcccatct cgctggtgtt cattgcatga
      961 cctcaccgcc ggcaatggcg atcgactgac cggttatcga accggctttc gtggaaacaa
     1021 gccaacacac ggcatcggca acctcatcag gctggatcag ccggccttgc ggattggcct
     1081 tgaccaattc ggcgtgaatg gcctcgattg gccggcctgt cctggccgag accgtcgcca
     1141 gtgccgcctc catcagcggc gtttcggtaa agccggggca tacggcattg acggtgacgc
     1201 cggttccggc cagttccagc gccagcgccc gcgtcagccc gatgacgccg tgcttggccg
     1261 cgcaataggc cgtcacatag ggatagccct tgagccccgc tgtcgaggcg accgaaacga
     1321 tccggccacc attggccgcc gccctgacat caccaaggac ggcctgagtg acattgaata
     1381 cgccgccaag atcaagcgcc agcacatgat tccacatctc cggcgtggtt ttcgcaaagg
     1441 tcgcactcgg ggcctcgccg gcattgttga ccagcacgct gaccggaccg aaggccgcac
     1501 gcgccgccgc aagaccatcg gcgatcgacg cccggtcggt cacgtcgaat ccggcggaca
     1561 cgaacaccgc tccgtttgct gccgccgcga ccgcttcaag cggagcggag cgcctgccgg
     1621 ccagcgtgac cctgaaacca tccgcagcca gacgcagcgc gatcgctgcg ccgatgccgg
     1681 aaccggcgcc ggtgatcagg gcatggcgag cctcggcctg tggcattgcg gcattgtcct
     1741 ttctcctgca cgccggcggg ttattttgtc gccggcgcag cggcgcgttc gaggttggtc
     1801 tcgtattgca tcttgccaga gatgtattgc cgcggccagg gaacgatctt cagcccgagc
     1861 ctggccgcct catgcaacgt ccaggcggga tcggccagat gcggacgggc gacggcgcaa
     1921 agatcggcac gaccggcggc gatgatcgaa ttggcctgat cggcatcgga gatcgcgccg
     1981 accgcaatgg tcggtatgcc gacctcgttg cggatcaggt cggcaaacgg ggtctggaac
     2041 agccgcccgt agaccggctt ttcctccttg accacctgac cggaggagca atcgatcatg
     2101 tccgccccgg cctctttgaa catgcgggca aagattgcgg catcctccgg caggttgccg
     2161 ccctcgaacc agtcgtggca cgacaggcgg accgagatcg gccggtccgc cggccagacc
     2221 tcgcggatcg cccggaacac ttcgagcgga aagcgggcgc gattttcgtg gctgccgcca
     2281 taatcatcgc tgcgccggtt ggtcagcggc gacaggaagc tcgacatcag atagccgtgc
     2341 gcagcgtggt attccagcca gtcggccccg gctgcggcag cacggcgggt ggcggcgaca
     2401 aaatcggccc tgacctgatc catgtcggcg cggctcatcg cgcgcgggac cacgctgtcg
     2461 ggcagatagg gcagcgccga ggccgacagc agcggccagg ccccgctctc cagcggtcgg
     2521 tcgataccct cccaggcccg tttggttgcc cccttgcgcc cggcatggcc aagctggatg
     2581 gcgaatttgg ccgcagtggc ggaatgaaca aagtcgatga tccgcctgaa accctcagcc
     2641 tgggcgtcat tccacaggcc aagacagccg ggcgtgatcc gcgcatccgg cgaaacgcag
     2701 gtcatctctg caaaaaccag ccccgccccg ccaagcgccc gggcaccgag atgggccaga
     2761 tgaaagtcgc cgggcattcc gtcgatcgct gagtacatcg ccatcggcga gacgacgatg
     2821 cggttcggca ccgttacaga acgcagccgg tagggcgtga aggccggcgg cagcggtcgc
     2881 tcgtccgtgc gggcggggac acctgcccgg ccagcgaacc agcgctcgaa cccgtcaaga
     2941 taagccgcat cgcgcaggcg caggttctcg tggctgatgc gctgtgaccg ggtgagcatc
     3001 gaatacatga actgctcggg ttcaagctgg tcggcatagc gccggccgac gacctcaaac
     3061 cattccatgg cgttgcgcgc cgcattctgc acgcgggcaa cgccgatgcg ccgttcctcc
     3121 tcataggctg ccagcacagc cccgatcgag gcgcgatcat ggccgatgcg gtcgaactga
     3181 ctggccagtt cgatcgcatc gtcaaacgcc agcttggtgc ccgatccgat ggcaaaatgc
     3241 gccgtatgcg ccgcatcgcc catcagcacc acatgcgact tgccgttgaa atgcgaccac
     3301 cgcccgcaga tcagccggtt gaagttcagc caggccgagc cgcgcaggtg gcgggcattg
     3361 gtcatcagac gggcgccgcc cagcacatcg gaaaacagct tttcgcaaaa ggcgatcgag
     3421 ccttcctgat cgagcttgtc cagcccgtgc cgcagaaacg cctcctctgt cgtctcgacg
     3481 atgaaggtcg acgtctggcc gtcaaacttg tagatatgcg cctgaaacca gccatgctca
     3541 ctgcgctgga aatcgaaggt gaaggcatca tagagcttgt gcgtgccgaa ccagatgtag
     3601 cggttgggcc ggacaagcag gtcgggcgca aattcatccc ggtagcgctc gcggatcttc
     3661 gagccgatgc catccgaggc tatgatcaga tcggcgtcgg gaaactcgag atcgctgtcg
     3721 acctcccgct cgaaaaccag ttctacgccg agttcctcgc atcgcctctg caggatattg
     3781 agcaggtgct tgcgaccgat gccgatgaag ccatggccgc tggtgcggat cgtccgcccc
     3841 ttgaatttca gttcgatgtc atcccaatgg ttgaaggcat cggcgatctc ggcggcgctc
     3901 ctggggtccg cgccggtaat cgcatccacc acctgatcgg aaaacaccac accccagccg
     3961 aaggtgtcgt agggccggtt gcgctcgacc accgtgatat ggtgggccgg atgccgcgcc
     4021 ttcatcagca gcgcgaaata caacccggcc ggcccacctc cgatgcagac gatgcgcatg
     4081 ccattcctcc cgccgaatta ttttaagatt aaaatatgat gctcctgtgc tgtcaattgc
     4141 gtctttgtcc tgaatcgaaa ataatccgcc tccatggtcg aggaaacccg atgccagaaa
     4201 agaccggcca gcgaccgcac agcgccgcgg ataccccgct cggaagcttc gatgacgtcg
     4261 aggctatcgt ccggctggag ccgcgcgatc agaagcggga attgcgcctg tggctgcggc
     4321 ttctgacctg cgccaatctc atcgaaagcg aaatccgcca gcgtttgcgg atcgactatg
     4381 acatgacgct gccgcgtttc gatctgatgg cgcagctcta tcgcgagccg gacggcctgc
     4441 ggctcagcga tctgtccaaa cgaatgatgg tgtccaacgg caatctcacc gcccttgtgg
     4501 accggctgat cgccgatggt tatgtcagcc gcaccaccga caccaatgac cgacgtgcgg
     4561 ccgtcgtgca gctgaccaaa gccggaaccc ttatcttctc gcagcttgcc gaggcgcatg
     4621 agggctggct gcgcgccttg atgggcgatc tatcgcccgt ccagattcag tctttgaccc
     4681 aggacctcgc gcagctcaag cgctcgctga ttcaccgccg gcaagactag agcctgtcgc
     4741 gattaagtgg atgccggtta atcgcagaag acatactcta acctattgat accgatcacg
     4801 ttatccgcat tttgatgatt caatcaaaat gcggcgtgat ctagagcctg tcgcgattaa
     4861 gtggatgccg gttaatcgca gaagacatac tctaacctat tgataccgat cacgttatcc
     4921 gcattttgat gattcaatca aaatgcggcg tgatctagag tattttcagc aaattccaat
     4981 tcacaaagcg tgctctatct tttcattttt ccttattttt cttaacacga acaggactcc
     5041 acttcgcgtg aaaatgctcg aattacagca tgctccagcc ccaccaggcc cagaacactt
     5101 ggtcaaaaag ttcggaacgc tgcgtcgcgc tgctggatca cacctgccga ctgataccag
     5161 tggccctcac aaatgacctg tcggtcattt ctcgggccgt ccctcgca
//
LOCUS       DAOMXG010000170         5180 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_8535_length_5180_cov_10.056195, whole genome shotgun sequence.
ACCESSION   DAOMXG010000170 DAOMXG010000000
VERSION     DAOMXG010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 5180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5180
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8535_length_5180_cov_10.056195"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PLQ11_10445"
     CDS             complement(<1..295)
                     /locus_tag="PLQ11_10445"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HRK25360.1"
                     /translation="MRARLVALVCGVAMAMLALAEPLPVRALRDLLFDGYQRLAPRPF
                     DPAMPVRIIDIDDESLQRLGQWPWPRTILARLIATIGAGGPAALGLDIVLSEAD"
     gene            complement(300..1106)
                     /gene="xth"
                     /locus_tag="PLQ11_10450"
     CDS             complement(300..1106)
                     /gene="xth"
                     /locus_tag="PLQ11_10450"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HRK25361.1"
                     /translation="MELSVFCIATWNVNSVRQRIGLLCEWLRTRAPDVVCLQEIKCVD
                     EAFPTAEIEALGYNVAVHGQKTFNGVAILSKRPFDEVQRGLPGGDGDEQARYIEAVIS
                     HAAGAIRVASIYLPNGNPLGTEKFPYKLAWMERLRRHAAGLLAYEEPLVLAGDYNVIP
                     EARDARNPEQWLGDALFQPESRAALRALANLGLTDAIRATSDDPGLYTFWDYQAGAWQ
                     RDNGIRIDHLLLSPQAADRLIAAGIDRDERAREKPSDHVPVWVQLNLEAR"
     gene            1193..1948
                     /locus_tag="PLQ11_10455"
     CDS             1193..1948
                     /locus_tag="PLQ11_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25362.1"
                     /translation="MLNLNQFALPTVCERIATGGEMLNVGSALVIVLTALACLRHWIG
                     LSLRRDFAVVSLILMALAAGLGSALYHLDPGPTTLRLDTMPIQLFVMSSFAYILYRMI
                     GFDALVTLLNLAAFILVALLFGMMLPAALLGNGAHYVAPLIAIYILGAGLVIYARMGL
                     NHDAHLTGAAAARSDSVHIPRLKAGWGLLQVGIVFAMALVARSLDMPLCETLPYGTHF
                     IWHLLMAFATGRLLLVIIEFAASEQKALALASR"
     gene            2153..3325
                     /locus_tag="PLQ11_10460"
     CDS             2153..3325
                     /locus_tag="PLQ11_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HRK25363.1"
                     /translation="MLANALRPFNFDLGETADAIRETVRDFSVSEIAPRAEEIDRTNQ
                     FPRDLWPKMGALGLHGITVEEEFGGTGLGYLEHVIAMEEVSRASASVGLSYGAHSNLC
                     VNQIRRNGSLAQKRKYLPGLISGEHVGALAMSEPGAGSDVVSMKTRADKKGDRYILNG
                     NKMWITNGPIAETLVVYAKTDPNAGPRGITAFLIEKGMKGFSTAQKLDKLGMRGSDTG
                     ELVFADCEVPEENVLGSVGKGVNVLMSGLDYERAVLAAGPVGIMQACLDVVMPYVHER
                     KQFGQSIGEFQLVQGKIADMYVNMNSVRAYVYAVAKACDRGETTREDAAGAILIAAEK
                     ATQCALDAIQLLGGNGYINDYPTGRLLRDAKLYEIGAGTSEIRRMLIGRELFVKSM"
     gene            3406..4161
                     /locus_tag="PLQ11_10465"
     CDS             3406..4161
                     /locus_tag="PLQ11_10465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HRK25364.1"
                     /translation="MSDPVEGATRKLAEAMKAASAAAKLNDMMAPARALEKAMEPMRA
                     LERLADPFEAMRVASPQMEVVAAAQKATGAVGQVSAAAQAVANVNRAFDFSKGGLPAE
                     IQRTIDSMTTVSDKIRALAGYDLPRADIAKALGIRYQHVRNVLVRDEEKASESDAGGD
                     ESADKDAPAYVRLGPGGRVVIPAAIREALGLKENEQLLIEVTDGEVRLVPYAKVIRKV
                     QALVRKHVPEDVSLVDALLRERREETRQEHPDD"
     gene            4154..4537
                     /locus_tag="PLQ11_10470"
     CDS             4154..4537
                     /locus_tag="PLQ11_10470"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRK25365.1"
                     /translation="MIDAILDASALMALINDEPGALAVAEALPSSAIGVVNLAEACSV
                     MQMRGLSADVIETILGSLPLTAIDADQGLAVAAGLLRGRTSHRGLSLGDRFCLALAAR
                     EKLPVYTADRAWADLGLDLDIRLIR"
     gene            4598..>5180
                     /locus_tag="PLQ11_10475"
     CDS             4598..>5180
                     /locus_tag="PLQ11_10475"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRK25366.1"
                     /translation="MTNPFSFTLHARDGAARTGVITMPRGRIRTPVFMPVGTAGTVKG
                     LYPDQVRAAGADIILGNTYHLMLRPGAERVAALGGLHEFARWPHPILTDSGGFQVMSL
                     SGLRKLDRDGVTFKSHIDGSTHRLTPERSVEIQLLLDSDIIMQLDECIRLPAAPADVE
                     RAMLLSLDWAERCKHAFGAQPGRALFGIVQGGTL"
BASE COUNT          956 a         1606 c         1628 g          990 t
ORIGIN      
        1 ggtccgcctc cgacaggacg atatccagcc ccagcgcggc cggcccgccc gccccgatgg
       61 tggcaatcag ccgggccaga atcgtgcgcg gccacggcca ttggccgagc cgctgaaggc
      121 tttcatcatc aatgtcgatg atccggaccg gcatggcggg atcgaacggg cgcggcgcca
      181 gccgctggta gccatcgaac agaagatccc gcaaagcgcg gacgggcaat ggctcggcca
      241 gcgccagcat ggccattgcc acaccgcaga ccagtgcgac gagccgggcc cgcatcgcgc
      301 tatcgcgcct caaggttgag ctggacccag accggaacat gatctgacgg cttttcccgg
      361 gcgcgctcgt cgcgatcaat gccggcggcg atcagccggt cggcagcctg cggcgacagc
      421 aacagatgat caatgcggat gccgttgtcg cgctgccagg cgccggcctg atagtcccag
      481 aaggtataga ggcccggatc atcgctggtc gcgcggatcg catcggtcag gccgaggttg
      541 gccagcgcac gaagggctgc ccggctttcc ggctggaaca gcgcatcacc cagccattgc
      601 tcggggttcc tggcgtccct tgcctcggga atgacattgt agtcgccggc gagcaccaaa
      661 ggttcctcgt aagccagcaa cccggcggca tggcggcgca accgttccat ccacgccagt
      721 ttgtagggaa atttctcggt gccaagcggg ttgccgttgg gcagatagat cgaggcgacg
      781 cgaattgcgc cggcggcatg ggaaatcacc gcctcgatat agcgggcctg ctcgtcgccg
      841 tccccgccgg gcaagccgcg ctgcacctcg tcgaacggcc gtttggacag gatcgccacg
      901 ccgttgaagg ttttctgccc gtgcaccgcg acattgtagc ccaaagcctc gatctcggcg
      961 gtcggaaatg cctcatcgac gcatttgatt tcctgcaagc agacaacatc gggcgctctg
     1021 gttcgcagcc actcgcaaag cagcccaatg cgctggcgca cggaattcac gttccaggtt
     1081 gcaatacaga acacagaaag ctccatttcg ggcgccgcca tcaaaatgta tgcaacttcg
     1141 gccggcgcaa ggtcatagac tatttcaact gaaagcgatt cgggacgcgc cgatgctgaa
     1201 tttgaaccag tttgcactgc caactgtctg tgagcggatt gccaccggcg gcgaaatgct
     1261 gaacgtcggc tcggccttgg tgattgttct gaccgcgctg gcttgtttgc ggcattggat
     1321 tggcctgtcg ttacggcggg attttgcggt cgtatcgctg atcctgatgg ccttggcggc
     1381 gggtctgggc tcggcacttt accatctcga tccaggcccg acgaccctgc ggctggatac
     1441 aatgccgatc cagctgttcg tgatgtcgag ctttgcctac atcctctacc gtatgatcgg
     1501 gttcgacgcg ctggtgacgc tgctcaacct tgcggccttc attctcgtgg cgctgctgtt
     1561 tggcatgatg ctgccggctg cattgctcgg caacggcgcc cattatgtcg ccccgctgat
     1621 cgccatctat attctcggcg ccggactggt gatctacgcc cgcatgggcc tcaatcacga
     1681 cgcccatttg accggcgcgg ccgccgcacg ctcggattcc gtgcatattc cccgcctcaa
     1741 ggccggctgg ggcctgttgc aggtggggat cgtgttcgcc atggcccttg tggcgcgttc
     1801 gctcgacatg ccgctgtgcg agaccctgcc ctatggcacg catttcatct ggcatctgct
     1861 gatggccttc gccacgggac ggctgctgct ggtgatcatc gagtttgctg cctccgagca
     1921 gaaggcgctt gccctggcat cacgctgaga cgccgctgcg ggattcggca caaagcaggt
     1981 tgcctgccgg ctgccggctg gtaggctgga tcacgctccc gttttgatgg actcatcaaa
     2041 tcggggggtg acgtgatcgt tctcaagtgt ttagagcatg cttgtcgcga ttaaccggct
     2101 tccacttgat cgcagcatgc tctaacggcc taaacagaca actggattga tcatgctggc
     2161 gaacgctctc cgtcctttca attttgatct tggcgaaacc gccgatgcca tccgcgaaac
     2221 cgtgcgcgat ttctcggtga gcgaaatcgc cccgcgcgcc gaggagatcg accggacaaa
     2281 ccagtttccg cgtgatctct ggccgaagat gggcgcgctt ggcctgcatg gcatcaccgt
     2341 cgaggaggaa ttcggcggca ccgggcttgg ctatctcgaa catgtcatcg ccatggagga
     2401 ggtgagccgt gcctccgcct cggtcggcct cagctacggc gcccattcca acctctgcgt
     2461 caaccagatc cgccgcaatg gctcattggc gcagaagcgc aagtatctgc ccgggctgat
     2521 ctccggcgag cacgtcggcg cgctggcgat gtccgaaccc ggcgccggct cggacgtggt
     2581 gtcgatgaag acccgcgccg acaagaaggg cgaccgctat atcctcaacg gcaacaagat
     2641 gtggatcacc aacggcccca tcgccgagac cttggtggtc tacgccaaga ccgatccgaa
     2701 tgccgggccg cgcggcatca ccgccttcct gatcgaaaag ggcatgaagg gcttttccac
     2761 cgcccagaaa ctggacaagc tcggcatgcg cggctccgac accggcgaac tggtgttcgc
     2821 cgattgcgag gtgccggaag aaaatgtgct cggcagcgtc ggcaagggcg tcaacgtgct
     2881 gatgagcggg ctcgattacg aacgcgctgt gctggctgcc ggcccggtcg gcatcatgca
     2941 ggcatgtctt gatgtggtga tgccctacgt tcatgagcgc aagcagttcg gccagtcgat
     3001 cggcgaattc cagcttgttc agggcaagat cgccgacatg tatgtgaaca tgaattcggt
     3061 gcgggcctat gtctatgctg tggccaaggc ctgcgatcgc ggcgagacca cccgcgagga
     3121 cgcggccggc gccatcctga ttgccgccga aaaggcgacg caatgcgcgc tcgacgccat
     3181 ccagctgctc ggcggcaatg gctacatcaa cgattacccc accggccgcc tcttgcgcga
     3241 cgccaagctc tacgaaatcg gcgccggcac atccgaaatc cgtcgcatgc tgatcgggcg
     3301 ggaactgttt gtgaagagca tgtaacacaa aatatggcac aacatatgtg ttgttgacat
     3361 tataacctat atgacataga ttcgtggcaa tcgaaggagg ttgccatgag tgatcccgtt
     3421 gaaggggcga cgaggaagct ggccgaggcc atgaaagccg cgtcggcagc tgcgaagctc
     3481 aatgacatga tggcgccggc acgggcgctt gaaaaggcga tggagccgat gcgggccctg
     3541 gaacgcttgg ccgacccatt cgaagccatg cgcgttgcaa gtccccagat ggaagtcgtg
     3601 gctgccgcgc agaaggcaac gggcgctgtt ggccaggttt cggcagcggc gcaagctgtg
     3661 gccaacgtca atcgcgcgtt cgacttttcg aaaggtggcc tgcctgccga gatccagcgc
     3721 accatcgatt cgatgacgac agtatcggac aagatcagag cgcttgccgg ttatgatctt
     3781 ccgcgggcgg acatcgccaa ggcgctggga attcgctacc agcacgtgcg caacgtgctt
     3841 gtcagggatg aggaaaaggc atcggaatcg gatgcgggcg gagatgaaag cgctgacaag
     3901 gatgcgcctg cctatgtccg gctcggaccg ggtggccggg tcgtaatccc ggctgcgatc
     3961 cgcgaggctt tgggtctcaa ggaaaacgaa caactgctca tcgaggtcac cgacggcgaa
     4021 gtgcggcttg tgccctatgc aaaggtgata cgcaaggtgc aggcgctggt gcgaaagcac
     4081 gtcccggaag atgtcagtct tgtcgatgca ctcctgcggg agcgtcgtga ggaaacacgt
     4141 caggaacacc ctgatgattg atgcaattct ggatgcatcc gcgctgatgg ctctcatcaa
     4201 cgatgaaccc ggagccctgg cggttgccga agccctgcca tcatccgcaa taggcgtcgt
     4261 caatctggcc gaagcgtgca gtgtcatgca gatgcgcggg ctgtctgctg acgtcattga
     4321 aactattctt ggcagcctgc ccctgacggc catcgacgct gatcaggggc tggcagtcgc
     4381 tgctggtctg ctacgggggc ggacgagcca tcgtggcctt tccctcggcg accgcttctg
     4441 cctcgcgttg gccgcgcggg agaagcttcc tgtctacacg gcggaccggg cctgggctga
     4501 cctcggactc gatctcgaca tccgtctgat ccgctgactc gagccgcgat ggttgtcgcg
     4561 gtgcgacatc ggcgctaagt cgatttccgg acagattatg acaaacccgt tctccttcac
     4621 gctccacgcc cgcgacgggg cggcgcgcac cggtgtgatc accatgccgc gcgggcgcat
     4681 tcgcacgccg gtgttcatgc cggtcggcac ggccggaacg gtcaaggggc tttatcccga
     4741 tcaggtccgc gccgccggcg ccgacatcat tctgggcaac acctaccatc tgatgctgcg
     4801 ccccggcgcc gaacgggtgg ctgcgctcgg cggtctgcat gaatttgccc gctggccgca
     4861 tccgatcctc accgattccg gcggctttca ggtgatgtcg ctgtcgggcc tgcgcaagct
     4921 cgaccgcgac ggggtgacgt tcaagtcgca catcgacggc tcgacccacc ggctgacacc
     4981 agaacggtcg gtggaaatcc agcttctgct cgattccgac atcatcatgc aactcgacga
     5041 gtgcatccgc ctgccggccg cgccggccga tgtcgaacgg gcgatgctgc tgtcgcttga
     5101 ctgggccgag cgctgcaagc acgccttcgg cgcccagccg ggccgggcgc tgttcggcat
     5161 cgtgcaggga ggaaccctgc
//
LOCUS       DAOMXG010000171         5119 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_8680_length_5119_cov_11.029621, whole genome shotgun sequence.
ACCESSION   DAOMXG010000171 DAOMXG010000000
VERSION     DAOMXG010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 5119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5119
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8680_length_5119_cov_11.029621"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /locus_tag="PLQ11_10480"
     CDS             complement(<1..1004)
                     /locus_tag="PLQ11_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HRK25367.1"
                     /translation="MTPPLRPLKAGDHVFLVDGSSYIFRAYHALPPLTRKSDGLPVGA
                     VSGFCNMLWRLVRETVAGEKPTHLAVIFDHSSQTFRNQIYDQYKAQRPEPPEDLRPQF
                     GLVKEATRAFDIPAIEQEGFEADDLIATYARLAREAGATATIVASDKDLMQLVGAGVT
                     MFDTMKDRRIDVAEVIEKFGVPPGKVVEVQALSGDSVDNVPGVPGIGLKTAAQLITEY
                     GDLETLLARAGEIKQPKRRESLIEHAEKARISRRLVELDAHVPLVHPVEAIGVHQPDP
                     RRLVAFLKAMDFNTLTRRVGETFGLDPASIEPDARLTGAGAWMGKRGLPATDGEASAD
                     "
     gene            1095..2051
                     /locus_tag="PLQ11_10485"
     CDS             1095..2051
                     /locus_tag="PLQ11_10485"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRK25368.1"
                     /translation="MIVPARITRLDWMVRACIAAMAAFCFASPLLGGRPVVIQGEEFS
                     LAVLALMVAIAVLYGPRRRNLPGFARLAGLVADIMAQGLVFGMASYVFAGMAGPLMDG
                     RLAALDGAIGFDWLTTFRLVQSAPALEWALCIGYYSMMAQLGLWLLLLSWRGEHDRVR
                     LLINAFAINAILAIVLSGLVPAMGAFPYFGVLAADGLQSTAPVPGIVSGPYRELMALR
                     SGAMQEIVLVQTSGIVTFPSFHTMTGVVLAASVWHIGWLRLPALVLNALLIAATPPVG
                     GHYLVDTLAGLVLAALVLILCRHYVMTPLPAPAAAPAMAAQT"
     gene            2118..2783
                     /locus_tag="PLQ11_10490"
     CDS             2118..2783
                     /locus_tag="PLQ11_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRK25369.1"
                     /translation="MTAMISQPLAFVERSEEEIGERARTFRADMKRRRTVRDFTDRPV
                     PRAIIEDLVMTAAGAPSGANQQPWTFVCISDPAVKHRIRLAAEEEERAFYSGKAGEEW
                     LEALEHLGTDWEKPFLETAPWLIAIFARRWGHDAKGRKIKHYYVPESVGIATGFLIAA
                     VHNAGLASLTHTPAPMAFLNEICGRPDNEKPVILLVVGYPAEGCQVPVIDKKRFDEVA
                     AVL"
     gene            complement(2857..4536)
                     /gene="rpsA"
                     /locus_tag="PLQ11_10495"
     CDS             complement(2857..4536)
                     /gene="rpsA"
                     /locus_tag="PLQ11_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HRK25370.1"
                     /translation="MSASNREDFAALLEESFARTDMAEGSVIKGRVVAIEKDDAVIDV
                     GLKTEGRVKLKEFAGPGKDATIKVGDEVEVYLERVENAMGEAVISRDKARREESWVKL
                     EQAFEKQEKVNGIIFNQVKGGYTVDLEGAVAFLPRSQVDIRPIRDVTPLMGVSQPFQI
                     LKMDRRRGNIVVSRRTVLEETRAEARSELVANLEEGQTVDGVVKNITDYGAFVDLGGI
                     DGLLHVTDIAWRRVNHPTEVLTVGQTVKVKIIKINQDTHRISLGMKQLMGDPWASIEA
                     KYPIEAKFTGRVTNITDYGAFVELEPGIEGLIHVSEMSWTKKNVHPGKILSTSQEVEV
                     QILEVDSTKRRISLGLKQTLANPWEAFISRHPVGSVVEGEVKNKTEFGLFIGLYGDVD
                     GMVHLSDLDWNRPGEQVMEEFKKGDVVKAVVLDVDVEKERISLGVKQVASDPFADAGE
                     IKKGQIVTCEVLEVKDSGLEVKLVGTDLTSFIKRSEIARDRGEQRPERFAAGEKVDAR
                     VTLFDRKARKVQVSIKALEVAEEKEAMAQYGSADSGATLGDILGAALKRGK"
     gene            complement(4765..>5119)
                     /locus_tag="PLQ11_10500"
     CDS             complement(4765..>5119)
                     /locus_tag="PLQ11_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019994584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HRK25371.1"
                     /translation="PDVRTALREYQQRFAAQPGGAVLDGRDIGTVIAPQAEVKLWVTA
                     TPEVRARRRFLELKPKRPDLEEAVILGELLERDGRDAPNMARARDAHLLDTSVMDIEQ
                     AFQAAVAIVEAVRKR"
BASE COUNT          818 a         1630 c         1655 g         1016 t
ORIGIN      
        1 aaatcggcgc tggcttcgcc gtcggtggcg ggcaaccccc gcttgcccat ccaggctccg
       61 gcgccggtca gccgcgcatc cggctcgatg gaggccggat caaggccgaa agtctcgccg
      121 acgcgccgcg tcagcgtgtt gaaatccatc gccttgagga aggccaccag ccggcgcggg
      181 tcgggctgat gcacgccgat cgcctcgaca ggatggacga gcggcacatg cgcatcgagt
      241 tcgacgaggc gccgcgagat gcgggctttc tctgcatgct cgatcagcga ctcgcggcgc
      301 ttgggctgct tgatttcgcc ggcgcgggcg agcagcgttt cgagatcgcc atattcggtg
      361 atgagttgcg cggcggtttt cagcccgatg ccggggacac ccggcacatt atcgaccgaa
      421 tcgcccgaca gtgcctgcac ttcgacgact ttccccggtg gcacgccgaa tttctcgatg
      481 acctcggcga cgtcgatgcg ccggtccttc atcgtgtcga acatggtcac gcccgcaccg
      541 accagttgca tcaggtcctt gtcggaggcg acgatggtgg cggtggcgcc ggcctcgcgt
      601 gccagtcgcg cataggtggc gatcagatcg tccgcctcga acccttcctg ctcgatggcc
      661 ggaatgtcga aggcgcgcgt tgcctccttc accaggccga actgcgggcg cagatcctcc
      721 ggcggttcgg gccgctgcgc cttgtattga tcgtagatct ggttgcggaa cgtctgcgag
      781 gaatggtcga agatgacggc aagatgcgtg ggcttttcgc ccgccacggt ctcgcgcacc
      841 agccgccaca gcatgttgca gaagccggag acggcgccca ccggcaggcc gtcgctcttg
      901 cgcgtcagcg gcggcagggc gtgataggcg cggaagatgt aggacgagcc gtcgaccagg
      961 aacacatggt cgccggcttt gagcgggcgc agcggaggtg tcatgccggc gactataggc
     1021 cggccctcat gcaccccgca aacacaacgt taaagcccgc gtgcgacaag gggagtgcta
     1081 tcggatctaa cgtcatgatt gttcccgccc gaatcacccg ccttgactgg atggtgcgcg
     1141 cctgcatagc ggcgatggcg gcgttctgct ttgcctcgcc gcttctgggt ggacgtccgg
     1201 tcgtcatcca gggcgaggaa ttcagccttg cggttctggc gctgatggtt gccatcgctg
     1261 tgctttacgg cccccgccgc cgcaatcttc cgggctttgc gcgccttgcc ggccttgttg
     1321 ccgatatcat ggcgcagggg ctggtgttcg gcatggcttc ctatgtcttt gccggcatgg
     1381 ccggcccgct gatggatggc cggctggccg cactggatgg agcgatcggc tttgactggc
     1441 tgacaacctt tcgcttggtc cagtctgcgc cggcgctcga atgggcgttg tgcatcggct
     1501 attattcgat gatggcgcag cttggcctct ggctgctgct gctgtcctgg cgcggtgagc
     1561 atgatcgggt ccggctgctg atcaatgctt ttgcgatcaa cgccatcctt gcaattgtcc
     1621 tgtccggtct tgtcccggcg atgggggcct ttccgtattt tggcgtgctt gcggccgatg
     1681 gcctccagtc aacggcgccg gtgccgggca tcgtctcggg gccctaccgc gaactgatgg
     1741 cgctgcgctc cggcgcgatg caggagatcg tgctggtcca gaccagcggg atcgtgacat
     1801 ttcccagctt tcacacgatg accggcgtgg tgctggctgc ctccgtctgg cacatcggct
     1861 ggctgcgcct gcctgcgctt gtgctcaacg ctctgctgat cgctgcgacg ccgccggtgg
     1921 gcgggcatta tctggtcgat acgctcgccg ggttggtgct ggccgctttg gtgctcatcc
     1981 tctgtcgcca ctatgtgatg acgccgttgc cggctcctgc ggccgctccc gcgatggcag
     2041 cgcagacctg acggacttgg caagtccggg cttccggtta cagattgctg ttttgcgccg
     2101 atggagttcg acggtcgatg acagcaatga tcagccagcc gctggcattt gtggaacgca
     2161 gcgaggagga gatcggcgag cgggcccgca ccttccgtgc cgacatgaag cgtcgccgca
     2221 cggtgcgcga ttttaccgat cggccggtgc cgcgcgccat catcgaggat ctggtgatga
     2281 cggcagcggg cgcaccgtcc ggcgccaacc agcagccatg gaccttcgtc tgcatctccg
     2341 atcctgccgt caagcacagg atccgccttg ccgccgagga ggaggaacgg gccttttata
     2401 gcggcaaggc tggcgaggaa tggctggaag cgctggaaca tcttggcaca gactgggaaa
     2461 agccgtttct cgaaaccgcg ccgtggctga tcgccatttt cgcccggcgc tggggccatg
     2521 atgcgaaagg ccgcaagatc aagcattact acgtgccgga aagcgtcggc atcgccaccg
     2581 gcttcctgat cgcggcggtg cataacgccg gccttgccag tctcacccat acgccggcac
     2641 cgatggcctt cctcaacgaa atctgcggcc ggccggacaa tgaaaagccg gtgatcctgc
     2701 tcgtcgtcgg ctacccggca gagggctgcc aggttccggt gatcgacaag aagcgtttcg
     2761 acgaggttgc cgctgtcttg taagcgacat cggtgccaaa cgaaaaaccc cggcgtttcc
     2821 gccggggtct tttcgttctc aagtctttga aaagacttac ttgccgcgct tgagggcggc
     2881 gccgagaata tcgccgagcg tagccccgga atcggccgag ccgtattggg ccatggcttc
     2941 cttctcctcg gccacttcca gcgccttgat cgacacctgc accttgcgcg ccttgcggtc
     3001 gaacagggtg acacgggcat cgaccttctc gccggcggcg aagcgctcgg ggcgctgctc
     3061 gccacggtca cgggcgattt ccgaccgctt gatgaacgag gtcaggtcgg tgccgaccag
     3121 cttgacctcg aggccggaat ccttgacctc gagcacctcg caggtgacga tctggccctt
     3181 cttgatctcg ccggcatcgg cgaacgggtc ggaggcgacc tgcttgacgc cgaggctgat
     3241 gcgctccttc tcgacatcga cgtcgagaac gaccgccttg accacgtcgc ccttcttgaa
     3301 ctcctccatc acctgctcgc ccggacggtt ccagtcgaga tcggagaggt ggaccatgcc
     3361 atcgacatcg ccatagaggc cgatgaacag gccgaactcg gtcttgttct tgacctcgcc
     3421 ctcgaccaca gagcccaccg gatgacggga aatgaaggct tcccacggat tggccagcgt
     3481 ctgcttgagc ccgagcgaga tgcggcgctt ggtcgaatcg acctcgagga tctgcacttc
     3541 cacttcctgc gaggtggaga ggatcttgcc ggggtggacg ttcttcttgg tccaggacat
     3601 ttccgagacg tggatcagac cctcgatgcc cggctccagc tcgacgaacg cgccgtaatc
     3661 cgtgatgttg gtgacgcgac cggtgaactt ggcctcgatc ggatacttgg cctcgatgct
     3721 ggcccaggga tcgcccatca gctgcttcat gccgaggctg atgcggtggg tgtcctgatt
     3781 gatcttgatg atcttgacct tgacggtctg gccgacggtc agcacctcgg tcgggtggtt
     3841 gacgcggcgc caggcgatgt cggtcacgtg caggaggccg tcgatgccgc ccagatcaac
     3901 gaacgcaccg taatcggtga tgttcttgac gacgccgtcg accgtctggc cttcctcgag
     3961 gttggccacc agttccgacc gggcctcggc gcgggtctct tccagcacgg tgcggcgcga
     4021 gacgacgata ttgccgcgcc ggcgatccat cttgaggatc tggaacggct gggagacgcc
     4081 catcagcggg gtgacgtcgc ggatcggacg gatgtccacc tgcgaacgcg gcaggaacgc
     4141 cacggcgcct tccagatcga cggtgtagcc gcccttgacc tgattgaaga tgatgccgtt
     4201 gaccttctcc tgcttctcga acgcctgttc gagcttgacc caggattcct cgcggcgggc
     4261 cttgtcacgc gagatgacgg cctcgcccat cgcgttctcg acacgctcga gatagacctc
     4321 gacctcgtcg ccgaccttga tggtggcatc cttgcctggt ccggcaaatt ccttcagctt
     4381 gacgcggccc tcggtcttga ggccgacatc gatcaccgcg tcatctttct cgatggcgac
     4441 gaccctgccc ttgatgaccg agccttcggc catgtcggtg cgcgcaaacg attcttcgag
     4501 cagcgcggcg aaatcttcac ggttggaagc ggacatttat gctccggacg aaggcgtggc
     4561 cagggcctcc gatcatacgt tcgtcggcag acgagggttg ggtgaacccg ttcgaccgcc
     4621 ggccgcctcc aggcctggag acgtgaacag gcggggcctg tcctgccgac atgttgaacc
     4681 agcggatcgc gggtttggtg tctcaaatac cgccggctcg gaagccgccg gacaatgcga
     4741 aaaagggcgc ggcagcccgc accctcaccg tttccggacg gcttcgacga ttgccacggc
     4801 cgcctggaag gcctgctcta tatccataac ggaggtatcg agcaagtgcg catctctcgc
     4861 acgggccatg ttcggcgcat cgcggccgtc gcgctcgagc aattcgccga gaatgaccgc
     4921 ttcctcgagg tccggtcgct tcggcttgag ttcgagaaag cggcgccggg cgcgcacctc
     4981 gggcgttgcc gtcacccaca gcttcacctc cgcctgcggg gcgatcaccg tgccgatgtc
     5041 gcggccgtcg agcacggctc cgccgggctg ggcggcgaag cgctgctgat attcgcgcaa
     5101 ggcggtgcgc acatcgggc
//
LOCUS       DAOMXG010000172         5082 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_8764_length_5082_cov_9.099264, whole genome shotgun sequence.
ACCESSION   DAOMXG010000172 DAOMXG010000000
VERSION     DAOMXG010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 5082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5082
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8764_length_5082_cov_9.099264"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1061)
                     /gene="murG"
                     /locus_tag="PLQ11_10505"
     CDS             complement(<1..1061)
                     /gene="murG"
                     /locus_tag="PLQ11_10505"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HRK25372.1"
                     /translation="MTGPLRVVLAAGGTGGHLFPAEALANVLIARGAEVDLVSDDRAA
                     AFAASFPAKLHVVKAGTVTGSGLVAKIRGALALLGGTRQALGLMSRLRPDVVVGFGGY
                     PTVPPVYAATIRGIPTIIHEANGVMGRANRLLSSRVTRIATGVPLKDLRFPDKTVVTG
                     SPVRPPVLAAAEVPFASPAQGGRLGLMVFGGSQGARVMGEVVPPALAMLPELLRGRID
                     LVQQVRSEDLDKVANAYRLMGVTADLAPFFKDLPARMAGAHLVISRSGASTVAELAVI
                     GRPSILVPLPGSIDQDQATNARQLAEAGAALVCPQADFTPEWLAGEIARMMAEPRALA
                     DMAARARALAAGGRPDVRS"
     gene            complement(1058..2215)
                     /gene="ftsW"
                     /locus_tag="PLQ11_10510"
     CDS             complement(1058..2215)
                     /gene="ftsW"
                     /locus_tag="PLQ11_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HRK25373.1"
                     /translation="MVSRAERSLVNEWWWTIDRWLLFSFFALMLLGILFGLAASPPVA
                     NRLGLSTFHFVNRQALFLVLAAAVMVGVSFLDLRHIRRLALVVFVVALGLVIATLIFG
                     AEVKGARRWISLAGISIQPSEFLKPAFVMLAAWAFSENAGRQDMPGTMIGFALLPMVV
                     VPLILQPDFGQTMLVSAVWCGLFFLAGLHLLWVVGLGGAGAVGLLLAYKFLPHVTSRV
                     DKFLNKGSGGQGEFQAEMARESMLSGGLFGKGPGEGTVKRVLPDSHTDYIFAVIGEEF
                     GLIVCLVVVALFALIVLRALTIAFRNQEPFARFAAAGLAMLFGLQASINIAVNLDLIP
                     PKGMTLPFISYGGSSLVSLALTTGFLLAVTRRRPTASVIHRVPPGLAAVPA"
     gene            complement(2218..3615)
                     /gene="murD"
                     /locus_tag="PLQ11_10515"
     CDS             complement(2218..3615)
                     /gene="murD"
                     /locus_tag="PLQ11_10515"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002964541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HRK25374.1"
                     /translation="MTPVATFKGKRIALFGLGGSGLVTAEALVAGGAKVHAWDDSEAA
                     RAEAERRGISLKDLGKTAFDAYEALVLAPGVPLTHPEPHWSVNKARDAGIEIIGDVEL
                     FCRERTLLAPFAPFVAITGTNGKSTTTALIAHLLKTAGRGVQLGGNIGTAVLALEPPA
                     EGRIHVIEVSSFQIDLAPSLVPSIGVMMNLTPDHIDRHGTVENYAAIKERMIAAADLA
                     VIGIDDDFSRAMAERREAAGKAVVRVATRAVLNRGLFVDGTKVIFAHASKTIEAADLA
                     GLGALRGRHNAQNAAMAIAVATSLGVSGEAIREGLRTFGGLAHRMEEIGQVGRVLFIN
                     DTKATNADSTEKALASFERNIYWIVGGKPKEGGIEPLRPYFDRIAKAYLIGEAADAFA
                     ATLGEAVRHVHAGTLDVAVEMAMSDAGRDNDAVVLLSPACASYDQYKNYEQRGNHFRV
                     LVEALNGFVPKQKGA"
     gene            complement(3620..4702)
                     /gene="mraY"
                     /locus_tag="PLQ11_10520"
     CDS             complement(3620..4702)
                     /gene="mraY"
                     /locus_tag="PLQ11_10520"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HRK25375.1"
                     /translation="MLFWLSDFSQVFGPLNLFRYITFRAGGATVTALFFVLMFGPAII
                     NLLRIKQGKGQPIRDDGPETHFAKKGTPTMGGLMILSGLLVACLLWCNLSNPFVWAVL
                     GVTVGFGAIGFYDDYLKVTKQSHKGFSGKARLTLEAIIAGIAVYLMTQNFPPQLAQVV
                     SLPVLKDAALHLGVAFVVFGIFIIVAAGNAVNLTDGLDGLAIVPVMIAAGTFGFIAYL
                     AGNAIFANYLQINFIPRVGELAVILGAVIGAGLGFLWFNAPPAQIFMGDTGSLALGGL
                     LGSVAVATKHEIVFAIVGGLFVLEAMSVIIQVASFKLTGKRVFRMAPIHHHFEKKGWQ
                     EPQVVIRFWIIAVILALIGLATLKLR"
     gene            complement(4710..>5082)
                     /locus_tag="PLQ11_10525"
     CDS             complement(4710..>5082)
                     /locus_tag="PLQ11_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012319329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamyl-2,
                     6-diaminopimelate--D-alanyl-D-alanine ligase"
                     /protein_id="HRK25376.1"
                     /translation="LAVLASLRPEPGGRRIAILGDMLELGAAGAELHAGLAAAVVDSG
                     TDCVFAAGPLMKNLVDALPKARVGGYAADSTALEPLVLAAVRAGDVVTVKGSNGSRMA
                     RIVAALKARYPAMAGPGAEAK"
BASE COUNT          995 a         1793 c         1533 g          761 t
ORIGIN      
        1 tgtgatcgaa cgtcggggcg accgcctgct gccagcgccc tggccctggc agccatgtcg
       61 gcgagcgcgc gcggttcggc catcatccgg gcgatttcgc ccgccagcca ttccggcgtg
      121 aagtcggcct gcggacacac cagcgccgca cctgcctccg caagctggcg cgcattggtt
      181 gcctgatcct gatcgatgct gcccggcagc ggcaccagga tcgacggtct gccgatcacc
      241 gccagttccg ccacggtcga ggcgccggag cggctgatga ccagatgcgc acccgccatg
      301 cgcgccggca gatccttgaa gaatggcgca agatcggcgg tgacacccat cagccgatag
      361 gcattggcga ccttgtcgag atcttccgaa cgcacctgct ggacaagatc gatgcgtccg
      421 cgcagcagct ccggcagcat agccagcgcc ggcggcacca cctcgcccat cacccgcgcg
      481 ccctggcttc caccgaacac catcagcccc agccgaccac cttgcgccgg cgacgcgaat
      541 ggcacctccg ccgccgcaag aaccggcggc cgcaccggac tgccggtcac tactgtcttg
      601 tccggaaagc gcaggtcttt cagcggcaca ccggtggcga tgcgcgtgac acgcgaggac
      661 aacaaccggt tggcgcggcc catcacgccg tttgcctcat ggatgatcgt cggaatcccg
      721 cggatggtcg ccgcatagac cggcggcacg gtgggatagc cgccaaagcc gacgacgaca
      781 tcgggcctga gccgactcat caggcccaga gcctgacggg ttccgccgag cagcgccaac
      841 gcgccccgga tcttggcgac gaggccactg ccagtcaccg tgccggcctt gacgacatgc
      901 aacttcgccg gaaagctcgc cgcaaaggca gcggcgcggt catcgctgac aagatcgacc
      961 tcggcgccgc gcgcgatcag cacattggcc agcgcctcgg ccggaaacag atgtcccccc
     1021 gtccccccgg ccgccagcac gaccctgagc ggccctgtca tgccggcacc gccgcgagcc
     1081 cgggtggcac gcgatggatc accgaggcgg tcggccgccg ccgcgtgacc gccagcagga
     1141 atccggtcgt cagcgccagc gagaccagcg acgaaccgcc gtaggaaatg aacggcagcg
     1201 tcatcccctt cggcgggatg aggtcgagat tgacggctat gttgatcgac gcctgcaagc
     1261 cgaacagcat cgccagcccg gcagcggcaa agcgcgcgaa cggttcctga ttgcggaatg
     1321 cgatggtcag ggcccgcagg acgatcagcg cgaacagtgc caccaccacc aggcacacga
     1381 tcagtccgaa ttcctcgccg atcaccgcaa agatgtaatc ggtatggctg tcgggcagca
     1441 cgcgcttgac cgtgccctcg cccggtccct tgccgaacag cccgcccgac agcatgcttt
     1501 cgcgtgccat ctcggcctga aactctccct gtccgccgct ccccttgttg aggaacttgt
     1561 cgacgcgcga ggtcacatgc ggcaggaact tgtaggccag cagcaggccg actgcaccgg
     1621 cgccgccgag accgacgacc cacagaagat gcagcccggc cagaaagaaa agcccacacc
     1681 ataccgccga aaccagcatg gtctggccga agtccggctg aaggatcagc ggcaccacga
     1741 ccatcggcag cagcgcaaac ccgatcatcg ttcccggcat gtcctgccgg ccagcattct
     1801 cggaaaacgc ccaggccgcc agcatgacaa aggccggctt gaggaactcg gacggctgga
     1861 tcgagatgcc ggcgagcgaa atccagcgcc tggccccctt cacctcggcg ccgaagatca
     1921 gcgttgcgat gaccagcccc agcgccacaa caaacaccac cagcgccagc cgtcggatat
     1981 gtctgaggtc gaggaacgaa acccccacca tcaccgcagc cgccagaacc aggaacaatg
     2041 cctgccggtt gacgaaatgg aaggtggaca gccccagccg gttggcgacc ggcgggctgg
     2101 cggccagccc gaacagaatg ccgagcagca tcagcgcgaa gaaactgaac aagagccagc
     2161 ggtcgatggt ccaccaccat tcgttgacca gcgagcgttc ggcgcggctg accatggtca
     2221 ggctcccttc tgtttcggaa cgaacccgtt gagcgcttcg accagcacgc ggaaatgatt
     2281 cccgcgctgt tcgtagttct tgtattggtc gtaggacgcg caggccggtg acagcagcac
     2341 cacggcgtca ttgtcgcgcc cggcatcgga catcgccatt tcaaccgcga catccagcgt
     2401 cccggcatgg acatggcgca ccgcctcccc cagcgtcgcc gcaaaggcgt ctgcggcttc
     2461 accgatcaga taggctttgg cgatgcggtc gaaatacggc ctgagcggct cgatcccgcc
     2521 ttccttgggc ttgccgccga caatccagta gatattgcgt tcgaacgagg ccagcgcctt
     2581 ctcggtcgaa tcggcattgg tcgccttggt gtcgttgatg aacagcacgc ggccaacctg
     2641 gccgatctcc tccatgcgat gggcaaggcc gccgaatgtc cgcaatccct cgcggatcgc
     2701 ctcgccgctg accccgagcg aggtcgccac cgcgatagcc atggccgcat tctgggcgtt
     2761 gtgccggccg cgcaaggcgc ccagaccggc cagatctgcc gcctcgatgg tcttgctggc
     2821 atgggcgaag atgacctttg tgccatcgac gaaaaggcca cggttcagca cggcgcgggt
     2881 cgccacccgc accaccgcct tgccggcggc ttcccgccgc tcggccatgg cgcggctgaa
     2941 atcatcgtcg atcccgatca ccgcaaggtc ggcagcggcg atcatgcgtt ccttgatcgc
     3001 ggcgtaattc tccaccgtgc catgccggtc gatgtggtcc ggcgtcagat tcatcatcac
     3061 cccgatgctc ggcaccagcg acggggcaag gtctatctgg aacgacgaga cctcgatcac
     3121 atggatacga ccttccgccg gcggctccag cgccagcacg gcggtcccga tattgccgcc
     3181 gagctgcaca ccacgcccgg cggttttcag gaggtgggcg atcagcgccg tcgtcgtcga
     3241 tttgccattg gtaccggtga tcgcgacaaa cggtgcgaac ggcgccagca gcgtccgctc
     3301 ccggcagaac agttcaacgt cgccgatgat ctcgatgccc gcatcgcgcg ccttgttcac
     3361 cgaccagtgc ggctcgggat gggtgagcgg cacgccgggc gccaggacca gcgcctcata
     3421 ggcgtcgaac gcggtcttgc cgaggtcttt cagcgaaatt ccgcgccgct ctgcctcggc
     3481 gcgcgccgcc tcgctgtcat cccaggcgtg aaccttcgcc ccgcccgcga ccagcgcctc
     3541 ggcggtgacc agccccgagc cacccagccc gaacagcgca atcctcttgc ccttgaacgt
     3601 cgcaaccggt gtcatcggct cacctcaact tcagtgtggc gagaccgatc agcgccagaa
     3661 tgacggcgat gatccagaag cggatgacga cctgcggctc ctgccatccc ttcttctcga
     3721 aatgatggtg gatcggcgcc atgcggaaca cccgcttgcc ggtcagcttg aacgacgcca
     3781 cctgaatgat cacgctcatt gcctccagca cgaacagccc gccgacgatg gcaaagacga
     3841 tttcatgctt ggtcgccacc gccaccgagc ccagcagccc gccgagcgcg aggctgccgg
     3901 tatcgcccat gaagatctgc gccggcggcg cgttgaacca caggaagccg aggccagccc
     3961 cgatgaccgc ccccagaatg acggccagtt cgccgacgcg cgggatgaag ttgatctgca
     4021 ggtaattggc aaaaatcgca ttgccggcga gataggcgat gaacccgaac gttccggcag
     4081 cgatcatcac cggcacgatg gccagcccgt caagaccatc ggtgaggttc accgcgttgc
     4141 ccgccgccac gatgatgaaa atgccgaaaa cgacaaaggc cacgccgaga tgcagcgccg
     4201 catccttgag caccggtagg ctgacgacct gggcgagctg cggcggaaaa ttctgcgtca
     4261 tcagatagac ggcgatgcca gcgatgatcg cttccagcgt cagccgcgcc ttgccggaaa
     4321 agcccttgtg gctctgcttg gtcaccttca gataatcgtc atagaagccg atggcgccga
     4381 atccgaccgt gacccccagc accgcccaga cgaacggatt ggaaaggttg caccacaaca
     4441 gacaggcaac cagcaggccc gacaggatca tcagcccgcc catcgtcggg gtgcccttct
     4501 tggcaaaatg cgtttccggc ccgtcgtcac ggatcggttg gcccttgccc tgcttgatcc
     4561 tgagcaggtt gataatcgcc ggcccgaaca tcagcacgaa gaacagcgcc gtcactgtcg
     4621 ccccgcccgc ccggaaggtg atgtagcgga acaggttgag cggtccgaaa acctggctga
     4681 aatcggaaag ccagaacagc atgaaagcct cacttcgctt cggcgccggg cccggccatc
     4741 gccggatacc gcgccttgag ggcggcaacg atccgcgcca tgcgcgagcc gttcgagccc
     4801 ttgacagtca ccacatcgcc ggccctcaca gccgcaagca caagcggttc gagcgcagtc
     4861 gaatcggctg cgtagccccc aacccgcgcc ttcggcaagg catcgacgag gtttttcatc
     4921 aagggaccgg ccgcaaacac gcaatcggtg ccggagtcca cgacagcggc agcaagaccg
     4981 gcatgcagct ccgcccccgc agcgccgagt tcgagcatgt cgccgaggat ggcgattcgc
     5041 cgcccgcccg gctccggccg caggctcgcc agcaccgcca gt
//
LOCUS       DAOMXG010000173         4991 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_8977_length_4991_cov_9.805105, whole genome shotgun sequence.
ACCESSION   DAOMXG010000173 DAOMXG010000000
VERSION     DAOMXG010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4991
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8977_length_4991_cov_9.805105"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="PLQ11_10530"
     CDS             <1..351
                     /locus_tag="PLQ11_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRK25377.1"
                     /translation="TDLRAAHQRQARLTNEALNARGLPVMENPSHIVPVLVGDAERCK
                     AASDRLMERHGIYIQPINYPTVPRGTERLRITPTPLHTDAHIARLAGALDEVWSALGL
                     QRRRQGADTLIAAE"
     gene            358..1068
                     /locus_tag="PLQ11_10535"
     CDS             358..1068
                     /locus_tag="PLQ11_10535"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRK25378.1"
                     /translation="MPNPSLTPMPATQEDHWTIRHSSLFGRLPDTTFRTLFEGKPVHV
                     AGKAMPLFAWGTPARTCFIVLKGLVKLFRTSETGEAAVLSICGPCRPLMLAEGLTAKP
                     YSASAETVSPVRIMCLDVAGLRQSMAENSKLSMALLAAAASDLRSLVAHVEELKAMTG
                     PARLAAMILNLSEARAGAMQITLPYEKQLIAGRLGMTPESFSRAMRQLKDQGVKVSRD
                     RLVITDIERLRGFLNTRV"
     gene            complement(1081..1815)
                     /locus_tag="PLQ11_10540"
     CDS             complement(1081..1815)
                     /locus_tag="PLQ11_10540"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRK25379.1"
                     /translation="MKLETDSRPAGIALDSRDWQTVRSSALFAEVDHRVLEGLCGHQH
                     PISYAPRQLIFAQGDQAEGFYLVLDGWVKLFRLSPSGDEAIVGVFTRGESFAEAVFFL
                     GGTYPASAEAASTLRLLRIDAGRFNEAIEAEPGLAATLLGSVVQHTERLFAEIASLKL
                     MSTQRRLADFLVQQTVPGAATATVVLPYEKALLAARLGMTPESLSRALASLRKLGVTV
                     MRDQVLMADVAGLAAYARPGKTARQG"
     gene            1885..2304
                     /locus_tag="PLQ11_10545"
     CDS             1885..2304
                     /locus_tag="PLQ11_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25380.1"
                     /translation="MRGPIGAMVVVAASAWPLACLAQRDGVADALAGHQAEMNQLAGK
                     DPMRRAYFFDPVAVEGPDPGVPCDHIAQRRPEAVAGPGKVVLLESVAEAGRMLRFWLP
                     PGAEGSSDPVAAAADYAGESGFGPYRLIGVRQFSWCR"
     gene            2397..4055
                     /locus_tag="PLQ11_10550"
     CDS             2397..4055
                     /locus_tag="PLQ11_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006938174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HRK25381.1"
                     /translation="MTLKTQGAKMLGIALGAFLSTTAFTPLYAQQAAPAAATPTLSKE
                     AMEASGNIYFQRCAGCHGVLRKGATGKNLEPQNSAKLGQERLEKIIALGTDGGMVNFD
                     DILTKDEIKNIATYIQMPPQSPPEWGMKEMMASWKNIVPPDQRPKQQMNKINLKNVFS
                     VTLRDAGEVALIDGDTKQIISRVKTGYAVHISRLSASGRYVYVIGRDGLLSLIDLWME
                     KPAVVAEMKSGLDARSVDVSKFKGFEDKYAIAGSYWPPQYVIMEGDTLKPLKVVSTRG
                     VTVDGEYHPEPRVASIVASMIKPEWVVNIKESGLIQLVDYSDIKNLKTTTIESAKFLH
                     DGGWDASKRYFLVAANASNKVAVVDTKEGKLAALVDTAKIPHPGRGANFTHPKFGPVW
                     ATGHLGADVISLIGTDPEKNKDSAWKVVQELKIHGAGNLFVKTHPKSKNLWADAPQNP
                     EKDIAESVAVYDLNDLTKKPEIINIAKLAGLPESKAIKRAVHPEYDATGTEVWISLWA
                     GKTEPSAIVVLDDATRKLKKVITDPNMITPTGKFNVTNTQHDIY"
     gene            4185..>4991
                     /locus_tag="PLQ11_10555"
     CDS             4185..>4991
                     /locus_tag="PLQ11_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008548291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HRK25382.1"
                     /translation="MSDQPSASKFPTRAWQTLRKPSLRYSLMALLSGGFIAGILFWGG
                     FNTVMEATNTMTFCTSCHEMRENVYKEYDKTIHHSNRTGVQATCSDCHVPKEWTHKMV
                     RKVQASNEVFHWLLGSVNTPEKFDAKRLTLAKNVWHQMKNTDSRECRNCHTLESMNPE
                     FQRPRARQQHMAAMEQGNTCIDCHKGIAHKNVRPLLTDKEIDELEKPVAAYKREIPAT
                     YKEGLAKAALRETEAEDKRKAEIKTAVDAEVASKLREALAKAAPAAAAAPT"
BASE COUNT          983 a         1654 c         1546 g          808 t
ORIGIN      
        1 accgacctgc gcgccgccca ccagcggcag gcgcggctga ccaacgaggc gctgaacgcc
       61 cgcggcctgc cggtgatgga gaatccatcg catatcgtgc cggttctggt cggcgatgcg
      121 gaacgctgca aggcggcgtc cgaccggctc atggagcggc atggcatcta catccagccg
      181 atcaactatc cgacggtgcc gcgcggaacc gagcggctga ggatcacgcc gacgccgctg
      241 cacaccgatg cccatatcgc ccggcttgcc ggcgcgctcg acgaggtctg gtcggcgctt
      301 ggacttcagc gccgcaggca gggcgccgac acgctcatcg cagcggaata gggaccgatg
      361 ccgaatccgt ccctcacccc catgccggcg acgcaggagg accattggac catccggcat
      421 tcctctctgt tcggtcgcct gccggacacg acgttccgga ccctgttcga gggcaagccg
      481 gtgcatgtgg ccggcaaggc catgccgctg ttcgcctggg ggacgccggc cagaacctgc
      541 ttcatcgtac tgaaggggct cgtcaagctg ttccgcacgt cggaaaccgg ggaagctgcc
      601 gtgctgtcga tctgcggtcc gtgccgaccg ctgatgctgg ccgagggact gaccgccaag
      661 ccctattcgg ccagcgccga gacggtcagc ccggtgcgta tcatgtgtct tgacgtcgcc
      721 ggtctgcggc agagcatggc cgaaaacagc aaactgtcga tggccctgct cgccgccgcc
      781 gcatcggacc tgcgatcgct ggtcgcccat gtcgaggaac tcaaggcgat gaccgggccg
      841 gcccggctcg ccgcgatgat cctcaacctg tccgaggctc gcgccggggc catgcagatc
      901 acgcttccct atgaaaagca gctgatcgcc ggccgcctcg gcatgacgcc ggagagcttc
      961 tcgcgcgcca tgcgccagct caaggaccag ggggtcaagg tcagccgcga ccgtctggtg
     1021 atcaccgata tcgagcgcct gcgcggattt ctcaacacga gggtctgagc aaccgccatg
     1081 tcacccctgc cgcgccgtct ttcccggtcg tgcgtaggcc gccaaaccgg ccacatcagc
     1141 catcagcacc tgatcgcgca tgactgtcac gccgagtttg cgcaggctcg ccagcgcccg
     1201 ggaaaggctc tccggcgtca tgccgaggcg ggcggccagc agcgcctttt cgtagggcag
     1261 gacaacggtt gcggtggcgg cgccgggcac ggtctgctgg accaggaaat cggccagccg
     1321 ccgctgggtg gacatgagct tcaggctggc gatttcggcg aacagccgct cggtatgctg
     1381 caccaccgaa ccgagcaggg ttgccgcaag gcccggctcg gcctcgatcg cctcgttgaa
     1441 ccgcccggcg tcgatgcgca gcagccgcag cgtgctggcc gcctcggcgc tggccggata
     1501 ggtgccgccg agaaagaaca ccgcctcggc aaaactttcg ccgcgcgtga atacgccgac
     1561 gatcgcctcg tcgccggagg ggctgaggcg aaacagcttc acccagccgt caagcaccag
     1621 atagaacccc tccgcctgat cgccctgggc aaatatcaac tggcgcggcg catagctgat
     1681 cggatgctga tggccgcaca ggccttccag cacgcgatgg tcaacctcgg caaacagcgc
     1741 cgaggaccgg acggtctgcc agtcccggct gtcaagtgcg atgccggccg gccggctgtc
     1801 ggtctcaagc ttcaagcaac acccctcatg ccgctggcca acaacaacct cgcccttgcg
     1861 cgcgcgtcaa ggtggcagtg gcggatgcgt ggcccgatcg gcgccatggt cgttgttgca
     1921 gcatcggcgt ggcccctcgc ctgcctggcc cagcgcgacg gcgttgccga tgctctggcc
     1981 ggccatcagg ctgaaatgaa ccagcttgcc ggcaaggacc cgatgcgccg cgcctatttc
     2041 ttcgatccgg ttgctgtcga aggccccgat ccgggcgtgc catgcgatca catcgcccag
     2101 cgccggccgg aggcggtggc ggggccgggc aaggtcgtcc tgctcgaatc ggtggccgag
     2161 gccggccgga tgctgcgatt ctggttgccc ccgggcgccg aaggcagcag cgacccggtg
     2221 gctgcggcgg ccgattatgc cggagagtcc ggcttcgggc cctatcgcct tatcggtgtg
     2281 cggcaattta gctggtgccg ctgaaatcgg cgcaaaactt accgcttctc aattacgtga
     2341 ccgcgcacct caatagtttg cctcgctgta tcattcactt cgaggaggtg tgcgtcatga
     2401 ccctgaagac gcaaggggca aaaatgctgg ggattgcgct tggcgcattc ctgtcaacca
     2461 cggcattcac cccgctttat gcacagcagg cagcaccagc ggccgctacg ccaacattgt
     2521 ccaaggaggc gatggaagcc tccggcaaca tctatttcca gcgctgcgcc ggctgtcacg
     2581 gcgttttgcg caagggggcg acgggcaaga acctcgagcc gcagaacagc gccaagctcg
     2641 gccaggagcg gcttgagaag atcatcgccc tcggcaccga tggcggcatg gtcaactttg
     2701 acgacatcct gaccaaggac gagatcaaga atatcgcgac ctacatccag atgccgccgc
     2761 agtctccgcc cgaatggggc atgaaggaga tgatggcctc ctggaagaac atcgtgccgc
     2821 cggatcagcg tccgaagcag cagatgaaca agatcaacct caagaacgtg ttctcggtca
     2881 ccttgcgtga tgcgggcgag gtcgcgctca tcgacggcga caccaagcag atcatcagcc
     2941 gcgtcaagac cggctatgcg gtccatatct cgcgtctgtc ggcctctggt cgctatgtct
     3001 acgtcatcgg ccgcgatggc ctgctgtcgc tgatcgacct gtggatggag aagcccgctg
     3061 tcgtggccga aatgaagtcc ggtctcgatg cgcgttcggt cgacgtgtcg aagttcaagg
     3121 gcttcgagga caagtatgcg atcgccggct cctactggcc gccgcaatac gtgatcatgg
     3181 aaggcgacac gctgaagccg ctcaaggtcg tctcgacccg tggcgtcacg gtggatggcg
     3241 aataccaccc cgagccgcgc gtcgcctcga tcgtcgcctc gatgatcaag ccggaatggg
     3301 tggtcaacat caaggaatcg ggcctgatcc agctcgtcga ttactccgac atcaagaacc
     3361 tcaagaccac gaccatcgag tcggcgaagt tcctgcatga cggcggctgg gatgcttcca
     3421 agcgctactt cctcgtcgcg gccaacgcct ccaacaaggt ggcggtcgtc gataccaagg
     3481 aaggcaagct ggcggctctg gtcgataccg ccaagatccc gcatccgggt cgcggcgcga
     3541 acttcaccca tccgaagttc ggtccggtct gggcgacggg ccatctgggc gccgacgtga
     3601 tctcgctgat cggcaccgat cccgagaaga acaaggactc ggcctggaag gtggttcagg
     3661 aactgaagat ccacggcgcg ggcaacctgt tcgtcaagac ccatcccaag tccaagaatc
     3721 tctgggccga tgcgccgcag aacccggaaa aggacatcgc cgaatcggtc gctgtctatg
     3781 atctcaatga cctcaccaag aagccggaga tcatcaacat cgccaagctc gccggcctgc
     3841 cggagtccaa ggcgatcaag cgcgccgtgc atcccgaata tgatgccacc ggcacggaag
     3901 tgtggatctc gctgtgggcc ggcaagacag agccctcggc catcgtcgtg ctcgacgatg
     3961 cgacccgcaa gctgaagaag gtcatcaccg acccgaacat gatcaccccg acgggcaaat
     4021 tcaacgtcac caacacccag cacgacatct attgatccgt gccggaaacg catctcgggg
     4081 cggccttcgg gccgcccttt ttttgtcggc gcttatcaac gctcaaggct ccccagggcc
     4141 gccgatggca atggtgcgga caattattcc ggggagccat gcccatgtcc gaccagccct
     4201 cagccagcaa gtttcccacc cgcgcctggc agacgctgcg caagccgtcg ctgcgctatt
     4261 cgctgatggc cctgctgagc ggaggcttca ttgccggcat cctgttctgg ggcggcttca
     4321 acaccgtcat ggaagccacc aacacgatga cattctgcac ctcgtgtcac gagatgcgcg
     4381 aaaacgtcta caaggaatac gacaagacca tccatcactc caaccgcacc ggcgtgcagg
     4441 caacctgttc cgactgtcat gtgccgaagg agtggacgca caagatggtg cgcaaggtcc
     4501 aggcctcgaa cgaggtgttc cactggctgc tcggctcggt caataccccc gagaagttcg
     4561 acgccaagcg gctgacgctg gcaaagaacg tctggcacca gatgaagaac accgacagtc
     4621 gcgaatgccg caactgccac acgctcgaat cgatgaaccc cgaattccag cgcccccgcg
     4681 cccgccagca gcacatggcg gcgatggaac agggcaacac ctgcatcgac tgccacaagg
     4741 gcatcgcgca caagaatgtg cgcccgctgc tgaccgacaa ggaaatcgac gaactcgaaa
     4801 agccggttgc cgcttacaag cgggaaatcc cggccaccta caaggaggga ctggcaaagg
     4861 ccgcccttcg cgagacggaa gccgaggaca agcgcaaggc cgagatcaag accgcggtgg
     4921 atgccgaagt ggccagcaag ctcagggagg ccctggccaa ggcggcgcct gccgctgctg
     4981 cggctccgac c
//
LOCUS       DAOMXG010000174         4967 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_9052_length_4967_cov_9.097720, whole genome shotgun sequence.
ACCESSION   DAOMXG010000174 DAOMXG010000000
VERSION     DAOMXG010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4967
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9052_length_4967_cov_9.097720"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="PLQ11_10560"
     CDS             <1..267
                     /locus_tag="PLQ11_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HRK25383.1"
                     /translation="PGAPGVASEPHPGTVRSPMVGTAYRRPSPDAKPFVEVGSQVKAG
                     DKVLLIEAMKTFNEIVAPRGGTITQLLVDDAQPVEFGEPLFVIE"
     gene            281..1642
                     /gene="accC"
                     /locus_tag="PLQ11_10565"
     CDS             281..1642
                     /gene="accC"
                     /locus_tag="PLQ11_10565"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HRK25384.1"
                     /translation="MFDKVLIANRGEIALRILRACKELGIATVAVHSTADADAMHVKL
                     ADESVCIGPPAARDSYLNIPSLIAACEITGADAVHPGYGFLSENARFAEVLAGHKIGF
                     IGPKAEHIRTMGDKIEAKRTAMRLGIPCVPGSEGGISDDDEALRVAQSIGYPVLIKAA
                     AGGGGRGMKVARTEADLVQALHMAKSEAKAAFGDDAVYIEKYLEKPRHIEVQVLGDGK
                     GHAIHLGERDCSLQRRHQKVWEESPSPALNASQRADIGDVVARAMQELGYLGAGTIEF
                     LYENGQFYFIEMNTRIQVEHPVTEMVTGIDLVNEQIRVAAGGSLSIRQEDVVFDGHAI
                     ECRVNAEHPATFRPSPGRISYFHPPGGLGVRVDSAVYQGYNIPPHYDSLVGKLIVHGR
                     TRTECLMRLRRALDEFVIDGIETTLPLFRTLVRNADIQNGAYDIHWLEKFLRDGGMGK
                     IEG"
     gene            1729..2427
                     /gene="aat"
                     /locus_tag="PLQ11_10570"
     CDS             1729..2427
                     /gene="aat"
                     /locus_tag="PLQ11_10570"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HRK25385.1"
                     /translation="MSTDSILLTPDLVLRAYAAGIFPMAESADDAAVHWVEPRQRGII
                     PLDGFHVPSRLARLVRSDRFEVRVNHDFDAVIEACAAPAPGREVTWINATIRRLYREL
                     FSIGHCHTVEVWRDGRLVGGLYGVHLGAAFFGESMFHRMTDASKVALVHLVGRLRAGG
                     FHLLDAQFMTEHLKQFGTIEVPQQSYLRLLRPAIAAKGDFEIWPRNESVSGARVLDAL
                     PVSSPSLPDPCRPA"
     gene            complement(2387..3013)
                     /locus_tag="PLQ11_10575"
     CDS             complement(2387..3013)
                     /locus_tag="PLQ11_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020091667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2155 domain-containing protein"
                     /protein_id="HRK25386.1"
                     /translation="MPRFGFSFAAAFGLAVLASAPGHADKIKNPVAVFAGLDKITGRI
                     IAFEVAINETVQFGSLQLTPRVCYSRPPYETPRTDVFVEVEEVTFSNEYKRIFTGWMF
                     AASPGLNAVEHAVYDLWLTDCKGGTDIIKVAPEKEEEPQIMPVETKRPARPVQPLERK
                     ADSPLNRPQPSQSFFPTNQPPAIAPPPAAGNRPLVITPAGKDPAGSGN"
     gene            complement(3123..3344)
                     /locus_tag="PLQ11_10580"
     CDS             complement(3123..3344)
                     /locus_tag="PLQ11_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25387.1"
                     /translation="MVKVKHRRNQAYQDVRLVRAASAAGLAFGLMEPEPTNAERIAAL
                     RAMRLMVAVFVPMALVLPLAAVYSFGFVS"
     gene            3512..>4967
                     /locus_tag="PLQ11_10585"
     CDS             3512..>4967
                     /locus_tag="PLQ11_10585"
                     /inference="COORDINATES: protein motif:HMM:NF017591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoX family phosphatase"
                     /protein_id="HRK25388.1"
                     /translation="MDHKRPSSTPLSALIAERLSRRQMLAGTAAGMAALTMPLETLAQ
                     AAPPPFPELTAGLDDKDHVAEGHQIQVVIRWGDPVVPGAPAFDPKAQTGTSQQMLWGH
                     NNDYLCFVPLPGAANPSEHGLLCANHEYASHELMFPGIPAKDIQAFRAQITREMAEVE
                     MAAIGGSIVEIRKDAGRWSVNANSRFNRRLHANTPFTLTGTAAGHPRLRTKADPDGRT
                     ALGMFGCCAGGMTPWGTWLAGEENIQQFFTGKLPDGHPEAENHKRYGGNGRYNWGKFV
                     DRFDVTKEPNEINRAGWVIEIDPLNPDARPRKHTALGRCKHEGATVVINKDGRVVVYS
                     GDDERYEFLYRFVSRGRFDPANRAANLDLLTEGDLSVARYNKDGTVDWLPLRHGAGPL
                     TEAGGFASQADVLIDARFAGRKLGATPLDRPEDIEINPATGKIYVALTNNDRRKPEEV
                     DAVNPRANNRYGHIVEMLPPAGDHAADRFTWEIFL"
BASE COUNT          899 a         1642 c         1579 g          847 t
ORIGIN      
        1 cccggagcgc cgggagtggc gagcgagccg catccgggaa ccgtgcgctc gccgatggtg
       61 ggcaccgcct accgccgccc ctcgcccgat gccaagccgt ttgtcgaggt cggcagccag
      121 gtgaaggcgg gtgacaaggt gctgctgatc gaggcgatga aaaccttcaa cgagatcgtc
      181 gccccgcgcg gcggcaccat cacccagttg ctggtggatg atgcccagcc ggtggaattc
      241 ggcgaaccgc tttttgtgat cgagtgagcg gacgggcgcc atgttcgaca aggtgctgat
      301 cgcaaaccgg ggtgagatcg ccctgcgcat cctgcgcgcc tgcaaggaac tcggcattgc
      361 cacggtggcg gtgcactcga ccgccgatgc cgacgccatg catgtcaagc tcgccgacga
      421 gagcgtctgc atcggcccgc cggccgctcg cgacagctac ctgaacatcc cctcgctgat
      481 tgccgcatgc gagataaccg gtgccgatgc cgtgcatccg ggctatggat tcctgtcgga
      541 aaatgcccgc ttcgccgaag ttctggccgg ccacaaaatt ggcttcatcg gccccaaggc
      601 cgagcatatc cgcacgatgg gcgacaagat cgaggccaag cgcaccgcga tgcggcttgg
      661 tatcccctgc gtgcccggct ccgaaggcgg catttccgat gatgatgaag cgctgcgcgt
      721 tgcccagtcg atcggctatc cggttctgat caaggcggcg gcaggcggcg gcgggcgcgg
      781 catgaaggtc gcgcgcaccg aagccgatct ggtgcaggcg ctgcacatgg ccaagtccga
      841 agccaaggcg gccttcggcg atgacgcggt ctatatcgag aaatacctcg aaaagccgcg
      901 ccatatcgaa gttcaggtgc ttggcgacgg caagggccat gccatccatc tcggcgagcg
      961 cgactgttcc ctgcagcgcc gccatcagaa ggtgtgggag gagagcccct cgccggcgct
     1021 caacgcctct cagcgcgcgg acatcggcga tgtcgtggcc cgcgccatgc aggaactggg
     1081 ctatctcggc gccggcacca tcgagtttct ctacgagaac gggcagttct atttcatcga
     1141 gatgaacacc cgcatccagg tcgagcatcc ggtgaccgag atggtcaccg gcattgatct
     1201 tgtcaacgag cagatccgtg tcgcggccgg tggcagcctg tccatccgcc aggaggatgt
     1261 ggtgttcgac ggccacgcca tcgaatgccg cgtcaatgcc gagcatccgg ccacgttccg
     1321 tccttcgccg gggcggatca gctatttcca cccgccggga ggattgggcg tgcgtgtcga
     1381 ttcggcggtc tatcagggct acaacatccc gccgcactat gactctctgg tcggcaagct
     1441 gatcgtgcac ggccgcaccc gaaccgaatg cctgatgcgt ctgcggcgcg cgctcgacga
     1501 gttcgtcatc gacggtatcg agacaaccct gccgctgttc cgcacgctgg tgcgcaacgc
     1561 cgacatccag aacggcgcct atgacatcca ctggctggag aaattcctgc gcgacggcgg
     1621 catgggcaag atcgagggct gaggccgaag atcagggatg acggtgcggc ctctccgccc
     1681 ctcggccaag gctttccctg gcccggcagc gtccctacat tggtcggcat gagcactgac
     1741 tccatcctgc tgacccccga tcttgtgctg cgcgcctatg cggccggcat tttcccgatg
     1801 gccgaaagcg ctgacgatgc cgccgtgcac tgggtcgagc cgcgccagcg ggggatcatt
     1861 ccgctcgacg gcttccatgt tccgtcacgg ctggcgcggc tggtgcggtc cgatcgtttc
     1921 gaagtgcggg tgaaccacga tttcgacgcg gtgatcgagg cctgcgctgc ccccgctccg
     1981 gggcgcgaag taacctggat caacgccacc atccgccggc tctaccggga gctgttcagc
     2041 atcggccatt gccacacggt cgaggtctgg cgggatggcc ggctggtcgg cgggctctac
     2101 ggcgtgcatc tcggcgcggc cttcttcggc gagagcatgt tccatcgcat gaccgatgca
     2161 tcgaaagtcg cgctggtgca tctggtcgga cggctgaggg cgggcggctt tcacctgctc
     2221 gatgcccagt tcatgaccga acatctcaag cagttcggca cgatcgaggt tccgcagcag
     2281 agctatctca ggctgctgag gccggccatc gccgcaaagg gtgacttcga gatctggccg
     2341 cgcaacgaat ccgtgtccgg cgcgcgcgtg ctcgacgctc ttcccgtcag ttccccgagc
     2401 ctgccggatc cttgccggcc ggcgtgatca ccagggggcg gttcccggcg gcaggcggcg
     2461 gcgcgatggc cggcggctgg ttggtcggga agaacgactg cgacggctgc ggacgattca
     2521 gcggcgaatc tgccttgcgc tccagcggtt gcacggggcg cgccggccgc ttggtttcga
     2581 ccggcatgat ctgcggctct tcttcctttt ccggcgcgac cttgatgatg tcggtgccgc
     2641 ccttgcaatc ggtgagccag agatcataga ccgcgtgctc cacggcattg aggccgggac
     2701 tggcggcaaa catccagccg gtgaagatgc gcttgtattc gttcgagaag gtcacctcct
     2761 cgacttcgac gaacacatcg gtgcgcggcg tctcgtaggg cgggcgcgaa tagcagacgc
     2821 gcggggtcag ctgcagcgag ccgaactgca cggtctcgtt gatcgccacc tcgaaggcga
     2881 tgatgcggcc ggtgatcttg tcgagcccgg caaagacggc gaccgggttc ttgatcttgt
     2941 cggcatggcc gggcgcgctc gccagaacgg caagaccgaa ggcagcggca aaggaaaacc
     3001 cgaaacgggg catgaccgga tccgtcaaag tcaggacgac aggctggggt atttcaccct
     3061 tttcagaacc ggtaaactgg aaattgcggc caaccgatgg cgcagcgcgt caggagcccg
     3121 gttcaggaaa caaatccgaa cgaatagacc gccgccagcg gcagaaccag cgccatcggc
     3181 acgaacactg ccaccatcag ccgcatggcc cgcaaggcag cgattcgctc ggcattggtc
     3241 ggttccggct ccatcagacc gaaggcaagc ccggccgcac tcgctgcccg gaccagccgc
     3301 acatcctgat aggcctgatt ccgcctgtgc ttgaccttga ccatcttccg gcgccccttg
     3361 ctgatgcaca aattgagcat ggcaccggtt aacaggggct tgttccgatg caccaaatcg
     3421 cgctgccgag cgggactttt gcaaatagcc gttgtcatcc cgccgtcatg gtgctccgat
     3481 acccggaaat acaccgggga aggaaatcgt catggaccac aagcgcccat cctccacgcc
     3541 tctgtcggcg ttgattgccg aacgcctgag ccgtcgccag atgctcgccg gcacggctgc
     3601 cggaatggcg gcgctgacca tgccgctgga gacgctggcg caagctgcgc ccccgccctt
     3661 ccccgaactg accgccggcc tcgatgacaa ggatcatgtg gccgagggcc accagatcca
     3721 ggtggtaatc cgctggggcg atccggtcgt gccgggcgcc ccggctttcg atccgaaggc
     3781 gcagaccggc accagccagc agatgctatg gggccacaac aacgactacc tgtgcttcgt
     3841 gcctttgccc ggcgcggcca atcccagcga acacggcctg ctctgcgcca accacgaata
     3901 tgcctcgcat gagctgatgt tccccggcat cccggccaag gacatccagg cgttccgcgc
     3961 ccagatcacc agggaaatgg ccgaagtcga gatggcggca atcggcggct cgatcgtcga
     4021 gatccgcaag gacgccggcc gctggagcgt caacgccaat tcccgcttca atcgccggct
     4081 gcatgccaat actccgttca ccctgaccgg cacagccgct ggccacccgc gactgcgcac
     4141 caaggccgat ccggacgggc gcaccgcgct gggcatgttc ggctgttgcg ccggcggcat
     4201 gaccccctgg ggcacctggc tggcgggcga ggaaaacatc cagcagttct tcaccggcaa
     4261 gctgcccgac ggccatccgg aagcggagaa ccacaagcgc tatggcggca atggccgcta
     4321 caactggggc aagttcgtcg atcgttttga tgtgaccaag gagcccaacg agatcaatcg
     4381 cgccggctgg gtcatcgaga tcgatccgct caatcccgac gcgcgcccgc gcaagcacac
     4441 ggcacttggc cgctgcaagc atgagggcgc caccgttgtc atcaacaagg acggccgggt
     4501 tgtcgtctat tccggcgatg acgagcgtta cgagttcctc taccggtttg tttcgcgcgg
     4561 caggttcgat ccggccaatc gcgccgccaa tctcgacctg ctgaccgaag gcgatctgtc
     4621 ggtcgcccgc tacaacaagg acggcacggt tgactggctg ccgctcaggc atggcgcagg
     4681 cccgctcacc gaggccggtg gctttgccag ccaggccgat gtgctgattg atgcgcgctt
     4741 tgccgggcgc aagctcggcg ccaccccgct cgatcgcccc gaggacatcg agatcaatcc
     4801 ggccaccggc aagatctatg tggcgctgac caacaacgac cgtcgcaagc ccgaagaggt
     4861 cgatgcggtc aatccgcgcg ccaacaaccg ctacggccat atcgtcgaaa tgctgccgcc
     4921 cgccggcgat catgccgccg accgcttcac ctgggaaatc ttcctgc
//
LOCUS       DAOMXG010000175         4949 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_9100_length_4949_cov_10.264201, whole genome shotgun sequence.
ACCESSION   DAOMXG010000175 DAOMXG010000000
VERSION     DAOMXG010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4949
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9100_length_4949_cov_10.264201"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="PLQ11_10590"
     CDS             complement(<1..833)
                     /locus_tag="PLQ11_10590"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HRK25389.1"
                     /translation="MSKAKDTHDVAVVGGGAIGLATALALGQAGIRTILIGAATAIRE
                     DGRTAALMQPSIELLNGLGVMDDLAGVSWPLAAIRLIDITGSLIRSPTVTFRASEIDL
                     PHFALNISNADIVRVMTEAARTCPNLTVETRLVSKVAYCADRVELALTGGDTIVARLV
                     VAADGQKSMLREAAGIEVKRWSYDQVALTFHVTHSRDHEDVSVEFHTRSGPLTFVPYG
                     SFKSSVVWLVSQSEAESLQAAAGGGRTCPAPRVQPARGSDHRRRHRRRADGGAHRPPC
                     SR"
     gene            1055..1831
                     /locus_tag="PLQ11_10595"
     CDS             1055..1831
                     /locus_tag="PLQ11_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HRK25390.1"
                     /translation="MLEAIFSPEALAALVALTAMEIILGIDNVVFISVLVSRLPPEQA
                     ERARKYGLMLALVMRIALLFTLTTLLKLTQPAFTIFGHGVSWRDLILLFGGLFLVVKA
                     THEMHLEMEGGEHLVDGNVKVRTFGAAMVQIAIIDLVFSVDSIITAIGMARDLWVMVV
                     AVILSMIVMYLAAGPVSAFVHRHPTAKMLALAFLVLIGVALIAESVHSPIDRKIIYGA
                     MAFSVLVEMVNLRAARNRGRRNPVPHQDSGGGASPNKARE"
     gene            complement(1786..3474)
                     /locus_tag="PLQ11_10600"
     CDS             complement(1786..3474)
                     /locus_tag="PLQ11_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HRK25391.1"
                     /translation="MGKVSAAVSEVFQPKIVTCLKEGYDVTRLRADALAGLTVAIVAL
                     PLSMAIAIASGASPERGLYAAIVGGFLISLLGGSRHQIGGPAGAFIVLVAAVIERHGL
                     DGMFLATIMAGAIMIGLGTFRLGTLIRYIPEPVIIGFTAGIAVIIFASQLREILGLAL
                     TVKEPAALVPKLGVLWQALPSVNPYAIGVATFAFAIIKLGPKVGPSFPGFLVAVIAAS
                     ALVWGFNLPVETVASRFGVVPSGLPAPAMPPFGFEKIHAVAIDALAIALLGSIESLLS
                     AVVADSMTGRRHRSNTELIAQGFANIITALFGGLCATGTIARTATNVRSGGTSPIAGI
                     LHALFLLGAMMIAAPLMGRIPLAALGALLAVVAWNMAEKHAFAALLRGSRAEAAVLLA
                     TFLLTVFRDLTEGIIFGVLLGAVLFAVRMARLAEVSMQMSLSGSDGAAEDALPEADPD
                     SPVMIFRLEGPLFFGTAPNLVDVLGRAGAEPKAVVFDFGSVPFIDATGVAALERHVGK
                     AERGGTRVYFAGVSPQVELGLRQFGLPKARVPRFRTVAEAYSRALLGEAPPPES"
     gene            3647..4621
                     /locus_tag="PLQ11_10605"
     CDS             3647..4621
                     /locus_tag="PLQ11_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HRK25392.1"
                     /translation="MVKAIRVHVCGGPEQMVLEEVALPPPGKGEVRVRHAAMGLNFID
                     VYFRTGLYPAPAMPFTPGNEGAGTVVSVGEGVVGFKPGDRVAYAAQIGSYAEERNVSA
                     HFVVKLPKDIGFESAAGMMLKGMTAEYLLFRTHRVKKGDVVLMHAAAGGVGLILCQWA
                     RSLGATVIGTVGSADKAKLARKHGCHHTILYREENFVERVREITGGKLCDVVYDGVGK
                     DTFLGSLDCLKPLGMFVSFGNASGPIAAFDIGLLAKKGSLVATRPTLFTFLADRKRLV
                     SMARRLFTAVRKGDVTIPVTGRFPLAKAAEAHRALEGRGTTGSIIFTP"
     gene            4635..>4949
                     /locus_tag="PLQ11_10610"
     CDS             4635..>4949
                     /locus_tag="PLQ11_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006934419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRK25393.1"
                     /translation="MSSRDPAAGQDVPLLKCVDLTKRFGALTANDSVSLTVNAGEIHA
                     LLGENGAGKSTLVKQIYGLLKPDSGHIEWQGRRVDLTSPEAARALGIGMVFQHFSLFE
                     DLS"
BASE COUNT          921 a         1561 c         1604 g          863 t
ORIGIN      
        1 cgactgcatg gcgggcgatg agccccccca tcggcacggc gccgatgccg ccgacgatgg
       61 tcagatcccc gagcaggctg gacgcgcggc gctgggcaag ttcggccacc tcctgcggcg
      121 gcctggagcg attcggcctc ggactgactg accagccaga cgaccgagga tttgaaactg
      181 ccgtagggca cgaaggtcag cgggccggag cgggtatgga attcgaccga aacgtcctca
      241 tgatcacgcg aatgcgtgac atggaaggtc aacgccacct gatcatagct ccagcgcttg
      301 acctcgatgc cggctgcctc tcgcaacatc gatttctgcc cgtcggcggc aacaaccagc
      361 cgcgcgacga tcgtgtcacc cccggtcagc gcaagctcca cccggtcggc acaataggcc
      421 accttgctga cgagacgggt ttccactgtg aggttggggc aggtgcgggc cgcctcggtc
      481 atcaccctga cgatatcggc attggagatg ttgagcgcaa aatgcggcag gtcgatctcg
      541 ctggcccgga acgtgactgt cggcgaacgg atgaggctgc cggtgatgtc gatcagccgg
      601 atggcggcga gcggccagga gacgcctgcc agatcgtcca tcacaccaag cccgttgagc
      661 agttcgatgc tcggctgcat cagcgcggca gtgcggccat cttcccggat cgcggtcgcg
      721 gccccgatga gaatcgtgcg gatgccggcc tgacccagcg caagcgccgt ggccagcccg
      781 atggcaccgc cgccaacgac cgctacatca tgggtgtctt ttgccttgct catcgcggag
      841 attctccata gatcatgctg tcgttttgat tgaaacatca aaacgtgaga taacatgatc
      901 gttatcaata gcttagagca tgcttgttgc gattagccgg attccaccta atcgcagcat
      961 gctctaggcg gccttgtcat tcgctacaac acgaaccaac ttcgcatcac tggcaagcgc
     1021 gccgtttgcc gctacgacca attggggagt ttcaatgctc gaagcgattt tttcacccga
     1081 agcactggct gcgctggtcg cgctgactgc catggagatc attctcggga tcgacaacgt
     1141 cgtgttcatc tcggttctgg tctcccgcct gccgcctgag caggcagaaa gagcgcgcaa
     1201 atacggcctg atgttggcgc tggtgatgcg catagcgctt ttgttcacgc tgacgaccct
     1261 gctcaagctg acccagccgg ccttcaccat tttcggccac ggtgtctcct ggcgggatct
     1321 gatccttctc ttcggcggtc tgttcctggt ggtcaaggcg acgcacgaga tgcacctgga
     1381 gatggagggc ggcgagcacc tggtcgacgg caatgtcaag gtgcgcacat tcggtgcggc
     1441 gatggtgcag atcgccatca tcgatctggt cttttcggtc gactcgatca tcaccgcgat
     1501 cggcatggcg cgcgatctgt gggtgatggt tgtggcggtc atcctgtcga tgatcgtgat
     1561 gtatctggcg gcggggccgg tttcggcctt cgtgcatcgc cacccgactg ccaagatgct
     1621 ggcgctggcc ttcctggtgc tgatcggcgt ggccttgatc gccgaaagcg tccacagccc
     1681 gatcgaccgc aagatcatct atggcgccat ggctttttcg gttctggtcg aaatggtcaa
     1741 cctcagggct gccaggaacc gcggacgccg caaccctgtc ccgcatcagg actcgggcgg
     1801 cggagcctcg cccaacaagg cgcgcgaata ggcctcggcc acggtccgaa agcgcggcac
     1861 ccgcgctttc ggcagtccga actggcgaag ccccagttcg acctggggcg acacgccggc
     1921 gaaatagacc cgggtgccgc cgcgctcggc cttgccgaca tggcgctcga gcgcggcaac
     1981 gccggtggcg tcgatgaacg gcaccgagcc gaaatcgaac accaccgcct tgggttcggc
     2041 gccggcacgg ccgagaacat cgacaaggtt gggggccgtc ccgaagaaca gcggcccttc
     2101 cagccggaaa atcatcaccg gactgtcggg atcggcttcg ggcagcgcat cctccgccgc
     2161 cccgtcgctg ccgctcagcg acatctgcat cgacacctcc gccagccgcg ccatgcgcac
     2221 cgcaaacagc accgcgccaa gcagaacgcc gaagatgatg ccctcggtca gatcgcggaa
     2281 aaccgtcagc aggaaggttg ccagcagcac cgccgcctcg gcgcggctgc cgcgcagcaa
     2341 cgccgcgaaa gcgtgctttt ccgccatgtt ccaggcaacg accgcaagca gtgcgccaag
     2401 cgcggccagc ggaatgcggc ccatcagcgg ggcggcgatc atcatcgcgc cgagcaggaa
     2461 caatgcatgc aggatcccgg cgatggggct ggtgccgcct gaacggacat tggtggccgt
     2521 gcgtgcaatg gttccggtgg cgcagagacc gccgaacagc gccgtgatga tattggcaaa
     2581 gccctgcgcg atcagttcgg tattggagcg atggcgacgc ccggtcatgc tgtcggccac
     2641 cacggcggac agcagcgact cgatcgagcc gagcagggcg atggccagcg cgtcgatcgc
     2701 caccgcatgg attttctcaa acccgaatgg cggcatggca ggagccggca gcccggacgg
     2761 cacgacgccg aagcggctcg ccaccgtctc gaccggcagg ttgaaccccc ataccagagc
     2821 ggaggcggca atcaccgcca ccagaaaacc gggaaaactc ggccccacct tcggccccag
     2881 cttgatgatc gcaaaggcga aggtcgccac gccgatcgcg taggggttga cgctgggcag
     2941 cgcctgccac agcacgccga gctttgggac cagcgccgcc ggctccttga ccgtcagcgc
     3001 cagcccgagg atctcgcgca gctggctggc gaagatgatc actgcgatgc cggcggtaaa
     3061 gccgatgatc accggttcgg gaatgtagcg gatcagcgtg ccgagccgga aggtgcccag
     3121 gccgatcatg atcgccccgg ccatgatggt ggcgagaaac attccgtcga ggccatggcg
     3181 ctcgatgacc gcagcaacca gcacgatgaa tgcgcctgcc ggcccgccga tctggtggcg
     3241 cgaaccgccg agcagtgaaa tcaggaaccc gccgacgatc gcggcataaa gcccccgttc
     3301 gggcgatgcg cctgaggcga tcgcaatcgc catcgacagg ggcagggcga cgatggcaac
     3361 tgtcagcccg gccagcgcat cggcacgcaa gcgcgtgaca tcgtagcctt ccttgagaca
     3421 ggtgacgatt ttcggctgga acacttcgct gaccgccgcg ctgaccttgc ccatcgttgc
     3481 cctgtccaag aaattcttgt tttcgtcagc gttgttgcgg gccgactcgg cagcatttga
     3541 cgccgacttg ccggcaaatg caaaatcctt ggaaggcgcg acaacagcac tggaaactcg
     3601 ggcgccatgt cgtaaggtga cggtgctgat catgaggggt caaaaaatgg tcaaagccat
     3661 tcgtgtgcat gtgtgcggcg gtccggagca gatggtgctg gaggaggtcg cgctgccgcc
     3721 gcccggcaag ggcgaggtgc gtgtccggca cgcggcaatg gggctgaatt tcatcgatgt
     3781 ctatttccgc accgggcttt acccggcccc cgcgatgccg ttcacccctg gcaacgaggg
     3841 cgccggaacg gtcgtttcgg ttggggaagg ggttgtcggg ttcaagccgg gcgaccgcgt
     3901 cgcctatgcg gcgcagatcg gctcctatgc cgaggagcgt aacgtctcgg cgcattttgt
     3961 cgtcaagctg ccgaaggaca tcggcttcga atcggcggcc ggcatgatgc tcaagggcat
     4021 gacggcggaa tacctgctgt tccgcaccca tcgggtgaag aagggcgatg tcgtgctgat
     4081 gcatgcggcg gccggcggcg tcgggttgat cctgtgccag tgggcgcgct cgctcggcgc
     4141 gacggtgatc ggcacggtcg gctcggctga caaggccaag cttgccagga agcatggctg
     4201 tcaccacacg atcctttacc gcgaggagaa tttcgtcgaa cgggtcaggg agatcaccgg
     4261 cggcaagctc tgtgatgtgg tgtatgacgg ggtcggcaag gacacgttcc tgggatcgct
     4321 cgattgcctc aagccgctcg gcatgttcgt cagctttggc aacgcctcgg gacctattgc
     4381 cgccttcgac atcggcctct tggccaagaa gggctcgctg gtggcgacgc ggccgacgct
     4441 gttcaccttc ctggccgacc gcaagcgtct ggtcagcatg gcccggagac tgttcacagc
     4501 cgtgcgcaag ggggatgtga ccatcccggt caccgggcgg ttcccgctcg caaaggcggc
     4561 ggaggcgcat cgcgctctgg aagggcgcgg aaccaccggc tcgatcatct tcaccccatg
     4621 atcagcgtca taccatgagc agccgcgatc ccgctgccgg ccaggacgtg ccgctgctga
     4681 aatgcgtcga tctgaccaag cggttcggtg cgctcacggc caatgattcg gtttcgctga
     4741 ccgtcaacgc cggcgaaatc cacgccctgc tgggtgagaa cggggcgggc aaatcgaccc
     4801 tcgtcaagca gatctacggg ttgctaaagc cggattccgg ccacatcgaa tggcagggcc
     4861 ggcgggtgga cctgaccagc ccggaagcgg cgcgggcgct cggcatcggc atggtgttcc
     4921 agcatttctc gctgttcgag gatctcagc
//
LOCUS       DAOMXG010000176         4917 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_9188_length_4917_cov_9.557384, whole genome shotgun sequence.
ACCESSION   DAOMXG010000176 DAOMXG010000000
VERSION     DAOMXG010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4917
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9188_length_4917_cov_9.557384"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="PLQ11_10615"
     CDS             <1..364
                     /locus_tag="PLQ11_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012319872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HRK25394.1"
                     /translation="GDIVDLARGAAVATFGRPAGALEKGARAPMREHEGGYYIRLQVK
                     DRPGAAATIATRMAERGISLESILQKGARDRVQNGASVPVVLITYATHESAIREALAA
                     IAADGVLDGEPQAIRIER"
     gene            complement(377..1525)
                     /locus_tag="PLQ11_10620"
     CDS             complement(377..1525)
                     /locus_tag="PLQ11_10620"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRK25395.1"
                     /translation="MNDRTSAKIPGYRPLGTFRFFLALLVLAQHFTNIAPHTFAALVV
                     PLRLGSLAVLVFFAVSGFVIAEAVETHYRGRPGAFLANRAIRIYPPLLAAFALMLLVL
                     AGVNSVGPMPHPEPWQPNLEPGAVFSGSNLIANLLSFLPAPKVLGEGITYPLIIVAWS
                     IRVEFLFYLVMAAALILPAQWYRPSLVAAGLLALMAFVVHTTGRGPEMLQFMPYFVYG
                     VALYFVVAGARFARPVVLIALTGCAVQALLDTGLNGAAAGIGNAGVDIARVLILAACL
                     LLLPILAREQAGRFRATDQALGDLSYPLYLNHCVLLPVVRSLAPDASVAGLLGATVAA
                     VALSYLMLLAVEPVLTGLRDRIRGSAPALLPMVTGRQRPDQLAKAPLR"
     gene            complement(1710..2432)
                     /locus_tag="PLQ11_10625"
     CDS             complement(1710..2432)
                     /locus_tag="PLQ11_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25396.1"
                     /translation="MLRVITCGLLACLGGLTVTAASARSECPPRAPASTSVICSDTGS
                     AQLDRQIAAAFEAARKRLGEAARGHLLKDQDAFRQVRAHGSNSRDFDLEVNMAMRRDF
                     LNAVQPASGAAWRPHWGNHHGTITVNQQMPGRIVVRVSAMEPTVGAWTCEFDEQAEAV
                     NGQLIVGQASARLELGGPNEGWTLALKRTGDVLIVEEIAPAGASRRPFCTGPGSLAGV
                     YFALQEPEVAKKEAMATGGKAQ"
     gene            2816..3811
                     /gene="glpX"
                     /locus_tag="PLQ11_10630"
     CDS             2816..3811
                     /gene="glpX"
                     /locus_tag="PLQ11_10630"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HRK25397.1"
                     /translation="MTELIVDESKIIERVLAMELVRVTERAAVASAKLRGRGDEKAAD
                     QAAVDAMRRELNRLPIDGEVVIGEGERDEAPMLFIGEKVGTKKGPKVDIAVDPLEGTT
                     LCAKDMPGSIAVMAMAQAGTLLYAPDVYMDKIAIGPGYAKGIVDLDASPEDNIMAMAK
                     AKMVKPGEITALVMDRPRHAELIAKLRKIGCGIRLITDGDVVGVIQCAEQHKTGIDIY
                     LGIGGAPEGVLAAGALRCVGGQMQGRLILDTPEKVARAAKMGVKDPNKKYDLSELASG
                     DTVVAATGVTDGALLRGVKFKGPIIETETIIYRSLTGTVRRIFGEHRELEKFHLG"
     gene            complement(3852..4559)
                     /locus_tag="PLQ11_10635"
     CDS             complement(3852..4559)
                     /locus_tag="PLQ11_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019997646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRK25398.1"
                     /translation="MTNALTMGDRKATPDRLPRSTAATQILIQLRQAILAMDLPPGTP
                     LTEKQLTERFGVSRTPVREALIRLAEERLVDIFPQSGTFVGRIPVSTLPEAVVIRKAL
                     EQAALELAIARADTAALDELERVIARQQAMASIDDRDGFHAADEAFHETIATIAGHPG
                     IWRVAQQAKVQIDRCRRLTLPTPGRMGQVIAEHRAILAAMRKRDGAQARQALDRHLSM
                     VLPDIDTFRQSHPDYFV"
     gene            4656..>4917
                     /locus_tag="PLQ11_10640"
     CDS             4656..>4917
                     /locus_tag="PLQ11_10640"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008127463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HRK25399.1"
                     /translation="MALHIDRLFPAEDRTRAIARELYRGVRAAPIVSPHGHTEPAWYA
                     LNQPFADPATLFVKPDHYVFRMLHSQGVPLEALGVPRADGGAS"
BASE COUNT          962 a         1524 c         1613 g          818 t
ORIGIN      
        1 gggcgacatc gtcgatctgg cgcgcggcgc tgcggtcgcc accttcggcc gcccggccgg
       61 agcgctggaa aagggcgccc gcgcgccgat gcgcgagcac gagggcggct attacatccg
      121 cctgcaggtc aaggatcgtc cgggcgccgc cgcgaccatc gccacccgca tggccgaacg
      181 cggcatcagc ctcgaaagca tcctgcagaa gggcgcgcgc gaccgggtgc agaacggcgc
      241 ctcggttccg gtggtgctga tcacctatgc cacgcacgaa tccgccatcc gcgaggcgct
      301 cgccgccatc gccgccgatg gcgtgctgga cggcgagcct caggcgatcc ggatcgagcg
      361 gtaggcggcg caggtttcag cgaagcggag ccttcgccag ctggtcgggt ctctgccgcc
      421 cggtcaccat gggcagtagg gcaggcgcgc tgccgcggat gcgatcgcgc aggccggtca
      481 gcacgggttc gactgccagc agcatcagat aggacagggc caccgcggcg acggttgcgc
      541 cgagcagtcc ggccacggag gcgtccggcg caagactgcg caccaccggc agcagaacgc
      601 agtggttgag gtagagcgga taggacaggt cgccgagggc ctggtcggtg gcccggaacc
      661 gccccgcctg ttccctggcg aggatcggca gcaacaagag gcaggcggcg aggatcagaa
      721 cgcgggcaat gtcgacgcca gcgttgccga taccggccgc cgccccgttc aggccagtgt
      781 caagcagggc ctgaacggcg cagccggtca gagcaatcaa cacgacgggg cgggcgaagc
      841 gtgcgccggc gaccacgaag tagagtgcca cgccatagac gaaatagggc atgaactgca
      901 gcatttccgg cccgcgcccg gtggtgtgca cgacaaaggc catcaacgcc agcaggccgg
      961 cggccaccag tgagggacga taccactgtg ccggcagtat caacgcggca gccataacca
     1021 gatagaacag gaattcgacg cggatcgacc aggcaacaat gatcagcgga taggtgatgc
     1081 cctcgcccag caccttgggg gccggcagga aactcagcag attggcgatc aggttggacc
     1141 cgctgaacac cgccccgggc tccagattgg gctgccaggg ctctggatgc ggcattggcc
     1201 cgacactgtt gacccccgcc aggaccagca gcatcagcgc gaacgccgcc agcaagggcg
     1261 gatagatgcg gatcgcgcgg ttggccagaa aggcgccagg ccgcccccgg tagtgggttt
     1321 ccaccgcctc cgcgatgaca aagccagaaa cggcaaagaa caccagtacc gccagcgatc
     1381 caagccgtaa tggcaccacc agcgcggcga acgtgtgagg cgcgatgttg gtgaagtgct
     1441 gcgccagaac aagcaacgcg agaaaaaagc ggaacgtgcc caacggacgg tagccaggta
     1501 ttttggcaga ggttctgtcg ttcacggtcg gccccagctt gatgcgtgcc gaaaaatacg
     1561 ttcaaccaga agagagagaa agcagaggct gtctgtttgg taagtgttaa tccttaagaa
     1621 cgttgtttac tatgatcggg ccgtgccccg attgaacacc cgttccatta tcctcggatc
     1681 catgacaggt cagggcagcg gcgttgaggt tactgagctt tgccgccggt agccattgct
     1741 tcctttttgg caacctccgg ctcctgaagc gcgaaataga ccccggccag cgagccgggc
     1801 ccggtgcaga acgggcgtcg gctggcgccg gcgggcgcga tttcctcgac gatcaggacg
     1861 tcgccggttc gcttcagcgc tagggtccag ccttcgtttg ggccgccgag ctccagccgg
     1921 gcgctcgcct gtccgacgat cagctggccg ttgacggcct ctgcctgctc gtcaaattcg
     1981 caggtccagg ccccgaccgt cggctccatc gcgctgacac ggaccacgat ccggcctggc
     2041 atttgctgat tgactgtgat cgtcccgtga tggttgcccc agtgcggacg ccatgcagcg
     2101 ccgctggcgg gctgcaccgc attgagaaag tcgcggcgca tggccatgtt gacttcaaga
     2161 tcgaaatccc tggaattgga accgtgcgcc cgcacctggc ggaaggcatc ctgatctttg
     2221 agcaaatgac cgcgtgcagc ttcgccgagg cgtttgcgtg ccgcttcgaa ggccgcagcg
     2281 atctgccggt caagctgggc actgccggta tcggaacaga tgacggatgt tgatgccggt
     2341 gcgcgcggcg ggcattcgct tctggcagag gcggcggtga cagtcaggcc gccaaggcag
     2401 gccagcaagc cgcaagtgat gacacgcagc atagggtgat ccggtgtgac agcgtcattg
     2461 ttgccgccag tcaagaaaaa gtggttaatg tttcaacccg cattgctggc actgcgtcag
     2521 aaccggcaag acgttgcgtc agccttgctg aaagcactcg cggaatgctg cgaaggcacg
     2581 caatttattg acgaattttc aaccgaaaag cacctttgat cgccgcatcc atatggcaaa
     2641 atggcaagaa tctctatagg agccggtgaa cgcccgcgcc tcccagagca tgcagcgaaa
     2701 aagtggcaac ggtttttcgc ggaatgcatg ctctgaacta ttgtaatcga tcacggcatc
     2761 tcttgttttg atgagtccat caaaacgaga gcgtgatcta accaggaaag ctgacatgac
     2821 cgaactcatc gtcgacgaaa gcaagatcat cgaacgcgtt ctggcgatgg aactggtgcg
     2881 tgtcaccgag cgcgccgctg tggcttcggc caagctgcgc ggccgtggcg atgaaaaggc
     2941 ggccgatcag gcagcggtgg atgcgatgcg ccgcgaactc aaccgcctgc ccatcgacgg
     3001 cgaggtggtg atcggcgaag gcgagcgcga cgaggcgccg atgctgttca tcggcgagaa
     3061 agtcggcacc aagaaggggc cgaaggttga tatcgcggtg gacccgctgg aaggcaccac
     3121 gctctgcgcc aaggacatgc cgggttcgat cgcggtcatg gcgatggccc aggccggcac
     3181 cttgctctac gcgccggacg tctacatgga caagatcgcc atcggccctg gctacgccaa
     3241 gggtatcgtc gatctcgatg cctcgcccga ggacaacatc atggccatgg ccaaagccaa
     3301 gatggtcaag cccggcgaga tcaccgcgct ggtcatggac cggccgcgcc atgccgagct
     3361 gatcgccaag ttgcggaaga tcggctgcgg catccgtctg attaccgatg gcgatgttgt
     3421 cggcgtcatc caatgcgccg agcagcacaa gaccgggatc gacatctatc tggggatcgg
     3481 cggagcgccg gaaggcgttc tggcggcagg cgcattgcgc tgcgtcggcg gccagatgca
     3541 gggccgcctg atcctcgaca cgccggaaaa ggtggcccgc gccgccaaga tgggcgtcaa
     3601 ggatccgaac aagaaatacg acctgtccga actggcctcg ggcgacaccg tcgtcgccgc
     3661 caccggcgtc accgacgggg cgctgctcag gggcgtcaag ttcaagggcc cgatcatcga
     3721 gaccgagacc atcatctatc gctcgctgac cggcacggtc cggcgcattt tcggtgaaca
     3781 tcgcgagttg gagaagttcc acctcggctg aggcgaccgg tgtcatcggt cacttgttgt
     3841 gaccgatggg atcagacgaa ataatccggg tgcgactgcc ggaatgtgtc gatgtccggc
     3901 agaaccatcg acaggtgacg gtcaagcgcc tgccgcgcct gcgctccgtc gcgcttgcgc
     3961 atcgctgcaa gaatggcgcg atgctcggca atgacctgac ccatgcggcc gggggtcggt
     4021 agtgtaaggc gccggcagcg gtcgatctgc accttcgcct gctgggcaac acgccagatg
     4081 ccgggatggc cggcgatggt ggcgatcgtc tcgtgaaagg cttcatcggc cgcgtgaaag
     4141 ccatcgcggt catcaatgct cgccatcgcc tgctggcggg caatcacccg ctccagttca
     4201 tccagcgccg ccgtatcagc ccgcgcgatc gccagttcga gggccgcctg ttccagcgcc
     4261 ttgcggatga ccaccgcctc gggcagcgtg gaaaccggaa tgcggccgac gaaggttccc
     4321 gattgcggga aaatatccac cagccgctct tcagccagcc ggatcagcgc ctcgcgcacc
     4381 ggagtccggc tgacgccgaa acgctccgtc agctgctttt ccgtcagcgg cgttccgggc
     4441 ggcaggtcca tcgccaggat cgcctgccgc aactggatga ggatctgtgt cgccgctgtc
     4501 gatctgggca accggtccgg cgtggctttc ctgtcgccca tcgtaagcgc gtttgtcatg
     4561 cagcggttcc acaatctggt gcccagtcag caatgcaatt gacatactag tatattagtt
     4621 tattgacaag gcgtgattga aggttggcgg ctgccatggc gcttcatatt gacaggctgt
     4681 tccccgcgga ggaccggacg agagccatcg cccgggagct ttatcgcggc gtccgggcgg
     4741 cgccgatcgt cagtccccat ggccataccg aaccggcctg gtatgcgctg aaccagccgt
     4801 ttgccgaccc tgcgacgctg ttcgtcaagc cggaccatta cgtgttccgc atgctccatt
     4861 cgcagggcgt gccgctggag gcgctggggg tgccgcgcgc cgatggcggc gcctcgg
//
LOCUS       DAOMXG010000177         4843 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_9376_length_4843_cov_8.624269, whole genome shotgun sequence.
ACCESSION   DAOMXG010000177 DAOMXG010000000
VERSION     DAOMXG010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4843
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9376_length_4843_cov_8.624269"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="PLQ11_10645"
     CDS             complement(<1..491)
                     /locus_tag="PLQ11_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRK25400.1"
                     /translation="MAAILKDVGFVHLHVHSSYSLLEGALPIATLAKLAAADGMPALA
                     LTDTNNLFGALEFSEKLAGAGIQPIIGCQLIVDFGDREGARSRPGMTSDARGPLVLLA
                     ASEAGYRNLMHLSSVAFLETPSQDTPHLPLTQLAGHCDGLIALTGGFDGPIDRLLRDG
                     QAEA"
     gene            801..1787
                     /locus_tag="PLQ11_10650"
     CDS             801..1787
                     /locus_tag="PLQ11_10650"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HRK25401.1"
                     /translation="MRLPGYAVRGALAVGLLAFAGWAELAILFHAPLSNESRILVATL
                     VAAVLLIGSLSVLARVWRRLILPVVLVVAIGMLAWWSSIKPSDTRDWMPDVAKKAVVT
                     IRGDDVEIRNVRNFTWTGPDTAQEQWQDRIYSLSDVTGLDLFLSTWGNPRIAHAITSF
                     SFSNGPPVAFSFEIRKRKGQDYSAIGGFFKQYELVLIAADEADVVKVRTNRRNETVHR
                     YRIDVKPENATKLLREYARISQELSAEPRWYHTIWTSCSTTIYWMLRRISPDDFPFDL
                     RVLLSGYLPGYLYDIGFLDRKQPLDEIMRRADITALAKQAEGQADFSAAIRK"
     gene            1924..2793
                     /locus_tag="PLQ11_10655"
     CDS             1924..2793
                     /locus_tag="PLQ11_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRK25402.1"
                     /translation="MSDPHSLAPLLTRIAEALERIAPPAPARPDYEAADAFVWSASRQ
                     ALTPVVRVNRIPLELLVGIDRIRDLLVDNTRRFARGLPANNVLLWGARGMGKSSLVKA
                     AHASVNAEVGATMRLKLVEIHREDIETLPALLTTMRTSSHRFLVFCDDLSFDAGETSY
                     KSLKAVLEGGLEGRPANVLFYATSNRRHLMPRDMIDNERATAINPGEAVEEKVSLSDR
                     FGLWLGIHKCSQEEYLAMVHAYARHFGLKLRRTRLDADALEWATTRGSRSGRTAWQFI
                     QDLAGRQGKLLDL"
     gene            3025..3504
                     /gene="ssb"
                     /locus_tag="PLQ11_10660"
     CDS             3025..3504
                     /gene="ssb"
                     /locus_tag="PLQ11_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017960442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRK25403.1"
                     /translation="MAGSLNKVMLIGNLGADPEIRRLPSGGSVANLRIATSESWRDKA
                     TGERREKTEWHNVVVFNDNLVRVIEQYVKKGSKVFVEGQLQTRKYQDKSGQDRYSTEV
                     VLQGFSGNLTLLDGRGDGARDGGDGRGSGDFGRSSPMDRKPSGGGRSTALDDDDIPF"
     gene            3582..4769
                     /locus_tag="PLQ11_10665"
     CDS             3582..4769
                     /locus_tag="PLQ11_10665"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF005262.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HRK25404.1"
                     /translation="MLFSIGKRLAEMAMIALMATGAMAQDMLVEKKTFEMASLTTVSG
                     ATVRNVKVGWEAYGTLNADKSNVILVPHFFSGTSHAAGKYKPDDKLPGYWDYLIGPGK
                     AVDTNRFYVISVDSLVNLNAKDPNVVTTGPASINPDTGKPYGMTFPLVTIRDFVNVQK
                     ALLDSLGIRKLHAVMGASMGGLQSYEWAASYPDMVERLLPVIAAGDPGPWLNAWLNVW
                     ASPILIDANWNKGDYYGKAEPVEGLKTALKIVSLHANHWLWAEKTIGAGWATEGKDPL
                     AAFDNRYKIEATLDAAGAARAAVSDANHFLYLVKANQTFVPGGAKTAAEGLKKIKART
                     LMLYAPTDQVFAADWVRATAEAIRANGTPVETAEIAGNFGHLNGVLALKPLEARIVDF
                     LTK"
BASE COUNT          897 a         1525 c         1521 g          900 t
ORIGIN      
        1 gcctccgcct gcccgtcgcg cagcagccgg tcgatcggcc cgtcaaaccc gccggtcagc
       61 gcgatcagac cgtcacaatg ccccgccagc tgggtaagcg gcagatgcgg cgtgtcctgg
      121 gacggcgttt cgagaaaggc cacgctcgac agatgcatga ggttgcgata gccggcctcg
      181 ctggctgcca gcagcaccag cggaccgcgc gcatcgctgg tcattcccgg acgcgaccgt
      241 gccccctccc gatcgccgaa atcgacgatg agctggcagc cgatgatcgg ctggatgccc
      301 gcccccgcca gcttttcgga aaactccagc gcgccgaaca ggttgttggt atcggtcagc
      361 gccagcgccg gcatgccgtc ggccgccgcc agcttggcga gcgtggcaat cggcagcgcc
      421 ccctccagca acgaatagga ggaatgaacg tgcagatgca cgaaccccac atccttcagg
      481 atggctgcca tacccctcac cttcacgcat gcccggtcag gcgccgagcg ccccggccca
      541 taccagaatc atagccagaa acgcacccag cgccgcaatt tcgatagctt cttccacaag
      601 cattcgcagg aacatgagcc cctcctttgt tctttttatg ttcgtattcc acttttgttc
      661 tcatgtcaaa ttctccgcca aggccggccc tgccgtggtg aatcggccgt aaaccctgcc
      721 tcacccattg cagcgcgggc ctacgccact acaatcggtg catgaactgg ctttcggatt
      781 cgcttgggcc tggactgctg atgcggctgc ctggctatgc cgtgcgcggc gcgctggcgg
      841 tcggcctgct ggcctttgcc ggctgggccg aactggcgat tctgtttcac gcgccgctgt
      901 ctaacgagag ccgtatcctg gtggcgaccc tggtcgccgc ggtgctgctg attggttcgc
      961 tgtcggttct ggccagggtc tggcgccggc tgatcctgcc cgtggtgctg gtggtggcca
     1021 tcggaatgct ggcctggtgg agttcgatca agccgtcgga cacgcgcgac tggatgccgg
     1081 atgtagccaa gaaggccgtc gtcaccatcc gcggcgatga tgtggaaatc cgcaatgtcc
     1141 gcaattttac ctggaccggc ccggacaccg cgcaggagca atggcaggat cgcatctaca
     1201 gcctgtctga tgtcacaggg ctcgacctgt tcctgtcgac ctggggcaat ccgcgcattg
     1261 cccatgcaat caccagcttt tccttttcca acgggccgcc ggtggccttc tccttcgaga
     1321 tccgcaagcg caaggggcag gattattcgg cgatcggcgg tttcttcaag caatacgaac
     1381 tcgtgctgat cgcggctgac gaggcggatg tggtgaaggt ccgcaccaac cgccgcaacg
     1441 aaacggtgca ccgctaccgg atcgacgtga agcccgagaa tgccaccaag ctgctgcgcg
     1501 aatatgcgcg catctcgcag gagctcagcg ccgagccgcg ctggtatcac accatctgga
     1561 ccagttgctc gacgaccatt tactggatgc tgcgccgcat cagcccggat gattttccgt
     1621 ttgacctgcg ggttctgctc tcgggttacc tgccgggcta tctctacgat atcggctttc
     1681 tcgatcgcaa gcagccgctc gacgagatca tgcggcgggc cgatattacg gcgctggcca
     1741 aacaggccga ggggcaggcc gatttctccg ccgccatccg caaatagcgt cggccgggcc
     1801 gatgcccgtc atcgggccat gacaggtgtg tcctctgccc gccccgcata ttttctttga
     1861 tctgccggcg gctgcatgct ttctggcgat cctgcctttc tgacccgcat gtgtgtcttg
     1921 ccgatgtctg atcctcatag tctcgccccg cttctgacgc gcatcgccga ggcgctggag
     1981 cgtatcgcgc cccccgcccc ggcacggccg gactacgagg cggcggatgc ttttgtctgg
     2041 tcggccagtc gccaggcgct gacgccggtg gtccgcgtca accggattcc gctggagctg
     2101 ctggtcggga tcgatcgtat ccgcgacctc ttggtcgaca atactcggcg cttcgcccgc
     2161 ggtctgccgg ccaacaacgt gctgctgtgg ggcgcgcgcg gcatgggcaa gtcctcgctg
     2221 gtcaaggcgg cccatgccag cgtcaatgcc gaagttggcg caaccatgcg gctcaagctc
     2281 gtcgagatcc atcgcgagga catcgagacc ctgccagcgc tgctgacaac gatgcggacc
     2341 tcatcgcacc gttttctggt attctgcgac gatctgtcgt tcgatgccgg cgagacctcc
     2401 tacaagtcgc tgaaggcggt gctggaaggc gggctggaag gccgcccggc caatgtcctg
     2461 ttctatgcta catcgaaccg ccgccatctg atgccgcgcg acatgatcga caatgaacgc
     2521 gccaccgcaa tcaatcccgg cgaggcggtt gaagaaaagg tgtcgctgtc cgatcgcttc
     2581 gggctctggc tcggcatcca caagtgctcg caggaggaat atctggccat ggtgcatgcc
     2641 tatgcgaggc atttcgggct caagctgcgg cggacaagac ttgatgcgga cgcgctggaa
     2701 tgggccacga cccgcggctc gcgctccggc cggacggcct ggcagttcat ccaggatctc
     2761 gccggccgtc agggcaagct gctcgatctc tgagcgcttc gccaatcgca cgagacgccg
     2821 aattccacga cgggaagctg ggtccttgcc gaacgcttga caagaacaaa aaaagaacta
     2881 cgcataaaag cggggttcgg gaattgcgga caggtcagaa ccgagggcca ctcgtatcgc
     2941 ttgtggacaa cagcaagatt tcgcgggagc gggaccgcct gctccgcgta gaatggcgtc
     3001 tttcatatgc gccgggagac aggaatggcc ggaagtttga acaaggtgat gctgatcggc
     3061 aatctgggcg ccgaccccga gatccgcagg ctgcccagcg gcggctcggt cgccaatctc
     3121 aggatcgcga cctccgaaag ctggcgcgac aaggcaacgg gcgagcggcg cgaaaagacc
     3181 gagtggcaca atgtcgtggt cttcaatgac aatctggttc gcgtgatcga gcaatatgtc
     3241 aagaagggct ccaaggtctt tgtcgaaggc cagttgcaga cgcgcaagta tcaggacaag
     3301 tccggccagg accgctattc gaccgaggtg gtgctgcagg gcttttccgg caacctgacc
     3361 ttgctggatg ggcgcggcga cggcgcacgc gacggcggcg acggacgcgg ctccggtgat
     3421 ttcggccgat cttcgccgat ggatcgcaag ccgtccggcg gcgggcgctc cactgcgctc
     3481 gatgacgatg acattccgtt ttgaggcagg gccgcggagg cgacggacca ttgccgtcac
     3541 cgccatggcc tgaacgaggg tcgctgggag gtcgtaggga gatgctgttc agcataggca
     3601 agcgcttggc agaaatggcg atgatcgcgc tgatggccac cggggccatg gcgcaggaca
     3661 tgttggtgga gaaaaagacc ttcgagatgg cgtcgctgac gacggtgtcc ggagcgacgg
     3721 tgcgcaacgt caaagtcggc tgggaagcct atggcacgct caatgccgac aagagcaatg
     3781 tgattctcgt cccgcatttc ttttccggca ccagccacgc tgccggaaag tacaagccgg
     3841 acgacaagct ccccggctac tgggactatc tgatcggccc cggaaaggcg gtcgatacca
     3901 acaggttcta tgtcatttcg gtcgattccc tggtcaatct caacgccaag gatccgaatg
     3961 tcgtcaccac cgggccggcc tccatcaatc ccgataccgg caagccctac gggatgacct
     4021 tcccgctcgt gaccattcgc gatttcgtca atgtccagaa ggcgttgctc gatagcctcg
     4081 gcatccgcaa gctgcatgcc gtgatgggcg cgtcgatggg cggcctgcaa tcctatgaat
     4141 gggcggcgag ctatcccgac atggtcgaaa ggctgctgcc ggtgatcgct gcgggcgacc
     4201 cggggccatg gctgaacgcc tggctcaatg tctgggcctc tcccatcctg atcgatgcga
     4261 actggaacaa gggggactat tacggcaagg ccgagccggt cgaggggctc aaaaccgcac
     4321 tcaagatcgt ctcccttcat gccaaccatt ggctgtgggc ggaaaagacg atcggcgccg
     4381 gctgggcgac cgagggcaag gacccgctcg ccgctttcga caaccggtac aagatcgagg
     4441 cgacactcga cgccgccggg gccgcccgtg cggccgtgtc ggatgccaat cacttcctct
     4501 atctggtcaa ggccaaccag accttcgtgc ccggcggcgc caagacggcg gcggaagggt
     4561 tgaagaagat caaggcgcgc acgctgatgc tctatgcacc gaccgatcag gtctttgccg
     4621 ccgattgggt ccgggcgacg gcggaggcga tccgcgccaa cggaacgccg gtggaaaccg
     4681 ccgagatcgc cggcaatttc ggccacctca acggcgtgct ggcgctcaag ccgcttgaag
     4741 ccaggattgt ggatttcctg acaaaatagc gggcatgggc caccgtgcgg cggtgtgaac
     4801 cggcgatgca ggtcgccgtt tccgctggtt cgatgacgaa cgc
//
LOCUS       DAOMXG010000178         4778 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_9520_length_4778_cov_9.235655, whole genome shotgun sequence.
ACCESSION   DAOMXG010000178 DAOMXG010000000
VERSION     DAOMXG010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4778
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9520_length_4778_cov_9.235655"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="PLQ11_10670"
     CDS             <1..797
                     /locus_tag="PLQ11_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25405.1"
                     /translation="IAAALDHIRKTPAASAAPSLNRPIIAAALSASLGLAEASVRPLI
                     ALLRRLDADAAREDARRITEEITAAGAAVVMDETGQVAPDTRIDTLDSWAQSIDAGFD
                     VEESMAGVDHWEDVPAEDLAAEDPSETGHASHEVEDLPPPAAMDMEPVPSPALARLLM
                     LQGRLNASIAEPSPVEAAVAADEPATEPVNAVTDEAPPLDPDEAYRADLDSLFAATFG
                     DAAEAATVEDDVNPPSAEALRVINSLHDALDAGFFNNWDIRRRDAA"
     gene            complement(813..1586)
                     /locus_tag="PLQ11_10675"
     CDS             complement(813..1586)
                     /locus_tag="PLQ11_10675"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012496806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HRK25406.1"
                     /translation="MPQASRSIAFIASSAPEAQSARERLARYYGDADPETADVVVALG
                     GDGLMLQVLHRFMGRHKPVYGMNRGSVGFLMNEFSEMGLRERLAAAERSTIHPLLMKA
                     EDADGAVHRSHAVNEVSLLRQSYQAAKLAIAVDGKERMQELICDGVIVATPAGSTAYN
                     YSANGPILPLNAPLLALTPISAFRPRRWRGALLPDHAQVSITVLEADKRPVSAVADHF
                     ETRNVRHVHVAIDRSIDMLMLHDPGHSLDERILREQFGY"
     gene            complement(1869..2828)
                     /locus_tag="PLQ11_10680"
     CDS             complement(1869..2828)
                     /locus_tag="PLQ11_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRK25407.1"
                     /translation="MTLSRRTVLACAAATAGLAALPAHAKAPLAGTQAPGFFRMKLGE
                     MEVTTINDGFFVRPLAGFVKNAMDEEVAAAIQDARLPGNGVFIPFTTVVINTGDKLVL
                     IDCGNGDSGAPTSGRWMTNFKAAGFDPKDVDTVIISHFHGDHINGVRLKDGTAVFPNA
                     EVMVPSAEWAFWMDDAKMSAAPDPMKPAFQNVRRVFGPQAKDVKQFEPGKEIAPGITS
                     IAAGGHTPGHNVFMVASGNAKLMCLADTTNNPYLFARHPDWQAVFDMDGNAAAATRKK
                     LLDMAAEEEAQVHMYHAPFPATGFIEKAGNGYDFVPVVWSSAL"
     gene            complement(2940..3818)
                     /gene="folD"
                     /locus_tag="PLQ11_10685"
     CDS             complement(2940..3818)
                     /gene="folD"
                     /locus_tag="PLQ11_10685"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HRK25408.1"
                     /translation="MTATLIDGKAVAARTRAETAALVAEIRERHGIVPGLHVVLVGDD
                     PASQVYVGSKEKMAAKAGFNSVVHRLPATTSQTELLALIDRLNRDEAVDGILVQLPLP
                     RGLDANAVIDAIDPDKDVDGFHLTNVGKLSAGRAALVPCTPQGSMILIRSVTTALSGF
                     NAVVIGRSAIVGKPMAQLLLNADCTVTIAHSRTRDLPSVCRGADVLIAAVGRPEFVRG
                     DWIKPGAIVIDVGINRLADGRLVGDVAFDEAVAVAGAITPVPGGVGPMTIACLLQNTA
                     IAALTRRGLALPAVLA"
     gene            complement(3837..4700)
                     /gene="purU"
                     /locus_tag="PLQ11_10690"
     CDS             complement(3837..4700)
                     /gene="purU"
                     /locus_tag="PLQ11_10690"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018246141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HRK25409.1"
                     /translation="MTSASASPLILTLSCKDVIGIVAEVAGFLAGHGCNILDSKQYFD
                     PDTGLFFMRVVVASERGASAEALRAAFAPVGQRFAMDWALHDPSVKPRVLLMVSKFGH
                     CLNDLLYRWTIGALPVEIAAVVSNHRDFYRAVAAHGIDFHHIPVTPETKREAEQRLMD
                     IVEQTRSELVVLARYMQVLSDDLCTRLSGRAINIHHSFLPSFKGARPYHQAHRRGVKL
                     IGATAHYVTADLDEGPIIDQEVARVSHAQSPDDLVAIGRDIESVVLARAVLAHVERRV
                     FLNGSKTVVFS"
BASE COUNT          874 a         1582 c         1523 g          799 t
ORIGIN      
        1 ccattgccgc agcgctggat cacatccgca agacgcccgc cgcttccgct gcgcccagtc
       61 tcaatcgtcc catcattgcg gcggccctgt ctgccagcct ggggctcgca gaggccagcg
      121 tccgcccgct gatcgccttg ctgcggcggc tcgatgccga tgcagcccgc gaggacgccc
      181 ggcgcatcac cgaggaaatc accgcagccg gcgcggccgt tgtgatggac gaaaccgggc
      241 aggtggctcc ggacacccgc atcgatacgc tggattcctg ggcccaatcc atcgacgccg
      301 ggttcgatgt cgaagaatcc atggccggtg ttgatcactg ggaggatgtc ccggccgagg
      361 accttgctgc cgaggacccg tctgagacgg gccatgcctc ccacgaggtc gaggaccttc
      421 cgccgccggc agcgatggac atggagccgg ttccctcgcc tgcgctggcg cgtctcctga
      481 tgctacaggg ccggctgaac gcatcgattg ccgagcctag cccggtcgaa gccgccgtcg
      541 ccgccgatga gcccgccaca gagccggtca atgcggtgac agacgaggcc ccgccgctgg
      601 acccggacga agcctatcgc gccgacctcg acagcctctt tgccgccacg tttggcgatg
      661 cagccgaggc ggccaccgta gaggacgatg tgaacccgcc atcggccgaa gcgctacgcg
      721 tgatcaattc cctgcacgac gcgctcgatg ccggcttctt caacaactgg gacatccgcc
      781 gccgcgacgc cgcatagcgg caccggaacc gatcaatagc cgaactgctc ccgcaggatc
      841 cgctcgtcca ggctatggcc gggatcatgc agcatcagca tgtcgatcga tcggtcgatg
      901 gcgacatgga cgtggcggac attgcgcgtt tcaaaatggt cggcgactgc cgagaccggg
      961 cgcttgtccg cttccaatac ggtgatgctc acctgcgcat ggtcgggcag caacgcaccg
     1021 cgccagcgac gcggccggaa cgcggaaatc ggtgtcagcg ccagcagcgg cgcattgagc
     1081 ggcaggatcg gcccgttggc ggaataattg taggccgtcg agccggccgg tgtcgccacg
     1141 atcaccccgt cgcagatgag ttcctgcatc cgctccttgc catccacggc gatggccagc
     1201 ttggccgcct gataggattg gcgcagcagc gagacttcgt tgaccgcatg cgagcggtgg
     1261 accgccccgt ccgcatcctc ggccttcatc agcagcggat ggatggtgct gcgctcggca
     1321 gcggccagcc gctcgcgcag gcccatctcg ctgaattcat tcatcaggaa gccgaccgag
     1381 ccacggttca tgccatagac cggcttgtgc cgtcccatga agcgatgcag cacctgcagc
     1441 atcagcccgt cgccaccgag ggcgaccacc acatcggcgg tttccgggtc ggcatcgcca
     1501 taatagcggg ccagccgctc gcgcgcgctc tgcgcttccg gcgcgctgga ggcgatgaag
     1561 gcgatgctcc tactggcctg cggcacgcgc gactcccatg ctttggtcca tcatagagca
     1621 agccggggaa aaggggaggg cgtggcgcgc gcgccccgcc atccggtgac ccgtcagcat
     1681 gccttgccgc gcggcttcct gcgggcctgc ctggtatgcc cgcaggggcg agaacacggg
     1741 cgttatacca aaaagtcatt taaaatgact ctttggagca actcaaaaat ttctcatctc
     1801 gttgataaaa gtgagaaatt tttgaaacga accaagtcgc ggcgagttcc tcgccttgcg
     1861 acttggcatt acagcgccga cgaccacacc accggcacga aatcatagcc gttgccggcc
     1921 ttttcgatga acccggtggc cgggaacggc gcgtgataca tatgcacctg ggcctcttcc
     1981 tccgccgcca tgtcgagcag cttcttgcgc gtggcggcgg cggcattgcc atccatgtcg
     2041 aacaccgcct gccagtccgg atgccgggcg aacagatagg ggttgttggt ggtatcggcg
     2101 aggcacatca gcttggcatt gccgctggcc accatgaaca cattgtggcc cggcgtatga
     2161 ccgcccgctg cgatggaggt gatgcccggc gcgatctcct tgcccggctc gaactgcttc
     2221 acatccttgg cctgcgggcc gaacacgcgg cgcacattct ggaatgccgg cttcatcgga
     2281 tcgggggccg cgctcatctt ggcgtcatcc atccagaatg cccattcggc ggagggcacc
     2341 atcacttcgg cattggggaa aacggcagtt ccgtccttga gccgcacgcc gttgatgtga
     2401 tcgccgtgga aatggctgat gatgaccgta tcgacatcct tgggatcaaa gccggccgcc
     2461 ttgaaattgg tcatccagcg gccggaggtc ggtgcgccgg aatcgccgtt gccgcaatca
     2521 atcagcacca gcttgtcgcc ggtattgatg acgaccgtgg tgaatggaat gaacacgccg
     2581 tttccgggca ggcgggcatc ctggatggcg gcagccacct cctcgtccat ggcattcttg
     2641 acgaagccgg ccagcggccg gacgaaaaac ccgtcgttga tggtggtaac ctccatctcg
     2701 cccagcttca tgcggaaaaa gcccggcgcc tgggtgccgg ccagcggcgc cttggcatgg
     2761 gcaggcaggg cggcaaggcc ggcggttgcg gctgcacagg ccagaacggt tctgcgcgaa
     2821 agggtcatga cagtctcctt acctgattgt cgggcgcctg agcggcacag gtcgaacctg
     2881 gccgcagtct gcatgatttg gcggcgaagt gcagtcaaat ttcggtgtat gccgccgggt
     2941 caggcgagca cggccggcag cgccagcccg cgccgggtca gcgcggcaat ggcggtgttc
     3001 tgcaagaggc aggcgatggt catcggcccg acaccgccgg gaaccggcgt gatcgccccg
     3061 gccacggcca ccgcctcgtc gaaggccaca tcgcccacca gccggccatc cgccagccgg
     3121 ttgatgccga catcgatgac gatggccccc ggcttgatcc agtcgccgcg cacgaattcg
     3181 ggccgcccga cggcagcgat caggacatcg gcaccgcggc acacggacgg caggtcgcgc
     3241 gtccgggaat gggcgatggt gaccgtgcaa tcggcgttca gcagcaattg cgccatcggc
     3301 ttgccgacaa tcgccgaccg gccgatcacc accgcgttga agccggagag cgctgtcgtc
     3361 accgatcgga tcaggatcat cgagccctgc ggcgtgcagg gcaccagcgc ggcgcggccg
     3421 gccgaaagct tgccgacatt ggtgaggtga aagccgtcga catccttgtc cgggtcgatg
     3481 gcgtcaatga ccgcattggc gtcgagcccg cgcggcagcg gcagttgcac cagaatgcca
     3541 tccaccgcct catcgcggtt gagccggtcg atcagggcca gcaattcggt ctgcgatgtc
     3601 gtggccggca ggcgatgcac caccgaattg aacccggcct tggccgccat cttttccttg
     3661 gagccgacat agacctggct cgccggatca tcgccgacaa gcacgacatg cagccccgga
     3721 acgatgccgt ggcgctccct gatctcggcg acgagcgcag cggtctcagc gcgggtgcgg
     3781 gcggcaacgg ccttgccgtc gatcagcgtt gcagtcacgt ccacgcgctc cccactttac
     3841 gaaaaaacga ccgtcttgct gccgttgaga aagacgcgcc gttcgacatg cgccagcacg
     3901 gcccgcgcca gcaccacgga ttcgatgtcg cggccgatgg cgaccagatc atcgggcgac
     3961 tgggcatggc tgacgcgcgc cacttcctga tcgatgatcg gcccctcatc gagatcggcg
     4021 gtgacgtaat gggcggtggc gccgatcagc ttgacgccgc gccggtgcgc ctggtggtag
     4081 gggcgggcgc ccttgaagct cggcaggaac gagtgatgga tattgatcgc ccggcccgac
     4141 aggcgcgtgc acaggtcatc cgacagcacc tgcatgtagc gcgccagcac gaccagttcc
     4201 gatcgcgtct gctcgacgat gtccatcagc cgctgctcgg cctcgcgctt ggtctccggc
     4261 gttaccggaa tatggtgaaa gtcgatgcca tgcgccgcaa ccgcgcggta gaaatcacgg
     4321 tggttcgaca caacggcggc gatctcgacc ggcagcgccc cgatggtcca gcgatagaga
     4381 aggtcgttga ggcaatggcc gaacttcgac accatcagca gcacgcgcgg cttgaccgac
     4441 ggatcatgca gcgcccagtc catcgcaaac cgctggccca ccggcgcaaa ggctgccctc
     4501 agcgcctcag ccgaggcccc gcgctccgat gccaccacga cgcgcatgaa gaacagcccg
     4561 gtgtctggat caaaatactg ttttgaatca aggatgttgc agccatggcc ggcaagaaag
     4621 ccggccacct cggcgacaat cccgatcaca tccttgcacg acagagtcag gatgagggga
     4681 gaggcggagg cgctggtcat catcatgctt tctggtgccg gcagagggga ttgaaccccc
     4741 gaccttcggt ttacaaaacc gctgctctac cgctgagc
//
LOCUS       DAOMXG010000179         4737 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_9635_length_4737_cov_9.109355, whole genome shotgun sequence.
ACCESSION   DAOMXG010000179 DAOMXG010000000
VERSION     DAOMXG010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4737
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9635_length_4737_cov_9.109355"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1833)
                     /gene="pheT"
                     /locus_tag="PLQ11_10695"
     CDS             complement(<1..1833)
                     /gene="pheT"
                     /locus_tag="PLQ11_10695"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012453516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRK25410.1"
                     /translation="MKVTLSWLKDHLDTNASLDEVVTTLTRIGLEVEGVENPAEKLKG
                     FSIAYVTEAKQHPNADRLRVCMVDTGSGEPVQVVCGAPNARTGMKGVFSPAGTFIPGK
                     NITLGKGVIRGVESNGMLCSGAELMLSEDHDGILDLPADAPVGVDFATYLGLDDPVIE
                     INLTPNRPDATGVYGIARDLAAAGLGTLKTPKPPTFAAQGPCPVDVTLDFADADRHLC
                     PAFALRVVRGVKNGPSPEWMQKRLKAIGLRPINALVDITNFMTFDQGRPLHVFDLKKV
                     AGGLVVRRAKAGESLLALDGRSYSLDPSMVVIADEKGVESLAGIMGGEHSGCDGATSD
                     VLIESALWDPLTVARTGRALAINTDARYRFERGVDPAYTVPGLDRATALVIDLAGGAA
                     SEAVVAGAIPEATRVIDFPWDETRRLTGLDVPRPEAKVILQTLGFWVSGKGDVVKVAP
                     PSWRPDIEGKADLVEEITRIIGVDNVPMVPLQREDKVHAPVLTLIQKRTRLARRLLAA
                     RGMMEAVTWSFISKEAAGLFGGGAAALALANPIASDLSDMRPTLLAGLIAASQRNADR
                     GQHDLALFEVGQIFRGDAPQDQKIAAAGLRRGIAKAGGAGRHWSG"
     gene            complement(1927..2301)
                     /locus_tag="PLQ11_10700"
     CDS             complement(1927..2301)
                     /locus_tag="PLQ11_10700"
                     /inference="COORDINATES: protein motif:HMM:NF039367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6173 family protein"
                     /protein_id="HRK25411.1"
                     /translation="MTGEPAPRTDFSQADWMFERIIKSLQAFEAALAADEEIGVRLAA
                     FSDEVLSIESVGYWNPDLIKFTGVTGNGERYELVQHVSQVSLLMAARPKRGGEARRIG
                     FILGEQLEKARDKQAVNATAVV"
     gene            complement(2306..2434)
                     /locus_tag="PLQ11_10705"
     CDS             complement(2306..2434)
                     /locus_tag="PLQ11_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25412.1"
                     /translation="MRRLKIGEFRAIYVIDGDIIQVRLIGKRNDDDVYRALERMTR"
     gene            complement(2532..2768)
                     /locus_tag="PLQ11_10710"
     CDS             complement(2532..2768)
                     /locus_tag="PLQ11_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004121362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HRK25413.1"
                     /translation="MKTVTAAEAKNGFGKFLDMAQREPVLVTKNNRPVGVFLSMADIE
                     DTIWAERAQKAHAEGYLGPGDSRSLLGALVHAKD"
     gene            complement(2795..3256)
                     /locus_tag="PLQ11_10715"
     CDS             complement(2795..3256)
                     /locus_tag="PLQ11_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25414.1"
                     /translation="MTIDAALLSLLSAILGGLIVAAINHVFSRRRELDAKKRDIALSK
                     LLEAWLALDAASHKPGVYDGEIHKLEEATRLILLFAEDDDIDKIETITKNVTKKEPAD
                     YTPLLKSLRQKIRDQIGLEGRARHFWFAAKPIQTPTPKDHKKDNRHLDQIG"
     gene            complement(3267..4349)
                     /gene="pheS"
                     /locus_tag="PLQ11_10720"
     CDS             complement(3267..4349)
                     /gene="pheS"
                     /locus_tag="PLQ11_10720"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003610093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HRK25415.1"
                     /translation="MTDIASLQSSILAEIAAAADEAALEGVRVAALGKKGSISALLGT
                     LGKMSPDERRTAGAAINAVKDAVTDALAQRKIVLKAAALDARLKSETVDVTLPVRAST
                     LETGRIHPISQVIDELTAIFADLGFAVAEGPDIETDDYNFTKLNFPVGHPAREMHDTF
                     FFAPDAAGERKLLRTHTSPVQIRTMLQQKPPIRVICPGRTYRCDSDQTHTPMFHQVEG
                     LVIDKSANLGQLKWVLAEFCKAFFEVPNVTMRFRPSFFPFTEPSMEVDIQCRRQGGDI
                     RFGEGEDWLEILGCGMVHPNVLRNCGIDPDVYQGYAWGMGIDRIAMLKYGMADLRPFF
                     EADVRWLSHYGFRPLDLPSLAGGLTG"
BASE COUNT          840 a         1511 c         1469 g          917 t
ORIGIN      
        1 gcccgaccag tgccggccgg cgccacccgc cttggcgatg ccccgcctca ggccggcggc
       61 ggcgatcttc tgatcctgag gcgcatcgcc gcggaaaatc tggcccacct cgaacagggc
      121 gagatcatgc tgcccgcggt cggcattgcg ctggcttgcg gcgatcagcc cggcgagcag
      181 ggtcgggcgc atgtcggaga ggtccgaggc aatcggattg gcgagcgcca gcgccgctgc
      241 accgccgccg aacaggccgg ctgcctcctt cgagatgaac gaccaggtaa cggcttccat
      301 catgccgcgc gccgccagca gccggcgggc caggcgcgtc cgcttctgga tcagcgtgag
      361 aacgggcgca tgcaccttgt cctcgcgctg gagcggtacc atcggcacat tgtcgacgcc
      421 gatgatgcgg gtgatttcct cgacgagatc ggccttgccc tcgatgtcgg gacgccacga
      481 gggcggcgcc accttgacca cgtcgccctt gcctgacacc cagaagccga gcgtctgcag
      541 gatcactttt gcttccggac gtggcacgtc gaggccggtg agccgcctcg tctcgtccca
      601 cggaaaatcg atgaccctgg tcgcctcggg aatggcgccg gccaccaccg cctcgctggc
      661 ggcgccgcca gcgaggtcga tcaccaatgc ggtggcccgg tcgagcccgg gcacggtata
      721 ggcaggatcg acgccgcgct cgaaccggta gcgggcatcg gtgttgattg cgagcgcgcg
      781 gccggtgcgg gcgacggtga gcggatccca cagcgccgat tcgatcagca catcactcgt
      841 cgcgccatcg cagccggaat gctcgccgcc catgatgccg gcgagtgact cgacgccttt
      901 ctcgtcggca atcaccacca tcgacggatc gaggctgtag ctgcggccat cgagcgccag
      961 caggctttcg ccggccttgg cccgccgcac caccagtccg cccgcgacct tcttgaggtc
     1021 gaacacatgc agcggccggc cctgatcgaa ggtcatgaaa ttggtgatgt cgaccagcgc
     1081 attgatgggc ctgagaccga tggctttgag ccgcttctgc atccactcgg gcgaggggcc
     1141 gttcttcacc ccgcgtacca cccgcagcgc gaaggcgggg cagagatgcc ggtccgcatc
     1201 ggcaaaatcg agcgtgacgt cgaccgggca ggggccctgc gccgcgaagg tcggcggctt
     1261 tggtgtcttg agtgttccca ggccagcagc agcgagatcg cgcgcgatcc cgtagacgcc
     1321 ggtcgcatcg gggcggttcg gcgtcaggtt gatctcgatc accggatcat cgaggccgag
     1381 ataggtggca aaatccaccc ccaccggcgc atcagccggc aggtcgagga tgccgtcatg
     1441 gtcctcgctc agcatcaact cggcgcccga gcacagcatg ccgttggatt cgaccccgcg
     1501 gatgacgccc ttgcccaagg tgatgttctt gcccggaatg aacgtgcccg ccggcgagaa
     1561 cacgcccttc atgccggtgc gcgcgttggg agcgccgcac accacctgca ccggttcgcc
     1621 gctgccggta tcgaccatgc agacacgcag ccggtccgca ttgggatgct gctttgcctc
     1681 tgtcacatag gcgatggaaa agcccttcag cttttcggcc gggttctcga ctccttcgac
     1741 ctccagcccg atgcgggtca gcgtcgttac cacctcatcg aggctggcgt tggtatcgag
     1801 atggtccttg agccaggaca gggtgacttt catggggctc gttcctcgcg gaaaggcaat
     1861 gcggcgtgat cctcctaaac gcatgctgcg cttgagaaaa ggggcaaatt ccgcactgcg
     1921 gcgaaatcaa accaccgccg tggcgttgac cgcctgcttg tcgcgcgcct tttcgagctg
     1981 ttcgccgagg atgaaaccga tccgccgcgc ctcgccgccg cgctttggcc gtgcggccat
     2041 cagcaggctg acctggctga catgctggac aagctcatag cgttcgccat tgccggtgac
     2101 gccggtgaac ttgatcaggt ccgggttcca gtatccgacg ctttcgatcg acagcacctc
     2161 atcggaaaag gccgccagcc gcacgccgat ttcctcatcc gccgcgagcg cggcctcaaa
     2221 cgcctgcaac gatttgatga tccgctcgaa catccagtcg gcctgggaaa agtcggtgcg
     2281 gggggcaggt tcgccggtca tggggtcacc tcgtcattcg ttcgagcgcg cggtagacgt
     2341 catcatcatt tcgcttgccg atcaggcgca cctgaatgat gtcgccgtca atcacataga
     2401 tggcgcggaa ttccccgatt ttcagccgcc gcattggcgc gtagccctag agaaactgcg
     2461 aattcggcgg ttccggctgg ctttccagag ccagaagccg ttcggcgacc tgtcgggcct
     2521 gtttggtcag gtcaatcctt ggcatggacc agcgcgccga ggaggctgcg gctgtcgccc
     2581 ggcccgagat agccctcggc atgggccttc tgggcacgct cggcccagat cgtgtcctcg
     2641 atatcggcca tcgacaggaa cacgccgacc ggccggttgt tcttggtcac cagcaccggc
     2701 tcgcgctgcg ccatatcgag aaatttgccg aagccattct tggcctcggc agcggtaacg
     2761 gttttcatgg gatgcgctcc aagtattagc tattttagcc aatttggtct aaatgtctat
     2821 tatctttttt gtgatccttg ggagttggag tttgaatcgg cttagcggca aaccaaaagt
     2881 ggcgcgctct tccttcgagg ccaatttgat ctcgtatttt ttggcgcaat gatttcagta
     2941 acggcgtgta atctgctggc tcctttttcg ttacgttctt tgtgattgtt tcaattttat
     3001 caatatcgtc atcttccgca aacagcagaa tcagccttgt tgcttcttcc aatttgtgaa
     3061 tctcgccatc ataaacgcca ggtttgtgcg aggctgcatc caatgccaac caagcctcaa
     3121 gcagctttga gagcgcaata tcccgctttt tggcatctaa ttcacgacga cgactgaaca
     3181 catgatttat ggcagcaaca atcaagccgc ccagaatggc tgacaacagc gaaagcaggg
     3241 cagcgtcaat tgtcattgac cgcccctcac cccgtcaacc cgcccgccag gcttggcaga
     3301 tcgagcgggc ggaagccgta gtggctcagc cagcgcacat cggcttcgaa gaagggcctg
     3361 aggtcggcca tgccgtattt cagcatggcg atgcgatcga tgcccatgcc ccaggcatag
     3421 ccctgataga catcaggatc gatgccgcaa ttcctgagca cattgggatg aaccatgccg
     3481 cagccgagga tttccagcca gtcctcgccc tcgccgaagc ggatgtcgcc accctggcgc
     3541 cggcactgga tgtccacttc catcgacggt tccgtgaacg ggaagaacga cgggcggaag
     3601 cgcatggtga cgttcggcac ctcgaagaat gccttgcaga actccgccag cacccatttg
     3661 agctggccga ggttggcgct tttgtcgatc accagaccct cgacctgatg gaacatcggc
     3721 gtatgggtct ggtcggaatc gcagcgatag gtgcgaccgg ggcatatgac gcggatcggc
     3781 ggcttctgct ggagcatcgt gcggatctgc accggcgagg tatgggtgcg cagcagcttg
     3841 cgctcgcccg ccgcatcggg cgcgaagaag aacgtgtcgt gcatctcccg cgccggatgt
     3901 ccgaccggga aattcagttt ggtgaaattg taatcgtcgg tctcgatgtc cggcccttcg
     3961 gcgacggcaa agccgagatc ggcgaagatg gctgtcaatt catcgatgac ctgcgaaatg
     4021 ggatgaatgc ggccggtttc cagcgtgctg gcgcgcaccg gcagggtcac gtccaccgtc
     4081 tccgatttca gccgcgcatc gagcgctgcg gccttcagca caatcttgcg ctgggcgagc
     4141 gcgtcggtca ccgcatcctt gaccgcgttg atggcggcgc cggcggtcct gcgttcgtcg
     4201 ggcgacatct tgcccagagt tcccagcagg gccgaaatgg accccttctt gccgagcgcg
     4261 gcgacgcgca cgccctccag cgccgcctcg tcggcggcgg cggcaatctc agcgaggatc
     4321 gaactctgaa gcgaggctat gtcggtcatg gcggcaacaa cggctgaaga acagggctgt
     4381 ccggctctta gagcagtttg ttttcatacg gaagcaattc tgtttcgcgc gcggcgtgac
     4441 gatccgggcg cgaggcggcg gacaggcggc ccaatgcctg ccacaatccc ggcgttcctt
     4501 tccggcagga ctctggcgcg cgctcgaacg tcgcgctagt cgttgtgaga accgggaatc
     4561 atccgctgtc catgaagcct tcgctcctca ttctcattgc cgttgcggcg cttgcctgcg
     4621 ctggcggcag cgcgccggca ttggccagga ccgccaagga ctgcgaggcc atccagaacc
     4681 cgatggagtt caaccggtgc ctcgcctcgc tgtcgccggt gcgcggctcg cgcgcgg
//
LOCUS       DAOMXG010000180         4711 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_9725_length_4711_cov_7.815722, whole genome shotgun sequence.
ACCESSION   DAOMXG010000180 DAOMXG010000000
VERSION     DAOMXG010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9725_length_4711_cov_7.815722"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..1836
                     /locus_tag="PLQ11_10725"
     CDS             79..1836
                     /locus_tag="PLQ11_10725"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HRK25416.1"
                     /translation="MTTTSSSRRRDTLTLALAQLNPTVGDIAGNAEKLRTARATAARA
                     GADIVMFTELFLAGYPPEDLVLKPAFVDACRAMLETLARETADGGPAVLVGLPWAEGD
                     GLFNAYAALDAGRIVGVRFKADLPNYGVFDEKRVFNVGPMPGPLALRGLRLGLPICED
                     IWGSEVVECLAETGAEILLVPNGSPYWRGKTDERMNVAAARVTESGLPLVYLNQICGQ
                     DELVFDGASFGLNADCALAFQMPAFVEDVAITHWSRIGSGWVCTEGPMALVEEGEKAD
                     YAACMLGLRDYVDKNRFPGVVLGLSGGIDSALCAAMAVDALGPDRVHCIMMPFRFTSS
                     DSLADAKACAAALGVRYDSLPIASAVEGFEAILSPLFAGSARDITEENLQSRARGTLL
                     MAVSNKFGPMVVTTGNKSEMSVGYATIYGDMNGGFNPIKDLYKMEVYRLSALRNAWKP
                     EGAKGPGGIVIAENILVKAPSAELRDNQKDQDSLPPYEVLDAILEMLVEKEMRISEVV
                     AAGHDLETVRKIERLLFIAEYKRRQAAPGVKVTRKNFGRDRRYPIVNRFRDPGLGPHR
                     PDPELTPEVVTGTAAILDV"
     gene            1845..3176
                     /gene="gltX"
                     /locus_tag="PLQ11_10730"
     CDS             1845..3176
                     /gene="gltX"
                     /locus_tag="PLQ11_10730"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012322451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HRK25417.1"
                     /translation="MMIVRFAPSPTGYLHIGNARTALWNALHARRHGGQFILRLDDTD
                     VERSQEIYAEAIQQDLAWLGIRWDRLERQSLRLDRYGMALERLKAAGLVYPAYETPDE
                     LERKRKRQQARGLPPVYDRASLKLTEAERTALEARGRHPHWRFRLSGATVEWQDGVRG
                     PCHIDTGSLSDPVLVREDGTVLYTFASVVDDADMGVTDIIRGEDHVANTAVQIELFHA
                     LGVAPPRFAHHNLIVAASGEAMSKRTGALSIRSFRKGGADPMAVAAVAVLTGTSEPVR
                     PLASLDDLVPLAELSGISRALTKFDPAEIGHLTTRLLHTKPYAAVRDRLAAAGIDGAR
                     AELFWDAVRANLADMEEAVSWWHVVNGPIAPHVRSDPELIETARRLLPPEPWDETTWG
                     IWCAAVTAATGARGKALFLPLRLALTGLDHGPELKRLLPLIGRAEAERRLV"
     gene            complement(3139..4149)
                     /locus_tag="PLQ11_10735"
     CDS             complement(3139..4149)
                     /locus_tag="PLQ11_10735"
                     /inference="COORDINATES: protein motif:HMM:NF022515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2865 domain-containing protein"
                     /protein_id="HRK25418.1"
                     /translation="MSKLRLRIPAMMLALVVSQAAGAQTGSSGSPGFLANLLGIRPAT
                     TEAPMNLAAPPPAGKAARTSQQSASPAPAKAGTAKAVAAKADTAAPPVPRSAGKAADT
                     RTAAAGTIDPKPSDPGADLDRPVRKPVKLPPFRGGGSQAVCVRTCDGFFFPVNYEGAH
                     GSDRYAEACQLACPSAPTEVYFMPRGGDLKQAANQYGQRYAALPNAFKYRKERDASCA
                     CKAQNQSWGEVLAHVEPMIKQGKDDILVTPERSLELARPADPAEKIVAEAAAPRKAAK
                     SKKAGKPAIADAKPALRTSIKPDQAATRPAPARPVASRQPIDPETTASIRRAAVPLPP
                     AR"
     gene            complement(4280..>4711)
                     /locus_tag="PLQ11_10740"
     CDS             complement(4280..>4711)
                     /locus_tag="PLQ11_10740"
                     /inference="COORDINATES: protein motif:HMM:NF022515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2865 domain-containing protein"
                     /protein_id="HRK25419.1"
                     /translation="ARAEDGQRYTALPNAFQYRKAYDPSCSCKQPQQSWASALRDADK
                     LVRDRANDITVTEERSNQMARPQQVRLRGVKGKTAPEEPVEMQLNTNFATNSTATAGI
                     GPAVSGNRMLGTGDGETREVNSKGGEKKQVRVLTPALSLSQ"
BASE COUNT          746 a         1503 c         1595 g          867 t
ORIGIN      
        1 agcctccgcc cggatcatcc cacggcccga gaaacgcaac tggttcaatc tggtcagaaa
       61 atgctctaaa tcagggccat gacaacgaca tcttccagcc gccgccgcga caccctcacc
      121 ctcgcgcttg cccagttgaa cccgaccgtt ggtgacatcg ccggcaatgc cgagaagctg
      181 cgcaccgccc gtgccacagc ggcgcgggca ggggccgata tcgtgatgtt caccgagttg
      241 tttctcgccg gctatccgcc ggaggatctg gtgctcaagc cggccttcgt cgatgcctgt
      301 cgggcgatgc tggaaacgct ggcgcgcgaa accgccgatg gcggtccggc ggtgctggta
      361 gggctgccct gggcggaggg cgacggattg ttcaacgcct atgccgcgct ggatgcgggt
      421 cgcatcgttg gcgtgcgttt caaggccgat ctgcccaatt atggcgtatt tgacgagaaa
      481 cgtgtgttca acgtcggccc gatgccgggg ccgctggcct tgcgcgggct gcgcctcggc
      541 ctgccgatct gcgaggatat ctggggcagt gaggtggtcg aatgcctggc cgaaaccggc
      601 gcggaaatcc tgctggtgcc caacggttcg ccctactggc gcggcaagac cgatgagcgc
      661 atgaatgttg ccgccgcgcg cgttaccgaa agcggcctgc cgctggtcta tctcaaccag
      721 atctgcgggc aggacgaact ggtgttcgac ggcgcatcct tcggccttaa tgccgattgc
      781 gcgctggcct tccagatgcc ggcctttgtc gaggatgtgg cgatcacgca ctggagccgc
      841 atcggctcgg gctgggtctg caccgagggg ccgatggcgc tggtcgagga gggcgagaag
      901 gccgactatg ccgcctgcat gctgggactg cgcgattatg tcgacaagaa ccgtttcccc
      961 ggcgtggtgc tggggctttc cggcggcatt gattcggctt tgtgcgcggc gatggcggtt
     1021 gatgcgctcg gtccggaccg ggtgcattgc atcatgatgc cgttccgctt cacctcatcg
     1081 gacagcctgg ccgatgccaa agcctgcgcc gcggcgctgg gagtgcgcta cgacagcctg
     1141 ccgatcgctt ccgcagtgga aggattcgag gcgatcctca gcccgctgtt cgccggcagt
     1201 gcccgcgata tcaccgagga aaacctgcaa agccgggcgc gcggcacctt gctgatggcg
     1261 gtttccaaca aattcggccc gatggtcgtg accaccggca acaagagcga gatgtcggtt
     1321 ggctatgcca ccatctacgg cgacatgaac ggcggtttca acccgatcaa ggacctctac
     1381 aagatggagg tctaccgcct ctcggccctg cgcaacgcct ggaagccgga gggcgccaag
     1441 gggccgggcg ggatcgtcat tgccgaaaac atcctggtca aggcgccgag tgccgaattg
     1501 cgcgacaacc agaaggatca ggattcgctg ccgccttacg aggtgctcga tgccatcctc
     1561 gaaatgctgg tggaaaagga gatgcggatc agcgaggtgg tcgccgccgg ccatgacctc
     1621 gaaaccgtgc gcaagatcga gcgcctgctc ttcatcgccg aatacaagcg acggcaggca
     1681 gcgcccggcg tcaaggtgac gcgcaagaat ttcggtcgtg accgccgcta tccgatcgtc
     1741 aaccgcttcc gcgatccggg cctgggtccg cacaggcccg atccggaact gaccccggag
     1801 gtcgtgaccg gcacggccgc catcctggat gtgtgaggct ggccatgatg atcgtccgct
     1861 tcgctccctc gccgaccggc tacctgcata tcggcaatgc ccgcacggcg ctgtggaatg
     1921 cgctccatgc ccgccgccat ggcggccagt tcatcctcag actcgacgat accgacgtcg
     1981 aacgctcgca ggaaatctat gccgaggcca tccagcagga tctcgcctgg ctcggcatcc
     2041 gctgggatcg gctggagcgt cagtcgctgc gactggatcg ctatggaatg gcgctggagc
     2101 ggctgaaggc cgccgggctc gtctatccgg cctatgagac acccgatgag ctggagcgca
     2161 agcgcaagcg ccagcaggcg cgcggcctgc cgccggtcta tgaccgggcc tcgctgaagc
     2221 tgaccgaggc cgaacgcacc gctctcgaag cccgtggccg gcatccgcac tggcggttca
     2281 gactgtccgg cgcgacggtg gaatggcagg atggcgtgcg cggtccctgc catatcgaca
     2341 ccggctcgct atccgatccg gtgctggtgc gcgaggatgg caccgtactc tacaccttcg
     2401 cctcggtggt tgacgatgcc gatatgggcg tcaccgatat catccgcggc gaggatcatg
     2461 tcgccaatac ggctgtgcag atcgagctgt tccacgcgct gggtgtcgca ccgccgcgtt
     2521 ttgcccatca caatctcatc gtggccgctt ccggcgaggc gatgtccaaa cgcaccggcg
     2581 cgctgtcgat ccggtcgttc cgcaaaggcg gcgcggatcc gatggcggtg gcggcggtcg
     2641 cggtcctgac cggcacatcc gaaccggtgc gtccgctcgc ctcgcttgat gacctggtgc
     2701 cgctcgccga gctttccggc atatcgcgtg ccctgaccaa attcgatccc gccgagatcg
     2761 gccatctgac gacgcggctg ctgcatacca agccctatgc cgctgtgcgg gatcggctcg
     2821 ccgccgcagg catcgacggc gcgcgcgccg aactgttctg ggatgcggtg cgcgccaacc
     2881 ttgccgacat ggaagaggct gtgagttggt ggcatgtcgt gaacgggccg atcgcgccgc
     2941 atgtccggtc cgatccggag ctgatcgaga ccgcccgcag gctgctgcca cccgaaccct
     3001 gggacgagac gacctggggc atctggtgcg ctgccgtgac cgccgcgacc ggtgcgcgcg
     3061 gcaaggcgct gttcctgccg ctcaggctgg cgctgaccgg gctcgaccac ggtccggaac
     3121 tgaaacgcct gctgccgctt atcgggcggg cggaagcgga acggcggctc gtctgatcga
     3181 ggcggttgtc tccgggtcta tcggctgcct gctggcgaca ggtcgcgccg gtgccggtct
     3241 cgtggcggcc tgatccggtt tgatgctggt cctgagcgcg ggcttggcat cggcgatggc
     3301 gggtttgccg gcctttttgc tcttggcggc ctttctgggc gctgccgcct cagccacgat
     3361 tttctcggct ggatcggccg gccgggcaag ttccagcgag cgctcgggcg tgaccagaat
     3421 atcgtccttg ccctgtttga tcatcggctc gacatgggcc agcacctcgc cccagctctg
     3481 gttctgggcc ttgcaggcgc aggaggcatc gcgctccttg cgatacttga aggcattcgg
     3541 caaagcggca taacgctggc catattgatt ggccgcctgc ttgagatcac cgccgcgcgg
     3601 catgaaatac acctcggtcg gcgcggaagg gcaggccagt tggcaggctt ccgcatagcg
     3661 gtcgctgcca tgggcgccct cgtaattgac cgggaagaag aacccgtcgc aggtgcgcac
     3721 gcagaccgcc tggctgcccc cgccgcggaa cggtggcagc ttcaccggct tcctgaccgg
     3781 gcggtcgaga tcggcgcccg gatcggatgg cttggggtcg atggtgccgg ctgcggccgt
     3841 gcgggtgtcg gcagccttgc cggcagaacg gggcaccggg ggcgcggcgg tgtcagcttt
     3901 tgcagcgacg gctttggccg ttccggcctt ggcaggcgcc ggggaggcgg attgctggct
     3961 ggtcctggcc gccttgccgg caggcggcgg cgccgcaaga ttcatcggtg cctcggtcgt
     4021 ggctggacga atgccgagca gattggcaag aaatcccggg ctgccactgc tgcccgtctg
     4081 cgcgccggcg gcctgggaca ctaccagagc cagcatcatg gcaggaatac gcaaacgcaa
     4141 cttggacatc gctcaccgcg cggaacgcaa ccttggccgc agatgagaat ttttgccgca
     4201 gaatgtttaa caattcggcg tggacgaggc cgacgacaac ctgcgccgtt ttagcctgtg
     4261 cgctggcgca caggttggtt cattggctga gcgaaagcgc cggggtgagc acgcgcactt
     4321 gcttcttttc gccgcccttg ctgttgactt cgcgcgtctc gccatcgccg gtgccgagca
     4381 tgcgattgcc ggagacggcc gggccgattc cagcggtggc cgtgctgttg gtggcgaaat
     4441 tggtgttgag ctgcatttcg accggttctt ccggtgcagt cttgcctttg acgccacgca
     4501 ggcgcacctg ttgcggccgc gccatctggt tggagcgctc ttcggtcacg gtgatatcgt
     4561 tcgcccggtc ccgcaccagc ttgtcggcat cgcgcagggc cgaggcccag ctctgctgcg
     4621 gctgcttgca ggaacacgac ggatcatagg ccttgcgata ctggaaggcg ttgggaaggg
     4681 cggtatagcg ctgcccgtcc tcggcgcggg c
//
LOCUS       DAOMXG010000181         4676 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_9819_length_4676_cov_9.976845, whole genome shotgun sequence.
ACCESSION   DAOMXG010000181 DAOMXG010000000
VERSION     DAOMXG010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4676
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9819_length_4676_cov_9.976845"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="PLQ11_10745"
     CDS             <1..576
                     /locus_tag="PLQ11_10745"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRK25420.1"
                     /translation="ELGAIEFRIVTDPAEKPAALAAFLALEQSGWKARTGTALACTPE
                     TRAFAEQAFLSDASEIDVLTLNGQPLAMTINLVRDEAMFAVKTAYDEAYSSFSPGVHL
                     DMWTVRKLFEPGTGYSRIDSCAIPGHPIEAVWTDKEPIISLAMATRPDVTQARIDQFT
                     RLMVTFNRLKTWVKEQIGSKGEAGDGEAKPA"
     gene            complement(630..1442)
                     /locus_tag="PLQ11_10750"
     CDS             complement(630..1442)
                     /locus_tag="PLQ11_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007566378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HRK25421.1"
                     /translation="MTKRRQIRRRASRGLRALLGLSLTALLASCSVQEQFNRYPAKGD
                     IKPHPGVARASSFPIQGIDISKWQGTIDWQAVRGAGTRFVFIKATEGGDHVDPKFLDN
                     WNGAKAAGVARGAYHFVYWCRPAHEQVVWFTQHIPQEPDALPPVLDVEWNSHSKTCPQ
                     RISKELALEKIRLMLDELEKHTGKRPVIYTDIPFHEEVLEGQFRDYVFWIRSTAAEPQ
                     TRYRDRDWGFWQFTTTGRVPGIAGNVDRNAFFGGEKEWAMFVSGKPMQISNK"
     gene            complement(1489..2901)
                     /gene="mgtE"
                     /locus_tag="PLQ11_10755"
     CDS             complement(1489..2901)
                     /gene="mgtE"
                     /locus_tag="PLQ11_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HRK25422.1"
                     /translation="MLGSDLPVTEPLAQPETFRDEEGEVRASFVASVATAIATENRDA
                     LAALVGDLHESDLGELIEALESEDRPRLIALLGRDFDFTALTEVDDAVREEILEEVPN
                     EAIAEGVRDLDTDDAVYILEDLDAADQEEVLAKLPADERGMLERSLDYPEESAGRRMQ
                     TEFIAVPPFWTVGQTIDFMRDAADLPGKFYQLFVIDAERRVLGGVGLDQLLRSKRPVR
                     IEAIMEPEEHQVRVDEDQEEVADIFKRYNLVTMPVIDADERLVGVLTFDDIVDVIEQE
                     ADEDIRALAGVKPDEELSDSVWTLARGRFSWLFINLITAFLASSVLKGFAGQLEKMVA
                     LAVLAPIVASQGGNAATQTMTVVVRAIATRELDRANALRVIGRELLAGSLNGLAFAVI
                     TGLIAAYWFGTYDLGIVIGIAMIVNLIAAALGGATIPLVLDAFGIDPAVSSAPFVTTV
                     TDIVGFFAFLGVATLWFGLA"
     gene            2979..3695
                     /locus_tag="PLQ11_10760"
     CDS             2979..3695
                     /locus_tag="PLQ11_10760"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRK25423.1"
                     /translation="MRRRDILLASLAAALPGPAGAAPGRRLVVPVAGGPVYGRKHYPA
                     SLPLADGEVVLTFDDGPAAGTTPRVLSALAAHGAKATFFLIGRHAAGLPHLAERIARD
                     GHSIGNHTFSHPWTIDRLSHERALADIDQGAAAIEKALGGAGRLSPFIRFPGFVETPA
                     LLADMQRRSLAVFGADLWASDWNVMAPQAQLDLVMGRLMRARKGIVLFHDTREQTAAM
                     LPAFLAALEAKGFRVVHAVA"
     gene            3771..4427
                     /locus_tag="PLQ11_10765"
     CDS             3771..4427
                     /locus_tag="PLQ11_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25424.1"
                     /translation="MAMKWNACAGLAISALAGAAMAQQPGMKVPPSTPSATDAIMDKS
                     AAQASQKQLRFAPLPVPDKLKSLMKQGVQSTSTDLGASGVVSPNTPKVKMGDVMAMLD
                     GTNVNWTVGIAWEYEVRFHGKSSGESNAQLRLWSLQGRRALIDCGFEGYLETKVKAGA
                     TAEYSYDGKVQKVPVENFRASFVLPLKSTGTVVIRPSYGANGVPDLVLKACEVTLLKN
                     "
     gene            complement(4463..4547)
                     /locus_tag="PLQ11_10770"
     tRNA            complement(4463..4547)
                     /locus_tag="PLQ11_10770"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4511..4513),aa:Leu,seq:gag)
BASE COUNT          894 a         1514 c         1436 g          832 t
ORIGIN      
        1 gaactgggcg cgatcgagtt caggatcgtt accgatccgg cggaaaagcc ggcggcgctt
       61 gcagcctttc tggcgctcga acaatccggc tggaaagcca ggaccggcac ggcgctggcc
      121 tgcacgccgg aaacccgcgc ctttgccgaa caggccttcc tgtccgacgc gtccgagatc
      181 gacgtgctca ccctcaacgg ccagccgctg gccatgacga tcaatctggt gcgcgacgag
      241 gcgatgttcg ccgtcaagac ggcctatgac gaagcctatt ccagcttcag tccgggcgtt
      301 cacctcgaca tgtggacggt caggaagctg tttgaacccg gcaccggcta cagccggatc
      361 gattcctgcg ccattcccgg ccatccgatt gaggccgtct ggaccgacaa ggaaccgatc
      421 atatcgctgg cgatggccac cagacctgac gtcacacagg cgcgcattga ccagtttacc
      481 cgcctgatgg tgacgttcaa ccggctgaaa acctgggtca aggagcagat cggcagcaaa
      541 ggcgaagctg gcgacggcga agccaagccg gcctgacccg gcggttgccg ccgcccaagc
      601 taccgatccg ccgatccgcc gcagaatgcc tatttgttgg atatctgcat cggtttgccg
      661 gagacgaaca tcgcccattc cttctccccg ccgaagaagg cattgcggtc gacgttgccg
      721 gcaattccag gcacccggcc ggtggtggtg aactgccaga agccccagtc acggtcgcgg
      781 tagcgggtct ggggttcggc cgccgtcgag cggatccaga acacgtaatc gcggaactgg
      841 ccttccagca cttcctcatg gaatggaatg tcggtgtaga tcaccggccg cttgccggtg
      901 tgcttttcca gctcgtcgag catcagccgg atcttttcca gcgccagctc cttggaaatg
      961 cgctgcgggc aggtcttgga atggctgttc cattcgacat ccagcaccgg cggcaaagca
     1021 tcgggctcct gcggaatatg ctgggtgaac cagaccacct gttcatgcgc cggccggcac
     1081 cagtagacga aatggtaggc gccgcgcgcc acaccggctg ccttggcacc gttccagttg
     1141 tcgaggaatt tggggtcgac atggtcgccg ccctcggtgg ccttgatgaa gacgaagcgg
     1201 gtcccggccc cgcgcaccgc ctgccagtcg atggtcccct gccatttgga gatgtcgatc
     1261 ccctggatcg gaaagctcga ggcgcgcgca accccgggat gcggcttgat atcgcccttg
     1321 gcgggatagc ggttgaattg ttcctgcacc gagcaggagg ccagcaatgc cgtcaggctg
     1381 agccccagca gggcgcggag gccacgcgag gcgcggcgcc ggatctgtct gcgcttggtc
     1441 atccggtgac aattgaatcg aaactcttaa gcaatccgaa acgcagcgtc aggcgaggcc
     1501 gaaccacagc gtcgccaccc caagaaaggc gaagaatccg acgatatcgg tcaccgtcgt
     1561 cacgaacggc gccgaggaca ccgccggatc aatcccgaaa gcatcgagca ccagcggaat
     1621 ggtcgccccg ccgagcgcgg cggcgatcag attgacgatc atcgcaatgc cgatgacgat
     1681 gccgagatcg taggtcccga accagtaggc agcaatcagg ccggtgatga ccgcaaaggc
     1741 aagcccgttc aggctgccgg ccagcagttc gcgcccgatc acccgcagcg cattggccct
     1801 gtccagttcg cgcgtggcaa tggcgcgcac caccacggtc atcgtctggg tagcggcatt
     1861 gccgccctgg ctggcaacga tgggcgccag caccgccagc gccaccatct tctcaagctg
     1921 cccggcgaac cccttgagca cggaggaagc gagaaaggct gtgatgaggt tgatgaacag
     1981 ccaggaaaag cggccgcgtg ccagggtcca caccgaatcc gacagttcct cgtccggttt
     2041 cacaccggcg agggcgcgaa tgtcctcgtc cgcttcctgc tcgatgacat cgacgatatc
     2101 gtcgaaggtc agaacgccga ccagccgctc gtcggcatcg atcaccggca tggtgacgag
     2161 gttgtagcgc ttgaagatgt cggcgacctc ttcctggtcc tcatcgaccc ggacctgatg
     2221 ctcttcaggc tccatgatcg cttcgatccg gaccgggcgc ttgctgcgca gcaactggtc
     2281 gagcccgacc ccgcccagca cccgccgctc ggcatcgatg acgaacagct ggtagaattt
     2341 ccccggcaga tcggcagcgt cgcgcatgaa atcgatggtc tggccgacgg tccagaacgg
     2401 tggcaccgcg atgaattcgg tctgcatgcg ccggccggcc gattcttcgg gatagtcgag
     2461 gctgcgctca agcatgccgc gctcgtcggc gggcagcttg gccagaacct cttcctgatc
     2521 ggccgcatcg aggtcttcaa gaatgtagac cgcgtcgtcg gtatcaaggt cgcgcacacc
     2581 ttcggcgatt gcctcattgg gcacctcctc gaggatttcc tcgcgcaccg catcatcgac
     2641 ctcggtcagc gccgtgaagt cgaagtcgcg gcccagcagg gcgatcagcc gcggccgatc
     2701 ctcgctttcg agcgcctcga tcaattcacc gagatcggat tcgtgcaggt cgccgaccag
     2761 cgccgccagg gcatcgcggt tttccgtcgc gatggcggtg gcgaccgaag ccacgaacga
     2821 ggcgcgcacc tcgccctcct catcccggaa ggtttccggc tgagccagcg gttcggttac
     2881 gggcaggtca gagccaagca tcgcaaaccc attgccaaca ggggcaaaat cgcttctagg
     2941 atttgcctct cgccagcgca atgcaacggg tatgaaaaat gcgacgccgc gacatcctgc
     3001 tcgcttctct tgcggcggcc ttgcctggtc ctgccggcgc cgcaccgggt cggcggctgg
     3061 tcgtgccggt tgcgggcggt ccggtctatg gccgcaagca ctatcctgcc agcctgccgc
     3121 tcgccgacgg cgaggtggtg ctgaccttcg acgatggccc ggccgccggc accacgccgc
     3181 gtgtcctgtc tgccctggcc gcccacggcg ccaaggcaac cttcttcctg atcggccgcc
     3241 atgccgccgg cctgccgcat ctggccgaac gcatcgcccg cgacggccat tccatcggca
     3301 accacacctt ctcgcacccc tggaccatcg accggctcag ccatgagcgg gcgctggccg
     3361 acatcgatca gggcgccgcc gccatcgaaa aagccctcgg cggcgccggg cggctcagcc
     3421 cgttcatccg ctttcccggc tttgtcgaaa ctcccgccct gctcgccgac atgcagcgcc
     3481 gcagtcttgc cgtgttcggc gccgatctct gggccagcga ctggaacgtc atggcaccgc
     3541 aggcgcagct cgatctggtg atgggtcgtc tgatgcgcgc aaggaaggga atcgtcctgt
     3601 tccacgacac ccgcgagcag accgctgcca tgctgcccgc cttccttgcc gcacttgagg
     3661 caaagggctt tcgcgtcgtc cacgcggtgg cgtgacgtca cgcaaatgtg cgacgacatt
     3721 agcacgaaac gtcattaggc tgtgctcctc atcatcgatg gaggacggtc atggcgatga
     3781 aatggaacgc gtgtgcgggt cttgcaatca gtgctctggc gggagcggcc atggcacagc
     3841 aaccgggcat gaaagtgccg ccatcaaccc cttcggcaac ggatgcgatc atggataagt
     3901 ctgcggctca ggccagccaa aagcagctgc gatttgcccc cctgcccgtc cccgacaaat
     3961 tgaagtcatt gatgaaacag ggcgtccaga gcacctctac cgatctcggc gcgtcaggcg
     4021 tggtcagccc gaatactccg aaagtgaaga tgggcgatgt catggcgatg ctggatggca
     4081 caaatgtcaa ttggacagtc ggaattgcct gggagtatga agtgcgtttc catggcaagt
     4141 cctcgggtga gagtaacgcg cagttgcgct tgtggtctct tcaaggtcga cgcgcgctga
     4201 tcgattgcgg gtttgaaggc tacctggaaa caaaagtgaa ggcaggtgcc acggccgagt
     4261 attcatatga tggaaaagtg caaaaggtgc cggttgaaaa ctttcgtgct tccttcgtat
     4321 tgcccctgaa atcgaccggt accgttgtta tcagacccag ctatggagca aatggcgtac
     4381 cggaccttgt gctcaaggcc tgtgaagtta ccttgctgaa gaactgactg gacagcaaaa
     4441 agcccccgtt tccgggggct tttggtgcgg ccaagaggac tcgaacctcc acgggtctcc
     4501 ccactagcac ctcaagctag cgcgtctacc aattccgcca tggccgcctg ggacgtcgga
     4561 accggcgaac cggccccgtt ccgagggccg gtgactaaca aacgccgtgc cctgtgacaa
     4621 gccggaaagc aggcagcgcc ggattttctt gcccgcagca gcctggcggg cggcgg
//
LOCUS       DAOMXG010000182         4624 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_9960_length_4624_cov_9.713504, whole genome shotgun sequence.
ACCESSION   DAOMXG010000182 DAOMXG010000000
VERSION     DAOMXG010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4624
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9960_length_4624_cov_9.713504"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..1442
                     /locus_tag="PLQ11_10775"
     CDS             3..1442
                     /locus_tag="PLQ11_10775"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HRK25425.1"
                     /translation="MPGSVAVPKGRWSGPGIVHLGIGAFHRAHQAVMLQVAMDAGETG
                     WGIIAASLRSPDTRDALRPQDGLYTVAVKSPEGERLQVIGAVGDVLVAREDPGALIAC
                     MSDPRIRIVSMTVSEKGYCHNPATGALNEAHPDIIHDLANPDRPRSAPGFLVAALARR
                     RAAGVAPFTVLCCDNLPSNGRTVRRVVVRYAELVDPALGRFVAGEVAFPSTMVDRIVP
                     ATTDADRDRIAAALGVRDAWPVVTEPFTQWVIEENFPTGRPRWELGGAQFVRDVEPFE
                     LMKLRMLNGAHSTLAYLGAVAGHETVADASNDPAFAAMLRGLWQEIIPSLAEPEGQQP
                     SAYAEALLARFQNTAIRHRLLQIAMDGSQKLPQRLLGTVADNLATGRPIRFLALGVAA
                     WMRFVMGASEAGGRHEINDPLAGRITAVTTAAGRDARALAEGLLAIEAIFAPALVANS
                     EFRAAVTGQLASLIENGVVPTIAAQSKLL"
     gene            complement(1511..2179)
                     /locus_tag="PLQ11_10780"
     CDS             complement(1511..2179)
                     /locus_tag="PLQ11_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HRK25426.1"
                     /translation="MASLMTADALTALAQVIMIDLVLAGDNAIVIGLAVAGLPAEMRA
                     KAIMMGIAAATVLRIIFAALTTKLLGIIGLLFAGGVLLAWVCWKMWRELRTSHQEEVE
                     AEEALEGVDLNADGEIAGHAPRKTFAQAAWQILIADVSMSLDNVLAVAGAARDHPSIL
                     VIGLALSIALMGLAANFIAKLLNKHRWIAYVGLAIIVYVSLEMIYKGALEIWPYLNGA
                     ARPA"
     gene            complement(2326..2784)
                     /gene="aroQ"
                     /locus_tag="PLQ11_10785"
     CDS             complement(2326..2784)
                     /gene="aroQ"
                     /locus_tag="PLQ11_10785"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HRK25427.1"
                     /translation="MNRLIFVLNGPNLNLLGKRQPEIYGRETMADVERLCRAEAERHG
                     LSLRFHQSNREYELIDWVHEARGIAGGLLINPGAFTHTSIALLDALNAFEGPVIECHI
                     SQVHRRETFRHHSYVSLRADAVLAGFGTQGYGLAIQRLARLIDAAGQTGA"
     gene            complement(2821..4122)
                     /locus_tag="PLQ11_10790"
     CDS             complement(2821..4122)
                     /locus_tag="PLQ11_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRK25428.1"
                     /translation="MSPAFFWCIILLFALAGIGAPIALSMIASSILYLLLKGQDIGLA
                     AEQMIQGLYDGFLLLAIPLFIVAANIMNAGTISERLLQFCLALVGRFRGGLGHVNVVA
                     NMIFAGMSGSAVADAVGLGKIIIGMMRKDNRYSPGYAAAITAAASTIGPIIPPSIPMV
                     MYALVSDTSIGYLFLGGFIPGVIMGIALMLLNARMARKENVPLEEPVPRSELPRLTVR
                     AFPALLMPIILLFGIYGGATTPTEAAAIAAFYALLVATFLYRAVSFKVLHGVLAESGR
                     ATASVGLVIGASLIFNYIVAAENIPAKMSVLLQGLSVTPLTFLLGVNVLFLILGCLLD
                     ATTIILVIIPLFIPTCKALGIDMVHFGVTAVVNCMIGLITPPYGVLLFVINAITGIPL
                     KTIIGAIWPFITVLVTALLIMLLVPETVLFLPKMFGYVVGR"
     gene            complement(4119..>4624)
                     /locus_tag="PLQ11_10795"
     CDS             complement(4119..>4624)
                     /locus_tag="PLQ11_10795"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HRK25429.1"
                     /translation="GAMRALLHFLYRRAEDVMVLLVAVMFAAFLVQIGSRYLFNAPTD
                     WTHELILITWLWLIFWGAAFLLKDEDHVKFDVLYNLGGERIRRAMALVTALALSVAFL
                     ASVPATWDFITFKKLRSSDSLGIRLDVIFSVYMVFLAGTIIHYGLRSWRLMRGDSLAT
                     LEKEEAL"
BASE COUNT          876 a         1477 c         1487 g          784 t
ORIGIN      
        1 ccttgcccgg ctcggttgcg gttcccaaag gccgctggtc aggtcccggc atcgtccatc
       61 tgggtatcgg cgccttccac cgcgcccatc aggcggtgat gctccaggtg gcgatggatg
      121 ccggcgagac cggctggggc atcatcgccg ccagcctgcg cagccccgac acgcgcgacg
      181 ccttgaggcc gcaggacggg ctttataccg tcgcggtcaa gtcgccggag ggcgagcggc
      241 ttcaggtgat cggtgctgtg ggcgatgtgc tggttgctcg cgaagacccc ggcgcgctga
      301 ttgcctgcat gtccgatccg cgcatccgca tcgtttccat gacggtgagc gagaagggct
      361 attgccacaa tccggccact ggcgcgctga acgaggcgca tccggatatc atccatgatc
      421 tcgccaatcc ggatcggccg cgctcggcac cggggtttct ggtggcggca ctggcccgcc
      481 gccgcgcggc cggtgtggcg cccttcacgg tgctgtgctg cgataatctg ccatcgaacg
      541 gccggacggt gaggcgtgtg gtcgtgcgtt atgccgaatt ggtcgatccg gcgctgggcc
      601 gttttgtcgc cggcgaggtc gcctttccct cgaccatggt tgaccgcatc gttccggcga
      661 cgaccgacgc cgatcgtgac cgcatcgctg cggcgcttgg tgtgcgcgac gcatggccgg
      721 tcgtcaccga accattcacc caatgggtga tcgaggagaa tttcccgacg ggccggccac
      781 gctgggaact gggcggggcg caattcgtcc gcgatgtcga accgttcgaa ctgatgaagc
      841 tgcgcatgct gaacggcgcg cattcgacgc tggcctatct cggtgctgtc gccggccatg
      901 aaaccgtggc cgatgcgagc aatgatccgg cctttgccgc aatgctccgg gggctgtggc
      961 aggagatcat tccgagcctg gccgagcccg agggccagca gccttcggcc tatgccgagg
     1021 cgctgctcgc ccgctttcag aataccgcga tccgccaccg gctgctgcag atcgccatgg
     1081 acggttcgca gaaactgccc cagcgcctgc tgggcacggt tgccgacaac ctcgccaccg
     1141 gccggccgat ccggtttctg gcattgggcg tggccgcctg gatgcggttt gtcatgggcg
     1201 ccagtgaggc gggcggccgg cacgagatca atgatcctct ggccggccgg ataaccgccg
     1261 ttacgacagc ggccggcagg gatgcgcggg cgctggcgga gggtttgctc gccatcgagg
     1321 cgatcttcgc acccgctctc gtcgcaaaca gcgagttccg cgccgccgtg accggacagc
     1381 tcgccagcct gatcgaaaac ggcgttgtgc cgacgatcgc ggcgcagtct aagctattgt
     1441 aatcgatcat gttatctctc gtcttgatgg ggtcatcaaa acgagagcat gatcttaccc
     1501 cgtcccggcc tcaggccggc cgggcggcgc cgttcagata gggccagatc tccagcgccc
     1561 ccttgtagat catctcaagg gacacataga cgatgatggc gaggccgaca taggcgatcc
     1621 agcggtgctt gttcagcagc ttggcgatga aattggcggc aaggcccatc agcgcgatcg
     1681 acagggcaag accgatgacc agaatggatg gatgatcgcg ggcggcgccg gccaccgcca
     1741 gcacgttgtc gagcgacatg gagacgtcgg cgatgaggat ctgccaggcg gcctgggcga
     1801 acgtcttgcg cggcgcatgc ccggcgatct cgccgtcggc gttaagatcg acgccttcaa
     1861 gggcttcctc ggcctcgacc tcctcctggt ggctggtgcg caattcgcgc cacatcttcc
     1921 agcagaccca ggccagcagc acgccaccgg cgaacagcag gccgatgatg cccagcaatt
     1981 tggtcgtcag cgcggcaaag atgatacgca ggaccgtcgc tgcggcgatg cccatcatga
     2041 tggccttcgc ccgcatctcg gccggcaggc cggccaccgc aaggccgatc acgatggcat
     2101 tgtcgcctgc cagcacgagg tcgatcatga tgacctgagc cagggcggtc agcgcatcgg
     2161 cggtcatgag actcgcgata aattccattt catccagcct gaaagttctg tgttccgccc
     2221 gccgcccggc ctgcttccgg ggccggttca tcgggcaagg tcatggcatt ttgccgccgc
     2281 aatgacaaga ctgccgtcag gctttgcggg ccgcgccggc accgcttatg cccccgtctg
     2341 gcctgccgca tcgatcagcc tggccagccg ctgaatggcg agcccgtacc cttgcgtgcc
     2401 gaagcctgca agcaccgcat cggcgcgcag cgagacatag gaatggtggc ggaaggtctc
     2461 gcgcctgtgc acctggctga tgtggcattc gatgaccggc ccctcgaagg cattgagcgc
     2521 atcgagcagg gcgatcgagg tatgggtgaa ggcaccggga ttgatgagaa ggccgcctgc
     2581 gatcccgcgg gcctcgtgca cccagtcgat cagttcgtat tcccggttcg actggtgaaa
     2641 gcgcagtgaa agcccatgcc gctccgcttc ggcgcgacac agccgctcca catccgccat
     2701 ggtttcacgg ccatagatct ccggctggcg tttgccgagc aggttgaggt tgggcccgtt
     2761 gagcacaaaa atcaggcggt tcatgacttg ctccctgggg gcgaccggat cgccgaaagg
     2821 tcacctgccg accacatagc cgaacatctt cggcaggaac agcaccgttt ccggcaccag
     2881 cagcatgatc agcagcgctg tcaccagcac ggtgatgaac ggccagatcg cgccgatgat
     2941 tgtcttgagc ggaatgccgg tgattgcgtt gatgacgaac agcagcacgc cgtagggcgg
     3001 ggtgatcaac ccgatcatgc aattgaccac ggcggtgacg ccgaaatgca ccatatcgat
     3061 gccgagcgcc ttgcaggtgg ggatgaacag cggaatgatc accaggatga tcgtagtcgc
     3121 atccagcagg cagccgagta tcaggaacag gacgttgacg ccgagcagga aggtgagcgg
     3181 ggtcacgctc agcccctgca gcagcaccga catcttcgcc gggatattct ccgccgcgac
     3241 gatgtagttg aagatcagcg aggcgccgat caccagcccc accgaggccg tcgcccgccc
     3301 gctttcggcc agcacgccgt gcagcacctt gaacgaaacc gcccggtaga ggaaggtcgc
     3361 caccagcagg gcatagaagg cggcgatggc cgccgcttcg gtcggggtcg tggcgccgcc
     3421 atagatgccg aacagcagga tgatcggcat caacagggcc ggaaaggccc gcaccgtcag
     3481 ccgcggcagt tccgatcgcg gcaccggttc ttccagcggc acgttctcct tgcgcgccat
     3541 gcgggcgttg agcagcatca gcgcgatgcc cattatcaca ccgggaatga agccgccgag
     3601 gaacaggtat ccgatcgagg tgtccgagac gagcgcatac atcaccatcg ggatcgaggg
     3661 tggaatgatc gggccgatgg tcgaggctgc cgctgtgatc gccgctgcat agccgggcga
     3721 atagcggttg tccttgcgca tcatgccgat gatgatcttg cccaggccca ccgcatcggc
     3781 cacggccgag cccgacattc cggcaaagat catgttggca acgacattga catggccgag
     3841 cccgccgcgg aaccgcccca ccagcgccag gcagaattgc agcaaccgct ccgagatcgt
     3901 ccccgcgttc atgatattgg cggcgacgat gaacaacggg atcgccagca gcaggaaccc
     3961 gtcatagagg ccctggatca tctgttcggc ggcaagcccg atgtcctgcc ccttgagcaa
     4021 cagatagagg atcgacgagg cgatcatcga cagcgcgatc ggcgcgccga tgcccgccag
     4081 cgcgaacagc aggatgatgc accagaagaa ggccgggctc acagtgcctc ttccttctcc
     4141 agtgttgcga gcgaatctcc gcgcatcagc cgccaggagc gcaggccata gtgaatgatc
     4201 gtgccggcca gaaacaccat gtagaccgag aaaatcacat caagccggat gcccagactg
     4261 tcggatgagc gcagcttctt gaaggtgatg aaatcccacg ttgccggcac cgaggccagg
     4321 aaggcaacgc tgagcgccag agctgtgacc agcgccatgg cccgccgtat cctttccccg
     4381 ccgagattgt agagcacgtc gaatttcacg tgatcctcat ccttcagcag gaaggcggcg
     4441 ccccagaaga tcagccagag ccaggtgatc aggatcagtt catgggtcca gtcggtcggc
     4501 gcgttgaaca ggtagcgtga gccgatctgc accaggaagg ccgcaaacat caccgcgacg
     4561 agcaggacca tcacgtcctc ggcccgcctg tagagaaaat gcagcagcgc gcgcatcgct
     4621 cccc
//
LOCUS       DAOMXG010000183         4597 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_10046_length_4597_cov_11.386614, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000183 DAOMXG010000000
VERSION     DAOMXG010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4597
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10046_length_4597_cov_11.386614"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..618
                     /locus_tag="PLQ11_10800"
     CDS             121..618
                     /locus_tag="PLQ11_10800"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HRK25430.1"
                     /translation="MPLDDLIRDMRAALALAPIIACEAAAIHAQTVKEAPGAMDQRVL
                     ARIRGGFDVPARSYIAAIQQREVLRPQFAARLAAVDCLALPTVPIVAPKIAPLEADTD
                     LFAATNMMALRNTSVFNFFNTPAISLPLPVDGLPVGLMLAALPMQDRRLLAMAAGVEA
                     ALKAG"
     gene            complement(683..1930)
                     /locus_tag="PLQ11_10805"
     CDS             complement(683..1930)
                     /locus_tag="PLQ11_10805"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRK25431.1"
                     /translation="MQPVSLQPRFPADHRFGVLDAWRGVCALLVTIVHIPVLHALQGT
                     QVFMNMQMFVDFFFVLSGFVMSHAYGRSLDGPVKLEGFLIRRFGRIYPLHLAILLGFV
                     ALELVKLGVLTLAKMQVDGDPFTGPRSYATLASNLFLMQSFNLHGMTTWNGPAWSISV
                     EFYAYMVFAAAVLFAGMRTALFTALATVALAGLVAFSPNYIFATHDYGFLRCLYGFFV
                     GCVVYQAVMRRGSGRLAGTAAELGSVALLIGFMLLTGQNATSYLAPLVFAVVVYVFAF
                     ERGIVSKLLATGPAQALGLWSYSIYMVHMLIFTVEKMVLGLVAKKAILGFAIVQVPTG
                     RLWTFDNLALDTALFTLQIVLTLAISKLTYDWIEDPWRRRFATIARRREQALRAPAEA
                     PSSSDCPAGIVTSLRVARGPGLR"
     gene            complement(1932..3125)
                     /locus_tag="PLQ11_10810"
     CDS             complement(1932..3125)
                     /locus_tag="PLQ11_10810"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRK25432.1"
                     /translation="MTYVATFDHQLDLPRVDAKARHQGHEVILHHGPQAVRALLAGFQ
                     PRAECSVFQHSAWISAWIDTLGTTRRCDSLAAEVRQRSDGMVVMVLPLIRRWSRGLRL
                     IEQPDFGIADYGGALIAPGFAPDAAAMRAIWADLVAALPKADLLRIYKLPERTGTLAN
                     PLLLLKDVRRSHLSAFTSPLHGLPFDILRIGMTRKRLRDLNRRLGNLKAMGDLEFSTA
                     SNPQEAEKLFDIICAQRAARFAELGRPNCLAHADMRAFFRALLYPTDGKPLAVVQATR
                     LNGTVIAASYALNSGNSLCMIFPTVAKGEWMSHSPGLQHFRFSMEWAAEQGLTAYDFT
                     IGSEQYKAELGAVEHALYELYQPLTLRGRLASATLTTRWHIRERPALASRLRKVKQFL
                     SFGRT"
     gene            3492..4232
                     /locus_tag="PLQ11_10815"
     CDS             3492..4232
                     /locus_tag="PLQ11_10815"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HRK25433.1"
                     /translation="MNSLARLMRILDQFEAAPGILTADQLHERLGYSRSTLYRYLKAL
                     SDNGFIVSLHGNGFSLGPRIIELCSALTARDPLILSSTPVMQDLAREFKGMVSLHRRF
                     RERVVCVHRTADAVFAKGFDHAGRVLSLTDGAAGRVVLAHLCSASVRKLYQARPHSFD
                     AAGLGSGLPAVRQTLRQIRDRGYDVDWCHDADGPVMVAAPILDQTAVGIGSLCLTMPS
                     HEISGDRILGVAEQVIDCTEVIGYALNG"
     gene            complement(4222..>4597)
                     /locus_tag="PLQ11_10820"
     CDS             complement(4222..>4597)
                     /locus_tag="PLQ11_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRK25434.1"
                     /translation="PALSVLPLERRGWVSGAESLVTDLLATAGLINLGASITPEGSRF
                     SLEWLVTLKPDRLLLAGRNPRADDQGLALLRHPALAAWMRPERTLILPDALTVCGGPM
                     IADALDQLADEIARTDAAALSR"
BASE COUNT          887 a         1371 c         1520 g          819 t
ORIGIN      
        1 tgagccgatc caacccgaac tggatgagga aggcttccgt cgtgccgtag gtgacttcct
       61 gcgacggctt tcgaggccgg gatcgaccgg atcgcccgtg ccggcgcccg cgtcgtccgc
      121 ttgccgctcg atgacctcat ccgcgacatg cgcgcggcac tggcgctggc tccgatcatt
      181 gcctgtgagg cggcagcgat ccatgcccag accgtgaaag aggccccggg cgccatggat
      241 cagcgggttc tcgcccgcat tcgcggaggc ttcgacgtgc cggcacgctc ttacatcgct
      301 gccatccagc aacgcgaggt gctgcggccg cagtttgcgg cgcggcttgc ggctgtcgat
      361 tgcctggccc tgccgacagt gccgatcgtg gcgccgaaga tcgcgccact cgaggcggat
      421 accgacctgt tcgccgccac caacatgatg gccctgcgca atacatcggt gttcaatttc
      481 ttcaacacgc cggcgatttc gctgcccctg ccggtcgatg gtctgccggt cggccttatg
      541 ctggcggcac tgccgatgca ggatcggcgg ctgctggcga tggcagccgg cgtggaagcg
      601 gccttgaagg cagggtgatg ccggaagacc gaaccgccgg ccctgcggcc ggcggctggt
      661 gtgttcggaa cgggcggacc gctcagcgca ggccggggcc gcgggcaacc ctcaggctgg
      721 tgacgatccc ggccggacag tcggaggaag agggcgcctc cgccggagcc ctgagtgcct
      781 gttcgcggcg gcgggcaatg gtggcaaagc gcctgcgcca gggatcctcg atccagtcat
      841 aagtcagttt ggagatagcc agggtcagca cgatctgaag ggtgaaaagg gctgtatcga
      901 gcgccaggtt gtcgaacgtc cacagccgtc cggtgggtac ctgcacgatg gcaaagccga
      961 ggatcgcctt tttggcaacc agccccagca ccattttctc gacggtgaag atcagcatgt
     1021 gaaccatgta gatcgagtag gaccacaggc cgagcgcctg ggcagggccc gtggccagca
     1081 gcttcgagac aatgccgcgc tcgaaggcaa agacatagac caccactgca aagaccaatg
     1141 gcgcaagata gctggtggcg ttctgaccgg tgagcaacat gaagccgatc agcaacgcca
     1201 ccgagcccag ttcggcggcc gttccggcaa ggcggccgga gccacgacgc atcaccgcct
     1261 ggtagacgac gcagccgacg aagaagccat agaggcagcg taggaagccg tagtcatggg
     1321 tggcgaagat atagttcggc gagaaggcga caagccccgc cagcgccacg gtcgccagag
     1381 cggtgaacag cgccgtgcgc atgccggcaa acaggacggc ggcggcaaaa accatgtagg
     1441 cgtagaattc gacgctgatg ctccaggcag ggccattcca ggtcgtcatg ccgtgcaggt
     1501 tgaacgactg catgagaaac aagttggagg caagcgtcgc gtaggagcgc gggccggtga
     1561 agggatcgcc gtcaacctgc atcttggcca gggtgagaac gccgagcttg accagttcca
     1621 gcgcgacaaa gccgagcagg atcgccagat gcagcggata gatgcggccg aaacggcgga
     1681 tgaggaagcc ctcaagcttg accggcccgt ccagagaccg gccataggca tgcgacatca
     1741 cgaagccgga caggacgaag aagaaatcca cgaacatctg catgttcatg aacacttgcg
     1801 ttccctgtag cgcatggaga accggaatgt gaacgatcgt taccagcagg gcacagacac
     1861 cgcgccatgc atcgagcacg ccgaagcggt ggtcggcggg gaagcgaggt tggagtgata
     1921 caggctgcat gtcaggttct cccgaacgac aggaattgct tcactttacg caaacggctt
     1981 gccagcgccg ggcgctcgcg gatgtgccat cgggtggtga gtgttgcgga tgccaaccga
     2041 cccctaagag tcagcggctg gtagagttcg tagagtgcgt gttcaaccgc tcccaactcc
     2101 gccttgtatt gttccgagcc gatggtgaaa tcgtaggccg tcagtccctg ttcggcagcc
     2161 cattccatgc tgaaacggaa gtgctgcagg cccggtgaat gcgacatcca ctcgcctttc
     2221 gccacggtcg ggaagatcat gcagaggctg ttgccggagt tgagcgcata ggaagcggca
     2281 atcactgtgc cattcagccg cgtggcttgc acgacagcca gcggctttcc gtccgtcggg
     2341 tagagcaggg cgcgaaagaa agcacgcata tcggcatgcg ccaggcagtt gggccggccg
     2401 agttcggcaa agcgcgcggc ccgttgcgcg cagatgatgt cgaacagctt ttcggcctct
     2461 tgcggattgc tggcggtgga aaactcaagg tcgcccatcg ccttgaggtt gccgagccgg
     2521 cgattgagat cgcgcaggcg ttttcttgtc atgccgatcc tgaggatgtc gaacggcagg
     2581 ccgtgcagcg gcgaggtgaa ggccgaaagg tgactgcgcc ggacgtcctt cagcagcagc
     2641 aatggattgg cgagcgttcc ggtgcgttcc ggcagcttgt agatacgcaa gagatcggct
     2701 ttcggcaggg cggcaaccag atcggcccag attgcacgca ttgcggcggc atcgggggca
     2761 aagccgggag caatcagcgc gccgccataa tcggcgatgc caaaatcagg ctgctcgatc
     2821 aggcgcaagc ctcgggacca gcgacgaatg agcggcagaa ccatgaccac catgccgtcg
     2881 ctgcgctgac gcacttcagc ggcaagacta tcacagcggc gtgtcgtgcc gagcgtatcg
     2941 atccatgcag agatccaggc ggagtgctgg aacacgctgc attcggcccg cggctgaaag
     3001 ccggccagaa gcgcacgcac ggcctgaggg ccatgatgca ggatgacctc atggccctga
     3061 tggcgcgcct tggcatcaac tcgtggaaga tcaagctggt gatcgaatgt agcaacgtag
     3121 gtcatcgaac cctgtttccg tcagcggttg ttccgtcgta ttccggcaca ggaagtgtgc
     3181 cttttcggat cagccgtcgt ttgtcgtggc ttgtggcgca gttccagcaa ggctcatgcc
     3241 agcgcgggga acctgaccgc agacgggtgc gggcccgtca caggccgtag atgagcttcg
     3301 gccgccgatt gcagcttttt gtcacattgt gagttttgcc acatacggtg cggcgccgtg
     3361 ggccagtgtc ggcatgcatg tcatttgttc gaatgacgcc gcgtctgggt gatctcatgc
     3421 tgacccaagg taatctcatg gtggcctgac gcgatcctga ttggccttat gcaataaatg
     3481 ggactctgtc cgtgaatagc ttggcgcgcc tgatgcggat tctcgaccag ttcgaagctg
     3541 cacccggaat attgaccgcc gatcaattgc atgaacggct gggctattca cgatccacgc
     3601 tttatcgcta tctgaaggct ctgtcggaca acggtttcat cgtatcgttg catggcaatg
     3661 gcttttcgct cggcccgcgg atcatcgagc tctgttcggc gctgaccgcg cgcgatccgc
     3721 tgatcctgtc cagcacgccg gtgatgcagg acctggcgcg tgagttcaaa ggaatggtgt
     3781 cgctccaccg ccggttccgt gagcgtgtgg tctgcgttca ccgaacggcc gatgcggttt
     3841 tcgccaaagg cttcgaccac gcgggccgcg tgctgtcgct gacggatggc gctgccgggc
     3901 gcgtggttct ggcccatctc tgctctgcat cggtgcgcaa actctatcag gccaggcctc
     3961 acagcttcga cgccgccggt cttggcagcg gattgccggc cgtccgccag acgctgcgcc
     4021 agatccgcga tcgcggctat gatgtcgact ggtgtcatga tgcggatggt ccggtgatgg
     4081 tcgcagcgcc catcctcgat cagaccgctg tcggaatcgg cagtctttgc ctgacgatgc
     4141 cgtcacacga gatatcgggc gatcgtatac tcggcgttgc cgaacaggtc atcgactgca
     4201 ccgaggtgat cggctacgcc ctcaacggct gagcgcggca gcgtcggtcc gcgcaatttc
     4261 atcggcgagc tgatcgaggg cgtcggcgat catcggccca ccacagacag tcagcgcatc
     4321 gggcaggatc agcgtgcgtt cggggcgcat ccacgccgcc aatgccggat gccgcagaag
     4381 cgccagtccc tgatcatcgg cgcgggggtt gcgcccggcg agcagcagcc ggtcgggttt
     4441 gagggtcacc agccattcga gcgaaaagcg gcttccctca ggcgtgatgg acgcgccgag
     4501 gttgatcagt ccggcggtag ccagaagatc ggttaccagg ctctcggcgc cgcttaccca
     4561 gccgcgacgc tccagcggca gaacggacag ggccggg
//
LOCUS       DAOMXG010000184         4577 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_10100_length_4577_cov_9.244582, whole genome shotgun sequence.
ACCESSION   DAOMXG010000184 DAOMXG010000000
VERSION     DAOMXG010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10100_length_4577_cov_9.244582"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="PLQ11_10825"
     CDS             complement(<1..269)
                     /locus_tag="PLQ11_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25435.1"
                     /translation="MSGTVRDAMKKFAGLVVACLATILAAASFVPGFWSWMPGGIASS
                     ARQLVATLPQSAQPMLLTALNLPEARPGEAKPATNAASGGGGGGPG"
     gene            complement(467..540)
                     /locus_tag="PLQ11_10830"
     tRNA            complement(467..540)
                     /locus_tag="PLQ11_10830"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(506..508),aa:Gln,seq:ctg)
     gene            1060..1740
                     /locus_tag="PLQ11_10835"
     CDS             1060..1740
                     /locus_tag="PLQ11_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRK25436.1"
                     /translation="MLAQSCRAAKDKGGPLVKVLDHLEEWIITFLIGAATLLIFVAVV
                     HRYGAGASADLTRWATAQGFTGLAAVSGGVFKWLMSIDVSWAQELCIYMFVWMAKFGA
                     AYGVRTGIHVGVDVFINMLNPQDRKRTVLFGLMCGALFTGIIGSLGVYFVYHMYHSGQ
                     ISSDLEMPMWIVYLAIPLGSGLMCFRFLQVAWHFAQTGELPVHDHGAVDGVDDIRADK
                     GLTQEFKS"
     gene            1875..3158
                     /locus_tag="PLQ11_10840"
     CDS             1875..3158
                     /locus_tag="PLQ11_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRK25437.1"
                     /translation="MRAGMVFFLLISMMLTGMPISIALGMTVLIFIFTMTNVPLQSVA
                     LKLFTGIEKFEIMAIPFFILAGNFLTHGGVARRMINFATAMVGHWHGGLGLAAVMACA
                     LFAAVSGSSPATVVAIGSVILPAMVRQGFPPRFGAGVVTTAGALGILIPPSIVMVLYA
                     VSTNTSVGQLFIAGVIPGLVLATMLGITTWYRAWKNGYPRQPKASFGEQIRAFKESAW
                     GLFLIIIVIGGIYTGFFTPTEAAAMSAVYAFIIAVFVYKDMTLKDVPRVLLSSANMSA
                     MLLYIITNAVLFSFVMTNENIPQAMAQWMTDQGFGWITFLLVVNVLLLLAGNVMEPSS
                     IVLILAPILFPVAMKLGIDPVHFGILITVNMEVGMCHPPVGLNLYVASGITRMGITEL
                     TVAVWPWLLTMLVFLVLVTYWPAMSLWLPTVLGMR"
     gene            complement(3370..>4577)
                     /locus_tag="PLQ11_10845"
     CDS             complement(3370..>4577)
                     /locus_tag="PLQ11_10845"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HRK25438.1"
                     /translation="GTVGDIEGLPFFEAIRQLRNDLPEKHAVFVHLTLLPYIPTAGEL
                     KTKPTQHSVQELRSIGIQPDVLLCRCDRPIPDGERRKLALFCNVRPGAVVEAQDTAPL
                     YAVPLAYHRNGLDTEILHAFGIDGAPQPDLTRWERIVETVTNPNGAVTIAVVGKYTEL
                     KDAYKSLIEALAHGGIANRVKVNLDWIDSEVFEGADAAPKLEHVHGILVPGGFGVRGT
                     EGKIAAATFARKNKVPYFGICFGMQMAVIESARNLCGIAGASSSEFGPCEQPVVGLMT
                     EWLSGNQLQKRSEAGDLGGTMRLGAYPAVLKPGSKVAEIYGTTAISERHRHRYEVNMA
                     YRAGLETEGMSFSGTSPDGLLPEIVELNDHPWFIGVQYHPELKSRPFEPHPLFASFIA
                     AAIEQSRLV"
BASE COUNT          788 a         1382 c         1475 g          932 t
ORIGIN      
        1 ccagggccgc cgccgccgcc gccgcttgcg gcattggtcg caggcttggc ttcgcccggc
       61 ctggcctcgg gcaggttaag cgcggtcagc agcatcggct gtgccgactg gggcagggtg
      121 gccacaagct gccgcgccga tgaggcgatc ccgcccggca tccaactcca gaaacccggc
      181 acaaaactgg cggccgccag aatcgtggca aggcaggcaa caacaaggcc ggcaaacttc
      241 ttcatggcgt cccgaacggt gccggacatc agacatcatg cgcccggaca ttggcaccat
      301 acatgacggt tgtcgctggc gtaagccaag cggtgattaa ttattcgtta gcgtaaaaac
      361 ggaggcacaa tcgggcctcc ccgcagcagc gcgccagcaa gcggaccata ccgatgtgcc
      421 gcagtccgtc tggacagcag catcgacaaa cagagcaggg agcgattggc tgggggacct
      481 ggattcgaac caagactaac ggagtcagag tccgctgttc taccgttaaa ctatccccca
      541 ccggtgagcg aagcccttcc ggggagccgc cttctagggc gatttggggg cgcggtcaat
      601 gggaaatgaa cgttgcggtg gggacgtcgc gccgcccttc gtgaaggcgt gattgccagg
      661 gcaatcagga caggccagga ggctccgggg ttatgtcgtt caggatggat agtcaggccg
      721 gtgacgggcc atgacacgcg gcttgtcaga caagccagtt gtatgttgca tgacaagttg
      781 tgagccatgc aaaaattgga tggcagcggt ggcaatcatg gggcttctag tcgggcaagt
      841 cgcgtgatag cgttaacgat gtactagccg aagcagcaag agggcggtga gccgtcggtg
      901 catgaggccg ggcaggggat tgcggcgtgg gcaagaacgc gccgcggtgg ggaggctctt
      961 aggcatgggc cgcgcaaggg ttttgcgccg tcctgggcgt tctcgcatcc tttccggtcc
     1021 gataccgacg cctgaccggc tgttcaggcg atccgatgaa tgctggcgca aagctgccgc
     1081 gcggcaaaag acaagggagg accgcttgtg aaagtgctcg atcatctcga ggagtggatc
     1141 atcacattcc tcataggagc agcgactctg ctcattttcg tcgcggtcgt gcaccgctat
     1201 ggagccggcg catcggctga tctgacccgc tgggcaacgg cgcagggctt caccggcctg
     1261 gctgcggttt ccggcggcgt gttcaagtgg ctgatgtcga tcgatgtctc ctgggcgcag
     1321 gaactgtgca tctacatgtt cgtgtggatg gccaagtttg gcgcggccta tggcgtgcgc
     1381 acgggcatcc atgtcggcgt tgacgtgttc atcaacatgc tcaatccgca ggatcgtaag
     1441 cgcaccgtgc tgttcggcct gatgtgcggc gcccttttca ccggcatcat cggctcgctg
     1501 ggcgtctatt tcgtctatca catgtatcac agcggccaga tctcatcgga tctcgaaatg
     1561 ccgatgtgga tcgtctatct cgccatcccg cttggttcgg ggctgatgtg cttccgcttc
     1621 ctgcaggttg cctggcactt tgcgcagacc ggcgagctgc cggtgcatga tcacggtgcc
     1681 gtcgatggcg tggatgacat ccgtgccgac aagggtctta cgcaggagtt caagtcatga
     1741 gcggtggtgc atttcttacg cccgctgcac cgggcgaacg caagggtctc gggcgggcgc
     1801 tgttcctggc cccgctgttc ttcatcctga tctgcgttgc gcagaaactg ggcattatcg
     1861 aactgccgaa cggcctgcgc gccgggatgg tgttcttcct gctcatctcg atgatgctga
     1921 ccggcatgcc gatctcgatc gcgctgggca tgacggtgct gatcttcatc ttcacgatga
     1981 ccaacgttcc gttgcaatcg gtggcgctga agctgttcac cggcatcgag aagttcgaga
     2041 tcatggcgat cccgttcttc atccttgccg gcaacttcct cacccatggc ggtgtcgccc
     2101 gacgcatgat caatttcgcc acggcgatgg tcggtcactg gcacggcggc ctcgggcttg
     2161 cggcggtcat ggcctgcgcg ctgttcgcgg cggtgtccgg ctcctcgccg gcgaccgtgg
     2221 tggcgatcgg ttcggtgatc ctgccggcga tggtcaggca gggctttccg ccgcgcttcg
     2281 gcgccggtgt ggtgacgacg gccggcgcgc tcggcatcct gatcccgccg tcgatcgtca
     2341 tggtgctcta tgccgtgtcg accaacacat cggtggggca gctgttcatc gcgggcgtca
     2401 ttcccggcct ggtgctggcc acaatgctcg gcatcacaac ctggtatcgc gcctggaaga
     2461 acggctatcc gcgccagccg aaagcctcgt tcggtgagca gatccgtgcg ttcaaggaat
     2521 cggcctgggg tctgttcctg atcatcatcg tcatcggcgg tatctacacc ggcttcttca
     2581 cgccgacgga agcggcggcg atgagcgcgg tctatgcctt catcatcgcg gtgttcgtct
     2641 acaaggacat gacgctgaag gatgtgccgc gcgtgctgct gtcctcggcc aacatgtcgg
     2701 cgatgctgct ctacatcatc accaacgcgg tgctgttctc cttcgtcatg accaacgaga
     2761 acattccgca ggcgatggcg cagtggatga ccgatcaggg cttcggctgg atcaccttcc
     2821 tcctggtggt gaacgtgctg ttgctgctgg ccggtaacgt catggaaccg tcgtcgatcg
     2881 tgctgatcct ggcgccgatc ctgttcccgg tggcgatgaa gctcggcatc gatccggtgc
     2941 atttcggcat cctgatcacc gtcaacatgg aggtgggcat gtgccatccg cccgttgggt
     3001 tgaacctcta cgtggcgtca ggcatcaccc gcatgggcat cacggaactg acggttgccg
     3061 tctggccgtg gctgctgacc atgctggtgt tcctcgtgct ggtcacctac tggccggcga
     3121 tgagcctgtg gctgccgaca gtgctcggca tgcgctgacg ggcacagcga ccgcaacacc
     3181 tgagacgccg ggggcttgcc cccggcgttt tcttttgcgc cgtggcagcg acgtcgcgtc
     3241 aagagaacgg cgaaaataga aaaagacaga gcgcttttgg gtgcatcgga atgcactgga
     3301 aatgctctaa tttcttgttt tatcgcattt tcttgacgcg aaccggactc cacttcgctt
     3361 gaaaatgctc tacaccagcc ggctctgctc gatggctgcg gcaatgaacg aggcgaacag
     3421 cggatgcggc tcgaacgggc gcgacttcag ctccgggtga tactgcacgc cgatgaacca
     3481 cggatggtcg ttgagttcga cgatttccgg cagcagcccg tccggcgatg ttccggaaaa
     3541 gctcatcccc tccgtctcca gcccggcgcg ataggccatg ttgacctcgt agcggtggcg
     3601 gtggcgttcg gagattgcgg tggttccgta gatctccgcc acctttgaac ccggcttgag
     3661 cacggccgga taggcgccaa ggcgcatggt gccgccgaga tcaccggcct cggagcgctt
     3721 ctgcaactgg ttgcccgaca gccattcggt catcaaaccg acaacgggct gctcgcaggg
     3781 gccgaattcg cttgaggagg cccccgcaat gccgcagagg ttgcgggcgc tctcgatcac
     3841 tgccatctgc atgccgaagc agatgccgaa atacggcacc ttgttcttgc gcgcgaacgt
     3901 ggccgccgcg atcttgccct cggtgccgcg cacgccgaag ccgcccggca ccaatatgcc
     3961 atgcacatgt tcgagcttgg gagcggcgtc ggcgccttcg aacacctccg agtcgatcca
     4021 gtcgaggttg accttgaccc ggttggcgat tccgccgtgg gcgagcgcct cgatcagcga
     4081 tttgtaggcg tccttcagct ccgtgtactt gccgaccacg gcgatcgtca ccgcgccgtt
     4141 gggattggtg accgtctcga cgatgcgttc ccagcgcgtc agatccggct gcggcgcgcc
     4201 gtcgatgccg aaggcgtgca ggatttcggt gtcgagcccg ttgcgatgat aggccagcgg
     4261 cacggcatag agcggcgcgg tgtcctgcgc ctccaccacc gcgccgggcc ggacgttgca
     4321 gaacagcgcc agcttgcgcc gctcgccatc cggaatcggt cgatcacaac ggcacagcag
     4381 cacatcgggc tggatgccga tggacctgag ttcctgcacc gaatgctggg tcggcttggt
     4441 cttgagttcg ccggcggtcg ggatgtaagg cagcagcgtc agatgcacga acaccgcatg
     4501 cttttccggc aggtcgttgc gcaactggcg gatcgcctcg aagaacggca ggccttcgat
     4561 gtcgcccacc gtgccgc
//
LOCUS       DAOMXG010000185         4554 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_10169_length_4554_cov_11.299400, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000185 DAOMXG010000000
VERSION     DAOMXG010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4554
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10169_length_4554_cov_11.299400"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..833)
                     /locus_tag="PLQ11_10850"
     CDS             complement(135..833)
                     /locus_tag="PLQ11_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008877408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HRK25439.1"
                     /translation="MSVQLPDNDSDLFSLMRTELFSSVVGDVLDAQGLRHQYLPPNIR
                     PMSDDMVIIGRAMTVLEADFFVEPGMDGRSGLATKPFGVMFEALDDLKANEIYITSGV
                     CGAYAQWGEMMSTRARVLGAAGAILNGYARDTHGIRKLAFPTFCHGSYGQDQGVRGKV
                     LDYRLRIEVGAVTIDPGTILFGDIDGVVAIPKVAEKETLRLAFEKARGEKRVAKAIRE
                     GMSTVEAWNKFGIM"
     gene            complement(847..1908)
                     /locus_tag="PLQ11_10855"
     CDS             complement(847..1908)
                     /locus_tag="PLQ11_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-TA family PLP-dependent enzyme"
                     /protein_id="HRK25440.1"
                     /translation="MTTLAMIETPAVLIDEDIVDRNIAAAQAQFSRLGVALRPHIKTH
                     KLVRFAQKQIAAGAVGITCQKTSEAEAFVEAGCRDILITFNILGDAKLARLRRLSERA
                     TITVVADNAVVVRGLSQAFADAAAPLRVLVECDTGLARCGVQSPEAAVQLGRLIAQSP
                     GLHFAGLMTYPPPNSHEQAETFLREARAGCIASIGHCETVSSGGTPSMKSVPTETVAT
                     EYRPGTYIYNDRSLIAREACKLADCALSVAATVVSRPTPDRAILDAGSKTLSSDLLGL
                     TGYGMLRDYPEAVILGLSEEHGHVDLTACARKPEVGETVLIIPNHACVVTNLFDQVHV
                     HRSGCFVETVHVDARGCVF"
     gene            complement(1911..2306)
                     /locus_tag="PLQ11_10860"
     CDS             complement(1911..2306)
                     /locus_tag="PLQ11_10860"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002965893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRK25441.1"
                     /translation="MTIEHLGGEGFGAARSTMPLSPAVRAGDFVYISGQVPTDDRGEI
                     VWGNVETQTRVVMEKLKVALGYAGCSLSDVVKTTIYLADARDFVGFNGVYSSYFEPGK
                     RPARTTIQSALVLDIRVEIEAVAYAPQKR"
     gene            complement(2337..3275)
                     /locus_tag="PLQ11_10865"
     CDS             complement(2337..3275)
                     /locus_tag="PLQ11_10865"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRK25442.1"
                     /translation="MSEAPQNQDTVQGAEARSGDILLTIRTLLPELSKAHTLIAEMVL
                     ADPNWAVQSNVEDLAARAGVAKPTIVRFARAVGCEGLKDFKLKLAGSLALGSSYLHRS
                     VRTSDTPGEVINNVVGSTLAALAEWHRALDPDLLKQAADALNSARRVDCYGTGATSHF
                     LAQDLQARLFRLGLTATAYTDAYLQLVAAATLGPEDAVVAVSFVGRMQPLLETVGMAK
                     SRGATIIAITRANTPLARLADIVLAVDVPADATMPVGTDAYITQTMMIEMITILLGRL
                     RGPECVKRLEQIHRILQAKDQETDESSVVYWGWKSP"
     gene            complement(3287..4252)
                     /locus_tag="PLQ11_10870"
     CDS             complement(3287..4252)
                     /locus_tag="PLQ11_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRK25443.1"
                     /translation="MSDAPLVRIRGLTKHFGKAPALVHAVEDVSFDIARGEVVGLVGE
                     SGSGKSTIGRLILRALDLTAGTVEFEGREIGRLSQREIRPLRRRMQMVFQDPFASLNP
                     RLRVRDIIGEALDAHGLAQGPARLQRIGELLQIVGLQADHANRFPHEFSGGQRQRIGI
                     ARALAVEPDLIVADEPVSALDVSVQAQVLNLLQDLRRRFGMAMLFISHDLDVIELMCD
                     RIVVLYLGRVMESGPAEEVCRRPRHPYTQALLAASPKPDPDAPRTRRLLQGDIPSPLA
                     PPSGCVFRTRCPLATSDCAQAVPAMRQAGEGHQFACIHAAQAPGH"
     gene            complement(4249..>4554)
                     /gene="dppD"
                     /locus_tag="PLQ11_10875"
     CDS             complement(4249..>4554)
                     /gene="dppD"
                     /locus_tag="PLQ11_10875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01727.1"
                     /note="DppD and DppF are the ATP-binding components of the
                     ABC dipeptide transport system DppABCDF; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein
                     DppD"
                     /protein_id="HRK25444.1"
                     /translation="VTVMYAGEVVESADVRTLFHRPRHPYTHALINCLPARAEPGPDG
                     RRLVRSIPGTVASAAGATGCKFAPRCAHAAPPCHAAHPALESVEDGQTVRCCRWRDL"
BASE COUNT          870 a         1420 c         1450 g          814 t
ORIGIN      
        1 tgccgagggt acggatgtct gatcaacgca ggttcggcta acggcaatgt tggccgaact
       61 ttttgtgcgt tttcagcaag tagcgttgct attgccgaga tcatctgcga gacgtccggc
      121 ggcgtgttcg aagtctacat aatcccgaac ttgttccagg cctcgacggt gctcatacct
      181 tcccgaatgg ctttggctac acgcttttcg ccgcgcgcct tttcaaacgc aagccgcaag
      241 gtttccttct ccgccacttt gggaatggcg acaacaccgt cgatgtcgcc gaacaggata
      301 gtgcccggat cgatcgtcac cgcaccgact tcaatccgca gccgatagtc gagcaccttg
      361 ccgcgcacgc cctgatcctg cccataggag ccgtgacaga aggttggaaa ggcgagcttg
      421 cggatgccat gggtgtcgcg ggcataaccg ttcaggattg ccccggccgc gccgagaacg
      481 cgggcgcggg tcgacatcat ctcaccccac tgggcatagg caccgcagac gccggaggtg
      541 atgtagatct cgttcgcctt caaatcatca agcgcttcga acatcacgcc gaagggtttg
      601 gtcgccagac cggaccgccc gtccattccc ggctcgacaa agaagtctgc ctccagaacg
      661 gtcatggcgc gaccgatgat caccatgtca tcggacattg gccggatgtt gggcggcaga
      721 tactggtgcc ggaggccctg cgcatcgagc acatcgccga ccacgctgga aaaaagctcc
      781 gtgcgcatca gggaaaaaag atcggaatca ttgtcgggaa gctggacaga catcggtgtt
      841 cctctttcag aaaacgcagc cgcgcgcatc gacgtgcacg gtttcaacga agcacccgct
      901 acggtggaca tgaacctgat cgaacaggtt ggtgacgacg caggcgtgat tgggaatgat
      961 caggaccgtc tcgccgacct ccggtttgcg ggcgcaggcc gtcaggtcca catggccatg
     1021 ctcctcactc aaccctaaaa tcaccgcctc gggataatcg cgcagcatgc catagccggt
     1081 caggcccaga agatcggaac tcagggtctt ggaccctgca tccaggatcg cccggtccgg
     1141 tgtcgggcgg ctgacaaccg tcgctgcaac gctgagcgcg caatcggcaa gcttgcaggc
     1201 ctcgcgcgcg atcagcgagc ggtcgttgta gatgtaggtg ccggggcgat attcggttgc
     1261 gacggtctct gtcggaactg acttcatcga cggcgtgcca cccgaactga cggtctcgca
     1321 atgcccgatg ctagcaatgc agcccgccct ggcctcgcgc aggaaggtct cggcctgctc
     1381 atggctgttg gggggcggat aagtcatcaa cccggcaaag tgcaatcccg ggctttgcgc
     1441 gatcagacgc ccgagctgca ccgcagcttc agggctttgc acaccgcaac gggcgagccc
     1501 ggtgtcgcat tcgacaagta cgcgcagggg ggcagcggca tccgcaaacg cctgtgacaa
     1561 tccgcgaacg accacagcgt tatcagcaac gaccgtaatg gtcgcccgtt cgctcaggcg
     1621 gcgaaggcgc gccagcttgg catcgccgag gatgttgaag gtgatcagaa tatccctgca
     1681 tcccgcttcg acaaaggctt cggcctccga tgtcttctgg caggtgatgc caacagcgcc
     1741 ggcggcgatc tgcttttggg cgaaccgcac cagcttgtgg gtcttgatgt gcgggcgcag
     1801 tgcaacgccg agacggctga actgtgcctg tgccgccgcg atgttgcgat cgacgatatc
     1861 ttcgtcaatc aacaccgcag gggtttcaat catggcaagg gtggtcatgg tcagcgcttc
     1921 tgaggggcat aggccacggc ctcgatttcg accctgatgt cgagaacgag tgccgactgg
     1981 atggtggtgc gcgccggacg tttgccgggt tcgaaatagc tggaatagac accgttgaag
     2041 ccgacgaaat cgcgcgcatc agccagataa atcgtagtct tcacgacatc agagagcgaa
     2101 cagccggcat atccgagtgc gactttcagc ttttccatga ccacgcgggt ctgggtctcg
     2161 acattgcccc agacaatttc cccgcgatca tccgtgggca cctggccgga gatatagaca
     2221 aagtcgccgg cgcggacggc aggcgacaac ggcatggtgc tgcgcgcggc gccaaaaccc
     2281 tctccgccaa ggtgttcaat cgtcatttct ttgtctccaa cagcggtttc aacgtttcac
     2341 gggcttttcc agccccagta aacgacggaa ctttcgtccg tttcctgatc cttggcctgc
     2401 aggatcctgt ggatctgctc cagccgtttc acgcactccg gcccgcgcag ccggcccagc
     2461 aggatggtga tcatctcgat catcatggtc tgggtaatat aggcgtccgt gccgacgggc
     2521 atggtcgcat cagccggcac atccacggcc agaacgatat cggcgaggcg ggccagcggc
     2581 gtgttcgcac gggtgatggc gatgatggtc gcgccccgcg atttcgccat gccgacggtt
     2641 tcgagcaaag gctgcatgcg cccgacgaag gaaaccgcaa cgacggcatc ctcagggcca
     2701 agcgtcgctg cggcgaccag ctgaaggtag gcatcggtgt aggctgttgc cgtcagcccc
     2761 agccggaaca ggcgggcctg caggtcttga gccagaaaat gtgatgtcgc gccggtgccg
     2821 tagcaatcga cccggcgtgc gctgttcaat gcatcggcgg cctgcttcag caggtcgggg
     2881 tcgagcgcgc ggtgccattc cgctagcgcc gccagggtag agccgacgac gttgttgatc
     2941 acttcgcccg gcgtgtccga ggtccgcacg gagcggtgca gataggagga gccaagtgcc
     3001 agcgatccgg ccagcttcag cttgaaatcc ttcaatccct cgcaaccgac agcgcgggca
     3061 aagcgcacga tggtgggctt tgccacgccg gcccgcgccg ccagatcttc gacattgctc
     3121 tgcacggccc agttcgggtc cgccagaacc atctcggcga tcagagtgtg tgcttttgaa
     3181 agctccggca gcagcgtgcg gatggtcagg agaatgtcgc cggatcgcgc ctcagcgccc
     3241 tgaacggtgt cctgattttg tggggcttcg ctcatcgccg cgtccttcag tgacccggtg
     3301 cctgggcggc gtggatgcag gcaaactggt gcccctcgcc agcctgtctc atggccggca
     3361 cggcttgggc gcaatcggat gtcgcaaggg ggcagcgcgt gcggaacacg cagcccgagg
     3421 gcggggcgag cggcgaggga atatcgccct gcagcaatcg acgggtccgc ggggcatctg
     3481 gatcgggctt gggggacgcg gccagcagcg cctgcgtata gggatgtcgc gggcggcggc
     3541 agacttcctc cgccgggcca ctttccatga cacggccgag atagagcacg acgatccggt
     3601 cgcacatcag ctcgatcaca tcgagatcat ggctgatgaa cagcatggcc atgccgaagc
     3661 gccggcgcag gtcctgcagc agattcagca cttgcgcctg taccgacaca tcgagggcgg
     3721 aaaccggttc atcagcgacg atcagatctg gttcgactgc cagcgcgcgg gcaatgccga
     3781 tacgctggcg ctggccgccg gaaaactcgt gcggaaaccg gttggcgtga tctgcctgca
     3841 gtccgacaat ctgcagcaat tcgccgatgc gctgcaaccg cgccggacct tgcgcaaggc
     3901 catgggcatc aagcgcctca ccgatgatgt ccctcaccct gaggcgcgga ttgaggctgg
     3961 cgaacgggtc ctgaaacacc atctgcatcc gccggcgcaa tggacggatc tcgcgctggc
     4021 tcagcctgcc gatctcgcgt ccctcgaact ccaccgtgcc ggcagtcaga tcaagggcgc
     4081 gcaggatcag gcgaccgatg gtgctcttgc cggagccgga ttcgccgaca agcccaacca
     4141 cctcgccgcg cgcgatatcg aagctgacgt cctcgacggc atgcaccagc gccggggcct
     4201 tgccgaagtg cttggtcagg ccacgaatac ggaccagcgg ggcgtcgctc acaggtccct
     4261 ccagcggcag cagcggacgg tctgcccgtc ttcgacggat tccagagccg gatgggcggc
     4321 atggcaaggc ggcgcggcat gggcgcagcg cggcgcgaat ttgcatcccg tggcgcccgc
     4381 cgcgctggcg accgtgccgg gaatcgagcg gacgaggcgg cgtccatccg gccccggttc
     4441 ggcgcgggcc ggcaggcaat tgatcagcgc atgggtgtag ggatggcgcg gccgatggaa
     4501 cagggtgcgg acatcggccg attccaccac ctcgccggca tacatcaccg tgac
//
LOCUS       DAOMXG010000186         4351 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_10860_length_4351_cov_11.793063, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000186 DAOMXG010000000
VERSION     DAOMXG010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4351
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10860_length_4351_cov_11.793063"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="PLQ11_10880"
     CDS             <1..388
                     /locus_tag="PLQ11_10880"
                     /inference="COORDINATES: protein motif:HMM:NF017083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HRK25445.1"
                     /translation="FEQESKGENLLPVNFLRQMIRFYGDSMQGLVPRYLDLSMDRLMQ
                     EQQNFREQMQKTFGTFPLGNVEEQVRANMAMFNEALRMFSPFQAGKQASAGGEPVADP
                     AREDEVSSLKRELNAMKARLDKLDKR"
     gene            512..835
                     /locus_tag="PLQ11_10885"
     CDS             512..835
                     /locus_tag="PLQ11_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1476 domain-containing protein"
                     /protein_id="HRK25446.1"
                     /translation="MTTFDKREDTAEKKFAHDEELMFKASARRNKLLGLWAAEKLGKT
                     GAEADAYAKSVVMADFAEAGDDDVLRKVKADLDAANVSQSEHQIRRTMDELLARAIAE
                     VKSGA"
     gene            956..1756
                     /locus_tag="PLQ11_10890"
     CDS             956..1756
                     /locus_tag="PLQ11_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016557881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HRK25447.1"
                     /translation="MTHPLLTYADRLRAGEATFTAWTALGSPASAEVLAREAFDSVTF
                     DMQHSLNDANSVIQGISLAALAGKPSLVRIALEDFSFASRALDYGAVGVIAPMINSAA
                     DARRLVAATKYPPLGERSWGPIRSLPLFGLEAPAYLSIANRATVAVAMIETQAALDNL
                     EAILAVEGIDGVFVGPSDLSIALFKGSRIDQLAPEVDAALKHVVARAKAHGKFAMAFT
                     ASGARAAEHARMGYVMMSVAYEVLLLQKGARAELAAAQAASGKGPVTY"
     gene            complement(1880..3112)
                     /locus_tag="PLQ11_10895"
     CDS             complement(1880..3112)
                     /locus_tag="PLQ11_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRK25448.1"
                     /translation="MTLKDAIVSSVDALRANALRSILTMLGIVIGVAAVIAMMAVGSG
                     AREQVNQQIRSLGSNLIQIVPGNVTSGGVRMGSGQSSTLTDDDARAIVAEIPSVQIAA
                     PILRGTGQIVAGGTNWSTGILGVTPDFFEAREWAITSGREFSPDEASRGGQIILLGQT
                     VKRQLFGEDDPIGQAVRVRNVPFEVIGVMDRKGQTVFGTDQDDIVFVPLPTARQRVVG
                     ANRAKNRSVSQVMIKVREGEDLGAVEREVKSLLRQRHRLREDADDDFQIRNLAEISAT
                     REQSARTLALLLAAVAGVSLVVGGIGIMNIMLVSVTERTREIGVRMAVGARPLDILCQ
                     FLIEATTLSVIGGLIGIAIGAGVAKVSADAAGWPLLIEPQVVLLAVVFSGLVGIASGL
                     YPAVRASRLDPVEALRAG"
     gene            complement(3109..3840)
                     /locus_tag="PLQ11_10900"
     CDS             complement(3109..3840)
                     /locus_tag="PLQ11_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRK25449.1"
                     /translation="MTALVEIEGVSRTYRMGTEEVRALIDVSLSISEGEFVAVMGPSG
                     SGKSTFMNVIGCLDRPSAGLYRLAGETVTGLAPSALASVRNRRIGFVFQQFNLLQRAD
                     ALANVELPMIYAGIAPAERHRRARAALERVGLGDRLHHRPLELSGGQQQRVAIARALV
                     NDPKVLLADEPTGALDSRTSLDIMALFTELNRQGMTVIVVTHEEEIARYVRRLIRFRD
                     GRVVADTVQQPADVAAALAAWRDAA"
     gene            complement(3844..>4351)
                     /locus_tag="PLQ11_10905"
     CDS             complement(3844..>4351)
                     /locus_tag="PLQ11_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25450.1"
                     /translation="AGSGRGGRGGGPGAFIERLKGDLGLTDDQKQAVDKILAELPARG
                     GGQGRGMGGMGGMGGTGGGSGGGQARTQLLERIKPILNEQQQAKLTEIAEAGRSGRQR
                     GAGQAVVTGRVYVLNEKGEATGQTVRLGASDGAFTEIISGLAAGASVVVGGGPSAARP
                     ASGFRFGM"
BASE COUNT          757 a         1403 c         1440 g          751 t
ORIGIN      
        1 cttcgaacag gaaagcaagg gcgaaaacct gctgccggtc aatttcctca ggcagatgat
       61 ccgcttctac ggcgacagca tgcaggggct ggtgccccgc tatctcgatc tgtcgatgga
      121 ccggctgatg caggagcagc agaatttccg cgagcagatg cagaagacat tcggcacatt
      181 tccgcttggc aatgtcgagg aacaggttcg cgccaacatg gcgatgttca acgaggcgct
      241 gcggatgttc tcgccgttcc aggccggcaa gcaggccagc gccggtggcg agcctgtagc
      301 ggatcctgcc cgggaggacg aagtgtccag cctcaagcgc gaactcaacg cgatgaaggc
      361 gcggctcgac aagctcgaca agcgctgagc cggccggttt ctgccaggct gcctcgttct
      421 ttcgttcggg gccgttgatt tggcgggcgc ggatgcgtat ctgaggcgtc ggaaagcggc
      481 ggttcgccgc gccgcgacaa agggtgtgat catgacgacg ttcgacaagc gcgaagatac
      541 cgccgagaag aaattcgccc acgacgagga gctgatgttc aaggcatcgg cccggcgcaa
      601 caagctgctc ggcctttggg ccgccgaaaa gctgggcaag actggtgccg aggccgatgc
      661 ctatgccaag agcgtggtga tggccgattt tgccgaagcc ggcgatgatg acgtgctgcg
      721 caaggtcaag gccgatctcg acgctgccaa tgtcagccag agcgagcacc agatccgccg
      781 caccatggat gaactgctgg cgcgcgccat cgccgaggtg aagagcggcg cctgagcgga
      841 agggcggcgg cacggccgat cttgcctgtc atcagacaag tgcttgcctt gccatgcctt
      901 tggctggcat gatgcgggcg ccgggcttcc ggcgcccttt ttgctggaac ctgccatgac
      961 ccatccgctg ctgacctatg ccgatcgcct gcgtgcgggc gaagccacct tcacggcatg
     1021 gacggctttg ggttcgccag cttcggccga agttctggcg cgcgaagcgt ttgattcggt
     1081 caccttcgac atgcagcatt cgctgaatga tgccaacagc gtgatccagg gcatttccct
     1141 ggcggcgctg gccggcaagc cgtcgcttgt ccgcatcgcg cttgaggatt tttcctttgc
     1201 ctcccgggcg ctcgactatg gagcggtcgg tgtcatcgcg ccgatgatca attcggccgc
     1261 cgatgcgcgg cggctggtgg cggccaccaa atatccgccg ctgggcgagc ggtcgtgggg
     1321 gccgatccgc agcctgccgc tgttcggtct tgaggcgccg gcctatcttt cgatcgccaa
     1381 ccgggccact gtggctgtgg ccatgatcga gacccaggcg gcgcttgaca acctggaggc
     1441 gattctcgcc gtcgagggta tcgacggggt gtttgtcggt ccttcggatc tttccatcgc
     1501 attgttcaag gggagccgga tcgatcagct cgctccggag gttgatgcgg cgctcaagca
     1561 tgtcgttgcg cgggccaagg cgcatggcaa gttcgccatg gctttcaccg cgtcgggtgc
     1621 acgggccgcc gagcatgccc ggatggggta tgtcatgatg tcggtcgcct acgaagtgct
     1681 gctgttgcag aagggtgcac gggcggagct ggccgcagcg caggccgcca gcggcaaggg
     1741 cccggtgacc tattgaggtt agggcatttt caagcgaagt ggagtccggt tcgcgtgaag
     1801 aaaatgcgac aagacaagat gtaacttatt gataccgatc acgccgcatt ttgatgattc
     1861 catcaaaatg cggcgtgatc tagccggcgc gcaaggcttc gaccggatcg agacgcgagg
     1921 cgcgcaccgc cggatagagc ccggaggcga tgccaaccag gccggagaaa accacagcca
     1981 gcaggacaac ctggggctcg atcagcagcg gccagccggc tgcatccgcc gagaccttgg
     2041 caacgccggc gccgatggcg atgccaatga ggccgccgat caccgacagg gtggtcgcct
     2101 cgatcaggaa ctggcacagg atgtcgagcg gtcgggctcc taccgccatg cgcacgccga
     2161 tttcgcgggt ccgttcggtc accgaaacca gcatgatgtt catgatgccg atgccgccga
     2221 caaccagcga aaccccggcg accgccgcca gcagcagtgc caaggtgcgg gcgctctgtt
     2281 cgcgtgtggc gctgatctcg gcaaggttgc ggatctggaa atcgtcatcg gcgtcctcgc
     2341 gcagccgatg gcgctggcgc agcagggact tgacctcgcg ctcgaccgcg ccgaggtcct
     2401 cgccctcgcg caccttgatc atgacctggc tgaccgagcg gttcttggcg cggttggcgc
     2461 cgaccacgcg ctgccgtgct gtcggcaacg gcacgaagac gatgtcatcc tgatcggtgc
     2521 cgaacaccgt ctggcccttg cggtccatca cgccgatgac ctcgaacggc acgttgcgca
     2581 cccgaaccgc ctggccgatc ggatcatcct cgccgaacaa ctgccgcttg actgtctggc
     2641 caagcaggat gatctggccg ccgcggctgg cctcgtcagg cgaaaattcc cgcccgctgg
     2701 tgatcgccca ttcgcgggcc tcgaagaaat ccggcgtcac cccgagaatt ccggtcgacc
     2761 agttggtgcc gccggcgacg atctggccag tgccgcgcag gatcggcgcg gcaatctgca
     2821 ccgacgggat ttcggcgaca atcgcccggg cgtcatcgtc ggtcagcgtc gaggactggc
     2881 cgctgcccat gcgcacgccg ccggaggtga cgttgccggg cacgatctga atgaggttgg
     2941 agccgagcga acggatctgc tggttgacct gttcgcgcgc gcctgatccg accgccatca
     3001 tcgcgatgac cgctgccacg ccgatgacaa tgccaagcat ggtcaggatc gaacgcagcg
     3061 cattggcgcg cagcgcatcg acagaggaaa cgatcgcgtc cttgagtgtc atgcggcatc
     3121 ccgccaggcg gcaagggcag ccgccacatc tgccggctgc tgaaccgtgt cggcgacgac
     3181 gcggccatcg cgaaagcgga tcaggcggcg cacgtagcgg gcgatctcct cctcgtgggt
     3241 caccacgatc accgtcatgc cctgccggtt cagctcggtg aacagcgcca tgatgtcgag
     3301 cgaggtgcgg ctgtcgagtg cgcccgtggg ctcgtcggcc agcagcacct tgggatcatt
     3361 gaccagggca cgggcaatgg cgacgcgctg ctgctgaccg ccggagagct ccagcggacg
     3421 atgatgcagc cggtcgccaa gcccgacccg ctccagagcg gcccgtgcgc ggcggtgccg
     3481 ttcggcaggg gctatgccgg catagatcat cggcagttcg acattggcca gggcatcggc
     3541 ccgctgcagc agattgaact gctggaaaac aaaaccgatc cgccggttgc gcacgctggc
     3601 gagcgccgac ggagcaaggc cggtgaccgt ttcgcccgcc agccggtaca gacccgcgct
     3661 tggccggtcg aggcagccga tcacgttcat gaatgtcgac ttgcccgagc cggatggccc
     3721 catcacggcg acgaactcgc cctcgctgat ggaaagcgag acatcgatca gagcacgcac
     3781 ttcctcggtg cccatccgat aggtgcggct gaccccctcg atctcgacga gcgcggtcat
     3841 ggttcacatc ccgaaacgga agccgctggc aggacgggcg gcgctggggc cgccgccgac
     3901 caccaccgag gcgcccgctg caagaccgga gatgatttcg gtgaatgcac cgtcgctggc
     3961 tcccagccgg acggtctgtc ccgttgcctc gcctttttca ttgagcacgt agacgcggcc
     4021 ggtaacgacc gcttgtccgg ccccgcgctg acggcctgag cggccggcct cggcgatctc
     4081 ggtcagcttt gcctgctgtt gctcgttgag gatcggcttg attcgctcca gcagctgggt
     4141 gcgggcctgc cctccgccgc tgccgccgcc ggttccaccc atgccgccca tgccacccat
     4201 tccgcgcccc tgaccgccgc cgcgtgcggg aagttcggcg aggatcttgt cgaccgcctg
     4261 tttctgatcg tcagtaagcc cgagatcacc cttgaggcgc tcgatgaagg cgcccggtcc
     4321 accgccgcgc ccgccgcggc cggagccggc c
//
LOCUS       DAOMXG010000187         4332 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_10922_length_4332_cov_8.368483, whole genome shotgun sequence.
ACCESSION   DAOMXG010000187 DAOMXG010000000
VERSION     DAOMXG010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4332
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10922_length_4332_cov_8.368483"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..725)
                     /locus_tag="PLQ11_10910"
     CDS             complement(126..725)
                     /locus_tag="PLQ11_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25451.1"
                     /translation="MSVTAPSTIGETLRGLVRQALKLFGGVLALGFVALFSDDPLLTF
                     FKMLSIAGTVLMLMSVALMLVTFRRARVLAPAALLTTLAVSLASTLISLWLGGGVVDP
                     TRGMAALASGGLIGWAWASTMLLFVDEGVVRGRGTLWHLAIWAVTFAINQLAAVTGQG
                     DPGIVQIVMLASAGIAAGHAVGLSLRIRSARALALHQEG"
     gene            989..1666
                     /locus_tag="PLQ11_10915"
     CDS             989..1666
                     /locus_tag="PLQ11_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRK25452.1"
                     /translation="MTVRLKHKTLSAAIVDQLRLAILNGEHKAGAQLRQDALAEVYGV
                     SRIPVREALFQLEAEGLVRIVPHKGAVVSELSLEEINDVFDLRGILEPRLLAHSAPLL
                     DAADFTRLDQVQARFVAAIAQRNVRDFGALNAEFHLALYIRAPQPRTQAIVAGLLQIS
                     DRYTRLQLSNAAAMETAASEHASLVSLCRAGRFDEASRFLLRHIETVRSGLLRVAGRA
                     LTGRAAG"
     gene            1788..3500
                     /locus_tag="PLQ11_10920"
     CDS             1788..3500
                     /locus_tag="PLQ11_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HRK25453.1"
                     /translation="MSQESPPETPRSATGRLTAWLAGWGWPDIALLAPHGVALAFMLG
                     TEKPVVPMLAFVLGWVFFNAFWIVLIRRPAVAGLLTLLCLLLLILLSQFKHEILLMTV
                     NFVDLMMIDADTTAFLLQVFPRLGFKTVLAALAAVALLALVWWLDRRRLSRRCAALVA
                     TGSFAALISLASAVPNDPWEEFYAENYFSKYVRSGVTAASDYLTRGILDQDQGAVARL
                     ATPDPGPCTVEQPPHVIVIHDESSFDARAIPGIKVPEGYGGHFRSFDGKARTLLVEGP
                     GGPSWYTEYNILTGLSARSYGRFIDFATRIAAGRVERGFAHNFARCGYRTTTLYPMYG
                     SFLSARRFQETAGIQRFLDSKVLGAGFLDPDGFYFNAAADLLARERRLGPQFMLVYTA
                     QNHFPWTFRYRPDLAPEWKDPGNRADVDEYLRRQHLTEVDYRAFIERLQREFPEERFL
                     IVRFGDHQPYFARHLIDPTQDDTMLVRRIAAADPRFLATYYSIETINFTPKDLSSSLD
                     VLDAPHLPLAVLEAAGVPLDPTFREQKAILQRCRGLLFRCNAGAEARRFNRQLIDAGL
                     IKGL"
     gene            3731..>4332
                     /locus_tag="PLQ11_10925"
     CDS             3731..>4332
                     /locus_tag="PLQ11_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HRK25454.1"
                     /translation="MPNEIVVGSNRASLYDIAEKYHVSMSDLYALNPQFQRGTTDGKF
                     NAALEKQKIASGAGKRDGDYIEQGELIKLPDAAPSGPFYFYKSEASTYQREAVLKAVD
                     KVRKATPDGQGALTEEQFVAIATRVKGVSEKEARDLFNELAQETISVADGPATISARE
                     VIDARASAVRTADNERAKEARAEEQRRQAAASRVSPASEPG"
BASE COUNT          769 a         1381 c         1399 g          783 t
ORIGIN      
        1 ggcgtgaccg ccgcgcgggg cgccggctgc ggcgacgccg gagcggctat cgctatgccg
       61 cactgtgtgc agaaccggtc gttctcggcc agtttggcgc cgcactgggt gcaaaagccc
      121 atggatcagc cctcctgatg aagtgccagc gcgcgtgcgc tgcggatacg caggctcagg
      181 ccgaccgcat gaccggccgc gatcccggcg ctggcgagca tgacgatctg gacgatgccc
      241 ggatcgccct ggccggtcac ggcggccagt tggttgatgg cgaaggtcac ggcccagatg
      301 gccagatgcc agagggtgcc gcggccgcgc acaacgccct cgtcaacgaa cagcagcatc
      361 gtcgaggccc aggcccagcc gatcaatccg cctgaggcca gggccgccat gccgcgcgtg
      421 ggatcgacca cgccgccgcc gagccacagg ctgatcagcg tgctggcaag gctgacggcc
      481 agcgtcgtca gcagcgccgc cggcgccagc acgcgggcgc ggcgaaaggt caccagcatc
      541 agcgcgaccg acatcagcat cagcaccgtg ccggcgatcg acagcatctt gaagaacgtc
      601 agaagcggat cgtcgctgaa cagggccaca aagcccaatg ccagcacccc gccgaacagc
      661 ttcagcgcct gccggacaag gccacgcagc gtttcgccaa tggtggaagg cgcggtaacg
      721 gacacgggca gcctcttgat acaggcgaca ttgcagcgca ggatcgcagc tgccgcaatg
      781 gcataggtca atgctccgga atcggcaccg ggcggcgccg gcctgtcggc cggcaagctt
      841 gccgcgcggc gcccttgtcg ggggcaggca ccttctctag aacggagcgg ttgaccacgc
      901 agagcgccca tcggcgacgc atccggctgc ccttgcatgg tcgccaagca aaccggtggc
      961 ggattcttgt tcgggagcag atgccgcaat gactgtgcgc ctcaagcaca agaccctgtc
     1021 tgcggcgatc gttgaccagc tgcgcctggc aatcctcaat ggcgagcaca aggcgggggc
     1081 gcagttgcgc caggatgccc tggccgaggt ctatggcgtc agccgcattc cggtgcgcga
     1141 ggcgctgttc cagcttgagg cggagggcct ggtgcgcatc gtgccgcaca agggcgccgt
     1201 ggtctccgaa ctgtcgcttg aagagatcaa cgatgtcttt gatctgagag ggattctgga
     1261 gccgcgcctg ctggcgcatt cggcccccct gcttgacgcg gccgatttca cccgcctcga
     1321 tcaggttcag gcgcggttcg tcgcggccat cgcccagcgc aatgtgcgcg attttggcgc
     1381 gctgaacgcc gaattccacc tggccctcta catacgcgct ccccagcccc gcacgcaggc
     1441 gatcgtcgcc gggcttttgc agatatccga tcgatatacg cgcctgcaat tgtcgaatgc
     1501 cgcggccatg gaaaccgccg ccagcgaaca tgcaagcctc gtctcgctgt gtcgggcagg
     1561 gcggtttgac gaggcaagcc gctttctgct gcggcatatc gagacagtgc ggagcggcct
     1621 gctgcgggtg gccggtcggg ccttgacggg gcgggctgcg ggctaaatca ttttcaagcg
     1681 aagtggattc cggttcgttg aaaacggctt tagccggctt ccatcaagac gcggcatgca
     1741 ctaacactct ggagcatgat ggtccgcaga cgggaggatc gagcgcgttg agccaggaaa
     1801 gtccacctga aacgccacga tcagcaaccg ggcgcctgac ggcctggttg gcgggatggg
     1861 gctggccgga catcgcgctg ctggcgccgc atggcgtcgc gcttgcattc atgctcggca
     1921 cggaaaagcc cgttgtgccg atgctggcct tcgtgttggg ttgggtgttc ttcaacgctt
     1981 tctggatcgt gctgatccgc cgcccggcgg ttgccggcct gttgacgctg ctctgcctgc
     2041 tgctgctgat cctgctgtcg cagttcaagc atgaaatcct gctgatgacc gtcaatttcg
     2101 ttgatctgat gatgattgac gccgatacga cggcttttct gctccaggtg tttccacggc
     2161 tcggcttcaa gaccgtgctg gcagcgctgg cggctgtggc gctcttggcg cttgtctggt
     2221 ggcttgatcg gcggcgcctg tcgcgacggt gcgcggcgct ggtcgccacc ggctcgtttg
     2281 cggcgctgat ctcgctggcc tcggcggtgc cgaacgatcc gtgggaagag ttctatgccg
     2341 agaactactt ctcgaaatat gtgcgctcgg gcgtcacggc ggccagcgac tatctgaccc
     2401 gcggcattct cgatcaggat cagggggcgg tcgcccgcct tgccacgcct gatcccggac
     2461 cctgcacggt cgagcaaccg ccccatgtga tcgtcattca tgacgagtcg agcttcgatg
     2521 cccgcgccat ccccggcatc aaggtgcccg agggatacgg cgggcatttc agatccttcg
     2581 acggcaaggc gcgcacgctg ctggtcgagg ggccgggcgg gccaagctgg tataccgaat
     2641 acaacatcct gaccgggctt tcggcgcgct cctatggccg tttcatcgac tttgccacgc
     2701 gcattgcagc cgggcgggtc gagcgcggct ttgcccataa tttcgcccgc tgcggctacc
     2761 ggaccacgac gctctatccg atgtatggct cgttcctgtc ggcgcggcgc tttcaggaga
     2821 ccgccggcat ccagcgcttt cttgacagca aggtgctcgg cgccggcttt cttgacccgg
     2881 acgggttcta tttcaacgcc gctgccgatc ttttggcgcg tgagcggcga ctgggtccgc
     2941 agttcatgct ggtctacacc gcccagaacc attttccctg gacgttccgc taccggcccg
     3001 accttgcgcc cgagtggaag gacccgggca atcgcgccga tgttgatgaa tatctgcgcc
     3061 gccagcacct gaccgaggtg gattaccgcg ccttcatcga acggctgcaa cgcgagtttc
     3121 ctgaagagcg cttcctgatc gtccgcttcg gcgaccatca gccgtatttc gcccgccacc
     3181 tgatcgaccc gacgcaggat gacacgatgc tggtgcggcg gatcgcggcg gccgacccgc
     3241 gatttcttgc cacctattac tccatcgaga ccatcaattt tacccctaag gatctgtcct
     3301 cgagcctcga tgtgctcgat gcgccgcatc tgccgctggc tgtgctggag gcggcgggcg
     3361 tgccgctcga tcccaccttc cgcgaacaga aggcaatcct ccagcgctgc cgcgggctcc
     3421 tgttccgctg caacgccggc gccgaggcaa gacggttcaa tcgccagctc atcgatgccg
     3481 gcctgatcaa agggctgtaa gatcgctcgc ttccgccggg cggcgatatg gcgcgcaaag
     3541 gctggcgggc gacggccgcc gcccccgccg agcaacaact gttgacaaaa cgtaaaattt
     3601 aataaaaata cgcaaaaatt gccttgtgaa cgaacgaccg cgacatcctg cccgcgtggc
     3661 gatcggtccg tcggggaaca tggctggacg cgcggttcgc ccgccagcgc tgttatgaag
     3721 tgagggaatg atgccaaacg agattgttgt cggaagcaac cgcgcatcgc tgtatgacat
     3781 cgctgagaaa tatcatgttt cgatgagcga tctttatgcg ctgaaccctc agttccagcg
     3841 tggaaccact gatggcaagt tcaatgcggc gcttgaaaag cagaagatcg catcgggcgc
     3901 gggcaagcgc gacggcgatt acattgaaca gggcgagttg atcaagctgc ccgacgctgc
     3961 accatccggt ccattctatt tctacaagag tgaggcctcg acctatcagc gcgaggccgt
     4021 gctcaaggcc gtcgacaagg tccgcaaggc gacgccggac gggcagggag cgctcaccga
     4081 ggagcagttt gtcgcaattg ccaccagggt gaagggcgtt tccgaaaagg aagcgcggga
     4141 cctgttcaac gaactggcgc aggaaacgat ttccgtggcc gacggtcctg cgacgatcag
     4201 cgcccgcgag gtcattgatg ccagggccag tgccgtcagg actgcggaca acgaacgcgc
     4261 caaagaggcc cgggccgagg agcagcgtcg gcaggcggcc gccagccggg tgtctccggc
     4321 ttccgaaccc gg
//
LOCUS       DAOMXG010000188         4287 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_11076_length_4287_cov_11.146267, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000188 DAOMXG010000000
VERSION     DAOMXG010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4287
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11076_length_4287_cov_11.146267"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="PLQ11_10930"
     CDS             <1..388
                     /locus_tag="PLQ11_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HRK25455.1"
                     /translation="SQPGTPLPSPTELDRRFAKNAPIWSLNGGASNGFSGRPLFSVRR
                     GTPVVLAITNRTHWSQVLTVRGHVFRLLHPLDDGWEPYFLDTVHLPDNSVSRIAFDAV
                     NPGRWAIRSTIAEHYDAGVYTWFEVA"
     gene            complement(402..1874)
                     /gene="gatB"
                     /locus_tag="PLQ11_10935"
     CDS             complement(402..1874)
                     /gene="gatB"
                     /locus_tag="PLQ11_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017486316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HRK25456.1"
                     /translation="MSVAVNPKKLIKGATGDWEIVIGMEIHAQVTSKSKLFSGASTAF
                     GGEPNSHVSLVDAAMPGMLPVINAECVAQAIRTGLGLKAEINLKSRFDRKNYFYPDLP
                     QGYQISQYKHPIVGEGEVIVDVPGEAEPIVVGIERLHLEQDAGKSIHDQDPNNSFVDL
                     NRSGVALMEIVSRPDLRSAEEAQAYVSKLRTILRYLGTCDGDMEKGNLRADVNVSVRK
                     PGDKLGTRCEIKNVNSIRFIGQAIEVEARRQIGILEDGGSIDQETRLYDPGRRETRSM
                     RSKEEAHDYRYFPDPDLLPLEFPQDYVDSLKAALPELPDEKKARFISQYGLSAYDASV
                     LIAERESADFFEAAAKGRDGKAVANWTINELFGRLNKDGKGIADSPVNAAQLGGIVDL
                     IASGAISGKIAKDVFEIVYTEGGDPAGIVEKRGLKQVTDTGAIEKVVDEIIAANPDKV
                     AQAKEKPTLLGWFVGQVMKASGGKANPGAVNELLKSKLGI"
     gene            2081..2578
                     /gene="msrA"
                     /locus_tag="PLQ11_10940"
     CDS             2081..2578
                     /gene="msrA"
                     /locus_tag="PLQ11_10940"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015242026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HRK25457.1"
                     /translation="MSDKSERAVLAGGCFWGMQDLFRRQPGVLATRVGYTGGDVANAT
                     YRNHGSHAEAIEILFDPERTSFRSLLEFFFQIHDPTTPNRQGNDRGPSYRSAIYHTNE
                     AQAHIAAATIAEVNASGRWPGKVVTEIEPAGPFWQAEPEHQDYLERFPNGYTCHFPRP
                     DWTLP"
     gene            complement(2614..4173)
                     /locus_tag="PLQ11_10945"
     CDS             complement(2614..4173)
                     /locus_tag="PLQ11_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRK25458.1"
                     /translation="MNIANLLVRSARVFPEAPALCHGKTLICTYRGLNQRALRIAGHL
                     AHGLGLAPGDRVGVFMKNCPEYLETLYGIWHAGLVAVPINAKLHVREALFALEDADAG
                     AAFVSADLAGDLAPLLAETGRRCAVLTPGTASFAAAAAAEPLPGPVERGPDDIAWLFY
                     TSGTTGRPKGVMLTNRNLLAMTMCYFSDVDPVDRQDSIVYAAPISHGAGMYNFAHVLA
                     AANHVVPESGGFNEAELVDLAAHYRRISLFAAPTMVKRLVDHVAATGADASGFKTIVY
                     GGGPMYVEDIRKGLAVMGDRFVQIYGQGESPMTITALSRFHLSDRSHPRYAERIASVG
                     VAQSLVEVRVSDAEGNPLPIGETGEVLARGDTVMAGYWRNPEATAKTLRDGWLFTGDM
                     GALDADGFLTLKDRSKDVIISGGTNIYPREVEEVLLTHPSVAEVSVIGRKHPEWGEEV
                     VAFVATIPGTAVPTAAELDALCLAAIARFKRPKAYRFVASLPKNNYGKVLKTELRDLD
                     AGLVVADAGKA"
BASE COUNT          761 a         1419 c         1352 g          755 t
ORIGIN      
        1 gagccagccc ggcacgcccc tgccctcgcc gaccgaactc gaccgccgct ttgcgaagaa
       61 tgcgccgatc tggtcgctca acggcggcgc ctccaacggc ttttccggca ggccgctgtt
      121 ctccgtccgg cgcggcacgc cggtggtgct ggcgatcacc aaccgcacgc attggtcgca
      181 ggtgctgacg gtgcgcggcc acgtcttccg cctgctgcat ccgctcgatg acggctggga
      241 gccctatttc ctcgacacgg tgcacctgcc cgacaattcg gtgtcgcgca tcgccttcga
      301 tgccgtcaat ccgggccgct gggcgatccg ctcgaccatc gccgaacatt acgacgccgg
      361 tgtctatacg tggttcgagg tggcgtgagg gcgaaagccg ctcatatccc cagcttgctt
      421 ttcagcagct cattgaccgc gccggggttg gccttgccgc ccgaagcctt catcacctga
      481 ccgacgaacc agccgagcag cgtcggcttt tctttggcct gcgccacctt gtcggggttg
      541 gcggcgatga tctcgtcgac gaccttttcg atggcgccgg tgtcggtgac ctgtttcaga
      601 ccgcgctttt cgacgatccc ggccggatca ccgccctcgg tatagacgat ctcgaacaca
      661 tccttggcga tcttgccgga gatggcaccc gaggcgatca gatcgacgat gccgccaagc
      721 tgggcggcgt tgaccgggct gtcggcaatg cccttgccgt ccttgttgag gcggccgaac
      781 aattcgttga tggtccagtt ggcgaccgcc ttgccgtcgc gccccttcgc cgcggcttca
      841 aagaaatcgg cgctttcgcg ctcggcgatc agcaccgagg catcataggc ggagaggcca
      901 tactggctga tgaagcgggc ctttttctcg tccggcaatt ccggcagcgc cgctttcagg
      961 ctgtcgacgt aatcctgcgg gaattccagc ggcaacagat ccggatcggg gaagtagcgg
     1021 taatcatgcg cctcttcctt ggagcgcatc gagcgggtct cacgcctgcc cggatcgtag
     1081 aggcgggttt cctgatcaat gctgccgcca tcctccagaa tgccgatctg acgccgcgcc
     1141 tcgacctcga tggcctggcc gatgaagcgg atcgaattga cgttcttgat ctcgcagcgc
     1201 gtgccgagct tgtcgcccgg cttgcgcacg gagacgttga cgtcggcgcg caggttgccc
     1261 ttctccatgt cgccgtcgca ggtgccgaga tagcgcagga tggtcctgag cttggacaca
     1321 taggcctgcg cctcttcggc cgatctgaga tcgggccgcg agacgatttc catcagcgcg
     1381 acgccggagc ggttcagatc aacaaaggaa ttgttcgggt cctgatcatg gatcgacttg
     1441 ccggcatcct gttcaagatg cagccgctcg atgccgacca cgatcggttc ggcctcgccc
     1501 ggcacatcga cgatcacctc accctcgccg acgatcgggt gcttgtactg gctgatctgg
     1561 tagccctgcg gcaggtcggg gtagaaatag ttcttgcggt caaagcgcga cttcaggttg
     1621 atctcggcct tgaggcccag cccggtgcgg atcgcctggg cgacgcattc ggcgttgatc
     1681 accggcagca tgcccggcat cgccgcatcg accaggctga catgggaatt gggctcgccg
     1741 ccgaatgcgg tcgaggcgcc ggagaacagc ttcgacttcg aggttacctg cgcatggatc
     1801 tccatgccga tcacgatttc ccagtcgccg gtggcgccct tgatcagctt cttcggattg
     1861 acagcaacgg acatgaaaaa cccttcggcc tcgcgcgcca cgcaggtggc ggttcatctg
     1921 cgctggttca gcgcaaacgg cgggcaaggt caaggggcgg aacgcagcag gcggtgctga
     1981 atggcgccgg caccaccctt gaccttgggc gcgcatgccc cacctttagc tcacggcgca
     2041 gggcgatcct gcgccggcgg ccgacaggaa ggaccatccg atgagtgaca agagcgaacg
     2101 ggcggtgctt gccggcggct gcttctgggg catgcaggac ctgttccgcc ggcagcctgg
     2161 cgtgcttgca acgcgggtcg gatataccgg cggcgatgtc gccaacgcca cctaccgcaa
     2221 ccacggcagc catgccgaag ccatcgagat cctgttcgat ccggagcgca ccagcttccg
     2281 cagcctgctg gaattcttct tccagattca cgaccccacc acgccgaacc ggcagggcaa
     2341 tgatcgcggc ccgagttacc gctcggcgat ctaccatacg aacgaggcac aggcgcacat
     2401 cgccgccgca accatcgccg aggtcaatgc ctccggccgc tggccgggca aggttgtcac
     2461 cgaaatcgag ccggcgggcc cgttctggca ggccgaaccg gagcatcaag actatctcga
     2521 acggtttccg aatggctata cctgccactt tccgcgcccg gactggacgc tgccgtaggg
     2581 gcaggccccg ctgtccggaa gcgcggcggc gcgttacgcc ttgcccgcgt ccgccacgac
     2641 aagacctgca tcgagatcgc gcaattcggt cttgagcacc ttgccgtagt tgttcttggg
     2701 caggctggcg acaaagcgat aggccttggg ccgtttgaac cgggcaatcg ccgcaaggca
     2761 cagggcgtcg agttcggcgg cggtcggcac cgcagtacct ggaatggtgg cgacaaaggc
     2821 caccacttcc tcaccccatt cgggatgctt gcgaccgatc accgacactt cggccacgga
     2881 aggatgggtc agcaggacct cctcgacctc gcgcgggtag atgttggtgc cgccggaaat
     2941 gatgacatcc ttcgagcggt ccttcagcgt caggaacccg tcggcatcaa gagcgcccat
     3001 atcgccggtg aacagccagc catcgcgaag tgtcttggcc gtggcctccg gattgcgcca
     3061 gtagccggcc atcaccgtat cgccgcgcgc cagcacctcg ccggtttccc cgatcggcag
     3121 cggattgccc tcggcatcgc tgacccgcac ctcgacaagg ctttgcgcga cgccgaccga
     3181 ggcaatccgc tcggcgtagc ggggatggct gcggtcactg aggtgaaacc gcgacagcgc
     3241 ggtgatggtc atcgggctct cgccctgacc gtagatctgg acgaaccgat cgcccatcac
     3301 ggcgagcccc ttgcggatat cctcgacata catcggcccg ccgccataaa cgatggtctt
     3361 gaagcccgag gcatcggcgc cggtggcggc gacatgatcg accagccgct tgaccatggt
     3421 cggcgcggca aacagcgaaa tccggcggta gtgggcggca agatcgacca gttcggcctc
     3481 gttgaaaccg ccggattcgg gcacgacatg gttggccgcc gccagaacat gggcaaaatt
     3541 gtacattccg gcgccgtgcg agatcggcgc ggcatagacg atgctgtcct gccggtcgac
     3601 cggatcgaca tcggagaaat agcacatcgt catcgccagc agattgcggt tggtcagcat
     3661 cacgcccttg ggcctgcctg tcgttcccga ggtgtagaac agccaggcga tatcgtccgg
     3721 accgcgctcc accggccccg gcagaggctc ggccgccgca gcagcggcaa aactggccgt
     3781 gccgggcgtc agaaccgcac agcgccggcc ggtttcggca aggagcgggg ccagatcacc
     3841 ggcgagatcc gcggagacaa aggccgcacc agcgtccgca tcctccagcg cgaacagtgc
     3901 ctcgcggacg tgcagcttgg cgttgatcgg cacagcgaca aggccggcat gccagatgcc
     3961 atagagcgtt tcgagatatt cggggcagtt cttcatgaac acacccaccc gatcgcccgg
     4021 cgccagaccg aggccatgcg ccagatggcc ggcgatgcgc aaggcgcgct ggttgagacc
     4081 gcgataggtg caaatgagcg tcttgccatg acaaagcgcc ggcgcctcgg gaaacacccg
     4141 ggcgctgcgc accagcagat tggcgatatt catccgtctg tctccagaaa gatccgcagc
     4201 cggcaccgcc gtcgggcaag gggccagccg ccctgatcag gcaaaaaggg cgccctcagg
     4261 ccgacttctt gcggccggcg cgcttgc
//
LOCUS       DAOMXG010000189         4217 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_11353_length_4217_cov_8.073763, whole genome shotgun sequence.
ACCESSION   DAOMXG010000189 DAOMXG010000000
VERSION     DAOMXG010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11353_length_4217_cov_8.073763"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..612
                     /locus_tag="PLQ11_10950"
     CDS             100..612
                     /locus_tag="PLQ11_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HRK25459.1"
                     /translation="MSLKDRTALARTVLAAARRQGLTIATAESCTGGMVAAALTDIAG
                     SSDVVDRGFVTYSNAAKTAMLGVPEGMIQAHGAVSAEVAAAMALGALKQSQAGLAVAV
                     TGIAGPAGATAGKPVGLVHFAVAARDGRVNRLEMRYGALGRRRVRAAATATALRLLAE
                     MLDAGAVQFA"
     gene            complement(600..2858)
                     /locus_tag="PLQ11_10955"
     CDS             complement(600..2858)
                     /locus_tag="PLQ11_10955"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HRK25460.1"
                     /translation="MASLEPGYGTVEVRVQSGTRLLLAAFVAAIFTSAFLLFAVQPMF
                     TRMVLPLLGGSPAVWSVAMVFFQTLLLAGYAYAHVLARYFSLKSGLALHLCLFALTAL
                     TLPIGIQAGTGEPPATGQELWLIGVFLVSVGLPFFAVSANGPLLQAWFARSSHPTAGD
                     PYYLYGASNFGSFAALLLYPTLVEPLLSLNGQAGTWSSGFVVLAMLIAAAGIAALRMP
                     GIAAAQKSEVAAPAPSWSARAGWVVLAMVPSGLLVAATAHLSTDVAASPFMWVIPLAL
                     YLATYMVVFRERLPFRMEIMERVVVMLGGALVLMIFTGEPFLFVSLALHLGFLTAATL
                     VCHARLYQARPAPSHLTTFYFCMSLGGVLGGLFCGLIAPNIFSRVAEYPILIVAALAC
                     LPSLRTLSVGNWQREVLPWVGAVVTALGIKLAVSQMPGSDLHSVIIGLIVLLIFAAMV
                     NFRRPLAAMVLVAGAFLMLIDVNLATRDILTMRSFFGVNKVQISNDGQFLRLTHGTTL
                     HGTMRLREADGSPAPQRPLPLSYYYEGSPMSQTVRAAQGRAPAGAEIGVVGLGSGSIA
                     CVGRPEDRFAFFEIDPVVVQIAREKSLFRFLSECAPNARMVIGDARLTLAKDTQLYDM
                     LLIDAFASDAIPVHLLTREAVDMYMRRIKPGGMLVMHISNRHLELSSVVAAIGQSLGL
                     AGAVRFDPANPEEFSKTSKAGSLVTALVKDADTLKPLVAGGWKPFEPSKTTVWTDDYA
                     NIVGAMWRAYAN"
     gene            complement(2957..4036)
                     /locus_tag="PLQ11_10960"
     CDS             complement(2957..4036)
                     /locus_tag="PLQ11_10960"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HRK25461.1"
                     /translation="MLTVLNHSESQHEEVDRFAAAPHPREMLSLIGHETAERELLDSF
                     RAGRLHHAWLIGGEEGIGKATLAYRMARFVLAHPDPGSMEVMLATDLSVPADHPVAAQ
                     IAAGAHPDLAVIRRAWDSQRKAVPTEIRVDDVRKGLDIFTKTAGSGGYRVAIVDACDD
                     LNASGANALLKTLEEPPARSLWLLIAHQPRRLLPTIRSRCRSLSLRPLEAETVARITR
                     GLPDFAEREPNLHMRAAALAEGSVRNALTLLDPKSLAFHDSLDSLMDNLPALDADAVD
                     RLAEQMAGRAGEASFRQFCDRVRHWLSGELHRRAPEPQALQPLAEVWERLNRQQIEAE
                     VYNLDRRPMVIGVMQGLSSLFPLRS"
     gene            complement(4030..>4217)
                     /locus_tag="PLQ11_10965"
     CDS             complement(4030..>4217)
                     /locus_tag="PLQ11_10965"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012318491.1"
                     /note="catalyzes the reversible phosphoryl transfer from
                     adenosine triphosphate (ATP) to thymidine monophosphate
                     (dTMP) to form thymidine diphosphate (dTDP); Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HRK25462.1"
                     /translation="QAADRFEQQDLDFHRTVRAAFLAIAAGEPQRCTVIDASQPAEAV
                     FAAVVAAVTSRLAAVPC"
BASE COUNT          845 a         1392 c         1366 g          614 t
ORIGIN      
        1 atcccgcacg gcccgagaaa cgcaactgat ccaatctggt cggaaagtgc tctaagtgaa
       61 ttggacctca ctgcaagtgt tctcaatgga gacgtcccga tgagcctgaa ggatcgcacc
      121 gccctggccc gcaccgtgct cgccgccgcc cgccggcagg gcctgaccat cgccacggca
      181 gaatcctgca ccggcggaat ggtcgccgca gcgctgaccg acattgccgg ctcgtcggat
      241 gtcgtggacc gcggctttgt cacctattcc aacgccgcca agacggcgat gcttggcgtg
      301 ccggaaggga tgatccaggc ccatggcgcg gtgtcggccg aggttgcggc cgccatggcg
      361 ctgggcgcac tcaagcaatc gcaagccgga ctcgctgtcg cggtcaccgg cattgccgga
      421 ccggcgggcg cgaccgcagg caagccggtg gggctggtgc attttgccgt tgcggcaagg
      481 gatgggcggg tcaaccggct tgaaatgcgc tacggagcac tcggccggcg acgggtgcgc
      541 gcggcggcca cagccaccgc gctccgtctg cttgccgaaa tgctcgacgc cggcgccgtt
      601 cagttcgcgt aggcacgcca catggcgccg acgatattgg cgtaatcgtc ggtccagacc
      661 gtggtcttgc tgggctcgaa cggcttccag ccgccggcaa caagcggctt gagcgtatcg
      721 gcatccttga ccagcgccgt gaccagcgac ccggccttgc ttgtcttgga gaattcctcg
      781 ggattggccg gatcaaaccg caccgcaccg gcaagcccca gcgactggcc gatcgcggcc
      841 acaacactgc tcagttccag atggcggttg gaaatatgca tgaccagcat gccgccgggc
      901 ttgatgcgcc gcatatacat gtccaccgct tcccgcgtca gcagatgcac cggaatggcg
      961 tcggaggcaa aagcgtcgat cagcagcatg tcataaagct gcgtatcctt ggccagcgtc
     1021 agccgcgcat cgccgatcac catccgcgca ttgggcgcac attcggacag gaaccggaac
     1081 agggactttt cccgggcgat ctgcaccacc accgggtcga tctcgaagaa ggcgaagcga
     1141 tcctcggggc gtccgacaca ggcgatcgat ccgctgccca gcccgaccac gccgatctcg
     1201 gcgcccgcag gggcacggcc ctgcgcggcc ctgaccgtct ggctcatcgg cgaaccctcg
     1261 tagtagtagg acagcggcag cggccgctgc ggggccggcg atccatccgc ctcgcgcagg
     1321 cgcatcgtgc catgcagggt cgtgccgtgc gtcagccgga gaaactgccc gtcgttgctg
     1381 atctgcacct tgttgacgcc gaagaacgag cgcatcgtca ggatgtcgcg cgtcgcaaga
     1441 ttgacgtcga tcagcatcag gaaggcgccg gcaaccagga ccatcgccgc aagcgggcgc
     1501 cggaaattca ccatggccgc gaagatcagc agaacaatca ggccaatgat gaccgaatgc
     1561 aggtcgctgc ccggcatctg cgagaccgcg agcttgatgc cgagtgcggt caccactgcg
     1621 ccgacccaag gcaggacttc acgctgccag ttgccgacac tcaatgtccg cagcgatggc
     1681 aggcaggcga gcgccgcaac gatcaggatc ggatattcgg cgacgcggga aaagatgttc
     1741 ggggcgatca gcccgcagaa caggccgccc agcaccccgc ccagcgacat gcagaagtag
     1801 aaggtggtca gatggctcgg cgccggacgg gcctgataga gacgggcatg gcagaccagc
     1861 gtcgcggccg tcaggaaccc aagatgcagc gccagcgaca cgaacaggaa gggttcgcca
     1921 gtgaagatca tcagcaccag cgcgccgccc agcatgacga caacgcgctc catgatctcc
     1981 atccggaacg gcagccgctc gcggaacacc accatgtacg tcgcaagata cagcgccagc
     2041 ggaatgaccc acatgaacgg cgaggccgcg acatcggtcg aaagatgcgc ggtcgcggcc
     2101 accagcaggc ccgaaggcac catcgccagc accacccagc ccgctctggc cgaccagctc
     2161 ggggctggtg ctgccacctc ggacttctgc gccgccgcga tgccaggcat gcgcagcgcg
     2221 gcaatccctg cggcggcgat gagcatcgcc agcacaacaa acccgctcga ccaggtgccc
     2281 gcctgcccgt tcagcgagag cagcggctca accagagtcg gataaagcag cagcgcggcg
     2341 aagctgccga agttcgaggc gccgtaaaga tagtagggat cgcctgcggt tggatgggaa
     2401 gagcgggcaa accatgcctg gagcagcggc ccgttggccg agacggcaaa gaacggcagc
     2461 ccgaccgaaa ccagaaacac gccgatcagc cacagttcct gcccggtcgc gggcggctca
     2521 ccggtgccgg cctggatgcc gatcggcagg gtcagcgctg tcagggcaaa caggcaaaga
     2581 tgcagggcca agccgctctt cagagaaaaa taccgcgcca gcacatgcgc ataggcatat
     2641 ccggcgagca gcagcgtctg gaagaacacc atcgctaccg accagaccgc cggggagcct
     2701 cccagcagcg gcaggaccat gcgggtaaac atcggctgga cggcaaagag caggaaggcc
     2761 gacgtgaaaa tggcggctac gaatgccgcc agcaatagcc gcgtgccgct ctgaacacga
     2821 acctcaactg ttccatagcc cggttccaag gatgccatct ctacccccaa tcctgtcgaa
     2881 tctaggggta tccgggtcgg cttaagacac ggttaagaat gattgcgcga cctgccgatc
     2941 ttgccggaat gcgtcgtcag gaccgtaggg ggaaaagact ggacaaaccc tgcatcacgc
     3001 cgatcaccat cggccggcgg tcgaggttgt agacctcggc ctcgatctgc tggcggttca
     3061 gcctttccca tacctcggcc agaggttgca gcgcctgagg ctccggcgcg cggcggtgca
     3121 gctcgccgct cagccaatgg cggacgcgat cgcagaactg gcgaaaactc gcttctccgg
     3181 cgcgaccggc catctgttcg gccagacgat ccaccgcatc ggcatcgagc gccggcaggt
     3241 tgtccatcag ggaatcgagc gaatcgtgaa acgccaggct cttgggatcg agcagggtca
     3301 gcgcgttgcg caccgatcct tctgccaggg ctgcggcgcg catgtgcaga ttgggctcac
     3361 gctcggcaaa gtccggcagg ccccgggtga tccgggcgac ggtctcggcc tcgagcggcc
     3421 gcagcgacag ggaacggcag cgcgaccgga tggtcggcag cagacggcgc ggctgatgag
     3481 cgatcagcag ccagagactg cgtgccggcg gctcttcaag cgtcttgagc agcgcattgg
     3541 cgccgcttgc gttgagatca tcacaggcat cgacgatggc aacgcgatag ccgcccgagc
     3601 cggcggtctt ggtgaagata tccagcccct tgcgcacatc atcgacacgg atttcggtcg
     3661 gcacggcctt gcgctggctg tcccaggccc ggcggatgac ggcaaggtca gggtgtgccc
     3721 cggcggcgat ctgcgccgcc accggatggt cggccggcac cgagagatcg gttgccagca
     3781 tcacctccat cgaacccggg tccggatggg cgagcacgaa gcgcgccatg cgataggcca
     3841 gcgtcgcctt gccgatgcct tcctcgccgc cgatcagcca ggcatgatgc aggcggccag
     3901 cccggaacga atcaagcaat tcgcgttcgg cggtctcatg gccgatcagc gacagcatct
     3961 cgcgcggatg cggcgcggca gcgaaacgat caacttcctc gtgctgactc tcgctgtgat
     4021 tgagaactgt cagcatggaa cagccgcgag ccgagaggtc acagcggcga ccacggcggc
     4081 aaaaaccgcc tccgccggct ggctggcgtc gatgaccgtg cagcgttgcg gctcgccggc
     4141 cgcaatcgcc agaaatgccg cccgcacggt ccggtgaaag tccagatcct gctgctcgaa
     4201 ccggtcggcc gcctgat
//
LOCUS       DAOMXG010000190         4206 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_11393_length_4206_cov_9.282101, whole genome shotgun sequence.
ACCESSION   DAOMXG010000190 DAOMXG010000000
VERSION     DAOMXG010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4206
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11393_length_4206_cov_9.282101"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1174
                     /gene="uxaC"
                     /locus_tag="PLQ11_10970"
     CDS             <1..1174
                     /gene="uxaC"
                     /locus_tag="PLQ11_10970"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008127463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HRK25463.1"
                     /translation="VPRADGGASEADARAIWRLFAQHWHLFRGTPTRLWFEQSLEDVF
                     GIDERLAPGNADRLFDRISEALARPECRPRALYERFGIKAISTTDSPIDDLGHHAALR
                     ASGWQGRVLPCYRPDPVVDPDFGGFRAAVARLGELTGEDTASWPGYLAAHRKRRAYFI
                     GMGATATDHGHPTARTENLDTAAAARLFQRVIAGEASAAEAEAFRGQMLTEMARMSLE
                     DGLVMQLHPGSFRNHSPDLYRRFGRDKGADIPTATNYVEALKPLLDAFGHDSRLTLIV
                     FTLDETAYSRELAPLAGVYPALRLGPAWWFFDAPEGMRRFRELTTETAGFYNTVGFND
                     DTRAYLSIPARHDMARRMDCAFLARLVAEHRLDEEEAHELAADLAWRLAEKAYKLD"
     gene            1252..2178
                     /locus_tag="PLQ11_10975"
     CDS             1252..2178
                     /locus_tag="PLQ11_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRK25464.1"
                     /translation="MAAASAQTTTLRSADTHPDGYPTVEAVKFMGQLIEQRTGGRYKI
                     QMFPSSTLGQEKDTIEQTRFGVIDMNRINMAPLNNLVPETQVPGLPFVFRSVEHMRKV
                     MDGPIGEEVLKAFEPHGMVGLAFYDSGARSFYNTKRPVTKLEDLKGLKIRVQQSDMFI
                     ALVNALGANATPMPFGEVFSALQTGVIDGAENNWPSYESTRHFEVAKHYSKTEHSMTP
                     EVLVMSKRSWDKLSDADKAVFRAAAKESVAKMRELWEAREKTAEAAVKAKGAQITEVD
                     KKPFIDAMKPVYDRFVTDAKLKDLLARIQAVQ"
     gene            2302..2838
                     /locus_tag="PLQ11_10980"
     CDS             2302..2838
                     /locus_tag="PLQ11_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRK25465.1"
                     /translation="MTALASLLARILAPISRACLWLAASGLVTMTLAVAWQVFGRKIL
                     NDTPTWTEPAALLLMSWFILFGAAVCLREGEHMGFDIGLHYAPPPLRFAMKAVTHVLI
                     IIFGLYMAVYGWQLTEKTWSAKMAGINIPQGMDYLPLFAGGILFALFAFENLVNQIAH
                     RRDTFVPVPGGHAPGGPE"
     gene            2844..4124
                     /locus_tag="PLQ11_10985"
     CDS             2844..4124
                     /locus_tag="PLQ11_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020051860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRK25466.1"
                     /translation="MELWVLFGSFTVLLLIGMPVAFCLGISSLMTVLWMGIPPIVVFQ
                     QINSGMNAFAMMAIPFFIFAGDLMIRGGIADRLINFAASLVGHLRGGLGQVNVLTCTL
                     FGGISGSAVADASAVGGIMIPQMKSRGYDADYAVNVTANAAIIALLIPPSHNMIIYSL
                     SAGGNLSIADLFTAGIVPGLMLCGALMLAAWAVAVRRGYPTGIFPGFRIVLRTLVVAV
                     PGILLIFIIFGGIRSGWFTATESSCIALVYAGLIAAFVYRGLGWKEFIEATHGAVRTT
                     AMVLLVIGAAGAFGWLMAFLQVPQTMVAWMKTISSDPFTILLLINIILLVLGTFMDMA
                     PMIIICTPIFLPVVTAFGIDPIHFGVILILNAGIGLNTPPVGSVQFVACAVGKISIGE
                     SMKTIWPFYGVSVLVLLLVTYVPEFSLWLPRLFK"
BASE COUNT          680 a         1350 c         1324 g          852 t
ORIGIN      
        1 ggtgccgcgc gccgatggcg gcgcctcgga ggccgatgcg cgggccatct ggcggttgtt
       61 cgcgcagcac tggcacctgt ttcgcggcac gccgacccgg ctgtggttcg aacaatcgct
      121 tgaggatgtg ttcggcatcg acgagcggct ggctccgggc aatgccgacc ggctgttcga
      181 ccggatttcc gaggcgctgg cccgccccga atgccgcccc cgcgcactct acgagcgctt
      241 cggcatcaag gcgatttcga caaccgacag cccgatcgac gatcttggcc atcacgccgc
      301 gctgcgcgcc tccggctggc agggcagggt gcttccctgc tatcggccgg acccggtggt
      361 cgatcccgac ttcggcgggt ttcgcgcagc cgtggcgcga ctgggtgaat tgaccggcga
      421 ggatacggca agctggccgg gttatcttgc cgcgcatcgc aagcgccgcg cctatttcat
      481 cggcatgggc gccacggcga ccgaccacgg gcatccgaca gccaggaccg agaacctcga
      541 caccgccgca gcggcgcggc ttttccagcg tgtcatcgcc ggtgaggcga gcgcggcgga
      601 ggcagaggcg ttccgcggcc agatgctcac cgaaatggcc cggatgagtc tggaagacgg
      661 gctggtcatg cagctccatc ccggatcgtt ccgcaatcac agcccggatc tctaccggcg
      721 cttcggtcga gacaagggcg ccgacattcc gacggcgacc aactatgtcg aggcgctcaa
      781 accgctgttg gatgctttcg gccacgacag ccggctgacg ctgatcgtgt tcacgctgga
      841 tgagactgcc tacagccggg agctggcgcc gctcgccggc gtctatccgg cgctgcggct
      901 cggcccggcc tggtggtttt tcgatgcgcc cgaaggcatg cggcgctttc gcgaactgac
      961 caccgagacg gcgggcttct acaacacggt cggcttcaac gacgacaccc gtgcctatct
     1021 gtcgatcccg gcccgccacg acatggcccg gcggatggat tgcgcctttc ttgcccgcct
     1081 cgtcgccgaa caccggctgg atgaggaaga agcccatgaa ctggcggccg accttgcctg
     1141 gcggcttgcc gaaaaagcct acaaactgga ctgataacgg aggaacctgc catgaccagc
     1201 cgacgcaatg ccttgaaatc gaccctggcc gccgccgctc ttgccttcgg catggcggcc
     1261 gccagcgcgc agaccacaac gctgcgctcc gctgataccc atcccgatgg ctatccgacg
     1321 gtggaagcgg tcaagttcat gggccagctg atcgagcagc gcaccggcgg ccgctacaag
     1381 atccagatgt tcccgtcctc gacgctcggc caggagaagg acacgatcga gcagacccgc
     1441 tttggcgtca tcgacatgaa ccgcatcaac atggcgccgc tgaacaatct ggtgccggaa
     1501 acgcaggtgc cggggctgcc cttcgtgttc cgctcggtcg agcacatgcg caaggtcatg
     1561 gacgggccga tcggtgagga ggtgctcaaa gccttcgagc cgcatggcat ggtcgggctc
     1621 gccttctacg attccggcgc gcgctccttc tacaacacca agcgcccggt gaccaagctg
     1681 gaggacctca agggcctcaa aatccgcgtg cagcagtccg acatgttcat cgcgctggtc
     1741 aatgcgcttg gcgccaacgc cacgccgatg ccgttcggcg aagtgttctc ggcgttgcag
     1801 acgggggtca tcgacggggc cgagaacaac tggccatcct atgaatcgac acggcatttc
     1861 gaggtcgcca agcattactc caagaccgaa cactcgatga ctccggaagt gctggtgatg
     1921 tccaagcgct cctgggacaa gctctcggat gcggacaagg cggttttccg tgctgctgcc
     1981 aaggaaagcg tcgccaagat gcgtgaactc tgggaagccc gcgaaaagac cgccgaggca
     2041 gccgtcaagg ccaagggcgc gcagatcacc gaggtcgaca agaagccgtt catcgacgcg
     2101 atgaagccgg tctacgaccg tttcgtcacc gatgcgaagc tgaaggacct gctcgcccgc
     2161 attcaggcgg tgcagtgatc ctcctgcctc tttccgcctg gctcacgctc acgttttgat
     2221 ggactcatca aggcgggagc gtgatcgtta tctgtgtcct gatgcgcctg tcaggacaca
     2281 gcgtgatcca gggagccgcg catgacagcc cttgcttcgc ttctcgcccg cattcttgcg
     2341 cccatctcgc gcgcctgcct ctggctcgcg gcgagcggac tcgtcaccat gaccctggct
     2401 gttgcctggc aggtattcgg ccgcaagatc ctcaacgaca ccccgacctg gaccgagccg
     2461 gcggcgctgc tgctgatgag ctggttcatc ctgttcggcg cggcggtctg cctgcgcgag
     2521 ggcgagcaca tggggtttga catcggcctc cactacgcgc cgccgccctt acgctttgcc
     2581 atgaaggcgg tcacccatgt gctgatcatc atcttcggcc tctacatggc ggtttatggc
     2641 tggcagctca ctgaaaagac ctggagcgcc aagatggcgg ggatcaacat cccgcagggg
     2701 atggactatc tgccgctgtt tgccggcggc atcctgtttg cgctgtttgc cttcgagaat
     2761 ctggtcaacc agatcgcgca tcgtcgcgat acattcgttc ccgtccccgg cggtcacgcg
     2821 ccgggcggcc cggagtgatc cccatggaac tctgggttct gttcggctcg ttcaccgttc
     2881 tgctgctgat cggcatgccg gtggcgttct gcctcggcat ctcctcgctg atgacggtgc
     2941 tgtggatggg cattcccccc atcgtggtgt tccagcagat caattccggc atgaacgcct
     3001 ttgccatgat ggcgatcccg ttcttcatat tcgccggcga tctgatgatc cgtggcggca
     3061 ttgccgaccg gctgatcaac tttgccgcca gtctcgtcgg ccatctgcgc ggcgggctcg
     3121 gtcaggtcaa tgtgctgacc tgcacgctgt tcggcggcat ttccggttcg gccgtggccg
     3181 atgcgtcggc ggttggcggc atcatgatcc cgcagatgaa gagccgcggc tatgacgccg
     3241 attatgcggt gaatgtcacg gcgaatgcgg cgatcatcgc gctgctgatt ccgccgtcgc
     3301 acaacatgat catctattcg ctgtcggccg gcggcaatct ttcgattgcg gatctgttca
     3361 cggccggcat tgtgccgggt ctgatgctgt gcggggcgct gatgctggcg gcctgggcgg
     3421 ttgcggtcag gcgcggctat ccgaccggca tatttcccgg ctttcgcatc gtgctgcgca
     3481 cgctggtcgt cgccgttccc ggcattctgc tgatcttcat cattttcggc ggcatccgtt
     3541 ccggctggtt caccgccacc gaaagctcgt gtatcgcgct ggtctatgcg ggcctgatcg
     3601 cggccttcgt ctatcgcggc cttggctgga aggaatttat cgaggcgacg catggagcgg
     3661 tccgcaccac ggcgatggtg ctgctggtta tcggggcggc gggggcgttc ggctggctga
     3721 tggcctttct ccaggtgccg cagacgatgg tggcctggat gaagacgatc tcgagcgatc
     3781 ccttcaccat cctgttgctg atcaacatca tcctgctggt gctcggcacc ttcatggaca
     3841 tggcgccgat gatcatcatc tgcacgccga tctttctgcc ggtggtgacc gccttcggta
     3901 tcgatcccat ccatttcggc gtgatcctga tcctcaatgc ggggatcggg ctcaacacgc
     3961 cgccggttgg ctcggtgcag ttcgtggcct gcgctgtcgg caagatctcg atcggcgaga
     4021 gcatgaaaac catctggccg ttttatggcg tatcggtcct ggtgctgctg ctggtgacct
     4081 atgttccgga attctcgctg tggctgccga ggctgttcaa gtgatgcgat tgagcctttc
     4141 cacccttgcg tccttgcccg gctcggttgc ggttcccaaa ggccgctggt caggtcccgg
     4201 catcgt
//
LOCUS       DAOMXG010000191         4202 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_11407_length_4202_cov_13.825175, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000191 DAOMXG010000000
VERSION     DAOMXG010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11407_length_4202_cov_13.825175"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="PLQ11_10990"
     CDS             complement(<1..448)
                     /locus_tag="PLQ11_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018045162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRK25467.1"
                     /translation="MTLATGASVPPGQPAVRLDQVHLSLGAGASRVHILKGLSLTVAA
                     GEAVGLVGPSGSGKSTLLMVAAGLERPDSGEVEIVGQNLSALNEDALARFRGARIGIV
                     FQAFHLIPTMTALENVAIPLELAGRSDAFDRAREELIAVGLGERLGH"
     gene            530..1195
                     /locus_tag="PLQ11_10995"
     CDS             530..1195
                     /locus_tag="PLQ11_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HRK25468.1"
                     /translation="MIRNTPAPLASPTRRSLLAAASLVAGSRLASGAAPVRIVALGDS
                     LTAGYLLPADAAFPAVLEKALAQRGLAVTITNAGVSGDTSAGGLARLDWSVAEGTAGV
                     VVELGANDALRGLDPVRTEANLDAILSRLAKRNIRVLLAGMLAPRNNGPDYVAAFDGL
                     YPRLAAKHGVPLYPFFLDGVTGVAGMTLPDGLHPSREGVAEIVRRILPMAEQFVRDLT
                     AAK"
     gene            1277..1846
                     /locus_tag="PLQ11_11000"
     CDS             1277..1846
                     /locus_tag="PLQ11_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25469.1"
                     /translation="MLDLWKDPWPLDVKQCPCDVHFTDWMKREGRSDATVFHFGTGDH
                     HHVGKTLAMAGQAASVLGITATKPEYVSYMDLVIAHPQIGKRYKVLFTDIYQLAPELL
                     PKFDVVTLFHLCEFWSQANAPYARLDDAGVLQAMAGKVRAGGALLFYTGSFAFETARK
                     LVEAEIGRLGFDGPERFESLLVYRKAKAA"
     gene            2052..2615
                     /gene="thpR"
                     /locus_tag="PLQ11_11005"
     CDS             2052..2615
                     /gene="thpR"
                     /locus_tag="PLQ11_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HRK25470.1"
                     /translation="MPRLFTGIELPQEIAGRLAELRGGLPGARWLDPSDYHITLRFAG
                     DIDRRRAEDFAAELADIRFQPMVIALTGLASFGGGKPHSVHVSVAPNPALIELSEAHE
                     RAARRAGLAAEKRKFVPHVTLARLRGASTLDVADYLASRGGFPPLRFMVQRFVLFSAR
                     EQTGGGPYIIEAAFEPDRRGPILGSLA"
     gene            complement(2758..>4202)
                     /locus_tag="PLQ11_11010"
     CDS             complement(2758..>4202)
                     /locus_tag="PLQ11_11010"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRK25471.1"
                     /translation="FDQISRIQPLPPGLLPNIRAFYASRDGAPLWLEGNGLSLRAQRL
                     VALIERAEEHGLDGRRFAPLLAAARSSGDAMLREIAVSVAAAHYARDARGGRINPMQV
                     SRLITASPPLAEPIGVLEALRQAAEPALVLEAYHPRHSGYHALKAKLAELRKTAPVAQ
                     PAPVPSGPVLRPGMADSRVPLLRQRLGMPESADPIYDADLTMAVKAFQARHGLKASGT
                     LTPRTLAALNGGNEPASPVGDIIANMERWRWLPVDLGNDHVFVNVPSFALTLQQNGER
                     VLQTRVIVGKPETQTPIFSDRIQFLVVNPYWNVPPSIALKEMLPLLQQNPYALQARGL
                     EVVSRGRVVDPSTVDWTQAGRSVAIRQPPGERNALGHIKIMFPNQHAVYLHDTPSRGL
                     FQRATRAFSHGCVRVENPFRLAGALLSEAGSEGRLRAMVGGAERQVRLPAEVPVHLAY
                     FTTFVDEAGGLVHFPDLYGHNRRLKALLGI"
BASE COUNT          763 a         1289 c         1389 g          761 t
ORIGIN      
        1 agtggccgag ccgctcgccg aggccgacgg cgatcaactc ctcgcgcgcc cggtcgaaag
       61 catcgctgcg gccggcgagt tccagcggaa tggcgacatt ctccagcgcc gtcatggtcg
      121 ggatgagatg gaaggcctga aagacaatgc cgatccgtgc gccgcgaaag cgggcgagcg
      181 catcctcatt cagcgccgac aggttctggc cgacgatctc gacctcgccc gaatccgggc
      241 gctccagccc ggcggccacc atcagcaatg tcgacttccc cgaaccagag gggccaacca
      301 ggcccaccgc ttcgccagcg gcgaccgtca gcgacagccc cttgaggata tgcacacgcg
      361 atgcgccggc gccgaggctg agatgcacct gatcgaggcg gacagccggc tggccgggcg
      421 gcaccgaggc gccggttgcg agggtcatga aggctccgtg gccaagaaaa tcctgcgaag
      481 aggcgaatgc tctctggcag ataggcgctg gaaggcttaa ctccaaggga tgatccgcaa
      541 tacgccagcg cccctcgcaa gcccaacccg ccgctcgctg cttgcggctg cctcgctggt
      601 ggccggttca cgccttgcat cgggcgcggc tccggtccgc atcgttgcct tgggcgatag
      661 tctgaccgcc ggttacctgt tgccggccga tgcggccttt ccggccgtgc tggagaaggc
      721 gctggcgcag cgcggtcttg cggtgaccat caccaatgcc ggcgtgtccg gcgataccag
      781 cgcgggcggg ttggcaagac tcgactggtc ggtggcggag ggaaccgccg gtgtggtcgt
      841 cgaactcggc gccaacgatg cgttgcgcgg cctcgacccc gtacgcaccg aggccaatct
      901 cgatgccatt ctctcccgtc tggcaaagcg caacatccgc gtgctgctgg ccggcatgct
      961 ggcgccgcgc aacaacggac ccgattatgt cgccgccttc gacggtctct atccgcgcct
     1021 ggcggcaaag cacggggtgc cgctctatcc gttctttctc gacggggtca ccggggttgc
     1081 cggcatgacc ctgcccgatg ggctgcatcc ctcgcgtgag ggcgttgcgg aaatcgtcag
     1141 gcgtatcctg cccatggccg aacagttcgt ccgggatctg accgccgcaa aatgaggggt
     1201 ccgcagcctt gccttgccgg atttcttgac cggagccggc attcgcttca cttggacgtg
     1261 cattgaggag gatgacatgc tcgacctttg gaaagatccc tggccgctcg atgtgaagca
     1321 gtgcccgtgc gatgtgcatt tcaccgactg gatgaagcgc gaggggcgct cggatgctac
     1381 ggtctttcat ttcggcaccg gcgatcatca ccatgtcggc aagacactgg cgatggcagg
     1441 gcaggctgcc tcggtgcttg ggatcacggc gaccaagcca gagtatgtca gctacatgga
     1501 tctggtgatc gcccatccgc agatcggcaa gcgctacaaa gtgctgttca cagacatcta
     1561 ccagctcgcc cctgaactct tgccgaagtt cgatgtggtg acgctgtttc atctctgcga
     1621 attctggagc caggcgaatg cgccctatgc gcggcttgat gatgccggtg tgctccaggc
     1681 catggccggc aaggttcggg ccggaggagc actgctgttc tacaccggct cctttgcttt
     1741 cgagacggcc cgcaaactgg tcgaggccga aatcggccgg cttggctttg acgggccgga
     1801 gcggttcgaa tcgctgctcg tctatcgcaa ggccaaggcg gcctgagcgg tctttgacgg
     1861 tgctagagca ttttcgagcg aagtggtgtc cggttcgcgt gaagaaaatg cgatagaaca
     1921 agaaattaga gcattttcga gcgaagtggt gtccggttcg cgtgaagaaa atgcgataga
     1981 acaagaaatt ggagcatttt cattgcatgt gaatgcacgg aaaattctcc ggacttgttg
     2041 cggagggttg catgccccgg ctgttcacag gaatcgaact gccgcaagag attgccggac
     2101 gccttgcaga actgcgcggc gggctgccgg gcgcgcgctg gctggatccg tccgactatc
     2161 acatcacctt gcggtttgcc ggtgatatcg accgccggag ggccgaagac tttgccgcag
     2221 aactggccga tatccgtttc cagccgatgg tcatcgcgct gaccgggctc gccagtttcg
     2281 gcggaggcaa gccgcattcg gtgcatgtga gcgttgcgcc aaacccggcg ctgatcgaac
     2341 tcagcgaagc gcatgaacgg gcggcgcggc gcgccgggct tgccgctgaa aagcgcaagt
     2401 tcgtgccgca tgtcacactg gcacgcctgc gtggtgcatc aacgcttgat gtcgccgatt
     2461 acctcgcctc gcgcggcggt tttccgcccc tgcggttcat ggtccagcgt tttgtgctgt
     2521 tctcggcgcg cgagcagacc ggcggcgggc cctatatcat cgaggctgcc ttcgagccgg
     2581 atcggcgagg ccccatcctg ggcagccttg cctaaggcat gctgcaaaaa ggggcaacgg
     2641 cctttagata acgccgcatt ttgatggaat catcaaaatg cggataacgt gatcggtatc
     2701 aataagttag agcatgtctt ctgcgattga ccggtatcca cttaatcgcg acatgctcta
     2761 gatgcccagc agcgccttga gccggcggtt gtggccataa agatcgggga agtgaaccaa
     2821 cccgccggcc tcgtccacga aggtggtgaa gtaagcaaga tgcaccggca cctcggcggg
     2881 cagccgtacc tgccgctcgg ctccgccgac catggcccgc aggcggcctt cgcttcctgc
     2941 ttcactcagc aaggcgccgg caaggcggaa ggggttctcg acgcgcacgc agccatggct
     3001 gaaggccctg gttgcacgct ggaacaggcc gcggcttggc gtatcgtgca ggtagaccgc
     3061 gtgctggttg ggaaacatga tcttgatgtg ccccagggcg ttgcgttctc ccggcggctg
     3121 tcgaatggcg acagaacggc cggcctgggt ccaatccaca gttgacggat cgacgacgcg
     3181 gccacggctg accacctcaa gcccgcgtgc ctgcagcgca taggggttct gctggagcaa
     3241 cggcagcatt tccttgagtg cgatcgaggg ggggacattc cagtaggggt tgaccaccag
     3301 gaactggatg cgatcggaga agatcggcgt ctgcgtctcc ggtttgccga caatgacgcg
     3361 ggtctgcaag acacgttcgc cgttctgctg caaggtcagc gcaaaactcg gcacgttgac
     3421 gaaaacgtgg tcattgccaa gatcgaccgg cagccagcgc cagcgttcca tgttggcgat
     3481 gatgtcgccg acagggctgg ccggttcatt gccgccgttg agggcggcaa gcgtgcgcgg
     3541 cgtcagcgtt ccgctggcct tgaggccatg gcgggcctga aacgccttca cggccatcgt
     3601 cagatcggca tcatagatcg gatcggcgct ttccggcata ccgagacgct ggcgcagcaa
     3661 tggcacgcgg ctgtcggcca ttccgggacg caggacaggg ccggacggca caggcgccgg
     3721 ctgggcaacc ggtgcggtct tgcgcagttc cgccaatttg gccttcagcg cgtgatagcc
     3781 ggagtggcgc gggtgatagg cctcaagcac cagagccggt tcagcggcct gacgcagggc
     3841 ttcgaggacg ccaatcggct cggccagggg tggcgaggcg gtgatgagcc gcgagacctg
     3901 catgggattg atccggccgc cccgcgcgtc gcgggcataa tgggcggctg cgaccgagac
     3961 ggcgatctca cgcagcatcg catccccgga actgcgcgcc gcagcaagca gcggtgcgaa
     4021 gcgacggccg tccagcccat gttcctcggc acgctcgatc aatgcgacca ggcgctgggc
     4081 gcgcagcgac aacccattgc cctccagcca cagcggcgca ccgtcgcggc tggcatagaa
     4141 ggcccggata ttgggcagca ggccgggtgg cagaggctga atgcggctta tctgatcgaa
     4201 ca
//
LOCUS       DAOMXG010000192         4200 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_11420_length_4200_cov_10.447045, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000192 DAOMXG010000000
VERSION     DAOMXG010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11420_length_4200_cov_10.447045"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="PLQ11_11015"
     CDS             <1..420
                     /locus_tag="PLQ11_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018409762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HRK25472.1"
                     /translation="AIVGLIDAGAPQIRITNRTRSSAEALAAEFGNRLSVVDWSERDM
                     ATAGVGLMVNTTSLGMVWQPPLDIRLDDLPREAVVNDIVYVPLETGLLRAARQRGNRV
                     VDGLGMLLHQARPAFRAWWGILPEVTPGLRQRIEETL"
     gene            417..1646
                     /locus_tag="PLQ11_11020"
     CDS             417..1646
                     /locus_tag="PLQ11_11020"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HRK25473.1"
                     /translation="MMLPVTVEMVRQAEAVLKGAVARTDCNKAQTLGEIVGAELWLKF
                     ENHQFTATFKERGALNRLNALTADQCRRGVIAMSAGNHAQGVAYHARRLGIPATIVMP
                     EGTPIVKIENTRRHGAEVIVTGATLEDSARFAHEHGAQNGLTFIHPYDDPLVIAGQGT
                     LGLEMLASAPHLDTLVIPIGGGGLISGIAVAARALRPDIRIIGVQSALYPSMYNVIKG
                     ESLPMRGDTLAEGIAVKSPGRITTELVRALVDDILLVSEPDIEQALALLINIEKTVVE
                     GAGAAGLAAVLAYPEAFRGRAVGLVLCGGNIDTRLIASVLTRELAREGRLSQLTIDLV
                     DRPGQLARIATLLGEAGANIVEVAHQRVFSDLPAKGTLLDVVIETRDRAHRDAAVAHL
                     RGAGFKVDLHTNPGGTH"
     gene            1874..3049
                     /locus_tag="PLQ11_11025"
     CDS             1874..3049
                     /locus_tag="PLQ11_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRK25474.1"
                     /translation="MTKLTRKLACAAGLLALMASAAAAQEPLKIGIVSVLSGPQATLG
                     QQLRDGFQLAVKDMGGKLGGRPVELVIVDDELKPDVAVTKVRTVLERDKVDFVVGPIF
                     SNILQAIHKPVVDSGTILISPNAGTSNFAGKQCHENFFVTSYQNDQPHEVLGKYAQDK
                     GFKRAYIMAPNYQAGKDSLAGFKRHFKGEIVDEVYTQLGQLDFAAELAKIAAAKPDVF
                     FVFMPGGMGVNLVKQFRQAGLADKVPFLSAFTVDESTLPAQQDAALGFLSGMSWAPNM
                     DNPQSKRFVAGYEAAYSIVPGSYAMQAYDAAQLIDSALKQTKGSTADKAALRAALKKA
                     DFTSLRGKFKFNTNGYPIQDFYVVKVAKRADGKFQTEILEKVFSDYGDAYAKDCELK"
     gene            3277..4200
                     /locus_tag="PLQ11_11030"
     CDS             3277..4200
                     /locus_tag="PLQ11_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009209335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRK25475.1"
                     /translation="MLSLFIIQVLNGLQFGILLFLVAAGLTLVFGVMDFINLAHGVQY
                     MLGAYLAVMFIGMTGSFPLGLVLALAVALAIGLALEFLVFRHLYARSHLDQVLATFGL
                     ILLVNQSVKLLWGAAPLSVPVPEWLSGSVMLGDGILYPVYRFAILFAGLAVAGLLWFI
                     VEKTRTGMLVRAGASNAPMVSALGVDINKLFMVVFAFGTMLAGFAGAMAAPILSVEPS
                     MGDSVLILAFVVIVIGGIGSIRGAFFAALLVGLVDTLGRSYSVDFLRLFTGPAAARGI
                     AGGLSSMFIYLLMAVVLFFRPNGLWPARGRA"
BASE COUNT          703 a         1324 c         1375 g          798 t
ORIGIN      
        1 gccatcgtcg gcctcatcga tgccggggcg ccgcagatcc gcatcaccaa ccgcacccgc
       61 agcagcgccg aggccctggc cgccgaattc ggcaaccggc tcagcgtcgt ggactggagc
      121 gaacgcgata tggccacggc aggcgtcggg ctgatggtca ataccaccag cctcggcatg
      181 gtctggcagc cgccgctcga cataaggctc gacgatctgc cgcgcgaggc cgtggtcaac
      241 gatatcgtct atgtgccgct ggagacaggt ctgctgcgcg cggcccggca gcgcggcaac
      301 cgtgtggtgg acgggctcgg catgctgctg catcaggcgc gcccggcctt ccgggcctgg
      361 tggggcatcc tgccggaggt cacgcccggt ttgcgccaga ggatcgagga gacgctatga
      421 tgcttcctgt gacagtcgag atggtgcggc aggccgaggc cgtgctgaag ggcgcggtcg
      481 cccggaccga ttgcaacaag gcgcagacgc tcggcgaaat cgtcggcgcc gaattgtggc
      541 tgaagttcga gaaccaccag ttcaccgcaa ccttcaagga gcgcggcgcg ctcaaccggc
      601 tcaacgccct gacagccgat caatgccggc gcggcgtcat cgcgatgtcc gccggaaacc
      661 atgcccaggg ggttgcctat cacgcgcgcc ggctcggcat tccggcgacg atcgtgatgc
      721 ccgaaggcac gccgatcgtg aagatcgaga atacccgccg ccacggcgcc gaagtgatcg
      781 tcacaggcgc tacgctggag gattccgccc gctttgccca tgagcatggc gcacagaacg
      841 ggctgacctt catccaccct tatgacgatc cgctggtgat cgccggccag ggcacgctgg
      901 ggctggagat gctggccagc gctccgcatc tcgatacgct ggtcattccc atcggcggcg
      961 gcgggctgat ctcgggcatc gctgtggcag cgcgcgccct gcgcccggac atccgcatca
     1021 tcggggtgca gtcggcgctc tatccgtcga tgtacaacgt catcaagggc gaatcgctgc
     1081 cgatgcgcgg cgatacgctg gccgaaggca ttgccgtcaa atcgcccggg cgcatcacca
     1141 ccgaactcgt gcgcgcgctg gtcgatgaca tcctgctggt cagcgaaccg gatatcgaac
     1201 aggcgctggc cctgctgatc aacatcgaaa agaccgtggt ggaaggcgcg ggcgcagccg
     1261 ggctggcggc ggttctggcc tatcccgaag cgttccgcgg tcgcgccgtc gggctggtcc
     1321 tgtgcggcgg caacatcgat acacggctga tcgccagcgt tctgacccgg gaactggcgc
     1381 gcgaaggccg gctcagccag ctgacgatcg atcttgtcga ccggcccggt cagctcgccc
     1441 gcatcgcgac cctgctgggc gaggcagggg ccaatatcgt cgaagtggcc caccagcggg
     1501 tgttttccga tcttccggcc aaagggaccc tgctcgatgt ggtcatcgaa acgcgcgacc
     1561 gggcgcatcg ggatgccgct gtcgcccatc tgaggggcgc tggcttcaaa gtcgatcttc
     1621 ataccaatcc tggcggcacg cactgaaaca accctcctcc ggcaaggcgc ggcgtcgccg
     1681 acgccacccg gcgcgggatt gacagggccg ggaccgctgc tagattgttt aaacttaaaa
     1741 tatctgccgg gcgcaggccg cgcattggtg gacgggagga tgaggcgatc aggaatgcac
     1801 agcagcgctt gagaggcgcg gagcatacgt gccatagtcg ccgcagcgtt caaggatgat
     1861 caggagggga tcaatgacca aactcactcg taagctggct tgtgccgccg gcctgctggc
     1921 cctgatggct tcggccgcag ccgcacagga gccgcttaag atcggtatcg tcagcgtgtt
     1981 gtccggcccg caggcgacgc ttggccagca gctgcgcgac gggttccagc tcgccgtcaa
     2041 ggacatgggc ggcaaactcg gcggccgtcc tgtcgaactg gtcatcgtcg atgacgaact
     2101 caagcccgac gttgcggtga ccaaggtccg caccgtgctg gagcgcgaca aggtggactt
     2161 cgtcgtcggt ccgatctttt ccaacatcct gcaggctatc cacaagccgg tggtcgattc
     2221 cggcacgatc ctgatctcgc cgaacgccgg aacctcgaat ttcgccggca agcagtgcca
     2281 cgagaacttc ttcgtcactt cctaccagaa cgaccagccg cacgaggtgc tgggcaagta
     2341 tgcgcaggac aagggcttca agcgcgccta catcatggcg cccaattatc aggccggaaa
     2401 ggactcgctg gccggcttca agcgccattt caagggcgag atcgtcgatg aggtctatac
     2461 ccagctcggc cagctcgatt tcgcggcgga actcgccaag atcgctgctg ccaagccgga
     2521 tgtgttcttc gtgttcatgc cgggcggcat gggtgtcaat ctggtcaagc agttccggca
     2581 ggccgggctt gccgacaagg tgccgttcct ctcggctttc acggtggatg aatccaccct
     2641 tccggcgcag caggatgccg ccctcggctt cctctccggc atgagctggg cgcccaacat
     2701 ggacaatccg cagtcgaaga ggttcgtggc gggctatgaa gctgcttaca gtatcgtgcc
     2761 cggctcctat gccatgcagg cctatgacgc ggcgcaactg atcgactcgg cgctgaagca
     2821 gaccaagggg tccaccgccg acaaggcggc cctgcgcgcg gcgctgaaga aggcggattt
     2881 cacctcgctg cgcggcaagt tcaagttcaa caccaatggc tatccgatcc aggacttcta
     2941 tgtggtgaag gtggccaagc gcgccgatgg caagttccag accgagatcc tggaaaaggt
     3001 gttctccgat tacggcgatg cctatgccaa ggattgcgag ctgaagtgag cgggcgccgg
     3061 tcagtccctc cccgttcggg gggagggtct cgcgatgtcc ccgccctacg ctcaccgtca
     3121 ttgcgagcgc gagcgaagca atccagctcc tgatcaagag actggattgc cacgtcgggc
     3181 cacagccctc ctcgcaatga cggcaagatc atgccgcctc gcccatcttc cctcccaggt
     3241 attacggatg agagatttga cggactttgt gccctcatgc tgagcctgtt catcattcag
     3301 gtcctcaacg ggctgcaatt cggcatcctg ctgtttctgg tcgctgccgg cctgacgctg
     3361 gttttcggcg tgatggattt catcaatctc gcccatggcg tgcagtatat gctcggcgcc
     3421 tatctggcgg tgatgttcat cggcatgacc ggatcatttc cgctcgggct ggtgctggcg
     3481 ctcgccgtgg cgctggcaat cgggctggcg ctggaatttc tggtcttccg ccatctctac
     3541 gcccgcagcc atcttgatca ggtgctggcc accttcgggc tgatcctgct ggtcaaccag
     3601 tcggtgaagc tgctgtgggg ggctgcgccg ctgtcggttc ccgtgccgga atggctgtcg
     3661 ggctcggtga tgctgggcga tggcatcctc tatccggtct accgctttgc catcctgttc
     3721 gccgggctgg cggtggccgg gctcttatgg ttcatcgtcg agaaaacccg caccggcatg
     3781 ctggtcaggg cgggcgcctc caatgccccg atggtctcgg cgctcggggt cgacatcaac
     3841 aagctgttca tggtggtgtt tgccttcggc accatgctgg cggggtttgc cggggcgatg
     3901 gcggcgccga tcctttcggt cgagccgagc atgggcgatt cggtgctgat cctggccttc
     3961 gtggtgatcg tgatcggcgg aatcggctcg atccgcggcg cgttctttgc ggccctgctg
     4021 gtcgggctgg tggatacgct gggccggtcg tattcggtcg attttctcag gctgttcacc
     4081 gggccggcgg cggcgcgcgg catcgccggc ggattgtcgt cgatgttcat ctacctgttg
     4141 atggctgtcg tcctgttctt ccggcccaac gggctgtggc cggcgcgggg gcgggcatga
//
LOCUS       DAOMXG010000193         4147 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_11619_length_4147_cov_10.597996, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000193 DAOMXG010000000
VERSION     DAOMXG010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11619_length_4147_cov_10.597996"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="PLQ11_11035"
     CDS             complement(<1..272)
                     /locus_tag="PLQ11_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HRK25476.1"
                     /translation="MSHSSFPVPMTAGRSASLRGRVRVPGDKSISHRALIFGLLAVGE
                     TRVSGLLEGEDVIRTAEAARALGASVERLAPGEWRVKGVGVGGLMQP"
     gene            432..812
                     /locus_tag="PLQ11_11040"
     CDS             432..812
                     /locus_tag="PLQ11_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02300 family protein"
                     /protein_id="HRK25477.1"
                     /translation="MAKPELGIKRQCLSCGAKFYDLNRDPILCPKCGAAFQVQQLIRR
                     GETAKAADDADENAADAAGAVMVPLEEADADGAGKDLPGDDIDLGEDLGDDEDDTFLE
                     SEEEEDDGDVSDLIGSDIEDDEES"
     gene            957..1032
                     /locus_tag="PLQ11_11045"
     tRNA            957..1032
                     /locus_tag="PLQ11_11045"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:990..992,aa:Ala,seq:ggc)
     gene            1083..1715
                     /gene="queE"
                     /locus_tag="PLQ11_11050"
     CDS             1083..1715
                     /gene="queE"
                     /locus_tag="PLQ11_11050"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase"
                     /protein_id="HRK25478.1"
                     /translation="MAYSVKELFRTLQGEGANSGRVAVFCRFSGCNLWSGQEKDRAEA
                     VCRFCDTDFVGMDGTGGGRFDCADSLARAIAEVWGEGRQHRFVVLTGGEPLLQVDPPL
                     IDALHRLGFLIAVETNGTIAAPAGLDWICVSPKAGAPLEQTTGDELKLVFPQAGMDPA
                     DFDYLGFRHFYIQPMDGPDRAANTEAAIAYCLAHPHWRLGLQSHKLLGIR"
     gene            1773..2489
                     /gene="queC"
                     /locus_tag="PLQ11_11055"
     CDS             1773..2489
                     /gene="queC"
                     /locus_tag="PLQ11_11055"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HRK25479.1"
                     /translation="MTTTRRALVLFSGGQDSATCLAWALDRFDHVETVGFDYGQRHRI
                     ELTCRAALRAGMARINPAWGPRLGEDHTLNLASLGEVSNTALTRDIAIEMEAGGLPNT
                     FVPGRNIIFLTFAAALAYRRGLRHIVGGMCETDFSGYPDCRDDTIKALQVALNLGMDR
                     RFVLETPLMWIDKAETWHLAETLGGRPLVDLIVTESHTCYLGTRGFPHPWGFGCGTCP
                     ACDLRARGYARFVADGGEAE"
     gene            2486..3490
                     /locus_tag="PLQ11_11060"
     CDS             2486..3490
                     /locus_tag="PLQ11_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRK25480.1"
                     /translation="MSSYVRLGNSGLKVSRLWLGTMMFGLRTQQDEAARIVDAAREAG
                     LNALDTADTYANGASETMLGGLIRKDRSRWVLATKVFNRMGAEPNMAGLSRRWILEEC
                     DHSLRRLGTDWIDLYYLHKDDYDTPLEETVAAIGDLIRAGKIRYFGLSNFRAWRMARV
                     AELCDRMGVPRPIAVQPPYSAVTRGIEVEVLPCAAAYGMGPVVYSPLARGVLTGKYRA
                     GEAPEPETRAARKDERLMQTEFRPESFAIAETLRAHAEATGRTAVDFAIGWVLANRLV
                     NGVIAGPRTLAQWQAYMHAASSPFSAEDEALVDSLVHPGHASTHFYTDPQYPVTGRQN
                     "
     gene            3590..>4147
                     /locus_tag="PLQ11_11065"
     CDS             3590..>4147
                     /locus_tag="PLQ11_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019965617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HRK25481.1"
                     /translation="MLLGVIADDFTGASDIANTIAKGGGGSPGLATVQFLGVPARPAP
                     ASCEAGVVALKSRSIPAQEAIAQSLAALDWLKAQGCRQFVFKYCSTFDSTPEGNIGPV
                     GEALAQRLGVRGVIACPAFPGAGRTIYQGHLFVGDRLLNQSGLENHPLNPMTDADIRR
                     WLGRQTSTPVGLASWNGAVANGAAAL"
BASE COUNT          707 a         1339 c         1361 g          740 t
ORIGIN      
        1 ggctgcatca gcccgccgac cccgacgccc ttcacgcgcc attcgcccgg cgccagccgt
       61 tccacgcttg ctcccagcgc ccgcgcggcc tcggcggtgc ggatcacatc ctcaccttcg
      121 agcaacccgg aaacgcgggt ctcgccgacc gccagcaggc cgaagatcag cgcgcgatgg
      181 gagatcgact tgtcgcccgg cacgcgcacc cggcctcgaa gggaggcgga ccgcccggcg
      241 gtcatcggca ctggaaaaga cgagtgagac acaggttgcc aggctccgga aggttggcga
      301 aagctccggc tcgcctagca tggccatccg cccggcgtca tcctgcctgt tcaaaattct
      361 tcaacgagta tttgacatcg gcgccctgtc cgactaaccg gcaccgaacc tcaaccaacg
      421 ggacgatcga cgtggccaag cccgaactcg gcatcaagcg ccagtgcctc agctgcggcg
      481 ccaaattcta tgacctgaac cgggatccga tcctgtgccc gaaatgcggc gcggcctttc
      541 aggttcagca gctcatccgg cgcggcgaaa ctgccaaggc ggcggacgat gccgacgaga
      601 acgcggcgga tgccgccggc gccgtcatgg tgccgcttga ggaagccgat gcggatggag
      661 ccggcaagga tcttccgggc gatgacatcg atctcggaga ggatctgggc gacgacgagg
      721 atgatacctt cctcgaatcc gaggaagagg aagatgatgg cgatgtctcc gatctgatcg
      781 gctccgacat cgaggatgac gaggagagct gagccgggtt tgcggcctgt gcgggcgggg
      841 gcaagaaaaa ttgtcccgcc cccttgcgaa gctcaggctt ctgtgacatt tgcaccgctc
      901 tcggcgcgag gttccgccga ggcccgcatc ggtccccagc cgttgctcga agatttgggg
      961 ctatagctca gctgggagag cgcttgaatg gcattcaaga ggtcagcggt tcgatcccgc
     1021 ttagctccac caaccttccc cgccgcctga tcgggccagc agggccttag ccggatgcag
     1081 cgatggccta ttcagtcaag gaactgttcc gaacattgca gggagagggc gccaacagcg
     1141 ggcgcgtcgc cgtattctgc cgtttttccg gctgcaacct gtggtctggt caggaaaagg
     1201 accgggccga agccgtatgc cggttctgcg ataccgattt tgtcggcatg gacggcaccg
     1261 gcggcggccg cttcgattgc gcggattcgc tggcgcgcgc cattgccgag gtctgggggg
     1321 aagggcgcca gcaccgtttt gtcgttctga ccgggggcga gccgctgttg caggtcgatc
     1381 cgccgctgat cgatgcgctg caccgtctgg gttttctgat cgccgtggaa accaacggca
     1441 cgattgccgc gccggctggc cttgactgga tctgcgtcag tcccaaggcc ggcgctccgc
     1501 tggaacagac cacgggcgac gagttgaagc tggtctttcc gcaagccggc atggaccctg
     1561 cggatttcga ctatctcggc ttccgtcatt tctatatcca gccgatggac gggccggatc
     1621 gcgccgccaa caccgaggct gctatcgcct attgcctggc ccatccgcac tggcggcttg
     1681 gcctgcaatc gcacaagctt ctgggtatcc gatagggccg ttgacgctgc cggccggcac
     1741 cgcgcacagt aatgcgacag ggaacgggaa ccatgacgac aacgaggcgc gcgctggtgt
     1801 tgttttcggg cgggcaggat tcggcaacct gccttgcctg ggcgcttgac cgtttcgatc
     1861 atgtcgagac cgtcggcttc gactacggcc agcgccaccg catcgagctc acctgccgtg
     1921 ccgccctgcg cgccggcatg gcaaggatca atccggcatg ggggccgcgg ctcggcgagg
     1981 atcacacatt gaatctcgcc agcctcggcg aggtgtcgaa tacggctttg acgcgtgaca
     2041 tcgccatcga gatggaggcg ggcgggctgc ccaacacttt cgttcccggc cgcaacatca
     2101 tttttctcac cttcgcggcg gcactcgcct atcgtcgcgg cctgcgccac atcgtcggcg
     2161 ggatgtgtga aaccgacttc tccggttatc ccgattgccg ggacgatacg atcaaggctc
     2221 tgcaggtagc gctcaatctc ggcatggacc gccgcttcgt gctggagacg ccgctgatgt
     2281 ggatcgacaa ggcagaaacc tggcatctgg cggaaacgtt gggcggccgg ccgctggtcg
     2341 atctgatcgt cactgagagc catacctgct atctgggaac gcgcgggttc cctcacccct
     2401 ggggatttgg ctgcggcacc tgcccggcct gtgacctgag ggccaggggc tatgcccgct
     2461 ttgttgcgga cgggggagag gcagaatgag cagctatgtc aggcttggaa acagcggctt
     2521 gaaggtttcg cgcctgtggc tgggcacgat gatgttcggc ctgcggacgc agcaggacga
     2581 ggctgcccgg atcgtcgatg cggcgcgcga ggcaggcctc aatgcgcttg ataccgcaga
     2641 cacctatgcc aatggcgcct ccgagaccat gctgggcggc ctgatccgca aggatcgctc
     2701 ccgctgggtg ctggccacca aggtgttcaa ccgcatgggg gcggagccga atatggccgg
     2761 cctgtcgcgg cgctggatcc tggaggaatg cgaccattcg ctgcgtcgcc tcggcaccga
     2821 ctggatcgac ctctactatc tgcacaagga cgattatgac acgcccctcg aagagaccgt
     2881 ggcggcgatc ggcgacctga tccgcgccgg caagatccgc tatttcggcc tgtcgaattt
     2941 ccgcgcctgg cgcatggcgc gcgtggccga gctgtgtgat cggatgggag tgccacgtcc
     3001 gattgccgtg cagccgccct acagtgcggt gacccgcggc atcgaggtcg aggtcctgcc
     3061 ctgcgctgcg gcctatggca tggggccggt ggtctattct ccgctggccc gcggcgttct
     3121 gaccggaaaa taccgggcgg gcgaggcgcc cgagccggaa acgcgcgccg cccgcaagga
     3181 tgagcgtctg atgcagaccg aattccgccc tgaatccttc gcgattgccg agaccctgcg
     3241 cgcccatgcc gaagccaccg ggcgaacggc ggttgatttt gccatcggct gggtgctggc
     3301 caaccgactt gtcaacggcg tcattgccgg cccgcgcacg cttgcccaat ggcaggccta
     3361 tatgcacgcc gcatcaagcc cgttctccgc cgaggatgag gcattggtcg attctctggt
     3421 gcatcccggc catgcctcca cccatttcta tactgatccg caatatccgg tgaccgggcg
     3481 gcagaactga ggccgtcctc gccgggggct ggacgggcgg ttgccagcca ccagcgtcgg
     3541 cgctacgcag gtcatcagac caccgaacgg caagacggac ggagtgacca tgcttctggg
     3601 tgtgattgcc gacgatttca ccggcgcgtc cgacatcgcc aacacgattg ccaaaggcgg
     3661 cggcggttcg cccgggctgg cgacggtgca gtttctcggt gttccagcca ggcccgcgcc
     3721 ggcaagctgc gaggccggcg ttgtcgcgct gaaaagccgg tccattccag cgcaggaagc
     3781 catcgcccaa tcgctcgccg ctcttgactg gctgaaggca cagggctgcc ggcaatttgt
     3841 gttcaaatac tgctcgacct tcgattccac cccggagggc aatatcggcc cggtgggcga
     3901 ggcgctggcg cagcggctcg gcgttagggg cgtgatcgcc tgcccggcct ttcccggcgc
     3961 ggggcggacg atctatcagg gccatctctt cgtcggcgac cggctgctca accagtcggg
     4021 tctcgagaac catccgctca acccgatgac cgatgcggat attcgtcgct ggctcggccg
     4081 gcagaccagc actccggtgg ggctggcaag ctggaacggc gccgtcgcca acggcgccgc
     4141 cgcgctg
//
LOCUS       DAOMXG010000194         4145 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_11627_length_4145_cov_9.035697, whole genome shotgun sequence.
ACCESSION   DAOMXG010000194 DAOMXG010000000
VERSION     DAOMXG010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4145
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11627_length_4145_cov_9.035697"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PLQ11_11070"
     CDS             <1..206
                     /locus_tag="PLQ11_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRK25482.1"
                     /translation="ASVIEDVVRPDAAGRQLLKDASERFRLTARGYHRVMKVARTLAD
                     LEGAADVGRRHLAEALAYRPRLD"
     gene            complement(240..1637)
                     /locus_tag="PLQ11_11075"
     CDS             complement(240..1637)
                     /locus_tag="PLQ11_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRK25483.1"
                     /translation="MSGEAGFRRPGLSGRLMMLTLGFVMLAEVLIFVPSIANFRNTWL
                     RDRLAAARTAALVLEASPSQSLPDALVAQLLDSVGSRTIVHKTGSVRRLLAVSDMPLD
                     VSRDLTLVDTPNIETVFEAFDTLLFGGQRLVRVMGPKSPGGDMIEIVLDETPLRTAML
                     NYSRTILLLSLIISSITAGLVFLSLHRLIVRPVKRLAESISSFAQDAEDSRRVIVPSG
                     RSDEIGGAEESLAGMQRTLNQQLRQQQHLAALGLAVAKINHDLRNMLSSAHLLSDRLS
                     MVRDPTVQRIAPKLIRALDRAIGFCQATLAFGRAQEQPMLMREVELAPLVQDLRDLIG
                     ITEDSVVDFVAEVPPRLSVSADPDQLLRVLLNISRNAMEAMAGNAVPARPARLTVSAR
                     AVAGQVEIDIADTGPGIPKEARANLFKPFAGSNRAGGTGLGLAIAAELMKAQGGSVTL
                     VAVTDGTCFRISLPA"
     gene            complement(1634..3079)
                     /locus_tag="PLQ11_11080"
     CDS             complement(1634..3079)
                     /locus_tag="PLQ11_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008552438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRK25484.1"
                     /translation="MAKTTNSKTLPRPDLCVIGAGAAGLSVAASAALMGAQVVLIEQA
                     RMGGDCLNSGCVPSKALIAAARAAQGVREAGRFGISARAPGVDRAAVKRHVDAVIAEI
                     APVDSVARYRALGAEVIEGEARFVDPSTVMVGERTIKARRFVIASGAAATVPPIPGLD
                     DVPYLTHETIFALTETPKRLAIIGGGPIGIELAQAHRRLGSDVTVFEAERILPREDVD
                     AARLIARVLEREGVRLIEYARIRAVSGGAGAIRIDVEGEHLRKGQTFTHLLVATGRAP
                     RVEGLGLEAAGVRYTARGIEVDQSQRSSNPRIYAIGDCAGGAQFTHAASHQAGIVIRN
                     ALFRLPAKRQDSLIPRVTFTDPEIAAVGLNEDSAQASGARGVTALRWPLAENDRARID
                     GATDGFAKIVTDGRGRILGVTLAGGHAGEQINLWTLAMRQKLTVSAIIDLVMPYPTYS
                     EAARRAALNSLTPKLRSPWIGRMIRLMRVFG"
     gene            3395..3529
                     /gene="rpmH"
                     /locus_tag="PLQ11_11085"
     CDS             3395..3529
                     /gene="rpmH"
                     /locus_tag="PLQ11_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010659431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HRK25485.1"
                     /translation="MKRTYQPSKLVRKRRHGFRSRMATAGGRKVIAARRARGRKRLSA
                     "
     gene            3544..3951
                     /gene="rnpA"
                     /locus_tag="PLQ11_11090"
     CDS             3544..3951
                     /gene="rnpA"
                     /locus_tag="PLQ11_11090"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HRK25486.1"
                     /translation="MPGPEAEGEPACRRSSGQVPTLKHRRDFLLLARGVRRHARAFSL
                     QVGPAEEAIGRIGYTLTRKVGTAVERNRIRRRLRAAVGAVVGLNPMAFDAVLIGRREG
                     LHEPFDTLCSALAKELASASRRLPATPVDAPGV"
BASE COUNT          703 a         1383 c         1337 g          722 t
ORIGIN      
        1 ccgcctcggt catcgaggat gtcgtacgcc ccgatgccgc gggccgccag cttctcaagg
       61 atgcttccga acgctttcgc ctgactgccc gtggctatca ccgggtgatg aaagtggcgc
      121 ggaccttggc ggatcttgag ggagccgccg acgttggccg gcggcatctg gccgaggcgc
      181 tggcctatcg cccgcggctg gactgacccc cacggggcgg gcggcaggcc tgtggggcat
      241 caggctggca gcgagatacg gaagcaggtt ccatccgtaa cggcgacaag ggtgacactg
      301 ccgccctgtg ccttcatcag ttcggcggcg atcgccagtc cgagccctgt gccgccggcc
      361 cggttcgatc cggcaaaggg cttgaacagg ttggcgcggg cctctttcgg gatcccgggg
      421 ccggtatcgg cgatgtcgat ttccacctgc ccggcgaccg cgcgggcact gaccgtcagc
      481 cgtgccggac gggcggggac ggcattgccg gccatggcct ccatcgcatt gcggctgatg
      541 ttcagcagaa cacgaagcag ctggtccgga tcggccgaga cactcaggcg gggcggaact
      601 tcggcgacga agtcgaccac actgtcctcg gtgatgccga tgagatcgcg cagatcctgc
      661 accagcgggg ccagttccac ctcgcgcatc agcatgggct gttcctgcgc ccggccgaaa
      721 gccagcgtcg cctgacagaa cccgatcgcc cggtcgagtg cgcggatcag tttgggggct
      781 atgcgctgca ccgtaggatc gcgcaccatc gacagccggt cggacaacag atgagcactc
      841 gacagcatgt tgcgcaggtc gtgattgatc ttggcgaccg ccagccccag ggctgccaga
      901 tgttgctgct gccggagttg ctggttgagg gtccgctgca tgccggcgag cgattcctcg
      961 gcgccgccga tctcgtcgct gcgccctgac ggcacaatca ccctccggct gtcctcggca
     1021 tcctgggcga aggacgagat gctttccgcc agccttttga ccggccgcac gatgaggcga
     1081 tgcaggctga gaaagacaag gccggcggtg atcgacgaga tgatgaggga tagcaggagg
     1141 atcgtgcgcg agtagttcag catcgctgtg cgcagcggcg tctcatcgag cacgatctcg
     1201 atcatgtcgc cacccgggct cttcggcccc atcacgcgca ccagcctctg gccgccgaac
     1261 agcagcgtat cgaacgcctc gaacaccgtc tcgatattgg gagtatcaac cagggtcaga
     1321 tcgcgcgaaa catcgagcgg catgtcggaa accgcgagca acctgcggac actgccggtc
     1381 ttgtgcacga tggtccgcga accgacgctg tcgagcaact gtgccaccag cgcgtccgga
     1441 agggattggc tggggctcgc ctccagcacc agcgcggccg tgcgcgcggc cgccagccga
     1501 tcgcgcagcc aggtgttgcg gaaattcgcg atcgagggaa cgaagatcag cacttcagcc
     1561 agcatgacga agccgagcgt cagcatcatc aggcgcccgg agaggccagg ccgcctgaaa
     1621 cctgcttcgc cgctcatccg aacacccgca tcagcctgat catccgcccg atccagggcg
     1681 agcgcagctt gggtgtcagg ctgttgagag cggcgcggcg cgcagcttcg gaataggtcg
     1741 gatagggcat gacgaggtcg atgatcgccg aaacggtgag tttctgccgc attgccagtg
     1801 tccacaggtt gatctgttcg ccggcatgcc ctccggccag cgtgacaccc agaatgcggc
     1861 cccggccatc tgtaacgatc ttggcaaaac cgtcggtagc gccgtcgatg cgtgcccggt
     1921 cattctctgc cagcggccag cgcagggcgg tgacgccgcg ggcgccgctt gcctgggcgg
     1981 agtcctcgtt gaggccgacg gcagcgattt cgggatctgt aaaggtcacg cgcggaatca
     2041 gggaatcctg ccgtttggcc ggcaggcgga acagggcgtt gcggatcacg atacccgcct
     2101 gatggctcgc cgcatgggtg aattgcgctc cgccggcgca atcgccgatc gcatagatgc
     2161 gcgggttgga gctgcgctgg ctctggtcaa cttcgatccc gcgcgcagtg tagcggacgc
     2221 ccgccgcctc aagcccgagg ccttcgacgc gcggtgcccg ccccgttgcg accaatagat
     2281 gggtgaacgt ctgccctttc ctgaggtgct cgccctcgac gtcgatgcgg atcgcgccgg
     2341 caccgcccga cacggcgcgg atgcgggcat attcgatcag gcggacgcct tcgcgctcca
     2401 gcacccgggc gatcagccgg gcagcatcga catcctcgcg gggcaggatg cgttctgcct
     2461 cgaacaccgt cacatccgag ccgagccgcc gatgggcctg ggccagctcg ataccgatcg
     2521 gcccgccacc gatgatggcc agccgctttg gcgtctcggt cagcgcaaaa atcgtctcgt
     2581 gggtaagata gggcacatcg tccagccccg ggatcggcgg caccgtcgcc gccgcaccgc
     2641 tggcgatgac gaacctgcgt gccttgatcg ttcgttctcc aaccatgacg gtggacggat
     2701 cgacaaagcg ggcctctccc tcgatcacct cggcccccag cgcccggtag cgcgcaaccg
     2761 aatcgaccgg cgcgatctcg gcgatcaccg catcgacatg acgcttgacc gccgcccgat
     2821 caacgcccgg cgcccgtgct gatatgccga acctgcctgc ctcgcgtacg ccctgtgctg
     2881 ctcttgccgc agcaatcagc gccttggaag gaacgcagcc tgaattgagg cagtcaccgc
     2941 ccatacgggc ttgctcgatc agcacgacct gcgcgcccat cagcgcggca gaggccgcca
     3001 ctgacaatcc tgcggctccg gcgccgatca cgcacaggtc cggtctcggc agggtcttgc
     3061 tattcgttgt tttggccatc ccgtccgtca ccttggccgc cccgcgcgcc ggagcgtgcc
     3121 gccgcgcatc gctttgcgca tgataaacga caaaatcatg acgcaacccg ccgccgcaag
     3181 ccagcccgcc agcctttttc acctttggcg accgctctga aggtagcgat caattcgggc
     3241 gtcagtgaag gttggatcag caattgactt ttacccaggt tcccgtttaa agctcgccgc
     3301 aaattacgcg tcctgtcgga tgcagcagcg gccggcgcca ttctggcgcc gcttttgttg
     3361 ccgacagtga caggaacccg atcggagttg tatcgtgaag cgcacctatc aaccgagcaa
     3421 gctggtccgc aagcgtcgtc acggctttcg ctcgcgcatg gctaccgcgg gtggccgcaa
     3481 ggtgatcgcc gcgcggcgtg cgcgcggtcg caagcgcctt tcggcgtaag gcggtttttt
     3541 ctcatgcctg gaccggaggc ggagggcgaa ccggcctgcc gtcgcagttc cgggcaggtg
     3601 ccgacattaa agcatcggcg ggattttctg ctgttggcca ggggcgtgcg ccgtcatgcg
     3661 cgcgcctttt cgttgcaagt tggtccggcc gaagaggcca tcgggcgtat cggctatacc
     3721 ctgacgagga aggttggcac tgcggttgag cgcaaccgca tccgccgccg tctgcgcgcc
     3781 gccgtcggcg ctgtcgtggg cctcaatccg atggccttcg atgccgtgct gatcggtcgg
     3841 cgcgaaggat tgcacgaacc ctttgacacc ctctgctcgg cgctggcaaa ggaactggcc
     3901 agtgccagtc gccgtcttcc agccaccccg gtggacgccc caggcgtctg agctttccgt
     3961 caggactatt ccaccatgca gccgcatccc tccgacaaca agaaccttct gctcgccatc
     4021 gtcctgtcgg tcatcgtgat ggtcggctgg aactatttct atggcgtgcc gcagatggac
     4081 aagtccaagc aggccgcgca gaacgccctt gttcagcccc agcaacaggg cgccgcgccg
     4141 acggc
//
LOCUS       DAOMXG010000195         4081 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_11896_length_4081_cov_11.119722, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000195 DAOMXG010000000
VERSION     DAOMXG010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11896_length_4081_cov_11.119722"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="PLQ11_11095"
     CDS             <1..606
                     /locus_tag="PLQ11_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HRK25487.1"
                     /translation="GARPQEIDAALEAYGFAMGPFAVQDLAGLDIAWARRKRLAAMRP
                     AAERYVPIADQLCEAGRFGQKTGAGWYRYENGKRQVDPAVTDLVVAHSQNRGIVRHPI
                     LPEEIQARVLAAMVNEGAKIVAEGVADRPSDIDVVLINGYGYPAWRGGPMHEADRTGL
                     PTILKTLKPMQGRDGPAFEASALLIEVAAEGGTLGQLNSDP"
     gene            691..2001
                     /gene="hmgA"
                     /locus_tag="PLQ11_11100"
     CDS             691..2001
                     /gene="hmgA"
                     /locus_tag="PLQ11_11100"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HRK25488.1"
                     /translation="MSERYMSGFGNGFETEALPGALPIGRNSPQRCAYGLYAEQLSGS
                     PFTAPRASNERSWLYRIRPTVKHWGRFRKADSGLWRTAPAVEVAAPPAPYRWSPIPLP
                     AERVSFLEGVRTITTAGDANATAGMSASIYLVTRSMTDKCFYNADGELLFVPENGGLR
                     LVTEFGIIDVAPGEIAVIPRGIKFRVELPEGQKAARGYLCENYGGAFTLPERGPIGAN
                     CLANQRDFYSPVARYEEVDRPTAMYVKWGGSLWVTELQHSPLDVVAWHGNYAPYKYDL
                     RRFSPVGPLLFDHADPSIFTVLTSPSETAGTANIDFVCFPERWMVAEDTFRPPWYHMN
                     CMSEFMGLIYGQYDAKPEGFVPGGFSLHNTMLPHGPDVDSFEKNSTVPLRPHKLENTM
                     AFMFETRFPQQITAYAMNAPELQDSYFDYGHALKDHFDPRRRDR"
     gene            2014..3039
                     /locus_tag="PLQ11_11105"
     CDS             2014..3039
                     /locus_tag="PLQ11_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008598308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRK25489.1"
                     /translation="MKLATLKDGSRDGRLVVVSRDLTRCTDAAVICRTLQQALDGWER
                     YAPALADLAEQLDHGSVPSQRFHEHDCESPLPRAYQWADGSAYVNHVELVRKARGAEM
                     PPSFWTDPLMYQGGSDSFLPPRDPILMPTEAFGIDLEAEVAVVTGDVPMGVTPEAARA
                     AIRLVMLVNDVSLRNLIPNELAKGFGFFQSKPSSSFAPVAVTPDELGDAWKGGKLHLP
                     LLSFLNGKPFGKPDAGVDMTFDFGMLIAHAAKTRPLAAGTIIGSGTVSNKGADGGPGT
                     PISAGGVGYSCLAELRTVETLTNGKPTTPFLAFGDTVRIEMRDRKGHSIFGAIEQTVQ
                     RQDGPAE"
     gene            3061..4020
                     /locus_tag="PLQ11_11110"
     CDS             3061..4020
                     /locus_tag="PLQ11_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRK25490.1"
                     /translation="MSGFASTADMTPKKVSFKKLGDGLYAYTAEGDPNTGVIIGDDGV
                     MIVDAQATPAMAQDVIAKVRSVTDKPIRYVLLSHYHAVRVLGASAYKDAHAILASDET
                     RKLIVERGAQDMASEIGRFPRLFQGKETIPGLTWPTLTFTGEISVWLGKREVRISQPG
                     RGHTAGDTIAYVPDAGVLFSGDLVEFRSACYCGDAHYEDWPKTLRKLKKYDPVAVVPG
                     RGDALIGRPNVAAAFTGTHNFLTTLYSTVQAAVERGETLKQAYDQVRRVMDEPFGHYA
                     IYEHCLPFNVSRAYDEARGIDHPVIWTAERDVAMWKALEKGQS"
BASE COUNT          762 a         1284 c         1267 g          768 t
ORIGIN      
        1 ggcgcccggc cgcaggagat cgacgccgcg ctcgaagcct atggctttgc catggggccg
       61 ttcgcggtgc aggatctcgc cggtctcgat atcgcctggg cgcgacgcaa gcgcctcgcc
      121 gcgatgcggc cggccgccga gcgctacgtg ccgattgccg atcaactctg cgaggccggc
      181 cgcttcggcc agaagaccgg cgcgggatgg tatcgctacg aaaacggcaa gcgccaggtc
      241 gacccggccg tgaccgacct tgttgtcgcc catagccaga atcgcggcat cgtccggcac
      301 ccaatcctgc ccgaggaaat ccaggcccgt gttctggctg ccatggtcaa cgagggtgcc
      361 aagatcgtcg ccgaaggtgt cgctgaccgc ccttccgaca tcgatgtcgt gctgatcaac
      421 ggctacggct atccggcctg gcgcggcggg cccatgcacg aggcggatcg caccggcctg
      481 ccgaccatcc tcaagaccct gaaaccgatg cagggtcggg acggcccggc gtttgaagct
      541 tctgcactgc tcatcgaggt tgcagcagaa ggcggcacgc ttggccagtt gaacagcgac
      601 ccctgagagc gggacattga cagaggtgcg gctggtccct aagacaatcc aattagtaac
      661 ttttgttact attttcagga agaggagccc atgagcgagc gttacatgtc cggcttcggc
      721 aacgggtttg aaaccgaggc gctgccggga gcgttgccga tcggccgcaa ttcgccgcag
      781 cgctgcgcct atggccttta tgccgaacaa ttgtcgggat cacccttcac cgcgccgcgc
      841 gccagcaacg agcgttcctg gctttaccgc atcaggccga cggtcaagca ctggggtcgc
      901 ttccgcaagg ctgatagcgg cctgtggcgg acggcgccgg cggttgaggt tgcggctcct
      961 cccgcgccgt atcgctggtc ccccattccc cttcccgccg agcgcgtctc tttccttgag
     1021 ggcgtgcgca ccatcacaac ggcaggcgat gccaacgcca ctgccggcat gtcggcttcg
     1081 atctacctcg tgacccgatc gatgacagac aaatgcttct acaatgccga tggcgaactg
     1141 ctgttcgttc cggaaaacgg tggtttgcgg cttgtcaccg aattcgggat catcgacgtt
     1201 gcccccggcg agatcgctgt cattccgcgc ggcatcaagt tccgggttga actccccgaa
     1261 ggccagaagg cggcgcgcgg ctatctctgc gagaattatg gcggcgcctt caccctgccc
     1321 gagcgcggac cgatcggcgc aaactgcctc gccaaccagc gcgacttcta ttcgcctgtc
     1381 gcccggtatg aggaggtcga tcggccgact gccatgtacg tcaagtgggg tggctcattg
     1441 tgggtaaccg agctccagca ctcgccgctc gatgtggtgg cctggcatgg caattatgcg
     1501 ccctacaaat atgacctcag gcggttttcg ccggtcggac cgctactgtt cgatcatgcc
     1561 gatccgtcga tcttcactgt gctgacgagc ccgtccgaga ccgccggaac cgccaatatc
     1621 gactttgtct gcttcccgga gcggtggatg gtcgccgaag atacctttcg cccgccgtgg
     1681 tatcacatga actgcatgag cgaattcatg gggctgatct acggccagta cgatgccaag
     1741 cctgaagggt tcgtgcccgg cggcttttca ctgcacaaca ccatgctgcc gcacggtccg
     1801 gatgtggaca gtttcgaaaa gaattcgacg gttcccctcc ggccgcacaa gcttgaaaac
     1861 acgatggcct tcatgttcga gacgcggttc ccacaacaga tcactgctta cgcgatgaat
     1921 gctcccgaat tgcaggacag ctatttcgac tacggccacg cgctgaagga tcatttcgat
     1981 ccccgtcgcc gcgacagatg aggactggca cagatgaaac ttgcaacgtt gaaggatgga
     2041 tcacgcgacg ggcggctcgt cgtcgtatcg cgcgatctga cgcgctgcac ggatgcagcc
     2101 gtgatctgtc gcaccctgca acaggcgctc gacggctggg aacgctatgc cccggcgctg
     2161 gccgacctgg ccgagcagct cgatcatgga tccgtgccat cgcagcgttt ccacgaacat
     2221 gattgcgaaa gcccgctgcc gcgcgcctat cagtgggcgg atggatcggc ctacgtcaac
     2281 cacgtcgagc tggtgcgcaa ggcgcggggc gccgagatgc cgccgtcgtt ctggaccgac
     2341 ccgctgatgt atcagggcgg ctccgacagt ttcctgccgc cgcgcgatcc gatcctgatg
     2401 ccgacagaag cgttcggaat tgacctagag gcagaggttg ccgtggtgac cggcgacgtg
     2461 ccgatgggcg tgacacctga ggcggcccgc gcggccatcc gcctcgtcat gctggtcaat
     2521 gatgtctccc tgcgcaacct gatccccaac gagctggcca agggatttgg cttcttccag
     2581 tccaagccat cctcctcgtt tgctccggtg gccgtgaccc ccgatgaact gggcgatgcg
     2641 tggaagggcg gcaaactgca tctgccgctg ctgtcgttcc tcaacggcaa gccattcggc
     2701 aagcccgatg ccggtgtcga catgaccttc gacttcggga tgctgattgc ccatgcggcc
     2761 aagacgcggc cacttgccgc cggaacgatc atcggctccg gcacggtctc caacaagggg
     2821 gctgatggcg ggcccggcac gccgatctct gcgggcggcg tcggctattc ctgcctagcg
     2881 gagttgcgta ccgtcgaaac gctcaccaac ggcaagccga ccacgccatt cctggccttc
     2941 ggcgataccg tgcgcatcga gatgcgggac agaaagggcc attccatctt cggagcaatc
     3001 gagcagacgg tgcagcgcca ggatggccca gccgaataat cagtattgtg aggatctgac
     3061 atgagcggtt ttgcctcgac ggccgacatg acgccgaaga aagtgtcttt caagaagctg
     3121 ggcgacgggc tctatgccta tacggcggag ggcgatccca acaccggcgt catcatcggc
     3181 gatgacgggg tgatgatcgt cgatgcgcag gcgaccccgg cgatggcgca ggacgtgatc
     3241 gccaaagtgc gctcggtcac cgacaagcca atccgctatg tgctgctgtc gcattatcat
     3301 gccgtgcgtg tgctgggcgc ctcggcctac aaggatgcgc acgccatcct tgcctcggac
     3361 gaaacccgaa aactcatcgt cgagcgtggc gcgcaggaca tggcttcgga gataggccgg
     3421 tttccgcggc tgttccaggg caaggaaaca atcccaggcc tgacctggcc aaccctcacc
     3481 ttcaccggcg aaatctctgt ttggctgggc aagcgtgagg tgcgtatttc acagcctggg
     3541 cgcggccata cggcaggcga cacgatcgct tatgtgccgg acgccggggt gctgttctcg
     3601 ggcgatctcg tcgagttccg ctctgcctgc tactgcggag acgcccatta tgaagactgg
     3661 cccaagacgc tgagaaagct gaagaagtat gaccccgttg ctgtcgtgcc ggggcgtggc
     3721 gacgccctga tcggcaggcc gaatgtcgcc gccgccttca ccggaacgca caatttcctg
     3781 accacgctgt attcgaccgt ccaggctgcc gtcgagcggg gcgaaacgct gaaacaggcc
     3841 tatgatcagg tgcgcagggt aatggacgaa cccttcgggc attacgcgat ctatgagcac
     3901 tgcctgccgt tcaacgtctc tcgcgcctat gacgaggcca gggggatcga tcacccggtg
     3961 atctggacgg ccgagcgcga tgtcgccatg tggaaggcgc tggaaaaagg ccagagctga
     4021 ccaacctagg tcgtagttac catttaacga ttgcctggac gcgagggatt ttggctcaag
     4081 g
//
LOCUS       DAOMXG010000196         4029 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_12113_length_4029_cov_9.396074, whole genome shotgun sequence.
ACCESSION   DAOMXG010000196 DAOMXG010000000
VERSION     DAOMXG010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12113_length_4029_cov_9.396074"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            300..1037
                     /gene="fabG"
                     /locus_tag="PLQ11_11115"
     CDS             300..1037
                     /gene="fabG"
                     /locus_tag="PLQ11_11115"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014285323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HRK25491.1"
                     /translation="MFNLEGKCALVTGASGGIGAGIAAALHAQGATVTLSGTRRDALD
                     GIAAKLGQRAHVVTANLSDRASLEALLPSVEAAMGKVDILVNNAGITRDNLFMRMKDE
                     EWDSVLAVNLTAGFVLARAAVKGMMRRRFGRIIGITSIVGVTGNPGQANYAAAKAGMI
                     GMSKALAMEIASRGITVNCIAPGFIETPMTDALNDKQREAILKDIPASRLGSVGEIAA
                     AAVYLASEEAAYVTGQTLHVNGGMARI"
     gene            1252..1488
                     /locus_tag="PLQ11_11120"
     CDS             1252..1488
                     /locus_tag="PLQ11_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018062986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRK25492.1"
                     /translation="MATIAERVKKIVIEHLSVDADKVVEKANFIDDLGADSLDTVELV
                     MAFEEEFGVEIPDDAAETIVTVGDAIKFLEKNAA"
     gene            1528..2796
                     /gene="fabF"
                     /locus_tag="PLQ11_11125"
     CDS             1528..2796
                     /gene="fabF"
                     /locus_tag="PLQ11_11125"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HRK25493.1"
                     /translation="MSGLRRVVVTGLGMVSPHGCGVEPTWSRILAGESAARKIERFDV
                     SDISCKIGCPIPRGTGAGEFNPDDWMEPKEQRKVDDFIIFALAAARQGLDDAGWHPTS
                     YEDQIASGVLIGSGIGGIYGISEASLILRDKGPRRLSPFFIPGRLINLAGGYVSIEHG
                     LKGPNHSVVTACSTGAHAIGDAARLVAMGDADVMVAGGSESPLERISLAGFAACRALS
                     TSFNDEPHRASRPYDKDRDGFVMGEGAGVVVLEALDHALARGAKIYAEVIGYGLSGDA
                     HHITSPSPDGDGAYRCMAAALKRAGITAADVDYVNAHGTSTPVGDEIELGAVARLVGN
                     CAANLSMSSTKSAIGHLLGAAGAVEAIFSILAIRDQIAPPTLNLDNPSVETPIDLVPH
                     KARPRKIDVALSNSFGFGGTNASLVFRRYA"
     gene            2870..>4029
                     /gene="mltG"
                     /locus_tag="PLQ11_11130"
     CDS             2870..>4029
                     /gene="mltG"
                     /locus_tag="PLQ11_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRK25494.1"
                     /translation="MADNSNPPEEKQTRRRSLFSPRDKLKPVAAPPPPPRPKRKQGGM
                     IAVLSAVLTVSVIILVAATAGIGYVKRMVASPGPLQTEKSIVIERGAGAEEIAELLER
                     EGIITQPTLFQVLAMSQVYGRLKAGEFLFKREASLSDVIDTIANGRAIEHTITIPEGL
                     TSEQIVARLKESDLLTGEVASVPREGALLPDTYRIARGTSRDSLLARMEKEQKRVLGE
                     VWNKRAKDVPLKSPSDLVILASVVEKETGKADERSRVAGVFVNRLNRNMRLQSDPTIV
                     YGLVGGKGTLGRGITRADIQSNTPYNTYVINGLPPGPIANPGRASLEAAANPSRTREL
                     FFVADGTGGHAFSETLEQHERNVARWRQIEAQRDSGAAPGFGAVPLAPGAAPS"
BASE COUNT          714 a         1271 c         1350 g          694 t
ORIGIN      
        1 gcaactgctg gcaggtgcgc gcctcggcgg cggccgagac cagaagtccg cccaccagcg
       61 ccacgccggc cagcgacagg ttgaaccatt gacggcgttg catgttcggc tccctcggtt
      121 gccacgttta tcagcaaatt ctaatccgcc gccggctcgc cgccatgata tggcgcaaca
      181 gaatcccgcc ctgcgggagt agactggggg aggggtgttt ccaccgcagc gcttgacttt
      241 ggcgcgcaca tgccgtcgat ggcgcggcca atgtgtttgc ggcagatgcg ggggaggcca
      301 tgttcaatct ggaaggcaaa tgcgcgctgg tgaccggtgc gtcggggggg atcggcgccg
      361 gcattgccgc tgcgctgcat gcgcagggcg cgaccgtgac cctgtccggc acgcggcgcg
      421 atgcgctcga cgggatcgcc gcaaagctcg gccaacgcgc acatgttgtg accgccaacc
      481 tgtcggatcg cgcttcgctc gaagcgctgc tgccgtccgt cgaggcggcg atgggcaagg
      541 ttgacattct cgtcaacaac gccggcatca cccgcgacaa cctgttcatg cggatgaagg
      601 acgaggaatg ggacagcgtg ctggcggtca acctgaccgc cggcttcgtg ctggctcgcg
      661 ccgccgtgaa aggcatgatg cgccgccgct ttggccgaat catcggcatt acctcgatcg
      721 tcggcgtcac cggaaatccg ggccaggcca attacgccgc cgcaaaggcc gggatgatcg
      781 gcatgtcgaa ggcgctggcg atggagatcg cctcgcgcgg gatcacggtg aactgcatcg
      841 cccccggatt catcgagaca ccgatgaccg acgcgctcaa cgacaagcag cgcgaggcca
      901 tcctcaagga tatcccggcc agtcgcctgg gaagcgtcgg cgaaattgcc gccgccgccg
      961 tgtatctcgc cagcgaagag gccgcctatg tgaccggcca gacgctgcat gtgaacggcg
     1021 gcatggcaag aatctgaccc ctgccgagaa aaaaccgtgg tgggcagcca caccacaacc
     1081 ttgaggactg ttgactttct cgcaggttct aacaaaggaa gggcgacact ccggcagttg
     1141 cgtgccgtca gcgggacgca gccgggtgcg tttgtgagta aattggttca gtcggtggcc
     1201 cgatccggac aacgacttga aggaccaggg tctaacgagg gaattcctat catggccact
     1261 atcgctgagc gcgtgaagaa gatcgtcatc gagcacctga gcgtggatgc tgacaaggtt
     1321 gtcgagaagg cgaacttcat cgacgatctg ggcgcggaca gcctcgacac cgtggaactg
     1381 gtgatggcat tcgaggaaga attcggcgtc gagattccgg acgatgcggc cgagaccatc
     1441 gtcacggtgg gcgacgcgat caagttcctg gagaagaacg cggcctgagc ctccggctcg
     1501 ggacccgtgt tttcatcagg atcggatatg tccggattac gtcgcgttgt cgtgactggc
     1561 ctcggcatgg tctctccgca tggctgcgga gtggaaccga cgtggtcgcg catcctggcg
     1621 ggcgagagtg ccgctcgcaa gatcgagcgc ttcgatgtct ccgacatatc ctgcaagatc
     1681 ggctgcccga tcccgcgcgg aaccggcgcc ggggagttca accccgacga ctggatggag
     1741 cccaaggagc agcgcaaggt cgatgatttc atcatcttcg ctcttgccgc tgcccgccag
     1801 gggctcgacg acgcgggctg gcacccgacc agttacgagg atcagatcgc atccggcgtg
     1861 ctgatcggct cggggatcgg cggcatctac ggcatttccg aagcgtcgct gatcctgcgc
     1921 gacaaggggc cgcggcggct gtcgccgttt ttcattcccg gccggctgat caatctggcc
     1981 ggcggctatg tttccatcga gcacgggctc aaggggccca accattcggt ggtcaccgcc
     2041 tgttcgaccg gcgcgcatgc gatcggcgac gcggcccggc tggtggcgat gggcgatgcc
     2101 gatgtcatgg tggccggcgg ttcggaatcg ccgcttgagc gcatctctct tgccggattt
     2161 gcggcctgcc gggcgctttc gaccagtttc aacgacgagc cgcatcgcgc ctcgcgcccc
     2221 tatgacaagg atcgcgacgg gttcgtgatg ggcgagggcg ccggtgtcgt ggtgctcgag
     2281 gcgctcgatc acgcgctggc tcgcggcgcg aagatctatg ccgaggtgat cggctacggc
     2341 ctgtcgggcg atgcccacca catcacctcg ccatcgccgg atggcgacgg cgcctaccgc
     2401 tgcatggcgg cggcgctgaa gcgggccggt atcacggctg ccgatgtcga ttacgtcaat
     2461 gcccatggca cctcgacgcc ggtgggcgac gagatcgaac tcggcgctgt ggcgcggctg
     2521 gtcggcaatt gcgccgccaa cctgtcgatg tcctcgacca agtcggcgat cgggcatctg
     2581 cttggcgccg ccggcgcggt tgaagccatc ttttctattc tggcgatccg cgaccagatt
     2641 gcgccgccga cgctcaatct cgacaatccg tctgtggaaa ctccgatcga tctggttccg
     2701 cacaaggccc ggccgcgcaa gattgacgtg gcgctgtcga attcgttcgg gtttggcggc
     2761 accaacgcgt cgctggtgtt ccggcggtat gcgtgacggc gacaggggct ctccctttcg
     2821 ccataattga acgataacgg tgccgctcag gcatgcggag acgtggtgga tggccgataa
     2881 ctccaacccc ccggaggaga agcagacgcg acggcgctcg ctgttcagcc cgcgcgacaa
     2941 gctgaagcct gtggctgccc cgccgccgcc gccgcgtccc aagcgcaagc agggcggcat
     3001 gatcgccgtc ctgtccgcgg tgctgaccgt cagcgtcatc attctggtgg ccgcgaccgc
     3061 cggtatcggc tacgtcaagc gcatggtcgc ctcacccggg ccattgcaga cggaaaaatc
     3121 cattgtcatc gagcgcggcg ccggcgccga ggaaatcgcc gaactgctgg agcgcgaagg
     3181 catcatcacc cagccgacgc tgttccaggt gctggcgatg tcgcaggtct atggccgcct
     3241 caaggcgggc gagttcctgt tcaagcgcga ggcaagcctg tccgatgtga tcgataccat
     3301 cgccaacggg cgggccatcg agcatacgat caccattccg gaagggctga ccagcgagca
     3361 gatcgtggcc aggctgaaag agagcgatct tctaaccggc gaggtggcca gcgtgccgcg
     3421 cgagggggcg ctgttgcccg acacctacag gatcgcgcgc ggcacttcgc gggattcgct
     3481 gcttgcccgc atggagaagg agcagaagcg cgttcttggt gaggtctgga acaagcgcgc
     3541 caaggacgtg ccgctgaaat cgcccagcga tctggtcatt ctcgcgtccg tggtcgagaa
     3601 ggaaaccggc aaggcggacg aacgcagccg ggttgcgggc gtttttgtca accggctcaa
     3661 tcgcaacatg aggctgcagt cggacccgac catcgtctac gggctggtcg gcggcaaggg
     3721 cacgctgggt cgcggcatca cccgcgccga catccagtcg aacacgccct acaataccta
     3781 tgtcatcaac ggcctgccgc cgggccccat cgccaatccc ggccgcgcct cgctggaagc
     3841 cgccgccaac ccctcgcgca cccgggaatt gttcttcgtg gccgatggta cgggcggcca
     3901 tgccttctcc gagacattgg aacagcatga gcgcaacgtg gcccgctggc ggcagatcga
     3961 ggcgcagcgc gatagcgggg cggcgccagg attcggggcg gtgccgctgg cgccgggggc
     4021 ggctccctc
//
LOCUS       DAOMXG010000197         3980 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_12330_length_3980_cov_9.723822, whole genome shotgun sequence.
ACCESSION   DAOMXG010000197 DAOMXG010000000
VERSION     DAOMXG010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3980
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12330_length_3980_cov_9.723822"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /gene="tolQ"
                     /locus_tag="PLQ11_11135"
     CDS             complement(<1..682)
                     /gene="tolQ"
                     /locus_tag="PLQ11_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003602810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HRK25495.1"
                     /translation="MSPVEPATVAELTLFGMFWNAHFVVKLVMFGLFAASIWTWAIII
                     DKMLMFRRFKTGMNRFEETFWSGQSLEELYRTLSQRPTSGMATLFVAAMREWKRAFEG
                     TGKGFNSLHGRIDKVLDVSIQREVERLESKLFILATVGSASPFIGLFGTVWGIMTSFR
                     SIAASKNTSLAVVAPGIAEALFATAIGLVAAIPAVIAYNKFQKDLNRSQSRLEGFAGE
                     ISAILSRQI"
     gene            complement(904..1323)
                     /gene="ybgC"
                     /locus_tag="PLQ11_11140"
     CDS             complement(904..1323)
                     /gene="ybgC"
                     /locus_tag="PLQ11_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HRK25496.1"
                     /translation="MTATHSLALRIYYEDTDFSGFVYHARYLHFFERARTEMLRDLGH
                     DQRAMFANSEGPEAFVVRRMNIDFLRPALMDDIVEVRSRTLKVQGATMQIDQRLFRGE
                     ELLCSALVQVAYLVGGKPRRMPESLRALFAGDAGEQD"
     gene            complement(1320..1673)
                     /locus_tag="PLQ11_11145"
     CDS             complement(1320..1673)
                     /locus_tag="PLQ11_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25497.1"
                     /translation="MPIRDVPRRLQAIVSLIDGMIEQTHGQAFDHIRGNFLYERAIER
                     VIELISEESETLAEVLGPRHPGAELQPVLALAEQLVDNDYRIETYDMWQLLTRQLPAL
                     KAAILPILTDYTFEI"
     gene            complement(1748..2407)
                     /locus_tag="PLQ11_11150"
     CDS             complement(1748..2407)
                     /locus_tag="PLQ11_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25498.1"
                     /translation="MKLFWRLYALGGALLFAYSVMGLVVSYTPSGTLLLMPGQVAETS
                     IFRLHEKMMQLTVRFPRDDVGKAQDSPEAIQRRKMAAAPMSLTVTRSDGVTRQYHAYR
                     QTGYDFNPPHFYRPFSSLKPEKVDAWLPTDFSQNLPLAPGINRISLKVETVDPVLEGL
                     KVHWIIPSPLGFKSSAPELKWLFWSLSWMIYLPAYALLGIVLALAGRIARSRRGETNV
                     T"
     gene            complement(2412..3452)
                     /gene="ruvB"
                     /locus_tag="PLQ11_11155"
     CDS             complement(2412..3452)
                     /gene="ruvB"
                     /locus_tag="PLQ11_11155"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HRK25499.1"
                     /translation="MTDTRLVTGQKRETDAFEATIRPQSLAEFVGQEQARQNLEIFIK
                     AARARGDALDHVLFAGPPGLGKTTLAQIMAKEMGVGFRATSGPVISKAGDLAALLTNL
                     EERDVLFIDEIHRLNPAVEEILYPAMEDYQLDLMIGEGPAARSVKIDLPKFTLVGATT
                     RQGLLTTPLRDRFGIPVRLNFYTVEELEKIVKRAARVMGIGIAEDGANEIARRARGTP
                     RISGRLLRRVRDFAIVEGAPSITRALADKALGYLDVDSIGLDQMDRKYLTMVAVNFGG
                     GPVGIETIAAALSEPRDAIEEIIEPYLIQQGFVQRTPRGRLLTDHAFRHLGLAAPQRA
                     PAQFGLFTDGEE"
     gene            complement(3550..>3980)
                     /locus_tag="PLQ11_11160"
     CDS             complement(3550..>3980)
                     /locus_tag="PLQ11_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25500.1"
                     /translation="RRALAVGAFAELAGPPVGVWLILIPMAVRNGVGADGPLGIVSAI
                     GGLTMMSYVFGGLPALVAAVVVGTITYRRGRYGYGHAVSAAVLGTLAMSALLAVGDAK
                     PADEFVSIAQGIAPLAVGSALICRWLTKRLGLLPGREAPA"
BASE COUNT          771 a         1269 c         1239 g          701 t
ORIGIN      
        1 cgatctggcg cgacaggatc gccgagattt cgccggcaaa gccttcgagc cgcgactggc
       61 tgcggttgag atccttctgg aacttgttgt aggcaatcac cgccggaatc gccgcgacca
      121 gaccgatagc ggtggcaaac agcgcctcgg cgatgccggg cgccaccacc gccagcgagg
      181 tgttcttcga tgctgcaatg gagcggaacg aggtcatgat gccccagacc gtgccgaaca
      241 ggccgatgaa cggcgaggcc gagccgaccg ttgccaggat gaacagcttg gattcgagcc
      301 gctccacctc gcgctggatc gacacatcca gcaccttgtc gatgcgacca tgcagcgaat
      361 tgaacccctt gccggtgcct tcaaacgccc gcttccattc gcgcatcgcc gccacgaaca
      421 atgtcgccat gccgctggtc ggccgctgcg acagcgtgcg gtagagttcc tcaaggctct
      481 ggccggacca gaaggtttcc tcgaagcggt tcattccggt cttgaaccgc cggaacatca
      541 gcatcttgtc aatgatgatt gcccaggtcc agatggatgc ggcaaacagg ccgaacatca
      601 ccagcttgac cacgaaatgg gcattccaga acatgccgaa cagggtcagt tcggcaacgg
      661 tggctggttc gactggactc atacgggatc tccctggcct gccggtccgg gcaggacatc
      721 ggcgccagat atccggcggg actgtggccg ggctttggcg cgggttttcg accaaactac
      781 ggcacaacga cggcaatccg ccgcagagca tcagatccgc agcatatccg atcaatccgc
      841 cctgaatggt taatgtttgg ttgagatggc tctgctttgc gcgcagggat gcctgcgcaa
      901 ggttcagtcc tgctccccgg catcgccggc aaacagcgcc cgcaggctct cgggcatgcg
      961 gcggggcttg ccgccaacca gataggcaac ctgcaccaag gccgaacata agagctcctc
     1021 gcccctgaac agccgctggt cgatctgcat ggtcgccccc tgcaccttga gtgtccgcga
     1081 gcgaacctcg acgatgtcat ccatcagcgc cgggcgaagg aagtcgatgt tcatgcgcct
     1141 gacgacgaag gcttccggcc cttcgctatt ggcgaacatc gcccgctggt catggccgag
     1201 atcgcgcagc atctcggtgc gcgcccgctc gaagaaatgc agatagcgcg cgtgatagac
     1261 gaaaccggaa aaatccgtgt cctcgtagta gatgcgcagg gccaggctgt gcgtcgctgt
     1321 catatctcga acgtgtaatc ggtcaggatc ggcaggatcg ccgccttcag cgccggcagc
     1381 tggcgggtca gcaattgcca catgtcgtag gtctcgatgc ggtaatcatt gtccaccagt
     1441 tgctccgcca gcgccagcac cggctgaagc tccgcacccg gatggcgcgg ccccagcact
     1501 tcggccagcg tttcggattc ttcagatatg agttcgatca cccgttcgat ggcacgctcg
     1561 tagaggaagt tgccgcggat atgatcgaac gcctgcccat gcgtctgttc gatcatcccg
     1621 tcgatcaggg acacaatggc ttgcagccgg cgcggcacgt cgcggattgg catggtggat
     1681 ctactcgtca gattctgtga acggggccaa ttgagccagc ttgcagtcgg ctcggcaaga
     1741 tcggggctca ggtgacattg gtctcgcccc gcctgctgcg cgcgatacgg ccagccagcg
     1801 ccagaactat tccaagcagc gcataggccg gcaggtagat catccacgac aggctccaga
     1861 acagccactt gagttccggg gcactgctct tgaaacccag cggggaggga atgatccagt
     1921 gcaccttgag cccttcaagt accggatcca cagtctcgac cttgagggaa atgcgattga
     1981 taccaggagc cagtggcaag ttctgactga aatcggtcgg caaccatgca tccacctttt
     2041 ccggcttcag actcgaaaaa ggtcggtaaa aatggggagg attaaagtca tatccggttt
     2101 ggcgataggc atgatactgg cgggtgacgc cgtcggaacg cgtaacggtc aggctcatgg
     2161 gtgcagccgc catcttcctg cgttggatcg cctccggact atcctgagcc ttgccgacat
     2221 catcgcgcgg aaatctcacg gtgagctgca tcattttctc atggagccga aaaatcgacg
     2281 tctcggcgac ctgtccgggc atcagcagca acgttccgga tggagtgtaa ctcacgacaa
     2341 gtcccatgac cgaataggca aacagcagcg ccccgccaag agcatacaat cgccaaaaca
     2401 atttcatgaa actattcttc cccgtccgta aacagcccga actgcgccgg ggctcgttgc
     2461 ggcgcggcaa ggccgaggtg acggaaggcg tggtcggtca gcagccgtcc gcgcggggtg
     2521 cgctgcacaa aaccctgctg gatgagataa ggttcgatga tttcctcgat ggcgtcgcgc
     2581 ggttcggaga gtgcggcggc gatcgtctcg atccccaccg gaccgccgcc gaaattgacc
     2641 gcgaccatgg tcagatactt tcgatccatc tgatcgagac cgatcgaatc aacatcgaga
     2701 tagcccagcg ccttgtcggc cagcgcccgc gtgatcgacg gcgccccctc gacgatggca
     2761 aagtcgcgca cacggcgcag cagccggccg gagatgcgcg gcgtcccgcg ggcccggcgg
     2821 gcgatttcgt tggcgccatc ctcggcaatg ccgatgccca tcacccgcgc ggcgcgtttg
     2881 acaatctttt ccagttcctc gaccgtgtag aaattcagcc gcaccggaat gccgaaccgg
     2941 tcgcgcaaag gggtcgtcag cagcccctgc cgggtcgttg cgccgaccag cgtgaacttc
     3001 ggcagatcga tcttgaccga acgcgccgcc ggcccctcgc cgatcatcag gtcaagctga
     3061 taatcctcca tcgccggata gaggatctcc tccacggccg gattgaggcg atggatctcg
     3121 tcgatgaaga ggacgtcgcg ctcctcgagg ttggtcagca gggccgcgag atcgcccgcc
     3181 ttcgagatca cggggccgga ggtcgcgcgg aagccgacac ccatctcctt ggccatgatc
     3241 tgcgcgagcg tcgtcttgcc gagacccggc gggcccgcga acaggacgtg gtcgagcgca
     3301 tcgccgcgcg cccgcgccgc cttgatgaag atctcgaggt tctgccgcgc ctgctcctgc
     3361 ccgacgaact cggcgagcga ctgcggacgg atggtcgcct cgaaggcgtc cgtctctcgc
     3421 ttctggccgg tgacgaggcg ggtgtcggtc ataggttcgc ttgcccgatg atcctgtggg
     3481 tgtgccggag tcgggttgat ttggcaaccg cgctcgttct ctttttgtct gtcgtggccc
     3541 gctgcgcggt caggccggag cttcccgccc gggaagtagg ccgagccgct tcgtcagcca
     3601 ccggcagatc aaggccgagc cgacggccag cggcgcgatg ccctgggcga tggagacgaa
     3661 ttcgtccgcg ggcttggcat caccgacggc aagcagcgcg gacatggcga gcgtcccgag
     3721 cacggcggcc gacaccgcat gaccgtagcc gtagcggccg cgccgatagg tgatcgtgcc
     3781 gaccacgacg gccgcaacga gcgccggcaa gccgccgaag acgtacgaca tcatggtgag
     3841 accgccgatg gcggagacga tgccgagcgg tccgtcggcc ccgacgccgt tcctcaccgc
     3901 catcggtatc aggatgagcc agacgccgac cggcgggccc gcgagctcgg cgaacgcgcc
     3961 gacggccaag gcgcgccgcc
//
LOCUS       DAOMXG010000198         3955 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_12441_length_3955_cov_7.881282, whole genome shotgun sequence.
ACCESSION   DAOMXG010000198 DAOMXG010000000
VERSION     DAOMXG010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12441_length_3955_cov_7.881282"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="PLQ11_11165"
     CDS             <1..546
                     /locus_tag="PLQ11_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25501.1"
                     /translation="GLSAAMAEPAEPPVEVLTPVDEPESPAAPAQPDDPLQAFEAELD
                     KLIPLKPGRKAKARKDKAEAVEAPIAPVPPVPSVPAPAPVVVAEMASVPGEPEPVPEP
                     PARPSRPVFAPVVEDDPFLPAPEAPREGAEVVGAYESGGAKYTMFSDGSVIAEADGQK
                     LFFRTLDDLRAFIDGALRDGS"
     gene            complement(580..1011)
                     /gene="msrB"
                     /locus_tag="PLQ11_11170"
     CDS             complement(580..1011)
                     /gene="msrB"
                     /locus_tag="PLQ11_11170"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012707327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HRK25502.1"
                     /translation="MTKRPDRPFSDAEWRARLTDEQYRVTRQHGTERPFTGPYLNEKR
                     PGTYRCICCDAPLFASEAKYESGSGWPSYFAPIDNQAISEHPDNSLFMTRTEIRCAHC
                     AAHLGHVFPDGPRPTGLRYCVNGTALRLHARDKSDTNDAPD"
     gene            1391..1798
                     /locus_tag="PLQ11_11175"
     CDS             1391..1798
                     /locus_tag="PLQ11_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HRK25503.1"
                     /translation="MSDETSYIELAAEIVSAYVSNNSVPSSELPALIADIHGALKRLN
                     GTVVEEPSEPQKPAVSVKKSIAADFIICLEDGKKFKSLKRHLRTQYNMSPEQYREKWG
                     LPADYPMVAPNYAAARSRLAKEMGLGQQRRRGR"
     gene            2263..3123
                     /locus_tag="PLQ11_11180"
     CDS             2263..3123
                     /locus_tag="PLQ11_11180"
                     /inference="COORDINATES: protein motif:HMM:NF040428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6456 domain-containing protein"
                     /protein_id="HRK25504.1"
                     /translation="MAPVAPTPSHPGQADAPISAARSGSDLGAEARMLLALLDRPGAE
                     GRVREGRLVVYARRSGVTLALGSVAACVVERLLQAGMIVTDRDGAHQRYRLAPGGGAV
                     RPEADTDGVPDDAEAFAAPLRNPAESPLAWLYRRGGWIGPAEFAAGERLHADLACACL
                     LPRTTADWNRLAAAAPGAGLNPTEAMLAARQRVSRALGVVGSDFSGLLVDVCGFLKGL
                     DQIEQERRWPARSAKVVLGMALTRLARHYGLSNSAEGSKGSRRVHRWSAERGRPAFVT
                     EAGLSDQPSD"
     gene            complement(3213..3644)
                     /locus_tag="PLQ11_11185"
     CDS             complement(3213..3644)
                     /locus_tag="PLQ11_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HRK25505.1"
                     /translation="MSTDLDSIIDDLAFLDDWEERYRYIIDLGRTLAALNADERSEQN
                     KVRGCASQVWLVSEVQPSPEGPRLRFRGDSDAHIVKGLVALTLALFSGRTAQDILATD
                     AQALFARIGLKEHLTPQRSNGLAAMVERIRADARHTIGHEK"
BASE COUNT          690 a         1205 c         1297 g          763 t
ORIGIN      
        1 gggctttccg ccgcgatggc ggagccggcc gagccgccgg tcgaagtgct gaccccggtc
       61 gacgagccgg aatcgcccgc tgcgccggcg cagcctgacg atccgttgca agcattcgag
      121 gctgagctgg acaagctcat tccgctcaaa cccggcagaa aggccaaggc ccgcaaggac
      181 aaggccgagg ccgtcgaagc ccccattgcg ccggtccctc ccgtcccatc cgtgccggca
      241 cccgccccgg tcgtggtggc tgaaatggcg tcagtgcctg gagagcccga gccagtgccc
      301 gagccgcccg cccggccatc gcggccggtt tttgcccccg ttgtcgagga tgatcctttc
      361 ctgccagcgc ccgaggctcc gcgcgaaggt gcggaggtcg tcggcgccta tgaatcgggc
      421 ggagcgaaat acacgatgtt ctccgatggc tcggtaattg ccgaggccga tgggcagaag
      481 ctgtttttcc gcacgctgga cgatctgcgc gccttcattg atggcgcctt gcgggacggg
      541 agctgaacgc tgcccttgcc cctgccgttg cccggcgggt cagtccggag cgtcattggt
      601 gtcgctcttg tccctggcat gcagcctcag cgccgtgccg ttcacgcaat agcgcaatcc
      661 ggtcggccga gggccatcgg gaaagacatg gccgagatgg gcggcgcaat gggcgcagcg
      721 gatctcggtc cgggtcatga acagcgaatt gtccggatgt tcgctgatcg cctgattatc
      781 gatcggggcg aagtaactcg gccagccgga gccggactca tatttggctt cggacgcgaa
      841 caacggcgca tcgcaacaga tgcagcgata ggtgccgggg cgcttctcgt tgagataggg
      901 cccggtgaag gggcgttccg tgccgtgctg gcgggtgacg cgatattgct cgtcggtcag
      961 gcgcgcacgc cattcggcat cgctgaacgg gcgatcgggg cgtttggtca tgacttgctc
     1021 cataggcatt ggctgcgctg gaaagataat gcgcgctgga ttgccgattc aatgtcctgc
     1081 aagccaaggt gccggacttg tgatggccgg gcttgctttt gcatccttgg aaacgtcact
     1141 tgtcggcatg caatgccccg aaacggaagt tggaacaaga atacaagatt caatattgct
     1201 tccaaatact atctgatcta tgtctatggc aattcatgga taggaatcgg ccaagtcact
     1261 agtttggtcg acgggggcaa accatttttc tctccatgaa ggggatctgc gcgacgagat
     1321 tgggcaataa cgtgccgtcg ctcaggtttt gggcaatccg ttcgcatgtt ctcacaacaa
     1381 ggacaacaag atgtcagatg aaacaagtta tatcgaattg gccgctgaaa tagtctctgc
     1441 ctatgtcagc aacaattcgg ttccctcttc ggaactgccg gcgctgattg ccgatatcca
     1501 tggcgctctc aagcgcctca atggcaccgt tgtcgaggag ccgagcgagc cgcagaagcc
     1561 ggccgtttcg gtcaagaagt caatcgcggc tgatttcatc atctgccttg aagatggcaa
     1621 gaagttcaag tcgctcaagc gccatctgcg cacccaatac aacatgtcgc ctgagcagta
     1681 tcgcgagaag tggggccttc cggctgacta cccgatggtg gcgcccaact atgcggccgc
     1741 ccgctcgcgt ctcgccaagg agatgggcct gggtcagcaa cgccgtcgcg ggcgctgatc
     1801 gcagcggtcc ctgtccgggt tcgattggcg ccgcccgaag gggcggcgct tttcgtttcc
     1861 ggggctggtg cctctggctg gtctgccgat gccaagcact cggccgtcag cgcctgcgcc
     1921 agccgggcat gccttgccag atcatagctc cagtgccctg attgccccag tcggcgctcg
     1981 cgcagcagtg cgccttgcag ataagcaagg attttgaacc tatccggcat gccataggca
     2041 aacagcgcgg gcaacagccg cgctgcatcg cgcgaccgat catagccgtg tgccccgcgc
     2101 tgaacgaggc tgtccgacca caggccggcg cgttcttcga ggtcttttgc agcaatgccc
     2161 aattttggct tcgtcatctg tcatccagcg tcaagggata atagtcttga cataagaaaa
     2221 taatccttat tctctgtcaa gggtcagcat gaggagagtt ccatggcccc tgtcgcccct
     2281 acgccaagcc accccggaca agcggatgcg ccgatcagcg cggcccgatc aggcagcgat
     2341 ctgggagcag aagcgcgcat gctgctggcg cttctcgatc ggccgggcgc ggaagggcgg
     2401 gtccgggagg ggcggcttgt cgtttatgcg cgccggtcgg gcgtgacgct tgcgctcgga
     2461 tcggtggctg cctgtgtcgt agagcgcctg ttgcaggccg gaatgatcgt caccgaccgt
     2521 gacggtgcgc atcagcgcta ccggttggcg cctggcggcg gagccgtgcg gccggaggca
     2581 gacacggacg gagtgccgga cgatgcagag gcgtttgcag cccccctgcg caatccggcc
     2641 gaaagtccgc ttgcatggct gtatcggcgc ggcggctgga tcggtccggc cgaatttgcc
     2701 gccggcgagc ggttgcatgc cgatctggcc tgcgcctgcc tgttgccgcg cacgacggcg
     2761 gactggaacc ggttggctgc ggctgctccg ggcgccgggc tgaacccgac cgaggccatg
     2821 ctggctgcac gccagcgcgt atcccgcgct ttgggcgtgg ttgggtcgga tttttccggg
     2881 ctgctggttg atgtctgcgg atttctcaag gggctcgacc agatcgagca ggagcggcgc
     2941 tggccggccc ggtcggcgaa ggtcgtgctg gggatggcgc tgacccggct ggcccgtcac
     3001 tatggtctgt cgaacagcgc ggaaggcagc aaagggtcgc ggcgcgtcca tcgctggagc
     3061 gccgagcgcg gccgccccgc cttcgtcacc gaggctggcc tgtccgacca gccgagcgac
     3121 tgataccagg ggtcatggca actcaccctt ggttcctgct tgaattctca ttatttttca
     3181 atgggaaatg aaaattcaaa ttacgtcatc ggtcacttct cgtgaccgat ggtgtggcgg
     3241 gcatctgcgc ggatccgctc gaccatcgcc gccagaccgt tcgagcgttg cggggtgagg
     3301 tgctccttga ggccgatgcg ggcgaacagc gcctgggcgt cggtggcgag gatatcctgc
     3361 gctgtgcggc cggagaacag ggccagcgtc agggccacga gccccttgac gatatgggca
     3421 tcgctgtcgc cgcgaaagcg caggcgcggt ccttccggcg agggctgcac ctcgctgacc
     3481 agccagacct ggctggcgca gccgcgcacc ttgttctgtt cactgcgctc gtccgcattc
     3541 aatgcggcaa gtgtccggcc cagatcgatg atgtagcggt agcgctcttc ccaatcgtcc
     3601 agaaaggcaa gatcgtcgat gatactgtcg aggtcggtgc tcatggtctc gtcatgcgcc
     3661 aagcccgccc gcttgtccac atccttgacc atgcggcgaa agagcggttg cgcaaggcgg
     3721 ccgaagcatt gccgtaatat attgaaaaac aaatcctatt ccgtgagaca gcgggcgagc
     3781 caggcgcccc gctttgtcat ggacgtggcg accgtccaga cggataagga cccttcggcg
     3841 ggtccgccgt catgcggtcg aggctttcgc gtgcagccgc ccggcagatg tccggctggt
     3901 gctggcacag ggccagggcc gatcgcgcca tcgtgccggc atcggcaacc cccgg
//
LOCUS       DAOMXG010000199         3934 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_12519_length_3934_cov_10.557618, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000199 DAOMXG010000000
VERSION     DAOMXG010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12519_length_3934_cov_10.557618"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="PLQ11_11190"
     CDS             <1..440
                     /locus_tag="PLQ11_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012453345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRK25506.1"
                     /translation="IAAGLARDGHVYILDDATLDGARPDVWAARALATYHRHEADALI
                     AEINQGGAMVAEVLRSIDASVPVTTVRATRGKLLRAEPVAALYAQGRVHHLGAFPALE
                     DEMANFGPDGLSQGRSPDRLDALVWAVTALALDREGSPRLRRL"
     gene            complement(454..2457)
                     /locus_tag="PLQ11_11195"
     CDS             complement(454..2457)
                     /locus_tag="PLQ11_11195"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRK25507.1"
                     /translation="MLSSFQKFFAGLRLDATSEEMAREQYKTLTRTIPFLYTVCIGTC
                     IALMATFSRAAPAWLAYWIPGSVAAIMLVRRQFWLKQRGTEYSADIASIRRNMLGTAI
                     LGPVFTFAFSVTGVLLMGYANPYQESLCVVAIWITAIASAFCLSVLPIASVTVVISSA
                     IPLIVAFVLSGSTQMIIIAGIFAVVSAMMIYILLDSFNRFAEILNSRTLIDERQRQTE
                     AARQAVTEMAYTDTLTNLPNRRAFERLLSERVAMTTSGVLRCFTVGMVDLDGFKPIND
                     AYGHVVGDEVLVQASRRLMGAMKGFGAIARVGGDEFAIIVDNIEGEAEISDLAQRLTS
                     AFSDPIIVGSITTRLACSCGFAPFPGSGQETSRLIDRADMALYRAKAERRGSFCIFSA
                     SLEKAALERASIEQELRLAVAADALTVHFQPIVDLKTGRLAGFEALARWTHPELGNVS
                     PAIFVPIAEQTGLIETMTDRLLFKAARIAAQWPADLMLAFNLSAYQVGKPNAGLRIIR
                     MLAEAGLPPHRLEAEITETAILQNIEASRQTIETLKAAGVRISLDDFGTGFSSLSHVK
                     DLPLDKIKIDKSFVDRVCHDDKIGNIVRSIINMCEFLDLACVAEGIEHSEQLDFLKDN
                     KCSGGQGYLFSRPIPTDRVDTLLKDIAARRTKRESSPAAAQAA"
     gene            2798..>3934
                     /locus_tag="PLQ11_11200"
     CDS             2798..>3934
                     /locus_tag="PLQ11_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009339028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HRK25508.1"
                     /translation="MKHLLKRFLPRAEDTKVSRVAPMIALAGIGRPLWTERSSAELTG
                     LGYMKNAIAYRCIRMIVEGAASLRYDVFRDSEELENHPLRSLLLTPNPRQTGADLFEA
                     LYVNLLCFGNAYLELVADGRTPRELYALRPDRVRVVPGADGWPEAYDYAVGGQSVRLR
                     MDGAALPPVLHLRMFDPLDDYYGMAPLAAAQVPLDIHATASAWNKALLDNSARPSGAL
                     VYATDGANMTDQQFARLKEELETTFSGALNAGRPILLEGGLDWKPLALSPKDMDFTEA
                     KSAAAREIALALGVPPLLLGLPGDNTFANYAEANRALWRQTILPLAQRTMQALAHWLA
                     PAYGAGLRLELDLDKVEALAPERDALWKRLEGATFVERDEKRDLA"
BASE COUNT          735 a         1222 c         1272 g          705 t
ORIGIN      
        1 tcattgcggc cggactggcg cgcgacgggc acgtctacat cctggatgac gcaacgctcg
       61 atggcgcccg gccggatgtc tgggccgcac gggcgctggc gacctaccat cggcatgaag
      121 ccgacgctct gatcgccgag atcaatcagg gtggcgcgat ggtggccgaa gtgctgcgat
      181 ccatcgatgc cagcgttccg gtgacaacgg ttcgggcgac gcgcggcaag ttgctgcggg
      241 cagagccggt cgccgcgctc tatgcgcagg gccgggtgca ccatttgggc gcctttccgg
      301 cgctggagga tgaaatggca aacttcggtc ctgacggact gtcgcaggga cgctctcccg
      361 atcggctcga tgcgctggtc tgggcggtga cggcgctcgc gctcgatcgg gaggggtctc
      421 cacggctcag gcggctgtga tctctcctcc ttgtcaggcc gcctgtgctg ctgcagggct
      481 ggattcacgc ttggtccgcc gcgcggcgat gtccttgagc agggtatcga ccctgtcggt
      541 cgggattggc cgcgagaaca ggtatccctg cccgccggaa catttgttgt ccttgaggaa
      601 gtcgagctgt tcggaatgct cgatcccctc ggcgacgcag gcaaggtcga ggaactcgca
      661 catgttgatg atcgagcgga cgatattgcc gatcttgtcg tcgtggcaga cgcgatcgac
      721 aaagctcttg tcgatcttga tcttgtcaag cggcaggtcc ttgacgtggc tgagcgagga
      781 gaacccggtg ccgaaatcat cgagcgagat gcgcacgcct gccgccttca gcgtctcgat
      841 cgtttgccgc gaggcttcga tgttctgcag gatcgccgtc tcggtgatct cggcttcaag
      901 gcggtgcggc ggcaggccgg cttcggccag catgcggatg atcctgagcc ccgcattcgg
      961 cttgccgacc tgataagccg acaggttgaa cgccagcatc agatcggccg gccattgggc
     1021 agcgatgcgt gctgccttga acagcagccg gtcggtcatc gtctcgatca ggccagtctg
     1081 ttcggcgatc ggcacgaaga tggccggcga aacattgccg agttccggat gggtccagcg
     1141 ggccagcgcc tcgaaaccgg caaggcgacc ggttttcaga tcaacgattg gctggaaatg
     1201 caccgtcagt gcatctgcgg cgacggccag gcgcaattcc tgctcgatcg aggcgcgctc
     1261 cagcgccgcc ttttcgaggc tggcggaaaa gatgcagaaa ctgccgcggc gttcggcctt
     1321 ggcgcgatac agcgccatgt cggcgcggtc gatcaggcgg ctggtctcct gacccgagcc
     1381 gggaaacggc gcaaagccgc aggaacaggc cagacgcgtg gtgatcgaac cgacgatgat
     1441 cggatcgctg aatgccgatg tcaggcgctg ggcaaggtcg ctgatttcgg cctcgccctc
     1501 gatattgtcg acgataatgg caaactcgtc gccgccgaca cgggcgatgg cgccgaaccc
     1561 tttcatcgcg cccatcagcc ggcgcgacgc ctgcaccaga acctcgtcgc cgacgacatg
     1621 gccataggca tcgttgatgg gcttgaaacc gtcaagatcg accatgccga cggtaaagca
     1681 gcgcaacacg ccgctggtgg tcatcgccac gcgctcgctg agcagccgct cgaacgcccg
     1741 acggttcggt aggttggtga gtgtgtcggt gtaggccatc tccgtcacgg cctgacgggc
     1801 ggcttcggtc tggcgctggc gctcatcgat gagggtgcgg gaattgagga tttcggcaaa
     1861 gcggttgaag ctgtcgagca ggatgtagat catcattgcc gagacaacgg caaaaatgcc
     1921 cgcgatgatg atcatctggg tcgagccgct caacacgaag gccacgatca gcggaattgc
     1981 cgagctgatg acgacggtga cggaggcaat cggcagcacc gagaggcaga aggcgctggc
     2041 aatcgcggtg atccagatgg ccacgacgca caaggactcc tgatagggat tggcataacc
     2101 catcaggagg acgccggtga ccgaaaaggc aaaggtgaac accgggccaa ggatggcggt
     2161 gcccagcatg ttgcggcgga tgctggcaat atcggcggaa tattcggtgc cgcgctgctt
     2221 cagccagaac tggcgccgca ccagcatgat cgcggcgacg ctgccgggaa tccagtaggc
     2281 cagccaggcc ggcgccgccc tggagaacgt cgccatcagg gcgatgcatg tgccgatgca
     2341 cactgtgtag aggaacggga tcgtccgagt cagcgtcttg tattgctcgc gtgccatctc
     2401 ttcggaggtc gcatcgagac gcaggccggc aaagaatttc tgaaaagacg ataacattcg
     2461 gatgcacacc ccattgaatg tgcggaaaca tatgccgccg cgtcagtaga agccaggtaa
     2521 cggcattggt tagaatttga atggattggg ccaccccgtg gatcttgctg aagtatctat
     2581 taagtcgtta tccgaaaacc agcctctcac caaagcttgc ccttttgccg ccgtcggcct
     2641 ggccacgaca gtgcctggag catggcgcga tcaagcagga accggtcagt cgcgttagcc
     2701 atgctccaag ttattgattt cgatcacttt atccaatttc tggcgattcc accaaaaatc
     2761 ggcgtgatcg agccgattgc cccgatgcga gagactcatg aaacatctgc tgaaacgctt
     2821 cctgccaaga gccgaggata ccaaggtctc gcgcgtcgcc ccgatgatcg cgctcgccgg
     2881 catcggccgt ccgctgtgga cggaacggtc gtcggccgag ctgactgggc ttggctacat
     2941 gaagaatgcc atcgcctatc gctgcatccg catgatcgtc gagggggcgg cgagcctgcg
     3001 ctacgatgtg ttccgcgaca gcgaggaact cgaaaaccac cccctgcgca gcctgctgct
     3061 gacgccgaac ccgcgtcaga ccggggccga tctgttcgag gcgctctatg tgaacctgct
     3121 gtgcttcggc aacgcctacc tcgaactcgt tgccgatggc cgcaccccgc gtgaactcta
     3181 tgcgctgaga cccgatcgcg tccgcgtcgt gccgggtgct gatggctggc cggaagccta
     3241 tgactacgcg gtcggcggcc agtcggtgcg gctgaggatg gacggtgccg cattgccgcc
     3301 ggtgctgcat ctcaggatgt tcgatccgct cgacgattac tacggcatgg ccccgctcgc
     3361 cgccgcgcag gtgccgctcg acatccacgc caccgccagc gcctggaaca aggcgctgct
     3421 cgacaactcc gcccgcccgt ccggcgcgct ggtctatgcc accgacggcg ccaacatgac
     3481 ggatcagcaa ttcgcccggc tgaaggagga gcttgaaacc accttctccg gcgcgctcaa
     3541 cgccggccgg ccgatcctgc tcgaaggcgg gctcgactgg aagccgctcg cgctcagccc
     3601 caaggatatg gatttcaccg aggccaaatc cgctgccgcg cgcgagatcg ccctggcgct
     3661 cggcgtgccg ccgctgctgc tcggcctgcc gggcgacaac acctttgcca attacgccga
     3721 ggccaaccgt gccctgtggc gccagacaat cctgccgctg gcgcagcgga cgatgcaggc
     3781 gctcgcccat tggctcgccc cggcctatgg cgccggattg cggctcgaac tcgacctcga
     3841 caaggtcgaa gcgctcgccc ccgagcgcga tgccctgtgg aagcggctgg aaggcgccac
     3901 cttcgtcgaa cgcgacgaaa agcgcgacct tgcc
//
LOCUS       DAOMXG010000200         3923 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_12568_length_3923_cov_9.199845, whole genome shotgun sequence.
ACCESSION   DAOMXG010000200 DAOMXG010000000
VERSION     DAOMXG010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12568_length_3923_cov_9.199845"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..113)
                     /locus_tag="PLQ11_11205"
     CDS             complement(<1..113)
                     /locus_tag="PLQ11_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012255506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HRK25509.1"
                     /translation="MFPDLTRDDVFRIETARLWLRWPRLADAAAIARQAGE"
     gene            complement(271..543)
                     /gene="rpmA"
                     /locus_tag="PLQ11_11210"
     CDS             complement(271..543)
                     /gene="rpmA"
                     /locus_tag="PLQ11_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRK25510.1"
                     /translation="MAHKKAGGSSRNGRDSHSKRLGLKKFGGENVLAGNIIARQRGTK
                     WHPGVNVGLGVDHTLFALVDGQVAFVQKGNGRTYVNVAPAPKAAAE"
     gene            complement(643..1260)
                     /gene="rplU"
                     /locus_tag="PLQ11_11215"
     CDS             complement(643..1260)
                     /gene="rplU"
                     /locus_tag="PLQ11_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HRK25511.1"
                     /translation="MFAVIKTGGKQYRVAANDVITIERLAGEAGDTITFGEVLMIGGA
                     TALIGAPTVAGASVVGELVEQARGPKVISFHKRRRKNSKRKRGHRQDLSVVRITDILT
                     DGKKAAAKAAKPAAKAEAPKHDPSDISLIGGIGPTLEKKLRAAGVTSWAQIAAWTEAD
                     IAKFDEELGLRGRVTREEWVEQAKELMAGKPPRAKVDQEAAKADK"
     gene            complement(1462..1758)
                     /gene="rpmB"
                     /locus_tag="PLQ11_11220"
     CDS             complement(1462..1758)
                     /gene="rpmB"
                     /locus_tag="PLQ11_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003613454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HRK25512.1"
                     /translation="MSRRCELTGKGVLSGHKVSHSNRKTKRKFLPNLANVTLLSDALG
                     KAVKLRVSTNALRTVEHRGGLDAFLVDAKDCELSRDALALKKALVKKNAEKASA"
     gene            2030..2857
                     /locus_tag="PLQ11_11225"
     CDS             2030..2857
                     /locus_tag="PLQ11_11225"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HRK25513.1"
                     /translation="MVVKMLKSSSGRLLAAGLLLCSGVSARAGSVDVQYAVSLAGLTL
                     GTATLSGSISPTRYSLNAQAHLTGLAGMVTGGKGAAAATGQFAAGQVVPGNYSLTASN
                     SEITRTVQMGLTSGNVDKVVVNPPLDPRPDRVPVTGSHQRGVLDPLGAALLSSPAKTP
                     ESACNRTLPVFDGAQRFDIQLSYAGTRNAAAKDGYSGPVVVCKARYTPIAGHRAERAA
                     VKYMANNRDMEAWLAPSGIEGVYLPFRVSIKTMIGTTVIQAVKYHIEPSETTAGLRR"
     gene            3159..>3923
                     /locus_tag="PLQ11_11230"
     CDS             3159..>3923
                     /locus_tag="PLQ11_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019904533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HRK25514.1"
                     /translation="MSKGWLSRLGVRSRLARLAGSAVLAAGLLGPSGTQTAIANGDTR
                     SLTLYHSHTQENITVTFRRDGSYDRSALEKLNWFLRDWRNDDATQMDPRLFDVVWATW
                     REVGSGAAIRIVSAYRSPGTNAMLRRRSSGVAKNSQHIQGKAMDLHMPDVSMAKVREI
                     GMRLQRGGVGYYPTAGTPFVHLDVGSVRSWPRMPRNQLERLFPDGKTVHIPADGTPLA
                     GYEAALAEIQTRGGSALSYAEITSPRTAGSASAASTA"
BASE COUNT          656 a         1290 c         1218 g          759 t
ORIGIN      
        1 ttctccccgg cctgacgggc gatggcagcg gcgtcggcaa ggcgcggcca gcgcagccac
       61 agccgtgcgg tttcgatgcg gaagacatcg tcgcgggtca gatcggggaa catcatcttt
      121 ctccagccca tgaatgcgca aaggggaagt ggtgaaccac ttcccctttg gtttttcggg
      181 tttccggaga tttgggtctc ttgacccagt tgctcctgaa gcctgccgcc gggggtggtc
      241 accggcgggg ctcctctgct tgccctgccg ttattcggct gccgctttcg gcgccggcgc
      301 gacgttgacg taggttcggc cgttgccctt ctggacaaag gcaacctggc catcgaccag
      361 cgcaaacagc gtatggtcaa cgccgagacc gacgttgacg cccggatgcc acttggtgcc
      421 gcgttggcgc gcgatgatat tgccggcgag cacgttttcc ccgccgaact tcttcaggcc
      481 gagacgcttc gaatgggagt cgcgcccgtt acgggacgaa ccgcctgcct ttttgtgagc
      541 catgatatgc tcctgaacct tctggccggc cttataccgg caaattcccg atcacgaaag
      601 ccctgaacaa cgggccttcc ggcttccttg acgtcagccg agttacttgt ctgccttggc
      661 cgcttcctgg tcgaccttgg cgcgcggcgg cttgccggcc atcagctcct tggcctgctc
      721 gacccattcc tcgcgggtga cacggccacg cagccccagc tcctcgtcga actttgcgat
      781 atccgcctcg gtccaggccg cgatctgcgc ccaggacgta acgccggcgg cccgcagctt
      841 cttttccagt gtcgggccga taccgccgat cagcgaaata tcgctgggat catgcttggg
      901 cgcctcagcc ttggccgcag gcttggccgc cttcgccgcc gccttcttgc cgtcggtcag
      961 gatatcggtg atgcggacga ccgacagatc ctgccgatgg ccgcgcttgc gcttggaatt
     1021 cttgcggcgg cgcttgtgga acgagatcac cttggggccg cgcgcctgct cgaccagttc
     1081 gccgacgaca gaggcgccgg caacggtcgg cgcgccgatc agcgcggttg ccccgccgat
     1141 catcagcacc tcgccgaagg tgatggtgtc gccagcctct ccggccaggc gttcgatggt
     1201 aatcacatca ttggcggcaa cacgatactg ctttccgccg gtcttgatca ctgcaaacat
     1261 ggtcgcttcg ctttcgtaag tccgtcatcg gagcggcaaa ccggtccgtg ccgtcttctt
     1321 gtggttcttt tggaatgtcg atcccgccgg ccgaaagctg acgaaaccag ccggtccgat
     1381 acagccaaag ctccggaccg tcaatgcgaa aacgccggcg gccgcggctg ccggcgatca
     1441 gatccgcagc gcggcaatcg ctcaggccga agccttttcc gcattcttct tgaccagcgc
     1501 cttcttcagg gccagcgcat cgcgcgacaa ttcgcaatcc tttgcatcaa ccaggaaggc
     1561 atccagcccg ccgcgatgtt ctaccgtgcg cagcgcattg gtcgacacac gcagcttgac
     1621 cgccttgccc agcgcatccg acaacagcgt gacattggcc aggttcggca ggaacttgcg
     1681 cttggtcttg cggttggagt ggctgacctt gtggccggac agcacaccct tgccggtgag
     1741 ttcgcaacga cgcgacataa gcgaacgcct ttcttgtcaa atcgacctgg atcgccgaca
     1801 gggtgcatca cccgcccgcg cggatctctc ggccagataa ttccggaacg aagccggggc
     1861 tatagggtct cgggctgtga aggtcaagct ggaaatcctt gccggctcgc ctggagagcg
     1921 ccatacattt caagaatgcg ccatacattt atggttaacc gttgcttgct taggatagcg
     1981 cattgaagcg gcccttgcct gggccaggac agcgttggga atcgtggtga tggtcgtcaa
     2041 gatgctgaaa agctcaagcg gcaggctgct ggcggccggt ctgctcctct gttcgggcgt
     2101 ttcggcccgt gccggctcgg tcgatgtgca atatgccgtt tcgctggccg ggttgacgct
     2161 tgggactgcg accttgtcag gctcgatttc gcctacccgc tattccctca atgcccaggc
     2221 ccatctcacc ggccttgccg gcatggtgac ggggggcaag ggcgccgccg ccgcgaccgg
     2281 tcagttcgcg gccggacagg ttgtacccgg caattattcg ctgacggcct ccaacagcga
     2341 gatcacccgc accgtccaga tgggcctgac ctccggaaat gtcgacaagg tggtggtcaa
     2401 tcctccgctc gatcccaggc cggatcgtgt cccggtgacc ggaagccatc agcgcggcgt
     2461 gctcgacccg ctcggcgcgg ccctgctttc aagcccggcc aagaccccgg agagcgcctg
     2521 caaccggacg ttgccggttt tcgacggcgc ccagcgattc gacattcagc tgagctatgc
     2581 gggcacccgc aacgccgccg ccaaggatgg ctattcgggg ccggtcgtgg tttgcaaggc
     2641 ccgctacacg cccatcgccg gccaccgtgc cgagcgcgcg gcggtcaaat acatggccaa
     2701 caaccgcgac atggaggcct ggctggcacc cagcgggatc gagggcgttt acctgccttt
     2761 ccgcgtctcg atcaagacga tgatcggcac gactgtcatt caggcggtca aataccacat
     2821 cgaaccgtcc gagacgaccg caggcctccg acgctgaggt cggacgatgg caccaaacca
     2881 ggccaaaggc cttgcgcgat gccgttatgg tgcagcctgt gcggcaagga ttgatccgcg
     2941 ccaatgcggc cgaagcgcga tccggtcaaa ttgcactgcg agagtggtca ggcttggttt
     3001 tgccatcatc tgcgggcaga gacgcctgcc tggcagtcgg gcggccggtt cctgcgtggg
     3061 aggaggcatt ttttgatgct atcctcatcc ctgacggaaa tggcgatacg gctggcgccg
     3121 atccggcatc tggccgttgc gcgggaggtt tgcatcgcgt gagcaagggg tggctttccc
     3181 gtctgggggt acggagccgg ctggcgcgtc tggccggatc ggccgtgctt gctgccgggc
     3241 tgctgggccc atcgggcacg cagacggcga tcgccaacgg cgacacccgc tcgctcaccc
     3301 tctatcattc gcatacccag gaaaacatca ccgtcacttt ccgccgcgac ggctcctacg
     3361 atcggtcggc gctggaaaag ctgaactggt tcctgcgcga ctggcgcaac gacgatgcaa
     3421 cccagatgga cccgcgcctg ttcgatgtcg tctgggccac ctggcgtgaa gtcggatcgg
     3481 gcgccgcgat ccgcattgtt tcggcttatc ggtcgcccgg aaccaacgcc atgctccgcc
     3541 gccgatcctc cggtgtggcc aagaacagcc agcacatcca gggcaaggcc atggacctgc
     3601 acatgcccga tgtgtcgatg gccaaggtgc gcgagatcgg catgcgtctg cagcgtggcg
     3661 gcgtcggcta ttaccccacg gcgggcacgc ccttcgtgca tctcgatgtc ggatcggtgc
     3721 gatcctggcc gcgtatgccg cgcaatcagc ttgaacggct gtttcccgat ggcaagaccg
     3781 tgcatattcc cgccgacggc actccgctgg cgggctacga ggcggcgctg gcggaaatcc
     3841 agacacgcgg cggctcggcg ctgtcctatg ccgagatcac cagcccgcgc accgcaggat
     3901 cggcctctgc cgcctccacc gcc
//
LOCUS       DAOMXG010000201         3892 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_12716_length_3892_cov_8.505603, whole genome shotgun sequence.
ACCESSION   DAOMXG010000201 DAOMXG010000000
VERSION     DAOMXG010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12716_length_3892_cov_8.505603"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            353..1570
                     /locus_tag="PLQ11_11235"
     CDS             353..1570
                     /locus_tag="PLQ11_11235"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019904630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HRK25515.1"
                     /translation="MQEFHRVRRLPPYVFEQVNKIKAQARANGADIIDLGMGNPDMPA
                     PKHVIEKMIETVGKPRTDRYSASKGIPGLRRAQANYYARRFGVKLNPDTQVIATLGSK
                     EGFANMAQAITGPGDVVLVPNPSYPIHAFGFLMAGGVIRSVPSAPDEEYFRALERAVI
                     HSIPKPIAIVTCFPSNPTAEVCDLDFYKELVSFARKHELIVLSDIAYAEVYFDDNNPP
                     PSILQVPGAFDVAVEFSSMSKTFSMAGWRMGFCVGNEKLCAALGRVKSYLDYGAYTPI
                     QVAATAALNGPEDCIREMRDTYRKRRDAMVESFRNAGWDIPAPSASMFAWVPIPDKFK
                     AMGSLEFSKLLVEKAEVAVAPGIGFGEHGDDFVRLALVENEHRIRQAARNIGKFFKTA
                     DATLHNVVTLKTA"
     gene            complement(1647..1943)
                     /locus_tag="PLQ11_11240"
     CDS             complement(1647..1943)
                     /locus_tag="PLQ11_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRK25516.1"
                     /translation="MLERAYWVYMLSSKPNGTLYIGVTNDLGRRVYEHKTKAVRGFTA
                     DYDVSMLVWYESHADINEAIAREKMLKKWRRGWKIRLILEMNPEWDDLYQTLNH"
     gene            complement(2077..2202)
                     /locus_tag="PLQ11_11245"
     CDS             complement(2077..2202)
                     /locus_tag="PLQ11_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25517.1"
                     /translation="MKTVEVKAGAVISGVIKAEVGERGISNKGVKGCETCIIEMD"
     gene            2952..>3892
                     /locus_tag="PLQ11_11250"
     CDS             2952..>3892
                     /locus_tag="PLQ11_11250"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010659878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HRK25518.1"
                     /translation="MAEALRVGIAGLGTVGVGALRILTTRANELALAAGRPITVTAVS
                     SRDRSRDRGIDLKSYRWFDNPVDLARADDIDVFVETIGGAEGQAHEAVKAALNAKKPV
                     VTANKALLARHGADLARLAEKNGVVIAFEPAAAGGIPVIKALKESLVGNTITRVSGIM
                     NGTCNYILSRMEAEGLTFAECLKDAQRLGYAEADPTFDVEGFDTAHKLALLTALAFGT
                     EVDAEAVYVEGISGVTPLDLKMADELGFRIKLLGVAERTAQGIEQRVHPTMVPKSSQL
                     AQVMGVLNAVSVLADPVGELTFVGPGAGAGATGSAVVG"
BASE COUNT          710 a         1253 c         1194 g          735 t
ORIGIN      
        1 caccttgggg ccggtatagg ccgccgccgc cacctccgcc ggcctatacc ggccccaagg
       61 tggatgttcc ggccgccgcg caggctgacc cgtcgcctga acagccaaag gcgaaatcca
      121 ccaaaaagaa accaaacaac aagaaagctc cggcaagatc agcctcgccg ggcacctgac
      181 agccagcccc ggcgatccgg ccgcctcttc gcgatcctga aagttctggc gcgggaaggg
      241 gacttcgcat atggagcgga gcacggatcg aacgcgcgtc gaggagtttg atcccgtgtc
      301 agagttgtat cgtcccgcct tcggacagcg ccaaaccgag agctttttcg agatgcagga
      361 atttcaccgc gttcgtcgtc tgccgcccta tgttttcgag caggtcaaca agatcaaggc
      421 ccaggcgcgt gccaacgggg ctgacatcat cgacctcggc atgggcaatc cggacatgcc
      481 ggcgcccaag cacgtcatcg agaagatgat cgagaccgtg ggcaagccgc gcaccgatcg
      541 ctattcggcc tccaagggca ttcccggcct gcgccgcgcc caggccaatt actacgcccg
      601 ccgctttggc gtgaagctga acccggacac ccaggtcatc gccacgctcg gctccaagga
      661 gggctttgcc aacatggcgc aggccatcac cggtccgggc gatgtggtgc tggtgccaaa
      721 cccgtcctat ccgatccatg ccttcggctt cctgatggcc ggcggggtga tccgctcggt
      781 gccgtcggcg ccggacgagg aatatttccg cgcgctggag cgggcggtga tccactcgat
      841 ccccaagccg atcgcgatcg tcacctgctt cccgtcgaac ccgacggccg aggtctgtga
      901 tctcgacttc tacaaggagc ttgtctcctt cgccaggaag cacgaattga ttgtcctgtc
      961 cgatatcgcc tatgccgaag tctatttcga cgacaacaac ccgccgccgt cgattcttca
     1021 ggttccgggc gctttcgatg tggcggtcga gttttcctcg atgtccaaga ccttctcgat
     1081 ggccggctgg cggatgggct tctgcgtcgg caacgagaaa ctgtgcgccg cgctcggccg
     1141 ggtgaaatcc tatctggatt atggcgccta tacgccgatc caggtggcgg cgaccgccgc
     1201 gctcaacgga cccgaggatt gcatccgcga gatgcgtgat acctatcgca agcgccgcga
     1261 tgcgatggtc gagagctttc gcaatgccgg ctgggacatt cccgcgccct ccgcctcgat
     1321 gttcgcctgg gtgcctatcc cggacaagtt caaggcgatg ggcagcctcg aattctccaa
     1381 gctgctggtc gagaaggcgg aagtcgccgt cgccccgggc attggctttg gcgagcatgg
     1441 cgatgatttc gtccgtctcg ctctggtcga gaacgagcac cgcatccgcc aggcggcgcg
     1501 caacatcggc aagttcttca agaccgccga cgcaacgctg cacaacgtgg tgacgctcaa
     1561 gacggcgtga ggggaaccgg cgtcattccg ggctccgctt cgcggccccg gaatgacacc
     1621 gcagtcgtgc tggatgacgc cggcgctcaa tggttcagtg tctggtagag gtcgtcccac
     1681 tccgggttca tctccaggat caggcggatt ttccatccgc gccgccattt cttcaacatc
     1741 ttttcgcggg cgatggcttc gttgatgtcg gcatgcgact cataccagac cagcatcgag
     1801 acatcataat cggcggtgaa acccctcacg gccttggtct tgtgttcata cacgcggcgg
     1861 cccagatcgt tggtgacgcc gatatagagc gttccgttgg gtttgctgga gagcatatag
     1921 acccagtagg cgcgctccaa catttgcctc ccctgtcatt ccggggcggc gcagccgagc
     1981 ccggaatcca tcttccgcag cctattggcc tgcaccacac ttgcaagatg gattccggac
     2041 ttggccaagg ccaatccgga atgacagcgg cagcgttcaa tccatctcga tgatgcaggt
     2101 ttcgcagccc ttgacgccct tgttggatat acccctctcg ccgacctcgg ccttgatgac
     2161 gccgctgatc accgcgccgg ccttgacctc gacggttttc aacaagcgct cgccggcaaa
     2221 tatcttgtaa gcgccggcct ccttcgcgac gacgcggaac cggccatcgg cgttggcggc
     2281 ggtgtgtttg gcagtcgctc cggggtcttt cttgacaatg acgtcgacgc ccttaactgg
     2341 ctttttctgc gcaaacgcag gcccgacagc gaacaaaagg gcgaggcgac ggcgacaaat
     2401 ttctacacga tatcctcctg cttgcaatgt tcgatggcca tatcggcaag ttcaaccatg
     2461 ccccgcgcca gttgaacagg gactgatacc agcggccctg acaattgacc gtcaggggca
     2521 attgtcaggc ggctggtcag gagcgaatgc gacgccgagc agggccgaag gcccggttgc
     2581 gagggatggc ccgggaaatg accgacaggt catttgtgag ggccactggt atgaaataga
     2641 atttgtctgc cgttcatgtg gatttctcga ccgcgcacat tctgctgtca ttccggggcc
     2701 gcgaagcgga gcccggaatc catctccgag gtttgcttcg caaaccagcc acgccttctt
     2761 ttgcacatcc cgtgcgcgac agctccttcg ccgggcgaag gagcgaaagc ctgttctatt
     2821 tcaccgctgc tgcaaagatg gattccggac aagcctgacg gctttccgga atgacactcc
     2881 tctcgccatt gccccgccgc gccaactccg ctaatcgatc ctcttgctgc atcgcacata
     2941 agagacaggt catggctgag gcgcttcggg taggcatcgc tgggttgggc acggtcggcg
     3001 tcggggcgct gcgcattctg accacccgcg ccaatgagct ggcgctggcc gccggccggc
     3061 cgatcacggt caccgccgtg tcctcgcgcg accggagccg cgatcgcggc atcgacctca
     3121 aatcctatcg ctggttcgac aacccggtcg atctcgcccg cgcggacgac atcgacgtgt
     3181 tcgtcgagac catcggcggc gccgaggggc aggcgcacga ggcggtcaag gccgcgctga
     3241 atgcgaagaa gccggtggtc accgccaaca aggcgttgct cgccaggcac ggcgctgacc
     3301 tcgcccgcct tgccgagaag aatggcgtgg tgatcgcctt cgagccggcg gcggcgggcg
     3361 gcattccggt tatcaaggcg ctgaaggaga gcctggtcgg caacacgatc acccgcgtct
     3421 ccggcatcat gaacggcacc tgcaactaca tcctcagccg catggaagcg gaggggctga
     3481 cgtttgccga gtgcctgaag gatgcccaac ggcttggcta tgccgaggcc gaccccacct
     3541 tcgatgtcga ggggttcgat acggcgcaca agctggcgct gctgacggcg ctcgccttcg
     3601 gaaccgaggt cgatgccgag gcggtctatg tggaaggcat ttctggtgtc acgccgctcg
     3661 atctgaagat ggccgatgag ctcggcttcc gcatcaagct gctcggcgtc gccgaacgca
     3721 ccgcgcaggg catcgagcag cgcgtgcatc cgacgatggt gccgaaatcc tcgcagcttg
     3781 cgcaggtgat gggcgtgctc aacgccgtct ccgttctggc cgatccggtg ggcgagctca
     3841 ccttcgtcgg ccccggcgcc ggggcagggg ccaccggcag cgccgtggtg gg
//
LOCUS       DAOMXG010000202         3883 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_12757_length_3883_cov_9.816353, whole genome shotgun sequence.
ACCESSION   DAOMXG010000202 DAOMXG010000000
VERSION     DAOMXG010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12757_length_3883_cov_9.816353"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="PLQ11_11255"
     CDS             <1..261
                     /locus_tag="PLQ11_11255"
                     /inference="COORDINATES: protein motif:HMM:NF014968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AprI/Inh family metalloprotease inhibitor"
                     /protein_id="HRK25519.1"
                     /translation="LRKGERDSGCRLTLAIGATPEGSATLATGCEDRGVLSFAPARWH
                     MAGGTLWLTGGRGRLSFERNRKGGWDKAPGQGEALLLAPEAP"
     gene            complement(280..1416)
                     /locus_tag="PLQ11_11260"
     CDS             complement(280..1416)
                     /locus_tag="PLQ11_11260"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRK25520.1"
                     /translation="MPADLSRWTASLRPDGRVLEGTHCRLEKLDAARHGADLWAAIEN
                     HDSLWDYLSYGPWPDRAAFLDWLGQRQTLVDPLYYAVVDRHSERALGVITLMEIRPES
                     GVIEVGHIFYSPLLQRTPAATEAIHLAGRHVFRDLGYRRFEWKCNNANEASRRAALRF
                     GFVAEGVFRQHMVVKGRNRDTAWFSIIDRDWALAERAFERWLAPGNFGPRGEQKIALG
                     QINIGDIDVGSLKLARVGVVDRDEIEAFQREAYARNREILGAEPLPLQWDYGRIFHET
                     EVWGVRDKGKLVGVLILRPRADDLYLESVATLPQVQGGGYGNAMLTATEFRARDWGYR
                     TIRLVTGEALTRNVDWYLRKGFHVEEIERQPDRNLVHMIKHLAD"
     gene            complement(1683..3491)
                     /locus_tag="PLQ11_11265"
     CDS             complement(1683..3491)
                     /locus_tag="PLQ11_11265"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004438887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HRK25521.1"
                     /translation="MSSSKPTPAKIRKLRSRAWFNNPDNIDMTALYVERMLNFGMTIE
                     ELQSGKPIIGIAQTGSDITPCNRHHIELAKRTREGIREAGGICCEFPIHPVQETGKRP
                     TASLDRNLQYLSLVEVLYGYPIDGIVLTTGCDKTTPAQIMAAATVDIPAIALPVGPML
                     NGWFKGERTGSGTIVWKAREELAKGAINHQQFIEIVASSTPSVGFCNTMGTASTMNSL
                     AEAMGMTLPGAAAIPAAYRDRMQCAYWTGKRIVEMVWEDLRPSKILTREAFENTIRVN
                     SAIGGSTNAPIHVNAIARHIGVTLTNDDWERLGYDIPLLVNLQPAGEYLGEDYYRAGG
                     VPAVVAELIAKGKISRTPITANGKTLHENCEGRFAENRDVIKTYDKPMKSSAGFLNMK
                     GNLFDSAIMKTSVISEEFRARYLSNNKDPNAFEGKAAVFDGPEDYHKRIDDPALKLDA
                     NTVLIMRGTGPVGYPGAAEVVNMRPPAYLLKRGINALPCIGDGRQSGTSGSPSILNAS
                     PEAATGGGLALVRMGDPIRIDLNTRTVTLLISDAEMAQRRAELKKKRYKFPGHQTPWQ
                     EIQRGMVDELSNGMVLKPAVKYQRIDKTKGIPRDNH"
     gene            3547..>3883
                     /locus_tag="PLQ11_11270"
     CDS             3547..>3883
                     /locus_tag="PLQ11_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015951103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25522.1"
                     /translation="MLLKRTGSIVRQDQDAEADGTLDCFDSELRRRSAANATGNREFV
                     CYQTTKPARSEPAMLDIVPAFGRIGEENAFAVLARATELQRQGKDIINLGIGQPDFAT
                     PPNIVEAAIR"
BASE COUNT          694 a         1258 c         1193 g          738 t
ORIGIN      
        1 ctgcgcaaag gcgagcgcga tagcggttgc cggcttacgc tggcgatcgg tgccacgccc
       61 gaaggcagcg cgacgctggc caccggctgc gaggatcgcg gtgtcctttc cttcgccccc
      121 gcgcgttggc acatggccgg cggcacgctg tggctgacag gcggccgcgg ccgactgagc
      181 ttcgaacgca atcgcaaggg cggctgggac aaggcccccg gccagggcga ggcactgctg
      241 ctcgcccccg aggcgccctg atcgtcccgc gcccgcgcct caatcggcga ggtgcttgat
      301 catgtgcacc aggttgcgat ccggctgccg ttcgatctcc tcgacatgga agcccttgcg
      361 cagataccag tcgacattgc gcgtcagcgc ctccccggtc accagccgaa tggttcggta
      421 gccccagtcg cgggcgcgga attcggtcgc cgtcagcatg gcgttgccat agcccccgcc
      481 ctgcacctgc ggcagggtgg cgacgctttc gagatacagg tcgtcggcgc gcggcctcag
      541 gatcagcaca ccgaccagct tacccttgtc tcgaacgccc cacacttcgg tctcgtgaaa
      601 gatgcgcccg tagtcccatt gcagcggcag cggttcggcg ccgagaatct cgcggttgcg
      661 ggcataggct tcgcgctgga acgcctcgat ctcgtcccgg tcgacgactc ccacccgcgc
      721 cagtttcagc gagccgacgt cgatgtcgcc gatattgatc tggccgagcg cgatcttctg
      781 ctcgccgcgc ggtccgaaat tgcccggcgc cagccagcgc tcgaacgccc gctcggccag
      841 ggcccagtcg cggtcgatga tcgagaacca cgccgtgtcc cggttccgac ccttcaccac
      901 catgtgctgg cggaacaccc cttcggccac gaaaccaaag cgcagggcgg cccgcctgga
      961 cgcctcgttg gcattgttgc acttccattc aaaccgccgg tagccgagat cacggaatac
     1021 atggcgaccg gcgagatgga tcgcctcggt cgccgccggg gtgcgttgca gcagcggcga
     1081 atagaaaatg tggccaacct cgatcacgcc cgattcgggg cggatttcca tcagcgtgat
     1141 gacgccgagc gcccgttccg agtgccggtc gaccaccgca taatagagcg gatcgaccag
     1201 cgtctgacgc tggccaagcc agtcgagaaa cgccgcgcga tccggccacg gtccatagga
     1261 caaatagtcc cacaggctgt catgattctc gatcgccgcc cacaggtcgg cgccatgccg
     1321 ggcggcatcc agcttttcca gccggcaatg cgtgccttcc agcacgcggc catcgggacg
     1381 aagcgaggcg gtccagcggg aaagatcggc aggcattgtc gcaaacacct catgctgtct
     1441 gccgttgcag cgcccccaga ttgaggtccg gcaacaaagc agaacaggag cctggagcat
     1501 gctgcgatta agtggaagcc gcttaatcgc cggagcatgc tctaaactat tgataatgat
     1561 cacgttatct cacgttttga tgattccatc aaaatgcggc gtaatctaga gacaaaatcg
     1621 cggcagagcg agcggcaagg atgccgcacc cttggcgcaa gcgccgtttg acccgcgctg
     1681 gatcaatggt tgtcgcgagg gatgcccttg gtcttgtcaa tcctctggta tttgaccgcc
     1741 ggtttcagca ccatgccatt ggacaattca tcgaccatgc cgcgctggat ttcctgccac
     1801 ggcgtctggt gaccggggaa cttgtagcgc ttcttcttca gctccgcccg ccgctgcgcc
     1861 atttccgcat cggaaatcag cagggtgacc gtgcgcgtgt tgaggtcgat gcggatcgga
     1921 tcacccatcc tgaccagcgc cagcccgccg ccggtcgccg cttccggcga ggcattgagg
     1981 atcgatggcg accctgacgt acccgactgc cggccatcgc cgatacaggg caacgcattg
     2041 atccctcgct tgagcagata ggccggcggg cgcatgttca caacttccgc cgcgccaggg
     2101 tagccgaccg gcccggtgcc gcgcatgatc agcacggtgt ttgcatcgag cttgagcgcc
     2161 ggatcgtcga tccgcttgtg gtaatcctcc ggcccatcga acaccgccgc cttgccctcg
     2221 aaggcattgg ggtccttgtt gttggacaga tagcgggcgc ggaattcctc ggatatgacc
     2281 gaagtcttca tgatcgccga atcgaacagg ttgcccttca tgttgaggaa cccggccgag
     2341 gatttcatcg gcttgtcgta agtcttgatg acgtcgcggt tttccgcaaa ccggccttcg
     2401 caattttcat gcagcgtctt gccgttggcg gtgatcggtg tcctgctgat cttgcccttg
     2461 gcgatcagtt ccgccaccac cgccggcacg ccgccggcgc ggtaataatc ctcgccgaga
     2521 tactcgcccg ccggctgcag attgaccagg agcgggatat cgtagccgag ccgctcccag
     2581 tcgtcgttgg tgagcgtcac cccgatatgg cgggcgatgg cgttgacatg aatcggcgca
     2641 ttggttgagc cgccgatggc cgaattgacg cggatcgtgt tctcgaacgc ctcgcgggtg
     2701 aggattttcg aagggcgcag atcctcccac accatctcga cgatgcgctt gccggtccag
     2761 taggcgcatt gcatccggtc gcggtaggcg gcgggaatgg cggccgcccc cggcagggtc
     2821 atgcccatcg cctcggccag cgaattcatc gtcgaggccg tgcccatcgt attgcagaac
     2881 ccgactgatg gcgtcgacga ggcaacgatc tcgatgaact gctgatggtt gatcgcgccc
     2941 ttggcgagtt cctcgcgcgc cttccagacg atcgtgcccg aacccgtgcg ctcgcccttg
     3001 aaccaaccgt tgagcatcgg tcccaccggc agcgcgatcg ccggaatatc caccgtcgcc
     3061 gccgccatga tctgcgccgg cgtggtcttg tcgcagccgg tggtcagcac gattccgtcg
     3121 atcggatagc cgtaaagcac ctcgaccagc gacagatatt gcagattgcg atcaagactg
     3181 gccgtcggcc gcttgccggt ctcctgcacc ggatgaatcg gaaattcgca gcagattccg
     3241 cccgcttccc ggataccttc gcgggtgcgt ttggcaagct cgatatggtg ccggttgcac
     3301 ggcgtgatgt ccgatccggt ctgggcgatg ccgatgatcg gcttgcccga ctgcaactcc
     3361 tcaatcgtca tgccgaaatt gagcatgcgt tcgacataga gcgcggtcat gtcgatattg
     3421 tcgggattgt tgaaccaggc gcgggaccgg agtttgcgga tcttggcggg cgtgggcttg
     3481 gatgacgaca tggtgctggg acctgcttgt gttctggctg cggcgtcagg gcgaacccgc
     3541 cgggccatgc tgctgaaacg gacgggcagc attgtccgac aggaccagga tgccgaagca
     3601 gacggaacgc tcgattgctt cgatagcgaa cttcgcagaa ggtccgctgc gaatgcaact
     3661 ggcaatcgcg aatttgtctg ttatcagaca acaaaacctg cccgttcgga gcctgccatg
     3721 ctcgacatcg tccctgcttt cggccgcatc ggtgaagaaa acgccttcgc cgtgcttgcc
     3781 cgcgccaccg aactgcaacg tcagggcaag gacatcatca acctcggcat cggccagccg
     3841 gatttcgcca cgccccccaa catcgttgag gccgccatca ggg
//
LOCUS       DAOMXG010000203         3872 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_12823_length_3872_cov_10.646319, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000203 DAOMXG010000000
VERSION     DAOMXG010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12823_length_3872_cov_10.646319"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..758)
                     /locus_tag="PLQ11_11275"
     CDS             complement(54..758)
                     /locus_tag="PLQ11_11275"
                     /inference="COORDINATES: protein motif:HMM:NF013315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRK25523.1"
                     /translation="MSAGSAAAWSSVAPDPAGMPDAAAPDAALAQFIMAMRARGVRDK
                     NLLVAFEKTLRGAFLPRTNPRLVYAPYALPLDCGAEATPPHLIAEVLGTLELQPGMRV
                     LDIGTGSGYQAGVLAQFGCQVVTLERYHTLHLNAARAMRRLGIVNVRLEHCDGREGFA
                     EAGPYDRILVNAAVRDVPDALIEQLNPGGTIVIPVQHGREQRLYAFEDVGDTFRQRDF
                     GRSTFQMMQDGRALAL"
     gene            complement(755..1519)
                     /gene="surE"
                     /locus_tag="PLQ11_11280"
     CDS             complement(755..1519)
                     /gene="surE"
                     /locus_tag="PLQ11_11280"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HRK25524.1"
                     /translation="MRILITNDDGIHAEGLDSLERIARALTDDVWVIAPESDQSGVAH
                     SLSLNDPLRLRQISEKRFAVKGTPTDCVIMGIRHVMREARPDLVLSGVNKGQNVAEDV
                     TYSGTIAGAMEGTLLGIASIAVSQAYPFKNRAGIRYEVAEKFGPDLIRRILALGIPEG
                     VLINLNFPSVPDEEVRGVRVTVQGRRDAEIMKIDARHDGRGHPYYWIGFNPARQNPAE
                     GTDLAAMQDGYISITPLRLDLTDMPTVTRLAEAFDG"
     gene            complement(1535..2812)
                     /gene="serS"
                     /locus_tag="PLQ11_11285"
     CDS             complement(1535..2812)
                     /gene="serS"
                     /locus_tag="PLQ11_11285"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRK25525.1"
                     /translation="MHDIRFIRDHAAAFDEALNRRGLAPQSRALLDLDDARRKAITGA
                     ETALARRNAASKEIGQAMAQKDAARAEALKAEVATLKDRIAALELEQQTAQKALDEAL
                     AVIPNMPLADVPVGRDETGNVEVRKWGTPPAMDFAPKQHFELGEALGMMDFEAASRMS
                     GARFVVLKGPLARLERALGQFMLDLHTGEHGYREIQAPLLVKDAALYGTAQLPKFRED
                     LFGVQHDFSLIPTSEVSMTNLVMGQIVDEAELPYRFTGLTPCFRSEAGSAGRDTRGML
                     RQHQFNKVELVSITAPEQSLDEHARMTRCAETVLEKLGIAYRTMVLCTGDMGFGSQKT
                     HDIEAWLPGQGTYREISSCSVCGDFQARRMNARCKPKDGKGTRFVHTLNGSGVAVGRA
                     LIAVMENYQTADGAIVVPEMLRPYMGGLDRITK"
     gene            complement(2908..3705)
                     /gene="tatC"
                     /locus_tag="PLQ11_11290"
     CDS             complement(2908..3705)
                     /gene="tatC"
                     /locus_tag="PLQ11_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HRK25526.1"
                     /translation="MTELPRPGQEDIDASKAPLIEHLIELRTRLITALMAFLAMTIFC
                     FAIAKYIYGVLVWPYIWAAGGPEKAPLIYTHPLEYFFVQLKLAVFGGAFLAFPVIATQ
                     IYKFVAPGLYKDERDAFRPFLIATPILFFLGALVVMTVAMPLLMKFSLAMQMTVADGG
                     SNISLLPKVDDYLSLIMTLIFAFGITFQLPVVLTLLGRVGLIDSEFLREKRRYAIVIV
                     FVIAAVLTPPDIISQFSLAIPGMLLYELSIYSVKWAEKKHANGSDQS"
BASE COUNT          722 a         1260 c         1232 g          658 t
ORIGIN      
        1 aggttttcgc attttcttga cgcgaaccgg aatccacttc gttcgaaaat gctctatagc
       61 gccagcgccc gcccgtcctg catcatctgg aaggtggagc ggccgaaatc gcgctggcgg
      121 aaggtgtcgc cgacatcctc gaaggcatag agccgctgct cgcgcccatg ctgcaccgga
      181 atgacgatgg tgccgcccgg attcaattgc tcgatcagcg cgtcaggcac atcgcgcacg
      241 gcggcattga ccagaatgcg gtcgtagggc cccgcctcgg cgaagccctc gcgcccgtcg
      301 caatgctcca ggcggacatt gacgatgccg aggcgccgca tggcgcgcgc cgcattgaga
      361 tgcagcgtgt gatagcgctc cagcgtcacc acctggcagc cgaactgggc cagaacgccg
      421 gcctgataac ccgatccggt gcctatgtcg agcacacgca tgcccggctg aagctccagc
      481 gtgcccagaa cctcggcgat cagatgcggc ggtgtcgcct cggccccgca atcgagcggc
      541 agggcatagg gcgcatagac aaggcgcgga ttggtgcgcg gcaggaaggc gccgcgcaag
      601 gtcttttcaa acgccaccag caggttcttg tcgcgcacac cgcgcgcgcg catcgccatg
      661 atgaactgcg cgagcgcggc atccggtgcc gcagcgtcag gcatgccggc cggatcgggg
      721 gcgacagacg accaggccgc agcgctcccc gcactcatcc gtcgaaagcc tccgcaagcc
      781 gggtgacggt gggcatgtcg gtcaggtcga gcctgagcgg cgtgatggaa atgtagccgt
      841 cctgcatcgc cgccagatcg gtgccttccg ccgggttctg ccgcgccggg ttgaagccga
      901 tccagtaata gggatggccg cgcccgtcgt ggcgcgcgtc gatcttcatg atctcggcat
      961 cgcgccggcc ctgcacggtg accctgacgc ccctgacctc ctcatccggc acggagggga
     1021 agttgaggtt gatcagcacg ccctccggga tgcccagcgc cagaatgcgg cggatgagat
     1081 cgggaccgaa cttctccgcc acctcgtagc ggatgccggc gcggttcttg aacggatagg
     1141 cctgcgacac ggcgatcgag gcaatgccga gcaaggtgcc ttccatcgcc ccggcgatgg
     1201 tgccggaata ggtgacatcc tcggcgacgt tctgcccctt gttgacgccg gacaatacca
     1261 gatcgggccg cgcctcccgc atcacgtggc ggatgcccat gatgacgcaa tcggtcggcg
     1321 tgcctttgac ggcaaagcgc ttctccgaga tctgtcgcag ccgcaaagga tcgttgagcg
     1381 acagcgaatg ggcaacgccg ctctggtcgc tctcgggcgc gatgacccag acgtcatcag
     1441 tcagcgcgcg ggcgatacgc tcgaggctgt ccagcccctc ggcatgaatg ccgtcgtcat
     1501 tggtgatcag gattcgcatc ccgtcaccct tgcctcactt tgtgattctg tcgagaccac
     1561 ccatgtaggg ccgcagcatt tccggcacga caatcgcgcc atcggcggtc tggtagttct
     1621 ccatcaccgc gatcagcgca cggccgaccg ccacacccga gccgttcagc gtgtggacaa
     1681 agcgcgtgcc cttgccatcc ttcggcttgc agcgggcatt catgcgccgg gcctggaaat
     1741 cgccgcagac cgagcaggag gaaatctcgc gataggtgcc ctgccccggc agccaggcct
     1801 cgatatcgtg ggtcttctgg ctgccaaatc ccatgtcgcc ggtgcacagc accatggtcc
     1861 gatacgcgat gccgagtttt tccagaaccg tctcggcgca gcgcgtcatg cgcgcgtgct
     1921 cgtcgagcga ttgttcgggc gcggtgatcg acaccagttc caccttgttg aactgatgct
     1981 ggcgcaacat gccgcgcgta tcgcgcccgg ccgatcccgc ttccgaccga aagcagggcg
     2041 tcagcccggt gaagcgatag ggcagttccg cctcatcgac gatctggccc atgaccagat
     2101 tggtcatcga cacttcggag gtggggatga gcgaaaaatc atgctgcaca ccgaacagat
     2161 cctcgcggaa cttcggcaat tgagccgtgc cgtaaagcgc ggcgtccttg accagcagcg
     2221 gcgcctgaat ctcgcgatag ccatgctcgc cggtatgcag atcgagcatg aactggccga
     2281 gcgcgcgttc gagccgcgcc agcgggcctt tcagcacgac aaagcgcgcg ccggacatgc
     2341 ggctggccgc ctcgaaatcc atcatgccaa gggcttcgcc gagttcgaaa tgctgcttgg
     2401 gcgcaaaatc catcgccggc ggcgtgcccc acttgcgcac ctcgacattg ccggtctcgt
     2461 cgcgaccgac cggcacatcg gccaacggca tgttggggat gacggcaagc gcctcgtcga
     2521 gcgccttctg cgcggtctgc tgttcaagct cgagcgcggc gatccggtcc ttcagcgtgg
     2581 cgacctccgc cttcagcgcc tcggcgcggg cggcatcctt ctgcgccatc gcctggccga
     2641 tctccttgga ggcggcattg cgacgggcaa gcgccgtctc ggccccggtg atcgccttgc
     2701 ggcgagcatc atcaagatca agcagcgccc gcgattgcgg cgccaacccg cgccggttga
     2761 gggcctcgtc gaaggcagcg gcatggtcgc ggatgaaacg gatgtcgtgc atggcgcaaa
     2821 aaggatcgca aacagggtcg gaaacggtgg cgctgttaga ccgctgttgc ccgccaagag
     2881 caagggcgaa attgtcgccg cagtgtgtca gctctggtcg ctgccgttcg catgcttctt
     2941 ctcggcccat ttgaccgagt agatcgacag ttcgtagagc agcatcccgg ggatcgccag
     3001 cgagaactgg ctgatgatgt ccggcggtgt cagcacggcg gcgatgacaa acacgatgac
     3061 gatggcataa cgccgctttt ccctgaggaa ttcgctgtcg atcagcccga cgcgccccag
     3121 cagggtcagc accaccggca gctggaaggt gatgccgaag gcgaagatca gcgtcatgat
     3181 cagcgacagg taatcatcga ccttgggcag cagcgagata ttcgagccgc catcggccac
     3241 cgtcatctgc atcgccagcg agaatttcat cagcagcggc atcgccaccg tcatcaccac
     3301 cagcgccccc agaaagaaca ggatcggcgt ggcgatgagg aacgggcgga aggcatcgcg
     3361 ctcgtccttg tagaggccgg gagccacgaa tttgtagatc tgcgtcgcga tcaccggaaa
     3421 cgccagaaac gccccgccaa acaccgcaag cttgagctgc acgaagaaat attcgagcgg
     3481 atgggtatag atcagcggcg ccttctccgg cccgcccgcc gcccagatat agggccagac
     3541 cagcacgccg tagatatatt tggcgatggc aaaacagaaa atggtcatgg ccagaaaggc
     3601 catcagcgcc gtgatcagcc gtgtgcgcaa ttcgatgaga tgctcgatca acggagcttt
     3661 ggaggcgtca atatcctcct gcccgggccg tggcagttcg gtcatgcggt gtctccgcct
     3721 gccttcgagc ccgccgcctt ggtccgcttc tttgccgggg cggcctcaac ggcaggagcg
     3781 gcagcagccg cagcaggggc aaccggcgat ggcgctggct cgatcgccgg cgggccgacc
     3841 tcgggcacgg gatcgagcgc tgtcaccggc gg
//
LOCUS       DAOMXG010000204         3854 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_12927_length_3854_cov_10.747565, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000204 DAOMXG010000000
VERSION     DAOMXG010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3854
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12927_length_3854_cov_10.747565"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(411..1808)
                     /locus_tag="PLQ11_11295"
     CDS             complement(411..1808)
                     /locus_tag="PLQ11_11295"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HRK25527.1"
                     /translation="MPVKSWSPDSWRHLSIQQVPDYPDAEALKATEAQLASFPPLVFA
                     GEARKLKKLLAKVAAGEAFLLQGGDCAEAFAEHSADNIRDFFRVFLQMAVVTTFASTM
                     PVVKVGRIAGQFAKPRSSPTETDKATGLTLPSYRGDIINGMDFTPEARIPDPRRQIEA
                     YRQSAATLNLIRAFATGGYANLDNAKRWMLGFVDGTPQSGRYRQLVDKITHALDFMRA
                     IGIDPETHPEMRATDFYTSHEALLLGYEQALTRIDSTSGDWYATSGHMLWIGDRTRQP
                     DHAHLEFCRGVKNPIGIKCGPSLTPDGLMQLIDILNPEDEAGRLTLICRFGADKVMDH
                     LPALARRVKAEGRTVVWSCDPMHGNTITAAGGYKTRPFDRILQEVKNFFAIHEAEGTY
                     AGGVHLEMTGKNVTECTGGARALSEAALGDRYDTQCDPRLNAEQSIEMAFLIADLLNH
                     ERQVRQPSRPAAAAE"
     gene            complement(1919..3298)
                     /gene="gor"
                     /locus_tag="PLQ11_11300"
     CDS             complement(1919..3298)
                     /gene="gor"
                     /locus_tag="PLQ11_11300"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HRK25528.1"
                     /translation="MSGFDVDFCVIGGGSGGVRSARIAAGYGAKVLLAEEFRMGGTCV
                     IRGCVPKKLYVYASRFSHEFEEAAGFGWSVPNASFNWPTLVKAKEAEITRLEGLYARG
                     QASAGVEVVKSRAVFEDAHTVRLVADSRLIRARHILIATGGRPFLPAIPGVEHAITSS
                     EAFDLAEQPRRIVIAGGGYIAVEFAGIFAGLGSEVTLVHRGDNILRGFDDDLRNAVKT
                     AYEKRGIRLLLGDTIARIDKGDGLAVTTAKGETIAADQVMLALGRVPNVEGLGLDKAG
                     VALRHNHHAIAVNAASQTSVPHIYAVGDVTDRVNLTPVAIREGHAVADTLFGNKPWTV
                     DHANIATAVFSQPELGTVGLTEAQARDKHRIVDIYRTEFRPMKATLSGSADRVLMKII
                     VDGETDRVLGVHIAGEAAGEMAQLLGIAVRMGATKADFDATMAVHPTAAEELVTLRTR
                     TSRWERDAQ"
     gene            complement(3401..>3854)
                     /locus_tag="PLQ11_11305"
     CDS             complement(3401..>3854)
                     /locus_tag="PLQ11_11305"
                     /inference="COORDINATES: protein motif:HMM:NF021361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HRK25529.1"
                     /translation="PIAAPAPAAPALSASHLASALEVAKLSGMTRSTDTLVPQTIDKA
                     RQLFTQMRPELAAEIDKSIKALQPEFDTLVTESQTIVAQAFGARLSESDLKDVAAFFK
                     SAAGQKFVGAQPGILDDMFRDMEPYTQRLSQTIVDKLREDMKKRGAPL"
BASE COUNT          693 a         1248 c         1227 g          686 t
ORIGIN      
        1 ttagagcatg tcttctgcga ttaaccggta tccacttaat cgcgacatgc tctaacaaaa
       61 ccgtttggtc aaattagcgc gcggatttta agcaatcggc aagaaagcgg gttaatgaag
      121 agtaaaacca gtggccctca caaatgacct gccggtcatt tcccgggccg tccctcgcaa
      181 ccgggccttc ggccctgctc ggcgtcgcat tcgctcctga ccagccgcct gccaattgcc
      241 cctaacggtc aattgtcagg gccgctggta taatacctgc ggtcctcagg actgaccgtt
      301 ccgccttcgg cagatcgggt ccgtttcgga ccgaaatccc tcgcataagc tcggacaccc
      361 gttcgggctt gcagtggtca tcggtcaaac ccggtgacca ctggtataac ctactccgcc
      421 gccgccgccg gacggctggg ctggcgcacc tggcgctcgt gattgagcag gtcggcgatg
      481 aggaaagcca tctcgatcga ctgttcggca ttgaggcgcg gatcgcactg ggtgtcgtaa
      541 cgatccccca gcgccgcctc ggacagcgcg cgggcgccgc cggtgcattc ggtgacgttc
      601 tttccggtca tttcgagatg cacgccgcca gcataggtgc cttcggcctc gtggatggcg
      661 aagaaattct tcacctcctg caggatgcgg tcgaacggcc gggtcttgta gccgccggct
      721 gcggtgatgg tgttgccatg catcggatcg caggaccaga ccaccgtgcg gccttcggcc
      781 ttcacccggc gtgccagcgc cggcaggtga tccatgacct tgtcggcgcc gaagcggcag
      841 atcagcgtca gccggccggc ttcatcttcc gggttgagga tgtcgatcag ctgcatcagc
      901 ccgtccggcg tcagcgacgg gccgcatttg atgccgatcg ggttctttac gccgcggcag
      961 aattccagat gcgcatgatc gggctggcgg gtgcggtcgc cgatccacag catgtggccg
     1021 gaggtggcat accagtcgcc ggaggtggaa tcgatgcggg tcagcgcctg ctcgtagccg
     1081 agcagcagcg cctcatggct ggtgtagaaa tcggtggcgc gcatttccgg atgcgtttcc
     1141 ggatcgatgc cgatggcgcg catgaaatcg agcgcatggg tgatcttgtc caccagctgc
     1201 cggtagcggc ccgactgcgg cgtgccatcg acgaagccga gcatccagcg cttggcattg
     1261 tcgagattgg cataaccgcc ggtggcaaag gcgcggatga ggttcagcgt cgccgccgac
     1321 tggcgatagg cctcgatctg ccggcgcgga tcggggatgc gcgcttccgg ggtgaagtcc
     1381 atcccgttga tgatatcgcc acggtaggaa ggcagcgtga gcccggtcgc cttgtcggtt
     1441 tcggtgggcg atgagcgcgg cttggcgaac tggccggcaa tgcggccaac cttgaccacc
     1501 ggcatggtcg aggcaaaggt ggtgaccacc gccatctgca ggaacacgcg gaagaaatcg
     1561 cggatattgt cggccgaatg ctcggcaaac gcctcggcgc aatcgccgcc ctgcaacagg
     1621 aacgcctcgc ccgccgcgac cttggccagc agcttcttca gcttgcgcgc ctctccggca
     1681 aacaccagcg gcggaaacga tgccagctgc gcctcggtcg ccttcagcgc ctctgcgtcc
     1741 ggatagtccg gcacctgctg gatcgacaga tggcgccagc tatcagggga ccaggacttc
     1801 acaggcatga cgttactcca ccacgcggaa aacaaaaaac ttgccgaaaa gcgccgataa
     1861 cgacgtcgga cggccgagct atagcccaag cactccggct tggcgagggg caaggcgcct
     1921 actgcgcatc gcgctcccag cggctcgtgc gcgtccgcag ggtcaccagt tcttccgccg
     1981 ctgtcgggtg cacggccatg gtggcgtcga agtcggcctt ggtggcgccc atgcggaccg
     2041 cgatgccgag cagctgggcc atttcgccgg cagcctcgcc ggcgatgtgg acgccgagca
     2101 cccggtcggt ctcgccatcg acgatgatct tcatcagcac acggtcggcg ctgcccgaca
     2161 gcgtcgcctt catcgggcgg aactcggtcc ggtagatgtc gacgattcga tgcttgtcgc
     2221 gcgcctgcgc ttcggtgagg ccgacggtgc cgagttcagg ctgcgagaaa accgctgtcg
     2281 caatgttggc gtgatccacc gtccagggct tgttgccgaa cagcgtatcg gccaccgcat
     2341 ggccctcgcg gatggcgacg ggggtcaggt tgacccggtc ggtgacatcg cccaccgcgt
     2401 agatatgcgg caccgaggtc tgcgaggcgg cattgacggc aatcgcatga tggttgtggc
     2461 gcagggcgac gccggccttg tccagcccga gcccctcgac attcggcacg cggccgagcg
     2521 ccagcatcac ctgatcggcg gcgatggtct cacctttcgc tgtggtgacg gccagaccgt
     2581 cgcccttgtc gatgcgggcg atggtatcac ccagcaacag ccggatgccg cgcttctcgt
     2641 aggccgtctt caccgcgttg cgcagatcgt catcgaagcc gcgcaaaatg ttgtcgccgc
     2701 gatgaacaag cgtcacctca ctgccgagcc cggcgaaaat gccagcaaat tccaccgcga
     2761 tatagccgcc gccggcaatg acaatacggc gcggctgttc ggcgaggtcg aaggcttcgc
     2821 tcgaggtgat cgcatgttcg acaccgggga tcgccggcag gaacgggcgg ccgccggtgg
     2881 caatcaggat atgccgggcg cggatgagcc ggctgtcggc aacaaggcgg accgtatgcg
     2941 catcctcgaa cacggcgcgg gatttgacca cctcgacgcc ggcgctggcc tgaccgcgcg
     3001 cataaagccc ttccagccgg gtgatctcgg cttccttggc tttcaccagc gtcggccagt
     3061 tgaagctcgc attcggcacc gaccagccga accccgccgc ctcctcgaat tcatgggaga
     3121 aacgtgaagc atagacgtag agcttcttgg gaacgcagcc gcggatgaca caggttccgc
     3181 ccatgcggaa ctcttccgcc agcagcacct tcgcgccgta tcctgccgca atgcgcgccg
     3241 agcgaacgcc gccggagccg ccaccgatga cacagaaatc aacgtcaaag ccgctcacaa
     3301 tgccccccca acttcctgcc ggcccgatag agtccggcac aggccagaac cggcggcaac
     3361 gaagcggtca cgtcctaccc cgcaagatca gggccccgcc tcacaagggc gcgccgcgct
     3421 ttttcatgtc ctcgcgcagc ttgtcgacga tcgtctggct cagccgctgc gtgtagggtt
     3481 ccatgtcgcg gaacatgtca tcaagaatgc ccggctgggc accgacgaat ttctgccccg
     3541 ccgccgactt gaagaaggcc gcgacgtcct tcaggtcgct ctcgctaagc cgggcgccga
     3601 aggcctgcgc cacaatggtc tgcgattcag tgaccagcgt atcgaattcc ggttgcaggg
     3661 ccttgatcga cttgtcgatc tccgctgcca gttcaggccg catctgggtg aacaactggc
     3721 gggccttgtc aatcgtctgc gggaccagcg tatcggtcga acgggtcatg ccggacaatt
     3781 tggcgacctc aagggccgag gccagatgcg aggctgacaa ggccggagcc gccggggccg
     3841 gcgcagcgat cggc
//
LOCUS       DAOMXG010000205         3818 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_13111_length_3818_cov_9.453362, whole genome shotgun sequence.
ACCESSION   DAOMXG010000205 DAOMXG010000000
VERSION     DAOMXG010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13111_length_3818_cov_9.453362"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..1027
                     /locus_tag="PLQ11_11310"
     CDS             158..1027
                     /locus_tag="PLQ11_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HRK25530.1"
                     /translation="MSTMKGFVLCADDFGLTAGISHGILELLADGRLSATGAMTNRPH
                     WPVFAPALRAFDGRADLGVHLNLTCGAPLTAMADFAPERRLPGLPRILSQAMLRRLPE
                     HEIAHELTAQLDAFELAMGRAPDFIDGHQHVHGLPGIQPIFLSVIQRRYTVGHRPYVR
                     VCGDRVWRILRRGAFAAKALNVRQLTRGLDRRLANLGFATNDGFAGFSDFNPDGDYAR
                     AFASYLAAPGARHLVMCHPGFVDEELPTLDPVLHSRERELEFLASNVFADLCHAAGMA
                     PVRMARAGAALAG"
     gene            1086..1325
                     /locus_tag="PLQ11_11315"
     CDS             1086..1325
                     /locus_tag="PLQ11_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HRK25531.1"
                     /translation="MRIPARHTWLVMPAILALLMTFVVSGISTLRAIGPGPEFLAKWM
                     GSWALSYVVAYPTLLVVMPVVRRILAAIMEPAPKG"
     gene            complement(1338..2609)
                     /locus_tag="PLQ11_11320"
     CDS             complement(1338..2609)
                     /locus_tag="PLQ11_11320"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRK25532.1"
                     /translation="MVRTAFSEQMDRLRTGIFLLAVVLVNFTISRPSPVDIAFALALL
                     MTPLCGQRLTRRGLVFLALILAWWAGLMTSSMPFTYDGEVVYQLTKTSYAMSIGITAC
                     LVNMHWGRQQIERLYKFWIGSACLAATFATIGFVLKIDELIWDGRGKGFFDDPNMYAA
                     FLPPAVLGCIYFILTTKTRRRYIAALALLTIGLMVAFSRVAVAATLLTGLVLTLFLNR
                     KRLAKTIMNLAAVAAVAVIAGAIAVAVIPEFAEKLSDRATLAKDYDVGEMGRLNRYLK
                     AFNLTINDPIGMGVLQFEAIFPEPIHNFWLSSFVNYGWLAGFAWTAVVYFGVILTIRN
                     YRQTGDSLMVLIYLSWLGIFACALLHEAERWRHLWFFTGFLWGVNPHNWRQTVRAPAP
                     ASPPRDIRAAPAMARHLPPHPGLLRQKPSVS"
     gene            complement(2802..3404)
                     /locus_tag="PLQ11_11325"
     CDS             complement(2802..3404)
                     /locus_tag="PLQ11_11325"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HRK25533.1"
                     /translation="MSFTLPDLPYAHDALQPYMSRETLEYHHDKHHLAYVNNGNNLLK
                     GSEWEGKSLEDIVKGSFGKNAGLFNNAGQHYNHLHFWRWMKPNGGGDKLPGRLEKAVI
                     DGFGSVAKMKEDFIQGGITQFGSGWTWLAVKDGKIVVMKSPNGESPLVHGAAPILGCD
                     VWEHSYYIDYRNRRPDYLKAFVDHLVNWDYVDEMFQAAMK"
BASE COUNT          698 a         1159 c         1208 g          753 t
ORIGIN      
        1 gaccgtgagt tccggcgtgc cggaaaaggc gcggggcgaa ggatgggcgt tcaaaggcat
       61 cactttcgat caagacggcg catcgccgct caggtcaagt gtcaaaccgg cctgcggcga
      121 agcagcgccc ttgtgtcacg ccagcgctga acggtagatg agcaccatga agggattcgt
      181 gctctgcgcc gacgattttg gcctgacggc cgggatcagc catggcatcc tggagctgct
      241 cgccgatggc cggctgagcg ccaccggggc gatgaccaac aggccgcatt ggccggtttt
      301 tgctccggct ctgcgggcct ttgacggtcg cgccgatctc ggcgtgcatc tcaacctgac
      361 ctgcggcgcc ccgctcaccg cgatggcgga tttcgcgccg gaacggcgat tgccgggcct
      421 gccgcgcatc ctgtcgcagg cgatgctgcg ccgcctgccg gaacacgaaa ttgcccatga
      481 actgacggcg cagctcgatg ccttcgagct tgccatgggc cgcgcgcccg atttcatcga
      541 cggtcaccag catgtccacg gcctgccggg catccagccg attttcctgt cggtgatcca
      601 gcggcgctat acggtcggcc accggccgta tgtgcgcgtc tgcggcgacc gggtctggcg
      661 cattctcagg cgcggcgcct ttgccgcaaa ggcgctgaac gtccgccagt tgacgcgcgg
      721 cctcgaccgg cgccttgcca atctcggctt tgccaccaac gatggctttg ccggcttttc
      781 cgatttcaac cccgatggcg attatgcacg cgcctttgca agctaccttg cggcacccgg
      841 cgcgcgccat ctggtcatgt gtcatccagg gtttgtcgat gaggaattgc cgacgctcga
      901 tcctgtgctg cattcgcgcg agcgtgaact cgaattcctc gcttcgaacg ttttcgccga
      961 tctttgccac gctgccggca tggctccggt gcggatggca agggccggcg ccgctctggc
     1021 aggatgagtg cccgggccga ggggccgctc ttgttgcctg catgagaccg ggtataacca
     1081 cccttatgcg tattcctgcc cgtcatacct ggctggtgat gccagccatt ctcgcgctgc
     1141 tgatgacctt cgtcgtctcc ggcatttcaa ccctgcgcgc catcggccct ggcccggagt
     1201 tcctggccaa gtggatgggc tcctgggcct tgtcctatgt ggtggcatat ccgacgctgc
     1261 tggtggtgat gccggtcgtt cgccgcatcc tggcggcgat catggagccc gcgccgaaag
     1321 gctgaaggca gccggactta cgaaacgctg ggtttttgcc tcagcagtcc cggatgcggt
     1381 ggcaggtgac gggccatcgc cggagcagcc cggatgtcgc gtggcggact ggcaggagcg
     1441 ggggccctga cagtctgtcg ccagttgtgt ggattgacgc cccacaggaa cccggtgaag
     1501 aaccacagat ggcgccagcg ctctgcctca tgcaacaggg cgcaggcaaa aatgccgagc
     1561 caggacaggt agatcagcac catgaggctg tcgccggtct gccggtagtt gcggatcgtc
     1621 aggatcactc cgaaatagac gaccgcggtc caggcaaacc cggccagcca gccgtaattg
     1681 acaaaggaac tgagccagaa attgtggatc ggctcgggga agatcgcctc gaattgcagc
     1741 acgcccatgc caatcggatc attgatcgta agattgaacg ccttcagata gcggttgagg
     1801 cgccccattt cgccgacgtc gtaatccttg gcgagcgttg cgcgatcact cagcttttcg
     1861 gcaaattcgg gaataacggc caccgcgatg gctccggcaa tcacggcaac ggccgcaaca
     1921 gccgccaggt tcatgatggt cttggcaagg cgcttgcggt tgagaaacag cgtcaggacc
     1981 aggccggtca gcagggtcgc cgcgacggcg acgcgcgaga acgccaccat cagcccgatt
     2041 gtcaaaaggg cgagtgcggc gatgtagcgc ctgcgggtct ttgtcgtcag aatgaagtag
     2101 atgcagccca gaaccgccgg cggcaggaag gcggcataca tgttgggatc gtcgaagaag
     2161 cccttgcccc gcccgtccca gatgagttcg tcgatcttca gcacaaagcc gatcgtggcg
     2221 aatgttgccg ccaggcaggc cgagccgatc cagaatttgt aaagccgttc gatctgctgg
     2281 cgtccccagt gcatgttgac caggcaggcc gtgatgccga tcgacatcgc atagctggtc
     2341 ttggtcagtt gatagaccac ctcgccatcg taggtgaagg gcatggacga ggtcatcaga
     2401 ccggcccacc aggcgaggat gagagcgagg aaaaccaatc cgcgccgcgt cagcctctgg
     2461 ccgcacagcg gagtcatcag caaggccagg gcgaaagcga tatcgaccgg cgaagggcgc
     2521 gagatcgtaa agttgacgag cacgacggcc aacaggaaaa tgccggtgcg cagccggtcc
     2581 atctgctcgc tgaaggccgt cctgaccatg atcctttcac ctctcctctg cccgttcccg
     2641 caagcggaaa tggcaagcga atcgggcccg ggccgatcct gatcgacgac cctaggagaa
     2701 gttcgttaag ctctcgctaa ccataagggc aaggagcgga ggctggcaca ttctcaagcg
     2761 aagcggagtc cacttcgctt gagaataccc tggtcgggcg cttatttcat cgccgcctgg
     2821 aacatctcgt ccacatagtc ccagttgacg aggtgatcga caaaggcctt cagatagtcg
     2881 ggccggcggt tgcggtagtc gatgtagtag gaatgctccc acacatcgca gccgaggatc
     2941 ggcgcggcgc catgaaccag cgggctttct ccattgggcg atttcatcac cacgatcttg
     3001 ccgtccttga ccgccagcca ggtccagcct gagccgaact gggtgatgcc gccctggatg
     3061 aaatcctcct tcatcttggc gaccgagcca aagccgtcga taaccgcttt ttccagcctg
     3121 cccggcagct tgtcgccgcc gccgttcggc ttcatccagc gccagaagtg caggtgattg
     3181 tagtgctggc cagcattatt gaacaggccg gcattcttgc cgaacgagcc cttcacgatg
     3241 tcttcgaggc tcttgccttc ccattccgag cccttgagca ggttgttgcc gttgttcaca
     3301 taggccaggt gatgcttgtc gtggtgatac tccagcgtct cccgcgacat gtagggttgc
     3361 agtgcatcat gggcataggg caggtcgggc agggtgaagg acatgggctt tcctcgcgct
     3421 cgttgacaaa aaagacatgg ggtcgcggcg gaccttgctg gcaacgtagg gattgatcgg
     3481 ccgtccgaca accggggcga gcggcaattt cgcttcgtca caacaatgtc agggcaggaa
     3541 atattcggca gcgcggacgg ttttcttggc actttctttg caattttgcg gcgccatggg
     3601 cgaatcagcc gcaacgtcat gttgcgcgct tgcgattcgg ggaggctctg atggcccatc
     3661 tcgtccttgg tgtcggcggt ttcatggtgc ttgccggcct gatcgcgctg gtggttggca
     3721 ttgacatgtt gcccaccgag cggggcgttg cggcggtcat cgcctcggtg atggcattgt
     3781 cggccggcgt gatcaccatg gcgctcggcg tcgcgatc
//
LOCUS       DAOMXG010000206         3773 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_13326_length_3773_cov_10.086068, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000206 DAOMXG010000000
VERSION     DAOMXG010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13326_length_3773_cov_10.086068"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1047)
                     /locus_tag="PLQ11_11330"
     CDS             complement(<1..1047)
                     /locus_tag="PLQ11_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRK25534.1"
                     /translation="MAQYIEIKAANPDSLLFYRMGDFYELFFTDAEIASQTLGIVLTK
                     RGKHLGEDIPMCGVPVGRADEYLQRLIAAGHRVAVCEQLEDPAEARKRGSRSVVRRDV
                     TRLVTPGTLTEEALLDPGRANLLVSLARVRSGEAGWSYGLAAVDISTGRFTLIEASEV
                     TVAGTLARLEPSELLLPDAIREDTAAQSVWRDLRCAVAPVPREGFDAVSADERLRRYF
                     GVATLEAFGRFSRAELAAAGAVLAYVDRTQLGTRPPLMPPRREGADALMQIDAATRAN
                     LELMRTLGGERTGSLLATIDRTVTPAGGRLLAERLAGPLTDRAALGDRHAAVAYLVGA
                     GNLGADLRRILRTAP"
     gene            1284..3575
                     /locus_tag="PLQ11_11335"
     CDS             1284..3575
                     /locus_tag="PLQ11_11335"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HRK25535.1"
                     /translation="MSELDSSHRSKRPTITDQEALDFHARGKPGKLEIVPTKPMATQR
                     DLALAYSPGVAVPVKAIAKEPSTAFDYTARGNVVAVISNGTAILGLGNLGALASKPVM
                     EGKAVLFKRFADIDSIDLEVDTEDPDTFINAVRYLGPSFGGINLEDIKAPECFIIEQR
                     LREVMDIPVFHDDQHGTAIIAAAGLINALKITGRDVKSTRLVVNGAGAAGVACVELLK
                     AMGFAPNNVILCDTKGTIYKGRTAGMNQWKSAHAAETKARTLAEALKGADAFFGLSAK
                     GALTADMVASMASNPIIFAMANPDPEILPEEVAAIRDDAIMATGRSDYPNQVNNVLGF
                     PYIFRGALDVRATTINMEMKIAATMALANLAREDVPDEVAAAYGGVRPRFGRDYIIPV
                     PFDPRLMAKIPAAVARAAIDTGVARKPLADLAKYEASLSARRDPVAAVISRVFERVKR
                     NQRRVVFAEGEEEPVIRAAVSFVNQGLGKAILVGREERILATAQNAGVDIDRDGIEIR
                     SAAKSGSRNAVYSQFLYARLQRRGYLQRDCQRLINQDRNHYAASMVALGDADALVTGL
                     TRNYSQTLADIRRVIDAKPGHRVIGVSMVLARGRMVMVADTAVHDMPTSIELADIAVE
                     AAGFAKRLGYVPRVALLAFSTFGYPESERSSRVQEAVQILDKRMVDFEYDGEMGADVA
                     LNPEVMAAFPFCRLSGPANILVMPAFHSAAISTKMLQELGGSTVIGPMLVGLDKPVQI
                     VSINSKDSDIVNMAALAAAGIGG"
     gene            3613..3738
                     /locus_tag="PLQ11_11340"
     CDS             3613..3738
                     /locus_tag="PLQ11_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25536.1"
                     /translation="MTAHPFADPLPIDAVLGSIADALAVRTALDLVAPAGVLRRN"
BASE COUNT          666 a         1224 c         1222 g          661 t
ORIGIN      
        1 gggtgcggtg cgcaagatcc ggcgcaggtc agcgccgagg ttgcccgcgc cgaccagata
       61 ggcaaccgca gcgtgccggt cgcccagcgc cgcccggtcg gtcaaaggtc cggccagccg
      121 ctcggccaga aggcgcccac cggcaggggt cacagtccgg tcaatggtag cgaggagact
      181 gcctgtccgc tcgcccccga gcgtgcgcat cagttcgaga ttggcgcggg tcgctgcatc
      241 gatctgcatc agcgcatcgg cgccctcgcg gcgcggcggc atcagcggcg ggcgggtgcc
      301 aagctgcgtg cgatcgacat aagcgaggac ggcgccggcg gctgccagtt cggcacggga
      361 gaagcggccg aaggcttcca gcgttgccac gccgaaatag cgcctgagcc gttcatcggc
      421 cgagaccgca tcgaatcctt cacgcggaac gggcgcaacc gcacatcgga gatcccgcca
      481 gacggactgg gccgccgtat cctcgcggat ggcatccggc agcagcaatt cgctgggttc
      541 cagccgcgcc agcgttcccg caacagtcac ctcgctcgcc tcgatcaggg tgaaccggcc
      601 ggtggaaatg tcgaccgctg cgagcccata gctccagccg gcctccccgg aacggacgcg
      661 ggccagcgaa accagcaggt tggcacggcc gggatcgagc agcgcctcct cggtgagggt
      721 gccgggggta acaagccggg tcacgtcgcg gcgcaccacg gatctggagc cgcgcttgcg
      781 ggcctcggcc ggatcctcga gctgttcaca gaccgcgacg cgatggccgg ctgcaatcag
      841 gcgctgcaga tactcgtcgg cgcggcccac cggaacgccg cacatcggga tatcctcgcc
      901 caggtgcttg ccccgcttgg tcagcacgat gcccagcgtc tggctggcga tctcggcatc
      961 ggtgaagaac aattcgtaga agtcgcccat tcgatagaac aacaagctgt ccggattggc
     1021 ggccttgatc tcgatatact gggccatcac cggagtgggg cgcgcatcgg ccgctgcgac
     1081 gagacggccc gggccgggca cataggcttg ttgcgggata tcaggcgaca ggtcgttcat
     1141 ggccctgttc tacccacggt ttgctcttta ggccaccgct tccgctttgc cggcgcaaaa
     1201 cgcgcacact tttcctggca ggcgacttgt tattgccggc cgctgttctt agtgtcgaac
     1261 ccttcatgcc gcaggattgt cccatgagtg aactcgattc cagccatcgc tccaagcgcc
     1321 ccaccatcac cgatcaggag gcgctggatt tccatgcccg cggcaaaccg ggcaagctgg
     1381 agatcgtacc gaccaagccg atggcgaccc agcgggatct ggcgctggcc tattcgccgg
     1441 gcgtcgcagt tcccgtcaag gcgatcgcca aggaaccctc aacagccttc gactataccg
     1501 cacggggcaa tgtcgttgcg gtgatctcca acggcacggc tattctgggc ctgggcaacc
     1561 tcggggctct cgcgtccaag ccggtgatgg aaggcaaggc ggtgctgttc aagcgcttcg
     1621 ccgatatcga ttccatcgac cttgaggtcg acaccgagga cccggatacg ttcatcaacg
     1681 cggtgcgtta cctcggcccc tcgttcggag gcatcaatct cgaggatatc aaggcgccgg
     1741 aatgcttcat catcgagcag cggctgcgcg aggtcatgga cattccggtg ttccatgacg
     1801 accagcacgg cactgcgatc atcgccgccg ccggcctcat caatgctctg aagatcaccg
     1861 ggcgggacgt caagtccacg cggctggtgg tcaacggcgc cggcgcggcg ggggttgcct
     1921 gcgtcgaact gctcaaggcg atggggtttg cccccaacaa cgtcatcctg tgcgatacca
     1981 agggcaccat ctacaagggg cgcaccgccg gcatgaacca gtggaagagc gctcatgccg
     2041 ccgaaaccaa ggcgcgcacg ctggccgagg cattgaaggg cgctgatgcg ttcttcggcc
     2101 tctccgccaa gggcgcgctg actgcggaca tggtcgccag catggcgtcc aatccgatca
     2161 tctttgccat ggccaacccg gacccggaaa tcctgccgga agaagtcgct gccatccgcg
     2221 acgatgcgat catggccacc gggcgttcgg actaccccaa tcaggtcaac aacgtgcttg
     2281 gctttcccta catcttccgg ggggcgctcg atgtgcgcgc caccaccatc aacatggaga
     2341 tgaagatcgc cgccacgatg gcgctggcaa atcttgcccg cgaagatgtt cccgatgagg
     2401 ttgccgccgc ctatggcggc gtgcgcccgc ggtttggccg cgactatatc attccggtgc
     2461 cgttcgatcc gcggctgatg gccaaaattc cagccgctgt tgcccgtgcg gcgatcgata
     2521 cgggtgtcgc ccgcaagccg cttgctgatc ttgccaaata cgaagcttcg ctgtcggcac
     2581 ggcgcgaccc ggtggccgcc gtgatctcgc gggtgttcga gcgggtcaag cgcaaccagc
     2641 gccgggtcgt ctttgccgag ggcgaggagg agccggtgat ccgcgccgcg gtgtccttcg
     2701 tcaatcaggg gctgggcaag gcgattctcg ttggccggga agaacgcatc ctggcaaccg
     2761 cccagaatgc cggtgtcgat atcgatcgtg acggcatcga gatccgttca gcggccaaat
     2821 ccggctcgcg caatgcagtc tattcgcagt tcctttatgc ccgcctgcag cgcaggggct
     2881 atctgcagcg cgactgccag cgcctgatca atcaggatcg caaccattat gccgcctcga
     2941 tggtggctct gggcgatgcc gatgcgctgg ttacagggct gacccgtaac tattcacaga
     3001 cgttggcgga tatccgccgc gtcatcgatg ccaagcccgg acaccgcgtc atcggcgtgt
     3061 cgatggtgct ggcgcgcggc cgcatggtca tggttgccga tacggcggtg catgacatgc
     3121 cgacctcgat tgaactggcc gacatagcgg tggaggccgc cggatttgcc aagcggctcg
     3181 gctatgtgcc gcgcgtggcg ctgctggcgt tctcgacctt cggctatccg gagagcgagc
     3241 gctccagccg ggtgcaggag gcggtgcaga ttctcgacaa gcggatggtc gacttcgagt
     3301 atgatggcga gatgggagcc gatgtggcgc tcaatcccga ggtgatggcg gcattcccgt
     3361 tctgccgcct gtcgggaccg gccaacatcc tggtgatgcc ggcattccat tcggcagcga
     3421 tctcgaccaa gatgctgcag gagctgggcg gctcgaccgt catcggcccg atgctggttg
     3481 gtctcgacaa gccggtgcag atcgtttcga tcaactcaaa ggattccgac atcgtcaaca
     3541 tggcggcact ggcagcggcc ggcatcgggg gttaacccgt aggccgaccc gccgcagcgt
     3601 ccgggaccgc gcatgaccgc ccatcccttc gctgatcccc tgccgatcga cgccgtgctg
     3661 ggcagcatcg cggacgctct ggccgtgcgc acggcgcttg atcttgtcgc gccagcgggt
     3721 gtgctcaggc gcaactgaag atcagcatgg ccgacaaaga aatcgtcaag ctt
//
LOCUS       DAOMXG010000207         3738 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_13481_length_3738_cov_8.354059, whole genome shotgun sequence.
ACCESSION   DAOMXG010000207 DAOMXG010000000
VERSION     DAOMXG010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13481_length_3738_cov_8.354059"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..1314)
                     /locus_tag="PLQ11_11345"
     CDS             complement(124..1314)
                     /locus_tag="PLQ11_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HRK25537.1"
                     /translation="MISRRGLMAAAPLAAVSGPTMAQQPAPALRGTGDLGVIIERANG
                     SVLIVETTGRSILKRVTGLGDLSHASISFSRDERFAYLFGRDGGLTKIDILEGRIAAR
                     IVQGGNSIGGAISDDGRLIAVSNYEPGGVKVFDAETLALVADVPAVSAAAPKSKTVGI
                     VDVPGRAFVYTLYDAGEIWKLELPEGAEPQLTKLTGIGKLPYDANVTSDGRHYFAGLF
                     GEDGVVHVDLWQEPLRAKRVLANYGRGQEPLPVYKMPHFEGWGATQNKLFLPAVGRDE
                     LLVIDLATLQEIGRVKTHGQPVFAVARPDGRHIWVNYAHPKNNVVEVVDSETLAVVRR
                     LEPGPAVLHLEFTPRGHEAWVSVRDTDTVIVYNTRTFAEKARLAAQKPSGIFFTARAH
                     VIGK"
     gene            complement(1311..1586)
                     /locus_tag="PLQ11_11350"
     CDS             complement(1311..1586)
                     /locus_tag="PLQ11_11350"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRK25538.1"
                     /translation="MLASAAASAEPDRERLKHLLVQECGSCHGLTLNGGLGRPLTREA
                     LANFPVETVRDIILDGLPGTPMPPWRPLLSIPEASVIADMLKSGEIR"
     gene            complement(1552..2373)
                     /gene="cobA"
                     /locus_tag="PLQ11_11355"
     CDS             complement(1552..2373)
                     /gene="cobA"
                     /locus_tag="PLQ11_11355"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HRK25539.1"
                     /translation="MAIGHVALVGAGPGDPELLTMKAVKALAQAEVVVHDRLVSDAVL
                     KVAPDSAMRISVGKEAGRHSYKQEEINDLLVHLALQGKNIVRLKGGDPFVFGRACEEI
                     EALEAHGITWSVVPGITAAQGCASSARVPLTHRGLARSLRFVTGHCRADQPLDLDWKG
                     LADPQTTVVFYMGHATAPQLSAKLIENGLPAETPTLCISQGTTPQERRMFTRLDRLPV
                     DLERAHLPSPILIIVGNAVGWRPGQSTGDIGDETGLPSCEPDSAGAGQRRRFGRT"
     gene            complement(2375..2782)
                     /locus_tag="PLQ11_11360"
     CDS             complement(2375..2782)
                     /locus_tag="PLQ11_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014283745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HRK25540.1"
                     /translation="MRRLFVPVLISLAAPVLAQPADDTAERCAEAAARYKDLYGRDHT
                     AEPVRTVLMFKYRFCPEKVTVRAGEKLRYINVDKRTSHSVWLKQAGQPESERVFYEGV
                     IEVDTRLPNGDHQVLCGPHWETDRMIGTISVEK"
     gene            complement(2793..>3738)
                     /locus_tag="PLQ11_11365"
     CDS             complement(2793..>3738)
                     /locus_tag="PLQ11_11365"
                     /inference="COORDINATES: protein motif:HMM:NF021010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HRK25541.1"
                     /translation="GGKINWANVPARTIGFFYPGQASYEWVVTGKDHGGARAFLRGGD
                     RCVTCHDKEVRDMGAKIVSGSKAEATPIKGKRGSVDVQVQAAHDKEKVYFRFQFPKAP
                     HTPMDGVDGGKMDAANETKLSVMFSGAGVEKADAAGCWVTCHHDARNMPDAPKPDALK
                     AFPADGGLDLAAGVTKYLNESRASIELRDSPRGGWDKLKGKDDLAKLAGEGKVMELLR
                     YRSGGAPEHGLVGAQRVMTGLSPVEASGGLDGDTWTVVIARPLKGAAPGEISFDPGKK
                     YVVSFALHEDFTASRFHHVSLAYEFSVDGEGEVVAKAQ"
BASE COUNT          641 a         1235 c         1206 g          656 t
ORIGIN      
        1 aacccacgcg ctcgccgacc tcggcgaacg ggcgcgcagt caacggaaaa tcgcgctgga
       61 atccatccac cagcgcccgt gtcgccgcgt cgagatcgcc gcagggaagc acgaccgcca
      121 tggctatttc ccgatcacat gggcgcgggc ggtgaagaag atgccggatg gcttctgcgc
      181 cgcaagccgc gccttttcgg caaaggtccg ggtattgtag acgatcaccg tatcggtatc
      241 gcgcaccgac acccacgcct cgtggccgcg cggggtgaat tcgagatgca gcaccgccgg
      301 ccccggctcc aacctgcgga cgacggcaag cgtctcgctg tccaccacct cgaccacgtt
      361 gttcttgggg tgggcataat tgacccagat gtgccggcca tccggccgtg ccaccgcgaa
      421 caccggctgg ccatgcgttt tcacccggcc gatttcctgc aatgtggcaa ggtcgatcac
      481 cagcagttcg tcacgcccga ccgccggcaa aaacagcttg ttctgcgtgg cgccccaacc
      541 ctcgaaatgc ggcatcttgt agaccggcag cggctcctgc ccgcggccat aattggccag
      601 cacgcgcttt gccctgagcg gctcctgcca caggtcgaca tgcaccacgc catcctcgcc
      661 gaacaggccg gcgaagtaat gccggccatc cgaggtgaca ttggcgtcat agggcagttt
      721 gccgataccg gtcagcttgg tcagctgcgg ttctgcccct tcgggcaatt caagcttcca
      781 gatctcgccg gcatcataga gcgtgtagac gaaggcgcgg cccggcacat cgacaatgcc
      841 gacggttttc gatttcggtg cggcggcgga aaccgccggc acatcggcaa ccagcgccag
      901 cgtctcggca tcgaacacct tgacgccgcc gggctcgtaa ttggaaacgg cgatcagccg
      961 gccatcgtcg gaaatcgccc cgccgatgga attgccgccc tgcacgatgc gcgcggcgat
     1021 gcggccttcg agaatgtcga tcttggtcaa cccgccatcg cggccgaaca gataggcaaa
     1081 gcgctcatcg cgtgaaaagc tgatcgaggc atgcgacagg tcgccgagcc cggtgacgcg
     1141 cttgagaatg gaacggccgg ttgtctcgac gatcagcacg ctgccgttgg cgcgttcgat
     1201 gatgacgccg agatcgccgg tgccgcgcag ggcaggggcg ggctgctggg ccatggtggg
     1261 cccggacacg gcggcaaggg gggcagcggc catcaggccg cgacggctga tcatctgatc
     1321 tctccgcttt tcagcatgtc ggcgatgaca ctggcctcgg ggatgctgag caatggccgc
     1381 cacggcggca tcggcgttcc gggcaggccg tcaaggatga tgtcgcgcac ggtctcgacc
     1441 gggaaattgg ccagcgcctc acgggtcagc ggccggccca gcccgccgtt gagcgtcagc
     1501 ccgtggcagg agccgcattc ctggacaagc aggtgcttca ggcgctcccg gtcaggttcg
     1561 gccgaagcgg cggcgctggc cagcaccagc actgtcaggc tcgcaggaag gaaggccggt
     1621 ttcgtcacca atgtcacccg tgctctggcc tggacgccag ccaaccgcat tgccgacgat
     1681 gatcaggatg gggctgggaa gatgggcccg ctccagatcg accggcaggc ggtcgaggcg
     1741 ggtgaacatt cgccgttcct gcggtgtcgt gccttgacta atgcaaagcg tcggcgtttc
     1801 cgcgggcagg ccgttttcga tcagctttgc tgacaattgc ggcgctgtcg cgtgtcccat
     1861 gtagaaaaca acggtggttt gcgggtccgc cagccccttc cagtccagat cgagcggctg
     1921 gtcggcgcgg caatgtcccg tgacaaatct caaggaccgg gcaaggccgc gatgggtcag
     1981 gggaacacgc gcggacgagg cgcatccctg cgcggcggtg atgcccggca ccaccgacca
     2041 ggtgattcca tgggcttcca gcgcctcgat ctcctcgcag gcgcggccga acacgaacgg
     2101 atcaccaccc ttgagccgga caatgttctt gccctgtagt gcgaggtgga ccagcaggtc
     2161 gttgatctct tcctgcttgt aggaatggcg gccggcttcc ttgccgaccg agatgcgcat
     2221 ggcgctgtcc ggggcgactt tcagcacggc atccgagacc agccgatcgt gcaccaccac
     2281 ttcggcctgc gccagcgcct tgacggcctt catcgtcagc agttcggggt cgcccggtcc
     2341 ggcaccgaca agcgccacat gtccgattgc catgttactt ctccacgctg atggtgccga
     2401 tcatccggtc ggtttcccaa tgcgggccgc acagaacctg atggtcgcca ttgggcaggc
     2461 gggtatcgac ctcgatcacg ccttcgtaaa agacgcgctc ggattccggc tggccggcct
     2521 gcttcagcca caccgaatgc gaggtacgct tgtcgacgtt gatgtaacgc aacttctcgc
     2581 cggccctgac cgtcaccttt tccgggcaga accggtattt gaacatcagg acggtcctga
     2641 ccggttcggc ggtatggtcg cggccgtaga ggtccttgta gcgggcggcc gcctcggcac
     2701 agcgttcggc ggtatcgtcg gccggctggg ccagaaccgg cgctgccagt gagatcaaca
     2761 caggaacgaa aagccgccgc atcgctctgc cctcactgcg ccttggcgac cacctcgccc
     2821 tcgccgtcga ccgagaattc gtaagccagc gagacgtggt ggaagcgcga ggcggtgaag
     2881 tcctcgtgca gcgcgaagga gacaacgtat ttcttgcccg gatcgaagct gatctcgccg
     2941 ggggctgccc ccttgagcgg gcgggcgatg accacggtcc aggtatcgcc atcgagccca
     3001 cccgaggctt caaccggcga aagtccggtc atgacgcgct gggcgccgac aagaccatgt
     3061 tccggcgctc cgcccgagcg gtagcgcaac agttccatga ccttgccttc gccggcgagc
     3121 ttggcgaggt cgtccttgcc cttgagcttg tcccagccgc cgcgcgggga atcacgcagc
     3181 tcgatcgagg cgcgcgattc gttgagatat ttggtgacgc ctgccgccag atcgaggccg
     3241 ccgtcggccg ggaaggcctt gagagcgtcc ggcttgggcg catccggcat gttgcgggcg
     3301 tcgtggtggc aggtcaccca gcagccggcc gcatcggcct tctcgacacc ggcgccggag
     3361 aacatcaccg acagcttggt ctcgttggct gcgtccatct tgccgccatc gacgccgtcc
     3421 atcggcgtat gcggcgcctt tgggaactgg aagcggaagt agaccttctc cttgtcatgg
     3481 gcggcctgaa cctgcacatc gaccgagcca cgcttgccct tgatcggcgt cgcctcggcc
     3541 ttcgagccgg agacgatctt ggcgcccatg tcgcgcactt ccttgtcatg gcaggtgacg
     3601 cagcggtcgc cgccgcgcag gaaggcgcgg gctccgccat ggtccttgcc ggtgaccacc
     3661 cattcgtacg acgcctggcc gggatagaag aacccgattg tcctggccgg cacgttcgcc
     3721 cagttgatct tgccgccg
//
LOCUS       DAOMXG010000208         3663 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_13865_length_3663_cov_10.921286, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000208 DAOMXG010000000
VERSION     DAOMXG010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13865_length_3663_cov_10.921286"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1420)
                     /locus_tag="PLQ11_11370"
     CDS             complement(<1..1420)
                     /locus_tag="PLQ11_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018324895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRK25542.1"
                     /translation="MELFDNLWLGLQTSMTPINLLYALMGVFLGTAIGVLPGLGPAAT
                     IAMLLPATFAMPPVTALIMLAGIFYGAQYGGSTTAILVNLPGETSSVVTALDGYQMAR
                     QGKAGRALATAALSSFFAGTVCTLLIALFAPPLAEVALKFGPAEYFSLMVLGLIASIV
                     LAHGSILHALGMIILGLLLGLVGTDVNSGAQRYTFGLPELADGIGFVIVAMGMFGIGE
                     IIANLEHESTRDVSIKKVEGLWPTKEDFKRIVAPTLRGTALGSLLGILPGGGAALAAF
                     GAYALEKKVSKNKHEFGKGAIEGVAAPESANNAGAQMSFIPMLTLGIPANPVMALMIG
                     AMIIQGIQPGPSVMTEQPKLFWGLIVSMWIGNLMLLVLNLPLIGMWVRMITIPYRYLF
                     PAIIVFCAIGVFSLNNANFDVYLMALFGVIGYIFKKLDCEPAPLLLGFILGPMMEEFL
                     RRALLLSKGDPMVLVTRPISATM"
     gene            complement(1434..1916)
                     /locus_tag="PLQ11_11375"
     CDS             complement(1434..1916)
                     /locus_tag="PLQ11_11375"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HRK25543.1"
                     /translation="MKIRSEKDFWAGLLYVLLALGFLWFGREYKMGVASRMGPGYFPL
                     TLGWILFGFGAISIFRSFSTDGPPVGGIAWKKLTIITVGVLVFGFLVGKAGLVFALPA
                     LVVISALASEQSEFDLKALLVLIGLTIFCIVVFVKGLGVPMPIFGAWFDGIVPPSWQR
                     "
     gene            complement(2059..2946)
                     /locus_tag="PLQ11_11380"
     CDS             complement(2059..2946)
                     /locus_tag="PLQ11_11380"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRK25544.1"
                     /translation="MSSSGGDPIWSWPEDRWRSLIESVRAGRSLKPRKWKNGARAAVA
                     IAVHFDNEIGMLAAGMTTPARLSQGHYGARAGLGRVLGALKRHSVPATFFVPAVAAML
                     YGDELRTIAGAGHEIALAGWIGEVPAQLPPGVERDLITGARETIARLVGQAPTGYQAP
                     GSGLSAETPAVLCDLGFIYESSLMSDDDPFDLTMAGQPTGLVSLPAAAERDDATYWGA
                     GDTAGTTSPHAVFEILRRDLERAYDEGGLFQLTLHPALIGRRSRIWVLEELFGLARSL
                     PGVWFATHHEIAHWCRSKA"
     gene            3223..>3663
                     /locus_tag="PLQ11_11385"
     CDS             3223..>3663
                     /locus_tag="PLQ11_11385"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRK25545.1"
                     /translation="MRHQDNVQASFLLIAALALFTFETLCVRLLNGVATPPQAVLFRS
                     LAQLVVVGTWMLWRRQLPLLRSPHLKLHLARGIVSIVSWYLYYRSFQKLEFSLATLLS
                     FASSLFVVLLAGPVLGERAHPVSWLATLAGFGGIALAAGAGGGAV"
BASE COUNT          735 a         1191 c         1107 g          630 t
ORIGIN      
        1 gcatggtggc actgatcggc cgggtgacca gcaccatcgg atcgcccttg gacaagagca
       61 gggcgcggcg caggaactcc tccatcatcg ggcccagaat gaagccgagc agcagcggtg
      121 ccggctcgca atcgagcttc ttgaagatgt aaccgatgac gccgaacagc gccatcagat
      181 agacgtcgaa gttggcgttg ttgaggctga aaacgccgat ggcgcagaac acgatgatcg
      241 ccgggaacag atagcgatag gggatggtga tcatccgcac ccacatgccg atcagcggca
      301 ggttgagtac cagcagcatc aggttgccga tccacatcga gacaatcaga ccccagaaca
      361 gctttggttg ctcggtcatc accgaggggc ctggctggat gccctggatg atcattgcgc
      421 cgatcatcag cgccatcacc ggattggccg ggatgccgag ggtgagcatc gggatgaacg
      481 acatctgcgc gccggcattg ttggccgatt cgggcgcagc cacgccctcg atcgcaccct
      541 tgccgaactc gtgcttgttc tttgaaacct tcttttccag tgcataggcg ccaaaggccg
      601 ccagcgccgc gccaccgccc ggcaggattc caagcaggga accgagcgcc gtgccgcgca
      661 gggtcggcgc cacgatgcgc ttgaagtcct ctttcgtcgg ccacaagcct tcaaccttct
      721 tgatcgagac gtcgcgggtc gattcatgct cgagattggc gatgatctcg ccgatgccga
      781 acatgcccat ggccacgatg acaaagccga tgccgtcggc cagttcaggc aggccgaagg
      841 tgtagcgctg ggcgccggag ttcacgtccg tgccgacgag cccgagcagc aagccgagga
      901 tgatcatgcc gagcgcatgc aggatcgagc catgcgccag cacgatcgag gcgatgaggc
      961 ccagcaccat cagcgagaaa tattcggccg ggccgaactt cagcgccact tccgccagcg
     1021 gcggcgcgaa cagcgcgatc agcagcgtac agaccgtgcc ggcaaagaac gaggacagcg
     1081 cggcggtggc cagcgcccgg ccggccttgc cctgccgcgc catctggtaa ccgtcgagcg
     1141 cagtgacgac ggaggaggtc tctcccggca gattgaccag aatggccgtt gtcgagccgc
     1201 cgtattgcgc gccataaaaa atgccggcga gcatgatcag cgcggtcacc ggcggcatgg
     1261 cgaaggtcgc cggcagcagc atggcgatgg tcgccgcagg tccaagtccc ggcagcacgc
     1321 cgatcgccgt gccaaggaaa acgcccatca gcgcgtaaag caggttgatg ggagtcatcg
     1381 aggtttgcag gccgagccag aggttgtcga acagttccat ggtcctgctc ccctcagcgc
     1441 tgccacgacg gcggcacgat gccgtcaaac cacgccccga agatcggcat gggaacaccc
     1501 agccctttga cgaagacgac gatgcagaag atggtcaggc cgatcagcac cagcagcgcc
     1561 ttgaggtcga actcggattg ttccgaggca agggccgaga tcaccacaag ggccggcagc
     1621 gcgaacacca gcccggcctt gccaacaaga aagccgaaca ccagcacccc gacagtaatg
     1681 attgtcagct tcttccaggc gatcccgccc acgggagggc cgtccgtcga gaacgagcgg
     1741 aagatcgaga tggcaccaaa tccgaacagg atccagccaa gcgtcagcgg aaaatagccc
     1801 ggccccatcc gagaggccac acccatcttg tactcgcgtc cgaaccacag gaacccgagg
     1861 gcgagcagga catacaacag ccccgcccag aagtcctttt ccgaccggat tttcaccact
     1921 gaattccccc cagagccgtg acttaagtct attgttcctt ggtgttacat cgcgcgttcg
     1981 ggaacgtcta ccgtgcagca aaaaaaagcc gatttgacgg tcatgtctcg caggcaaaga
     2041 ccaagcgccg cttgccgctc atgccttgct acggcaccag tgcgcgatct catggtgcgt
     2101 tgcaaaccac acacccggca aggatctggc caacccgaac agttcctcca gaacccagat
     2161 gcgcgagcgc cggccgatca aggcagggtg cagggtcagc tggaacaggc ctccctcatc
     2221 ataggcccgc tccaggtcac gccgcagtat ctcgaataca gcatgcggtg aggtcgtgcc
     2281 ggccgtgtcg cccgcccccc aatatgtcgc atcgtcgcgt tccgcagcag ccggcagcga
     2341 aaccaaccca gtcggctgcc ccgccatcgt cagatcgaac gggtcatcat cggacatcag
     2401 cgacgattcg tatatgaaac caagatcaca caacacagcc ggtgtttccg ccgagaggcc
     2461 gctgccggga gcctggtagc ccgtcggcgc ctgcccgacg aggcgggcga tggtctcccg
     2521 cgcgccggtg atcagatccc gttccacacc gggtggcagc tgtgccggca cctcgccgat
     2581 ccagccggca agcgcaatct cgtgccctgc tccggctatg gtgcgcagct cgtcaccata
     2641 cagcatggca gcgacagccg gcacgaaaaa ggtcgccggc acactatgcc gcttcagcgc
     2701 gcccagcacg cgccccaacc cggcacgcgc gccataatgg ccctggctga gccgggccgg
     2761 cgttgtcatg ccggcggcga gcatgccgat ctcgttgtcg aaatgaaccg cgatggccac
     2821 ggctgcgcgg gctccgtttt tccatttgcg cggcttcagc gatcggccgg cgcgcacgct
     2881 ttcgatgaga gagcgccagc ggtcctcagg ccaactccag attggatcgc cgccggaact
     2941 gctcatgcca tctctcctga atggcccgcc gtccttgcgg gccgggtaaa aatgccgaat
     3001 gcttagcgtt agatcacgct cacactttga ttgaagcatc aaaccgtcag cgtgatcatt
     3061 ttcaaaagtt tagagcatgc ttgtcgcgat tagccggctt ccacctaatc gcagcatgct
     3121 ctagaaggac tcgctcacct atcctgcgcc agtgtggcat cgggtttcaa gatgtcggag
     3181 acgcccctag cccccggttc accggcgccg cctgctctgc cgatgcgtca tcaggacaat
     3241 gtgcaggcca gctttctgct gatcgcggcc ctggccctgt ttaccttcga gacgctatgc
     3301 gtgcgcctgc tgaatggcgt ggcaacgccg ccccaggcgg tgctgttccg ctcgcttgcc
     3361 cagctggtcg tggttggcac ctggatgctg tggcgccgtc agttaccgct gttgcgcagc
     3421 ccacacctca aactgcacct ggcgcgggga atcgtgtcaa tcgtcagctg gtatctgtat
     3481 taccgaagct ttcagaagct tgagttttcg cttgcaacgc tgctgtcctt cgccagttcg
     3541 ctgtttgttg tgctgctggc aggtccggta ctgggtgagc gcgcgcatcc ggtgtcctgg
     3601 ctcgccacgc ttgccggctt cggcggcatc gcgctcgcgg ccggggcggg aggcggcgct
     3661 gtc
//
LOCUS       DAOMXG010000209         3595 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_14239_length_3595_cov_12.048870, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000209 DAOMXG010000000
VERSION     DAOMXG010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14239_length_3595_cov_12.048870"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..905)
                     /locus_tag="PLQ11_11390"
     CDS             complement(51..905)
                     /locus_tag="PLQ11_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRK25546.1"
                     /translation="MLFQASRSATLAGLGAILLWSLLALLTAATVGIPPFQVMAITFA
                     IGGLTGLAVLAARHRLAELVQPPAAWALGLYGLFCYHAIYFTALKLAPPAEASLVAYL
                     WPLLIVLMSGLLPGGGLSLRHVIAALMGLAGMALLALGKGALTFSPEHRLGYGLALVS
                     AFIWSSYSVLSRRMASVPNGAVVGHCLATAGLAALCHLTIERWVTPTPSGWLALVGLG
                     LGPVGAAFFLWDTGMKLGDLRFLGVASYATPVLSTLVLVATGTTTASLSLALACLLIA
                     GGAFIARR"
     gene            complement(1087..1812)
                     /gene="phbB"
                     /locus_tag="PLQ11_11395"
     CDS             complement(1087..1812)
                     /gene="phbB"
                     /locus_tag="PLQ11_11395"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HRK25547.1"
                     /translation="MARVALVTGGTRGIGAAISLALKEAGYKVAASYAGNDEAAAKFK
                     AETGIPVFKWDVGDFDACAAGIKQVEAEVGPIDVLVNNAGITRDGMFHKMTKANWDAV
                     INTNLNSLFNMTRNVWEGMQARKFGRVIVISSVNGQKGQAGQANYSAAKAGDIGFVKA
                     LAQEGVRSNITVNAICPGYIGTEMVAAIPEEVLKAKILPLIPVGRLGKPEEIGRAVVF
                     LASDDSGFMTGSTLSANGGQYMI"
     gene            complement(1911..3089)
                     /locus_tag="PLQ11_11400"
     CDS             complement(1911..3089)
                     /locus_tag="PLQ11_11400"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HRK25548.1"
                     /translation="MARNEIVIASVARTPVGSFSGAFADMSAHQLGAEAIKAALSRAK
                     VAPNEVDEVIMGQILTAGQGQNPARQAAVAAGIPVEVTAFGINQLCGSGLRAVALGMQ
                     QIANGDADIVVAGGQESMSQSQHTAYLRSGVKMGELKMMDSMLRDGLWDAFQGYHMGT
                     TAENVATKYQISRDEQDKFAVASQNKAEAAQKAGKFKDEICPITVKTRKGDIVVDADE
                     YPRHGATLDGMTKLRPAFSKDGTVTAGNASGINDGAAAAVLMTAAEAKKRGITPMARI
                     VSWATCGVDPAIMGTGPIPASRKALEKAGWKISDLDLVEANEAFAAQAISVNKDLGWN
                     ADIVNVNGGAIAIGHPVGASGARILATLLYEMKRRNAKKGLATLCIGGGMGIAMCVER
                     "
     gene            3356..>3595
                     /locus_tag="PLQ11_11405"
     CDS             3356..>3595
                     /locus_tag="PLQ11_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015341644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HRK25549.1"
                     /translation="MAHDKTAHVIKKYANRRLYHTGTSAYVTLDDLADMVKSGQDFIV
                     NDAKTGEDITHSVLTQIIFEQESKGENLLPVNFLRQ"
BASE COUNT          689 a         1117 c         1163 g          626 t
ORIGIN      
        1 cctgcaactc gccgcagcaa ttctatggat gcgttgaatg tcgattcagc ctagcggcgc
       61 gcgatgaagg cgccgccggc gatcagcaag caggcgagcg ccagcgacag gcttgccgtt
      121 gtcgtacccg tcgctaccag caccagagtc gacaggaccg gcgttgcata ggaggctacg
      181 ccgagaaagc ggagatcgcc cagcttcatg ccggtatccc acaggaaaaa ggccgcaccg
      241 accggcccaa gcccaaggcc gaccagtgcc agccagccgg atggcgtcgg cgtcacccag
      301 cgttcgatgg tcagatggca gagtgcggca aggccagccg ttgccaggca atggcccacg
      361 acggccccgt tcggcaccga ggccatgcgt cgcgacagca ccgaatagga cgaccagatg
      421 aaggcggaga ccagggccag accatagccc agccggtgtt cgggggaaaa cgtcagggcg
      481 cccttgccga gcgccagcag agccatgccg gcgagcccca tcagcgccgc gatgacatgg
      541 cggagggaca ggccgccacc gggcagcagc ccgctcatca gcacaatcag cagcggccag
      601 agataggcaa cgaggctggc ctcggcgggc ggggccagct tgagggcggt gaagtagatg
      661 gcatggtagc agaacagtcc atagagcccg agcgcccagg cagcgggtgg ctggacgagt
      721 tcggcaagcc tgtgacgcgc cgcaagaacg gcaaggccgg tcaggcctcc gatggcgaag
      781 gtgatcgcca tgacctgaaa cggaggaatg cccactgtcg ccgcggtgag aagcgccagc
      841 agactccaca gcaggatggc gccaaggccg gccagggtgg cggaacggga ggcttgaaac
      901 aacatcggcc gcgcatagag catgctgcga ttaagtggaa gccagttaat cacaaaagca
      961 tgctctatcc tattgataaa gatcacgttg tgttttgata attccatcaa aacacaacgt
     1021 gatccggcat gcgcggccgt gtttcgtcac cggcccgagg ccgggtattg atccgcttcc
     1081 tgacgatcag atcatgtact ggccgccgtt ggccgagagc gtcgaaccgg tcatgaagcc
     1141 cgaatcgtcg gaggcgagga agacgaccgc gcggccgatt tcctccggct tgccgagacg
     1201 gccgactgga atcagcggca ggatcttggc cttcagcacc tcttccggaa tcgccgccac
     1261 catctcggtg ccgatatagc cggggcagat tgcgttaacg gtgatgttcg agcgcacacc
     1321 ttcctgggcc agcgccttga cgaagccgat gtcaccggcc ttggcggcgg aataattggc
     1381 ctggccggcc tggcccttct ggccgttgac cgaggaaatg acgatgacgc ggccgaactt
     1441 gcgggcctgc atgccttccc acacgttgcg ggtcatgttg aacagagaat tcaggttggt
     1501 gttgatcacc gcatcccagt tggccttggt catcttgtgg aacatgccgt cgcgggtgat
     1561 gccggcgttg ttgaccagca cgtcgatcgg gccaacctcg gcctcaacct gcttgatgcc
     1621 ggcggcgcag gcgtcgaaat cacccacgtc ccacttgaac acagggatgc cggtctcggc
     1681 cttgaacttg gcggcggcct catcattgcc ggcgtagctc gccgcaacct tgtagcctgc
     1741 ctccttcaga gccagcgaaa tcgccgcgcc gatgccgcgg gtgccgccgg taaccaatgc
     1801 aactcttgcc ataatcgttt cctctccaga ctgaacgggg gccggccgca gttaagcacg
     1861 gtccggcaag acgcccgggc ggtggggccg cccgggcttg cagggtgatg tcagcgctcg
     1921 acgcacatgg cgatgcccat gccgccgccg atgcaaagcg tggccaggcc cttcttggcg
     1981 ttgcggcgct tcatttcgta aagcagcgtc gccaggatgc gggcgcccga ggcgccgacc
     2041 gggtggccga tggcgatggc gccgccgttg acattgacga tgtcggcgtt ccagccgaga
     2101 tccttgttga ccgaaatcgc ctgcgcggca aaggcctcgt tggcctcgac cagatcgaga
     2161 tcggagatct tccagccggc cttctcgagc gccttgcgcg aggccgggat cgggccggtg
     2221 cccatgatcg ccggatcgac gccgcaggtt gcccatgaga cgatgcgggc catcggcgtg
     2281 ataccgcgct tcttggcctc ggcggcggtc atcagcaccg cggcggcggc gccgtcattg
     2341 atgccagagg cgttgccggc ggtcaccgtg ccgtccttgg agaaggccgg acgcagcttg
     2401 gtcatgccat cgagcgtggc gccgtggcgt ggatactcgt cggcgtcgac gacgatatcg
     2461 cccttgcggg tcttgaccgt gatggggcag atctcgtcct tgaacttgcc ggccttctgg
     2521 gctgcctcgg ccttgttctg ggaggcaacg gcgaacttgt cctgctcgtc gcggctgatc
     2581 tggtacttgg tggcgacgtt ctcggccgtg gtgcccatat ggtagccctg gaaggcatcc
     2641 cacaggccgt cgcgcagcat cgaatccatc atcttgagct cgcccatctt cacgcccgag
     2701 cgcagatagg cggtgtgctg ggattggctc atcgattcct gaccgccggc cacgacgatg
     2761 tccgcgtcgc cattggcgat ctgctgcatg ccgagggcca ccgcgcgcag gccggagccg
     2821 cacagctggt tgatgccgaa cgcagtcact tccaccggaa tgccggcggc gacggcggcc
     2881 tgacgggccg ggttctggcc ctggccggct gtcaggatct ggcccatgat gacctcgtcg
     2941 acttcattcg gggcaacctt ggcgcgggaa agggctgcct tgatggcctc ggctcccagc
     3001 tggtgcgccg acatgtcggc aaaggcgcca gaaaagctgc cgaccggggt gcgggcgacg
     3061 ctggcaatga cgatttcgtt acgggccatt cgggttctcc tcaatcctgt atgtcagcgg
     3121 gcttgctgca agctcggtct tctatcgatc cagcgacaga aagcattgcg agggcttccg
     3181 tcaagaggga taagattagg taattgggcg tgcatgaagg ccatccacgc agcgcagctt
     3241 taccgtcctt gagcgaaact gctgaaggtt tgcgcaaaac ctgtatgttg ggttagactg
     3301 ccgaccctag cggagccacc ctgccgtccg cgactcgctg gagccaagga tccaaatggc
     3361 gcacgacaag accgcccatg tcatcaagaa atatgccaac cgcaggctat atcataccgg
     3421 caccagcgcc tatgtgacgc tcgacgacct cgcggacatg gtcaagtccg gacaggattt
     3481 catcgtcaac gatgccaaga ccggcgagga catcacccat tcggtgctga cgcagatcat
     3541 cttcgaacag gaaagcaagg gcgaaaacct gctgccggtc aatttcctca ggcag
//
LOCUS       DAOMXG010000210         3586 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_14294_length_3586_cov_8.999717, whole genome shotgun sequence.
ACCESSION   DAOMXG010000210 DAOMXG010000000
VERSION     DAOMXG010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14294_length_3586_cov_8.999717"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..1397)
                     /locus_tag="PLQ11_11410"
     CDS             complement(66..1397)
                     /locus_tag="PLQ11_11410"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRK25550.1"
                     /translation="MRVIAVLLLMVAGLANAQAGPERRITERVFFIPDPGASTVRFQM
                     LVLAGCGDEPGGDCRGIAHYLEHLILVGRNSQHSDQALRFFADGFSNGWTNLKATGYV
                     HSFPAGAADAPARLEQLFAFYAARLRYFAITEQDAQRERAVVRQEYDWRYGGNAHAPM
                     WKEVSRFLFPGHPMGQWAIGDPARFDLLTVESARAFHAQWYRLSNVYLFVTGPVDEAL
                     VRTLTERHLAPLPADPPPERAWLANPPALKPERIVLSHQDPRIGEPSVAFWKAVQLLR
                     PDPIRLKAVVVLLNEFLKSRITGSPHSHMVEHDEIALSIGSARIENGLHGVLQASLTV
                     VPESDVPDDRIAETAAGYFAVLAEKGLDERTLGRLKRRLAVVHERQLADPQEAASRLS
                     DWLSAGLPYERLKDFPGVVEALTPADIAIVLRALAEPGREAIVHYRPAGQP"
     gene            complement(1471..2877)
                     /locus_tag="PLQ11_11415"
     CDS             complement(1471..2877)
                     /locus_tag="PLQ11_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014331408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRK25551.1"
                     /translation="MLILIGGFLILMVLGLPVALSMALSSLVYILYSGVTPDVTLAQR
                     MIAGVESFPLLAVPFFILAGNLMNIAGVTGRIYRFAVALVGWMKGGLGQVNIIGSVIF
                     AGMSGTAIADAAGLGTIEIKAMKDHGYSTEFAVGVTAASATLGPIIPPSLPFVIYGMM
                     ANVSIGALFLAGLVPGVFMTLLMMGTVAFFAHRNGWGADTPFSWREVGGALVEVLIVL
                     AFPLAVWLMIQAGMSVNLAVGLGLALLLAADWYFDFSAVMALMAPVILIGGMTMGWFT
                     PTEAAVAAVIWSLFLGLVRYRSMTLKLVAKATFDTIETTASVLFIVTAASIFAWLLTV
                     SQAAQLLTDAILGFTSNKWVFLLLANVLILIVGCFIDTIAAISILVPILLPIVVKLGI
                     DPVHFGLIMVLNLMIGLLHPPLGMVLFVLARVAKMSVERTTMAILPWLVPLLLALIAI
                     TYIPEITLAVPRYFGMVK"
     gene            complement(2879..3451)
                     /locus_tag="PLQ11_11420"
     CDS             complement(2879..3451)
                     /locus_tag="PLQ11_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019997612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRK25552.1"
                     /translation="MANEIHSKVDADELAHTFDEQQAVDLGHIAVEDWLTLSIFWLMA
                     LCVFLQFFTRYVLNDSFAWTEEIATYCLVVIVFLGSSMCVRLSRHIQVDFLYRYMSER
                     VGFAVSTVIDILRVGFFAYAIKIVYRYIDLVAEEQMTTIQYPKGIFYWIVFGGFVLML
                     LRSVQVAWQNWSRRYSVLTRPGAFDGSEAL"
BASE COUNT          766 a         1183 c         1119 g          518 t
ORIGIN      
        1 gcccgcgccg atggccggcg ccgccagccc ggccgcaagg agcctcatcg cgtcgcgccg
       61 gtggatcatg gctggcctgc cggacgatag tggacgatcg cttcgcgtcc cggctcggcc
      121 aacgcccgca gcacgatcgc gatatccgcc ggcgtcagcg cctcgacaac gccgggaaaa
      181 tccttcaacc gctcataggg caggccggcc gaaagccaat cggagaggcg tgaggcagcc
      241 tcctgcggat cggccaattg gcgctcatgg acgaccgcca gccgtcgctt gagccggccg
      301 agggtgcgct catccagccc tttctccgcc agcacggcga aatagccggc agcagtctcg
      361 gcgatgcggt cgtccggaac gtcgctttca ggcaccaccg tcagcgaggc ctgcaacacg
      421 ccgtgcaatc cgttttcgat ccgcgcggaa ccgatcgaca aggcgatttc atcatgctcg
      481 accatgtggc tgtgcggact gccggttatc cggctcttga ggaattcgtt gagcagcacg
      541 accacggcct tgagcctgat cggatcgggg cgcagcagct gaaccgcctt ccagaaggca
      601 accgacggct cgccgatgcg cggatcctga tgcgaaagaa cgatccgctc cggcttcagc
      661 gccggagggt tggcaagcca ggcccgctcg ggtggcggat cggcgggcag gggcgccaga
      721 tgccgctcgg tgagggtccg caccagagcc tcgtccaccg ggccggtgac gaacagatag
      781 acgttggaca gccgatacca ttgcgcatgg aaggcccgcg ccgactcgac cgtcagcagg
      841 tcgaaccgcg ccggatcgcc gatggcccac tggcccatcg ggtgtccggg aaacaggaag
      901 cggctcacct ctttccacat cggcgcatgc gcattgccgc cgtagcgcca atcgtattcc
      961 tggcgcacca cagcacgttc gcgctgggcg tcctgctcgg tgatggcgaa atagcgcagc
     1021 cgtgccgcat agaaggcaaa gagctgttcg agccgggccg gtgcatcggc cgccccggcc
     1081 gggaaggaat ggacgtaacc ggtcgccttc agattggtcc agccattgga aaatccgtcg
     1141 gcgaaaaagc gcagggcctg atcggaatgc tggctgttgc gcccgaccag gatcagatgt
     1201 tcgagataat gggcgatgcc gcggcaatcg ccgcccggct cgtcaccgca gccggccagc
     1261 accagcatct ggaaacgcac ggtgctggcg ccggggtcgg gaatgaagaa cacccgttcg
     1321 gtgatccgtc gctccggccc tgcctgagca ttggccagcc cggcaaccat caacagcaga
     1381 accgcgatca ctcgcatgcc tgacctccga tccgtcatcg gatgttgcga caacgccctc
     1441 gatcgctcaa gtggcaagag gtcgcgcggc ctatttgacc atgccgaaat agcgcggcac
     1501 cgccagcgtg atctccggaa tataggtgat cgcgatcagc gccagcagca gcggcaccag
     1561 ccagggcagg atcgccatgg tggtccgctc gaccgacatc ttggcgaccc gcgccagcac
     1621 gaacagcacc atgccgagcg gcgggtgcag caggccgatc atcaggttca gcaccatgat
     1681 aaggccgaaa tgcaccggat cgatgccaag cttgacgacg atgggcagca gaatcggcac
     1741 caggatcgag atcgcggcga tcgtgtcgat gaaacagccg acgatcagga tcagcacatt
     1801 ggccagcagc aggaaaaccc atttgttcga ggtgaagccg agaatcgcat cggtgagcag
     1861 ctgggcagcc tgcgagaccg tcagaagcca ggcgaaaatc gacgccgccg tcacgatgaa
     1921 cagcaccgag gcggtcgtct cgatggtgtc gaacgtcgcc ttggccacca gcttcagcgt
     1981 catcgaccgg tagcgcacaa ggccgaggaa caggctccag attaccgcgg ccaccgccgc
     2041 ttcggtcggg gtgaaccagc ccatcgtcat gccgccgatc aggatcaccg gcgccatcag
     2101 cgccatcacc gccgagaagt cgaaatacca gtcggcggcc agcagtagcg ccagcccgag
     2161 tccgacggca agattgaccg acatgccagc ctgaatcatc agccagaccg ccagcgggaa
     2221 ggcaagcacg atcagcacct cgaccagagc accgccaacc tcgcgccagg agaacggcgt
     2281 atcggcgccc cagccgttcc tgtgggcgaa gaaggccacc gtgcccatca tcagcagtgt
     2341 catgaacacg ccgggcacca gcccggccag aaacagtgcg ccgatcgaga cattcgccat
     2401 catgccgtag atgacaaacg gcagcgaggg cggaatgatc ggccccaagg tcgccgacgc
     2461 ggccgtgact ccgacggcga actcagtcga atagccgtgg tccttcatcg ccttgatctc
     2521 gatggtccct agaccggcgg catcggcgat ggccgtgccc gacatgccgg cgaaaatcac
     2581 cgatccgatg atgttgacct gcccgagccc acccttcatc cagccgacca gcgccaccgc
     2641 aaagcgatag atccgcccgg tcacgccggc aatgttcatc agattgccgg caaggatgaa
     2701 aaacggcacc gccagcagcg ggaagctctc gacgccggcg atcatgcgct gggccagcgt
     2761 cacatcgggg gtcacgcccg aatagaggat atagacaagc gaggacagcg ccatcgacag
     2821 ggccaccggc aggccgagca ccatcaggat gaggaagcca ccgatcagga tcagcatgtc
     2881 agagcgcctc cgagccgtcg aatgcaccgg ggcgggtcag caccgaatag cggcgcgacc
     2941 agttctgcca ggcaacctgg accgagcgca gcagcatcag cacgaagccg ccgaacacga
     3001 tccagtagaa gatccccttg ggatactgga tggtcgtcat ctgctcttcc gccaccagat
     3061 cgatgtagcg gtagacgatc ttgatcgcat aggcgaaaaa cccgacccgc aggatgtcaa
     3121 tcacggtcga aacggcaaat ccgacccgct ccgacatgta gcgatagaga aaatcgacct
     3181 ggatgtgccg cgacagccgc acacacatcg acgagccgag aaacacgatc accaccaggc
     3241 aataggtggc gatttcctcg gtccaggcaa aggaatcgtt gagcacgtag cgggtgaaga
     3301 actggaggaa aacgcacagg gccatcagcc agaagatgct cagcgtcagc cagtcctcga
     3361 cggcgatatg gccaaggtcc accgcctgct gttcgtcaaa ggtgtgcgcc agttcgtcgg
     3421 cgtcgacctt gctgtggatt tcgttggcca tcgtgcccca agccctcgcc acgctggctc
     3481 ctgccgggag ccagccgatt gtcctgccat ccgctgcgac cgggaggcgg cgccggcagg
     3541 cggatgaggg ggcttgccct cacccggccc gccgccgcac gcgtcg
//
LOCUS       DAOMXG010000211         3539 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_14584_length_3539_cov_12.933697, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000211 DAOMXG010000000
VERSION     DAOMXG010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14584_length_3539_cov_12.933697"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2787)
                     /gene="uvrA"
                     /locus_tag="PLQ11_11425"
     CDS             complement(<1..2787)
                     /gene="uvrA"
                     /locus_tag="PLQ11_11425"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRK25553.1"
                     /translation="MSPLDDLFDSARPTSDSRVIAIRGAREHNLKNVDLVIPRDRLVV
                     FTGLSGSGKSSLAFDTIYAEGQRRYVESLSAYARQFLEMMQKPDVDQIDGLSPAISIE
                     QKTTSRNPRSTVGTVTEIYDYMRLLWARVGVPYSPATGLPIESQTVSQMVDRVLALPE
                     RTRGYLLAPVVRGRKGEYRKELAEFLKRGFQRVKIDGVLHELADTPSLDKNIKHDIEV
                     VVDRIAIRPDLGSRLADSVETALELADGIAIFEWADETDESGQPRRLTFSSRFACPVS
                     GFTIPEIEPRLFSFNNPFGACPTCGGIGSELKIDPDLIVPDPGLSLKKGAVAPWAKST
                     SPYYAQTLEALARHYGFSMTVPFSALPEKGRDAILFGSRGEVVALAYNDGLRAYEVKK
                     PFEGVIPNLERRFKETDSDWAREEIGRFMAETPCASCAGYRLKPEALAVKIAGRHIGE
                     VARLSVRDAATWYAALPEALTPKQAEIAGRILKEIRERLRFLNDVGLEYLTLARGSGS
                     LSGGESQRIRLASQIGSGLTGVLYVLDEPSIGLHQRDNDRLLGTLKRLRDLGNSVIVV
                     EHDEDAIRMADYVVDVGPGAGIHGGEIVARGTPQEILDDPNSLTGQYLSGRLTVPMPK
                     RRRTPQKARQLRIVNARGNNLKSVTCDVPLGLFTCITGVSGGGKSTLVIETLYRAAAR
                     KLSGALEHPAPHDRIEGLEMIDKVIDIDQSPIGRTPRSNPATYTGAFTPIREWFAGLP
                     EAKARGYQPGRFSFNVKGGRCEACQGDGVIKIEMHFLPDVYVTCDVCKGKRYDRETLE
                     VKFRGKSIADVLDMSVEEAAELFRAVPSIRDKMETLARVGLNYISVGQQATTLSGGEA
                     QRVKLSKELAKRATGRTLYILDEPTTGLHFHDVAKLMEVLHELVDQGNTVVVIEHNLD
                     VIKTADWVIDLGPEG"
     gene            complement(3038..>3539)
                     /locus_tag="PLQ11_11430"
     CDS             complement(3038..>3539)
                     /locus_tag="PLQ11_11430"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003602369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRK25554.1"
                     /translation="LDLPGYALGGLAVGEPQAEMLATIEATEPFLPADKPRYLMGVGT
                     PDDILEAIRRGIDMFDCVMPTRAGRHGTAYTRFGKMNLKNARHAEDRRPLDVTSSCPA
                     SRDFSRAYLHHLIKSGEMLGDMLLTWNNVAYYCDLVDGARAAILAGRYADYVAAVKED
                     WERGDE"
BASE COUNT          632 a         1124 c         1139 g          644 t
ORIGIN      
        1 gccttcggga ccaagatcga tcacccagtc ggcggtcttg atcacgtcga gattgtgctc
       61 gatcaccacc accgtattgc cctgatcgac cagttcatgc agcacctcca tcagcttggc
      121 gacatcgtgg aagtgcaggc cggtggtcgg ctcatcgaga atgtagagcg tgcgcccggt
      181 ggcgcgcttg gccagttcct tggagagttt gacccgctgc gcctcgccgc cggacagagt
      241 ggtcgcctgc tggccgacgg agatgtagtt gagcccgacc cgcgccagcg tttccatctt
      301 gtcgcggatc gacggcaccg ccctgaacag ctcggccgcc tcctcgacgc tcatgtcgag
      361 cacatcggcg atcgacttgc cgcggaactt cacctccagc gtttcgcgat cgtagcgctt
      421 gcccttgcag acatcgcagg tgacatagac atccggcagg aagtgcatct cgatcttgat
      481 cacgccatcg ccctggcatg cttcgcagcg accgcccttg acgttgaagg aaaagcgccc
      541 cggctggtag ccgcgcgcct tggcctccgg caggccggcg aaccattcgc ggatcggcgt
      601 gaaggctccg gtataggtcg ccgggttgga gcggggggtg cggccgatcg gcgactggtc
      661 gatgtcgatc accttgtcga tcatctccag cccctcgatc cggtcatgcg gggcagggtg
      721 ctccaacgct ccggacagct tgcgcgccgc cgcccgatac agcgtttcga tcaccagcgt
      781 cgacttgccg ccgccggaga cgccggtgat gcaggtgaac aggccgagcg gcacatcgca
      841 ggtcaccgat ttgagattgt tgccgcgcgc attgactatc ctgagctgcc gcgccttctg
      901 cggcgtgcgc cggcgcttcg gcatcggcac ggtgaggcga ccggagagat attgcccggt
      961 cagcgagttc ggatcgtcga gtatttcctg cggcgtgcca cgggcaacga tctcgccgcc
     1021 atggatgccg gcgccggggc cgacatcgac cacataatcc gccatccgga tggcgtcctc
     1081 gtcgtgctcg acgacaatca ccgaattgcc gagatcgcgc aggcgcttca gcgtgcccag
     1141 caggcggtcg ttgtcgcgct ggtgcaggcc gatcgacggc tcgtccagca catagagcac
     1201 gccggtcagt cccgaaccga tctgtgacgc cagccggatg cgctggcttt ctccgccgga
     1261 caacgagccg gagccgcgcg ccagcgtcag atattcgagg ccgacatcat tgagaaagcg
     1321 caggcgttcg cggatttcct tgagaatgcg tccggcgatc tccgcctgct tgggggtgag
     1381 ggcttcgggc agcgcggcat accaggtcgc cgcatcgcgc accgacagcc gtgccacctc
     1441 gccgatgtgc cggccggcga tcttgaccgc cagcgcctcg ggcttcaacc ggtagccggc
     1501 acaactggcg caaggcgtct cagccatgaa ccggccgatt tcctcgcggg cccagtcgct
     1561 gtcggtttcc ttgaagcgcc gttcgaggtt ggggatgacg ccttcgaacg gcttcttgac
     1621 ctcgtaggcg cgcaatccgt cattataggc tagggcgacg acctcgccgc gcgatccgaa
     1681 caggatcgca tcgcgcccct tttccggcag cgcgctgaac ggcacggtca tcgaaaaccc
     1741 gtaatgccgg gcgagcgcct ccagcgtctg ggcataatag gggctggtgg atttggccca
     1801 gggggcgaca gcgcccttct tgaggcttag gccggggtcc ggaacgatca gatcggggtc
     1861 gatcttcagt tccgaaccga tgccgccgca ggtcgggcag gcgccgaacg gattgttgaa
     1921 cgagaacagc cgcggctcga tctccggaat ggtgaagccg gagaccgggc aggcaaagcg
     1981 cgaggaaaac gtcagccgcc ggggctggcc ggattcatcc gtctcgtccg cccattcgaa
     2041 tatggcgatg ccatcggcca gttccagcgc ggtttcaacg ctgtccgcca ggcgcgagcc
     2101 aaggtccgga cggatggcaa tccgatccac cacaacctcg atgtcgtgct tgatgttctt
     2161 gtccaggctc ggcgtatcgg ccagttcgtg caggacaccg tcgatcttga cgcgctgaaa
     2221 gccgcgttta agaaattccg ccagttcctt gcggtattcg cccttgcggc cgcgcaccac
     2281 cggagccagc aggtagccgc gtgtgcgctc gggcagtgcc agaacccggt ccaccatctg
     2341 gctgaccgtc tggctctcga tcggaagccc ggttgcgggc gaatagggca caccgacccg
     2401 cgcccacaac agccgcatgt aatcgtagat ctcggtgacg gttccgaccg tcgagcgcgg
     2461 attgcgggag gtcgtcttct gttcgatcga aatggccggc gacaggccgt cgatctggtc
     2521 aacgtccggc ttctgcatca tttcgaggaa ctggcgggca taggccgaca ggctctcgac
     2581 atagcggcgc tggccctcgg catagatcgt atcgaaagcc agcgacgact tgcccgatcc
     2641 cgacaggccg gtgaacacca ccagccgatc gcgggggatg acaagatcga cgttcttgag
     2701 attgtgctcg cgtgcaccgc ggatcgcgat gacgcggcta tcggaggtcg ggcgggcgga
     2761 atcgaaaaga tcgtcgagcg gagacatcag caaccagaca tcaggcgagg ggcaatccga
     2821 agtccaggca aagcccggcc gtcagggggt gtcgtcaaga taggcttggt caggcgggcg
     2881 caacaacccc tagagcatgt cgcgattagg tggaatccgg ctaatcgcag aagacatgct
     2941 ctaacttatt gataccgatc acgttatccg cattttgatg attccatcaa aatgcggcgt
     3001 gatcttgtcc aacaagttat ggcgctggcc tgcggcctca ttcgtctccg cgctcccaat
     3061 cctctttcac cgcagcgaca taatcggcat agcgccccgc caggatggcg gcgcgggcgc
     3121 catcgaccag atcgcagtaa taggcgacat tgttccatgt cagcagcatg tcgcccagca
     3181 tctcgcccga tttgatcaga tggtgcaggt aggcgcgcga aaaatcgcgg ctcgccggac
     3241 aggaggacgt cacgtcgagc ggccggcgat cctcggcgtg gcgggcgttc ttgaggttca
     3301 tcttgccgaa gcgggtgtag gcggtgccat ggcgtccggc gcgggtgggc atgacgcaat
     3361 cgaacatgtc gatgccgcgg cggatcgctt cgagaatatc gtctggcgtg ccgacgccca
     3421 tcaggtagcg cggcttgtcg gcgggcagaa acggttcggt cgcctcgatg gtcgccagca
     3481 tctcggcctg cggctcgccc accgccagcc cgcccagcgc gtagccgggc agatcgagc
//
LOCUS       DAOMXG010000212         3536 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_14614_length_3536_cov_9.877047, whole genome shotgun sequence.
ACCESSION   DAOMXG010000212 DAOMXG010000000
VERSION     DAOMXG010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3536
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14614_length_3536_cov_9.877047"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..1871
                     /locus_tag="PLQ11_11435"
     CDS             141..1871
                     /locus_tag="PLQ11_11435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HRK25555.1"
                     /translation="MAKNTSGQAATIAFFPCHAALLASAAVVLGMGTAHGAEIEARSR
                     IQAVTVFPDAAIVTRSVPVELEAGSHVLVLRGLPASLDPASLRIEGKATGALSLGAVD
                     MRLVPIDPAKAGGESEARLKAAREERDRLAAILDGHEGRKAMIQRFAQSGPERGESAE
                     GLKIDQWASAWATVGEALVKVNEDVRLARRAVDEAEAKLKAAEQAETGRRNRTAPERD
                     FTVALEAGEAVKAELTLSYRVSGAGWRPGYDARLATGVGGRGATLSLSRRAVVTQRTG
                     EDWSDVEMALSTVRAFRGTAAPDVIPQRVGFYEPPMPVPMARQGAGRMAEAETEDGTR
                     LKAAAPPPMMAARPMNEVVAPKVAEVESGDFQTSYRLPGKVSLAQDGTQKSFIIHSRE
                     IAPDLLVRSAPALDPSAYLEASFINQEDAPLLAGEVNLIRDGSYVGKGRFGFVAPGEK
                     AILGFGADDRVKVMRVPVRRKETDPMLVGTNKTDTREFKITVRNLHDMPIKAEIVDQM
                     PYSEMANLTVEPLNGMTQPTEKSPGDRRGVMVWAYEIKPKEEKEIRLGYRLRWPSDRE
                     ISFTRVPVPK"
     gene            2005..3300
                     /locus_tag="PLQ11_11440"
     CDS             2005..3300
                     /locus_tag="PLQ11_11440"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HRK25556.1"
                     /translation="MANVVVVGAQWGDEGKGKIVDWLSEQADVVVRFQGGHNAGHTLV
                     IDGKVYKLSLLPSGIVRSGKLSVIGNGVVVDPFALVEEIERLKGQGVAVTPASLRLAD
                     NAALILPLHQELDGYRESGASAGTKIGTTKRGIGPAYEDKVGRRAIRVMDLVEPAALD
                     GKIARLLAHHNALRRGLGLPEVDGAALKARLVEVAPKILPYMDRVWDLLDGKRKAGKR
                     ILFEGAQGALLDIDHGTYPYVTSSNTVAGQAAAGSGLGPGAVGYVLGIVKAYTTRVGE
                     GPFPTELTDETGRLIGERGREFGTVTGRARRCGWFDAALVRQTVCNNGIDGIALTKLD
                     ILDGFKTIRICTGYRLDGKVLDFLPAGEAAQARVEPIYETIEGWAGATAGARSWADLP
                     AQAIKYVRRIEELIGAPVALLSTSPERGDTILVRNPFEG"
     gene            3310..>3536
                     /locus_tag="PLQ11_11445"
     CDS             3310..>3536
                     /locus_tag="PLQ11_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25557.1"
                     /translation="MADYLPLIQRAVAALDPNTRERREAIYARAREALNRQLLSLDPP
                     IATVDLQRERNALDDTIRIVETAYAPAQVAPP"
BASE COUNT          625 a         1062 c         1268 g          581 t
ORIGIN      
        1 agagcatgtc ttctgcgatt gaccggtatc cacttaatcg cgacatgctc tagccggaaa
       61 aacgccttgg gatttcccga ttcggcgcca tgatcatcct ccggtgatcg gaccggcgat
      121 gcgcgccgca ggaggatttc atggcaaaga atacatccgg ccaggcggca accatcgcat
      181 ttttcccttg tcatgcggcc ttgctcgcct cagcggctgt ggtgctgggc atggggacgg
      241 cgcatggcgc cgagatcgag gcaaggtcgc gcatccaggc ggtgacggtg tttcccgatg
      301 ccgcaatcgt gacccgatcg gttccggtgg agttggaggc aggcagccat gtgctggtgc
      361 tgcgtggtct gccggccagt cttgatcctg cttcgctgcg gatcgaaggc aaggcgacgg
      421 gggcgctcag cctcggggcg gtcgacatgc ggttggtccc gatcgatccg gcaaaggcag
      481 gcggggagag cgaggcaagg ctgaaggcgg cgcgcgagga gcgcgaccgg ctggccgcca
      541 tccttgatgg ccatgaggga cgcaaggcga tgatccagcg ctttgcccag tccgggcccg
      601 aacgcgggga atcagccgag gggctgaaga tcgaccaatg ggcgtccgcc tgggcgaccg
      661 tcggggaagc gctggtcaag gtcaacgagg atgtgcgcct ggcgcggcgg gcggttgacg
      721 aggccgaggc aaagctcaag gcggcggaac aggccgagac cgggcggcgc aaccggacag
      781 cgccggaacg tgatttcacc gtggcgctgg aagccggcga agccgtcaag gcggaactga
      841 cgctgtccta tcgggtcagc ggtgccggct ggcggccggg ctatgacgcc cggcttgcaa
      901 ccggtgtggg cgggcgcggc gcgacgttgt cgctcagccg ccgggcggtg gtgacccagc
      961 gcacgggcga ggactggagc gatgtcgaga tggcgctctc gacggtgcgg gcgttccgtg
     1021 gcacggcggc gccggacgtg attccccagc gcgtgggctt ttacgagccg ccgatgccgg
     1081 tgccgatggc gcggcagggc gcgggacgga tggccgaagc cgagaccgag gacggcacac
     1141 ggctgaaggc agcagcgccg ccaccgatga tggcagcccg gccgatgaac gaggtggttg
     1201 cgccaaaggt cgccgaggtc gagagcggcg atttccagac cagctaccgc ctgcccggca
     1261 aggtcagcct ggcccaggac ggcacgcaga aatccttcat catccacagc cgcgagatcg
     1321 ctcccgatct cctggtgcgt tcggccccgg cgcttgatcc gtccgcctat ctggaagcct
     1381 cgttcatcaa tcaggaggat gcgccgctgc ttgccggcga ggtcaacctc attcgcgatg
     1441 gcagctatgt cggcaagggg cggttcggtt tcgtcgcgcc gggggagaag gccattctcg
     1501 gtttcggggc agatgatcgt gtcaaggtga tgcgcgtgcc ggtcaggcgc aaggaaaccg
     1561 atccgatgct ggtcggcacc aacaagaccg atacgcgcga gttcaagatc accgtcagga
     1621 atctgcatga catgccgatc aaggccgaga tcgtcgatca gatgccctat tcggaaatgg
     1681 ccaacctgac ggtcgagccg ctgaacggga tgacgcaacc gaccgaaaag agccccggcg
     1741 accgccgtgg tgtgatggtc tgggcctatg agatcaagcc gaaggaggag aaggaaatcc
     1801 gtctcggcta tcggttgcgc tggccatcgg atcgcgagat cagcttcacc cgggtgccgg
     1861 tgccgaaata gccgcaccac ctccgcgctt tacgctgccc ggcccggcgg ctatagcgac
     1921 aggacgtcaa ccggacgacg cgccgtcgtc gtgccggtcg tttgtgtctc aactggcaga
     1981 gagccaagag gacgaatacg cgctatggcc aatgttgtgg tggtcggcgc ccagtggggc
     2041 gacgagggca agggaaagat cgtcgactgg ctctccgagc aggccgatgt ggtcgtgcgc
     2101 ttccagggcg ggcacaatgc cggccatacg ctggtcattg acggcaaggt ctacaagctg
     2161 tcgctgcttc cctccggcat cgtgcgctcc ggcaaattgt cggtgatcgg caatggcgtg
     2221 gtggtcgatc cgtttgcgct ggtcgaggag atcgagcggc tgaaggggca gggcgttgcg
     2281 gttacgcccg cgtcgctgcg gctggcggat aacgcggctc tgattctgcc gctgcatcag
     2341 gaactcgatg gctatcgcga atcgggggcg agcgcgggca ccaagatcgg caccaccaag
     2401 cgcgggatcg ggcccgccta tgaggacaag gtcggccgcc gtgccatccg tgtcatggat
     2461 ctggtcgagc cggcggcgct ggatggcaag atcgctcggc tgctcgccca tcacaatgcg
     2521 ctgcggcgcg gactcggcct gccggaggta gatggcgcgg cgctgaaggc caggctcgtc
     2581 gaggtggcgc cgaaaatcct gccctatatg gatcgggtgt gggacctgct cgatggcaag
     2641 cgcaaggcgg gcaaacgcat cctgttcgag ggggcgcagg gcgcgctgct cgatatcgat
     2701 cacggcacct atccctatgt cacctcgtcc aacacggtgg ccgggcaggc ggcggcgggt
     2761 tcgggcctcg ggccgggggc ggtcggctat gtgctcggca tcgtcaaggc ctataccacg
     2821 cgcgtcggcg aggggccgtt cccgaccgaa ctcaccgacg agaccggacg gctgatcggc
     2881 gagcgcgggc gcgaattcgg cactgtcacc ggccgggcaa ggcgttgtgg ctggttcgat
     2941 gcggcgctgg tgcgccagac cgtgtgcaac aacggcatcg acggtatcgc actgaccaag
     3001 ctcgacattc tcgatggatt caagaccatc aggatttgca cgggctatcg gctcgatggc
     3061 aaggtgctcg atttcctgcc ggccggcgaa gcggcgcagg cgcgggtcga gccgatctac
     3121 gagacgatcg aaggctgggc tggggcaacc gccggggcgc gttcctgggc tgatcttccg
     3181 gcccaggcca tcaaatacgt ccggcgcatc gaggagttga tcggggcgcc ggtggcactg
     3241 ttgtcgacgt cgccggaacg tggcgatacg attctggtgc gcaacccgtt cgaaggctga
     3301 tcgcaaggca tggccgatta tctcccgctc atccagcgtg cagtcgcagc gctcgatccc
     3361 aatacgcggg agcggcgtga ggcgatctat gcgcgggccc gggaagcact gaaccgacag
     3421 ctgctgagcc ttgatccacc gatcgcgacg gtcgatcttc agcgcgaacg caatgcgctc
     3481 gacgatacga tccgcatcgt cgagacagcc tacgcgccgg cacaggtcgc accgcc
//
LOCUS       DAOMXG010000213         3529 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_14654_length_3529_cov_13.432642, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000213 DAOMXG010000000
VERSION     DAOMXG010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14654_length_3529_cov_13.432642"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..842
                     /locus_tag="PLQ11_11450"
     CDS             183..842
                     /locus_tag="PLQ11_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HRK25558.1"
                     /translation="MCGRYALTLPPAAVRAYFAYREQPNFPPRYNIAPTQPIALVRRQ
                     DGARQFALARWGFLPGFVRDMREFPLILNARAEGIADKPSFRAAIRRRRCLIPADGYY
                     EWQRAGRFKRPFLIRRPNRGPIAFAGLWETFHSPDGSEMDTACIITTAANATIAAVHE
                     RMPVVIEPGDFDLWLDPGSSGGDVLALLRPAPDDALELLPVSDAVNRATTEGPEVQEP
                     A"
     gene            complement(922..1521)
                     /locus_tag="PLQ11_11455"
     CDS             complement(922..1521)
                     /locus_tag="PLQ11_11455"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018264217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRK25559.1"
                     /translation="MNGLRMIAAAVVALSLAGCKYETFADPTLKGRDLELMALAPPMP
                     SDLDPIRARYRIHNPTGEKTPGTIVVDSDAKFLYLIEENGMALRYPIAVGQDAYVWSG
                     TAIIGRKAEWPNWTPGNDARALNPGLPGTVPGGPQNPLGARAIYLYDNGKDTMYRIHG
                     TNEPESIGRNQSLGCIRMYNIDVIDLYNRVKDGAKVVVL"
     gene            1909..2793
                     /locus_tag="PLQ11_11460"
     CDS             1909..2793
                     /locus_tag="PLQ11_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015951393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HRK25560.1"
                     /translation="MPDGHPERPARLQAIRQVLAQDQFASLRREGAVRAAPEAAELAH
                     PAAHVARIASAVPQGAFHFFDADTPMSPESLEVALHAVGSAQRAVDAVMTGEVSNAFV
                     AARPPGHHAERDTAMGFCFFNNIAIAARHAQRAHRAERVAIVDWDVHHGNGTQDIFWS
                     DRSVLFCSTHEMPLYPGTGAGRETGEHNTIVNVPLAAGDDGRVFKEHLRDAILPRIRA
                     FAPDLILISAGFDAHHRDPLANIRLTEQDFAWATEAMMEVAAIKCNGRLVSLLEGGYD
                     LQGLAFSVGAHVNTLMGA"
     gene            complement(2912..>3529)
                     /gene="aroC"
                     /locus_tag="PLQ11_11465"
     CDS             complement(2912..>3529)
                     /gene="aroC"
                     /locus_tag="PLQ11_11465"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HRK25561.1"
                     /translation="GALVQIGPHRIDRSRWDWEEVDRNPFFSPDPQSVEMFSDYLDGI
                     RKSGSSIGAVIEVVAEGVPAGLGAPVYGKLDSDLASALMSINAVKGVEIGAGFGAAEL
                     TGEDNADEIRIGNDGQVRFMANHAGGILGGISTGQPIIARFAVKPTSSILSPRKSIDR
                     FGNEIDILTKGRHDPCVGIRAVPVGEAMMALVLADHYLRHRAQVG"
BASE COUNT          625 a         1142 c         1085 g          677 t
ORIGIN      
        1 cccgcgttgc gcaggcagca gggtcggaca atcccgcgct tgcccgccgc atcatggatg
       61 cgcgcttctt tatcgagaat cacgcctcga ccgccggcgg actggaaatt tccattctgg
      121 aaggagcgga cgagatcaac gccgcctatg cggaaggcgc ctgaaaccgg gcggcggcgg
      181 gcatgtgcgg ccgttacgcg ctgaccttgc cgcccgcagc ggtgcgggcc tatttcgcct
      241 accgtgagca gccgaatttc ccgccgcgct acaacatcgc gcccacccag ccgatcgcgc
      301 tggtgcggcg gcaggacggg gcgcggcagt tcgctttggc ccgctgggga tttctgccgg
      361 gctttgtcag ggacatgcgc gaatttccgc tcatcctcaa tgcgcgcgcc gagggcatcg
      421 ccgacaagcc gtcgttccgt gccgccatcc gccggcgacg ctgcctgatc ccggccgatg
      481 gctattacga atggcaacgc gccggccgct tcaaacgccc gttcctgatc cgccggccga
      541 accgtggccc gatcgctttt gccggactgt gggagacctt ccattccccc gatggcagtg
      601 agatggacac ggcctgcatc atcaccaccg ccgccaatgc gaccattgcc gccgtgcatg
      661 agcgcatgcc ggtggtgatc gagcccggcg atttcgacct ctggctcgat cccggcagca
      721 gcggtggcga tgtgctggcg ctgctccggc ccgcgccgga tgatgcgctg gagctgttgc
      781 cggtaagcga tgccgtcaac cgggcgacaa ccgaagggcc tgaggtgcag gaacccgcct
      841 gaatggtgcg caaaatgcaa gcattgcacg aaaaagggcg gccgaagccg cccctgcatg
      901 ccttgaagtc cagacgatgg cttacagcac cacaaccttt gcgccgtcct tgacgcggtt
      961 gtagaggtcg atcacgtcga tgttgtacat ccggatgcag cccagcgact ggttgcgacc
     1021 gatcgactcc ggctcgttgg tgccatggat gcgatacatc gtatccttgc cgttgtcgta
     1081 gagatagatc gcccgggcgc cgagcgggtt ctgcgggccg ccgggaacgg tgcccggcag
     1141 gccggggttg agcgcgcggg catcgttgcc cggtgtccag ttcggccatt ccgccttgcg
     1201 gccaatgatc gcggtgccgg accacacata ggcatcctgc ccgacggcga tcggatagcg
     1261 cagcgccatc ccgttctcct cgatcaggta gaggaacttt gcatccgaat cgacgacgat
     1321 ggtgcccggg gtcttttcgc ctgtcgggtt gtggatgcgg tagcgggcgc ggatcggatc
     1381 gagatcggac ggcatcggcg gggcgagcgc catcagttcg agatcgcgcc ccttgagggt
     1441 cggatccgca aacgtttcat acttgcaccc cgccagcgac agcgccacca ccgccgccgc
     1501 aatcatccta agcccgttca ttgcccaacc tgccgtatcg tgaggtcatt cacgatctgt
     1561 gaaccatctt tcttcacgaa gcgtaaacca cgcatcgttt tctgcgcctg tgcgcaaaca
     1621 cacccatgaa cgtctgatta accatttcat cgtccgtgtc catgccgaat gtcgcgggta
     1681 aggccaaaat gtctgattgt ctccccggct gtggcatgca tgctagagca tgctgcgatc
     1741 aggtggaatc cggctgatcg caacaagcat gctctaagcc attgataacg atcacgttgt
     1801 ctaacgtttt gaggtttcaa acaaaacggg agcgtgatcc ggagtgccat tgaggagaga
     1861 ccggtgccga ccctgctgat cgaggatgag ctgtttcagc gccatgtgat gcccgacggc
     1921 catccggaac gtcccgcccg gttgcaggcg atccgccagg tgctggcgca ggatcaattt
     1981 gcctcactgc ggcgggaagg ggcggtgcgg gctgcgcctg aggcagcgga gcttgcccat
     2041 ccggcggcgc atgttgcacg catcgccagc gcggttccgc aaggcgcctt ccatttcttc
     2101 gatgccgata cgccgatgtc ccctgagagc ctggaggttg ccctgcacgc ggtcggctcc
     2161 gcccaacgcg cggtcgatgc ggtgatgacc ggcgaggtgt cgaatgcctt cgtcgccgcc
     2221 cgcccgcctg gtcatcatgc cgagcgcgac acggcgatgg ggttctgttt cttcaacaac
     2281 attgccattg ccgcccggca tgcccagcgc gcccatcgcg ccgagcgcgt tgccatcgtc
     2341 gattgggatg tgcaccacgg caacggcaca caggacatct tctggtctga cagatcggtg
     2401 ctgttctgct ccacccatga gatgccgctt tatcccggca cgggcgccgg ccgcgagacc
     2461 ggcgagcaca acaccatcgt caatgtgccg ctcgctgccg gcgacgatgg ccgcgttttc
     2521 aaggagcatc tgcgcgatgc gatcctgccg cgcatacgcg cctttgcgcc ggatctgatc
     2581 ctgatctctg cggggttcga tgcccatcat cgcgatccac tggccaatat ccgcctgacc
     2641 gagcaggatt tcgcctgggc caccgaggcg atgatggaag tcgccgccat caagtgcaac
     2701 ggccggctcg tgtcgctgct tgaaggcggc tacgatctgc aggggctggc cttttccgtc
     2761 ggcgcccatg tcaacacgct gatgggggcg tgatcgctta gatcacgccg cattttgatg
     2821 gaatcatcaa aatgcggata acgtgatcgg tatcaataag ttagagcatg tcttctgcga
     2881 ttagccggat tccacctaat cgcgacatgc tctagcccac ctgcgcgcgg tgcctgaggt
     2941 agtgatcggc cagcaccagc gccatcatcg cttcgcccac cggcacggcg cggatgccga
     3001 cgcaggggtc gtgccggccc ttggtgagga tgtcgatttc attgccgaac cggtcgatcg
     3061 actttctcgg gctcagaatc gacgaggtcg gcttcaccgc aaagcgtgcg atgatcggct
     3121 ggccggtgga aataccgccg agtatgcctc cggcgtgatt ggccatgaaa cgaacctggc
     3181 cgtcattgcc gatccggatt tcgtcggcat tgtcctcgcc ggtcaattcg gctgcgccga
     3241 atcctgcccc gatctccacg cccttgactg cattgatgct catcagcgcg ctggcaagat
     3301 cagaatcgag cttgccatag accggcgcgc cgagcccggc cggaacaccc tccgccacca
     3361 cctcgatcac cgctccgatc gaggaaccgg acttacggat gccgtcaagg taatcggaaa
     3421 acatctccac cgattgcgga tcgggcgaaa agaacgggtt gcgatccacc tcctcccagt
     3481 cccagcgcga ccggtcgatg cggtgcgggc cgatctgaac cagcgcgcc
//
LOCUS       DAOMXG010000214         3470 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_15023_length_3470_cov_8.700732, whole genome shotgun sequence.
ACCESSION   DAOMXG010000214 DAOMXG010000000
VERSION     DAOMXG010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15023_length_3470_cov_8.700732"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..122)
                     /locus_tag="PLQ11_11470"
     CDS             complement(<1..122)
                     /locus_tag="PLQ11_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018063064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase TrpC"
                     /protein_id="HRK25562.1"
                     /translation="MTDILARIETTKRAEIAAAKAAVPLDEIKIDQSFVRDVLT"
     gene            276..1844
                     /locus_tag="PLQ11_11475"
     CDS             276..1844
                     /locus_tag="PLQ11_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009494101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRK25563.1"
                     /translation="MQSAPFDVAQTRVSLPEALLNAKARFGSAKEILEDPERTPLTYA
                     RLVLGSLILGRKLAALTARGARVGVLLPSVNGVAVTLFGLLFHERLPVLLNFTAGAKN
                     LLSACETAEIDVIVTSRRFIDTAKLDDVVATLGMSRTIVYLEDVRKAITGIDKVVGMA
                     RSLVAQAIARRACPDPDAPAVILFTSGSEGVPKGVVLSHANLVGNAMQIWQHAHGMLS
                     QDDIAMNPLPVFHSFGLTAGTLVGVLNGMKVVLYPSPLHYKQVPKLVHETKATLLFAT
                     DTFLVGYGRAAEAGELASLRIVVTGAEKVKETTRDLWAPYGTTILEGYGCTECAPVLS
                     CNTPDRNRPGTVGPMLPGIESRLDPVEGIHEGGKLVVRGCNVMLGYMLASRPGVIQPP
                     ADGWYDTGDIVDLDRDGFITIKGRAKRFAKLGGEMVSLAAIETIASDLWPGATHVGVT
                     LPDKRKGEQVVLVTDKPDADRDDFLAYARANGVPELYVPRAILVVNAIPVLGSGKIDY
                     AGTAELVKTMRALI"
     gene            1871..2650
                     /locus_tag="PLQ11_11480"
     CDS             1871..2650
                     /locus_tag="PLQ11_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRK25564.1"
                     /translation="MDFGLKGRTAIVCAASRGLGRGCAEALAGEGVNLVIAARTRADI
                     EATAADIAARYKVPVTAIACDVTTGEGREALLAACPNPDILITNAAGPPPGDFRNFTL
                     DDWRKAAESNFFAPIALIQATVYGMVDRRFGRIVNITSAAVKAPLAALELSNGVRAGL
                     TGAVAVLARNTIKHNVTINNLLPGLHETDRLHSNIAAAMKRTGKDLAGATADFVARVP
                     AGRFGTIEEFGAACAFLCSRQAGFITGQNLVADGGAYPGTL"
     gene            2821..2907
                     /locus_tag="PLQ11_11485"
     tRNA            2821..2907
                     /locus_tag="PLQ11_11485"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2855..2857,aa:Leu,seq:taa)
     gene            2927..3139
                     /locus_tag="PLQ11_11490"
     CDS             2927..3139
                     /locus_tag="PLQ11_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HRK25565.1"
                     /translation="MAVRDANGTELKDGDSVTLIKDLKVKGTSVTLKRGTLIKQIRLT
                     ADDEEIEGRSDKVKGLVLRTEFVKRA"
     gene            3238..>3470
                     /locus_tag="PLQ11_11495"
     CDS             3238..>3470
                     /locus_tag="PLQ11_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015823316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HRK25566.1"
                     /translation="MDSLTDYDFDLPDRLIALYPARPRDSARMLVVRPGQALEHLGVR
                     DLPALLRPGDALVFNDTRVIPARLRGVRLRGGGS"
BASE COUNT          617 a         1084 c         1123 g          646 t
ORIGIN      
        1 tcggtcagca cgtcgcgtac aaacgactgg tcgatcttga tctcgtccag cggcacggcg
       61 gctttcgcgg cggcgatttc ggcgcgtttg gtggtttcga tccgggcgag aatgtcagtc
      121 atggcggtgt tgaacccggt ttgcgcgcat ttgtcgagcg gtttgcgcgg tattgccgct
      181 tgccagggac atcggccggg ttgaccgctg caccgccgcg ccccttaatg ccgccggcac
      241 cggcgcgcaa gatcgccgga gcgcgggaga acgccatgca gagtgccccc ttcgatgtcg
      301 cgcaaacccg cgtgagcctg ccagaagcgc tgctcaacgc caaggcgcgg tttggcagcg
      361 ccaaggagat cctggaagat cccgaacgga cgccgctcac ctacgcccgc ctggtccttg
      421 gctcgctcat tcttggccgc aagctggccg ccctgaccgc ccggggcgcc cgcgtcgggg
      481 tgctgctgcc ttcggtcaac ggcgtggcgg tgacgctgtt cggcctgctg tttcacgagc
      541 ggctgccggt gctgctgaat ttcacggcgg gtgccaagaa cctgctgtca gcctgcgaga
      601 cggccgaaat cgacgtgata gtcacctcgc gccgtttcat tgacaccgcc aagctcgacg
      661 atgtggtggc aacgctcggc atgagccgca ccatcgtcta cctggaggat gtccgcaaag
      721 ccatcaccgg gatcgacaag gtggttggga tggcgcgctc actggtggca caggccatcg
      781 cccggcgggc ctgccccgat ccggacgcgc cggcggtgat cctgttcacc tccggctcgg
      841 aaggtgtgcc caagggtgtg gtgctctccc atgccaatct ggtcggcaat gccatgcaga
      901 tctggcagca tgcgcatggc atgctcagcc aggacgacat cgccatgaac ccgctgccgg
      961 tgtttcattc cttcggcttg acggcgggca cgctggtcgg cgtgctgaac gggatgaagg
     1021 tggtgctcta tccttcgccg ctgcattaca agcaggttcc caagctggtg catgagacga
     1081 aggccacgct gctttttgcc accgatacgt tcctggtcgg ttatggccgg gcggccgagg
     1141 cgggcgaact ggcatcgctg cggatcgtcg tgaccggtgc cgaaaaggtc aaggagacga
     1201 cccgcgatct ctgggcgccc tatgggacga ccattctcga aggctatggc tgcaccgaat
     1261 gcgcgccggt cctctcctgc aacacaccgg accggaatcg ccccggcacc gtcggcccca
     1321 tgctgccggg cattgaatcg cggctcgatc cggtcgaggg catccatgag ggcggcaagc
     1381 tggtggtgcg cggctgcaat gtcatgcttg gatacatgct ggcctcgcgt ccgggggtga
     1441 tccagccgcc ggctgatggc tggtatgaca ccggggatat cgtcgatctc gatcgggacg
     1501 ggttcatcac catcaagggc cgcgccaagc gctttgccaa gctgggcggc gagatggtgt
     1561 cgctggccgc gatcgagacc atcgcctccg atctctggcc gggggcgacg catgtcgggg
     1621 tcaccctgcc agacaagcgc aagggcgaac aggtggttct ggtcaccgat aagccggacg
     1681 ccgaccgtga cgacttcctt gcctatgcca gggccaacgg cgtgccggaa ctttatgttc
     1741 ctcgcgccat tctggtggtc aatgcaattc ctgttctcgg ttcgggcaag atcgactatg
     1801 cgggaacggc tgaacttgta aaaaccatgc gcgcgctcat ctgagcgacg gcaatcaaca
     1861 ggatttttcc atggattttg gtttgaaggg caggacagcc atcgtctgtg ccgcatccag
     1921 ggggctgggg cgcggctgcg ccgaggcgct ggccggcgaa ggcgtcaatc tggtcatcgc
     1981 ggcgcgaacc agggccgaca tcgaagcgac agcggccgac atcgcggcgc gctacaaggt
     2041 tccggtcaca gcgatcgcct gcgatgtgac aaccggggag gggcgcgagg cgctgctcgc
     2101 tgcctgtcca aacccggata ttctgatcac caatgccgcc ggaccgccgc ccggcgattt
     2161 ccgcaacttc acgctggatg attggcgcaa ggcggccgaa agcaatttct tcgctcccat
     2221 cgccctcatt caggccaccg tctacggcat ggttgaccgc aggttcgggc gcatcgtcaa
     2281 catcacctcc gcggcggtca aggctccgct ggccgcgctg gaattgtcga acggtgtgag
     2341 ggcaggattg accggggccg tcgccgtgct ggcgcgcaac accatcaagc acaacgtcac
     2401 catcaacaac ctgcttcccg ggctgcatga gaccgatcgc ctgcatagta acattgccgc
     2461 agcgatgaag cggaccggaa aggatctggc cggcgcaact gccgatttcg tggcgcgcgt
     2521 cccggccggc aggttcggca ccatcgagga attcggtgcg gcctgcgcct ttctctgctc
     2581 gcggcaggcg ggcttcatca ccgggcagaa ccttgtggcc gatggcggtg cctatccggg
     2641 gacgctgtag agcatgctgc gcaaaagtgt gaacggtttt tcgccgaaag catgctctaa
     2701 actattagaa tcgatcacgt tgtctcttgt tttggtgttt ccaccaaaac gagagcgtga
     2761 tctagggtcc gcactgccgg cttgcccaga cgcgcgcttg ccgctaactc gaagctcggc
     2821 gcggacgtgg cgaaaccggt agacgcaagg gacttaaaat ccctcggagg caactctgtg
     2881 cgggttcgac ccccgccgtc cgcaccattc ttgaaaggac agcacgatgg ctgtgcgtga
     2941 tgccaacggc accgaactca aagacgggga ttcagtcacc ctgatcaagg acctgaaggt
     3001 caagggaacc tcggtgacgc tgaagcgtgg caccctgatc aagcaaatcc ggctcaccgc
     3061 cgatgatgag gagatcgagg ggcggagcga caaggtgaaa gggctcgtcc tgcgcaccga
     3121 attcgtcaaa cgcgcctgac gctgcatccc gcacactggg ttagagcatg ctgcgatcaa
     3181 gtggataccg gctaatcgca aaggcatgct ccaaaaacgg gagcgtgatc tagcggcatg
     3241 gacagcctga cagattatga cttcgatctg cccgatcgtc tgatcgcact ctaccccgca
     3301 cgcccgcgcg attcggcgcg gatgctggtg gtgaggccgg gccaggcgct cgagcatctc
     3361 ggcgtgcgcg atcttcctgc cctgctgcgc cccggcgatg cactggtgtt caacgatacc
     3421 cgcgtcattc cggcgcggct gcgcggcgtc cgccttcgcg gcggcggctc
//
LOCUS       DAOMXG010000215         3437 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_15214_length_3437_cov_11.193968, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000215 DAOMXG010000000
VERSION     DAOMXG010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15214_length_3437_cov_11.193968"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PLQ11_11500"
     CDS             <1..200
                     /locus_tag="PLQ11_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein"
                     /protein_id="HRK25567.1"
                     /translation="RRRRTRSRRSPTAQGQTPSGCVFRTRCPLATAACAAAIPPMTEA
                     GPGHTYACIHAAQAPASALPG"
     gene            212..1150
                     /locus_tag="PLQ11_11505"
     CDS             212..1150
                     /locus_tag="PLQ11_11505"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRK25568.1"
                     /translation="MSEPFHDPDAGQSVEARAGNILLTIRTLLPDLSKAHRQIAEMVL
                     ADPGWSVQSNVEDLAARAGVAKPTIVRFARAVGCEGLKDFKLKLAGSLALGSSYLHRA
                     VRASDTPGEVINNVVGSTLAALAEWHRMLDPDRLNEAANVLNRARRVDCFGTGATSHF
                     LAQDLQARLFRLGLTATSYTDAYLQLVAAATLARDDAVVAISFVGRMPALLEAVAMAK
                     ARNARIIAITRANTPLARLADIVLAVDVPADATMPVGTDAYITQTMMIEMITILLGRL
                     RGPECVKRLEQIHRILQAKDQETDESSVVYWGWKSS"
     gene            1181..1576
                     /locus_tag="PLQ11_11510"
     CDS             1181..1576
                     /locus_tag="PLQ11_11510"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018065661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRK25569.1"
                     /translation="MTIEHLGGEGFGAARSAMPLSPAVRAGDFVYISGQVPTDERGEI
                     VWGNVETQTRVVMEKLKTALGYAGATLADVVKTTVYLADARDFVGFNGVYSSYFEPGK
                     RPARTTIQSPLILDIRVEIDAVAYLPKKR"
     gene            1579..2640
                     /locus_tag="PLQ11_11515"
     CDS             1579..2640
                     /locus_tag="PLQ11_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010915534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-TA family PLP-dependent enzyme"
                     /protein_id="HRK25570.1"
                     /translation="MSQVPQVETPGVLIDEDIVERNIGAAQAQFNRLGIALRPHIKTH
                     KLVRFAKKQIAAGAVGITCQKTSEAEAFVEAGCRDVLITFNILGEAKLARLRRLSANA
                     TVTVVADNATVVQGLSGAFAQAPQPLRVLVECDTGLGRCGVQSPEAAVALARLIARSP
                     GLAFAGLMTYPAPNSHERVEAFMVAAKAGCLAAVGSCETISSGGTPSMRSVPDKTVIT
                     EYRPGTYIYNDRSLIARGACTLDDCALAVAATVVSRPTPDRAILDAGSKTLSSDLLGL
                     TGYGLLRDYPDAAIKALSEEHGHVDLSACAARPEVGETVLIIPNHVCVVTNLFDQVHL
                     HRSGRFVETVRVDARGRVF"
     gene            2651..3349
                     /locus_tag="PLQ11_11520"
     CDS             2651..3349
                     /locus_tag="PLQ11_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008877408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HRK25571.1"
                     /translation="MPAKLPDNESDLFTLMRKELFSSVIGDVLDAQGFRHQYLPPNIR
                     PLSDDMIIIGRAMTVLEADFFVEPGMDGRSGLATKPFGVMFEALDDLRRNEIYITSGV
                     CGAYAQWGEMMSTRARVLGAAGAILNGYARDTHGIRKLGFPTFCHGSYGQDQGVRGKV
                     LDYRLRIEIGAVTIDPGTILFGDIDGVVAIPRAAEQETLRLAFEKARGEKRVAKAIGE
                     GMSTVEAWNKFGIM"
BASE COUNT          608 a         1095 c         1109 g          625 t
ORIGIN      
        1 aaaggcggcg tagaacaaga tccagaaggt ctcccacggc ccaagggcaa acaccttcgg
       61 gctgcgtgtt ccggacccgc tgtccgctgg caacggctgc atgtgccgcc gccattccgc
      121 cgatgaccga ggctggtccg ggccatacct acgcctgtat ccatgctgcg caggcgcccg
      181 cgtcggcgct gcccggctga aggatgcggc catgagcgag cctttccacg atccggatgc
      241 cggccagagc gttgaggcgc gcgccggcaa cattctgctg accatccgca cgctgttgcc
      301 ggacctttcc aaagcccacc ggcagattgc cgaaatggtg ctggccgatc ccggctggtc
      361 ggtgcagagc aatgtcgagg atctggcggc gcgcgccggt gtcgccaaac ccaccatcgt
      421 gcgttttgcc cgggcggtcg gctgcgaggg cctcaaggat ttcaagctga aactggccgg
      481 gtcactggcg ctgggctcat cctatctgca ccgcgcggtg cgcgcgtcgg atacgccggg
      541 cgaggtgatc aacaatgtgg tcggatccac tttggcggcg ctggccgaat ggcaccggat
      601 gctcgaccca gaccggttga acgaggctgc caatgtgctg aaccgggcac gccgggtcga
      661 ttgcttcggc accggggcga cgtcacattt tctggcccag gacctgcagg cccggttgtt
      721 ccgtctcgga ctgacggcga caagctatac cgacgcctat ctgcaactcg tcgcagccgc
      781 gacccttgcc cgggatgatg cggtggtcgc catttccttc gtcgggcgga tgccggcttt
      841 gctggaggcc gtggcgatgg ccaaggcccg caatgcccgg atcatcgcga tcacacgcgc
      901 caataccccg ctggcccggc tggcggatat cgtgctggcg gtcgatgttc cggccgatgc
      961 gaccatgccg gtgggcacgg atgcctatat cacccagacg atgatgatcg agatgatcac
     1021 catactgctc gggcgcctgc gcggcccgga gtgtgtgaag cggctggagc agatccacag
     1081 gatactccag gcaaaagatc aggaaacgga cgaaagctcg gtggtctatt ggggctggaa
     1141 aagctcatga aacaatcagg acacgatcgg agataaagcg atgacgattg aacaccttgg
     1201 gggtgaaggg tttggcgcgg cgcgcagtgc catgccgctg tcaccggcgg tccgcgccgg
     1261 cgattttgtc tacatttccg gacaggttcc taccgacgag cgcggcgaga tcgtctgggg
     1321 gaacgtcgag acacagaccc gggttgtgat ggagaagctc aagacagccc tcggctatgc
     1381 cggcgccacg ctggccgatg tggtcaagac caccgtctat cttgccgacg cacgcgattt
     1441 cgtcgggttc aacggcgttt attcgagcta tttcgaaccg ggcaaacgcc cggcccgcac
     1501 cacgatccag tcgccgctga tcctcgacat ccgcgtcgag atcgatgccg tggcctatct
     1561 gccgaagaag cgctgacgat gagccaggtc ccgcaggtcg aaacccccgg cgtgctgatc
     1621 gacgaggata tcgtcgagcg caacatcggc gcagcgcagg cgcagttcaa ccgcctcggc
     1681 atcgcgctgc gtccgcatat caagacccac aagctggtgc ggtttgccaa aaagcagatc
     1741 gctgccggcg cggtcggcat cacctgccag aaaacctccg aggccgaagc ctttgtcgag
     1801 gcgggctgtc gggatgtgct gatcaccttc aatatcctgg gcgaggccaa gctcgcccgc
     1861 ttgcgccgtc tcagcgcaaa cgcaacggtg acggtcgttg ccgacaatgc gaccgtcgtg
     1921 caggggctgt cgggcgcctt tgcccaggcg ccccagcctt tgcgcgtgct ggtcgaatgc
     1981 gacaccggcc ttggccggtg cggcgtgcaa tcaccggagg cggcggtggc gctcgcgcgc
     2041 ctcattgccc ggagcccggg cctcgccttt gccggtctga tgacctatcc ggcgccgaat
     2101 tcgcacgagc gcgtcgaagc gttcatggtg gcggcgaagg ccgggtgtct tgccgccgtc
     2161 ggctcctgcg agacgatcag ctcgggcgga acaccgtcga tgcgttcggt gccggacaag
     2221 acggtgatca ccgaataccg gcccggaacc tatatctaca acgaccgctc gctgatcgcg
     2281 cgcggcgcct gcacgctgga cgattgcgcg cttgccgttg ccgcaaccgt ggtcagccgg
     2341 ccgacgccgg accgggcgat ccttgatgct ggctccaaga cgctgagttc ggatctgctc
     2401 gggctgaccg gctacggcct gctgcgcgac tatcccgatg ccgcgatcaa ggcgctgagc
     2461 gaggaacacg gccatgtcga tctgtccgcc tgcgcagcca ggccggaggt tggcgaaacg
     2521 gtgctgatca tcccgaacca cgtctgcgtc gtcaccaatc tgttcgatca ggtgcatctg
     2581 caccgcagtg gccgtttcgt cgagaccgtg cgcgtggatg cgcgcggccg cgtgttctga
     2641 agggtgatcc atgccagcca agcttcccga caatgaaagc gatctcttca ccctgatgcg
     2701 caaggagctg ttctccagcg tcatcggcga tgtcctcgat gcgcaggggt tccgccacca
     2761 gtatcttccg cccaacatcc ggcccctgtc cgacgacatg atcatcatcg gccgcgccat
     2821 gactgttctg gaggccgact tcttcgtcga accgggaatg gacggtcgtt ccggactggc
     2881 aaccaagcca tttggcgtga tgttcgaggc gctcgacgac ctcaggcgga atgaaatcta
     2941 cattacatcc ggtgtctgtg gcgcctatgc gcaatggggc gagatgatgt cgacccgtgc
     3001 ccgcgttctt ggcgcggcgg gcgcaatcct gaacggctac gcacgcgata cccatggcat
     3061 ccgcaagctc ggtttcccga ccttctgcca tggctcctac ggccaggatc agggggtgcg
     3121 cggcaaggtg ctcgactacc ggctgcggat cgagatcggt gccgtgacga tcgatccggg
     3181 cacgatcctg ttcggcgaca tcgacggtgt agtggccatc cccagggcgg cggaacagga
     3241 gacgctgcgg cttgccttcg aaaaggcacg cggcgaaaag cgcgttgcca aggccatcgg
     3301 cgaaggcatg agcactgtcg aggcctggaa caagttcggc atcatgtagg ctttgccgcg
     3361 ctacgctttg tctttcgcgc tgtgatacca gtggccctca caaatgacct gccggtcatt
     3421 tcccgggccg tccctcg
//
LOCUS       DAOMXG010000216         3355 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_15742_length_3355_cov_10.226970, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000216 DAOMXG010000000
VERSION     DAOMXG010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15742_length_3355_cov_10.226970"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1408
                     /locus_tag="PLQ11_11525"
     CDS             <1..1408
                     /locus_tag="PLQ11_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRK25572.1"
                     /translation="VLSETPAYSDVQPWRLAMVLDVSEAGARIGLQPGREANGTLSKR
                     REIGLIPPDGVRWTRRSLRQVLNPGDVIYAEPVANTANNFRLRQVPDISGALVAMDPF
                     TGRILAMVGGFSFEMSEFNRATQALRQPGSSFKPFVYATALDNGYTPSSIIIDEPIEV
                     DQGGTLGIWRPENYDGKSTGPRTLRYGIEHSKNLMTVRLARDVGMPLIAEYSKRFGVY
                     DDMLPVLSMALGAGETTVLRMTAAYSMFANGGKRIKPTMIDRIQDRWGQTIYRHDERV
                     CQACKADEWREQDEPRLVDKREQVIDPMSAYQITSMMEGVVQRGTAQVIKDVGRTLAG
                     KTGTTNDAKDVWFVGFSPDLAVGIYLGFDKPKSLGDSATAGQYAAPIFRDFMKVALAG
                     KPDTKFRIPPQMKLIRVSVATGQRVGAGEGPGTILEAFKPGTAPPESVSFGSGKSGTG
                     AAAGGGAAAAAPTTGGLY"
     gene            1485..2616
                     /gene="prfB"
                     /locus_tag="PLQ11_11530"
     CDS             join(1485..1553,1555..2616)
                     /gene="prfB"
                     /locus_tag="PLQ11_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502756.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HRK25573.1"
                     /translation="MRAEHQALSEAIKQSVGLLRRHLDFDAATKRLAELNALSEEQDF
                     WNDATRAQKLMRERTELETRLNNLRKLESDLEDSITLIDLGEAEDDADTIAEGDAMLR
                     ALQQEAGRQQLDAMLSGEADSNDTYLEVHAGAGGTESQDWASMLLRMYSRWAERKGYK
                     IELMDQSDGEEAGIKSATLLIKGHNAYGWLKTEAGVHRLVRISPYDSNARRHTSFASV
                     TVYPVVDDAINIEINEADVRTDVMRSGGAGGQHVNKTESAVRLVHIPTGVMVVSQGDR
                     SQHKNRDQAWKMLRAKLYELELKKREEAAMAEQANKTDIGWGHQIRSYVLQPYQMVKD
                     LRTGVTSGVPQDVLDGALDPFMEATLAQRAYGGGPASVEDVE"
     gene            complement(2617..>3355)
                     /locus_tag="PLQ11_11535"
     CDS             complement(2617..>3355)
                     /locus_tag="PLQ11_11535"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRK25574.1"
                     /translation="IDRRSLPLLIEQAQASAHIMTNNQIALLRDIEASAQSASRQMKS
                     ALDLTGLDLTRFGKALNRQPAPAAAVGGPLIPLTKSESAEFERRLASAENQLRESERL
                     SRVVRALPVRRPLPRHHETTSTFGTRTDPFTRGLAMHSGLDFRATTGTPVRVTAEGKV
                     VEAGWVGGYGKMVEIDHGFGLTTRYGHLSGIDVAVGDTVRKGEIVGQVGSTGRSTGPH
                     LHYEVRIDDEAVDPQTLLRAGERAAIQ"
BASE COUNT          616 a         1057 c         1132 g          550 t
ORIGIN      
        1 ggtgctgtcg gaaacgccgg cctattcgga cgttcagccg tggcggctgg cgatggtgct
       61 cgacgtgtcg gaggccggcg cgcgcatcgg cttgcagccg gggcgcgagg ccaacggaac
      121 gctgtcgaag cgccgcgaga tcggcctgat cccccccgat ggcgtgcgct ggacgcggcg
      181 cagcctccgt caggtgctca atcccggcga tgtgatctac gccgagccgg ttgccaatac
      241 cgccaacaat ttccgtctgc ggcaggtgcc ggatatttcc ggcgcactgg tggcgatgga
      301 cccgttcacc gggcgcatcc tggccatggt gggcggattc tccttcgaga tgagcgagtt
      361 caaccgggcg acgcaggcgc tgcgccagcc gggttcgtca ttcaagccgt tcgtctatgc
      421 gacggcgctg gacaatggct atacgccctc gtcgatcatc atcgacgagc cgatcgaggt
      481 cgatcagggt ggcacgctcg ggatctggcg gccggaaaac tacgatggca agtcgaccgg
      541 gccacgcacc ctgcgctatg gcatcgagca ttccaagaat ctgatgacgg tgcggctggc
      601 gcgcgatgtc ggcatgccgt tgatcgccga atattcaaag cgtttcggcg tttatgacga
      661 tatgctgccg gtgctgtcga tggcgctggg tgcaggcgag accacggttc tgcgcatgac
      721 cgcggcctat tcgatgttcg ccaacggcgg caaacgcatc aagccgacga tgatcgaccg
      781 catccaggat cgctgggggc agacgatcta caggcatgac gagcgggtct gccaggcctg
      841 caaggccgac gagtggcgcg agcaggacga gccgcggctg gtcgacaagc gcgagcaggt
      901 gatcgatccg atgtcggcct atcagatcac ctcgatgatg gagggtgtgg tccagcgcgg
      961 caccgcccag gtcatcaagg atgtcggccg cacgcttgcc ggcaagaccg gcacgaccaa
     1021 cgacgccaag gacgtctggt tcgtcgggtt ctcgccggat ctggcagtcg gcatctacct
     1081 cgggttcgac aagcccaagt cgctgggcga ttcggcgact gccggccaat atgccgcgcc
     1141 gatcttccgc gatttcatga aggtggcgct ggccggcaag cccgatacca aattccgtat
     1201 cccgccgcag atgaagctga tccgggtctc ggtggcgacc ggccagcgcg tcggcgcagg
     1261 cgaggggccg ggcacgatcc tcgaagcctt caagccgggc acggccccgc cggagagcgt
     1321 cagcttcggc agcggcaaat ccgggacggg cgccgcagcg ggcggcggcg cggcagccgc
     1381 cgcgccaaca accggcggac tctactgaaa cagccgttta cagaagcgcg ccgggcggct
     1441 atcccccggc gttccttgca tttgctcaaa aggttcagca tcccatgcgc gccgaacatc
     1501 aggccctctc cgaggcgatc aagcagtcag tcgggctgct gaggaggcat ctttgacttc
     1561 gatgccgcta caaaacgtct ggccgagctg aacgcgctct ccgaagagca ggatttctgg
     1621 aacgatgcga cgcgggcgca gaagttgatg cgcgagcgca ccgagcttga aacccgtctc
     1681 aacaacctca ggaagctcga atccgacctt gaggattcga tcacgctgat cgacctcggc
     1741 gaagccgagg atgatgcgga tacgattgcc gagggcgatg ccatgctgcg cgcccttcag
     1801 caggaggccg gtcgccagca gctcgacgcg atgctgtcgg gcgaggccga ttccaacgac
     1861 acctatcttg aagtccatgc cggcgcgggc ggcaccgaga gccaggactg ggcctcgatg
     1921 ctgctgcgca tgtattcgcg ctgggccgag cgcaaaggct acaagatcga actgatggac
     1981 cagtcagacg gcgaggaagc gggcatcaag tcggcaacgc tgctgatcaa gggccacaac
     2041 gcctatggct ggctgaaaac cgaggccggc gtgcaccggc tggtgcgcat cagcccgtac
     2101 gattccaacg cgcggcgcca cacctccttc gcctcggtga cggtctatcc ggtggtcgac
     2161 gatgccatca acatcgagat caatgaggcc gacgtgcgca ccgacgtgat gcgctcgggc
     2221 ggcgccggcg gccagcacgt caacaagacc gaatctgccg tccgcctcgt gcatatcccg
     2281 accggtgtga tggtggtcag ccagggcgac cgttcgcagc acaagaaccg cgatcaggcc
     2341 tggaagatgc tgcgcgccaa gctttacgaa ctggaactga agaagcgcga ggaagcggcg
     2401 atggccgagc aggccaacaa gaccgatatc ggctggggcc accagatccg ctcctacgtg
     2461 ctgcaaccct accagatggt caaggacctc aggacaggcg tcacctccgg cgtgccgcag
     2521 gatgtgctcg acggcgcgct cgatccgttc atggaggcga cgctggccca gcgtgcctat
     2581 ggcggcggac cggccagtgt cgaggatgtg gagtagttat tggatcgcgg cgcgttcgcc
     2641 ggcgcgcagg agggtttgcg gatcgacggc ctcatcgtcg atgcgcacct cataatgcag
     2701 atgcgggccg gtcgagcggc cggtcgagcc gacctgaccg acgatttcgc ccttgcgcac
     2761 ggtatcgccc accgccacgt cgatgccgga cagatgaccg tagcgggtgg tcaggccgaa
     2821 gccatggtcg atctcgacca tcttgccata gccgccgacc cagccggcct cgacgacctt
     2881 gccttctgcg gtgacacgca cgggagttcc ggttgtggcg cgaaaatcca gcccggaatg
     2941 catcgccagc ccgcgcgtga acggatcggt acgggttccg aacgtgctgg tggtttcatg
     3001 gtggcgaggc aacgggcgcc gcaccggtag ggcgcgcacc acacgcgaca ggcgctcgct
     3061 ctcgcgcaac tggttctcgg ccgatgcgag gcgccgctcg aactcggccg attccgattt
     3121 cgtcagcgga ataagcgggc cgccgacagc ggctgccggc gcgggctggc gattcaacgc
     3181 cttgccgaaa cgggtcaggt cgaggccggt cagatccagc gccgacttca tctggcggct
     3241 ggcactctgg gcgctggcct cgatgtcgcg cagcagcgcg atctgattgt tggtcatgat
     3301 atgggcgctg gcctgagcct gctcgatcag cagcggcagg ctgcggcggt cgatc
//
LOCUS       DAOMXG010000217         3353 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_15760_length_3353_cov_8.320800, whole genome shotgun sequence.
ACCESSION   DAOMXG010000217 DAOMXG010000000
VERSION     DAOMXG010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15760_length_3353_cov_8.320800"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..1214)
                     /locus_tag="PLQ11_11540"
     CDS             complement(21..1214)
                     /locus_tag="PLQ11_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008548131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRK25575.1"
                     /translation="MSPALRSPLLFLMAINFINLLGYAGWNGLFNNFSKEMAGFTGYE
                     IGIAQSVREIPGFLAFTAIVWFLVMREQVFALVSLIVLGAGCVLTGFLPSFWGILIST
                     TVMSVGFHYSETVNQSLGLQLFSKKDAPRMLGKVAGAGAAAQLVGYGSMALLWWSGLR
                     DYQILYGLLGGVCMAAALLCWIWFPRVEGTVPQRKTLVIRSRYWLFYALTLMSGARRQ
                     IFSAFAAFLLVERFGMKVHEVAGLFLATAALNILLAPWFGALIARIGERRTIMLENLV
                     LIVVFAGYATTDDPLIAAVLFVLDGVFMTLVIAQRTYFQKIGDAADMSPTAAVSFTIN
                     HIAAVFIPFVFGLLWIKDPSLVFQAGGLIATISLTLAFLVPRDPGPGRETVLLESAAA
                     AATPA"
     gene            1364..1786
                     /locus_tag="PLQ11_11545"
     CDS             1364..1786
                     /locus_tag="PLQ11_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRK25576.1"
                     /translation="MYTRILVPVDLGDLKVARPAISRAVAMAKASGAAVRLLNVLSLI
                     PAAYLDYMPTNFDTQERQRAEEDMAALVKETGLAADKVSTVVRIGAIYHEVLSEASDW
                     KADLIVVGSHDPSFATYLIGSNAANIVRHAHCSVLVVR"
     gene            complement(1835..2473)
                     /locus_tag="PLQ11_11550"
     CDS             complement(1835..2473)
                     /locus_tag="PLQ11_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25577.1"
                     /translation="MRLPIVALAGRCLPCDGTIMTQRIRYAFRPRPFAAETVLDLTDR
                     AIEAQRAGKDSTIALRDIVTIRLFYAPRGINFTGFRAKLYTRGGTTLAFEDRSFKGLV
                     EQERLEAAYRAFVSELCSRAEAANPNVLLYGGRAVTILALVGGVGLLTTNLLAYFVLR
                     SVMEGKVLLAAGIAVFVGWFAAWTWAYVMRNRPQSFRAAAIPPQVLPSPDAG"
     gene            2571..3098
                     /locus_tag="PLQ11_11555"
     CDS             2571..3098
                     /locus_tag="PLQ11_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRK25578.1"
                     /translation="MKTLIVLRHAKAVPAGPSGDYQRVLADAGRHAATLIGADFKKHG
                     LKPEVLLVSSAARTQETFRIAAAEAGLGEASIEDDLYLAAAGVLLRRLRKVPPRTKSV
                     LLVGHNPGVAELVQKLADPSESDAAALSKARARFPTAACAVLEVLTPWSELQDGDCAL
                     KRYVTPADLGGANEE"
     gene            3100..>3353
                     /locus_tag="PLQ11_11560"
     CDS             3100..>3353
                     /locus_tag="PLQ11_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HRK25579.1"
                     /translation="MASDSLTGWTEAARGASASLFDYLSSFSRPNLRIGVTGLSRAGK
                     TVFITALVHALIGGGRLPVFRALSEGRISRARIAEQPDAAV"
BASE COUNT          651 a         1039 c         1124 g          539 t
ORIGIN      
        1 gcagggctga acggccggca tcaggctggc gtggccgccg ccgcagcgct ctcgagcagg
       61 acggtttcgc gccccggtcc gggatcgcgc ggcaccagga aggccagcgt cagcgagatc
      121 gtggcgatca acccgcccgc ctggaacacc agcgacgggt ccttgatcca caacaggcca
      181 aagacaaacg gaatgaacac cgccgcgatg tggttgatgg tgaaggagac ggccgccgtc
      241 ggcgacatgt ccgccgcatc gccgatcttc tggaaatagg tgcgctgggc aatcaccagc
      301 gtcatgaaaa ccccgtcaag cacaaacagg acggcggcaa tcagcggatc gtcggtcgtg
      361 gcatagccgg caaaaacgac gatcagcacc aggttttcca gcatgatggt gcgccgctcg
      421 ccgatcctgg cgatcagggc gccgaaccag ggcgccagca ggatgttgag cgccgcagtg
      481 gccaggaaca gaccggccac ctcatgcacc ttcatgccga aacgctcaac cagcagaaag
      541 gcggcgaatg ccgagaaaat ctgccgtcgg gcgccgctca tcagggtcag ggcatagaac
      601 agccagtagc gtgagcggat gaccagtgtc ttgcgctgcg gcaccgtgcc ctcgacccgc
      661 ggaaaccaga tccagcaaag caaggccgcc gccatgcaga cgccgccaag caggccgtag
      721 agaatttgat aatcgcgcag gcccgaccac catagcagcg ccatgctgcc gtatccgacc
      781 agttgggcgg cggcaccggc gccggcgacc ttgccgagca tgcgcggcgc gtccttcttg
      841 gagaaaagtt gcaggccgag cgactggttg accgtttcgg aatagtggaa gccgacggac
      901 atcaccgtgg tcgagatcag gatgccccag aacgagggca gaaaaccggt caggacacag
      961 ccggcgccca gcacgatcag gctgacaagc gcgaacacct gttcgcgcat caccagaaac
     1021 cagacgatgg cggtaaacgc cagaaagccg gggatttcac ggacgctctg cgcgatgccg
     1081 atctcgtagc cggtgaaacc ggccatctcc ttggaaaagt tgttgaacag gccgttccag
     1141 ccggcataac caagcagatt gatgaagttg atcgccatca ggaacagcag cggcgagcgc
     1201 agggcgggtg acatcgttgc agacctccgg cgggtgcccg tccgtgcttg tgctctgtgg
     1261 cgaagccggc gtcaatctcg gcgagcctgt tgaggattgc tctgccgcaa tgacagtgtc
     1321 atggggcgat tctagcattc cgatccacag tcaggagggg acgatgtata ctcgcattct
     1381 ggttccggtc gatctcggtg acctcaaggt ggcgcggccc gccatcagcc gcgcggtcgc
     1441 catggccaaa gcctcgggcg cggcggttcg cctgctcaat gtgctgtcgc tgatcccggc
     1501 ggcctatctc gactacatgc cgaccaattt cgacacacag gagcgccagc gcgccgaaga
     1561 ggatatggcg gcgctggtca aggagaccgg actggcggca gacaaggtgt cgacagtggt
     1621 gcggatcggg gcgatctacc acgaggtgct gagcgaagct tccgactgga aggccgacct
     1681 catcgtggtc gggtcgcatg atccgagctt tgccacctat ctgatcggtt cgaacgccgc
     1741 caacatcgtc cgccacgccc attgctcggt gctggtggtg cgctgaagtc attctttggc
     1801 gaagcggcgg tctccaggcc ggtggccgct gccgtcatcc cgcgtcgggc gagggcagga
     1861 cctgcggcgg gatggccgcg gcccggaaac tctgcggccg gttgcgcatc acataggccc
     1921 aggtccaggc tgcaaaccag ccgacgaaaa ccgcgatgcc ggcggccagc agcaccttgc
     1981 cttccatcac cgagcgcagg acgaaatagg cgagcaggtt ggtcgtcagc aggccgacgc
     2041 cgccgaccag cgccagaatt gtcaccgcac ggccgccgta aagcagtacg ttcgggttgg
     2101 cggcctcggc gcggctgcag agttcgctga caaaggcgcg ataggccgct tccagccgct
     2161 cctgctcgac cagccctttg aaactacggt cctcgaaggc cagggtcgtg ccgccgcgtg
     2221 tataaagctt ggcgcggaaa ccggtgaaat tgatgccgcg cggagcatag aacaggcgga
     2281 tggtgacgat atcgcgcagg gcgatggtcg aatccttgcc ggcccgctgg gcctcgatgg
     2341 cgcgatcggt gagatccagc acggtctcgg cggcaaaggg ccgggggcgg aaagcatagc
     2401 ggatccgttg ggtcatgatc gttccgtcgc atggcaggca gcggcctgca agtgccacga
     2461 tcggcagtcg catgggtcga tcccttgaga taatcccgac aatcgaccga gtgcccctcg
     2521 caattttttg ctcgcgatgg cagatagagg cggatagcag acagggaatc atgaaaacac
     2581 tgattgtttt gcggcacgcc aaggccgtgc cggctggccc atccggcgat tatcagcgcg
     2641 ttctggctga tgccgggcgc cacgcagcga cgctgatcgg cgccgacttc aaaaagcatg
     2701 ggctcaagcc cgaggtgctg ctggtttcca gcgcggcgcg gacccaggag acgttccgca
     2761 tcgctgcggc agaagccggg ctgggcgaag cgtcgatcga ggacgatctt tatctggccg
     2821 cggcgggcgt gttgctgcgg cgactgcgca aagtgccgcc gcgcaccaaa tcggtgctgc
     2881 tggtcgggca taatccgggc gttgccgagc ttgtgcagaa gctggccgat ccgtcggaga
     2941 gcgatgctgc ggcgctgtcc aaggcgagag cccgctttcc caccgccgcc tgcgccgttc
     3001 tcgaggtcct gaccccgtgg agcgagttgc aggatggcga ttgcgcgctc aagcgctacg
     3061 tgacgccagc cgatcttggc ggcgccaacg aggaatgatg tggccagtga cagtttgaca
     3121 ggatggacgg aggccgcgcg cggcgcctcg gccagcctgt tcgactacct ttccagcttt
     3181 tctcggccga atctccgcat cggcgtcacc ggcctgtcgc gggccggcaa gacggtgttc
     3241 atcaccgcgc tggtgcatgc gctgatcggc ggcgggcggt tgccggtgtt ccgggcgctg
     3301 agcgagggcc gcatcagccg cgcgcgcatc gccgaacagc ccgatgcggc ggt
//
LOCUS       DAOMXG010000218         3332 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_15887_length_3332_cov_8.068660, whole genome shotgun sequence.
ACCESSION   DAOMXG010000218 DAOMXG010000000
VERSION     DAOMXG010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15887_length_3332_cov_8.068660"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1173)
                     /locus_tag="PLQ11_11565"
     CDS             complement(<1..1173)
                     /locus_tag="PLQ11_11565"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRK25580.1"
                     /translation="MTNFSSLGLAEPLLRALETEGYTTPTPIQAKAIPLLMEGHDLLG
                     IAQTGTGKTAAFALPILNRLLAEKRRPTPGTARVLVLAPTRELAAQIGESFQAYARFC
                     RFSVAVVFGGVGYVPQTKAIRAGLDVLVATPGRLIDHLSDGVLKLHETTCVVLDEADH
                     MLDLGFVVPIRKILAKLPKQRQSLFFSATMPKEIATLAGEMLKNPKEVAVAPVATTAE
                     RVNQQVYLVDAAAKRGLLVEMLEKPSFERTIVFTRTKRGADRVSKTLEAVGIQSAPIH
                     GNKSQTQRQRALDDFKAGRIRVLVATDIAARGIDIDSVTHVINFELPEVPEQYVHRIG
                     RTARAGASGAAIAFCDASERGLLRDIERVTRQQIPTIDKRGHKGREVPAAQDVRPDD"
     gene            complement(1393..1842)
                     /locus_tag="PLQ11_11570"
     CDS             complement(1393..1842)
                     /locus_tag="PLQ11_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012091943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1489 family protein"
                     /protein_id="HRK25581.1"
                     /translation="MTLHLLKLCVGVESIDDLAEWIDEQRLYARRAGQNFEQKHTTRM
                     VPRRLDEILRAPDGPGSLYWVIKGQIACRQRLIDIRPFTDAHGIGRCHLVLEPEVVPV
                     LPRPYRAFQGWRYFSPEDAPPDLGARVEGLDTMPEALRRELAGLGLI"
     gene            complement(1839..2183)
                     /locus_tag="PLQ11_11575"
     CDS             complement(1839..2183)
                     /locus_tag="PLQ11_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25582.1"
                     /translation="MRSPLALMILLAAAPALACSCAPRNPAAPGPEVLVTAQVLTVTP
                     GPTAGTHVATLRVLRRHAGSVPRIIRVGSRSHSVACGVDFTKGETRQFALYRARGRLT
                     SNACYMFQHTRP"
     gene            complement(2183..>3332)
                     /locus_tag="PLQ11_11580"
     CDS             complement(2183..>3332)
                     /locus_tag="PLQ11_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase subunit L"
                     /protein_id="HRK25583.1"
                     /translation="ENAFYFMEMNTRLQVEHPVTEAITGLDLVELQFRVASGEKLPFA
                     QKDLKIHGHAVEARLYAEDPESGFLPSTGTLWALRLPEDGVRVDTGVAEGGAVTGFYD
                     PMIAKIITHGETRDAALDRLAAALGETLVAGPRTNVRFLKALCEAPGFRAGDFDTGFI
                     DRNLDGLGAVVQPLDLDAVAAGAALLIEQALDQTWAGPSSDPWNVRDGFALSGSTLPR
                     IEVAAGGERLVAQVEEDGWRSYRIRLAGDAAPRPIALEETEADGLDIVNDDAGFVAVT
                     TDDGVLALRNGRQTEVRRHDPLAVDFDALDVPEGGALIKAPMHGKLIALYVKPGDVVA
                     KGARLAVVEAMKMEHALTAPRDGLVVEVASHVGQQVSEGAKLVVIGEG"
BASE COUNT          592 a         1082 c         1111 g          547 t
ORIGIN      
        1 gtcgtccggg cgcacgtcct gcgcggcggg aacctcgcgc cccttgtggc cgcgcttgtc
       61 gatggtgggg atctgctggc gcgtcacccg ctcgatgtca cgcagcaggc cgcgctcgga
      121 ggcatcgcag aaggcgattg cggcgcccga ggcaccggcc cgtgccgtgc ggccgatgcg
      181 gtggacatat tgttccggaa cctcgggcag ttcgaaattg atgacatggg tgacgctgtc
      241 gatgtcgatg ccgcgggcgg caatgtcggt ggccaccagc acgcggatgc gcccggcctt
      301 gaaatcatcg agtgcgcgct ggcgctgggt ctggctcttg ttgccatgga tgggggccga
      361 ctggatcccg acagcctcca gcgtcttgga aacccgatcc gccccccgct tggtgcgggt
      421 gaaaacaatg gtgcgttcga aggacggctt ttcgagcatt tcgacaagca ggccacgctt
      481 ggcggcggca tcgaccagat agacctgctg attgacccgc tcggccgtgg tggcgaccgg
      541 cgcgaccgcg acttccttcg ggttcttcag catttcgccg gccagagtgg cgatttcttt
      601 cggcatggtg gcagagaaga acaggctctg gcgctgcttc ggcagtttgg ccaggatctt
      661 gcggatcggc acgacaaagc cgaggtcgag catgtgatcg gcctcgtcga gaacgacgca
      721 ggtggtttca tgcagcttga gcacgccatc gctgagatgg tcgatcagcc ggccgggcgt
      781 ggccaccagc acatcgagcc cggcgcggat ggccttggtc tggggcacat aaccgacgcc
      841 gccgaacacg accgcaaccg aaaaccggca gaagcgggca taggcctgga agctctcgcc
      901 gatctgcgcc gccagctcac gggttggcgc cagcaccaga acgcgggctg tccccggagt
      961 cgggcggcgt ttttcggcca gcagcctgtt gaggatcggc agggcgaagg ccgccgtctt
     1021 gccggtgccg gtctgggcga tgccgagaag atcatggcct tccatcagca gcgggatggc
     1081 cttggcctgg atcggcgtgg gggtggtata gccctcggtt tcgagagcgc gaagcagagg
     1141 ctcggcaaga ccgagcgagg agaaattcgt caaaagactg tctcgtcaaa aagccatggt
     1201 ccgcatgccg atggcgcacg gattgaggcg ggggaaagga cgggccccgc gtaatttggg
     1261 ctgtcggatg tcaggatggt caggtgctcg acagaagaac cgggaaaccc ggcttacgcg
     1321 cctggagcac aggatttgct gcaacgcagc gaagcggctt atacgtggct ttgctgcgga
     1381 aagcaaccgg catcagatca gcccgagtcc ggcaagttcg cgccgcagtg cctcgggcat
     1441 cgtgtccagc ccttcgacgc gggcgccgag atcgggcgga gcgtcctccg gcgaaaagta
     1501 gcgccagccc tgaaaggccc ggtagggccg tggcaaaacc ggcaccacct cgggctccag
     1561 caccagatgg cagcggccga tgccgtgcgc gtcggtgaag gggcggatgt cgatcagccg
     1621 ctgccggcag gcgatctgtc ccttgatcac ccagtagagc gagccggggc cgtcgggcgc
     1681 gcgcaggatc tcgtccaggc gcctggggac catgcgggtc gtgtgcttct gctcaaagtt
     1741 ctgtccggcc cgccgggcat agagccgctg ctcgtcgatc cactcggcaa gatcgtcgat
     1801 cgattcgact ccgacgcaga gcttgagcag atgcagggtc atggccttgt gtgctggaac
     1861 atgtagcagg cattgctggt cagcctgccg cgcgcccggt agagcgcgaa ctggcgcgtc
     1921 tctcccttgg tgaagtcgac gccgcaggcg accgaatggg aacggcttcc cacgcggatg
     1981 atacgcggca ccgagccggc atggcgcctg agcacgcgca gcgttgcgac atgggtgccg
     2041 gccgtagggc ccggcgtgac ggtgagaacc tgtgccgtaa ccagcacctc ggggccgggc
     2101 gccgcaggat ttcgcggcgc gcaggagcag gcaagcgccg gcgccgccgc cagcagaatc
     2161 atcagcgcaa ggggcgagcg catcagcctt ctccgatcac caccagcttg gcgccttcgc
     2221 tgacctgctg gccgacatgg ctggccactt cgacgacaag accatcgcgc ggagcggtga
     2281 gcgcatgttc catcttcatc gcttccacca cggccagccg cgcgcccttg gccacgacat
     2341 cgccgggttt gacatagagc gcgatcagct tgccatgcat cggcgctttg atcagcgccc
     2401 cgccttccgg cacgtcgagc gcgtcgaaat ccaccgccag cggatcgtgg cgccgaacct
     2461 cggtctgccg gccattgcgc agcgccagca cgccatcatc cgttgtcacg gcgacgaaac
     2521 cggcatcatc attgacgatg tcgagaccat cggcctccgt ctcctcaagc gcgatcggcc
     2581 gcggcgcagc atcacccgcc agacggatcc gatagctgcg ccagccatcc tcttccacct
     2641 gggcgacaag tctttccccg ccggccgcca cctcgatgcg gggcagggtg ctgccggaga
     2701 gggcaaaacc gtcgcgcaca ttccatggat cgctggaggg ccccgcccag gtctggtcaa
     2761 gcgcctgctc gatcagcagc gccgcccccg ctgccaccgc atcaaggtca agcggctgga
     2821 ccacggcccc gagcccatca aggttgcgat caatgaaccc ggtgtcgaaa tccccggcac
     2881 ggaagcccgg cgcctcgcac agcgccttga gaaagcggac gttggtgcgc ggaccggcaa
     2941 ccagcgtctc gcccagcgcg gcggcgagac ggtcgagcgc ggcatcgcgc gtctcgccat
     3001 gggtgatgat cttggcgatc atcggatcat aaaagccggt gaccgcgccg ccttcggcaa
     3061 cgccggtatc gacgcgcacg ccatcctccg gcaggcgcag cgcccacagc gtcccggtgg
     3121 acggcaggaa gccgctctcg ggatcctcgg cataaagccg cgcctcgacc gcatggccgt
     3181 ggattttcag atccttctga gcgaagggca gcttttcgcc cgaggcaacg cggaactgca
     3241 attccaccag atcaagcccc gtaatggctt ccgtaaccgg gtgctccact tgaagacgcg
     3301 tattcatctc catgaagtaa aacgcatttt cc
//
LOCUS       DAOMXG010000219         3260 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_16389_length_3260_cov_10.064899, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000219 DAOMXG010000000
VERSION     DAOMXG010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16389_length_3260_cov_10.064899"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /gene="tolA"
                     /locus_tag="PLQ11_11585"
     CDS             <1..865
                     /gene="tolA"
                     /locus_tag="PLQ11_11585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HRK25584.1"
                     /translation="APPKPEPVAVPPKPAMPKAQPDEEDEDEAELIRQKQLKQKEAEQ
                     KAEEARKAEEKRKAEEARKAEEKRKADEARRLAEDKRKAEEKAELEEARKLAAEQEKA
                     EEKKRLADEAAKKAAEAARKAAEAKKAEEARKAAEKAESDKLNAAIRNRLLASRDAPA
                     SSGSTGPQVTRTASAGAPNATGARLSPSDREALMGLIKEQMERCWSVSGMSNPTVKPL
                     VRLQLTPSGAISVQPALVNNSADPAFRAIADSGMRAIRACAPYRIPSKFADKFEDWKT
                     ITVKLDPSDLL"
     gene            880..2205
                     /gene="tolB"
                     /locus_tag="PLQ11_11590"
     CDS             880..2205
                     /gene="tolB"
                     /locus_tag="PLQ11_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HRK25585.1"
                     /translation="MTRLVQMPLAPLLLPRRRFLAGAAAFGMFGASAARAELQITLSG
                     GDFKPMPIAIAEFGGEAQVASVIANNLTRSGLFALLDKARHPEKPAFDAQPNFPAWQA
                     AGVQALVTGRALRDGNRIRAEFRLWDIGAGAQSVGQQYSTDANAWRRVGHMISDAIYS
                     KVTGEKGFFDTRIVYVDESGPKNNRKKRLAIMDQDGANHKILSTGDALALTPRFSPSS
                     QEITYMSFVDGAEPRVFLVNLQSGKREAVGNFPGMSFSPRFSPDGNSVIMSLQQGGNA
                     NIYVMSLNSQEMRRLTNTAAIDTGPSFSPDGGRIVFESDRGGGQQIYIMDASGGGQKR
                     ISFGEGRYGTPVWSPKGDYIAFTRQKGGSFAIGIMKPDGSGERILTEGFHNEGPTWAP
                     NGRYLMYFSDQGGGPKLTMVDISGRVKVPVPTPGFGSDPAWSPLLGETK"
     gene            2339..2860
                     /gene="pal"
                     /locus_tag="PLQ11_11595"
     CDS             2339..2860
                     /gene="pal"
                     /locus_tag="PLQ11_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HRK25586.1"
                     /translation="MLCNVLSPKAAKFAAVALVALSLGACASKPEDQDGIFGGGSGRG
                     GQAGAAVPGSTQDFSVNVGDRVFFETDSTELTPTGVATLDKQANWLNRYPRYSFTVEG
                     HADERGTREYNFALSARRSETVRNYLAAKGVAANRMRTMSYGKERPVATCDDISCWSQ
                     NRRAVTVLSGAGS"
     gene            2993..>3260
                     /locus_tag="PLQ11_11600"
     CDS             2993..>3260
                     /locus_tag="PLQ11_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgF trimerization domain-containing protein"
                     /protein_id="HRK25587.1"
                     /translation="MPRLIAALFVAVFVLPFGAGAPARAQTDAAELLLRLDRLEGENR
                     RLNGQVEQMGHQIRRLEDQLKRFQSDVDFRFKESGGKPLPAPEKY"
BASE COUNT          619 a         1020 c         1078 g          543 t
ORIGIN      
        1 tgcaccgccc aaacccgagc cggtagccgt gccgcccaag ccagcgatgc cgaaagccca
       61 gcccgatgaa gaggatgagg acgaggccga actgatccgc cagaaacagc tgaagcagaa
      121 ggaagccgag cagaaggcag aggaagcgcg caaggcggag gaaaagcgca aagccgagga
      181 agcccgcaag gccgaggaaa agcgcaaggc cgacgaagcc cgccgcctcg ccgaggacaa
      241 gcgcaaggcc gaggaaaagg ccgagcttga ggaagcgcgc aaactcgccg ccgagcagga
      301 aaaggccgag gagaaaaaac gcctggctga cgaggcggcc aagaaggcag ccgaagcggc
      361 ccgcaaggcg gccgaggcga agaaggccga agaggcacgc aaggccgcag agaaggccga
      421 atccgacaag ctcaatgccg ccatccgcaa ccggcttctg gcgtcgcgcg atgccccggc
      481 ctcgtccggc tcgaccggtc cgcaggtgac tcggaccgcc tcggccggtg cgccgaatgc
      541 caccggagcg cgcctgtcgc cgtccgatcg cgaggcgctg atggggctga tcaaggaaca
      601 gatggagcgg tgctggagcg tgtcgggcat gagcaatccg accgtcaaac ctttggtgcg
      661 gctccagctt acgcccagcg gcgcgatttc ggtgcagccg gcgctggtca ataattccgc
      721 cgatccggcg ttccgcgcca tcgccgacag cggcatgcgg gcgatccgcg cctgtgcgcc
      781 ctaccgcatc ccgtccaagt tcgccgacaa gttcgaggac tggaaaacca tcaccgtcaa
      841 gctcgatccg agcgatctat tgtaacaaag agccgtgcca tgacccgtct tgtccagatg
      901 ccactcgctc cgctcttgct gccacgtcgc cggtttctgg cgggcgccgc cgctttcggc
      961 atgttcggag cctctgcagc gcgcgcggaa ttgcagatca ctctgtcggg gggtgatttc
     1021 aagccgatgc ccattgccat cgccgaattc ggcggcgagg cgcaggtggc cagtgtcatt
     1081 gccaacaatc tcacgcgctc gggccttttt gcgctgctcg acaaggcgcg gcacccggaa
     1141 aagccggctt tcgatgccca gccgaacttt cccgcctggc aggcggcggg cgtgcaggcg
     1201 ctggtgaccg gccgggcctt gcgcgacggc aaccgcatcc gcgccgagtt ccggctgtgg
     1261 gatatcggtg ccggggcgca atcggtcggg cagcaatatt ccaccgatgc caatgcctgg
     1321 cgccgggttg gccacatgat ctcggatgcg atctactcca aggtgaccgg cgaaaagggc
     1381 tttttcgata cccggatcgt ctatgtcgac gagagcgggc ccaagaacaa ccgcaagaaa
     1441 cgcctcgcca tcatggatca ggacggggcc aatcataaga tcctgtcgac gggcgatgcg
     1501 ctggcgctga cgccgcggtt ctcgccgtcc agccaggaaa tcacctacat gtcgttcgtc
     1561 gacggggcgg agcctcgcgt ctttctggtc aacctgcaga gcggcaagcg cgaggcggtg
     1621 ggcaatttcc ccggcatgag cttctcgccg cgcttctcgc cggatggcaa ctcggtcatc
     1681 atgagcctgc agcagggcgg caatgccaac atctatgtga tgagcctcaa ttcgcaggag
     1741 atgcggcggc tgaccaatac ggcggcgatc gacaccggtc cgtcgttctc tccggatggc
     1801 gggcgtatcg tgttcgaaag cgatcgcggc ggcggccagc agatctatat catggatgcc
     1861 tccggaggcg ggcagaagcg catctcgttc ggcgaagggc gctatggcac gccggtctgg
     1921 tcgccgaagg gcgattacat cgccttcacc cggcagaagg gcgggtcttt tgccatcgga
     1981 atcatgaagc cggacggttc gggcgagcga atcctcaccg aaggtttcca caatgagggt
     2041 ccgacctggg cgccgaacgg gcgctacctg atgtattttt ccgatcaggg cggcgggccg
     2101 aagctgacca tggtcgatat ctcgggccgg gtgaaggtgc cggtgccaac gccgggcttc
     2161 ggctcggatc cggcctggtc gccgctgctg ggcgagacca agtagcggtt tgttaaccaa
     2221 acgccacaat gccgccattt cgacattgtt tggcaacacc gaggtaaggt tgacggggca
     2281 ttgctggtgc tgccagcggg gcttgcccgc gccagaccct cgtcaggagc taacccggat
     2341 gctttgcaac gttctgtcgc ccaaggccgc caaattcgca gccgtggcgc tggtcgccct
     2401 ttcccttgga gcctgtgcca gcaagcccga agatcaggac gggatttttg gcgggggcag
     2461 tggtcgtggc gggcaggccg gcgcggccgt gcccggttca acccaggatt tttcggtcaa
     2521 tgtcggggac cgggtgttct ttgaaaccga ttcgaccgaa ctgaccccga ccggcgttgc
     2581 cacgctcgac aagcaggcca actggctgaa ccgctatccg cgttattctt tcaccgtcga
     2641 gggccatgcc gacgagcgcg gcacgcgcga atacaatttt gcgctgtcgg cgcgtcgatc
     2701 ggaaacggtg cgcaactacc tcgccgccaa gggggttgcc gccaaccgca tgcgcaccat
     2761 gtcctatggc aaggagcggc cagtggcgac ctgcgacgac atttcctgct ggagccagaa
     2821 ccgccgcgcg gtcaccgtgc tgtcgggcgc cgggtcgtag gtcgtcaaga caggtcgttc
     2881 cgttcatagt ttggccggaa tggtgcggtt atgatagagc gtcgtgcgtc cggctccatc
     2941 cggccgcaca gtgctcaaag acatcgccgc tgatgttcgc cacgggtctg ccatgccgcg
     3001 tctgattgct gccctcttcg tcgctgtctt tgtcctgccg tttggtgccg gcgcgccggc
     3061 gcgggcgcag accgatgcgg ccgagctgct gctgcggctt gatcggctgg agggagagaa
     3121 ccgccgcctc aacggtcagg tcgagcagat gggccatcag atccgcaggc tggaagacca
     3181 gctgaagcgc ttccagtcgg atgtcgactt ccgtttcaag gaaagcggcg gcaaaccgct
     3241 gccggcgccc gaaaaatatc
//
LOCUS       DAOMXG010000220         3239 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_16535_length_3239_cov_8.711055, whole genome shotgun sequence.
ACCESSION   DAOMXG010000220 DAOMXG010000000
VERSION     DAOMXG010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16535_length_3239_cov_8.711055"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1579
                     /locus_tag="PLQ11_11605"
     CDS             <1..1579
                     /locus_tag="PLQ11_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HRK25588.1"
                     /translation="VANIDEVIALIRGSADAATARESLMARAWPAADVAPLIALIDDP
                     RHGLSADGTYRLSERQAQAILDLRLQRLTALGRDEIAEELEKIAAEIRGFLAILSSRA
                     RILDIIKDELAAVRAAYASPRLTEIVDADSDFEDEDLIAREEMVVTVSHEGYVKRVPL
                     STYRAQRRGGKGRAGMQTRDEDFVTRLFVASTHAPVLFFSSLGQVYKMKVWKLPAATP
                     QGRGKALVNLLPLDPGERITTIMPLPEDEAQWERLDVMFATNTGSIRRNKLSDCVPRT
                     SLGKRVMEFDGDDQSIIDVALATESDDVLLTTARGQCIRFAVSDVRVFGGNGSTGTST
                     GVRGILLADGDRVISQSILRHAAATPEERAAYLKRRRAVAGDLTEDGPVPVDAEEATG
                     DSELGQERYAEMSAAEQFVLTVSVNGFGKRSSSFEYRITNRGGKGIVAMAVNERNGDL
                     VASFPVEHSDQIMLVTDGGQLIRCPVDGIRIAGRATQGVIVFNTADSDRVVSVERVSE
                     SEDDNDGSEAEDSDTDA"
     gene            1714..>3239
                     /locus_tag="PLQ11_11610"
     CDS             1714..>3239
                     /locus_tag="PLQ11_11610"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRK25589.1"
                     /translation="MVVSISPFRAFLTSLPARGTLALCAAVAVAVIAAAMLGYNELRD
                     GTARQISDRLDHSARAAGAAFESSRNLPLTARRSEGSRPVAFRIASGDGRQVLTPSAD
                     LDRLVEAAADVNQGDVHLFRFHAETGLFERIATSARGPGGEHVRTVHFGPQYPAYETL
                     QQLRPFRGAVPQFDRFRLAEFIPVVDDREHLAGALAVDIGWLDEVWRPTWDLVHRQVL
                     TLLVLLLGVAGLGVGLLLRAMQPLRLLSAYARRLARHEPVGAPPGLGRPDEIGDVSAG
                     LAELVVMRDRLEHLAFTDPLTGFANRARLEVMIGQQLEGSHRTPPDAALLLFGVDNFR
                     SLNDGFGFSVGDSLLREMAGRLQSLSPQGSLIARSGADEFAVLVPHCDSYAQMFDHAR
                     KLLAALRRPYAQAQGGVFASVSAGFAMLPASGGTAETVLRNATLALNRAKRQGRNRIE
                     MFSPCFDEEAQRRADLERNLNRAINSGELLLHFQPLVRTSDRRLIGVEALVRWDHPQL
                     G"
BASE COUNT          496 a         1030 c         1096 g          617 t
ORIGIN      
        1 ggtggccaat atcgacgagg tgatcgcgct gatccgcggt tcggccgatg ccgcgacggc
       61 gcgcgagtcg ctgatggcgc gggcctggcc ggcggccgat gtggcgccgc tgatcgcgct
      121 catcgacgat ccgcgccatg ggctttcggc cgatggcacc tatcggctat cggagcggca
      181 ggcgcaggcg atcctcgatc tcagattgca gcggctgacc gcgctcggtc gcgatgagat
      241 cgccgaggaa ctggaaaaga tcgcggcgga aattcgcggg tttcttgcca tcctgtcgtc
      301 gcgggcgcgc atcctcgaca tcatcaagga cgaactcgcc gccgtgcgtg ccgcctatgc
      361 atcaccgcgc ctgaccgaga tcgtcgacgc cgacagcgat ttcgaggacg aggatctgat
      421 cgcccgcgag gaaatggtcg tcacggtcag ccatgagggc tacgtcaagc gggtgccgct
      481 gtcgacctat cgcgcccagc gccgcggcgg caagggtcgc gccggcatgc agaccaggga
      541 cgaggatttc gtcacgcggc tgttcgttgc ctcgacccat gcgccggttc tgtttttctc
      601 gtcgctcggc caggtctaca agatgaaggt gtggaagctc ccggcagcga cgccgcaggg
      661 gcgcggcaag gcgctggtca acctgctgcc gctcgatccg ggcgagcgca tcaccacgat
      721 catgccgctg cccgaggacg aggcgcaatg ggagcggctg gacgtgatgt ttgccaccaa
      781 tacgggctcc atccgccgca acaagctcag cgattgcgtg ccgcgcacca gcctcggcaa
      841 gcgggtcatg gaatttgacg gggatgacca gtcgatcatc gatgtggctt tggccaccga
      901 gtccgatgac gtgctgctga ccacggcccg cggccagtgc atccgctttg ccgtgtcgga
      961 tgtgcgggtg ttcggcggca acggctcgac cggcacatcg accggtgtgc gcggcatcct
     1021 gctggctgac ggcgaccggg tgatctcgca gtcgatcctg cgccacgccg cggcaacgcc
     1081 ggaagagcgt gcagcctatc tcaagcggcg ccgcgcggtt gccggcgatc tgaccgagga
     1141 cgggccggtt ccggtcgatg ccgaggaggc gacgggcgac agcgaactcg gccaggagcg
     1201 ctatgcggaa atgtcggcgg ccgagcagtt cgtgctgacc gtatcggtca acggtttcgg
     1261 caagcgctcg tcctcgttcg agtatcggat caccaatcgc ggcggcaagg gcatcgtcgc
     1321 catggcggtc aacgagcgca atggcgatct cgtcgcctcc ttcccggtcg agcacagcga
     1381 ccagatcatg ttggtgaccg atggtggaca actgatccgc tgcccggtcg atggcatccg
     1441 tattgccggc cgggcgacgc agggcgtcat cgtcttcaac accgcggaca gcgaccgggt
     1501 ggtgtcggtc gagcgtgtct ccgagagcga ggatgacaac gacggcagcg aggctgagga
     1561 cagcgacact gacgcgtaga atttgaacat tctgctctgc ccggccttaa ccagccggac
     1621 agatccccgg tgcgctgacc ggattctgtt aaagcgatac gggttacggt ctcgcctgcc
     1681 gccgaggttt ttggggtggc gggagttgtt tcattggttg tttcaatctc gccgtttcgc
     1741 gcctttctga cctccctgcc cgcccgggga acgctggcgc tctgcgccgc ggtggcggtt
     1801 gccgtcatcg ccgctgccat gctcggctat aacgaattgc gcgatggcac ggcgcggcag
     1861 atctccgacc ggctcgatca ttcggcgcgt gccgcaggcg ctgcatttga atccagccgg
     1921 aacttgccgc tcaccgcgcg gcggtccgag ggcagtcgcc cggtggcctt ccgcattgcg
     1981 tcgggcgatg gccgccaggt gctgaccccg agtgccgacc tcgatcgcct ggtcgaagct
     2041 gcggctgacg tcaatcaggg cgatgttcac ctgttccgct ttcacgccga aacaggattg
     2101 ttcgagcgta ttgccaccag cgcgcgcggc cccggcggtg aacatgtgcg cacggtgcat
     2161 ttcggtccgc aatatccggc ctatgagaca ttgcagcagc tcaggccgtt ccgtggcgcg
     2221 gtgccgcaat ttgatcgttt ccggctcgcc gaattcattc cggttgtcga tgaccgggag
     2281 catttggccg gcgcactggc cgtcgatatc ggctggttgg acgaggtctg gcgtccgacc
     2341 tgggatctgg ttcatcgtca ggtgctgacg ttgctggtcc tgctgcttgg cgtggccggg
     2401 ctcggcgtcg ggctgttgct gagggcgatg cagccattgc gccttctgtc ggcctatgcc
     2461 cggcgccttg cgcgccatga acctgtcggc gcgccgcccg gccttggccg cccggatgag
     2521 atcggcgatg tttcggccgg gctggccgaa ctggtggtga tgcgcgacag gctcgagcac
     2581 ctggctttca ccgatccgct gaccggcttt gccaatcggg cccgtctgga ggtgatgatc
     2641 ggccagcagc tggaaggctc gcaccgaacc ccgccggacg cagcgctgct gctgtttggc
     2701 gtcgataatt tccgttcgct caatgatggc ttcggcttca gcgtcggcga tagcctgctt
     2761 cgggaaatgg cgggccggct ccagtcgctg tcgccgcagg gttcgctgat tgcccgcagc
     2821 ggcgccgacg aatttgccgt gctggtccct cactgcgaca gctatgccca gatgttcgat
     2881 cacgcccgca aactgcttgc ggctttgcgc cgaccctatg cgcaggcgca gggtggggtt
     2941 tttgccagcg tctcggccgg ctttgcgatg ctgccggcca gcggtgggac cgccgaaacg
     3001 gtactgcgca acgccacgct ggcgctcaat cgcgccaagc gccagggtcg caaccgcatc
     3061 gagatgttct cgccctgctt cgatgaggaa gcgcaacgtc gcgccgacct tgaacgcaat
     3121 ctcaaccggg ccatcaacag cggcgagttg ctgctgcatt tccagccgct ggtgcgcacc
     3181 tccgatcgcc gtctgatcgg tgtcgaggcg ctggtgcgct gggatcaccc tcagcttgg
//
LOCUS       DAOMXG010000221         3211 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_16759_length_3211_cov_9.106464, whole genome shotgun sequence.
ACCESSION   DAOMXG010000221 DAOMXG010000000
VERSION     DAOMXG010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16759_length_3211_cov_9.106464"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="PLQ11_11615"
     CDS             complement(<1..207)
                     /locus_tag="PLQ11_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3572 family protein"
                     /protein_id="HRK25590.1"
                     /translation="MARALPDPDPEALAIDALGFIAGDAERLGRFLALTGLGPGTLRA
                     AAADPAFLIRVLDYLAEDESALIAF"
     gene            327..701
                     /locus_tag="PLQ11_11620"
     CDS             327..701
                     /locus_tag="PLQ11_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018045229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRK25591.1"
                     /translation="MSKTVLIVEDNELNMKLFNDLLQAHGIKTLLTSSGVEAIELARR
                     HMPDLILMDIQLPEVSGIEVTKWLKDDEHLKRIPVIAITAFAMKGDEERIRQSGCEGY
                     LSKPISVGKFVQTVQSYLGAAR"
     gene            756..2132
                     /locus_tag="PLQ11_11625"
     CDS             756..2132
                     /locus_tag="PLQ11_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PleD family two-component system response
                     regulator"
                     /protein_id="HRK25592.1"
                     /translation="MTARVLIVDDLFPNIKLLETRLTAEYFDVLSAMNGMQAIEICES
                     GACDIVLLDVMMPGMDGFEVCRRLKSSPTTAHLPIVMVTALDQPSDRLKGLEAGADDF
                     LTKPVNDVALLTRVRSLARLKTLTDELRARALATPQFGQSDPLAAAAAEDGLNGRVLI
                     IDDRANSSERMASSLSQVHQVDVESDPQAALMRLSEGEHDMAIISLGLANFDALRLCS
                     QMRSLERTRNTPILVLSDLDDEKRVLRALDLGVNDYLARPVDRNELVARVRTQIRHKR
                     YQDRLRQNVQSSLEMAIIDPLTKLHNRRYLTSQLSSMINDHMLRGGAISFIILDVDFF
                     KKVNDTYGHDAGDEVLIETAARMRRSIRGIDLLARFGGEEFVVVMPETDRFAGQHVAE
                     RLRRTIEATPFTIHKGARQIPITASFGLATMEGRVESAEGLAKRADEALYEAKRSGRN
                     RVILSNAA"
     gene            complement(2465..2632)
                     /gene="rpmG"
                     /locus_tag="PLQ11_11630"
     CDS             complement(2465..2632)
                     /gene="rpmG"
                     /locus_tag="PLQ11_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011179398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HRK25593.1"
                     /translation="MAKTATIKIKLLSTADTGYFYVTKKNARTATEKLSLKKYDPVAR
                     KHVEFKETKIK"
     gene            complement(2750..>3211)
                     /locus_tag="PLQ11_11635"
     CDS             complement(2750..>3211)
                     /locus_tag="PLQ11_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF839 domain-containing protein"
                     /protein_id="HRK25594.1"
                     /translation="WEIFLLCGDPSKPEIGANFHPATTKDGWFGMPDNLAIDSRNRLW
                     VGTDGNEPEKTGRGDGIWLVETEGPMRRRSTHFYRVPAGAECTGPFFTPNDETLFVSI
                     QHPGDDGPSYPAHGRFSSADDPSTRWPDFKPDMPPRPAVVAITRKGGGKVG"
BASE COUNT          632 a          978 c         1004 g          597 t
ORIGIN      
        1 aaaagcgatg agcgccgatt catcctcggc aagatagtcg agcacccgga tcaggaaggc
       61 cggatcggcc gccgcggcgc gcaaggttcc cgggccaagc ccggtcagcg ccaggaaccg
      121 gccgaggcgt tccgcatcgc cggcgatgaa acccagcgcg tcgatcgcca gcgcttcggg
      181 gtcaggatcg ggcagggcgc gcgccatcgg ggattccgca aactttaagg tctcgctgct
      241 atcaagaatg cgggagtgca acaggcttgg caagcggcgc gacctttcca acgaaggccg
      301 gcgccaggcg gcaggggacc aggataatgt caaaaacagt gctgatcgtc gaggataacg
      361 agttgaacat gaagctgttc aacgatctcc tgcaggcgca tggcatcaag acgctgctga
      421 ccagttccgg ggtcgaggcc atcgaactgg cgcgcaggca catgccggat ctgatcctga
      481 tggacatcca gcttcccgag gtttcgggaa tcgaggtgac caaatggttg aaggatgacg
      541 agcatctcaa gcgcattccg gtgatcgcca tcaccgcttt cgccatgaaa ggcgatgagg
      601 aacggatccg ccagagcggc tgcgaggggt atctttccaa gcccatctcg gtcggcaaat
      661 tcgtgcagac cgtgcaatcc tatctgggtg ccgcccgttg aaatgaacag accttccggg
      721 cgcgcgagcg cttcggatct gaaggggtat gaagtatgac tgcgcgtgtg ttgatcgtcg
      781 atgacctgtt tcccaacatc aaactgttgg aaacgcgcct tacggccgaa tatttcgatg
      841 tgctctcggc gatgaacgga atgcaggcga tcgagatctg cgaaagcggc gcctgcgaca
      901 tcgtcctgct cgatgtgatg atgccgggaa tggatggatt cgaggtttgc cgacggctga
      961 aatcttcacc aaccacggcg cacctgccga tcgtgatggt gaccgcgctc gaccagccgt
     1021 ccgaccggct caaggggctc gaagccggcg ccgatgattt cctgaccaag ccggtgaatg
     1081 atgtggcgct tctgacgcgc gtgcgctcgc tggccaggct caagacgctg accgatgaat
     1141 tgcgcgcccg tgcactcgcc actccgcagt tcggccagtc cgatcccctg gcagccgctg
     1201 ccgccgagga tggcctcaac ggacgtgtgc tgatcatcga cgatcgcgcc aattcctccg
     1261 agcgcatggc ctcaagcctg tcacaggtgc atcaggttga tgtcgaatcg gacccccagg
     1321 ccgcgctgat gcggttgtcc gagggcgaac atgacatggc catcatcagc cttggcctgg
     1381 ccaatttcga cgccctgcgc ctgtgcagcc agatgcgctc gctggagcgc acccgcaaca
     1441 cgccgatcct ggttttgtct gatctcgacg acgagaagcg ggtgctgcgt gcgctcgatc
     1501 tcggcgtcaa cgattatctg gcccgtccgg tcgaccgtaa cgaactggtc gcccgcgtac
     1561 gcacccagat ccggcacaag cgttatcagg acaggctgcg gcagaatgtg cagtcctcgc
     1621 tggaaatggc gatcatcgat ccgctgacca agctgcacaa ccgccgctat ctgacctcgc
     1681 agctgtcctc gatgatcaac gatcacatgc tgcgcggcgg ggcgatctcc ttcatcatcc
     1741 tcgatgtcga tttcttcaag aaggtcaacg acacctacgg ccacgatgcc ggcgacgagg
     1801 tgttgatcga aaccgcggcc cggatgcgcc gctccattcg cggcatcgat ctgctcgccc
     1861 gcttcggcgg cgaggaattc gtggtcgtga tgccggaaac cgaccgtttc gccggtcagc
     1921 atgtcgccga acggctgcgc cgcaccatcg aggcgacgcc gttcaccatc cacaagggtg
     1981 cccggcagat cccgatcacc gcctcgtttg gccttgccac catggaaggg cgggtggaat
     2041 ctgccgaggg cctcgccaag cgggccgatg aggcgctgta tgaagccaaa cgctcgggcc
     2101 gcaaccgggt gatcctgtcg aatgcagcct gaccaccggt gactttcgga tttggctgtt
     2161 aatccaagac cttaaggtaa acgggcaata aagccgccgg aaccgtctcc ggcggctttt
     2221 cctgtcgcgc agaccccgct agcgtcaaag gcaatcgggg aggcgtcaaa aaataccccg
     2281 tggaagaccc cgaaaggcat gagtatcggg tctgccgcat caacccggat tgcctgacgg
     2341 gcggccggtc ggacatggat tggagtggcc tcctcttcca tggctggccg gccactttat
     2401 gtgcgcgggc aacaaaaaag gcgccgcgaa cggcgcctca aacccccaag cgggcaaatc
     2461 caagttattt gatcttggtt tccttgaact cgacgtgctt gcgggcaacc ggatcatact
     2521 tcttcagcga aagcttctcg gtcgcggtgc gggcattctt cttggtcacg tagaaatagc
     2581 cggtatcggc ggtcgagagc agcttgatct tgatcgtggc ggtcttggcc attgtgtcat
     2641 cctccggcct cagccggccg tcaaaaaagg ttgggccgga ccaaaaggcc cggcgcatgc
     2701 ggggcttatg acctcaaccg gcagtcaaag tcaaggcatg atgggacatt cacccgacct
     2761 tgccgccgcc tttgcgggta atggccacga ccgcggggcg gggtggcatg tccggcttga
     2821 agtccggcca gcgcgtcgag ggatcatcgg cggatgagaa gcgtccgtga gctggataac
     2881 tgggcccgtc atcgcccgga tgctggatag agacaaagag cgtctcgtca ttgggggtga
     2941 agaacgggcc ggtgcattcg gcgccggccg gcacgcggta gaaatgggtc gagcgccggc
     3001 gcatcggccc ttcggtttcc accagccaga tgccatcgcc gcgcccggtc ttttccggct
     3061 cgttgccatc ggtccccacc cacagccggt tgcggctgtc gatggcgaga ttgtcgggca
     3121 tgccgaacca gccgtccttg gtcgtcgccg gatggaaatt ggcgccgatc tcgggcttcg
     3181 acggatcgcc gcacagcagg aagatttccc a
//
LOCUS       DAOMXG010000222         3197 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_16872_length_3197_cov_9.045831, whole genome shotgun sequence.
ACCESSION   DAOMXG010000222 DAOMXG010000000
VERSION     DAOMXG010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16872_length_3197_cov_9.045831"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            392..859
                     /locus_tag="PLQ11_11640"
     CDS             392..859
                     /locus_tag="PLQ11_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25595.1"
                     /translation="MSFLSPMARKGLAFAVLGTTLTVAMTDDAEARWRRHGHRGGAIA
                     AAAIGGLAAGALIASAGSAYASPSYGYGYGYTPVYSYGPSYSYAAHNVPATYSQGGGY
                     YAPAYATRTHYAPQRPRRVYRYASYREPICQITRKKVWLDSHTYTFRRVTRCY"
     gene            1002..1796
                     /locus_tag="PLQ11_11645"
     CDS             1002..1796
                     /locus_tag="PLQ11_11645"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009209890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HRK25596.1"
                     /translation="MSALPVLFVSHGAPNLVLHASPAHDFLQGLGKALPRPRAIVVIT
                     AHFMTRAPAVTADAAPETIHDFGGFEPELYQMTYKAPGAPELAADCLAALAEAGIDAQ
                     AVSNRGFDHGTWVPLMLMYPKADIPVVQLSVQPKAGPEHHLKLGMALRRLGEQGILVI
                     GSGALTHNLGEVFGGTYGFADPAPAWVQDFGEWMCEHVEAGDTAALLDYRARAPHAVR
                     NHPTEEHLLPLFSAIGAADGRPGRRLHSSAQYGVLMMDVFGFGMAA"
     gene            complement(1972..2823)
                     /locus_tag="PLQ11_11650"
     CDS             complement(1972..2823)
                     /locus_tag="PLQ11_11650"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRK25597.1"
                     /translation="MRRSAVLMVSAFLATGAQAADYLRGPLPEPVTRAAPTASYDWSG
                     TYFGAHYNFGSMSFNNYDLSGTAFNSGITNTTLRTNLATAHGNTLFGSANGNRSGYGV
                     FLGVNTQWDDVVLGLEGEYSRVGARANSRSTITGTFPAAAPAGTFDYNLTANARTTID
                     DYFALKARAGWAIDRTLLYATLGIAMANVTRQTTLTGTYEEYNVGPPRATISGVVPVD
                     SATKKSMARWGFATGLGAEYALTDAVLLRGEWQYLGFGSMTNLNNAGKNGYYSLNTVR
                     AGVAAKF"
BASE COUNT          583 a          950 c         1023 g          641 t
ORIGIN      
        1 ccgatcacgt tatccgcatt ttgatgattc catcaaaatg cggcgtgatc tagaggaagc
       61 gcggcggcgg tctggagcga ataaggcccg ccgccgcgac cttccaacca tccacggggg
      121 cacccgaaaa cgggtgtggg gcggaaggcc cggcaggtga tccctgccgg gcttttccgc
      181 attgcggatc gaagatcagt ccacacggtg gattgtattt cccctgtgca tcgcgatcag
      241 tccgatacac cggcaagttg gcccaaatca ttaatggcga ttaaccacgt ttcggcaaaa
      301 ctgttcattt ttgggacagg ataagctctt cactatgcga tgacgcggcg caggatccgc
      361 cggtttctgt aacggttgag gagggaagag catgtcattt ctgtcgccaa tggcccgcaa
      421 ggggctcgcg tttgccgttc tcggcactac gctgacggtt gccatgaccg atgatgcgga
      481 ggcccgctgg cgtcgtcacg gacatcgcgg tggcgcgatc gctgccgcgg caatcggtgg
      541 ccttgccgcc ggcgcgctga tcgccagcgc cggcagcgcc tatgcgtctc cgtcctatgg
      601 ctatggttat ggctatacgc cggtctacag ctatggcccc agctacagct atgcggccca
      661 caatgtgccg gcaacctatt cgcagggcgg cggctattat gccccggcct atgcgacgcg
      721 gacccattat gcgccgcagc ggccgcgccg cgtctatcgc tacgcttcct atcgcgagcc
      781 gatctgccag atcacccgca agaaggtgtg gctcgattcg catacctaca ctttccgcag
      841 ggtgacgcgc tgctattgag cgatgcatga tgtgcatcct tgcaatgcac atgctttgca
      901 ggtcctagaa aatggcgttc gcgcaaggac agctgcgcac ggacgccatt cgcatttcct
      961 gaatcccgct cttatgtctg ttgcaacaga gggattcagc catgtccgca ttacctgtcc
     1021 tgtttgtctc gcatggagcg cccaacctcg ttctccatgc ctctccggcg catgactttc
     1081 tgcaaggcct gggcaaggcg ctgccccggc cgcgcgccat tgtcgtgatc acagcccatt
     1141 tcatgacgcg cgctccggcg gtcactgccg atgccgctcc cgaaaccatc cacgatttcg
     1201 gcgggttcga gccagaactc tatcagatga cctacaaggc gcccggagcg ccggagcttg
     1261 ccgccgattg cctcgccgcg cttgccgagg ccggcatcga cgcgcaggcg gtcagcaatc
     1321 gcggctttga tcatggcacc tgggtgccct tgatgctgat gtatcccaag gcggacatcc
     1381 cggtggtgca gctgtcggtt cagccgaagg ctgggccgga gcatcatctg aagctcggca
     1441 tggccctgcg caggctgggc gaacagggca ttctggtgat cggttcaggg gcgctgaccc
     1501 ataatctcgg tgaggttttc ggcggaacct atggctttgc cgatccggcg ccggcatggg
     1561 tgcaggattt tggcgagtgg atgtgtgagc atgttgaggc gggggatacc gcagccttgc
     1621 tcgattaccg ggcgcgggcg ccgcatgcgg tgcgtaacca tcccaccgag gagcatctgc
     1681 tgccgctctt ttctgccatc ggagcggcag acggcaggcc cggccggcgg ctgcattcca
     1741 gcgcccaata cggcgtcttg atgatggatg tctttggctt cggcatggcc gcctgacgtg
     1801 atcgctcgca aatgttgaga gcaggctctt gccgcaaacg ccgacggccc gccgatcttg
     1861 gcgggccgct tttccatacg cgaggccagg tttggaaaga ggggcggcgg cgaccggttc
     1921 ggtcgccgga tcaggtcttg aaggctgtgg aatggcgcat cggtcgcggg gtcagaactt
     1981 cgccgcgacg ccggcacgca ccgtgttcag tgaataatag ccgttcttgc cggcgttatt
     2041 gagattggtc atcgagccga agccgagata ctgccactcg ccgcgcagca gcacggcatc
     2101 ggtcagggca tattcggcgc cgagtccggt ggcaaagccc cagcgggcca ttgacttctt
     2161 ggtggcactg tcaaccggga caacgccgga gatcgtggcg cgcggcggac cgacgttgta
     2221 ttcctcatag gtgccggtca gggtcgtctg ccgggtcaca ttggccatcg cgatacccag
     2281 cgttgcatac agcagcgtcc ggtcgatcgc ccagccggcg cgggccttca gcgcgaagta
     2341 atcgtcgatg gtggtgcgcg cgttggccgt cagattgtag tcgaatgtgc cggcaggggc
     2401 ggccgccgga aacgtgccgg tgatcgtcga acgggaattg gcccgggcgc caacgcggga
     2461 atattcgccc tcaaggccga ggaccacatc atcccactgc gtattgacgc caaggaatac
     2521 gccgtagccg gaccggttgc cgttggccga gccgaacagg gtgttgccgt gagcggttgc
     2581 cagattggtc cgcagcgtcg tgttggtgat gccgctgtta aaggcggtcc cgctcagatc
     2641 gtagttgttg aagctcatcg agccgaagtt gtaatgtgca ccgaaatagg taccgctcca
     2701 atcatagctt gccgtcggag cagcgcgcgt caccggctcg ggaagcggcc cgcgcagata
     2761 gtcggcggcc tgtgccccgg ttgcgagaaa agcggaaacc atgagaactg ctgaacggcg
     2821 catccggcca tctcctgcaa gcgccgcgac tcggcggctc caaacctgta acaacggtaa
     2881 aaaagtaacc ttaccgcccg gttaatcccg gaaaggaaaa agtgtgtctt tttcagtcgg
     2941 ttagcgaggg aggcggttta ccattcgcta acggtttggg tttgcctgga gcgttggggc
     3001 gcggccagag gcgcccgcca gcgatgttgt gggacgggcc aaggagcgct agagcatgtc
     3061 gcgatcaagt ggataccggc taatcgcaga agacatggcc agtttcgggc catggaccag
     3121 gaaaatgcga gaagcgtatc tggcgatccg gttcgagcat tttccgccgg atctggcccg
     3181 aaaatggcca tcccttc
//
LOCUS       DAOMXG010000223         3125 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_17424_length_3125_cov_8.631596, whole genome shotgun sequence.
ACCESSION   DAOMXG010000223 DAOMXG010000000
VERSION     DAOMXG010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17424_length_3125_cov_8.631596"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..691)
                     /locus_tag="PLQ11_11655"
     CDS             complement(80..691)
                     /locus_tag="PLQ11_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018428286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HRK25598.1"
                     /translation="MKLYDGGRVPNARRVRVFLAEKGVSVPTETVDLGTKQHKSRDFS
                     TLNPLQRVPVLVLDDGTILTESIAICRYFEEVHPEPPLFGEGARGRALVEMWQRRLEL
                     HLFLPVAYVFRHLHPAMADFEVPQVPAWGEANRARVHDYLAQLDRDLSGKDFVCENRF
                     SVADITGMIAIDFMKAGRIDMPPGLTNVTRWHAGLAARPSARA"
     gene            complement(959..1525)
                     /locus_tag="PLQ11_11660"
     CDS             complement(959..1525)
                     /locus_tag="PLQ11_11660"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HRK25599.1"
                     /translation="MRLSTLVSLIVLVLLFVWSTRQMDRPVPGDPLSNGRAEAVDGDS
                     LRMNGEEMRLLGIDAPEFSQTCKRGGQDWKCGREAAQHLRRLLARAPVTCIGHERDRY
                     NRLLVTCRSLGVDLGAAQVKAGMAIANGGYFVEEAEARNESRGLWAGEFDNPRDYRIL
                     HPSRQPAATPPQPQTPRPATVPTPPQRP"
     gene            1687..>3125
                     /locus_tag="PLQ11_11665"
     CDS             1687..>3125
                     /locus_tag="PLQ11_11665"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRK25600.1"
                     /translation="MPVVDVTADAIERKSGDEEVAAKRRAGLQRALKDTRERLTSSAG
                     LNRAFETELATTFASARIGASGPLMLLALLPAAASASWGDPVLIGLWIGAIWLTTSIY
                     HLLCHYFLKQAETMTNLSTWQVRFVIVEFSLALCWSGLTSVVGDPPSDSMALLLLFSL
                     MLFCAVSSIMSATMRPVVLANLAGSLTGVAKIFYHEGLASNVVLMLMVTGIMVFAYVL
                     SRRLFSSQLETLVYRAEKDDLIGELEQEKARSDEARKRAEDANLAKSRFLATMSHELR
                     TPLNAILGFSEVMQGELFGPHSVPQYKEYSNDIHSSGEHLLSLINEVLDLSRIEAGRY
                     SLTEEAVDLAGLVEDCRHMLDIRARKRELKVKDFYEPGLPRIWADERAIRQVVLNLLS
                     NAIKFTPQGSEITIKVGWTSRGGQYVSIRDNGAGIPEEEIPLVLETFGRGSQAMKSAE
                     PGSGLGLPIVKGLVELHGGTFVLRSKLREG"
BASE COUNT          573 a          987 c          990 g          575 t
ORIGIN      
        1 agccggtgcg gatagggata atgcgcgctt tcaaaggccg cctgcagcgc cgccacgcag
       61 gccaggagtg ccgcactcct catgcgcggg cgctcggccg ggcggcaagg ccggcatgcc
      121 agcgggtcac attggtcagt cccggcggca tgtcgatgcg gccggccttc atgaaatcaa
      181 tggcgatcat gccggtgatg tcggcgacgc tgaagcgatt ttcgcagaca aagtcctttc
      241 cagagagatc gcggtcgagc tgggccagat agtcgtgcac acgggcgcgg ttggcctccc
      301 cccaggccgg aacctgcggc acttcgaaat cggccatcgc cgggtgcaga tggcggaaca
      361 cataggccac cggcaggaac agatgcagtt cgaggcgccg ctgccacatt tcgaccaggg
      421 cgcgccctct ggccccctcg ccgaacaacg gcggctcggg atgcacttcc tcgaaatagc
      481 ggcagatggc gatcgattcg gtcagaatgg tgccatcgtc gagcaccaga accggcaccc
      541 gctgcaaggg attcaacgtc gagaaatcgc gcgacttgtg ctgctttgtg ccgagatcga
      601 cggtctccgt cggcaccgaa acgccttttt ccgcaagaaa cacgcgcacc cgccgcgcgt
      661 tcggaacccg accgccgtca tagagcttca tcaaacagtt ctcccgtgaa ccatccgtgc
      721 agcatggcat tttgccaggc aggtgaaaag cggcgcggcg gcggctgaca aaaaaacgca
      781 gccttgcggc cgcgtgaagt tggttcgctt gatatggcgc ccttgggcgt tggcaccatg
      841 ggcgcagtct tgctcccaac cgcgccctgc gatgtgcgct ggtgcacaat gcggcatctc
      901 tcgcatcgtt tttactgagc cgccgtccgc ccggcgcccg ttgcgggaca ttcaccgatc
      961 atggccgctg cggcggggtc ggaaccgtgg ccgggcgcgg ggtttgcggc tgcggcggcg
     1021 ttgcggccgg ctggcgggag ggatgaagga tgcggtaatc gcgcggattg tcgaattcgc
     1081 ccgcccagag accacgggac tcattgcgcg cctcggcttc ctcgacgaaa taacccccgt
     1141 tggcgatcgc catcccggcc ttgacctgag cggccccgag atcgacgccg aggctgcggc
     1201 aggtcaccag cagccggttg tagcggtcgc gctcatggcc gatgcaggtc accggcgcgc
     1261 gggccagcag gcggcgcagg tgctgggcag cctcgcgtcc gcatttccaa tcctggccgc
     1321 cgcgcttgca ggtctgcgag aattccggcg catcaatgcc cagaagccgc atctcttccc
     1381 cgttcatgcg caaggaatcg ccatccaccg cctcggcccg accgttggat agcggatcgc
     1441 ccggcacagg gcggtccatc tggcgcgtcg accagacaaa cagcaacacc agaacgatca
     1501 ggctgaccag tgtcgacagg cgcatgagga tcggaccaga ataccgcgat cgaagagccg
     1561 ggcgcggagt taagcagaaa agtgtcgaac cagacagatt atccagcctt gtgaatcagc
     1621 ttttaacgtt tttatcggca gagtgcagtg ttgttcctgg ttttcactgc gtgttcccat
     1681 ccgtcaatgc ccgttgttga tgtcacagcc gacgctatcg agcgcaaaag cggcgacgaa
     1741 gaggtcgccg ccaagcgccg cgccggcttg cagcgcgcgc tcaaggacac gcgtgaacgg
     1801 ctgacatcgt cggccggcct caaccgggcc ttcgagaccg aactcgccac cactttcgcc
     1861 agcgcccgca tcggcgcctc ggggccgctg atgctgctgg cgctgctgcc ggcggcggcg
     1921 tcagcctcgt ggggtgatcc ggtgctgatc ggtttgtgga tcggcgccat ctggctgacc
     1981 acctcgatct atcatctgct ctgccactac ttcctcaagc aggctgagac catgaccaac
     2041 ctgtcgacgt ggcaggtgcg gtttgtcatc gtcgaattct cgctggcctt gtgctggagc
     2101 gggctgacct cggttgtcgg cgatccgcct tcagacagca tggcattgct gctgctgttc
     2161 tcgctgatgc tgttctgtgc tgtcagctcg atcatgtcgg cgacgatgcg cccggtggtg
     2221 ctggccaatc ttgccggatc gctgaccgga gtggcgaaga tcttctatca tgaaggtctg
     2281 gcctccaatg tcgtgctgat gctgatggtc accggcatca tggtgtttgc ctatgtgctg
     2341 tcgcggcggc tgttcagttc gcagctcgaa accctggtct atcgcgccga gaaggatgat
     2401 ctgatcgggg aactggagca ggaaaaggcc cgttcggacg aggcccgcaa gcgcgccgaa
     2461 gatgccaatc tggccaagtc ccggttcctt gccacgatga gccacgaatt gcgcacaccg
     2521 ctgaacgcca ttctcggctt ctccgaggtc atgcagggcg agctgttcgg accgcattcg
     2581 gtgccgcaat acaaggaata ttccaacgat atccattcga gcggagagca cctgctgtcg
     2641 ctgatcaacg aagtgctcga cctgtcgcgg atcgaggccg gccgctactc gctcaccgaa
     2701 gaggcggtag atctcgccgg tctggtcgag gattgccgcc acatgctcga catccgcgcc
     2761 cgcaagcgtg aactgaaggt caaggatttc tacgaacccg gactgccccg catctgggcc
     2821 gacgagcgcg ccatccgcca ggtggtgctc aaccttttgt cgaacgccat caagttcacg
     2881 ccccagggca gcgaaatcac cataaaggtg ggctggacct cgcgcggggg gcaatatgtc
     2941 tccatccgcg acaatggcgc cggcattccc gaggaagaaa ttccactggt gctggaaacc
     3001 ttcgggcgcg gctcgcaggc gatgaaatcc gcagaaccgg gcagcggcct cggcctgccg
     3061 atcgtcaagg ggctggtcga actgcacggc ggaaccttcg tgctgcgctc caagctgcgc
     3121 gaagg
//
LOCUS       DAOMXG010000224         3082 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_17803_length_3082_cov_9.519987, whole genome shotgun sequence.
ACCESSION   DAOMXG010000224 DAOMXG010000000
VERSION     DAOMXG010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17803_length_3082_cov_9.519987"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..865)
                     /locus_tag="PLQ11_11670"
     CDS             complement(176..865)
                     /locus_tag="PLQ11_11670"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRK25601.1"
                     /translation="MLIIYDCDGTLIDSEGIACSVCAEALTAIGVPYTTAMFAERYAG
                     RPARETWAHVGATYGVTFPPGFNEAINTEIHARLDAGVQAVEGVRAAILAIDGARCVA
                     SSTGTRQLNRNLATAGLADLFGEHVFSVHQVKRGKPAPDVFLFAASQMGADPDQCLVI
                     EDTVAGVTAARRAGMRALGFTGANHGGPGLDQRLIAAGASAIIVHMRDLPQAVSAHRA
                     GPMGTQAKRPA"
     gene            958..1851
                     /locus_tag="PLQ11_11675"
     CDS             958..1851
                     /locus_tag="PLQ11_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HRK25602.1"
                     /translation="MVKKSLDKIVRKLRVDDGDGFKLKDYDTKNPLGKALDEKMEAID
                     ILEAGIKDLKEIQEVLFAQDEWSVLLIFQAMDAAGKDSTIKHVMSGINPQGCHVAAFK
                     RPSEEELDHDYMWRCLKQLPERGRIGIFNRSYYEEVLVVRVHPEILDKQHLPAELKKD
                     PDIWDKRLKDIRHFENYLARNGTRIIKFFLHVSKEEQKKRLLARLDEPEKNWKFELGD
                     LKERALWDDYMKAYQEAIRGTATDAAPWYVIPADNKWFMRAAVVRAILHELKGLDLAY
                     PEVTKAQLQDFADARKRLMAE"
     gene            1859..2818
                     /locus_tag="PLQ11_11680"
     CDS             1859..2818
                     /locus_tag="PLQ11_11680"
                     /inference="COORDINATES: protein motif:HMM:NF013885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25603.1"
                     /translation="MIAGALAALGRRGAEGFALSILLGLAMPQFAAEARPLLPLTIFG
                     FMTVVFLRADFSVIGGLIRRPVRLLAAMGWQLLAPVVLVLLTIRLIGSSTIDPGLLLG
                     LAVLGAAPPIMSSPAVAILYGFEPSLVIASVLGLTLLSPLTAPLLVDVLAGQAVPLDQ
                     AVLMQRLLIFVGGGIAAAMVLRRWLGTDRVRSLKAELDGFGVVMYFVFAVAAMDGVTA
                     AAMSRPGLVAGFVGLAFGLALAGLSLAILALRGLDEPDRFMVGYGTAQRNMGLLIAAL
                     GAGVPSTTFLFFALAQFPIYLMPWILKPVAARIANRRAVGDGG"
     gene            2835..>3082
                     /locus_tag="PLQ11_11685"
     CDS             2835..>3082
                     /locus_tag="PLQ11_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014889576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRK25604.1"
                     /translation="MRALIVALGLALVIPAADAQEQRRRDHIEQCFRCHGDDGIAKDR
                     DVPHLAGQQEAYLYAQMRAFSSGKRPHKEMKVMSREMN"
BASE COUNT          529 a          927 c         1043 g          583 t
ORIGIN      
        1 gcctatcggg ccggcaactg ggaggacgcc gaggccgcac tggcggtggt tgccccgctg
       61 gccgaccggt tcggattggg cgggttcgtg acggtgatgc ggcggcggat cgcccggctc
      121 aggctggccc catccgggga ctggaccggc atttccgagc cggaggagcg atagatcacg
      181 ccgggcgctt ggcttgcgtg cccatgggcc cggcgcggtg cgccgacacc gcctgcggca
      241 ggtcccgcat atggactatg atcgccgatg cgccggcggc gatcagccgc tggtcgagcc
      301 ccggtccgcc atggttggcg ccggtgaaac caagggcgcg cattcccgcc cgccgtgcgg
      361 cggtcacacc tgccacggta tcctcgatca ccagacattg atcgggatcg gcgcccatct
      421 gcgaggccgc aaacagaaag acatccggcg ccggcttgcc gcgcttgacc tgatgaacag
      481 aaaacacatg ttcgccgaac aggtcggcaa ggccggccgt ggccagattg cggttgagct
      541 ggcgcgtgcc ggtggaagag gcgacgcaac gcgccccgtc aatggccagg atcgccgcgc
      601 gcacgccttc gaccgcctga acgccggcat cgaggcgggc atggatctcg gtgttgatcg
      661 cttcgttgaa cccgggggga aaggtgacgc cataggtggc gccgacatgc gcccaggttt
      721 cgcgggccgg ccgtccggca tagcgctcgg cgaacatcgc cgtggtatag ggcacaccga
      781 tggccgtcag cgcctcggca cacacggaac aggcaatgcc ctcgctgtcg atcagcgtgc
      841 catcgcaatc gtagatgatc agcattcgca ccttcttcga tttgcgctct gacaatgtca
      901 tttgtcaggc atggcgcaat ttgtcatgct cagcctgtca tttctggaag gcgtgccatg
      961 gtcaagaaga gcctcgacaa gattgttcgc aaattgcgcg tcgacgacgg cgacggtttc
     1021 aagctgaagg attacgacac caagaatccg ctcggcaagg cgcttgatga aaaaatggag
     1081 gcaatcgaca ttctcgaggc cggcatcaag gatctgaagg agatccagga ggtgctgttc
     1141 gcccaggacg aatggtcggt gctgctgatc ttccaggcga tggatgcggc tggcaaggat
     1201 tccaccatca agcatgtgat gtcgggcata aatccgcagg gatgtcatgt cgccgcgttc
     1261 aagcgcccgt ccgaggaaga actcgatcac gattacatgt ggcgctgcct gaagcagctg
     1321 ccggagcgcg gccgcatcgg catcttcaac cgttcctatt acgaggaagt gctggtggtg
     1381 cgcgtccacc ccgagattct cgacaagcag catctgccgg ccgaactgaa aaaggatccg
     1441 gacatctggg acaagcggct gaaggatatc cgccacttcg agaactatct tgcgcgcaac
     1501 ggcacacgca tcatcaagtt cttcctgcat gtgtcgaagg aagaacagaa gaagcggctt
     1561 ctcgcccgac tcgacgaacc tgagaagaac tggaagttcg aactcggcga cctcaaggaa
     1621 cgggcgctgt gggacgatta catgaaggcc tatcaggagg cgatccgcgg cacggcgacc
     1681 gacgccgccc cgtggtatgt cattccggcc gacaacaagt ggttcatgcg cgccgccgtc
     1741 gtgcgcgcca tcctccatga gctcaagggg ctcgatctcg cctatcccga ggtcaccaag
     1801 gcacagctcc aggatttcgc cgacgcacgc aaacggctga tggctgaatg acatgggaat
     1861 gatcgcgggg gctcttgccg cgcttggccg gcggggagcg gaggggtttg cgctgtcgat
     1921 cctgctgggg ctggcgatgc cgcagttcgc agccgaggcg cgcccgctgc tgccgctgac
     1981 catcttcggg ttcatgacgg tggtgttcct gcgcgccgat ttttcggtca tcggcggact
     2041 gatccgccgg ccggtgcggc tgttggcggc aatgggctgg cagcttctgg cgccggtcgt
     2101 tctggtgttg ctgacgatcc ggctgatcgg ctcatcgacc attgatcccg gcctgctgct
     2161 cggtcttgcc gtgctcggcg ccgcgccgcc gatcatgtcc tcgccggcgg tggcgatcct
     2221 ctacgggttc gagccgtcgc tggtgatcgc ctcggtgctg gggctgacgt tgctcagccc
     2281 gctgaccgcg ccgctgctgg tcgatgtcct ggccggacag gccgtgccgc tggatcaggc
     2341 cgtcctgatg caacggctgc tgattttcgt tggcggcggc atcgcggcgg ccatggtcct
     2401 gcgccgctgg ctgggaacgg acagggtccg ctcgctgaag gctgaactcg atggcttcgg
     2461 tgtggtgatg tatttcgtct ttgccgtggc ggcgatggat ggcgtgactg ccgccgcgat
     2521 gagccggccg gggctcgttg cggggtttgt cggtctggcg ttcggtctgg cgctggccgg
     2581 actcagtctg gcgatcctgg ccctgcgcgg ccttgatgag ccggaccgct tcatggtcgg
     2641 ctacggaacc gcccagcgca acatgggtct gctcatcgcc gcgctgggag ccggcgttcc
     2701 gtcgacgaca ttcctgtttt ttgcattggc gcagtttccg atctatctca tgccgtggat
     2761 tctgaagccg gtggccgccc gaatcgcaaa ccgcagggca gtcggggatg gcggttgacc
     2821 ggaaggagat ggcgatgcgt gccttgattg tggctctcgg actggcgctg gttatcccgg
     2881 cggcggacgc ccaggaacag cggcggcggg atcatatcga acaatgcttc cgctgccatg
     2941 gtgacgatgg catcgccaag gatcgcgatg tgccgcatct ggccggccag caggaagcct
     3001 atctctatgc gcagatgcgg gccttttcgt ccggcaagcg gccgcacaag gagatgaagg
     3061 tgatgagccg cgaaatgaac ga
//
LOCUS       DAOMXG010000225         2969 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_18812_length_2969_cov_11.563487, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000225 DAOMXG010000000
VERSION     DAOMXG010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18812_length_2969_cov_11.563487"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="PLQ11_11690"
     CDS             complement(<1..675)
                     /locus_tag="PLQ11_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25605.1"
                     /translation="MLGAVIAVLPIMAAGAVVIGTSRDLNEVAFAMLAVAGMLSLAGI
                     GLLWTLRNAQGQLEARSTETRHLVLALTRSMRHLAQRVGGLEASTQAVARLPDWMPGA
                     ANIPAPAPPVLIERRLLTVPQRRLAGIEIVAEWGEPGNDAISAGYRQMGAEGDVIERI
                     EAAVALAGRPLLPENAFILVSIDQPLAGMSLAIEAILALTVARPPVAARLVIGISQRA
                     LRLGGNS"
     gene            830..1270
                     /locus_tag="PLQ11_11695"
     CDS             830..1270
                     /locus_tag="PLQ11_11695"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRK25606.1"
                     /translation="MPRFFNPKTTARPSSKFSHGVVHSARARRLVISGQVGVLADGTI
                     LPDLEGQMQAAWDNLIATLEEAGMGITDLIKITAFVTVPGAVNVFRKVRDQRLMGHCP
                     ASTYLEVSGLARPDFMFEIEGEAVVEEADMIADQWTETVARGHA"
     gene            1292..1492
                     /locus_tag="PLQ11_11700"
     CDS             1292..1492
                     /locus_tag="PLQ11_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018261969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HRK25607.1"
                     /translation="MGELVLNVGGMGCSGCVDAVTRAVEKAAPGAAVTVDLASGRVTV
                     RTDASRETIAGAIARAGYDIKD"
     gene            1589..2900
                     /locus_tag="PLQ11_11705"
                     /pseudo
     CDS             1589..2900
                     /locus_tag="PLQ11_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440083.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
BASE COUNT          550 a          928 c          941 g          550 t
ORIGIN      
        1 gctgttgcca ccgagccgta acgcgcgctg gctgatcccg atcaccaacc gggcggcgac
       61 cggcggccgc gcaaccgtca gggcaagaat agcctcgatt gccagcgaca ttccggcaag
      121 tggctgatcg atcgagacca ggataaaggc attttccggc aacaacggtc gcccggccag
      181 agccaccgcc gcttcgatcc gttcgatcac atcgccttcg gcacccatct gccggtatcc
      241 ggccgaaatg gcatcgttgc ccggctctcc ccattccgcg acgatctcga ttccggccag
      301 ccgccgctgc ggaacggtca gcaggcgtct ttcgatcaga accggtggcg ctggcgcagg
      361 gatattggct gcccctggca tccagtcggg caggcgcgca acagcctggg ttgaggcctc
      421 aagcccgccg acccgctggg ccagatgcct catcgatcgg gtcagagcga gcaccagatg
      481 ccgcgtttcg gtgctgcggg cttccagttg cccctgggca ttccgcagcg tccacagcag
      541 cccgatcccg gccagcgaca gcatgccggc aaccgccagc atggcaaagg ccacctcgtt
      601 caggtccctt gatgtgccaa tcacgaccgc gcccgcagcc atgatcggca gcacggcgat
      661 gaccgctcca agcatccttg caagaacccg ccctgccggc acacccgccc ccgtttgcca
      721 tgaccggcgc cggatttggc gaatctggtc agtggacgta tgagtggaga gagtcgacgc
      781 tacaacctta agattgagtt aataaacaat acaactagaa agggaaatcg tgccgcgctt
      841 tttcaatccc aagacgaccg ctcggcccag ctcgaaattc tcgcatggcg tggtccattc
      901 ggcccgggcg cgtcgtctgg tgatttccgg gcaggtgggt gtgcttgccg atggcaccat
      961 cctgcccgat ctggagggtc agatgcaggc ggcctgggac aatctgatcg ccacgctgga
     1021 agaggcgggc atgggcatca ccgacctgat caagatcacg gccttcgtca ccgtgcctgg
     1081 ggcggttaat gtgttccgca aggtgcgcga ccagcgtctg atgggccatt gtccggcctc
     1141 gacctatctg gaggtcagcg gtctggcccg acccgatttc atgttcgaga tcgaaggcga
     1201 ggcggtggtc gaagaagccg acatgattgc cgatcaatgg accgaaacgg ttgcgcgtgg
     1261 ccacgcataa tcgagaactc cggagcggat gatgggcgaa ctggttttga atgtgggcgg
     1321 catgggctgc tcgggctgtg tggatgcagt cacgcgggcg gtcgaaaagg cggcgcccgg
     1381 cgcggcggtg acggtcgatc ttgcctcggg cagggtgacg gtccgcaccg atgccagccg
     1441 cgagacgatc gccggcgcca tcgccagggc tggctacgac atcaaggatt gatgccggca
     1501 atcgattggc gtgatgcatt cgatcacgcc agcgcatttg acctcggcca gcgtgctggt
     1561 agcatggggc ctctgatgga gacccgacat ggccgatgct gcccgcccgc tcaatccgcg
     1621 cgttccgaat gacctttcgg cgttcttcat gccgtttacg gccaatcgag cgttcaagaa
     1681 gcgaccgcgg ctgatcgcaa gtgccaaggg catgtattat tacacaccgg acgggcgcga
     1741 ggtgatggac gcggcggcgg gtctttggtg caccaatgca gggcataacc atccgcgcat
     1801 cgtcgcggcg atccaggcgc aggccaacga tctggatttc gcgccctcgt tccagttttc
     1861 ccacccgctg gcgttcgagg ctgcctcgcg cgttgcggcg ctggcgccgg gcaatctcga
     1921 ttcggtgttt ttctgcaatt ccgggtcgga ggcggtcgat acagcgctga aaatcgccgt
     1981 tgcctattgg cggtcgtgaa cgtggctatc atggcatcgg catgggcggc atttcggtcg
     2041 gcggcatcgt tgccaaccgc aagcagttcc attcgatgat gatcccgaat gtcgatcatc
     2101 tgccgcatac ctacaaccgc gccgagcagg ctttttccaa gggctcgccg gcatggggcg
     2161 cgcatcttgc cgatgagctg gatcgactgg tggtgctgca tgatgcctcg accatcgccg
     2221 ctgtcattgt cgagccgatg gcgggatcga ccggcgtgct gccgccgccg caagggtatc
     2281 tcaaacgcct gcgcgagatc tgcaaccgcc acggcatcct gctgattttc gacgaggtca
     2341 ttaccggctt tggccgcacc ggcaatgcct ttgcctcgat caccgagggc gtgacgccgg
     2401 atctgttcac cttcgccaag ggcgtcacct ccggcacggt gccgatgggc ggcgtcgtct
     2461 gccgcaagga tatttacgag accctgatgc agggccccga gcatgtcgtg gaattctgcc
     2521 acggctacac ctattcggcc catccgctgg cctgcgcggc agcgatcgcg gcgatggatg
     2581 tctatcgcga cgagaaactg ttcgagaatg ccaagaagct cgagccggcc tggcaggagg
     2641 cgatctacag tctcaagggc ctgccgaatg tgctcgacat ccggcaatac ggcatcaccg
     2701 ccggtatcga tctggcctcc aggcccgatg cgttcggcaa gcgcgcctat gaggcgatgg
     2761 aagccggctt ccatgatctc ggcattgttg tgcgcacaac gggcgaaacg atcgcagtga
     2821 ccccgccgct gaccttgaca gaaaaacaca tcgacgaatg tgcgaccaag ctcgccaagg
     2881 tgatcaaggc ggtggcgtaa acgctgatta atccccctcg ccccgctgcg cggggagagg
     2941 gtgacccaag cgaagcggag ggtcgggag
//
LOCUS       DAOMXG010000226         2969 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_18814_length_2969_cov_9.784832, whole genome shotgun sequence.
ACCESSION   DAOMXG010000226 DAOMXG010000000
VERSION     DAOMXG010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18814_length_2969_cov_9.784832"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="PLQ11_11710"
     CDS             <1..367
                     /locus_tag="PLQ11_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Synthesizes thioester adducts of fatty acids
                     enzymatically to the phosphopantetheine group of acyl
                     carrier protein; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25608.1"
                     /translation="GGGGVAAVLADPQAGPIAASLVGLAMAGGVFIVPSFAAAQNWAA
                     KERRARVVAAINVLNAAFMVAGTLLVGALQARGLSVAGAFGLIAAVCLVSALQIWRTF
                     PGNPTLELAALRARFRLRD"
     gene            474..1073
                     /locus_tag="PLQ11_11715"
     CDS             474..1073
                     /locus_tag="PLQ11_11715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRK25609.1"
                     /translation="MFQVRIHGRGGQGVVTGAELLSVAAFLEGQHAQAFPSFGSERTG
                     APVVAFCRISDREIRLREPILEPDCLIIQDPTLFKVVDVFGGLKSSGFLIVNTNKSFA
                     DLHIEQVAVRLPKGHAVIVPATEIAQRHVGRPVPNAALLGAFAALTELVHFDSVKAAI
                     EEAFPGKIGAANIAAAEEAHAFVVAQEAAAGVTDHAAHV"
     gene            1083..2354
                     /locus_tag="PLQ11_11720"
     CDS             1083..2354
                     /locus_tag="PLQ11_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRK25610.1"
                     /translation="MLKQIEGSRAMAEAIALCRPEVICAYPITPQTHIVEGLGELVKD
                     GHLTGCEYINVESEFAALSVAIGSSAAGARSYTATASQGLLFMAEAVYNASGLGLPIV
                     MTLGNRAIGAPINIWNDHSDAMSMRDAGWLQLFAETNQEAVDLHIQAFRLAEELSMPV
                     MVCVDGFILTHAVERVDIPDQKEVDAYLPPFEPVQVLDTADPASIGAMVGPEAFTEVK
                     FLAHYKQTQALRLIPRLSGEFRSRFGRDSGGLIRSYRTEDADTIVVAMGSVCGTIKDT
                     IDEMRADGFNIGLVTIISYRPFPSAALRNALAGAKDLIVLEKSIAVGMGGPLATDVDE
                     ALRNLPHAPILHSAVAGLGGRPILKSSLHRLFRQAIVQPWEGTHFLDLNERVIGREIH
                     RTGKRRRSGPTAENILRQLADQDREANKAGR"
     gene            2357..>2969
                     /locus_tag="PLQ11_11725"
     CDS             2357..>2969
                     /locus_tag="PLQ11_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRK25611.1"
                     /translation="MSGEPHDTLAPGEAAQKLRYYQKGTFTVGNRLVDAEARSVQASG
                     NRSNALTCGHRACQGCGEALGARYALDAAMRATNSQLIAVNATGCLEVFTTPYPETSW
                     QLPWLHSLFGNAAAVATGVAAALRVKGKSNVRVIAQGGDGGTTDIGFQCLSGMFERND
                     DVLYICYDNEGYMNTGVQRSSATPPAARTATTPAVGGEPGNVFD"
BASE COUNT          488 a          902 c         1046 g          533 t
ORIGIN      
        1 gggcggtggc ggggttgcgg cggtgcttgc cgatccgcag gccgggccga tcgcggccag
       61 ccttgtcgga ctggccatgg ccggcggagt attcatcgtt ccctcctttg ctgcggcgca
      121 gaactgggca gcgaaggagc ggcgggctcg ggtggtggcg gcaatcaatg tgctcaacgc
      181 tgccttcatg gtggcaggaa cgctgttggt cggtgccttg caggcgcgcg gcctgtcggt
      241 tgccggtgcc ttcgggctga tcgctgcggt ctgtctggtc tcggccctgc agatctggcg
      301 gacgtttccc ggcaatccga cgcttgaatt ggcagccctg cgcgcccgct tcaggttacg
      361 ggattgacag cggcaaaggc tcctgtcctg ttgatgttgg tcaaagcgct gcccggcaga
      421 acatgcttgg cagtggcgca taaaaatggc ggcccgttcg gggaggggat ggcatgtttc
      481 aggtgcgcat acacgggcgc ggcgggcaag gcgtcgtgac cggcgccgag cttctgtcgg
      541 ttgccgcgtt tctcgaaggc cagcatgcgc aggcgtttcc cagtttcgga tcggagcgga
      601 cgggcgcacc ggtggtggcg ttctgccgta tctcggacag ggaaatccgg ctgcgtgagc
      661 cgattctgga acccgattgc ctgatcatcc aggatccgac gctgttcaag gtcgtcgatg
      721 tgttcggcgg gctgaaatcc agcgggttcc tgatcgtcaa taccaacaag agctttgccg
      781 atctgcatat cgagcaggtg gcagtgcggc tgccgaaggg ccatgcggtg atcgtgccgg
      841 cgacggagat cgcccagcgg catgtcgggc gcccggtgcc gaatgcggcg ctgctcgggg
      901 cctttgcggc gctgaccgag ctggtgcatt tcgacagcgt gaaggcggcg atcgaggaag
      961 cgtttccggg caagatcggc gcggccaata tcgccgcagc agaggaggct cacgcctttg
     1021 tcgtggcgca ggaagcggcg gcaggcgtga ccgatcacgc cgcgcacgtc tgaggagggg
     1081 acatgctgaa gcagattgaa ggctcgcgcg ccatggccga agccatcgcg ctgtgccggc
     1141 cagaggtgat ctgtgcctat ccgatcacgc cgcagacgca tatcgtcgag ggcctcggcg
     1201 aactggtcaa ggacggccat ctcaccggct gcgaatacat caacgtcgag agcgaattcg
     1261 ccgccctgtc ggtggccatc ggctcgtcgg cggcgggagc gcggtcttat accgccaccg
     1321 cctcgcaggg gctgctgttc atggcagaag cggtctacaa cgcctccggt ctcggcctgc
     1381 cgatcgtgat gacgctcggc aaccgggcga tcggtgcccc gatcaacatc tggaacgatc
     1441 attccgatgc gatgtcgatg cgcgatgcgg gctggctgca gttgttcgcc gagaccaatc
     1501 aggaggcggt cgatctgcat atccaggcgt tccggctggc tgaggaactg tcgatgccgg
     1561 tgatggtctg cgttgacggc ttcatcctca cccatgcggt ggagcgggtc gatattcccg
     1621 accagaagga ggtcgatgcc tatctgccgc cgttcgagcc ggtgcaggtg ctcgataccg
     1681 ccgatccggc gtcgatcggt gcgatggtcg ggccggaagc cttcaccgag gtgaagtttc
     1741 tcgctcatta caagcagacg caggcgctgc ggctgatccc gcggctgtca ggcgagttcc
     1801 gcagccgctt cggccgtgat tccggcggac tgatccgcag ctaccgcacc gaggatgccg
     1861 ataccatcgt cgttgccatg ggttcggtct gcggcacgat caaggatacc atcgatgaga
     1921 tgcgggccga cgggttcaac atcgggctgg tgacgatcat ctcctaccgc ccgttcccct
     1981 cggcggcgct gcgcaatgcg ctggccggcg ccaaggacct gatcgtgctg gaaaagagca
     2041 tcgcggtcgg catgggcgga ccgctcgcca ccgatgttga cgaggctttg cgcaacctgc
     2101 cgcatgcgcc gatcctgcat tcggcggtgg cgggcctggg cgggcgaccg atcctcaaat
     2161 cgtcgctgca ccggctgttc cggcaggcca tcgtgcagcc ctgggagggc acgcattttc
     2221 tcgatctcaa cgagcgggtg atcggccggg aaatccaccg caccggcaag cgccgccgct
     2281 cggggccgac ggcggagaac attctccggc aactggccga tcaggatcgc gaagccaaca
     2341 aggccgggag gtaacgatga gcggcgaacc ccacgatacg ctggccccgg gcgaggccgc
     2401 gcagaagctg cgctattacc agaagggcac attcaccgtc ggcaaccggc tggtcgatgc
     2461 cgaggcccgg tcggtgcagg cctccggcaa ccggtcgaat gcgctgacct gcggccatcg
     2521 tgcctgccag ggctgcggcg aggcacttgg cgcgcgttat gcgctcgatg cggcgatgcg
     2581 ggcgaccaac agccagctga tcgcggtgaa cgcgaccggc tgtcttgagg tgttcaccac
     2641 gccctatccg gaaacctcat ggcaattgcc gtggctgcat tcgctgttcg gcaatgcggc
     2701 ggcggtggcg accggggtcg cggcggcgct gcgcgtcaag ggcaagtcga atgttagggt
     2761 gatcgcgcag ggcggcgatg gcgggaccac cgacatcggc ttccagtgcc tgtccgggat
     2821 gttcgagcgc aacgatgacg tgctctacat ctgctacgac aacgaaggct atatgaatac
     2881 cggcgtgcag cgatcctcgg cgacaccgcc ggcggcgcgg acggccacca ccccggcggt
     2941 cgggggcgag ccgggcaatg tgttcgata
//
LOCUS       DAOMXG010000227         2860 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_19867_length_2860_cov_9.014617, whole genome shotgun sequence.
ACCESSION   DAOMXG010000227 DAOMXG010000000
VERSION     DAOMXG010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19867_length_2860_cov_9.014617"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1651)
                     /locus_tag="PLQ11_11730"
     CDS             complement(<1..1651)
                     /locus_tag="PLQ11_11730"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HRK25612.1"
                     /translation="MHTVIYSGQGTPVETDRREAHQPPFRQSGTLVAIDGTGATIATR
                     VPALLSVDAPYWAVGTLITVYGQRSRVICFVKHMGLRDGAWQEQGDNPITADVELLGE
                     IVDLPNGRPEFRRGISFYPPIGSPAHRIRREDLMAIYDLGGRKGSTIGTLSQDADIAA
                     SVDVEGMLKKHFAVVGTTGVGKSCSMSILLRTAIETRPGLRVVIADPHNEYTNAFRDV
                     ARTVDSASFELPFWLFRFEEIVEVIYRGAPAPEEEIEFLRDAIVQAKEMFNARAQAQP
                     NSILKKTFRSDGGTVTADSAQPYRMTDVIALIDEATGRLEVKFQRSSLRSLRARIESL
                     SNDPSYRYMFGKAAIDGMTEPVIRALFRLTERNQPITILQLAGIPADVVNASVSVLAR
                     MAFDLTMVSSGRYEILMVCEEAHRYVPADPALGFAPTRRALARIAKEGRKYGCYLAVV
                     TQRPSELDPTILSQCSTIFAMRLANAQDQAIMRSAMPDAATGVLEFLSALANREAIGF
                     GEGFSTPMRFLFRMTDTGSLPKMMDDGSSKVGVINSQAGNQD"
     gene            complement(1735..2445)
                     /gene="fliP"
                     /locus_tag="PLQ11_11735"
     CDS             complement(1735..2445)
                     /gene="fliP"
                     /locus_tag="PLQ11_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003604002.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HRK25613.1"
                     /translation="MALLPLVAGLATPAAAQDIAVNLGGAGLSERALHLMALLTVLAL
                     APSILVMATSFTRIVVALSLLRSALGTQSAPPNMVLIALSLFLTAFVMAPTFEAAYNG
                     GIKPLVAGEIRESEAFERTVRPFHAFMLRNAREKDVELFVSLSRKPRPDTPEALPLTT
                     LVPAFMISELRRAFEIGFLLFLPFLVIDLIVASILISMGMMMIPPVMVALPFKLVFFV
                     VVDGWRLVAGSLVQSFSA"
     gene            complement(2487..>2860)
                     /locus_tag="PLQ11_11740"
     CDS             complement(2487..>2860)
                     /locus_tag="PLQ11_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25614.1"
                     /translation="PAAADPRLSDMARRLQSVLNRPLSPLRPAQPVDAKAAAEAETKP
                     IPLPSAAQRAKDPEPVAAAAEEPPAKPAKPAKSFEKPAPAPVTQGKDLPPAPIAPDSQ
                     TIDSLEEEMARLLGRSGPGKT"
BASE COUNT          544 a          884 c          944 g          488 t
ORIGIN      
        1 aatcctggtt tccggcttgt gaattgatca cgccgacctt ggacgagccg tcgtccatca
       61 tcttcggcag gctgccggta tcagtcatgc ggaacaggaa ccgcatcggc gtcgaaaatc
      121 cttcgccgaa tccgattgcc tcgcgattgg cgagcgcgga gaggaattcc agcactccgg
      181 tcgcggcatc tggcatcgcc gagcgcatga tggcctggtc ctgcgcgttg gcgaggcgca
      241 tggcgaagat cgtcgagcat tgcgacagga tggtcgggtc cagttcactc ggccgctgcg
      301 tgaccaccgc gagatagcag ccgtatttgc gtccttcctt ggcgatgcgg gccagcgccc
      361 gccgcgtcgg cgcaaaaccc agcgccggat cggcgggcac atagcgatgg gcttcttcgc
      421 agaccatcag gatctcataa cgtccgctgc ttaccatggt caggtcaaac gccatgcggg
      481 ccagcaccga gaccgaggca ttgacgacat cggccggaat gccggcgagc tgcagaatgg
      541 tgatcggctg gttgcgctcg gtcaggcgga acagggcgcg gatgaccggt tcggtcatgc
      601 cgtcgatcgc agccttgccg aacatgtagc gatagctcgg atcgttcgac aggctctcga
      661 tacgcgcccg cagcgaccgc agcgaggagc gctggaactt cacttccagc cggccggtcg
      721 cctcgtcgat cagcgcgatg acatcggtca tgcgataggg ctgggccgaa tccgccgtga
      781 cggttccgcc atcggagcgg aaggtctttt tcaggatcga attcggctgg gcctgggcgc
      841 gggcgttgaa catctccttg gcctggacaa ttgcatcgcg caggaattcg atctcttcct
      901 ccggcgccgg ggcgccgcga tagatgacct cgacgatttc ctcgaagcga aacagccaga
      961 acggcagttc gaacgaggcg gagtcgaccg tgcgcgccac gtcgcggaag gcgttggtgt
     1021 attcgttgtg ggggtcggca atgaccacgc gcaggccggg ccgtgtttcg atcgccgtgc
     1081 gcagcaggat cgacatcgaa caggatttgc cgacgccggt ggtgccgacc acggcgaaat
     1141 gcttcttcag catcccttcg acatcgaccg aggcggcgat atcggcatcc tgcgacagcg
     1201 tgccgatcgt tgagcccttg cgtccgccca gatcatagat ggccatcagg tcttcgcggc
     1261 ggatgcgatg ggccggcgag ccgatcggcg gatagaacga gatgccgcgg cggaattccg
     1321 gccgtccgtt gggcagatcg acgatctcgc ccagcagttc aacatccgcc gtgatcgggt
     1381 tgtcgccctg ctcctgccag gcgccatcgc gaaggcccat gtgcttgaca aagcagatca
     1441 cccggctgcg ctgaccatag acggtgatca atgtgccgac cgcccagtat ggcgcatcca
     1501 cgctcagcag ggcaggaaca cgcgtggcaa tggtggcgcc ggtgccatca atggcaacca
     1561 gcgttccgga ctggcgaaag ggcggctggt gggcctcgcg cctgtccgtt tcaacaggcg
     1621 ttccttggcc ggaatagatg acggtatgca tgcatgtccc cagggatcgg gtcactgtgc
     1681 caccgcatgg ttaaccaaat gtttggacag tggttttccc ggccactcga gggctcaggc
     1741 gctgaaactc tggacaagcg agccggcaac caggcgccaa ccgtccacaa ccacgaaaaa
     1801 caccagcttg aacggcagcg ccaccatcac cggcgggatc atcatcatgc ccatagaaat
     1861 gagaatcgag gcgacgatca gatcaatcac cagaaatggc aggaacagca ggaagccgat
     1921 ctcgaaggca cggcgcagct cggagatcat gaaggcggga accagagtcg tcagcggcag
     1981 ggcttccggt gtgtccggac gcggcttccg cgacaggctg acgaacagtt cgacatcctt
     2041 ttcccgcgca ttgcgcagca tgaatgcgtg gaacgggcgg accgtgcgct cgaatgcctc
     2101 gctctcgcgg atctcgccgg ccaccagggg tttgatcccg ccattgtagg ccgcctcgaa
     2161 cgtgggggcc atgacaaaag ccgtcagaaa cagcgacagg gcgatcagca ccatattggg
     2221 gggcgccgat tgcgtgccaa gggcgctgcg cagcagcgac agcgccacca cgatccgggt
     2281 gaacgaggtg gccatcacca ggatggatgg ggcgagcgcc agcacagtca gcaacgccat
     2341 cagatgcagg gcgcgctccg aaaggccggc cccaccaaga ttgaccgcaa tgtcctgcgc
     2401 ggccgcagga gtcgcaagcc ctgccaccag gggcagcagg gctatgaagg cgagggagct
     2461 gatcgtcttg cgatggatgc cgccgttcac gttttcccag ggccggagcg gcccaagagc
     2521 cgcgccatct cctcctccag cgaatcaatc gtctgagaat caggtgcgat cggcgctggc
     2581 ggaagatcct taccctgcgt caccggggcg ggggcgggct tttcgaaaga cttggcaggc
     2641 tttgccggct tggcaggcgg ttcctcggcg gctgcggcaa ccggttccgg gtccttggcc
     2701 cgctgcgctg cggacggcag gggaattggc ttggtctctg cctcggctgc cgccttggcg
     2761 tcaaccggct gggccggccg caatggcgac agcggacggt tcagcacgct ctgcaggcgg
     2821 cgcgccatgt ccgacagccg aggatcggcc gcagccggtc
//
LOCUS       DAOMXG010000228         2730 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_21219_length_2730_cov_10.875514, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000228 DAOMXG010000000
VERSION     DAOMXG010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21219_length_2730_cov_10.875514"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="PLQ11_11745"
     CDS             complement(<1..215)
                     /locus_tag="PLQ11_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020050821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRK25615.1"
                     /translation="MLVQLAIRDIVLIDRLDLAGATGLTALTGETGAGKSILLDAFAL
                     ALGARGDGALVERHRELAARDLRHRLIG"
     gene            complement(231..1118)
                     /locus_tag="PLQ11_11750"
     CDS             complement(231..1118)
                     /locus_tag="PLQ11_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HRK25616.1"
                     /translation="MMMRLGWSAGAARLLAAAALATLVAGCDTLEKVNPLNMFEDKGP
                     KSDIGPDQPAEVLFNDGLGRLEKKQYDAAAKKFAELDKQYPYSNWSRKALILTTFARY
                     ENGDYDEAVASGRRFVQLYPTDNDAAYAQYLVGMSYYNQIPDVTRDQDRTERALLALD
                     EVARKWPKSEYAADAKQRVMMARDQLAGKEMDIGRYYLKQRNYTGAVNRFREVLVRYQ
                     TTRHVEEALMRLAEAYMALGIVPEAQTAAAVLGHNFPDSPWYKDAYTLLQSGGTEPRE
                     SEGSWISKQFRGFTRTVGL"
     gene            complement(1339..2286)
                     /gene="lpxC"
                     /locus_tag="PLQ11_11755"
     CDS             complement(1339..2286)
                     /gene="lpxC"
                     /locus_tag="PLQ11_11755"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020094133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HRK25617.1"
                     /translation="MKQTTLGDRVVVDGVGVHSGRPARVILHPADANTGYLFLRTGLA
                     GGRERLISARFGSVKQTELCTIIGSDCGATVSTVEHCLAALAGLGVDNALIEIDGPEL
                     PILDGSSRVFIEAIDQVGIVPMAVNRKVVKVLKPVRVENGRSFAELLPGGAGLRLDVE
                     IDFDTPVVGRQARVFDLTPAGFRSEIAAARTFGFMKDVERLWKAGFALGASLENTVAI
                     GDGRVVNPEGLRFADEFVRHKILDAVGDLALAGHMIRGTYRAFCPGHKLNVMMLEELF
                     SDRSAYAIVDAVTSRREVGREVGYGELTAPQPAFGPTTR"
BASE COUNT          527 a          942 c          773 g          488 t
ORIGIN      
        1 cctatgagcc gatgccggag atcgcgagcc gcgagttccc gatgacgctc gaccagggcg
       61 ccatcgccgc gcgcacccag cgccagcgca aaggcatcca gcaggatcga cttgccggca
      121 ccagtctcgc cggtcagcgc cgtcagcccg gtcgcgccgg caagatcaag ccggtcgatg
      181 aggacaatat cgcggatcgc cagctggacc agcacggccg gcccccaagt ttacagaccg
      241 actgtgcggg tgaaaccccg gaactgtttg ctgatccacg aaccttcgct ttcccggggc
      301 tcggttccgc ctgattgcag cagcgtatag gcgtccttgt accagggact gtccgggaag
      361 ttgtggccga gaacggccgc tgccgtctgc gcttcaggca cgattcccag cgccatatag
      421 gcctcggcaa ggcgcatcag cgcctcttcc acatgccgcg tcgtctggta gcgcaccagc
      481 acctcgcgga agcggttgac tgcgccggta tagttgcgct gcttgagata gtagcggccg
      541 atatccattt ccttgccggc cagctgatcg cgcgccatca tcacccgctg tttggcatcc
      601 gccgcatatt cggacttcgg ccacttgcgc gccacctcgt cgagcgccag cagcgcccgt
      661 tcggtccgat cctgatcgcg cgtcacatcc gggatctggt tgtagtacga catgccgacc
      721 agatactgcg cataggccgc atcgttgtcg gtgggataaa gctggacgaa ccgccgcccg
      781 gacgcgacgg cctcatcata atcgccgttc tcgtagcgcg cgaaggtggt caggatcagc
      841 gccttccggg accagttcga atagggatac tgcttgtcga gctcggcgaa cttcttggcc
      901 gccgcgtcat actgcttttt ctcaaggcgg ccgagaccgt cgttgaacag cacttcagcc
      961 ggctgatccg ggccgatgtc gctcttcggc cccttgtcct cgaacatgtt gagcggattg
     1021 accttttcca gcgtgtcgca cccggccacc agcgtcgcca gggccgccgc cgccaacaat
     1081 cgcgccgcac cggcgctcca acccaaacgc atcatcatcc gcctaagctc aaccgcaccc
     1141 ttgaccaacc tgtcgcgcgc gacacttgca cggaaatcgc gtcttccgca actcgcctta
     1201 acgtcgagca tcgccggctg tcaccgacct cgccaccctc ctgtggtcgg cgaatatggc
     1261 aaagccacgg cgcggatccg ttcaaacgaa aatccccagg gattcagccc ggggatcgat
     1321 catgaaaaat gcgtccgctc agcgcgtcgt cggcccgaat gccggttgcg gcgccgtcaa
     1381 ttcgccatag ccaacctcgc gcccaacctc gcgccggctg gtcaccgcat cgacgatggc
     1441 gtaagcactg cgatcggaga acagttcctc aagcatcatc acattcagct tgtgtcccgg
     1501 gcagaaggcc cgatacgtgc cgcggatcat gtgtccagcc agcgccagat cgcccacggc
     1561 atcgagaatc ttgtgacgga cgaactcatc ggcgaagcgc aacccctccg ggttcaccac
     1621 ccggccatcg ccgatcgcca ccgtgttttc caggctggcg ccaagcgcaa accccgcctt
     1681 ccacagccgc tcgacatcct tcatgaagcc gaaggtgcgt gctgccgcga tctcggagcg
     1741 gaaaccggcc ggcgtcaagt caaacacccg cgcctggcgc ccgaccaccg gcgtatcgaa
     1801 atcgatctcg acatcaaggc gcaggccggc gccgcccggc agcagttccg cgaacgaccg
     1861 cccgttctcg acccgcaccg gcttgagaac cttgacgacc ttgcggttca ccgccatcgg
     1921 cacgatgccg acctggtcga tcgcttcgat gaaaacgcgc gacgacccat cgagaatcgg
     1981 caattcaggt ccatcgatct cgatcagcgc attgtcgacg ccgagaccgg ccagcgcggc
     2041 aaggcaatgc tcgaccgtcg aaaccgtggc accgcaatcc gacccgatga tggtgcacag
     2101 ttcggtctgc ttgaccgacc cgaatcgcgc actgatcagc cgctcgcgtc ctccggccag
     2161 gccggtgcgc aaaaacagat agccggtatt ggcatccgct ggatggagaa taacacgggc
     2221 tggacgccca ctgtggactc cgacgccgtc cactacaaca cgatcgccaa gcgtcgtttg
     2281 cttcacgtgc cccgtccttc gcttcggtat cctggccccg actggaactg agatacgtgc
     2341 aggaacctcg cgcggcgacg gactgacaga atcggtcaca caccgcgatt tcgtgacacg
     2401 gtgaatacct tcgtaaacgc cattggcaaa atcacgcttt ttaacccttt gtaacaaatg
     2461 cgacgaaaac ccaagtctct tgctagaata ttgtttttca ataacaaaac agaggccgga
     2521 aaccggcctc tgaatgcgtg tcgacgtttt gcgcagcacc gctgccaggc tcagttggcc
     2581 tgccgtctca gaaacgccgg gatttccagc gtctcgtcgg cattggcgcc ggcctgattg
     2641 gcaacgcggc cataggtgtc gagctggccg gccgccgcgc gcggggccgg cgccgccgaa
     2701 gtacgggtcg ggagcttcct tccccggcgt
//
LOCUS       DAOMXG010000229         2662 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_21971_length_2662_cov_9.806674, whole genome shotgun sequence.
ACCESSION   DAOMXG010000229 DAOMXG010000000
VERSION     DAOMXG010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21971_length_2662_cov_9.806674"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /gene="ruvA"
                     /locus_tag="PLQ11_11760"
     CDS             complement(<1..517)
                     /gene="ruvA"
                     /locus_tag="PLQ11_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HRK25618.1"
                     /translation="MIGKLKGVIDSYGEDFVILDVHGVGYVVYASTRTLQHLPPVGEA
                     AVLAIETQVREDAIRLFGFAADAERDWFRLLQTVQGVGSKVALAILSILKPNELATAI
                     ATSDKGMIGRASGVGPKLAARIVAELKDKAPAFGAVDPVLAKLAGDVEEKKAPGPVRD
                     AISALTNLGYGQ"
     gene            complement(514..1056)
                     /gene="ruvC"
                     /locus_tag="PLQ11_11765"
     CDS             complement(514..1056)
                     /gene="ruvC"
                     /locus_tag="PLQ11_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HRK25619.1"
                     /translation="MSAPMSPVRILGIDPGLRKTGWGMIAVEGTRVSFIACGSVEPDP
                     ALELAARLAALHEGLRQVVADHAPDEAAIEETFVNADARSALKLGAARGVAMLVPAQA
                     GLDVAEYAPNHVKKTVVGSGHAEKQQIRMMIKVLLPKAVVTSEDAADALAIALCHAHN
                     REARRRLNEVAALARRAGRS"
     gene            complement(1129..1875)
                     /locus_tag="PLQ11_11770"
     CDS             complement(1129..1875)
                     /locus_tag="PLQ11_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRK25620.1"
                     /translation="MAGHSQFKNIMHKKGKQDKIRSKIFSKLAREITVAAKMGMPDPA
                     MNPRLRLAVLAARAENMPKDNIQRAISKASGGEGENYDEIRYEGYGPGGTALIVEVMT
                     DNRNRTASDVRSAFTKAGGNMAETGAVSFMFDRVGVVEFDGDKASADAMLEAAIEAGA
                     DDVVSGQDGHEVYCEQSALNDVSKALEARFGEPRKSKLIWRPQNTISVDDEAGEKLMG
                     LIEKLEDHDDVQNVYSNFEISEALAARLSA"
     gene            complement(1969..2607)
                     /locus_tag="PLQ11_11775"
     CDS             complement(1969..2607)
                     /locus_tag="PLQ11_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009489823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HRK25621.1"
                     /translation="MLASLCGLSAAVTQAQTDTSLHAAVRSGDAAAVQRLIGSGAALD
                     ARDAQRQTPLLLAVQGSRSDITRLLIEAGADINAQASNQDTPWLLAGARGQADSLGLM
                     IPKGPDLAIRNRFGGNALIPACERGHVEAIRVLLTTRIDVNHINNLGWTCLLEIVILG
                     DGGRRHQEATRLVLAAGADPNIADKDGVRPLAHARARGQADVARLIEAAGGR"
BASE COUNT          440 a          932 c          809 g          481 t
ORIGIN      
        1 gctggccata accgagattg gtcagcgccg aaatggcatc gcgcaccggg ccgggcgcct
       61 tcttctcctc aacatcgccg gcaagtttgg ccagcaccgg atcgaccgcg ccgaaagccg
      121 gcgccttgtc cttgagttcg gcgacgatgc gcgccgccag cttcggcccg acccccgagg
      181 cccggccgat catgcccttg tcggacgtgg caatcgcggt cgcaagctcg ttcggcttga
      241 ggatcgacag gatcgccagc gcgaccttcg agccgacgcc ctgcaccgtc tgcaacaggc
      301 gaaaccagtc gcgttcggca tcggcggcaa agccgaacag ccggatagca tcctcgcgca
      361 cctgcgtctc gatggccagg accgccgcct cgcccaccgg tggcagatgt tgcagcgttc
      421 gcgtcgaggc atagaccaca tagccgacac catgcacgtc gaggatgaca aaatcctcgc
      481 cgtagctgtc gatcacaccc ttgagcttgc cgatcatgac cgccctgccc gccttgccaa
      541 agccgcaacc tcgttgagcc gccggcgtgc ctcgcgattg tgcgcatggc acaaggcaat
      601 cgccagcgca tccgccgcat cctcgctggt caccaccgcc ttgggcagca gcaccttgat
      661 catcatccgg atctgctgct tttcggcgtg ccccgatccg accaccgttt ttttcacatg
      721 attgggcgca tattcggcca cgtccagccc ggcctgcgcc ggcaccagca tggcgacgcc
      781 gcgcgccgcg ccgagtttca aggccgagcg cgcatcggca ttgacgaacg tctcctcgat
      841 cgccgcttcg tcgggcgcgt gatcggccac cacctgccgc agtccttcat gcagggcggc
      901 cagccgcgcc gccagttcca gcgccgggtc cggctccacc gagccgcagg cgatgaacga
      961 caccctcgtt ccctccactg cgatcattcc ccagccggtc ttgcgcaggc caggatcaat
     1021 gccgaggatg cggacggggc tcatcggagc ggacatgaac ccgcttatgg actgattccg
     1081 gccatgcggg ccaatcccgc agtgcgtcgt cgcccgtctg tagcccgatc aggctgacag
     1141 cctggctgcc agcgcctcgg aaatctcgaa gttggaatag acattctgca cgtcgtcgtg
     1201 atcttccagc ttctcgatca gccccatcag cttttcgccc gcctcgtcat cgacgctgat
     1261 ggtgttctgc ggccgccaga tcagtttcga cttgcgcggc tcgccgaacc gggcctccag
     1321 cgccttcgac acgtcgttga gcgccgattg ttcgcaatag acctcatgcc cgtcttggcc
     1381 ggagaccaca tcgtccgcgc cggcctcgat tgccgcttcc agcatcgcat cggcgctggc
     1441 cttgtcgcca tcgaactcga ccacgcccac ccggtcgaac atgaacgaga ccgcgccggt
     1501 ttcggccatg ttgccgccgg ccttggtgaa ggccgaacgg acatcggaag ccgtgcggtt
     1561 gcggttgtcg gtcatcacct cgacgatcag cgcggtgccg cccggcccgt agccctcata
     1621 gcggatctcg tcgtaattct cgccttcgcc gccgctcgcc ttggagatgg cgcgctggat
     1681 attgtctttc ggcatgttct cggcgcgtgc cgccagcacc gccaggcgca ggcgcggatt
     1741 catcgccgga tcgggcatgc ccatctttgc cgcgacagtg atttcgcgcg ccagcttgga
     1801 gaagatcttg gagcggatct tatcctgctt gcccttcttg tgcatgatgt tcttgaattg
     1861 gctgtggcct gccatgtgcc aaacctcgat cggaccaatg tgggaaagtt gggctggctt
     1921 ataggcacga cctgcgcaaa aggaaagaca ccagaggcaa ggcgtgcttc accgcccgcc
     1981 tgcggcctcg atcagccggg cgacatcggc ctgaccccgg gcccgggcat gggccagcgg
     2041 tcgcacccca tccttgtcgg cgatgttggg atcagccccg gcggccagca ccagtcgggt
     2101 ggcctcctga tgccgccggc cgccatcgcc gagaatcacg atctccagca ggcaggtcca
     2161 gcccagattg ttgatgtgat tgacatcgat cctggtggtc agcagcaccc ggatcgcctc
     2221 gacatgaccg cgctcgcagg cggggatcag cgcattgccg ccaaaccggt tgcggatcgc
     2281 aaggtccggc cctttcggaa tcatcaggcc gagactatca gcctgcccgc gcgcgccggc
     2341 gagcagccag ggcgtatcct gattgctcgc ctgtgcattg atgtcggcgc cagcctcgat
     2401 cagcagacgc gtaatatcac tgcggctgcc ctgcacggcg agcagcagcg gcgtctgccg
     2461 ctgggcatcg cgcgcatcga gcgccgcgcc ggaaccgatc aagcgttgga ccgctgccgc
     2521 atcgccggat cgcacagccg catgcagcga cgtgtctgtc tgggcctggg tcaccgcagc
     2581 ggagagcccg cacaggcttg ccagcacggt cgcgcatcga ggtagtggag cggaatccgg
     2641 ggatcgtgac ccgagggctg gt
//
LOCUS       DAOMXG010000230         2571 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_23104_length_2571_cov_7.467806, whole genome shotgun sequence.
ACCESSION   DAOMXG010000230 DAOMXG010000000
VERSION     DAOMXG010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23104_length_2571_cov_7.467806"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..484
                     /locus_tag="PLQ11_11780"
     CDS             98..484
                     /locus_tag="PLQ11_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25622.1"
                     /translation="MLRILNIAVLTLLVGTAAWAYQTKYETIFYAEQVKKLEARADKE
                     RDQIAILKAEWQLLNRPERLERLAQKHLDLKPIKAQQISRAEDLPDRKAAVDQIGAKL
                     DSLGITGSITPPADSSARKGSRPPGR"
     gene            513..2408
                     /locus_tag="PLQ11_11785"
     CDS             513..2408
                     /locus_tag="PLQ11_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRK25623.1"
                     /translation="MQRLVRVLDDLKQRLLSRRPAAFGLRTPVAPPAEAAARHREPFP
                     LPDFAAWRLSRPSLAGLLALGRDLFRTRPDKSTSRLVLAGFGFAVLFSVLGVRIVQFA
                     IRADDAPTFRRQAQEATSAARPPILDRNGELMATDVKMVSVFAEPRRIIDKDEAVELL
                     NAVFPDLDARELRDKLATKKGFIWVKREITPRQQAEVHRLGLPGVGFLPESKRVYPNG
                     PVAAHVLGFANVDNQGIAGIEKWIDGQGLNDLRGTGFASKPVDFKPIQLSIDLRVTHA
                     MRLELVEAMKKFKAKAAGGVIMDVNTGEIIAQVSLPDYDPNSPTDALKPENINHLAVG
                     VYEMGSTFKALTTAMALDSGKVSLTSNFDATRPLRYGRFAINDYHGQKRVLQVPEIFV
                     YSSNIGTARMALAVGVEGHKAFLRKLGQLERLRTEMPETAMPIIPAHWGELNTMTISF
                     GHGLAVSPLQATMATAALVNGGYLIAPTYLKRTREEAQALARPVIKPSTSEAMRYVMR
                     MNAERGTATKANIPGFYAGGKTGTSEKVVGGRYSKNKVMTTFMAVVPADKPRYLFLTL
                     LDEPQGISETHGYATSGWNAAPVTGRILERVGPMLDMTPRFELPNQPFPAVARLNVKY
                     QPWQEGR"
     gene            2410..>2571
                     /locus_tag="PLQ11_11790"
     CDS             2410..>2571
                     /locus_tag="PLQ11_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HRK25624.1"
                     /translation="MAVDAARLAAVLGLAADDAAGLTGACADSRAVRPGNVFFALAGA
                     KTDGSRFAAP"
BASE COUNT          466 a          838 c          819 g          448 t
ORIGIN      
        1 agctgcgcgc cgcaatccgc cccgaagccc ccgcccgcgc cgccgaagcg caggtcgtgg
       61 cgctttccga acttccgccc agacgaggga gacgctgatg ctgcgcatcc tcaacatcgc
      121 cgttctgacg ttactggtcg gcactgccgc atgggcctac cagaccaaat acgagacgat
      181 cttctacgcc gaacaggtca agaaacttga ggcacgcgcc gacaaggagc gggaccagat
      241 cgccattctc aaggccgaat ggcagctgct caaccggccg gagcggctgg agcggctggc
      301 gcaaaagcac ctcgatctca aaccgatcaa ggcccagcag atctcccgcg cggaggatct
      361 tccggatcgc aaggcggcgg tcgaccagat cggcgccaag cttgattcgc tgggaatcac
      421 cggctcgatc accccgccgg cagattcctc cgcccgcaag ggcagccggc ctccgggccg
      481 atagttgttt cccataacca tgggtcagtg gcatgcagcg tttggttcgc gttcttgacg
      541 atctgaaaca gcgtctcctg agccgccgtc cggcggcttt cggcttgcgc acgcccgttg
      601 ctccgccggc ggaagctgct gcgcgccacc gggagccgtt tccgctgccg gatttcgcgg
      661 catggcgcct gtcgcgtccc tctctcgccg ggctgcttgc cctgggccgc gacctgttcc
      721 gcacccggcc ggacaagagc acgagccggc tggttctggc cggattcggt tttgccgtgc
      781 tgttctcggt gctcggcgtg cggatcgtgc aatttgccat cagggcggat gatgcgccga
      841 ccttccgccg ccaggcgcag gaagcaacct ctgccgcgcg cccgccgatt ctcgatcgca
      901 acggcgaact gatggcaacc gatgtgaaga tggtctcggt gttcgccgag ccgcgccgca
      961 tcatcgacaa ggatgaggcg gtcgaactgc tcaatgcggt ctttcctgac ctcgatgcca
     1021 gggaattgcg ggataagctt gccaccaaga agggcttcat ctgggtcaag cgcgagatca
     1081 cgccgcgcca gcaggcggaa gtgcatcggc ttggcctgcc gggggtcggc ttcctgcctg
     1141 aatccaagcg cgtctatccc aacgggccgg ttgcggcgca tgtgctcggt tttgccaatg
     1201 tcgacaacca gggcattgcc ggtatcgaga aatggatcga cgggcagggg ctgaacgatc
     1261 tgcgcggcac cggctttgcc agcaagcccg tcgatttcaa gccgatccag ctgtcgatcg
     1321 atctgcgcgt cacccatgcg atgcggctcg aactggtcga ggcgatgaag aagttcaagg
     1381 ccaaggccgc cggcggcgtg atcatggatg tgaacaccgg cgagatcatc gcgcaggtat
     1441 cgctgcccga ttacgatccc aacagcccga ccgatgccct caagcccgag aacatcaatc
     1501 atctcgccgt cggcgtctat gaaatgggct cgaccttcaa ggcgctgacc accgcgatgg
     1561 cgcttgattc gggcaaggtg tcgctgacct cgaatttcga tgccacacgc ccgctgcgct
     1621 atggccgctt tgccatcaat gattaccacg gccagaagcg cgtgctgcag gtgccggaaa
     1681 tcttcgtcta ttcctccaac atcggcacgg cgcgcatggc gctggcggtg ggcgtcgagg
     1741 ggcacaaggc gttcctgcgc aagctcggcc agctcgaaag gctgcggacc gaaatgccgg
     1801 aaaccgcgat gccgatcatt ccggcccatt ggggcgaact caacaccatg acgatttctt
     1861 tcggccatgg tctggcggtt tcgccgttgc aggcgacgat ggcgacggcg gcgctggtca
     1921 acggcggcta tcttatcgca ccgacctatc tcaagcggac cagggaagag gcccaggcgc
     1981 tggccaggcc ggtgatcaag ccctcaacct cggaggccat gcgctatgtc atgcgcatga
     2041 atgccgagcg cggcacggcg acaaaagcca atattcccgg cttctatgcc ggcggcaaaa
     2101 ccggcacttc ggaaaaagtg gtcggcggcc gctattccaa gaacaaggtg atgaccacgt
     2161 tcatggcggt cgtgcccgcc gacaagccgc gctatctgtt cctgacgctg ctcgacgaac
     2221 cgcagggcat cagcgaaacg catggctatg cgacctcggg ctggaatgcg gcgccggtga
     2281 ccggccggat cctcgaacgg gtcgggccga tgctcgacat gaccccgcgc ttcgaactgc
     2341 cgaaccagcc gttcccggcg gtggcgcggc tcaatgtcaa ataccagccg tggcaggagg
     2401 ggcgctgaca tggcagtgga cgcggcgcgt cttgcggctg ttctcggtct cgcagcggat
     2461 gacgcggcgg ggctgacggg cgcctgcgcc gacagccggg cggtcaggcc gggcaacgtg
     2521 ttcttcgcgc ttgccggcgc caagaccgac ggcagtcgct ttgccgcccc a
//
LOCUS       DAOMXG010000231         2568 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_23143_length_2568_cov_9.505372, whole genome shotgun sequence.
ACCESSION   DAOMXG010000231 DAOMXG010000000
VERSION     DAOMXG010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23143_length_2568_cov_9.505372"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(173..1057)
                     /locus_tag="PLQ11_11795"
     CDS             complement(173..1057)
                     /locus_tag="PLQ11_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRK25625.1"
                     /translation="MQMLDLDQLRSLIAIADSGSFTRAAEIVCKTQSAVSMQMKRLED
                     QLARPIFAKEGRGTRLTEDGQRLLSYARRMVQINGECLATFADAVLTGRVRLGVPDDY
                     ADRYLPEILARFTRMHPKVEVTVVCEATPMLVERMQAGDLDLAIITLSFVRDLAEAIR
                     HERLLWVTSAHHSVHEQVPVPLALGRPNCAWRMVATERLEAAGKPFRVLYASWNSAAV
                     GAAVMSGLAVGVLPESAVRSGMRVLGPSDGFPPLPEVKIALLRSKLTSSAIIDALATQ
                     IVQSLDNLSGSGVAGTTL"
     gene            1189..1458
                     /locus_tag="PLQ11_11800"
     CDS             1189..1458
                     /locus_tag="PLQ11_11800"
                     /inference="COORDINATES: protein motif:HMM:NF018294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HRK25626.1"
                     /translation="MSNLSFSAPITAPAVFAVAKTTLVNAVAGLWQAWMHRRAVRQLQ
                     GFDARMLKDIGLGPTDVDAALSQPFTVDPSLHLSDVAAGRRPRRG"
     gene            complement(1548..>2568)
                     /locus_tag="PLQ11_11805"
     CDS             complement(1548..>2568)
                     /locus_tag="PLQ11_11805"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRK25627.1"
                     /translation="LPFILDAFGAEGVPRAVATRYLWIVLPSNFIMAAGMALSGLLRA
                     IGDARRAMMVTLIGGIATAFLDPLFIFTFKFGTDGAAIVTIIARLIMLWIGLVGVIGH
                     YQLLIRPTLADMRGHAGALAQVAVPAVLTNVASPVAAGFVLTVVRQFGHEAVAANTII
                     DRVVPLAFSVVFALSGSVGPILAQNLGAGLHARVRSTLRDSLLFALVYCLIVWAVLAM
                     GRDVIPAIFGASERTAEFIRFYCLIGVAAWIFNALLFVANAAFNNLGFPFYSMLFNWG
                     RASLGTLPFALTGAHLGGYPGLCVGLIIGWGIFGIAAVLTAFRTIARLERQAAPGVSS
                     GRGAG"
BASE COUNT          567 a          805 c          779 g          417 t
ORIGIN      
        1 ggccgaaggc ccggttgcga gggacggccc gggaaatgac cggcaggtca tttgtaaggg
       61 ccactggtat tattgagcat tttcaagcga agtggattcc ggttcgcgtc aagaaaatgc
      121 gaaaaaacaa aatgatagag cactttcagt gaattggaat tcactgaaag agctacagcg
      181 tggttccggc aacaccggac ccggacaggt tgtcgaggct ttgcacgatc tgcgtcgcca
      241 atgcatcgat gatcgccgat gaggtcagct tggagcgcag cagcgcgatc ttgacctccg
      301 gcagcggcgg gaaaccatcg ctcggtccca gcacacgcat gccggaccgc accgcgcttt
      361 ccggcaagac gccgaccgcc agcccgctca tcaccgctgc accgaccgcc gccgaattcc
      421 acgaggcata cagcaccctg aatggcttgc ccgctgcttc gagccgttcg gtcgccacca
      481 tccgccaggc acagtttggt cgtccgagcg ccagcggcac cggcacctgc tcatgcacgg
      541 agtgatgtgc cgaggtaacc cacagcagcc gttcatgccg gatcgcctcg gccagatcgc
      601 ggacgaatga cagcgtgatg atggcaaggt cgagatcacc tgcctgcatc cgctcgacca
      661 gcatcggggt cgcctcgcac accaccgtca cctcgacctt gggatgcatg cgggtgaagc
      721 gtgccaggat ctcgggaaga taacggtcgg cataatcatc gggaacgccc agccgcacgc
      781 ggccggtcag caccgcatcg gcaaaggtgg caaggcattc accgttgatc tgcaccatcc
      841 gccgcgcata gctcagcagg cgctgcccat cctcggtcag ccgtgttcca cgcccctctt
      901 tggcgaagat cggccgggca agctgatcct ccagccgctt catctgcatc gacaccgccg
      961 attgtgtctt gcagacgatt tccgcagcgc gggtgaacga acctgaatcg gcaatggcga
     1021 tcagggagcg aagctggtcg agatcaagca tctgcatggg acataatcca tcaacgaatg
     1081 acatgataac gatgaaaaca agtcatttca ataaggaaaa atgatatgtt agagacataa
     1141 tcaagcgcgg gcattctgcc ctgcgccgcc agcccaagga gatttgccat gtcgaacctg
     1201 tctttttccg ctccgatcac cgctcctgcc gtgttcgccg tggccaagac cacgctcgtc
     1261 aatgcagtcg ccggtctgtg gcaggcctgg atgcatcgcc gtgcggtgcg tcaattgcag
     1321 ggctttgacg cgcgcatgct gaaggatatc ggactcggtc cgaccgatgt ggatgccgcc
     1381 ctctcgcagc ccttcactgt cgatccctcg ctgcacctca gcgatgtggc ggctggccgg
     1441 cggccgcgcc gcgggtgatg cggtagagca tcatcccatc aagtggatgc cggttgatgg
     1501 ataagatgat gcgataaaac aaaccgctct tgaacgttac tgcctgatca accagcgccg
     1561 cgcccggacg atactccggg cgcggcttgc cgttccagcc gggcaattgt gcggaacgcg
     1621 gtgaggacgg cggcaatgcc gaatatgccc cagccgatga tcaggccgac acacaggccc
     1681 ggatacccgc caagatgggc gccggtcagt gcgaaaggca gcgtcccgag gctggcccgg
     1741 ccccagttga acagcatcga gtagaatgga aagcccaggt tgttgaaggc ggcattggca
     1801 acgaacagca gcgcattgaa aatccaggct gccacgccga tcaggcaata gaagcggatg
     1861 aactcagccg tgcgttcgct ggcgccgaaa atcgccggaa tgacgtcgcg ccccatcgcc
     1921 agaaccgccc agacgatcag gcaatagact agggcaaaca gcaggctgtc gcgcagggtg
     1981 ctacggaccc ttgcgtgcag gccggcgccg agattctgtg ccaggatcgg cccgactgag
     2041 ccggacagcg caaacaccac gctgaaagcc agcggcacca cccggtcgat gatcgtgttg
     2101 gcggccaccg cttcatgccc gaactgccgc accacggtca gcacgaaacc cgccgccacc
     2161 gggctggcga cattggtcag cacggcagga acggcaacct gggccagcgc accggcatgg
     2221 ccgcgcatgt cggcaagggt cggccggatc agcaattgat aatgcccgat gacgccgaca
     2281 agaccgatcc acagcatgat caaccgggca atgatggtga cgatcgccgc gccatcggtg
     2341 ccgaacttga aggtgaagat gaaaagcggg tcgagaaacg ccgtggcgat gccgccgatc
     2401 agcgtcacca tcatcgctcg gcgcgcatcg ccgatggcac gcagcagacc cgacagcgcc
     2461 atgcctgccg ccatgatgaa attggatggc agcacgatcc acagatagcg ggtggcaact
     2521 gcgcgcggca cgccctcggc gccgaaggca tcgaggatga acggcagc
//
LOCUS       DAOMXG010000232         2544 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_23437_length_2544_cov_8.127762, whole genome shotgun sequence.
ACCESSION   DAOMXG010000232 DAOMXG010000000
VERSION     DAOMXG010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23437_length_2544_cov_8.127762"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="PLQ11_11810"
     CDS             <1..759
                     /locus_tag="PLQ11_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRK25628.1"
                     /translation="RIGVAPHSLRAVTGDELTALAGVFRAGPIHIHVAEQIREVEESL
                     AFSGRRPVEWLLDHAEVDGRWCLIHATHMTEAETLAVATSGAVAGLCPITEANLGDGL
                     FPAPEFLAAGGRFGVGSDSNVEIGAAAELRLLEYGQRLSRRGRNLLADREGQSTGEAL
                     YRAALAGGAQALGQPVGALAPGCHADLVVLDVRHPALAALDAADWLDGYIFAGGAAAI
                     DKVVVRGQLVVDGGRHVHRSAVAARFAERMRRLQ"
     gene            756..1505
                     /locus_tag="PLQ11_11815"
     CDS             756..1505
                     /locus_tag="PLQ11_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009489353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HRK25629.1"
                     /translation="MNAPLPLHIHQRIRADIEGHILAGRWPPGHAIPSEHDLMVTYGC
                     SRMTVNKVLSSLAAQGVINRRRRAGSVVARPSVDKAVLTIRDFPLEAARAGKTYAFEL
                     LAHGIERLDEAAARRNGLAVGTPVRQVTTLHRFDGAPEALEERLINLDLAPGARDELF
                     CDEPPGTWLLQRVPWSDAEHVIRAVNASPTLAKRLGLAAGTACLVIERRTWQGAVLVT
                     EARLYYPGEQHRLVGRFTSTGTTGASSGPVS"
     gene            1574..2518
                     /locus_tag="PLQ11_11820"
     CDS             1574..2518
                     /locus_tag="PLQ11_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRK25630.1"
                     /translation="MKSAILAGLFTATTLLTPALAQETKVTIGISGWTGFAPLTLAKE
                     AGIFKKNGLDVTIKKIPQASRHLAIQSGDVQCAATTVETWVVWNANGVPTKQIFQLDK
                     SYGADGMAVRNTIGSIKELKGKTVAASAPGTAPYFTLAWMLKKNGLSVKDVTVVNLEP
                     GPAAQAFVAGQNDAAMTYEPYLSTVRAAPDKGKIIATTLDYPMIMDTFGCTPKFLDEN
                     GKAAKALADSYFEALEMINKEQAKAYEIMGADVKQTGEAFGNSAKYLRWQDRAANRKF
                     FEAEHAAFSNEAADLLLEIGIIKQKPDIKSLADTRFIQ"
BASE COUNT          444 a          809 c          848 g          443 t
ORIGIN      
        1 cggatcgggg ttgcgccgca ttcgctgcgc gcggtcaccg gcgatgaact caccgcgctg
       61 gccggcgtat ttcgcgctgg tcccatccac atccatgtcg ccgaacagat ccgcgaggtc
      121 gaggaaagcc tcgccttttc cggccgccgt ccggttgaat ggctgcttga ccatgccgag
      181 gtcgatggcc gctggtgcct gatccacgcc acgcatatga ccgaggcgga aaccctggcg
      241 gttgccacca gcggcgcggt tgccgggctg tgtccgatca ccgaggccaa tctgggcgat
      301 gggttgttcc cggcgccgga atttcttgcc gccggcggcc gtttcggggt cgggtccgat
      361 tccaatgtgg aaatcggggc tgccgccgaa ctgcgtctgc tggaatacgg gcaaaggctc
      421 agccgtcgcg gccgcaatct tctggctgac cgagagggcc agtcgaccgg agaggcgctt
      481 tatcgggcgg cgctcgccgg cggcgcgcag gcattgggcc agccggtcgg tgcattggcg
      541 ccgggctgcc acgctgatct tgtcgtactg gatgtccgcc atccggcgct ggccgcgctt
      601 gacgctgccg actggctcga cggctatatt tttgctggcg gtgcggccgc aatcgacaag
      661 gtggtggtgc gcggacagct ggttgttgat ggcgggcggc atgtgcatcg cagcgccgtt
      721 gccgcccgct ttgccgagcg gatgcggagg ctgcaatgaa cgcgccgctg ccgctgcata
      781 tccatcagcg tatccgcgcc gacatcgagg gtcacattct cgccggccgc tggccgccgg
      841 gtcatgcgat cccctccgag catgacctga tggtgaccta cggctgctcg cgcatgaccg
      901 tcaacaaggt gctgtcctcg ctcgcagcgc agggggtaat caaccgccgt cgccgggcgg
      961 ggtcggtggt ggcgcggccg agcgtcgaca aagcagtgct gaccatccgc gattttccgc
     1021 tggaagcggc gcgcgccggc aagacctatg cgttcgaatt gctcgctcat ggtattgagc
     1081 ggctggatga agccgcggcg cggcgcaacg gtcttgctgt cggcacgccg gtgcggcagg
     1141 tgacgaccct gcatcgcttt gatggcgcgc ccgaagcgct cgaagaacgg ttgatcaatc
     1201 tcgatctcgc tcccggagcg cgcgatgaac tgttttgcga tgaacctccc ggaacctggc
     1261 tgctgcaacg ggtgccttgg agtgatgccg aacatgtcat ccgcgccgtc aatgccagcc
     1321 cgacgcttgc caagcgcctc gggcttgctg ccggaacagc ctgtctggtg atcgagcggc
     1381 gcacctggca gggggccgtg ctcgtgaccg aggcgcgtct ctattaccct ggcgagcagc
     1441 accggctcgt cgggcggttt acctcgaccg gcacgacggg cgcgtcgtcc ggtccggttt
     1501 cctgaagggc agagcgttct gcgctgcccg ccatcatcaa caccggctgc ggccggaaac
     1561 aggggagtgg gacatgaaat cagcgattct ggcaggactt ttcacggcta caacgctgct
     1621 gacgccggcg ctggcgcagg agaccaaggt caccatcggc atttccggct ggaccggatt
     1681 tgcgccgctg acgctggcca aggaagccgg catcttcaag aagaacgggc ttgatgtgac
     1741 gatcaagaag ataccgcagg cgtcccgcca tctcgccatc cagtcgggcg atgtgcaatg
     1801 cgccgcgacc accgtggaga cctgggtggt gtggaacgcc aacggtgtgc cgaccaaaca
     1861 gatcttccag ctcgacaaga gctatggcgc cgacggcatg gccgtgcgca acaccatcgg
     1921 ctcaatcaag gagctgaagg gcaagaccgt ggcggcttct gcgcccggca ccgcgcccta
     1981 cttcaccctg gcctggatgc tgaagaaaaa cggcctcagc gtgaaggacg ttaccgtggt
     2041 caaccttgag cccggcccgg cggcgcaggc gttcgtcgcc ggccagaacg atgcggcgat
     2101 gacctacgag ccttatctgt cgactgtccg cgccgcgccg gacaagggca agatcatcgc
     2161 cacgacgctc gactatccga tgatcatgga cacgttcggc tgtacgccga aattcctcga
     2221 tgagaacggc aaggccgcca aggcgctcgc cgacagctat ttcgaggcgc tggagatgat
     2281 caacaaggag caggccaagg cctatgagat catgggcgcc gacgtgaagc agacgggcga
     2341 agccttcggt aattctgcca aatacctgcg ctggcaggat cgcgccgcca acaggaagtt
     2401 cttcgaggcc gagcatgccg ccttctcgaa cgaggcggcg gacctgctgc tcgagatcgg
     2461 catcatcaag cagaagcccg acatcaagag cctcgccgac acccgcttca tccagtaacc
     2521 cgatgggggc cggcggcagc gccg
//
LOCUS       DAOMXG010000233         2356 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_26190_length_2356_cov_10.358105, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000233 DAOMXG010000000
VERSION     DAOMXG010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26190_length_2356_cov_10.358105"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /locus_tag="PLQ11_11825"
     CDS             <1..1071
                     /locus_tag="PLQ11_11825"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRK25631.1"
                     /translation="IGQPDFATPPNIVEAAIRALRDGHHGYTPATGILPLREAVATDL
                     HRRFGASVSPERVLIVPGGKVTMFMAILMFGAPGVEILYPDPGFPIYRSMIEFTGATP
                     VPVSIREENGFAFSAEETLALITPRTRLLIINSPANPTGGVTPKAEVDRLVAGLQRFP
                     RVAVMSDEIYDQMLYDGEQHVCLLNYPEIADRLILLNGWSKTYAMTGWRMGYSVWPQA
                     AYEAARKLAVNSYSCVNAAAQWAGLEALTGPQDAVGTMLKEFDRRRKAVVAGLNTLPG
                     VSCITPKGAFYAFPNVKATGWKAKPLASALLEQTGVATIGGPDFGVHGEGYIRLSYAN
                     SLDNIEKALARIGAFLDKNRKA"
     gene            1068..1517
                     /locus_tag="PLQ11_11830"
     CDS             1068..1517
                     /locus_tag="PLQ11_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRK25632.1"
                     /translation="MTIRQATAADLPAILKLIADDPLGKTREAQADEHAAYQAAFAAI
                     AADPNNLAVVMEEAGILVGYFQLTFIPGLSRRGAWRGQIESVRIATSCRNRGYGEAMM
                     RWAIEECRRRGCTLVQLTTDKRRPAAHRFYERLGFIASHEGMKLDLT"
     gene            complement(1569..>2356)
                     /locus_tag="PLQ11_11835"
     CDS             complement(1569..>2356)
                     /locus_tag="PLQ11_11835"
                     /inference="COORDINATES: protein motif:HMM:NF016291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF533 domain-containing protein"
                     /protein_id="HRK25633.1"
                     /translation="TAAPGGLGDLVGSILGQAAGGARDAARDANAATGASDMLGQLIE
                     QATGKKLSPDMLNQIQEMIANNQLAAGAAAGGLGGLLLGTKSGRNITATAAKLGGMAL
                     IAGLAYKAYNNYIQGKPLIKTGEAVTLPPKNSAFHPDAGDVNQNATLMLRGMIAAAMS
                     DGKLEEAEKKVILGSLEARGLDQDAVDFINQEIAQPADIASLAEGVQGPEQATALYTA
                     CRLAIDLNNKYEKRFMEDLAEALGLDDVLKQQVESAAVSVRPA"
BASE COUNT          393 a          818 c          718 g          427 t
ORIGIN      
        1 atcggccagc cggatttcgc cacgcccccc aacatcgttg aggccgccat cagggcgctg
       61 cgcgacggcc atcacggcta caccccggcg accggcatcc tccccctgcg cgaggccgtc
      121 gccaccgatc ttcaccgccg cttcggcgcc tccgtcagcc ccgaacgggt gctgatcgtg
      181 cccggcggca aggtgacgat gttcatggca atcctgatgt ttggcgctcc gggcgtcgag
      241 atcctctatc cggaccctgg atttccgatc tatcgctcga tgatcgaatt taccggcgcg
      301 accccggtgc ctgtgtcgat ccgcgaggaa aacggctttg ccttctcagc cgaggaaacg
      361 ctggcgctga tcaccccgcg cacgcggctg ctgatcatca attccccggc caacccgacc
      421 gggggcgtca cgccgaaggc ggaggtggac aggctcgtcg ccggcttgca gcgtttcccg
      481 cgcgttgccg tgatgtcgga cgagatctac gaccagatgc tctatgatgg cgagcagcac
      541 gtctgcctgc tcaactaccc cgaaatcgcc gaccggctga tcctgctcaa cggctggtcc
      601 aagacctatg cgatgaccgg ctggcgcatg ggctattcgg tgtggccgca ggcagcctat
      661 gaggctgccc gcaagctcgc cgtcaattcc tattcctgcg tcaacgccgc cgcgcaatgg
      721 gcggggctcg aagcgttgac cggcccgcag gatgccgtgg ggaccatgct gaaagagttc
      781 gaccgccgcc gcaaggcggt cgttgctggt ctcaacaccc ttccgggcgt ctcctgcatc
      841 acgccaaagg gcgctttcta cgccttcccg aacgtcaagg cgaccggctg gaaagccaag
      901 ccgctggcct ccgccctgct ggagcaaacc ggggtcgcca ccatcggcgg accggacttc
      961 ggcgtgcacg gcgagggcta catccgtctg tcctacgcca acagcctcga caacatcgag
     1021 aaggcgctgg cgcgcatcgg cgcatttctg gacaagaacc gtaaggcatg accatccgcc
     1081 aggccaccgc cgccgatctg cctgcgatcc tcaaactcat tgccgacgac ccgctcggca
     1141 agacccgtga ggcgcaggcg gacgagcacg ccgcctacca agccgccttc gccgcgatag
     1201 ctgccgatcc caacaatctg gccgtcgtca tggaggaggc cggcatcctt gtcggctact
     1261 ttcagctgac cttcatcccc ggcctgtcgc gcaggggcgc ctggcgcggc cagatcgaga
     1321 gcgtcaggat cgccacctcc tgtcgcaatc gcggctacgg cgaggccatg atgcgctggg
     1381 cgatcgagga atgccggcgg cgcggctgca cgctggtgca actcacaacc gacaaacgcc
     1441 gtccggctgc ccaccgcttt tatgaacggc tcggattcat cgccagccac gagggcatga
     1501 agctggacct cacatgaaaa acggcgggtt gccccgccgt ttcaattccc tgatttggcg
     1561 cccgcctctc aggccggacg caccgagaca gcggccgatt cgacctgctg cttgagcacg
     1621 tcatcaagcc cgagcgcctc ggcgagatct tccatgaagc gcttctcgta tttgttgttg
     1681 aggtcgatgg cgaggcggca ggcggtatag agtgccgtcg cctgctccgg tccctgcacc
     1741 ccttcggcca gcgaagcgat gtcggcaggc tgagcgattt cctggttgat gaaatcgact
     1801 gcatcctgat ccaggccacg cgcttccaga ctgcccagga tgaccttctt ctcggcttcc
     1861 tcgagtttgc catccgacat ggcggccgca atcatgccgc gcagcatcag cgtcgcgttc
     1921 tggttgacat cgccggcatc agggtggaac gccgaattct tgggcggcag cgtcaccgcc
     1981 tcgcccgtct tgatcagcgg cttgccctga atgtagttgt tgtaggcctt gtaggcgagc
     2041 ccggcgatca gcgccatgcc gccaagtttg gctgccgtgg cggtaatgtt ccgacccgac
     2101 ttggtgccga gcagcagccc gccgaggccg cctgccgcag cgccggcggc aagctggttg
     2161 ttggcgatca tctcctggat ctggttcagc atgtcgggcg aaagcttctt gccggtcgcc
     2221 tgttcgatca actggccgag catatccgag gcacctgtcg ccgcattggc atcgcgcgct
     2281 gcatcgcgtg cgccgcccgc tgcctgaccg aggatcgagc cgacgaggtc gcccagccca
     2341 cccggcgccg ccgtct
//
LOCUS       DAOMXG010000234         2117 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_30560_length_2117_cov_9.098933, whole genome shotgun sequence.
ACCESSION   DAOMXG010000234 DAOMXG010000000
VERSION     DAOMXG010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30560_length_2117_cov_9.098933"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="PLQ11_11840"
     CDS             complement(<1..547)
                     /locus_tag="PLQ11_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007565388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HRK25634.1"
                     /translation="MNSNFRNFGIWIVILLLVFALFTVLQGQGGRSVGADTPYSRFLD
                     DLDAGKIARVTIARDEITGMTTDGRNFQTRAPFDPKLVERLHEKKIEFNVVPPNTDSP
                     WYYQLLFNWLPLIVFLGAWIFMSRQMQNGAGRAMGFGKSKAKLLTEAHGRVTFDDVAG
                     IDEAKEDLQEIVEFLRDPQKFQ"
     gene            complement(654..1673)
                     /gene="tilS"
                     /locus_tag="PLQ11_11845"
     CDS             complement(654..1673)
                     /gene="tilS"
                     /locus_tag="PLQ11_11845"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HRK25635.1"
                     /translation="MRGLDAEPVTQAEAAVLFGRFDPLESVALAVSGGPDSTALLGLW
                     AGCRRGRGDVVVTVDHGLRPEARGEAEAVGRFARRLGLEHVVLTWDGDKPASGVQEAA
                     RRARYSLMRAFVQARGLQGVVLAHTRDDQAETVLMRLVRGSGLRGLAGMTEFSNLDGL
                     SLLRPLLAVSKARLVASCEANGWPFIRDPSNENAAFLRPRLRRLMPLLAAEGLTAERL
                     SVLAERAAEADFLLDHVARSALIDSDAALRGRKAGYGAVHAPTMLGKGREKAVRMLSL
                     AIRGYDPEAVPVLLLERLEALAERLLAAFEAGRPHTGTIAGYKVRLEGDIVRISPAPP
                     RRRLS"
     gene            complement(1661..>2117)
                     /gene="ybgF"
                     /locus_tag="PLQ11_11850"
     CDS             complement(1661..>2117)
                     /gene="ybgF"
                     /locus_tag="PLQ11_11850"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HRK25636.1"
                     /translation="PQPLQPGNGTAAQPPVAALPTAGTPKGDFDIASAVLDRGELDAA
                     EAAFRDFVKKYPKDKLKPDALFNLGESFYRRNRYREAAEQYLVITTEHGQSGRAAEAM
                     LKLGMSLRGLGATAEACGTYGEVGKKYPKASAAVKQAVEREKKRAQCAA"
BASE COUNT          374 a          731 c          650 g          362 t
ORIGIN      
        1 gctggaactt ctgcggatcg cgcaggaact cgacgatctc ctgcaggtcc tccttggcct
       61 cgtcgatgcc ggcgacatca tcaaacgtca cgcgtccatg cgcctcggtg agcagcttgg
      121 ctttggactt gccaaaaccc atcgcccgcc cggcgccatt ctgcatctgc cgcgacatga
      181 agatccaggc gccgagaaac acgatcagcg gcagccagtt gaacagcagc tggtaatacc
      241 acggcgaatc ggtattcggc gggacgacat tgaattcgat tttcttctca tgcagccgct
      301 cgaccagctt cggatcaaac ggtgcccgcg tctggaaatt gcggccatcg gtggtcatgc
      361 cggtaatttc atcgcgtgca atcgtcaccc gcgcgatctt gccggcgtca aggtcgtcaa
      421 ggaaacggct gtagggcgta tcggcgccga ccgaccttcc gccctggccc tgcagcacgg
      481 tgaacagcgc gaacaccagc aggaggatga cgatccagat gccgaagttg cggaaattcg
      541 agttcatgac ctatccagac atggttgggc ggcgcagccg acgcgcgcga tcataagcct
      601 atttaggcgc gctgtgctcc cttgccaagc tatcggcgcc tgatcccgtg tgattagctt
      661 aaccgcctgc gcggcggggc aggagaaatg cgcacgatat cgccttcaag ccgcaccttg
      721 tagccggcga tggtgcccgt atgcggccgg ccggcttcga aggccgcgag caaccgctcg
      781 gccagcgcct ccagccgctc cagcagaagg acgggtactg cttcgggatc gtagccccgg
      841 attgccaggc tgagcatgcg taccgctttc tcgcgcccct tgccaagcat ggtcggcgca
      901 tggaccgcgc catatccggc cttgcgcccc cgcagggcag cgtcactatc gatgagggcc
      961 gatcgggcga catgatcgag caggaaatcg gcctcagccg cccgctctgc cagcaccgaa
     1021 agccgctctg cggtcagccc ttcggcagcg agcagcggca tcagccggcg caggcgcggc
     1081 cgcagaaaag cggcgttctc gttgcttgga tcgcggataa acggccagcc attggcctcg
     1141 cagctcgcca ccaggcgcgc cttgctcacc gccagcagcg gtcgcaacag ggacaggccg
     1201 tcaaggttgg agaattccgt cattccggcc agccctcgca ggcctgaccc gcgcaccagc
     1261 cgcatcagca cggtttccgc ctgatcgtcg cgcgtatggg cgaggacaac gccttgcaac
     1321 ccgcgcgcct gcacgaaggc acgcatcagg ctatagcgcg cccgccgcgc cgcctcctgc
     1381 acaccgctgg ccggcttgtc gccgtcccag gtcagcacga catgttcgag ccccagccgg
     1441 cgggcaaaac gccccacagc ctcggcctca ccgcgtgcct cggggcgcag gccatgatca
     1501 accgtgacca ccacatcgcc gcgtccgcgc cggcaaccgg cccagaggcc cagcaacgcc
     1561 gtggaatcgg gaccgccgga aaccgccaga gcgacagatt cgagaggatc gaagcgcccg
     1621 aacagcaccg ccgcttcggc ttgggtgact ggctcggcgt ctaggccgcg cattgggcgc
     1681 gcttcttttc gcgctcgacc gcctgtttca ccgccgcaga cgccttggga tacttcttgc
     1741 cgacttcacc ataggtgccg caggcttccg ccgtggcccc caggccgcgc aggctcatgc
     1801 ccagcttcag catcgcttcg gccgcccggc ccgattggcc atgttcggtg gtgatcacca
     1861 gatactgctc cgccgcctcg cgatagcggt tgcggcgata gaagctctcg ccgagattga
     1921 acaaggcatc cggcttgagc ttgtccttgg gatatttctt cacgaagtca cggaaggctg
     1981 cttcggccgc atcgagttca ccgcgatcca gcacggcgct ggcaatatcg aagtcgccct
     2041 tgggtgttcc ggccgtcggc agcgcggcaa ccggtggctg ggcagcggtg ccgttccccg
     2101 gttgcagcgg ctgtggc
//
LOCUS       DAOMXG010000235         2009 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_32957_length_2009_cov_8.846469, whole genome shotgun sequence.
ACCESSION   DAOMXG010000235 DAOMXG010000000
VERSION     DAOMXG010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32957_length_2009_cov_8.846469"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="PLQ11_11855"
     CDS             complement(<1..733)
                     /locus_tag="PLQ11_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HRK25637.1"
                     /translation="MLRTTLLAAAAILPMTATATLAQGAKLVAIDGSSTVFPVMEAVA
                     EEFQKAKKGQVRVTVGISGTGGGFKKFCRGETDISNGSRPILKSEIEDCQKSGVEFFE
                     LPLAFDALTVAVSPKNTFLSCVTTEQLKKIWEPAAQGKVTKWKDVDPSWPDAPMKLFG
                     AGADSGTFDYFTEATVGKAKSSRGDFTASEDDNVLVQGIEHDANSLGYIPFAYYAPHA
                     DKMKALAVDWGKGQGCVKPSLENVLA"
     gene            1017..>2009
                     /locus_tag="PLQ11_11860"
     CDS             1017..>2009
                     /locus_tag="PLQ11_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019965845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRK25638.1"
                     /translation="MRCSLVRILCITLWLGALGAKAQAQSAGREAPSSRAEVTLSFAP
                     VVKKAGPAVVNVYGERREQQARHPLLDDPFFRELFGGRLPRERVQQSVGSGVIVGEDG
                     IVVTNHHVIEGMTQVKVSLADRREFEADILLRDPKTDLAVLKIKARETFPALELGDSD
                     ALEVGDIVLAVGNPFGVGQTVTQGIISALARTHVDVSDYQFFIQTDAAINPGNSGGAL
                     VDVKGQVIGINTAIYSKSGGSHGIGFAVPANMVRVVVASARTGAQKVVRPWYGAKMQP
                     VGAEMVDSLGLDRPVGALVASVVKDSPAAVSGLKAGDVIVAVDGVAIDDPDAFGF"
BASE COUNT          344 a          605 c          657 g          403 t
ORIGIN      
        1 cggccagcac gttttcgagc gagggtttga cgcagccctg acccttgccc cagtcgaccg
       61 ccagcgcctt catcttgtcg gcatgcggcg cgtaataggc gaagggaatg tagccgagcg
      121 aattggcgtc gtgttcgatc ccctgcacca gcacattgtc atcttccgaa gcggtgaagt
      181 cgccgcggct tgacttggcc ttgccgaccg ttgcctcggt gaaatagtcg aacgtgcccg
      241 aatcggcgcc ggcgccaaac agcttcatcg gcgcatcggg ccaggacgga tcgacgtcct
      301 tccacttggt caccttgccc tgcgcggcgg gttcccagat cttcttgagc tgttcggtgg
      361 tcacgcagct caggaaggtg ttcttcgggc tgacggcgac ggtcagcgcg tcgaaggcca
      421 gcggcagttc aaagaactcg acgccgctct tctgacaatc ctcgatctcg gacttgagga
      481 tcgggcgcga gccgttggaa atgtcggtct cgccgcggca gaatttcttg aagccgccgc
      541 cggtgccgga aatgccgacc gtgacgcgga cctggccctt cttggccttc tggaattcct
      601 cggcaacggc ctccatcacc gggaacaccg tcgacgaacc gtcgatggcg accagttttg
      661 ctccctgggc cagcgtggcg gtcgccgtca tcggcaggat cgccgcagcg gcaagaaggg
      721 tcgttctcag catcgttctg tctccgtggg gtcgccggcg gaccagcgag gtgtcactgg
      781 tgttgcccta ggggcgtttc atgacagctt gatgacatgg gttgcaggag ccgtaagacc
      841 aagtcgcaag gcgaggaact cgccgcgact tggttcgttt caaaaatttc tcatttttat
      901 caatgagatg agaaatttgt gagttgcacc aaagagtcat tttcaatgac tttttggtat
      961 gagacttggt gcatcaggtg tgctaccccg atttcgtctt atccccagga cctgccatgc
     1021 gctgttctct cgtccgcatc ctgtgcatca ccctctggct cggcgcgctc ggagcgaagg
     1081 cacaggcaca atcggcaggc cgcgaggcac ccagcagccg cgccgaggtg accctgtcct
     1141 tcgccccggt ggtgaagaag gccggaccgg cggtggtcaa tgtctatggc gaacggcgcg
     1201 agcagcaggc gcggcatccc ttgctcgatg atccgttctt ccgcgaactg ttcggcgggc
     1261 ggctgccgcg cgaacgggtg cagcaatcgg tcggctccgg cgtcatcgtc ggcgaggatg
     1321 gcatcgtcgt caccaaccat catgtcattg agggtatgac tcaggtgaag gtgtcgctgg
     1381 ccgaccggcg tgagttcgag gccgatatcc tgctgcgcga tccaaagacc gatctagccg
     1441 tgctgaaaat caaggcgcgc gagacattcc ccgcgctgga gctgggcgat tccgacgcgc
     1501 tggaagtcgg cgacatcgtg ctggcggtcg gcaatccgtt cggcgtcggc cagacggtga
     1561 cgcagggcat catctcggcg ctggcgcgca cccatgtcga tgtgtcggac taccagttct
     1621 tcatccagac cgatgcggcg atcaaccccg gcaattcggg cggcgcgctg gtcgatgtga
     1681 aggggcaggt gatcggcatc aatacggcga tctattccaa atcggggggc agccatggca
     1741 tcggctttgc cgttccggcc aacatggtgc gggtggtggt ggccagcgcc cgcaccggcg
     1801 cccagaaggt ggtccggccc tggtatggcg ccaagatgca accggtgggc gccgaaatgg
     1861 tcgacagtct cggcctcgac cggccggtgg gggcgctggt cgccagcgtt gtcaaggaca
     1921 gcccggccgc cgtctcgggc ctcaaagccg gcgatgtgat cgttgcggtc gacggggtgg
     1981 cgatcgacga tccggatgcc ttcggtttc
//
LOCUS       DAOMXG010000236         1777 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5658
            NODE_39249_length_1777_cov_15.772938, whole genome shotgun
            sequence.
ACCESSION   DAOMXG010000236 DAOMXG010000000
VERSION     DAOMXG010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867879
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.59
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:13:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,376
            CDSs (total)                      :: 2,340
            Genes (coding)                    :: 2,331
            CDSs (with protein)               :: 2,331
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39249_length_1777_cov_15.772938"
                     /isolate="AMR_MDS_5658"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..749)
                     /gene="leuD"
                     /locus_tag="PLQ11_11865"
     CDS             complement(144..749)
                     /gene="leuD"
                     /locus_tag="PLQ11_11865"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HRK25639.1"
                     /translation="MQPFTKLTAVAAPMKVVNVDTDMIIPKQYLKTIKRTGLGKALFS
                     EMRYREDGGENPDFVLNKPAYRNAQILVAGDNFGCGSSREHAPWALLDFGIRCVISTS
                     FADIFYNNTFKNGILPIKVSQAELDKLFDDAERGANSTLTIDLEAQEIRGPDGGVIRF
                     DIDPHRKHCLINGLDDIGQTLQKVGSIDAYEARAAAARPWA"
     gene            complement(848..1267)
                     /locus_tag="PLQ11_11870"
     CDS             complement(848..1267)
                     /locus_tag="PLQ11_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HRK25640.1"
                     /translation="MTDQALPWPAARAPSLETFEVMAAAAFDRLPAGFRKLCEGVIIQ
                     VADFPDDETMDDMQLETPFDILGLFRGIGLAQAPAMMETGQFPNMIWLYRRPILDYWA
                     EHDETLGKIITHVLVHEIGHHFGLSDADMEAIEAQVS"
     gene            complement(1272..1400)
                     /locus_tag="PLQ11_11875"
     CDS             complement(1272..1400)
                     /locus_tag="PLQ11_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25641.1"
                     /translation="MQTGSDDDNDRIVILAKRIGRTLGYLFVGYLIFSLGQLARLW"
     gene            1588..>1777
                     /locus_tag="PLQ11_11880"
     CDS             1588..>1777
                     /locus_tag="PLQ11_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRK25642.1"
                     /translation="MDRRHLIAGGAGLLLAGLFAGSASARITAQGPVADEAGPQVEDV
                     KRGGRGRHRRPAAQAARAG"
BASE COUNT          345 a          519 c          570 g          343 t
ORIGIN      
        1 caaatgggaa tcccaaatcc ccacgcgcct tagagcatgt cgcgattaag tggataccgg
       61 tcaatcgctg aagacatgct ccaacttatt gataccgatc acgttatccg cattttgatg
      121 attccatcaa aatgcggcgt gatctaggcc catgggcgcg ccgctgccgc cctggcctcg
      181 taagcgtcga tgctgccgac cttctgcaac gtctggccga tgtcatcgag gccgttgatc
      241 aggcagtgct tgcgatgcgg atcgatgtcg aaccggatga caccgccatc ggggccacga
      301 atttcctgtg cctcgagatc gatcgtcagc gtcgaattgg caccacgctc ggcatcgtcg
      361 aacagcttgt cgagttcggc ctggctgacc ttgatcggca ggatgccgtt cttgaaggta
      421 ttgttgtaga agatgtcggc gaacgaggtc gagatcacgc agcggatgcc gaaatcgagc
      481 agcgcccagg gcgcatgctc acgggacgag ccgcagccga aattgtcgcc cgccaccaga
      541 atctgggcgt tgcgataggc cggcttgttg agcacgaagt cggggttctc gccgccatcc
      601 tcgcgatagc gcatctccga gaacagcgcc ttgccgagac cggtgcgctt gatcgtcttg
      661 agatactgct tgggaatgat catgtcggtg tcgacattga ccactttcat cggggcggcg
      721 acggcagtga gcttggtgaa aggctgcatg acatacccgg actgttggag aacctttgca
      781 ggcttgatag cgaaatgcgc acagggcgca agccggacgt ggtgcggatg cgcatttcac
      841 cagcctttca gctcacctgc gcctcaatag cctccatatc cgcatccgac aggccgaaat
      901 gatggccgat ctcgtgcacc agcacatgcg tgatgatctt gccgagcgtt tcgtcatgct
      961 cggcccagta gtcgaggatc ggccggcggt agagccagat catgttgggg aactggccgg
     1021 tttccatcat cgccggtgcc tgcgccaggc cgatgccgcg aaacaggccg aggatatcaa
     1081 acggcgtttc cagttgcatg tcatccatgg tctcgtcgtc gggaaaatcg gcgacctgga
     1141 tgatcacgcc ttcgcacaac ttgcgaaatc cggcgggcag ccggtcaaag gccgccgccg
     1201 ccatgacctc gaaggtttcg agcgaggggg cgcgggcagc gggccagggc agagcttgat
     1261 ccgtcatggc ttcaccacag gcgcgcgagt tggccgagtg aaaagatgag atagccgacg
     1321 aacagatagc cgagcgtgcg gccgattcgc ttggccagga tcacgatgcg gtcgttgtcg
     1381 tcgtcggaac cggtctgcat cgcacaaggt taccgtggcg acgcgggaac gcaaccttgc
     1441 cggtgcgctg gcgcacagaa cgcaggtccc tgccgatgcg acgatggacc actggcagcg
     1501 tcggcccccg cgtcatcctc cgttcatcga cagaaggcga ggctggtgct ggcggtctcg
     1561 ccaagacagg catggaggac tatgatcatg gatcgtaggc atctgattgc cggaggcgcg
     1621 ggcctccttc tggccgggct ttttgccggc agcgcttcgg cgcgcatcac ggcgcaaggt
     1681 ccggttgcgg atgaggctgg gccgcaggtc gaggacgtca agcgtggtgg gcgcggccgt
     1741 caccgacgcc ctgcagcaca ggctgcacga gctggtc
//
